LOCUS       DAHHSC010000001        49669 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.001, whole genome shotgun sequence.
ACCESSION   DAHHSC010000001 DAHHSC010000000
VERSION     DAHHSC010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 49669)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49669)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49669
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.001"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            201..2276
                     /gene="lip2"
                     /locus_tag="NBI57_000001"
     CDS             201..2276
                     /gene="lip2"
                     /locus_tag="NBI57_000001"
                     /EC_number="3.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK domain-containing triacylglycerol lipase
                     Lip2/Geh"
                     /protein_id="HCD1821001.1"
                     /translation="MLRGQEERKYSIRKYSIGVVSVLAATMFVVSSHEAQASEKTPTS
                     NAAAQKETLNQPGEQGNAITSHQMQSGKQLDDMHKENGKSGTVTEGKDTLQSSKHQST
                     QNSKTIRTQNDNQVKQDSERQGSKQSHQNNATNNTERQNDQVQNTHHAERNGSQSTTS
                     QSNDVDKSQPSIPAQKVLPNHDKAAPTSTTPPSNDKTAPKSTKAQDATTDKHPNQQDT
                     HQPAHQIIDAKQDDTVRQSEQKPQVGDLSKHIDGQNSPEKPTDKNTDNKQLIKDALQA
                     PKTRSTTNAAADAKKVRPLKANQVQPLNKYPVVFVHGFLGLVGDNAPALYPNYWGGNK
                     FKVIEELRKQGYNVHQASVSAFGSNYDRAVELYYYIKGGRVDYGAAHAAKYGHERYGK
                     TYKGIMPNWEPGKKVHLVGHSMGGQTIRLMEEFLRNGNKEEIAYHKAHGGEISPLFTG
                     GHNNMVASITTLATPHNGSQAADKFGNTEAVRKIMFALNRFMGNKYSNIDLGLTQWGF
                     KQLPNESYIDYIKRVSKSKIWTSDDNAAYDLTLDGSAKLNNMTSMNPNITYTTYTGVS
                     SHTGPLGYENPDLGTFFLMDTTSRIIGHDAREEWRKNDGVVPVISSLHPSNQPFINVT
                     NDEPATRRGIWQVKPIIQGWDHVDFIGVDFLDFKRKGAELANFYTGIINDLLRVEATE
                     SKGTQLKAS"
     gene            complement(2518..3345)
                     /locus_tag="NBI57_000002"
     CDS             complement(2518..3345)
                     /locus_tag="NBI57_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCD1821002.1"
                     /translation="MTEIKYKVITKDAFALPYTIIKAKNQPTKGVIVYIHGGGLMFGK
                     ANDLSPQYIDILTEHYDLIQLSYRLLPEVSLDCIIEDVYASFDAIQSQYSNCPIFTFG
                     RSSGAYLSLLIARDRDIDGVIDFYGYSRINTEPFKTTNSYYAKIAQSINETMIAQLTS
                     PTPVVQDQIAQRFLIYVYARGTGKWINMINIADYTDSKYNIAPDELKTLPPVFIAHCN
                     GDYDVPVEESEHIMNHVPHSTFERVNKNEHDFDRRPNDEAITIYRKVVDFLNAITMV"
     gene            complement(3418..4617)
                     /locus_tag="NBI57_000003"
     CDS             complement(3418..4617)
                     /locus_tag="NBI57_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HCD1821003.1"
                     /translation="MYRYKPLLQSIHLPNGIKISNRFVLSPMTVNASTKEGYITKADL
                     AYAARRSNSAGMQVTGAAYIEPYGKLFEYGFNIDHDACIPGLTNMASTMKQHGSLAII
                     QLAHAGRFSNQAILNFGKVYGPSPMTLHSPIEHVVIAMSHEKINSIIQQYRDATLRAI
                     KAGFDGVEISIAQRLLIQTFFSTFSNRRTDHYGADSLKNRARLCLEVMRAVQEVIDKE
                     APDNFILGFRATPEETRGSDLGYTIDEFNQLIDWVMDVSNIQYLAIASWGRHIYQNTS
                     RTPGDHFGRPVNQIVYEHLAGRIPLIASGGINSPESALDALQHADMVGMSSPFVTEPD
                     FVHKLAEQRPHDINLEFSMADLEDLAIPHAAFKDIVKMMDYGEGLKKHTRDALRQLEQ
                     NYHDSSS"
     gene            4713..4805
                     /locus_tag="NBI57_000004"
     CDS             4713..4805
                     /locus_tag="NBI57_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1821004.1"
                     /translation="MENIFKIELMNGICRSENMNMKKKNKGEKI"
     gene            4919..5920
                     /locus_tag="NBI57_000005"
     CDS             4919..5920
                     /locus_tag="NBI57_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HCD1821005.1"
                     /translation="MVKLSVLDYALIDEGKDAQKALQDSVTLAKLADRLGFKRIWFTE
                     HHNVPAFACSSPELLMMHTLAQTNHIRVGSGGVMLPHYRPYKIAEHFRMMAALYPNRI
                     DLGIGNNPGTTMVKQALDGINPTYDSYDESISLLRDYLTIKDKPSAHTLGVQPHIDHF
                     PEMWLLSSSATSAKIAAELGIGLSVGTFLLPDINAIHAAKDNIDIYKKHFQASTIKMD
                     AKVMASVFVIVADNEAEVAALQHALDVWLLGKLQFAEFEHFPSVDTAQKYKLNDRDKE
                     MIQVHQARIIAGTQEQVKAQLDDFIATFEVDEVLVAPLIPGIEQRCKTLKLLAEIYL"
     gene            5954..6286
                     /locus_tag="NBI57_000006"
     CDS             5954..6286
                     /locus_tag="NBI57_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HCD1821006.1"
                     /translation="MKKLANYLWVEKVGDLYVFSMTPELQDDIGTVGYVEFVSPDEVK
                     VDDEIVSIEASKTVIDVQTPLSGTIIERNTKAEEEPTILNSEKPEENWLFKLDDVDKE
                     AFLALPEA"
     gene            6287..7087
                     /locus_tag="NBI57_000007"
     CDS             6287..7087
                     /locus_tag="NBI57_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-ADP-ribose hydrolase"
                     /protein_id="HCD1821007.1"
                     /translation="METLKSNKARLEYLINDMRRERNDNDVLVMPSSFEDLWELYRGL
                     ANVRPALPVSDEYLAVQDAMLSDLNHQHVTDLKDLKPIKGDNIFVWQGDITTLKIDAI
                     VNAANSRFLGCMQANHDCIDNIIHTKAGVQVRLDCAEIIRQQGRNEGVGKAKKTRGYN
                     LPAKYIIHTVGPQIRRLPVSKMNQDLLAKCYLSCLKLADQHSLNHVAFCCISTGVFAF
                     PQDEAAEIAVRTVESYLKETNSTLKVVFNVFTDKDLQLYKEALNRDAE"
     gene            7077..8021
                     /locus_tag="NBI57_000008"
     CDS             7077..8021
                     /locus_tag="NBI57_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent deacetylase"
                     /protein_id="HCD1821008.1"
                     /translation="MQSSKWNAMSLLMDEKTKQAEVLRTAIDEADAIVIGIGAGMSAS
                     DGFTYVGERFTENFPDFIEKYRFFDMLQASLHPYGSWQEYWAFESRFITLNYLDQPVG
                     QSYLALKSLVEGKQYHIITTNADNAFDAAEYDMTHVFHIQGEYILQQCSQHCHAQTYR
                     NDDLIRKMVVAQQDMLIPWEMIPRCPKCDAPMEVNKRKAEVGMVEDAEFHAQLQRYNA
                     FLEQHQDDKVLYLEIGIGYTTPQFVKHPFQRMTRKNENAIYMTMNKKAYRIPNSIQER
                     TIHLTEDISTLITTALRNDSTTQNNNIGETEDVLNRTD"
     gene            7999..9021
                     /locus_tag="NBI57_000009"
     CDS             7999..9021
                     /locus_tag="NBI57_000009"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HCD1821009.1"
                     /translation="MYLIEPIRNGEYITDGAIALAMQVYVNQHIFLDEDILFPYYCDP
                     KVEIGRFQNTAIEVNQDYIDKHSIQVVRRDTGGGAVYVDKGAVNMCCILEQDTSIYGD
                     FQRFYQPAIKALHTLGATDVIQSGRNDLTLNGKKVSGAAMTLMNNRIYGGYSLLLDVN
                     YEAMDKVLKPNRKKIASKGIKSVRARVGHLREALDEKYRDITIEEFKNLMVTQILGID
                     DIKEAKRYELTDADWEAIDELADKKYKNWDWNYGKSPKYEYNRSERLSSGTVDITISV
                     EQNRIADCRIYGDFFGQGDIKDVEEALQGTKMTREDLMHQLKQLDIVYYFGNVTVESL
                     VEMILS"
     gene            9337..10362
                     /locus_tag="NBI57_000010"
     CDS             9337..10362
                     /locus_tag="NBI57_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde reductase"
                     /protein_id="HCD1821010.1"
                     /translation="MNNKVLVTGGTGFVGMRIISRLLEQGYEVQTTIRDLSKADKVIK
                     TMQDNGISTERLMFVEADLSQDEHWDEAMKDCKYVLSVASPVFFGKTDDAEVMAKPAI
                     EGIQRILRAAEHAGVKRVVMTANFGAVGFSNKDKNSITNESHWTNEDEPGLSVYEKSK
                     LLAEKAAWDFVENENTIVEFATINPVAIFGPSLDAHVSGSFHLLENLLNGSMKRVPQI
                     PLNVVDVRDVAELHILAMTNEQANGKRFIATADGQINLLEIAKLIKEKRPEIAQKVST
                     KKLPDFILSLGAKFNHQAKEGKLLLDMNRNVSNERAKTLLGWEPIATQEEAILAAIDS
                     MAKFHLI"
     gene            complement(10456..11802)
                     /locus_tag="NBI57_000011"
     CDS             complement(10456..11802)
                     /locus_tag="NBI57_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HCD1821011.1"
                     /translation="MQAILNFIVDILSQPAILVALIAFIGLIVQKKPAATITSGTIKT
                     ILGFLILSAGADVVVRSLEPFGKIFQHAFGVQGIVPNNEAIVSLALKDFGTTAALIMV
                     CGMIVNILIARFTNLKYIFLTGHHTFYMAAFLAIILTVSHIKGWLTIVIGALVLGLIM
                     AVLPALLQPTMRKITGNDQVALGHFGSISYFAAGAVGQLFKGKSKSTEEIKFPKGLSF
                     LRESTISISITMALLYFIACLFAGVSYVHESISDGQNFIVFSLIQGVTFAAGVFIILT
                     GVRLILAEIVPAFKGISEKLVPNSKPALDCPIVFPYAQNAVLIGFFVSFITGVIGMFI
                     LFLFGGVVILPGVVAHFFLGATAAVFGNARGGIKGAVAGAALNGILITFLPLLFLPFL
                     GELGGAATTFSDTDFLTVGIVFGNAVKYMGLFGAILFIIIVGATAILLKGRQKPQQ"
     gene            complement(11817..12101)
                     /locus_tag="NBI57_000012"
     CDS             complement(11817..12101)
                     /locus_tag="NBI57_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCD1821012.1"
                     /translation="MKILVVCGHGLGSSFMVEMNAQEALRQLNAPSDIEVEHSDIMTA
                     SPEMADLFICGRDLAENAERLGDVLVLDNILDKAELQQKLSEKLQQLNMI"
     gene            complement(12103..12546)
                     /locus_tag="NBI57_000013"
     CDS             complement(12103..12546)
                     /locus_tag="NBI57_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HCD1821013.1"
                     /translation="MSLDILSTTRIIVKEQVNDWTEAITIASQPLLQEQIIEQGYVQA
                     MIDSVNELGPYIVIAPEIAIAHARPNNDVHQVGLSLLKLNQHVAFCDEDHYASLIFVL
                     SAIDNHSHLSVLQNLATVLGDNQTVQQLLTAKNAQDIKNILKEHD"
     gene            complement(12551..14506)
                     /locus_tag="NBI57_000014"
     CDS             complement(12551..14506)
                     /locus_tag="NBI57_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000954286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HCD1821014.1"
                     /translation="MLSYRQIEILYLLIHEQTFIPINTIANQLGVSPRTIQYDIAYIE
                     QYAETYHYQVSRNKAAGIKVTTAHATLLNELEHNLTNQIHFSKDERLTHIALKLFETT
                     DPVSTKQLAQDVNVSRRTIADDIKMIQAQLDQYHLKLNYVHNKGFNVIGEEDHYRKAY
                     AHFIHQYMKQAAPFIEADIFNSESIALVRRAIIKTLNSENYHLVQSAIDGLIYHILIA
                     IQRLNENFSFDIPINEIDKWRHTNQYAIASKMIENLERSCNVTFPESEIIFITLHLLG
                     SKMTEHTASSITFEYHDLSQNIHELITCVSQELGIDMSKDNKLHTSLITHIKPAIHRI
                     KYDMLQPNPLRQEVMRRYPQIIEAVSKHISPIEQDAAIRFNEDELTYITIHFASSIER
                     VATHKQSMIKVVLLCGSGIGTSQLLKSKLNHLYPEFHIWDAYSIYQLEESRLLQDNID
                     YVISTVPCEISAVPVIHVDPFINQQSRQKLNQIINDSREQRVMKMATDGKSLADLLPE
                     HRIIINEQPLSIESAIAVAVQPLINDGIVYSNYTAAILKQFEQFGSYMVISPHIALIH
                     AGTDYVQNGVGFALTYFTEGIIFGSKANDPVHLVITLATDHPNAHLKALGQLSECLSN
                     DLYRQDFLDGNIFKIKQHIALTMTKEV"
     gene            14718..15137
                     /gene="mepR"
                     /locus_tag="NBI57_000015"
     CDS             14718..15137
                     /gene="mepR"
                     /locus_tag="NBI57_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor MepR"
                     /protein_id="HCD1821015.1"
                     /translation="MEFTYSYLFRMISHEMKQKADQKLEQFDITNEQGHTLGYLYAHQ
                     QDGLTQNDIAKALQRTGPTVSNLLRNLERKKLIYRYVDAQDTRRKNIGLTTSGIKLVE
                     AFTSIFDEMEQTLVSQLSEEENEQMKANLTKMLSSLQ"
     gene            15244..16599
                     /gene="mepA"
                     /locus_tag="NBI57_000016"
     CDS             15244..16599
                     /gene="mepA"
                     /locus_tag="NBI57_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498905.1"
                     /note="MepA (multidrug export protein A), as described
                     originally in Staphylococcus aureus, is a MATE transporter
                     shown to be over-expressed after culturing in the presence
                     of tigecycline, owing to mutation of its repressor MepR,
                     and then to be one of several factors contributing to
                     elevated tigecycline resistance; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MATE transporter MepA"
                     /protein_id="HCD1821016.1"
                     /translation="MKDEQLYYFEKSPVFKAMMHFSLPMMIGTLLSVIYGILNIYFIG
                     FLEDSHMISAISLTLPVFAILMGLGNLFGVGAGTYISRLLGAKDYSKSKFVSSFSIYG
                     GIALGLIVILVTLPFSDQIAAILGARGETLALTSNYLKVMFLSAPFVILFFILEQFAR
                     AIGAPMISMIGMLASVGLNIILDPILIFGFDLNVVGAALGTAISNVAAALFFIIYFMK
                     NSDVVSVNIKLAKPNKEMLSEIFKIGIPAFLMSILMGFTGLVLNLFLAHYGNFAIASY
                     GISFRLVQFPELIIMGLCEGVVPLIAYNFMANKGRMKDVIKAVIMSIGVIFVVCMIAV
                     FTIGHHMVGLFTTDQAIVEMATFILKVTMASLLLNGIGFLFTGMLQATGQGRGATIMA
                     ILQGAIIIPVLFIMNALFGLTGVIWSLLIAESLCALAAMLIVYLLRDRLTVDTSELIE
                     G"
     gene            16703..17143
                     /locus_tag="NBI57_000017"
     CDS             16703..17143
                     /locus_tag="NBI57_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MepB family protein"
                     /protein_id="HCD1821017.1"
                     /translation="MYKSKILLKYIFSEESEVKDLTEEKYNQDYEALTFSFKEETYQS
                     RLAKKTPTKAGYFVTCWTKDENNCNQPYSKEAFADYLMIIVIDEELSGYFLFPRELLV
                     EKGILTTFEHKGKMAFRVYPKWCNQLNKTAGQTQKWQCKYFFEY"
     gene            complement(17226..18584)
                     /gene="glpT"
                     /locus_tag="NBI57_000018"
     CDS             complement(17226..18584)
                     /gene="glpT"
                     /locus_tag="NBI57_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate transporter"
                     /protein_id="HCD1821018.1"
                     /translation="MNFLKPAKHIKPLPENQIDDTYKRLRLQVFLGIFIGYAGYYLLR
                     KNFSLAMPALQEQGFTKAELGFALSAVSIAYGFSKFFMGTVSDRSNARIFLVLGLALT
                     AIVNLLMGFVPFFTSGIGIMFVLLFLNGWFQGMGWPPSGRVLVHWFSVSERGSKTALW
                     NVAHNVGGGIMAPIAAWGITTTAFINFGYLKGFEGVFIYPALLALIIAAISYVLIRDT
                     PQSQGLPPIEIYKNDFATSDKKTLETELTTKEILFKYVLNNKWVWAIAFANIFVYFVR
                     YGVLDWAPVYLSEEKHFDLKASGWAYFLYEWAGIPGTLLCGYISDKLFKGRRGPAGFF
                     FMLGVTVFVLIYWLNPPGNAWLDNVSLIAIGFLIYGPVMLIGLQALDYVPKKAAGTAA
                     GLTGLFGYLFGAVMANIVLGAVVDKFGWDVGFILLTAISVFAMLSFILTWNKVGQETV
                     HH"
     gene            18896..19822
                     /locus_tag="NBI57_000019"
     CDS             18896..19822
                     /locus_tag="NBI57_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCD1821019.1"
                     /translation="MNIVGHHHISMYTKDAKRNKDFYTNVLGLRLVEKSVNQDNPSMY
                     HLFYGDEVGTAGTILSFFEIPHAGHKQPGTETIYRFSLLVPNQAALHYFEKRLDNNGI
                     TSERLYYLGQEGVVFKDEDDLEIILLVNDSFEVPHQWQHNAYSEIPQAYQILGIGPVE
                     LRVRNAARTVEFLENVLGYRKRDNKSFDVLTLAPQGLYSDFVVIEQQGQRERPGRGYI
                     HHIAINTPQMSDLDAIYKKLQQQTQSNSGIIDRYFFKSLYYRHNSIMYEFATEAPGFT
                     IDTPLEQLGSQLNLPDFLEAEREQIESKLHEI"
     gene            19836..20897
                     /locus_tag="NBI57_000020"
     CDS             19836..20897
                     /locus_tag="NBI57_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HCD1821020.1"
                     /translation="MAKLEMNKNTPLEFGLYSLGDHLLNPFKGEKVSYEQRINEIIEA
                     SKLADETGIDVFAVGESHQEHFTTQAHTVVLGAIAQATKHIKVSSSSTIISATDPVRV
                     FEDFATLDLISHGRAEIVAGRASRTGIFDLFGYDLKDYDELFEEKLGLLLELNKTERI
                     TWSGKYRPELRNMKIFPRPIDNILPIWRAVGGPPASAIKAGKQGVPMMITTLGGPAMN
                     FKGSIDAYRQAATEAGFDASHKSLPVSTASLFYTAETTQDAMREFYPHLNTGMSFIRG
                     VGYPKQQFANSSDYREALMVGSPQQIIEKILYQHELYGHQRFMAQLDFGGVPFENVMK
                     NIELIGNEIIPAIKKHLSK"
     gene            20911..21477
                     /locus_tag="NBI57_000021"
     CDS             20911..21477
                     /locus_tag="NBI57_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HCD1821021.1"
                     /translation="MNIVLLSGSTVGSKTRIAMDDLKNELEVINEGHQIELMDLRELE
                     LEFSVGKNYLDTTGDVYKLTTSLMQADVIFIGFPIFQASIPGALKNVFDLLPVNAFRD
                     KVIGLVATAGSSKHYLIPEMHLKPILSYMKAHTMQTYVFIEEKDFSNQQIVNDDVVFR
                     LKALAQFTMRTAKVQQQVFEEENNQYDF"
     gene            complement(21536..22531)
                     /locus_tag="NBI57_000022"
     CDS             complement(21536..22531)
                     /locus_tag="NBI57_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family putative sulfate export transporter"
                     /protein_id="HCD1821022.1"
                     /translation="MASLKNKHFMIGLSLTFIVALFSFLAAKLPILDKVGALTIAILI
                     AILYRHFRGYPEQYSSGITFSSKYLLRFAIILYGLKLNIFDIIGQGSKLLAIDVGVVI
                     FSIVMMLFVNKLLHGDKNIALLLGVGTGVCGAAAIAAVAPIFKSREKDTAISIGIIAL
                     IGTIFSLIYTAIYAIFSMTTNVYGAWSGVSLHEIAHVVLAGGFGGSDALKIALLGKLG
                     RVFLLIPLTIVLILIMRFRSSESSSKGRISIPYFLIGFVIMALVNTYVTIPSVLLNIL
                     NTISTICLLMAMVALGLNVAFKDLKNRALKPLMTIIITSICLSSLAFIVVHWLYS"
     gene            22896..23441
                     /locus_tag="NBI57_000023"
     CDS             22896..23441
                     /locus_tag="NBI57_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCD1821023.1"
                     /translation="MFGMKVNEQITLKILEAHDTEALFNLVNRSRNSLREWLPWVDAT
                     EQPSDTRAFIKRGLLQFADGNGFQCGIWYEGTLVGVIGLHEINHMHRKTSLGYYLDKQ
                     FEGHGIMTQAVEALIKYCFEELDLNRIEISAAVNNEKSRAIPERLGFTREGMLRDNEL
                     LNGIYSSSYIYSLLKSEYDQK"
     gene            23708..24562
                     /locus_tag="NBI57_000024"
     CDS             23708..24562
                     /locus_tag="NBI57_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1821024.1"
                     /translation="MKKLTTLLLASTLLIAACGNDDSKKDDSKTSKKDDGVKAELKQA
                     TKAYDKYTDEQLNEFLKGTEKFVKAIENNDMAQAKALYPKVRMYYERSEPVAEAFGDL
                     DPKIDARLADMKEEKKEKEWSGYHKIEKALYEDKKIDDVTKKDAQQLLKDAKELHAKA
                     DTLDITPKLMLQGSVDLLNEVATSKITGEEEIYSHTDLYDFKANVEGAQKIYDLFKPI
                     LEKKDKKLSDDIQMNFDKVNQLLDKYKDNNGGYESFEKVSKKDRKAFADAVNALGEPL
                     SKMAVITE"
     gene            24559..25788
                     /gene="efeB"
                     /locus_tag="NBI57_000025"
     CDS             24559..25788
                     /gene="efeB"
                     /locus_tag="NBI57_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deferrochelatase/peroxidase EfeB"
                     /protein_id="HCD1821025.1"
                     /translation="MTNYEQVNDSTQFSRRTFLKMLGIGGAGVAIGASGVGSMWSFKS
                     MFNTPEDPEKDAYEFYGKVQPGITTPTQKTCNFVALDLKSKDRDAIKAMFKKWTVMAD
                     RMMDGDTVGKTSNNPLMPPVDTGESIGLGASKLTITFGISKSLMKKIGLSSKIPDAFK
                     DLPHFPNDQLIDDYSDGDIMIQACSNDSQVSFHAVHNLVRPFRDIVKVRWAQSGFISA
                     KGKETPRNLMAFKDGTINPRKSNQLKDYVFIDDGWAKHGTYCVVRRIQIHIETWDRTA
                     LEEQEATFGRKRHSGAPLTGGKEFDEIDLKAKDSHGEYIIDKDAHTRLAKEANTSILR
                     RAFNYVDGTDDRTGNFETGLLFIAFQKATKQFIDIQNNLGSNDKLNEYITHRGSASFL
                     VLPGVSKGGYLGETLFD"
     gene            25769..27481
                     /locus_tag="NBI57_000026"
     CDS             25769..27481
                     /locus_tag="NBI57_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000683714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FTR1 family iron permease"
                     /protein_id="HCD1821026.1"
                     /translation="MKHYLTKFVAMLITAAMVCSFGLLKSQAAEQQSISDVYSVITDA
                     KSALSNNSISNDNKQKAIEQVVSAVKKLSLEDNSEGNAVKSDVRKLEDAKANDNQKDT
                     LSQLTKSLIAYEEKLASKDAGSKIKLLQQQVDAKDAAMTKAIKDKNKAELESLNNSLN
                     QIWTSNETVIRNYDANQYGQIEVALLQLRIAIHKSPLDTAKVSHAWTTFKSNIDHVDK
                     KSDTSANDQYHVSQLNDALEKAIKAIDDNQLSDADAALTHFIEIWPYVEGQIQTKDGA
                     LYTKIEDKIPYYQSVLDEHNKSHVKDGLVDINNQIKEVVGHSYSFVDVMIIFLREGLE
                     VLLIVMTLTTMTRKVKDNKGTASVIGGAIAGLVLSIILAITFVETLGNSGILRESMEA
                     GLGIVAVILMFIVGVWMHKRSNAKRWNDMIKNMYANAISNGNLVLLATIGLISVLREG
                     VEVIIFYMGMIGELATKDFIIGIALAIVILIVFAVLFRFIVRLIPIFYIFRVLSIFIF
                     IMGFKMLGVSIQKLQLLGAMPRHVIEGFPTINWLGFYPSYEPLIAQAAYIMVVAILIF
                     KFKK"
     gene            complement(27722..28384)
                     /gene="tatC"
                     /locus_tag="NBI57_000027"
     CDS             complement(27722..28384)
                     /gene="tatC"
                     /locus_tag="NBI57_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006191819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HCD1821027.1"
                     /translation="MLLSFVVTVIVVYVSSFWWMTPFITYITRAHVSLHAFSFTEMIQ
                     IYVMIIFFIAFCFISPVMFYQLWAFIAPGLHNNERQFIYKYSFFSVLLFCAGVAFAFY
                     VGFPIIIQFALKLSLTLNISPVIGFKAYLVELIRWLFTFGILFQLPILFIGLAKFGLI
                     DITSLKHYRKYIYFACFVLASIIAPPDLTLNILLTLPLILLFEFSMFIVKFTCRGKPP
                     TH"
     gene            complement(28440..28664)
                     /locus_tag="NBI57_000028"
     CDS             complement(28440..28664)
                     /locus_tag="NBI57_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HCD1821028.1"
                     /translation="MIIMITNTFILGITGPTSLVVISIIALIIFGPKKLPQFGRAIGS
                     TLKEFKSATEDLDKESHDTPSKESKQQREQ"
     gene            complement(28764..29153)
                     /locus_tag="NBI57_000029"
     CDS             complement(28764..29153)
                     /locus_tag="NBI57_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1398 family protein"
                     /protein_id="HCD1821029.1"
                     /translation="MAFTLSAIQQAHQQFTGVDFPKLFKAFKDMGMTYNIVNIQDGTA
                     TYVHQSEDDIVTSSVKSNHPVAQKSNKTIVQDVLTRHQQGQTDFETFCDEMAEAGIYK
                     WHIDIQAGTCTYIDLKEQAIISELIPQ"
     gene            29393..29596
                     /locus_tag="NBI57_000030"
     CDS             29393..29596
                     /locus_tag="NBI57_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCD1821030.1"
                     /translation="MRNRLKELRARDGLNQTQLAKLAGVSRQTISLIERNNFMPSVLT
                     AIKIARIFNETVETVFIIEEDEA"
     gene            29593..30306
                     /locus_tag="NBI57_000031"
     CDS             29593..30306
                     /locus_tag="NBI57_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3169 family protein"
                     /protein_id="HCD1821031.1"
                     /translation="MKILRYIGYLLLGGIVGGIIGGILGNFDGLGIENLTFATYNNVV
                     VISIVATMIIILVEAIVLMNQRRALKYKRLVDEEVDIDATDQYELLANRYVLNGSILS
                     VIQTIIAFVVLLIFVVGQAEANAMLFFLIPFFASAIFNTQFTLFNRKFDDRMPKIADK
                     NYTEKRLEILDEGERHIELIALFKTYAINLSILILAIIFIGSYSIATGINQSFSLLLI
                     IAIFIYNAFSYLLKRRRFY"
     gene            30331..31173
                     /locus_tag="NBI57_000032"
     CDS             30331..31173
                     /locus_tag="NBI57_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCD1821032.1"
                     /translation="MTTLLNVDRVNKQYKDSDFKLQDASLTISTNETVGLIGKNGSGK
                     STLINILVGNRHKDNGSIAFFGEEHAADDVEYKEHIGVVFDDLRVPNKLTIKDIDKVF
                     QSIYTTWNSQKFFDLIKYFELPLQTKIKTFSRGMRMKIALTIALSHDVKLLILDEATA
                     GMDVSGREEVMELLEDFVAQGGGILISSHISEDIEQLADKLVFMKDGRMILTEQKDIL
                     LAQYGIVTTGDKDVEIPKHLIIASRLSKGKYQILVKDYAEIENAEPLKHIDDATKIIM
                     RGEV"
     gene            31173..31802
                     /locus_tag="NBI57_000033"
     CDS             31173..31802
                     /locus_tag="NBI57_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 transporter permease"
                     /protein_id="HCD1821033.1"
                     /translation="MKGMFLSSFYATRKQTYIYFIVAIIAAGYFAVFNPLMSSAMAGV
                     MLITPITDNIKHEKDSRWMYYVSTLPVKRSDYIKSYFAFYLILFGASLMIGLVVTTIV
                     TQSVMIGIMSGLMSFGIIGAYSIIFPLTFKFGAENSNVIMIVASILLLFSFVVFFFIY
                     GMVSGASALEFEKISTEGWLVVIVYAVIGIVITSVSYILSIKIFNKQEL"
     gene            complement(32189..33322)
                     /locus_tag="NBI57_000034"
     CDS             complement(32189..33322)
                     /locus_tag="NBI57_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low temperature requirement protein A"
                     /protein_id="HCD1821034.1"
                     /translation="MVNALTPKHLAEKRAGFHELFFDLIFVYAIQKIAHVILTTQNGS
                     ISADLFFKYIVMSLFLWLMWSHQTFFTNRFGQVTFKDVSFMMFNMFIMVFLSNSLYPD
                     FEKTFFPFFLCVAIMYLSIGLQYLLHIRTGLDYGDKRTCQAFASVAFVISFLSFLSLV
                     LPQSIHYIPGFLGVFIAATGLIPFQKYLVLSPVNMMHLVERFSLLTIIIFGEVLVGLA
                     SSSFSIDHFSYIYIFQFMILISLFGVYWIITENYINHKLSSIGFRLSYTHLLINIALG
                     VINAAIVFSNNNKLNDLFEINMMYISVLIFYIGLWLITPYFHNELTNAKYISSSLGIL
                     VVSYIISLIFKGHDQVMIISVSVATFCIMLIYFKNQRLRNKHE"
     gene            33861..35042
                     /locus_tag="NBI57_000035"
     CDS             33861..35042
                     /locus_tag="NBI57_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HCD1821035.1"
                     /translation="MTRVVLAAAYRTPIGVFGGAFKDVPAYDLGATLIEHIIKETGLN
                     PSEIDEVIIGNVLQAGQGQNPARIAAMKGGLPETVPAFTVNKVCGSGLKSIQLAYQSI
                     VTGENDIVLAGGMENMSQSPMLVNNSRFGFKMGHQSMVDSMVYDGLTDVFNQYHMGIT
                     AENLVEQYGISREEQDTFAVNSQHKAVRAQQNGEFDSEIVPVSIPQRKGEPILVTKDE
                     GVRENVSVEKLSRLRPAFKKDGTVTAGNASGINDGAAMMLVMSEDKAKELNIEPLAVL
                     DGFGSHGVDPSIMGIAPVGAVEKALKRSKKELSDIDVFELNEAFAAQLLAVDRELKLP
                     PEKVNVKGGAIALGHPIGASGARVLVTLLHQLNDEVETGLTSLCIGGGQAIAAVVSKY
                     K"
     gene            complement(35125..35877)
                     /locus_tag="NBI57_000036"
     CDS             complement(35125..35877)
                     /locus_tag="NBI57_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HCD1821036.1"
                     /translation="MTQYPLWQQLNTLKQATWVDLTHTFDPEIPRFSEFEKGEVSTLF
                     TVKDHGFYVQRWNIVTQYGTHIDAPIHFVENKRYLEDIDLKELVLPLIVLDFSTEVAN
                     NNDFIVTRAHIEAWEKEHGTIEPGTFVALRTDWSKRWPNIEKFENKDANGQQHAPGWG
                     LDALKYLIEERRVEAVGHETFDTDASVDVVKNGDLVGERYILGQDKYQVELLTNLDQL
                     PTRGAIIYAISPKPKDAPGFPVRAFAIKPSND"
     gene            complement(35920..38148)
                     /gene="metE"
                     /locus_tag="NBI57_000037"
     CDS             complement(35920..38148)
                     /gene="metE"
                     /locus_tag="NBI57_000037"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HCD1821037.1"
                     /translation="MTTIKTSNLGFPRLGRKREWKKAIESYWAKKISKEELDQTLTDL
                     HKENLLLQKYYHLDSIPVGDFSLYDHILDTSLLFNIIPERFQGRTIDDDLLFDIARGN
                     KDHVASALIKWFNTNYHYIVPEWDNVEPKVSRNVLLDRFKYAQSLNVNAHPVIVGPIT
                     FVKLSKGGHQTFEEKVKTLLPLYKEVFESLIDAGAEYIQVDEPILVTDDSESYENITR
                     EAYDYFEKAGVAKKLVIQTYFERAHLKFLSSLPVGGLGLDFVHDNGYNLKQIEAGDFD
                     KSKTLYAGIIDGRNVWASDIEAKKVLIDKLLAHTNELVIQPSSSLLHVPVSLDDETLD
                     TSVGEGLSFATEKLDELDALRRLLNQNDSVKYDKLKARYERFQNQSFKNLDYDFESVR
                     TSRQSPFAQRIEQQQKRLNLPDLPTTTIGSFPQSREVRKYRADWKNKRITDEAYETFL
                     KNEIARWIKIQEDIGLDVLVHGEFERNDMVEFFGEKLQGFLVTKFGWVQSYGSRAVKP
                     PIIYGDVKWTAPLTVDETVYAQSLTDKPVKGMLTGPVTILNWSFERVDLPRKVVQDQI
                     ALAINEEVLALEAAGIKVIQVDEPALREGLPLRSEYHEQYLKDAVLSFKLATSSVRDE
                     TQIHTHMCYSQFGQIIHAIHDLDADVISIETSRSHGDLIKDFEDINYDLGIGLGVYDI
                     HSPRIPTKEEITTAINRSLQQIDRSLFWVNPDCGLKTRKEEEVKDALTVLVNAVKAKR
                     QE"
     gene            complement(38145..39986)
                     /locus_tag="NBI57_000038"
     CDS             complement(38145..39986)
                     /locus_tag="NBI57_000038"
                     /EC_number="1.5.1.20"
                     /EC_number="2.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional homocysteine
                     S-methyltransferase/methylenetetrahydrofolate reductase"
                     /protein_id="HCD1821038.1"
                     /translation="MSQFLTQLKDNVLVADGAIGTILYSEGLDTCPEAYNLSHPDKVE
                     RIHRSYIEAGADVIQTNTYGANFEKLKRFGLEDKVKAIHQAAVRIAKKAANKDTYILG
                     TVGGFRGIKQEDISLQTILYHTEIQIDTLIEEGVDALLFETYYDLEELTNVISRTRKK
                     YDIPIIAQLTASNTNYLVNGQAINEGLKQLVQCGANIVGLNCHHGPHHMQESFTHIEL
                     PEHAFLSCYPNASLLDIENSEFKYSDNAQYFGQVAQNLIREGVRLIGGCCGTTPEHIK
                     FIKESIQTLKPVNDKKVIPIPTKALFNPSQNKVRQSLTSKVQERPTVIIELDTPKHLD
                     TDRFFENIAKLDKANVDAVTLADNSLATVRISNIAAASLIKQYYNIEPLVHITCRDRN
                     LIGLQSHLLGLSLIGVNEILAITGDPSKVGHLPGATNVYDVNSKGLTELALRFNQGIN
                     TDGDALKKRTHFNIAGAFNPNVRKLDGAVKRLEKKIESGMSYFITQPVYSKEKIIEIY
                     HATKHLNKPFFIGIMPIASYKNALFLHNEVPGIKMSDEILQQFEAVKDDKAKTRELSL
                     KLSKDLIDTVHEYFNGLYIITPFQNVEDSLELAAYSKSITAHKEAIL"
     gene            complement(39955..41115)
                     /locus_tag="NBI57_000039"
     CDS             complement(39955..41115)
                     /locus_tag="NBI57_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HCD1821039.1"
                     /translation="MTLSKETEVIFDWRRGVEYHSANPPLYDSSTFHQTSLGGDVKYD
                     YARSGNPNRELLEEKLARLEQGKFAFAFASGIAAISAVLLTFKSGDHVILPDDVYGGT
                     FRLTEQILNRFNIEFTTVDTTKLEQIEGAIQSNTKLIYIETPSNPCFKITDIKAVSKI
                     AEKHELLVAVDNTFMTPLGQSPLLLGADIVIHSATKFLSGHSDLIAGAVITNNEAISE
                     ALYLIQNGTGNMLSAQDSWTLAKHLKTFPIRFKQSVENAQKIVSFLIKQDEISEVYYP
                     GLTTAHLEQAKNGGAVIGFRLADESKAQQFVDALTLPLVSVSLGGVETILSHPATMSH
                     AALPEEVRQERGITFGLFRLSVGLEDPDELIADIKYALKEAFNESIPHTIER"
     gene            complement(41112..42215)
                     /locus_tag="NBI57_000040"
     CDS             complement(41112..42215)
                     /locus_tag="NBI57_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HCD1821040.1"
                     /translation="MKDTQLAQITLTDDSTGAIANPIHLSTAYKHPKLGQSTGFDYTR
                     TKNPTRSTFETCFAKLEHGIASFATSSGMSAIQLICNLFKPHDEILVSFDLYGGTFRL
                     FEFYEQQYDIKFKYVDFTDYEQVEKEITDKTVALFIEPISNPQMIAIDVKPYYQLCKA
                     KGLLSIIDNTFLTPYLSTPLAEGADIVLHSATKYIGGHNDVLAGVVTVKDESLAQQLF
                     DFHNMTGATLSPIDSYLLLRGLKTLHLRIERAQSNARKLAKKCQSLQAIDEVLYSGQT
                     GMLSLRLNKAYSVAKLLENLDICIFAESLGGTETLVTFPYTQTHVDMPDAEKDKRGID
                     EYLIRLSLGVENYEDIERDIIQALDKAQIGEIV"
     misc_binding    complement(42279..42648)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            42877..43722
                     /locus_tag="NBI57_000041"
     CDS             42877..43722
                     /locus_tag="NBI57_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HCD1821041.1"
                     /translation="MSELSKSEDQRITKTKDEQIKQIDISDIKPNPYQPRKTFDENHL
                     NDLADSIKQYGILQPIVLRKTVQGYYIVVGERRFRASKIAGLKYVSAIIKDLTDEDMM
                     ELAVIENLQREDLNAIEEAESYQRLMTDLKITQQEVAKRLSKSRPYIANMLRLLHLPK
                     KIADMVKDGRLTSAHGRTLLAIKDEQQMLRLAKRVVKEKWSVRYLENHVNELKNVSSK
                     SETDKVDITKPKFIKQQERQLREQYGTKVDISIKKSVGKISFEFDSQDDFVRIIEQLN
                     RRYGK"
     gene            43876..44757
                     /locus_tag="NBI57_000042"
     CDS             43876..44757
                     /locus_tag="NBI57_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HCD1821042.1"
                     /translation="MDQVMSIISSLFEPLTKIETYENIATKIAMIVIYIIVALIVIKI
                     LNKMIEQGFKIQNKSKKSNKKRSKTLISLVQNVVKYIVWFIVITTILSKFGISVEGVI
                     ASAGVVGLAVGFGAQTIVKDVITGFFIIFESQFDVGDYVKINNGGTTVAEGTVKSIGL
                     RSTRINTISGELTILPNSSMGEITNYSITNGTAIVKIPVSVDENIDNVEKKLNKLFTS
                     LRSKYYLFVSDPVVIGIDAFEDTRVILRVSAETIPGEGFSGARIIRKEVQKMFLQEGI
                     KTPQPIMTPFNHSENGV"
     gene            44787..44990
                     /locus_tag="NBI57_000043"
     CDS             44787..44990
                     /locus_tag="NBI57_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HCD1821043.1"
                     /translation="MASKYGINDIVEMKKQHACGTNRFKIIRMGADIRIKCENCQRSI
                     MIPRQTFDKKLKKIIVSQDDTQR"
     gene            45002..46099
                     /gene="ychF"
                     /locus_tag="NBI57_000044"
     CDS             45002..46099
                     /gene="ychF"
                     /locus_tag="NBI57_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019469841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HCD1821044.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGALAANYPFATIDPNVGIV
                     EVPDARLLKLEEMVQPKKTLPTTFEFTDIAGIVKGASKGEGLGNKFLSHIREVDAICQ
                     VVRAFDDDNVTHVAGRVDPIDDIEVINMELVLADLESVEKRLPRIEKLARQKDKTAEM
                     EVRILTTIKEALENGKPARSIDFNEEDQKWVNQAQLLTSKKMLYIANVGEDEIGDDDN
                     DKVKAIREYAAQEDSEVIVISAKIEEEIATLDDEDKEMFLEDLGIEEPGLDRLIRTTY
                     ELLGLSTYFTAGVQEVRAWTFKQGMTAPQCAGIIHTDFERGFIRAEVTSYDDYVQYGG
                     ESGAKEAGRQRLEGKEYIMQDGDIVHFRFNV"
     gene            complement(46185..46376)
                     /locus_tag="NBI57_000045"
     CDS             complement(46185..46376)
                     /locus_tag="NBI57_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1821045.1"
                     /translation="MNLNIDWSKDFQEFQEILNSGIHPEWLYCAKANLVLEPAYTGEG
                     KQFFSTQDIIYASKIIPFF"
     gene            46620..46916
                     /locus_tag="NBI57_000046"
     CDS             46620..46916
                     /locus_tag="NBI57_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001831345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HCD1821046.1"
                     /translation="MRTYEVMYIVRPNIEEDAKKALVERFNGILATEGAEVLEAKDWG
                     KRRLAYEINDFKDGFYNIVRVKSDNNKATDEFQRLAKISDDIIRYMVIREDEDK"
     gene            46937..47440
                     /gene="ssb"
                     /locus_tag="NBI57_000047"
     CDS             46937..47440
                     /gene="ssb"
                     /locus_tag="NBI57_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HCD1821047.1"
                     /translation="MLNRVVLVGRLTKDPEYRTTPSGVSVATFTLAVNRTFTNAQGER
                     EADFINCVVFRRQADNVNNYLSKGSLAGVDGRLQSRNYENQEGRRVFVTEVVCDSVQF
                     LEPKNAQQNGGQRQQNEFQDYGQGFGGQQSGQNNSYNNSSNTKQSDNPFANANGPIDI
                     SDDDLPF"
     gene            47492..47734
                     /gene="rpsR"
                     /locus_tag="NBI57_000048"
     CDS             47492..47734
                     /gene="rpsR"
                     /locus_tag="NBI57_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002484306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HCD1821048.1"
                     /translation="MAGGPRRGGRRRKKVCYFTANGITHIDYKDTELLKRFISERGKI
                     LPRRVTGTSAKYQRMLTTAIKRSRHMALLPYVKEEQ"
     gene            complement(48011..48949)
                     /locus_tag="NBI57_000049"
     CDS             complement(48011..48949)
                     /locus_tag="NBI57_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Abi family protein"
                     /protein_id="HCD1821049.1"
                     /translation="MLNFDEQIAKLKQMNIFFNIIDTEKANEILRKNNYFFKLAYFRK
                     NFEKKNGGYFIEFAYLSDLATIDMKLRYTMLHLTLDIEHSLKCLVLKLITENNQEDGY
                     KIIDEFLCIDKSYSNSNFDTNSRTPEEVMETKIKNKNEIFKHMNKRGQLPEKLNKYYQ
                     NPPAWVCIEFMQLGQFVSFLNFYYKKYNDEELRVANILMPLVKNIRNKSAHNQPIIAN
                     LNYDSRSPQYLFEKGNNIGISRNMFGIKNFIDTFATLELHNQVCSNAIIQARYHDLDQ
                     LQKRYKRNESYYNNALAIKRFFIALDKIIDFNRPKV"
     gene            complement(48988..49669)
                     /locus_tag="NBI57_000050"
                     /pseudo
     CDS             complement(48988..49669)
                     /locus_tag="NBI57_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000270135.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
BASE COUNT        16765 a         7571 c         9568 g        15765 t
ORIGIN      
        1 acgccacaac taaaagtgtt tataaattat ttggaaatgc acatatttgt aaatgattag
       61 tatcgattta atatcgtatt attaaatttt tattaatttt gtagtcttaa tcaaaaaata
      121 atatatgtca tgttatattg aaggtgcagt tgtttttcat tctcaagagg gggtcaaaaa
      181 aatacttttg aggtgattat atgttaagag gacaagaaga aagaaagtat agtattagaa
      241 agtattcaat aggcgtggtg tcagtgttag cggctacaat gtttgttgtg tcatcacatg
      301 aagcacaagc ctcggaaaaa acaccaacgt ccaatgcagc ggcacaaaaa gaaacactaa
      361 atcaaccggg agaacaaggg aacgcgataa cgtcacatca aatgcagtca ggaaagcaat
      421 tagacgatat gcataaagag aatggtaaaa gtggaacagt gacagaaggt aaagacacgc
      481 ttcaatcatc gaagcatcaa tcaacacaaa atagtaaaac aatcagaacg caaaatgata
      541 atcaagtaaa gcaagattct gaacgacaag gttctaaaca gtcacaccaa aataatgcga
      601 ctaataatac tgaacgtcaa aatgatcagg ttcaaaatac ccatcatgct gaacgtaatg
      661 gatcacaatc gacaacgtca caatcgaatg atgttgataa atcacaacca tccattccgg
      721 cacaaaaggt attacccaat catgataaag cagcaccaac ttcaactaca cccccgtcta
      781 atgataaaac tgcacctaaa tcaacaaaag cacaagatgc aaccacggac aaacatccaa
      841 atcaacaaga tacacatcaa cccgcgcatc aaatcataga tgcaaagcaa gatgatactg
      901 ttcgccaaag tgaacagaaa ccacaagttg gcgatttaag taaacatatc gatggtcaaa
      961 attccccaga gaaaccgaca gataaaaata ctgataataa acaactaatc aaagatgcgc
     1021 ttcaagcgcc taaaacacgt tcgactacaa atgcagcagc agatgctaaa aaggttcgac
     1081 cacttaaagc gaatcaagta caaccactta acaaatatcc agttgttttt gtacatggat
     1141 ttttaggatt agtaggcgat aatgcacctg ctttatatcc aaattattgg ggtggaaata
     1201 aatttaaagt tatcgaggaa ttgagaaagc aaggctataa tgtacatcaa gcaagtgtaa
     1261 gtgcatttgg tagtaactat gatcgcgctg tagaacttta ttattacatt aaaggtggtc
     1321 gcgtagatta tggcgcagca catgcagcta aatacggaca tgagcgctat ggtaagactt
     1381 ataaaggaat catgcctaat tgggaacctg gtaaaaaggt acatcttgta gggcatagta
     1441 tgggtggtca aacaattcgt ttaatggaag agtttttaag aaatggtaac aaagaagaaa
     1501 ttgcctatca taaagcgcat ggtggagaaa tatcaccatt attcactggt ggtcataaca
     1561 atatggttgc atcaatcaca acattagcaa caccacataa tggttcacaa gcagctgata
     1621 agtttggaaa tacagaagct gttagaaaaa tcatgttcgc tttaaatcga tttatgggta
     1681 acaagtattc gaatatcgat ttaggattaa cgcaatgggg ctttaaacaa ttaccaaatg
     1741 agagttacat tgactatata aaacgcgtta gtaaaagcaa aatttggaca tcagacgata
     1801 atgctgccta tgatttaacg ttagatggct ctgcaaaatt gaacaacatg acaagtatga
     1861 atcctaatat tacgtatacg acttatacag gtgtgtcttc acatactggt ccattaggtt
     1921 atgaaaatcc tgatttaggt acatttttct taatggatac aacgagtaga attattggtc
     1981 atgatgcaag agaagaatgg cgtaaaaatg atggtgtcgt accagtgatt tcgtcgttac
     2041 atccatccaa tcaaccattt attaatgtta cgaatgatga acctgccaca cgcagaggta
     2101 tctggcaagt taaaccaatc atacaaggat gggatcatgt cgattttatc ggtgtggact
     2161 tcctggattt caaacgtaaa ggtgcagaac ttgccaactt ctatacaggt attataaatg
     2221 acttgttgcg tgtggaagcg actgaaagta aaggaacaca attgaaagca agttaaattc
     2281 atcttctgaa tttaataggc tatgtaaatc gtgctgttat catggcacat cagatataag
     2341 tagcatcaca gtgttgaatt tcaaaatagt aaagtgaaat aaagcgcctg tctcattagc
     2401 gaaaactaaa gggacaggcg tatctgttta tgagcttaat aaattgtatg aataatatgg
     2461 ttgatcgaat aactgtttat catgatgata aattgagttt tttaaaataa tgatatatta
     2521 caccattgtt atagcgttta agaaatcaac aactttacga taaatagtga ttgcttcgtc
     2581 attaggtcta cgatcaaaat catgctcgtt tttattcacg cgttcaaatg ttgaatgtgg
     2641 aacatgattc atgatatgtt cgctttcctc aacgggaaca tcataatcgc cattacaatg
     2701 cgcaatgaaa acaggtggaa gtgttttaag ttcatctggt gcaatattat attttgaatc
     2761 agtataatca gcaatgttaa tcatatttat ccatttacct gtgccacgtg cataaacgta
     2821 gattaaaaaa cgttgtgcga tttgatcttg aacaaccggt gttggtgaag tgagttgtgc
     2881 aatcattgtt tcgtttatgc tttgagctat ttttgcgtaa taactattag ttgttttaaa
     2941 aggttcagtg ttgatgcgac tataaccata aaaatcaata acaccatcaa tatctctgtc
     3001 tcgtgcaatt aatagactta aatatgcacc tgatgatctg ccaaaggtaa aaatagggca
     3061 attagaatat tgtgattgaa tcgcatcgaa tgatgcgtag acatcctcaa taatgcaatc
     3121 gagacttact tctggtaata aacgataact tagttgaatt aaatcgtaat gttccgtaag
     3181 gatatcgata tactgtgggg ataaatcgtt agctttaccg aacattaatc caccaccgtg
     3241 gatgtagaca ataacgcctt ttgttggttg attttttgct ttaataattg tgtaaggtaa
     3301 tgcaaatgca tctttagtaa ttactttata tttaatttca gtcacgattt aataggctcc
     3361 ttatttttga tattgatgtc attataacac tgtcttaaat ttccatgaaa aatagtctta
     3421 agacgatgag tcatgataat tctgttccaa ttgacgtaaa gcgtcacggg tatgcttctt
     3481 tagaccttcc ccataatcca tcattttaac aatatcttta aaagcagcat gtggaatggc
     3541 taaatcttct aaatctgcca tagaaaattc aagattgata tcatgtggtc gctgttcagc
     3601 aagtttatgc acaaagtcag gttctgtgac aaaaggcgaa gacatgccga ccatatctgc
     3661 atgttgtaaa gcatctaaag cagactctgg agaattaatc ccgccacttg caattaaagg
     3721 gatacgacct gctaaatgtt catagacaat ttggttaact ggtcgaccga aatgatcacc
     3781 tggtgtacga gacgtatttt gataaatatg tcgaccccag ctagcgattg ctaagtattg
     3841 gatgtttgaa acgtccatga cccaatcgat taattggttg aactcgtcaa tggtatatcc
     3901 taaatcactg cctctggttt cttctggcgt tgctcgaaat cctaaaataa aattgtcagg
     3961 tgcttcttta tcaatcactt cttgtaccgc acgcataact tctaaacata atcttgcacg
     4021 attttttaat gagtcggcac cgtaatggtc tgtacgtcta tttgaaaaag ttgagaaaaa
     4081 tgtttgaatc agcaaacgtt gtgcaatcga aatttccaca ccatcaaaac ctgctttaat
     4141 cgcgcgtaat gtagcatcgc gatactgctg aatgatgcta ttgattttct catgagacat
     4201 ggcgataaca acgtgttcaa tcggtgaatg caaagtcata ggacttggtc catacacctt
     4261 tccaaaattt aaaatggctt gatttgaaaa acgaccagca tgcgctagct ggataatagc
     4321 gaggctacca tgttgtttca tcgtagatgc catgttagtt aatccaggga tacaagcatc
     4381 atgatcaata ttaaagccat attcaaacaa tttaccataa ggttcaatgt aagcagcgcc
     4441 ggtgacttgc attccagctg aattagagcg acgtgcagca taagccaagt ctgcttttgt
     4501 aatatagcct tcttttgttg atgcgtttac ggtcattggt gataatacaa agcgattcga
     4561 aattttgatg ccattaggta agtggattga ttgtaaaagt ggtttgtatc ggtacatact
     4621 atgattcctt ttctattcaa tattgttttc aaagtaccat ggaaagaatg aataatcaat
     4681 gatgaacagt cttgatagaa tagaattggt acatggaaaa catttttaaa attgaactaa
     4741 tgaatggcat ttgtaggtct gaaaatatga atatgaaaaa gaaaaataaa ggcgaaaaga
     4801 tataaaagtt aattgaaaaa cgttatcata tacgtgggta tatgaagagg gaatggtatt
     4861 aagaacgcta aaatgttatg tcggtttgac atgacaggat aagtttggag atgacggatt
     4921 ggttaaatta agcgtattag actatgcctt aatagatgaa ggtaaggatg cacaaaaggc
     4981 attgcaagat tcagtgacac ttgcaaaatt agcagatcga cttggcttta agcgaatttg
     5041 gtttacggaa catcataatg taccagcgtt tgcgtgtagt agtccagaac ttttgatgat
     5101 gcatacattg gcgcagacaa atcacatacg agttggctct ggtggtgtga tgctgccgca
     5161 ctatcgacct tataaaattg ctgagcattt tagaatgatg gcagcgttat atccaaatcg
     5221 tattgattta ggtattggta ataatccagg tactactatg gtaaagcaag ctttagatgg
     5281 aataaatcct acatatgata gttacgatga atcgatttcg ttattacgtg attatcttac
     5341 aataaaggat aaaccaagtg cgcatacgtt aggtgtccaa ccacacattg atcattttcc
     5401 agaaatgtgg ttattaagta gtagcgcaac atctgccaaa atagctgccg aactaggtat
     5461 agggctttct gttggaacat ttttgctacc agatataaat gcgatacatg cagcgaagga
     5521 taacattgat atttacaaaa aacatttcca agcatcaacg attaaaatgg acgcaaaggt
     5581 gatggcatct gtatttgtca ttgtagcaga taacgaagcg gaagtagcag cattgcaaca
     5641 tgccttagat gtttggttat taggtaaatt acaatttgca gaatttgaac attttccttc
     5701 agtagacaca gcacaaaagt ataagcttaa tgatcgagac aaagagatga ttcaagtaca
     5761 tcaagcacgc atcattgcag gcacacaaga acaggttaaa gcacaattag atgatttcat
     5821 tgctacgttt gaagttgatg aggtgttagt agcaccgctt attccaggta ttgaacagcg
     5881 ttgtaaaaca ttaaaattac tcgcggaaat ttatttgtag cattttaaat agaagagaaa
     5941 ggatgaagat aagatgaaaa agttagccaa ttatttatgg gtagaaaaag taggagattt
     6001 gtatgtgttt agtatgacac ctgaattgca agatgatatt gggacagtag gttatgttga
     6061 attcgtaagt ccagatgaag ttaaagtgga tgatgaaatt gtgagtatcg aagcatcgaa
     6121 aacggtcatt gatgtgcaaa cgccattgtc aggaacgatt attgagcgaa atacaaaagc
     6181 ggaagaagaa ccgacaattt taaactctga aaaaccagaa gaaaattggt tgttcaaatt
     6241 ggatgatgtc gataaagaag cattcctagc attaccggag gcttaaatgg aaacgttaaa
     6301 atcaaataaa gcgagacttg aatatttaat caatgatatg cgtcgagaga gaaatgacaa
     6361 tgacgtattg gtaatgccat cttcatttga agatttgtgg gaattatatc gaggcttggc
     6421 aaatgtcaga ccggcattac ctgtaagtga tgaatattta gctgtacaag atgctatgtt
     6481 aagtgatttg aatcatcaac atgttacgga tttgaaggat ttgaagccga taaaaggtga
     6541 caatatcttt gtttggcaag gtgatatcac gacgttaaaa atcgatgcta ttgttaatgc
     6601 tgcaaatagt cgttttctag gatgtatgca agctaatcat gactgcattg ataatattat
     6661 tcatacaaaa gcgggtgttc aagttcgact tgattgtgca gagatcattc gacaacaagg
     6721 gcgcaatgaa ggtgtaggta aagccaaaaa aacacgtgga tataatttgc cagcaaagta
     6781 tataattcat acggttggtc cgcaaatacg tcgattgcct gtttcaaaga tgaatcagga
     6841 cttgttagct aaatgttatc ttagctgtct taaattggct gatcaacata gtttaaatca
     6901 tgtcgctttt tgctgtatat ctacaggtgt atttgctttt cctcaagatg aagcagcaga
     6961 aattgctgtt cgaacagtag aaagctatct caaagaaaca aattcaacat tgaaagtcgt
     7021 gttcaatgta tttacagata aggatttaca actgtataag gaggcattga accgtgatgc
     7081 agagtagtaa atggaatgca atgtctcttt taatggatga aaagacaaag caggctgaag
     7141 tattgcgtac tgcgattgat gaagcagatg cgatagtgat tggaattggt gcaggcatgt
     7201 ctgcatctga cggatttaca tatgtaggag agcgttttac ggaaaatttc ccagatttta
     7261 ttgaaaaata tcgcttcttt gatatgttgc aagcgagttt acatccttat ggcagttggc
     7321 aagagtattg ggcatttgag agtcgtttta ttacattaaa ttatttagat caacctgtag
     7381 gtcagtctta tctcgcatta aaatccttag tggaaggtaa acagtaccat atcataacta
     7441 cgaatgcaga caatgctttc gatgctgctg aatatgatat gactcatgta tttcatatac
     7501 aaggggagta tatactgcaa cagtgtagtc agcattgtca tgctcaaacg tatcgcaatg
     7561 atgatttaat tcgtaaaatg gttgttgcgc aacaagatat gcttatacct tgggagatga
     7621 ttccaagatg tccaaaatgt gatgctccaa tggaagtgaa taaacgtaaa gcggaagttg
     7681 gcatggttga agatgctgaa tttcatgcgc aactacaacg ttataatgct tttctagagc
     7741 aacatcaaga tgataaagtg ttgtatttgg aaattggaat tggttatact acaccacaat
     7801 ttgtgaagca tccttttcag cgtatgacac gtaaaaatga aaatgcaatt tatatgacga
     7861 tgaataaaaa ggcatatcgc attccgaatt caattcaaga acgtaccata catttaactg
     7921 aggatatctc aacattgatt acaacagcac tccggaacga cagtacaacg caaaataaca
     7981 acattggaga gacagaagat gtacttaata gaaccgatta gaaatggaga atatattact
     8041 gatggtgcga ttgcactggc tatgcaagtt tatgttaacc agcatatctt tttagatgaa
     8101 gatattttat tcccttatta ttgtgatcca aaagtggaaa ttggacgttt tcaaaatact
     8161 gctatagaag tgaatcaaga ttatatagat aaacacagta ttcaagtagt tcgccgagat
     8221 actggtggtg gcgctgtgta tgttgataaa ggtgccgtta atatgtgttg tattttagaa
     8281 caagacactt caatttatgg tgattttcaa cgattttatc aaccagctat aaaggcattg
     8341 catacattag gtgcaacaga tgtgatacaa agcggtagaa atgatttaac attgaacggc
     8401 aaaaaagtgt caggcgccgc aatgacatta atgaacaatc gtatttatgg cggttattcg
     8461 ctattacttg atgttaatta tgaagcaatg gataaagtgt taaagcctaa tcgcaaaaag
     8521 attgcatcga aagggattaa atctgtgcgc gcacgtgttg gtcatcttag agaagcactg
     8581 gatgaaaagt atcgtgatat aaccattgaa gaatttaaaa atttaatggt gacgcagatt
     8641 ttgggaatcg atgacattaa agaggcgaaa cgatatgaat taacggatgc agattgggaa
     8701 gcgattgatg aattagctga taaaaagtat aaaaattggg attggaatta tgggaagtca
     8761 cctaaatatg aatataatcg aagtgaaaga ttatcatcag gtactgtaga tattaccatt
     8821 tctgttgaac aaaatcgtat cgcagattgt cgtatttatg gggatttctt tggacaaggt
     8881 gatataaaag atgtggaaga agcattacaa ggaacaaaaa tgacaagaga agatttaatg
     8941 catcagttaa agcaattaga catcgtttat tattttggta atgttacggt agaatcattg
     9001 gttgagatga ttttaagtta atattgttta ggcgttaatg tcacaccctc aatgaatatg
     9061 tgtatttgct tttaaaatgg tgactatgta gttatgtaat ttgattatac gattgaaaat
     9121 gaatttagtt aattagcaaa gacatcatat ttatagcgtt tgtgtttatt agtgattggg
     9181 tatttgaaaa tggttaatga tagtttttaa aggaataaat tcattttcat ttatataagt
     9241 aagattaaat actagctact aaagtctatt tatatacgtc aaaatataat gactgctata
     9301 atgagtaatc aatagacaca aagaggagat tatgtgatga ataataaagt attagtaacc
     9361 ggtggtacag ggtttgttgg catgcgaatt atttcacgat tattagaaca aggttatgag
     9421 gtacaaacaa cgatacgtga tttaagtaaa gctgataaag taattaaaac aatgcaagac
     9481 aatggcattt ccacagagcg attaatgttt gtcgaagcgg atttatcaca agatgaacat
     9541 tgggatgaag caatgaaaga ttgtaaatat gtcttgagtg tagcatctcc ggtgtttttc
     9601 ggtaaaacag acgatgcaga agtgatggcg aagcctgcca ttgaaggcat acaacgtatt
     9661 ttaagagctg cagaacatgc tggcgtgaag cgtgtggtga tgactgcaaa ctttggtgca
     9721 gttggtttta gcaataaaga taaaaattca atcacaaatg aaagtcattg gacaaatgaa
     9781 gatgaaccag gcttatcagt atatgaaaaa tcaaaattgt tagctgaaaa ggcagcgtgg
     9841 gattttgttg agaatgaaaa tacaatagta gaatttgcca caatcaatcc agttgcaatt
     9901 tttggaccat cattagatgc acacgtttca ggaagtttcc acttgttaga aaacttatta
     9961 aatggttcaa tgaaacgtgt accgcaaatt ccattgaatg ttgttgatgt gagagacgta
    10021 gctgaactac acattttggc aatgacaaat gaacaggcta atggcaagcg atttattgct
    10081 acagctgatg gacaaattaa tttgttggaa attgcaaaat taattaaaga aaagagacct
    10141 gaaatagctc aaaaagtttc tactaaaaaa ttaccagact ttattttgag tctaggtgct
    10201 aaatttaatc atcaagctaa agaaggtaaa cttttattag atatgaaccg caatgtaagt
    10261 aacgaacgtg caaaaacatt acttggttgg gaaccgattg cgacacaaga agaagcaatt
    10321 ttagcagcaa ttgatagtat ggctaaattt catttaatat aatacaaaaa caccgtccat
    10381 aagtaagtgg ctttcctctt gataaaagag gcattcactc aatatgtgac ggtgttttat
    10441 atatttacgt taacactatt gctgtggctt ttgacggcct tttaataaaa ttgctgtcgc
    10501 acctacgata ataataaata gaatcgcacc aaataatccc atatatttta ctgcgttacc
    10561 gaacacgata ccgacagtta aaaagtctgt atctgagaat gttgttgcag caccacctaa
    10621 ttcgcctaaa aatggcaaga ataataatgg taaaaacgtg attaggatac catttagagc
    10681 ggcgccagca acagcacctt taataccgcc tcttgcatta ccgaatacag cagccgttgc
    10741 acctaagaag aagtgtgcaa ctacgccagg taaaatgacg acgccaccaa ataagaataa
    10801 gataaacata ccgatgacac ctgtaataaa gctgacaaag aatccaatta atactgcatt
    10861 ttgtgcataa gggaacacaa tagggcagtc taatgcaggt ttagaatttg gtacaagctt
    10921 ttcagaaatt cctttaaatg ctgggacgat ttcagctaag attaaacgaa cgcccgttaa
    10981 aataataaat acaccagcag caaatgtcac accttgaatt aaagaaaaga caataaagtt
    11041 ttgaccatca ctaatagatt cgtgtacata actaacgccc gcaaataagc atgcgatgaa
    11101 gtaaagtaat gccattgtaa tcgagatact aattgtactt tctcgtaaga aacttaagcc
    11161 ttttggaaat ttaatctctt ccgttgattt agacttacct ttgaataatt gacctacagc
    11221 acctgcggca aagtaactga ttgagccaaa atgacctaac gctacttggt cattccctgt
    11281 aatttttcgc atcgtaggtt ggagtaatgc aggtaatact gccatgatta atcctaatac
    11341 gagtgcgccg ataacaattg ttagccagcc tttaatatga ctgactgtta aaatgattgc
    11401 taaaaacgca gccatgtaaa atgtatgatg acctgttaaa aagatatatt ttaaattagt
    11461 gaagcgagca attaaaatat taacaatcat gccacagacc atgatgagtg cagctgttgt
    11521 tccaaaatct tttaaggcta gtgagacgat agcttcgttg ttaggtacga taccttgcac
    11581 accaaatgcg tgttggaata ttttgccgaa tggttcaaga gatcgaacga cgacatcagc
    11641 acctgcactt aaaattaaga agcctaatat cgttttaatg gttcctgaag tgatcgttgc
    11701 ggcaggtttt ttctgaacga ttaaacctat aaaggcaatc agtgcaacaa gaatggctgg
    11761 ttgacttaaa atatcgacta taaaattaag gattgcttgc ataggtcgta cctcctttaa
    11821 atcatgttaa gttgttgtaa tttttctgag agcttttgtt gtaattcagc tttatctaaa
    11881 atattatcaa gaactaagac atcccctagg cgttcggcat tttcagctaa atctctacca
    11941 caaataaaca agtcagccat ctctggactt gctgtcataa tgtcactatg ttcaacttcg
    12001 atatcagatg gtgcattaag ttgcctaagt gcttcttgtg cgttcatttc taccataaaa
    12061 ctacttccta aaccgtggcc acatactact aaaattttca tattaatcat gctcctttaa
    12121 aatgttttta atgtcttgtg cgttttttgc agttaatagt tgctggactg tttggttatc
    12181 gcccagtacg gttgctaaat tttgtaatac agataagtgt gaatgattgt cgatggcact
    12241 caatacaaaa atgagagatg cgtagtgatc ttcatcacaa aatgccacat gttgattcaa
    12301 ctttaataga cttaaaccaa cttgatgtac gtcattgttc ggtcttgcat gtgcaattgc
    12361 aatttcaggt gcgataacga tataaggtcc aagttcatta acgctatcaa tcattgcttg
    12421 aacatagcct tgttcaataa tttgttcttg tagtaatggc tgagaagcta tagttatagc
    12481 ttcagtccaa tcatttactt gttcttttac aatgatgcgt gttgttgaca aaatgtctaa
    12541 tgacacgtta ttaaacctcc tttgtcatag ttaaagcaat gtgttgttta attttaaaaa
    12601 tattcccatc taagaaatct tgtcgatata agtcgttgct taagcattcg cttaactgtc
    12661 ccaatgcctt taaatgtgca ttggggtggt ccgttgctaa tgtaattaca aggtgaacgg
    12721 gatcgttagc tttactacca aagataatcc cttcagtgaa atatgttagt gcgaaaccta
    12781 caccattctg tacataatca gtaccagcgt gaataagtgc aatatgtgga ctaatgacca
    12841 tatatgaccc gaattgttca aattgtttta aaattgcagc tgtataattt gaatagacaa
    12901 tgccatcatt gattaaaggt tgcacagcca ctgcaattgc ggattcaatt gataatggtt
    12961 gttcatttat aatgatgcga tgttcaggca ataaatctgc gagtgacttg ccatcagttg
    13021 ccattttcat gactcgttgt tctcttgagt cattgataat ttgattcaat ttttgacgag
    13081 attgttgatt gataaatgga tcgacatgaa taactggtac agctgatatt tcacaaggta
    13141 ctgttgaaat gacataatca atgttatctt gcaataatcg actttcttcc aattgataaa
    13201 tggaataggc atcccaaatg tgaaactcag gatacagatg atttagtttt gattttaaaa
    13261 gttgcgacgt gcctatacca gaaccacata gtaagacaac cttaatcatt gattgtttat
    13321 gtgttgcaac acgctctata cttgatgcga agtgaattgt aatgtatgtt aattcatctt
    13381 cgttgaagcg aatagcagca tcttgttcaa ttgggctaat atgcttgcta acggcttcaa
    13441 tgatttgagg atagcgacgc ataacttctt gcctcaaagg attaggttgt agcatatcgt
    13501 atttaatacg atgtatagct ggtttgatat gtgtgatcag actggtatgc aacttgttgt
    13561 cttttgacat atcaatgcct aattcttggc taacacaagt gatcaattca tgtatatttt
    13621 gcgataaatc atggtattca aaggtaattg aagatgctgt atgttcggtc attttagagc
    13681 ctagtaaatg taacgtgata aagataattt cagactctgg aaatgtgaca ttacaactgc
    13741 gttctaagtt ttctatcatt tttgaagcaa tagcatactg attagtatgt cgccatttat
    13801 caatttcatt gataggtata tcgaacgaaa aattttcatt taaacgctga atggcaatga
    13861 gtatatgata gattaagcca tcgatagccg actgaactaa atgataattt tcactattta
    13921 atgtcttaat aatggcacgg cgaaccaatg cgattgattc tgaattaaag atatccgcct
    13981 ctataaaagg tgcagcttgt ttcatatatt gatgtataaa gtgtgcatac gctttacgat
    14041 aatgatcttc ctcaccaata acattgaatc ctttattgtg gacataattt aactttaaat
    14101 ggtattgatc tagttgggct tgaatcattt taatatcatc tgcaattgtc cgacgcgaaa
    14161 cattaacatc ttgcgcaagt tgctttgttg aaacaggatc ggttgtttcg aataacttta
    14221 aagcgatatg tgtgagtcgt tcatcttttg aaaaatgaat ttgatttgtt agattgtgct
    14281 ctaattcatt taatagtgtc gcgtgagctg ttgttacttt gatgcctgca gctttattac
    14341 ggctgacttg gtaatgataa gtttcagcat attgctcaat atatgctata tcatattgaa
    14401 tggtacgagg tgatacacca agttgattag caatggtatt gattggaata aacgtttgct
    14461 catgaattaa aagatacaaa atttcgattt gtctataact taacaacgta atatcctcct
    14521 atttgtaatt gtaagcgatt tcttaaaaac gtagatatgc aatctctttc atattttaat
    14581 ccgaaaaatt gcatatcaaa atgtttatgg cgcaagattt tataggaact tttaaaataa
    14641 attagatatt catgttgaca atttaaaaat gtcgcagtat atttagttag acatctaacg
    14701 aaatggtggt gcaataaatg gaattcactt attcgtattt atttagaatg attagtcatg
    14761 agatgaaaca aaaggctgat caaaagttag agcaatttga tattacaaat gagcaaggtc
    14821 atacgttagg ttatctttat gcacatcaac aagatggact gacacaaaat gatattgcta
    14881 aagcattaca acgaacaggt ccaactgtca gtaatttatt aaggaacctt gaacgtaaaa
    14941 agctgatcta tcgctatgtc gatgcacaag atacgagaag aaagaatata ggactgacta
    15001 cctctgggat taaacttgta gaagcattca cttcgatatt tgatgaaatg gagcaaacac
    15061 tcgtatcgca gttatctgaa gaagaaaatg aacaaatgaa agcaaactta actaaaatgt
    15121 tatctagttt acaataaatg gtaagtgtga ctggtagaaa tcagtcactt tatctttaat
    15181 attatagtta gatatctaat tgttagtaag ctaattattg gaaaagacaa ggagtattga
    15241 acaatgaaag acgaacaatt atattatttt gagaaatcgc cagtatttaa agcgatgatg
    15301 catttctcat tgccaatgat gatagggact ttattaagcg ttatttatgg catattaaat
    15361 atttacttta taggattttt agaagatagc cacatgattt ctgctatctc tctaacactg
    15421 ccagtatttg ctatcttaat ggggttgggt aatttatttg gcgttggtgc aggaacttat
    15481 atttcacgtt tattaggtgc gaaagactat agtaagagta aatttgtaag tagtttctct
    15541 atttatggtg gtattgcact aggacttatc gtgattttag ttactttacc attcagtgat
    15601 caaatcgcag caattttagg agcgagaggt gaaacgttag ctttaacaag taattatttg
    15661 aaagtaatgt ttttaagtgc accttttgta attttgttct tcatattaga acaatttgca
    15721 cgtgcaattg gggcaccaat gatttctatg attggtatgt tagctagtgt aggcttaaat
    15781 attattttag atccaatttt aatttttggt tttgatttaa acgttgttgg tgcagctttg
    15841 ggtacagcaa tcagtaatgt tgctgctgct ctgttcttta tcatttattt tatgaaaaat
    15901 agtgacgttg tgtcagttaa tattaaactt gcgaaaccta ataaagaaat gctttctgaa
    15961 atctttaaaa taggtattcc tgcattttta atgagtatct taatgggatt cacaggatta
    16021 gttttaaatt tatttttagc acattatgga aacttcgcga ttgcaagtta tggtatctca
    16081 tttagacttg tgcaatttcc agaacttatt atcatgggat tatgtgaagg tgttgtacca
    16141 ctaattgcat ataactttat ggcaaataaa ggccgtatga aagacgttat caaagcagtt
    16201 atcatgtcta tcggcgttat ctttgttgta tgtatgattg ctgtatttac aattggacat
    16261 catatggtcg gactatttac tactgatcaa gccattgttg agatggcgac atttattttg
    16321 aaagtaacaa tggcatcatt attattaaat ggtataggtt tcttgtttac tggtatgctt
    16381 caagcgactg ggcaaggtcg tggtgctaca attatggcca ttttacaagg tgcgattatc
    16441 attccagtat tatttattat gaatgctttg tttggactaa caggtgtcat ttggtcatta
    16501 ttaattgctg agtcactttg tgctttagca gcaatgttaa tcgtttattt attacgtgat
    16561 cgtttgacag ttgatacatc tgaattaata gaaggttaaa tatttcgtac acttctggct
    16621 gagtatattt cggtcggaag ggtatttttc gaaaaaaata aatatatgat acgattatga
    16681 aaaaataaag tgagattggc atatgtacaa atctaaaata ctgttgaaat atatttttag
    16741 tgaagaatca gaagttaaag atttaactga agaaaaatat aatcaagatt acgaagcatt
    16801 aacatttagc tttaaagagg aaacatatca aagtaggtta gctaagaaaa caccgactaa
    16861 agcagggtat tttgttacat gttggacgaa agacgagaac aattgtaatc aaccatactc
    16921 gaaagaggca tttgcagatt acttaatgat tatagttatt gatgaagaat taagcggtta
    16981 ttttttattt cctagggaat tattggtaga aaaaggtatc ttaactacat ttgaacataa
    17041 aggtaagatg gcttttagag tttatcctaa gtggtgtaat caattgaata aaacagcagg
    17101 gcaaacacaa aagtggcaat gtaaatattt ttttgaatac taataaaaag tcatatggtt
    17161 gaacttataa gagatgaata tatcatcttt cgataagata accatatgac tttatttttt
    17221 atcatttaat gatgaacggt ttcttgtcct actttattcc aagtgaggat aaagctcaac
    17281 attgcaaaca cactgattgc tgttaataaa ataaaaccga catcccatcc gaatttatca
    17341 actacagcac ctaagacgat gttggccatt acagcaccaa acagataacc aaataatcct
    17401 gttaatccag ctgctgtgcc agctgctttt ttaggtacat aatctaatgc ttgtaaacca
    17461 attaacataa ctggtccata tattaagaaa ccaatggcaa ttaatgagac attgtctaac
    17521 caagcattgc ctggaggatt taaccaataa attaatacaa atactgtgac acctaacata
    17581 aagaagaaac ctgcaggtcc acgacgacct ttgaataatt tatcagaaat gtaaccacat
    17641 aataatgtac caggaattcc agcccattcg tataagaagt atgcccaacc tgatgctttt
    17701 aagtcgaaat gtttttcttc acttaagtag actggcgccc aatcaagtac accataacgc
    17761 acgaaataaa caaatatatt tgcaaaggca attgcccata cccatttatt gttcagtaca
    17821 tatttaaata aaatttcttt tgtagttaat tctgtttcta atgttttctt atcgcttgta
    17881 gcaaagtcgt ttttataaat ttcgattgga ggtaaacctt gagattgagg tgtgtctcta
    17941 atcaatacgt atgaaattgc ggcaatgata agtgctaaga gtgcagggta aatgaataca
    18001 ccttcgaaac cttttaaata accaaagttg ataaatgctg ttgttgtaat accccaagca
    18061 gcaataggtg ccataatacc tccaccaaca ttatgcgcaa cgttccaaag ggcagtctta
    18121 cttccgcgtt cacttacact aaaccagtga acgagaacac ggcctgaagg tggccagccc
    18181 ataccttgaa accatccatt taagaataat aggacaaaca taataccgat acctgatgta
    18241 aagaacggta caaatcccat taacaaattg acgatagcag tgagtgctaa tccaagaact
    18301 aagaatatcc gagcattgct ccgatcactt acagtaccca taaagaactt actaaatcca
    18361 tatgcgatgg aaacagcaga aagtgcaaaa cctagttccg cttttgtaaa accttgctct
    18421 tgcaatgctg gcatcgctaa tgaaaagttt ttacgtaata aatagtaccc agcgtaaccg
    18481 atgaaaatac caagaaatac ttggagacgt aatcgtttat aggtatcatc tatctgattt
    18541 tctggcaaag gcttaatatg ctttgcaggt ttaagaaaat tcataaaatc ctccttaata
    18601 tgtatttata tgcattttgt gcgataaact tatattagac atgtatgaaa gcgttgtcaa
    18661 tatacttttg tgaaatcttg catatttagt tattgaagta ttttataaac atttagaaaa
    18721 aataaattgc tacacataaa tttataatta tgcaattatt atgtcgttat acatactagc
    18781 tgtgtatcta cgaatgagta caaacatatt tttatttgca gaaaaggggt aaatggcata
    18841 taactatctt ttttatgtaa gttggtataa aatttatact aataggaggg atagtatgaa
    18901 tatagtaggg catcatcaca tatccatgta tacaaaagat gcaaaacgta ataaggattt
    18961 ttacacaaat gtccttggat tacgattagt tgaaaagtct gttaatcaag acaatccttc
    19021 aatgtatcat ttgttttatg gggacgaagt aggtacagcc ggaacaattt taagcttttt
    19081 tgaaattccc catgcgggtc ataagcagcc aggtactgaa acgatttatc gattttcttt
    19141 attagtacca aatcaagcgg cacttcatta ttttgaaaaa cgccttgata ataatggtat
    19201 tacgtctgaa cgtttgtact atcttggaca agaaggtgtt gtctttaaag atgaagacga
    19261 cttagaaatc atattgcttg ttaatgatag ttttgaagta ccacatcaat ggcaacataa
    19321 cgcttatagt gaaatacctc aagcatatca aattttagga atagggccag tcgaattaag
    19381 agttagaaat gcagcgcgta cggtagaatt tttggaaaat gtcttaggtt atcgcaaaag
    19441 agataataaa tcattcgatg tgctgacatt agcaccacaa ggtttatatt cggattttgt
    19501 agttattgag caacagggac aacgtgaaag acctggacga ggttatatcc atcatattgc
    19561 aattaataca ccacaaatga gtgacttaga tgcaatttac aagaaattac aacaacaaac
    19621 acaaagtaat tcaggtataa ttgatcgcta tttctttaaa tcattatact atcgccataa
    19681 ttcaattatg tatgaatttg cgactgaagc gcctggattt actattgata cacctcttga
    19741 acaattagga agtcaattga acttgcctga ctttttagaa gcagaacgtg aacaaattga
    19801 aagtaagtta cacgaaatat aaaggagaat gtttaatggc caaattagaa atgaataaaa
    19861 atacgcctct tgagtttggt ttgtattcct taggtgatca tttattgaat ccatttaaag
    19921 gtgaaaaagt tagttatgag caacgtatta atgaaattat tgaagcaagt aaattagcag
    19981 atgaaacagg tattgatgtt tttgcagttg gtgaaagtca ccaggagcat tttacaacac
    20041 aggcacatac ggttgtgtta ggtgcaattg cccaagcgac aaagcatatt aaagtttcaa
    20101 gttcttcaac gattattagt gcaacagatc ctgtaagagt atttgaagac ttcgcgacat
    20161 tagatttgat ttctcatggt agagccgaaa ttgtagctgg cagagcatca agaacaggta
    20221 tttttgactt gtttggctat gatttaaaag actatgatga attgtttgaa gaaaaattag
    20281 gtttactttt agagttaaat aaaactgagc gtattacttg gtctggaaaa tatcgtccag
    20341 aacttagaaa tatgaaaata ttcccaagac caatcgataa tatattgcca atatggcgtg
    20401 ctgttggtgg tccacctgca agtgctatta aagcgggaaa acaaggtgtg ccaatgatga
    20461 ttacaaccct tggtggccca gcaatgaact ttaaaggttc tatagatgct tatcgtcaag
    20521 cagcaactga agcaggtttc gatgcttcgc ataagtcttt accagtaagt acagcgagtc
    20581 tgttttatac agctgaaaca actcaggatg ctatgagaga attttatcca catttgaata
    20641 cagggatgtc atttattcgt ggtgttggtt atccgaaaca gcaatttgct aattcgtcag
    20701 attatcgaga agcgctaatg gttggaagcc cgcaacaaat tattgaaaag atattgtatc
    20761 aacacgagtt gtatggtcat caacgtttta tggcacagct tgattttggc ggtgtgccat
    20821 ttgaaaatgt tatgaagaat attgagttaa ttggcaacga aattataccg gcgattaaaa
    20881 agcatttatc aaaataggag gggcgtcatc atgaatattg tattattgtc aggttccaca
    20941 gtaggttcta aaacgagaat tgctatggat gatttaaaaa atgaactaga agtcatcaat
    21001 gagggacatc aaatagagtt gatggattta cgagaacttg aattagaatt tagcgttgga
    21061 aagaattatc tagatactac aggagatgta tataaattaa cgacgtcgtt aatgcaggct
    21121 gatgtgattt ttattggttt tccaattttt caagcttcca tccctggtgc tttgaaaaat
    21181 gtgtttgatc tacttccagt caatgctttt cgtgacaagg taataggact tgtagcgaca
    21241 gcaggttcta gtaaacatta tttaattcct gaaatgcatt taaaaccaat attgagttac
    21301 atgaaagcac atacgatgca aacgtatgta tttattgaag agaaagattt ttcaaatcaa
    21361 caaattgtca atgatgatgt tgtatttcgg ttaaaagcgt tggcacaatt cacaatgcga
    21421 actgccaaag tacaacaaca agtgtttgaa gaagaaaaca accaatacga cttttaaagt
    21481 ataaaaataa gacgctcggc acactaaatt tgtatgtgtt tgagcgtctt ttatattaac
    21541 tatatagcca gtgaacgacg ataaaggcaa gtgatgacaa gcatattgag gtaataatga
    21601 ttgtcataag cggtttaagt gcacgatttt taagatcttt aaatgcaaca tttaacccta
    21661 aagcaaccat ggccattaat aagcaaattg ttgatatagt atttaaaata tttaacaata
    21721 ctgatggaat agttacatat gtattcacta aggccataat gacaaatcca attaaaaagt
    21781 atggaatgct tattcgaccc ttgctagatg attctgatga acggaaacgc ataattaaaa
    21841 taagtacgat ggttaatgga atcagtaaga atactctacc aagtttacca agaagtgcaa
    21901 ttttaagtgc atcactacca ccaaagccac cagctaagac aacgtgcgca atttcatgaa
    21961 gactaacacc agaccaagcg ccataaacat ttgtcgtcat tgaaaagata gcgtagatag
    22021 ctgtatatat aagtgagaat atcgtaccaa tcaatgcgat gataccgata ctaatagctg
    22081 tatccttttc acgtgatttg aatattggag cgactgcggc aatagcggca gcaccacaaa
    22141 cgcctgtgcc gacacctagt aataatgcga tgtttttgtc accatgcaac agtttgttga
    22201 caaagagcat cattacaata ctgaaaataa cgacacctac atcgatggct aatagtttac
    22261 taccttgacc gataatatcg aatatattga gtttaagtcc atataggatg attgcaaatc
    22321 ttaataaata tttagatgaa aacgtaatac ctgagctata ttgttcagga tatcctctaa
    22381 agtgacgata tagaatagcg attaatatcg cgatagttaa tgcgccaacc ttatctaaga
    22441 ttggcaattt agctgctaaa aagctaaata atgcgactat aaatgttaat gatagtccaa
    22501 tcataaaatg cttatttttc aatgatgcca tgagcagtgc ctcctttaat agcattttag
    22561 cactgttttg tcgtattttt aaatataaat ttggaatggt taataaagta gtgattaaat
    22621 taagttgtgt gataggtatc aaggacatca accaaagtaa taggcactac aacgcttatt
    22681 ggcggggccc caacaaagaa gctgacgaaa agtcagctta caataatgtg caagttgggg
    22741 atgggcccca acatagagaa attgggtccg taatttctac agacaatgca agttggcggg
    22801 gccccaacaa atttaagata caatgcgtaa tattaatatg ttattattat gataatttac
    22861 agaattatat gaaaaatgaa tgaggatgtg atggtatgtt tggaatgaaa gtgaatgaac
    22921 aaataacatt aaaaatttta gaagctcatg acacagaagc gcttttcaat ttagtcaatc
    22981 gttcaagaaa ttcacttagg gaatggttac cttgggtaga tgcaactgag caaccatcag
    23041 atacgcgtgc atttattaaa agaggacttt tgcaatttgc tgatggtaat ggatttcagt
    23101 gtggcatttg gtatgaagga acgctagttg gtgtcatcgg tttacatgaa attaatcaca
    23161 tgcacagaaa aacttcatta gggtactatt tagataaaca atttgagggt catgggatta
    23221 tgacacaagc agttgaggca ttgataaagt attgtttcga agagcttgac ttaaaccgaa
    23281 ttgagattag tgccgcagtt aataatgaaa aaagccgagc tattcctgaa aggctgggat
    23341 ttactagaga aggtatgtta cgtgacaatg aattactaaa tggtatttat tcatcgagtt
    23401 acatctatag tttattaaaa tcagaatacg accaaaaatg acaaattaga cttacaaaag
    23461 agtgatgaca tttaaaatgg catcgctctt ttatttaatt tttgaaaata aaaggttgtt
    23521 gacagtatta ttttataaca atataatgat tttgataatt attatcaact agataatgtt
    23581 catgggagga tgccttaaaa tcatcatttt aagagccacg tattatcttg gcatttaggg
    23641 agtgtgagat agtacgaatg agggcatgtc tatggggata atagaatttc tataatgagg
    23701 tgtcaaaatg aaaaagttaa caacgctatt attagcatca acgttattaa ttgctgcatg
    23761 tgggaacgac gatagtaaga aggatgattc aaagacatcg aaaaaagatg atggtgttaa
    23821 agcagaatta aaacaagcaa caaaagcata tgataaatat actgatgaac agttaaatga
    23881 atttttaaaa ggtacagaaa aatttgttaa agcgattgaa aataatgata tggcccaagc
    23941 aaaagcgtta tatccaaaag ttcgtatgta ttatgaacgc tctgaaccag ttgcagaagc
    24001 atttggagat ttagatccta aaattgatgc acgtcttgca gatatgaaag aagagaaaaa
    24061 ggaaaaagaa tggtcaggat atcataagat tgaaaaagca ttatacgaag ataagaaaat
    24121 tgatgatgtg actaaaaaag atgcacaaca attattgaaa gatgcaaaag aattgcatgc
    24181 caaagctgat acattagata tcacaccaaa attaatgtta caaggttctg ttgacctatt
    24241 aaatgaagtt gcaacttcta aaatcacagg tgaagaagaa atttattcac atacagattt
    24301 atatgatttt aaagcgaacg ttgaaggcgc acaaaaaatt tatgacttat ttaaacctat
    24361 tttagagaaa aaagataaaa aattaagtga tgatattcaa atgaacttcg ataaagtgaa
    24421 tcaattattg gataaatata aagataacaa cggcggttat gagtcatttg aaaaagtatc
    24481 gaagaaagac cgtaaagcat ttgcggatgc tgttaatgca ttaggagagc cactaagtaa
    24541 aatggctgtg attactgaat gacaaattat gaacaagtta acgatagtac gcaattttca
    24601 agacgtacat ttttgaaaat gttaggtatt ggcggtgccg gtgttgcaat tggcgcaagt
    24661 ggtgttggta gcatgtggtc tttcaaatca atgttcaata caccagaaga tccggaaaaa
    24721 gatgcgtatg aattttatgg taaagtgcaa ccaggcatta ccacacccac gcaaaaaaca
    24781 tgcaatttcg ttgcgttaga tttgaagtca aaagatagag atgcaattaa ggcaatgttt
    24841 aaaaagtgga cggttatggc tgatcgtatg atggatggtg atacagttgg caagacgagt
    24901 aacaatcctt taatgccacc agtagatacc ggtgaatcga taggattagg tgcaagcaag
    24961 ttaacgatta cctttgggat tagtaagtct ttgatgaaga aaattgggtt atctagtaaa
    25021 attcccgatg cctttaaaga tttaccgcat tttccgaatg atcagttaat agacgattac
    25081 agcgatggtg atattatgat tcaagcatgc tcaaatgatt cgcaagtatc ctttcatgcg
    25141 gttcataatt tagttcgtcc atttcgagat attgttaagg tacgttgggc gcaatctggt
    25201 tttatctctg ctaaaggtaa ggaaacacct agaaatttaa tggcatttaa agatggaaca
    25261 attaatccta gaaagagtaa tcaacttaaa gattatgtgt ttattgatga cggatgggcg
    25321 aaacatggaa cttattgtgt tgtcagacgt attcaaatac acattgaaac gtgggatcgt
    25381 actgcactgg aagaacaaga ggctacattt ggtcggaaac gacatagtgg tgcgccgtta
    25441 acaggtggga aagagtttga tgaaattgac ttaaaagcga aagatagtca tggcgagtat
    25501 attattgata aagatgccca tacgagacta gcgaaagaag caaatacgtc aattttacgt
    25561 agagccttta attatgttga tggtacggat gaccgcacag gtaacttcga aacaggcttg
    25621 ttgttcattg cttttcaaaa agcgacaaaa caatttatcg atatacaaaa taatttaggt
    25681 agtaatgata aattaaatga atatattaca catagaggtt ctgcttcatt tttagtatta
    25741 ccaggtgtta gtaagggagg ataccttggt gaaacattat ttgactaaat ttgtagcaat
    25801 gctaataact gctgctatgg tgtgtagctt tgggttactg aaaagtcagg cagcagaaca
    25861 acaaagtatt agtgatgtat atagtgtgat aacggatgcg aaatctgcac tttctaataa
    25921 ttcgatatcg aatgacaata agcagaaagc aattgagcaa gtggtaagtg cagttaagaa
    25981 attatcgctt gaagataata gtgaaggtaa tgctgtcaaa tcagatgtga gaaagcttga
    26041 agatgcaaaa gcgaatgata atcaaaaaga tacactttcg caattaacga agtcattaat
    26101 tgcttatgaa gagaaattgg ctagtaaaga tgcgggttct aaaattaaac tattgcaaca
    26161 gcaagtcgat gccaaagatg ctgcgatgac aaaagcgatt aaagataaaa ataaagcgga
    26221 attagaatct ttgaacaata gtttgaatca gatttggaca agtaatgaaa cagtgattcg
    26281 caattatgac gcaaatcaat atggacaaat tgaagtcgca ttattacaac ttagaattgc
    26341 aattcataag tcaccattag atacggcaaa agtgtcacat gcttggacaa cttttaaatc
    26401 aaatattgat catgtcgata aaaaaagtga tacgtctgca aatgatcaat accatgtatc
    26461 acaattaaat gatgcgttag agaaggcgat taaagctatc gacgacaatc aattgtcgga
    26521 tgctgatgct gcgcttacac attttataga aatttggccg tatgttgaag gtcaaattca
    26581 aactaaagac ggggctttgt atacgaaaat tgaagataaa ataccatatt atcaaagtgt
    26641 attagacgaa cataataaat cgcatgtcaa agatggttta gtagatataa acaatcaaat
    26701 taaagaggtt gttggccata gttatagctt tgtcgatgtg atgattatct ttttacgtga
    26761 aggactagaa gtgttattaa ttgtaatgac attgactacc atgacgcgta aagtcaaaga
    26821 taataagggg actgcaagtg tgattggtgg tgcaattgcc ggacttgtac tgagtattat
    26881 cttagcaatt acgtttgtag aaactttagg gaatagtggc attcttcgtg aaagtatgga
    26941 agcgggatta ggtatcgttg cggtcatatt aatgtttatc gttggtgttt ggatgcacaa
    27001 acgttcaaat gcgaaacgct ggaatgatat gattaaaaat atgtatgcca atgcgattag
    27061 taatggtaat ttggtattgt tagcgacgat tggtttaata tctgtgttgc gtgaaggtgt
    27121 tgaggttatc attttctata tggggatgat aggtgagcta gcgacaaaag attttattat
    27181 tggtattgcc ttggccatag tgatattaat tgtctttgcg gtattattta gatttatcgt
    27241 cagattgata cctatattct atatatttag agtattgtcg atctttattt ttattatggg
    27301 attcaaaatg cttggcgtaa gtattcaaaa gttacaatta ttaggtgcga tgccaagaca
    27361 tgttattgaa ggattcccaa cgattaactg gttgggcttc tatccaagtt atgaaccatt
    27421 gatagcacaa gctgcttata ttatggtagt tgctatctta atctttaaat ttaaaaaata
    27481 aaaaacaggc cgagtgcctg tttttttgtt gctgtattgc ggattatcgt agcattgttg
    27541 agatgtggat atcgctgcat ggttggttct cgttgtattg cagatatcac ggtttaatcc
    27601 gattctgtat tctaattaac gcaagtgcag tattgatata atctgaggtg ctacattgtt
    27661 cttttattgg ctattgtgat gttgctgcat tgtgagtatc acagcattaa ttcgtataag
    27721 gttaatgtgt tggcggtttg cctcggcatg tgaacttaac aatgaacata ctgaactcaa
    27781 agagcaatat gagtggcaat gtaagtaata tatttaatgt taaatcgggt ggtgcaatga
    27841 tacttgccag cacaaagcaa gcgaaataaa tatattttcg ataatgcttc aatgatgtga
    27901 tatctataag accgaatttt gcaagtccta tgaataatat tggcaattgg aataaaatgc
    27961 caaaagtgaa aagccaacgg ataagttcaa ctaaatacgc tttaaagcca atgacaggcg
    28021 aaatgtttaa agttaatgat aattttaacg cgaattgaat gattattgga aagccaacat
    28081 aaaatgcaaa agcgacgcca gcacagaata ataacacgct gaaaaaacta tatttataaa
    28141 taaattgacg ttcattatta tgtaatccag gtgcaatgaa tgcccacaat tgataaaaca
    28201 tgactggtga aatgaaacaa aacgcgatga aaaatataat catcacgtat atttggatca
    28261 tttctgtgaa tgaaaatgca tgtaaggaca catgtgcacg ggtaatatac gttatgaatg
    28321 gtgtcatcca ccaaaatgat gaaacatata cgacgatgac tgtgacgacg aacgacaata
    28381 gtattttaac taaacgatga cgtagctcgc taaaatgaac tagtaaggtg tggtcagtgc
    28441 tattgctctc gctgttgttt cgattcctta ctgggtgtgt cgtgagactc tttgtctaaa
    28501 tcttctgttg cagatttaaa ttcttttaaa gtagaaccga tagcacgacc aaattgtggt
    28561 aattttttcg gaccaaaaat aattaaagcg ataatgctaa tgacgacaag acttgttggg
    28621 cctgtgatgc ctaaaataaa agtgttagtt atcatgataa tcaacctcac tcataagtag
    28681 tatatagtat ttactttata ccgaacgata atgattatca ataaaatttt ataaaataat
    28741 taaaatgttg ctgtaaatac aaattactga ggtattaatt ctgaaataat agcttgttct
    28801 ttcaagtcga tataagtaca agtaccagct tgaatatcga tatgccattt ataaatgcca
    28861 gcttcagcca tttcatcaca aaatgtttcg aaatctgttt gtccttgttg atgtctagtt
    28921 aatacatctt gaactattgt tttgtttgat ttttgagcaa caggatgatt acttttcaca
    28981 gatgacgtaa cgatatcatc ttctgattga tgtacgtatg ttgcagtgcc atcttgaatg
    29041 ttgacgatat tgtaagtcat ccccatatct ttaaaagctt tgaatagttt tggaaagtca
    29101 acaccagtaa attgttgatg tgcttgttga attgcagata atgtaaatgc cataatagtc
    29161 tcctttgaat ggtttgattt agtgaaaatg cgtgcatcat actaaaatta gaatgctgtt
    29221 aattatgcag gaaggtgtaa cgaaagtaaa caattatgac ttgtaggcgt tgaggtgttg
    29281 tttcaaaatg tcatacatag gtggacaatc atattttttt gtgtagattt gacaaatata
    29341 gttgtcaaaa agacaaatat catttacatt aggactaagg agttgatgcg atgtgcgtaa
    29401 tcgattgaaa gagttacgag cacgagatgg cttaaatcaa acgcagcttg ccaaactagc
    29461 tggtgtttca agacaaacca tttcgctaat tgagcgaaac aattttatgc catcagtatt
    29521 aacggcaata aagattgctc gcattttcaa tgaaacggtg gaaacggttt ttattattga
    29581 ggaggatgaa gcatgaaaat actaagatat atcggatatc ttttactggg tggaattgta
    29641 gggggtatca ttggtggaat tttaggtaac tttgatggat tgggtattga gaacttgacg
    29701 tttgcgactt ataacaatgt cgttgtaata tcgattgttg cgactatgat catcatattg
    29761 gtagaagcca ttgttttgat gaatcaaaga cgtgcattga agtataagcg acttgtagat
    29821 gaagaggtag atatcgatgc aacagatcaa tatgaattgc ttgcgaatcg ttatgtttta
    29881 aatggaagta tattaagcgt tatacagaca attattgcct ttgtagtgtt actaattttt
    29941 gtggtagggc aagctgaagc aaatgcaatg ctattctttt taataccatt ttttgctagt
    30001 gctattttca atacacaatt tacactgttt aatagaaaat ttgatgacag aatgccaaaa
    30061 attgcagata agaattacac tgaaaagcga ttggaaatat tggatgaagg tgaacgccat
    30121 atagaattaa ttgcattatt taaaacatat gcgatcaact tatcaatatt gatactagcc
    30181 attattttta tagggtctta ttcaattgct acaggaatta atcaaagctt tagtttgcta
    30241 cttatcattg ctattttcat atataacgcc tttagctatt tattgaagag aagacgtttt
    30301 tattaaaatt aaacaaagag gagcatggat atgacaacat tgttaaacgt agatcgtgtg
    30361 aacaaacaat acaaagattc ggattttaaa ttgcaagatg catctttaac gatttctact
    30421 aatgagacag ttggattaat tgggaaaaat ggctcaggta aatcgacatt aattaatatt
    30481 ctagtaggca atcgacataa agataacggt agtattgcat tttttggaga agaacatgct
    30541 gcggatgatg tcgaatataa agaacacata ggtgttgtgt ttgatgatct gagagtacct
    30601 aataaattga ctattaaaga tattgataaa gtatttcaat ctatttatac gacttggaat
    30661 agtcaaaaat tctttgattt aatcaaatat ttcgagttac cactacaaac taaaattaaa
    30721 actttttcaa gagggatgcg aatgaagata gctttaacga ttgcgctttc tcatgatgtg
    30781 aagttattaa tcttagatga agcaactgca ggtatggatg tttctggaag agaagaagta
    30841 atggaactat tagaagattt tgtcgctcaa ggtggaggca tcttaatatc atcgcatatt
    30901 tctgaagata tagaacaatt agcggataaa ttagtgttta tgaaagatgg acgaatgatt
    30961 ttaactgaac agaaagatat acttttagca caatatggaa ttgttacgac aggagataaa
    31021 gatgttgaaa ttcctaagca tttaatcatt gcttctagat tgtcaaaggg gaaatatcaa
    31081 attttagtta aagattatgc agaaattgaa aatgcagagc ctttaaaaca cattgatgac
    31141 gctacgaaaa tcataatgcg aggtgaagta taatgaaagg tatgttccta agtagttttt
    31201 atgcaacgag aaagcaaaca tatatttatt ttatagtcgc tatcatagct gcgggatact
    31261 ttgcagtatt taatccgttg atgagttcgg caatggctgg ggttatgtta atcacaccca
    31321 ttactgataa tattaaacat gaaaaagact caagatggat gtattatgta tctactttac
    31381 cggttaaacg tagtgattat attaagtcat actttgcctt ttatttaatc ttattcggtg
    31441 caagtttaat gattggatta gttgtgacta cgatcgtgac ccaaagtgtg atgattggta
    31501 ttatgtcagg tttaatgagt tttggtatca taggggcata ctctatcatt ttcccattga
    31561 catttaaatt tggcgctgaa aactctaatg tcattatgat agttgcatct atcctactac
    31621 ttttttcttt cgtggtcttt ttctttatat atggtatggt tagtggtgca tcggcattag
    31681 aatttgaaaa aattagcact gaaggatggt tagttgtcat agtatatgcg gtcattggta
    31741 tcgttataac aagtgtttct tatatattgt ctattaaaat ttttaacaaa caagaactat
    31801 aatcaatatt cttggtgtga agctagtcat tgaaataata tttagagcag gtgataaatc
    31861 aatacgatta tcatctgctc tttttggtgt tgtggaatga aatgcgggat gataggttga
    31921 ggtgacatct ctatatcata tcatttgttt ttagaagatt aggttatgtg ggaattgcat
    31981 ccttttatag tgaggattaa cgtgtatata tcattgagta tgttgctgaa atttatttgc
    32041 gtgtatcatt tgatgattgt ataggctaac gatttccttg gaaatattta aaaacctcga
    32101 tcatgtagca taactgaatc caaaagtata atgtgaagtt cgacactttt ggattcagtt
    32161 caaatacttt gaccgaggtg aatactattt attcatgttt attacgtaga cgttgatttt
    32221 taaaataaat cagcatgatg cagaaagtcg caacggatac cgagataatc attacttggt
    32281 catgcccctt aaagataagg ctgatgatat atgatactac aagtatgcct agtgaagaag
    32341 aaatatattt tgcgtttgtc agttcattat ggaaataagg cgtgattaac cataatccaa
    32401 tatagaatat taaaacactg atatacatca tattaatttc aaacaagtca tttagtttat
    32461 tgttattact aaaaacaatt gcagcattaa tcacacctaa agcgatattg attaatagat
    32521 gcgtatacga taaacggaaa ccgatagatg acaatttatg attaatgtaa ttttcagtaa
    32581 tgatccaata tacaccgaaa agactaatta aaatcataaa ttggaatata taaatgtaac
    32641 taaaatgatc aatgctaaat gatgacgaag ctaaaccaac cagtacctcg ccaaagataa
    32701 taattgttag taacgaaaaa cgttctacta aatgcatcat attaacaggt gataatacaa
    32761 gatatttctg aaatggaata agtcctgtcg ctgcaatgaa tacgcctaaa aatccaggga
    32821 tgtaatggat actttgtggt agtactaatg atagaaatga taaaaatgaa atcacaaagg
    32881 ctacgctcgc aaaagcttga catgtacgct tatcgccata atctaaccct gtacgtatat
    32941 gtaataaata ctgtaatccg atacttaaat acataattgc cacgcataag aagaatggga
    33001 agaatgtctt ttcaaagtcc ggatataggc tgttagatag gaagaccatg atgaacatat
    33061 taaacatcat aaacgagacg tctttgaatg taacttgacc aaatcgattt gtaaaaaatg
    33121 tttgatgaga ccacattaac cataagaaca aactcatgac gatgtatttg aaaaataaat
    33181 cagctgaaat ggaaccgttt tgtgttgtta aaatcacatg tgcaattttt tgaatggcat
    33241 agacgaaaat taaatcaaag aacaactcat ggaatcctgc acgcttttca gctaaatgtt
    33301 ttggtgttaa tgcattaacc ataaaatttt aactccttta agatgtgtaa ttaatttact
    33361 aagtatacta tttatttttt ctagtgaata ggggcagatt tggcgatgaa gtggaaggag
    33421 aggtgactgc aaggtaattg cggaattaac aatcatcagc gatttaatat ttgactggag
    33481 acgtcatggt aataaaaaat tgatgagaaa ttgatggtga aaccagctgt gaatagcgat
    33541 gcaatgatag atagaattta attagagtca ttacgcgaaa tgattaatga taatttgtgg
    33601 taaatcaaag cataattttg tactatagat gaggatgata gagcatattt aagagtgtga
    33661 aatgttaaag tgaaaccgtt tacgtttccg attgcccaaa caaattacat cattgtataa
    33721 tatgatttgt taaatgcata acaagaatga aaatgtaaca tacgtagcaa ttggtttcat
    33781 aaattggatg ttagtggcgt attggttcat tagacgtatt agtaataaaa ttgtatatat
    33841 cataaggaga tgaatatgac atgacgagag tcgtattagc agcagcatac aggacaccta
    33901 ttggcgtttt tggaggtgcg tttaaagacg tgccagccta tgatttaggt gcgactttaa
    33961 tagaacatat tattaaagag acgggtttga atccaagtga gattgatgaa gttatcatcg
    34021 gtaacgtact acaagcagga caaggacaaa atccagcacg aattgctgct atgaaaggtg
    34081 gcttgccaga aacagtacct gcatttacag tgaataaagt atgtggttct gggttaaagt
    34141 cgattcaatt agcatatcaa tctattgtga ctggtgaaaa tgacatcgtg ctagctggcg
    34201 gtatggagaa tatgtctcag tcaccaatgc ttgtcaacaa cagtcgcttc ggttttaaaa
    34261 tgggacatca atcaatggtt gatagcatgg tatatgatgg tttaacagat gtatttaatc
    34321 aatatcatat gggtattact gctgaaaatt tagtagagca atatggtatt tcaagagaag
    34381 aacaagatac atttgctgta aactcacaac ataaagcagt acgtgcacag caaaatggtg
    34441 aatttgatag tgaaatagtt ccagtatcga ttcctcaacg taaaggtgaa ccaatcttag
    34501 tcactaagga tgaaggtgta cgtgaaaatg tatcagtcga aaaattaagt cgcttaagac
    34561 cagctttcaa aaaagacggg acagttacag caggtaatgc atcaggaatc aatgatggtg
    34621 ctgcgatgat gttagtcatg tcagaagaca aagctaaaga attaaatatc gaaccattgg
    34681 cagtgcttga tggctttgga agtcatggcg tagatccttc tattatgggt attgcaccag
    34741 ttggcgctgt agaaaaggct ttgaaacgta gtaaaaaaga attaagcgat attgatgtat
    34801 ttgaattaaa tgaagcattt gcagcacaat tattagctgt tgatcgtgaa ttaaaattac
    34861 ctcctgaaaa ggtgaatgtt aaaggtggcg ctattgcatt aggacaccct attggtgcat
    34921 ctggtgctag agttttagtg acattattgc atcaactgaa tgatgaagtt gaaactggtt
    34981 taacatcatt gtgtattggt ggcggtcaag ctatcgctgc agttgtatca aagtataaat
    35041 aataagaaaa caggttatca caacagtatt aattacatgt tggcataacc tgtttttatt
    35101 tgtttatgga tttattgggt aatattagtc atttgatggt ttaattgcaa atgctctaac
    35161 agggaaccca ggtgcatctt ttggtttagg gctgatagcg taaatgatgg cgccacgagt
    35221 tggtaattga tctaaattag ttaataactc gacttggtat ttatcctgac caagaatata
    35281 acgttcgcca actaaatcac cattttttac aacgtccaca gatgcatcgg tatcgaatgt
    35341 ttcatgacca acagcttcaa cacggcgttc ttcaattaag tacttcaaag catctaatcc
    35401 ccaacccggt gcatgttgtt gtccgttcgc atctttgttt tcaaactttt caatattagg
    35461 ccaacgtttt gaccaatcgg tacgaagtgc aacaaaagtg ccaggttcaa tagtaccatg
    35521 ctctttttcc catgcttcta tatgcgcacg tgttacgatg aaatcattgt tgttcgctac
    35581 ttctgttgaa aagtctaata caattaacgg caataccaat tcttttaaat caatgtcttc
    35641 taaataacgt ttgttctcga caaagtggat tggtgcatca atgtgagtac catattgcgt
    35701 tacaatattc caacgttgca catagaaacc atgatcttta accgtgaata aagttgaaac
    35761 ttcacctttt tcaaactcac taaaacgtgg tatttctgga tcaaatgtat gcgttaaatc
    35821 aacccaagtt gcttgtttta aagtatttaa ttgttgccat aaaggatatt gtgtcataaa
    35881 atcacccgtt tttagtttat tatatgataa atgctgcgat tattcttggc gtttagcttt
    35941 aacagcattc acaagtacag tcaatgcatc tttaacttct tcttcttttc gcgttttcaa
    36001 accacagtca gggtttaccc agaataatga gcggtcgatt tgttgtagtg aacgattgat
    36061 tgctgtagta atttcttctt ttgttggaat acgtggacta tgaatatcat atacacctag
    36121 accgatacct aaatcataat taatatcttc aaagtcttta attaaatcac catggctacg
    36181 agatgtttca attgaaataa catcagcatc taagtcatga atagcatgaa tgatttgacc
    36241 gaattgagaa taacacatat gtgtatggat ttgagtttca tcacgaactg aagacgttgc
    36301 aagtttaaat gataaaacag catctttaag atattgttcg tgatattcag agcgtaatgg
    36361 taagccttca cgtaatgcag gttcgtcaac ttggataact ttgattcctg cagcttcaag
    36421 tgctaatact tcttcgttga ttgctaaagc aatttgatct tgaacgactt tacgtggtaa
    36481 atcaacacgt tcaaatgacc agtttagaat tgttacaggt ccagttaaca tacctttaac
    36541 tggtttatct gttaagcttt gtgcataaac tgtttcatca acagttaaag gcgctgtcca
    36601 ttttacatca ccataaatga ttggtggttt tacggcgcgt gaaccatatg attgcaccca
    36661 accgaattta gttactaaga aaccttgtaa tttttctccg aagaattcaa ccatgtcatt
    36721 acgttcaaat tcaccgtgaa ctaatacatc taagccgatg tcttcttgaa ttttaatcca
    36781 tcgagcaatt tcatttttta agaatgtttc atatgcttcg tctgtaatgc gtttgttctt
    36841 ccaatctgca cggtattttc gaacttctcg gctttgtggg aatgatccaa tagttgttgt
    36901 tggtaaatcc ggtaagttca aacgtttttg ttgttgttca atacgttgcg cgaatggtga
    36961 ttgtcttgaa gtacgcacgc tttcgaaatc ataatctaag tttttgaatg attgattttg
    37021 gaaacgctca taacgtgctt ttaatttatc atatttaaca ctatcgtttt gattcaatag
    37081 gcgacgcaat gcatctaatt cgtctaattt ttcagttgca aagcttaagc cttcgccaac
    37141 acttgtatct aatgtttcat catctaaaga tactggaaca tgtaataatg aagatgatgg
    37201 ttgaatgaca agctcattag tgtgtgctaa caatttgtcg attaagactt ttttagcttc
    37261 aatatcactt gcccatacat tacgaccatc aataattcca gcgtataatg tttttgattt
    37321 atcaaaatct cctgcttcaa tttgtttaag gttatagcca ttatcatgga caaagtctaa
    37381 acctaaacca ccaacaggta aagaacttaa gaatttaaga tgtgcacgtt caaagtatgt
    37441 ttgaatgact aattttttag caacaccagc tttttcaaaa tagtcataag cttcacgtgt
    37501 aatattttca tagctttcgc tgtcgtctgt aactaagatt ggctcatcaa cttgaatgta
    37561 ctcagcacct gcatcaatta atgattcaaa cacttcttta taaagtggta ataacgtttt
    37621 aactttttct tcaaaagttt ggtgaccgcc ttttgataat ttaacaaaag taatcggacc
    37681 aacaatgaca gggtgagcgt taacgtttaa agattgggca tatttaaagc gatctaataa
    37741 tacattgcga ctcactttag gctcaacatt gtcccattca ggtacgatgt aatgatagtt
    37801 agtgttaaac cattttataa gtgcacttgc aacatggtct ttattaccgc gagcaatatc
    37861 aaataataaa tcatcatcaa tagttcttcc ttggaaacgt tcagggatga tgttgaataa
    37921 taatgacgta tctaatatat ggtcatataa agagaaatca ccaactggga tgctatctaa
    37981 gtgatagtac ttttgtaata ataaattttc tttatgtaga tcagttaatg tttgatctaa
    38041 ttcttcttta gaaatcttct ttgcccaata actttcgatg gcttttttcc attctctttt
    38101 tctacctaat cttgggaatc ctaagtttga tgttttaatt gttgtcataa tattgcctcc
    38161 ttgtgagcag taatagattt tgagtatgct gcaagttcta atgaatcttc gacattttga
    38221 aacggtgtga taatgtataa accattaaaa tattcatgaa cagtatcgat taaatccttt
    38281 gaaagcttaa gacttagttc tcgtgttttg gctttatcat ctttaactgc ttcaaattgt
    38341 tgtaaaattt catctgacat cttgattcct ggcacttcat tatgcaaaaa gagtgcgttt
    38401 ttgtaacttg cgataggcat aatgcctatg aaaaatggtt tgttcaagtg cttagtggca
    38461 tggtaaattt caatgatttt ctctttgctg tacacgggtt gtgttataaa ataagacatt
    38521 ccgctttcta tctttttctc taatcttttg acggcaccat ctaatttacg aacattaggg
    38581 ttaaaggcgc cagcgatgtt gaagtgtgta cgtttcttca gcgcatcacc gtcagtgtta
    38641 ataccttgat taaatcttag agcgagttca gttaatcctt tagaattaac atcatagaca
    38701 ttggttgcac ctggtaagtg accaactttt gaaggatcac cagttatggc taatatttcg
    38761 ttaacgccaa tgagcgataa tccaagtaaa tgggactgca agccgattaa gtttcggtct
    38821 cgacatgtaa tatgtacgag tggttcaata ttgtaatatt gcttaattaa gctagcagca
    38881 gcaatattgc taattctgac agttgccaat gaattatctg cgagtgttac cgcatctaca
    38941 ttagctttat caagtttagc gatattttca aaaaatctat ccgtgtctaa atgtttcggt
    39001 gtatccaatt cgataataac ggttggacgt tcttgaacct tagatgttaa tgattgtcta
    39061 actttatttt gagatggatt gaaaagtgct ttcgttggta tcggaatcac ttttttgtca
    39121 ttaacaggtt taagtgtctg aatagattct ttaataaatt tgatgtgctc tggcgttgta
    39181 ccacagcaac caccaattaa acgaacacct tcgcgaatta gattttgagc aacttgaccg
    39241 aaatattgtg cattgtcact atacttaaat tcactatttt caatatctaa taagctggca
    39301 tttggataac aagataagaa tgcgtgctct ggtaattcaa tatgtgtgaa agactcttgc
    39361 atatggtgcg gaccatgatg acaattgagt cccacgatgt ttgcaccaca ttgaacgagt
    39421 tgttttaatc cttcattgat tgcctgacca ttaactaagt aatttgtgtt tgaagcggtt
    39481 aattgagcaa tgattggaat gtcgtatttc tttctcgttc gtgaaatgac atttgttaac
    39541 tcttctaggt cgtaatacgt ttcgaaaagt agcgcgtcaa cgccttcttc aattaaggtg
    39601 tctatttgaa tttcagtatg ataaagaata gtttgtaagc tgatatcctc ttgtttgata
    39661 cctctaaacc caccaactgt gcctaatata tacgtatctt tatttgctgc tttttttgcg
    39721 atgcgaacgg cggcttgatg tattgcttta actttatctt caagaccgaa tcgttttaac
    39781 ttttcaaaat ttgcaccata agtattggtt tgaatgacat cagcaccggc ttcaatatat
    39841 gaacgatgga tgcgttcaac tttatctgga tggctaagat tatatgcttc tggacaggtg
    39901 tctaatcctt cagagtataa aatggttcca atagcgccat cagctactaa aacattatct
    39961 ttcaattgtg tgaggaattg actcattgaa tgcctccttt aatgcgtatt tgatgtctgc
    40021 aatgagttca tcaggatctt cgagaccaac acttaatcgg aatagaccga aagtgatacc
    40081 acgttcttgt ctcacttctt caggtagtgc agcgtgagac attgttgctg gatgtgaaag
    40141 gatcgtttca acaccaccca gactcactga aacgagtggt aatgtcagtg catcgacaaa
    40201 ttgttgtgct ttagactcat cagctaaacg aaagccaata acggcaccgc catttttagc
    40261 ttgttctaaa tgagcagtag tgagtcccgg ataataaact tctgaaattt catcttgctt
    40321 tattaaaaat gacacgattt tttgcgcgtt ttcgacagat tgtttaaatc tgattggaaa
    40381 tgtttttaaa tgtttagcaa gtgtccagct atcttgagca gataacatat tgcctgtacc
    40441 attttgtatt aaataaagag cttcactaat tgcctcatta ttagtaatga cagcaccagc
    40501 aattaaatcg ctatgtccac ttaaaaattt tgtagcacta tgaatgacaa tatcagcgcc
    40561 aagtaataaa ggtgattggc ctaacggtgt cataaatgta ttgtccacag ctaccagtag
    40621 ttcatgcttt tcggctattt tagaaacagc tttgatatca gtaattttaa aacagggatt
    40681 cgatggtgtt tcgatataaa ttaattttgt gtttgattga atggcaccct cgatttgttc
    40741 gagcttggta gtatctacgg ttgtaaattc aatattaaat cgattcaaaa tttgctcagt
    40801 gaggcgaaaa gtaccgccat atacatcatc gggtaagatg acatgatcac cagatttgaa
    40861 agtcaaaagt actgctgaaa tagcagcaat acctgatgca aaagcaaaag cgaattttcc
    40921 ctgttctaat cgtgctaact tctcttctaa aagttcacgg ttagggttgc cacttcgtgc
    40981 ataatcatat ttaacatcgc caccaagact tgtttgatgg aatgttgaag aatcatagag
    41041 tggtgggtta gctgaatgat attccacacc tctacgccaa tcgaatatca cttctgtctc
    41101 ttttgaaagt gtcatacaat ctctccaatc tgagctttat ctaatgcttg gatgatatcg
    41161 cgttcgatgt cttcataatt ttcaacacct agtgataagc ggattaaata ctcatcaatg
    41221 ccacgtttat ctttttcagc atctggcata tcaacatgtg tttgggtgta agggaaggtc
    41281 actaatgttt cagtacctcc taaactttct gcaaaaatac aaatgtctaa attttctaat
    41341 aatttagcga cgctataggc cttgttaagt cttaaactaa gcatgccagt ttgcccgcta
    41401 tatagtactt cgtcaattgc ttgaagtgac tgacattttt tagcaagttt tctagcgttt
    41461 gattgcgcac gctcaatgcg taaatgcaaa gttttaagtc cacgtaacaa caaataacta
    41521 tctattggtg aaagtgttgc gccagtcatg ttgtgaaaat caaacaactg ttgcgcgagt
    41581 gattcatctt tgacggttac gacacctgct agtacatcgt tatgtccacc aatatatttc
    41641 gtagctgaat gtaagactat atcagcacct tctgctaatg gtgttgaaag ataaggtgtt
    41701 aaaaaagtat tgtcgataat tgacaataag cctttagctt tacaaagttg atagtacggc
    41761 tttacatcaa tagcaatcat ttgtgggtta gatattggtt caatgaataa tgcaactgtt
    41821 ttatcagtga tttctttttc aacttgttca taatctgtaa aatcaacgta cttaaatttg
    41881 atatcgtatt gttgctcgta aaattcaaat aatctaaatg tgccaccata taaatcgaat
    41941 gaaactaaaa tttcatcatg aggtttaaat agattacata ttaattgaat ggctgacatt
    42001 ccacttgatg tagcgaatga tgcaatacca tgctcaagtt tggcaaaaca ggtttcaaat
    42061 gttgagcgtg taggattttt agtacgtgta taatcaaatc ctgtcgattg tcctagtttt
    42121 ggatgtttgt aggcagtaga taaatggatt ggattcgcta tagcaccggt tgaatcatca
    42181 gttaatgtga tttgggctaa ctgtgtatcc ttcatattaa gaccctccta taagaaaaaa
    42241 taaaaagctt ccgtccttcg tacccgaatg aatcggataa aaaggacgaa agcttatgtt
    42301 tcgcggtacc acctttattt gttattccat cgctgaaata accttattca gtacgcatta
    42361 aaagtaaata tgcttactga acaattatca caattaaagt cagtaagtaa ggatatagta
    42421 atgtgctatc ccatacttat taacaaaaaa tcgtgcgtaa agaatccagt acgccattta
    42481 acatcaatgt taatactgta tcgctataac gggcgaaccc gtagacacct catattggca
    42541 tcaacactcc aaggccattt tcaaacacgc tttcaaaatc ttctctcagc tactaaagac
    42601 tctctgtata agcagggtgt gttttacttt cctctttatt gtgtttacgt ttcattaaac
    42661 tattataaga tattaattag cttacagagt aaaaaaagat ttgtcaacaa ttattcagaa
    42721 aattttgatt taaaagttaa tttgtttgtg aaattgtaat tggtatcttg aagttgaaaa
    42781 atgaattatt ttttaaataa agtgtggtta atggttgtct gactcattta gaatacataa
    42841 aatatattta actgttgtta tcaaataaaa agtgatgtga gtgaattgtc aaaaagtgaa
    42901 gatcaacgta ttactaaaac aaaagatgaa caaattaagc aaatagatat atcggatatc
    42961 aaaccgaatc cgtatcagcc ccgaaaaact ttcgatgaaa atcatttaaa tgatttggca
    43021 gattcaatta agcaatatgg aattttgcaa ccaattgtgc ttagaaaaac agttcaaggt
    43081 tattacattg tagttggtga aagaaggttt agagcttcga aaattgctgg tctaaaatac
    43141 gtatcagcga ttatcaaaga tttaacagat gaagatatga tggaactggc ggtcatcgaa
    43201 aatttacaac gagaagactt aaatgcgatt gaagaagctg aaagttatca acgtttgatg
    43261 acagatttga aaattacaca acaagaagta gcgaagcgat tgagtaagtc gcgcccgtat
    43321 atagcgaata tgttgaggtt attacatttg ccgaaaaaga ttgctgacat ggtaaaagat
    43381 gggcgactga caagtgcaca tggacgaacg ttattggcaa ttaaagatga acaacaaatg
    43441 cttaggttag cgaaacgggt tgttaaagaa aagtggagtg ttagatattt agaaaaccat
    43501 gttaatgaat taaaaaatgt ttcgtcaaag tcggaaacag acaaagtaga tataactaag
    43561 cctaaattta taaagcaaca agaacgacag ttgcgagaac agtatggtac caaagtagat
    43621 atatcaataa aaaaatcggt tggtaaaatc tcatttgagt ttgattcaca agatgacttt
    43681 gtgagaataa ttgaacaatt aaatcgtagg tatggtaaat agttacacaa ttttatataa
    43741 taactctttg tgaatctata gatatattga tagttatcta tattctttga agatgatgcg
    43801 acttcagtag gttggaagaa tacaacaata ttaataaaat tatttgagac tatatagaag
    43861 ggagtaacgc taattatgga tcaagttatg agtattattt catcgctatt cgaaccatta
    43921 actaaaattg aaacatatga aaatattgca actaaaatcg ctatgattgt tatttatatt
    43981 atcgtagccc tcatagttat taaaatactg aataaaatga ttgaacaggg atttaagatt
    44041 caaaataaaa gtaaaaagag taacaaaaag cgctctaaaa ctttaatatc tcttgttcaa
    44101 aatgtagtga agtatatcgt ttggtttata gttattacga cgattttaag taaatttggc
    44161 attagtgttg aaggtgttat tgcaagtgct ggtgtcgtag gtttagcagt aggttttggt
    44221 gcgcagacca ttgttaaaga cgttattact ggatttttca ttatatttga aagccaattt
    44281 gatgtaggtg attatgttaa gataaacaac ggtggtacaa ctgtagcaga gggaacagtt
    44341 aaatcaattg gactccgttc aacacgtatt aatacaatat ctggagaact tacaatcttg
    44401 ccaaatagta gcatgggtga aataacgaat tactcaatta cgaatggaac agcaattgtt
    44461 aaaattccag tatctgtaga tgaaaatatt gataatgttg agaagaaatt aaacaaatta
    44521 tttacatctc tgcgtagtaa atattattta tttgtaagtg atcctgttgt gattggtatt
    44581 gatgcctttg aagatactag agtaatatta agggtttcag cagaaacaat accaggtgaa
    44641 ggtttctctg gtgcgcgtat tattcgtaaa gaagtacaaa aaatgttttt acaagaaggt
    44701 atcaagacac cacaaccaat aatgacacct tttaatcata gtgaaaacgg tgtttagtag
    44761 tttataatac atggaggtca tatttaatgg cgtcaaaata tggaataaat gatatagtag
    44821 aaatgaaaaa acaacatgcg tgtggaacca atcgttttaa aattattaga atgggtgctg
    44881 acattagaat taagtgtgaa aattgtcaac gaagcattat gattccacgt caaacgtttg
    44941 ataaaaaact aaaaaaaatc atcgtatctc aggatgatac acaaagatag gagaatgatt
    45001 catggcttta acagcaggta tcgttggctt accaaacgtt ggtaaatcaa cattatttaa
    45061 tgcaattaca aaggcgggtg ccttggcagc aaactatccg ttcgcaacta tagacccaaa
    45121 tgtgggtatt gtagaagtgc cagatgcaag actacttaaa ttagaagaaa tggttcaacc
    45181 taaaaaaaca ttacctacta catttgaatt tacagatatt gccggaattg ttaaaggtgc
    45241 ttctaaaggt gaaggtttag gtaataaatt cttatcacat attagagaag tagatgcaat
    45301 ttgtcaggtt gttcgtgctt ttgacgatga taatgtaaca catgtagctg gtcgagttga
    45361 tccaattgat gacatcgaag ttatcaatat ggaattagta cttgcagact tagaatcagt
    45421 tgaaaaacgc ttacctagaa ttgaaaaatt ggcacgtcaa aaagataaga ctgctgaaat
    45481 ggaagtgcgt attttaacaa ctattaaaga agctttagaa aatggtaaac ccgctcgtag
    45541 tattgacttt aatgaagaag accaaaaatg ggtgaatcaa gcgcaattac tgacttctaa
    45601 aaaaatgctt tatatcgcta atgttggtga agatgaaatt ggtgatgatg ataatgataa
    45661 agtaaaagcg attcgtgaat atgcagcgca agaagactct gaagtgattg ttattagtgc
    45721 aaaaattgaa gaagaaattg ctacattaga tgatgaagat aaagaaatgt tcttagaaga
    45781 tttaggtatc gaagaaccag gattagatcg attaattaga acaacttatg aattattagg
    45841 attatcaaca tattttactg ctggtgtgca agaagtacgt gcttggacat ttaaacaagg
    45901 tatgactgca cctcaatgtg ctggtatcat tcatactgat tttgaacgtg gatttatccg
    45961 tgccgaagta acaagttatg atgactatgt acaatatggc ggcgaaagtg gcgctaaaga
    46021 agcgggcaga caacgattag aaggtaaaga atatattatg caagatggcg atatcgttca
    46081 tttcagattt aatgtataaa cgatagagtg aagttaatta aatagtatat atgtagaaga
    46141 ggcggaatca attgttcgcc tcttttaatt atgcgtataa tttattaaaa gaatggaatg
    46201 attttactcg cgtaaataat atcttgagtg ctgaaaaatt gtttgccttc gccagtataa
    46261 gcaggctcta aaacaagatt agcctttgca caataaagcc attcaggatg aatgccacta
    46321 ttaagtatct cttggaattc ttgaaaatct ttagaccaat caatatttaa attcattcct
    46381 taccacctca caaccattat agaacatgcg ttcgcttttg tgaagtgtat ttttaaaaat
    46441 atcatagaaa atttcaaatg aattaatgtc aaaaatgata tgaagagtag tttgaattat
    46501 tattgtaaaa agtaatggcg catgatataa ttctttattg tgagtaatga aaattattcc
    46561 ttgcttatct gttttaagat tgataagccg tatagaccac aaggaggtgc aaatataaaa
    46621 tgagaacata tgaagttatg tacatcgtac gcccaaacat tgaggaagat gctaaaaaag
    46681 cgttagttga acgtttcaac ggtatcttag ctactgaagg tgcagaagtt ttagaagcaa
    46741 aagactgggg taaacgtcgc ctagcttatg aaatcaatga tttcaaagat ggcttctaca
    46801 acatcgtacg tgttaaatct gataacaaca aagctactga cgaattccaa cgtctagcta
    46861 aaatcagtga cgatatcatt cgttacatgg ttattcgtga agacgaagac aagtaataat
    46921 tagagggggc gtttaaatgc taaatagagt tgtattagta ggtcgtttaa cgaaagatcc
    46981 ggaatacaga accactccct caggtgtgag tgtagcgaca ttcactcttg cagtaaatcg
    47041 tacgttcacg aatgctcaag gggagcgcga agcagatttt attaactgtg ttgtttttag
    47101 aagacaagca gataatgtaa ataactattt atctaaaggt agtttagctg gtgtagatgg
    47161 tcgcttacaa tcccgtaatt atgaaaatca agaaggtcgt cgtgtgtttg ttactgaagt
    47221 tgtgtgtgat agcgttcaat tccttgaacc taaaaatgcg caacaaaatg gtggccaacg
    47281 tcaacaaaat gaattccaag attacggtca aggattcggt ggtcaacaat caggacaaaa
    47341 caattcgtac aataattcat caaacacgaa acaatctgat aatccatttg caaatgcaaa
    47401 cggaccgatt gatataagtg atgatgactt accattctaa taaaaattaa cgaaattaaa
    47461 gcgaaaaaat tatcaaagga ggcacacaat catggcaggt ggaccaagaa gaggcggacg
    47521 tcgtcgtaaa aaagtatgct atttcacagc aaatggtatt acacatatcg actacaaaga
    47581 cactgaatta ttaaaacgtt ttatctcaga acgcggtaaa attttaccac gtcgtgtaac
    47641 tggtacttca gctaaatatc aacgtatgtt gactacagct atcaaacgtt ctcgtcatat
    47701 ggcattatta ccatatgtta aagaagaaca ataatattta atatattata accctcgtag
    47761 gcataggctt atgggggatt tttttatgtt tgggatatag aaaagggaca gaaaagggca
    47821 aaaaaggatg atgtgaatgt tttgtgttcg gaatttgcac aaagatatgt ttatattgca
    47881 aaaataatat gaatttagat gcataaaaaa agaactacgc attttaaata aaatgcatag
    47941 ctcttctttt tcttgcatac gaattaaaat aactcgcgag acctataagt ctctttcctc
    48001 actagatagt ttatactttt ggtctgttga agtcaataat tttatctaaa gctataaaaa
    48061 atcttttgat agctaatgca ttattataat agctttcgtt tcttttatat cgcttttgaa
    48121 gttggtccaa atcgtgatat cttgcttgga taattgcatt actacaaact tgattatgta
    48181 attctagcgt agcgaaagta tctatgaaat tttttattcc gaacatgttt ctagatatgc
    48241 ctatattatt ccctttttca aataaatatt gaggagatct actgtcataa tttagatttg
    48301 ctatgatggg ttggttatga gctgatttgt ttcttatatt tttaactaaa ggcattaaaa
    48361 tattagcaac tctcaattct tcgtcattgt acttcttgta atagaagttg agaaacgaaa
    48421 cgaattgacc tagttgcatg aattcaatgc aaacccatgc gggtggattt tgatagtatt
    48481 tattcaactt ctcgggtagt tgtcctcgtt tattcatatg cttgaatatt tcgtttttat
    48541 ttttgatttt ggtttccata acttcttctg gtgttcttga atttgtgtca aaatttgaat
    48601 tgctatatga tttatcaata cataagaact catctattat tttataacca tcttcttggt
    48661 tattttctgt tattagtttt aagactagac actttaaact atgttcaata tctaaagtta
    48721 aatgcaacat tgtgtatctt aatttcatat ctatagttgc taaatctgat aaataagcaa
    48781 attctatgaa atagccgcca ttcttttttt cgaaattttt tcggaaataa gctagtttga
    48841 agaagtaatt attttttcta agaatttcat ttgctttttc ggtgtcaata atattaaaaa
    48901 atatattcat ctgttttaat ttcgctattt gctcatcaaa attgagcata ggcttaattt
    48961 ctgctagtat atcatctttt tccaatttta actccccaat cgttcaaatt tattcatcat
    49021 atctttcgcc atctgattag taacatgtgt gtatatctct agatttttta taatctgaat
    49081 gacctacatg ctcttgcatt gcttttaagt taattcctaa ttgagcaagt gtagatatat
    49141 gcgaatgatg taatgtatgc gtcgttacag gtttcttaat agaactaatc tcagttgtct
    49201 ctttgatagt gcccccttta ataatgttgc taattttgtt cgagtcgata gggccaccag
    49261 ctgcatttgt gaatatgtac cctatattaa taaacttatc attacaagca taagtgttct
    49321 tagtaagtcg atgctatgga tagtgaggcc tatagcccta tagctgttac ttgttctagt
    49381 tgtctctttt actccggatg ctcccgtcat ttttcagtta tccaattaac tttaccgtcg
    49441 atatctagcg ttttatcttc atagtttata tttactctct ttattgcaag tagctcaccg
    49501 atacgcatgt cattagcaat ttgaaactgt accatagctt ttaccatttt ataattacgt
    49561 tttgtcgttg gatattttta tacttaatta catagtcgaa acaatccagt aactccttaa
    49621 cttcattatc ttctaatgtg ttactatgtt tagctagtaa cgcatcact
//
LOCUS       DAHHSC010000002        45421 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.002, whole genome shotgun sequence.
ACCESSION   DAHHSC010000002 DAHHSC010000000
VERSION     DAHHSC010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 45421)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45421)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45421
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.002"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            complement(<1..350)
                     /locus_tag="NBI57_000051"
     CDS             complement(<1..350)
                     /locus_tag="NBI57_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1672 family protein"
                     /protein_id="HCD1821050.1"
                     /translation="MFKRIKLILIATIILSGCSTTNNESNNETKSVPEEMEASKYVGQ
                     GFQPPAEKDAIEFAKKHRKEFEKVGEQFFKDNFGLKVKATNVVGKDDGVEVYVHCEDH
                     GIVFNASLPLYKDA"
     gene            complement(408..1313)
                     /locus_tag="NBI57_000052"
     CDS             complement(408..1313)
                     /locus_tag="NBI57_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020807974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1672 domain-containing protein"
                     /protein_id="HCD1821051.1"
                     /translation="MFKRTKLILIATILLSGCSTTNNESNNETKSVPEEMEASKYVGQ
                     GFQPPAEKDAIEFSKKHKDKIAKRGEQFFMDNFGLKVKATNVVGSGDGVEVFVHCDDH
                     DIVFNASIPFDKSIIESDSSLRSEDKGDDMSTLVGTVLSGFEYRAQKEKYDNLYKFLK
                     ENEKKYQYTGFTKEAINKTQNSGYENEYFYIVANIPTLQEYRKYYEPLIKKNNLNFKK
                     GMKQARKGVGYKAAIEVHTTLFSRSSNFSKDKKLDDVLDLSESTKKLHLNFENTKIFL
                     QLAKSTISTNRVNYSDNESIRIEVE"
     gene            complement(1403..1618)
                     /locus_tag="NBI57_000053"
     CDS             complement(1403..1618)
                     /locus_tag="NBI57_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000126131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1821052.1"
                     /translation="MTALPSHYDDMFILNTQAHARLDQMAQQFEVVCNGLNENEGQAI
                     QTMDQSASLIRSNLIQVKEQLEKLAVY"
     gene            complement(2340..3794)
                     /locus_tag="NBI57_000054"
     CDS             complement(2340..3794)
                     /locus_tag="NBI57_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HCD1821053.1"
                     /translation="MLITKNQAEKWFDNSLGKQFNPDLFYGFQCYDYANMFFMIATGE
                     RLQGLYAYNIPFDNKARIEKYGQIIKNYDSFLPQKLDIVVFPSKYGGGAGHVEIVESA
                     NLNTFTSYGQNWNGKGWTNGVAQPGWGPETVTRHVHYYDDPMYFIRLNFPDKVSVGDK
                     AKSVIKQATAKKQAVIKPKKIMLVAGHGYNDPGAVGNGTNERDFIRKYITPNIAKYLR
                     HAGHEVALYGGSSQSQDMYQDTAYGVNVGNKKDYGLYWVKSQGYDIVLEIHLDAAGES
                     ASGGHVIISSQFNADTIDKSIQDVIKNNLGQIRGVTPRNDLLNVNVSAEINMNYRLSE
                     LGFITNKKDMDWIKKNYDLYSKLIAGAIHGKPIGGLVAGNVKTSAKNQKNPPVPAGYT
                     PDKNNVPYKKETGYYTVANVKGNNVRDGYSTNSRITGVLPNNTTITYDGAYCINGYRW
                     ITYIANSGQRRYIATGEVDKAGNRISSFGKFSTI"
     gene            complement(3805..4107)
                     /locus_tag="NBI57_000055"
     CDS             complement(3805..4107)
                     /locus_tag="NBI57_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HCD1821054.1"
                     /translation="MDAKVITRYIVLILALVNQFLANKGISPIPVDEESVSSIILTVV
                     ALYTTYKDNPTSQEGKWANQKLKKYKAESKYRKATGQAPIKEVMTPTNMNDTNDLG"
     gene            complement(4243..4542)
                     /locus_tag="NBI57_000056"
     CDS             complement(4243..4542)
                     /locus_tag="NBI57_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2951 domain-containing protein"
                     /protein_id="HCD1821055.1"
                     /translation="MFGFTKRHEQDWRLTRLEENDKTMFEKFDRIEDSLRTQEKIYDK
                     LDRNFEELRRDKEEDEKNKEKNAKNIRDIKMWILGLIGTILSTFVIALLKTIFGI"
     gene            complement(4588..4752)
                     /locus_tag="NBI57_000057"
     CDS             complement(4588..4752)
                     /locus_tag="NBI57_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HCD1821056.1"
                     /translation="MLKLISPTFEDIKTWYQLKEYSKEDIAWYVDMEVIDKEEYAIIT
                     GEKYPENLES"
     gene            complement(4745..5134)
                     /locus_tag="NBI57_000058"
     CDS             complement(4745..5134)
                     /locus_tag="NBI57_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2977 domain-containing protein"
                     /protein_id="HCD1821057.1"
                     /translation="MQILVNKRNEIISYAIIGGFEEGIDIENLPENFSQVFRPKAFKY
                     SNGEIVFNEDYSEEKDDLHQQIDSEEQNTVASDDILRKMVASMQKQVVQSTKLSMQVN
                     KQNALMAKQLVTLNKKLEEVKGETENA"
     gene            complement(5134..6600)
                     /locus_tag="NBI57_000059"
     CDS             complement(5134..6600)
                     /locus_tag="NBI57_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate upper protein"
                     /protein_id="HCD1821058.1"
                     /translation="MSNLEKSVAINLENTAHYENISNLDITFRTGESDSSVLLFNIIK
                     NNQPLLLSEENIKARIAIRGKGVMVVAPLEILDPFKGILKFQLPNDVIKRDGSYQAQV
                     SVAELGNSDVVVVERTITFNVEKSLFSKIPSETKLHYIVEFQELEKTIMDRAKAMDEA
                     IKNGEDYASLIEKAKEKGLSDIQIAKSSSIDELKQLANSRISDLENKAQAYSRTFDEQ
                     KRYMDEKHEAFKQSVNSGGLVTSGSTSNWQKAKITKDDGKIMQITGFDFNNPEQRIGD
                     STQFIYVSQAINYPRDVSTNGTVEYLVVTSDYKRMTYRPNGTNKVFVKRKEAGSWSEW
                     SELAINDYNTPFETVQSAQSKANMAESNAKLYADDKFNKRYSVIFDGTANGVGSTLYL
                     NESLDQFILLIFYGTFPGGDFTEFGSPFGGGKISLNPSNLPDGDGNGGGVYEFGLTKS
                     SRTSLTISNDVYFDLGSQRGSGANANRGTINKIIGVRK"
     gene            complement(6600..8510)
                     /locus_tag="NBI57_000060"
     CDS             complement(6600..8510)
                     /locus_tag="NBI57_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1821059.1"
                     /translation="MENLYLIKDLGALAGRDYRAKEIQNLQRIEQFALGLTTEFKLHQ
                     KAKTMQHFAEQIYYNGRSQAAVNKSLQSQINALVVAPRNNSANEIVQARVNVNGETFD
                     TLKEHLDDWETKTQINKEETIRELNKTKQEILDIEYRFEPDKQEFLFVTELAPLTNAV
                     MQSFWFDNRTGIVYMTQARNNGYMLSRLRPNGQFIDSSLIVGGGHGTHNGYRYIDDEL
                     WIYSFILNGNNENTLVRFKYTPNVEISYGKYGMQDVFTGHPEKPYITPVINEKENKIL
                     YRIERPRSQWELENSMNYIEIRSLDDVDKNIDKVLHKISIPMRLTNETQPMQGVTFDE
                     KYLYWYTGDSNPNNRNYLTAFDLETGEEAYQVNADYGGTLDSFPGEFAEAEGLQIYYD
                     KDSGKKALMLGVTVGGDGNRTHRIFMIGQRGILEILHSRGVPFIMSDTGGRVKPLPMR
                     PDKLKNLGMLTEPGLYYLYTDHTVQIDDFPLPREWRDAGWFLEVKPPQTGGDVIQILT
                     RNSYARNMMTFERVLSGRTGDISDWNYVPKNSGKWERVPSFITKMSDINIVGMSFYLT
                     TDDTKRFTDFPTERKGVAGWNLYVEASNTGGFVHRLVRNSVTASAEILLKNYDSKTSS
                     GPWTLHEGRIIS"
     gene            complement(8526..8816)
                     /locus_tag="NBI57_000061"
     CDS             complement(8526..8816)
                     /locus_tag="NBI57_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1821060.1"
                     /translation="MATEEVKIKALLENDKQYFPATHWKAINGIPYAGSSDIDGLPQD
                     GIISVDDKNKLDKLKIGEAGIIQNSIVQKSPNGKLWKITVDDSGKLGTVLFY"
     gene            complement(8816..10399)
                     /locus_tag="NBI57_000062"
     CDS             complement(8816..10399)
                     /locus_tag="NBI57_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCD1821061.1"
                     /translation="MDYHDHLSVMDFNELICENLLDVDYGSFKEYYELNEARYITFTV
                     YRTTHNSFVFDLLICENFIIYHGEKYTIKQTAPKVEGDKVFIEVTAYHIMYEFQNHSV
                     ESNKLDDDSSETGKTPEYSLDEYLRYGFANQKTSVKMTYKIIGDFKRKVPIDELGNKN
                     GLEYCKEAVDLFGCIIYPNDTEIGFYSPETFYQRSEKVIRYQYNTDTVSATVSTLELR
                     TAIKVFGKKYTAEEKKNYNPIRTTDIKYSNGFIKEGTYRTETIGSKATINFDCKYGNE
                     TVRFTIKKGSQGGIYKLILDGKQIKQISCFAKSVQSETIDLIKNIDKGKHVLEMIFLG
                     EDPKNRIDISSNKKAKPCMYVGTEKSTVLNLIADNSGRNQYKAIVDYVADSAKQFGIR
                     YANTQTNEDIETQDKLLEFAKKQINDTPKTELDVNYIGYEKIEPRDSVFFVHELMGYN
                     TELKVVKLDRSHPFVNAIDEVSFSNEIKDMVQIQQALNRRVIAQDNRYNYQANRINHL
                     YTSTLNSPFETMDIGSVLI"
     gene            complement(10408..11232)
                     /locus_tag="NBI57_000063"
     CDS             complement(10408..11232)
                     /locus_tag="NBI57_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail family protein"
                     /protein_id="HCD1821062.1"
                     /translation="MQSFVKIIDGYKEEVITDFNQLIFLDARAESPNTNDNSVTINGV
                     DGILPGAISFAPFSLVLRFGYDGIDVIDLNLFEHWFRSVFNRRHPYYVITSQMPGVKY
                     AVNTANVTSNLKDGSSTEIEVSLNVYKGYSESVNWTDSEFLFDSNWMFENGIPLDFTP
                     KYTHTSNQFTIWNGSTDTINPRFKHDLKILINLNASGGFELVNYTTGDIFKYNKSIDK
                     NTDFVLDGVYAYRDINRVGIDTNRGIITLAPGKNEFKIKGDVSDIKTTFKFPFIYR"
     gene            complement(11232..17408)
                     /locus_tag="NBI57_000064"
     CDS             complement(11232..17408)
                     /locus_tag="NBI57_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HCD1821063.1"
                     /translation="MNEKVEGMTLELKLDHLGVQEGMKGLKRQLGVVNSEMKANLSAF
                     DKSEKSMEKYQARIKGLNDRLKVQKKMYSQVEDELKQVNANYQKAKSSVKDVEKAYLK
                     LVEANKKEKLALDKSKEALKSSNTELKKAENQYKRTNQRKQDAYQKLKHLRDAEQKLK
                     NSNQATTAQLKRASDAVQKQSAKHKALVEQYKQEGNQVQKLKVQNDNLSKSNDKIESS
                     YAKTNTKLKQTEKEFNDLNNTIKNHSANVAKAETAVNKEKAALNNLERSIDKASSEMK
                     TFNKEQMIAQSHFGKLASQADVMSKKFSSIGDKMTSLGRTMTMGVSTPITLGLGAALK
                     TSADFEGQMSRVGAIAQASSKDLKSMSNQAVDLGAKTSKSANEVAKGMEELAALGFNA
                     KQTMEAMPGVISAAEASGAEMATTATVMASAINSFGLKASDANHVADLLARSANDSAA
                     DIQYMGDALKYAGTPAKALGVSIEDTSAAIEVLSNSGLEGSQAGTALRASFIRLANPS
                     KNTAKEMKKLGIHLSDAKGQFVGMGELIRQFQDNMKGMTREQKLATVATIVGTEAASG
                     FLALIEAGPDKINSYSKSLKNSNGESKKAADLMKDNLKGALEQLGGAFESLAIEVGKD
                     LTPMIRAGAEGLTKLVDGFTHLPGWVRKASVGLALFGAAIGPAVLAGGLLIRTVGSAA
                     KGYASLNRRIAENTILSNTNSKAMKSLGLQTLFLGSTTGKTSKGFKGLAGAMMFNLKP
                     INVLKNSAKLAILPFKLLKNGLGLAAKSLFAVSGGARFAGVALRFLTGPIGATITAIT
                     IAYKVFKTAYDRVEWFRNGINGLGETIKFFGGKIIGGAVRKLGEFKNYLGSIGKSFKE
                     KFSKDMKDGYKSLSDDDLLKVGVNKFKGFMQTMGTASKKASDTVKVLGKGVSKETEKA
                     LEKYVHYSEENSRIMEKVRLNSGQISEDKAKKLLKIETDLSNNLIAEIEKRNKKELEK
                     TQELIDKYSAFDEQEKQNILTRTKEKNDLRIKKEQELNQKIKELKEKALSDGQISENE
                     RKEIEKLENQRRDITVKELSKTEKEQERILVRMQRNRNAYSIDEASKAIKEAEKARKA
                     RKKEVDKQYEDDVIAIKNNVNLSKSEKDKLLAIADQRHKDEVRKAKSKKDAVVDVVKK
                     QNKDIDKEMDLSSGRVYKNTEKWWNGLKSWWSNFREDQKKKSDKYAKEQEETARRNRE
                     NIKKWFGNAWDGVKSKTGEAFSKMGRNANHFGGEMKKMWSGIKGIPSKLSSGWSSAKS
                     SVGYHTKAIANSTGKWFGKAWQSVKSTTGSIYNQTKQKYSDASDKAWAHSKSIWRGTS
                     KWFSNAYKSAKGWLTDMANKSRAKWDNISSTAWSNAKSVWKGTSKWFSNSYKSLKDWT
                     GDMYSRAHDRFDAISSSAWSNAKSVFNGFRKWLSKTYDWIRDIGKDMGRAAADLGKNV
                     ANKAIGGLNSMIGGINKISKAITDKNLIKPIPTLSTGTLAGKGVATDNSGALTQPTFA
                     VLNDRGSGNAPGGGVQEVIHRADGTFHAPQGRDVVVPLGVGDSVINANDTLKLQRMGV
                     LPKFHGGTKKKDWLDQLKGNIGKKAGEFGATAKNTAHNIKKGAEEMVEAAGDKIKDGA
                     SWLGDKIGDVWDYVQHPGKLVNKVMSGLNINFGGGANATVKIAKGAYSLLKKKLVDKV
                     KSWFEDFGGGGDGSYLFDHPIWQRFGSYTGGLNFNGGRHYGIDFQMPTGTNIYAVKGG
                     IADKVWTDYGGGNSIQIKTGANEWNWYMHLSKQLARQGQRIKAGQLIGKSGATGNFVR
                     GAHLHFQLMQGSHPGNDTAKDPEKWLKSLKGGGGKVGGSGYENAKRAILRAQSILGGR
                     YRSDYITTQMLRVAKRESNYQADAINNWDSNARAGTPSKGMFQMIEPSFRAFAKPGHG
                     NIYNPTDEAISAMKYIVAKYGWGGFKRAGDYAYANGGLITKHQIAEVGEGDKPEMVIP
                     LTRRKRAMQLTEQVMRIIGMDGKQNNITVNNDTSTVEKLLKQIVMLTDKGNKLTDALI
                     QTVSSQDNNLSSSDAIRDLEKVLSKQSGHRANANNYMGGLTN"
     gene            complement(17422..17580)
                     /locus_tag="NBI57_000065"
     CDS             complement(17422..17580)
                     /locus_tag="NBI57_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1821064.1"
                     /translation="MEQYGWTLTEVRKQPYVKLLEILNEENKEETEEKQSEQKVITGT
                     DLRKLFGS"
     gene            complement(17622..17972)
                     /locus_tag="NBI57_000066"
     CDS             complement(17622..17972)
                     /locus_tag="NBI57_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1821065.1"
                     /translation="MIKFEIKDRKTGKTESYTKEDVTMGEAEKCYEYLELVNQENKKE
                     APNATKMRQKERQLLVDLFKDEGLTEEDVLNKMSTKTYTKALQDIFREINGEDEEDSE
                     TEPEEMGKTEEQSQ"
     gene            complement(18030..18485)
                     /locus_tag="NBI57_000067"
     CDS             complement(18030..18485)
                     /locus_tag="NBI57_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig domain-containing protein"
                     /protein_id="HCD1821066.1"
                     /translation="MTKTLKVYKGDDVVASEQGEGKVSVTLSNLEADTTYPKGTYQVA
                     WEENGKESSKVDVPQFKTNPILVSGVSFTPETKSIMVNTDDNVEPNIAPSTATNKILK
                     YTSEHPEFVTVDENTGAIHGVAEGTSVITAMSTDGSDKSGQISVTVTNG"
     gene            complement(18577..19218)
                     /locus_tag="NBI57_000068"
     CDS             complement(18577..19218)
                     /locus_tag="NBI57_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCD1821067.1"
                     /translation="MAEGQGSYKVGFKRLYVGVFNPEATKVVKRMTWEDEKGGTVDLN
                     ITGLAPDLVDMFASNKRVWMKKQGTNEVKSDMSIFNIPSEDLNTVIGRSKDKNGTSWV
                     GENTRAPYVTVIGESEDGLTGQPVYVALLKGTFSLDSIEFKTRGEKAEAPEPTKLTGD
                     WMNRKVDVDGTSQGIVYGYHEGKEGEAEFFKKVFVGYTDSEDHSEDSAGSLPS"
     gene            complement(19253..19648)
                     /locus_tag="NBI57_000069"
     CDS             complement(19253..19648)
                     /locus_tag="NBI57_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3168 domain-containing protein"
                     /protein_id="HCD1821068.1"
                     /translation="MNILNTIKEILLSDAELQTYINSRIYYYKVTENAETSKPFVVIT
                     PIYDLPSDFMSDKYLSEEYLIQIDVESSNNQKTIDITKRIRYLLYQQNLIQASSQLDA
                     YFEETKRYVMSRRYQGIPKNIYYKNQRIE"
     gene            complement(19649..20050)
                     /locus_tag="NBI57_000070"
     CDS             complement(19649..20050)
                     /locus_tag="NBI57_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1821069.1"
                     /translation="MSVEIKGIPEVLKKLESVYGKQSMQAKSDRALNEASEFFIKALK
                     KEFESFKDTGASIEEMTKSKPYTKVGSQERAVLIEWVGPMNRKNIIHLNEHGYTRDGK
                     KYTPRGFGVIAKTLAANERKYREIIKKELAR"
     gene            complement(20047..20379)
                     /locus_tag="NBI57_000071"
     CDS             complement(20047..20379)
                     /locus_tag="NBI57_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1821070.1"
                     /translation="MEFNEFKDRAYFFQYINKGPYPDEEEKMKLYSCFCKIYNPSMKD
                     REILKATESKSGLTIIMRSSKIEYLPQTNHLVKIDRGLYSDKLFNIKEIRIDTPDIGY
                     NTVVLSEK"
     gene            complement(20391..20669)
                     /locus_tag="NBI57_000072"
     CDS             complement(20391..20669)
                     /locus_tag="NBI57_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HCD1821071.1"
                     /translation="MSLEEIKLWLRIDYNFENDLIEGLIQSAKSELLLSGVPDYDKDD
                     LEYPLFCTAIKYIIARDYESRGYSNDQSRSKVFNEKGLQKMILKLKKW"
     gene            complement(20738..21901)
                     /locus_tag="NBI57_000073"
     CDS             complement(20738..21901)
                     /locus_tag="NBI57_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HCD1821072.1"
                     /translation="MPTLYELKQSLGMIGQQLKNKNDELSQKATDPNIDMEDIKQLET
                     EKAGLQQRFNIVERQVQDIEEKEKAKVKDTGEAYQSLNDHEKMVKAKAEFYRHAILPN
                     EFEKPSMEAQRLLHALPTGNDSGGDKLLPKTLSKEIVSEPFAKNQLREKARLTNIKGL
                     EIPRVSYTLDDDDFITDVETAKELKLKGDTVKFTTNKFKVFAAISDTVIHGSDVDLVN
                     WVENALQSGLAAKERKDALAVSPKSGLEHMSFYNGSVKEVEGADMYDAIINALADLHE
                     DYRDNATIYMRYADYVKIISVLSNGTTNFFDTPAEKVFGKPVVFTDAAVKPIVGDFNY
                     FGINYDGTTYDTDKDVKKGEYLFVLTAWYDQQRTLDSAFRIAKAKENTGSLPS"
     gene            complement(21913..22686)
                     /locus_tag="NBI57_000074"
     CDS             complement(21913..22686)
                     /locus_tag="NBI57_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease ClpP"
                     /protein_id="HCD1821073.1"
                     /translation="MSMKAKYFQMKRKSKSKGEIFIYGDIVSDKWFESDVTATDFKNK
                     LDELGDISEIDVHINSSGGSVFEGHAIYNMLKMHPAKINIYVDALAASIASVIAMSGD
                     TIFMHKNSFLMIHNSWVMTVGNAEELRKTADLLDKTDAVSNSAYLDKAKNLDQEQLKQ
                     MLDAETWLTAEEALSFGLIDEVLGANEIAASISKEQYKRFENVPEDLKKDVDKITKID
                     DVDTSELVETPKESMSLEEKEKREKIKRECEILKMTMSY"
     gene            complement(22670..23908)
                     /locus_tag="NBI57_000075"
     CDS             complement(22670..23908)
                     /locus_tag="NBI57_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HCD1821074.1"
                     /translation="MNVIAKENIVTRIKKKLIDNWIDQSTSKLYDFSPWKNRSFWGVI
                     NNTLETNETIFSAITKLSNSMASLPLKMYEDYKVVNTEVSDLLTVSPNNSLSSFDFIN
                     QIETIRNEKGNAYVLIERDIYHQPSKLFLLNPNVVEMLIENQSRELYYSIHAATGNKL
                     IVHNMDMLHFKHIVASNMVQGISPIDVLKNTTDFDNAVRTFNLTEMQKPDSFMLKYGS
                     NVGKEKRQQVLEDFKQYYEENGGILFQEPGVEIEPLPKKYVSEDIVASENLTRERVAN
                     VFQLPSVFLNARSNTNFAKNEELNRFYLQHTLLPIVKQYEEEFNRKLLTKTDREKNRY
                     FKFNVKSYLRADSATQAEVYFKAVRSGYYTINDIREWEDLPPVEGGDKPLISGDLYPI
                     DTPLELRKSLKGGDKNVNES"
     gene            complement(23913..25604)
                     /locus_tag="NBI57_000076"
     CDS             complement(23913..25604)
                     /locus_tag="NBI57_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HCD1821075.1"
                     /translation="MTIKILNEPSPKLLTTWYAEQVIQGKIKTSKYVKKECERHIRYL
                     ENGGKWMFDEELAHRPIRFIEKFCKPSKGSKRQLVLQPWQHFIIGSLFGWVHKETKLR
                     RFKEALIFMGRKNGKTTTISGVANYAVSQDGENGAEIHLLANVMKQARILFDESKAMI
                     KASPKLRENFRPLRDEIHYDATISKIMPQASDSDKLDGLNTHMGIFDEIHEFKDYKLI
                     SVIKNSRAARLQPLLIYITTAGYQLDGPLVDMVEAGRDTLDQIIEDERTFYYLASLDD
                     DDDINDSSNWIKANPNLGVSIDLDEMKEEWEKAKRTPAERGDFITKRFNIFANNDEMS
                     FIDYPTLQKNNDIISLDELEGRPCTIGYDLSETEDFTAACATFALDNGKVAVLTHSWI
                     PKHKVEYSNEKIPYREWEEDGLLTIQDKPYIDYQDVFNWIIKMNEHYVVEKITYDRAN
                     AFKLNQELKNYGFETEETRQGALTLSPALKDLKEMFLDGKIIFNNNPLMKWYINNVQL
                     KLDRNGNWLPSKQSRYRKIDGFAAFLNTYTDIMNKVVSDSGEGNIEFISIKDIMR"
     gene            complement(25594..25899)
                     /locus_tag="NBI57_000077"
     CDS             complement(25594..25899)
                     /locus_tag="NBI57_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P27 family phage terminase small subunit"
                     /protein_id="HCD1821076.1"
                     /translation="MKLTKKQLKEYIEDYKKSDDMLINLYLETYEFYCRLRDELKNSD
                     LMMEHTNKAGASNIVKNPLSIELTKTVQTLNNLLKSMGLTAAQRKKIVQEEGGFGDY"
     gene            complement(26025..26345)
                     /locus_tag="NBI57_000078"
     CDS             complement(26025..26345)
                     /locus_tag="NBI57_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000160695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HCD1821077.1"
                     /translation="MTKQNNTYKHGRKSYRYDWFYHSKAWKKLREIALDRDNNLCQMC
                     LREGKITDANIVHHIVYVTEDFSKALDLENLMSVCYTCHNQIHANDKNKDKYKNKKFR
                     VEKI"
     gene            complement(26506..26943)
                     /locus_tag="NBI57_000079"
     CDS             complement(26506..26943)
                     /locus_tag="NBI57_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCD1821078.1"
                     /translation="MGKASYDIKPGTFKYIESEIYNLQENKKEINRLRMEILNPTKEL
                     DTNIVYGPLQKGEPVRTTELMATRLLTNKMLRNLEEMVEAVESEYLKLPEDHKKVIRL
                     KYWNRDKKLKIEQIGDACHMHRNTVTTIRKNFVKAVAYHAGIK"
     gene            complement(26956..28323)
                     /locus_tag="NBI57_000080"
     CDS             complement(26956..28323)
                     /locus_tag="NBI57_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HCD1821079.1"
                     /translation="MEHLAIDFKPHSYQKYAIDKVIDNEKYGLFLDMGLGKTVSTLTA
                     FSELQLLDTKKMLVIAPNQVAKDTWVDEVDKWNHLNHLKVSLVLGTPKERNDALNTEA
                     DIYVTNKENTKWLCDQYKKEWPFDMVVIDELSTFKSPKSQRFKSIKKKLPLINRFIGL
                     TGTPSPNSLQDLWAQVYLIDRGERLESSFSRYRERYFKPTHQVSEHIFKWELRDGSEE
                     KIYERIEDVCLSMKAKDYLNMPDRVDTKQTVVLSEKERKVYEELEKNYILESEEEGTV
                     VAQNGASLSQKLLQLSNGAVYTDDEDVRLIHDKKLDKLEEIIEESQGQPILLFYNFKH
                     DKERILQRFKEATTLEDSNYKERWNSGDIKMLIAHPASAGHGLNLQQGGHIIVWFGLT
                     WSLELYQQANARLYRQGQNHTTIIHHIMTDNTIDQRVYKALQNKELTQEELMKAIKAR
                     IAKHK"
     gene            complement(28304..28594)
                     /locus_tag="NBI57_000081"
     CDS             complement(28304..28594)
                     /locus_tag="NBI57_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VRR-NUC domain-containing protein"
                     /protein_id="HCD1821080.1"
                     /translation="MKESTLEKYLVKEITKLNGLCLKWVAPGTRGVPDRIIIMPEGKT
                     YFVEMKQEKGKLHPLQKYVHRQFENRDHTVYVLWNKEQVNTFIRMVGGTFGD"
     gene            28728..28832
                     /locus_tag="NBI57_000082"
     CDS             28728..28832
                     /locus_tag="NBI57_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1821081.1"
                     /translation="MNLMQHWKQTGFNLTARRERGVVSFVASMLHHQK"
     gene            complement(28935..31382)
                     /locus_tag="NBI57_000083"
     CDS             complement(28935..31382)
                     /locus_tag="NBI57_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000884880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence-associated E family protein"
                     /protein_id="HCD1821082.1"
                     /translation="MLDKVTQIETIKYDRDVSYSYAASRLSTHWTNHNMAWSDFMQKL
                     AQTVRTKEDLTEYNKMSKSEQADIKDVGGFVGGYLKEGKRRAGQVMNRSMLTLDIDYA
                     AQDMTDILSMFYDFAYCLYSTHKHREISPRLRLVIPLKRNVNADEYEAIGRKVADIVG
                     MDYFDDTTYQPHRLMYWPSTSNNAEFFFTYEDLPLLDPDKILNEYVDWTDTLEWPTSS
                     KEESKTKRLADKQGDPEEKPGIVGAFCRAYTIEEAISTFIPDLYEKHSTNRYTYHEGS
                     TAGGLVLYENNKFAYSHHNTDPVSGMLVNSFDLVRIHLYGAQDEDAKTDTPVNRLPSY
                     KAMQQRAQNDEVVKKQLINDKMSDAMQDFDEIENSDDAWSETLEITSKGTFKASIPNI
                     EIILRNDPNLKEKIAFNEFTKQIECLGKVPWNTNFKTRQWQDGDDSSLRSYIEKIYDI
                     HHSGKTKDAIISVAMQNAYHPVRDYLNKISWDGHKRLEKLFIKYLGVEDTEVNRTTTK
                     KALTAGIARVMEPGCKFDYMLTLYGPQGVGKSALLKKLGGAWFSDSLVSVTGKEAYEA
                     LQGVWLMEMAELAATRKAEVEAIKHFISKQVDRFRVAYGHYIEDFPRQCIFIGTTNKV
                     DFLRDETGGRRFWPMTVNPERVEVNWSKLTKEEIDQIWAEAKYYYEQGEELFLNPELE
                     EEMRSIQSKHTEESPYTGIIDEYLNTPIPSNWEDLTIFERRRFYQGDVDMLPTGNVDY
                     IERDKVCALEVFVECFGKDKGDSRGSTELRKISNVLRQLDNWSVYDGNKSGKIRFGKD
                     YGVQIAYVRDESLEDLI"
     gene            complement(31434..31634)
                     /locus_tag="NBI57_000084"
     CDS             complement(31434..31634)
                     /locus_tag="NBI57_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1514 family protein"
                     /protein_id="HCD1821083.1"
                     /translation="MWIVISIVLSIFLLILLSSISHKMKTIEALEYMNAYLFKQLVKN
                     NGVEGLEDYENEVERIRKRFKS"
     gene            complement(31702..31854)
                     /locus_tag="NBI57_000085"
     CDS             complement(31702..31854)
                     /locus_tag="NBI57_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000595263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional activator RinB"
                     /protein_id="HCD1821084.1"
                     /translation="MIKQILRLLFLLAMYELGKYVTEQVYIMMTANDDVEAPSDFEKI
                     RAEVSW"
     gene            complement(31851..32237)
                     /locus_tag="NBI57_000086"
     CDS             complement(31851..32237)
                     /locus_tag="NBI57_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000592218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1821085.1"
                     /translation="MIKKLKNMDGFDIFIAGILRLFGVIALMLVVISPIYTVASYQNK
                     EVHQGTITDKYNKRQDKEDKFYIVLDDKQVIENSDLFFKKKFDSADIQARLKVGDKVK
                     VKTIGYRIHFLNLYPVLYEVKKVDKQ"
     gene            complement(32234..32440)
                     /locus_tag="NBI57_000087"
     CDS             complement(32234..32440)
                     /locus_tag="NBI57_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1381 domain-containing protein"
                     /protein_id="HCD1821086.1"
                     /translation="MTQYLVTTFKDSTGRKHTHITKAKSNQRFTVVEAESKEEAKEKY
                     EKQVKRDAVIKVGQLFENIRECGK"
     gene            complement(32477..33010)
                     /locus_tag="NBI57_000088"
     CDS             complement(32477..33010)
                     /locus_tag="NBI57_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HCD1821087.1"
                     /translation="MNNLTVDQLKELLQIQKEFDDRIPTLNLRDSKIAYVVEFFEWFN
                     TLETFKNWKKKPGKPLDVQLDELADMLAFGLSIANQVGVSSEEIKEAIESSFKNTEFH
                     KMFNFKDKEFAQDAVVSTPQIIFKEFYPDQLAIVIVIDIAYNLYSIDQLIDAYKKKMK
                     RNHERQDGTADAGKGYV"
     gene            complement(33025..33186)
                     /locus_tag="NBI57_000089"
     CDS             complement(33025..33186)
                     /locus_tag="NBI57_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000889682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1821088.1"
                     /translation="MLEIIDQRDALLEEKYLNDDWWYELDYWLNKRKSESEQIDIDRV
                     LKFIEELKR"
     gene            complement(33187..33468)
                     /locus_tag="NBI57_000090"
     CDS             complement(33187..33468)
                     /locus_tag="NBI57_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000454993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1821089.1"
                     /translation="MEWKRLKNVVPHPVIKNKNLKSVYVTKDNVKEVQKELGFFEIFN
                     EEVLLTGFLSFQRIPIYIIWINPKSHKTPRYYFANEHEIERYFEFLEDE"
     gene            complement(33469..33642)
                     /locus_tag="NBI57_000091"
     CDS             complement(33469..33642)
                     /locus_tag="NBI57_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000028426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1821090.1"
                     /translation="MSISVGDKVYNHETNESLEIVQLVGDIRDTHYKLSDDSVISIID
                     FITKPIYLIKGDE"
     gene            complement(33632..33883)
                     /locus_tag="NBI57_000092"
     CDS             complement(33632..33883)
                     /locus_tag="NBI57_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016170782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1024 family protein"
                     /protein_id="HCD1821091.1"
                     /translation="MNNREQIEQSVISASAYNGNDTEGLLKEIEDVYKKAQAFDEILE
                     GLPNAMQDALKEDIYLDEAVGIMAGQVVYKYEEEQENEY"
     gene            complement(33876..34265)
                     /locus_tag="NBI57_000093"
     CDS             complement(33876..34265)
                     /locus_tag="NBI57_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000144704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HCD1821092.1"
                     /translation="MTFTLSDEQYKNLCTNFNKLLDKLHKALKDREEYKKQRDELIGD
                     IAKLRERNEELENMWRTVKNELLGRYEFYRFRLNELQIESRANKAVAINMGAKINASA
                     ILYRMDKLDGTNEFYEFLGQMEDDTNE"
     gene            complement(34262..34609)
                     /locus_tag="NBI57_000094"
     CDS             complement(34262..34609)
                     /locus_tag="NBI57_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1821093.1"
                     /translation="MMLKFKAWDKDKKVMSIIDEIDFNSGYILISTGYKSFNEVKLLQ
                     YTGFKDVHGVEIYEGDIVQDCYSREVSFIEFKEGAFYITFSNVTELLSENDDIIEIVG
                     NIFENEMLLEVMR"
     gene            complement(34606..35010)
                     /locus_tag="NBI57_000095"
     CDS             complement(34606..35010)
                     /locus_tag="NBI57_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1821094.1"
                     /translation="MKIKVKKEMRLDELIKWARENPDLSQGKIFFSTGFSDGFVRFHP
                     NTNKCSTSSFIPIDIPFIVDIEKEVTEETKFDRLLEVYEIQEGVYKSALHKGISLNER
                     FEDDNIFPTKAFYILNDDMTMTLIWKDGELLV"
     gene            complement(35013..35219)
                     /locus_tag="NBI57_000096"
     CDS             complement(35013..35219)
                     /locus_tag="NBI57_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1821095.1"
                     /translation="MKIKIEKEMNLPELIQWAWDNPKLSGNKRFYSNDVERNCFVTFH
                     VDSILCNVTGYVSINDKFTVQEEI"
     gene            complement(35233..35475)
                     /locus_tag="NBI57_000097"
     CDS             complement(35233..35475)
                     /locus_tag="NBI57_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1821096.1"
                     /translation="MTDSARKERLKHFFGSKRYLYQDNERVAHIHVVNGTYYFHGHIV
                     PGWQGVKKTFDTAEELETYIKQSDLEYEEQKQLTLF"
     gene            complement(35490..35690)
                     /locus_tag="NBI57_000098"
     CDS             complement(35490..35690)
                     /locus_tag="NBI57_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1821097.1"
                     /translation="MATQKQVEYVMSLQEQLELEDCEKYTDEQVKAMSHKEVSNVIEN
                     YKTSIRNEELYYECMSFGLPNC"
     gene            complement(35693..35950)
                     /locus_tag="NBI57_000099"
     CDS             complement(35693..35950)
                     /locus_tag="NBI57_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3310 domain-containing protein"
                     /protein_id="HCD1821098.1"
                     /translation="MSIISNRKVDMNKTQDNVKQPAHYTYGDIEIIDFIEQVTAQYPP
                     QLAFAIGNAIKYLSRAPLKNGHEDLAKAKFYVQRAFDLWEG"
     gene            complement(35950..36351)
                     /locus_tag="NBI57_000100"
     CDS             complement(35950..36351)
                     /locus_tag="NBI57_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PVL family protein"
                     /protein_id="HCD1821099.1"
                     /translation="MARRKVIRVRIKGKLMTLREVSEKYHISPELLRYRYKHKMRGDE
                     LLCGRKDSKSKDEVEYMKSQIKDEEKEREKIRKKAILNLYQRNVRAEYEEERKRRLRP
                     WLYDGTPQKHSRDPYWFDVTYNQMFKKWSEA"
     gene            complement(36351..36536)
                     /locus_tag="NBI57_000101"
     CDS             complement(36351..36536)
                     /locus_tag="NBI57_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3113 family protein"
                     /protein_id="HCD1821100.1"
                     /translation="MQHQAYINASVDIRIPTEVESVNYNQIDKEKENLADYLFNNPGE
                     LLKYNVINIKVLDLEVE"
     gene            complement(36549..38501)
                     /locus_tag="NBI57_000102"
     CDS             complement(36549..38501)
                     /locus_tag="NBI57_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase"
                     /protein_id="HCD1821101.1"
                     /translation="MNIDIETYSSNDISKCGAYKYTEAEDFEILIIAYSIDGGAISAI
                     DMTKVDNEPFHADYETFKIALFDPAVKKYAFNANFERTCLAKHFNKQMPPEEWICTMV
                     NSMRIGLPASLDKVGEVLRLQNQKDKAGKNLIRYFSIPCKPTKVNGGRTRNLPEHDLE
                     KWQQFIDYCIRDVEVEMTIAHKIKDFPVTAIEQAYWVFDQHINDRGIKLSKSLMLGAN
                     VLDKQSKEELLNQAKHITGLENPNSPTQLLAWLKDDQGLDIPNLQKKTVQEYLKEATG
                     KAKKMLEIRLQMSKTSVKKYNKMHDMMCSDERVRGLFQFYGAGTGRWAGRGVQLQNLT
                     KHYISDTELEIARDLIKEQRFDDLDLLLNVHPQDLLSQLVRTTFTAEEGNELAVSDFS
                     AIEARVIAWYAKEQWRLDVFNTHGKIYEASASQMFNVPVESITKGDPLRQKGKVSELA
                     LGYQGGAGALKAMGALEMGIEENELQGLVDSWRNANPNIVNFWKACQEAAINTVKSRK
                     THHTHGLRFYMKKGFLMIELPSGRALAYPKASVGENSWGSQVVEFMGLDLNRKWSKLK
                     TYGGKLVENIVQATARDLLAISIARLEASGFKIVGHVHDEVIVEIPRGSNGLKEIETI
                     MNKPVDWAKGLNLNSDGFTSPFYMKD"
     gene            complement(38570..39127)
                     /locus_tag="NBI57_000103"
     CDS             complement(38570..39127)
                     /locus_tag="NBI57_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2815 family protein"
                     /protein_id="HCD1821102.1"
                     /translation="MKAKVLNKTKVITGKVRASYAHIFEPHSMQEGQEAKYSISLIIP
                     KSDTSTIKAIEQAIEAAKEEGKVSKFGGKVPANLKLPLRDGDTEREDDVNYQDAYFIN
                     ASSKQAPGIIDQNKIRLTDSGTIVSGDYIRASINLFPFNTNGNKGIAVGLNNIQLVEK
                     GEPLGGASAAEDDFDELDTDDEDFL"
     gene            complement(39153..40319)
                     /locus_tag="NBI57_000104"
     CDS             complement(39153..40319)
                     /locus_tag="NBI57_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2800 domain-containing protein"
                     /protein_id="HCD1821103.1"
                     /translation="MKLDHSNRAHAKLSASGAKQWLNCPPSIKASEGIADKSTVFAEE
                     GTFAHELSELYFSLKYEGLTQFEFNKAFQNYKRNQYYSEELREYVEEYVANVEEKYNE
                     ALSRDNDVIALFETKLDLGKYVPESFGTGDVIIFSGGVLEIIDLKYGKGIEVSAIDNP
                     QLRLYGLGAYELLSLMYDIHTVRMTIIQPRIDNFSTEELPISRLLQWGTDFVKPLARL
                     AYNGEGEFKAGSHCRFCKIKHSCRTRAEYMQNVPQKPPHLLSDEEIAELLYKLPDIKK
                     WADEVEHYALDQAKGNDKNYPGWKLVEGRSRRMITDTKATLEKLVEAGYKPEDITETK
                     LLSITNLEKLIGKKAFSKIAEGFIEKPQGKLTLATESDKRPAIKQSAEDDFDKL"
     gene            complement(40316..40678)
                     /locus_tag="NBI57_000105"
     CDS             complement(40316..40678)
                     /locus_tag="NBI57_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1821104.1"
                     /translation="MAILEDIFEELKLLNKNLRVLNTELSTVDSSIVQEKVKEAPMPK
                     EETAQLETIEEVKETSTDLTKDYILSVGKEFLKKADTSDKKEFRNKLNELGADKLSTI
                     KEEHYEKIVDFMNARINA"
     gene            complement(40693..41016)
                     /locus_tag="NBI57_000106"
     CDS             complement(40693..41016)
                     /locus_tag="NBI57_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrmB family transcriptional regulator"
                     /protein_id="HCD1821105.1"
                     /translation="MTLQQKILSHFATYDNFNPDDVVEVFGVSKTHAKSTLSRLKKKG
                     KVEMESWGKWRVIEAQLHLTVVERKKEILEEQFELLARLNEQSDDPREIEDRIKLMIR
                     LANQF"
     gene            complement(41095..41256)
                     /locus_tag="NBI57_000107"
     CDS             complement(41095..41256)
                     /locus_tag="NBI57_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1270 family protein"
                     /protein_id="HCD1821106.1"
                     /translation="MSDTYKSYLVAVLCFTVLAIVLMPFLYFTTAWSIAGFASIATFI
                     FYKEYFYGE"
     gene            complement(41269..41532)
                     /locus_tag="NBI57_000108"
     CDS             complement(41269..41532)
                     /locus_tag="NBI57_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001124190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCD1821107.1"
                     /translation="MPKIIVPPTPENTYRGEEKFVKKLYAKPTEIHQLFGVSRSTVYN
                     WLKYYHEDDLGIKNLCIIYSPAGQLINIPKLEAYLIKRQEKIL"
     gene            complement(41557..41772)
                     /locus_tag="NBI57_000109"
     CDS             complement(41557..41772)
                     /locus_tag="NBI57_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1821108.1"
                     /translation="MNTRSEGLRIGVPQVSSKADASSSYLTEKERNLGAEILELIKKS
                     DYSYLEINKVFYALDRELQYRANNNKL"
     gene            41827..42192
                     /locus_tag="NBI57_000110"
     CDS             41827..42192
                     /locus_tag="NBI57_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1821109.1"
                     /translation="MPLISDEFDTLTKDQQYILSVLYKDYLECVKLGSVKLTCNNFGS
                     AKDIHTKYFQKLHFEDVKYDLNKLKNSGFLNGVYASNTIYHVTISDKTVVYFENEFKN
                     NLKSIIDSISKIASIIPGL"
     gene            complement(42161..42406)
                     /locus_tag="NBI57_000111"
     CDS             complement(42161..42406)
                     /locus_tag="NBI57_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HCD1821110.1"
                     /translation="MNIQEATKIATKNLVSMTRKDWKESHRTKILPTNDSFLQCIISN
                     SDGTNLIRYWQPSADDLMANDWEVINPTRDQELLKQF"
     gene            42462..42671
                     /locus_tag="NBI57_000112"
     CDS             42462..42671
                     /locus_tag="NBI57_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001560888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1821111.1"
                     /translation="MKACLYLSNDKFVEIDNLEKVIKSGHRGTVEISKEKIKSSLFTN
                     GSYTFVGDKIVAIASAKIEFIEFID"
     gene            complement(42661..42804)
                     /locus_tag="NBI57_000113"
     CDS             complement(42661..42804)
                     /locus_tag="NBI57_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000939501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1821112.1"
                     /translation="MLQKFRIAKEKSKLKLNLLKHANSNLETRNNPELLRAVAELLKE
                     INR"
     gene            complement(42833..43609)
                     /locus_tag="NBI57_000114"
     CDS             complement(42833..43609)
                     /locus_tag="NBI57_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001148544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HCD1821113.1"
                     /translation="MQALQIVEQNETHYVDSREVAEMIGKRHDNLVRDIKGYIKVLED
                     SSKLSSHNFFEESTYVNSQNKVQPCYLLTKKGCDIVANKMTGSKGILFTATYVDAFHK
                     MDEYIKQQAQLNVPQTPMQALEMMFKAQKDQEQFNKQMQQEITGIRHIVGIETKNWRN
                     DTNKMLSAIAQHLGGGAMHQKVKSEAYKALEEKGRCNLKIRMQNRKGKMLANGATKTQ
                     INKLSKLDVITDEPRLVEIYISVIKSMAIKYGVDISQFEI"
     gene            complement(43623..43859)
                     /locus_tag="NBI57_000115"
     CDS             complement(43623..43859)
                     /locus_tag="NBI57_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002469095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCD1821114.1"
                     /translation="MPIDAKLLKSKMALKEHNIKTLSEEIGVNRDTLSNMIHGRTKPS
                     YPVINGIYFALELTPQEGRDIFFNEDLRKKKVLT"
     gene            44011..44325
                     /locus_tag="NBI57_000116"
     CDS             44011..44325
                     /locus_tag="NBI57_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012991010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCD1821115.1"
                     /translation="MCFSKRMKQSREKQGMTLAELGRKIGKTEATVQRYESGNIKNLK
                     NDTIESIATALNVNPAYLMGWVEENDDEVQHRAAHLEGELTDDEWQRVLDYADYIRSK
                     RK"
     gene            44338..44799
                     /locus_tag="NBI57_000117"
     CDS             44338..44799
                     /locus_tag="NBI57_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000525002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin"
                     /protein_id="HCD1821116.1"
                     /translation="MGLYEETLIQHDYIEIREADVLPDNLDGVWLGDLILIKRGLSDR
                     EKAGILFEELAHNKLTYGDIADYSKFNNRKFENYARRHGFISAVPLREIVEAYNYGVR
                     NLYELSEYLQLSEEYILEAIEQYKKIYGIGTHYGEYSITFEPLRVFKYKEI"
     gene            44817..45401
                     /locus_tag="NBI57_000118"
     CDS             44817..45401
                     /locus_tag="NBI57_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001636973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1821117.1"
                     /translation="MKRLLGLTLASALVLGACGSHDGDKKEESKKTETKKDNKDKKKE
                     TKEKAEAKKENANQNDNNNQVNNENNTNVNNDQQTNRPLTKDEISQRVKNGHNVNGMV
                     DADGNTWYQAQGAGDVIGYTKPDGTQCTVGGCVTPQQQEQINEANYKEMEKYGYSREK
                     YDAIQKEASKLQQQKENGEITAEEFTNRYIELYD"
BASE COUNT        12936 a         9010 c         6311 g        17164 t
ORIGIN      
        1 atggcatctt tgtacaaagg tagacttgca ttaaatacaa tgccatgatc ttcgcaatgc
       61 acataaactt ctacaccatc atctttacct acaacatttg tagctttaac ttttagtcca
      121 aagttatctt taaagaattg ttcacctact ttttcaaatt ctttacgatg cttcttcgca
      181 aattcaatcg catctttttc tgcaggtggt tggaagcctt gtcctacata ttttgaagct
      241 tccatttctt ctggtactga ttttgtttca ttgttggatt cgttattggt agttgaacat
      301 cctgatagta ttatcgttgc tattaagatt agtttaattc ttttaaacat agcccatctc
      361 ccatttatgt gtttgtttag aatatagaga aattagctga tttcaagtca ttcgacctca
      421 atccttatag actcattatc actgtaatta actcgattag tgctaatagt agattttgct
      481 agttgtaaaa atattttcgt attttcaaaa ttaaggtgta actttttcgt actttcagac
      541 aaatctaaaa catcatctaa ttttttgtcc tttgaaaagt tactacttct cgaaaacaat
      601 gttgtatgta cttctattgc agctttatag cctactcctt tccttgcttg tttcatacct
      661 tttttaaaat tcagattatt tttctttatt aggggttcgt aatatttcct atattcttgg
      721 agcgtcggta tattagcaac tatataaaaa tattcatttt catatccact attttgtgtt
      781 ttgttaattg cctctttagt aaatcctgta tattggtatt tcttttcatt ttcttttaaa
      841 aatttatata aattatcata cttttctttt tgcgctcgat attcaaagcc actcaacact
      901 gtaccaacta aagtactcat atcatcgcct ttatcctcac ttcttaatga gctatcactc
      961 tcaattattg atttgtcaaa tggaatactt gcattaaata cgatgtcgtg gtcatcacaa
     1021 tgcacgaata cttctacacc gtcgccacta cctacaacat ttgtagcttt aacttttagt
     1081 ccaaagttat ccataaaaaa ttgttcgcca cgtttagcaa ttttatcttt atgcttcttc
     1141 gaaaattcaa tcgcatcttt ttctgcaggt ggttggaagc cttgtcctac atattttgaa
     1201 gcttccattt cttctggcac tgattttgtt tcattgttgg attcgttatt ggtagttgaa
     1261 catcctgata gtagtatcgt tgctattaag attagttttg ttcttttaaa catagctcat
     1321 cacccattta tgtgtttgta aaacttttat gttaaaaaaa ctacttattc tcaaagaaaa
     1381 caagtagcat ttaataaatt acttagtata cagctagttt ttctaattgt tctttaactt
     1441 gaattaagtt tgaccgtatt agagaggcag attgatccat cgtttgaatt gcttgtcctt
     1501 cattttcgtt caagccatta caaacaactt caaactgttg tgccatttga tcaagacgtg
     1561 catgagcttg tgtgtttaaa ataaacatat catcataatg tgatggtaaa gcggtcattc
     1621 tctaacgtac acgagcgtcc aaaaacactg ttgtatcaat gtttttgtca tttttaacct
     1681 tttagatttt ccacaagcgt ttgccctttt tcaaataatc tgcccttttt tcgccccgaa
     1741 aaaacgcaaa aaataaccac aatcctaaat taataggatc tgtggttttg ttggttgtag
     1801 gggaataaat ataaccgtat cgattaagat acggttgtag cgaatgtaac atttctatgt
     1861 tgttaagata tatgtatcga gtgatgacaa ggaagatgtc tcctgtggga ccaacagtca
     1921 gatacatggc ctctgcccgg ctatatagtt cactcctact atataaaagt aagtataaca
     1981 taaaaagcac cccgtaaact gttatacggg aatgctaaag tcatatatac tacggggagt
     2041 agtatgaaaa ctatgctctc tatcgcaagg aaaaaacacc cagtgacatg cttgggtgaa
     2101 caaggataga tgtaaatagt tgatgcatgt gtaacacatc ataacaaaaa actagcccga
     2161 aggctagcta taacataaaa aaataggcaa gtaccgaagt acctgccagt tacgcacatt
     2221 taaatcttga gagtaatgtt aaaaagtgta taggaatatt aacatccatc caaatagtta
     2281 tttaataact gtaagattcc ctataattaa tgtagcaaaa tttttattct aagtaaatac
     2341 taaatcgtgc taaacttacc aaaactactt attctattac ctgccttgtc tacctctccg
     2401 gtcgcaatat agcgacgttg tccactatta gcaatataag taatccatct ataaccatta
     2461 atacaatatg caccgtcata cgtaattgtt gtgttgttgg gtaatacccc tgtaattctt
     2521 gaattagttg aataaccgtc tcttacatta ttacctttaa cattagctac tgtgtaataa
     2581 ccagtttctt ttttatacgg tacattattt ttatcgggtg tataacctgc tggcactggt
     2641 ggattttttt ggtttttagc tgatgtttta acattaccag ctaccaaacc acctataggc
     2701 ttaccatgaa tcgcaccagc tattaattta gaatacaagt catagttttt cttaatccaa
     2761 tccatatctt ttttattagt aataaaacct aattcagata aacgataatt catatttatt
     2821 tctgctgata cattaacgtt tagtaaatca ttacgaggtg ttacacctct tatttgtcct
     2881 aagttatttt taataacatc ttgtatactt ttatcaatag tatctgcatt aaattgactt
     2941 gagataataa catgtccacc acttgcactt tctcccgctg cgtctaaatg aatctctaga
     3001 acaatgtcat acccctgtga tttaacccaa tataagccat aatctttttt attgcctaca
     3061 ttaacaccgt atgcagtatc ttgatacatg tcttgtgatt gacttgagcc accatataat
     3121 gcaacttcat gaccggcatg tcttaaatac ttagcgatat ttggcgttat atatttacgg
     3181 ataaaatcgc gttcgtttgt tccgtttcct actgctccag gatcgttata accatgaccg
     3241 gctacaagca taattttttt aggtttaatt actgcttgct ttttggcagt tgcttgctta
     3301 ataacgcttt tagctttatc tccaacactt actttatctg ggaaatttaa tctaataaaa
     3361 tacattgggt catcgtaata atgaacatgt cttgtaacag tttcaggacc ccaaccaggt
     3421 tgcgcaacgc catttgtcca acctttacca ttccaatttt gcccatatga tgtgaaagtg
     3481 tttaaatttg cgctctcaac aatttcaaca tgtccagctc cgccaccata ctttgacggg
     3541 aaaacgacaa tatccaactt ttgcggtaaa aagctatcat agtttttaat tatttgcccg
     3601 tatttttcaa tccttgcttt attatcaaat ggaatattat aagcgtataa accttgtaac
     3661 ctttcgcctg ttgctatcat aaaaaacata tttgcgtaat cgtaacactg aaatccataa
     3721 aacaaatcag gattgaactg cttccctaat gaattatcaa accatttttc tgcttggttt
     3781 tttgttatca acattggtca tcacctaccc taaatcattt gtgtcgttca tattcgtagg
     3841 tgtcattact tctttaattg gcgcttgccc tgttgctttt ctatatttac tttcagcttt
     3901 atatttcttt aatttttgat tcgcccattt cccttcttga gatgttggat tatctttata
     3961 tgtagtatat aaagcaacaa ctgttaagat aatcgatgaa acactttctt catctactgg
     4021 tatcggactt atacctttat tcgctaagaa ttgatttact aatgctaaga tcaatacgat
     4081 gtatcttgtt attacttttg catccatttg tttgctcctt ttatccaaaa taaaaagcca
     4141 gtgccgaagc actgactctt aaatattact tacacttacc aaaccagaaa cacgaccaaa
     4201 agctatatcc taaaattccc ttaagcatgg tgatcacctc ctttaaatgc caaaaatagt
     4261 ttttaacaag gctataacaa atgtacttag aatcgtccct attaatccta gaatccacat
     4321 cttgatgtct ctaatatttt tagcattttt ctctttattt ttttcatctt cttctttgtc
     4381 acgccttagt tcttcgaaat ttctatctaa cttgtcataa attttttctt gcgttctcag
     4441 actgtcttct attctgtcga atttttcaaa catagtctta tcattttctt ctaatcgcgt
     4501 taaacgccaa tcttgttcgt gtcgtttggt aaatccaaac attacaccac ccactttatt
     4561 caaattaaaa agccataaga ttataaccta tgactctaga ttttctggat acttttctcc
     4621 tgtaataatt gcatattcct ctttatctat aacttccata tctacatacc acgctatatc
     4681 ttctttacta tattctttca attgatacca tgttttaata tcttcgaatg ttggtgaaat
     4741 taatttaagc attttcagtc tctcctttaa cctcttctaa ttttttatta agtgtcacaa
     4801 gttgttttgc cattagtgca ttttgcttat taacttgcat cgataacttt gtactttgaa
     4861 caacttgttt ctgcatacta gcaaccattt ttcgtaagat gtcatcagaa gcgactgtgt
     4921 tttgttcttc actgtcaatc tgttgatgca agtcatcttt ttcttctgaa taatcttcgt
     4981 taaaaactat ttccccattt gaatatttaa aggctttagg tctaaaaact tgagagaaat
     5041 tttctggtaa attttcaata tcaatacctt cttcaaagcc gccaatgata gcgtatgaaa
     5101 ttatctcatt acgcttgtta actaatattt gcattatttt ctcactccta taattttgtt
     5161 aattgtccct ctatttgcgt tcgcaccaga gcctctttga cttcctaagt cgaaatagac
     5221 atcgtttgat atagttaaag atgtacgact agatttagtt aatccaaact cataaacacc
     5281 tccaccattt ccatcaccat ctggaagatt tgagggattc aatgaaatct ttcctcctcc
     5341 aaaaggactg ccaaactctg taaagtcacc acctggaaaa gtcccataaa aaattaataa
     5401 aataaattgg tctaaactct catttaagta caatgtagag cccacaccat ttgctgttcc
     5461 atcaaaaata accgaatacc ttttattaaa cttgtcatct gcgtataatt tagcgttact
     5521 ttcggccata ttagcttttg attgggcact ttgaacagtt tcaaaaggtg tattgtaatc
     5581 attaatagct aattctgacc actcagacca tgaacccgct tcttttcttt taacaaacac
     5641 tttatttgta ccgttcggtc gataagtcat acgcttgtaa tctgaagtta ctactaaata
     5701 ttcgacagta ccgttagtac taacatctct tggataattt atagcttgcg aaacataaat
     5761 aaattgggtt gaatcaccta ttctttgttc tggattatta aaatcaaatc cagtaatctg
     5821 cattatctta ccatcatctt tagtaatctt agctttttgc caatttgaag tagaaccact
     5881 tgtgactaaa ccaccactat tcactgactg cttgaaggct tcatgtttct catccatata
     5941 tcgcttttgc tcatcgaatg ttcttgaata tgcttgcgct ttattttcca aatcagatat
     6001 acggctatta gcaagttgct ttaattcatc aatacttgaa gattttgcta tttgaatatc
     6061 tgatagacct ttttctttag ctttttcaat cagactcgca taatcctcac cattttttat
     6121 agcctcgtcc attgctttcg cgcgatccat aatagttttt tctaattctt gaaactcaac
     6181 aatatagtgt agttttgttt cagagggaat cttgctaaac aaactttttt caacgttaaa
     6241 tgtgatagtt ctctctacaa ctaccacgtc tgaattacct aattctgcaa ccgaaacttg
     6301 agcttgataa cttccatctc ttttaattac atcattaggt aattgaaatt ttaaaatacc
     6361 tttaaatgga tctaatattt ctagtggagc aactaccatt actcctttac ctcgaatcgc
     6421 tattcgtgct ttgatatttt cttcactcag taataacggt tgattatttt taatgatatt
     6481 aaaaagaaga acagaagaat cactctctcc tgttctaaaa gttatatcta gatttgaaat
     6541 attttcataa tgcgctgtgt tttctaaatt tatagctaca gatttctcta aattactcat
     6601 taacttataa ttctcccttc gtgtaaagtc catggccctg aacttgtttt actatcataa
     6661 tttttcaata gtatctcagc agatgctgta acactattac gaactagcct atgaacaaag
     6721 ccacctgtgt ttgaagcttc tacatataag ttccaaccag ctaccccttt acgttcagtt
     6781 ggaaaatctg taaaacgttt tgtatcatcc gtagttaaat aaaacgacat gcctactatg
     6841 ttaatatctg acatttttgt gatgaatgaa ggtactctct cccatttacc actattttta
     6901 ggcacataat tccagtccga aatgtctcca gttcttccag aaagcaccct ttcaaaagtc
     6961 atcatattcc ttgcataact attacgcgtc aatatctgaa ttacatcacc gccagtttgt
     7021 ggtggcttaa cttccaagaa ccaacctgca tcacgccatt ctcttggtaa tgggaaatca
     7081 tcgatttgaa ctgtatgatc agtgtataaa tagtaaagac ctggctctgt taacatccca
     7141 agattcttaa gtttatcagg cctcattggt aaaggtttaa ctctaccacc tgtgtcactc
     7201 atgataaaag gaacgcctct tgagtgaagt atttctaaaa tacctctttg cccaatcatg
     7261 aaaatacgat gtgttctatt tccatcacca ccgacagtaa cacctagcat caaagctttt
     7321 ttaccactat ctttgtcata gtatatttgc aaaccttctg cttccgcaaa ttcgccagga
     7381 aatgaatcta gtgttccacc atagtcagca ttaacctgat acgcttcttc tcctgtttct
     7441 aaatcgaaag ccgttaaata gtttctatta tttggattac tgtctcctgt ataccaatac
     7501 aagtattttt catcaaaagt cacaccctgc attggttggg tttcgtttgt tagtctcata
     7561 gggatactga ttttatgcaa aactttatca atatttttat caacatcgtc taaacttctt
     7621 atctctatat aattcattga gttttcaagt tcccactgac ttctaggtct ctcaattctg
     7681 tatagaattt tattttcttt ttcatttatg acaggggtga tgtagggttt ttctgggtgt
     7741 cctgtaaata catcttgcat accatacttg ccatagctaa tttccacatt aggcgtatac
     7801 ttgaaacgaa ctaatgtatt ctcattatta ccatttaaga taaaactata aatccataac
     7861 tcatcatcaa tatatctata accgttatgt gtaccatgac ccccacctac aatcaatgag
     7921 ctgtctataa attgaccatt aggtcttaaa cgacttagca tatagccatt atttctagct
     7981 tgtgtcatgt atactatgcc tgttctatta tcaaaccaga aggattgcat tactgcattt
     8041 gtaagaggtg caagttctgt cacaaataaa aactcttgct tatcaggttc aaaacgatac
     8101 tcgatatcaa gaatttcttg tttggtctta tttaattctc ttatagtttc ctctttatta
     8161 atttgagttt tggtttccca atcgtctaaa tgttctttta atgtgtcaaa ggtttcgccg
     8221 tttacattaa ctcgagcttg aacaatctca ttagcactgt tattacgtgg tgccacaaca
     8281 agtgcgttaa tttgactttg taaagatttg tttactgctg cttgcgatct accattataa
     8341 taaatttgct cagcgaagtg ttgcattgtt ttagctttct gatgcaactt aaactctgtt
     8401 gtcaagccaa gcgcaaattg ctctattctt tgcaggtttt gaatttcttt agctctataa
     8461 tctcgacctg ctaaagctcc caaatccttt attaaataca aattttccat aatgcacctt
     8521 cctttctaat aaaatagcac tgtaccaagt ttcccactat cgtcaactgt tattttccac
     8581 aatttaccgt ttggggattt ctgtacaatg ctattttgaa taattcctgc ttcgcctatt
     8641 tttaatttat ctaatttatt tttatcatct accgaaatga taccgtcttg aggcaatcca
     8701 tcaatatcac tactgcctgc ataaggtatc ccatttatag ctttccagtg tgtagctgga
     8761 aagtactgtt tatcgttttc aagtagcgct ttgattttaa cttcttctgt tgccattata
     8821 ttaatacact ccctatatcc attgtctcga aaggagaatt caaagtacta gtgtataaat
     8881 gatttatacg atttgcttga tagttatatc tattatcttg tgcaataact cgtctgttaa
     8941 gtgcttgttg aatttgtacc atatctttta tttcattgct gaaagacact tcatctattg
     9001 cgtttacaaa tggatgtgac ctatcaagtt taacaacctt taattcagtg ttatatccca
     9061 ttaattcatg aacaaaaaat acgctatctc ttggctctat tttttcataa cctatataat
     9121 taacatctaa ttcagtctta ggagtatcat ttatttgctt ttttgcaaat tctaacagct
     9181 tatcctgtgt ttcgatatct tcatttgttt gcgtattagc atatcgaatc ccaaactgct
     9241 ttgcactatc tgcgacgtag tcgacaattg ctttgtattg attgcgacct gaattatcag
     9301 caattaaatt taagactgtt gatttttcag ttccaacata catacaaggc ttagcttttt
     9361 tatttgaaga tatatcaatt ctatttttgg ggtcttctcc taaaaatatc atttctaaaa
     9421 cgtgcttgcc tttatcaata ttttttatta aatctattgt ttcagactga accgacttag
     9481 caaaacaaga aatttgctta atttgcttgc cgtctaaaat caacttatat attccacctt
     9541 gagagccctt ttttattgta aatctaactg tttcattacc atacttgcaa tcaaagttaa
     9601 tagtagcttt agacccaatt gtttcggtac gataagtacc ttcttttata aaaccatttg
     9661 aatatttaat gtcagttgtt ctaataggat tataattttt cttttcctca gctgtatact
     9721 tttttccaaa aacttttata gctgttctta attccaatgt actgacagtt gcagatacag
     9781 tatcagtatt atattgatat cgaatcactt tctcgcttct ttgataaaat gtttcaggag
     9841 aataaaaacc aatctctgta tcatttgggt aaattataca gccaaacagg tctaccgctt
     9901 ctttacagta ttctaagccg tttttgttac ctaattcgtc aatcggtact tttcgcttaa
     9961 aatctccaat tattttatag gtcattttga ccgaagtttt ttgatttgca aatccatatc
    10021 ttaagtactc atctaaagag tattctggcg ttttaccagt ttcgctactg tcgtcatcaa
    10081 gcttatttga ttccactgag tgattttgaa attcatacat tatgtgatat gccgtaactt
    10141 caataaaaac tttatcacct tcaacctttg gcgctgtctg cttaattgta tatttttcac
    10201 catgataaat tatgaagttt tcacaaatca ataaatcaaa aacaaaacta ttatgagtag
    10261 ttctataaac tgtaaaggtg atgtacctag cttcattcag ttcataatat tctttaaaag
    10321 aaccataatc tacatctagt aaattttcac aaatcaattc attaaaatcc attactgata
    10381 aatgatcatg ataatccatt aaatcaccta cctataaata aaaggaaact taaatgtagt
    10441 tttaatatca ctgacgtctc ctttaatctt aaattcattt ttacctggcg ctaatgttat
    10501 aatgcctcta tttgtatcaa ttcccactct atttatatct cgatatgcat acacaccatc
    10561 taaaacaaaa tcagtgtttt tatctatact tttgttgtac ttaaaaatat cacctgttgt
    10621 atagttaacc agttcaaatc ctccactcgc atttaaatta attaatattt tcaaatcgtg
    10681 cttgaatcgt ggatttatcg tatcagtaga accgttccaa atagtaaatt gatttgatgt
    10741 atgagtatat ttaggtgtga aatcaagagg aattccattt tcaaacatcc aattagagtc
    10801 gaataagaac tcgctatcgg tccaattaac tgattcagaa tatcctttat aaacatttaa
    10861 acttacttca atttcagttg aagaaccatc ttttaaatta gatgtaacat tagctgtatt
    10921 cactgcgtat tttacaccag gcatttgaga agtaataaca taataaggat gtctacgatt
    10981 aaatacagat ctaaaccaat gctcaaataa atttaaatct ataacatcta taccatcata
    11041 gccaaacctt aatactaatg aaaaaggcgc aaaactaatt gcgcccggta aaataccatc
    11101 tactccgtta atagttacac tattatcatt ggtgtttgga ctttcagccc ttgcatctaa
    11161 aaatataagc tgattaaaat ctgttattac ttcttccttg taaccatcta tgatttttac
    11221 aaaagattgc attaattagt caaacctccc atataattat ttgcatttgc tctatgtcca
    11281 ctttgttttg acaatacctt ttctaaatct ctaattgcat cactagagct taaattatta
    11341 tcctgagaag aaacagtttg aatcaatgca tctgttaatt tatttccttt atccgttagc
    11401 ataacaattt gtttcaacaa tttttcaacc gtagaagtat cattatttac agtgatgtta
    11461 ttttgcttgc catccatacc gatgatgcgc ataacctgtt cagttaattg cattgctctt
    11521 ttacgtcgtg tcaacggaat aaccatttct ggtttatctc cttctcccac ttcagcaatt
    11581 tgatgtttag ttataagacc tccattcgca taggcataat ctcctgcgcg tttaaagcct
    11641 ccccaaccgt acttagcaac aatgtatttc atagctgaaa tcgcctcatc agtaggatta
    11701 taaatatttc catgtcctgg cttagcaaaa gctctaaatg aaggttcaat catttggaac
    11761 atacctttag acggtgtacc tgctcttgcg ttggaatccc agttgttaat agcatctgcc
    11821 tgatagttac tttcacgctt ggctactctt aacatctgag tagtgatata gtcagatcta
    11881 tatcgtccac ctaaaattga ttgtgctctt agaatagctc tttttgcatt ctcgtatccg
    11941 cttccgccga ccttgccacc gccaccttta agtgacttca accatttttc tggatcttta
    12001 gctgtatcat tccctggatg cgacccttgc atcaattgga aatgtaagtg tgctcctcta
    12061 acgaaattac ctgtagcacc tgatttccct atcagttgac cagctttaat acgttggcct
    12121 tgtcttgcta attgcttaga taaatgcata taccagttcc attcgttagc accggtctta
    12181 atttgtatag aattaccgcc accgtaatca gtccatactt tatcagctat accgccttta
    12241 acagcataaa tgttcgttcc agtaggcatt tgaaagtcga taccatagtg acggccgcca
    12301 ttaaagttaa gtccacctgt gtagctccca aacctttgcc aaattggatg gtcaaataga
    12361 tagcttccat cgcctccacc accaaaatct tcaaaccacg attttacttt gtctactaat
    12421 ttctttttga gcaatgagta cgcgccttta gcaattttta ctgtagcgtt agctccgcct
    12481 ccaaaattaa tatttaaacc tgacattact ttatttacta gtttccctgg atgttgtaca
    12541 taatcccaca catcgccgat tttatcgcct aaccaagatg caccatcttt gattttatcg
    12601 cctgccgctt caaccatttc ttctgcacct tttttgatat tatgcgctgt gtttttagct
    12661 gtagctccaa attctcctgc ttttttacct atattacctt taagttggtc tagccaatct
    12721 ttctttttcg taccaccatg gaattttggc aaaacaccca tccgctgtaa cttcagagtg
    12781 tcattggcat ttattacact atctccaact cctagtggaa caaccacatc tcgtccttgg
    12841 ggtgcatgga atgttccgtc agccctgtga attacttctt gaactccacc acctggggcg
    12901 tttccagaac ctctatcatt taatacagca aatgtcggtt gcgttaatgc tcccgaatta
    12961 tcggtagcta caccctttcc tgctaaagta ccagtagaca atgtaggtat tggcttgatg
    13021 agatttttat cagtaatggc tttagatatt ttattaatac cgccaatcat gctattcaaa
    13081 ccgccaatag ctttattagc aacattttta cctaaatcag ccgcagctct tcccatgtct
    13141 ttaccaatat ctctaatcca atcatatgtt ttggatagcc attttctaaa accattaaat
    13201 actgatttag cgttagacca tgccgaactt gaaattgcat caaaacgatc gtgggctctt
    13261 gaatacatat ccccagtcca atcttttaaa gatttgtatg agttactaaa ccatttcgat
    13321 gttcctttcc aaacggattt tgcattcgac caagctgtac tagaaatatt atcccatttc
    13381 gcgcgagatt tattagccat atccgttagc cagccctttg cacttttata tgcattgcta
    13441 aaccattttg atgtgcctct ccaaatagat tttgaatgcg cccaagcttt atctgaagca
    13501 tctgaatact tttgcttagt ttgattgtaa atacttcctg ttgtcgattt aacagattgc
    13561 caagcttttc caaaccattt accagtacta ttagctatag ccttagtgtg gtatcctaca
    13621 gaacttttgg ctgagctcca acctgaactt aatttgcttg gaatcccttt gattccgctc
    13681 cacatttttt tcatttcgcc gccaaaatga ttagcatttc tgcccatttt actaaaggct
    13741 tcgccagttt tactttttac gccgtcccaa gcatttccaa accatttctt tatattttct
    13801 ctgtttctac gagctgtttc ttcttgttct ttagcgtatt tatcgctttt tttcttttgg
    13861 tcttctctaa agttagacca ccaactttta aggccattcc accacttttc agtattttta
    13921 tatacacgtc cactggataa atccatttct ttatcaatat ctttattttg ctttttaaca
    13981 acatctacta cagcatcttt tttagatttt gcttttctta cttcatcttt atgtctttga
    14041 tcagcaatag ctaacaattt atctttttca gacttagaaa ggttgacgtt attttttata
    14101 gcaatgacat catcttcata ttgcttgtcc acttcttttt ttcttgcttt tcttgctttt
    14161 tctgcttctt taattgcttt gctcgcttcg tctattgaat aagcatttct gtttctttgc
    14221 attcttacta aaatacgctc ttgctctttt tcagtcttac tcaattcttt aacagtgata
    14281 tcacgtcttt gattttcaag cttttcaatt tcttttcttt cattttctga aatctgacca
    14341 tcactcaaag ctttttcttt caattctttg attttctgat tgagttcttg ctctttttta
    14401 attcgcaagt catttttttc tttagttcga gttaaaatgt tttgcttttc ttgttcatcg
    14461 aatgcactat acttatcaat aagttcttga gttttttcga gttccttttt atttcttttt
    14521 tctatttcag ctataaggtt attagataaa tccgtttcaa ttttcaaaag tttttttgct
    14581 ttgtcttctg atatctgacc cgagtttaaa cgtacttttt ccatgattct gctattttct
    14641 tcagaataat gcacatattt ttctaaagct ttttctgttt cttttgaaac acctttccct
    14701 aacactttta cagtatcaga cgctttttta gaagctgtgc ccatggtttg cataaatcct
    14761 ttaaacttgt tgactcctac tttgagaagg tcatcgtcgc ttaatgattt ataaccatct
    14821 ttcatatctt ttgaaaactt ttctttgaag cttttgccga tacttccaag atagttttta
    14881 aactctccta gctttctaac agcgccgcca ataattttac caccaaaaaa ctttatagtt
    14941 tctcctaaac cgttaatacc gtttctgaac cattccacac gatcatatgc ggttttaaaa
    15001 actttatacg caattgtaat agcagttatt gtagcaccta taggtcctgt taaaaacctt
    15061 aaggctacac ccgcaaatct tgcgcctcca cttactgcaa ataaagattt tgcagctaat
    15121 cctaaaccgt ttttcaaaag tttgaacggt aaaattgcta gctttgcaga atttttcaaa
    15181 acatttatag gttttaaatt aaacatcata gctccggcta accctttaaa gccttttgac
    15241 gtttttcctg ttgtagaacc aagaaataat gtttgaagac ctaaagattt cattgctttt
    15301 gaattagtat ttgaaaggat tgtattttca gcaatacgtc tatttaatga cgcatatcct
    15361 ttagcagcac ttccaactgt acgtattaat aaccctccag caagaactgc aggtccaatt
    15421 gctgcaccaa aaagtgctaa tcctactgaa gcttttctaa cccaaccagg gagatgtgta
    15481 aatccatcaa ctaattttgt taaaccttcc gctcctgctc taatcatagg cgttaaatct
    15541 ttaccgactt cgattgctaa tgattcaaaa gcgccaccta attgttccag agcgcctttg
    15601 agattatctt tcatcaaatc tgctgctttt ttactttcgc cattggaatt ctttaaggat
    15661 ttactatagc tattaatttt atctggtccc gcttcaatca aggctaaaaa tccacttgct
    15721 gcttcagtac caactattgt agccactgta gctagttttt gttctctcgt catgcctttc
    15781 atattatctt ggaactgtct aatcaattca cccatgccaa caaattgacc tttagcatca
    15841 gacaaatgaa tacctaattt tttcatttcc ttagctgtat ttttacttgg attagctagc
    15901 ctgataaatg aagctcttag ggcagtacct gcttgagaac cctctaaacc tgagttagat
    15961 aaaacttcaa ttgctgcgga agtgtcctct attgaaactc ctaatgcttt tgcaggagta
    16021 ccagcatact tcaatgcatc tcccatgtac tgaatatctg cagcactatc atttgctgat
    16081 ctcgcaagta aatcagcaac atgatttgca tcagatgctt ttaaaccgaa agagttaatc
    16141 gctgaagcca ttacagttgc agttgtagcc atttctgcac cacttgcttc tgctgcactg
    16201 ataacacctg gcatagcctc cattgtttgt ttggcattaa agcctaaagc tgccaattct
    16261 tccatacctt tagcaacttc gttagcactt ttactggttt tagctcctaa gtcaactgct
    16321 tgattagaca tgcttttcaa gtctttactg cttgcttgcg caatcgctcc aactcgagac
    16381 atttggcctt caaagtctgc acttgttttt aatgctgcac ctaaccctaa agtaattggt
    16441 gtagatacgc ccatcgtcat tgtacgtccc agggaagtca ttttgtctcc aatagaacta
    16501 aatttctttg acatgacatc cgcttgactt gcaagtttac cgaaatgact ttgagctatc
    16561 atttgttctt tattaaaagt cttcatttcg gatgaagctt tatctattga acgctccaaa
    16621 ttatttaaag cagctttttc tttattaaca gctgtttcag cttttgcgac attagcgcta
    16681 tgattcttaa tagtattgtt taaatcatta aattcttttt ctgtttgctt taatttagta
    16741 ttagttttag cgtaagaact ttcaatttta tcatttgatt ttgaaagatt gtcattttgc
    16801 acttttagtt tttgaacttg attgccttct tgtttatatt gttcaacaag tgctttatgc
    16861 ttagcggact gcttctgtac tgcgtcactt gctcttttta gttgtgcagt agtagcttgg
    16921 ttactattct taagcttttg ttctgcatct ctcaaatgtt taagtttttg atacgcatct
    16981 tgtttacgtt gatttgtacg tttatattga ttttcagctt ttttaagttc tgtattcgat
    17041 gattttaagg cttctttaga tttatcaaga gctaattttt cttttttatt ggcttctact
    17101 aactttaaat atgctttctc aacatctttt acactggatt tagctttttg gtaattagcg
    17161 ttaacttgtt taagctcatc ttctacttga gaatacatct ttttttgaac tttaagccta
    17221 tcatttaacc ccttaattct cgcctgatat ttttccattg atttttcaga cttatcaaat
    17281 gctgacagat tagctttcat ttcactatta acaacaccta attgtcgctt taaacctttc
    17341 atgccttctt ggacacctaa atggtctaat ttcagctcca aggtcatgcc ttctactttt
    17401 tcattcatat taacctcctt tctagcttcc aaaaagtttt cttaaatccg tacctgtaat
    17461 gactttttgt tcactttgtt tttcttcagt ctcttcttta ttctcttcat taagtatttc
    17521 taaaagtttt acatacggct gttttctgac ttcagttaat gtccacccat actgctccat
    17581 acagaaacgt tgtattttct taatgttcga taaaatgtct tttattgaga ttgttcttct
    17641 gtctttccca tctcttctgg ttcagtttct gaatcttctt catcttcacc attgatttct
    17701 cgaaatatat cttgtaaggc ttttgtataa gttttagtac tcatcttgtt cagaacatct
    17761 tcttcagtca atccttcatc tttaaataaa tctactaata actgtcgctc tttttgtctc
    17821 atttttgttg cgttaggtgc ttctttttta ttctcttgat ttactaattc taaatactca
    17881 tagcattttt ctgcttcgcc cattgttaca tcttcttttg tatagctctc tgtttttcct
    17941 gttttacgat ctttaatttc aaatttaatc attgtattag ctccttttat tcaaataaaa
    18001 aagacgcaga tatactgcgc cttaaatccc tatccgtttg ttactgtcac tgaaatttgt
    18061 cctgacttat cgcttccatc agtagacata gcagtgatta ctgaagtacc ttcagctaca
    18121 ccgtgaattg ctcctgtatt ttcatctaca gtaacaaatt ctggatgttc acttgtatat
    18181 ttcaatattt tattcgttgc tgtgcttggt gcaatgtttg gctcaacatt gtcatcggta
    18241 tttaccataa ttgatttagt ttctggtgta aatgatacgc ctgagactag aattggattg
    18301 gttttgaatt gaggtacatc aactttacta gattctttac cattttcttc ccatgccact
    18361 tggtaagtac cttttggata agttgtatcc gcttctaaat tagataaagt tactgacact
    18421 ttgccttcac cttgttcaga agctacgacg tcgtctcctt tataaacctt taaagtttta
    18481 gtcataaatt attctccttt gatttatttt gaaagcccct attctgctga aactgttgca
    18541 gattttgaat taactgctac ttcaacattt tggggattag ctgggtaacg aacctgcaga
    18601 atcctctgaa tgatcttcac tgtccgtgta tccaacgaat acttttttga agaattctgc
    18661 ttctccttct ttaccttcat gataaccgta tacaatacct tgtgacgttc catcaacatc
    18721 aacttttcta ttcatccagt cacctgttaa ttttgtaggt tctggggctt ctgctttttc
    18781 tcctcgtgtt ttaaattcaa ttgaatccaa gctaaaagta cctttaagta gcgcaacgta
    18841 cactggttga cctgttaaac catcttcaga ttctccaata actgttacgt atggtgctct
    18901 tgtattctct cctacccaag atgtaccatt tttatcttta gaacgaccaa taactgtatt
    18961 tagatcttca cttggaatat taaaaatact catgtcagac ttaacttcat tagtaccttg
    19021 ttttttcatc caaacacgtt tgttagatgc aaacatatct actaaatctg gtgctaaacc
    19081 tgtgatattt agatcaactg taccaccttt ttcatcttcc catgtcatgc gtttaactac
    19141 ttttgttgct tctgggttaa aaactccaac gtataatctt ttaaaaccta ctttataaga
    19201 accttgtcct tctgccattg cttatttcct ccttaaaaat taaaaagcac acctattcga
    19261 tgcgctgatt tttataatat atattttttg gtatgccttg ataacgtctc gacatcacat
    19321 aacgtttagt ttcttcaaaa taagcatcta actgactaga tgcttgaatt aaattttgtt
    19381 gatataacag atatcttatt cgttttgtta tatcaattgt tttctgatta tttgaagatt
    19441 ctacatctat ttgaattaag tattcttcac taagatattt atcagacata aagtctgaag
    19501 gtaaatcata aataggtgta ataacaacaa aaggtttgga agtttcagca ttttcagtga
    19561 ctttataata gtatattcta gaatttatat atgtttggag ctctgcatca gataataaaa
    19621 tttcttttat ggtgtttaat atattcattt atctggccaa ctcctttttt ataatttctc
    19681 tatacttccg ttcattagca gctaatgttt ttgcaataac tccaaaacct cttggtgtat
    19741 atttttttcc atctcttgta taaccatgtt cattcaagtg aataatgttt ttgcgattca
    19801 tagggcctac ccattcaatt aaaacagctc tttcttgact tcctactttt gtataaggct
    19861 tagatttagt catttcttct atgctagcac ccgtatcttt aaaactctcg aattctttct
    19921 ttaaagcctt tataaaaaat tcagatgctt catttaaagc tctatcactc ttagcttgca
    19981 ttgattgttt accgtatacc gattctaatt tcttcaacac ttcaggtatc cctttaattt
    20041 ctacactcat ttttctgata aaaccactgt attatagcca atatctggtg tatcaattct
    20101 tatttcttta atgttgaata atttatcgga atataagcct ctgtcaattt taactaagtg
    20161 atttgtttgt ggtagatatt caattttaga agacctcata attatggtta gtcctgactt
    20221 tgattcagtc gcttttaaaa tttctctatc tttcatagaa ggattataaa ttttacaaaa
    20281 gcaactatac aatttcattt tttcctcttc atctggatat ggtcctttgt ttatatattg
    20341 aaaaaaatac gcgcgatctt taaattcatt aaattccatt taaaaatcac ctaccacttt
    20401 tttaatttca aaatcatttt ttgcaatcct ttttcattaa acaccttgct tctagattgg
    20461 tcatttgagt atccacgact ttcataatct cttgcaatga tatatttaat cgctgtacaa
    20521 aaaagcgggt attccaagtc atctttgtca taatctggaa ccccacttaa taataattca
    20581 gacttagccg attgaatgag accttcaatt aaatcatttt cgaaattata gtcaattctc
    20641 aaccacaatt taatttcttc caaactcatt tcatcacccc tattcggctg atattacagc
    20701 tgatttagcc ttagctgtta cattaacctt ttggggttta gctgggtaat gaacctgtat
    20761 tttcttttgc ttttgcaatt ctgaatgcac tgtctaatgt acgttgctga tcataccatg
    20821 ctgttaatac aaacaaatat tcgccttttt taacatcttt atcagtgtca taagttgttc
    20881 catcatagtt aattccaaaa taattgaaat ctcccacaat aggtttaact gctgcatctg
    20941 taaatactac tggtttgcca aatacttttt ctgctggtgt gtcaaagaaa tttgttgttc
    21001 catttgaaag aacactaata attttgacat aatctgcata tcgcatataa attgttgcgt
    21061 tatcacggta atcttcatgt aaatctgcta aagcgttaat aatagcatca tacatgtctg
    21121 ctccctcaac ttctttaaca gatccattat aaaatgacat gtgttctaat ccagatttag
    21181 gacttactgc taaggcatct ttacgctctt tagctgctaa tcctgattgt agtgcgtttt
    21241 caacccagtt tactaaatct acatctgatc catgaattac agtatctgaa attgcagcaa
    21301 atactttgaa tttattagta gtgaacttga ctgtatcacc ttttaatttt aattcttttg
    21361 ctgtttctac gtctgtaatg aaatcatcat cgtctaaagt gtatgaaact cttggaatct
    21421 ctaaaccttt aatgttagtt agacgagctt tttcacgtaa ttggttttta gcaaatggtt
    21481 ctgaaacaat ttctttagaa agtgtttttg gtaagagctt atctccacct gaatcattac
    21541 ctgttggtaa agcgtgtaat aaacgttgtg cctccattga aggtttttca aattcatttg
    21601 gtaaaatcgc gtgacgataa aactctgcct tagctttaac catcttctca tgatcattta
    21661 aagattgata agcttctcct gtgtctttaa ctttcgcttt ttctttttct tcaatgtctt
    21721 gtacttgtct ttcaacaatg ttaaatcttt gttgtaagcc tgctttttct gtttctagtt
    21781 gtttgatgtc ttccatatca atatttggat ctgttgcttt ctgactcaac tcatcatttt
    21841 tattttttaa ttgttgtcca atcataccta atgattgttt taattcatat aatgtcggca
    21901 tttcatttcc tcctaataac tcattgtcat ttttaaaatt tcgcattcgc gtttaatttt
    21961 ttctcttttt tctttttctt ctagtgacat actttcttta ggtgtttcaa ccaattcaga
    22021 tgtatctaca tcatcaattt ttgtgatttt gtctacatct ttctttaaat cttccgggac
    22081 gttctcgaaa cgcttatact gctctttaga gatactagca gctatttcat tagctcctaa
    22141 aacttcatct attaagccaa aagataaggc ttcttcagca gtaagccatg tttctgcatc
    22201 taacatctgc tttaattgtt cttgatctaa gttttttgct ttatccaaat acgctgaatt
    22261 actaacagca tctgttttat caagtaaatc cgctgtcttt cttaattctt ctgcattacc
    22321 tacagtcata acccatgaat tatgaatcat taaaaaacta tttttgtgca taaaaatagt
    22381 gtcaccactc atagcgataa cactagcaat tgatgccgct aaggcatcga catagatatt
    22441 aatttttgca ggatgcattt ttagcatatt gtatattgca tgcccttcaa atacactgcc
    22501 tccagatgaa tttatatgaa catctatttc actgatgtct cctagttcat ctagtttatt
    22561 tttgaaatct gtagcagtta catcactttc aaaccattta tcacttacaa tatcaccata
    22621 aataaatatt tcacctttac tttttgattt tcttttcatt tgaaaatact tagctttcat
    22681 tgacattttt atcaccacct ttcaaagatt ttcttaattc aagtggcgtg tcaattgggt
    22741 ataaatcacc gcttattagc ggcttatctc caccttcaac tggtggtaaa tcttcccact
    22801 ctctaatgtc atttatagtg tagtaaccac tacgaactgc tttaaagtac acttctgctt
    22861 gtgttgcact atcagccctt aaataagatt taacgttaaa tttaaaatac ctattttttt
    22921 ctctgtctgt tttagtaagt agtttccgat taaattcttc ttcatactgt ttgacgattg
    22981 gcaataaggt atgctgcaag taaaatctgt ttaactcttc atttttcgcg aaatttgtat
    23041 ttgatcttgc atttaagaat actgagggca attgaaaaac gttagctact ctttctcttg
    23101 ttaaattctc gcttgccact atatcttcag agacatattt tttaggtaac ggttcgattt
    23161 caacaccagg ctcttggaat aatattccac cgttttcttc atagtactgt ttgaaatctt
    23221 ctaacacttg ctgccttttt tctttaccta cattggaacc atatttaagc atgaaagaat
    23281 caggtttttg catttctgta agattaaagg ttcttactgc attatcaaaa tcagttgtat
    23341 tcttcaacac atcaatcgga ctaatgcctt gcaccatatt agatgccacg atgtgtttaa
    23401 aatgcaacat gtccatatta tgaacaatca atttatttcc agttgcagca tgaatggaat
    23461 aataaagttc acgtgattgg ttttcaatta acatttcaac aacatttgga tttaataaga
    23521 aaagctttga tggttgatga tagatgtctc gttcaattag cacatatgca ttaccttttt
    23581 catttctgat tgtttcaatt tgattaataa aatcaaaact gctcagagaa ttattcggtg
    23641 acactgtaag taaatcagat acttctgtat taactacttt ataatcttca tacattttca
    23701 agggcaaact agccatcgaa ttagataact ttgtaatagc tgaaaatatc gtttcattag
    23761 tttcaagcgt attattaatt acaccccaaa aagatctatt tttccatggg ctaaagtcat
    23821 aaagcttaga agttgactga tcaatccaat tgtctatcaa ttttttcttt atgcgtgtga
    23881 caatattctc ttttgcgata acattcacct ccttaacgca ttatgtcttt aatactaata
    23941 aactctatgt ttccttcacc actatcagaa acaactttat tcataatatc tgtatatgtg
    24001 tttaaaaatg ctgcaaaacc atctatttta cgatatctgc tttgcttaga cggcaaccag
    24061 tttccgtttc tgtctagttt caactgaaca ttattgatat accatttcat taaaggatta
    24121 ttattaaata ttattttgcc gtctaaaaac atctctttta gatctttcag tgctggactt
    24181 aaagtcaaag ccccttgtct tgtttcttct gtttcaaatc cataattttt taactcttga
    24241 tttagtttga aagcattggc tctatcataa gtaatttttt ctacaacata atgctcattc
    24301 atctttatta tccaattaaa gacatcctga taatcaatat acggcttatc ttgaatagtt
    24361 agtaagccat cttcttccca ttctctatat ggtatttttt catttgagta ctctacctta
    24421 tgcttaggaa tccaagaatg tgttaagaca gcaactttac cattatctaa tgcaaatgtt
    24481 gcacatgcgg ctgtaaaatc ttctgtttct gataaatcgt aaccaatcgt acaaggtctt
    24541 ccttccagct catctaagga aataatgtca ttattttttt gaagcgttgg ataatcaatg
    24601 aagctcattt cgtcgttatt agcaaatata ttaaatcttt ttgttataaa atctcctcgt
    24661 tcagctggtg tacgtttagc tttttcccac tcttctttca tttcatctaa atcgatagaa
    24721 acgcccaggt taggatttgc tttaatccaa ttcgacgaat catttatatc atcgtcatca
    24781 tctaaagatg ctaaatagta aaaagttctt tcatcttcga tgatttgatc taatgtgtct
    24841 cttcccgctt ctaccatatc aacgagtgga ccatctaatt ggtaacccgc tgtcgtaatg
    24901 tagataagaa gaggttgtaa ccttgccgct cttgagtttt ttataactga aatcaattta
    24961 tagtctttaa attcatggat ttcatcgaaa atgcccatat gtgtatttaa accatccaac
    25021 ttatcactgt ctgaagcctg tggcataatt ttagatatcg ttgcatcgta atgaatttca
    25081 tctcttaaag gcctgaaatt ctctctaagt tttgggctag cttttatcat agccttagat
    25141 tcatcaaata gaatcctcgc ttgtttcatc acattagcta acaaatgaat ttctgcacca
    25201 ttttctccat cttgtgacac agcatagtta gcgacaccag aaatggttgt tgttttacca
    25261 ttttttcgac ccataaatat taaagcttct ttaaacctgc gcagctttgt ttctttatga
    25321 acccagccaa ataaactacc aataataaaa tgttgccaag gctgtaatac aagttgacgt
    25381 ttagatcctt tagagggttt acaaaatttc tctataaatc gaataggtcg atgtgctaat
    25441 tcttcatcaa acatccattt accgccattt tccaaatacc taatgtgtct ttcacattct
    25501 ttttttacat atttacttgt ttttattttc ccttgaataa cttgttctgc ataccacgtt
    25561 gttagtaatt ttggtgaggg ttcatttaat attttaatag tcaccaaatc caccttcttc
    25621 ttgcactatc tttttccttt gtgctgcagt taaacccata gacttgagta agttatttag
    25681 tgtttgaact gtttttgtca gttcaatact taatggattc ttaacaatat tacttgctcc
    25741 agctttattg gtatgctcca tcatcaaatc actgtttttc aattcatccc gtaatcgaca
    25801 ataaaattca tatgtttcta aatataaatt aattaacata tcgtcagatt ttttgtagtc
    25861 ttcaatatat tctttcagct gtttttttgt taatttcata agaaaacccc ctttcatgaa
    25921 aaattatccg cgctgctctc gttcctgcct tcaccggttc ccggtgtaaa atggaattcc
    25981 ccaccaggta ggggggatta aaaattattt tataattctt ttatttaaat cttttcaact
    26041 cgaaactttt tatttttata tttatctttg tttttatcat ttgcatgaat ttggttatga
    26101 caggtatagc aaactgacat caaattttct aaatctaaag ctttactgaa gtcttctgtt
    26161 acatacacaa tgtgatgcac tatgtttgca tctgtaattt tcccttcacg caagcacatt
    26221 tggcataggt tgttatccct atctagtgct atttctctta gctttttcca tgctttagaa
    26281 tgatagaacc aatcatatcg gtaagactta cgtccatgct tataggtgtt gttttgtttc
    26341 gtcattactc acacctcact aatcgtacaa caaaagacac accacaaagt gatgtgcctc
    26401 atatacatgt ttcgtctatt atcgtaagga gcctatacat ctttttgtcc cacactaaca
    26461 tattagcaca gatttgtagg ccttttgcac aatctttgca caatgttatt tgatacctgc
    26521 atgatacgct accgctttaa caaagttctt tcgtatagta gtaactgtat tacgatgcat
    26581 gtgacatgca tctcctattt gctctatctt tagcttctta tctctattcc aatactttag
    26641 cctaattact ttcttatgat cttcaggtaa ctttaagtat tcactttcaa ctgcttcgac
    26701 catttcttct aggtttcgta acatcttatt agtcaataac cttgttgcca ttagttcagt
    26761 tgttctaact ggttcacctt tttgcaacgg tccatacaca atgttagtgt ctagctcttt
    26821 cgttgggtta agtatctcca ttctcaatct atttatctct ttcttgttct cttgtaggtt
    26881 atatatctct gactcaatat atttaaatgt acctggctta atatcgtatg atgcctttcc
    26941 catcttatac ctccattact tatgcttagc tattcttgct ttaatagctt tcatcaattc
    27001 ttcttgtgtt agttctttat tttgtaaagc tttatatact ctttgatcta tcgtgttatc
    27061 ggtcataata tgatgaataa tagtcgtatg attttgtcct tgtctgtata atctagcatt
    27121 tgcttgttga tataattcca atgaccacgt aagtccaaac caaacaataa tgtgtccacc
    27181 ttgttgtaag tttaatccat gccctgcact tgctggatgt gctataagca tcttaatgtc
    27241 gccactattc caacgttctt tatagtttga atcctctaat gtggttgctt ccttaaacct
    27301 ttgaagtatt ctttctttat cgtgtttgaa gttataaaac aatagtattg gttggccttg
    27361 agactcctct ataatttcct ctaacttatc taacttcttg tcatgtataa gtcttacatc
    27421 ttcatcatct gtataaactg caccattaga tagttgaagt agtttctgac ttaatgatgc
    27481 cccattttga gctacaactg ttccttcttc ttccgattct aaaatatagt ttttttctaa
    27541 ttcttcatat acttttcttt ctttttcaga taagactact gtttgttttg tatcaactct
    27601 gtcaggcata ttcaaataat cttttgcttt catgctcaaa catacatctt ctattcgttc
    27661 atatatcttt tcttcagagc catctcttag ctcccactta aaaatatgtt cgctaacttg
    27721 atgagttggt ttaaagtacc tttctcgata acgactgaat gaagactcaa gtctttcacc
    27781 tctgtctatc aaataaactt gagcccataa atcctgtaaa ctatttggac taggtgttcc
    27841 tgttaatcct ataaatctat taatgagtgg taatttcttt tttatagatt taaacctttg
    27901 actcttagga cttttaaatg tagacagttc atcaattaca accatatcaa atggccattc
    27961 ttttttatat tgatcgcata accatttagt attttcttta ttggttacat agatatcagc
    28021 ctctgtgttt aatgcatcat ttctttcttt aggtgttcct aagactaaag acactttcag
    28081 atgatttaaa tggttccact tatcaacttc atcaacccat gtatctttag caacttgatt
    28141 aggtgctatg actaacattt ttttagtgtc taacaactgc aattcactaa atgctgtaag
    28201 tgttgatact gttttcccta gccccatatc taaaaacaaa ccgtatttct cattatcaat
    28261 cactttatct attgcatact tttgatagct atgtggtttg aaatcaatcg ccaaatgttc
    28321 cacctaccat tcttataaaa gtatttactt gttctttatt ccataacaca tacactgtat
    28381 gatctctgtt ttcaaattgc cgatgcacat atttttgtaa aggatgtaac tttccctttt
    28441 cttgcttcat ttctacaaaa tatgtttttc cttctggcat aataataatt ctatctggta
    28501 cacctcttgt tccaggtgcg acccatttta aacataatcc atttaacttt gttatctctt
    28561 tcactaaata tttttctaat gtcgattctt tcatatattc accttgtata caaaatttat
    28621 atttgtgttc cgatgttgca tcaattcttg ccaaactttt aaaaatagct gttagagggt
    28681 tatccctata cccctttact ccctaacact actttttaaa ctttatagtg aatttgatgc
    28741 aacattggaa acaaacaggg tttaacctta cagcgagaag ggaaagaggt gttgtatcat
    28801 ttgttgcatc aatgttgcat caccaaaaat gatacaacac ctaagattac tttttacacc
    28861 accgtgttgc atcactcaaa aaatgatgca acatttgata caacatctaa aaatgtatat
    28921 ttattcaata tttcttatat taaatcctct aaactttcat ctcttacgta cgctatctgt
    28981 acaccataat cttttccgaa tcgtattttt ccacttttat tgccgtcata tacagaccaa
    29041 ttgtctaatt gtcttaagac attagaaatc tttctaagtt ccgtagatcc tctgctatct
    29101 cccttatctt taccaaaaca ttcaacaaac acttcaagcg cacagacctt gtctctttca
    29161 atgtaatcta catttcctgt tggtaacata tcaacatcac cttgataaaa tcgtcttctt
    29221 tcaaagatag ttaagtcttc ccaattgctt gggattggcg tgttaagata ttcatcaata
    29281 atacctgtat atggagattc ctcagtatgt ttactttgga ttgaacgcat ttcttcttct
    29341 agttcagggt taaggaacaa ctcttctcct tgttcataat agtatttagc ttctgcccag
    29401 atttggtcga tctcttcttt ggttagttta gaccagttca cttcaactct ctctggattt
    29461 acagtcattg gccaaaaacg tcttccacca gtttcatctc ttaagaaatc aactttatta
    29521 gttgtaccaa tgaaaataca ttgccttgga aaatcttcaa tataatgtcc ataagcaaca
    29581 cgaaaccggt caacttgttt agatatgaaa tgcttaatag cttcaacttc agcttttctt
    29641 gtagctgcaa gttctgccat ttccattaac caaacgcctt gtaatgcctc atatgcttcc
    29701 ttaccagtaa cagaaactaa actgtcagaa aaccatgcac cacctaattt ttttagcaaa
    29761 gcagatttac ctacaccttg aggaccataa agtgtaagca tatagtcaaa tttacatcct
    29821 ggctccatta ctcgagcgat tccagcagtc aatgcctttt tggtagttgt tctattcact
    29881 tcagtgtctt caacacctaa gtatttgata aataactttt caagacgttt atgtccatcc
    29941 cacgatattt tatttagata atctcttact ggatgataag cattttgcat tgctacgctt
    30001 ataatggcat cttttgtttt gcctgaatgg tgtatgtcat aaatcttttc gatataactt
    30061 cttaaactgc tatcatcacc gtcttgccat tgacgtgtct taaaattagt attccatggc
    30121 actttcccta aacattcaat ttgtttcgta aattcgttaa aggctatttt ttcttttaaa
    30181 tttggatcat tacgcaatat aatttctata tttgggatac tagctttgaa agtacctttc
    30241 gaagtaattt ctaacgtctc agaccatgca tcatcgctat tttctatttc atcgaaatcc
    30301 tgcattgcat cagacatttt gtcattaatt aattgctttt taacaacctc atcattttgc
    30361 gctctttgct gcattgcttt ataactaggt agtcgattaa ccggagtatc tgttttagcg
    30421 tcttcatctt gagcaccata taagtgtatg cgtactaaat caaaactgtt cacaagcata
    30481 ccgctaacgg gatccgtatt atgatgagaa taggcaaact tgttattttc gtataacacc
    30541 aatccacctg cagttgaacc ttcatgatag gtataacggt tagtagaatg tttttcgtat
    30601 aagtcaggaa taaaagttga tatagcttct tctatcgtat aggctctaca aaatgcgcca
    30661 acaattcccg gcttttcttc tgggtcacct tgcttatctg ctaatctttt agtcttactc
    30721 tcttcctttg aagacgttgg ccattctaat gtgtcagtcc aatcaacata ttcatttaat
    30781 attttatctg gatctaacaa aggtaaatct tcataggtaa agaaaaattc tgcattattg
    30841 ctagttgaag gccaatacat taacctatgt ggttgataag ttgtatcatc gaagtaatcc
    30901 atgccaacga tatctgcgac tttacgccca atagcttcat actcatctgc atttacattt
    30961 cgttttaaag gaatcactaa acgcagtctt ggacttatct ctctatgctt atgtgttgaa
    31021 tataaacaat atgcaaaatc ataaaacata gataatatgt cagtcatatc ttgagcagca
    31081 taatcgatat caagtgttag cattgaacga ttcatgactt gaccagcacg tcgtttgcct
    31141 tcttttaaat atccaccgac aaatccgcca acatctttta tatctgcttg ttcagactta
    31201 gacattttat tgtactcagt taaatcttct ttagttctaa ctgtttgtgc tagcttctgc
    31261 ataaagtcag accaagccat attatgatta gtccaatgtg tggataaacg actagcagca
    31321 taagaatatg agacatcacg atcatattta attgtttcta tttgagtgac tttgtctaac
    31381 atgttcggct cctttcatta ttttagatag agcagagaag ccaacgcctc tctttagctt
    31441 ttgaatcttt ttctaattcg ttcaacttca ttttcataat cttctaaacc ttcaacacca
    31501 ttatttttta ctaactgctt gaaaagataa gcattcatat actccaatgc ttctatggtt
    31561 ttcatcttat gagaaatgct acttaacaag atcaataaaa atatagataa aacaattgaa
    31621 atgacaatcc acatatttac aacacctcca gtgctattgc taaacacatt aatataatta
    31681 attcaaaaat gataatatct attaccatga aacttcagct ctgatttttt caaagtcact
    31741 tggcgcctct acatcatcat tagccgtcat cataatatat acttgctcag ttacatactt
    31801 acctagctca tacatcgcta gtaagaataa tagtcttaat atttgtttaa tcattgttta
    31861 tctaccttct ttacttcgta taagaccgga tataaattta aaaagtgtat tctatatcca
    31921 atcgtcttaa cttttacttt atcacctact tttaacctag cttgtatgtc tgcgctatca
    31981 aacttctttt tgaagaataa gtctgagttt tcgattactt gtttatcatc taatacaata
    32041 tagaatttgt cctctttatc ttgtcttttg ttatatttat ctgtaattgt cccttgatgt
    32101 acttctttgt tttggtaact agccactgtg tatataggcg atatgacaac aagcatcagt
    32161 gcgattacgc cgaataatcg cagtattcca gcaataaaga tgtcgaaccc atccatattt
    32221 ttaagttttt taatcatttc ccacactccc ttatattttc aaacaactga cccactttaa
    32281 taactgcatc ccttttaact tgtttctcgt acttctcttt cgcttcttct ttactctctg
    32341 cctcaacaac tgtaaacctt tgattactct tagctttagt tatgtgtgta tgtttacgtc
    32401 ctgttgaatc tttgaatgtt gtgactaagt attgtgtcac ttccccaaaa cctccttgac
    32461 tcgatctaag atgtctttac acgtatcctt ttcctgcgtc tgctgttcca tcttgtcttt
    32521 catgattcct tttcattttc tttttgtatg cgtcaatgag ttggtcgata gaatataagt
    32581 tgtaagctat gtctatcact attacaattg ccaattggtc gggataaaat tctttgaata
    32641 ttatctgtgg tgtactaaca actgcgtctt gagcaaattc tttatcttta aaattaaaca
    32701 ttttgtgaaa ttctgtattt ttaaaacttg attcaatcgc ttcttttatc tcttctgatg
    32761 acactcctac ttgattcgca atactcaatc caaacgccaa catgtcagct aattcatcaa
    32821 gttgtacgtc taacggctta cctggtttct tcttccagtt cttaaacgtt tccaatgtat
    32881 taaaccattc aaagaattca actacatatg ctattttgct atctcgtaag ttcagcgtcg
    32941 gtattctatc gtcgaactcc ttttgtattt gtaaaagttc ttttaattga tctactgtta
    33001 aattattcat ttattcgtta tctcctatcg ttttaattcc tcaataaatt taagcactct
    33061 atcaatatca atctgttcac tttctgactt gcgtttattc aaccaataat ctaactcgta
    33121 ccaccagtcg tcgtttaaat acttttcttc tagcaatgca tcacgttggt cgatgatttc
    33181 aagcatttac tcgtcctcca aaaattcaaa atatctttca atctcatgct cgttagcaaa
    33241 gtaatatcta ggcgtcttat gagatttagg attaatccaa ataatgtaaa taggtatcct
    33301 ttgaaatgat aaaaatccag ttaataacac ttcttcatta aaaatttcaa agaaacctaa
    33361 ttctttttga acctctttca cattatcttt tgttacgtat accgacttta agtttttatt
    33421 tttgataact gggtgcggca ccacattttt taatcgtttc cattccactc actcgtcccc
    33481 cttaattaga taaattggtt tagtaataaa atctataatg ctaataactg aatcatcgga
    33541 cagtttataa tgtgtatctc taatatctcc gaccaattgc acaatctcta gactttcgtt
    33601 tgtttcatgg ttatatactt tatctcctac actaatactc attttcctgt tcctcctcat
    33661 atttatagac aacttgacct gccataatcc ctactgcttc atcaagataa atatcttctt
    33721 tgagtgcatc ttgcatagca ttaggtaaac cctcaagtat ttcatcaaac gcttgtgctt
    33781 tcttatacac gtcctcaatc tcttttagta atccctctgt gtcattgccg ttatacgcac
    33841 tagcacttat aacggactgt tctatttgtt cacggttatt cattagtgtc atcctccatt
    33901 tgtcctaaaa attcgtagaa ctcatttgtt ccgtctaatt tgtccattcg gtacaatata
    33961 gcacttgcgt tgattttagc tcccatgttt atagctactg ccttgttcgc tctactctca
    34021 atctgtagtt cgttaagtct aaaacggtaa aattcgtatc ttccaagcaa ttcatttttg
    34081 actgtgcgcc acatgttctc cagctcttcg ttacgctctc ttaacttagc tatatctcca
    34141 ataagctcat cacgttgctt cttgtactct tcacgatctt ttaatgcttt gtgaagttta
    34201 tctaataact tgttaaagtt agtacaaaga tttttatatt gttcatctga taaggtgaac
    34261 gtcatctcat aacctccaat agcatctcat tttcaaaaat atttccaaca atttcaataa
    34321 tatcgtcatt ttcacttagt aattcagtta cattgctaaa agttatataa aaggctcctt
    34381 ctttaaactc gataaaactt acttctctcg aataacaatc ttgaacaata tccccttcat
    34441 aaatctccac accgtgcaca tctttaaatc ctgtgtattg taatagtttt acttcattga
    34501 aacttttata acctgttgaa atcaaaatgt acccactatt aaaatcgatt tcgtcaataa
    34561 tactcataac ttttttatct ttatcccaag ctttaaattt caacatcata ctagcaactc
    34621 cccatctttc caaatcaatg tcatcgtcat gtcatcgttt aagatataga atgctttagt
    34681 aggaaaaata ttgtcgtctt caaaacgttc gttcaaactg atacctttgt gtaatgcgga
    34741 tttatagact ccttcttgaa tctcatatac ctctaacaac ctatcaaact tagtctcttc
    34801 cgttacttct ttttcaatat caactatgaa ggggatatca attggaataa aacttgacgt
    34861 cgaacactta tttgtatttg gatgaaaacg aacgaatcca tcactaaatc ctgttgaaaa
    34921 aaatattttt ccttgtgata gatccggatt ttctcgcgcc catttaatta attcatctaa
    34981 tctcatttct tttttaactt tgattttcat tgttatatct cctcttgaac agtaaattta
    35041 tcgttaattg atacatatcc agtcacatta cataagatgc tatcaacatg aaaagtcaca
    35101 aaacagttgc gctcaacatc atttgaatag aatcttttat tacctgataa cttggggtta
    35161 tcccaagccc attggataag ttcaggtaaa ttcatttctt tttcaatttt gattttcatt
    35221 gtttccgccc ttttaaaata aagttagttg cttctgttcc tcatattcca aatcactttg
    35281 ctttatatat gtttcaagct cttccgctgt atcaaatgtc tttttcacac cttgccaacc
    35341 tggcacgata tgaccgtgaa agtaataagt gccatttact acatggatat gtgccactcg
    35401 ttcgttatcc tgatacagat atctcttaga gccgaaaaaa tgttttaagc gttctttacg
    35461 tgcgctatct gtcatggtcg tcactccctt taacaattag gcagtccaaa cgacatgcat
    35521 tcgtaatata gttcttcatt ccttatgctt gtcttatagt tctcaatcac attgctaact
    35581 tctttatgac tcattgcttt aacttgttcg tctgtatatt tttcgcagtc ttccaattcc
    35641 agttgttcct gtaatgacat cacatattca acttgttttt gagttgccat agttaaccct
    35701 cccacaagtc aaaagctctt tggacgtaaa acttcgcctt tgctaaatcc tcatgaccat
    35761 tctttaacgg tgctctagac aagtatttaa ttgcattacc tattgcgaat gctaattgtg
    35821 gtgggtactg tgccgtaact tgttcaataa aatctataat ttcaatgtcg ccgtatgtgt
    35881 aatgcgcagg ttgcttaacg ttgtcttgcg ttttgttcat atctactttt ctgttactga
    35941 ttatgctcat tatgcttcac tccatttctt gaacatttgg ttataagtga catcgaacca
    36001 gtacggatca cgtgaatgtt tttgaggcgt tccatcataa agccatggtc tcaatcttct
    36061 ctttctttct tcttcatatt ccgctctcac atttcgttgg tataggttca aaatcgcttt
    36121 ttttctgatt ttttctctct ctttttcttc atcttttatt tgactcttca tatattcaac
    36181 ttcatcttta gattttgagt cttttcttcc acacaataat tcatcgccgc gcattttatg
    36241 tttgtatctg tatctaagaa gttctggaga tatatgatat ttttctgaaa cttctctcaa
    36301 tgtcattagt tttcctttga tacgcactct tataactttt cttctagcca tcattccacc
    36361 tctaaatcta aaaccttgat atttataacg ttatatttta atagttcacc tggattatta
    36421 aataaatagt ccgccaaatt ctctttttct ttatcaatct gattgtaatt aacactttcg
    36481 acttctgtag gaattctaat gtcaacagaa gcattgatat aagcttgatg ttgcattcaa
    36541 tcacactcct aatccttcat ataaaacgga gaagtaaatc cgtcactatt caaattcaat
    36601 ccttttgccc aatcgacagg cttattcatg atagtttcga tttccttaag tccatttgaa
    36661 cctctaggta tttctacaat tacttcatca tggacatgtc caactatttt aaaacctgat
    36721 gcttcaagcc tagctataga aatcgcaagt aaatcccttg cagttgcttg aacaatattc
    36781 tcgactaact tcccaccata cgtttttaac tttgaccatt tacggttaag atctaagccc
    36841 ataaattcaa caacttgact accccaacta ttttcaccaa ctgaagcttt tggataagct
    36901 aaagctcttc cactaggcag ttcaatcatt agaaaacctt ttttcatata aaatctaagt
    36961 ccatgcgtat gatgcgtctt tcgggatttc acagtattaa ttgcagcctc ttggcaagcc
    37021 ttccaaaaat taactatgtt aggatttgcg ttacgccaac tatcaactaa accttgtaat
    37081 tcattttctt caatgcccat ttccaatgca cccatcgctt ttaaagctcc agcgccacct
    37141 tgataaccta aagctaattc ggacactttt cccttttgtc tgagagggtc gcctttagtt
    37201 atgctttcta ccgggacatt aaacatttga gaagccgatg cttcatatat ctttccgtgt
    37261 gtgttgaaca catctaaacg ccattgttct tttgcatacc atgctatgac tcttgcctct
    37321 attgcagaaa aatcacttac tgctagttca ttaccttctt cagcagtaaa tgtcgtccta
    37381 actaattgac ttaataagtc ttgaggatga acattgagta ataaatctaa atcgtcaaaa
    37441 cgttgttctt taataagatc tcttgctatt tctaattcag tatctgaaat ataatgcttt
    37501 gttaaattct gaagttgtac acctctacct gcccatcttc cagtaccggc accgtaaaat
    37561 tgaaacagac ctcttacccg ttcatcactg cacatcatgt catgcatttt gttgtatttt
    37621 ttcacactgg ttttagacat ttgcaatcta atttctagca tttttttagc ttttcctgtt
    37681 gcttctttta agtactcctg aaccgttttc ttttgtaaat taggtatatc taatccttgg
    37741 tcatccttta accaagccaa taattgtgta ggactattag gattttctaa acctgttata
    37801 tgtttagctt gattaagcaa ttcttcttta ctctgcttat cgagcacatt agctcctaac
    37861 atcaatgatt tagaaagctt aatacctctg tcgtttatat gttggtcaaa aacccaatat
    37921 gcttgttcaa ttgcagttac tggaaagtct ttaattttat gagcaatcgt catttctact
    37981 tctacatctc gaatacagta atctataaat tgttgccatt tttcaagatc atgttcaggc
    38041 aagtttcttg ttcttcctcc attaactttt gttggtttac aaggtataga gaaataacga
    38101 attaaatttt tacctgcttt atctttttgg ttttgtagtc ttaaaacttc tccaacttta
    38161 tcaagcgaag caggtaagcc aatacgcatt gaattaacca ttgtgcaaat ccattcttca
    38221 ggtggcatct gtttattaaa atgtttagca agacaagttc tttcgaaatt agcattgaat
    38281 gcatactttt ttacagcagg gtcaaataga gcaattttaa acgtctcata atcagcgtgg
    38341 aaaggctcat tatctacttt agtcatgtca atcgcactaa tcgctccacc atctatcgaa
    38401 taagctataa ttaaaatttc gaaatcttca gcttctgtgt atttataggc accacatttc
    38461 gaaatatcgt tactgctgta tgtttcaata tctatattca taaatttcaa attcttgaca
    38521 cctcaatttc tttaaaatta aagtggggct aaaaacccca cctattgact tataagaaat
    38581 cctcatcatc agtgtctaat tcatcaaaat catcttctgc tgcacttgca ccgccaagag
    38641 gttcgccttt ttctacaagt tgaatgttgt tcaatccaac tgcgataccc ttattaccat
    38701 ttgtgttgaa tggaaataaa ttgattgaag ctctaatata gtcaccactt acaatagttc
    38761 cagaatccgt taatctaatt ttgttttggt caataatacc aggtgcttgt ttgcttgatg
    38821 cgttaataaa ataagcgtct tgataattca catcatcttc tctttcagta tctccatcac
    38881 gtaatggaag tttcagattt gcaggaactt tgcctccaaa cttactaact tttccttctt
    38941 ctttagcagc ttctatagct tgttcaatgg cttttatcgt acttgtatct gatttaggaa
    39001 tgattaaact gattgaatac tttgcttctt gcccttcttg catactgtga ggttcaaaaa
    39061 tatgtgcata tgatgctctt acttttcctg taatcacttt agttttattt aatacttttg
    39121 ctttcatgtt tatataccgt cctttttaat ttttatagtt tgtcaaaatc atcttcagca
    39181 gattgcttta tagctggtcg tttatcagac tcggtagcaa gtgttaattt accttgtggc
    39241 ttttctataa agccttctgc aattttagaa aatgcttttt tgccgattaa tttttctaaa
    39301 ttcgtaatgc taagtaactt ggtttctgta atatcttcag gtttataacc cgcttcaact
    39361 aacttttcaa gcgttgcttt tgtatcagtt atcattcttc gcgaacgacc ttctacaagc
    39421 ttccaaccag gatagttttt atcatttcct ttcgcttgat ctagcgcata atgttctact
    39481 tcatcagccc attttttgat atcaggcagt ttatataaaa gttctgcaat ctcttcatca
    39541 cttaacaaat gtggtggctt ttgaggcaca ttttgcatgt attctgcacg tgttctacat
    39601 gaatgcttta tcttacagaa tctacaatga ctacctgctt taaactcacc ttcaccgtta
    39661 taagcaagtc tggctaatgg tttaacaaaa tcggttcccc attgaagtaa tcttgatatt
    39721 ggtaactctt cagtagaaaa gttatctatt cgtggttgta tgatagtcat gcgaactgta
    39781 tgaatgtcat acattaaact aagcagttca tatgcgccca agccatataa tctaagttga
    39841 ggattatcta tagctgaaac ttcaatgcct ttaccgtatt taaggtcaat aatttcaagt
    39901 acaccacctg aaaatataat gacatcacca gtaccaaaag attcagggac gtatttacct
    39961 aaatccaatt ttgtttcaaa taaagctatt acatcattat ccctactcaa agcttcgtta
    40021 tatttttctt ctacattagc tacatactct tccacatatt cacgcaattc ttcgctgtaa
    40081 tattgatttc tcttataatt ttgaaaagcc ttattaaact caaactgtgt taggccttca
    40141 tatttaaggc tgaaatataa ctcacttaac tcatgggcga atgtaccttc ttcggcaaaa
    40201 actgtacttt tatctgcaat accttcactt gccttaatac tcggtgggca gtttagccat
    40261 tgttttgctc cacttgcact aagctttgca tgagctctat ttgagtgatc tagcttcatg
    40321 catttattct cgcattcata aaatcaacaa ttttttcata atgctcttct ttgatagtag
    40381 atagcttatc cgcaccaagt tcgttaagtt tatttctaaa ttctttctta tcagaagtat
    40441 ctgctttttt aaggaactct tttcctactg ataaaatata atctttagtt aaatcagtag
    40501 acgtttcctt aacttcttca attgtttcca gttgagctgt ttcttctttt ggcattggtg
    40561 cttctttaac tttctcttgt acgattgatg aatccacagt tgatagttca gtatttaaca
    40621 cacgtaaatt cttatttaat agttttaatt cttcaaaaat atcttctaat attgccattg
    40681 attaaatcct ccttaaaatt ggttagctag acgaatcatt aacttgatac gatcttctat
    40741 ttctctaggg tcatcacttt gttcattcaa tcttgctaac aattcaaatt gctcttctaa
    40801 aatttctttt ttacgttcga cgacagttaa atgtaattgt gcttcgataa cacgccattt
    40861 tccccaactt tccatttcaa cctttccttt tttcttaagt ctcgaaagtg tggattttgc
    40921 atgtgttttc gatactccaa aaacttcaac tacatcatca ggattgaaat tgtcatatgt
    40981 tgcaaaatgt gatagtattt tttgttgtaa ggtcatatta ataactcctt atataattat
    41041 ttaagacaat tgctcatctt gcactgttac ttgctccaac aagtagcagt ttttttattc
    41101 tccataaaag tattctttat agaatatgaa tgttgcgata cttgcgaatc ccgcaatcga
    41161 ccacgctgta gtgaagtata gaaacggcat gagtacaatc gctaagaccg tgaagcacag
    41221 tactgctact aggtaacttt tatatgtgtc actcatttta ttctctcctt aaagtatttt
    41281 ctcttgcctt ttaattaaat acgcttctaa cttcggaata ttaattaact gtccagctgg
    41341 agaatagatg atacataagt tttttatacc taaatcatct tcgtgataat atttcaacca
    41401 gttgtatact gtacttctac ttactccgaa tagttgatga atttctgttg gttttgcgta
    41461 taactttttc acaaattttt cttcgcctct atatgtgttt tctggtgttg gtggtactat
    41521 gatttttggc atttctatca ctcctttcga taaatgttaa agtttgttat tattcgccct
    41581 gtattgaagt tctctatcta atgcatagaa aactttgttt atttctaagt agctgtaatc
    41641 acttttttta ataagctcta atatttccgc tcctaagtta cgttcctttt ccgttaaata
    41701 ggatgaagaa gcatcagctt tgctagaaac ttgtgggacg cctatacgca atccttctga
    41761 tcttgtgttc atttgtttat gctcctttcg tgtataatgt tgttatcaac ctaaggaggt
    41821 gataacatgc ccttgatatc tgatgaattt gatacactta ctaaagacca acaatatatc
    41881 ttgtccgtac tctacaaaga ttatttagaa tgcgtaaagt taggttcggt taaattaacc
    41941 tgcaataatt ttggaagtgc taaagatata catacaaagt attttcaaaa actacatttc
    42001 gaagatgtaa aatacgattt aaataaactt aaaaactctg ggttcctaaa cggcgtgtat
    42061 gctagtaaca ctatttatca tgtaacaatt tcagacaaga ctgttgttta ctttgaaaat
    42121 gagtttaaaa acaatttaaa aagtatcatt gatagcattt ctaaaattgc ttcaataatt
    42181 cctggtctct agttgggttt ataacttccc aatcatttgc catgaggtca tcggctgaag
    42241 gttgccaata tctgataagg tttgtcccat cgctatttga aatgatgcat tgtaaaaaac
    42301 tatcatttgt tggtaatatc ttagttcgat gactttcctt ccaatctttc cgtgtcatag
    42361 agacaagatt ttttgtagct atcttagttg cttcttgaat gttcatttgt tattcctcct
    42421 ttcgtgtata atgttgttat caacctaagg aggtgataag tatgaaagct tgtttatatc
    42481 tttctaatga taaatttgtt gaaatcgata atttagaaaa agtgataaag tcaggtcatc
    42541 gcggaactgt tgaaatatca aaagaaaaaa ttaaaagttc cttgttcact aatggctcat
    42601 atacttttgt tggagacaaa atagtagcta tcgcttcagc taaaatcgaa ttcatagaat
    42661 ttatcgatta atctctttaa gcaactctgc aactgctcgc aacagttcag ggttgtttct
    42721 tgtttctaaa ttactgtttg catgttttag taaattgagt tttaatttac ttttttcttt
    42781 agcgattcta aatttttgta acatttgttg ttcctccttt taagatgttt gtttaaattt
    42841 caaattggct aatatctaca ccgtatttaa tcgccatact cttaatcact gaaatgtata
    42901 tctcaaccaa tctaggttca tcagtaatca catctaattt tgacaacttg ttaatctggg
    42961 ttttcgttgc accattcgct agcattttgc ctttgcggtt ctgcatacga atttttaaat
    43021 tacagcgtcc tttttcttct aaagctttat atgcttcaga cttaactttc tggtgcattg
    43081 ctccgccacc taaatgttgt gcaatcgcag ataacatttt gtttgtgtcg ttacgccagt
    43141 ttttcgtttc aataccgaca atgtgacgaa tgcctgtgat ttcttgttgc atttgtttgt
    43201 taaactgttc ttggtctttt tgtgctttga acatcatctc taatgcttgc attggtgttt
    43261 gtggtacatt aagctgtgct tgttgtttaa tgtattcatc cattttatga aatgcatcaa
    43321 cataagttgc agtaaacaaa atgcctttac tacctgtcat cttgtttgct actatgtcgc
    43381 aacctttttt ggttagtagg taacaaggtt gtactttgtt ttgtgaatta acataggtgc
    43441 tttcttcaaa gaaattatga ctactcaatt ttgaggagtc ctctaaaacc ttgatataac
    43501 ctttaatgtc tcttactaaa ttgtcgtgtc gctttcctat catttccgca acttctctac
    43561 tgtctacata atgtgtttcg ttctgttcta ctatttgtaa tgcttgcatt tcagtttcct
    43621 ccttaagtta aaactttctt tttgcgtaag tcttcgttaa aaaaaatatc tcttccttct
    43681 tgaggtgtca attctaacgc aaaataaata ccatttatta ccgggtacga cggtttcgtt
    43741 ctcccgtgaa tcatattaga taaagtatct ctattaacac caatttcttc agaaagggtt
    43801 ttgatgttat gttctttcaa agccatttta gatttcaaaa gtttagcatc tataggcatt
    43861 tcttttcacc acctttcgta ttacgtaagt aatcttatca tgatgttacg aaagaggtca
    43921 agcactttac gaaagttttt tagaaaaata ttgcaaatgc cgaaagtttt ccttataata
    43981 gaactatcaa gtaaaaggag ctgtattacg atgtgctttt caaaaagaat gaaacaatca
    44041 agagaaaaac aaggtatgac tttggccgaa ctaggaagaa aaattggtaa aactgaagct
    44101 actgtacagc gttatgaaag cggaaatatc aaaaatttaa aaaacgatac tatagaaagt
    44161 atagctactg cattaaatgt taatcctgca tatttaatgg ggtgggttga agaaaacgat
    44221 gatgaagtac aacatcgtgc agctcacctt gaaggagaat tgacagatga tgaatggcaa
    44281 agagttttag attatgcaga ttatataaga agcaaacgta agtaaaggat gtatcagatg
    44341 ggattatatg aagaaacttt aatacaacat gattatattg aaataagaga ggctgatgtg
    44401 cttccagata atttggatgg ggtatggtta ggagatttaa ttttaataaa gcgtggctta
    44461 tcagatagag aaaaggcagg aattctcttt gaagaattag cacataataa acttacatac
    44521 ggtgatatag ccgattactc gaaattcaac aatcgcaagt tcgaaaatta cgcaaggcga
    44581 cacggcttta tctcagcagt cccgttacgc gaaattgtgg aagcttacaa ttatggtgta
    44641 cgtaacttgt atgagttgtc tgagtatctg caattgagtg aagaatacat attagaagca
    44701 atagaacaat ataaaaagat atatggtatt gggactcact acggcgaata ctcaattaca
    44761 tttgagccat tgagagtttt taaatataaa gaaatataaa caaaggagaa attaaaatga
    44821 aaagattatt aggtttaaca ttagcgagtg cgttagtttt aggcgcttgt ggtagccacg
    44881 acggcgataa gaaagaggaa agcaaaaaaa ctgaaacaaa gaaagataac aaagataaaa
    44941 agaaagaaac taaagaaaaa gcagaagcga aaaaagaaaa tgctaatcaa aacgataaca
    45001 ataatcaagt aaacaacgag aacaacacaa acgttaacaa cgatcaacaa accaatagac
    45061 ctttaactaa agacgaaata tcacaaagag taaaaaatgg tcacaatgtt aacggcatgg
    45121 tagatgcaga tggtaatact tggtaccaag cacaaggcgc aggtgacgtt ataggttaca
    45181 caaaacctga tggtacacaa tgcacagttg gtggttgtgt cacacctcag caacaagaac
    45241 aaataaacga agctaattat aaagagatgg aaaaatatgg gtattctcgt gaaaaatacg
    45301 atgcaattca aaaagaagct tctaaacttc aacaacaaaa agaaaatgga gaaataacag
    45361 cagaagaatt tactaatagg tatatagaat tatacgacta agtatcttac aatcaattaa
    45421 t
//
LOCUS       DAHHSC010000003        39693 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.003, whole genome shotgun sequence.
ACCESSION   DAHHSC010000003 DAHHSC010000000
VERSION     DAHHSC010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 39693)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39693)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39693
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.003"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            complement(86..1255)
                     /locus_tag="NBI57_000119"
     CDS             complement(86..1255)
                     /locus_tag="NBI57_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-glycerol glycerophosphotransferase family
                     protein"
                     /protein_id="HCD1821118.1"
                     /translation="MIKNTIKKLIEHSIYTTFKLLSKLPNKNLIYFESFHGKQYSDNP
                     KALYEYLTEHSDAQLIWGVKKGYEHIFQQYNVPYVTKFSMKWFLAMPRAKAWMINTRT
                     PDWLYKSPRTTYLQTWHGTPLKKIGLDISNVKMLGTNTQNYQDGFKKESQRWDYLVSP
                     NPYSTSIFQHAFHVSRDKILETGYPRNDKLSHKRNDTEYINGIKTRLNIPLDKKVIMY
                     APTWRDDEAIREGSYQFNVNFDIEALRQALDDDYVILLRMHYLVVTRIDEHDDFVKDV
                     SDYEDISDLYLISDALVTDYSSVMFDFGVLKRPQIFYAYDLDKYGDELRGFYMDYKKE
                     LPGPIVENQTALIDALKQIDETANEYIEARTVFYQKFCSLEDGQASQRICQTIFK"
     gene            complement(1683..3377)
                     /locus_tag="NBI57_000120"
     CDS             complement(1683..3377)
                     /locus_tag="NBI57_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000167965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-glycerol glycerophosphotransferase family
                     protein"
                     /protein_id="HCD1821119.1"
                     /translation="MTKTKQAIHIDNIYWERVQLYIEGHSEGVDLTSGQFVLRNLTET
                     KTLEANEMKIDGNTFICRFNVAILDDGYYLPMDKYLFVYHDQLEYIGQLNPNIIDQAY
                     AALNEEQIEEYNELTTQNGKVNYLLAYDAKVFRKGGVSQHTVYTITPEIASDVNEFVF
                     DIEITLPQEKSGVIATSAHWLHKQGHKASFESRSFLFKAIFNITKLLHIKRSKTILFT
                     SDSRPNLSGNFKYVYDELLRQKVDFDYDIKTVFKANITDRRKWRDKFRLPYLLGKADY
                     IFVDDFHPLIYTVRFRPSQEIIQVWHAVGAFKTVGFSRTGKKGGPFIDSLNHRSYTKA
                     YVSSETDIPFYAEAFGIREENVVPTGVPRTDVLFDEAYATQIKQEMEDELPIIKGKKV
                     ILFAPTFRGNGHGTAHYPFFKIDFERLARYCEKHNAVVLFKMHPFVKNRLNISREHRQ
                     YFIDVSDHREVNDILFVTDLLISDYSSLIYEYAVFKKPMIFYAFDLEDYITTRDFYEP
                     YESFVPGKIVQSFDALMDALDTEDYEVEKVVPFLDKHFKYQDGRSSERLVKDLFRR"
     gene            complement(3399..4424)
                     /locus_tag="NBI57_000121"
     CDS             complement(3399..4424)
                     /locus_tag="NBI57_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HCD1821120.1"
                     /translation="MINQVYQLVAPRQFDVTYNNVDIYGNHVIVRPLYLSICAADQRY
                     YTGRRDENVLRKKLPMSLVHEAVGEVVFDSKGVFEKGTKVVMVPNTPTEQHHIIAENY
                     LASSYFRSSGYDGFMQDYVVMAHDRIVPLPNDIDLSTISYTELVSVSYHAIQRFERKS
                     IPLKNSFGIWGDGNLGYITAILLRKLYPEAKIYVFGKTDYKLSHFSFVDDIFTVNQIP
                     DDLKIDHAFECVGGKGSQVALQQIVEHISPEGSIALLGVSELPVEVNTRLVLEKGLTL
                     IGSSRSGSKDFEQVVDLYRKYPDIVEKLALLKGHEINVCTMQDIVQAFEMDLSTSWGK
                     TVLKWTI"
     gene            complement(4417..5133)
                     /locus_tag="NBI57_000122"
     CDS             complement(4417..5133)
                     /locus_tag="NBI57_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HCD1821121.1"
                     /translation="MIYAGILAGGIGSRMGNVPLPKQFLDIDNKPILIHTIEKFILVS
                     EFNEIIIATPAQWISHTQDILKKYNITDQRVKVVAGGTDRNETIMNIIDHIRNVNGIN
                     NDDVIVTHDAVRPFLTQRIIKENIEVAAKYGAVDTVIEAIDTIVMSKDKQNIHSIPVR
                     NEMYQGQTPQSFNIKLLQDSYRALSSEQKEILSDACKIIVESGHAVKLVRGELYNIKV
                     TTPYDLKVANAIIQGDIADD"
     gene            complement(5661..6704)
                     /locus_tag="NBI57_000123"
     CDS             complement(5661..6704)
                     /locus_tag="NBI57_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HCD1821122.1"
                     /translation="MKALKLYGVEDLRYEDNEKPVIESANDVIVKVRATGICGSDTSR
                     YKKMGPYIKGMPFGHEFSGVVDAIGSDVTHVNVGDKVTGCPAIPCYQCEYCLKGEYAR
                     CEKLFVIGSYEPGSFAEYVKLPAQNVLKVPDNVDYIEAAMVEPSAVVAHGFYKSNIQP
                     GMTVAVMGCGSIGLLAIQWARIFGAAHIIAIDIDAHKLDIATSLGAHQTINSKEENLE
                     KFIENHYANQIDLAIESSGAKVTIGQILTLPKKGGEVVLLGIPYDDIEIDRVHFEKIL
                     RNELTVCGSWNCLSSNFPGKEWTATLHYMKTKDINVKPIISHFLPLEKGPETFDKLVN
                     KKERFDKVMFTIY"
     gene            complement(6728..6874)
                     /locus_tag="NBI57_000124"
     CDS             complement(6728..6874)
                     /locus_tag="NBI57_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1821123.1"
                     /translation="MVESMLTFMLGPLRQITDFYMEHLLVSNSIVIAGYFATGIFKKK
                     KVVN"
     gene            complement(6876..7931)
                     /locus_tag="NBI57_000125"
     CDS             complement(6876..7931)
                     /locus_tag="NBI57_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HCD1821124.1"
                     /translation="MKALVKTREGHGNLELLDKEVATPLDDKVKIKVHYAGICGTDIH
                     TYEGHYKVNFPVTLGHEFSGEIVEVGADVKDFKVGDRVTSETTFYVCNECEYCKSKDY
                     NLCNHRKGIGTQVDGAFTNYVIAREESLHHIPDEVSYQSAAMTEPLACAHHGVSKIQV
                     NSGDVAVVMGPGPIGLLVAQVLKSKGATVVVTGLDNDKVRLDKAEALHMDYVVNLQQT
                     DLKTYINGITDGYGADVVVECSGAVPAARQGLDIIRKKGFYSQIGIFKDAEIPFDMEK
                     VIQKEITVVGSRSQKPADWEPSLQLMADGLVNAEALVTKIYDISKWDEAYQHLKSGEG
                     IKALLKPLDLDENEGEN"
     gene            complement(7949..9208)
                     /locus_tag="NBI57_000126"
     CDS             complement(7949..9208)
                     /locus_tag="NBI57_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HCD1821125.1"
                     /translation="MSYFTDFVRGFLDLGATVILPVVIFLLGLFFRQKIGAAFRSGLT
                     IGVAFVGIFLVIDLLVKNLGPAAQAMVKNLGVSLNVIDVGWPATSSIAWASSVAAFII
                     PLGIIVNVVLLVTKVTKTMNVDIWNFWHYTFTAAMVYAVSGSIWQALLAAVIFQVICL
                     KVADWTAPMMSEFFDLPGVSIATGSTISYAPGIYLVKLLQKVPGLNKLDADPETIQKR
                     FGAFGESIFVGLILGLGIGVLAGYKPGDIINLGMSMAAVMVLMPRMVKILMEGLMPVS
                     ESARTWLNKRFGEREIYIGLDAAVALGHPAVISTALILVPITVLLAVILPGNQVLPFG
                     DLATIPFVVAFIVGAARGNIIHSVIVGTIMIAISLYIATDVAPIFTDMAKGTNVQMPK
                     GSSEISSIDQGGNIVNYLIFKLFSLFN"
     gene            complement(9435..9713)
                     /locus_tag="NBI57_000127"
     CDS             complement(9435..9713)
                     /locus_tag="NBI57_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCD1821126.1"
                     /translation="MKQVLVACGAGIATSTVVNNAIEEMAKEHNIKVDIKQIKITEVG
                     PYEDTADLLVTTAMTKKEYKFPVINARNFLTGIGIEETKQQILTELQK"
     gene            complement(9736..10203)
                     /locus_tag="NBI57_000128"
     CDS             complement(9736..10203)
                     /locus_tag="NBI57_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HCD1821127.1"
                     /translation="MGQQLVHKENIMLNLSATDKESVLSQMSDVLFQNGFVKSTFKDA
                     VIDREKEFATGLPTHLCSVAIPHTDVEHINHRTIGVAVLEKEVPFIEMGTLDQQTEVK
                     IVFMLAMDKVDDQLKLLQQLMQIFQSEEKLEQILRTKDETILATLINDYLEYN"
     gene            complement(10188..12284)
                     /locus_tag="NBI57_000129"
     CDS             complement(10188..12284)
                     /locus_tag="NBI57_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HCD1821128.1"
                     /translation="MNGDNQQILREIVLNPTIHGKELESIFGLSRRQLGYRIQKINLW
                     LEQEGYPKLERTSQGNFIVSSEIMTLFKRDVSEQQMLNGNNVIFSIETRRYYLMLMLF
                     SKENAMSLNHFSIDLQVSKNTVIHDINHVKEQLEDHGLSLKYSRKHGYEIVGDEFEVR
                     RFFIKLIDQRLNHDITKSEVLKALNLTFEDIAYQKDKIKQVEQFLKSRFIDKSLSSLP
                     YVLCVIRRRIQSGHVMNPLNINYQYLRDTKEYQATEIMTQHEPDLPEAEKLYLTLHLL
                     STSVQWTDLQESDNISNLTMAIAQMIHHFEQITFINIEDKEKLSQQLLLHLTPAFYRI
                     KYNLTDRDELINPLQGNYQSLFHMVKQSCQSLTEYFGKSLPDNEIAYLTMLFGGSLRR
                     QDENFDGKIKAIIVCTQGTSVSQMMLYELRNLFPEIIFLDAISLRTFENYTLDYDIVF
                     SPMFVLTHKKLFITKVALSENEQRKLRKEVMKYINKESADIDKEINKLMALIERTTTV
                     NDITELRDGLEDFIANYNSISTINGSIVTQNKTLDLADLIPARHVKRMHHVENIDEAI
                     AKASDVLVANHFIDIKYIHEMQQVFDDSYMVIMQNIAIPHAYSEKHVHKTAMSMLILQ
                     EPIYMSDGTAIHIIVPIAAVDKVTHLRALLQLRDVAQDNDAIKRIIQSRKNSDVNEIL
                     KNYSNKEARENGWDSN"
     gene            complement(12415..12512)
                     /locus_tag="NBI57_000130"
                     /pseudo
     CDS             complement(12415..12512)
                     /locus_tag="NBI57_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498807.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(12622..13557)
                     /locus_tag="NBI57_000131"
     CDS             complement(12622..13557)
                     /locus_tag="NBI57_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HCD1821129.1"
                     /translation="MKRKIIMDCDPGHDDAIALILAGAIDSPLEILAVTTVAGNQSVD
                     KNTTNALNVLDIMGRQDIAVAKGADRPLIKPAAFASEIHGESGLDGPKLPSTPSRQAV
                     AMPASDVIINKVMTSDTPVTIVATGPLTNVATALIREPRIAEHIESITLMGGGTFGNW
                     TPTAEFNIWVDAEAAKRVFESGITINVFGLDVTHQVLADNHVIERFESINNPVAQFVV
                     ELLQFFKKTYKTHFNMDGGPIHDACTILYLLQPELFTMVPVNIDIEHQSPLTYGTMAV
                     DLNHVTGKPANAYFATAVDVEEVWNLIDHKLRTYE"
     gene            13919..15448
                     /locus_tag="NBI57_000132"
     CDS             13919..15448
                     /locus_tag="NBI57_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HCD1821130.1"
                     /translation="MKSLFEKAQQFGKSFMLPIAILPAAGLLLGIGGALSNPNTVKAY
                     PILDITLLQNIFTLMSAAGSIVFQNLPVIFAIGVAIGLSRSDKGTAGLAALLGFLIMN
                     ATMNGLLTITGTLAKDQLAQNGQGMVLGIQTVETGVFGGIITGIMTAILHNKYHKVVL
                     PPYLGFFGGSRFVPIVTAFAAIFLGVLMFFIWPSIQAGIYHVGGFVTKTGAIGTFVYG
                     FILRLLGPLGLHHIFYLPFWQTALGGTLEVKGHLVQGTQNIFFAQLGDPDVTKYYSGV
                     SRFMSGRFITMMFGLCGAALAIYHTAKPEHKKVVGGLMLSAALTSFLTGITEPLEFSF
                     LFVAPILYVIHAFFDGLAFMMADIFNITIGQTFSGGFIDFLLFGVLQGNSKTNYLYVI
                     PIGIVWFCLYYIVFRFLITKFNFKTPGREDKAAAQQVEATERAQTIVAGLGGKDNIEI
                     VDCCATRLRVTLNQNDKVDKVLLESTGAKGVIQQGTGVQVIYGPHVTVIKNEIEELLG
                     D"
     gene            complement(15768..16721)
                     /locus_tag="NBI57_000133"
     CDS             complement(15768..16721)
                     /locus_tag="NBI57_000133"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017804598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HCD1821131.1"
                     /translation="MNKFKGNKVVLIGNGAVGSSYAFSLVNQSIVDELVIIDLDTEKV
                     RGDVMDLKHATPYSPTTVRVKAGEYSDCHDADLVVICAGAAQKPGETRLDLVSKNLKI
                     FKSIVGEVMASKFDGIFLVATNPVDILAYATWKFSGLPKERVIGSGTILDSARFRLLL
                     SEAFDVAPRSVDAQIIGEHGDTELPVWSHANIAGQPLKTLLEQRPEGKAQIEQIFVQT
                     RDAAYDIIQAKGATYYGVAMGLARITEAIFRNEDAVLTVSALLEGEYDEEDVYIGVPA
                     VINRNGIRNVVEIPLNDEEQSKFAHSAKTLKDIMAEAEELK"
     gene            complement(17032..17139)
                     /locus_tag="NBI57_000134"
     CDS             complement(17032..17139)
                     /locus_tag="NBI57_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1821132.1"
                     /translation="MSVCTPTIARIYLTNSKNIVVFLEKSKHDNKNVKV"
     gene            17294..18439
                     /locus_tag="NBI57_000135"
     CDS             17294..18439
                     /locus_tag="NBI57_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide dioxygenase"
                     /protein_id="HCD1821133.1"
                     /translation="MLTEQEKDIIKQTVPLLKEKGTEITSIFYPKMFKAHPELLNMFN
                     QTNQKRGMQSSALAQAVMAAAVNIDNLSVIKPVIMPVAYKHCALQVYAEHYPIVGENL
                     LKAIQDVTGLEEHDPVIQAWAKAYGVIADVFIQIEKEIYDQMMWIGFKPFKITNIKQE
                     SEDIKSFTVETEEYDFSEFTPGQYITVDVSSDKLPYRAKRHYSIVSGEKNHLTFGVKR
                     DVTTEHEGEVSTILHDEIKEGDMINLAAPVGGFVLENTTEPQLFLGSGIGVTPLVAMY
                     EAASAKGLDTQMVQVAENEQHLPFKDNFNSIASHYDNAKLYTHLKDKQGYIGTEELQA
                     FLANKPEIYICGGTKFLQSMIEALKSLNYDMDRVHYETFIPRLSVAV"
     gene            18465..18638
                     /locus_tag="NBI57_000136"
     CDS             18465..18638
                     /locus_tag="NBI57_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1821134.1"
                     /translation="MKLNQRYVKVFALYFVSIVTANIIVKNNNLIKTLIQTIAGYTVF
                     AVGLKYLTKRKNK"
     gene            18795..19151
                     /locus_tag="NBI57_000137"
     CDS             18795..19151
                     /locus_tag="NBI57_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HCD1821135.1"
                     /translation="MTVDIGRIYDNKDNTDAIRILVDRVWPRGISKRTANLDYWLKDI
                     APSTELRQWFQHDPKLFGAFKEKYEKELRDQDAQKDAFEKLKDIVNQHNHVLLLYAAK
                     DTKHNQAVVLQQLLNT"
     gene            19349..20824
                     /locus_tag="NBI57_000138"
     CDS             19349..20824
                     /locus_tag="NBI57_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000669654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCD1821136.1"
                     /translation="MKFKRLATIFSAVLVLSGCGSMHSSGKDLNISLPLKTKSIAPYE
                     TDVPVKIGAAESLFKTNDQGKIEKALVKSYHQPNDTTLDIELKDNIKFQNGQKLTAEK
                     VKSSLENSMKKSDLVKYSLPISSITAKGQKLTIKTNSAYPELVSELANPFMAIYYTDA
                     KSDVNQTPVGTGPYQIKDYKQSRKISLSNFKDYWQGKPKLDHITVTYQEDGNNRVRNL
                     ESQKDDLITDVPVNKVHDIENNQNLKVSKESGFRTSLLMYNHTNKKMTKSVREALDHI
                     IDRQGIADHIYQGYAKPATSPFNDKIPYIKEPKLTKQNIEQAKTLLAKDGYTKEHPLK
                     IKLITYDGRPELSKIAQVLQSDAKKANIEIDIKSVDDIEGYLKDRSAWDATMYSFGTI
                     PRGDTGYFFNQAYKKDGAINKGDYNNSNVDDLINQLNHTVDVKERHNISNDIIKLSSR
                     DVPNSYIAYNDQIVAANSKVKNYKVTPEGIYLIDYRTTIER"
     gene            complement(21138..22280)
                     /locus_tag="NBI57_000139"
     CDS             complement(21138..22280)
                     /locus_tag="NBI57_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family intramembrane metalloprotease"
                     /protein_id="HCD1821137.1"
                     /translation="MSSASTQSTKTSDIHNESINKQMEAKAHETAQNADLKTEARSLF
                     DNATKSIGRLAGNDESLNLNLKDMFSEVFKPHTKNEADEIFIAGTAKTTPAICDISEE
                     WGKPWLFSRVFIAFTVTFIGLWVMAAIFNNTNAIPGLIFIGALTVPLSGLFFFYESNA
                     FKNISIFEVIIMFFIGGVFSLLSTMVLYRFVVFSDQFERFGSLTFFDAFLVGLVEETG
                     KALIIVYFVNKLKTNKILNGLLIGAAIGAGFAVFESAGYILNFALGENVPLLDIVFTR
                     AWTAIGGHLVWSAIVGAAIVIAKEQHGFEFKDIFDKRFLIFFLSAVGLHGIWDTSLTV
                     LGSDTLKIFILIVIVWILVFILMGAGLKQVNLLQKEFKEQQKKVDE"
     gene            22721..24298
                     /locus_tag="NBI57_000140"
     CDS             22721..24298
                     /locus_tag="NBI57_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl CoA:acetate/3-ketoacid CoA transferase"
                     /protein_id="HCD1821138.1"
                     /translation="MEVTCLKQITWHDLQHIIKDGDVIGLPALAVANLPAEVLRAVLA
                     QHDTYHTPKDLTFILANDIHSLGAAPDLDDFIERRMIKRVIMSILTASSKTAQAMKNN
                     DIEAYFLPQGIIATHYRQSNQLLPGVITKIGLNTAVDPRYGGGKVNTRTTDDLVSLVT
                     INDETYLHYTFPSVDVALLRGTYADQQGNIYLTQEAYLSECYHVALNAKANHGKVIVQ
                     VKALVDDYQLKPNEVVIPGNLVDYVYVTEDEKNHRQVIQSHYLPALSGEERIDGIPEP
                     ALPFNSRKLILRRAAQFLTYGDTISIGYGINNELSNLLHEECVEHDVQPILDVGIFGG
                     FVGSREHFGMNYNADVRMPHDRAWDFIYNNGVSVAYLSFAEVDQYGNVNVSYFNDRLN
                     GCGGFIDITQSVNKIIFSGTFVAGSHVSCHNQRLNIETEGQNQKFVSDVSHIDFNAQY
                     SQLLEQEVYFVTDRAVFELTNQGLKLIEIAPGLDLHKDILNQMAFKPIIADHLKLIDT
                     SIYKEKWGQLKQSIHKV"
     gene            24324..25829
                     /locus_tag="NBI57_000141"
     CDS             24324..25829
                     /locus_tag="NBI57_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl--CoA ligase"
                     /protein_id="HCD1821139.1"
                     /translation="MNFDWIKTRSDFDDDKPAVIDHAKQTSWTYQQLNARADNMAHYL
                     TSQGVKKGDVIGIFAPNDIAILDLLFACFKTGAVFLPLNWRLNPKEIAAIVEDAQLKL
                     LFYAEKHLSSLTDIDQNLLHMDIDVAQYDEIVNPDYHQPFQATPVEPQDLAALIYTSG
                     TTGSPKGVMFSYESFVHNGANLELTYKFNSNYITIVSTPMFHVLGFNDTVLPVLMSGG
                     TLILQRYFNGEELNDMIAQYHPTFIIMIPTMYYSTLRASNFNPENFRAMDYIIQGGSQ
                     PLPSIQAAFKQYGINIINGYGLTEAPLVLVNTPENSKRKPMSIGKAVMFVDARILDDN
                     GEEVPTGEIGELAIKAKNVTPGYWNKPAETAKAFHGRYLLTGDLAKMDDDGDIFIIDR
                     KKELIITGGENVLPSEVENALAEHPLVDRCVVVGYDHPKYGESIAAAIILREDEPHYA
                     EILDQHMRSRLAGYKVPRMYVPVTHMPLNSTQKPDKLAIRQMMNDKVSQTL"
     gene            25941..27152
                     /locus_tag="NBI57_000142"
     CDS             25941..27152
                     /locus_tag="NBI57_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HCD1821140.1"
                     /translation="MTFEKETVLKTLFPEDVLSIAKGLTDGEVEFLQQVDSLLESKYR
                     ENINQHWIDATVPEDYFKDLGELNYFNNPLLYKDRPNAKMPSQLFQFFMSYLLARFDI
                     SLATLLGVHQGLGHNTFYFGGSKEQIAKYVPKLQSHELRTCFALTEPEHGSDVAGGLE
                     TVAERQGDTWVINGEKKWIGGAHVSDVIPVFAVNKKTGKPHCFVVRPEQDGVDIEVID
                     NKIALRIVPNALIKLTNVKVDEADRLQNITSFKDIAKILYSTRAGVAYMATGGMAGAL
                     RATLDYVTERKQFGKPISKYQLIQEKLAMMQGNLAQAMATCAQLANMQAHGEYDEVAT
                     STAKMMNALRLRETVAMGRGITGGNGILADDYDIARFFSDAEAIYTYEGTHEINALVI
                     GRALTGDSAFV"
     gene            27338..29599
                     /locus_tag="NBI57_000143"
     CDS             27338..29599
                     /locus_tag="NBI57_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase/enoyl-CoA
                     hydratase family protein"
                     /protein_id="HCD1821141.1"
                     /translation="MTINKVTVLGAGTMGAQLAALFVNAGLKVKLLDIVVDKNDPNLI
                     AKKSYDKITDKKRPLLFDLNLVSHLTYGNFDDDLVNDDADLYIEAVKEDIEIKHAVWQ
                     QVLQHAKEDALFATNTSGIPINAIAQAFNEKDQERFFGLHFFNPPRIMKLVELIPTSH
                     TKESIILDVKNFAQNVLGKGVIVVNDVPGFVANRVGTQTMNDIMYRAEQHKLSIVDVD
                     ALTGQAIGRPKTGTYALSDLVGLDIAVSVIKGMQQVPEETPYFHDVKIVNTLFDNGAL
                     GRKTKQGFYKKDKETKARLVYDVEKQDYVPVSQPQLPILNEFNKDLVHNLDVIFNAQD
                     EAGLFLWETLRNNFYYSAINVPKATDDFRDIDRALVWGFNWKLGPFQLWDAMGYERVK
                     TRMEDELGDLPQWISDLDGGFYKQDETIEYATPVSHFVKDELWDKGDAKLSVTHDDQL
                     LLKLQSKNNVITDEFNDALVDAIDLLENDHYTSMVIYADGNNFSVGANLFLMKKAHED
                     GLVDDVVAQSIDKLHYSFNRLKYSLKPVVTAVQGRALGGGCELVLYSPIVVAASETYI
                     GLVEAGVGLLPSGGGLAEMADRILRTSHKFDDKQASMTKVLTNIAFAKVSTNAFEARR
                     YGYLRDTDTIIFNTAQRVEVALKRAKYEAETNYIPNPRHQYIALGEDFKALIQGQLDA
                     QRRGHFISDHDYHIALNIATILAGGDLPRNTFINQRYIQSLEKIGFIDLLKSKKSYER
                     IAHILKTGKPLRN"
     gene            29629..30813
                     /locus_tag="NBI57_000144"
     CDS             29629..30813
                     /locus_tag="NBI57_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HCD1821142.1"
                     /translation="MQEAYIVAYGRSAAAKAKQGALFHERPDDVAAKVLQGVLKRIDG
                     KFNKNMIEDVIVGTAFPEGLQGQNIARTIALRAGLSDTVPGQTVNRYCSSGLQTIAIA
                     ANQIMAGQGDILVAGGVELMSAVPMGGNEPTNNPTLQYDDIGASYPMGLTAENVASQF
                     DVSREDQDAYAVRSHQRAYDAQRDGRFKDEIIPIRVNSVEYTNAGPKVHTNIFDQDEF
                     IRPDTTMEALAKLRTVFKADGTVTAGTSAPLSDGAGFVVLMSGDKVKELGVTPIARFV
                     GYKAVGVDPKIMGIGPAYAIPEVLSLSNLSVEDIDLIELNEAFASQTIASIKEVGLDI
                     SRTNVNGGAIALGHPLGATGAMLTARLLNEMGRRPDSRYGMVTMCIGVGMGAAAIFEY
                     VR"
     gene            31111..31218
                     /locus_tag="NBI57_000145"
     CDS             31111..31218
                     /locus_tag="NBI57_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1821143.1"
                     /translation="MCVRTRLVSSSSVRLSKAIIIAVIVVYHLDVRGLF"
     gene            complement(31400..33376)
                     /gene="coa"
                     /locus_tag="NBI57_000146"
     CDS             complement(31400..33376)
                     /gene="coa"
                     /locus_tag="NBI57_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphylocoagulase"
                     /protein_id="HCD1821144.1"
                     /translation="MKKQIISLGALAVASSLFTWDNKADAIVTKDYSKESRVNEKSKK
                     GATVSDYYYWKIIDSLEAQFTGAIDLLEDYKYGDPIYKEAKDRLMTRVLGEDQYLLKK
                     KIDEYELYKKWYKSSNKNTNMLTFHKYNLYNLTMNEYNDIFNSLKDAVYQFNKEVKEI
                     EHKNVDLKQFDKDGEDKATKEVYDLVSEIDTLVVTYYADKDYGEHAKELRAKLDLILG
                     DTDNPHKITNERIKKEMIDDLNSIIDDFFMETKQNRPNSITKYDPTKHNFKEKSENKP
                     NFDKLVEETKKAVKEADESWKNKTVKKYEETVTKSPVVKEEKKVEEPQLPKVGNQQEV
                     KTTAGKAEETTQPVAQPLVKIPQETIYGETVKGPEYPTMENKTLQGEIVQGPDFLTME
                     QNRPSLSDNYTQPTTPNPILEGLEGSSSKLEIKPQGTESTLKGIQGESSDIEVKPQAT
                     ETTEASQYGPRPQFNKTPKYVKYRDAGTGIREYNDGTFGYEARPRFNKPSETNAYNVT
                     TNQDGTVSYGARPTQNKPSETNAYNVTTHANGQVSYGARPTQKKPSKTNAYNVTTHAN
                     GQVSYGARPTQKKPSKTNAYNVTTHANGQVSYGARPTYKKPSETNAYNVTTHANGQVS
                     YGARPTQKKPSETNAYNVTTHADGTATYGPRVTK"
     gene            33566..33910
                     /locus_tag="NBI57_000147"
     CDS             33566..33910
                     /locus_tag="NBI57_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="complement inhibitor SCIN family protein"
                     /protein_id="HCD1821145.1"
                     /translation="MTTQMKIKTYLVAGIKAALLDTTGIKLASKSETTSHTYQHQALV
                     DQLHELIANTDLNKLSYLNLDAFQKRDILAAHYIAKSAIRTKNLDQMTKAKQRLESIY
                     NSISNPLHSQNN"
     gene            complement(34074..35837)
                     /locus_tag="NBI57_000148"
     CDS             complement(34074..35837)
                     /locus_tag="NBI57_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphoryl diester phosphodiesterase
                     membrane domain-containing protein"
                     /protein_id="HCD1821146.1"
                     /translation="MKRISKDIWAVFKLLYQNKGRFSINALLLQLIMIFISSTYLILL
                     FNMMLKVAGQSQLTINNWMEIISHPASVILLIIFILSVAFLIYVEFSLLVYMVYAGFD
                     RQIITFKSIFKNAFVNVRKLIGVPVIFFVIYLMLMIPIANLGLSSVLTKNIYIPKFLT
                     EELMKTTKGIIIYGTFMIAVFILNFKLIFTLPLTILNRQSLFKNMRLSWQITKRNKFR
                     LVIEIVILELIIGAILTLIISGATYLAICVDEEGDKFLVSSILFVVLKSALFFYYLFT
                     KLSLISVLVLHLKQENVLDQPGLEFKYPKPKRKSRFFIISMVLAVTCFIGYNMYLLYN
                     NTINTNISIIGHRGFEDKGVENSIPSLKAAAKANVEYVELDTIMTKDKQFVVSHDNNL
                     KRLTGVNKNISESNFKDVVGLKMRQNGHEAKLVSLDEFIETAKQSNVKLLVELKPHGK
                     EPADYTQRVIDILKKHGVEHQYRVMSLDYDVMTKLKKEAPYLKCGYIIPLQFGHFKET
                     SLDFFVIEDFSYSPRLVNQAHLENKEVYTWTINGEEDLTKYLQTNVDGIITDDPALAD
                     QIKEEKKDETYFDRSIRILFE"
     gene            complement(36159..36914)
                     /gene="pflA"
                     /locus_tag="NBI57_000149"
     CDS             complement(36159..36914)
                     /gene="pflA"
                     /locus_tag="NBI57_000149"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase-activating protein"
                     /protein_id="HCD1821147.1"
                     /translation="MLKGHLHSVESLGTVDGPGLRYILFTQGCLLRCLYCHNPDTWKI
                     SEPSREVTVDEMVNEILPYKPYFDASGGGVTVSGGEPLLQMPFLEKLFAELKENGVHT
                     CLDTSAGCANDTKAFQRHFEELQKHTDLILLDIKHIDNDKHIRLTGKPNTHILNFARK
                     LSDMKQPVWIRHVLVPGYSDDKDDLIKLGEFINSLDNVEKFEILPYHQLGVHKWKTLG
                     IAYELEDVEAPDDEAVKAAYRYVNFKGKIPVEL"
     gene            complement(36937..39186)
                     /gene="pflB"
                     /locus_tag="NBI57_000150"
     CDS             complement(36937..39186)
                     /gene="pflB"
                     /locus_tag="NBI57_000150"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HCD1821148.1"
                     /translation="MLETNKNHATAWQGFKNGRWNRHVDVREFIQLNYTLYEGNDSFL
                     AGPTEATSKLWEQVMQLSKEERERGGMWDMDTKVASTITSHDAGYLDKDLETIVGVQT
                     EKPFKRSMQPFGGIRMAKAACEAYGYELDEETEKIFTDYRKTHNQGVFDAYSREMLNC
                     RKAGVITGLPDAYGRGRIIGDYRRVALYGVDFLMEEKMHDFNTMSTEMSEDVIRLREE
                     LSEQYRALKELKELGQKYGFDLSRPAENFKEAVQWLYLAYLAAIKEQNGAAMSLGRTS
                     TFLDIYAERDLKAGVITESEVQEIIDHFIMKLRIVKFARTPDYNELFSGDPTWVTESI
                     GGVGIDGRPLVTKNSFRFLHSLDNLGPAPEPNLTVLWSVRLPDNFKTYCAKMSIKTSS
                     IQYENDDIMRESYGDDYGIACCVSAMTIGKQMQFFGARANLAKTLLYAINGGKDEKSG
                     AQVGPNFEGINSEVLEYDEVFKKFDQMMDWLAGVYINSLNVIHYMHDKYSYERIEMAL
                     HDTEIVRTMATGIAGLSVAADSLSAIKYAQVKPIRNEEGLVVDFEIEGDFPKYGNNDD
                     RVDDIAVDLVERFMTKLRSHKTYRDSEHTMSVLTITSNVVYGKKTGNTPDGRKAGEPF
                     APGANPMHGRDQKGALSSLSSVAKIPYDCCKDGISNTFSIVPKSLGKEPEDQNRNLTS
                     MLDGYAMQCGHHLNINVFNRETLIDAMEHPEEYPQLTIRVSGYAVNFIKLTREQQLDV
                     ISRTFHESM"
BASE COUNT        12540 a         7294 c         6456 g        13403 t
ORIGIN      
        1 aaataataac atacaagcaa acatttcgtt tattatttat atcacttaac taattaattt
       61 ataatttttt attgttttta agttatcact taaaaatcgt ttggcaaatt cgttgtgacg
      121 cttgtccatc ttctaatgaa cagaattttt gataaaatac cgttcgtgct tcaatatact
      181 catttgcagt ctcatcgatt tgttttaatg catcaatgag tgctgtttga ttttcaacaa
      241 ttggacctgg caactctttt ttataatcca tgtaaaaacc tctaagctca tcgccatatt
      301 tatctaagtc atatgcatag aaaatttgcg gacgctttaa tacaccgaag tcgaacatga
      361 cagatgagta gtcggtaact aacgcatcgc tgattaagta taaatccgaa atgtcttcat
      421 aatctgaaac gtctttcaca aaatcatcat gttcatcaat acgtgtcaca actaaataat
      481 gcatgcgtaa taaaataaca taatcatcat ccagcgcttg acgcaaagct tctatatcaa
      541 agttaacatt aaattgatat gaaccttctc gaatcgcttc atcgtcacgc caagttggcg
      601 cgtacataat cactttttta tctaatggaa tatttaatct tgtcttaata ccattaatat
      661 attcagtatc attgcgttta tgtgataatt tatcatttct tggataacct gtttccaaaa
      721 tcttatctcg actaacatga aatgcatgtt gaaatatcga tgtcgaatat ggattaggtg
      781 acactagata atcccaccgt tggctttctt ttttaaagcc atcttggtaa ttttgagtat
      841 ttgttcctag cattttaacg ttactaatat ccaaaccaat cttttttaat ggcgtgccat
      901 gccatgtttg taagtacgtc gttcgcggtg atttatataa ccaatctggt gtacgtgtgt
      961 taatcatcca cgctttcgct cttggcatcg ctaaaaacca tttcattgaa aactttgtaa
     1021 catatggtac attgtactgt tggaatatgt gttcatatcc ttttttcaca ccccatatta
     1081 attgggcatc gctatgttca gttaagtatt catataatgc tttggggttg tcgctgtatt
     1141 gtttaccatg aaagctttca aaataaatta gattcttgtt tggcaatttt gatagtaatt
     1201 taaaagtcgt atatatacta tgttctatca attttttaat tgtattttta atcatgtcgt
     1261 acctccgacg tgtttttgta attatattaa tatgtatgag caagctcatt gtaaccatgc
     1321 ctattatagc atttcatcat aaaatacatt taaccattac acttgtcgtt aattatcata
     1381 cgaaatacat gattaatgta ccactttaac ataacaaaaa atcgttatcc attcataacg
     1441 tatgtgttta cacatttatg aattagataa cgattggatc gattatttta ttttacaaaa
     1501 tgacaattca gttggaaggt gattgctttt gattgaatcg ccttatgcat gaaaaatcaa
     1561 aaggttattc tcattgtata gtcctgcttc tcatcatgac atgttgctca cttcattgtc
     1621 agaacccttc ttgaaaacta tgccttatga ctcatttgca tggcaagtaa tatatgccaa
     1681 cattagcgtc taaacaaatc tttgactaaa cgttcacttg agcgaccatc ttgatattta
     1741 aaatgtttat ctaagaatgg tacaactttt tcaacctcat aatcttcagt gtccaaagca
     1801 tccattaatg catcaaagga ttgtacaatt ttacctggaa caaatgattc atatggttca
     1861 tagaaatcac gcgtcgtaat gtaatcttct aagtcaaatg catagaaaat catcggcttt
     1921 ttaaatactg catattcata tattaaagat gaataatcac taatcaacaa gtctgtaaca
     1981 aagagaatat cgttaacttc acgatgatct gacacatcga taaagtattg tctatgttca
     2041 cgtgaaatat taagtctatt ttttacgaac ggatgcattt tgaataacac aactgcatta
     2101 tgcttctcgc agtatcttgc taaacgttca aaatcaattt taaaaaatgg ataatgtgcc
     2161 gtaccgtgac cattacctct aaatgtcggt gcgaatagaa taactttctt accttttata
     2221 attggcaatt catcttccat ctcttgttta atttgtgttg cataagcttc atcaaatagt
     2281 acatcagtac gtggtacacc tgttggtaca acattttctt ctctaattcc aaatgcttca
     2341 gcataaaatg gaatatcggt ttctgatgaa acatatgctt tcgtgtaact acgatggttt
     2401 aatgaatcga taaacggacc acctttttta cctgtacgac taaagccaac tgttttaaag
     2461 gcaccaacag catgccacac ttgaataatt tcttgtgatg gtctaaagcg aaccgtataa
     2521 attaatggat ggaaatcatc aacaaaaata taatcggcct taccaagtaa atatggcaat
     2581 ctaaacttgt ctctccattt gcgtctatcc gtaatattcg ccttaaatac cgttttaata
     2641 tcataatcaa aatctacttt ttggcgtagt aactcatcat atacatactt gaaattccct
     2701 gataaattcg gacgcgaatc tgatgtgaat aatattgttt tgcttctttt aatatgtagt
     2761 aactttgtaa tattaaaaat agctttaaat aagaaacttc tactttcaaa tgaagcttta
     2821 tgaccttgtt tatgaagcca gtgtgcactt gtcgcaatga cccctgattt ctcttgaggt
     2881 aaggtgattt caatatcaaa tacaaattcg ttaacgtcac ttgctatttc cggagtaatg
     2941 gtatagaccg tatgttgtga tacgccacct ttacggaaaa ctttagcatc atacgctaat
     3001 aaatagttca cttttccatt ttgtgtagtc agctcattgt attcttcaat ttgctcttca
     3061 tttaatgccg cataagcttg atcaataata tttggattaa gttgtccaat atactctaac
     3121 tggtcatgat aaacaaataa atatttatcc attggtaaat aatacccatc gtctaatatt
     3181 gcgacgttga atctacatat aaatgtatta ccgtctattt tcatttcatt tgcttctaat
     3241 gtttttgttt cggttaaatt cctcagaaca aattgtcctg atgttaaatc gacaccttca
     3301 ctatgtcctt caatatataa ctgaacacgt tcccagtata tgttatcaat atgtattgct
     3361 tgtttcgttt ttgtcattgt tattccccca tcggtttatt aaatcgtcca tttcaatact
     3421 gtttttcccc aagatgtcga taaatccatt tcaaacgctt ggacgatatc ttgcatcgta
     3481 catacattaa tttcatgtcc ttttaataat gctaactttt caactatgtc tgggtactta
     3541 cgatataaat caacaacttg ctcaaaatct ttagagccgc ttcgactact accaatcaac
     3601 gttaatcctt tttcaagtac taatcgtgta ttcacttcca cgggtaattc acttacgcct
     3661 aacaaagcaa tactgccttc tggtgaaata tgttcaacta tttgttgaag tgcaacttga
     3721 cttcctttac ctccaacaca ttcaaatgca tgatcaattt taagatcatc tggtatttga
     3781 tttactgtaa agatgtcatc tacaaatgaa aaatgactta atttatagtc tgtcttacca
     3841 aatacataaa ttttagcttc tgggtacaac ttacgtagca aaatagcagt aatataacct
     3901 aagttaccat caccccaaat accaaagctg tttttcaaag gtatagattt acgttcaaat
     3961 cgttgtatag catgataact tactgacact aactctgtgt atgaaatcgt actcaaatca
     4021 atgtcattag gcagcggaac gatacgatca tgtgccatca caacgtagtc ttgcataaaa
     4081 ccatcataac cactagatct aaaataacta gaggctaagt aattctccgc gataatatga
     4141 tgttgctctg taggtgtatt cggcaccatt actactttcg tacctttttc aaatactcct
     4201 ttactatcga atacaacttc accaacagct tcatgaacta atgacattgg caattttttg
     4261 cgcagtacat tttcatctct tcgacctgtg taataccttt gatcagctgc acaaatagac
     4321 aagtataaag gtcttacgat gacatgatta ccataaatat caacattatt atatgtgacg
     4381 tcgaactgtc tcggtgcaac gagttgatat acttgattaa tcatcggcaa tatcaccttg
     4441 aataatggca tttgctactt ttaaatcata cggtgttgtc actttaatgt tgtatagttc
     4501 tccacgtacc aatttaactg catgtccaga ttcgacaatt attttacatg catctgataa
     4561 gatttctttt tgttcactac ttaaggcgcg ataactatct tgtaataatt taatattaaa
     4621 tgattgtggt gtttggcctt gatacatttc attccttaca gggatactgt gtatgttctg
     4681 tttatcttta gacattacaa tcgtatcaat tgcttcaatg actgtatcta ctgcaccata
     4741 ttttgctgct acttcaatgt tctctttaat aatacgttga gttaaaaatg gtcttacggc
     4801 atcatgagtt acaatcacat catcattatt aattccattt acattgcgaa tatggtcgat
     4861 aatgttcata attgtttcgt ttcgatccgt accacctgca actactttga cacgttgatc
     4921 tgtaatgtta tattttttta aaatatcctg tgtatgggaa atccactgtg ctggcgttgc
     4981 gataataatc tcattaaatt cactcactaa aatgaacttc tcaattgtat ggattaaaat
     5041 cggtttatta tcaatatcta aaaattgttt tggtaatggc acgttcccca ttctcgaacc
     5101 aatacctcct gctaaaatac ctgcataaat catgttgtcc tccattctgt cattacatca
     5161 tttccattta tacattactg acctatgccc gcacataagc ctaacctatt gctcacttgc
     5221 ctcttttatt aatccaaaga tagttgtcac aatagtgtga taatttttta taaaaatgta
     5281 tttttgtaac tgaccattct aagttgtttt gccatgcagt taatcattaa ctctgacgat
     5341 attaaattgt taaaggtatt aatgtttact ctttttcaaa ttcattatta ctgccatcat
     5401 tttaccatat attataataa atttatctta ttaagtggct gtacttgatt ttcactttaa
     5461 aaattatcaa atattgccat ctcattttaa gtatacaaaa tgcaaaacaa ccgattcaca
     5521 agcatatttc acacaagtaa accggctatt tatcaacgta tattcgaaga tgaattattt
     5581 cgatagtatc tgtagaccag acggcattcg cactttcata gctataacta taccagcgtt
     5641 ttcgtcctca aaggtgcata ctaataaatc gtaaacatga ctttatcaaa tcgttctttc
     5701 ttgttaacta atttatcaaa tgtctccggg cctttttcta acggtaaaaa atgagaaata
     5761 ataggcttta cattaatatc tttcgtcttc atataatgta aggttgccgt ccactctttg
     5821 cccggaaaat tactggacaa acagttccaa gagccacata ctgtcaactc gttacgcaga
     5881 attttttcaa aatgaacgcg atcaatctca atatcatcat atggtattcc gagtaatacc
     5941 acctcgccac cttttttagg tagcgtcaat atttgaccaa tcgtaacttt agcacctgat
     6001 gattctatag ctaaatcgat ttgattggcg taatgatttt cgatgaattt ctcaagattt
     6061 tcttcttttg aattgattgt ttgatgtgcg cccaatgatg ttgcaatatc tagtttatgc
     6121 gcatctatat ctatagcgat gatatgtgca gcaccaaata ttcgtgccca ttgaatagct
     6181 aacaaaccta tactgccaca ccccattact gcaacagtca taccaggttg tatattcgat
     6241 ttataaaacc catgcgcaac aacggctgat ggctcaacca ttgctgcttc aatgtaatca
     6301 acattgtctg gaacctttaa aacattttgc gctggcaatt tgacatattc cgcgaacgat
     6361 ccaggttcat atgagccaat gacgaataac ttttcacatc gtgcatattc acctttcaaa
     6421 caatactcgc attgataaca aggtattgct gggcaacctg tcactttgtc gcccacatta
     6481 acatgcgtaa catcacttcc aatggcatct actacacctg aaaattcatg accaaatggc
     6541 atacctttaa tgtatggccc catttttttg tatcgtgacg tgtctgaacc acatatgcca
     6601 gtcgctcgta ctttaacaat aacgtcattc gcactttcaa tgactggctt ttcattatcc
     6661 tcataccgta aatcttctac gccatataat ttcaatgctt tcacttgtaa atcacctcaa
     6721 atttgattta attcacaact tttttctttt taaaaatacc tgtcgcaaaa taacctgcaa
     6781 tgacgatgga attacttacg agtaaatgtt ccatataaaa atcagtgatt tgtcttaatg
     6841 gcccaagcat aaaagttagc attgattcta ccatattaat tctctccttc attttcatct
     6901 aaatcgagcg gcttaagtaa tgctttaata ccttcgccag attttaaatg ttgatacgcc
     6961 tcgtcccatt tcgaaatatc atatattttt gtcaccaaag cttcagcatt tactaaacca
     7021 tccgccataa gttgcaatga aggttcccaa tctgctggct tttgacttct actaccaaca
     7081 actgttattt ctttttgaat cactttttcc atatcaaatg gaatttcagc atccttaaaa
     7141 atacctattt gactgtagaa accttttttg cgtataatat ctaaaccttg tcgtgctgct
     7201 ggaactgcac ctgaacattc aacaacaaca tctgcaccgt aaccgtctgt aattccattg
     7261 atatacgttt ttaagtctgt ttgttgtaaa ttgactacat aatccatgtg caatgcttct
     7321 gctttatcta atctgacttt gtcattgtcc aatccagtta ccacaacagt tgcgccttta
     7381 cttttcaaca cttgtgctac aagtaatccg attggcccag gtcccattac aactgctaca
     7441 tcgccagagt tcacttgaat cttagaaacg ccatgatgtg cacatgctaa tggttctgtc
     7501 atagctgcag actgatacga tacttcgtct ggaatatgat gcaaactttc ttcacgtgca
     7561 atgacataat tagtaaatgc gccatcaact tgtgttccaa taccttttcg atggttgcat
     7621 aaattatagt cttttgattt acagtattca cactcattac aaacatagaa tgtagtttca
     7681 gaagtgacac ggtcaccaac tttaaaatct ttaacgtctg ctccaacttc aacgatttca
     7741 ccagaaaatt catgacctaa tgtcactgga aaattaactt tataatgccc ttcataagta
     7801 tgaatgtctg tgccacaaat tcctgcataa tgtactttaa tctttacttt atcatctagc
     7861 ggtgttgcaa cttctttatc aagaagttct aagttgccat gtccttctct tgtttttact
     7921 aaagctttca ccacaaacac ctcgattttt aattgaatag actaaatagt ttaaagataa
     7981 gatagttaac gatattacca ccttgatcaa tacttgaaat ttcagatgaa ccttttggca
     8041 tttgtacatt cgtacctttc gccatatctg tgaaaatggg tgctacgtct gttgcaatat
     8101 ataatgaaat tgcaatcata atcgtaccca caatgacaga atgaataatg tttcctcttg
     8161 ctgcaccaac aataaacgcg acaacaaatg gtatagttgc taagtcacca aaaggtagta
     8221 cttggtttcc tggtaaaata acggctaata aaacagtgat aggtactaaa attaatgctg
     8281 tcgaaataac cgctggatga cctaatgcta cagccgcatc caatccaata taaatttcac
     8341 gttcgccaaa acgtttattt agccatgttc ttgcagactc tgaaactggc attaaacctt
     8401 ccattaagat ttttaccatt ctaggcatta ataccattac tgcagccatt gacattccta
     8461 aattaatgat gtctccaggt ttgtaacctg ctaacacacc aatacctaaa cctaaaatta
     8521 agccgacaaa tatagactct ccaaatgcgc caaaacgttt ttgaattgtt tcaggatcag
     8581 catctaactt attcagaccg ggtacttttt gtaacaattt aactaagtaa atacctggtg
     8641 cataagaaat cgtgcttcct gtcgcaatcg atacacctgg taaatcaaag aactcactca
     8701 tcatcggtgc tgtccaatct gctactttca aacagataac ttggaaaata actgctgcta
     8761 ataacgcttg ccaaatactg cctgatacgg cataaaccat tgctgctgta aacgtataat
     8821 gccaaaaatt ccaaatatct acattcatcg tctttgtcac tttagttact agcaatacaa
     8881 cgttaactat gattccgagt ggaataataa atgctgcgac agatgatgcc caagcgatag
     8941 atgatgttgc tggccaacct acatcaatca cattcagact gacgcctaaa tttttaacca
     9001 tcgcttgtgc tgctggccct aaatttttaa ctaataaatc gatgactaag aaaatcccta
     9061 caaaagccac acctattgtt aaaccagacc taaatgccgc tccaattttc tgcctaaaga
     9121 ataggccaag caagaatatg acaaccggta aaataacagt tgcacctaaa tctaaaaatc
     9181 cccttacaaa atcagtgaag taactcatat ttaaaccctc cctgttatat atgcattgtc
     9241 acgatacttt ccgattgtga ttacatttga cgttacggtc atttcaacga caacccttgc
     9301 tacattcgac tgcagtccct ttgaattaca gtcactgcgt ttctatgtca tcaacaatca
     9361 tttgtcgtga tagtcattta tatgcaattt gcatatattt atatgttatt gacccacgtt
     9421 acatatcaat tccgttattt ttgtaactct gttaagattt gttgttttgt ttcttcaata
     9481 ccaataccag ttaagaaatt acgtgcgttg ataactggga atttatattc tttctttgtc
     9541 attgcagttg taactaataa atctgcagtg tcttcataag gtccaacttc tgtaattttg
     9601 atttgtttaa tatctacttt aatattgtgt tcctttgcca tttcctcaat tgcattattt
     9661 actactgttg acgttgcaat acctgcacca cacgctacta atacttgttt cattttcaat
     9721 tcctccaatt aatttttagt tatattccaa ataatcattg attagtgttg ctaaaatcgt
     9781 ttcatctttc gttcgcagaa tctgctccaa tttttcttca ctttgaaaaa tttgcatcaa
     9841 ctgttgtaac aacttaagtt gatcatctac tttatccatc gctaacataa aaacgatttt
     9901 cacttctgtc tgttgatcaa gtgttcccat ttcaataaac ggcacctctt tttctagaac
     9961 agccacacct atcgttctat ggttaatatg ttcgacatct gtatgcggta tagcgaccga
    10021 acatagatgt gttggtaaac cagtagcaaa ttctttttct ctgtcgatga ctgcatcttt
    10081 aaacgttgac ttcacgaacc cattttgaaa taacacatct gacatttgtg acaatacgga
    10141 ttctttatca gttgccgaca aattgagcat tatattttct ttatgcacta attgctgtcc
    10201 catccatttt ccctcgcttc tttatttgaa taatttttta aaatctcatt tacatcagaa
    10261 tttttgcgac tttgtatgat gcgcttaatt gcgtcattgt cttgcgccac atctctcaat
    10321 tgtagtaacg ctcttaagtg tgtcacttta tcaacagcag caataggtac aataatatgg
    10381 attgctgtgc catctgacat gtatattggt tcttgtaata tcaacatact catcgctgtt
    10441 ttatgtacat gcttttcaga gtatgcatgt ggaatagcaa tattttgcat gataaccata
    10501 tacgaatcat caaatacctg ttgcatctca tgaatatatt taatatcaat aaaatgatta
    10561 gcaactaaca catcacttgc tttagcaata gcttcatcaa tattttcaac atgatgcatt
    10621 cttttcacgt gccttgccgg tatcaagtca gctaaatcta atgtcttatt ttgtgtgaca
    10681 atcgatccat taatggttga aattgaatta taattggcaa taaaatcttc taaaccatca
    10741 cgtagttctg taatgtcatt aactgtcgtt gtgcgttcaa ttaatgccat taacttgttt
    10801 atttccttat caatgtcagc cgattcctta ttaatgtact tcatcacttc tttacgtaac
    10861 tttcgttgct cattttcaga taaagctact tttgtgataa ataatttttt atgtgttagg
    10921 acaaacattg gtgaaaagac gatgtcataa tctaatgtgt aattttcaaa tgttctaagt
    10981 gaaatcgcat ctaagaaaat aatttctgga aataagtttc gcaactcgta taacatcatt
    11041 tgtgatactg acgtgccttg tgtacacacg ataatagctt ttatcttgcc atcgaagttt
    11101 tcatcttgac gtctcaaact acctccgaac aacatggtta aatatgctat ttcattatca
    11161 ggcaacgatt ttccgaaata ttcagttaac gattgacatg attgtttcac catatgaaat
    11221 aaggattgat aatttccttg taaaggattt attaattcat cacgatccgt taagttatat
    11281 ttaatcctat aaaaagcagg cgttaaatgt aacaagagtt gctgtgataa tttctcctta
    11341 tcttcaatgt taataaaagt gatttgttca aaatggtgaa tcatttgagc gatggccatc
    11401 gttaaattcg atatgttatc tgattcctgc aaatcagtcc attgcacact tgttgaaagt
    11461 aagtgtaatg tcaaatataa cttttccgct tctggcaaat caggctcatg ttgcgtcata
    11521 atctccgttg cttgatattc tttcgtatcc ctcaaatact gataattaat atttaatgga
    11581 ttcatcacat gaccactttg aattcgtcta cgaatcacac aaaggacata aggcaatgaa
    11641 ctaagtgatt tgtctataaa gcgactcttc aaaaattgtt ctacctgttt gatcttgtct
    11701 ttttgatatg cgatatcttc gaatgttaag ttgagcgcct ttaaaacttc acttttagta
    11761 atatcatgat tcaacctttg atcaatcaac ttaatgaaga aacggcgaac ttcaaattca
    11821 tcaccaacaa tttcataacc atgctttcga gaatacttaa gtgacaaacc atgatcttcc
    11881 aattgctctt tcacatgatt tatatcgtga atgacagtat ttttactgac ttgtaaatca
    11941 attgaaaaat ggtttagaga cattgcattt tccttactaa aaagcatgag cattaaataa
    12001 taacgacgtg tttctatgct aaaaatgaca ttgttgccgt ttaacatttg ctgctccgat
    12061 acatctcgct tgaataacgt catgatttca gaacttacaa taaaatttcc ttggcttgtt
    12121 ctttcaagtt ttggataacc ctcttgttca agccacaaat tgattttttg aatgcgatat
    12181 cctagttgtc tacgagacaa accaaatatc gattcaagtt ctttaccatg aatagtagga
    12241 ttcaatacaa tttctctgag tatttgctga ttatccccgt tcatgttcga tcctcctttg
    12301 agcatctgag ctcaatttat catcaatcgt cacaccgatg tacacacttt gaacacatat
    12361 ttccaaaatg agcatgtaca tcattgtgat gttttaacaa catttcaatt atatctatat
    12421 tttttgtgat tttaatcttt taaaataaag caattgaaat ttttacatat atttttgtgt
    12481 tttatgtttt ttgaagcatt tttaacatac atatctcaat cattatcaaa ttgtcatgac
    12541 caatgtaatc cactataaaa accctaagga cgcttatatc aggcgcctta gggttaactg
    12601 tatctattta attaagtatt attattcgta tgtacgtaac ttatggtcta tcaagttcca
    12661 cacttcttca acatcaactg ctgtagcaaa ataagcattg gcaggcttac ctgtaacatg
    12721 atttaaatcg acagccatag tgccataagt tagtggactt tgatgttcaa tgtcgatatt
    12781 aacgggtacc attgtaaaca attctggttg taacaaatac aaaattgtac aagcatcatg
    12841 tattggacca ccatccatat taaagtgagt cttgtatgtc ttcttaaaga attgcaataa
    12901 ttctacgacg aactgtgcaa caggattatt gatactttca aagcgttcaa tcacgtgatt
    12961 gtcggctaaa acttgatgtg ttacatctaa accaaacaca tttatagtaa tcccactttc
    13021 aaaaacacgc ttcgctgctt cagcatctac ccaaatattg aattctgctg taggcgtcca
    13081 atttccaaat gtaccaccac ccatcaaagt aatagattca atatgctcag cgattcttgg
    13141 ctcacgaatc aatgccgttg ctacattcgt aagaggacct gtcgctacaa ttgttacagg
    13201 tgtatcactc gtcatcactt tgtttataat cacatctgat gctggcattg caactgcttg
    13261 acgtgatggt gtcgacggta gtttcggacc atctaatcca gattccccat gtatttcaga
    13321 agcaaaggca gctggtttaa ttaacggcct atccgcacct ttcgctactg ctatatcttg
    13381 gcgtcccata atatccaata cgttcaaggc gtttgtcgta ttcttgtcaa ctgattgatt
    13441 acctgcgact gttgttacag ctaatatctc tagtggactg tcaattgccc ccgctaaaat
    13501 taatgctatt gcatcatcgt gtcctggatc acaatccata ataatctttc ttttcattta
    13561 tatatccacc tttcttaagt tgttatcgat agcttatgta tatttattta tgtggtgaat
    13621 catgtttatt ttgaaaaata gttttaactt tctcatattt ttggatacaa acactattta
    13681 tctattttat ggcttataaa tttatccgat atgccttatc aacctacctc gctaaaaata
    13741 ggatgtctac atatctatac cgacttttgt caactcattt tcacaacaat ataaacagca
    13801 atttatatga ttgttacatg attcaaacaa tttttatgaa aaatattttc atacacagaa
    13861 tatatattga tattaaattt ctcaaaagct atattgagaa taattaggag ggatgttgat
    13921 gaaatcttta tttgaaaaag cacagcagtt cggcaagtcc tttatgttac ctatcgcaat
    13981 cttaccagct gcaggtctat tgttgggtat cggtggtgca ttaagtaatc caaacaccgt
    14041 taaagcatac cctattttag atattacctt attacaaaat atttttacat taatgtcagc
    14101 tgcaggtagt attgttttcc aaaatttacc ggtcatcttt gcaattggtg tcgcaatcgg
    14161 attatctaga agcgataaag gtactgcagg tttagctgcg ctgctcggtt tcttaattat
    14221 gaacgcaact atgaatggct tattaactat cacgggcaca ttggcaaaag atcagcttgc
    14281 acaaaatgga caaggcatgg tgctcggtat acaaacggtt gaaaccggtg tttttggcgg
    14341 gattatcaca ggtattatga ccgcaatact tcacaacaaa tatcacaaag tggtattacc
    14401 accgtattta ggtttctttg gtggctctag atttgtccct attgtcacag catttgccgc
    14461 aatcttttta ggtgtattga tgtttttcat ttggccaagc atacaagccg gcatttatca
    14521 tgttggtgga tttgtaacga aaacaggtgc catcggtact tttgtttatg gcttcatctt
    14581 aagattgtta ggtccactcg gtttacacca tattttttac ttaccgtttt ggcagacggc
    14641 acttggtggt actttagaag tcaaagggca cttagttcaa ggtacgcaga acatcttctt
    14701 tgctcaactt ggtgatccag atgtgacgaa gtattattca ggtgtgtcac gctttatgtc
    14761 aggccgtttt attacgatga tgttcggctt atgtggtgcc gcacttgcaa tttatcacac
    14821 agctaaacct gaacataaaa aagttgtcgg cggtttaatg ttatccgctg cactcacttc
    14881 atttttaaca ggtattaccg aacctttaga gtttagtttc ttgtttgtcg cacctattct
    14941 ttatgtaatc catgccttct ttgatggatt agcatttatg atggcagaca ttttcaacat
    15001 tacaattggt caaaccttca gtggaggctt tatcgatttc ttactctttg gtgtgctaca
    15061 aggtaatagt aaaacaaact acctatacgt catacctatt ggaattgtgt ggttctgttt
    15121 gtattacatc gttttcagat tcttaattac gaaatttaat ttcaaaacac ctggtcgaga
    15181 agataaagct gcagcacaac aagttgaggc tactgaaaga gcacaaacta ttgttgctgg
    15241 tttgggaggc aaagataaca ttgaaatcgt tgactgttgt gcaacgagac tacgcgtcac
    15301 acttaatcaa aatgacaaag tcgataaagt attactcgaa agtactggtg ccaaaggtgt
    15361 aatccagcaa ggcactggtg tgcaagtaat ttatgggcct cacgttacag ttatcaaaaa
    15421 tgaaattgaa gaattgctcg gggattaaga ctaaccgaaa tatcaacaga actaatggca
    15481 acgatgtacg aagtaagaag tgacatcgtt gcttttattt ttaatgttac atttgaagca
    15541 ttaagttcat catacactgt agtgagcccg caaatcgcct ctgctagaca atcatcttaa
    15601 tgctatgatt aaagcttaag tgccagattt gaatttaatt tcaacaacga ctttcactac
    15661 attaaaaata gggctactcg acacatatag ttgtatcaaa tagcccttta tacaattttt
    15721 tgggtaaggt tttacaattt ttggaatggt atagatttta taaaaagtta tttaagttct
    15781 tctgcttcag ccataatatc ttttaatgtt ttagctgaat gtgcgaactt gctttgttct
    15841 tcgtcgttta atgggatttc tacgacgttg cgaataccgt ttctattgat gactgctgga
    15901 acaccaatat aaacatcttc ttcatcatat tcgccttcta ataatgctga tacagtcaat
    15961 acggcatctt catttctgaa aatcgcttca gtaattctag ctaatcccat tgcaacacca
    16021 taataagtgg cacctttagc ttgaataatg tcatatgctg catcacgtgt ttgaacaaaa
    16081 atttgttcaa tttgcgcttt gccctcagga cgttgttcaa gtaatgtctt caaaggttga
    16141 cccgcaatat tagcgtgtga ccatactggt aattcagtgt caccatgttc accaataatt
    16201 tgagcatcga cgctacgtgg cgcaacatcg aacgcttcgc ttaacaataa tctaaagcgt
    16261 gcagagtcta aaattgtacc agaacctata acacgttctt taggtaaacc agagaatttc
    16321 catgttgcat acgctaaaat atcaacagga tttgtagcta ccaagaaaat accatcaaat
    16381 tttgatgcca ttacttcacc aacaattgat ttgaatattt tcaagttttt agatactaaa
    16441 tctaaacgtg tttctccagg tttttgtgca gcaccagcac agatgacaac tagatccgca
    16501 tcatgacaat cactgtattc gccagctttc acacgaactg ttgttggaga atatggtgtg
    16561 gcatgtttta aatccataac atctcctcga actttttcag tgtctaaatc aatgatgact
    16621 aattcatcaa caatgctttg gttcactaat gaaaatgcgt agcttgaacc tactgcacca
    16681 ttacctatta atacaacttt gttcccttta aatttgttca ttacaaaaac tcccttatga
    16741 ttaattcact aacatacgtg tagcttcaaa tatgttagtt taatgctgct tattgacgat
    16801 acaaaagcaa ataaacatct cttttatttt caacgcataa cttaaaaggt catgtgtcat
    16861 ccgcttttaa gtttgtgatt tatttcacat ataaaatgta acatgcatta agtactgggt
    16921 caatattaaa ttgtgattta tttcacattt tattttaatt tttacacctt tttaatttgt
    16981 atacgattac atcttaggtg tctttagtct tcgtacttcg ccagtgatta tttacacttt
    17041 cacattttta ttatcatgtt tacttttttc taagaaaaca acaatgtttt ttgaattagt
    17101 caaataaatg cgcgcaatcg tcggtgtgca aacagacaat tgtacacaat gcttattgat
    17161 aagtatttaa aaaattaaaa atgtcataca attatcaaat ttgccatttt atttatattt
    17221 tctcaaacca attaattgaa tatcgaaatt tttagtagaa taatcaaaat agacagatta
    17281 aaggaggagt atcatgctta cagaacaaga gaaagacatt atcaaacaaa cggtgccttt
    17341 acttaaagag aaagggacag aaattacgtc aatcttttat ccaaaaatgt ttaaagcgca
    17401 tcctgaactt ttaaacatgt ttaatcaaac gaaccaaaaa cgaggcatgc aatcttcagc
    17461 attagcacaa gctgtaatgg ccgctgcggt taatatcgat aacttaagtg ttattaaacc
    17521 agtcattatg ccagtcgcat ataaacactg tgcattacaa gtttatgctg aacattatcc
    17581 aattgtgggg gaaaatttat taaaagcaat ccaagacgtg acaggtttag aagaacatga
    17641 ccctgtgatt caagcttggg caaaagcata tggtgttatt gcggatgtgt tcatccaaat
    17701 tgaaaaagaa atttatgatc aaatgatgtg gattggtttt aaaccattca aaattaccaa
    17761 tattaaacaa gaatctgaag acattaaatc atttacagtt gaaactgaag aatatgactt
    17821 tagtgaattt acaccaggcc aatacatcac agttgatgtt tctagtgata aacttccata
    17881 tagagctaaa cgtcactatt ctatcgtatc aggtgaaaaa aaccatttaa cttttggcgt
    17941 taaacgtgat gtcacaacag aacatgaagg cgaagtttca acaattttac acgatgagat
    18001 taaagaaggc gatatgatta atttagctgc gcctgtaggt ggattcgtat tagagaatac
    18061 gactgaacca caacttttct taggttcagg tattggcgtc acacctttag tagcaatgta
    18121 tgaagctgcc tctgccaaag gtttagatac acagatggtt caagttgctg aaaatgaaca
    18181 acatttacct ttcaaagaca acttcaacag tatcgcaagc cattatgaca acgctaaatt
    18241 atatacacac ttaaaagata aacaaggcta tattggcact gaagaattac aagcattttt
    18301 agcaaataaa cctgaaattt atatctgtgg tggtacaaaa ttcttacaat ctatgatcga
    18361 agcacttaaa tctttaaatt acgatatgga tcgcgtacac tacgaaacat ttattccaag
    18421 actaagcgtt gcagtataac tgagaaaaga ggtttagtgc tcatatgaaa cttaatcaac
    18481 gttacgtaaa agtatttgca ttatatttcg taagtattgt tactgcaaat attattgtta
    18541 aaaataataa tttaattaaa acattgatac aaaccatagc cgggtacacg gtctttgcag
    18601 ttggtttgaa gtatttaact aaacgtaaaa ataaatgaca tctataccaa aacagctatg
    18661 actttacttt gtcatggctg ttttttaatt gggagtatga cagagatgat attttcgcaa
    18721 aatttatttg gtcgtcccac cacaacatgc attgatgtat gctcactgaa tttcataaga
    18781 aaggagttca caagatgacc gtagatattg gacggattta tgacaataaa gataataccg
    18841 acgctattcg tatcctagtc gatagagtct ggccgagagg tatttcgaaa agaactgcta
    18901 acctagatta ttggttaaaa gacattgccc cttctactga gttgcgacaa tggttccaac
    18961 atgatcctaa actttttgga gcttttaaag aaaaatatga aaaagaatta cgtgatcagg
    19021 atgcgcaaaa agatgctttt gaaaaattaa aggatattgt aaatcagcat aatcatgttc
    19081 tattgttata tgcagcaaaa gatactaaac ataaccaagc tgtagtacta cagcagttgc
    19141 tcaatactta gttatatatg tttatcgtga atcactataa atgtcgctaa cttcatttct
    19201 aatatttatt tttaaaaagc ataattacta caattaattg aactttaata attactaaac
    19261 ttgaacaaca ttttacttta acaaaataaa gttttaaaat attaattgtt gggttttaca
    19321 tagtaattag aaagagagtg ttaatgaaat gaagtttaaa agactagcaa ctatattttc
    19381 agcagtatta gtgttatcag gatgtggctc aatgcattca tctggcaaag acttaaatat
    19441 ttcgttaccg ttaaaaacaa agtctattgc accttatgaa acagatgtac cagttaagat
    19501 tggtgctgca gaatcattat ttaaaacaaa tgatcaagga aaaatagaaa aagcattagt
    19561 gaaatcgtat catcaaccaa atgatacaac gttagatata gaattaaaag ataatattaa
    19621 atttcaaaac ggtcaaaaat tgactgcaga aaaagtgaaa tctagccttg aaaatagcat
    19681 gaaaaaaagc gacttggtca aatattcatt accaatatca tcaattaccg ctaaaggtca
    19741 aaaactgaca attaaaacca actccgctta ccctgaactt gtatctgaat tagctaatcc
    19801 ttttatggca atttattata cagatgctaa atcagatgtt aatcaaactc ctgttggtac
    19861 aggcccttac caaataaaag attataagca atctcgaaaa atatcattgt cgaattttaa
    19921 ggactattgg caaggtaaac cgaaacttga tcatattact gtgacgtacc aagaagacgg
    19981 caataatcgc gtcagaaatt tagaatctca aaaagatgat ttaataactg atgtcccagt
    20041 taataaagtt catgacatag aaaataatca aaatttaaaa gtgtcaaaag aatctggatt
    20101 tagaacttct ttacttatgt ataatcatac taataaaaaa atgactaaat ccgttcgtga
    20161 agcattagat catatcattg atagacaagg tattgcagat catatttatc aaggttatgc
    20221 gaaacctgca acgagtccat ttaatgataa aattccatat attaaagaac ctaagttaac
    20281 caaacaaaat atcgaacaag caaaaacgtt attagctaaa gatggttata caaaagaaca
    20341 ccctttaaaa attaagttaa ttacgtatga cggtcgtcca gagctatcga aaattgcgca
    20401 agtattacaa tcagatgcta aaaaagcaaa tattgaaatc gacattaaaa gtgttgatga
    20461 catagaaggt tacttaaaag accgttctgc atgggatgca acgatgtata gtttcggaac
    20521 aatccctcgc ggtgatacag gttatttctt taatcaagca tataaaaaag atggtgctat
    20581 taataaagga gactacaata atagcaacgt cgatgattta attaatcaat tgaatcatac
    20641 tgtagatgtt aaggaacgtc acaatatttc taatgatatt attaaattat caagtcgaga
    20701 tgtaccaaac agttatattg cttacaacga tcaaatagtc gctgctaact ctaaggttaa
    20761 aaattataaa gtaacacctg aaggtatcta tttaatcgat tatcgaacaa cgattgaacg
    20821 ataagggagt gtgggacatt gatttatgtc tcagccctgc ttcaactata aaaaccacgc
    20881 cgaagtcatc caatatgatt atggcgtggt ttaatttgct tatatgattg tattaatttc
    20941 tgtttgatga atttcaataa aatgattatt acatactttc aatgaatcat gttcatttta
    21001 tataaatcta attgtcacta tttgtttaac gtgaaacact tttttaactc caaaaagtat
    21061 tcctattcca ctcttttaaa gacaaattat aaaaatagct tatgtcacaa acatatgagc
    21121 aacataagct ttaattatta ttcgtctact tttttctgtt gttctttaaa ttctttctgc
    21181 agtaaattca cttgttttaa acctgccccc attaaaatga atacaagtat ccacacaata
    21241 acgattaaaa taaatatttt caacgtatca ctgccaagta cagttaaaga tgtatcccaa
    21301 atgccatgta aaccaacggc tgataaaaag aatattaaaa agcgtttatc aaaaatatct
    21361 ttgaattcaa agccatgctg ttctttcgca ataactattg cagcaccaac aatcgctgac
    21421 caaactaaat gaccaccaat cgcagtccac gcacgtgtga agacaatatc taataatggg
    21481 acattttccc ctaaagcgaa attcaaaata taacctgctg attcaaaaac tgcgaaccct
    21541 gcaccaatag cagcaccgat taataatcca ttcaaaatct tatttgtttt caatttattg
    21601 acgaaataaa caataatgag tgcttttcca gtttcttcaa ctaatcctac taaaaatgca
    21661 tcgaaaaatg ttaaagaacc aaacctttcg aattgatcac taaaaacgac aaatctatat
    21721 aataccatcg tacttagtaa tgaaaatacg ccgccaataa agaacatgat aataacttca
    21781 aaaatgctaa tatttttaaa cgcatttgat tcataaaaga agaacaaacc cgataatggt
    21841 actgttaaag cccctataaa aatgagaccc ggaatcgcgt tagtgttatt aaaaatcgct
    21901 gccatgaccc ataatccaat aaatgttact gtgaaagcga tgaatactcg agaaaagagc
    21961 catggcttcc cccattcttc tgatatgtca caaattgctg gcgtagtttt agcagtaccc
    22021 gctataaata tttcatctgc ttcgttttta gtatgcggct taaatacttc agaaaacata
    22081 tcttttaaat taagatttaa gctttcatca ttgccggcta gtctaccgat tgatttggtt
    22141 gcattatcaa ataaacttct tgcttcggtt tttaaatctg cattttgcgc tgtttcatgc
    22201 gctttggctt ccatttgttt attgatagat tcattatgta tgtcggacgt cttagttgat
    22261 tgtgtacttg ctgatgacat cccctcttca cctgtattag gtgcaactga ttgcgtttgt
    22321 gttgtctctt ctttttctgt atttcttcga tgattaagac cttccataga cttacctact
    22381 ttcacttttc ataataaaac tattttaatt taatattgat ggattttcca tattttaata
    22441 taaatacttt gttcgattgg gcgctttgta tttcatattt aaaactcaaa acctatttaa
    22501 ctttttatat tccgagcaat atttttatat aagtatttta atgatattca attcattaca
    22561 aacccttgat attacaattg ttatcaacca ttaaaatgtg tctatactgt gtctattatc
    22621 agaataaaaa gatatttcta attgatttta aacgtcgtta tgttatattt cttgtaaggg
    22681 tttacacaaa gtgtaaaaac gctatatttt atcaaatgtg atggaggtga cttgcttgaa
    22741 acaaatcaca tggcacgact tacaacatat cattaaagat ggtgatgtga ttggtttacc
    22801 agcattagct gtagccaact tacccgccga agttctacgt gctgtgttag cgcaacatga
    22861 cacatatcat acgcccaaag atttaacgtt tatattagcg aatgatatcc atagtttagg
    22921 tgccgcaccg gatttagatg attttataga acgtcgcatg attaaacgtg tcattatgag
    22981 cattttaacg gcttcttcca aaacggcaca agcaatgaaa aataatgaca ttgaagctta
    23041 ttttttacca caaggtatca ttgcaactca ttatcgtcag agtaatcaat tattacctgg
    23101 agttattact aaaatcggat taaacacagc tgttgatcct agatacggtg gcggtaaagt
    23161 aaatacacga acaactgatg atttagtttc attagtaacc atcaacgatg aaacatactt
    23221 acattacaca ttccctagcg ttgatgtggc actactgaga ggaacatacg cagatcaaca
    23281 aggtaacatt tatttaactc aagaagcgta cttgagcgag tgttatcatg tcgcattaaa
    23341 cgcgaaagcc aatcatggga aagttattgt acaagttaaa gctttagttg acgactatca
    23401 actaaaaccg aatgaagttg ttatcccagg aaatcttgtc gattatgtat acgtcacaga
    23461 agatgaaaag aatcaccgcc aagtaattca gagtcattat ttaccagcct tgtctggaga
    23521 agaacgaatt gatggaatac ctgaacccgc attacctttt aatagtcgca aattgattct
    23581 ccgacgtgct gctcagtttt taacttatgg cgatacaatt agcatcggtt atggcatcaa
    23641 taatgaactc tctaatttat tgcacgaaga atgtgttgaa catgatgtgc aaccgattct
    23701 agatgttggc attttcggtg gattcgttgg gagtcgtgaa cattttggta tgaattacaa
    23761 tgcagatgtg cgcatgcctc atgatcgagc atgggatttt atttataaca atggtgtatc
    23821 agttgcctat cttagctttg ctgaggttga tcaatacggc aatgtcaacg tgtcttactt
    23881 caatgaccga ctaaatggat gtggtggctt tatagacatt acgcaatctg taaataaaat
    23941 tatcttttca ggtacttttg tagctggcag tcatgtctca tgtcataatc aacgattaaa
    24001 cattgaaact gaaggacaaa accagaaatt tgtatcagat gtgagccata tcgactttaa
    24061 tgcacaatat tcacaattac tcgagcaaga agtctatttt gttactgatc gtgcagtatt
    24121 cgagctcacc aatcaaggct tgaaactaat tgaaattgca ccaggtcttg atttgcataa
    24181 agatattttg aatcaaatgg cttttaaacc aattattgct gatcatttaa aattaattga
    24241 taccagcatt tacaaagaaa aatggggaca acttaaacaa tcaattcata aagtatgaaa
    24301 gtgaagctaa agggggtata ctaatgaatt tcgattggat aaaaacgcgt tcagacttcg
    24361 atgatgacaa gcctgccgtt attgatcacg caaaacaaac atcttggaca taccaacaac
    24421 tcaatgcacg cgctgataat atggcacatt atttaacatc tcaaggtgtt aaaaaaggcg
    24481 atgttatcgg tatttttgcg ccaaatgata ttgcaatatt agatttattg tttgcttgtt
    24541 ttaaaacagg tgcggttttt ttaccattga attggcggct taacccaaaa gaaattgcag
    24601 ccattgtgga agatgcacaa ttaaaactgc tcttctatgc tgaaaaacat ttaagttcac
    24661 tcaccgatat tgaccaaaac ttattgcata tggatattga tgtggcgcaa tatgatgaaa
    24721 tcgtaaatcc agattatcac caaccttttc aagcaacacc tgttgaacca caagatcttg
    24781 cagcattaat ttatacaagt ggtacaaccg gatcacctaa aggtgtgatg ttctcatatg
    24841 aatcatttgt tcataacggt gcaaacttag agctgacgta caagttcaat tcaaactata
    24901 ttacgattgt atcaacacca atgttccatg ttttagggtt taacgatact gtattaccag
    24961 tattaatgtc aggtggcaca cttattcttc aacgttactt taacggcgaa gaattgaatg
    25021 acatgattgc acaatatcac cctacattta ttatcatgat tccgacaatg tattatagta
    25081 cgctacgtgc tagtaatttt aatcctgaaa attttagagc tatggattat atcatccaag
    25141 gtggttcaca accattacca agtatccaag cagcttttaa acaatatggc ataaacatta
    25201 ttaacggcta tggtttaact gaagcacctc ttgtacttgt taatacaccg gaaaattcaa
    25261 aacgtaagcc aatgagtatt ggtaaagcag tcatgttcgt tgatgcacgt atccttgatg
    25321 ataacggtga ggaagtacct actggtgaga ttggcgaact tgcaattaag gctaaaaatg
    25381 tcacgccagg atattggaat aaaccagcag agactgccaa agcatttcat ggtcgttatt
    25441 tattaactgg tgacttagcg aagatggacg acgatggcga tatatttatt attgaccgca
    25501 aaaaagaatt aatcataact ggtggcgaaa atgtcttacc atctgaagtc gaaaatgctt
    25561 tagctgagca tccactagta gaccggtgtg tggtcgttgg ctatgatcat ccaaaatatg
    25621 gtgaatcaat tgctgcagcc attatacttc gcgaagatga acctcattac gctgaaattt
    25681 tagatcaaca tatgcgaagt cgtttagcag gttataaagt cccaagaatg tatgtaccag
    25741 tgacacatat gccgttaaac agtacgcaga aaccagataa acttgcgatt cgacaaatga
    25801 tgaatgacaa agtctcacaa acactttaaa ggtgataaaa atttttgaca tttagtgtaa
    25861 gcgtttacaa ataaagcgtg ttgtttttga attaaatgca tttcacatta gtattcatat
    25921 tatttttagg aggaatttat atgacatttg aaaaagaaac ggtcttaaaa acattatttc
    25981 ctgaagatgt acttagtatt gctaaaggtt taacagacgg tgaagtcgaa tttttacaac
    26041 aagtagattc attgctagaa agtaagtacc gtgaaaatat taatcaacat tggatagacg
    26101 ctactgtacc cgaggactat tttaaagatc tgggagaatt aaattatttt aacaatccat
    26161 tactttacaa ggatcgtcca aacgccaaaa tgcctagtca actatttcag tttttcatgt
    26221 cttacctact cgcgcgattt gatatttcct tagctaccct actcggtgtt caccaaggtt
    26281 tagggcataa cactttctat ttcggaggta gcaaagaaca aattgcgaaa tatgtaccta
    26341 aattacagtc acatgaactg cgtacatgct ttgctttaac tgaaccagaa cacggttcgg
    26401 acgttgcggg aggtcttgaa acagtcgctg aacgccaagg cgatacttgg gttatcaatg
    26461 gtgaaaagaa atggattggt ggtgcacatg tatctgatgt cattccagta ttcgcagtaa
    26521 ataaaaaaac tggcaaaccc cattgctttg tagtcagacc agaacaagat ggcgtcgata
    26581 ttgaagtcat tgataataaa atcgcacttc gcattgttcc taacgcacta attaaattaa
    26641 ctaatgtcaa agtagatgaa gcggatcgct tacaaaacat aacaagcttt aaagatattg
    26701 ccaaaattct ttattcaacg agagcaggcg ttgcttatat ggctacaggt ggtatggctg
    26761 gcgctttacg tgccacatta gattatgtca ctgagcgtaa gcaattcggc aaaccaatta
    26821 gtaaatatca gttaatacaa gaaaagctag caatgatgca aggtaattta gctcaagcaa
    26881 tggcaacatg tgctcaatta gctaatatgc aagcacatgg tgaatatgac gaggttgcaa
    26941 cttcaacggc gaagatgatg aatgccttac gtttgcgtga gacagtagct atgggccgcg
    27001 gtattacagg tggtaatggc atactagctg acgattatga tattgcacgt ttcttctctg
    27061 atgcagaagc gatttacacg tacgaaggta cacatgaaat taatgcctta gtaattggac
    27121 gcgctttgac tggagattct gctttcgtat aaatagcaaa taattatatg agatgcatta
    27181 atttcactaa aaaagactta ttttaagcat aaagcttttt ccttaaataa gaggctaaga
    27241 tgactgtcaa agatacttaa ttaattttat aaaatagcaa cgttattcca attatcttaa
    27301 tggttatctt atcctcaact aaattggagg aatcactatg acaattaata aagtaaccgt
    27361 tcttggcgca ggcacgatgg gcgctcaact ggcagcactc tttgtgaatg ctggacttaa
    27421 agtaaaacta ttagatattg tagtggacaa aaacgatcca aatctcattg cgaaaaaatc
    27481 ttacgataaa attacagata agaaacggcc gctactattc gacttaaatc tagtgagtca
    27541 tttaacatat ggtaattttg atgatgactt ggtaaatgat gatgctgatt tatatatcga
    27601 agcagtcaaa gaagatattg aaattaagca tgctgtttgg caacaagttc tacaacatgc
    27661 taaagaagat gctttatttg ctacaaatac atcaggtatt ccaattaatg cgattgctca
    27721 agcatttaac gagaaggatc aagaacgatt ctttggtcta catttcttta acccaccacg
    27781 tattatgaaa ttagtggagt taatacctac gtcacacacg aaggaatcta tcatattaga
    27841 tgtaaaaaat ttcgcgcaaa atgtattagg taaaggtgtc attgtcgtca atgatgtgcc
    27901 tggctttgtc gcaaatagag tcggcacgca aacaatgaat gatattatgt atcgcgccga
    27961 gcaacacaag ttaagcattg tagatgtgga tgctttaact gggcaagcga ttggtcgtcc
    28021 taaaacaggt acatatgccc tatccgacct agtcggttta gatattgcag tgtctgtaat
    28081 taaaggcatg caacaagtac ctgaagaaac gccttatttt catgatgtca aaattgtaaa
    28141 tacgttgttt gacaatggcg cactcggacg taaaacgaaa caaggatttt acaaaaagga
    28201 taaagaaact aaagctcgac ttgtttacga tgttgaaaaa caagattatg tacctgtatc
    28261 gcaaccacaa ttaccaattt taaatgaatt taataaagac ttagtgcata accttgatgt
    28321 catattcaat gcgcaagacg aagcgggact atttttatgg gagacattac gtaataattt
    28381 ctattactct gctatcaatg tacctaaagc taccgatgat ttccgagaca tagaccgtgc
    28441 gcttgtctgg gggttcaact ggaaacttgg tccattccaa ttatgggatg caatgggata
    28501 cgaacgtgtt aaaacacgta tggaagacga acttggagac ttaccacaat ggattagtga
    28561 tttagatggt ggcttttata aacaagatga gaccattgaa tatgcaacac ctgtttctca
    28621 cttcgtaaaa gatgaacttt gggataaagg tgatgccaaa ctttccgtaa ctcatgatga
    28681 tcaactgtta ctgaaattac aaagtaaaaa taatgtcatt accgatgaat tcaacgatgc
    28741 gttagttgat gccattgatt tactggaaaa tgaccattac acaagtatgg tcatttatgc
    28801 agatggtaat aatttcagtg tgggtgctaa ccttttctta atgaaaaagg cgcatgaaga
    28861 cggtcttgta gatgatgtcg ttgcacaatc aattgataaa ttacattata gctttaatcg
    28921 tttgaagtat agtttgaaac cagtagtcac agctgttcaa ggtcgtgcct taggcggtgg
    28981 ctgtgagctt gtactttact cacctattgt tgtcgctgca agtgaaacat atataggtct
    29041 tgttgaagca ggcgttggct tattaccgag tggcggtggc cttgcagaaa tggctgatcg
    29101 catattacgc acatcgcata agtttgatga caaacaagct tccatgacaa aagtactgac
    29161 gaatatcgca tttgcgaaag tctctacaaa tgcctttgag gcacgtcgtt atggttattt
    29221 acgtgataca gatacgatta ttttcaatac agcacaacgt gtcgaagttg cgctcaaacg
    29281 tgcgaaatat gaagcagaaa caaactatat tccgaatcct agacatcaat atatcgcttt
    29341 aggtgaagac ttcaaagcat tgatccaagg acaattagat gcgcaaagac gggggcattt
    29401 tattagcgac catgattatc atatcgcctt aaatatcgcc acaattttag cgggtggtga
    29461 tttaccaaga aatacattta taaatcaacg ttacattcaa tcgttggaga aaattggctt
    29521 tattgactta ctaaaatcta aaaaatcata tgaaagaatt gcacatatat taaaaactgg
    29581 taagccatta cgtaattaaa agatagtcat taagagagga tgataaccat gcaagaagca
    29641 tacattgtag cttatgggcg ttcagccgca gcgaaagcaa agcaaggcgc attattccac
    29701 gaaagacctg atgatgtcgc agccaaagta ttacaaggcg tattgaaacg tattgacgga
    29761 aaattcaata agaatatgat tgaagatgtc attgttggta cggcttttcc agaaggatta
    29821 caaggacaaa acattgcacg aacgattgca ttgcgtgcgg gattatctga cacggtaccg
    29881 ggtcaaacag tgaatcgcta ctgctcatca ggattacaaa ccatcgcgat tgcagccaat
    29941 caaattatgg ctggtcaagg agatatactt gtagctggtg gcgttgaatt gatgagtgcc
    30001 gtaccaatgg gtggcaacga gcccacaaac aatccaacct tacaatatga tgatataggt
    30061 gcgtcatatc ctatgggttt aactgctgaa aatgtagcat cccaatttga cgtatcacgc
    30121 gaagatcaag atgcttatgc tgtcagaagt catcaacgtg cctatgacgc acaacgtgat
    30181 ggtcggttca aagatgaaat tattccaata cgggtaaact cagttgaata tacaaacgca
    30241 ggaccaaaag tacacacaaa tatctttgac caagatgaat ttatacgccc tgacaccacg
    30301 atggaggcat tagccaaatt acgtacagta tttaaagctg acggcactgt gactgcggga
    30361 acatctgccc cactttctga tggtgcagga tttgtagttt taatgtctgg agataaagtg
    30421 aaagaactag gcgtgacacc tattgcacga ttcgttggtt ataaggcagt aggcgttgac
    30481 ccgaaaatta tgggtattgg gcctgcatat gcgattcctg aagtattgtc actcagcaat
    30541 ctatctgttg aagacattga tttgatcgaa ttgaacgaag catttgcttc tcaaacgatt
    30601 gcatctatta aagaagtagg tctagatata tcacgtacga atgtgaatgg tggcgctatt
    30661 gctttaggtc atccattagg tgctacaggg gcaatgttaa ccgcgcgttt acttaatgaa
    30721 atgggtagac gtcccgatag ccgttacggc atggttacga tgtgtattgg tgtcggcatg
    30781 ggtgcagctg ctatatttga gtatgtgcgt tagaatggtt gattttggat gaagcggatt
    30841 cgttaggtta ttgaatgaag taggctgaag ttgaagcggg ttgaagcaat ttcgttttat
    30901 taatgaagct gtgtgaaata tagtgattga acaaaaaagt ggtttaatgg gatggtggtt
    30961 atttccgttt tagaatttaa catttacacg tctaatttta atcattgttt taaattttat
    31021 gaatcgaagc cctttgattt aataatattt gctaatgcta gtaacttatc tgattgttca
    31081 tgtttaaaat aaagaaaacc actcacatca gtgtgtgttc gaactagact tgtaagttcc
    31141 agttcggtac gactttctaa agcaattatt attgctgtga ttgtcgtata tcacttagat
    31201 gtgcgtggtt tattttaata ggttagtaat atattaggtc atgttatgtt taagactata
    31261 atgaataaat aatttagaaa tatgcttccg attgttcgat gctttaattc agttagaagc
    31321 atcatagaat gcatgattac tgttgtatag atacataatt ctttgtattg actgtatgtc
    31381 tttggataga gttataaatt tattttgtta ctctaggccc atatgtcgca gtaccatctg
    31441 catgtgttgt tacgttatat gcgtttgttt cgcttggctt tttttgtgtc gggcgagcgc
    31501 catatgatac ttgaccattt gcatgtgttg ttacgttgta tgcatttgtt tcgcttggct
    31561 tcttgtatgt cgggcgagct ccgtatgata cttgaccatt tgcatgtgtt gttacgttat
    31621 atgcatttgt tttgcttggc tttttttgtg tcgggcgagc gccatatgat acttgaccat
    31681 ttgcatgtgt tgttacgttg tatgcatttg ttttgcttgg ctttttttgt gttgggcgag
    31741 caccgtatga tacttgacca tttgcatgtg ttgttacgtt atatgcgttt gtttcacttg
    31801 gcttgttttg tgttgggcga gctccgtatg atactgtgcc atcttgattt gtcgttacgt
    31861 tgtatgcatt tgtttcactt ggcttgttga atcttggtct cgcttcatat ccaaatgttc
    31921 catcgttgta ttcacggata cctgtaccag catctctata tttcacatac ttaggtgttt
    31981 tgttaaattg cggtctcgga ccatattgag aagcttctgt tgtttcagtt gcttgaggtt
    32041 taacttcaat atcacttgat tctccttgaa tacctttcaa cgttgattca gtaccttgtg
    32101 gttttatttc aagtttagat gagctacctt caagaccttc taaaataggg ttcggtgtcg
    32161 tcggttgagt ataattatcg cttaaagatg gtctgttttg ttccattgtt agaaaatcgg
    32221 gaccttgaac gatttcacct tgtaacgttt tattttccat cgttggatat tctggacctt
    32281 ttacagtttc accatagatt gtttcttgtg gaatttttac taatggctgt gccactggtt
    32341 gtgttgtttc ttcagcttta ccagccgtag ttttaacctc ttgctggttt ccaactttag
    32401 gtaattgagg ttcttcaact ttcttctctt cttttacaac aggagatttt gttacagttt
    32461 cctcgtattt tttgacagtt ttatttttcc aagattcgtc tgcttcttta actgcttttt
    32521 ttgtttcttc aactaattta tcaaaattag gtttattttc actcttctct ttaaaattgt
    32581 gttttgttgg atcatatttt gttatagaat tcggtctatt ttgtttagtc tccataaaga
    32641 aatcatctat aattgaattt aagtcatcga tcatttcttt ttttatacgc tcatttgtaa
    32701 ttttatgtgg attgtctgta tctccaagga ttaagtccag ttttgctcgt aactctttcg
    32761 catgctcccc ataatcctta tcagcataat aagttacaac taatgtatca atttcagaaa
    32821 caaggtcata aacttcttta gttgccttgt cttctccatc tttatcaaac tgcttcaagt
    32881 caacattttt atgctctatt tctttaactt ctttattaaa ttgataaact gcatctttca
    32941 aagagttaaa aatatcgtta tattcattca ttgttaaatt gtaaagatta tatttatgga
    33001 aagtaagcat attagtgttc ttatttgaac ttttatacca ctttttataa agctcatatt
    33061 catcaatctt tttctttaat aaatactggt cttctcctaa tactcttgtc atcaatctat
    33121 ctttcgcttc tttatagata ggatctccat atttataatc ttccaataag tctattgctc
    33181 cagtaaattg tgcctctaaa ctatcaatta ttttccaata gtaataatct gaaacagtag
    33241 ctcccttttt acttttctca ttcactcttg attctttact ataatccttt gttactatcg
    33301 catctgcttt gttatcccat gtaaataagc tagatgcaac tgctaatgcg cctagcgaaa
    33361 ttatttgctt tttcataatt ttttaattcc tccaaaatgt aattgcccaa tctacattaa
    33421 agaaacaaaa tattaaaaga cattaactat atattaacta gaataaacaa agcattaact
    33481 atctttttgt tatagttaat tagctttgca atatacaata gtgtataatt tttttgatta
    33541 atgtacatca aaggagtaac aaagcatgac aacacaaatg aaaatcaaaa catatttagt
    33601 tgctggtatt aaagcggcgc tccttgatac gactggtatt aaattagcaa gcaaatctga
    33661 aactacatca catacgtatc aacatcaagc gcttgtagat caattacatg aattaatagc
    33721 aaacactgac ttaaataaat tatcgtacct aaatttagat gcgtttcaaa aacgcgatat
    33781 tttagctgcg cactatattg caaaatccgc tatacgcact aaaaatttgg atcaaatgac
    33841 taaagcgaaa caaagattag aaagtattta caattcaatt tctaaccctt tgcattcaca
    33901 aaacaattaa taattcacca ataaatcatg taagtgtttg tgacgccaaa ttgccataca
    33961 aatacttgtc atatgaatat aaacgaatga aacgattgcc tatcccatag atggcaacat
    34021 taaaaagacc tctgaaggta tcatcttgat aactttagag gtctttgttt atattattca
    34081 aacaaaattc ttatagaacg atcgaagtat gtttcgtctt tcttttcttc tttaatctga
    34141 tcagctaatg ctgggtcatc tgtgataata ccatcaacat tggtttgtaa gtatttcgtt
    34201 aaatcttctt cgccgttaat agtccaagta tagacttctt tattttccaa gtgcgcttga
    34261 ttaacaagtc ttggcgaata agaaaaatct tcgatgacaa agaaatctaa tgatgtttct
    34321 ttaaaatgac caaactgcaa cggaatgata taaccacact tgagatatgg cgcttctttt
    34381 ttcaacttag tcatcacatc ataatccaaa gacatcacac gatattgatg ttcaacacca
    34441 tgctttttca aaatatcaat aacacgttgt gtataatctg ctggttcttt accatgtggc
    34501 tttaactcaa ctagtagctt cacatttgat tgtttagccg tttcaataaa ttcgtctaag
    34561 gatacaagtt ttgcttcatg tccattttga cgcattttca aaccgacgac atctttgaaa
    34621 ttagattcag aaatgttttt attaacacct gttaaacgtt tcaaattgtt atcatgacta
    34681 acaacaaatt gtttatcttt cgtcataatt gtatctaact caacgtattc gacattcgct
    34741 tttgcagcag ctttcaatga cggaatagaa ttttcaaccc ctttatcttc gaaaccacga
    34801 tggccaataa tggagatatt tgtattgata gtattattgt aaagtaagta catgttataa
    34861 ccgataaaac atgtcactgc aagcaccatt gaaattataa agaacctaga cttccgtttc
    34921 ggttttggat atttaaattc taagcccggt tggtctaata cattctcttg ttttaagtgc
    34981 agtactaaca cactgattaa tgataatttc gtaaataaat aatagaagaa caatgcgctt
    35041 ttcaatacaa caaataaaat tgatgagact aaaaacttat ctccttcttc atctacacaa
    35101 atagcaagat atgttgctcc tgaaataatt aatgttaaaa tcgcaccaat gatgagttcc
    35161 aatataacta tttctataac aagtcgaaac ttatttcgct tcgtaatttg ccaacttagt
    35221 ctcatatttt taaataacga ctggcggttt aaaatcgtta acggtaaggt aaatattaat
    35281 ttaaagttta atataaatac agcaatcata aaggtaccgt aaatgattat acctttcgtc
    35341 gttttcataa gttcttccgt taaaaattta ggtatgtaaa tattttttgt taatactgaa
    35401 cttagtccta ggttggcaat gggtatcatt aacattaaat aaatgacaaa gaaaataact
    35461 ggtacaccta tgagtttacg cacatttaca aaggcatttt taaaaatgga tttaaatgta
    35521 ataatctgtc gatcaaagcc ggcataaacc atataaacta acaatgaaaa ctctacataa
    35581 atcagaaagg caacacttaa tatgaatata ataagaagta tcacactggc aggatgactt
    35641 atgatttcca tccaattgtt aatcgtaagt tggctttgcc cagctacttt taacatcata
    35701 ttaaatagta aaattaagta tgtactacta ataaaaatca tgattaactg caatagtaag
    35761 gcattaatgc taaaacgccc tttattttga tacagtaatt taaatactgc ccatatatct
    35821 ttactaattc tcttcataat cacgctccgc attgctttaa tattaagttt catcttaata
    35881 tttttcatta ctcagggtca ataaaaattt gaaaagactc atattcatat gcaagtagca
    35941 attaataacc cattcgatga tattggaacc ccacctttaa caaggcatca cattctttat
    36001 cagcatagct acttacaaaa acgtattcct tcaaccattg taggacttgt ctcatatact
    36061 tactacgtct tgctaatact caatactcaa ttgaaaaaga agcatatgcc ccttcactct
    36121 tgaagttgca tatgctttct tttcggtctg aattgtattt ataattcaac gggaattttc
    36181 cctttgaagt taacataacg gtaggctgct ttaacagctt catcatcggg cgcttcgaca
    36241 tcttctaatt catatgcaat gcccaatgtt ttccacttat gaacacctaa ctgatgatat
    36301 ggcagaattt caaacttttc gacgttatca agagaattaa tgaattcccc tagtttaatt
    36361 aaatcgtctt tatcatcaga ataaccaggc acaaggacat gtcgaatcca tacaggttgt
    36421 ttcatatctg acagtttgcg cgcgaagtta aggatgtgtg tattaggctt tcctgtcaat
    36481 ctaatatgtt tgtcattatc aatatgtttt atatctaata atatcaagtc tgtatgtttt
    36541 tgtaattctt caaaatgcct ttgaaatgct tttgtatcat tagcacatcc agccgatgtg
    36601 tctaagcaag tgtgcacacc attttctttt aattccgcaa ataatttttc taagaatggc
    36661 atttgtaaca atggttcgcc accactgact gttacaccgc cacccgatgc atcaaagtat
    36721 ggtttgtatg gtaatatttc attcaccatt tcatcaactg tgacttctct tgatggctca
    36781 ctaattttcc aagtatctgg attgtggcaa tacaagcatc taagtaagca tccttgtgta
    36841 aataatatat atcttaatcc cggtccatcg acagtaccta aactttcgac agaatgtaag
    36901 tgtcccttaa gcatagtgct cccaccttaa attttgttac atactttcat ggaatgtacg
    36961 agaaattaca tctaattgtt gttcacgtgt taatttaatg aagttaacag cgtaaccaga
    37021 tacacggatt gttaactgtg gatattcttc tggatgttcc attgcatcta ttaatgtttc
    37081 acggttaaat acgttaatat ttaagtggtg accacattgc attgcgtaac catctaacat
    37141 actagttaag ttacggtttt gatcttctgg ttctttacct aatgattttg gtacgatact
    37201 gaatgtattt gaaataccat ctttacagca atcgtaaggg atcttagcta cagaacttaa
    37261 tgaagataat gcaccttttt ggtcacggcc atgcattggg tttgcacctg gagcaaatgg
    37321 ttcgccagct ttacgtccgt ctggtgtgtt accagttttc ttaccgtata caacgtttga
    37381 agtaattgtt aatacactca ttgtatgttc tgaatcacga tatgttttat gactacgtaa
    37441 tttagtcatg aagcgttcta ctaaatcaac tgcgatatca tctacacggt cgtcattgtt
    37501 accgtattta gggaagtcgc cttcgatttc aaagtctact acaagacctt cttcgttacg
    37561 aattggttta acttgtgcat atttaattgc agataatgag tcagctgcta ctgataaacc
    37621 agcgatacct gttgccattg tacgtacaat ttctgtatca tgtaatgcca tttcaatacg
    37681 ttcatagctg tatttatcgt gcatgtagtg aataacattt aatgagttaa tgtaaacacc
    37741 tgctagccaa tccatcattt gatcaaattt cttgaatact tcgtcatatt ctaatacttc
    37801 gctgttaata ccttcgaagt ttggaccaac ttgtgcacca gatttttcat ctttaccacc
    37861 attgattgcg taaagtaatg ttttagctaa gttcgcacgt gcaccgaaga attgcatttg
    37921 tttaccaatt gtcatcgctg atacacaaca tgcgatacca tagtcatcgc catagctttc
    37981 gcgcataatg tcatcatttt catattggat agaacttgtt ttaatactca tttttgcaca
    38041 gtatgttttg aagttgtcag gtaaacgtac tgaccataat actgttaagt ttggttctgg
    38101 agctggacct aagttatcta atgagtgtaa gaaacggaat gagtttttcg taacaagtgg
    38161 acgtccgtca atacctacac caccgataga ttcagttacc caagttgggt ctccagagaa
    38221 taattcattg taatcaggtg tacgagcaaa tttaacaata cgtaatttca tgatgaagtg
    38281 gtcaataatt tcttgaactt cgctttcagt aataacgcct gctttaaggt cacgttcagc
    38341 atagatatct aagaatgttg atgtacgacc taaactcatt gctgcaccgt tttgttcttt
    38401 aattgcagca aggtatgcta agtataacca ttgaactgct tctttgaagt tttctgctgg
    38461 acggcttaaa tcgaaaccat atttttgtcc aagttctttt aattctttta atgcacgata
    38521 ttgttctgat aattcttcac gtaaacgaat tacatcttct gacatttctg tagacatcgt
    38581 gttgaagtcg tgcatttttt cttccattaa gaaatctaca ccatataaag ctacacgacg
    38641 atagtcaccg ataatacgtc cacgtccgta tgcatcaggt aaaccagtga ttacacctgc
    38701 tttacggcag ttcaacattt ctctagaata tgcatcgaat acaccttggt tatgtgtttt
    38761 acgataatct gtaaagattt tttcagtttc ttcgtctaat tcgtaaccgt aagcttcaca
    38821 agctgctttc gccatacgaa taccaccgaa tggttgcatt gaacgtttga atggcttttc
    38881 agtttgtaca cctacaattg tttctaaatc tttgtctaaa taaccagcat catgagatgt
    38941 gattgttgaa gctactttcg tgtccatatc ccacatgccg ccacgttcac gttcttcttt
    39001 cgataactgc attacttgtt cccaaagttt agaagttgct tctgttggtc ctgctaaaaa
    39061 tgaatcatta ccttcataaa gagtgtagtt taattggata aactctctta catctacgtg
    39121 tctgttccat cttccatttt taaatccttg ccaagctgtt gcatgatttt tatttgtttc
    39181 taacatacta aacgcctcca tctgtataat gttgtgaatt tgaaatgcta tgttaacgtt
    39241 ttcatagtca ttgtagcatg tttgttgtga tttttttcac ataaattatg tacaattttc
    39301 atcaaatgta tgtcaaaaca tagaaactgt actaatttgt gtaacagtaa tatgctaatt
    39361 gcattctgtt acacaacagt tataatgttc actattttca ttgctttaaa agttaattta
    39421 ggagtatata tttgtttata aaaaatgatt gttgataagc gttaaatcaa cacttagaaa
    39481 gatttacttt tatatatgaa gagactggat taaatacttt tattgacgta aaaattcact
    39541 tttgaaccgt tcaatatctt gccgattttt atataacagc tacaaataaa atataacagt
    39601 ttgattttac agcctcggta aatcgtcttg acaaacaaaa attttgtgct atcacaacat
    39661 ttgcaacgtc ttaacaagtc atctataaac att
//
LOCUS       DAHHSC010000004        38411 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.004, whole genome shotgun sequence.
ACCESSION   DAHHSC010000004 DAHHSC010000000
VERSION     DAHHSC010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 38411)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38411)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38411
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.004"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            131..1468
                     /locus_tag="NBI57_000151"
     CDS             131..1468
                     /locus_tag="NBI57_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HCD1821149.1"
                     /translation="MKQPILNKLESLNQEEAISLHVPGHKNMTIGHLSQLSMTMDKTE
                     IPGLDDLHHPEEVILESMKQVEKHSDYDAYFLVNGTTSGILSVIQSFSQKKGDILMAR
                     NVHKSVLHALDISQQEGHFIETHQSPLTNHYNKVNLSRLNNDGHKLAVLTYPNYYGET
                     FNVEEVIKSLHQLNIPVLIDEAHGAHFGLQGFPDSTLNYQADYVVQSFHKTLPALTMG
                     SVLYIHKNAPYRETIIEYLSYFQTSSPSYLIMASLESAAEFYKTYDSTVFFDKRAQLI
                     ECLEKKGFEMLQVDDPLKLLIKYEGFTGHDIQNWFMNAHIYLELADDYQALAILPLWH
                     HDDTYLFDLLLRKIEDMILPKKSVSKVKQTQLLTTEGNYKPKRFEYVTWCDLKKAKGK
                     VLARHIVPYPPGIPIIFKGETITENMIELVNEYLETGMIVEGIKNNKILVEDE"
     gene            1470..2087
                     /locus_tag="NBI57_000152"
     CDS             1470..2087
                     /locus_tag="NBI57_000152"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HCD1821150.1"
                     /translation="MSAFITFEGPEGSGKTTVINEVYHRLVKDYDVIMTREPGGVPTG
                     EEIRKIVLEGNDMDIRTEAMLFAASRREHLVLKVIPALKEGKVVLCDRYIDSSLAYQG
                     YARGIGVEEVRALNEFAINGLYPDLTIYLNVSAEVGRERIIKNSRDQNRLDQEDLKFH
                     EKVIEGYQEIIHNESQRFKSVNADQPLENVVEDTYQTIIKYLEKI"
     gene            2115..2444
                     /locus_tag="NBI57_000153"
     CDS             2115..2444
                     /locus_tag="NBI57_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019467733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP receptor"
                     /protein_id="HCD1821151.1"
                     /translation="MKMIIAIVQDQDSQELADQLVKNNFRATKLATTGGFLRAGNTTF
                     LCGVNDDRVDEILSVINQTCGNREQLVSPITPMGGSADSYIPYPVEVEVGGATVFVMP
                     VDAFHQF"
     gene            2658..3584
                     /locus_tag="NBI57_000154"
     CDS             2658..3584
                     /locus_tag="NBI57_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HCD1821152.1"
                     /translation="MDEQQQLTNAYHSNKLSHAYLFEGDDAQTMKQVAINFAKLILCQ
                     TDSQCETKVSTYNHPDFMYISTTENAIKKEQVEQLVRHMNQLPIESTNKVYIIEDFEK
                     LTVQGENSILKFLEEPPDNTIAILLSTKPEQILDTIHSRCQHVYFKPIDKEKFINRLV
                     EQNMSKPVAEMISTYTTQIDNAIALNEEFDLLALRKSVIRWCELLLTNKPMALIGIID
                     LLKQAKNKKLQSLTIAAVNGFFEDIIHTKVNVEDKQIYSDLKNDIDQYAQKLSFNQLI
                     LMFDQLTEAHKKLNQNVNPTLVFEQIVIKGVS"
     gene            3585..4388
                     /locus_tag="NBI57_000155"
     CDS             3585..4388
                     /locus_tag="NBI57_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HCD1821153.1"
                     /translation="MPNVIGVQFQKAGKLEYYTPNDIQVDIEDWVVVESKRGIEIGIV
                     KNPLMDIAEEDVVLPLKNIIRIADDKDIDKFNCNERDAENALILCKDIVREQGLDMRL
                     VNCEYTLDKSKVIFNFTADDRIDFRKLVKILAQHLKTRIELRQIGVRDEAKLLGGIGP
                     CGRSLCCSTFLGDFEPVSIKMAKDQNLSLNPTKISGACGRLMCCLKYENDYYEEVRAQ
                     LPDIGEAIETPDGNGKVVALNILDISMQVKLEGHEQPLEYKLEEIETMH"
     gene            4405..4752
                     /gene="yabA"
                     /locus_tag="NBI57_000156"
     CDS             4405..4752
                     /gene="yabA"
                     /locus_tag="NBI57_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HCD1821154.1"
                     /translation="MDRNEIFEKIMRLEMNVNQLSKETSELKAHAVELVEENVALQLE
                     NDNLKKVLGNDEPTTIDTANSKPAKAVKKPLPSKDNLAILYGEGFHICKGELFGKHRH
                     GEDCLFCLEVLSD"
     gene            5026..5751
                     /locus_tag="NBI57_000157"
     CDS             5026..5751
                     /locus_tag="NBI57_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HCD1821155.1"
                     /translation="MLKENERFDQLIKEDFSIIQNDDVFSFSTDALLLGHFTKPRTKD
                     IVLDLCSGNGVIPLLLFAKHPRHIEGVEIQKTLVDMARRTFQFNDVDEYLTMHHMDLK
                     NVTKVFKPSQYTLVTCNPPYFKENQQHQHQKEAHKIARHEIMCTLEDCMIAARHLLKE
                     GGRLNMVHRADRLMDVLFEMRKVNIEPKKVVFIYSKVGKSAQTIVVEGRKGGNQGLEI
                     MPPFYIYNEDGNYSEEMKDVYYG"
     gene            5744..5992
                     /locus_tag="NBI57_000158"
     CDS             5744..5992
                     /locus_tag="NBI57_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HCD1821156.1"
                     /translation="MDSHFVYIVKCSDGSLYTGYAKDVNARVEKHNRGQGAKYTKVRR
                     PVHLVYQEMYETKSEALKREYEIKTYTRQKKLRLIKER"
     gene            5994..6833
                     /gene="rsmI"
                     /locus_tag="NBI57_000159"
     CDS             5994..6833
                     /gene="rsmI"
                     /locus_tag="NBI57_000159"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HCD1821157.1"
                     /translation="MAVLYLVGTPIGNLADITYRAVDVLKRVDMIACEDTRVTSKLCN
                     HYDIPTPLKSYHEHNKDKQTAFIIEQLELGLDVALVSDAGLPLISDPGYELVVAAREA
                     NIKVETVPGPNAGLTALMASGLPSYVYTFLGFLPRKEKEKSAVLEQRMHENSTLIIYE
                     SPHRVTDTLKTIAKIDATRQVSLGRELTKKFEQIVTDDVTQLQALIQQGDVPLKGEFV
                     ILIEGAKANNEISWFDDLSINEHVDHYIQTSQMKPKQAIKKVAEERQLKTNEVYNIYH
                     QIS"
     gene            7118..9091
                     /gene="metG"
                     /locus_tag="NBI57_000160"
     CDS             7118..9091
                     /gene="metG"
                     /locus_tag="NBI57_000160"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HCD1821158.1"
                     /translation="MAKETFYITTPIYYPSGNLHIGHAYSTVAGDVIARYKRMQGYDV
                     RYLTGTDEHGQKIQEKAQKAGKTEIEYLDEMIAGIKQLWAKLEISNDDFIRTTEERHK
                     HVVEQVFERLLKQGDIYLGEYEGWYSVPDETYYTESQLVDPQYENGKIIGGKSPDSGH
                     EVELVKEESYFFNISKYTDRLLEFYDQNPDFIQPPSRKNEMINNFIKPGLADLAVSRT
                     SFNWGVHVPSNPKHVVYVWIDALVNYISALGYLSDDESLFNKYWPADIHLMAKEIVRF
                     HSIIWPILLMALDLPLPKKVFAHGWILMKDGKMSKSKGNVVDPNILIDRYGLDATRYY
                     LMRELPFGSDGVFTPEAFVERTNFDLANDLGNLVNRTISMVNKYFDGELPAYQGPLHE
                     LDEEMEAMALETVKSYTESMESLQFSVALSTVWKFISRTNKYIDETTPWVLAKDDSQK
                     DMLGNVMAHLVENIRYAAVLLRPFLTHAPKEIFEQLNINNPQFMEFSSLEQYGVLTES
                     IMVTGQPKPIFPRLDSEAEIAYIKESMQPPATEEEKEEIPSKPQIDIKDFDKVEIKAA
                     TIIDAEHVKKSDKLLKIQVDLDSEQRQIVSGIAKFYTPDDIIGKKVAVVTNLKPAKLM
                     GQKSEGMILSAEKDGVLTLVSLPSAIPNGAVIK"
     gene            9122..9895
                     /locus_tag="NBI57_000161"
     CDS             9122..9895
                     /locus_tag="NBI57_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HCD1821159.1"
                     /translation="MLIDTHVHLNDEQYDDDLSEVITRAREAGVDRMFVVGFNKSTIE
                     RAMKLIDEYDFLYGIIGWHPVDAIDFTEEHLEWIESLAQHPKVIGIGEMGLDYHWDKS
                     PADVQKEVFRKQIALAKRLKLPIIIHNREATQDCIDILLEEHAEEVGGIMHSFSGSPE
                     IADIVTNKLNFYISLGGPVTFKNAKQPKEVAKHVSMERLLVETDAPYLSPHPYRGKRN
                     EPARVTLVAEQIAELKGLSYEEVCEQTTKNAEKLFNLNS"
     gene            10062..10598
                     /gene="rnmV"
                     /locus_tag="NBI57_000162"
     CDS             10062..10598
                     /gene="rnmV"
                     /locus_tag="NBI57_000162"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HCD1821160.1"
                     /translation="MKINEFIVVEGRDDTERVKRAVECDTIETNGSAINEQTLEVIRN
                     AQQSRGVIVLTDPDFPGDKIRSTITEHVKGVKHAYIDREKAKNKKGKIGVEHADLIDI
                     KEALMHVSSPFDEAYESIDKSVLIELGLIVGKDARRRREILSRKLRIGHSNGKQLLKK
                     LNAFGYTEADVRQALEDE"
     gene            10609..11502
                     /gene="rsmA"
                     /locus_tag="NBI57_000163"
     CDS             10609..11502
                     /gene="rsmA"
                     /locus_tag="NBI57_000163"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HCD1821161.1"
                     /translation="MLDNKDIATPSRTRALLDKYGFNFKKSLGQNFLIDVNIINNIID
                     ASDIDAQTGVIEIGPGMGSLTEQLARHAKRVLAFEIDQRLIPVLNDTLSPYDNVTVIN
                     EDILKANIKEAVENHLQDCEKIMVVANLPYYITTPILLNLMQQDIPIDGYVVMMQKEV
                     GERLNAEVGSKAYGSLSIVVQYYTETSKVLTVPKSVFMPPPNVDSIVVKLMQRTEPLV
                     TVDNEEAFFKLAKAAFAQRRKTINNNYQNYFKDGKQHKEVILQWLEQAGIDPRRRGET
                     LSIQDFAKLYEEKKKFPQLEN"
     gene            11602..11865
                     /locus_tag="NBI57_000164"
     CDS             11602..11865
                     /locus_tag="NBI57_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Veg family protein"
                     /protein_id="HCD1821162.1"
                     /translation="MPKSILDIKNSIDCHVGNRIVLKANGGRKKTIKRSGILKETYPS
                     VFIVELDQDKHNFERVSYTYTDVLTENVQVSFEEDNHHESIAH"
     gene            12175..13023
                     /locus_tag="NBI57_000165"
     CDS             12175..13023
                     /locus_tag="NBI57_000165"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HCD1821163.1"
                     /translation="MIYETAPAKINFTLDTLFKRNDGYHEIEMIMTTVDLNDRLTFHK
                     RKDRKIVVEIEHNYVPSNHKNLAYRAAQLFIEQYQLKQGVTISIDKEIPVSAGLAGGS
                     ADAAATLRGLNRLFDIGASLEELALLGSKIGTDIPFCIYNKTALCTGRGEKIEFLNKP
                     PSAWVILAKPNLGISSPDIFKLINLDKRYDVHTKMCYEALENRDYQQLCQSLSNRLEP
                     ISVSKHPQIDKLKNNMLKSGADGALMSGSGPTVYGLARKESQAKNIYNAVNGCCNEVY
                     LVRLLG"
     gene            13037..13861
                     /gene="purR"
                     /locus_tag="NBI57_000166"
     CDS             13037..13861
                     /gene="purR"
                     /locus_tag="NBI57_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HCD1821164.1"
                     /translation="MRYKRSERIVFMTQYLMNHPNKLIPLTFFVKKFKQAKSSISEDV
                     QIIKNTFQKEKLGTVITTAGASGGVTYKPMMSKEEATEVVNEVITLLEEKERLLPGGY
                     LFLSDLVGNPSLLNKVGKLIASIYMEEKLDAVVTIATKGISLANAVANILNLPVVVIR
                     KDNKVTEGSTVSINYVSGSSRKIETMVLSKRTLAENSNVLVVDDFMRAGGSINGVMNL
                     MNEFKAHVKGVSVLVESKEVKQRLIEDYTSLVKLSDVDEYNQEFNVEPGNSLSKFS"
     gene            13878..14258
                     /locus_tag="NBI57_000167"
     CDS             13878..14258
                     /locus_tag="NBI57_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HCD1821165.1"
                     /translation="MKIINTTRLPEALGPYSHATVVNGMVYTSGQIPLNVDGKIVSAD
                     VQAQTKQVLENLKVVLEEAGSDLNSVAKATIFIKDMNDFQKINEVYGQYFNEHKPARS
                     CVEVARLPKDVKVEIELVSKIKEL"
     gene            14330..14632
                     /gene="spoVG"
                     /locus_tag="NBI57_000168"
     CDS             14330..14632
                     /gene="spoVG"
                     /locus_tag="NBI57_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation regulator SpoVG"
                     /protein_id="HCD1821166.1"
                     /translation="MKVTDVRLRKIQTDGRMKALVSITLDEAFVIHDLRVIEGNSGLF
                     VAMPSKRTPDGEFRDIAHPINSDMRQEIQDAVMKVYDETDEVVPDKNATSEDSEEA"
     gene            14843..14974
                     /locus_tag="NBI57_000169"
     CDS             14843..14974
                     /locus_tag="NBI57_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1821167.1"
                     /translation="MRLNDARIFEVRKKLFLKLQRIKNNAFYMLKEYCRLNYNNDEV"
     gene            14997..16349
                     /gene="glmU"
                     /locus_tag="NBI57_000170"
     CDS             14997..16349
                     /gene="glmU"
                     /locus_tag="NBI57_000170"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HCD1821168.1"
                     /translation="MRRHAIILAAGKGTRMKSKKYKVLHEVAGKPMVEHVLESVKGSG
                     VDQVVTIVGHGAESVKGHLGERSLYSFQEKQLGTAHAVQMAKSHLEDKEGTTIVVCGD
                     TPLITKETLETLIAHHEDANAQATVLSASIQQPYGYGRIVRNASGRLERIVEEKDATQ
                     AEKDINEISSGIFAFNNKTLFEKLTQVKNDNAQGEYYLPDVLSLILNDGGIVEVYRTN
                     DVEEIMGVNDRVMLSQAEKAMQRRTNHYHMLNGVTIIDPDSTFIGPDVTIGSDTVIEP
                     GVRINGRTEIGEDVVIGQYSEINNSTIENGACIQQSVVNDASVGANTKVGPFAQLRPG
                     AQLGADVKVGNFVEIKKADLKDGAKVSHLSYIGDAVIGERTNIGCGTITVNYDGENKF
                     KTIVGKDSFVGCNVNLVAPVTIGDDVLVAAGSTITDDVPNDSLAVARARQTTKEGYRK
                     "
     gene            16496..17461
                     /locus_tag="NBI57_000171"
     CDS             16496..17461
                     /locus_tag="NBI57_000171"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HCD1821169.1"
                     /translation="MLNNEYKNSSLKIFSLKGNEALAQEVADQVGIELGKCSVKRFSD
                     GEIQINIEESIRGCDVFIIQPTSYPVNLHLMELLIMIDACKRASAATINIVVPYYGYA
                     RQDRKARSREPITAKLVANLIETAGATRMIALDLHAPQIQGFFDIPIDHLMGVPILAK
                     HFKDDPNINPEECVVVSPDHGGVTRARKLADILKTPIAIIDKRRPRPNVAEVMNIVGE
                     IEGRTAIIIDDIIDTAGTITLAAQALKDKGAKEVYACCTHPVLSGPAKERIENSAIKE
                     LIVTNSIHLDEDRKPSNTKELSVAGLIAQAIIRVYERESVSVLFD"
     gene            17611..18264
                     /locus_tag="NBI57_000172"
     CDS             17611..18264
                     /locus_tag="NBI57_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HCD1821170.1"
                     /translation="MASLKSIIRQGKQTRSDLKQLRKSGKVPAVVYGYGTKNVSVKVD
                     EVEFIKVIREVGRNGVIELGVGSKTIKVMVADYQFDPLKNQITHIDFLAINMSEERTV
                     EVPVQLVGEAVGAKEGGVVEQPLFNLEVTATPDNIPEAIEVDITELNINDSLTVADVK
                     VTGDFKIENDSAESVVTVVAPTEEPTEEEIEAMEGEQQTEEPEVVGESKEDEEKTEE"
     gene            18575..19147
                     /locus_tag="NBI57_000173"
     CDS             18575..19147
                     /locus_tag="NBI57_000173"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HCD1821171.1"
                     /translation="MKCIVGLGNIGKRFELTRHNIGFEVVDYILEKNNFSLDKQKFKG
                     AYTIERMNGDKVLFIEPMTMMNLSGEAVAPIMDYYNVNPEDLIVLYDDLDLEQGQVRL
                     RQKGSAGGHNGMKSIIKMLGTDQFKRIRIGVGRPTNGMTVPDYVLQRFSNDEMVTMEK
                     VIEHAARAIEKFVETSRFDHVMNEFNGEVK"
     gene            19147..22653
                     /gene="mfd"
                     /locus_tag="NBI57_000174"
     CDS             19147..22653
                     /gene="mfd"
                     /locus_tag="NBI57_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HCD1821172.1"
                     /translation="MTILTTLIKEDNHFQDLNQVFGQANTLVTGLSPSAKVTMIAEKY
                     AQSNQQLLLITNNLYQADKLETDLLQFIDAEELYKYPVQDIMTEEFSTQSPQLMSERI
                     RTLTALAQGKKGLFIVPLNGLKKWLTPVEMWQNHQMTLRVGEDIDVDQFLNKLVNMGY
                     KRESVVSHIGEFSLRGGIIDIFPLIGEPIRIELFDTEIDSIRDFDVETQRSKDNIEEV
                     DITTASDYIITEEVIRHLKEELKTAYENTRPKIDKSVRNDLKETYESFKLFESTYFDH
                     QILRRLVAFMYETPSTIIEYFQKDAIIAVDEFNRIKETEESLTVESDSFISNIIESGN
                     GFIGQSFIKYDDFETLIEGYPVTYFSLFATTMPIKLNHIIKFSCKPVQQFYGQYDIMR
                     SEFQRYVNQNYHIVVLVETETKVERMQAMLSEMHIPSITKLHRSMSSGQAVIIEGSLS
                     EGFELPDMGLVVITERELFKSKQKKQRKRTKAISNAEKIKSYQDLNVGDYIVHVHHGV
                     GRYLGVETLEVGQTHRDYIKLQYKGTDQLFVPVDQMDQVQKYVASEDKTPKLNKLGGS
                     EWKKTKAKVQQSVEDIAEELIDLYKEREMAEGYQYGEDTAEQTTFELDFPYELTPDQA
                     KSIDEIKDDMQKSRPMDRLLCGDVGYGKTEVAVRAAFKAVMEGKQVAFLVPTTILAQQ
                     HYETLIERMQDFPVEIQLMSRFRTPKEIKQTKEGLKTGFVDIVVGTHKLLSKDIQYKD
                     LGLLIVDEEQRFGVRHKERIKTLKHNVDVLTLTATPIPRTLHMSMLGVRDLSVIETPP
                     ENRFPVQTYVLEQNMSFIKEALERELSRDGQVFYLYNKVQSIYEKREQLQMLMPDANI
                     AVAHGQMTERDLEETMLSFINNEYDILVTTTIIETGVDVPNANTLIIEDADRFGLSQL
                     YQLRGRVGRSSRIGYAYFLHPANKVLTETAEDRLQAIKEFTELGSGFKIAMRDLNIRG
                     AGNLLGKQQHGFIDTVGFDLYSQMLEEAVNEKRGIKEPESEVPEVEVDLNLDAYLPTE
                     YIANEQAKIEIYKKLRKTETFDQIIDIKDELIDRFNDYPVEVARLLDIVEIKVHALHS
                     GITLIKDKGKIIDIHLSVKATENIDGEVLFKATQPLGRTMKVGVQNNAMTITLTKQNQ
                     WLDSLKFLVKCIEESMRISDEA"
     gene            22643..24169
                     /locus_tag="NBI57_000175"
     CDS             22643..24169
                     /locus_tag="NBI57_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HCD1821173.1"
                     /translation="MKHKEAFNGVVVLTAALIVIKILSAVYRIPYQNILGDTGLYAYQ
                     QVYPIVALGMILSMNAIPSAITQNIGKYHSDEAYAKAVAYIQLVGILLFIAIFVFANN
                     IAHMMGDSHLTPMIQAASLSFIFIGMLGVLRGYYQSANNMTVPAISQVIEQVIRVGII
                     IVTIVIFVDRGWTIYEAGTIAILASTIGFLGSSIYLVAHRPFKFKMVNNTAKIVWKQF
                     ALSVLIFAISQLIVILWQVIDSVTIIKSLQAIRVPFDVAITEKGVYDRGASFIQMGLI
                     VTTTFSFALIPLLSDAIKMNNQVLMNRYANASLKITILISTAAGIGLINLLPLMNGVF
                     FKTNDLTLTLSVYMITVICVSLIMMDMALLQAQHAVRPIFVGMTAGLVIKFILNIILI
                     RLSGIIGASISTVVSLIIFGTIIHIAVTRKYHLHAMRRFFINVVLGMVFMSIVVQCVL
                     NIVTTHGRFTGLIELLCAAVLGIIALFFYIFRFNVLTYKELTYLPFGSKLYQIKKGRR
                     "
     gene            24169..25362
                     /locus_tag="NBI57_000176"
     CDS             24169..25362
                     /locus_tag="NBI57_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HCD1821174.1"
                     /translation="MAHTITIVGLGNYGIDDLPLGIYKFLKTQDKVYARTLDHPVIES
                     LQDELTFQSFDHVYEAHDQFEDVYNDIVAQLVEAANEKDIVYAVPGHPRVAETTTVKL
                     LALAKDNTDIDVKVLGGKSFIDDVFEAVNVDPNDGFTLLDATSLQEVILNVRTHTLIT
                     QVYSAMVAANLKITLMERYPDDYPVQIVTGARSDGADNVVTCPLYELDHDENAFNNLT
                     SVFVPKIITSTYLYHDFDFATEVIDTLVDEDKGCPWDKVQTHETLKRYLLEETFELFE
                     AIDNEDDWHMIEELGDILLQVLLHTSIGKKEGYIDIKEVITSLNAKMIRRHPHIFGDA
                     NAETIDDLKEIWSKAKDAEGKQPRVKFEKVFAEHFLNLYEKTKDKSFDEASLKQWLEK
                     GESNT"
     gene            25359..25622
                     /locus_tag="NBI57_000177"
     CDS             25359..25622
                     /locus_tag="NBI57_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HCD1821175.1"
                     /translation="MRLDKYLKVSRLIKRRTLAKEVSDQGRITINGNVAKAGSDVKVE
                     DVLTIRFGQKLVTVKVTALNEHASKDNAKGMYEIIEERRLEEA"
     gene            25640..26032
                     /locus_tag="NBI57_000178"
     CDS             25640..26032
                     /locus_tag="NBI57_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HCD1821176.1"
                     /translation="MKNKVEHIENQYTSQENKKKQRQKMKMRVVRRRITVFAGVLLAI
                     IVVLSILLVVQKHRNDIDAQERKAKEAQFQKQQNEEIALKEKLNNLNDKDYIEKIARD
                     DYYLSNKGEVIFRLPEDKDSSSSKSSKK"
     gene            26137..26538
                     /locus_tag="NBI57_000179"
     CDS             26137..26538
                     /locus_tag="NBI57_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein S1"
                     /protein_id="HCD1821177.1"
                     /translation="MSIEVGNKLKGKVTGIKKFGAFVELPEGKSGLVHISEVADNYVE
                     NVEEHLSVGDEVDVKVLSIADDGKISLSIKKAKDRPRRQHTSKPSHQKPVQKAEDFEK
                     KLSNFLKDSEDKLTSIKRQTESRRGGKGSRR"
     gene            26718..28013
                     /gene="tilS"
                     /locus_tag="NBI57_000180"
     CDS             26718..28013
                     /gene="tilS"
                     /locus_tag="NBI57_000180"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HCD1821178.1"
                     /translation="MQLNSNGWHVDDHIVVAVSTGIDSMCLLYQLLNDYKDSYRKLTC
                     LHVNHGVRSASIEEARFLEAYCERHHIDLHIKKLDLSHSLDRNNSIQNEARIKRYEWF
                     DEMMNVLEADVLLTAHHLDDQLETIMYRIFNGKSTRNKLGFDELSKRKGYQIYRPLLA
                     VSKKEIKQFQERYHIPYFEDESNKDNKYIRNDIRNRIIPAIDENNQLKVSHLLKLKQW
                     HDEQYDILQYSAKQFIQEFVKFDEQSKYLEVSRQAFNNLPNSLKMVVLDCLLSKYYEL
                     FNISAKTYEEWFKQFSSKKAQFSINLTDKWIIQIAYGKLIIMAKNNGDTYFRVQTIKK
                     PGNYFFNKYRLEIHSNLPKCLFPLTVRTRQSGDTFKLNGRDGYKKVNRLFIDCKVPQW
                     VRDQMPIVLDKQQRIIAVGDLYQQQTIKKWIIISKNGDE"
     gene            28018..28557
                     /gene="hpt"
                     /locus_tag="NBI57_000181"
     CDS             28018..28557
                     /gene="hpt"
                     /locus_tag="NBI57_000181"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HCD1821179.1"
                     /translation="MHNDLKEVLLTEEDIQNICKELGAQLTKDYQGKPLVCVGILKGS
                     AMFMSDLIKRIDTHLSIDFMDVSSYHGGTESTGEVQIIKDLGSSIENKDVLIIEDILE
                     TGTTLKSITELLQSRKVNSLEIVTLLDKPNRRKADIEAKYVGKKIPDEFVVGYGLDYR
                     ELYRNLPYIGTLKPEVYSN"
     gene            28814..30907
                     /gene="ftsH"
                     /locus_tag="NBI57_000182"
     CDS             28814..30907
                     /gene="ftsH"
                     /locus_tag="NBI57_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HCD1821180.1"
                     /translation="MQKAFRNVLVIVIIGVIIFGLFSYLNGNGNMPKQLTYNQFTEKL
                     EKGDLKTLEIQPQQNVYMVSGKTKNGEDYSSTILYNNEKELQKITDAAKKQNGVKLTI
                     KEEEKQSVFVSILSTLIPVVVIALLFIFFLSQAQGGGSGGRMMNFGKSKAKMYDNNKR
                     RVRFSDVAGADEEKQELIEIVDFLKDNKKFKEMGSRIPKGVLLVGPPGTGKTLLARAV
                     AGEAGAPFFSISGSDFVEMFVGVGASRVRDLFDNAKKNAPCIIFIDEIDAVGRQRGAG
                     VGGGHDEREQTLNQLLVEMDGFGENEGIIMIAATNRPDILDPALLRPGRFDRQIQVGR
                     PDVKGREAILHVHAKNKPLDETVDLKAISQRTPGFSGADLENLLNEASLIAVREGKKK
                     IDMRDIEEATDRVIAGPAKKSRVISKKERNIVAHHEAGHTIIGMVLDEAEVVHKVTIV
                     PRGQAGGYAMMLPKQDRFLMTEQELLDKICGLLGGRVSEDINFNEVSTGASNDFERAT
                     QIARSMVTQYGMSKKLGPLQFGHSNGQVFLGKDMQGEPNYSSQIAYEIDKEVQRIVKE
                     QYERCKQILLEHKEQLILIAETLLTEETLVAEQIQSLFYEGKLPEIDYDAAKVVKDED
                     SEFNDGKFGKSYEEIRKEQLEDGQRDESEDRKEEKDIAEDKKEADKSDEKDEPAHRQA
                     PNIEKPYDPNHPDNK"
     gene            31135..32016
                     /gene="hslO"
                     /locus_tag="NBI57_000183"
     CDS             31135..32016
                     /gene="hslO"
                     /locus_tag="NBI57_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HCD1821181.1"
                     /translation="MTHDYIVKALAFDGEIRAYAALTTETVQEAQTRHYTWPTASAAM
                     GRTMTATAMMGAMLKGDQKLTVTVDGQGPIGRIIADANAKGEVRAYVDHPQTHFPLNE
                     QGKLDVRRAVGTNGSIIVVKDVGMKDYFSGASPIVSGELGEDFTYYYATSEQTPSSVG
                     LGVLVNPDNTIKAAGGFIIQVMPGAKDETISKLEKAISEMTPVSKLIEQGLTPEGLLN
                     EILGEDHVQILEKMPVQFECNCSHEKFLNAIKGLGEAEIQNMIKEDHGAEAVCHFCGN
                     KYKYTEEELNVLLESLA"
     gene            32195..33127
                     /gene="cysK"
                     /locus_tag="NBI57_000184"
     CDS             32195..33127
                     /gene="cysK"
                     /locus_tag="NBI57_000184"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HCD1821182.1"
                     /translation="MAQKPVDNITQIIGGTPVVKLRNVVDDNAADVYVKLEYQNPGGS
                     VKDRIALAMIEKAEREGKIKPGDTIVEPTSGNTGIGLAFVCAAKGYKAVFTMPETMSQ
                     ERRNLLKAYGAELVLTPGSEAMKGAIKKAKELKEEHGYFEPQQFENPANPEVHELTTG
                     PELLQQFEGKTIDAFLAGVGTGGTLSGVGKVLKKEYPNIEIVAIEPEASPVLSGGEPG
                     PHKLQGLGAGFIPGTLNTEIYDSIIKVGNDTAMEMSRRVAKEEGILAGISSGAAIYAA
                     IQKAKELGKGKTVVTVLPSNGERYLSTPLYSFDD"
     gene            33343..34146
                     /gene="folP"
                     /locus_tag="NBI57_000185"
     CDS             33343..34146
                     /gene="folP"
                     /locus_tag="NBI57_000185"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HCD1821183.1"
                     /translation="MTKTKIMGILNVTPDSFSDGGKFNNVETAINRVKAMIDEGADII
                     DVGGVSTRPGHEMVTLEEELNRVLPVVEAIVGFDVKISVDTFRSEVAEACLKLGVDMI
                     NDQWAGLYDHRMFQIVAKYDAEIILMHNGNGNRDEPVVEEMLTSLLAQAHQAKIAGIP
                     SNKIWLDPGIGFAKTRNEEAEVMARLDELVATEYPVLLATSRKRFTKEMMGYDTTPVE
                     RDEVTAATTAYGIMKGVRAVRVHNVELNAKLAKGIDFLKENENARHNLS"
     gene            34124..34489
                     /gene="folB"
                     /locus_tag="NBI57_000186"
     CDS             34124..34489
                     /gene="folB"
                     /locus_tag="NBI57_000186"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HCD1821184.1"
                     /translation="MQDTIFLKGMRFYGYHGALSAENEIGQIFKVDVTLKVDLAEAGR
                     TDNVIDTVHYGEVFEEVKSIMEGKAVNLLEHLAERIANRINSQYNRVMETKVRITKEN
                     PPIPGHYDGVGIEIVRENK"
     gene            34486..34962
                     /gene="folK"
                     /locus_tag="NBI57_000187"
     CDS             34486..34962
                     /gene="folK"
                     /locus_tag="NBI57_000187"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HCD1821185.1"
                     /translation="MIQAYLGLGSNIGDRESQLNDAIKILNEYDGISVSNISPIYETA
                     PVGYTEQPNFLNLCVEIQTTLTVLQLLECCLKTEECLHRIRKERWGPRTLDVDILLYG
                     EEMIDLPKLSVPHPRMNERAFVLIPLNDIAANVVEPRSKLKVKDLVFVDDSVKRYK"
     gene            35356..35448
                     /locus_tag="NBI57_000188"
     CDS             35356..35448
                     /locus_tag="NBI57_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1821186.1"
                     /translation="MVNDKVLETSKEMYVEQKCLIFYKTLKENV"
     gene            35501..36988
                     /gene="lysS"
                     /locus_tag="NBI57_000189"
     CDS             35501..36988
                     /gene="lysS"
                     /locus_tag="NBI57_000189"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HCD1821187.1"
                     /translation="MSEEMNDQMLVRRQKLQELYDLGIDPFGSKFDRSGLSSDLKEEW
                     DQYSKEELVEKEADSHVAIAGRLMTKRGKGKAGFAHVQDLAGQIQIYVRKDQVGDDEF
                     DLWKNADLGDIVGVEGVMFKTNTGELSVKAKKFTLLTKSLRPLPDKFHGLQDIEQRYR
                     QRYLDLITNEDSTRTFINRSKIIQEMRNYLNNKGFLEVETPMMHQIAGGAAARPFVTH
                     HNALDATLYMRIAIELHLKRLIVGGLEKVYEIGRVFRNEGVSTRHNPEFTMIELYEAY
                     ADYHDIMDLTESMVRHIANEVLGSAKVQYNGETIDLESAWTRLHIVDAVKEATGVDFY
                     EVKSDEEAKALAKEHGIEIKDTMKYGHILNEFFEQKVEETLIQPTFIYGHPTEISPLA
                     KKNPEDPRFTDRFELFIVGREHANAFTELNDPIDQKGRFEAQLVEKAQGNDEAHEMDE
                     DYIEALEYGMPPTGGLGIGIDRLVMLLTDSPSIRDVLLFPYMRQK"
     gene            37546..37660
                     /gene="rrf"
                     /locus_tag="NBI57_000190"
     rRNA            37546..37660
                     /gene="rrf"
                     /locus_tag="NBI57_000190"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            37673..37748
                     /locus_tag="NBI57_000191"
     tRNA            37673..37748
                     /locus_tag="NBI57_000191"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:37706..37708,aa:Val,seq:tac)
     gene            37765..37840
                     /locus_tag="NBI57_000192"
     tRNA            37765..37840
                     /locus_tag="NBI57_000192"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:37798..37800,aa:Thr,seq:tgt)
     gene            37847..37919
                     /locus_tag="NBI57_000193"
     tRNA            37847..37919
                     /locus_tag="NBI57_000193"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:37880..37882,aa:Lys,seq:ttt)
     gene            37953..38027
                     /locus_tag="NBI57_000194"
     tRNA            37953..38027
                     /locus_tag="NBI57_000194"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:37985..37987,aa:Gly,seq:gcc)
     gene            38035..38123
                     /locus_tag="NBI57_000195"
     tRNA            38035..38123
                     /locus_tag="NBI57_000195"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:38069..38071,aa:Leu,seq:taa)
     gene            38129..38205
                     /locus_tag="NBI57_000196"
     tRNA            38129..38205
                     /locus_tag="NBI57_000196"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:38163..38165,aa:Arg,seq:acg)
     gene            38226..38299
                     /locus_tag="NBI57_000197"
     tRNA            38226..38299
                     /locus_tag="NBI57_000197"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:38260..38262,aa:Pro,seq:tgg)
     gene            38323..38398
                     /locus_tag="NBI57_000198"
     tRNA            38323..38398
                     /locus_tag="NBI57_000198"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:38356..38358,aa:Ala,seq:tgc)
BASE COUNT        13761 a         5197 c         7778 g        11675 t
ORIGIN      
        1 attaattatt atacaataga caagctattg cataagtaac actaactttt gtcaaagaag
       61 tgttactata taattaatac ttttgaaagt aactaattcc aaaacagtgt aaataaagga
      121 agcgtatatc atgaagcaac ctattttaaa taaattagaa agtttaaatc aagaagaagc
      181 gatttctttg catgttccgg gtcataaaaa tatgactatc ggtcatttat ctcaattatc
      241 aatgacaatg gataaaactg aaatacctgg attagatgat ttacatcatc ctgaagaagt
      301 cattttggaa agtatgaagc aggtggagaa acattcagat tatgatgctt atttcttagt
      361 gaatggcacc acttcaggaa tattatctgt catccagtct ttttcacaga aaaaaggcga
      421 tatcttaatg gcaagaaatg tacataaatc tgtgttacat gcgctcgata ttagccaaca
      481 agaagggcat tttattgaaa cgcatcaaag tccgttaacg aatcattata ataaagttaa
      541 tttaagccgt ttgaataatg acggtcacaa acttgctgtg ttgacttatc ctaactatta
      601 cggtgaaaca tttaatgtag aagaggttat caaatctttg caccaattaa atattcctgt
      661 actcattgac gaagcacacg gcgcgcactt tggattgcaa ggatttccag attctacatt
      721 aaattatcaa gctgactatg ttgttcaatc ttttcataaa acgttaccag ctttaacgat
      781 gggctcggta ctttatattc ataaaaatgc accttataga gaaactatta tagaatatct
      841 aagctacttc caaacatcta gtccttcgta tttgattatg gctagtttag agtcagctgc
      901 cgagttctat aaaacatatg atagtaccgt gttttttgat aagagagcgc aattaatcga
      961 atgtttggag aagaagggtt ttgaaatgct tcaagttgat gatccgttga agttgctgat
     1021 aaaatatgaa ggttttacag gtcatgatat tcaaaattgg tttatgaatg cacatatcta
     1081 tttagaatta gcggacgact atcaagcatt agcgatattg ccgttatggc atcatgatga
     1141 tacgtattta tttgatttgc ttttacgtaa aattgaagat atgattttac cgaaaaaatc
     1201 agtttctaaa gttaaacaaa cacaactttt aacaactgaa ggtaactata aaccaaaacg
     1261 ctttgaatat gttacttggt gtgatttgaa aaaggcaaaa ggtaaagttc tggcgcgaca
     1321 tattgtcccg tatccgccag ggattcctat tattttcaaa ggagaaacaa taactgaaaa
     1381 tatgatagaa ttggtaaatg aatatctgga aactggaatg atagttgaag gaattaaaaa
     1441 taataaaatt ttagttgagg atgaataaaa tgtcagcttt tataactttt gagggcccag
     1501 aaggctctgg aaaaacaact gtaattaatg aagtttacca tagattagta aaagattatg
     1561 atgtcattat gactagagaa ccaggtggtg ttcctactgg tgaagaaata cgtaaaattg
     1621 tattagaagg caatgatatg gacattagaa ctgaagcaat gttatttgct gcatctagaa
     1681 gagaacatct tgtattaaag gtcataccag ctttaaaaga aggtaaggtt gtgttgtgtg
     1741 atcgctatat cgatagttca ttagcttatc aaggttatgc tagagggatt ggcgttgaag
     1801 aagtaagagc attaaacgaa tttgcaataa atggattata tccagacttg acgatttatt
     1861 taaatgttag tgctgaagta ggtcgcgaac gtattattaa aaattcaaga gatcaaaata
     1921 gattagatca agaagattta aagtttcacg aaaaagtaat tgaaggttac caagaaatca
     1981 ttcataatga atcacaacgg ttcaaaagcg ttaatgcaga tcaacctctt gaaaatgttg
     2041 ttgaagacac gtatcaaact atcatcaaat atttagaaaa gatatgatat aattgttaga
     2101 agaggtgtta taaaatgaaa atgattatag cgatcgtaca agatcaagat agtcaggaac
     2161 ttgcagatca acttgttaaa aataacttta gagcaacaaa attggcaaca acaggtgggt
     2221 ttttaagagc gggtaataca acattcttat gtggtgtcaa tgatgaccgt gtagatgaaa
     2281 tattgtctgt gattaatcaa acgtgtggta atagagaaca gttggtttca cctattacac
     2341 ctatgggagg cagtgcggat tcgtacattc catatccagt tgaagttgaa gttggcggtg
     2401 ctactgtatt tgttatgcca gttgatgcat tccatcaatt ttaattctat aatacaatca
     2461 tcaattagag atacttaaaa tagtgtatta ataagttatt aacaattttg ggttgcttga
     2521 ctgcgactag ttcagatgcc aatagatttg atttttgtgg ttctaaaaat aatcacaaat
     2581 catggtcgct attgttgcag taattagttg ctctttggca acctttttat ataaaagcaa
     2641 aagggagttt gtaatgaatg gatgaacagc aacaattgac gaatgcatat cattcaaata
     2701 aattatcgca tgcctattta tttgaaggtg atgatgcaca aacgatgaaa caagttgcga
     2761 ttaattttgc aaagcttatt ttatgtcaaa cagatagtca atgtgaaaca aaggttagta
     2821 catataatca tccagacttt atgtatatat caacaactga gaatgcaatt aagaaagaac
     2881 aagttgaaca acttgtgcgt catatgaatc aacttcctat agaaagcaca aataaagtgt
     2941 acatcattga agactttgaa aagttaactg ttcaagggga aaacagtatc ttgaaatttc
     3001 ttgaagaacc accggacaat acgattgcta ttttattgtc tacaaaacct gagcaaattt
     3061 tagacacaat ccattcaagg tgtcagcatg tgtatttcaa gcctattgat aaagaaaagt
     3121 ttataaatag attagttgaa caaaacatgt ctaagccagt agctgaaatg attagtactt
     3181 atactacgca aatagataat gcaatagctt taaatgaaga atttgattta ttagcattaa
     3241 ggaaatcagt tatacgttgg tgtgaattgt tgcttactaa caagccaatg gcacttatag
     3301 gtattattga tttattgaaa caggctaaaa ataaaaaact gcaatcttta actattgcag
     3361 ctgtgaatgg tttcttcgaa gatatcatac atacaaaggt aaatgtagag gataaacaaa
     3421 tatatagtga tttaaaaaat gatattgatc aatatgcgca aaagttgtcg tttaatcaat
     3481 taattttgat gtttgatcaa ctgacggaag cacataagaa attgaatcaa aatgtaaatc
     3541 caacgcttgt atttgaacaa atcgtaatta agggtgtgag ttagatgcca aatgtaatag
     3601 gtgttcagtt tcaaaaagcg ggaaaattag aatattatac acctaatgat atacaagtag
     3661 atatagaaga ctgggtagtt gtcgaatcta aaagaggcat agagataggt attgttaaaa
     3721 atccattaat ggatattgct gaagaggatg ttgtgttacc tcttaaaaat attattcgca
     3781 ttgctgatga caaagatatt gataaattta attgtaatga acgagatgct gaaaatgcat
     3841 taatactatg taaagacatt gtaagagaac aaggtttgga catgcgttta gtcaattgcg
     3901 aatatacatt agataaatcg aaagttattt ttaattttac ggcggatgat cgtattgatt
     3961 ttagaaaatt agtaaaaata ttagcgcaac atttaaaaac acgtatcgag ttgagacaaa
     4021 ttggtgtaag ggatgaagcc aaattgcttg gcggtatcgg accttgtggt aggtcgttat
     4081 gttgttctac atttttaggg gattttgaac cagtatcgat taagatggct aaggatcaaa
     4141 atttatcatt aaatccaact aaaatttctg gtgcatgtgg tcgtttgatg tgttgtttaa
     4201 aatatgaaaa tgactattat gaggaagtac gtgcacaatt acctgatatt ggtgaagcaa
     4261 ttgaaacgcc tgatggtaac gggaaagtag ttgctttaaa tatattagac atttctatgc
     4321 aggtgaagct tgagggacat gaacagccac ttgaatataa attagaagaa atagaaacta
     4381 tgcattaagg aggcattatt acatttggat cgcaatgaaa tatttgaaaa aataatgcgt
     4441 ttagaaatga atgtcaatca actttcaaag gaaacttcag aattaaaggc acatgcagtt
     4501 gaattagtag aagaaaatgt agcgcttcaa cttgaaaatg ataatttgaa aaaggtgttg
     4561 ggcaatgatg aaccaactac tattgatact gcgaattcaa aaccagcaaa agctgtgaaa
     4621 aagccattac caagtaaaga taatttggct atattgtatg gagaaggatt tcatatttgt
     4681 aaaggcgaat tatttggaaa acatcgacat ggtgaagatt gtctgttctg tttagaagtt
     4741 ttaagtgatt aatcaagcac actcaaatag tgttataatt ataaatgaat atggtttgga
     4801 taagtctgag acaatgcatg tttcaggctt taattgtgta taaagttttg gtgattgcat
     4861 aagagatggc ggtactaaat gttattatta agtgtgcacg cagtatcatt agttataaaa
     4921 tgtagctgtt aaaagtcaaa aatacatcga atgtagttag gcatataata taaaaagaga
     4981 tttcaattac tcaatagaaa aaggttgtct tcataggagt taaaaatgtt aaaagagaat
     5041 gaacgatttg atcaactaat caaagaagat tttagtatta ttcaaaatga tgatgttttt
     5101 tcgttttcaa cggatgcttt gttgttaggg cattttacaa aacctagaac aaaagatatt
     5161 gtgttggact tatgttcagg caatggggtg atacccttgt tattgtttgc gaaacatcca
     5221 cgacatatag aaggtgttga gattcaaaaa acacttgtcg atatggcgcg acgcacattt
     5281 caattcaatg atgttgatga atatttaaca atgcatcaca tggatttgaa aaacgttact
     5341 aaagtattta aaccttcaca atatacttta gtaacgtgta atccgcctta ttttaaagag
     5401 aatcagcaac accaacatca aaaagaagca cataagatag cgagacatga gattatgtgt
     5461 acacttgaag attgcatgat tgcagcccgt catttattaa aagaaggtgg caggctaaat
     5521 atggtacatc gtgcagatag actaatggat gtcttgtttg aaatgagaaa agtgaatatt
     5581 gaacctaaga aagtcgtttt tatatatagt aaggtaggga aatcagcaca aacgatagta
     5641 gtagaaggtc gaaaaggtgg aaatcaaggt ttagaaatca tgcccccatt ttatatttat
     5701 aatgaagatg gtaattatag cgaagaaatg aaggacgtat attatggata gtcattttgt
     5761 atatattgta aaatgtagtg atggaagttt atatacagga tacgctaaag acgttaatgc
     5821 acgtgttgaa aaacataacc gaggtcaagg agccaaatat acgaaagtaa gacgtccggt
     5881 gcatttagtt tatcaagaaa tgtatgagac aaagtctgaa gcattgaagc gtgaatatga
     5941 aattaaaact tataccagac aaaagaaatt gcgattaatt aaggagcgat agtatggctg
     6001 tattatattt agtgggcaca ccaattggta atttagcaga tattacttat agagcagttg
     6061 atgtattgaa acgtgttgat atgattgctt gtgaagacac tagagtaact agtaagctgt
     6121 gtaatcatta tgatattcca actccattaa agtcatatca cgaacataac aaggataagc
     6181 agactgcttt tatcattgaa cagttagaat taggtcttga cgttgcgctc gtatctgatg
     6241 ctggattgcc cttaattagt gatcctggat acgaattagt agtggcagcc agagaagcta
     6301 atattaaagt agagactgtg cctggaccta atgctgggct gacggctttg atggctagtg
     6361 gattaccttc atatgtatat acatttttag gatttttgcc acgaaaagag aaagaaaaaa
     6421 gtgctgtatt agagcaacgt atgcatgaaa atagcacatt aattatatac gaatcaccgc
     6481 atcgtgtgac agatacatta aaaacaattg caaagataga tgcaacacga caagtatcac
     6541 tagggcgtga attaactaag aagttcgaac aaattgtaac tgatgatgta acacaattac
     6601 aagcattgat tcagcaaggc gatgtaccat tgaaaggcga attcgttatc ttaattgaag
     6661 gtgctaaagc gaacaatgag atatcgtggt ttgatgattt atctatcaat gagcatgttg
     6721 atcattatat tcaaacttca cagatgaaac caaaacaagc tattaaaaaa gttgctgaag
     6781 aacgacaact taaaacgaat gaagtatata atatttatca tcaaataagt taatcacttt
     6841 atcgattata tgaaatttta aacgatttta taaacgcaag ctgtaatttt aaatggtaag
     6901 ttatcatttt gcattgatac tgataaaatg atgttgacta tgataaaaaa atgatgacat
     6961 cgacgttttt taatgtaaaa taaatacatt gaaagtaata aataccttaa cattgaataa
     7021 gatgaaaatg agatgacgag ataaatgttc gcgtccgttg aaatgcatag aaatcttaga
     7081 tattatttga agtgagacat tacgaggagg aacagttatg gctaaagaaa cattttatat
     7141 aacaacccca atatactatc ctagtgggaa tttacatata ggacatgcat attctacagt
     7201 ggctggagat gttattgcaa gatataagag aatgcaagga tatgatgttc gttatttgac
     7261 tggaacggat gaacacggtc aaaaaattca agaaaaagct caaaaagctg gtaagacaga
     7321 aattgaatat ttggatgaga tgattgctgg aattaaacaa ttgtgggcta agcttgaaat
     7381 ttcaaatgat gattttatca gaacaactga agaacgtcat aaacatgtcg ttgagcaagt
     7441 gtttgaacgt ttattaaagc aaggtgatat ctatttaggt gaatatgaag gttggtattc
     7501 tgttccggat gaaacatact atacagagtc acaattagta gacccacaat acgaaaacgg
     7561 taaaattatt ggtggcaaaa gtccagattc tggacacgaa gttgaactag ttaaagaaga
     7621 aagttatttc tttaatatta gtaaatatac agaccgttta ttagagttct atgaccaaaa
     7681 tccagatttt atacaaccac catcaagaaa aaatgaaatg attaacaact tcattaaacc
     7741 aggacttgct gatttagctg tttctcgtac atcatttaac tggggtgtcc atgttccgtc
     7801 taatccaaaa catgttgttt atgtttggat tgatgcgtta gttaactata tttcagcatt
     7861 aggctattta tcagatgatg agtcactatt taacaaatac tggccagcag atattcattt
     7921 aatggctaag gaaattgtgc gattccactc aattatttgg cctattttat tgatggcatt
     7981 agacttaccg ttacctaaaa aagtctttgc acatggttgg attttgatga aagatggaaa
     8041 aatgagtaaa tctaaaggta atgttgtaga ccctaatatt ttaattgatc gctatggttt
     8101 agatgctaca cgttattatc taatgcgtga attaccattt ggttcagatg gcgtatttac
     8161 acctgaagca tttgttgagc gtacaaattt cgatctagca aatgacttag gtaacttagt
     8221 aaaccgtacg atttctatgg ttaataagta ctttgatggc gaattaccag cgtatcaagg
     8281 tccacttcat gaattagatg aagaaatgga agctatggct ttagaaacag tgaaaagcta
     8341 cactgaaagc atggaaagtt tgcaattttc tgtggcatta tctacggtat ggaagtttat
     8401 aagtagaacg aataagtata ttgacgaaac aacgccttgg gtattagcta aggacgatag
     8461 ccaaaaagat atgttaggca atgtaatggc tcacttagtt gaaaatattc gttatgcagc
     8521 tgtattatta cgtccattct taacacatgc gccgaaagag atttttgaac aattgaacat
     8581 aaacaatcct caatttatgg aatttagtag tttagagcaa tatggtgtgc ttactgagtc
     8641 aattatggtt actgggcaac ctaaacctat tttcccaaga ttggatagcg aagcggaaat
     8701 tgcatatatc aaagaatcaa tgcaaccgcc tgctactgaa gaggaaaaag aagagattcc
     8761 tagcaaacct caaattgata ttaaagactt tgataaagtt gaaattaagg cagcaacgat
     8821 tattgatgct gaacatgtta agaagtcaga taagctttta aaaattcaag tagacttaga
     8881 ttctgaacaa agacaaattg tatcaggaat tgccaaattc tatacaccag atgatattat
     8941 tggtaaaaaa gtagcagttg ttactaacct gaaaccagct aaattaatgg gacaaaaatc
     9001 tgaaggtatg atattatctg ctgaaaaaga tggtgtatta accttagtaa gtttaccaag
     9061 tgcaattcca aatggtgcag tgattaaata actgtatttt taaaaattag gagagataat
     9121 tatgttaatc gatacacatg tccatttaaa tgatgagcaa tacgatgatg atttgagtga
     9181 agtgattaca cgtgctagag aagcaggtgt tgatcgtatg tttgtagttg gttttaacaa
     9241 atcgacaatt gaacgcgcga tgaaattaat cgatgagtat gattttttat atggcattat
     9301 cggttggcat ccagttgacg caattgattt tacagaagaa cacctggaat ggattgaatc
     9361 tttagctcag catccaaaag tgattggtat tggtgaaatg ggattagatt atcactggga
     9421 taaatctcct gcagatgttc aaaaggaagt ttttagaaag caaattgctt tagctaagcg
     9481 tttgaagtta ccaattatca ttcataaccg tgaagcaact caagactgta tcgatatctt
     9541 attggaggag catgctgaag aggtaggcgg gattatgcat agctttagtg gttctccaga
     9601 aattgcagat attgtaacta ataagctgaa tttttatatt tcattaggtg gacctgtgac
     9661 atttaaaaat gctaaacagc ctaaagaagt tgctaagcat gtgtcaatgg agcgtttgct
     9721 agttgaaacc gatgcaccgt atctctcgcc acatccgtat agagggaagc gaaatgaacc
     9781 ggcgagagta actttagtag ctgaacaaat tgctgaatta aaaggcttat cttatgaaga
     9841 agtgtgcgaa caaacaacta aaaatgcaga gaaattgttt aatttaaatt cataaagtta
     9901 aaagtgagaa agatcaccgc cataaatgta aacgatgcta tattcgttta atatgctatg
     9961 gttctttctc acttttttaa attaaaatat cgtacatgtg caatacgtac gatagagatg
    10021 gttagagctt tgaaattaag aattgtagga aggcgtttta aatgaaaatc aatgagttta
    10081 tagttgtaga aggacgagat gatactgagc gtgttaaacg agctgttgaa tgtgatacga
    10141 ttgaaacgaa tggtagtgcc atcaacgaac aaactttaga agtaattaga aatgctcaac
    10201 aaagtcgagg cgttattgta ttaacagatc cagatttccc aggagataaa attagaagta
    10261 caattactga acatgtcaaa ggtgttaaac atgcgtatat tgatagagaa aaagctaaaa
    10321 ataaaaaagg gaaaattggt gttgaacatg ccgacttaat tgatattaaa gaagcgttaa
    10381 tgcatgttag ttcacccttt gatgaagctt atgaatcaat tgataaatct gtgctaatag
    10441 agttggggtt aatcgttggg aaagatgcaa ggcgccgtag agaaatttta agtagaaaat
    10501 tgcgaatcgg ccattccaat ggtaagcagt tattgaaaaa gttaaatgca tttggttata
    10561 ccgaagcgga tgtaaggcaa gctttagaag atgaatgagg aagtgaaaat gttggataat
    10621 aaagatattg caacaccatc aagaacgcga gcgttgttag ataaatatgg ctttaatttt
    10681 aaaaaaagtt taggacagaa ctttttgata gatgtgaata tcattaataa tatcattgat
    10741 gcaagtgata ttgatgcaca aactggggtg attgaaattg gtccaggtat ggggtcgttg
    10801 acagaacaat tggccagaca tgctaaaaga gtattggcat ttgaaattga tcaacgttta
    10861 atacctgtat taaatgatac tctatcacct tatgataatg tgacggtgat taatgaagat
    10921 attttaaaag cgaatattaa agaagctgtt gaaaatcatt tacaagattg cgaaaaaata
    10981 atggttgttg caaacctgcc gtactatatt acgacgccaa ttttattaaa tttaatgcaa
    11041 caagatatac caattgatgg ctacgtggtg atgatgcaaa aagaagtggg cgaacgctta
    11101 aatgctgaag taggttcaaa agcatatggt tcattatcaa ttgtcgtaca atactataca
    11161 gagactagta aagtattaac ggtacctaaa tctgtattta tgccaccacc taatgttgat
    11221 tcaatagttg taaaactgat gcagagaact gaaccgttag taacagtaga taacgaggaa
    11281 gcattcttta agttagcaaa agcagcattt gcacaaagaa gaaagacaat taacaataac
    11341 tatcaaaatt attttaaaga tggtaaacaa cacaaagaag tgattttaca atggttggaa
    11401 caagcaggta ttgatccaag acgtcgcggt gaaacgctat ctattcaaga ttttgctaaa
    11461 ttgtatgaag aaaagaaaaa attccctcaa ttagaaaatt aaatgattga caaagcaaag
    11521 cactattgtt aaaatttaaa ttttgtttga cgaaaacgct gcaaatatgg tattatgtaa
    11581 cttgtagcga ggtggagcaa tatgccaaaa tcaattttgg acatcaaaaa ttctattgat
    11641 tgtcatgtag gaaatcgtat tgtactgaaa gccaatggag gccgtaagaa aacaataaaa
    11701 cgttctggaa ttttaaaaga aacatatccg tcagttttca ttgttgagtt agatcaagac
    11761 aaacacaact ttgagagagt atcttataca tacactgatg tgttaactga aaatgttcaa
    11821 gtttcatttg aagaggataa tcatcacgaa tcaattgcac actaaataag acatatagag
    11881 atgttaggcg tttcttagta taagaagtaa atattatgat aattatttga gtgttgggca
    11941 ttatgttcaa tactcttttt atttacaaaa tgtttaacac tgatgtttcg cttatagatt
    12001 tttcagtaaa tggataattg tatttataaa cacaaataca agtaaatact aagtaattag
    12061 atggagaaaa ttactttttt attaaaaaaa cactaaaaaa caaattaaaa tgtcaaatat
    12121 taattctctt tatgttaaaa tcatcatatt aagataacga aaagagggcg gaaaatgata
    12181 tatgaaacgg caccagccaa aattaatttt acgctcgata cactttttaa aagaaatgat
    12241 ggctatcatg agattgaaat gataatgaca acagttgatt taaatgatcg tttaactttt
    12301 cataaaagaa aagatcgaaa gatagttgtt gagattgaac ataattatgt gccttctaat
    12361 cataaaaatc tcgcatatcg tgcagcgcaa ctatttattg agcaatatca actaaagcaa
    12421 ggtgtaacaa tttctatcga taaagaaata cctgtttctg ctggcttagc tggaggttcg
    12481 gctgatgcag cagcaacgtt aagaggattg aatcgacttt ttgatatagg ggcgagtttg
    12541 gaagaattgg ctctactagg cagtaaaatt gggacagata ttccgttttg tatttataat
    12601 aaaactgcac tatgtactgg aagaggagag aaaatcgagt ttttaaataa accaccttca
    12661 gcttgggtga ttcttgctaa accaaactta ggcatatcat caccagatat atttaagttg
    12721 atcaatttag ataagcgtta cgacgtacat acgaaaatgt gttatgaggc cttagaaaat
    12781 cgagattatc aacaattatg tcaaagtttg tctaatcgat tagagccaat ttctgtttca
    12841 aaacacccac aaatcgataa attaaaaaat aatatgttga aaagtggtgc agatggtgcg
    12901 ttaatgagtg gaagcggacc gactgtgtat gggctagcac gaaaagaaag ccaagcaaaa
    12961 aatatttata atgcagttaa cggttgttgt aatgaagtgt acttagttag actattagga
    13021 tagaagggtt gaaaagatga gatataaacg aagcgagaga attgttttta tgacgcaata
    13081 tttgatgaac catccgaata aattgattcc attaactttt tttgtgaaaa aatttaaaca
    13141 ggcgaaatct tcaataagtg aagatgtcca aattataaaa aatacattcc aaaaagaaaa
    13201 gttaggtaca gtaattacta ctgctggcgc aagtggtggt gttacgtata aaccaatgat
    13261 gagtaaagaa gaggcgactg aagttgttaa tgaggtcatt actctattag aagagaaaga
    13321 acgtttgtta cctggcgggt atttattttt atcagatttg gtaggtaatc catcgctact
    13381 aaacaaagtt ggtaagttaa ttgccagtat ttacatggaa gaaaaattag atgctgttgt
    13441 taccattgcg acaaaaggta tttcattggc aaatgcggtt gctaatattt taaatttacc
    13501 agtagtagtg attagaaaag acaacaaggt gactgaaggt tctacagttt caattaatta
    13561 cgtttcagga tcttcaagaa aaatagagac aatggtactt tcgaagagaa ctttagcaga
    13621 aaattcaaat gttttagttg tcgatgattt tatgagggct ggtggctcta ttaatggtgt
    13681 tatgaattta atgaatgagt ttaaagccca tgtaaaaggg gtatcagtac ttgtagaatc
    13741 aaaagaagtt aaacaaagat tgattgaaga ttatacttcc ttagtgaaat tatctgatgt
    13801 agatgaatat aatcaagagt ttaacgtaga acctggcaac agtttatcta agttttcata
    13861 aaaggagttt tagtattatg aaaatcatta acacaacaag attaccggaa gcacttggac
    13921 catattcgca tgcaacagtt gtgaatggta tggtttatac ttctggtcag attccattga
    13981 atgttgatgg gaaaatcgta agcgctgatg ttcaagcaca gacaaaacaa gttttagaaa
    14041 atttaaaggt tgttttggaa gaagcaggat ctgatttgaa ttctgttgcg aaagcgacca
    14101 ttttcattaa agatatgaat gatttccaaa aaataaatga agtgtatggt caatatttta
    14161 atgaacacaa gccagcgcgt agttgtgtag aggttgcgcg tttgccaaaa gatgtgaaag
    14221 tagaaattga attagtaagt aaaattaagg aattataatt ttcgattaat atgtttaatc
    14281 aagcttctaa ataaaacaga gagatatata ctataggggg gctcactaca tgaaagtgac
    14341 agatgtaaga cttagaaaaa tacaaacaga tggacgaatg aaagcactcg tttccattac
    14401 attagatgaa gctttcgtaa ttcatgattt acgtgtaatt gaaggaaact ctggcttgtt
    14461 cgttgcaatg ccaagtaaac gtacaccaga tggtgaattc cgcgacatcg cgcatcctat
    14521 taattcagat atgagacaag aaattcaaga tgcagtgatg aaagtatatg atgaaacaga
    14581 tgaagtagta ccagataaaa acgctacatc agaagattca gaagaagctt aatcaatttt
    14641 atatttagcg atgtaataca tttgcaataa gttgatttga tactgtcgat aaagcataaa
    14701 gctttgtcgg cagttttttt agtttgtatt aatgtttttt tagtttgtat taatgttttt
    14761 ttatttttaa tgaaaggcta ataaatatat acgttaacag attatgatga tatgaaaatt
    14821 attgattcat tgctgataaa aattgcgttt gaatgatgct cgtatttttg aagtaagaaa
    14881 aaagttgttt ttaaaattac aacgaattaa aaacaatgcc ttttatatgt tgaaagagta
    14941 ttgcagatta aattataata atgacgaagt gtaaaattta atgggggtta atgttcatgc
    15001 gaagacacgc gataattttg gcagcaggta aaggcacaag aatgaaatct aaaaagtata
    15061 aagtgctaca cgaggttgct gggaaaccta tggtcgaaca tgtattggaa agtgtgaaag
    15121 gctctggtgt cgatcaagtt gtaaccatcg taggacatgg tgctgaaagt gtaaaaggac
    15181 atttaggcga gcgttcttta tacagttttc aagagaaaca actcggtact gcgcatgcag
    15241 tgcaaatggc gaaatcacac ttagaagaca aggaaggtac gacaatcgtt gtatgtggtg
    15301 acacaccgct catcacaaag gaaacattag aaacattgat tgcgcatcat gaggatgcta
    15361 atgctcaagc aactgtatta tctgcatcga ttcaacaacc atatggatac ggaagaatcg
    15421 ttcgaaatgc gtcaggtcgt ttagaacgca tagttgaaga gaaagatgca acgcaagctg
    15481 aaaaggatat taatgaaatt agttcaggta tttttgcgtt taataataaa acgttgtttg
    15541 aaaaattaac acaagtgaaa aatgataatg cgcaaggtga atattacctc cctgatgtat
    15601 tgtcgttaat tttaaatgat ggcggcatcg tagaagtgta tcgtaccaat gatgttgaag
    15661 aaatcatggg tgtaaatgat cgtgtaatgc ttagtcaggc tgagaaggcg atgcaacgtc
    15721 gtacgaatca ttatcacatg ctaaatggtg tgacaatcat cgatcctgac agcactttta
    15781 ttggtccaga cgttacaatt ggtagtgata cagtcattga accaggcgta cgaattaatg
    15841 gtcgtacaga aattggcgaa gatgttgtta ttggtcagta ctctgaaatt aacaatagta
    15901 cgattgaaaa tggtgcatgt attcaacagt ctgttgttaa tgatgctagc gtaggagcta
    15961 atactaaggt cggaccgttt gcgcaattga gaccaggcgc gcaattaggt gcagatgtta
    16021 aggttggaaa ttttgtagaa attaaaaaag cagatcttaa agatggtgcc aaggtttcac
    16081 atttaagtta tattggcgat gctgtaattg gcgaacgtac taatattggt tgcggaacga
    16141 ttacagttaa ctatgatggt gaaaataaat ttaaaactat cgtcggcaaa gattcatttg
    16201 taggttgcaa tgttaattta gtagcacctg taacaattgg tgatgatgta ttggtggcag
    16261 ctggttccac aatcacagat gacgtaccaa atgacagttt agctgtggca agagcaagac
    16321 aaacaacaaa agaaggatat aggaaataat catttacgta tttaaaatgg ctaggataaa
    16381 aggataatcc tatgtaatat taatgtaatc tttatgattt aatgattcgc atagtaatgg
    16441 agttacattt tatatataat agtaattgcg taagtaaata attggaggac tataaatgtt
    16501 aaataatgaa tataagaatt cgtcattaaa gattttttca ttgaaaggaa acgaagcatt
    16561 agcgcaagaa gttgctgacc aagtaggaat tgaactaggt aaatgttcag ttaaacgttt
    16621 tagtgatgga gaaattcaaa ttaatatcga agagagtatt cgtggttgtg acgtatttat
    16681 tattcaacca acatcatatc ctgtgaatct acatttaatg gaattattaa ttatgattga
    16741 tgcttgtaaa cgtgcttctg cagcaacaat caatattgta gtgccatatt atggatatgc
    16801 aagacaagat agaaaagccc gtagccgtga gccaatcact gctaaattag ttgcaaactt
    16861 aatcgaaaca gctggcgcaa ctcgtatgat tgcgttagac ttacatgcac cacaaattca
    16921 aggattcttt gatattccaa ttgaccactt aatgggtgtg ccaattcttg ctaaacattt
    16981 caaagatgat ccgaatatta acccagaaga atgtgtcgtt gtttcaccag accatggcgg
    17041 tgttacacgt gcacgtaaat tagctgacat tttaaaaact ccaattgcaa ttatagataa
    17101 acgtcgtcct agaccaaatg ttgctgaagt gatgaacatt gttggtgaga ttgaaggacg
    17161 tacggcaatt attattgacg atattattga tacagcaggt acaatcactt tagctgcaca
    17221 agcattaaaa gataaaggtg ctaaagaagt atatgcttgt tgtacacacc ctgttttatc
    17281 aggaccggct aaagaacgta tcgaaaattc tgctataaaa gaattaatcg taacaaactc
    17341 aattcattta gatgaagatc gcaaaccatc taacactaaa gaattatctg ttgctggttt
    17401 aatcgcacaa gctatcattc gtgtatacga aagagaatca gttagcgtat tatttgacta
    17461 atatttaaaa ggcgtttgac gaacatattc caaacgtgta taatagtttc gttcgtgatt
    17521 atacgaataa ataaacactt gcaagcaacg atgatgttga tgggtaagtg aggtgctcgt
    17581 tttgagcaaa aatgaaaggt ggaaatgaga atggcttcat taaagtcaat catccgtcaa
    17641 ggtaaacaaa cacgttcaga tcttaaacaa ttaagaaaat ctggtaaagt accagcagta
    17701 gtatacggtt acggtactaa aaacgtgtca gttaaagttg atgaagtaga attcatcaaa
    17761 gttatccgtg aagtaggtcg taacggtgtt atcgaattag gcgttggttc taaaactatc
    17821 aaagttatgg ttgcagacta ccaattcgat ccacttaaaa accaaattac tcacattgac
    17881 ttcttagcaa tcaatatgag tgaagaacgt actgttgaag taccagttca attagttggt
    17941 gaagcagtag gcgctaaaga aggcggcgta gttgaacaac cattattcaa cttagaagta
    18001 actgctactc cagacaatat tccagaagca atcgaagtag acattactga attaaacatt
    18061 aacgacagtt taactgttgc tgatgttaaa gtaactggcg acttcaaaat cgaaaacgat
    18121 tcagctgaat cagttgtaac agtagttgct ccaactgaag aaccaactga agaagaaatc
    18181 gaagctatgg aaggcgaaca acaaactgaa gaaccagaag ttgttggcga aagcaaagaa
    18241 gacgaagaaa aaactgaaga gtaattttaa tctgttacat taaagttttt atactttgtt
    18301 taacaagcac tgtgcttatt ttaatataag catggtgctt tttgtgttat tataaagctt
    18361 aattaaactt tattactttg tactaaagtt taattaattt tagtgagtaa aagatattaa
    18421 actcaacaat gatacatcat aaaaatttta atgtactcga ttttaaaata catacttact
    18481 aagctaaaga ataatgataa ttgatggcaa tggcggaaaa tagatgttgt cattataata
    18541 ataaatgaaa caattatgtt ggaggtaaac acgcatgaaa tgtattgtag gtctaggtaa
    18601 tataggtaaa cgttttgaac ttacaagaca taatatcggc tttgaagtcg ttgattatat
    18661 tttagagaaa aataattttt cattagataa acaaaagttt aaaggtgcat atacaattga
    18721 acgaatgaac ggagataaag tgttatttat cgaaccaatg acaatgatga atttgtcagg
    18781 agaagcagtt gcaccgatta tggattatta caatgttaat ccagaagatt taattgtctt
    18841 atatgatgat ttagatttag aacaaggaca agttcgctta agacaaaaag gaagtgcggg
    18901 cggtcacaat ggtatgaaat caattattaa aatgcttggt acagaccaat ttaaacgtat
    18961 tcgtattggt gtgggaagac caacgaatgg tatgacggta cctgattatg ttttacaacg
    19021 cttttcaaat gatgaaatgg taacgatgga aaaagttatc gaacacgcag cacgcgcaat
    19081 tgaaaagttt gttgaaacat cacgatttga ccatgttatg aatgaattta atggtgaagt
    19141 gaaataatga caatattgac aacgcttata aaagaagata atcattttca agaccttaat
    19201 caggtatttg gacaagcaaa cacactagta actggtcttt ccccgtcagc taaagtgacg
    19261 atgattgctg aaaaatatgc acaaagtaat caacagttat tattaattac caataattta
    19321 taccaagcag ataaattaga aacagattta cttcaattta tagatgctga agaattgtat
    19381 aagtatcctg tgcaagatat tatgaccgaa gagttttcaa cacaaagccc tcaactgatg
    19441 agtgaacgta ttagaacttt aactgcgtta gctcaaggta agaaagggtt atttatcgtt
    19501 cctttaaatg gtttgaaaaa gtggttaact cctgttgaaa tgtggcaaaa tcaccaaatg
    19561 acattgcgtg ttggtgagga tatcgatgtg gaccaatttc ttaacaaatt agttaatatg
    19621 gggtacaaac gggaatccgt ggtatcgcat attggtgaat tctcattgcg aggaggtatt
    19681 atcgatatct ttccgctaat tggggaacca atcagaattg agctatttga taccgaaatt
    19741 gattctattc gcgattttga tgttgaaacg cagcgttcca aagataatat tgaagaagtc
    19801 gatatcacaa ctgcaagtga ttatatcatt actgaagaag tgattagaca tcttaaagaa
    19861 gagttgaaaa ctgcatatga aaatacaaga cccaaaatag ataaatcagt gcgcaatgat
    19921 ctgaaagaaa cgtatgaaag ctttaaatta ttcgaaagta catactttga tcatcaaata
    19981 ctacgtcgtt tagtagcgtt tatgtatgaa acaccttcga caattattga gtatttccaa
    20041 aaagatgcaa tcattgcagt tgatgaattt aatcgtatta aagaaactga agaaagttta
    20101 acagtagagt ctgattcgtt tattagcaat attattgaaa gtggtaatgg atttatagga
    20161 caaagtttta taaaatatga tgattttgaa acattgattg aaggctatcc tgtcacttat
    20221 ttttcattat tcgctacaac aatgccgata aaactaaatc atattattaa attttcatgt
    20281 aaacctgtcc aacaatttta tgggcaatat gacattatgc gttctgaatt tcaacgatat
    20341 gtcaatcaaa attatcatat cgtggttttg gtcgaaactg aaactaaagt tgaacgtatg
    20401 caagcgatgt taagtgaaat gcatattcca tcaataacaa aattgcatcg ctcaatgtca
    20461 tcggggcaag cagtgattat tgaaggcagt ttatctgaag gatttgaact acctgatatg
    20521 ggattagttg tcattactga gcgtgagctt tttaaatcaa aacagaaaaa gcaacgaaaa
    20581 cgtacgaaag ctatctcaaa tgctgaaaaa attaagtctt accaagattt aaatgtggga
    20641 gattatattg ttcatgtgca tcatggtgtt ggtagatatt taggtgttga gacgctcgaa
    20701 gtggggcaaa cgcatcgtga ttatattaaa ttgcaatata aaggtacgga tcaactattt
    20761 gttccagtag atcaaatgga tcaagttcaa aaatatgtag cttcggaaga taagacgcca
    20821 aaattaaata aactcggtgg cagtgaatgg aaaaaaacaa aagcaaaagt tcaacaaagt
    20881 gttgaagata ttgctgaaga gttgattgat ttatataaag aaagagaaat ggcagaaggt
    20941 tatcaatatg gggaagacac agctgagcaa acaacatttg aattagattt tccatatgaa
    21001 cttacgcctg accaagctaa atctatcgat gaaattaaag atgacatgca aaaatcgcgt
    21061 ccaatggatc gcttgctatg tggtgatgtt ggttatggta aaactgaagt tgcagtgaga
    21121 gcagcattca aagctgtaat ggaaggaaag caagttgcat ttttagttcc tacaactatt
    21181 ttagctcagc aacattatga gacgttaatt gagcgtatgc aagattttcc tgttgaaatt
    21241 caattaatga gtcgttttag aacgccgaaa gagataaaac aaactaagga aggacttaaa
    21301 actgggtttg ttgacatagt tgttggtaca cacaaattac ttagtaaaga tatacagtat
    21361 aaagatttag ggctgttgat tgtagatgaa gaacaacgat ttggtgtacg ccataaagag
    21421 cgtattaaaa cattaaaaca taatgtagat gtactaacat tgactgcaac cccaatacct
    21481 agaacattgc atatgagtat gctaggtgtg cgcgatttgt cagtgattga aacgccgcca
    21541 gaaaatcgtt tcccagttca aacatatgta ttagaacaga acatgagttt tatcaaagaa
    21601 gctttagaaa gagaactatc ccgtgatggc caagtgtttt atctttataa taaagtgcaa
    21661 tccatttatg aaaaacgaga acaactccag atgttaatgc cagatgctaa cattgcagtt
    21721 gctcatggac aaatgacaga gcgcgattta gaagaaacga tgttaagttt tatcaataat
    21781 gaatatgata ttttagtaac gacgacgatt attgaaacag gtgtcgatgt cccaaatgca
    21841 aatactttga tcattgaaga tgcagatcgc tttggattga gtcagttgta tcaattaaga
    21901 ggtcgtgttg gtcgttcaag tcgtattggt tatgcatact tcttacatcc agcaaataag
    21961 gtactaactg agactgcaga agatcgatta caagcgatta aagaatttac ggagttaggc
    22021 tcaggattta agattgcgat gcgtgatttg aacattcgtg gtgctggtaa tttgttaggt
    22081 aaacaacagc acggctttat tgatacagtt ggatttgatt tgtacagtca aatgttagaa
    22141 gaagctgtaa atgaaaaacg tggtattaag gaaccagaat ctgaggtgcc agaagtcgaa
    22201 gttgatttaa acttggatgc atatttgcca acagaatata ttgcaaatga acaagctaaa
    22261 attgaaattt ataaaaagct acgaaaaact gaaacatttg atcaaattat cgacattaaa
    22321 gatgaattaa ttgatcgttt taatgattat cctgttgaag tagcacgttt gcttgatata
    22381 gtggaaataa aagtacacgc attacattca ggtatcacgt tgattaaaga taaagggaaa
    22441 ataattgata ttcatttatc tgtaaaagcc actgaaaata ttgatggcga agtgctgttc
    22501 aaagcaacac aacctttagg tagaacaatg aaggttggtg ttcaaaataa tgcaatgaca
    22561 attactttaa cgaaacaaaa tcaatggctt gatagtttga agtttttagt taagtgcatt
    22621 gaagaaagca tgagaatcag tgatgaagca taaagaagca tttaatggcg ttgtcgtgtt
    22681 aactgctgca ttaattgtca ttaaaattct gagtgctgta tatcgaattc catatcaaaa
    22741 tatattaggt gatacaggtt tgtatgcata tcaacaagtg tatccaattg tagcattagg
    22801 aatgatatta tcgatgaatg ccattcctag tgcaattaca caaaatatag ggaagtatca
    22861 tagtgacgaa gcatatgcaa aagcggtcgc ttatatacaa ttagttggta tattattatt
    22921 tattgctatt tttgtgtttg cgaacaatat tgcacatatg atgggtgata gccatttaac
    22981 accaatgatt caagcagcaa gtttaagctt tatatttata ggtatgcttg gcgtgttaag
    23041 aggttattat caatctgcaa ataatatgac agttccggct atttcccagg ttatagaaca
    23101 agttatacga gtaggtatta tcattgttac tattgttatt tttgtagaca gaggttggac
    23161 gatatatgaa gcgggaacaa ttgctatttt agcatcaacg ataggttttt taggttcttc
    23221 aatttattta gtagcgcacc gaccttttaa gtttaaaatg gtaaataaca ctgcaaagat
    23281 tgtttggaaa cagttcgcac tttcggtttt gattttcgct atcagtcaat taatcgtaat
    23341 tttatggcaa gtgattgata gtgttactat tattaagtca cttcaagcga tacgcgtgcc
    23401 attcgatgtt gccataactg aaaaaggagt ctatgaccgt ggtgcatcat ttattcagat
    23461 gggattgatt gtaactacaa catttagttt tgcgctcatt cctctgttaa gtgacgcaat
    23521 caaaatgaat aatcaggtac ttatgaatcg ttatgcaaat gcgtcattaa agattacgat
    23581 tttaataagt acagcagcgg gaataggatt aattaattta ttgcctttaa tgaacggtgt
    23641 gttttttaag acgaatgatt taaccttaac gttaagtgtt tatatgatta cggtcatttg
    23701 tgtatcgtta attatgatgg atatggcttt attacaagcg caacatgctg tgagacctat
    23761 ttttgttggt atgacggcag gattggttat taaatttata cttaatatca ttttgattcg
    23821 tttaagtggc attattggtg cgagcattag tactgttgta tcattaatta tattcggtac
    23881 gattatccat attgctgtca cgagaaaata ccacttacat gcgatgagac gattttttat
    23941 caatgttgtt ttaggtatgg tatttatgtc gattgttgtt caatgcgtgt taaacatagt
    24001 gacaacacac ggtagattca ctggactcat tgaattatta tgtgcagcag tattaggtat
    24061 cattgcattg tttttctata tttttagatt taatgttttg acatataaag agttaactta
    24121 tttaccattt ggttcaaagt tgtatcaaat taagaaagga agacgttgat ggcacatacc
    24181 attacgattg ttggcttagg aaactatggc attgatgatt tgccgctagg gatatataaa
    24241 tttttaaaga cacaagataa agtttatgca agaacgttag atcatccagt tatagaatca
    24301 ttgcaagatg aattaacatt tcagagtttt gaccatgttt atgaagcaca tgaccaattt
    24361 gaagatgtct ataacgatat tgtggcgcaa ttggttgaag ctgctaatga aaaagatatt
    24421 gtctatgcgg ttccgggtca tcctagagtt gctgagacaa ctacagtgaa attactggct
    24481 ttagcaaagg acaatactga tatagatgtg aaagttttag gtggtaaaag ctttattgat
    24541 gatgtgtttg aagcagttaa tgtagatcca aatgatggct tcacactgtt agatgcgaca
    24601 tcattacaag aagtaatact taatgttaga acgcatacat tgattacgca agtttatagt
    24661 gcaatggttg ctgctaattt gaaaatcact ttaatggaac gatatcctga tgattaccct
    24721 gttcaaattg tcactggtgc acgaagcgat ggtgcggata acgttgtgac atgcccatta
    24781 tatgaattgg atcatgatga aaatgcattc aataatttga cgagtgtatt cgtaccaaaa
    24841 atcataacat cgacatattt gtatcatgac tttgattttg caacggaagt gattgatact
    24901 ttagttgatg aagataaagg ttgtccatgg gataaagtgc aaacgcatga aacgcttaag
    24961 cgttatttac ttgaagaaac atttgaattg ttcgaagcta ttgacaatga agatgattgg
    25021 catatgattg aagaactagg agatatttta ttacaagtgt tattgcatac tagtattggt
    25081 aaaaaagaag ggtatatcga cattaaagaa gtgattacaa gtcttaatgc taaaatgatt
    25141 cgtagacacc cacacatatt tggtgatgcc aatgctgaaa ctatcgatga cttaaaagaa
    25201 atttggtcta aggcgaaaga tgctgaaggt aaacagccaa gagttaaatt tgaaaaagta
    25261 tttgcagagc attttttaaa tttatatgag aagacgaagg ataagtcatt tgatgaggcc
    25321 tcgttaaagc agtggctaga aaaaggggag agtaatacat gagattagat aaatatttaa
    25381 aagtatcacg gttaataaag cgacgtacgc tagcaaaaga agtaagtgat caaggtagaa
    25441 ttacaataaa tggtaatgtt gctaaagctg gatcggatgt taaagttgaa gatgtgctga
    25501 cgattcgctt tggtcaaaaa ttagtaacag ttaaagtaac tgcattaaat gaacatgcat
    25561 ctaaagataa cgcgaagggc atgtatgaaa tcattgaaga gcgtcgactt gaagaagcgt
    25621 aaattggagg tgacaagcaa tgaaaaataa agtagaacat atagaaaatc agtacacgtc
    25681 gcaagagaac aagaaaaaac aacgtcaaaa aatgaaaatg cgtgttgttc gtaggcgtat
    25741 tacagtattt gcgggcgtat tacttgcgat aattgttgtt ttatcaatct tgcttgttgt
    25801 ccaaaaacat cgcaatgata ttgatgcaca ggagcgaaaa gcgaaagaag cacagtttca
    25861 aaagcaacaa aatgaagaaa ttgcgttaaa agaaaagttg aataatctga atgacaaaga
    25921 ttacattgaa aaaattgcgc gtgatgatta ttacttaagc aacaaaggtg aagtgatttt
    25981 taggttgcca gaagacaaag attcgtctag ctcaaaatct tcgaaaaaat aaatccaaat
    26041 tgattcaaaa ttatccgagt atagacattg tgaaaaaatc caaacaagga tataataagg
    26101 gaaaatcgaa tcaaatcggg aggatttatt taacatatgt caatcgaagt tggaaataag
    26161 cttaaaggta aagtcactgg tattaaaaag tttggtgcat tcgtagaatt acctgaagga
    26221 aaaagtggtt tagttcacat tagtgaagtc gcagataatt atgttgaaaa cgtagaagag
    26281 cacctttctg ttggtgatga agtagacgtg aaagtattat ctattgctga tgatggaaaa
    26341 attagtcttt caattaagaa agctaaagac cgtccacgta gacaacatac gagtaaacca
    26401 agtcatcaaa aaccagtgca aaaagccgaa gattttgaaa agaaattaag caatttctta
    26461 aaagatagtg aagataaatt aacttcaatc aaacgtcaaa cagaatctag acgcggtggc
    26521 aaaggttcta gacgttaatt aaaataaata aagactgttt cgataaggaa tatatttaga
    26581 atgatgcgta tcgaataatc gattgcagcg ttagacaatc taagactgtt tcttaaataa
    26641 ggagcagtct cttttatttg taatgatata actaagactt ataccatttt tgaaaattgt
    26701 aaaagtgagg tgatgttatg cagttaaata gtaatggttg gcatgttgat gaccatattg
    26761 ttgtcgctgt ttctacaggt attgatagta tgtgtttatt gtatcaacta ctaaatgatt
    26821 ataaagatag ttatagaaaa ctaacatgct tacatgtcaa tcatggcgtt aggtcagctt
    26881 caattgagga agccagattt ttagaagcat actgcgaacg tcatcacatt gatttacata
    26941 tcaaaaagtt agatttgtcg catagtctcg accgaaataa cagcattcag aatgaagctc
    27001 gaattaaacg ttacgaatgg tttgatgaaa tgatgaatgt attagaagcg gatgtattgc
    27061 taacggcgca tcatttggac gatcaattag aaactattat gtatcgtatt tttaatggga
    27121 aatcaacgcg taataaacta ggatttgatg agttatcgaa gcgaaaaggt tatcagattt
    27181 atcgaccact tttagctgtc tctaaaaaag aaataaaaca attccaagag agatatcata
    27241 ttccatattt tgaagatgaa tctaataaag ataacaaata tattagaaat gatattcgta
    27301 atagaattat tccagctatt gatgaaaata atcaacttaa agtatcgcat ttattaaaat
    27361 taaaacaatg gcatgatgaa caatatgata ttttgcaata ttcagctaaa caatttattc
    27421 aagaatttgt gaagtttgat gaacagtcaa aatatttaga ggtttctaga caagctttta
    27481 ataacttacc aaactcatta aagatggttg tgttggactg cctattatca aagtattatg
    27541 agttgtttaa tattagtgct aaaacatacg aagagtggtt taaacaattt agtagtaaga
    27601 aagcacaatt cagtattaat ctcacggata aatggataat tcaaatcgca tatggtaaat
    27661 taataataat ggctaaaaat aatggcgata catattttag agttcaaaca attaaaaagc
    27721 caggtaatta tttttttaac aaatatcgat tagagataca ttctaattta ccaaaatgtt
    27781 tatttccgct tacagtgaga acacgacaaa gtggcgatac atttaaactg aatgggcgcg
    27841 atggttataa gaaagtgaat cgcctgttta tagattgtaa agtgccacag tgggttcggg
    27901 atcaaatgcc aatcgtattg gataaacaac agcgcattat tgcggtagga gatttatatc
    27961 aacaacaaac aataaaaaaa tggattataa ttagtaaaaa tggagatgaa tagcgttatg
    28021 cataatgatt tgaaagaagt attgttaact gaagaagata ttcaaaatat ctgtaaggaa
    28081 ttgggagcac aattaacaaa ggattatcaa ggtaaaccat tagtatgcgt gggtatctta
    28141 aaaggctcag caatgtttat gtcagattta attaaacgaa ttgataccca tttatcaatt
    28201 gatttcatgg atgtttctag ttatcacgga ggcactgagt caactggtga agttcaaatc
    28261 attaaagatt taggttcttc tattgaaaat aaagacgtat taattattga agatatctta
    28321 gagactggta ctacacttaa gtcaattact gaattattac aatctagaaa agttaattca
    28381 ttagaaatag ttactttatt agataaacca aaccgtcgta aagcggacat tgaagctaag
    28441 tatgtaggta aaaaaatacc agatgaattt gttgttggtt acggtttaga ttatcgtgaa
    28501 ttataccgaa acttaccata tatcggtacg ttaaaacctg aagtgtattc aaattaattt
    28561 tttaatcaat ttcagttatt attactatgc gtttgagaaa taatagtgta gactcaaaaa
    28621 tatgaaaaat gtatttcata tatatttaat tttagacaag acatatgtct tgaaaagttg
    28681 aaaaatatag agattgataa aactaatacg ggtgtgaatg acattgatgt taagctcaat
    28741 tactagctta taaaacatgt catatgttac aatttttgtt agttttatta tgggaagtag
    28801 gaggaaatga cgcatgcaga aagcttttcg caatgtgcta gttatcgtaa taataggcgt
    28861 tattattttt ggtctatttt catatttaaa cggtaatgga aatatgccga aacagcttac
    28921 atataatcaa tttactgaga agttggaaaa aggtgacctt aaaactttag aaatccaacc
    28981 acaacaaaat gtctatatgg taagtggtaa aacgaaaaat ggtgaagact attcatcaac
    29041 tattttatat aacaacgaaa aagaattaca aaaaattact gatgctgcta aaaagcaaaa
    29101 cggtgtaaaa ttaacgatta aagaagaaga aaaacaaagt gtctttgtga gtatactttc
    29161 aacattaatt ccagttgtag tcatagcgtt attatttatt ttcttcctaa gccaagcaca
    29221 aggtggcggt agtggcggtc gtatgatgaa ctttggtaaa tctaaagcaa aaatgtacga
    29281 taataataaa cgtcgtgttc gtttctctga tgtagcaggg gcagatgaag aaaaacaaga
    29341 attaattgaa attgttgatt tcttgaaaga taataaaaaa ttcaaagaaa tgggatctag
    29401 gattcctaaa ggtgtcttac ttgttggacc tccaggtact ggtaaaacat tacttgctag
    29461 agcggttgca ggtgaagctg gcgcaccatt cttctctatt agtggttcag actttgtaga
    29521 gatgtttgtt ggtgttggtg cgagccgtgt tcgtgactta ttcgataatg ctaagaaaaa
    29581 cgcgccttgt atcatcttta tcgatgagat tgatgctgtt ggtcgtcaac gtggtgcagg
    29641 tgttggtggc ggtcatgatg aacgtgaaca aaccctaaac caattattag ttgaaatgga
    29701 tggtttcggt gaaaatgaag gtatcattat gatagctgct acaaaccgtc ctgatatcct
    29761 tgacccagcc ttattacgtc caggtcgttt tgatagacaa attcaagttg gtcgtccaga
    29821 tgtgaaaggc cgtgaagcaa ttcttcatgt tcatgctaaa aacaaaccac ttgatgaaac
    29881 ggttgattta aaagcaattt cacaacgtac acctggtttc tcaggtgctg atttagagaa
    29941 cttattaaat gaagcatctt taattgctgt acgtgaaggt aaaaagaaaa ttgacatgag
    30001 agatatcgaa gaggcaacgg atagagttat agccggacct gctaagaaat ctcgagttat
    30061 ttctaagaaa gaacgtaata ttgttgctca tcacgaagct ggtcatacaa ttatcggtat
    30121 ggtacttgat gaggcagaag tagtgcataa agttactatt gttccacgtg gacaagcagg
    30181 tggttatgca atgatgctac ctaaacaaga tcgtttctta atgactgaac aagagttatt
    30241 agataaaatc tgtggtttac ttggtggacg tgtatcagaa gatattaact ttaacgaagt
    30301 atcaacaggt gcttcaaatg acttcgaacg tgcaacacaa atcgcacgct caatggttac
    30361 gcaatatggt atgagtaaaa aattaggacc attacagttc ggtcatagca atggtcaagt
    30421 attcttaggt aaagatatgc aaggtgagcc taattattca agccaaatcg catatgaaat
    30481 tgataaagaa gttcaacgaa tcgttaaaga acaatacgaa cgttgtaaac aaattttatt
    30541 agagcacaaa gaacaattaa ttttaattgc tgaaacatta ttaacagaag aaacattagt
    30601 tgctgaacaa attcaatcat tattctacga aggtaaatta cctgaaattg attatgatgc
    30661 agctaaagtt gttaaagatg aagattctga atttaatgat ggtaaattcg gtaaatctta
    30721 tgaagagatt cgtaaagagc aattagaaga tggacaacgt gacgaaagtg aagatcgtaa
    30781 agaagaaaaa gatattgctg aggataaaaa agaagctgat aaatctgatg aaaaagatga
    30841 accagcacat cgacaagccc caaatatcga aaaaccttac gatccaaatc acccagacaa
    30901 taaataatcg attatattca gtacctcttt ctatgataaa gttatagaaa gaggtacttt
    30961 tatcgttttt aaaaatacgt attagatttt aagtcattga attgttatag cagaaaataa
    31021 ttgtaaaaca agttacttca ttatttagaa tgatgggtgt agaataagta caattgttgc
    31081 attttatgaa gtaaagtaat tttaaaatat agagtaatag aggagattga aataatgaca
    31141 cacgattata ttgttaaagc attagcattt gatggggaga ttagggctta tgctgctttg
    31201 acaactgaaa ctgttcaaga agcacaaacg agacattata catggccgac agcatctgct
    31261 gcaatgggaa gaacaatgac agcaacagct atgatgggcg caatgttgaa aggtgatcaa
    31321 aaattaactg tcactgtaga tggccaagga cctattggac gaattattgc cgatgcaaat
    31381 gctaaaggcg aggtgcgtgc ttatgtagac catccacaaa ctcattttcc attaaatgag
    31441 caaggtaaac ttgatgtaag acgagcagta gggacaaatg gatctattat agttgttaaa
    31501 gacgttggaa tgaaagacta tttctctgga gcaagtccaa ttgtttcagg agaacttggt
    31561 gaagatttta cttattatta tgctacaagt gaacaaacac cttcatcggt aggtcttggt
    31621 gtattggtaa atcctgataa tacgattaaa gcagcaggag gatttatcat tcaagttatg
    31681 ccaggtgcca aagatgaaac aatttcaaaa ttagaaaaag caattagtga aatgacacca
    31741 gtttctaaat taattgaaca aggattaacg ccagaaggat tactaaacga aatcttaggt
    31801 gaagaccatg tgcaaatttt agagaaaatg cctgttcaat ttgaatgtaa ttgtagtcat
    31861 gagaaatttt taaatgctat taaaggattg ggcgaggctg agattcaaaa tatgattaaa
    31921 gaagatcatg gtgctgaagc agtatgtcat ttctgtggaa ataaatataa atatactgaa
    31981 gaagaattaa acgtgttgct agaaagttta gcgtaattta atttaaatca atacgctaaa
    32041 atgtttattt ttagcggttt agtgaaatgt agaactaaat agttgtataa tccttagtga
    32101 ttttgtttgc tttctagaat ttatttgata aaataattct atatccgata aataaactaa
    32161 gatttcaaca actaactaaa aaggagtgtt cttaatggca caaaaaccag tagataatat
    32221 tactcaaatt attggcggta caccggtagt caaattgaga aatgtagtag atgacaatgc
    32281 agcagatgtt tatgtaaaat tggaatatca aaatccaggt ggttctgtaa aggatagaat
    32341 tgctttagca atgattgaaa aagcagagcg agaaggcaaa attaaacctg gcgacacaat
    32401 tgtagaacca acaagtggta atacaggtat cggtttagca tttgtatgtg ctgctaaagg
    32461 atataaagca gtatttacta tgcccgaaac aatgagccaa gagcgtcgta atttattaaa
    32521 agcatacggt gcggaattag ttttaacgcc tggatcagaa gcgatgaaag gtgcaattaa
    32581 aaaagctaaa gaattaaaag aagaacatgg ttacttcgag ccacaacaat ttgaaaaccc
    32641 tgcgaaccct gaagttcatg agttaactac aggtcctgag ttattacaac aatttgaagg
    32701 gaaaactatc gatgcgttcc tagctggtgt tggtactggt ggtacgttat ctggtgtagg
    32761 taaagttctg aaaaaagaat atcctaacat cgaaattgtt gctatagagc ctgaggcttc
    32821 tccagtattg agcggtggtg agccaggtcc acataaatta caaggtttag gtgctggatt
    32881 tattccaggc actttgaata cagaaatcta tgacagtatt attaaagtag gaaatgatac
    32941 agcgatggaa atgtctcgtc gagttgctaa agaggaaggt attttagcag gtatttcatc
    33001 aggtgctgcg atttatgctg ccattcaaaa agcaaaagaa ttaggaaaag gtaaaacagt
    33061 agtaacagta ttgccgagta atggtgaacg ctacttatca acacctttat attcattcga
    33121 tgactaatta atgtcattta aaagagtgag ttatcttttt gagataactt gctctttttt
    33181 tctaccatgt ataattttaa aaatatgagc gttaaattaa acatttttct gataaaaaca
    33241 tccagtgaat gataagataa taaacgtaca tattaataac tagtaaatcg caggagtaaa
    33301 ttttattaga gttaaacaat acataattaa agggtggtta acatgactaa aacaaaaatt
    33361 atgggcatat taaacgtcac acctgattca ttctcagatg gtggaaaatt taataatgtt
    33421 gaaacagcta taaatagagt gaaagccatg atagatgaag gtgctgacat tatagatgtt
    33481 ggaggtgttt caacgagacc cggtcatgaa atggttacat tagaagaaga gctgaacaga
    33541 gtattacctg ttgttgaagc tattgtcggt tttgatgtaa aaatttcagt cgatacattt
    33601 cgaagtgagg ttgctgaagc atgtttaaaa ttaggcgttg atatgattaa tgatcaatgg
    33661 gcgggtctgt atgatcatcg tatgttccaa attgtagcta aatatgacgc ggaaattatt
    33721 ttaatgcata atggaaatgg taatcgtgat gaaccggttg tcgaagaaat gttaacatct
    33781 ttgttagcac aagcacatca agctaaaata gctggtatac cttcaaataa aatttggcta
    33841 gatccaggta taggtttcgc taaaactaga aatgaagaag ccgaagttat ggcaagactg
    33901 gatgaacttg ttgcaacaga atatccagtt ttattagcga caagccggaa acgtttcact
    33961 aaagagatga tgggttatga tacaacaccg gttgaaagag atgaagtaac tgcagctacg
    34021 actgcatatg gtattatgaa aggcgttaga gcagtacgcg ttcataatgt cgagttgaat
    34081 gctaaattag ctaaaggtat agatttttta aaggagaatg aaaatgcaag acacaatctt
    34141 tcttaaaggt atgcgctttt atggatatca tggtgcttta tcagctgaaa atgaaatagg
    34201 gcaaattttc aaagtggatg taactttgaa agtagactta gctgaagctg ggcgtactga
    34261 taatgttatt gatacagttc attatggtga agtgttcgaa gaggttaaat caattatgga
    34321 aggtaaggcc gttaatttac ttgagcatct agctgaacgt attgcaaatc gtataaattc
    34381 acaatataat cgtgtaatgg aaacaaaagt gagaatcact aaagaaaacc caccgattcc
    34441 gggtcattat gatggagtag gtatcgaaat agtgagggag aataaatgat tcaagcatac
    34501 ttaggattag gtagtaatat tggtgataga gaaagccagt taaacgatgc tataaagatt
    34561 ttgaatgaat atgatggtat tagcgtatct aatatttctc cgatttatga aacagcacca
    34621 gttgggtata ctgagcaacc taacttttta aatttgtgtg ttgaaattca aacaacactc
    34681 acagtattac aactgttgga atgttgtttg aagacagaag aatgtttaca ccgtattaga
    34741 aaggaacgat ggggtcctag aactttagat gtggatattt tgttgtatgg agaagaaatg
    34801 atagatttac caaaactgtc ggtgccacat ccgagaatga atgaacgtgc atttgtttta
    34861 atcccattaa atgatatagc agcaaatgtc gtagaaccac gttcgaaatt gaaagtgaaa
    34921 gatttagttt ttgtcgatga cagtgtaaag agatataaat aatgtattgt tgagaacatt
    34981 catatttatt gggaatagat gattgattat tggagatgtt tccgcatttt gattatctct
    35041 aaatgcattt gattacgaat tagtatatga tgatttagta tgtgttagaa ttaagttatt
    35101 tcataaaatc aaagtaagca atatttgata aattgctgta atttatttgg gcttttgttg
    35161 aagttcgtgg aagataattt ttatacattt acttagggtt gtattaatga taatcgtatt
    35221 ttaaatgaat aagtaattca taaacgaaaa cagaaattgc tttattacat ttacaagctc
    35281 ttgtaaaatt aaaaaagtga taaaattgat gttatgaata taagaaaaca tgccacatca
    35341 taactttaag gtgtaatggt taatgataaa gtattagaaa catcgaaaga gatgtatgtt
    35401 gagcaaaaat gtctgatatt ttataaaact ttaaaggaaa atgtttgagt gtaccagttg
    35461 gaatactaaa ggattacaac aagttaaagg agagaaagtt atgtcagaag aaatgaatga
    35521 ccaaatgttg gttcgacgtc aaaaattaca agaattatat gatcttggta tagacccgtt
    35581 tggttctaaa tttgaccgtt caggtttatc tagtgatttg aaagaagagt gggaccagta
    35641 ttctaaagaa gaattggtag aaaaagaagc ggatagtcat gtcgctatag ctggacgatt
    35701 aatgactaag cgtggtaaag gtaaagcagg atttgcacac gttcaggact tagctggaca
    35761 aattcaaatt tacgttcgta aagatcaagt tggcgatgac gaatttgatt tatggaaaaa
    35821 tgctgattta ggcgatatcg ttggtgttga aggtgtaatg ttcaaaacaa atactggcga
    35881 attatcggtt aaagcgaaga aattcacgct actaactaaa tcattgcgac cattaccgga
    35941 taaattccac ggtttacagg atattgaaca gagatatcgt caaagatatt tagatttaat
    36001 tacgaacgaa gatagcactc gtacatttat taatcgtagt aaaatcattc aagaaatgcg
    36061 taattattta aataataaag gtttcttgga agtagaaaca cctatgatgc accaaattgc
    36121 tggtggcgca gctgctagac catttgtaac acatcataat gcattagatg caacgttata
    36181 catgcgtatt gctattgagt tgcatttaaa acgtttaatt gtcggtggac ttgaaaaagt
    36241 atatgaaatt ggtagagtat tccgtaatga aggtgtatca actagacata accctgaatt
    36301 cacaatgatt gaattatatg aagcatatgc agattatcat gacattatgg atttaacaga
    36361 atctatggtg agacatattg ccaatgaagt attaggttct gcaaaagtac aatacaatgg
    36421 ggaaacgatt gatttagaat ctgcttggac tcgtttgcat attgttgatg ctgtaaaaga
    36481 agctactggt gtggattttt atgaagttaa aagtgatgaa gaagctaaag ctttagctaa
    36541 agaacatggt attgaaatta aagatacaat gaaatatggt catattttaa atgaattctt
    36601 tgagcaaaaa gttgaagaaa cacttattca gccaacgttt atctatggtc atccgactga
    36661 aatttcacct ttagcgaaga aaaatcctga agatcctaga tttactgatc gtttcgaatt
    36721 gttcattgta ggtagagagc atgcaaatgc atttactgaa ttaaatgatc ctattgatca
    36781 aaaaggtcgt tttgaagcgc aacttgttga aaaggcgcaa ggtaatgatg aagcgcatga
    36841 aatggatgaa gattacattg aagcgttaga atatggtatg cctccgacag gtggtcttgg
    36901 tatcggtatt gacagattgg ttatgttatt aactgattct ccatcaatca gagacgtgtt
    36961 attattccct tatatgagac aaaaataaat gacgttgatt gttagtaaga gctctcgtgt
    37021 atacaacatg tgtatgcgag ggttttctta attatggtaa ttagttcgtg tttgaatgtt
    37081 tttgatagta atagttaacg atagtggtac tatttttgac tgttaaacaa ggtagttggc
    37141 tgatagatga aaatgacgat acgtatatag agtagttcgg atgagaaatg ttaacgatag
    37201 acattgagat atcttataac aaaaattgct atattagtat aatttatctt aatcagctat
    37261 aaaagtactt taaaattgta tagaatgtgt atggttttgt acatatgtgt atgatagaat
    37321 actaaaagtg ttatgaatta gagagcacga gaaatgtcag ttttgaagaa taaaaaagtt
    37381 gattaaaagt gttgacttta tcaattgaat gaagtaatat ataaaagtcg tcaaaaacag
    37441 acgaaacaca ctaaaagctg atgtgacaaa gttcacaaca aagtgtaaaa ttaactattg
    37501 caccttatta attaagcgtg tatcataaat aagtaagtta ttttgtctgg tggctatagc
    37561 aaggaggtca cacctgttcc catgccgaac acagaagtta agctccttag cgtcgatggt
    37621 agtcgaactt acgttccgct agagtagaac gttgccaggc aaatgacaaa tcggagaatt
    37681 agctcagctg ggagagcatc tgccttacaa gcagagggtc ggcggttcga acccgtcatt
    37741 ctccaccatt tattcttaga tatagccggc ctagctcaat tggtagagca actgacttgt
    37801 aatcagtagg ttgggggttc aagtcctctg gccggcacca tcttttgagc cattagctca
    37861 gctggtagag catctgactt ttaatcagag ggtcagaggt tcgaatcctc tatggctcat
    37921 tacgatttaa tttttatatt tagcaaaata atgcagaagt agttcagcgg tagaatacaa
    37981 ccttgccaag gttggggtcg cgggttcgaa tcccgtcttc tgctccatta ttttgccggg
    38041 gtggcggaac tggcagacgc acaggactta aaatcctgcg gtgagagatc accgtaccgg
    38101 ttcgattccg gtcctcggca ccattttagc gcccgtagct caattggata gagcgtttga
    38161 ctacggatca agaggttatg ggttcgactc ctatcgggcg cgccattttt aaattaattg
    38221 aataacggga agtagctcag cttggtagag cacttggttt gggaccaagg ggtcgcaggt
    38281 tcgaatcctg tcttcccgat tacttcttaa atttcatttt atgggggctt agctcagctg
    38341 ggagagcgcc tgctttgcac gcaggaggtc agcggttcga tcccgctagt ctccaccatt
    38401 tatttttaca c
//
LOCUS       DAHHSC010000005        38108 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.005, whole genome shotgun sequence.
ACCESSION   DAHHSC010000005 DAHHSC010000000
VERSION     DAHHSC010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 38108)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38108)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38108
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.005"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            complement(<1..516)
                     /locus_tag="NBI57_000199"
     CDS             complement(<1..516)
                     /locus_tag="NBI57_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HCD1821188.1"
                     /translation="MKKYDIAVLDFETMNEHMNSPCEVAVSLIKDLSIVKVYSSYINP
                     PNNRYNLKNAKIHKIPEDVILKAPKYPDIYQEILYLLKESHLIIAHNALFDISVLKNT
                     NNYYDLPVPNFMYVDSINIFRSFHAISSFKLENLCSLYDIDKEKLHSAKFDVLALSKM
                     LISLAKNNQHYS"
     gene            complement(532..1245)
                     /locus_tag="NBI57_000200"
     CDS             complement(532..1245)
                     /locus_tag="NBI57_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCD1821189.1"
                     /translation="MNKERNIIIAKNIRKFLNDSNMSQKKLAELINIKPSTLSDYLNL
                     RSNPSHGVIQRIADVFEVGKSDIDTTYKDDNDITSIYNKLTPPRQENVLNYANEQLEE
                     QNSKGDNVVDINSYKQEKTPVNVNGCVSAGVGERLHDETLFTEMVKGPIPTHDLALKV
                     NGDSMEPMFKDGEIIFVEKTHNIKNGQIGIFIIEEEAYVKKVFVEDDRLTLVSLNKDY
                     DDLHFYRNESVRLIGKVIL"
     gene            1208..1382
                     /locus_tag="NBI57_000201"
                     /pseudo
     CDS             1208..1382
                     /locus_tag="NBI57_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500090.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
     gene            1379..1642
                     /locus_tag="NBI57_000202"
     CDS             1379..1642
                     /locus_tag="NBI57_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCD1821190.1"
                     /translation="MKTLKELRTDYGLTQKELGDLFKVSSRTIQNMEKDSTNIKDSLL
                     SKYMSAFNVKYDDIFLGNEYENFVFTNDKKKSIILAFKEKQTS"
     gene            1658..1873
                     /locus_tag="NBI57_000203"
     CDS             1658..1873
                     /locus_tag="NBI57_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HCD1821191.1"
                     /translation="MNIQEATKLAMEKGISIRRENQDVYGILPTNLQRYQCLVVSRHY
                     KKKRQTAAGRWQPSADDLIADDWILDY"
     gene            complement(1862..2191)
                     /locus_tag="NBI57_000204"
     CDS             complement(1862..2191)
                     /locus_tag="NBI57_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001622080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1821192.1"
                     /translation="MTDEAKFVLLQLYSIYLGRIDEGMSKHSASYFGSDESSFNAFFL
                     GFNFEDYIDAVLELKHRDFVIASAEDGGFLEMALSREGIAYSESESKKDYKTLMGLIR
                     DLKKLII"
     gene            2242..2994
                     /locus_tag="NBI57_000205"
     CDS             2242..2994
                     /locus_tag="NBI57_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001148641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor KilAC domain-containing
                     protein"
                     /protein_id="HCD1821193.1"
                     /translation="MQALQTKSNIGEMFNIQEKENGEIAISGRELHQALEVKTPYKKW
                     FERMSDYGFEENIDYIVTDIFVHNPLGGRQNQTDHALTLDTAKEIAMIQRSEPGKRAR
                     QYFIQVEKAWNSPEMIMQRALKIANNTINQLETKIERDKPKIVFADAVATTKTSILVG
                     ELAKIIKQNGINIGQRRLFEWLRQNGFLIKRKGVDYNMPTQYSMERELFKIKETSITH
                     SDGHTSISKTPKVTGKGQQYFVNKFLGEKQTS"
     gene            3010..3207
                     /locus_tag="NBI57_000206"
     CDS             3010..3207
                     /locus_tag="NBI57_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1821194.1"
                     /translation="MQAQNKKVIYYYYDEECNRRPVNIQYNDGYDLMIDQRFIEMTLE
                     RHPHLKNNFYGLIDGKEFKLD"
     gene            complement(3194..3574)
                     /locus_tag="NBI57_000207"
     CDS             complement(3194..3574)
                     /locus_tag="NBI57_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000762519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2513 domain-containing protein"
                     /protein_id="HCD1821195.1"
                     /translation="MKLDHDCVRHLLLEIETNKKIGEPLTEYNFKDNVVFGKYDFETV
                     MYALLKLEEAKYVSVKFGWEDGHIYGYTINDITWSGHEFLDNIRDNHTWKEVKKVANK
                     TTSMSVTLLSKLAFNYLTQKFNLT"
     gene            3629..3952
                     /locus_tag="NBI57_000208"
     CDS             3629..3952
                     /locus_tag="NBI57_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001806402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF771 domain-containing protein"
                     /protein_id="HCD1821196.1"
                     /translation="MPHILNVTVPIPETHVLITKDEYEELIAYSLDPVWNMSDLKKKL
                     KIASDETIKDRLLFHPRLEKELRAQGIVHYPDENFNRWRFNARRMHKFVDEHFNEIYK
                     GGHNK"
     gene            3949..4110
                     /locus_tag="NBI57_000209"
     CDS             3949..4110
                     /locus_tag="NBI57_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1270 family protein"
                     /protein_id="HCD1821197.1"
                     /translation="MSKTYKSYLVAVLCFTVLAIVLMPFLYFTTAWSIAGFASIATFI
                     FYKEYFYEE"
     gene            4205..4507
                     /locus_tag="NBI57_000210"
     CDS             4205..4507
                     /locus_tag="NBI57_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2482 family protein"
                     /protein_id="HCD1821198.1"
                     /translation="MTKNYKDMTQEEIKDLLSEKTAELYELAKEIKGESKFDILLFSS
                     IGVIDGDYLAGSSSVIGHTFDLAYLLDSTKSYKDIVNVLQMCKSQKILGIDDDKED"
     gene            4512..4772
                     /locus_tag="NBI57_000211"
     CDS             4512..4772
                     /locus_tag="NBI57_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000291506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1108 family protein"
                     /protein_id="HCD1821199.1"
                     /translation="MYYKTGDVCRKIFNVDGFDFQLRVKKRAYSVEIVVLDHEGNSID
                     GLLVSDENDLYTALDILKQSIYEWIENNTDEQDRLINLVMKW"
     gene            4781..5044
                     /locus_tag="NBI57_000212"
     CDS             4781..5044
                     /locus_tag="NBI57_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001638663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1821200.1"
                     /translation="MRDTERNILNIFKTLFDEYTLSNQRALLEIERNHHGYLSINFLH
                     YHDSYKTNNKLVQIHEINPDSHERIKNLIIEVLRGHRKIKKGA"
     gene            5053..6996
                     /locus_tag="NBI57_000213"
     CDS             5053..6996
                     /locus_tag="NBI57_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCD1821201.1"
                     /translation="MKINKLTISNFAGIKEEKFNFDGKDAKIYGNNATGKTTTATALQ
                     WLLFDKGLDGSTKSFNPVPLNEKNEENYELIPTVFAEFEIDGKITTFKKESHPKYTIN
                     QKTNRKEYSRSRTKKQYINDESIKVKDYKARIDELIDEDVFKLITNPQAFNLLDWKKR
                     RSLLFEIAKPINDEDVIKTNDDFKELNNILGDHEIETKKKILTDKIKQINKDIKDIPI
                     RINQTQQNKQDVPEFDNDRHTIIKQEIEQLENERIDIQNGAEEINLRNQLADKQSELK
                     RIEANNSASNENKIHALTNELHVENGTVANLKTRLKQNKQQITHEENRRNQLLENHKG
                     LKSDLEKAKNQKFEYLDDNVCSCCGQQLPAEQVSEVREKALQKFNANKSKELETIQTS
                     INHIISEGKKIKPIIEKLEDDNNNLQIKINEAEERSARIQNKINKLKITHVDVTQTDE
                     YKAVMLEINEINQKRSNIRKTIQDKVSGIDDKISELTQEKSEIEVSISIEKSNKHLDD
                     VISELRNEEDRLLDEKEKYSHDLYILKEFTTTKVKMLTENINNEFDIAEFKLFNTLVN
                     GELEETCSTTVNGVEYDSGLNNASRINVGLDIINTLSKHFKVTAPIFIDNAESVTELI
                     KTESQQIQLIVNEQDKKLRMETI"
     gene            6998..7918
                     /locus_tag="NBI57_000214"
     CDS             6998..7918
                     /locus_tag="NBI57_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000180600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecT"
                     /protein_id="HCD1821202.1"
                     /translation="MTNELLLKNNKMGDNVLSRVKTLEAQGDLQFPANYSPENAMKSA
                     MLQLQELKGSKKDGYKPALEFATSTSIANALMDMVVQGLNPAKNQGYFIMYGDKVQFQ
                     RSYHGTMAVTKRVAGAEEINAEVIFEGDEVKYKTKNGKIVELEHTQSFGNRNTQNIIG
                     AYATVVFKDESRNYTEIMTFEEIEEAWKQSQMVYNGVFKEDGTHRRFPQEMAKKTVIN
                     RACKKILNSTDDASLLSNQIKESEQRQRKEVLDAEVEENANQEQLDFEPPVFEEAQYT
                     ELENEKPIDVSDFEEIKEPATEKESEEEPF"
     gene            7999..8616
                     /locus_tag="NBI57_000215"
     CDS             7999..8616
                     /locus_tag="NBI57_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001169067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HCD1821203.1"
                     /translation="MKFERVQKHFKYKTRHIAGCLITHEHGDHAKYTKQFVDNGVISY
                     MTAGTQRAMDFESHRLCTIKAKQELRIGTWSILPFDIEHDANEPVAFLLQSTLGYKVL
                     YVTDTKYLKYKFNGITHMMLEVNYIYEQMQENIKNGSVHSALANRIMESHFSLEHAIG
                     MLKANDLTRLEEIHLIHLSSQNSNAKYIKSEIQKVTGAPVYVGGL"
     gene            8617..9087
                     /gene="ssb"
                     /locus_tag="NBI57_000216"
     CDS             8617..9087
                     /gene="ssb"
                     /locus_tag="NBI57_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001837429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HCD1821204.1"
                     /translation="MLNRTILVGRLTRDPELRTTQSGVNVASFTLAVNRTFTNAQGER
                     EADFINIIVFKKQAENVNKYLSKGSLAGVDGRLQTRNYENKEGQRVYVTEVIADSIQF
                     LEPKNSNDTQQDLYQQQVQQTRGQSQYSNNKPVKDNPFANANGPIEIDDNDLPF"
     gene            9117..10010
                     /locus_tag="NBI57_000217"
     CDS             9117..10010
                     /locus_tag="NBI57_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain-containing protein"
                     /protein_id="HCD1821205.1"
                     /translation="MTGWISIDRSIQNHWLFKEKRTFSKFEAWIYLLMEANHSKAKVP
                     IGNQIVTVERGQRLTSILTLSDLFNWSRFKVKTFLDLLESDGMLEVKTTSKYTLITIV
                     NYDFYQSEQGRNQHQNDIKPTSKQHQSNINPTSKQHQTNTNNNDNKDNNEKNVNNEKK
                     KTTAFDFFQDNGFGFITSYNLDDLNYYLDSFENDSDEIVTASLKIAKDRNKVTWGYAK
                     SILNTWLNANLKSIEQVRAFEKQQLESKKQNYKPFVKQSKEKTPKWLTDSTRETKTPE
                     VDENLEKDREAFINRLNSKWE"
     gene            10017..10235
                     /locus_tag="NBI57_000218"
     CDS             10017..10235
                     /locus_tag="NBI57_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000338527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1821206.1"
                     /translation="MDAFDKYYLFDHDGNKMFSVTPHFKDGRHLVVGIKETKFNGRRW
                     YLDDYELNTLIDNEQMELGHQTSLFEYI"
     gene            10244..10648
                     /locus_tag="NBI57_000219"
     CDS             10244..10648
                     /locus_tag="NBI57_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000401967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HCD1821207.1"
                     /translation="MEIEIKFNEVFNAPMGSPRPRFRNTGRFVQTYMPTSYTKHKAYI
                     QGQMPKLNLERALKIELDFYFPLLKSWSKKKKSEMVGQYKVTKPDIDNLIKTVLDACN
                     GHVWKDDNQITEITSSKRYGIEPKIIIRIEEI"
     gene            10661..11032
                     /locus_tag="NBI57_000220"
     CDS             10661..11032
                     /locus_tag="NBI57_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001651750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1821208.1"
                     /translation="MARKARIVTINDKPYRFSKFEMELIESHGITAGMVSKRVKDGWE
                     LHEAMDAPEGTRLSEYREKKTIERLEQARLERKLERKRKREAELRRKKPHLFNVPQKH
                     SRDPYWFDNTYNQMFKKWSEA"
     gene            11032..11289
                     /locus_tag="NBI57_000221"
     CDS             11032..11289
                     /locus_tag="NBI57_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000111496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3310 domain-containing protein"
                     /protein_id="HCD1821209.1"
                     /translation="MSVISNRKVDMNEIQDNVKQPAHYTYGDIEIIDFIEQVTAQYPP
                     QLAFAIGNAIKYLSRAPLKNGHEDLAKAKFYVQRAFDLWEQ"
     gene            11286..11534
                     /locus_tag="NBI57_000222"
     CDS             11286..11534
                     /locus_tag="NBI57_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1821210.1"
                     /translation="MTMTYNARKEYLNQFFGSKRYLYQDNERVAHIHVVNGTYYFHGH
                     IVPGWQSVKKTFDTAEELEIYIKQHGLEYEEQKQLTLF"
     gene            11549..11794
                     /locus_tag="NBI57_000223"
     CDS             11549..11794
                     /locus_tag="NBI57_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1024 family protein"
                     /protein_id="HCD1821211.1"
                     /translation="MNNREQIEQSVISASAYNGNDTEGLLKEIEDVYKKARAFDEILD
                     GMTNAIQHSVKEGIELDEAVGIMAGQVIYKYEEEQGK"
     gene            11791..12327
                     /locus_tag="NBI57_000224"
     CDS             11791..12327
                     /locus_tag="NBI57_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000185675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTPase"
                     /protein_id="HCD1821212.1"
                     /translation="MTNTLTIDQLQELLQIQKKFDDRIPTRNLNDTVASMIIEFAEWV
                     NTLEFFKNWKKQPGKPLDTQLDEIADYLAFSLQLTLTIVDEEDLEETTEVMVDLIENE
                     VTLPKLHSVYFVHVMHTLTEQFVKGIDNSIVQVLIMPFLYANTYYTIDQLIDAYKKKM
                     KRNHERQDGTADAGKGYV"
     gene            12364..12609
                     /locus_tag="NBI57_000225"
     CDS             12364..12609
                     /locus_tag="NBI57_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001282077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1821213.1"
                     /translation="MSDMLEIFLIGFGVYLFYRIAIIFLKSKKTIHTNIYEMLMLATI
                     FMISTIAYKHQKTHILIAFLVMFFMSKLKQVQGSYEE"
     gene            12606..12812
                     /locus_tag="NBI57_000226"
     CDS             12606..12812
                     /locus_tag="NBI57_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1381 domain-containing protein"
                     /protein_id="HCD1821214.1"
                     /translation="MTQYLVTTFKDSTGQPHEHFTTARDNQTFTVVEAESKEEAERKY
                     EAQVKRGAVIKLGQLFENIRECGK"
     gene            12809..12958
                     /locus_tag="NBI57_000227"
     CDS             12809..12958
                     /locus_tag="NBI57_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCD1821215.1"
                     /translation="MIKQILRLLFLLAMYELGKYVTEQVYIMMTANDDVEAPSDYVFR
                     AEVSE"
     gene            12958..13158
                     /locus_tag="NBI57_000228"
     CDS             12958..13158
                     /locus_tag="NBI57_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001557462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1514 family protein"
                     /protein_id="HCD1821216.1"
                     /translation="MWITMTIVFAILLLVCISINSDHAREIQALRYMNDYLLDEVVKT
                     KGYNGLEEYRIELKRINNDIKK"
     gene            13186..13602
                     /locus_tag="NBI57_000229"
     CDS             13186..13602
                     /locus_tag="NBI57_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000590121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCD1821217.1"
                     /translation="MIKIEKHDIKKLEEYIQHIDNYRRELKMREYELLESHEPDNAGA
                     GKSNLPGNPIERCAIKKFSDNRYNTLRNIVNGVDRLIDESDEDTLELLRFRYWDCPIG
                     CYEWEDIAHYFGTSKTSILRRRNALIDKLAKYIGYV"
     gene            13834..14133
                     /locus_tag="NBI57_000230"
     CDS             13834..14133
                     /locus_tag="NBI57_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000988331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HCD1821218.1"
                     /translation="MMTKDERIRFYKSKEWQTTRKRVLERDNYECQQCKRDGKLTTYD
                     KSKHKSLDVDHILSLEHHPEFAHDLNNLETLCIKCHNKKEKRFIKKENKWKDEKW"
     gene            14263..14607
                     /locus_tag="NBI57_000231"
     CDS             14263..14607
                     /locus_tag="NBI57_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017804894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1821219.1"
                     /translation="MEIIVDENLVLKEKERLQVLYKDIPSNKLKVVDGLIIQAARLRV
                     MLDYMWEDIKEKGDYDLFTQSEKAPPYERERPVAKLFNARDAAYQKIIKQLSDLLPEE
                     KEDTETPSDDYL"
     gene            14604..16265
                     /locus_tag="NBI57_000232"
     CDS             14604..16265
                     /locus_tag="NBI57_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016187779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HCD1821220.1"
                     /translation="MISNKYVDEYINLWKQGKIILNKERIDLFNYLQKHIYSRDDVYF
                     DEQKIEDCIKFIEKWYFPTLPFQRFIIANIFLIDKNTDEAFFTEFAIFMGRGGGKNGL
                     ISAISDFLSTPLHGVKEYHISIVANSEDQAKTSFDEIRTVLMDNKRNKTGKTPKAPYE
                     VSKAKIINRATKSVIRYNTSNTKTKDGGREGCVIFDEIHYFFGPEMVNVKRGGLGKKK
                     NRRTFYISTDGFVREGYIDAMKHKIASVLSGKVKNSRLFAFYCKLDDPKEVDDRQTWE
                     KANPMLHKPLSEYAKTLLSTIEEEYNDLPFNRSNKPEFMTKRMNLPEVDLEKVIAPWK
                     EILATNREIPNLDNQMCIGGLDFANIRDFASVGLLFRKNDDYIWLGHSFVRQGFLDDV
                     KLEPPIKEWEKMGLLTIVDDDVIEIEYIVDWFLKAREKYGLEKVIADNYRTDIVRRAF
                     EDAGIKLEVLRNPKAIHGLLAPRIDTMFAKHNVIYGDNPLMRWFTNNVAVKVKPDGNK
                     EYIKKDENRRKTDGFMAFVHALYRADDIVDKDMSKALDALMSIDF"
     gene            16281..17468
                     /locus_tag="NBI57_000233"
     CDS             16281..17468
                     /locus_tag="NBI57_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000025279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HCD1821221.1"
                     /translation="MSILEKIFKTRKDITYMLDLDMIEDLSQQAYVKRLAIDSCIEFV
                     ARAVAQSHFKVLEGNRIQKNDVYYKLNIKPNTDLSSDSFWQQVIYKLIYDNEVLIVVS
                     DSKELLIADSFYREEYALYDDIFKDVTVKDYTYQRTFTMQEVIYLKYNNNKVTHFVES
                     LFEDYGKIFGRMIGAQLKNYQIRGILKSASSAYDEKNIEKLQAFTNKLFNTFNKNQLA
                     IAPLIEGFDYEELSNGGKNSNMPFSELSELMRDAIKNVALMIGIPPGLIYGETADLEK
                     NTLVFEKFCLTPLLKKIQNELNAKLITQSMYLKDTRIEIVGVNKKDPLQYAEAIDKLV
                     SSGSFTRNEVRIMLGEEPSDNPELDEYLITKNYEKANSGENDEKEKDENTLKGGDEDE
                     SGD"
     gene            17452..18189
                     /locus_tag="NBI57_000234"
     CDS             17452..18189
                     /locus_tag="NBI57_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001607394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease ClpP"
                     /protein_id="HCD1821222.1"
                     /translation="MKAEIKGVIVSNEDKWVYEMLGMDSTCPKDVLTQLEFSDEDVDI
                     IINSNGGNLVAGSEIYTHLRAHKGKVNVRITAIAASAASLIAMAGDHIEMSPVARMMI
                     HNPSSIAQGEAKDLNHAAETLEHVGQIMAEAYAVRAGKNKQELVEMMARETWLNADEA
                     IEQGFADSKMFENDNMQIVASNTQVLSKDVLNRVTALVSKTPEVNIDIDAIANKVIEK
                     INMKEKESEIDVADSKVSANGFSRFLF"
     gene            18213..19358
                     /locus_tag="NBI57_000235"
     CDS             18213..19358
                     /locus_tag="NBI57_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001573791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HCD1821223.1"
                     /translation="MTINLSETFANAKNEFINAVNNGEPQERQNELYGDMINQLFEET
                     KLQAKAEAERVSSLPKSAQSLSANQRSFFMDINKNVNYKEEKLLPEETIDRIFEDLTT
                     NHPLLADLGIKNAGLRLKFLKSETSGVAVWGKIYGEIKGQLDAAFSEETAIQNKLTAF
                     VVLPKDLNDFGPAWIERFVRVQIEEAFAVALETAFLKGTGKDQPIGLNRQVQKGVSVT
                     EGAYPEKEEQGTLTFANPRATVNELTQVFKYHSTNEKGKSVAVKGNVTMVVNPSDAFE
                     VQAQYTHLNANGVYVTALPFNLNVIESTVQEAGKVLTYVKGLYDGYLAGGINVQKFKE
                     TLALDDMDLYTAKQFAYGKAKDNKVAAVWKLDLKGHKPALEGTEETL"
     gene            19378..19638
                     /locus_tag="NBI57_000236"
     CDS             19378..19638
                     /locus_tag="NBI57_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000238242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1821224.1"
                     /translation="MVKFKVVRAFKDIEHNQHKYKVGELYPAEGYNNPRVELLTNQIK
                     NKYDKVYIVPLDKLTKQELLELCESLQKKRLVQWLKVKSSTY"
     gene            19651..19935
                     /locus_tag="NBI57_000237"
     CDS             19651..19935
                     /locus_tag="NBI57_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009560502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail adapter protein"
                     /protein_id="HCD1821225.1"
                     /translation="MTIDDLLVKFKSLEKIDHNSEDEYLKQLLKMSYERIKNQCGVFE
                     LENLIGQELILIRARYAYQDLLEHFNDNYRPEIIDFSLSLMEVSEDEESV"
     gene            19919..20281
                     /locus_tag="NBI57_000238"
     CDS             19919..20281
                     /locus_tag="NBI57_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000755150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail adaptor protein"
                     /protein_id="HCD1821226.1"
                     /translation="MKKVFKKPRITTKRLNTRVHFYKYTENNGPEAGEKEEKLLYSCW
                     ASIDGVWLRELEQAISNGTQNDIKLYIRDPQGDYLPSEEHYLEIESRYFKNRLNIKQV
                     SPDLDNKDFIMIRGGYSS"
     gene            20278..20682
                     /locus_tag="NBI57_000239"
     CDS             20278..20682
                     /locus_tag="NBI57_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000114224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HCD1821227.1"
                     /translation="MSVKVIGDKALERELEKRFGIKEMVKVQDKALIAGAKVIVEEVK
                     KQLKPSKDTGALINEVSFSKPEWINGKRTITVHWRGSKDRYKIVHLIEYGHVQKGTGK
                     FIKPKAMGGVNRAIRQGQNKYFETLKRELKKL"
     gene            20679..21086
                     /locus_tag="NBI57_000240"
     CDS             20679..21086
                     /locus_tag="NBI57_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1821228.1"
                     /translation="MIDILYKVHEVISQDRIIREHVNINNIKFNKYPNVKDTDVPFIV
                     IDDIDDPIPTTYTDGDECAYSYIVQIDVFVKYNDEYNARIIRNKISNRIQKLLWSELK
                     MGNVSNGKPEYIEEFKTYRSSRVYEGIFYKEEN"
     gene            21087..21731
                     /locus_tag="NBI57_000241"
     CDS             21087..21731
                     /locus_tag="NBI57_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000268736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCD1821229.1"
                     /translation="MAVKHASAPKAYINITGLGFAKLTKEGAELKYSDITKTRGLQKI
                     GVETGGELKTAYADGGPIESGNTDGEGKISLQMHAFPKEIRKIVFNEDYDEDGVYEEK
                     QGKQNNYVAVWFRQERRDGTFRTVLLPKVMFTNPKIDGETAEKDWDFSSEEVEGEALF
                     PLVDNKKSVRKYIFDSANMTNHDGDGEKGEEAFLKKILGEEYTGNVTEGNEETL"
     gene            21773..21997
                     /locus_tag="NBI57_000242"
     CDS             21773..21997
                     /locus_tag="NBI57_000242"
                     /inference="COORDINATES: protein motif:HMM:NF014427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HCD1821230.1"
                     /translation="MPDSIKTLKVGDTYDLNVVVEPSNQSKLLKYTTDQTNIVSINSD
                     GQVTAEAQGIATVKATVGNMSDTITINVEA"
     gene            22047..22397
                     /locus_tag="NBI57_000243"
     CDS             22047..22397
                     /locus_tag="NBI57_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1821231.1"
                     /translation="MAKLKRNIIQLVEDPKANEIKLQTYLTPHFISFEIVYEAMDLID
                     DIEDENSTMKPREIADRLMDMVVKIYDNQFTVKDLKERMHAPDGMNALREQVIFITQG
                     QQTEETRNFIQNMK"
     gene            22642..27171
                     /locus_tag="NBI57_000244"
     CDS             22642..27171
                     /locus_tag="NBI57_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001799555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HCD1821232.1"
                     /translation="MGERIKGLSIGLDLDAANLNRSFAEIKRNFKTLNSDLKLTGNNF
                     KYTEKSTHSYKQRIKELDGTITGYKKNVDDLAKQYGKVSQEQGENSAEAQKLRQEYNK
                     QANELNFLEKELEKTTTEFEEFKKAQVEAQRMAESGWGKTSKVFESMGPKLTKMGDGL
                     KSIGKGLMIGVTAPVLGIAAASGKAFAEVDKGLDTVTQATGATGGELKKLQNSFKDVY
                     GNFPEDAETVGGVLGEVNTRLGFTGKELESATESFLKFSHITGSDGVQAVQLITRAMG
                     DAGIEADEYQSVLDMVAKAAQASGISVDTLADSITKYGAPMRAMGFEMKESIALFSQW
                     EKSGVNTEIAFSGLKKAISNWGKAGKDPREEFKKTLAEIERTPDIASATSLAIEAFGA
                     KAGPDLADAIKGGRFSYQEFLKTIEDSQGTVNQTFKDSESGSERFKVAMNKLKLVGAD
                     VWASIESAFAPVMEELIKKLSVAVDWFSSLSDGSKRSIVIFGGIAAAIGPVVFGLGAF
                     ISTVGNAVTVLAPLLASIAKAGGLISFLSTKVPILGTVFTALTGPIGIVLGVLAGLAV
                     AFTIAYKKSETFRNFVNGAIESVKQTFSNFIQFIQPFIDSVKNIFKQAISAIVDFAKD
                     IWSQINGFFNENGISIVQALQNICNFIKAIFEFILNFVIKPIMFAIWQVMQFIWPAVK
                     ALIVSTWENIKGVIQGAINIILGIIKVFSSLFTGNWRGVWDGIVMILKGTVQLIWNLI
                     QLWFVGKILGVVRYFGGLLKGLITIIWVAIIGVFKKSLSAIWNATKSIFGFLFNSVKS
                     IFTNMKNWLSSTWNNIKSNTVGKAHSLFTGVRSKFTSLWNATKDIFIKLRNWMSNIWN
                     SIKDNTVGIAGRLWDRVRNIFGSMRDGLKSIIGKIKDHIGGMVDAVKRGLNKLIEGLN
                     WVGGKLGMDKIPKLHTGTEHTHTTTRLVKNGKIARDTFATVGDKGRGNGPNGFRNEMI
                     EFPNGKRVLTPNTDTTAYLPKGSKVYNGAQTYSMLNGTLPRFSIGTMWKDIKSGASSA
                     FNWTKDQIGKGTKWLGDKVGDVLDFIENPGKLLNYILEAFGIDFNSLTKGMGIAGDIT
                     KAAWSKIKKSATDWIKENLEAMGGGDLVGGILDPDKINYHYGRTAAYTAATGRPFHEG
                     VDFPFVYQEVRTPMGGRLTRMPFMSGGYGNYVKITSGVIDMLFAHLKNFSKSPPSGTM
                     VKPGDVVGLTGNTGFSTGPHLHFEMRRNGRHFDPEPYLRNAKKKGRLSIGGGGATSGS
                     GATYASRVIRQAQSILGGRYKGKWIHDQMMRVAKRESNYQSNAVNNWDINAQRGDPSR
                     GLFQIIGSTFRANAKRGYTNFNNPVHQGISAMQYIVRRYGWDGFKRAGDYAYATGGKV
                     FDGWYNLGEDGHPEWVIPTDPARRNDAMKMLHYAAAEVRGKNASKNKRPSQLSSVNGF
                     DDPSLLLKMIEQQQQQIALLLKIAQSNDVIADKDYQPIIDEYAFDKKVNASIEKRERQ
                     ESTKVKFRKGGIAIQ"
     gene            27168..28652
                     /locus_tag="NBI57_000245"
     CDS             27168..28652
                     /locus_tag="NBI57_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail family protein"
                     /protein_id="HCD1821233.1"
                     /translation="MIDTIKVNNKTIPWLYVERGFEIPSFNYVLKTENVDGRSGSIYK
                     GRRLESYSFDIPLVVRNDYLSHNGIKTHDDVLNELVKFFNYEEQVKLQFKSKDWYWNA
                     YFEGPIKLHKEFAIPVKFTIKVVLTDPYKYSVTGNKNTAISDQVSVVNSGTADTPLIV
                     EARAIKPSSYFMITKNDEDYFMVGDDEVTKEVKDYMPPVYHSEFRDFKGWTKMITEDI
                     PSNDLGGKVGGDFVISNLGEGYKATNFPDAKGWVGAGTKRGLPKAMTDFQITYKCIVE
                     QKGKGAGRTAQHIYDSDGKLLASIGYENKYHDRKIGHIVVTLYNQKGDPKKIYDYQNK
                     PIMYNLDRIVVYMRLRRVGNKFSIKTWKFDHIKDPDRRKPIDMDEKEWIDGGKFYQRP
                     ASIIAIYSAKYNGYKWMEMNGLGSFNTEILPKPKGARDVIIQKGDLVKIDMQAKSVVI
                     NEEPMLSEKSFGSNYFNVDSGYSELIIQPENVFDTTVKWQDRYL"
     gene            28668..32450
                     /locus_tag="NBI57_000246"
     CDS             28668..32450
                     /locus_tag="NBI57_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000582141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1821234.1"
                     /translation="MIHVLDFNDKIIDFLSTDDPSLVRAIHKRNVNDNSEMLELLISS
                     ERAEKFRERHRVIIRDSNKQWREFIINWVQDTMDGYTEIECIASYLADITTAKPYAPG
                     KFEKKTTSEALKDVLSDTGWEVSEQTEYDGLRTTSWTSYQTRYEVLKQLCTTYKMVLD
                     FYIELSSNTVKGRYVVLKKKNSLFKGKEIEYGKDLVGLTRKIDMSEIKTALIAVGPEN
                     DKGKRLELVVTDDEAQSQFNLPMRYIWGIYEPQSDDQNMNETRLSSLAKTELNKRKSA
                     VMSYEITSTDLEVTYPHEIISIGDTVRVKHRDFNPPLYVEAEVIAEEYNIISENSTYT
                     FGQPKEFKESELREEFNKRLNLIHQKLNDNISNINTIVKDVVDGELEYFERKIHKSDT
                     PPENPVNDMLWYDTSNPDVAVLRRYWNGRWIEATPNDVEKLGGITREKALFSELNNIF
                     INLSIQHASLLSEATELLNSEYLVDNDLKADLQASLDAVIDVYNQIKNNLESMTPETA
                     TIGRLVDTQALFLEYRKKLQDVYTDVEDVKIAISDRFKLLQSQYTDEKYKEALEIIAT
                     KFGLTVNEDLQLVGEPNVVKSAIEAARESTKEQLRDYVKTSDYKTDKDGIVERLDTAE
                     AERTTLKGEIKDKVTLNEYRNGLEEQKQYTDDQLSDLSNNPEIKASIEQANQEAQEAL
                     KSYIDAQDDLKEKESQAYADGKISEEEQRAIQDAQAKLEEAKQNAELKARNAEKKANA
                     YTDNKVKESTDAQRKTLTRYGSQIIQNGKEIKLRTTKEEFNASKRTLSRLLADITVNA
                     MKGIYLRYDENGAITSHTIDKDGVKISGDKVDITANREFNVVANNINNKVGKNDIVNS
                     LNLSNEGLDINVNRIGIKGGNANRYVQVQNDFIELGGIVQRTWKGKRSTDDIFTRLKD
                     GHLRFRNNTAGGSLYMSHFGISTYIDGEGEDGGSSGTIQWWDKTYSDSGMNGITINSY
                     GGVVALTSDYNRIIIDSYASANIESREAPIYLSPNTKNKPGLNRFAFTLSNADSAYET
                     DGYIMFGSDENYKYGAGLRFSKRSNKGLVQVVNGDYATGGDTTIESGMGKFNLVKRRD
                     GNSYVSIQSYDLLAVGSDNAGDRVASNSIYKRTYSAPANLHITSAGTIGRATSAKKYK
                     ISIENQYINEDDQFSHSKEILKLPIRTWFDKYESEIMAKELESGKKLSDDTFKLSRHT
                     GLIAEEVEELGFNEFVIYDDNGEIEGIAYDRLWIHLIPIIKNQQSKIEKLEELINE"
     gene            32443..32595
                     /locus_tag="NBI57_000247"
     CDS             32443..32595
                     /locus_tag="NBI57_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1821235.1"
                     /translation="MNDSNQGLQANPQYTIHYLSQEITRLTQENAMLKAYIQEQNEKS
                     KSAEEE"
     gene            32641..32928
                     /locus_tag="NBI57_000248"
     CDS             32641..32928
                     /locus_tag="NBI57_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1821236.1"
                     /translation="MANEIIKKTERFILVQIDKEGTERVLYQDFVGSFTTSDSASYAQ
                     DFKSEENAKKIAETLNLLYQLTGNQNGVKVVKEVVDRTDLSSDKSVDSETM"
     gene            32984..33358
                     /locus_tag="NBI57_000249"
     CDS             32984..33358
                     /locus_tag="NBI57_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000340977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1821237.1"
                     /translation="MDDIQKIKKELSELVERVDDVEILANETADHVLELREEHKQHHN
                     ELRESHKELKDKQDKVVDENLEQTKILNRIEERYQTQVDVAQKNEEKTLAQNKWLVGA
                     IWALVTIVMIAVITASITALLP"
     gene            33779..34552
                     /gene="sep"
                     /locus_tag="NBI57_000250"
     CDS             33779..34552
                     /gene="sep"
                     /locus_tag="NBI57_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000750405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphylococcal enterotoxin type P"
                     /protein_id="HCD1821238.1"
                     /translation="MKKTTFILLSFIALTLITSPFVNCSEKSEEINGKDLQKKSELQG
                     TALSNLRQTYYHNGSAIIENKESNDQFLKNTILFNDFFTGHQWYNDLLVDLGSKDTAN
                     IYKGKKVDLYGVYYGYQCTGGTPFKTACMYGGVTLHDNNQLEEEKKVPINLWIDGKQN
                     TVPLGTVKTNKKEVTVQELDLQSRHYLHETYNLYNTDAFNGKIQRGLIEFHPSSGDSV
                     GYDLFGAQGQYPDTQLRIYRDNKTIKSKNMHIDIYLYTT"
     gene            34755..34931
                     /locus_tag="NBI57_000251"
     CDS             34755..34931
                     /locus_tag="NBI57_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative holin-like toxin"
                     /protein_id="HCD1821239.1"
                     /translation="MDRWWLSEYKEVVPMVALLKSLERRRLMITISTMLQFGLFLIAL
                     IGLVIKLIELSNKK"
     gene            complement(34984..35091)
                     /locus_tag="NBI57_000252"
     CDS             complement(34984..35091)
                     /locus_tag="NBI57_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1821240.1"
                     /translation="MIFILWCVNLYIEKGQHAQTCYPSEPVKKTVAILD"
     gene            35143..35397
                     /locus_tag="NBI57_000253"
     CDS             35143..35397
                     /locus_tag="NBI57_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HCD1821241.1"
                     /translation="MINWKIRMKQKSFWVAILSAIFLFAQNIAKAIGYDIQVYTEQLT
                     DGLNAILGFLVLTGVIQDPTTKGIGDSHQALEYEEPRRKY"
     gene            35409..36164
                     /locus_tag="NBI57_000254"
     CDS             35409..36164
                     /locus_tag="NBI57_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HCD1821242.1"
                     /translation="MKTYSEARARLRWYQGRYIDFDGWYGYQCADLAVDYIYWLLEIR
                     MWGNAKDAINNDFKNMATVYENTPSFVPQIGDVAVFTKGIYKQYGHIGLVFNGGNTNQ
                     FLILEQNYDGNANTPAKLRWDNYYGCTHFIRPKYKSEGLMNKITNKINPPAQKAVGKS
                     ASKITVGSKAPYNLKWSKGAYFNAKIDGLGATSATRYGDNRTNYRFDVGQAVYAPGTL
                     IYVFEIIDGWCRIYWNNHNEWIWHERLIVKEAF"
     gene            36355..36846
                     /gene="sak"
                     /locus_tag="NBI57_000255"
     CDS             36355..36846
                     /gene="sak"
                     /locus_tag="NBI57_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphylokinase"
                     /protein_id="HCD1821243.1"
                     /translation="MLKRSLLFLTVLLLLFSFSSITNEVSASSSFDKGKYKKGDDASY
                     FEPTGPYLMVNVAGVDSKGNELLSPHYVEFPIKPGTTLTKEKIEYYVEWALDATAYKE
                     FRVVELDPSAKIEVTYYDKNKKKEETKSFPITEKGFVVPDLSEHIKNPGFNLITKVVI
                     EKK"
     gene            37493..37827
                     /locus_tag="NBI57_000256"
                     /pseudo
     CDS             37493..37827
                     /locus_tag="NBI57_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500032.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
     gene            complement(37922..>38108)
                     /locus_tag="NBI57_000257"
     CDS             complement(37922..>38108)
                     /locus_tag="NBI57_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500656.1"
                     /note="secreted protein that specifically inhibits the
                     activation of neutrophils and monocytes by binding to the
                     formylated peptide receptor and the C5a receptor; blocks
                     neutrophil migration towards the infection site and
                     hinders the establishment of the initial defense against
                     the infection; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="chemotaxis protein"
                     /protein_id="HCD1821244.1"
                     /translation="KMVILTENKGYYTVYLNTPLAEDRKNVELLGKMYKTYFFKKGES
                     KSSYVINGPGKTNEYAY"
BASE COUNT        14380 a         5383 c         7445 g        10900 t
ORIGIN      
        1 actataatgc tgattgtttt tagcgagtga tatcaacatc ttcgataaag ctagcacgtc
       61 aaatttagca gaatgtaatt tttctttatc gatatcatat aagctacaca aattttctaa
      121 tttaaaacta gagattgcgt ggaagcttct aaagatattt atactatcga catacatgaa
      181 gtttggaaca ggtaagtcat aataattatt agtatttttt aatactgaaa tatcaaaaag
      241 tgcattatga gcaataatta aatgtgattc ttttaaaaga tagagaattt cttggtaaat
      301 atctggatat ttaggtgctt ttaatatgac atcttcaggt attttatgta ttttagcgtt
      361 tttcaagtta tatctattat taggaggatt aatataagat gaataaactt ttactattga
      421 taaatcctta attaaagata cagcaacttc gcaagggctg ttcatatgtt cattcatagt
      481 ttcaaagtct aagactgcaa tatcatattt tttcatttgc aagtgctcct tttataaaat
      541 aacttttcca attaacctca cactttcatt tctataaaag tgtagatcgt cgtaatcttt
      601 atttagtgaa actagagtca atctatcatc ttcaacaaag actttcttaa catacgcttc
      661 ttcttcaatg atgaatatac caatttgtcc attctttata ttgtgagttt tctccacaaa
      721 tatgatttcg ccatctttaa acataggttc catagaatca ccatttactt ttaacgctaa
      781 atcgtgtgtg gggataggtc ctttaaccat ttcagtaaat agcgtttcat cgtgtaaacg
      841 ttctcctaca ccagcagaga cgcaaccatt gacgttaact ggagttttct cctgtttata
      901 tgaattaata tctacaacgt tatctccttt agaattctgt tcttccaatt gttcatttgc
      961 atagttaagt acgttttctt ggcggggagg tgtgagtttg ttgtatatgg aagtgatgtc
     1021 gttatcgtct ttgtatgtag tatctatgtc gcttttacca acctcgaaaa catcagctat
     1081 cctttgtata acgccgtgag aggggttgga acgtaaattt aaataatcgc ttaaagtaga
     1141 tggttttatg ttaatgagtt cagcaagttt cttttgagac atatttgaat cgttgagaaa
     1201 ttttctaatg tttttggcta taataatatt tctttctttg ttcatattac ttacctcctt
     1261 tttttcttat tatacgaaat tttcatatca tagtaaagtt ttttacgaaa aaaacgtatt
     1321 taatgttgac aatacgaaaa tttcgtatta tattaggttt acgaaaggcg gtgacaacat
     1381 gaaaacatta aaagagttga ggactgatta cggattgact caaaaagagt taggagattt
     1441 atttaaggtc tcatcacgta caattcaaaa tatggaaaaa gactctacaa acattaaaga
     1501 tagtttactt tctaagtata tgagtgcttt taatgttaaa tatgatgata tttttttagg
     1561 taatgaatac gaaaatttcg tatttacgaa cgataaaaag aaatcaatta ttttagcatt
     1621 taaagaaaaa caaacatctt aataggagga ataacaaatg aacattcaag aagcaacgaa
     1681 gctagcgatg gagaaaggaa taagtataag gagagagaat caagatgtgt atgggatatt
     1741 accaactaat ttgcagcgtt atcaatgcct agtcgtatct agacactata agaaaaaaag
     1801 acaaaccgcc gccggaaggt ggcagcctag cgcagacgat ttaatagcag atgattggat
     1861 tttagattat taattttttc aaatctctaa ttaaacccat aagtgttttg taatcttttt
     1921 tggattctga ttctgagtag gcgatacctt ctcgagaaag agccatctca agaaaaccgc
     1981 catcttcagc agaagcaatt acaaaatctc tatgctttaa ttcaagaact gcatcgatat
     2041 agtcttcaaa attaaaacct aaaaagaaag cgttaaatga ggattcatca ctaccgaaat
     2101 aagatgcaga atgtttagac ataccttcgt caattctacc aaggtaaatt gaataaagtt
     2161 gtaaaaggac aaatttagct tcatcagtca taagtcattc acctccttaa taggagtata
     2221 gcagaaagga gcacaaacaa tatgcaagca ttacaaacaa aatcgaacat aggcgaaatg
     2281 ttcaacatac aagaaaaaga aaatggagaa atcgcaatca gcggtcgaga acttcatcaa
     2341 gcattagagg ttaagactcc atacaaaaaa tggtttgaaa gaatgagtga ttacggattt
     2401 gaagaaaata tcgattatat agtcacggac atttttgtcc ataacccact aggaggtcgt
     2461 cagaatcaaa ctgaccacgc actcacacta gacactgcaa aagagattgc aatgattcaa
     2521 cgtagtgaac ctggcaaacg tgcaagacaa tacttcatcc aagttgaaaa agcatggaat
     2581 agcccagaaa tgattatgca acgtgcttta aaaattgcta acaacacaat caatcaatta
     2641 gaaacaaaga ttgaacgtga taaaccaaaa attgtatttg cagatgcagt agctactact
     2701 aagacatcaa ttttagttgg agagttagca aagatcatta aacaaaacgg tataaacatc
     2761 gggcaacgca gattgtttga gtggttacgt caaaacggat tccttattaa acgcaagggt
     2821 gtggattata acatgcctac acagtattca atggaacgtg agttattcaa aattaaagaa
     2881 acatcaatca cacattcgga cggtcacaca tcaattagta agacgccaaa agtaacaggc
     2941 aaaggacaac aatactttgt taataagttt ttaggagaaa aacaaacatc ttaataggag
     3001 gaacgaacaa tgcaagctca aaacaaaaaa gtcatctatt actactatga cgaagaatgt
     3061 aatagacgac ccgttaatat tcaatacaac gatggctacg acttaatgat agaccagcgt
     3121 tttattgaaa tgacgcttga aagacatccg catttaaaaa ataactttta tggattaata
     3181 gatggaaaag aatttaagtt agattaaatt tttgtgttag ataattaaaa gctaatttgc
     3241 ttagcaatgt tacggacata ctagtggttt tgtttgcgac ttttttaact tctttccaag
     3301 tgtgattgtc tcggatatta tctaaaaatt catgccctga ccaagttata tcgttaattg
     3361 tataaccata aatatgtcca tcttcccaac cgaatttaac actaacatac tttgcttctt
     3421 ccagttttaa taatgcatac attacagttt caaaatcata ttttccaaat acaacattat
     3481 ctttgaaatt gtattcggtg agcggttcac caatcttttt attagtttca atttctaaca
     3541 aaagatgtct aacacaatca tgatctaatt tcatacttat cactacctta ggttgataac
     3601 aacattatac acgaaaggaa agatagaaat gccacatatt ttaaacgtaa cagttccaat
     3661 acctgaaaca cacgtgctta tcacaaaaga tgaatatgaa gagttaatag cttactcatt
     3721 agaccctgta tggaacatga gcgacttaaa gaagaaatta aaaattgcat ctgatgaaac
     3781 aatcaaagac aggttattat ttcaccctag actcgaaaaa gagttaagag cacaaggtat
     3841 cgtacattat cctgatgaga attttaatcg ttggaggttt aacgcaagaa ggatgcataa
     3901 gtttgtagat gaacatttta atgagattta caaaggaggg cacaacaaat gagtaaaact
     3961 tataaaagct acctagtagc agtactatgc ttcacagtct tagcgattgt acttatgccg
     4021 tttctatact tcactacagc atggtcaatt gcaggattcg caagtatcgc aacattcata
     4081 ttctataagg aatactttta tgaagaataa aaaaactgct acttgcgcca acaagtaaca
     4141 gtaacaaaca tttaagaaat aaaattcaag ttaaatataa aacgaaaaac ggaggaagtc
     4201 aaccatgact aaaaattata aagacatgac gcaggaagaa ataaaagact tattatctga
     4261 aaaaacggca gaattgtatg aattagcgaa agaaattaag ggagaaagta aatttgatat
     4321 tttgcttttc tcatcaatag gagttatcga cggagattat ttagcaggtt caagttctgt
     4381 gattggtcat acttttgatc ttgcttactt attggatagc actaagagtt ataaagatat
     4441 tgtcaatgtt ctccaaatgt gtaaatcaca aaaaattctc ggtatagatg acgacaagga
     4501 ggactaaaac aatgtattac aaaacgggtg acgtatgtcg aaaaatattt aatgtagatg
     4561 gctttgattt tcaattaaga gttaagaagc gagcatatag tgtcgaaata gtcgttttag
     4621 atcatgaagg aaattcaatt gacgggctac tagtttctga cgagaacgat ctatacacag
     4681 ctttagatat tttgaaacaa agtatttatg aatggattga aaataacaca gatgaacagg
     4741 acagactaat taacttagtc atgaaatggt aggtataagc atgagagata cagaaagaaa
     4801 tatattgaat atttttaaga cgttattcga cgaatatact ttgtcaaacc aacgagcatt
     4861 attggaaatt gaacgtaatc atcacggata cttatcgatt aatttcttgc actatcacga
     4921 cagttacaaa acaaacaata agcttgtgca gatacatgaa atcaatccag acagccatga
     4981 acgaataaaa aatttaatta tcgaggtgct aagaggtcat cggaagatta aaaaaggagc
     5041 atgaggaaag atatgaaaat aaataagtta actatatcga actttgctgg aatcaaagaa
     5101 gaaaaattta actttgacgg taaagatgca aaaatatacg gcaataatgc gactggcaag
     5161 actacaacag caaccgcatt acaatggctg cttttcgata agggtttaga cggttcaacc
     5221 aaatcattta accctgtacc tttaaacgaa aaaaacgaag aaaattatga gttaattccg
     5281 actgttttcg cagaatttga aatcgacgga aaaattacga cttttaaaaa agagtcacat
     5341 cctaagtaca caataaatca aaaaacgaat cgcaaggaat actcacgaag tcgaacgaag
     5401 aaacaatata tcaatgatga atcaataaaa gtaaaggatt ataaagctcg tattgatgaa
     5461 ctgattgatg aagatgtatt caagttaatt acgaaccctc aagcatttaa cttactagat
     5521 tggaagaaac gaagaagttt gttgtttgaa atcgctaaac caatcaatga tgaggatgtc
     5581 attaaaacaa atgatgattt taaagaacta aataatattc ttggagatca cgaaattgaa
     5641 acaaagaaaa agattcttac agacaagata aaacagatta acaaagatat caaagatatt
     5701 ccgatacgta ttaaccaaac gcaacaaaat aagcaggatg taccggaatt cgataatgat
     5761 agacacacaa tcataaaaca agaaattgag caacttgaaa atgagcgtat agatattcaa
     5821 aacggtgcag aagaaattaa tttgcgtaac caattagctg ataaacaatc agaattgaag
     5881 cgcatagaag ctaataatag cgccagtaat gagaacaaaa tacatgcttt aacaaatgag
     5941 ctacacgttg aaaatggaac ggttgcgaat cttaaaacaa gattaaagca aaacaaacaa
     6001 caaattacac atgaagaaaa tcgacgtaat caattattag aaaatcataa aggattaaaa
     6061 agtgatttag aaaaagctaa aaatcaaaaa tttgaatatc ttgatgacaa tgtatgtagt
     6121 tgttgtggtc aacagttacc agctgaacaa gtgagtgagg taagagaaaa agcattgcag
     6181 aaattcaatg caaacaaatc gaaagaatta gaaacaatac aaacatctat caatcacatt
     6241 atttcagagg gcaagaaaat aaagccaatt atcgagaaat tagaggatga caacaataat
     6301 ttacaaatta aaatcaacga agcagaagag cgttcagcaa gaatacaaaa caaaattaat
     6361 aagttgaaaa taactcacgt tgacgttacg caaactgacg aatacaaagc agtaatgtta
     6421 gagataaatg agattaatca aaaacgctct aacatcagga aaactattca agataaagtt
     6481 tcaggaatag atgacaaaat aagcgaactt actcaagaaa aatcagaaat tgaagtgtca
     6541 atatcaatcg aaaaatcaaa taaacatcta gatgatgtta tttctgaatt aagaaatgaa
     6601 gaagacagat tattggatga aaaagaaaag tattcacatg acctttatat cttaaaagaa
     6661 tttacaacaa caaaagtcaa aatgcttact gaaaacatca ataacgaatt tgatattgct
     6721 gaatttaagc tattcaatac cttagttaac ggcgaattag aagaaacatg ttcaacaacg
     6781 gttaatggtg tcgagtatga cagcggttta aataacgcct caagaattaa tgttggctta
     6841 gatatcatca acacactatc aaaacatttt aaagttacag cgccaatatt tattgataat
     6901 gctgaatcag taacagagct tatcaaaaca gaatcacaac aaattcaatt gatagtaaat
     6961 gaacaagata aaaaattaag aatggagact atataaaatg acgaatgaat tactattaaa
     7021 aaacaataaa atgggcgaca acgttctatc tagagttaag acattagaag cacaaggaga
     7081 tttacagttt cctgcaaact attcgcctga gaatgcaatg aagtcagcaa tgttacaact
     7141 gcaagaatta aaaggatcta aaaaagatgg ttataaacca gcgctggaat ttgcaacttc
     7201 aaccagcata gcaaacgcct taatggacat ggttgtacaa ggtttaaatc ctgctaagaa
     7261 tcaaggctat ttcattatgt atggcgataa ggttcaattc caaagaagtt accacggaac
     7321 aatggcagta actaaacgtg tagcaggcgc agaagaaatt aatgcagaag tcatatttga
     7381 aggtgacgaa gttaagtata aaactaaaaa cggaaaaatt gttgaacttg aacatacaca
     7441 gtcttttggt aacagaaaca cacaaaacat tatcggtgca tatgcaacag ttgtatttaa
     7501 agatgaaagt agaaattaca ctgaaatcat gacatttgaa gagattgaag aagcgtggaa
     7561 gcaatcacaa atggtttata acggtgtatt taaagaagac ggtacacaca gaagattccc
     7621 tcaagaaatg gctaaaaaga ctgtaataaa ccgtgcatgt aaaaagattt taaacagcac
     7681 ggatgacgct agtcttttat caaatcaaat taaagaatct gaacaacgtc aacgcaaaga
     7741 agtattggat gcagaagttg aagaaaatgc aaatcaagaa caattggatt ttgaaccacc
     7801 agtttttgaa gaagcacaat acacagaatt agaaaatgaa aaacctattg atgtatctga
     7861 ctttgaagaa ataaaagaac ctgcaacaga aaaagaaagc gaagaagagc cattttaatt
     7921 gaaacaatag caactggttc aagtggtaac tgctacgtct taaatgatgg acgtactacg
     7981 ttactgcttg aggcaggaat aaaatttgaa cgtgttcaaa agcatttcaa atataaaaca
     8041 agacatatag cagggtgtct tatcacacac gaacatggtg atcatgcaaa gtacacaaag
     8101 cagtttgtcg acaatggtgt aatcagctat atgactgctg gaacacaacg agctatggat
     8161 tttgaaagtc atcgcttatg cacgattaag gcaaagcaag agctacgaat tggtacgtgg
     8221 tcaattttac catttgacat tgaacatgat gctaacgagc ctgtggcttt cttattacaa
     8281 agcacattag gttataaggt cctgtatgtt actgatacga agtatctgaa atacaaattt
     8341 aacggcatta cgcacatgat gttagaagtt aattatatct atgaacaaat gcaagaaaac
     8401 ataaaaaacg gcagtgtaca cagcgcatta gcaaacagaa ttatggagtc tcattttagc
     8461 ttagaacatg ctatcggaat gttgaaagca aatgatttaa ctagactcga agaaatacat
     8521 ttaattcatt taagtagtca aaattcaaat gcaaaataca ttaaaagtga aatacaaaaa
     8581 gtgacgggcg cgcccgttta tgttggaggt ttataaatgc taaacagaac aatattagtt
     8641 ggtcgtttaa ctagagaccc agaattaaga accactcaaa gtggtgtaaa tgtagcatca
     8701 ttcacattag cagttaaccg cacatttacg aatgcacaag gagagcgcga ggcagacttt
     8761 attaatatca tcgtatttaa aaaacaagca gagaacgtta ataaatacct atctaaagga
     8821 tcgttggcgg gcgtagatgg taggttacaa acgcggaact atgaaaataa ggaaggtcaa
     8881 cgtgtatacg ttacggaagt tattgctgat agtattcaat ttttagaacc gaaaaactca
     8941 aatgacactc aacaagattt atatcaacaa caagtacaac aaacacgtgg acaatcgcaa
     9001 tattcaaata acaaaccagt aaaagataat ccgtttgcga atgcaaatgg tccgattgaa
     9061 atagatgaca atgatttacc attctaattt aaccggtttg aaagtgaggt gtgtatatga
     9121 ctggttggat aagtattgat cgctcaattc aaaatcattg gctatttaaa gaaaagagaa
     9181 cattttcaaa gtttgaagca tggatatatt tactcatgga agcgaatcat tcaaaggcaa
     9241 aagtgcctat tggaaaccaa attgtaaccg tagaaagagg acaaagatta acatcgattt
     9301 tgaccttgtc tgaccttttt aactggtcac gatttaaagt gaaaaccttc cttgacttac
     9361 tcgagagtga tggaatgtta gaagtcaaaa caacatcaaa atataccctt ataaccattg
     9421 tcaattatga cttttatcaa agtgagcagg gcaggaacca acatcaaaac gacatcaaac
     9481 caacatcaaa acaacatcag tcaaacatca acccaacatc aaaacaacat caaaccaaca
     9541 caaacaataa tgataataaa gataataatg aaaagaatgt gaataatgag aagaagaaga
     9601 caaccgcctt cgacttcttc caagataacg gattcggttt cataacttct tacaatttag
     9661 acgatttaaa ttattatctt gattcatttg aaaatgattc agatgaaata gttaccgcat
     9721 cacttaaaat cgctaaagac agaaacaaag ttacttgggg atatgctaaa agcattttga
     9781 atacatggct taatgcaaac ttgaaatcta ttgaacaagt acgtgcattt gaaaagcaac
     9841 aacttgaaag caaaaaacaa aattataaac ctttcgttaa acaatcaaaa gaaaaaacac
     9901 ccaaatggct cacagacagc acgagagaaa cgaaaacgcc ggaagtagat gaaaaccttg
     9961 agaaagacag agaagctttt attaatcgtc taaatagcaa atgggagtga ttgaaaatgg
    10021 atgcatttga taaatactat ctatttgatc atgacggcaa caaaatgttt tcagttacac
    10081 cacattttaa agatggtcgg catttagttg ttggaataaa agaaacaaaa tttaatggtc
    10141 gtcgttggta tttagacgat tatgaattaa atacacttat tgataatgaa caaatggagt
    10201 taggacacca aacaagctta tttgaatata tatgagggat tacatggaga tagaaattaa
    10261 atttaatgaa gtgtttaatg cgccgatggg gtcgcctcgt ccacgctttc gtaatacagg
    10321 tagatttgtt caaacttaca tgccaacgtc ttacacaaag cataaagcgt atatacaagg
    10381 gcaaatgcct aagttaaatc tagagcgcgc actaaaaatc gaattagact tttactttcc
    10441 attgcttaaa tcatggtcga agaaaaagaa aagcgaaatg gttgggcagt ataaagtgac
    10501 taagccggat atcgacaact taattaaaac ggtattagat gcttgtaatg gccatgtatg
    10561 gaaagacgat aaccaaatta cagaaataac tagctcaaag cgttatggaa ttgagcccaa
    10621 aataatcata cgaatagaag aaatataaga ggtggataaa atggcgagaa aagcaaggat
    10681 tgtaacaata aatgataaac cttataggtt cagtaaattt gaaatggaat taatagaaag
    10741 tcacggtata accgctggaa tggtttctaa gagagtaaaa gacggttggg aactacatga
    10801 agcaatggac gcaccagaag gtacgcgttt aagcgagtac agagaaaaga aaacaataga
    10861 aagactggaa caagctagac tcgaacgcaa attggaaaga aagcgaaaga gagaggctga
    10921 gctaagaaga aagaagccac acttgtttaa tgtacctcag aaacattcac gtgatccgta
    10981 ctggtttgat aatacttata accaaatgtt caagaaatgg agtgaagcat aatgagtgta
    11041 atcagtaaca gaaaagtaga tatgaatgaa atacaagaca atgttaagca accagcgcac
    11101 tacacatacg gcgacattga aattatagat tttatcgaac aggttacggc gcagtatcca
    11161 ccacaattag catttgcaat aggtaatgca atcaaatatc tatctagagc accgttgaaa
    11221 aacggacacg aggatttagc aaaggcgaag ttttatgtcc aaagagcttt tgacttgtgg
    11281 gagcaatgac tatgacatat aacgcgcgca aagaatactt aaaccaattt ttcggatcta
    11341 agagatatct gtatcaggat aacgaacgag tggcacatat ccatgtagtg aatggcactt
    11401 attactttca cgggcatatc gtgccaggtt ggcaaagcgt taaaaagaca tttgatactg
    11461 ctgaagagct cgaaatatat ataaagcaac atggtttgga atacgaggaa cagaagcaac
    11521 taactttatt ttagaggaga tggaaatgat gaataatcgt gaacaaattg aacaatccgt
    11581 tataagtgct agtgcgtata acggcaatga cacagaggga ttgctaaaag agattgagga
    11641 cgtgtataag aaagcgcgag catttgatga aatacttgat ggaatgacaa atgctattca
    11701 acattcagtt aaagaaggta ttgaacttga tgaagcagta ggaattatgg caggtcaagt
    11761 tatctataaa tatgaggagg aacaaggaaa atgactaaca cattaacaat tgatcagtta
    11821 caagagttat tacaaataca aaagaagttc gacgatagaa taccgactag aaatttaaat
    11881 gacacagtag ctagtatgat tattgaattt gcggagtggg ttaacacact tgagtttttt
    11941 aaaaattgga agaaacaacc aggtaagcca ttagatacac aattagatga gattgctgat
    12001 tacttagctt tcagtttgca attaactttg actattgttg atgaagaaga tttggaagaa
    12061 actactgagg ttatggttga tttgattgaa aatgaagtta ctttacctaa actacattca
    12121 gtttattttg ttcatgtaat gcatacacta acagaacaat ttgtaaaagg tattgataat
    12181 agtattgtac aagttttaat aatgcctttt ttgtacgcca atacttacta tacaatcgac
    12241 caactcattg acgcatacaa aaagaaaatg aaaaggaacc acgaaagaca agatggaaca
    12301 gcagacgcag gaaaaggata cgtgtaaaga catcttggat cgagtcaagg aggttttggg
    12361 gaagtgagcg acatgttaga aatattttta atagggtttg gcgtttatct cttttatcgc
    12421 atagcaatta tttttcttaa gagtaaaaag actatacaca caaacatata tgaaatgtta
    12481 atgcttgcta ctatctttat gatatctaca attgcttata aacatcaaaa gacgcatatc
    12541 ttaatagcat ttttagtaat gttttttatg agtaagctca aacaagttca agggagctat
    12601 gaggaatgac acaatactta gtcacaacat tcaaagattc aacaggacaa ccacatgaac
    12661 attttactac tgctagagat aatcagacgt ttacagttgt tgaggcagag agtaaagaag
    12721 aagctgagcg caaatacgag gcacaagtta aaagaggtgc agttattaaa ttagggcagt
    12781 tgtttgaaaa tataagggag tgtgggaaat gattaagcaa atactaagat tattattctt
    12841 actagcaatg tacgagttag gtaagtatgt aactgagcaa gtatatatta tgatgacagc
    12901 taatgatgat gtagaggcgc cgagtgatta cgtctttcga gcggaggtaa gtgagtgatg
    12961 tggattacta tgactattgt atttgctata ttgctattag tttgtatcag tattaatagt
    13021 gatcatgcaa gagagataca agcactcaga tatatgaatg attatctact tgatgaagta
    13081 gttaaaacta aaggatacaa cgggttagaa gaatacagga ttgaattgaa gcgaataaat
    13141 aacgatatta aaaagtaatt tatattatcg gaggtattgc attgaatgat aaagattgag
    13201 aaacacgata tcaaaaagct tgaagaatac attcagcaca tcgataacta tcgaagagag
    13261 ttgaagatgc gagaatatga attacttgaa agtcatgaac cagataatgc gggagctggc
    13321 aaaagtaatt tgccgggtaa tccgattgaa cgatgtgcaa taaagaagtt tagtgataac
    13381 aggtacaata cattaagaaa tatagttaac ggtgtagata gattgataga tgaaagtgat
    13441 gaggatacgc ttgagttatt aaggtttaga tattgggatt gtcctattgg ttgttatgaa
    13501 tgggaagata tagcacatta ctttggtaca agtaagacaa gtatattacg tagaaggaat
    13561 gcactgatcg ataagttagc aaagtatatt ggttatgtgt agcggacttt taccctatgt
    13621 aagtccgcat taaaacagtt tattatgtta gtatcagatt aatatttaag gttattaaat
    13681 gctaatacga cgcatgaaca agaggcgcat tactatgtga tgtgtctttt tatttatgag
    13741 gtatgaacat gttcaaacta attgtaaata cattactaca catcaagtat agatgcgtct
    13801 tgatactact taagttatat aaggtgaaac attatgatga ctaaagacga acgtatacga
    13861 ttctataagt ctaaagaatg gcaaacaaca agaaaaagag tactagaaag agataattat
    13921 gaatgtcaac aatgtaagag agacggcaag ttaacgacat atgacaaaag caaacataag
    13981 tcgttggatg tagatcatat attatcgcta gaacatcatc cggagtttgc tcatgactta
    14041 aacaatttag aaacactgtg tattaaatgt cacaacaaaa aagaaaagag atttataaaa
    14101 aaagaaaata aatggaaaga cgaaaaatgg taaatacccc cgggtcaaaa aaatcaaaag
    14161 cgatcaaaat acttggggaa cggggagggg ctcgacttcg cgataatttt aaaaatccat
    14221 gtataacccc cctcttataa ccattttaag gcaggtgatg aaatggagat tatagttgat
    14281 gaaaacttag tgcttaaaga aaaagaaagg ctgcaagtat tatataaaga catacctagc
    14341 aataaattaa aagtagttga tggtttaatt attcaagcag caaggctacg tgtaatgctt
    14401 gattacatgt gggaagacat aaaagaaaaa ggtgactatg atttatttac tcaatctgaa
    14461 aaggcgccac catatgaaag ggaaagacca gtagccaaac tatttaatgc tagagatgct
    14521 gcatatcaaa aaataatcaa acaattatcg gatttattgc ccgaagagaa agaagacaca
    14581 gaaacgccat ctgatgatta cctatgatta gtaataaata cgttgatgaa tatataaatt
    14641 tgtggaaaca aggaaagata attttaaata aagaaagaat tgatctcttt aattatctac
    14701 aaaaacatat atattcacga gatgatgtat attttgatga acagaaaatc gaggattgta
    14761 tcaaatttat tgaaaaatgg tattttccaa cattaccatt tcaaaggttt atcatagcta
    14821 atatatttct tatagataaa aatacagatg aagctttctt tacagaattt gctattttca
    14881 tgggacgtgg aggcgggaaa aacggtctaa taagtgctat tagtgatttt ctttctacgc
    14941 ccttacacgg agttaaagaa tatcacatct ccattgttgc taatagtgaa gatcaagcaa
    15001 aaacatcgtt tgatgaaatc agaaccgttt taatggataa caaacgaaat aagacgggta
    15061 aaacgccaaa agctccttat gaagttagta aagcaaaaat aataaaccgt gcaactaaat
    15121 cggttattcg atataacaca tcaaacacaa aaaccaaaga cggtggacgt gaggggtgtg
    15181 ttatttttga tgaaattcat tatttctttg gtcctgaaat ggtaaacgtc aaacgtggtg
    15241 gattaggtaa aaagaaaaat agaagaacgt tttatataag tactgatggt tttgttagag
    15301 agggttatat cgatgcaatg aagcacaaaa ttgcaagtgt attaagtggc aaggttaaaa
    15361 atagtagatt gtttgctttt tattgtaagt tagacgatcc aaaagaagtt gatgacagac
    15421 agacgtggga aaaggcgaac ccaatgttac ataaaccgtt atcagaatac gctaaaacac
    15481 tgctaagcac gattgaagaa gaatataacg atttaccatt caaccgttca aataagcccg
    15541 aattcatgac taagcgaatg aatttgcctg aagttgacct tgaaaaagta atagcaccat
    15601 ggaaagaaat actagcgact aatagagaga taccaaattt agataatcaa atgtgtattg
    15661 gtggtttaga ctttgcaaac attcgagatt ttgcaagtgt agggctatta ttccgaaaaa
    15721 acgatgatta catttggtta ggacattcgt ttgtaagaca agggtttttg gatgatgtca
    15781 aattagaacc tcctattaaa gaatgggaaa aaatgggatt attgaccatt gtcgatgatg
    15841 atgtcattga aattgaatat atagttgatt ggtttttaaa ggctagagaa aaatatgggc
    15901 ttgaaaaagt catagctgat aattatagaa ctgatattgt aagacgtgcg tttgaggatg
    15961 ctggcataaa acttgaagta cttagaaatc caaaagcaat acatggatta cttgcaccac
    16021 gtatcgatac aatgtttgcg aaacataacg taatatatgg agacaatcct ttgatgcgtt
    16081 ggtttactaa taatgttgca gtaaaggtta aacccgatgg taataaagaa tatattaaaa
    16141 aagatgaaaa tagaagaaaa accgatgggt tcatggcttt tgttcacgca ttatatagag
    16201 cagacgatat agtagacaaa gacatgtcta aagcgcttga tgcattaatg agtatagatt
    16261 tctaatagag gaggtgagac atgagtattc tagaaaagat atttaaaact aggaaagata
    16321 taacatatat gcttgattta gatatgatag aagatctatc acaacaagcg tatgtgaaac
    16381 gtttagcgat tgatagttgt attgaatttg ttgcgcgagc tgtcgctcaa agtcatttta
    16441 aagtattgga aggtaataga attcaaaaga atgatgttta ctacaagtta aatataaaac
    16501 caaatactga cttatcaagc gatagttttt ggcaacaagt tatatataaa ctaatttatg
    16561 ataacgaggt tttaatcgta gtaagtgaca gcaaagaatt acttatcgca gatagctttt
    16621 acagagaaga gtacgctttg tatgatgata tattcaaaga tgtaacggtt aaagattata
    16681 cttatcaacg tactttcaca atgcaagagg tcatatattt aaagtacaac aacaataaag
    16741 tgacacactt tgtagaaagt ctattcgaag attacgggaa aatattcgga agaatgatag
    16801 gtgcacaatt aaaaaactat caaataagag ggattttgaa atctgcctct agcgcatatg
    16861 acgaaaagaa tatagaaaaa ttacaagcgt tcacaaataa attattcaat acttttaata
    16921 aaaatcaact agcaatcgcg cctttgatag aaggttttga ttatgaggaa ttatctaatg
    16981 gtggtaagaa tagtaacatg cctttttctg aattgagtga gctaatgaga gatgcaataa
    17041 aaaatgttgc gttgatgatt ggtatacctc caggtttgat ttacggagaa acagctgatt
    17101 tggaaaaaaa cacgcttgta tttgagaagt tctgtttaac acctttatta aaaaagattc
    17161 agaacgaatt aaacgcgaaa ctcataacac aaagcatgta tttgaaagat acaagaatag
    17221 aaattgtcgg tgtgaataaa aaagacccac ttcaatatgc tgaagcaatt gacaaacttg
    17281 taagttctgg ttcatttaca aggaatgagg tgcggattat gttaggtgaa gaaccatcag
    17341 acaatcctga attagacgaa tacctgatta ctaaaaacta cgaaaaagct aacagtggtg
    17401 aaaatgatga aaaagaaaaa gatgaaaaca ctttgaaagg tggtgatgaa gatgaaagcg
    17461 gagattaaag gcgtcatcgt ttccaacgaa gataaatggg tttacgaaat gcttggtatg
    17521 gattcgactt gtcctaaaga tgttttaaca caactagaat ttagtgatga agatgttgat
    17581 attataatta actcaaatgg tggtaaccta gtagctggta gtgaaatata tacacattta
    17641 agagctcata aaggcaaagt gaatgttcgt atcacagcaa tagcagcaag tgcggcatcg
    17701 cttatcgcaa tggctggtga ccacatcgaa atgagtccgg ttgctagaat gatgattcac
    17761 aatccttcaa gtattgcgca aggagaagcg aaagatctaa atcatgctgc agaaacatta
    17821 gaacatgttg gtcaaataat ggctgaggca tatgcggtta gagctggtaa aaacaaacaa
    17881 gaacttgtag aaatgatggc tagggaaacg tggctaaatg ctgatgaagc cattgaacaa
    17941 ggttttgcgg atagtaaaat gtttgaaaac gacaatatgc aaattgtagc aagcaataca
    18001 caagtgttat cgaaagatgt attaaatcgt gtaacagctt tggtaagtaa aacgccagag
    18061 gttaacattg atattgacgc aatagcaaat aaagtaattg aaaaaataaa tatgaaagaa
    18121 aaggaatcag aaatcgatgt tgcagatagt aaagtatcag caaatggatt ttcaagattc
    18181 cttttttaat acaaaaaata ggaggtcata aaatgactat aaatttatcg gaaacattcg
    18241 caaatgcgaa aaacgaattt attaatgcag taaacaacgg tgaaccgcaa gaaagacaaa
    18301 atgaattgta cggtgacatg attaaccaac tatttgaaga aactaaatta caagcaaaag
    18361 cagaagctga aagagtttct agtttaccta aatcagcaca atctttgagt gcaaaccaaa
    18421 gaagtttctt catggatatc aataaaaacg ttaactataa agaagaaaaa cttttgccag
    18481 aagaaacaat tgatagaatt tttgaagatt tgacgacgaa tcatccgtta ttagctgatt
    18541 taggtattaa aaacgctggt ttgcgtttga agttcttaaa atctgaaact tctggcgtag
    18601 ccgtttgggg taaaatctat ggtgaaatta aaggtcaatt agatgctgcg ttcagtgaag
    18661 aaacagcaat tcaaaataaa ttgacagcgt ttgttgtttt accaaaagat ttaaatgatt
    18721 ttggtcctgc gtggattgaa agatttgttc gtgttcaaat cgaagaagca tttgcagtgg
    18781 cgcttgaaac tgcgttctta aaaggtactg gtaaagacca accaatcggc ttaaaccgtc
    18841 aagtacaaaa aggtgtatcg gtaactgagg gtgcttatcc agagaaagaa gaacaaggta
    18901 cgcttacatt tgctaatccg cgcgctacgg ttaatgaatt gacgcaagtg tttaaatacc
    18961 actcaactaa cgagaaaggt aaatcagtag cggttaaagg taatgtaaca atggttgtta
    19021 atccgtccga tgcttttgag gttcaagcac agtatacaca tttaaatgca aatggcgtat
    19081 atgttactgc tttaccattt aatttgaatg ttatcgagtc tacagtccaa gaagcaggta
    19141 aggttttaac gtacgttaaa ggtttatatg atggttattt agctggtggt attaatgttc
    19201 agaaatttaa agaaacactt gcgttagatg atatggattt atacactgca aaacaatttg
    19261 cttacggcaa agcgaaagat aataaagttg ctgctgtttg gaaattagat ttaaaaggac
    19321 ataagccagc tttagaaggt accgaagaaa cactataaaa ttttatgagg tgataaaatg
    19381 gtgaaattta aagttgttag agcttttaaa gacatagagc acaatcaaca caagtacaaa
    19441 gtaggggagt tgtatccagc tgaagggtat aacaatcctc gtgttgaatt gttgacaaat
    19501 caaatcaaaa ataagtacga caaagtttat atcgtacctt tagataagct gacaaaacaa
    19561 gaattattag aactatgcga atcattacaa aaaaagcgtc tagttcaatg gttaaaagtg
    19621 aaatcgtcga cttattgaat ggtgaagaca atgacgattg atgatttgct tgtcaaattt
    19681 aaatcacttg aaaagattga ccataattca gaggatgagt acttaaagca gttgttaaaa
    19741 atgtcgtacg agcgtataaa aaatcagtgc ggagtttttg aattagagaa tttaataggt
    19801 caagaattga tacttatacg cgctagatat gcttatcaag atttattaga acacttcaac
    19861 gataattaca gacctgaaat aatagatttt tcgttatctc taatggaggt atcagaagat
    19921 gaagaaagtg tttaaaaaac ctagaattac aactaaacgt ttaaatactc gtgttcattt
    19981 ttataagtat actgaaaata atggtccaga agctggagaa aaagaagaaa aattattata
    20041 tagctgttgg gcgagtattg atggtgtctg gttacgtgaa ttagaacaag ctatctcaaa
    20101 cggaacccaa aatgacatta aattgtatat tcgtgatccg caaggtgatt atttacccag
    20161 tgaagaacat tatcttgaaa ttgaatcaag atatttcaaa aatcgtttga atataaagca
    20221 agtatcacca gatttggata ataaagactt tattatgatt cgtggaggat atagttcatg
    20281 agtgtgaaag tgataggtga taaagcatta gaaagagaat tagaaaaacg ttttggcata
    20341 aaagagatgg taaaagttca agataaggcg ttaatagctg gtgctaaggt aattgttgaa
    20401 gaagtaaaaa aacaactaaa gccctcaaaa gatacgggag cattaattaa tgaggtaagt
    20461 tttagtaaac ctgaatggat aaacggaaaa cgtacaatta ctgttcattg gcgaggttct
    20521 aaagaccgtt ataaaatcgt acatttaatt gaatatggac acgttcaaaa aggaacaggt
    20581 aaatttatca aacctaaagc tatgggcggt gttaatagag caataagaca agggcaaaat
    20641 aagtattttg agacgctaaa aagggagttg aaaaaattgt gattgatatt ttgtacaaag
    20701 ttcatgaagt gattagtcaa gacagaatta ttagagagca cgtaaatatc aataatatta
    20761 agttcaataa ataccctaat gtaaaagata ctgatgtacc ttttattgtt attgacgata
    20821 tcgacgaccc aatacctaca acttatactg acggagatga gtgtgcatat agttatattg
    20881 tccaaataga tgtttttgtt aagtacaatg atgaatataa tgcgagaatc ataagaaata
    20941 agatatctaa tcgcattcaa aagttattat ggtctgaact aaaaatggga aatgtttcaa
    21001 atggaaaacc ggaatatata gaagaattta aaacatatag aagctctcgc gtttacgagg
    21061 gcatttttta taaggaggaa aattaaatgg cagtaaaaca tgcaagtgcg ccaaaggcgt
    21121 atattaacat tactggttta ggtttcgcta aattaacgaa agaaggcgcg gaattaaaat
    21181 atagtgatat tacaaaaaca agaggattac aaaaaattgg tgttgaaact ggtggagaac
    21241 taaaaacagc ttatgctgat ggcggtccaa ttgaatcagg gaatacagac ggagaaggta
    21301 aaatctcatt acaaatgcat gcgttcccta aagagattcg caaaattgtt tttaatgaag
    21361 attatgatga agatggcgtt tacgaagaga aacaaggtaa acaaaacaat tacgtagctg
    21421 tatggttcag acaagagcgt agagacggta catttagaac agttttatta cctaaagtta
    21481 tgtttacaaa tcctaaaatc gatggagaaa cggctgagaa agattgggat ttctcaagtg
    21541 aagaggttga aggtgaggca cttttccctt tagttgataa taaaaagtca gtacgtaagt
    21601 atatctttga ttcagctaac atgacaaatc atgatggaga cggtgaaaaa ggcgaagagg
    21661 ctttcttaaa gaaaatttta ggcgaagaat atactggaaa cgtgacagag ggtaacgaag
    21721 aaactttgta acaaaaccgg cttcatcgga aactgcggta aagtcggtta atataccaga
    21781 tagcattaaa acacttaaag ttggcgacac atacgattta aatgttgtag tagagccatc
    21841 taatcaaagt aagttattga aatacacaac agatcaaacg aatattgtat caatcaatag
    21901 tgatggtcaa gttactgcgg aagcacaagg cattgctacg gttaaagcaa cagttggtaa
    21961 tatgagtgac actataacaa taaatgtaga agcataagag ggggcaaccc ctctatttta
    22021 tttgaaaata aggagagtat tataaaatgg caaaattaaa acgtaacatt attcaattag
    22081 tagaagaccc gaaagcaaat gaaattaaat tacaaacgta cttaacacca cacttcattt
    22141 catttgaaat tgtatacgaa gcaatggatt taatcgatga tattgaggac gaaaatagca
    22201 cgatgaaacc aagagaaatc gctgacagat tgatggatat ggttgtaaaa atttacgata
    22261 accaattcac agttaaagac ctaaaagaac gtatgcatgc acctgatgga atgaatgcac
    22321 ttcgtgaaca agtgattttc attactcaag gtcagcaaac tgaggaaact agaaatttta
    22381 tccagaacat gaaataaagc ctgaagattt aacatataaa gcaatgttga aaaatatgga
    22441 tactctcatg atggacttaa ttgaaaatgg taaagacgct aacgaagttt taaaaatgcc
    22501 atttcattat gtactttcca tatatcaaaa taaaaacaat gacatttctg aagaaaaagc
    22561 agaggcttta attgatgcgt tttaacctta accgtttggt tagggttatt tttttgaact
    22621 tttttagaaa ggaggtaaaa aatgggagaa agaataaaag gtttatctat aggtttggat
    22681 ttagatgcag caaatttaaa tagatcattt gcagaaatca aacgaaactt taaaacttta
    22741 aattctgact taaagttaac cggtaacaac ttcaaatata ccgaaaaatc aactcatagt
    22801 tacaaacaaa ggattaaaga acttgatgga actatcacag gttataagaa aaacgttgat
    22861 gatttagcca agcaatatgg caaggtatct caagaacagg gcgaaaacag cgcggaagct
    22921 caaaaattac gacaagaata taacaaacaa gcaaatgagc tgaatttttt agaaaaagaa
    22981 ctagaaaaaa caacaactga gtttgaagag ttcaaaaaag ctcaagttga agctcaaaga
    23041 atggcagaaa gtggctgggg aaaaaccagt aaagtttttg aaagtatggg acctaaatta
    23101 acaaaaatgg gtgatggttt aaaatccatt ggtaaaggtt tgatgattgg tgtaactgca
    23161 cctgttttag gtattgcagc agcatcagga aaagcttttg cagaagttga taaaggttta
    23221 gatacagtta cccaagcaac aggagcaacc ggcggagagc ttaagaagtt gcagaattca
    23281 tttaaagatg tttatggcaa ctttccagaa gacgctgaga ctgtaggcgg tgttttaggg
    23341 gaagttaaca caaggttagg tttcactggc aaagaacttg agagtgccac agagtcattc
    23401 ttgaaattta gtcacataac aggttctgac ggcgtacaag ccgttcaatt aattacgcgt
    23461 gcaatgggtg atgcaggtat tgaagctgat gagtatcaaa gtgtacttga tatggtagcg
    23521 aaagcagcac aggctagcgg tataagtgtt gatacattag ctgatagcat tactaaatac
    23581 ggtgctccaa tgagggctat gggctttgag atgaaagaat caatcgcttt attctctcaa
    23641 tgggagaaat caggtgttaa tactgaaata gccttcagtg gtttgaaaaa agctatatcc
    23701 aattggggta aagcgggtaa agacccaaga gaagaattta agaagacatt agcagaaatt
    23761 gaaaggacac cggatatagc tagcgcaaca agtttagcga ttgaagcatt tggtgcaaaa
    23821 gcaggtcctg atttagcaga tgctattaaa ggcggtcgct ttagttacca agagttctta
    23881 aaaactatcg aagattcgca aggaacggtc aatcagacat ttaaagattc tgaaagtggc
    23941 tccgaaagat ttaaagtagc aatgaataaa cttaaattag taggtgctga tgtatgggct
    24001 tctattgaaa gtgcgtttgc tccagtcatg gaagaattaa tcaaaaagct atctgtagca
    24061 gttgattggt tttcaagttt aagtgatgga tctaaaaggt cgattgttat attcggtggt
    24121 attgctgctg caattggtcc tgtagttttt ggattaggtg cattcataag cacagttggc
    24181 aacgcagtaa ctgtattagc tccattatta gctagtattg caaaggctgg tggattgatt
    24241 agttttttat cgactaaagt acctatatta ggaactgtct tcacagcttt aactggtcca
    24301 attggcattg tattaggtgt attggctggt ttagcagtcg catttacaat tgcttataag
    24361 aaatctgaaa catttagaaa ttttgttaat ggtgcaattg aaagtgttaa acaaacattt
    24421 agtaatttta ttcaatttat tcaacctttc attgattctg ttaaaaacat ctttaaacaa
    24481 gcgatatcag caatagttga ttttgctaaa gatatttgga gtcaaatcaa tggattcttt
    24541 aatgaaaatg gaatttccat tgttcaagca cttcaaaata tatgcaactt tattaaagcg
    24601 atatttgaat ttattttaaa ttttgtaatt aaaccaatta tgttcgcaat ctggcaagtg
    24661 atgcaattta tttggccggc ggttaaagcc ttgattgtca gcacttggga aaatatcaaa
    24721 ggtgtaatac aaggggctat taatattatt ttgggtatta tcaaagtgtt ctctagtctt
    24781 ttcacaggaa actggcgagg cgtttgggac ggcattgtaa tgatactgaa aggtactgtg
    24841 cagttaattt ggaatttaat tcaattatgg tttgtaggca aaatacttgg cgttgttagg
    24901 tactttggcg gattgctaaa aggtttaata actattatat gggttgctat aataggcgtt
    24961 ttcaagaaat cattatcggc aatttggaat gcaacaaaaa gtatttttgg tttcttattc
    25021 aatagtgtta aatctatttt cactaatatg aaaaactggt tatctagtac gtggaataat
    25081 atcaaaagca ataccgtcgg caaggctcat tcgttattta cgggtgtaag gtctaaattc
    25141 acaagtttat ggaatgcgac gaaagatata tttattaaat taagaaattg gatgtcaaac
    25201 atctggaact ctattaaaga taacactgta ggtatagctg gtcgcttatg ggatagagtg
    25261 cgtaacatct ttggaagcat gcgtgacggt ttaaaatcta tcattggtaa aattaaagat
    25321 catatcggtg gtatggtaga cgctgttaaa agaggtctta ataaattaat tgaaggttta
    25381 aactgggtcg gtggtaagtt gggtatggac aaaataccga agttacacac tggtactgaa
    25441 catacgcata ctactacaag attagttaag aacggtaaga ttgcgcggga tacgttcgct
    25501 acggttgggg ataaaggacg tggaaatggt ccgaatggtt tcagaaatga aatgattgaa
    25561 ttccctaatg gcaaacgggt acttacgcct aatacagata cgacagcgta cttacctaaa
    25621 ggttcaaaag tatataacgg cgcacaaact tattcaatgt taaatggaac gcttccaaga
    25681 tttagcatag gtactatgtg gaaagatatt aaatccggtg catcatcggc atttaactgg
    25741 acaaaagatc aaataggtaa aggtaccaaa tggcttggcg ataaagttgg cgatgtttta
    25801 gattttattg aaaatccagg caaactttta aattatatac ttgaagcttt tggaattgat
    25861 ttcaattctt taactaaagg tatgggaatt gcaggcgaca taacaaaagc tgcatggtct
    25921 aagattaaga aaagtgctac tgattggata aaagaaaatt tagaagctat gggcggtggc
    25981 gatttagtcg gcggaatatt agaccctgac aaaattaatt atcattatgg acgtaccgca
    26041 gcttataccg ctgcaactgg aagaccattt catgaaggtg tcgattttcc atttgtatat
    26101 caagaagtta gaacgccgat gggtggcaga cttacaagaa tgccatttat gtctggtggt
    26161 tatggtaatt atgtaaaaat tactagtggc gttatcgata tgctatttgc gcatttgaaa
    26221 aactttagca aatcaccacc tagtggcacg atggtaaagc ccggtgatgt tgttggttta
    26281 actggtaata ccggatttag tacaggacca catttacatt ttgaaatgag gagaaatgga
    26341 cgacattttg accctgaacc atatttaagg aatgctaaga aaaaaggaag attatcaata
    26401 ggtggtggcg gtgctacttc tggaagtggc gcaacttatg ccagtcgagt aatccgacaa
    26461 gcgcaaagta ttttaggtgg tcgttataaa ggtaagtgga ttcatgacca aatgatgcgc
    26521 gttgcaaaac gtgaaagtaa ctaccagtca aatgcagtga ataactggga tataaatgct
    26581 caaagaggag acccatcaag aggattattc caaatcatcg gctcaacttt tagagcaaac
    26641 gctaaacgtg gatatactaa ctttaataat ccggttcatc aaggtatttc agcaatgcag
    26701 tacattgtta gacgctacgg ttgggatgga tttaagcgtg cgggtgatta cgcatatgct
    26761 acaggcggaa aagtttttga tggttggtat aacttaggtg aagacggtca tccagaatgg
    26821 gttattccaa cagatccagc tcgtagaaat gatgcaatga agatgttaca ttatgcagct
    26881 gcggaagtta gagggaaaaa cgcaagtaag aataaacgac ctagtcaatt atctagtgta
    26941 aatgggtttg atgacccaag cttattattg aaaatgattg aacaacagca acaacaaata
    27001 gctttattac tgaagatagc gcaatctaac gatgtgattg cagataaaga ttatcagccg
    27061 attattgacg aatacgcttt tgataaaaag gtgaacgcgt ctatagaaaa gcgagaaagg
    27121 caagaatcaa caaaagtaaa gtttagaaaa ggaggaattg ctattcaatg atagacacta
    27181 ttaaagtgaa caacaaaaca attccttggt tgtatgtcga aagagggttt gaaataccct
    27241 cttttaatta tgttttaaaa acagaaaatg tagatggacg ttcggggtct atatataaag
    27301 ggcgtaggct tgaatcttat agttttgata tacctttggt ggtacgtaat gactatttat
    27361 ctcacaacgg cattaaaaca catgatgacg tcttgaatga attagtaaag ttttttaact
    27421 acgaggaaca agttaaatta caattcaaat ctaaagattg gtactggaac gcttattttg
    27481 aaggaccaat aaagctgcac aaagaatttg caatacctgt taagttcact atcaaagtag
    27541 tactaacaga cccttacaaa tattcagtaa caggaaataa aaatactgcg atttcagacc
    27601 aagtttcagt tgtaaatagt gggactgctg acactccttt aattgttgaa gcccgagcaa
    27661 ttaaaccatc tagttacttt atgattacta aaaatgatga agattatttt atggttggtg
    27721 atgatgaggt aaccaaagaa gttaaggatt acatgcctcc tgtttatcat agtgagtttc
    27781 gtgatttcaa aggttggact aagatgatta ctgaagatat tccaagtaat gacttaggtg
    27841 gtaaggtcgg cggtgacttt gtgatatcca atcttggcga aggatataaa gcaactaatt
    27901 ttcctgatgc aaaaggttgg gttggtgctg gcacgaaacg agggctccct aaagcgatga
    27961 cagattttca aattacctat aaatgtattg ttgaacaaaa aggtaaaggt gccggaagaa
    28021 cagcacaaca tatttatgat agtgatggta agttacttgc ttctattggt tatgaaaata
    28081 aatatcatga tagaaaaata ggacatattg ttgttacgtt gtataaccaa aaaggagacc
    28141 ccaaaaagat atacgactat cagaataaac cgataatgta taacttggac agaatcgttg
    28201 tttatatgcg gctcagaaga gtaggtaata aattttctat taaaacttgg aaatttgatc
    28261 acattaaaga cccagataga cgtaaaccta ttgatatgga tgagaaagag tggatagatg
    28321 gcggtaagtt ttatcagcgt ccagcttcta tcatagctat ctatagtgcg aagtataacg
    28381 gttataagtg gatggagatg aatggattag gttcattcaa tacggagatt ctaccgaaac
    28441 cgaaaggcgc aagggatgtc attatacaaa aaggtgattt agtgaaaata gatatgcaag
    28501 caaaaagtgt tgtcatcaat gaggaaccaa tgttgagcga gaaatcgttt ggaagtaatt
    28561 atttcaatgt tgattctggg tacagtgaat taatcataca acctgaaaac gtctttgata
    28621 cgacggttaa atggcaagat agatatttat agaaaggaga tgagagtgtg atacatgttt
    28681 tagattttaa cgacaagatt atagatttcc tttctactga tgacccttcc ttagttagag
    28741 cgattcataa acgtaatgtt aatgacaatt cagaaatgct tgaactgctc atatcatcag
    28801 aaagagctga aaagttccgt gaacgacatc gtgttattat aagggattca aacaaacaat
    28861 ggcgtgaatt tattattaac tgggttcaag atacgatgga cggctacaca gagatagaat
    28921 gtatagcgtc ttatcttgct gatataacaa cagctaaacc gtatgcacca ggcaaatttg
    28981 agaaaaagac aacttcagaa gcattgaaag atgtgttgag cgatacaggt tgggaagttt
    29041 ctgaacaaac cgaatacgat ggcttacgta ctacgtcatg gacttcttat caaactagat
    29101 atgaagtttt aaagcaatta tgtacaacct ataaaatggt attggatttt tatatagagc
    29161 ttagttctaa taccgtcaaa ggtagatatg tggtactcaa aaagaaaaac agcttattca
    29221 aaggtaaaga aattgagtat ggtaaagatt tggttgggtt aactaggaag attgatatgt
    29281 cagaaatcaa aacagcatta attgctgtgg gacctgaaaa tgacaaaggg aagcgtttag
    29341 agctagttgt gacagatgac gaagcgcaaa gtcaattcaa cctacctatg cgctatattt
    29401 gggggatata tgaaccacaa tcagatgatc aaaatatgaa tgaaacacga ttaagttctt
    29461 tagccaaaac agagttaaat aaacgtaagt cggcagttat gtcatatgag attacttcta
    29521 ctgatttgga agttacgtat ccgcacgaga ttatatcaat tggcgataca gtcagagtaa
    29581 aacatagaga ttttaacccg ccattgtatg tagaggcaga agttattgct gaagaatata
    29641 acataatttc agaaaatagc acatatacat tcggtcaacc taaagagttc aaagaatcag
    29701 aattacgaga agagtttaac aagcgattaa acctaataca ccaaaaatta aacgacaata
    29761 ttagcaatat caacactata gttaaagatg ttgtagatgg tgaattagaa tactttgaac
    29821 gcaaaataca caaaagtgat acaccgccag aaaatccagt caatgatatg ctttggtatg
    29881 atacaagtaa ccctgatgtt gctgtcttgc gtagatattg gaatggtcga tggattgaag
    29941 caacaccaaa tgatgttgaa aaattaggtg gtataacaag agagaaagcg ctattcagtg
    30001 aattaaacaa tatttttatt aatttatcta tacaacacgc tagtcttttg tcagaagcta
    30061 cagaattact gaatagcgag tacttagtag ataatgattt gaaagcggac ttacaagcaa
    30121 gtttagacgc tgtgattgat gtttataatc aaattaaaaa taatttagaa tctatgacac
    30181 ccgaaactgc aacgattggt cggttggtag atacacaagc tttatttctt gagtatagaa
    30241 agaaattaca agatgtttat acagatgtag aagatgtcaa aatcgccatt tcagatagat
    30301 ttaaattatt acagtcacaa tacactgatg aaaaatataa agaagcgttg gaaataatag
    30361 caacaaaatt tggtttaacg gtgaatgaag atttgcagtt agtcggagaa cctaatgttg
    30421 ttaaatcagc tattgaagca gctagagaat ccacaaaaga acaattacgt gactatgtaa
    30481 aaacatcgga ctataaaaca gacaaagacg gtattgttga acgtttagat actgctgaag
    30541 ctgagagaac gactttaaaa ggtgaaatca aagataaagt tacgttaaac gaatatcgaa
    30601 acggattgga agaacaaaaa caatatactg atgaccagtt aagtgatttg tccaataatc
    30661 ctgagattaa agcaagtatt gaacaagcaa atcaagaagc gcaagaagct ttaaaatcat
    30721 acattgatgc tcaagatgat cttaaagaga aggaatcgca agcgtatgct gatggtaaaa
    30781 tttcggaaga agagcaacgc gctatacaag atgctcaagc taaacttgaa gaggcaaaac
    30841 aaaacgcaga actaaaggct agaaacgctg aaaagaaagc taatgcttat acagacaaca
    30901 aggtcaaaga aagcacagat gcacagagga aaacattgac tcgctatggt tctcaaatta
    30961 tacaaaatgg taaggaaatc aaattaagaa ctactaaaga agagtttaat gcttctaaaa
    31021 gaacactatc aagattgtta gcagacatca ctgtaaatgc tatgaaaggc atctatttaa
    31081 ggtatgacga aaatggggcg attacttcac atactattga taaagatggc gtgaaaatta
    31141 gtggcgataa agttgatata acagcgaata gagaatttaa tgtagtcgca aataatatta
    31201 ataacaaagt tggtaaaaat gacattgtta atagcctaaa cttatcaaat gaaggtcttg
    31261 acatcaatgt gaatagaatt ggtattaaag gcggaaatgc taaccgttat gtacaagttc
    31321 aaaatgattt tattgaactt ggcggaatcg tacaacgaac ttggaaaggc aaacgatcaa
    31381 ccgatgatat attcacacgt cttaaagatg gacatctaag gtttagaaat aataccgcag
    31441 gcggttcact ttatatgtca cattttggta tttcaacata tattgatgga gaaggcgaag
    31501 acggaggttc atccggtact attcaatggt gggataaaac ttacagtgat agtggtatga
    31561 atggcataac aatcaattct tatggcggtg tagtcgcttt aacatctgac tacaatcgaa
    31621 ttattatcga ttcatatgct tcagctaata ttgaaagtag agaagcgccg atatatttat
    31681 ctccgaacac caaaaataaa cctggtttaa accgattcgc attcacatta tcaaacgctg
    31741 atagtgcata cgaaactgac ggttatatca tgtttggttc agatgaaaac tataagtacg
    31801 gtgctggatt aagattttct aaacgtagca ataaaggatt ggttcaagtc gttaatggtg
    31861 actatgctac aggcggagac actacaattg aatcaggtat gggcaaattc aacttagtta
    31921 aacgaagaga tggaaatagt tacgttagca ttcaaagtta tgatttattg gcggtaggtt
    31981 ctgataatgc tggcgataga gtcgcttcta attctattta taagcgtact tattcagcac
    32041 ctgctaactt acacattact tctgctggaa caattgggcg tgctacttct gccaaaaagt
    32101 ataaaatttc aatcgaaaac caatacatca atgaagacga tcagttcagt cattcaaaag
    32161 agattttaaa gcttccaatt cgtacatggt ttgacaaata tgaatcggaa ataatggcta
    32221 aagaattgga aagtggtaaa aagttatctg atgatacttt taaacttagt cgacatactg
    32281 gcttaatagc ggaagaggtt gaagaattag gatttaatga atttgttatt tatgatgaca
    32341 acggagaaat cgaaggtatc gcatacgata gactttggat tcatttaata cctattatta
    32401 aaaaccagca atcaaaaatc gaaaaactgg aggaattaat aaatgaatga tagcaatcaa
    32461 ggtttacaag ccaatccaca atatacaatt cactatttat cgcaagaaat cacaagacta
    32521 acacaagaaa atgcaatgtt aaaagcatat atacaagaac aaaatgaaaa aagcaaaagt
    32581 gctgaggaag agtaatcctt ggcactattt ttatacaaaa atttaaggag gtcatttaat
    32641 atggcaaatg aaattatcaa aaaaacagag agatttattt tagtacaaat tgacaaagag
    32701 ggaacagagc gcgttttgta tcaagatttt gtaggcagtt ttacaacgtc cgattcagca
    32761 agttatgcac aagattttaa atctgaggaa aacgctaaaa agattgctga aactttaaat
    32821 cttttatatc aattaacagg caatcaaaac ggtgtgaaag ttgtgaaaga agttgtggat
    32881 agaactgact tgtcatctga taaatcagtt gatagcgaaa caatgtaact atactaagtt
    32941 atgagcatta cgctcatagc tttcttagaa agtaggtgta gttttggatg atattcagaa
    33001 aataaaaaaa gagctttctg aattagttga acgtgttgat gatgttgaaa tactagcaaa
    33061 cgaaacagct gatcatgtgc ttgaacttag agaggaacat aagcaacatc ataatgaact
    33121 aagagaatct cataaagaac ttaaagataa gcaagataaa gttgtagatg agaatttaga
    33181 gcaaacaaag atattaaaca gaattgaaga aagatatcaa acgcaagtag atgttgcgca
    33241 aaaaaatgaa gaaaagacac tcgcccaaaa taaatggctc gtaggtgcca tatgggcgct
    33301 tgtaacaatt gttatgattg cagtcattac tgcatcaatt actgcgttat taccttaagg
    33361 gaggtggaca taatgagttg ggcaagatgg ttatcatgtt atttgtatgg tcgtaaatgt
    33421 aaataatgtt tttggtcagt gcatcggcac tggcttttta ttttgattga aaagaggtac
    33481 gtacatggta ttacacagct caaaagacag gaagcatact gcaagtgaag ttgggaagtg
    33541 ttgtcaatac caagtaagta agatatctga aatgtataat agagtaaaaa tgaaatcttt
    33601 ttattatatt atagacaagt ataaaaaagg tatagtaata tatgtatgta taagtaaata
    33661 atgatcattc cataattatt gtatataact aataattact ccaacaaaaa taatctatta
    33721 tacaaaaatt tcggataata acaagtttat atggaattat gctttagagg tgagtaaaat
    33781 aaaaaaaaca acatttatac tactttcatt tattgcccta acgttgataa caagtccatt
    33841 tgtaaattgt agcgagaaaa gcgaagaaat aaatggaaaa gatttacaaa aaaagtctga
    33901 attgcaggga actgctttaa gcaatcttag acaaacctat tatcataatg gaagtgctat
    33961 aattgaaaat aaagagagta atgatcaatt tttaaagaac actatattgt ttaatgattt
    34021 ttttacaggt catcaatggt ataatgattt attagtagac cttggttcaa aagacaccgc
    34081 caatatatac aaagggaaaa aagtggattt atatggtgtt tattatggtt atcaatgtac
    34141 tgggggtaca ccattcaaaa cagcttgtat gtatggcggt gtaacgttac atgataataa
    34201 tcaattggag gaagaaaaga aagtaccgat taacttatgg atagatggta aacaaaatac
    34261 agtacctttg ggaacggtta aaactaataa aaaagaagta actgttcagg agctagacct
    34321 tcagtcaaga cattatttac atgaaacata taatttatat aacacagatg catttaatgg
    34381 aaaaatacaa agaggattaa ttgagtttca cccttcttct ggtgattcgg ttggttatga
    34441 tttatttggt gctcaaggac aatatccaga tacacagttg aggatatata gagataataa
    34501 aacgattaag tctaaaaata tgcatattga tatatattta tatacaactt gagtgaaatt
    34561 ttggctgaaa aaatttaaaa tctataaaaa gtaccataag ttattttatt actatactta
    34621 aaacattcac ttttttactg ctgtttgtaa gttttataaa aaatttctat agatagtttt
    34681 gaacaaagcg agaataatct gaaagtatac aagcagtaaa aaaagtatat gtgctataat
    34741 atgctttgag caagttggat agatggtggc tatctgagta taaggaggtg gtgcctatgg
    34801 tggcattact gaaatcttta gaaaggagac gcctaatgat tacaattagt accatgttgc
    34861 agtttggttt attccttatt gcattgatag gtctagtaat caagcttatt gaattaagca
    34921 ataaaaaata accatcgcta actttggctg gtttcgatgg ttaaatggtt attaatttaa
    34981 tctttaatct aaaatagcca ccgtcttttt aacgggctca ctagggtaac atgtttgcgc
    35041 atgttgccct ttttctatat ataaattaac acaccataat ataaatatca aatagacggc
    35101 ttattagtcg tctttttatt ttggataaaa ggagataaga atatgattaa ttggaaaatt
    35161 agaatgaaac aaaaatcatt ttgggtagcg atattgtcag ctatcttttt atttgctcaa
    35221 aacatcgcca aagctattgg gtatgatatt caagtttata cagagcaatt aacagacggt
    35281 ttaaacgcta tattaggatt tttagtatta actggtgtga ttcaagaccc gactactaaa
    35341 ggtataggtg atagccacca agctttagaa tatgaagaac caagaagaaa atactaggag
    35401 gtaaaataat gaaaacatac agtgaagcaa gagcaaggtt acgttggtat caaggtagat
    35461 atattgattt tgacggttgg tatggttacc aatgtgcaga tttagcagtt gattacattt
    35521 attggttgtt agaaattaga atgtggggaa atgcaaaaga tgcaatcaat aacgatttta
    35581 aaaacatggc aacagtatat gaaaacacac catcgtttgt tccacaaata ggtgatgtgg
    35641 ctgtatttac caaaggaata tataaacaat acggtcatat tggtttagtg tttaatggtg
    35701 gtaatacaaa tcaattttta attttggaac aaaactatga cggtaacgca aatacgcctg
    35761 caaagttacg ttgggataat tattacggct gtactcactt tattagacct aagtataaaa
    35821 gtgagggctt aatgaataag atcacaaata aaattaaccc acctgctcaa aaagcagtcg
    35881 gtaaatctgc aagtaaaata acagttggaa gtaaagcacc ttataacctt aaatggtcaa
    35941 aaggtgctta ttttaatgcg aaaatcgacg gcttaggtgc tacttcagcc actagatacg
    36001 gtgataatcg tactaactat agattcgatg ttggacaggc tgtatacgcg cctggaacat
    36061 taatatatgt gtttgaaatt atagatggtt ggtgtcgcat ttattggaac aatcataatg
    36121 agtggatatg gcatgagaga ttgattgtga aagaagcatt ttaattctta ggttaaaatg
    36181 ttaaatattt gttaattatt ttttaatgta agtttagttt attttaatat tttcttgatt
    36241 tttaatattt tctcaatata aaatgaagtt gttgatattt atcatcttaa ataagtgtgt
    36301 tagctataaa aagagatgaa taaaaacaaa tatattatat ttggaggaag cgccatgctc
    36361 aaaagaagtt tattattttt aactgtttta ttgttattat tctcattttc ttcaattact
    36421 aatgaggtaa gtgcatcaag ttcattcgac aaaggaaaat ataaaaaagg cgatgacgcg
    36481 agttattttg aaccaacagg cccgtatttg atggtaaatg tggctggagt tgatagtaaa
    36541 ggaaatgaat tgctatcccc tcattatgtc gagtttccta ttaaacctgg gactacactt
    36601 acaaaagaaa aaattgaata ctatgtcgaa tgggcattag atgcgacagc atataaagag
    36661 tttagagtag ttgaattaga tccaagcgca aagatcgaag tcacttatta tgataagaat
    36721 aagaaaaaag aagaaacgaa gtctttccct ataacagaaa aaggttttgt tgtcccagat
    36781 ttatcagagc atattaaaaa ccctggattc aacttaatta caaaggttgt tatagaaaag
    36841 aaataaaaca aaatagttgt ttattataga aagcaatgtc ttgattgaat atgtgtagtg
    36901 aaaattatct ttcatcaaat tctcattcat gcacgaatgg ttcttcccca cctaatcaga
    36961 tattaggtga cttatgggga gaaatcagtt aggatgaaaa agtggataat ccttttttag
    37021 gcaggtactt cggtacttgc ctattttttt atgttataat ctttctagac gtattcaaag
    37081 gacgtctttt tagattgtat gttatagcta gccttccggt taattttttg ttatgatgtg
    37141 ttacacatgc atcaactatt tacatccttg ttcacccaag catgtcactg ggtgtttttt
    37201 cttatgatag agagcatagt tttcatacta ctcccccgta gtatatatga ctttagcatt
    37261 cccgtataat agtttacggg gtgcttttta tgttataatt aattgtatat agtaggagtg
    37321 aactatatag cctgttaagt ggcctagtaa cctaacactt atcctggcaa ttgataccct
    37381 ttttgccctt cactcgataa atatatctca acaacataga aatattacag tcgctacacc
    37441 gcatcttaaa tggtgtggtt atttttattg gaagtgtgta tcaggtatca gtaatgttaa
    37501 aacaccagct aaaaatgaaa agaattcacc agtgccagca ggttatacac tcgataaaaa
    37561 caatgaccgt ataaaaaaga gactggttat tacacagttg ccaatgttaa aggtaataac
    37621 gtgagggatg gctattcaac taattcaaga attacaggtg tattacccaa taacgcaacg
    37681 atcaaatatg acggcgcata ttgcattaat ggctatagat ggattactta tattgctaat
    37741 agtggacaac gtcgttatat agcgacagga gaggtagaca aggcaggtaa tagaataagc
    37801 ggttttggta agtttagtgc agtttgataa ttagatatat aaaggtttgg caagttatga
    37861 aatgtctgcc aaacctttat ataaaaaaga aatatctacc ttttaattta tgtaactact
    37921 attagtatgc atattcatta gttttaccag gaccattaat tacataagat gatttagact
    37981 ctcctttttt aaagaagtat gttttataca ttttacctag taactcaaca ttttttctat
    38041 cttcagcaag tggtgtattc agatatactg tatagtaacc tttattttca gttaaaataa
    38101 ccattttt
//
LOCUS       DAHHSC010000006        36064 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.006, whole genome shotgun sequence.
ACCESSION   DAHHSC010000006 DAHHSC010000000
VERSION     DAHHSC010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 36064)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36064)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36064
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.006"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            76..1395
                     /locus_tag="NBI57_000258"
     CDS             76..1395
                     /locus_tag="NBI57_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001622764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element IS1181 family transposase"
                     /protein_id="HCD1821245.1"
                     /translation="MCNDTLELLRIKDENIKYINQEIDVIIKGKKATVVNAVLTYKPS
                     ACYCCGVKNEGQIHKHGKRVSRITLLKTQGYNTYLNLAKQRFKCLECNGTFTAKTSIV
                     DESCFISRCVTQKVIEEATKVKTEIDTAEDNCISPSTVSRIRTKAANSLRIKPFNCLP
                     EHIAMDEFKSVKNVTGSMSFIFIDNDTHDVIDILENRTTRFLRAYFERFDLKNRQQVK
                     TVTIDMYEPYVRLFRDLFPNAAIIFDRFHIVQHLNRELNKYRVQVMNEYRNKKGPNYT
                     IFKNNWKVLLMDTSKTIFSKYRWNKSFKAYKRSSDIVEFMLSKDDILRRSYELVQGLR
                     KDLRLCNWPKFINRLNSVSKKSVSKGVWKVVKYYRKHQRMLRNTIYYPAFNNGAIEGI
                     NNKIKLIKRISFGYRNFNNFKARIMMIFSLYKGEKKKTTKPNNGLAA"
     gene            complement(1504..2553)
                     /locus_tag="NBI57_000259"
     CDS             complement(1504..2553)
                     /locus_tag="NBI57_000259"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HCD1821246.1"
                     /translation="MSDFQREQRKNEHVEIAMAQSDAMHSDFDKMRFVHHSIPSINVN
                     DIDLTSQTPDLTMAYPIYINAMTGGSEWTKNINEKLAVVARETGLAMAVGSTHAALRN
                     PRMAETFTIARKMNPEGMIFSNVGADVPVEKALEAVELLEAQALQIHVNSPQELVMPE
                     GNREFVTWLDNIASIVSRVSVPVIIKEVGFGMSKELMHDLQQIGVKYVDVSGKGGTNF
                     VDIENERRANKDMDYLSSWGQSTVESLLETTAYQSEISVFASGGLRTPLDAIKSLALG
                     AKATGMSRPFLNQVENNGIAHTVAYVESFIEHMKSIMTMLDAKNIDDLTQKQIVFSPE
                     IMSWIEQRSLNIHRG"
     gene            complement(2584..3531)
                     /gene="corA"
                     /locus_tag="NBI57_000260"
     CDS             complement(2584..3531)
                     /gene="corA"
                     /locus_tag="NBI57_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HCD1821247.1"
                     /translation="MALKIRYQTTYEPFKVVDDIKEIPKDATIVWYDFDEPNEQENEW
                     FKAHFNFNDLEVDDAINGMPRAKYKSYKDYQYLVFHSIMGSNYSPIALNIFIQDNVLV
                     TYHHQTLESLNKVVYKYMNTLDAELDCADVVILILDMMVDKYFNFVYALEDSVYHFED
                     RHVDDRFNKMVMDSVFKLRSDLIKVKRVLFPMQELIDTMKQNGDLIIDNKHSLYIQHI
                     DDHLIKQRNIIRTAQEMTNEIRENFESYTSFRMNSIMQVLTLVSVIFSPLTFIAGIYG
                     MNFVNMPALHLHYGYYICLAVMFVIAVVLIIFFRRKKWF"
     gene            3628..4119
                     /locus_tag="NBI57_000261"
     CDS             3628..4119
                     /locus_tag="NBI57_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HCD1821248.1"
                     /translation="MVIIKQLFTHTQTVTSEFIDHNNHMHDANYNIIFSDVVNRFNYS
                     HGLSLKERENLAYTLFTLEEHTTYLSELSLGDVFTVTLYIYDYDYKRLHLFLTLTKED
                     GTLASTNEVMMMGINQHTRRSDAFPESFSTQIAHYYKNQPTITWPEQLGHKIAIPHKG
                     ALK"
     gene            4116..4535
                     /locus_tag="NBI57_000262"
     CDS             4116..4535
                     /locus_tag="NBI57_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1821249.1"
                     /translation="MTDALQQKIHIELLDLLDDVKFELTELNAQKGLYINGPANQLLK
                     RGVHMAYVQGQKQAIDNIMTIVEQQLEDQHFLEDYDKFQNEVTHRNYDKTANFAELSD
                     IPRQFDNFLDQFYQIKGQYFIITHINTLIGDFHSEAH"
     gene            complement(4643..5563)
                     /locus_tag="NBI57_000263"
     CDS             complement(4643..5563)
                     /locus_tag="NBI57_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000620861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCD1821250.1"
                     /translation="MIRNRVMNSVVNKYLLHNRSIMFKNDQDVERFFYKREIENRKKH
                     KQPSTLNVKANLEKLSLDDMQVFRFNFRHQIDKKILYIHGGFNALQPSPFHWRLLDKI
                     TLSTLYEVVLPIYPKTPEFHIDDTFQAIQRVYDQLVSEVGHQNVVVMGDGSGGALALS
                     FVQSLLDNQQPLPNKLYLISPILDATLSNKDISDALIEQDAVLSQFGVNEIMKKWANG
                     LPLTDKRISPINGTIEGLPPVYMFGGGREMTHPDMKLFEQMMLQHHQYIEFYDYPKMV
                     HDFPIYPIRQSHKAIKQIAKSIDEDVTQNN"
     gene            5771..6409
                     /locus_tag="NBI57_000264"
     CDS             5771..6409
                     /locus_tag="NBI57_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1821251.1"
                     /translation="MTNPNQRLEPFDETFQQPNIHRGKRYGKKKRSLVSMIIQIIVVI
                     LTAIAGYSMWKQPIFNIVFTKESVDFDGIKNFNETIGKMHQLNINLGNINDLQQGIDR
                     LILVFNGFFILCIVSLVITILTIVFNRTVLKVVNILVLTIMLVITLYFSYIIKTLAQE
                     IADALKKYYLNVPPDQVITEADAIHNGIILLGCSIGLLVISLFFRNRMPRLK"
     gene            complement(6655..8586)
                     /locus_tag="NBI57_000265"
     CDS             complement(6655..8586)
                     /locus_tag="NBI57_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HCD1821252.1"
                     /translation="MTTTFIISYIILALIIVGVINLFLIRSRKKGKRQQKEQQFTTRQ
                     SNQSKFKASDLDKTTDQSTQRMTHEELRVDNQDDHSQVSLNGYTKGSEKDQEAFTNNN
                     GEEAVAAKNPESEEYKVNEKIKKEHKNFIFGEGVSRGKILAALLFGMFIAILNQTLLN
                     VALPKINTEFNISASTGQWLMTGFMLVNGILIPITAYLFNKYSYRKLFLVALVLFTIG
                     SLICAISMNFPIMMVGRVLQAIGAGVLMPLGSIVIITIYPPEKRGAAMGTMGIAMILA
                     PAIGPTLSGYIVQNYHWNVMFYGMFIIGIIAILVGFVWFKLYQYTTNPKADIPGIIFS
                     TIGFGALLYGFSEAGNKGWGSVEIETMFAIGIIFIILFVIRELRMKSPMLNLEVLKFP
                     TFTLTTIINMVVMLSLYGGMILLPIYLQNLRGFSALDSGLLLLPGSLIMGLLGPFAGK
                     LLDTIGLKPLAIFGIAVMTYATWELTKLNMDTPYMTIMGIYVLRSFGMAFIMMPMVTA
                     AINALPGRLASHGNAFLNTMRQLAGSIGTAILVTVMTTQTTQHLSAFGEELDKTNPVV
                     QDHMRELASQYGGQEGAMKVLLQFVNKLATVEGINDAFIVATIFSIIALILCLFLQSN
                     KKAKATAQKLEASSNNNHE"
     gene            complement(8599..9246)
                     /locus_tag="NBI57_000266"
     CDS             complement(8599..9246)
                     /locus_tag="NBI57_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HCD1821253.1"
                     /translation="MKKMILINVITVVVLLAIGIAGFYFWNKTTSYVTTDNAKVNGDQ
                     IKIASPASGQIKSLNVKQGDKLDKGDKVATVTVQGQDGETKDMDLKMPQKGTIAKLDG
                     MEGSMVQAGNPIAYAYNLDDLYVTANIDEKDIKDVEVGKDVDVTIDGQKASIKGKVDS
                     IGKATAASFSLMPSSNSDGNYTKVSQVIPVKITLESEPSKQVVPGMNAEVKIHKN"
     gene            9367..9921
                     /locus_tag="NBI57_000267"
     CDS             9367..9921
                     /locus_tag="NBI57_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001661235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCD1821254.1"
                     /translation="MKRQAKIEIQNALVDLMAEYPFQEISTKMICAYCNINRSTFYDY
                     YKDKFDLLDTINSKHKEKFQFLLSALHHNFENIKQDKLKLYKFFIIIAKYIKHNEQFF
                     KDILVTYPMKTLFIDYINLARDYYQQIMNDYSTTVTNKQLYVTYIIGGQAGVFINWLS
                     NGCNESPEEVADILLANTIKLQIQ"
     gene            complement(10148..11356)
                     /locus_tag="NBI57_000268"
     CDS             complement(10148..11356)
                     /locus_tag="NBI57_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000999124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug effflux MFS transporter"
                     /protein_id="HCD1821255.1"
                     /translation="MNDLTHFNKRSPIFIIILGSLTAIGALSIDMFLPGLSDIRHDFQ
                     TTTSNAQLTLSMFMIGLAFGNLFAGPISDSTGRRKPLIIAMIIFTLASLGIVFVHNIW
                     LMVALRFLQGVTGGAAAVISRAIASDMYSGNELTKFMALLMLVNGIAPVVAPTIGGII
                     LNYSVWRMVFVILTIFGFVMVIGSLLKVPESLTVTNRESSSGLKTMFKNFKILLKTPR
                     FVLPMLIQGMTFVILFTYISASPFIIQKIYGMTAIQFSWMFAGIGITLIISSQLTGYL
                     VDFIDSQKLMRGMTMIQIIGVILVTIVLLNHWNFWILAIGFIILIAPVTGVATLGFTI
                     AMDESSSGRGSSSSLLGLVQFLFGGVASPLVGVKGEDNPIPYIIIIIATAVILIILQI
                     YNMKVFKTNR"
     gene            complement(11466..11594)
                     /locus_tag="NBI57_000269"
     CDS             complement(11466..11594)
                     /locus_tag="NBI57_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1821256.1"
                     /translation="MEYKQHPFKVFTISVNYFEGIVFCEKIYGVLRFISFYTFYKL"
     gene            complement(11625..13007)
                     /locus_tag="NBI57_000270"
     CDS             complement(11625..13007)
                     /locus_tag="NBI57_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HCD1821257.1"
                     /translation="MKSCPKCGQQAQDDVQICTQCGHKFDSRQALYRKSTDEDIQTNN
                     IKMRKMVPWAIGFFILILIIILFFLLRNFNSPEAQTKILVNAIENNDKQKVATLLSTK
                     DNKVDSEEAKVYINYIKDEVGLKQFVSDLKNTVHKLNKSKTSVASYIQTRSGQNILRV
                     SKNGTRYIFFDNMSFTAPTKQPIVKPKEKTKYEFKSGGKKKMVIAEANKVTPIGNFIP
                     GTYRIPAMKSTENGDFAGYLKFDFRQSNSETVDVTEDFEEANITVTLKGDTKLNDSSK
                     KVTINDREMAFSSSKTYGPYPQNKDITISASGKAKGKTFTTQTKTIKASDLKYNTEIT
                     LNFDSEDIEDYVEKKEKEENSLKNKLIEFFAGYSLANNAAFNQSDFDFVSSYIKKGSS
                     FYDDVKKRVSKGSLMMISSPQIIDAEKHGDKITATVRLINENGKQVDKEYELEQGSQD
                     RLQLIKTSEK"
     gene            complement(13247..13702)
                     /locus_tag="NBI57_000271"
     CDS             complement(13247..13702)
                     /locus_tag="NBI57_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017804647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HCD1821258.1"
                     /translation="MVKHLQDHIQFLEQFINNVNALTAKMLKDLQNEYEISLEQSNVL
                     GMLNKEPLTISEITQRQGVNKAAVSRRIKKLIDAKLVKLDKPNLNIDQRLKFITLTDK
                     GRAYLKERNAIMTDIAQDITNDLNSEDIENVRQVLEVINHRIKTYSNHK"
     gene            13897..14865
                     /locus_tag="NBI57_000272"
     CDS             13897..14865
                     /locus_tag="NBI57_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HCD1821259.1"
                     /translation="MLLNLLLIFILIVICPLIFNYTLTLNLTCYILVVISIFLSLIYS
                     IMTYTFPWSMFVTFIIISIIMLFKHRYLFSHTSSRLFIKKLLSFSYKFVLYTSALLFI
                     STIQSPIVKGLSMWLAILCLSLLLTFICYLVWTVSYRHRSYNKEVDIIIVLGAGIFTE
                     FVTPMLAARLDRALDIYQQQASATKIIVSGGQGPDEPISEALAMQRYLIAHGVDKTDI
                     LMESHSINTYTNFLYSKQIINNLYHEAVKIVCVTSQFHVLRALRLAQKLGLSIDGVGS
                     HTPYHFFLHVLIKDYLGVMYQYRLLLTLYCSSSWFICLYAAFIYNS"
     gene            complement(15096..15761)
                     /locus_tag="NBI57_000273"
     CDS             complement(15096..15761)
                     /locus_tag="NBI57_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCD1821260.1"
                     /translation="MALVVKDIVKNFGEGLSETKVLKGINFEVEQGEFVILNGASGSG
                     KTTLLTILGGLLSQTSGTVLYNDAPLFDKQHRPSDLRLEDIGFIFQSSHLVPYLKVIE
                     QLTLVGQEAGMTKQQSSTRAIQLLKNIGLEDRLNVYPHQLSGGEKQRVAIMRAFMNNP
                     KIILADEPTASLDADRATKVVEMIRQQIKEQQMIGIMITHDRRLFEYADRVIELEDGK
                     ITD"
     gene            complement(15761..16816)
                     /locus_tag="NBI57_000274"
     CDS             complement(15761..16816)
                     /locus_tag="NBI57_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCD1821261.1"
                     /translation="MKLAIKEIMFYKFRYILITLIILLLSIMVLFISGLAQGLGRENI
                     SLFEHFDNDEYVVQKMKEPQIEKSQLSDTQQNQIKKVIHQEPYKMNIQTLKLSNKEQD
                     VITMNDVKQQRIQLKKGDYPKNAHEVAINDKLAADNIRVGDRLHFKNNSTSYRVSGIL
                     NDTMYAHSSIVLLNDNGFNALNKVNTAFYPVKNLTQQQRDELNKINDVQVVSEKDLTG
                     NIASYQAEQAPLNMMIVSLFAITAIVLSAFFYVMTIQKISQIGILKAIGIKTRHLLSA
                     LVLQILTLTIIGVGIAVIIIVGLSFMMPVTMPFYLTTQNILLMVGIFILVAILGASLS
                     FIKLFKVDPIEAIGGAE"
     gene            16952..17626
                     /gene="hssR"
                     /locus_tag="NBI57_000275"
     CDS             16952..17626
                     /gene="hssR"
                     /locus_tag="NBI57_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding heme response regulator HssR"
                     /protein_id="HCD1821262.1"
                     /translation="MVQCLVVDDDPRILNYIASHLQTEHIDAYTQPSGEAALKLLEKQ
                     RVDIAVVDIMMDGMDGFQLCNTLKNDYDIPVIMLTARDALSDKERAFISGTDDYVTKP
                     FEVKELIFRIRAVLRRYNINSNSEMTIGNLTLNQSYLELQVSNKTMTLPNKEFQLLFM
                     LAARPKQIFTREQIIEKIWGYDYEGDERTVDVHIKRLRQRLKKLNATLTIETVRGQGY
                     KVENHV"
     gene            17619..18992
                     /gene="hssS"
                     /locus_tag="NBI57_000276"
     CDS             17619..18992
                     /gene="hssS"
                     /locus_tag="NBI57_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000477337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme sensor histidine kinase HssS"
                     /protein_id="HCD1821263.1"
                     /translation="MFKTLYARIAIYSITVILFSALISFVLTNVYYHYNLKASNDAKI
                     MKTLKEARQYEQLAKPTHIQQYFKHLGQMNYQIMTVDQKGHKTFYGEPFREDTLSQNA
                     INNVLNNKDYHGIKDKPFALFVTGFFDNVTDNTVGINFKTKDGSIAVFMRPDIGETFS
                     EFRTFLAVLLMLLLFISISLVIASTYSIIRPVKKLKLATERLIDGDFETPIKQTRKDE
                     IGTLQYHFNKMRESLGQVDQMRQHFVQNVSHEIKTPLTHIHHLLSELQQTSDKTLRQQ
                     YINDIYTITTQLSGLTTELLLLSELDNHQHLLFDDKIQVDQLIKDIIRHEQFAADEKS
                     LIILADLESINFLGNQRLLHQALSNLLINAIKYTDVGGAIDIALQHSHNNIIFTISND
                     GSPISPQAEARLFERFYKVSKHDNSNGLGLAITKSIIELHHGTIQFTQSNEYVTTFTI
                     TLPNNSH"
     gene            19155..19598
                     /locus_tag="NBI57_000277"
     CDS             19155..19598
                     /locus_tag="NBI57_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCD1821264.1"
                     /translation="MMKLNLFINANETESYIDIHAPKMNDHVQSIINAVNDLDKSHTL
                     VGYIDKEIHIINVSDVITFQVINKNVTAITSNQKFKLKLRLYELEKQLPQHFIRISKS
                     EIVNKYYIEKLLLEPNGLIRMYLKDAHYTYSSRRYLKSIKERLSI"
     gene            19595..20050
                     /locus_tag="NBI57_000278"
     CDS             19595..20050
                     /locus_tag="NBI57_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3021 domain-containing protein"
                     /protein_id="HCD1821265.1"
                     /translation="MKNLKNSLFISLIIGLSLSLFFSMLFADGKYYPLNPQSTIGILY
                     YNHFTETTVMLISIILWLLIGVVFFLGDFIFKYTDWSITKATIMHFITTYVGFLPLAM
                     LAGWFPLTVHYLIIFTIIFIVIYVLIWIIQFFKNKNYVDTINEQLKQLK"
     gene            complement(20116..21594)
                     /gene="mqo"
                     /locus_tag="NBI57_000279"
     CDS             complement(20116..21594)
                     /gene="mqo"
                     /locus_tag="NBI57_000279"
                     /EC_number="1.1.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase (quinone)"
                     /protein_id="HCD1821266.1"
                     /translation="MTTQHSKTDVILIGGGIMSATLGTLLKELSPEKNIKVFEKLAQP
                     GEESSNVWNNAGTGHSALCELNYTKEGKDGTVDCSKAIKINEQYQISKQFWAYLVKTG
                     QLDNPDRFIQAVPHMSFVIGEDNVAFIKSRVATLKKSVLFEKMKLSQDEEEMKSWVPL
                     MIEGRKSDEPIALTYDETGTDVNFGALTAKLFENLEQRGVGIQYKQNVLDIKKQKSGA
                     WLVKVKDLETNETTTYESDFVFIGAGGASLPLLQKTGIKQSKHIGGFPVSGLFLRCTN
                     QEVIDRHHAKVYGKAAVGAPPMSVPHLDTRFVDGKRSLLFGPFAGFSPKFLKTGSHMD
                     LIKSVKPNNIVTMLSAGIKEMSLTKYLVSQLMLSNDERMDDLRVFFPNAKNEDWEVIT
                     AGQRVQVIKDTEDSKGNLQFGTEVITSDDGTLAALLGASPGASTAVDIMFDVLQRCYR
                     DEFKGWEPKIKEMVPSFGYRLTDHEDLYHKINEEVTKYLQVK"
     gene            complement(21975..23573)
                     /locus_tag="NBI57_000280"
     CDS             complement(21975..23573)
                     /locus_tag="NBI57_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HCD1821267.1"
                     /translation="MLVNTFNPFDNLLLSSLIAAIPIVLFLLCLTVFKMKGIYAAITT
                     LVVTLLIAIPFFKLPVGIASGAVVEGFFQGIIPIGYIVMMAVLLYKITVESGQFLTIQ
                     DSITNISQDQRIQVLLIGFAFNAFLEGAAGFGVPIAICALLLTQLGFNPLKAAMLCLV
                     ANAASGAFGAIGIPVGVVETLKLPGDVSVLGVSQSATLTLAIINFIIPFLLIFIIDGF
                     RGVKETLPAILVVSITYTLTQGLLTVFSGPELADIIPPLLTMLALAVFSKKFQPKHIY
                     RVNKDEEIEPAKAHSAKAVLHAWSPFIVLTVIVMIWSAPFFKNLFLPNGALSSLVFKF
                     NLPGTISEVTHKPLVLTLNIIGQTGTAILLTIIITILMSKKVNFKDAVRLFGVTFKEL
                     WLPVLTICFILAISKITTYGGLSAAMGQGIAKAGNVFPVLSPILGWIGVFMTGSVVNN
                     NSLFAPIQASVAQQIGTSGSLLVSANTVGGVAAKLISPQSIAIATAAVKQVGKESELL
                     KMTLKYSVCLLIFICIWTFILSLL"
     gene            23890..25563
                     /locus_tag="NBI57_000281"
     CDS             23890..25563
                     /locus_tag="NBI57_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-glycerol glycerophosphotransferase family
                     protein"
                     /protein_id="HCD1821268.1"
                     /translation="MIKQINVSNMQKFESQLMKAQSEGYTHVVPYANEIMIYQSMLDA
                     VQLYPKSIVVDYTVDGQYKNDCHYFGQSSINIADWAQNNNYYPNLIYAIQQTLDLIHY
                     YSVETIFDLALLTLLKGDLSIDGHVVFDFKAPIATSASIWETIKTIEDFDMMSQFYLN
                     KMAYIDHHPIPFRNLFIEDSEQLNSPDNWLYSTKFMLPKWLYKIAKQRADNKQLQNFG
                     LYTKQPNVLKDHIVFIGDHHQYIGNSKYLFTYFVKHNPMTACYFVTDDRRGPHFISPK
                     SEKADELINSARVVIVENDIPETLQPNGTLIQLHQGTPIMQLFLDSKEPIKNIETPFY
                     RAKRYNRWLQFDYVIHSADDISHFYQTAFPSHQANVLAYGNPKHQYLLQKRNESTTQQ
                     QYKKSFKINDQKPVLLYAPIGLVSAQQLPLSDALFKAYHVVVQGVDEAILPEEALVAP
                     KYLSAQDLILMSDVVITDYSNIIFDAMAIDKTVALYTPNHSQYIESQGVNEDIWRHLS
                     KIWYTDRQLLINNLISQAIPVIKYPQIQHKEQPLESISQLILSKMTSNQ"
     gene            complement(25717..26331)
                     /locus_tag="NBI57_000282"
     CDS             complement(25717..26331)
                     /locus_tag="NBI57_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1821269.1"
                     /translation="MKRLVTGLLALSLFLAACGQDSDQQKDSNKEKDDKAKTEQQDKK
                     TNDSSKDKKDNKDDSKDVNKDNKDNSANDNQQQSNSNATNNDQNQTNNNQSSNNQKSS
                     YVAPYYGQNAAPVARQIYPFNGNKTQALQQLPNFQTALNAANNEANKFGSNNKVYNDY
                     SIEEHNGNYKYVFSFKDPNANGKYSIVTVDYTGQAMVTDPNYQQ"
     gene            complement(26645..27160)
                     /locus_tag="NBI57_000283"
     CDS             complement(26645..27160)
                     /locus_tag="NBI57_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCD1821270.1"
                     /translation="MAGIIKEISEQNASELVELATRTFYDTFGSYYDDKDFDQFFKDN
                     YTVEKFTQEINHVDSFHYFYQEDGANVGYIKMNINSAQTEEMGETYLEVQRIYFLKDF
                     QGGGRGSQLIELAEKIAQEHNKHKIWLGVWEHNPRAQAFYKRHGFKVVGEHHFQTGDV
                     TDTDLIMEKEL"
     gene            27351..28355
                     /locus_tag="NBI57_000284"
     CDS             27351..28355
                     /locus_tag="NBI57_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000573244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HCD1821271.1"
                     /translation="MIETFKAFVIDKDESGKVTPTFKQLSPTDLPKGDVLIKVHYSGI
                     NYKDALATQDHNAVVKSYPMIPGIDLAGTIVESEAPGFEKGEQVIVTSYDLGVSHYGG
                     FSEYARVKSEWIIKLPDTLTLEESMIYGTAGYTAGLAIERLEKVGMNIEDGPVLVRGA
                     SGGVGTLAVLMLNELGYKVIASTGKQDVSDQLLELGAKEVIDRLPVEDDHKKPLASST
                     WQACVDPVGGEGINYVTKRLNHSGSIAVIGMTAGNTYTNSVFPHILRGVNILGIDSVF
                     TAMKLRQRVWRRLAKDLKPENLHEIKQVITFDELPEQLNKVINHENKGRIVIDFGVDK
                     "
     gene            complement(28401..28802)
                     /locus_tag="NBI57_000285"
     CDS             complement(28401..28802)
                     /locus_tag="NBI57_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCD1821272.1"
                     /translation="MVKVTYDIPTCEDYCALRINAGMSPKTREAAEKGLPNALFTVTL
                     YDKDRLIGMGRVIGDGGTVFQIVDIAVSKSYQGQDYGSLIMEHIMKYIKNVSVESAYV
                     SLIADYPADKLYAKFGFMPTEPDSGGMYIKY"
     gene            complement(28838..29872)
                     /locus_tag="NBI57_000286"
     CDS             complement(28838..29872)
                     /locus_tag="NBI57_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HCD1821273.1"
                     /translation="MKDVTIIGGGPSGLYASFYAGLRDMSVRLIDVQSELGGKMRIYP
                     EKIIWDIGGIAPKPCHEILKDTIKQGLCFKPEVHLNERVVDIRKKAERHFEVETEAGE
                     IYTSKAVIIAIGAGIINPKQLDVKGVERYQLTNLHYVVQSYRRFKDKDVLISGGGNTA
                     LDWAHDIAKIAKSVTVVYRKEDVSGHEAMKTLVTDLNVKLCPKTRIKYLVGNDDETHI
                     SEVVLEHVESGDRHTVKFDDVIISHGFDRCNTLLSETSSKLDMHDDCRVKGFGNTTTS
                     IPGIYACGDIVYHDAKSHLIASAFSDGANAANLAKTYIQPDANAEGYVSSHHEVFKEA
                     NKTIVNKHLY"
     gene            complement(29883..29999)
                     /locus_tag="NBI57_000287"
     CDS             complement(29883..29999)
                     /locus_tag="NBI57_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1821274.1"
                     /translation="MINYAIYFNNININSIMALNDNNYQFEHVNERNEYRES"
     gene            complement(30104..30535)
                     /locus_tag="NBI57_000288"
     CDS             complement(30104..30535)
                     /locus_tag="NBI57_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2871 domain-containing protein"
                     /protein_id="HCD1821275.1"
                     /translation="MRRLLYSFLFYMIIGLFSGFFYRELTKAYDFTGTTQLSLVHTHT
                     LILGMFMFLILLPLEKLFKLSSYYLFNWFFYVYNIGVIVTIGMMVTKGFFQVTGKSYS
                     PEAFAGFAGIGHTGMLAGLLLLFFLLRQAILKEPRDESFKK"
     gene            complement(30742..32019)
                     /locus_tag="NBI57_000289"
     CDS             complement(30742..32019)
                     /locus_tag="NBI57_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
                     /protein_id="HCD1821276.1"
                     /translation="MNIFKNKLLWIAPIATMIILVIFSLAFYPAYNPKPKDLPIGILN
                     EDKGTTIQDKNVNIGKKLEDKLLDSDSNKIKWVKVDSEKDPEKDLKDQKIFGVAIIDK
                     DFSKDAMSKTQKVVMDSKKEEMQQKVASGEIPPQVVQQMKQKMGNQQVEVKQAKFKTI
                     VSEGSSLQGSQIASAVLTGMGDNINAQITKQSLETLTSQNVKVNAADINGLTNPVKVD
                     NEKLNKVKDHQAGGNAPFLMFMPIWIGSIVTSILLFFAFRTSNNIVVQHRIIASIGQM
                     IFAVVAAFAGSFVYIYFMQGVQGFDFDHPNRIAIFVAFAILGFVGLILGVMVWLGMKS
                     VPIFFILMFFSMQLVTLPKQMLPESYQKYVYDWNPFTHYATSVRELLYLNHHIELNST
                     MWMFIGFMIFGAVSSLVSAIVRKHSTKRTEVPS"
     gene            32180..32803
                     /locus_tag="NBI57_000290"
     CDS             32180..32803
                     /locus_tag="NBI57_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCD1821277.1"
                     /translation="MKEDRRIRKTKSSIKQAFTKLLQEKDLEKITIRDITTRADINRG
                     TFYLHYEDKYMLLADMEDEYISELTTYTQFDLLRGSSIEDIANTFVNNILKNIFQHIH
                     DNLEFYHTILQLERTSQLELKINEHIKNNMQRYISINHSIGGVPEMYFYSYVSGATIS
                     IIKYWVMDKQPISVDELAKHVHNIIFNGPLRIMAENRLHKSNLDSLT"
     gene            32849..33793
                     /locus_tag="NBI57_000291"
     CDS             32849..33793
                     /locus_tag="NBI57_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HCD1821278.1"
                     /translation="MITSFRHSEDIDKHIIKTPLDHTASWINVVEPDREEIENLMEQY
                     NIPEDFIRDPLDSEESSRIEYDEDTGYSLIIIDLPIVNSTNRSVLSFVTIPLGIIIGN
                     GIIVTVCDAENEFLENLPKRDINLKFHSRFALEILTTIADHYNRNLRLLNKSRIRIEK
                     ELKNNITNKQLFKLMEVEKSLVYFLAALKGNDTIIKKLFRLPAIKRFEEDEELLEDLI
                     IENNQAIETTELHQRILESITTSYASLLSNDMNTIMKTLTLFTVLLTLPTLVFSFFGM
                     NVPLPIDDHSYISWIIVVGISLILVVIVSIFLWRKQKL"
     gene            complement(33878..35320)
                     /locus_tag="NBI57_000292"
     CDS             complement(33878..35320)
                     /locus_tag="NBI57_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sucrose transporter subunit IIBC"
                     /protein_id="HCD1821279.1"
                     /translation="MNYKQSAEDILNAIGGEENLDAMAHCATRLRLVLNDESLVNEEA
                     LNNMDVVKGTFSTGGQYQIIIGSGTVNKVFSELEKLTGKEASTTSEVKAQSAKNMNPL
                     QRFVKMLSDIFVPIIPAIVAGGLLMGLNNILTAKDLFFSGKSLIDVYSQFAGLAEMIN
                     VFANAPFTLLPILIGFSAAKRFGGNPFLGAVLGMILVHPSLMSAYDFPKAVEAGKAIP
                     YWDVFGLHINQVGYQGQVLPMLVAAYILASIEKGLRKVIPTVLDNLLTPLLSIFITAF
                     LTFSFVGPITRQLGYWLSDGLTWLYEFGGAIGGLIFGLLYAPIVITGMHHSFIAVETT
                     LIADATKTGGSFIFPIATMSNVAQGGAAIAAFFIIKQNKKLKGVASAAGISALLGITE
                     PAMFGVNLKLRYPFIGAIVGSGIGSAYIAFFKVKAIALGTAGLPGFISINPVHAGWLH
                     YFVGMTISFIIAITVTLILSKRKANKEVVE"
     gene            35503..35886
                     /locus_tag="NBI57_000293"
     CDS             35503..35886
                     /locus_tag="NBI57_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgA family protein"
                     /protein_id="HCD1821280.1"
                     /translation="MKERGHIEQLWREEKYHVLLHSQQSYQMIRNALKTDLSLHQVQQ
                     MIDDALLIEPSIGSVCNAFDHMWGYFKKCANEEERKQSKLLKADFINGKIDTQTLLDF
                     LAELANKYDVQYLLQSRVLNTKRKR"
BASE COUNT        12146 a         6400 c         5097 g        12421 t
ORIGIN      
        1 tttaatacac aaaaagcgca attgcctcta taatttaaag tgaccaaacc caaactaaag
       61 gagacaagtg cgcctatgtg taatgatacc ttagaattac taagaataaa agatgaaaat
      121 ataaaatata taaaccaaga aattgacgtc attatcaaag gaaaaaaagc aacagtggtt
      181 aatgctgtac taacgtataa gccttcggcc tgttattgtt gtggagttaa aaatgaagga
      241 caaattcata aacatggtaa gcgtgtttct cgtattactt tacttaaaac tcaagggtat
      301 aacacatacc tcaacttagc taaacaacgt tttaaatgct tagaatgcaa tggcactttt
      361 actgctaaaa cgtcaattgt tgatgagtcg tgttttatct caagatgtgt tactcaaaaa
      421 gttatagaag aagctactaa agttaaaaca gagattgata ctgcagaaga taactgtatc
      481 tctccatcta ctgtaagtcg tattagaact aaagcggcta attcattacg aattaaaccg
      541 tttaattgtt tgccagaaca catcgctatg gatgaattta aaagcgttaa aaatgtaact
      601 ggatcaatga gtttcatttt tatagataat gatactcatg atgttataga tattttagaa
      661 aatagaacta caagattctt gcgtgcctat ttcgagcgat tcgatttaaa aaatcgacaa
      721 caagttaaga cggttactat tgacatgtat gaaccctatg tccgattatt tcgcgaccta
      781 tttcctaatg cagctattat ttttgacaga ttccatatcg ttcaacattt aaatagggaa
      841 cttaataagt atcgtgtaca agttatgaat gaataccgta ataaaaaagg acctaattat
      901 acaattttta agaataactg gaaagttcta ttgatggata ctagtaaaac catatttagt
      961 aaatacagat ggaataaatc ttttaaggct tataaacgct catctgacat tgtagaattc
     1021 atgctttcaa aagacgatat actacgacgc tcctacgaac ttgtccaagg attaagaaaa
     1081 gatctaaggt tatgtaattg gcctaaattt attaatcgtt taaattcagt tagtaaaaag
     1141 tctgtgagta aaggtgtatg gaaagtggtt aaatattata gaaaacatca aaggatgtta
     1201 agaaatacaa tttattaccc agcatttaat aatggtgcta tagaaggaat taataataag
     1261 ataaaattaa tcaagcgaat ttcttttggt tacagaaatt tcaacaactt taaagcacgt
     1321 ataatgatga ttttcagctt atacaaagga gaaaaaaaga agacaaccaa gcccaataat
     1381 ggactggccg cctaataata aaaactctaa aagttgtaat ttaaaatagt tctttaaatt
     1441 atatacccac cacatttggt ggagaaccta aataaaagag gaggcctccg cctcctcttt
     1501 tatttatcct cgatgtatat tcaagctacg ttgttctatc catgacatta tttccggact
     1561 aaatacgatt tgtttttgtg ttaagtcgtc aatattttta gcatctaaca tcgtcattat
     1621 tgatttcatg tgttcaataa atgattctac ataagctact gtatgtgcaa tgccattatt
     1681 ttcaacttga tttaaaaacg gacgtgacat accagttgcc tttgcaccaa gtgctaaact
     1741 tttaattgca tcgagtggtg tacgtaaacc accactcgcg aaaactgaaa tttcgctttg
     1801 ataagccgtt gtttcaagta atgactcaac tgtagactgt ccccatgatg ataagtaatc
     1861 catatcttta tttgcacgac gttcattttc aatatctaca aagttagtac cacctttgcc
     1921 actaacatcg acatacttga cgcctatttg ttgtaagtca tgcattaatt ctttgctcat
     1981 accaaatcca acttctttta taatgactgg aacagacact cgtgatacaa tcgacgctat
     2041 attatctaac caagtcacaa attcacgatt cccttcaggc ataactaatt cttgaggaga
     2101 attaacatgg atttgtaacg cttgtgcctc aagtaattca actgcttcca aagccttttc
     2161 tactggtacg tccgcaccaa cattgctaaa aatcatgcct tcaggattca tttttcgcgc
     2221 aatcgtaaac gtctcagcca tgcgtggatt tctcaatgcc gcatgtgttg atccaactgc
     2281 catcgctaag ccagtttctc ttgcaactac agctagcttt tcattgatgt ttttcgtcca
     2341 ctcgctacca cccgtcattg cattaatata aatcggatat gccatcgtta agtcaggcgt
     2401 ctgtgatgtc aaatcgatat catttacatt aattgatggg atagaatgat gcacaaaacg
     2461 catcttatca aaatctgaat gcattgcgtc agattgggcc attgctattt caacatgttc
     2521 attttttctc tgttctcttt gaaaatcact catgattaaa cctacctttt cgtcatttct
     2581 aatttaaaac cattttttac gtctgaaaaa gatgattaat acgacagcta taacaaacat
     2641 tacagctaag caaatataat atccataatg taaatgtaat gccggcatat ttacaaagtt
     2701 cataccataa atcccagcta tgaatgttaa cggtgaaaat ataactgata ctaacgttaa
     2761 tacttgcata atactattca ttctaaatga cgtgtatgac tcaaaatttt ctcgtatttc
     2821 gtttgtcatt tcttgagcag tacgaatgat attacgttgc ttaatcaagt ggtcatcgat
     2881 atgttgaatg tatagcgaat gtttattatc tataatcaaa tcaccatttt gtttcattgt
     2941 atcaattagc tcttgcatag gaaacagtac acgttttact ttaatcaaat ccgaacgtaa
     3001 cttaaagaca ctatccatga ccattttatt aaagcgatca tctacatggc ggtcttcaaa
     3061 atgataaaca ctatcttcaa gtgcatatac aaagttgaaa tatttatcaa ccatcatatc
     3121 taaaattaat atgacgacat ctgcacaatc taattctgca tctaatgtat tcatatactt
     3181 atagactact ttatttaatg attccaacgt ttgatgatga tatgttacta atacattgtc
     3241 ttgtataaaa atatttagtg ctattggtga atagtttgac cccataatac tatgaaatac
     3301 taagtattga taatctttat aagatttata tttagctcgt ggcataccgt taattgcatc
     3361 atccacttct aaatcattaa aattaaaatg tgctttaaac cattcatttt cttgttcatt
     3421 cggttcatca aaatcatacc aaacaatagt cgcatctttt ggtatctctt tgatatcatc
     3481 aactacttta aacggttcat atgtagtttg ataccgtatc tttaaagcca tcgatactcc
     3541 ccctaaataa cgaattctct attattttat catgaattaa ataacgtgta tgtcttaatt
     3601 tattttagta tgatagtcac taaggagatg gttattatca aacaactttt tacacatact
     3661 caaaccgtaa catctgaatt cattgaccat aacaatcata tgcatgatgc aaattataat
     3721 atcattttta gtgacgtcgt gaatcgtttt aattacagcc acggtctttc tttaaaagaa
     3781 cgcgaaaatt tagcatatac actatttaca ctagaagaac atacgacata cctctcagaa
     3841 ttgtctcttg gcgatgtatt tactgttact ttatatattt atgattacga ttataagcgg
     3901 ttgcatttat ttttaacatt aactaaagaa gatggtacac tagcatcaac aaatgaagta
     3961 atgatgatgg gaattaatca gcacacacgt cgttctgatg cttttcctga atcattctca
     4021 acacaaatag cacactatta taaaaatcaa ccaactatca cttggcctga acaattagga
     4081 cataaaatag caattccaca caaaggagca ttaaaatgac agatgcatta caacaaaaga
     4141 ttcatattga attactagat ttattagatg atgttaagtt tgaattaaca gaattaaatg
     4201 cacaaaaagg gttatacatt aacggaccag caaatcagct acttaagcgt ggcgttcata
     4261 tggcttatgt tcaaggacaa aagcaagcca tcgataatat tatgactatt gtggaacagc
     4321 agcttgaaga tcaacatttc ctagaagatt atgataaatt tcaaaatgag gttactcatc
     4381 gcaattatga taaaacagct aactttgcag aacttagtga tattccacgt caattcgata
     4441 attttttaga tcaattttat caaattaaag ggcaatactt tatcatcaca catatcaata
     4501 cacttattgg tgattttcac tcagaagctc attaacaatt agtctatata acccttgcta
     4561 tattttcaaa aacaaaaccc aattacgttt tcatgtcaaa tatcatcttg catgaaatcg
     4621 taactgggtc aattgtatgt tattagttat tttgtgttac atcctcatct atcgatttgg
     4681 caatttgttt aatagcttta tgtgattgtc taattggata aattggaaaa tcatgtacca
     4741 tcttaggata atcataaaac tcaatgtatt gatgatgttg caacatcatt tgttcgaata
     4801 gcttcatatc aggatgtgtc atttcacgtc caccaccaaa catataaact ggtggcaatc
     4861 cttctattgt gccattaatt ggcgatatgc gcttatctgt taatggtagg ccattcgccc
     4921 attttttcat aatctcattg acaccaaact gacttagaac cgcatcttgt tcgattaagg
     4981 cgtccgaaat atctttatta gatagtgttg catctaaaat tggtgagatt aaatacaatt
     5041 tattcggtaa tggctgttga ttatctaaaa gagattgtac aaaggataat gccagtgcac
     5101 cacctgaacc atcacccatg actacgacat tttgatgtcc tacttcagat actaattgat
     5161 cataaacacg ttgtatcgct tggaaagtat cgtcaatatg aaactctggt gtctttggat
     5221 agataggcag tacaacctca tataatgtac ttaaagtgat tttatccaac aatctccaat
     5281 ggaacggtga tggttgtagt gcattgaatc caccgtgaat atataaaatt ttcttatcaa
     5341 tttgatgtct gaaattaaag cgaaagactt gcatatcatc taatgataat ttttctaaat
     5401 ttgctttaac atttaatgtt gaaggctgct tatgtttttt tctattttca atttctcttt
     5461 tataaaaaaa tctttcaaca tcttgatcat ttttaaacat aatcgagcga ttgtgaagca
     5521 aatatttatt gacaacgcta ttcataacac ggtttctaat caatgtctta acctaccttt
     5581 atatatttta tgtatccaat gattgtctat cccctacatt ctttgccaaa aaaagtatat
     5641 aatgtagaag atattttctt tttcactttc aaatttaaga ctacaattga acagtgattt
     5701 ttcatcatta taacagacaa ctagacatat tgataagtaa agaaaagaac tttatacgga
     5761 ggtgccttgc atgacaaatc caaatcaacg attagaacca tttgatgaga catttcaaca
     5821 accgaatatt catcgtggta agcgatatgg taagaaaaaa cgttcattgg taagcatgat
     5881 tattcaaatc attgttgtta tattaaccgc aatcgctgga tacagcatgt ggaaacaacc
     5941 catttttaat attgtcttca ccaaagaatc tgtagatttt gatggcatta aaaattttaa
     6001 cgagacaatt ggaaaaatgc atcaattaaa tattaatttg ggtaatatca atgatttaca
     6061 acaaggaatc gatcgcttaa ttttagtctt taacggtttc tttattctat gtatcgttag
     6121 cctagtcata acaatactga caattgtatt taatcgcaca gttctaaaag tagtaaatat
     6181 tttagtgtta acaattatgt tagtcattac tttgtatttc agttatatta ttaaaacact
     6241 cgcacaagaa attgcagatg ctttaaaaaa atattattta aatgtaccac cagatcaagt
     6301 gattacagaa gctgacgcta ttcataacgg catcatttta ctgggctgta gcatcggttt
     6361 acttgttata agcttattct tccgtaatcg catgcctcgt ttaaaataat gttatatagt
     6421 agggattggg acagaaatgt tattttcaca aaattcattt cgttgaccca cttcctgctt
     6481 tttctttatt aatgacctac tacgtcaatg ccaaatttta atataattcc tcttttattt
     6541 tataaatcaa caaataaaaa aagtatcgat aaccatattt ggcaccgata ctttaaaaat
     6601 catgtcccca caacataatt tttgattagt cacacttcaa ttaatttatt ttatttattc
     6661 atgattgtta tttgagcttg cttctaactt ttgagctgta gcttttgctt ttttattact
     6721 ttgtaaaaat aaacataaaa ttaaagcgat gatgctaaat atcgttgcaa ctataaatgc
     6781 atcattaata ccttcaactg ttgctagttt atttacaaat tgtaataaca ctttcattgc
     6841 gccttcttga ccaccatatt gcgatgctaa ttcacgcata tgatcttgta caacaggatt
     6901 cgttttatct aactcttccc caaaagctga taaatgttgt gttgtttgtg ttgtcataac
     6961 agtaactaag attgctgtac ctatagagcc tgctaattga cgcatcgtat ttaagaaagc
     7021 attaccatga gaggcaagtc gtcccggtaa cgcattaata gctgcagtta ccattggcat
     7081 cattataaat gccataccaa atgaacgaag tacatagata cccatgattg tcatatatgg
     7141 tgtatccata tttaatttag ttaattccca tgttgcataa gtcatgacag caataccaaa
     7201 gatagctaat ggttttaaac caatagtatc taacaattta cctgcaaatg gtccaagtag
     7261 acccataatt agagaaccag gtaataataa caatccggaa tctaatgctg agaatccgcg
     7321 taaattttgt aaataaatcg gtaataaaat cataccacca tataaactta acattacaac
     7381 catattaata attgttgtta atgtaaatgt tgggaatttt aatacttcta aattcaacat
     7441 tggtgatttc attcttaatt ctcttattac aaatagaata ataaagataa taccaatcgc
     7501 aaacattgtt tctatctcaa cagaacccca tcctttgttc cccgcttctg aaaagccgta
     7561 taacaaagca ccaaaaccaa tcgtactaaa aatgatacct gggatatcag ctttagggtt
     7621 tgttgtatat tgatataact taaaccatac aaaaccaact aagattgcaa taataccgat
     7681 aatgaacata ccgtaaaaca tcacattcca atggtaattt tgtacaatat aacctgataa
     7741 tgttggacca attgcaggtg ctaaaatcat tgcgataccc attgtaccca tggcagcacc
     7801 acgtttttca ggtggataaa ttgtaataat aacaattgaa cctaatggca ttagtacacc
     7861 tgcaccaatg gcttgtaata cacgtccaac catcatgatt gggaaattca ttgaaatcgc
     7921 acagattaat gaaccaattg taaagagtac taacgcaact aaaaataatt ttcgatatga
     7981 atatttatta aatagatacg ccgtaattgg tattaaaata ccgtttacta acatgaatcc
     8041 cgtcatcaac cattgccctg ttgacgcaga aatattaaat tccgtattaa tttttggcaa
     8101 agcaacattt aataatgttt ggtttaaaat cgcaataaac ataccgaata ataatgccgc
     8161 taatatttta ccgcgtgaaa caccttcacc aaaaataaag tttttatgtt ctttttttat
     8221 tttttcattc actttatatt cttctgattc aggattttta gcagcaactg cttcctcacc
     8281 attattatta gtgaatgctt cttgatcttt ctcagacccc tttgtgtaac catttagact
     8341 aacttggcta tgatcatctt gattgtcaac acgcaactct tcatgcgtca tacgttgtgt
     8401 cgattgatca gttgttttgt ctaaatcact agctttaaat ttagattgat ttgattgacg
     8461 tgtcgtaaat tgttgttcct tttgttggcg tttgcctttt tttcttgatc ttattaaaaa
     8521 taaattgata accccaacaa taatgagcgc taaaataatg tagctaataa tgaaggtcgt
     8581 agtcatttaa tgaccccctt aatttttatg gatttttact tcagcgttca ttccaggaac
     8641 aacttgttta gacggttctg attctagagt gattttaaca ggtattactt gagaaacttt
     8701 agtgtagtta ccatcactat ttgatgatgg cattaatgaa aagcttgcag cagttgcttt
     8761 tccaatacta tcaactttac ctttaataga agctttttga ccgtcaatag tcacatcaac
     8821 atctttacct acttcaacat ctttaatatc tttttcgtca atatttgctg ttacatataa
     8881 atcatctaaa ttgtatgcat aagcgattgg gttaccagct tgcaccattg aaccttccat
     8941 accatctaat ttggcaattg tacctttttg aggcatttta agatccatat ctttcgtttc
     9001 gccatcttga ccttgtacag taacagttgc tactttgtca cctttatcga gtttgtcacc
     9061 ttgtttaaca ttaagtgatt taatttgtcc agatgcagga cttgctattt taatttgatc
     9121 gccatttact tttgcattat cagttgttac atagcttgtt gttttattcc agaaataaaa
     9181 gccagcaatc ccaattgcta acagtacaac aaccgtaatg acattaatta atatcatctt
     9241 tttcatgaaa catttcctcc tatttgcttt taaaattcac tttatttatt atagagacat
     9301 gataactaaa aaaacaccaa caatttttat aagagtgttg gttaaaattc acaaaggtgt
     9361 aacaatatga aacgacaagc caaaatagaa attcaaaacg cacttgttga tttgatggct
     9421 gaatatcctt ttcaagaaat atccactaaa atgatttgcg cgtattgtaa tattaatcgt
     9481 tctacttttt atgattatta caaagataaa tttgatttat tggataccat caactcaaag
     9541 cataaagaaa aattccaatt tcttctaagc gcgctacatc ataatttcga aaatattaaa
     9601 caggataagc ttaaacttta taaattcttc attattattg ctaagtatat caagcacaat
     9661 gagcagtttt tcaaagacat tttagttacc tatccaatga aaacattatt catagactat
     9721 ataaatttag cgagagatta ctatcaacaa attatgaatg actatagtac tacagtaacc
     9781 aataaacagc tttatgttac atatatcata ggcggtcaag ctggggtgtt tattaattgg
     9841 ctttcgaatg gttgtaatga atcacctgaa gaagttgctg acattttatt ggctaatacg
     9901 attaaacttc aaatccaata atacggtttc attctaataa agaaatgcta taaaacaact
     9961 agataaagtt aaaccaaatg gatagataat agtacatatg ttacggtgct atcaatataa
    10021 aaatgccgag atttccacat aataatctcg gcatttttat ttttgatgct aaattcatag
    10081 gtctatgaat agctcaagtt ttgtttttta atttttcaat tcaatataat agaagaaact
    10141 atgtttatca tcgatttgtt ttaaatacct tcatattata aatctgtaaa ataattaata
    10201 taactgctgt agctataata ataattatgt aaggtatagg attatcttca ccttttaccc
    10261 ccactaatgg cgatgcaaca ccaccaaata aaaactgcac taaacctaat aaacttgatg
    10321 aactccctct accactacta ctttcatcca tagctattgt aaaaccaagt gtcgcaacac
    10381 ctgtcactgg tgctattaaa atgataaatc ctatcgctaa aatccaaaag ttccaatgat
    10441 taagcaatac aatcgtcact aaaattacac cgataatctg aatcatcgtc atgcctctca
    10501 tcaatttttg tgagtctata aaatcaacaa ggtacccagt aagttgactt gatataatga
    10561 gcgtaatacc aattcctgca aacatccaac taaattgtat tgcagtcatg ccataaattt
    10621 tttgaataat aaacggagac gcagaaatat aagtaaataa tattacaaat gtcatacctt
    10681 gaatgagcat tggtaataca aaacgtggcg tcttcaataa tattttgaag tttttaaaca
    10741 ttgtttttaa tccactactt gactcacgat ttgtcactgt taatgattca ggtactttta
    10801 ataaagaacc tatgaccatg acgaaaccaa agatagtcag aatgacaaag accatacgcc
    10861 agacagaata atttaaaatt atgcccccta ttgttggcgc aacaactggt gcaataccat
    10921 taacaagcat caataatgcc ataaacttag ttagttcatt accactatac atatcacttg
    10981 ctatagctct tgaaataact gctgctgcgc cacctgtcac tccttgaaga aatcttaatg
    11041 caaccatcag ccaaatatta tgtacaaaaa caatacctaa acttgctaat gtaaaaataa
    11101 tcatagctat aataagcggc tttctgcgcc cagttgaatc tgaaattgga ccagcaaaca
    11161 aattaccaaa cgctaaacca atcataaaca tcgataatgt taattgtgcg ttagacgtcg
    11221 ttgtttgaaa atcatgtcta atatctgata atccaggcaa aaacatatct atcgataatg
    11281 ctccaatggc cgtcaaagat cctaaaataa taataaatat cggtgaacgc ttgttaaaat
    11341 gcgttaaatc attcatatga ctatctcctt tatataatga ttagtttata actatcacga
    11401 acagaaacta aatttaaata tgctctttta tatattagca ttactcacaa gtttcatgaa
    11461 catcattaca acttataaaa ggtataaaat gatataaacc tcagcactcc ataaattttt
    11521 tcacaaaaaa caatcccttc aaagtaattc acactaattg taaatacttt gaaaggatgt
    11581 tgtttgtatt ccatgacaca tgcatcttat ttaagtttta tactctattt ttctgatgtc
    11641 ttgattaatt gcaagcggtc ttgcgagcct tgttcaagct catattcttt atctacttgc
    11701 ttaccgtttt catttattaa tcttacggtt gcggtaatct tatcaccatg tttttcagca
    11761 tctataattt gtggtgaact aatcatcatt aaacttcctt tagatacacg cttctttaca
    11821 tcatcataaa aagatgatcc tttttttata tatgacgata caaaatcaaa atctgactga
    11881 ttaaacgcag cattattcgc taaagaatat ccagcaaaga attctatcaa tttgttcttc
    11941 aagctgtttt cttctttttc tttcttttca acatagtctt cgatatcttc actgtcaaaa
    12001 ttcaaagtta tctctgtatt gtattttaaa tcgctggctt taatcgtctt cgtctgtgtt
    12061 gtaaatgttt tacctttcgc tttacctgaa gctgaaatgg taatatcttt attttgtgga
    12121 tatggaccat acgttttgga acttgaaaat gccatttcac ggtcatttat agttactttt
    12181 ttagaactat catttaattt tgtatcgcct tttaaagtta cagttatatt tgcttcttca
    12241 aaatcttcag taacatctac cgtttcagaa ttactttgtc taaaatcaaa ttttaaatag
    12301 cctgcaaaat caccgttctc agttgatttc atagctggaa ttctatatgt ccccggtata
    12361 aaattaccta ttggcgtcac tttatttgct tcagctataa ccatcttttt cttaccacca
    12421 gatttaaact catattttgt tttttctttc ggcttaacaa ttggttgctt ggtaggtgca
    12481 gtaaagctca tattatcgaa aaagatataa cgcgtgccat ttttacttac acgtaatata
    12541 ttttgaccag atctggtttg aatataagaa gctacgctgg tcttactctt attcaattta
    12601 tgtaccgtat ttttaaggtc gctgacaaat tgcttaagcc caacttcatc tttgatatag
    12661 ttaatgtata ctttcgcttc ttcagaatct actttattat ctttagtact taataatgtt
    12721 gctacttttt gtttatcatt attctcaatc gcattcacta atattttagt ttgcgcttca
    12781 ggtgaattga agtttcttag taaaaagaac aatataataa ttaagattaa aatgaaaaaa
    12841 ccaattgccc aaggcaccat ttttctcatc ttgatgttat tagtttgtat atcttcatcg
    12901 gttgactttc tatataatgc ttgacgacta tcaaatttat gtccacattg tgtacatatc
    12961 tgtacatcat cttgtgcttg ttgtccgcac ttcgggcaag atttcatact ttcacctcaa
    13021 ttctgttcct cttcaattgt tatatgtata tcacaaaaat tatttcatta ttttaccatt
    13081 attattttac aaatattatc ccaagaatac aattcaagaa atgattaact ttactttaga
    13141 attgtacctt attacattat ttatgaaagt actctctcaa ttataactaa aaagaaggtg
    13201 ttcaatacat acatatttag tcttatattt gaacaccttc aaaaacctat ttatgattgc
    13261 tatatgtttt aatgcgatga ttgatgactt ctaatacttg ccttacattt tcaatatctt
    13321 cagaatttaa atcattagta atatcttgcg caatatctgt cataatcgca ttacgttctt
    13381 tcaaatatgc tctacctttg tcagttaagg ttatgaattt caaacgttga tcaatattta
    13441 aatttggttt atctaactta actaatttag catcgattaa ctttttaatt cgtcggctta
    13501 ctgcggcctt atttactcct tgtctttgcg tgatttcact aattgtcaaa ggttctttat
    13561 ttaacatacc taatacgtta gactgctcta atgaaatttc atattcattt tgtaaatctt
    13621 tcaacatttt tgcagttaat gcgttaacgt tatttataaa ctgctctaaa aattgaatat
    13681 ggtcttgtaa atgttttacc ataattattt ctccctaatt actattttta taaagtatcc
    13741 tcagcaatca ttgtatcgca catctaaaaa acacaaaaat attgacccat atataatata
    13801 acaaattaag acttacatgt tgctgcgttc tccttgcttt aataaattaa atcatctaca
    13861 tttatattta atatttatta taagcgaggt gtcagtatgt tattgaattt attattaata
    13921 tttatactta tcgttatttg tccgttaatt ttcaactata ctctgacgtt aaatttaaca
    13981 tgctacatct tagttgtcat atcgatattt ttgtcactca tctatagcat catgacctat
    14041 actttccctt ggtctatgtt tgtaacattt ataataatca gtattatcat gctattcaaa
    14101 catagatatt tattttctca tacttctagt cgcttattta ttaaaaaatt attatctttt
    14161 tcatataaat ttgtactcta cacatcagca cttctattta taagtacgat tcagtcacct
    14221 attgtgaaag gtttgtctat gtggttagct atattatgtc tttccctgtt actaaccttt
    14281 atttgttatc tcgtttggac agtatcatat agacatagat catacaacaa agaagttgat
    14341 atcataattg tgttaggtgc tggtattttt accgaatttg tcacaccaat gcttgcagcg
    14401 cgtttagacc gtgcacttga tatctatcaa caacaagcat cagcaacaaa gattattgta
    14461 tctggcggac aaggaccaga cgaaccaatt tctgaagctt tagctatgca acgttatttg
    14521 attgcacatg gtgttgataa aacagatata ctaatggaat ctcactctat aaatacatac
    14581 acaaactttt tatattctaa acaaatcatc aacaatttgt atcatgaagc agttaaaatt
    14641 gtttgtgtaa caagtcagtt tcatgtattg cgcgccttac gactagccca aaaattaggt
    14701 ttatctatcg atggtgtcgg aagtcataca ccatatcact ttttcttaca tgtgctcatt
    14761 aaagattatc taggcgtaat gtatcagtat agattgttat taacgttata ttgttcaagt
    14821 agttggttta tatgtcttta cgccgctttt atttataata gttaatccac aaacaaacta
    14881 ttattatagg ttgtttctaa tattgcgtca aactatcttt aaatattcgt taatccttga
    14941 ttagacaatt gttatattga aaataaataa actaaagcac gtcatcattt tatataatag
    15001 tgacgcgctt ttttgtaaac ccaaatcgta ttctgttatc taacttttat actactatgt
    15061 cttaaatcat taacatttag cgtctttaca agccactaat cagttatttt gccatcttct
    15121 aattcaatca ctcgatctgc atattcaaat aatcttcgat cgtgtgtaat cataatacca
    15181 atcatttgtt gttctttaat ttgttgacgt atcatctcaa caacttttgt tgctctatcg
    15241 gcatctaaac ttgctgtggg ctcatctgct aaaatgattt tcggattatt cataaatgct
    15301 ctcataatcg caacacgttg cttttcaccg ccagataact gatgcggata tacattcaag
    15361 cgatcttcta acccaatatt tttcaaaagt tgtattgctc ttgttgaact ttgttgtttg
    15421 gtcattcccg cttcttggcc tacgagtgtc aattgctcta tcacttttaa ataaggaact
    15481 aaatgtgaag attgaaaaat aaaaccaata tcttccaatc gcaaatcact aggacgatgc
    15541 tgtttatcaa acaatggtgc atcattgtaa agcactgtac cactcgtttg acttaacaat
    15601 ccgcctaata tcgttagcaa tgttgttttc ccagaaccag aggcaccatt taaaatgaca
    15661 aattcccctt gttccacttc aaaattaata ccttttaaaa cttttgtttc agacaaacct
    15721 tctccgaaat ttttgacgat atctttaacg actaatgcca ttattctgca cctccaattg
    15781 cttcgatagg atccacttta aataatttga taaatgatag tgaggcacct aaaatcgcta
    15841 ctaatataaa tatccccacc attaataaaa tattttgcgt tgttaagtaa aaaggcatcg
    15901 ttaccggcat cataaatgat agtcctacta tgatgatcac agcaatacct accccaatta
    15961 ttgttagtgt taaaatttgt aaaactaacg cactcaataa atgtcttgtc ttaataccaa
    16021 ttgctttcaa aatgccaatt tgtgatattt tttgaatcgt cataacatag aaaaatgcac
    16081 ttagaacgat tgctgtaata gcaaacaaac taacaatcat catatttaac ggtgcttgct
    16141 ctgcttgata actcgcaata ttacctgtta aatctttttc actcacaact tgaacgtcat
    16201 ttattttatt aagctcatca cgttgttgtt gtgttaaatt tttcactgga taaaatgccg
    16261 tattaacctt attcaatgca ttaaatccgt tatcgttcaa tagcacaatg gaactatgcg
    16321 catacattgt gtcgtttaaa ataccagaaa ctctataact agttgaatta tttttaaaat
    16381 gtaatctatc cccgactcta atgttgtctg cagctaactt atcattaata gcgacctcat
    16441 gagcattttt aggataatca ccttttttaa gttgtatgcg ttgttgttta acgtcattca
    16501 ttgtaatcac atcttgttct ttattagata atttcaatgt ttgaatattc attttatatg
    16561 gttcttgatg aattaccttt ttaatttgat tctgttgcgt atcactaagt tgcgatttct
    16621 caatttgcgg ctctttcatt ttttgaacaa catattcatc attatcaaag tgttcaaata
    16681 acgaaatatt ctccctacca agcccttgag ctaaaccact aataaataac accataatac
    16741 ttaataaaag aatgattaat gtgattaaaa tataacgaaa tttgtaaaac ataatctctt
    16801 ttatcgctaa tttcatatcg attcacttct ccctatttct tctttagcgt cagtataagg
    16861 ttgaaatatg aactcaatat gaacaattgt ttttttacgc aaaaatattt tacatgtata
    16921 ataaatgaaa aagataaggg agtttatagc tatggtgcaa tgtcttgttg tcgatgacga
    16981 tcctcgaatc ctaaattata tagctagcca tttacaaaca gagcacattg atgcatacac
    17041 acaaccaagt ggtgaagctg cattaaaact tttagaaaaa cagcgtgtcg atattgcagt
    17101 ggtagatatt atgatggatg gtatggacgg ctttcaatta tgtaatacat taaaaaatga
    17161 ttatgatata ccagttatta tgttaacagc gcgggatgca cttagtgaca aagagcgtgc
    17221 gtttataagt ggtactgacg attatgtaac caaacccttt gaggttaagg aacttatttt
    17281 tagaattcgt gcggtattac gtcgatataa tatcaattca aattcagaaa tgactattgg
    17341 caacttaacg ctaaaccaat cctatttgga actccaagta tctaataaaa cgatgacgtt
    17401 accgaacaag gaatttcaat tattatttat gcttgcagca cgtcctaagc aaatctttac
    17461 tcgtgaacaa ataatagaaa aaatttgggg ctatgattat gaaggagatg aacgaacagt
    17521 tgacgttcat attaagcgac tacgccaaag attaaaaaaa ttaaatgcca cacttacaat
    17581 tgaaacagta agaggacaag gctataaggt ggagaatcat gtttaaaaca ctctatgcta
    17641 gaattgcgat ttattccatt acggttattt tatttagtgc attaataagt tttgtattaa
    17701 caaatgttta ctatcattat aatttaaaag catctaatga cgcgaaaatc atgaagacac
    17761 ttaaagaagc aagacaatat gaacaattag ctaaaccaac gcacattcaa caatatttca
    17821 aacatttagg gcaaatgaat taccaaataa tgaccgttga tcaaaaaggt cataagacat
    17881 tttatgggga gccatttaga gaagatactt tatcacaaaa tgctattaac aacgtattaa
    17941 ataataaaga ttaccatggt attaaagata aaccattcgc attattcgta actggatttt
    18001 tcgacaatgt aacagataat actgttggta ttaactttaa gacaaaagac ggttcaatag
    18061 cagtattcat gcgtccagat attggagaaa catttagcga gtttagaaca ttcttagccg
    18121 tcttgttaat gttgttatta tttatctcga tttcattagt tatcgcatca acctattcaa
    18181 tcattcgtcc agtaaaaaag ttaaagctag cgaccgaacg cttaattgat ggtgattttg
    18241 aaacacctat caaacaaaca cgcaaagatg aaattggaac attacagtat cactttaata
    18301 agatgagaga gtcattggga caagttgatc aaatgaggca gcattttgta caaaatgtat
    18361 ctcatgaaat caaaacacca ttaacacata ttcatcattt attaagcgag ttacaacaaa
    18421 cttctgataa aacattaaga caacaatata taaatgatat ttataccatt acaacgcagc
    18481 tgagcggatt aacaactgaa ttgttactct tatccgaatt agataatcac caacatttat
    18541 tattcgatga taagattcaa gtcgatcaac tcattaaaga tatcattcga catgaacaat
    18601 ttgctgctga tgaaaagtcg ttaatcattt tagctgacct agaatcaatc aatttcctag
    18661 gaaatcaacg tttgttacat caagcattga gtaatttatt aataaatgct attaagtata
    18721 cagatgttgg tggtgccatt gacatcgctt tgcaacatag tcacaacaat atcattttca
    18781 caataagtaa tgatggttca ccaattagtc cacaagctga agcacgttta tttgaacgtt
    18841 tttataaagt gagcaaacat gataatagca atggtttagg tcttgcaatt acaaaatcca
    18901 tcattgaatt gcatcatggt acaatccaat tcacacaaag taacgagtat gtaaccacct
    18961 ttactattac actgccaaat aattcacatt aatctctacc tcctgaaaaa acatttctgt
    19021 ttttatttca ggaggtatct tttatattga ttagcgcttt ctagccactt aacatttaat
    19081 ttctaccatt caacaaaatt taaatatatc gatggtcatt tatggtttaa taattttgaa
    19141 ggaggcatta acctatgatg aaactcaatt tatttatcaa tgcaaatgag acggaatctt
    19201 atattgatat acatgcacct aaaatgaatg atcacgttca aagtattatt aatgcagtca
    19261 atgatttaga caaatcacat acattagtcg gatacataga caaagaaatc catatcatta
    19321 atgtatcaga tgttataaca ttccaagtta ttaataaaaa tgtaacagcc attacaagca
    19381 atcaaaaatt caaactaaaa ttacgacttt atgaactaga aaaacagtta ccacaacatt
    19441 tcattcgcat ttccaaatca gaaatcgtca acaagtatta cattgaaaaa ttattattag
    19501 agccaaatgg acttatacgt atgtatctta aagatgcaca ctacacctat tcttctagac
    19561 gttatttaaa atctataaag gagcgcttat caatatgaaa aatttgaaaa actcattatt
    19621 tattagctta atcattggtt tgtccctttc actgttcttt agtatgttat ttgcagacgg
    19681 caaatattat ccactgaacc cacaatcaac tattggcatt ttgtattata atcatttcac
    19741 agaaactacc gtaatgttga tttctatcat tttatggtta ctcataggcg tcgtcttttt
    19801 ccttggagat tttatcttta aatacacaga ttggagcatt actaaagcaa ctataatgca
    19861 tttcataaca acgtatgtcg gattcttgcc tttagcaatg cttgccggtt ggtttccact
    19921 aacagtgcat tatctaatca tattcacaat tatcttcatt gtgatttatg tattaatttg
    19981 gataattcaa ttctttaaaa acaagaacta cgtagacact attaacgagc aactgaaaca
    20041 attaaaatga tttttattaa ccactaaaaa acggtaatgt ctatcacatt aaaaaagaca
    20101 ttaccgtttc gtttattatt taacttgtaa atacttagtt acttcttcat taattttgtg
    20161 atataaatcc tcatgatctg ttaagcgata accaaatgac ggcaccattt ctttaatctt
    20221 tggttcccac cctttgaatt catcacgata gcatcgctgt aaaacatcaa acataatatc
    20281 tacagctgtt gacgcaccag gtgatgcacc aagtaatgca gctaatgtgc catcatctga
    20341 cgtaatgact tcggtaccaa attgtaagtt acctttagaa tcctcagtat ccttgattac
    20401 ttggacacgt tgccctgctg taatcacttc ccaatcttca tttttagcat ttgggaaaaa
    20461 gactcttaaa tcatccatac gctcatcatt agataacatc aattgtgaca ctaaatactt
    20521 cgtaagactc atttctttga tacctgcaga taacatcgtc acgatattat ttggtttaac
    20581 cgatttaatt aaatccatat gtgaacctgt ttttaaaaat ttaggtgaga aacctgcaaa
    20641 tggaccaaat aacaatgaac gcttgccgtc tacaaaacgt gtatctaagt gcggcactga
    20701 cattggtggc gcacccactg ctgcttttcc gtacacttta gcatgatgac gatcaatcac
    20761 ttcttgattt gtacagcgca ggaataatcc acttactggg aaaccaccaa tatgttttga
    20821 ttgtttaatc ccagtctttt ggagtaatgg taaactcgca ccgccagcac caataaatac
    20881 aaaatcagat tcatatgttg tcgtttcatt agtttctaaa tctttaactt taactagcca
    20941 tgccccagat ttctgtttct tgatgtctaa tacattctgc ttatattgaa ttcccacacc
    21001 acgttgctct aaattttcaa ataactttgc agttaacgca ccaaagttaa catctgtacc
    21061 agtttcatca taagttaaag caattggttc atcagactta cgaccttcaa tcattaacgg
    21121 tacccaagat ttcatttctt cttcatcttg cgataatttc attttttcga ataaaacact
    21181 tttctttaac gttgcaacac gactttttat aaaagctaca ttatcttcgc caatgacaaa
    21241 actcatgtgt ggcaccgctt gaataaagcg atctgggtta tctaattgtc ctgttttaac
    21301 taaatatgcc caaaactgtt ttgaaatttg gtactgctca tttatcttaa ttgctttact
    21361 acaatcaact gtgccatcct taccttcttt tgtatagttc aactcgcaaa gtgctgaatg
    21421 ccctgtaccg gcattattcc atacatttga actctcttcg ccaggttgtg ctaatttttc
    21481 aaacacttta atatttttct caggtgataa ttctttaagt aatgttccta atgttgcact
    21541 cataataccg ccaccaatta agatgacatc tgttttgcta tgttgtgttg tcataacaaa
    21601 tacagtcccc ctttttaaaa tgaataatgg aacgttcgag aaatacgaca tattaatacc
    21661 actttaaatc aataagtgtc tcaataggtt cccatggtga tgctctatta ctatctacta
    21721 tgtatattaa tattttataa taacgaaact actttaatta tatactttat gtgcgatagc
    21781 acaaagacaa aaccacaaac cttaacagct atcaaccaat ttaatttgat tgctgattac
    21841 tcactattca ttttaacata aagcgctacc attattataa tgtcaaactt ctttcattaa
    21901 tgatccataa aaaaaagatg agatgttaga aaacatctca ccttctgtat atttatattt
    21961 tgaaatacgt ttttttataa taatgacaag atgaaagtcc aaatacagat gaatattagt
    22021 aaacatacac tgtatttcaa tgtcattttt aataattctg attccttacc aacttgtttt
    22081 actgctgcag ttgcaattgc aattgattgt ggtgaaatca attttgccgc tacaccacca
    22141 actgtattag cagatacaag aagtgaacca cttgttccaa tttgttgtgc aacagaagct
    22201 tgaattggtg caaataatga attgttattt acaactgatc ctgtcataaa cacacctatc
    22261 caacctaaga ttggtgatag aactgggaag acattacctg ctttagctat accttgaccc
    22321 attgctgcac ttaaaccacc ataagttgtg attttagaaa ttgctaagat gaaacaaatt
    22381 gtaagaactg gtaaccacaa ctctttaaat gtaacgccga ataatctaac agcatcttta
    22441 aagttaacct ttttagacat taaaattgta ataataatag ttaataaaat agctgtacct
    22501 gtttgtccaa taatatttaa agtcaatact aatggtttat gcgtaacttc gctgattgtt
    22561 ccaggtaagt tgaatttaaa tactaatgat gataaagcac catttggtaa gaataagttt
    22621 ttaaagaatg gcgcactcca aatcattaca atgactgtta atacaatgaa tgggctccat
    22681 gcatgtaata ctgcttttgc agaatgtgct tttgcaggtt caatttcttc atctttatta
    22741 acacgataaa tgtgttttgg ttggaatttt ttagaaaata ctgctaatgc taacattgtt
    22801 aataacggtg gaataatatc tgctaattca ggtccactga atacagttaa taatccttga
    22861 gtaagtgtat aagtgattga aactactaaa attgctggta atgtttcttt aacacctcta
    22921 aaaccatcaa taataaagat aagtaagaat ggaataatga aatttatgat tgccaatgtt
    22981 aaagttgctg attgagaaac acctaatact gaaacatctc caggtaattt caacgtttct
    23041 acaacaccta cagggatacc aatcgcacca aaagcaccag acgctgcatt tgcgactaaa
    23101 cataacatcg cagcttttaa tggattaaat cctaattgtg ttaataaaag tgcacaaatt
    23161 gcaattggta caccaaatcc tgctgcacct tctaaaaatg cgttgaatgc aaatccaata
    23221 agtaaaactt gaatacgttg gtcttgtgaa atatttgtaa tactatcttg aattgttaaa
    23281 aattgtccag attcaacagt aattttgtat aacaatactg ccatcataac gatatagcca
    23341 atcggaatga taccttggaa gaaaccttcg actactgcac cagaagcgat accaactggc
    23401 aatttgaaaa atggtattgc aattagtaat gttacaacaa gtgttgtaat agctgcataa
    23461 atacctttca ttttaaaaac agttaaacat agtaaaaaca gtacaatcgg tattgctgca
    23521 attaaagacg ataataataa attatcaaat gggttaaacg tatttactaa cataggctga
    23581 ctcttctttc cataaagtag tagtgtaatt gcttcattaa aatcatgctc tggctatagc
    23641 attttgttat cgataacatt ttacagaaca gtgagattaa tgtgattttt ttcacaatta
    23701 aaatatacca ttttttcaga ctattaacaa tatcactttc ctatttaatt gttttatttt
    23761 tcattttaaa tctgttcatc aaaatgaatt gtacttaatg taatgaagta acttatacta
    23821 ttcatgaatt ttacataaga ccgtgctaaa ataaggaaga tactacgatg agaaatggaa
    23881 gttatagcaa tgattaaaca gataaatgtt tctaatatgc aaaagtttga atctcaatta
    23941 atgaaagctc agtctgaagg ttatacacat gtcgttccat atgcaaatga gataatgatt
    24001 tatcaatcga tgttagatgc ggttcaatta tatcctaagt ctatcgtggt tgattatact
    24061 gtggatggac aatacaaaaa tgattgtcac tatttcggtc aatcatcgat caacattgcc
    24121 gattgggctc aaaacaataa ttattatcct aacctgatat atgcgattca acagactctt
    24181 gatttaatac attattactc tgtagaaacg attttcgatt tagctttatt gacacttctg
    24241 aaaggtgatt tatcaatcga tggtcatgtc gtttttgatt ttaaagcccc tatagcaaca
    24301 agtgcttcaa tatgggaaac tattaaaact attgaagatt ttgatatgat gtctcagttt
    24361 tatttgaata aaatggctta tattgatcat catcctatac cttttcgcaa cctttttatc
    24421 gaggattcag aacaacttaa ttcgccagat aattggttgt attctaccaa gtttatgcta
    24481 cctaaatggt tatataaaat agcaaagcaa cgtgctgata acaaacaatt acaaaacttc
    24541 ggcctgtata cgaagcaacc aaacgtttta aaagatcaca tcgtttttat cggtgatcat
    24601 catcaatata taggtaattc aaaatattta tttacttact tcgttaaaca taatccaatg
    24661 actgcctgtt attttgttac tgacgatcgt cgcggaccac atttcatatc acctaagtct
    24721 gaaaaagctg acgaattgat taacagcgca cgcgtagtta tagttgaaaa tgatatacct
    24781 gaaacgttac aaccaaacgg tactttaatt caattacatc aaggcacacc aataatgcaa
    24841 ttatttttag atagtaaaga gcctattaaa aatatagaaa caccttttta tcgtgctaaa
    24901 aggtataatc gctggttgca attcgattat gttatacatt ctgctgatga tattagccac
    24961 ttttatcaaa cggcttttcc tagtcaccaa gcaaatgtat tggcttatgg taatcctaaa
    25021 caccaatatt tactacaaaa gcgtaacgaa agtactactc aacaacagta taagaaatcg
    25081 tttaaaataa atgatcaaaa accagtttta ttatacgcac ctattggatt agtgagtgcg
    25141 caacaattac cactatcaga tgctttattc aaagcctatc atgttgtggt tcagggtgtt
    25201 gatgaagcga tcttaccaga agaggcgctt gttgcaccaa agtatcttag tgctcaagac
    25261 ttaattctaa tgtctgatgt cgttatcact gattactcaa atataatatt tgatgcgatg
    25321 gctatagaca aaacagtagc attatacact ccaaatcaca gtcaatacat tgagtctcaa
    25381 ggtgttaacg aagatatttg gcgtcatcta tcaaaaattt ggtatacaga ccgacaatta
    25441 ttaattaata atttaatttc ccaggcaata ccagtgatta aatatccaca aatacaacat
    25501 aaagaacaac cattagaatc tatttcacaa cttatattgt ctaagatgac atctaatcaa
    25561 tagtgtttaa atttctcagt ggctgtgaat gaggtttaaa agtgctataa aacgtaaact
    25621 tttatacttt aaaatacgca aaaaacggta aaccctaatt catattatag agtttaccgt
    25681 tttatttttt taacttgcat catagttata ttaacattat tgttggtagt ttggatcagt
    25741 aaccattgct tgtccagtat aatcaaccgt tacaattgaa tattttccat ttgcatttgg
    25801 gtctttaaaa ctaaacacat acttatagtt gccattatgt tcttcaatag aataatcatt
    25861 atacacttta ttattactac caaatttatt tgcttcatta ttagccgcat ttaaagctgt
    25921 ttggaaattt ggcaattgct gtaaagcttg agttttattt ccattaaacg gataaatttg
    25981 acgtgcaacc ggcgcggcat tttgtccata atatggtgca acgtaacttg atttttgatt
    26041 attacttgat tggttattat ttgtttggtt ttggtcattg tttgttgcat ttgaattaga
    26101 ttgttgctgg ttatcgtttg cactattatc tttattatct ttgtttacgt ctttactatc
    26161 atctttattg tctttcttat ctttagatga atcatttgtt tttttatctt gttgttcagt
    26221 tttcgcttta tcatcttttt ctttattact gtctttttgt tggtcactat cttgaccaca
    26281 tgcagctaaa aataatgata atgctagtaa ccctgtaact aatcttttca tacatatctc
    26341 ctcctataat tcgatattca ttgaataagc ttgaaataca tatctaccat gtgtatcatt
    26401 tcatggcttg ttaccaaaga cgtcaacatt aatatgattt gaattatcac aatacccata
    26461 actacaatct aaggttcttt ttattatacc ctaatttttg ttcattatta tttaattttt
    26521 gtgaatttta tgttttctat aaatttaatt attttacttt aacaattcat tacgcattta
    26581 gcatttcaag gcatacacaa tatttattac tatgatttca ttttatctgc tgcaaaaaca
    26641 atcattataa ctctttttcc ataattaaat ctgtatccgt tacatcacct gtttgaaaat
    26701 gatgttcacc aaccacttta aatccatgac gtttataaaa tgcttgagca cgaggattat
    26761 gctcccaaac tcctagccaa attttatgtt tattatgttc ttgagcaatt ttttcggcca
    26821 attctatcaa ttgtgaacct cttccgccac cttgaaagtc tttcaaaaaa tatatgcgct
    26881 gcacttctaa ataggtctcc cccatttctt cagtttgagc actattaata ttcatcttta
    26941 tataaccaac attcgcacca tcttcttgat aaaaataatg aaatgaatct acatggttaa
    27001 tctcttgtgt aaatttttct acagtataat tgtctttaaa aaattgatca aaatctttgt
    27061 catcatagta agaaccaaac gtgtcataaa atgttctagt tgctaattca actaattcac
    27121 tagcattttg ttctgaaatt tctttgatta tcccagccat ataaatcctc caataaacag
    27181 tgatcgaatc aaaatattac ttatgttatt tttcagccaa aactatttaa aaatacatta
    27241 acacaaatca attacaaatt gtattgattg tgtgtaacat caataaatga tacatttatt
    27301 ccagtaaaat ggccgtattt tcaaaagaga aaaagagagg atgtatcgtt gtgatagaaa
    27361 catttaaagc gtttgtaatt gataaagatg agagcggtaa agtgacacca actttcaaac
    27421 aattatcgcc tactgattta cctaaaggag atgtgctgat taaagtacat tactctggta
    27481 taaattataa agatgcttta gcgactcaag atcataatgc agtcgtaaaa tcgtatccta
    27541 tgattccagg aatagattta gctggaacaa ttgttgaatc cgaagcacca ggctttgaaa
    27601 aaggagaaca agtaattgta acgagttatg acctaggtgt tagccattat ggcggtttta
    27661 gtgaatatgc gcgtgtaaaa tcagaatgga ttatcaagct tcctgatact ttaacattag
    27721 aagaatcaat gatatatggc acagctggtt atactgccgg tttagcaatt gaaagacttg
    27781 aaaaagttgg aatgaatatt gaagatggtc ctgtactcgt tcgcggtgct tcaggtggtg
    27841 tcggtacttt agcagtactc atgcttaatg aacttggtta taaagttatc gcaagtacag
    27901 gtaaacaaga tgttagcgat caattacttg aacttggtgc caaagaagtt atcgatcgac
    27961 ttcctgttga agatgatcat aaaaagccac tcgcatcatc aacttggcaa gcttgtgtag
    28021 accctgttgg tggcgaaggt attaattatg ttacaaaacg tttaaatcat agtggatcaa
    28081 ttgcagttat tggtatgact gccggcaata cttatactaa ttctgtattc cctcacattt
    28141 taagaggcgt aaacatttta ggaattgact cagtatttac tgctatgaaa ttaagacagc
    28201 gcgtttggcg tcgtctcgca aaagatttaa agcctgaaaa tttacatgag atcaagcaag
    28261 ttattacatt tgatgaactt ccagaacaac ttaacaaagt aattaatcat gaaaataaag
    28321 gccgcattgt tatcgatttc ggtgtagata aatagtattc atgaaaaaga catcccgtta
    28381 tgcgagatgt cttttttaat ttagtatttg atatacatac cgcctgaatc tggttcggta
    28441 ggcataaatc caaattttgc atataattta tccgctgggt agtctgcgat cagactaacg
    28501 tatgcacttt cgacagatac atttttaata tacttcataa tatgttccat aattagactg
    28561 ccgtaatctt gaccttgata acttttcgaa acagcaatat caacaatttg aaaaacagtt
    28621 ccgccatcgc caatcactct acccatacca attaaccgat ctttatcata caaggttact
    28681 gtaaataagg cattaggtaa tcctttttca gctgcttcgc gcgtctttgg actcatacct
    28741 gcgttaatcc ttaatgcgca ataatcctcg caagtcggaa tatcatatgt cactttaacc
    28801 attatttacc ccacttttca tcacacaata tatcaaccta gtataaatgt ttatttacaa
    28861 tagtcttatt cgcttcttta aacacttcat gatgacttga aacataaccc tctgcattcg
    28921 catctggttg gatatatgtt ttagcaaggt tcgctgcatt tgcaccatca ctaaatgcac
    28981 ttgcaattag atgtgatttt gcatcatgat aaacaatatc tccacacgca tagataccag
    29041 gtatactagt tgtcgtatta ccaaatcctt taacacgaca atcatcatgc atatctagct
    29101 ttgaagatgt ttcactcaat aatgtattac aacgatcaaa cccatgacta ataatgacat
    29161 cgtcaaattt aactgtatgc ctatcgccac tttcaacatg ttccaaaaca acttcactta
    29221 tatgcgtttc atcatcattg ccgaccaagt atttaatacg tgtttttggg catagtttca
    29281 catttaaatc tgtcaccaac gttttcatcg cttcatgacc acttacatct tcttttcgat
    29341 aaacaactgt cacgctttta gcaatcttgg caatatcatg cgcccaatct aatgctgtat
    29401 ttcctccacc tgatattaat acatctttat ctttgaaacg tctgtaactt tgtacaacat
    29461 aatgtaaatt agttaattga tatctctcta cacctttaac atctaattgt tttggattaa
    29521 taatacccgc accaattgca atgataactg ctttcgatgt atatatttct cccgcttctg
    29581 tttcaacttc gaaatgacgt tctgcctttt tcctaatatc taccacacgt tcattcaaat
    29641 gaacttccgg tttaaaacat aatccttgct taattgtatc ttttaaaatt tcatgacaag
    29701 gttttggcgc aatgccgcca atatcccaaa taattttttc agggtaaatt ctcatcttac
    29761 cccctaattc agattgaaca tctatcaatc ttacagacat atctcgcaat ccagcataaa
    29821 agcttgcata caaaccagac ggaccgccac caatgattgt aacatctttc attatgtgcc
    29881 tcctatgact ctctatattc atttctttca ttaacgtgct caaattgata attattatca
    29941 tttaaagcca ttatactatt aatatttata ttgttaaaat aaatcgcata gttaatcatg
    30001 aattatcaat aactatccca tcacaatata aaaacacacc tttcaaagta agaaacaaca
    30061 atgttccttt gaaagatgtg tatatttttt agttctagtt atattatttt ttaaaagact
    30121 catcacgtgg ttctttaaga attgcttgtc ttaaaaggaa aaatagcaac aataaacctg
    30181 caagcatacc tgtgtgccca atacctgcaa agcctgcaaa tgcttctgga gagtatgatt
    30241 taccagtgac ttggaagaat ccttttgtca ccatcatacc tatagtaacg ataacaccta
    30301 tgttatatac atagaagaac cagttaaata agtagtaact tgataattta aataacttct
    30361 ccaatggtaa taatattaaa aacataaaca tacctaaaat aagtgtatgt gtgtgtacaa
    30421 gtgataattg tgttgtacca gtaaaatcat atgcttttgt tagctctcta tagaaaaagc
    30481 cactaaataa acctatgatc atataaaaaa gaaatgaata taataaccta cgcattttga
    30541 atcctccctc accttttcat tatatccgat tatacctatt ataacagtga caaaaaagcg
    30601 aagatatgac acaactgaat ttggtttagc acatagtatg acagctaaaa taactttaca
    30661 tcaagcgcta tacaaataaa atagctatac catgacgctc gctacaaacg tcttgtatag
    30721 ctattatttc tatttattaa attatgacgg cacttcagta cgttttgtgc tatgttttct
    30781 aacaatagct gaaactaaac ttgatacagc gccaaaaatc ataaacccta taaacatcca
    30841 cattgtacta tttaattcaa tatgatgatt caagtataat agttctctta cacttgttgc
    30901 atagtgtgtg aatggattcc aatcatatac atatttttga taactttcag gcaacatttg
    30961 tttaggtaac gttacaagtt gcatactaaa gaacattaaa atgaagaaaa ttggaactga
    31021 cttcatacct agccatacca taacacctaa aataaggccc acgaaaccaa gaatcgcaaa
    31081 tgctacaaaa attgcgatac gatttggatg gtcaaaatca aatccttgaa cgccttgcat
    31141 gaaataaata taaacaaagc tacctgcaaa tgctgcaaca actgcaaata tcatctgtcc
    31201 aattgaagca atgatacgat gttgcacgac gatattgtta ctagttctaa atgcaaagaa
    31261 caataagata gacgttacga ttgaacctat ccaaattggc ataaacatta ggaatggtgc
    31321 attaccacct gcttggtgat ctttaacttt attaagtttt tcattatcca cttttactgg
    31381 attcgtcaaa ccattgatgt ccgcggcatt gactttaaca ttttgactcg ttaatgtttc
    31441 caaactttgc ttcgtaattt gagcattaat attatcaccc ataccagtta acacagctga
    31501 tgcaatttgt gaaccttgta agcttgatcc ttcacttaca atcgttttaa atttagcctg
    31561 tttaacctct acttgttgat tccccatttt ttgtttcatt tgttgaacca cttgtggcgg
    31621 aatttcacct gaagcaactt tttgttgcat ttcttctttt ttactatcca taactacttt
    31681 ttgtgtttta ctcatagcat cttttgaaaa gtctttatca ataatagcta ctccaaagat
    31741 tttttgatct ttcaaatctt tttcagggtc tttttcacta tcaaccttaa cccatttaat
    31801 tttattagaa tcactatcta ataatttatc ctctaatttt ttaccaatgt taacattttt
    31861 atcttgaatc gttgtacctt tatcctcgtt taatatacca attggtaaat cttttggttt
    31921 aggattatat gcaggataaa aagctaaaga aaagattacc aagataatca tagttgctat
    31981 tggcgcaatc cataataatt tatttttaaa tatattcatt ttattaatta cctccttatt
    32041 agacaatgtg ttgaatattt cgcatgttgt ctattataat atgtaagtgg gaataaacaa
    32101 tacacaatgt tcatcaatac gttcattaat agacaaatac acccacattg ttcaataaca
    32161 caaggaggtt gatttatttt tgaaagaaga taggcgaatt agaaagacaa agtcctccat
    32221 taaacaagcg ttcactaaat tactacaaga aaaagattta gaaaagatta ccattcgcga
    32281 cataacaaca cgcgctgata tcaatagagg gacattttac ttacattacg aggataaata
    32341 tatgttactc gcagatatgg aagatgagta tatttcagaa ctaacgacat atactcaatt
    32401 tgatttgtta cgcggttctt caattgaaga cattgcgaat acttttgtaa ataatatact
    32461 caaaaatatt ttccaacata ttcatgataa tttagagttt tatcatacta ttttacaatt
    32521 ggaacgcacg agtcaattag aattgaaaat caacgaacat atcaaaaata atatgcaacg
    32581 ttatattagt atcaatcatt ctatcggagg cgttccagag atgtattttt acagttacgt
    32641 ttccggagca acaatttcaa ttattaaata ctgggtaatg gacaaacagc ccatttcagt
    32701 tgatgaatta gctaaacatg tgcataatat tatttttaat ggtccattaa gaataatggc
    32761 agaaaaccgt ttgcataaat caaaccttga ttcactaact taagtaatac tcgcttttcc
    32821 taaatccttt agaaaggaac tatatatcat gattacttca ttcagacatt ctgaagatat
    32881 agataaacat attataaaaa caccattaga tcatacagcg tcatggatta atgtagtaga
    32941 accagaccga gaagaaattg aaaatcttat ggaacaatat aatatacctg aagactttat
    33001 acgtgaccct ttggactcag aagaaagttc ccgtattgaa tatgacgaag atactggtta
    33061 ctcattaatc attattgatt tacctatcgt caattcaact aatcgtagcg ttctatcttt
    33121 tgtaacgatt ccattaggca ttattattgg caacggtatt atcgtaacag tttgtgacgc
    33181 tgaaaatgaa tttttagaaa atttacctaa gcgtgatatt aatttaaaat ttcacagcag
    33241 atttgcatta gaaattttaa ctactattgc cgaccattat aatcgtaact tacgattact
    33301 taataaaagt agaattcgta ttgagaaaga actaaaaaat aacattacta acaaacaact
    33361 tttcaaatta atggaagttg aaaaaagttt agtatacttt ttagctgcct taaaaggtaa
    33421 cgatacaatt attaaaaagt tattccgttt acctgcaatt aaacgttttg aagaagatga
    33481 agaattacta gaagatttaa tcattgaaaa taaccaagcc atcgaaacaa cagaattaca
    33541 tcaacgtatc ttagaaagta tcacaacatc atacgcttcc ttattatcta atgatatgaa
    33601 tacgattatg aagacattaa cacttttcac ggtactatta acgttaccaa cgctcgtatt
    33661 tagtttcttc ggtatgaatg tgccgttgcc aattgatgac catagttata tatcttggat
    33721 tattgtggta ggtatttcat taattcttgt cgtaattgtt agtattttct tatggcgtaa
    33781 acagaaatta taactaaatt aaaaggtatg acagcttaaa gtttcatcct attgcatttg
    33841 caatgactga tactttgatt actgtcatac ctctttttta ttctacaact tctttatttg
    33901 cttttctttt agataaaatt aaagtaactg ttatagcaat gatgaatgat attgtcatac
    33961 caacaaagta gtgtaaccat cctgcatgta ctggattgat tgaaataaat cctggcaatc
    34021 cagcagttcc taatgcgatt gctttaacct tgaagaaagc aatatatgct gaaccaatac
    34081 ctgatccaac gatagcgcca ataaatggat atcttagttt taagttaaca ccaaacatag
    34141 ccggttctgt aataccaagt aatgctgaaa tacctgcggc agatgccaca ccttttaact
    34201 tcttattttg tttaataata aagaacgctg caattgctgc accaccttgt gcaacattag
    34261 acattgtcgc aatcgggaat ataaatgatc cacccgtttt agtggcatct gcaattaatg
    34321 tcgtttctac agctataaag ctatgatgca tacctgtaat aacaatcgga gcatacaata
    34381 atccgaatat taatccacca attgcaccac caaattcata aagccatgtt aaaccatctg
    34441 ataaccagta acctaattgt cgagtgattg gacctacaaa tgaaaatgtt agaaatgctg
    34501 ttataaaaat agataacaat ggtgttaaca aattatctaa caccgttgga ataactttgc
    34561 gtaacccttt ttcaattgag gctaagatat aagctgctac aagcataggt aacacttgtc
    34621 cttgataacc tacttgatta atatgcaaac caaaaacatc ccaatatgga atagccttac
    34681 ctgcttcaac tgcttttggg aaatcgtatg cgctcattag cgatggatga actagtatca
    34741 tacctaatac agcacctaaa aatggattgc caccaaagcg ttttgctgca ctaaatccaa
    34801 ttaaaattgg taataatgta aatggtgcat tcgcaaaaac atttatcatt tcagctaatc
    34861 cagcaaattg actatataca tctatcaatg atttacctga aaagaataaa tctttcgcag
    34921 tcaaaatgtt atttaacccc attaataaac caccagcaac gatggctggt ataatcggaa
    34981 caaagatatc tgaaagcatt tttacaaatc gctgtaacgg attcatattt ttagcagatt
    35041 gtgctttgac ttccgaagtg gttgatgctt cttttccagt taatttttcc agttcactaa
    35101 atactttatt gactgtacca gacccaataa taatttggta ttgtccccca gtagaaaacg
    35161 tccctttaac tacatccata ttgtttagcg cctcttcatt tactaaactt tcatcattta
    35221 aaactaatcg tagtctcgtt gcacaatgcg ccattgcatc taaattctct tctccgccta
    35281 tcgcgttcaa aatatcttct gcggattgct tataattcat atatttagcc tcctaattag
    35341 aaccggttcc atttattcca ctttattgta accggttcca aaaatatttg caacactcta
    35401 ttatcatttt atataaacac ctacaccatt tttgatagcg cattcatttt tattgctata
    35461 ctatatgcat gtatattaac attaattatc ggaggtaatt ttatgaaaga acgtggccat
    35521 atcgaacagc tttggcgtga ggaaaaatat catgtattgt tacacagtca acagtcttac
    35581 caaatgataa ggaatgcttt aaaaacggat ttatcacttc atcaagtcca acaaatgatt
    35641 gatgatgctt tgttaattga accgagtata ggaagtgtat gtaatgcttt tgatcatatg
    35701 tggggatatt ttaaaaaatg tgcgaatgaa gaagaaagaa aacaatcaaa actacttaaa
    35761 gctgatttta ttaatggtaa aatcgacacc caaactttat tagatttttt agcagaactt
    35821 gctaataaat atgatgttca atatttacta caaagtcgtg ttttaaacac aaaaagaaag
    35881 agatagattg aaactttagt tgtaacggac aaatagtagt cattggagtt cagcaattca
    35941 tactttgtac taaagtttac tataatctat ctctcaaggt ttaaatgtac atatctatat
    36001 taagatgcgt ttcatgatgt tagaagatac catacacatt taattagtta cagcataaat
    36061 caat
//
LOCUS       DAHHSC010000007        36061 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.007, whole genome shotgun sequence.
ACCESSION   DAHHSC010000007 DAHHSC010000000
VERSION     DAHHSC010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 36061)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36061)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36061
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.007"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            173..32047
                     /gene="ebh"
                     /locus_tag="NBI57_000294"
     CDS             173..32047
                     /gene="ebh"
                     /locus_tag="NBI57_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001635093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hyperosmolarity resistance protein Ebh"
                     /protein_id="HCD1821281.1"
                     /translation="MNYRDKIQKFSIRKYTVGTFSTVIATLVFLGFNTSQAHAAETNQ
                     PASVVKQKQQSNNEQTENRESQVQNSQNSQNSQSLSATHENEQPNNSQANLVNQKVAQ
                     SSTTNDEQPASQNVNTKKDSATAATTQPDKEESKHKQNESQSANKNGNDNRAAHVENH
                     EANVVTASDSSDNGNVQHDRNELQAFFDANYHDYRFIDRENADSGTFNYVKGIFDKIN
                     TLLGSNDPINNKDLQLAYKELEQAVALIRTMPQRQQTSRRSNRIQTRSVESRAAEPRS
                     VSDYQNANSSYYVENANDGSGYPVGTYINASSKGAPYNLPTTPWNTLKASDSKEIALM
                     TAKQTGDGYQWVIKFNKGHAPHQNMIFWFALPADQVPVGRTDFVTVNSDGTNVQWSHG
                     AGAGANKPLQQMWEYGVNDPDRSHDFKIRNRSGQVIYSWPTVHVYSLEDLSRASDYFS
                     EAGATPATKAFGRQNFEYINGQKPAESPGVPKVYTFIGQGDASYTISFKTQGPTVNKL
                     YYAAGGRALEYNQLFMYSQLYVESTQDHQQRLNGLRQVVNRTYRIGTTKRVEVSQGNV
                     QTKKVLESTNLNIDDFVDDPLSYVKTPSNKVLGFYPTNANTNAFRPGGVQELNEYQLS
                     QLFTDQKLQEAARTRNPIRLMIGFDYPDGYGNSETLVPVNLTVLPEIQHNIKFFKNDD
                     TQNIAEKPFSKQAGHPVFYVYAGNQGNASVNLGGSVTSIQPLRINLTSNENFTDKDWQ
                     ITGIPRTLHIKNSTNRTNNARERNIELVGNLLPGDYFGTIRFGRKEQLFEIRVKPHTP
                     TITTTAEQLRGTALQKVPVNISGIPLDPSALVYLVAPTNQTTNGGSEADQIPSGYTIL
                     ATGTPDGVHNTITIRPQDYVVFIPPVGKQIRAVVYYNKVVASNMSNAVTILPDDIPPT
                     INNPVGINAKYYRGDEVNFTMGVSDRHSGIKNTTITTLPSGWTSNLTKSDNKNGSLAI
                     TGRVSMNQAFNSDITFKVSATDNVNNTTNDSQSKHVSIHVGKISEDAHPIVLGNTEKV
                     VVVNPTAVSNDEKQSIITAFMNKNQNIRGYLASTDPVTVDNNGNVTLHYRDGSSTTLD
                     ATNVMTYEPVVKSEYQTANAAKTATVTIAKGQSFNIGDIKQYFTLSNGQAIPNGTFTN
                     ITSDRTIPTAQEVSQMNAGTQLYHIVASNAYHKDTEDFYISLKIVDVKQPEGDQRVYR
                     TSTYDLTTDEISKVKQAFINANRDVITLAEGDISVTNTPNGANVSTITVNINKGRLTK
                     SFASNLANMNFLRWVNFPQDYTVTWTNAKIANRPTDGGLSWSDDHKSLIYRYDATLGT
                     QITTNDILTMLKATTTVPGLRNNITGNEKAQAEAGGRPNYRTTGYSQSNATTDGQRQF
                     TLNGQVIQILDIINPSNGYGGQPVTNSNTRANHSNSTVVNVNEPAANGAGAFTIDHVV
                     KSNSTHNASDAVYKAQLYLTPYGPKQYVEHLNQNTGNTTDAINIYFVPSDLVNPTISV
                     GNYTNHQVFSGETFTNTITANDNFGVQSVTVPNTSQITGTVDNNHQHVSATAPNVTSA
                     TSKTINLLATDTSGNTATTSFNVTVKPLRDKYRVGTSSTAANPVRIANISNNATVSQA
                     DQTTIINSLTFTSNAPNRNYATASANEITSKTVSNVSRTGNNANVTVTVTHQDGTTST
                     VTVPVKHVIPEIVAHSHYTVQGQDFPAGNGSSAADYFKLSNGSAIPDATITWVSGQAP
                     NKDNTRIGEDITVTAHILIDGETTPITKTATYKVVRTVPKHVFETARGVLYPGVSDMY
                     DAKQYVKPVNNSWSTNAQHMNFQFVGTYGPNKDVVGISTRLIRVTYDNRQTEDLTILS
                     KVKPDPPRIDANSVTYKAGLTNQEIKVNNVLNNSSVKLFKADNTPLNVTNITHGSGFS
                     SVVTVSDALPNGGIKAKSSISMNNVTYTTQDEHGQVVTVTRNESVDSNDSASVTVTPQ
                     LQATTEGAVFIKGGDGFDFGHVERFIQNPPHGATVAWHDSPDTWKNTVGNTHKTAVVT
                     LPSGQGTRNVEVPVKVYPVANAKAPSRDVKGQNLTHGTNAIDYITFDPNTNTNGITAA
                     WANRQQPNNQQAGVQHLNVDVTYPGISAAKRVPVTVNVYQFEFPQTTYTTTVGGTLAS
                     GTQASGYAHMQNASGLPTDGFTYKWNRDTTGTNDANWAAMNKPNTAQVVNAKYDVIYN
                     GHTFATSLPAKFVVKDVQPAKPTVTETAAGAITIAPGANQTVNTHAGNVTTYADKLVI
                     KRNGNVVTTFTRRNNTSPWVKEASADNVTGIVGTNNGITVAAGTFNPADTIQVVATQG
                     SGETISDEQRSDDFTVVAPQPNQATTKIWQNGHIDITPNNPSGHLINPTQAMDIAYTE
                     KVGNGAEHSKTINVVRGQNNQWTIANKPDYVTLDAQTGKVTFNANTIKPNSSITITPK
                     AGTGHSVSSNPSTLTAPAAHTVNTTEIVKDYGSNVTAAEINNAVQVANKRTATIKNGT
                     AMPTNLAGGSTTTIPVTVTYNDGSTEEVQESIFTKADKRELITAKNHLDDPVSTEGKK
                     PGTITQYNNAMHNAQQQINTAKTEAQQVINNERATPQQVSDALTKVRAAQTKIDQAKA
                     LLQNKEDNSQLVTSKNNLQSSVNQVPSTAGMTQQSIDNYNAKKREAETEITAAQRVID
                     NGDATAQQISDEKHRVDNALTALNQAKHDLTADTHALEQAVQQLNRTGTTTGKKPASI
                     TAYNNSIRALQSDLTSAKNSANAIIQKPIRTVQEVQSALTNVNRVNERLTQAINQLVP
                     LADNSALRTAKTKLDEEINKSVTTDGMTQSSIQAYENAKRAGQTETTNAQNVINNGDA
                     TDQQIAAEKTKVEEKYNSLKQAIAGLTPDLAPLQTAKTQLQNDIDQPTSTTGMTSASV
                     AAFNDKLSAARTKIQEIDRVLASHPDVATIRQNVTAANAAKTALDQARNGLTVDKAPL
                     ENAKNQLQHSIDTQTSTTGMTQDSINAYNAKLTAARNKVQQINQVLAGSPTVDQINTN
                     TSAANQAKSDLDHARQALTPDKAPLQNAKTQLEQSINQPTDTTGMTTASLNAYNQKLQ
                     AARQKLTEINQVLNGNPTVQNINDKVAEANQAKDQLNTARQGLTLDRQPALTTLHGAS
                     NLNQAQQNNFTQQINAAQNHAALETIKSNITALNTAMTKLKDSVADNNTIKSGQNYTD
                     ATPANKQAYDNAVNAAKGVIGETTNPTMDVNTVNQKAASVKSTKDALDGQQNLQRAKT
                     EATNAITHASDLNQAQKNALTQQVNSAQNVQAVNDIKQTTQSLNTAMTGLKRGVANHN
                     QVVQSDNYVNADTNKKNDYNNAYNHANDIINGNAQHPVITPSDVNNALSNVTSKEHAL
                     NGEAKLNAAKQEANTALGHLNNLNNVQRQNLQSQINGAHQIDAVNTIKQNATNLNSAM
                     GNLRQAVADKDQVKRTEDYADADTAKQNAYNSAVSSAETIINQTANPTMSVDDVNRAT
                     SAVTTNKNALNGDEKLVQSKTDAARAIDALPHLNNAQKADVKSKINAASNIAGVNTVK
                     QQGTDLNTAMGNLQGAINDEQTTLNSQNYQDATPSKKTAYTNAVQAAKDILNKSNGQN
                     KTKDQVTEAMNQVNSAKNNLDGTRLLDQAKQTAKQQLNNMTHLTTAQKTNLTNQINSG
                     TTVAGVHTVQSNANTLDQAMNTLRQSIANNDATKASEDYVDANNDKQTAYNNAVAAAE
                     TIINANSNPEMNPSTITQKAEQVNSSKTALNGDENLATAKQNAKTYLNTLTSITDAQK
                     NNLISQISSATRVSGVDTVKQNAQHLDQAMANLQNGINNESQVKSSEKYRDADTNKQQ
                     EYDNAITAAKAILNKSTGPNTAQNAVEAALQRVNTAKDALNGDAKLIAAQNAAKQHLG
                     TLTHITTAQRNDLTNQISQATNLAGVESVKQSANSLDGAMGNLQTAINDKSGTLASQN
                     FLDADEQKRNAYNQAISAAETILNKQTGPNTAKTAVEQALNNVNSAKHALNGTQNLNN
                     AKQAAITAINGASDLNQKQKDALKAQANGAQRVSNANDVQRNATELNTAMGQLQHAIA
                     DKTNTLASSKYVNADSTKQNAYTTKVTNAEHIISGTPTVVTTPSEVTAAANQVNSAKQ
                     ELNGDERLRVAKQNANTAIDALTQLNTPQKAKLKEQVGQANRLEDVQSVQTNGQSLNN
                     AMKGLRDSIANETTVKASQNYTDASPNNQSTYNSAVSNAKGIINQTNNPTMDTSAITQ
                     ATTQVNNAKNGLNGAENLRNAQNTAKQNLNTLSHLTNNQKSAISSQIDRAGHVSEVTA
                     AKNAATELNAQMGNLEQAIHDQNTVKQGVNFTDADKAKRDAYTNAVSRAETILNKTQG
                     ANTSKQDVEAAIQNVTSAKNALNGDQNVTNAKNAAKNALNNLTSINNAQKRDLTTKID
                     QATTVAGVEAVSNTGTQLNTAMANLQNGINDKANTLASENYHDADSDKKTAYTQAVTN
                     AENILNKNSGSNLDKAAVENALSQVTNAKGALNGNHNLEQAKSNANTTINGLQHLTTA
                     QKDKLKQQVQQAQNVAGVDTVKSSANTLNGAMGTLRNSIQDNTATKNGQNYLDATERN
                     KTNYNNAVDSANGVINATSNPNMDANAINQIATQVTSTKNALDGTHNLTQAKQTATNA
                     IDGATNLNKAQKDALKAQVTSAQRVANVTSIQQTANELNTAMGQLQHGIDDENATKQT
                     QKYRDAEQSKKTAYDQAVAAAKAILNKQTGSNSDKAAVDRALQQVTSTKDALNGDAKL
                     AEAKAAARQNLGTLNHITNAQRTALEGQINQATTVDGVNTVKTNANTLDGAMNSLQGA
                     INDKDATLRNQNYLDADESKRNAYTQAVTAAEGILNKQTGGNTSKADVDNALNAVTRA
                     KAALNGAENLRNAKTSATNTINGLPNLTQLQKDNLKHQVEQAQNVVGVNGVKDKGNTL
                     NTAMGALRTSIQNDNTTKTSQNYLDASDSNKNNYNTAVNNANGVINATNNPNMDANAI
                     NDMANQVNTTKAALNGAQNLAQAKTNATNTINNAQDLNQKQKDALKTQVNNAQRVSDA
                     NNVQHTATELNGAMTALKAAIADKERTKASGNYVNADQEKRQAYDSKVTNAENIINGT
                     PNATLTVNDVNSAASQVNAAKTALNGDNNLRVAKEHANNTIDGLAQLNNVQKAKLKEQ
                     VQSATTLDGVQTVKNSSQTLNTAMKGLRDSIANEATIKAGQNYTDASPNNRNEYDSAV
                     TAAKAIINQTSNPTMEPNTITQATSQVTTKEHALNGAQNLAQAKTTAKNNLNNLTSIN
                     NAQKDALTRNIDGATTVAGVNQETAKATELNNAMHSLQNGINDETQTKQTQKYLDAEP
                     SKKSAYDQAVNAAKAILTKASGQNVDKAAVEQALQNVNSTKTALNGDAKLNEAKAAAK
                     QTLGTLTHINNAQRNALDNEITQATNVEGVNTVKAKAQQLDGAMGQLETSIRDKDTTL
                     QSQNYQDADDAKRTAYSQAVNAAATILNKTAGGNTPKADVERAMQAVTQANTALNGIQ
                     NLERAKQAANTAITNASDLNTKQKEALKAQVTSAGRVSAANGVEHTATELNTAMTALK
                     RAIADKADTKASGNYVNADANKRQAYDEKVTAAEHIVSGTPTPTLTPSDVTNAATQVT
                     NAKTQLNGNHNLEVAKQNANTAIDGLTSLNGPQKAKLKEQVGQATTLPNVQTVRDNAQ
                     TLNTAMKGLRDSIANEATIKAGQNYTDASQNKQNDYNNAVTAAKAIIGQTTSPSMIAQ
                     EINQAKDQVTAKQQALNGQENLRTAQTNAKQHLNGLSDLTNAQKDAAKRQIEGATHVN
                     EVTQAQNNADALNTAMTNLKNGIQDQNTIKQGVNFTDADEAKRNAYTNAVTQAEQILN
                     KAQGPNTAKDGVETALQNVQRAKNELNGNQNVANAKTTAKNALNNLTSINNAQKAALK
                     SQIEGATTVAGVNQVSTMASELNTAMSNLQRGINDEAATKAAQKYTEADRDKQTAYND
                     AVTAAKTLLDKTAGSNDNKVAVEQALQRVNTAKTALNGDARLNEAKNTAKQQLATMSH
                     LTNAQKANLTEQIERGTTVAGVQGIQANAGTLNQAMNQLRQSIASKDATKSSEDYQDA
                     NADLQNAYNDAVTNAEGIISATNNPEMNPDTINQKASQVNSAKSALNGDEKLAAAKQT
                     AKSDIGRLTDLNNAQRTAANAEVDQAPNLAAVTAAKNKATSLNTAMGNLKHALAEKDN
                     TKRSVNYTDADQPKQQAYDTAVTQAEAITNANGSNANETQVQAALNQLNQAKNDLNGD
                     NKVAQAKETAKRALASYSNLNNAQSTAATSQIDNATTVADVTAAQNTANELNTAMGQL
                     QNGINDQNTVKQQVNFTDADQGKKDAYTNAVTNAQGILDKANGQNMTKAQVEAALNQV
                     TTAKNALNGDANVRQAKSDAKANLGTLTHLNNAQKQDLTSQIEGATTVNGVNSVKTKA
                     QDLDGAMQRLESAIANKDQTKASENYIDADPTKKTAFDNAITQAESYLNKDHGTNKDK
                     QAVEQAIQSVTSTENALNGDANLQCAKTEATQAIDNLTQLNTPQKTALKQQVNAAQRV
                     SGVTDLKNSATSLNNAMDQLKQAIGDHDTIVAGGNYTNASPDKQGAYTDAYNAAKNIV
                     NGSPNVITNAADVTAATQRVNNAETSLNGDTNLATAKQQAKDALRQMTHLSDAQKQSI
                     TGQIDSATQVTGVQSVKDNATNLDNAMNQLRNSIANKDEVKASQPYVDADTDKQNAYN
                     TAVTSAENIINATSQPTLDPSAVTQAANQVNTNKTALNGAQNLANKKQETTANINRLS
                     HLNNAQKQDLNTQVTNAPNISTVNQVKTKAEQLDQAMERLINGIQDKDQVKQSVNFTD
                     ADPEKQTAYNNAVTAAENIINQANGTNANQSQVEAALSTVTTTKQALNGDRKVTDAKN
                     NANQTLSTLDNLNNAQKGAVTGNINQAHTVAEVTQAIQTAQELNTAMGNLKNSLNDKD
                     TTLGSQNFADADPEKKNAYNEAVRNAENILNKSTGTNVPKDQVEAAMNQVNTTKAALN
                     GTQNLEKAKQHANTAIDGLSHLTNAQKEALKQLVQQSTTVAEAQGNEQKANNVDAAMD
                     KLRQSIADNATTKQNQNYTDASPNKKDAYNNAVTTAQGIIDQTTNPSLDPTVINQAAG
                     QVSTSKNALNGNENLEAVKQQATQSLGSLDNLNNAQKQAVTNQINGAHTVDEANQIKQ
                     NAQNLNTAMGNLKQAIADKDATKATVNFTDADQAKQQAYNTAVTNAENIISKANGGNA
                     TQTEVEQAIQQVNAAKQALNGNANVQHAKDEATALINNSNDLNQAQKDALKQQVQNAT
                     TVAGVNNVKQTAQELNNAMTQLKQGVADKEQTKADGNFVNADSDKQNAYNQAVAKAEA
                     LISGTPDVVVTPSEITAALNKVTQAKNDLNGNTNLATAKQNVQHAIDQLPNLNQAQRD
                     EYSKQITQATLVPNVNAIQQAATTLNDAMTQLKQGIANKAQIKGSENYHDADTDKQTA
                     YDNAVTKAEELLKQTTNPTMDPNTIQQALTKVNDTNQALNGNQKLADAKQDAKTTLGT
                     LDHLNDAQKQALTTQVEQAPDIATVNNVKQNAQNLNNAMTNLNNALQDKTETLNSINF
                     TDADQAKKDDYTNAVSHAEGILSKANGSNASQTEVEQAMQRVNEAKQALNGNDNVQRA
                     KDAAKQVITNANDLNQAQKDALKQQVDAAQTVANVNTIKQTAQDLNQAMTQLKQGIAD
                     KDQTKANGNFVNADTDKQNAYNNAVAHAEQIISGTPNANVDPQQVAQALQQVNQAKGD
                     LNGNHNLQVAKDNANTAIDQLPNLNQPQKTALKDQVSHAELVTGVNAIKQNADALNNA
                     MGTLKQQIQANSQVPQSVDFTQADQDKQQAYNNAANQAQQIANGTPTPVLAPDTVTKA
                     VTTMNQAKDALNGDEKLAQAKQDALANLDTLRDLNQPQRDALRNQINQAQALATVEQT
                     KQNAQNVNTAMGNLKQGIANKDTVKASENYHDADVDKQTAYTNAVSQAEGIINQTTNP
                     TLNPDDITRALTQVTDAKNSLNGEAKLATEKQNAKDAVSGMTHLNDAQKQALKGQIDQ
                     SPEIATVNQVKQTATSLDQAMDQLSQAINDKDQILADGNYLNADPDKQNAYKQAVAKA
                     EALLNKQSGTNEVQAQVESITNEVNAAKQALNGNDNLANAKQQAKQQLANLTHLNDAQ
                     KQSFESQITQAPLVTDVTTINQKAQTLDHAMELLRNSVADNQTTLASEDYHDATAQRQ
                     NDYNKAVTAANNIINQTTSPTMNPDDVNGATTQVNNTKVALDGDENLAAAKQQANNRL
                     DQLDHLNNAQKQQLQSQITQSSDIAAVNGHKQTAESLNTAMGNLINAIADHQAVEQRG
                     NFINADTDKQTAYNTAVNEAAAMINKQTGQNANQTEVEQAITKVQTTLQALNGDHNLQ
                     VAKTNATQAIDALTSLNDPQKTALKDQVTAATLVTAVHQIEQNANTLNQAMHGLRQSI
                     QDNAATKANSKYINEDQPEQQNYDQAVQAANNIINEQTATLDNNAINQVAATVNTTKA
                     ALHGDVKLQNDKDHAKQTVSQLAHLNNAQKHMEDTLIDSETTRTAVKQDLTEVQALDQ
                     LMDALQQSIADKDATRASSAYVNAEPNKKQAYDEAVQNAESIIAGLNNPTINKGNVSS
                     ATQAVISSKNALDGVERLAQDKQTAGNSLNHLDQLTPAQQQALENQINNATTRDKVAE
                     IIAQAQALNEAMKALKESIKDQPQTEASSKFINEDQAQKDAYTQAVQHAKDLINKTTD
                     PTLAKSIIDQATQAVTDVKNNLHGDQKLAQDKQRATETLNNLSNLNTPQRQALENQIN
                     NAATRGEVAQKLTEAQALNQAMEALRNSIQDQQQTESGSKFINEDKPQKDAYQAAVQN
                     AKDLINQTGNPTLDKAQVEQLTHAFKQAKDNLHGDQKLADDKQHAVTDLNQLNGLNNP
                     QRQALESQINNAATRGEVAQKLAEAKALDQAMQALRNSIQDQQQTEAGSKFINEDKPQ
                     KDAYQAAVQNAKDLINQTGNPTLDKSQVEQLTQAVTTAKDNLHGDQKLARDQQQAVTT
                     VNALPNLNHAQQQTLTDAINAAPTRTEVAQHVQTATELDHAMETLKNKVDQVNTDKAQ
                     PNYTEASTDKKEAVDQALQAAQSITDPTNGSNANKDAVEQALTKLQEKVNELNGNERV
                     AEAKTQAKQTIDQLTHLNADQIATAKQNIDQATKLQPIAELVDQATQLNQSMDQLQQA
                     VNEHANVEQTIDYTQADSDKQKAYKQAIADAENVLKQNANKQQVDQALQKILNAKQAL
                     NGDERVALAKTNGKHDIDQLNALNNAQQDGFKGRIDQSNDLNQIQQIVDEAKVLNRAM
                     DQLSQEITGNEGRTKGSTNYVNADTQVKQVYDEAVDKAKQALDKSSGQNLTAEQVIKL
                     NDAVTAAKKALNGEERLNNRKAEALQRLDQLTHLNNAQRQLAIQQINNAETLNKASRA
                     INRATKLDNAMGAVQQYIDEQHLGVISSTNYINADDNLKANYDNAIANAAHELDKVQG
                     NAIAKAEAEQLKQNIIDAQNALNGDQNLANAKDKANAFVNSLNGLNQQQQDLAHKAIN
                     NADTVSDVTDIVNNQIDLNDAMETLKHLVDNEIPNAEQTVNYQNADDNAKTNFDDAKR
                     LANTLLNSDNTNVNDINGAIQAVNDAIHNLNGDQRLQDAKDKAIQSINQALANKLKEI
                     EASNATDQDKLIAKNKAEELANSIINNINKATSNQAVSQVQTAGNHAIEQVHANEIPK
                     AKIDANKDVDKQVQALIDEIDRNPNLTDKEKQALKDRINQILQQGHNDINNALTKEEI
                     EQAKAQLAQALQDIKDLVKAKEDAKQDVDKQVQALIDEIDQNPNLTDKEKQALKDRIN
                     QILQQGHNGINNAMTKEEIEQAKAQLAQALKEIKDLVKAKENAKQDVDKQVQALIDEI
                     DQNPNLTDKEKQALKDRINQILQQGHNDINNAMTKEEIEQAKAQLAQALQDIKDLVKA
                     KEDAKNAIKALANAKRDQINSNPDLTPEQKAKALKEIDEAEKRALQNVENAQTIDQLN
                     RGLNLGLDDIRNTHVWEVDEQPAVNEIFEATPEQILVNGELIVHRDDIITEQDILAHI
                     NLIDQLSAEVIDTPSTATISDSLTAKVEVTLLDGSKVIVNVPVKVVEKELSVVKQQAI
                     ESIENAAQQKIDEINNSVTLTLEQKEAAIAEVNKLKQQAIDHVNNAPDVHSVEEIQQQ
                     EQAYIEQFNPEQFTIEQAKSNAIKSIEDAIQHMIDEIKARTDLTDKEKQEAIAKLNQL
                     KEQAIQAIQRAQSISEITEQLEQFKAQMKAANPTAKELAKRKQEAISRIKDFSNEKIN
                     SIRNSEIGTADEKQAAMNQINEIVLETIRDINNAHTLQQVEAALNNGIARISAVQIVI
                     SDRAKQSSSTGNESNSHLTIGYGTANHPFNSSTIGHKKKLDEDDDIDPLHMRHFSNNF
                     GNVIKNAIGVVGISGLLASFWFFIAKRRRKEDEEEELEIRDNNKDSIKETLDDTKHLP
                     LLFAKRRRKEDEEDVTVEEKDSLNNGESLDKVKHTPFFLPKRRRKEDEEDVEVTNENT
                     DEKVLKDNEHSPLLFAKRRKDKEEDVETTTSIESKDEDVPLLLAKKKNQKDNQSKDKK
                     SASKNTSKKVAAKKKKKKSKKNKK"
     gene            complement(32106..32507)
                     /locus_tag="NBI57_000295"
     CDS             complement(32106..32507)
                     /locus_tag="NBI57_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HCD1821282.1"
                     /translation="MAKINFDAATKGNPGISTCAIVIKEDKQHYTYTHELGEMDNHTA
                     EWAACIYALEHARELNVQNALLYTDSKLIADSIEAGYVKNANFKPYFDQIEIFEKDFD
                     LLFVKWIPREQNKEANQHAQQALYKLIKKNK"
     gene            complement(32990..33694)
                     /locus_tag="NBI57_000296"
     CDS             complement(32990..33694)
                     /locus_tag="NBI57_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="queuosine precursor transporter"
                     /protein_id="HCD1821283.1"
                     /translation="MYNEILGLVTFIATFVLMVLMYRFFGKQGLIAWVAIGTIIANIQ
                     VIKTVEIFGISATLGNVMFASIYLATDILNDIYGRRVAKRAVWLGFSSTLIMIIVMQL
                     SLHFIPAPEDMAQKALHAIFDVVPRIALGSIVAYIIGQHIDVFIFSLIKKVFSSDKTF
                     FIRAYGSTFLSSIIDTALFVAIAFIGSLPGTVVFEIFITTYVLKLASTVFNVPFGYIA
                     KSFYRKGKIDKLDQGY"
     gene            33939..34133
                     /locus_tag="NBI57_000297"
     CDS             33939..34133
                     /locus_tag="NBI57_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002439757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-finger domain-containing protein"
                     /protein_id="HCD1821284.1"
                     /translation="MSRILTFSEQNALAKIDDLMNTYCNQCPIKTRLRKLEGKTKAHH
                     FCINECSIGKEIKQLGNELQ"
     gene            34145..34396
                     /locus_tag="NBI57_000298"
     CDS             34145..34396
                     /locus_tag="NBI57_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU N-terminal domain-containing protein"
                     /protein_id="HCD1821285.1"
                     /translation="MKIISISETPNHNTMKITLSESREGMTSDTYTKVDDSQPAFIND
                     ILKVEGVKSIFHVMDFISVDKENDANWETVLPKVEAVFE"
     gene            34434..35558
                     /locus_tag="NBI57_000299"
     CDS             34434..35558
                     /locus_tag="NBI57_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor C"
                     /protein_id="HCD1821286.1"
                     /translation="MEILRIEPTPSPNTMKVVLSYTREDKLSNTYKKVEETQPRFINQ
                     LLSIDGITSIFHVMNFLAVDKAPKADWEVILPDIKAAFSDANKVLESVNEPQIDNHFG
                     EIKAELLTFKGIPYQIKLTSADQELREQLPQTYVDHMTQAQTAHDNIVFMRKWLDLGN
                     RYGNIEEVMDGVLEEVLATYPESQLPVLVKHALEENHATNNYHFYRHVSLDEYHATDN
                     WKTRLRMLNHFPKPTFEDIPLLDLALSDEKVPVRRQAIVLLGMIESKEILPYLYKGLR
                     DKSPAVRRTAGDCISDLGYPEALPEMVLLLDDPQKIVRWRAAMFIFDEGNAEQLPALK
                     AHINDNAFEVKLQIEMAISRIENGDEALGSVWKQMANRTI"
     gene            35574..36011
                     /locus_tag="NBI57_000300"
     CDS             35574..36011
                     /locus_tag="NBI57_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrxA/BrxB family bacilliredoxin"
                     /protein_id="HCD1821287.1"
                     /translation="MNAYDAYMKEIAQQMRGELTQNGFTSLETSEAVSEYMNQVNADD
                     TTFVVINSTCGCAAGLARPAAVAVATQNEHRPTNTVTVFAGQDKEATATMREFIQQAP
                     SSPSYALFKGQDLVYFMPREFIEGRDINDIAMDLKDAFDENCK"
BASE COUNT        14526 a         6380 c         6453 g         8702 t
ORIGIN      
        1 ttgtaatgtg tttcaatact ttttggatag attgcgaaat tgtattgaat cgtcatcgtt
       61 ttaaattttt aaatgagaat ggaatgagca ttacaatata caagcaatca aaagtaaata
      121 cattcacaac acaacagaga cataacaaca agataaggag tgaacaatag ctgtgaatta
      181 tcgtgataaa attcaaaagt ttagtattcg taaatataca gttggtacat tttcaactgt
      241 cattgcgaca ttggtatttt taggattcaa tacatcacaa gcacatgctg ctgaaacaaa
      301 tcaaccagca agcgtggtta aacagaaaca acaaagtaat aatgaacaga ctgagaatcg
      361 agaatctcaa gtacaaaatt ctcaaaattc acaaaatagt caatcattat ccgctactca
      421 tgaaaatgag caaccaaata atagtcaagc taatttagta aatcaaaaag tagcgcaatc
      481 atctactact aatgatgaac aaccagcatc tcaaaatgta aatacaaaga aagattcggc
      541 aacggctgcg acaacacaac cagataaaga agaaagtaag cataaacaaa acgaaagtca
      601 atctgctaat aaaaatggaa acgacaatag agcggctcat gtagaaaatc atgaagcaaa
      661 tgtagtaaca gcttcagatt catctgataa tggtaacgta caacatgacc gaaatgaatt
      721 acaagcattt tttgatgcaa attatcatga ttatcgcttt attgaccgtg aaaatgcaga
      781 ttctggcaca tttaactatg taaaaggcat ttttgacaag attaatactt tattaggcag
      841 taatgatcca attaacaata aagacttgca acttgcatac aaagaattgg aacaagctgt
      901 tgctttaatt cgtacaatgc ctcaacgtca acaaactagc cgtcgatcaa acagaattca
      961 aacgcgttct gttgagtcta gagctgcaga gcctagatca gtatcagact atcaaaatgc
     1021 aaattcatca tattatgttg aaaatgctaa tgatggttca ggatatcctg taggtacata
     1081 tatcaatgct tctagtaaag gggcgccata taatttacca actacaccat ggaatacatt
     1141 gaaggcctct gactcaaagg aaattgctct tatgacagcg aaacaaactg gagatggcta
     1201 ccaatgggtt attaagttta ataaaggaca tgctccacat caaaatatga ttttctggtt
     1261 tgcattacca gcagaccaag tgccagtagg aagaactgac tttgtaacag ttaattcaga
     1321 tggaacaaat gtacaatgga gtcatggagc aggagcaggt gcaaataaac cacttcaaca
     1381 aatgtgggaa tatggagtaa atgatcctga tcgttcacat gactttaaaa taagaaatag
     1441 aagtggccaa gtaatatata gctggccaac tgtccatgtt tattctttag aagatttatc
     1501 tagagcgagt gattatttta gtgaagctgg agcgacacct gctactaaag catttggtag
     1561 acaaaatttt gaatatatta atggtcaaaa acctgctgaa tcaccgggtg ttcctaaagt
     1621 ttatactttc atcggtcaag gtgatgcaag ttatacaatt tcatttaaaa cacaaggtcc
     1681 aactgttaat aaattgtatt atgcagcagg tgggcgtgct ttagagtaca atcaattatt
     1741 tatgtacagt caactatacg tcgaatcaac gcaagaccat caacaacgtc ttaatggttt
     1801 aagacaagtg gttaatcgta catatcgcat aggtacaact aaacgtgtag aagtgagtca
     1861 aggaaatgta caaacgaaaa aggtattaga aagtacaaac ctaaatatag atgattttgt
     1921 tgatgatcct ttaagttatg ttaagacgcc gagtaataaa gtgttaggtt tttacccaac
     1981 taatgcaaat actaacgctt ttagaccggg gggcgttcaa gaattaaatg aatatcaatt
     2041 aagtcaatta tttactgatc aaaaattaca agaagcagca agaactagaa acccaataag
     2101 attaatgatt ggtttcgact atcctgatgg ttatggtaat agtgaaactt tagttcctgt
     2161 taacttaacg gtattacctg aaatccaaca taatattaaa ttctttaaaa atgacgatac
     2221 tcaaaatatt gctgaaaaac cattttcaaa acaagctggg catccagttt tctatgtata
     2281 tgcaggtaac caagggaatg cttccgtgaa tttaggtggt agcgtaacat ctattcaacc
     2341 attacgtatt aatttaacaa gtaatgagaa ttttacagat aaagattggc aaattacagg
     2401 tattccgcgt acattacaca ttaaaaactc gacaaataga actaataatg ctagagaacg
     2461 taacattgaa cttgttggta atttattacc aggggattac tttggtacga tacgttttgg
     2521 acgtaaagaa caattatttg aaattcgtgt taaaccacat acaccaacaa ttacaacgac
     2581 agctgagcaa ttaagaggta cagcattaca aaaagtgcct gttaatattt cgggaatacc
     2641 gttggatcca tcggcattgg tttatttagt tgcaccaaca aatcaaacta cgaatggtgg
     2701 tagtgaggca gatcaaatac catctggtta tacgatactt gcgactggta cacctgatgg
     2761 ggtgcataat acaattacta tacgaccgca agattatgtt gtattcatac cacctgtagg
     2821 taaacaaatt agagcagtag tttattataa taaagtagtt gcatctaata tgagtaatgc
     2881 tgttactatt ttgccagatg acattccacc aacaatcaat aatcctgttg gaataaatgc
     2941 caaatactat cgaggcgacg aagtcaactt tacaatggga gtctctgata gacattctgg
     3001 tataaaaaat acaactatta ctactttgcc aagtggttgg acatcaaatt taactaaatc
     3061 cgacaacaaa aacggctcat tagctattac aggtagagtc tctatgaatc aggcatttaa
     3121 cagtgatatt acatttaaag tatcagcgac agacaatgtc aataatacga caaatgatag
     3181 tcaatctaaa catgtgtcaa ttcatgtagg taaaattagt gaagatgctc atccgattgt
     3241 attaggaaat actgagaaag ttgtagtagt caatccgact gctgtatcta atgatgaaaa
     3301 gcaaagcata attactgcct ttatgaataa aaaccaaaat ataagaggat atttagcatc
     3361 aactgatcca gtaactgtcg ataataatgg taacgtcaca ttacattacc gtgatggctc
     3421 atcaacaacg cttgatgcta caaatgtgat gacatacgaa ccagttgtga aatctgaata
     3481 tcaaactgcc aatgctgcta aaacagcaac ggtaacgatt gctaaaggac aatcatttaa
     3541 tattggtgat attaaacaat attttacttt aagtaatgga caagctattc caaatggcac
     3601 atttacaaat attacatctg atagaactat tccaactgca caagaagtta gtcaaatgaa
     3661 tgcaggtacg cagttatatc atatagttgc ttcaaatgca tatcataaag acactgaaga
     3721 tttctatatt agtttaaaaa tcgttgatgt gaaacaacct gaaggcgatc aacgtgtcta
     3781 tcgtacgtca acatatgatt taaccactga tgaaatctca aaagtaaaac aagcttttat
     3841 taatgcaaat agagatgtaa ttacgcttgc cgaaggtgat atttcagtta caaatacacc
     3901 taatggtgct aatgtaagta ctattacagt aaatattaat aaaggtcgat taacgaaatc
     3961 attcgcgtct aacctagcta atatgaattt cttgcgttgg gttaatttcc cacaagatta
     4021 tacagtgaca tggacgaatg caaaaattgc aaacagacca acagatggtg gtttatcatg
     4081 gtccgatgac cataaatctt taatttatcg ttatgatgct acattaggca cacaaattac
     4141 aactaatgat attttaacga tgctaaaagc gactactaca gtgcctggat tgcgtaataa
     4201 tattactggt aatgaaaaag cacaagcaga agcaggtgga agaccaaact atagaacaac
     4261 tggttattca caatcaaatg cgacaactga tggtcaacgt caatttacgt tgaatggtca
     4321 agtgattcaa atattagaca tcatcaaccc ttcaaacggt tatggtgggc aacctgttac
     4381 aaattcaaat actcgtgcaa accatagtaa ctcaactgtt gttaacgtaa acgaaccggc
     4441 agctaatggt gctggcgcat ttacaattga ccacgttgta aaaagtaatt ctacacataa
     4501 tgcaagtgat gcagtttata aagcgcagtt atacttaacg ccatatggtc caaaacaata
     4561 tgttgaacat ttaaatcaaa atacaggaaa tactactgac gctattaaca tttattttgt
     4621 accaagtgac ttagtgaatc caacaatttc agtaggtaat tacactaatc atcaagtgtt
     4681 ctcaggtgaa acatttacaa atacgattac agcgaatgat aactttggtg tgcaatcggt
     4741 aactgtacca aatacatcac aaattacagg tactgttgat aataaccatc aacatgtttc
     4801 tgcaacggca ccaaatgtga catcagcaac tagtaagaca atcaatttat tagcaactga
     4861 tacaagtggt aatacagcta caacttcatt caatgtaaca gtgaaacctt tgcgtgataa
     4921 atatcgagtt ggtacttcat caacggctgc taatcctgtt agaattgcca atatttcgaa
     4981 taatgcgaca gtatcacaag ctgatcaaac gacaattatt aattcgttaa cgtttacaag
     5041 taatgcacca aatagaaact atgcaacagc aagcgcaaat gaaatcacta gtaaaacagt
     5101 tagtaatgtc agtcgtactg gaaataatgc caatgtcaca gtaactgtta ctcatcaaga
     5161 tggaacaaca tcaacagtga ctgtacctgt aaagcatgtc attccagaaa tcgttgcaca
     5221 ttcgcattac actgtacaag gccaagactt cccagcaggt aatggttcta gtgcagcaga
     5281 ttactttaag ttatctaatg gtagtgccat tccagatgca acgattacat gggtaagtgg
     5341 acaagcgcca aataaagata atacacgtat tggtgaagat ataacagtaa ctgcacatat
     5401 cttaattgat ggcgaaacaa cgccgattac gaaaacagca acatataaag tagtaagaac
     5461 tgtaccgaaa catgtctttg aaacagccag aggtgtttta tacccaggtg tttcagatat
     5521 gtatgatgcg aaacaatatg ttaagccagt aaataattct tggtcgacaa atgcgcaaca
     5581 tatgaatttt caatttgttg gaacatatgg tcctaacaaa gatgttgtag gtatatcaac
     5641 gcgtcttatt agagtgactt atgataatag acaaactgaa gatttaacta ttttatctaa
     5701 agttaaacct gacccaccaa gaattgacgc aaactctgtg acatataaag caggtcttac
     5761 aaaccaagaa attaaagtta ataacgtatt aaataactcg tcagtaaaat tatttaaagc
     5821 agataataca ccattaaatg tcacaaatat tactcatggt agtggtttta gttcggttgt
     5881 gacagtaagt gacgcgttac caaatggcgg aattaaagca aaatcttcaa tttcaatgaa
     5941 caatgtgacg tatacgacgc aagacgaaca tggtcaagtt gttacagtaa caagaaatga
     6001 atctgttgat tcaaatgata gtgcttctgt tacagtaaca ccacaattac aagcaactac
     6061 tgaaggcgct gtatttatta aaggtggcga cggttttgat ttcggtcatg tagaacgatt
     6121 tattcaaaat ccgccacatg gggcaacggt cgcatggcat gatagtccag atacatggaa
     6181 gaatacagtc ggcaacacac ataaaactgc ggttgtaaca ttacctagtg gtcaaggtac
     6241 gcgtaatgtt gaagttccag tcaaagttta tccagttgct aatgctaagg cgccatcacg
     6301 tgatgtgaaa ggtcaaaatt tgacacatgg tacaaacgct attgattaca ttacatttga
     6361 tccaaatact aatacgaatg gtattacagc agcatgggca aatagacaac aaccaaataa
     6421 ccagcaagca ggcgttcaac atttaaatgt cgatgtcaca tatccaggta tttcagctgc
     6481 taaacgagtt cctgtaactg tgaacgtata tcaatttgaa ttccctcaaa ctacttatac
     6541 aacaacagtt ggtggcactt tagcaagtgg tacgcaagca tcaggatatg cacatatgca
     6601 aaacgcttca ggtttaccaa cagatggatt tacgtataaa tggaatcgtg atactacggg
     6661 tacaaacgat gcaaactggg cagcaatgaa taaaccaaat actgcacaag tcgttaatgc
     6721 aaaatatgat gtcatctata atggacatac atttgcaaca tctttaccag cgaaatttgt
     6781 agtaaaagat gttcaaccag cgaaaccaac tgtcactgaa acagcggcag gagcgattac
     6841 aattgcacct ggtgcgaacc aaacagtcaa tactcatgct ggtaatgtta cgacatatgc
     6901 tgacaaatta gttattaaac gtaatggaaa tgttgtaacg acatttacac gtcgtaataa
     6961 tacgagccca tgggtgaaag aagcatcagc agataatgta acaggtattg ttggaactaa
     7021 taatggtatt actgtggcag caggtacttt caatcctgct gatacaattc aagttgttgc
     7081 aacacaaggt agtggcgaaa caatcagtga cgagcaacgt agtgatgatt tcacagttgt
     7141 cgcaccacaa ccgaaccaag cgactacgaa aatttggcaa aatggtcata ttgatatcac
     7201 gcctaataat ccatcaggac atttaattaa tccaacacaa gcaatggata ttgcttacac
     7261 tgaaaaagtg ggtaatggtg cagaacatag taagacaatt aatgttgttc gtggtcaaaa
     7321 taatcaatgg acaattgcga ataagcctga ctatgtaacg ttagatgcac aaactggtaa
     7381 agtgacgttc aatgccaata ctataaaacc aaattcatca atcacaatta ctccgaaagc
     7441 aggtacaggt cactcagtaa gtagtaatcc aagtacatta actgcaccgg cagctcatac
     7501 tgtcaacaca actgaaattg tgaaagatta tggttcaaat gtaacagcag ctgaaattaa
     7561 caatgcagtt caagttgcta ataaacgtac tgcaacgatt aaaaatggca cagcaatgcc
     7621 tactaattta gctggtggta gcacaacgac gattcctgtg acagtaactt acaatgatgg
     7681 tagtactgaa gaagtacaag agtccatttt cacaaaagcg gataaacgtg agttaatcac
     7741 agctaaaaat catttagatg atccagtaag cactgaaggt aaaaagccag gtacaattac
     7801 gcagtacaat aatgcaatgc ataatgcgca acaacaaatc aataccgcga aaacagaagc
     7861 acaacaagtg attaataatg agcgtgcaac accacaacaa gtttctgacg cactaactaa
     7921 agttcgtgca gcacaaacta agattgatca agctaaagca ttacttcaaa ataaagaaga
     7981 taatagccaa ttagtaacgt ctaaaaataa cttacaaagt tctgtgaacc aagtaccatc
     8041 aactgctggt atgacgcaac aaagtattga taactataat gcgaagaagc gtgaagcaga
     8101 aactgaaata actgcagctc aacgtgttat tgacaatggc gatgcaactg cacaacaaat
     8161 ttcagatgaa aaacatcgtg tcgataacgc attaacagca ttaaaccaag cgaaacatga
     8221 tttaactgca gatacacatg ccttagagca agcagtgcaa caattgaatc gcacaggtac
     8281 aacgactggt aagaagccgg caagtattac tgcttacaat aattcgattc gtgcacttca
     8341 aagtgactta acaagtgcta aaaatagcgc taatgctatc attcagaagc caataagaac
     8401 agtgcaagag gtacaatctg cgttaacaaa tgtaaatcgt gtcaatgagc gattaacgca
     8461 agcaattaat caattagtac ctttagctga taatagtgct ttaagaactg ctaagacgaa
     8521 acttgatgaa gaaatcaata aatcagtaac tactgatggt atgacacaat catcaatcca
     8581 agcatatgaa aatgctaaac gtgcaggtca aacagaaaca acaaatgcac aaaatgttat
     8641 taacaatggt gacgcgacag accaacaaat tgccgcagaa aaaacaaaag tagaagaaaa
     8701 atataatagc ttaaaacaag caattgctgg attaacacca gacttggcac cattacaaac
     8761 tgcaaaaact cagttgcaaa atgatattga tcagccaacg agtacgactg gtatgacaag
     8821 cgcatctgtt gctgcattta atgacaaact ttcagcagct agaactaaaa ttcaagaaat
     8881 tgatcgcgta ctagcatctc atccagatgt agcaacgatt cgtcaaaacg tgacagcagc
     8941 gaatgctgct aaaacagcac ttgatcaagc gcgcaatggc ttaacagtcg ataaagcacc
     9001 tttagaaaat gcgaaaaatc aactacaaca tagtattgat acgcaaacaa gtacaactgg
     9061 tatgacacaa gactctataa atgcatacaa tgcgaagtta acagctgcac gtaataaggt
     9121 tcaacaaatc aatcaagtat tagcaggttc acctactgta gatcaaatta atacaaatac
     9181 gtctgcagca aatcaagcga aatctgattt agatcatgca cgtcaagcgt taacaccaga
     9241 taaagcgccg cttcaaaatg cgaaaacgca attagaacaa agcattaatc aaccaacaga
     9301 tacaacaggt atgacaaccg cttcgttaaa tgcatacaac caaaaattac aagcagcacg
     9361 tcaaaagtta actgaaatta atcaagtgtt gaatggcaac ccaactgtcc aaaatatcaa
     9421 tgataaagtg gcagaggcaa accaagctaa ggatcaatta aatacagcac gtcaaggttt
     9481 aacattagat agacagccag cgttaacaac attacatggt gcatctaact taaaccaagc
     9541 acaacaaaat aatttcacgc aacaaattaa tgctgctcaa aatcatgctg cgcttgaaac
     9601 aattaagtct aacattacgg ctttaaatac tgcgatgacg aaattaaaag acagtgttgc
     9661 ggataataat acaattaaat caggtcaaaa ttacactgac gcaacaccag ctaataaaca
     9721 agcctatgat aatgcagtta atgcggctaa aggtgtcatt ggagaaacga ctaatccaac
     9781 gatggatgtt aacacagtga accaaaaagc agcatctgtt aaatcgacga aagatgcttt
     9841 agatggtcaa caaaacttac aacgtgcgaa aacagaagca acaaatgcga ttacgcatgc
     9901 aagtgattta aaccaagcac aaaagaatgc attaacacaa caagtgaata gtgcacaaaa
     9961 cgtgcaagca gtaaatgata ttaaacaaac gactcaaagc ttaaatactg ctatgacagg
    10021 tttaaaacgt ggcgttgcta atcataacca agtcgtacaa agtgataatt atgtcaacgc
    10081 agatactaat aagaaaaatg attacaacaa tgcatacaac catgcgaatg acattattaa
    10141 tggtaatgca caacatccag ttataacacc aagtgatgtt aacaatgctt tatcaaatgt
    10201 cacaagtaaa gaacatgcat tgaatggtga agctaagtta aatgctgcga aacaagaagc
    10261 gaatactgca ttaggtcatt taaacaattt aaataatgta caacgtcaaa acttacaatc
    10321 gcaaattaat ggtgcgcatc aaattgatgc agttaataca attaagcaaa atgcaacaaa
    10381 cttgaatagt gcaatgggta acttaagaca agctgttgca gataaagatc aagtgaaacg
    10441 tacagaagat tatgcggatg cagatacagc taaacaaaat gcatataaca gtgcagtttc
    10501 aagtgctgaa acaattatta atcaaacagc taatccgaca atgtctgttg atgatgttaa
    10561 tcgtgcaact tcagctgtta ctactaataa aaatgcatta aatggtgatg aaaaattagt
    10621 acaatctaaa acagatgctg caagagcaat tgatgcatta ccacatttaa ataatgcaca
    10681 aaaagcagat gttaaatcta aaattaatgc tgcatcaaat attgctggtg taaataccgt
    10741 taaacaacaa ggtacagatt taaatacagc gatgggtaac ttgcagggtg caatcaatga
    10801 tgaacaaacg acgcttaata gtcaaaatta tcaagatgcg acacctagta agaaaacagc
    10861 atacacaaat gcggtgcaag ctgcgaaaga tattttaaat aaatcaaatg gtcaaaataa
    10921 aacgaaagat caagttactg aagcgatgaa tcaagtgaat tcggctaaaa ataacttaga
    10981 tggtacgcgt ttattagatc aagcgaagca aacagcgaaa cagcagttaa ataatatgac
    11041 gcatttaaca actgcacaaa aaacgaattt aacaaatcaa attaatagtg gtactactgt
    11101 tgctggtgtt catacggttc aatcaaatgc caacacatta gatcaagcga tgaatacgtt
    11161 aagacaaagt attgctaaca atgatgcgac taaagcaagt gaagattacg tagatgctaa
    11221 taatgataag caaacagcat ataacaacgc ggtagctgct gctgaaacga ttattaatgc
    11281 gaatagtaat ccagaaatga atccaagtac gattacacaa aaagcagagc aagtgaatag
    11341 ttctaaaacg gcacttaacg gtgatgaaaa cttagctacg gcaaaacaaa atgcgaaaac
    11401 gtacttaaac acattaacga gtattacaga tgctcaaaag aacaatttga ttagtcaaat
    11461 tagtagtgcg acaagagtga gtggtgttga tactgtaaaa caaaatgcac aacatttaga
    11521 tcaagctatg gctaacttac aaaatggtat taacaacgaa tctcaagtga aatcatctga
    11581 gaaatatcgt gatgctgata caaataaaca acaagagtat gataatgcta ttactgcagc
    11641 gaaagcgatt ttaaataaat cgacaggtcc aaacactgcg caaaatgcag ttgaagcagc
    11701 attgcaacgt gttaatactg cgaaagatgc attgaatggt gatgcaaaat taattgcagc
    11761 tcaaaacgca gcgaaacaac atttaggtac tttaacgcat atcactacag cacaacgcaa
    11821 tgatttaaca aatcaaattt cacaagctac aaacttagct ggtgttgaat cagttaaaca
    11881 aagtgcaaat agtttagatg gcgcaatggg taacttacaa acggctatca acgataagtc
    11941 aggaacatta gcgagccaaa acttcttgga tgctgatgag caaaaacgta atgcttacaa
    12001 tcaagctata tcagctgccg aaaccatttt aaataaacaa actggaccga atacagcgaa
    12061 aacagcggtt gaacaagcac ttaataatgt taatagtgcg aaacatgcat taaatggtac
    12121 gcaaaactta aataatgcga aacaagcagc gattacagca attaatggcg catctgattt
    12181 aaatcaaaaa caaaaagatg cattaaaagc acaagctaat ggtgctcaac gcgtatctaa
    12241 tgcaaatgat gtacaacgta atgcgactga actgaacacg gcaatgggtc aattacaaca
    12301 tgccatcgca gataagacga atacgttagc aagcagtaaa tatgtcaacg ccgatagcac
    12361 taaacaaaat gcttacacaa ctaaagttac caatgctgaa catattatta gcggtacgcc
    12421 aacggttgtt acaacacctt cagaagtaac agctgcagct aatcaagtaa acagcgcgaa
    12481 acaagaatta aatggtgacg aaagattacg tgttgcaaaa caaaacgcca atactgctat
    12541 tgatgcatta acgcaattaa atactcctca aaaagctaaa ttaaaagaac aagtgggaca
    12601 agccaataga ttagaagacg tacaatctgt tcaaacaaat ggacaatcat tgaacaatgc
    12661 aatgaaaggc ttaagagata gtattgctaa cgaaacaaca gtcaaagcaa gtcaaaacta
    12721 tacagacgca agtccgaata accaatcaac atataatagc gctgtgtcaa atgcgaaagg
    12781 tatcattaat caaactaaca atccaactat ggatactagt gcgattaccc aagctacaac
    12841 acaagtgaat aatgctaaaa atggtttaaa cggtgctgaa aacttaagaa atgcacaaaa
    12901 cactgctaag caaaacttaa atacgttatc acacttaaca aataaccaaa aatctgcaat
    12961 ctcatcacaa attgatcgtg caggtcatgt gagtgaggta acagctgcta aaaatgcagc
    13021 aactgagtta aacgcgcaaa tgggcaactt ggaacaagct atccatgatc aaaacacagt
    13081 taaacaaggt gttaacttca ctgatgcaga taaagctaaa cgtgatgctt atacaaatgc
    13141 ggtaagcaga gcagaaacaa ttctgaataa aacgcaaggt gcaaatacgt ctaaacaaga
    13201 tgttgaagcg gctattcaaa atgttacaag tgctaaaaat gcattgaatg gtgatcaaaa
    13261 cgttacaaat gcgaagaatg cagctaaaaa tgcattaaat aacttaacgt caattaataa
    13321 tgcacaaaaa cgtgacttaa caactaaaat tgatcaagca acaacagtag ctggtgttga
    13381 agcggtatct aatacaggta cacaattgaa tacagcgatg gctaacttgc aaaatggtat
    13441 taatgataaa gcgaatactt tagcgagcga aaactatcat gatgctgatt cagataagaa
    13501 aactgcttat actcaagccg ttacgaacgc agaaaatatt ttaaataaaa atagtggatc
    13561 aaatttagat aaagctgccg ttgaaaacgc gttgtcacaa gtgacaaatg cgaaaggtgc
    13621 cctaaatggt aaccataatt tagagcaagc taaatcaaat gcaaacacta ctataaacgg
    13681 ccttcaacat ttaacaacag cacaaaaaga taaattgaaa caacaagtgc aacaagcaca
    13741 aaatgttgca ggtgtagata ctgttaaatc aagtgccaac acattaaatg gtgctatggg
    13801 tacgttaaga aatagcatac aagataacac agctacgaaa aatggccaaa actatcttga
    13861 tgctacagaa cgtaacaaaa caaactataa caatgctgtt gatagtgcta atggtgtcat
    13921 taatgcaaca agcaatccaa atatggatgc taatgcaatt aaccaaatcg ctacacaagt
    13981 gacatcaacg aaaaatgcat tagatggtac acataattta acgcaagcga aacaaacagc
    14041 aacaaatgcc atcgatggtg ctactaactt aaataaagcg caaaaagatg cgttaaaagc
    14101 acaagttaca agtgcgcaac gtgttgcaaa tgtaacaagt atccaacaaa ctgcaaatga
    14161 acttaataca gctatgggtc aattacaaca tggtattgat gatgaaaatg caacaaaaca
    14221 aactcaaaaa tatcgtgacg ctgaacaaag taagaaaact gcttatgatc aagctgtagc
    14281 tgctgcgaaa gcaattttaa ataaacaaac aggttccaat tcagataaag cagcagttga
    14341 ccgtgcatta caacaagtaa caagtacgaa agatgcattg aatggggatg ctaaactggc
    14401 agaagcgaaa gcggcagcta gacaaaactt aggtacttta aaccatatta cgaatgcaca
    14461 acgtactgcg ttagaaggtc aaatcaatca agcgacgact gttgatggcg ttaatactgt
    14521 aaaaacaaat gccaatacat tagacggcgc tatgaatagc ttacaaggtg caatcaatga
    14581 taaagatgcg acattaagaa atcaaaatta tcttgatgca gatgaatcaa aacgaaatgc
    14641 atatacgcaa gctgtcacag cggctgaagg cattttaaat aaacaaacag gtggtaacac
    14701 atctaaagca gacgttgata atgcattaaa tgcagttaca agagcgaaag cggctttaaa
    14761 tggtgctgaa aacttaagaa atgcgaaaac ttcagcaaca aatacgatta atggtttacc
    14821 taacttaaca caattacaaa aagacaactt gaagcatcaa gttgaacaag cgcaaaatgt
    14881 agttggtgta aatggtgtta aagataaagg taatacatta aatactgcca tgggtgcatt
    14941 acgtacaagt atccaaaatg ataatacgac gaaaacaagt caaaattatc ttgatgcatc
    15001 tgatagcaac aaaaataatt acaatactgc tgtaaataat gcaaatggtg ttattaatgc
    15061 aacgaacaat ccaaatatgg atgctaatgc gattaatgac atggcaaatc aagtcaatac
    15121 aacaaaagca gcgttaaatg gtgcacaaaa cttagctcaa gctaaaacaa atgcgacgaa
    15181 cacaattaac aacgcgcaag acttaaacca aaaacaaaaa gatgcattaa aaacacaagt
    15241 taacaatgca caacgtgtat ctgatgcaaa taacgttcaa catacagcta ctgaattgaa
    15301 cggtgcgatg acagcactta aagcagctat tgcggataaa gaaagaacaa aagcaagcgg
    15361 taattatgtc aatgctgatc aagaaaaacg tcaagcgtat gattcaaaag tgactaacgc
    15421 tgaaaatatc attaatggta caccaaatgc gacattaaca gtcaatgacg taaatagtgc
    15481 ggcatcacaa gtcaatgcgg ctaaaacagc attaaatggt gataacaact tacgtgtagc
    15541 gaaagagcat gctaacaata caattgacgg cttagcacaa ttgaataatg tacaaaaagc
    15601 aaaattaaaa gaacaagttc aaagtgcaac tacattagat ggtgttcaaa ctgttaaaaa
    15661 tagttctcaa acgttgaata cagcgatgaa aggcttaaga gatagtattg cgaatgaagc
    15721 aacgattaaa gcaggtcaaa actacactga cgcaagtcca aataatcgta acgagtacga
    15781 cagcgcagtt actgcagcaa aagcaatcat taatcaaaca tcgaacccaa cgatggaacc
    15841 aaatactatt acgcaagcaa catcacaagt gacaactaaa gaacatgcat taaatggtgc
    15901 gcaaaactta gctcaagcta agacaacagc gaaaaacaac ttgaataact taacatcaat
    15961 taacaatgca caaaaagatg cgttaacgcg taacattgat ggtgcaacta cagtagctgg
    16021 tgtaaatcaa gaaactgcaa aagcaacaga attaaataac gcaatgcaca gtttacaaaa
    16081 tggtatcaat gatgagacac aaacaaaaca aactcagaaa tacctagatg ctgagccaag
    16141 taagaaatca gcttatgatc aagcagtaaa tgcagcaaaa gcaattttaa caaaagctag
    16201 tggtcaaaat gtagacaaag cagcagttga acaagcatta caaaatgtga acagtacgaa
    16261 gacggcgttg aacggtgatg cgaaattaaa tgaagctaaa gctgctgcga aacaaacgtt
    16321 aggtacatta acacacatta ataatgcaca acgtaatgcg ttagataatg aaattacaca
    16381 agcaacaaat gttgaaggtg ttaatacagt taaagccaaa gcgcaacaat tagatggtgc
    16441 tatgggtcaa ttagaaacat caattcgtga taaagacacg acgttacaaa gtcaaaatta
    16501 tcaagatgct gatgatgcta aacgaacggc ttattctcaa gcagtaaatg cagcagcaac
    16561 tattttaaat aaaacagctg gaggaaatac acctaaagca gatgtcgaaa gagcaatgca
    16621 agctgttaca caagccaata ctgcattaaa cggtattcaa aacttagaac gtgcgaaaca
    16681 ggctgcgaac acagcgatta caaatgcttc ggacttaaat acaaaacaaa aagaagcatt
    16741 gaaagcacaa gtaacaagtg caggacgcgt atctgcagca aatggtgttg aacatactgc
    16801 gactgaatta aatactgcga tgacagcttt aaaacgtgcc attgctgata aagctgacac
    16861 aaaagctagt ggtaattatg tcaatgctga tgcgaataaa cgccaagcat atgatgaaaa
    16921 agtgacagct gcagaacata tcgttagtgg tacaccaaca ccaacgttaa caccatcaga
    16981 tgttacaaat gcagcaacgc aagtaacgaa tgcgaagacg cagttaaacg gcaatcataa
    17041 tttagaagta gcgaaacaaa atgctaacac agcaattgat ggtttaactt ctttaaatgg
    17101 tccgcaaaaa gcaaaactta aagaacaagt gggtcaagcg acgacgttgc caaatgttca
    17161 aactgttcgt gataatgcac aaacattaaa cactgcaatg aaaggtctac gagatagcat
    17221 tgcgaatgaa gcaacgatta aagcaggtca aaactacaca gatgcaagtc aaaacaaaca
    17281 aaatgactac aacaatgcag tcactgcagc aaaagcaatc attggtcaaa caactagtcc
    17341 atcaatgatt gcgcaagaaa ttaatcaagc gaaagaccaa gtgacagcta aacaacaagc
    17401 gttaaacggt caagaaaact taagaactgc gcaaacaaat gcgaagcaac atttgaatgg
    17461 cttaagtgac ttaactaatg cacaaaaaga tgcagcgaaa cgccaaatcg aaggtgcaac
    17521 gcatgttaat gaagtaacac aagcgcaaaa taatgcggac gcattaaata cagctatgac
    17581 gaacttgaaa aatggtattc aagatcaaaa tacgattaag caaggtgtta acttcactga
    17641 tgcagatgaa gcgaaacgta atgcatatac aaatgcagtg acgcaagctg aacaaatttt
    17701 aaataaagca caaggtccaa atactgcaaa agacggtgtc gaaactgcgt tacaaaatgt
    17761 acaacgtgct aaaaacgaat tgaacggtaa tcaaaatgtt gcgaacgcta agacaactgc
    17821 gaaaaatgca ttgaataacc ttacatcaat taataatgca caaaaagcag cattgaaatc
    17881 acaaattgaa ggtgcgacaa cagttgcagg tgtaaatcaa gtgtctacaa tggcatctga
    17941 attaaatact gcaatgagca acttacaacg tggtattaat gacgaagcag ctacaaaagc
    18001 agctcagaaa tatactgaag cagatagaga taaacaaact gcatacaatg atgctgtaac
    18061 agcagctaaa acgttattag ataaaacagc tggttcaaat gacaataaag tagccgttga
    18121 acaagcatta caacgtgtga atactgctaa aacagcatta aatggtgacg cgcgattaaa
    18181 tgaagcgaag aacacagcta aacaacaatt agcgacaatg tcacatttaa ctaatgctca
    18241 aaaagcaaac ttaacagaac aaattgaacg tggtacaact gttgctggtg ttcaaggcat
    18301 ccaagcaaat gctggtactt taaatcaagc aatgaatcaa ttaagacaaa gtattgcttc
    18361 taaagatgcg actaaatcaa gcgaagatta tcaagacgcg aatgcagatt tacaaaatgc
    18421 atacaatgat gcggtaacta atgctgaagg tattattagt gcaacgaata accctgaaat
    18481 gaatcctgat acaattaacc aaaaagcgag ccaagtgaac agtgcgaagt ctgcattgaa
    18541 cggtgatgaa aaattagcag cagcaaaaca aactgcgaaa tcagatatcg gtcgtttgac
    18601 agacttgaac aatgcacaac gaactgcggc aaatgctgaa gtggatcaag caccaaatct
    18661 tgcagctgtc acagcggcta aaaataaagc aacatcgtta aacacagcga tgggtaattt
    18721 gaaacatgca cttgctgaaa aggataatac gaaacgtagt gtcaattaca cagatgcgga
    18781 tcaaccaaaa caacaagcgt atgatactgc agttacacaa gcagaagcaa ttactaatgc
    18841 aaatggcagt aacgcgaatg aaacacaagt tcaagcagcg cttaaccaat tgaatcaagc
    18901 taaaaacgac ttgaatggtg ataataaagt tgctcaagcg aaagaaacag caaaacgtgc
    18961 attagcttca tatagtaact tgaataacgc gcaatcaact gcagcaacta gtcaaattga
    19021 caatgcaacg acagtagcag acgtaactgc tgcacaaaat actgctaatg aattaaatac
    19081 agcaatgggt caacttcaaa atggtattaa tgaccaaaac actgttaaac aacaagtgaa
    19141 ctttacagat gctgaccaag gtaagaaaga tgcttacaca aatgctgtta cgaatgctca
    19201 aggtatttta gataaagcaa acggtcaaaa tatgacaaaa gcacaagttg aagctgcatt
    19261 aaatcaagta acgactgcta agaatgcttt aaacggtgat gcaaatgtaa gacaagcaaa
    19321 atcagatgcg aaagcaaact taggtacatt aacacactta aataatgcac aaaaacaaga
    19381 tttaacatca caaatcgaag gtgcaacaac agtcaacggt gtaaatagtg ttaaaacgaa
    19441 agcacaagac ttagatggtg caatgcaacg attagagtca gcaatcgcaa ataaagatca
    19501 aactaaagcg agcgaaaact acattgacgc agatccaact aagaaaacag catttgataa
    19561 tgccatcaca caagctgaat cttacttaaa taaagatcat ggtacgaata aagataagca
    19621 agctgttgaa caagcaattc aaagtgtaac gtctactgaa aatgctttga acggtgacgc
    19681 gaacttacaa tgcgctaaaa ctgaagctac acaagctatc gataacttga cacaattgaa
    19741 tacaccgcaa aaaacagcat tgaaacaaca agtgaatgct gcacaacgcg tatcaggtgt
    19801 aactgatctg aaaaatagtg ctacatcact taataatgcg atggatcaat taaaacaagc
    19861 aattggtgat catgacacaa ttgtagctgg tggtaattac actaacgcaa gtcctgataa
    19921 acaaggtgct tacactgatg catataatgc tgcgaagaat atcgtaaatg gttcacctaa
    19981 tgtgattaca aatgcagcag atgttactgc ggcaacacaa cgtgtcaata atgctgaaac
    20041 aagtttaaat ggtgatacaa acttagcaac tgcgaagcaa caagctaaag atgcattacg
    20101 tcaaatgaca catttatctg atgcacaaaa acaaagtatt actggtcaaa ttgatagcgc
    20161 gacacaagta actggtgtac aaagtgtgaa agacaatgca acaaatcttg acaatgcaat
    20221 gaatcaactt cgaaatagta ttgcgaataa agatgaagta aaagcgagtc aaccatatgt
    20281 tgatgcagat acagataaac aaaatgcata caatacagca gttacaagtg ctgaaaatat
    20341 cattaatgca acgagtcagc caacacttga tccatctgca gtaacacaag cagctaatca
    20401 agtgaacact aacaaaactg cgcttaatgg tgcgcaaaac ttagcaaata aaaagcaaga
    20461 aacaactgct aacatcaacc gattaagtca tttaaacaat gctcaaaagc aagatttaaa
    20521 tacacaagtg acaaatgcac caaatattag cacagtaaat caagtgaaaa ctaaagctga
    20581 acaattagat caagcaatgg aacgtttaat caacggaatc caagacaaag atcaagtgaa
    20641 acaaagtgtt aactttacag atgcagatcc agaaaaacaa acagcataca acaatgcggt
    20701 aactgctgct gaaaatatta ttaatcaagc aaatggtaca aatgcgaacc aatcacaagt
    20761 tgaagcagca ctttcaactg taacaactac taaacaagcg ttgaatggtg atagaaaagt
    20821 aacagatgct aaaaacaatg caaaccaaac attatctacg ttagataact taaacaatgc
    20881 acaaaaaggt gctgttactg gaaacatcaa tcaagcgcac actgtagctg aagtaacgca
    20941 agccattcaa accgctcagg aactgaatac agcgatgggt aacttgaaaa atagcttgaa
    21001 tgataaagac actacacttg gcagtcaaaa ctttgcagat gcagatccag agaagaaaaa
    21061 tgcatacaat gaagcggttc gtaatgctga aaatatttta aataaatcta caggtacgaa
    21121 cgtgcctaaa gatcaagttg aagcagctat gaatcaagtg aatactacaa aagcagcgct
    21181 taatggtact caaaaccttg aaaaagcgaa acaacacgca aatacagcaa ttgacggttt
    21241 aagccattta acaaatgcac aaaaagaggc attaaaacaa ttggtacaac aatcgactac
    21301 tgttgcagaa gcacaaggta atgaacaaaa agcaaacaat gttgatgcag caatggacaa
    21361 attacgtcaa agtattgcag ataatgcgac aacaaaacaa aaccaaaatt atactgatgc
    21421 aagtccgaat aaaaaggatg cgtacaataa tgctgtcaca actgcacaag gtattattga
    21481 tcaaactaca aacccttcat tagatccgac tgttatcaat caagctgctg gacaagtaag
    21541 cacgtctaaa aatgctttaa atggtaatga aaacttagag gcagtgaagc aacaagcaac
    21601 gcaatcttta ggttcattag acaacttaaa taatgcgcaa aaacaagctg ttactaatca
    21661 aattaatggc gcgcatactg ttgatgaagc aaatcaaatt aagcaaaatg cgcaaaactt
    21721 aaatactgcg atgggtaact tgaaacaagc gatagctgat aaagatgcta cgaaagcaac
    21781 agttaacttc actgatgcag atcaagcaaa acaacaagca tataacactg cagttacaaa
    21841 tgctgaaaat atcatttcaa aagctaatgg tggtaatgca acacaaactg aagttgaaca
    21901 agcaatccaa caagtaaatg cagcaaaaca agcattaaat ggtaatgcca acgttcaaca
    21961 tgcaaaagac gaagcaacag cattaattaa taactctaat gatcttaacc aagcacagaa
    22021 agatgcatta aaacaacaag tacaaaatgc aactactgta gctggtgtaa acaatgttaa
    22081 acaaacggcg caagagttaa acaatgcgat gacacaatta aaacaaggcg ttgcagataa
    22141 agaacaaaca aaagctgatg gtaactttgt caatgcagat tctgacaagc aaaatgcata
    22201 taatcaagca gtagcgaaag ctgaagcatt aattagtggt acgcctgatg ttgtcgttac
    22261 acctagcgaa attactgcag cgttaaataa agttacgcaa gctaaaaatg atttaaatgg
    22321 taatacaaac ttagcaacgg cgaaacaaaa tgttcaacat gctattgatc aattgccaaa
    22381 cttaaaccaa gcgcaacgtg atgaatacag caaacaaatc acgcaagcaa cacttgtacc
    22441 aaacgtcaat gctattcaac aagcggcaac aacgcttaat gacgcgatga cacaattgaa
    22501 acaaggtatt gcgaataaag cacaaattaa aggtagcgag aactatcacg atgctgatac
    22561 tgacaagcaa acagcatatg ataatgcagt aacaaaagca gaagaattgt taaaacaaac
    22621 aacaaatcca acaatggatc caaatacaat tcaacaagca ttaactaaag tgaatgacac
    22681 aaatcaagca cttaacggta atcaaaaatt agctgatgcc aaacaagatg ctaagacaac
    22741 acttggtaca ctagatcatt taaatgatgc tcaaaaacaa gcgctaacaa ctcaagttga
    22801 acaagcacca gatattgcaa cagttaataa tgttaagcaa aatgctcaaa atctgaataa
    22861 tgctatgact aacttaaaca atgcattaca agataaaact gagacattaa atagcattaa
    22921 ctttactgat gcagatcaag ctaagaaaga tgattatact aatgcggttt cacatgcaga
    22981 aggtatttta tctaaagcaa atggcagcaa tgcaagtcaa actgaagtgg aacaagcgat
    23041 gcaacgtgtg aacgaagcga aacaagcatt gaatggtaat gacaatgtac aacgtgcaaa
    23101 agatgcagcg aaacaagtaa ttacaaatgc aaatgattta aatcaagcgc aaaaagatgc
    23161 attaaaacaa caagtcgatg ctgcgcaaac tgttgcaaat gtaaacacga ttaagcaaac
    23221 agcacaagat ttaaatcaag caatgacaca attgaaacaa ggtattgcag ataaagacca
    23281 aactaaagca aatggtaact ttgtcaatgc tgatactgat aagcaaaatg catataacaa
    23341 tgcggtagcg catgctgaac aaatcattag tggtacacca aatgcaaacg tggatccaca
    23401 acaagtggct caagcgttac aacaagtgaa tcaagctaag ggtgatttaa acggtaacca
    23461 taacttacaa gttgctaaag acaatgcaaa tacagccatt gatcagttac caaacttaaa
    23521 tcaaccacaa aaaacagcat taaaagacca agtgtcgcat gcagaacttg ttacaggtgt
    23581 taatgctatt aagcaaaatg ctgatgcgtt aaataatgca atgggtacgt tgaaacaaca
    23641 aattcaagcg aatagtcaag taccacaatc agttgacttt acacaagcgg atcaagacaa
    23701 acaacaagct tataacaatg cagctaacca agcgcaacaa atcgcaaatg gcacaccaac
    23761 acctgtattg gcgcctgata cagtaacaaa agcagttaca actatgaatc aagcgaaaga
    23821 tgcattaaac ggtgatgaaa aattagcgca agcgaaacaa gatgctttag caaatcttga
    23881 tacgttacgt gacttaaatc aaccacaacg tgatgcatta cgaaaccaaa tcaatcaagc
    23941 acaagcttta gctacagttg aacaaactaa acaaaatgca caaaatgtga atacagcaat
    24001 gggtaacttg aaacaaggta ttgcaaataa agatactgtg aaagcaagtg agaactacca
    24061 cgatgctgat gtcgataagc aaacagcata tacaaatgca gtgtctcaag cggaaggtat
    24121 tatcaatcaa acgacaaatc caacgcttaa cccagatgac attactcgtg cattaactca
    24181 agtgactgat gctaaaaata gcttaaacgg tgaagctaaa ttagccactg aaaagcaaaa
    24241 tgctaaagat gccgtaagtg gaatgacgca tttaaacgat gctcaaaaac aagcattaaa
    24301 aggtcaaatc gatcaatcgc ctgaaattgc tacagtgaac caagttaaac aaacagcaac
    24361 gagcctagat caagcaatgg atcaattatc acaagctatt aatgataaag atcaaatatt
    24421 agcggacggt aattacttaa atgcagatcc tgacaaacaa aatgcgtata aacaggcagt
    24481 agcaaaagct gaagcattat tgaataaaca aagtggtact aatgaagtac aagcacaagt
    24541 tgaaagcatc actaatgaag tgaacgcagc gaaacaagca ttaaatggta atgacaattt
    24601 ggcaaatgca aaacaacaag caaaacaaca attggcgaac ttaacacact taaatgatgc
    24661 acaaaaacaa tcatttgaaa gtcaaattac acaagcgcca cttgttacag atgtcactac
    24721 gattaatcaa aaagcacaaa cgttagatca tgcgatggaa ttattaagaa atagtgttgc
    24781 ggataatcaa acgacattag cgtctgaaga ttatcatgat gcaactgcgc aaagacaaaa
    24841 tgactataac aaagctgtaa cagctgctaa taatatcatt aatcaaacta catcgcctac
    24901 gatgaatcca gatgatgtta atggtgcaac gacacaagtg aataatacga aagttgcatt
    24961 agatggtgat gaaaaccttg cagcagctaa acaacaagca aacaacagac ttgatcaatt
    25021 agatcatttg aataatgcgc aaaagcaaca gttacaatca caaattacgc aatcatctga
    25081 tattgctgca gttaatggtc acaaacaaac agcagaatct ttaaatactg cgatgggtaa
    25141 cttaattaat gcgattgcag atcatcaagc cgttgaacaa cgtggtaact tcatcaatgc
    25201 tgatactgat aaacaaactg cttataatac agcggtaaat gaagcagcag caatgattaa
    25261 caaacaaact ggtcaaaatg cgaaccaaac agaagtagaa caagctatta ctaaagttca
    25321 aacaacactt caagcgttaa atggagatca taatttacaa gttgctaaaa caaatgcgac
    25381 gcaagcaatt gatgctttaa caagcttaaa tgatcctcaa aaaacagcat taaaagacca
    25441 agttacagct gcaactttag taactgcagt tcatcaaatt gaacaaaatg cgaatacgct
    25501 taaccaagca atgcatggtt taagacagag cattcaagat aacgcagcaa ctaaagcaaa
    25561 tagcaaatat atcaacgaag atcaaccaga gcaacaaaac tatgatcaag ctgttcaagc
    25621 cgcaaataat attatcaatg aacaaactgc aacattagat aataatgcga ttaatcaagt
    25681 agcggcaact gtgaatacaa cgaaagcagc attacatggt gatgtgaaat tacaaaatga
    25741 taaagatcat gctaaacaaa cggttagcca attagcacat ctaaacaatg cacaaaaaca
    25801 tatggaagat acgttaattg atagtgaaac aactagaaca gcagttaagc aagatttgac
    25861 tgaagtacaa gcattagatc aacttatgga tgcattacaa caaagtattg ctgacaaaga
    25921 tgcaacacgt gcgagcagtg catatgtcaa tgcagaaccg aataaaaaac aagcctatga
    25981 tgaagcagtt caaaatgctg agtctatcat tgcaggatta aataatccaa ctatcaataa
    26041 aggtaatgta tcaagtgcga ctcaagcagt aatatcatct aaaaatgcat tagatggtgt
    26101 tgaacgatta gctcaagata agcaaactgc tggaaattct ctaaatcatt tagatcaatt
    26161 aacaccagct caacaacaag cgctagaaaa tcaaattaat aatgcaacaa ctcgtgataa
    26221 agtggctgaa atcattgcac aagcgcaagc attaaatgaa gcgatgaaag cattaaaaga
    26281 aagtattaag gatcaaccac aaactgaagc aagtagtaaa tttattaacg aggatcaagc
    26341 gcaaaaagat gcatatacgc aagcagtaca acacgcgaaa gatttgatta acaaaacaac
    26401 tgatcctaca ttagctaaat caatcattga tcaagcgaca caggcagtga ctgatgttaa
    26461 aaacaattta catggtgatc aaaaactagc tcaagataag caacgtgcaa cagaaacgtt
    26521 aaataacttg tctaacttga atacaccaca acgtcaagca cttgaaaatc aaatcaataa
    26581 tgcagcaact cgtggtgaag tagcacaaaa attaactgaa gcacaagcac ttaaccaagc
    26641 aatggaagct ttacgtaata gcattcaaga tcaacaacaa acagaatctg gtagcaagtt
    26701 tattaatgaa gataaaccgc aaaaagatgc ttaccaagca gcagttcaaa atgcaaaaga
    26761 tttaattaac caaacaggta atccaacgct tgataaagca caagttgaac aattgacaca
    26821 tgcttttaaa caagctaaag ataacctaca cggtgatcaa aaacttgcag acgataaaca
    26881 acatgcggtt actgatttaa atcaattaaa tggtttgaat aatccgcaac gtcaagcact
    26941 tgaaagccaa ataaacaacg cagcaactcg tggcgaagta gcgcaaaaat tagctgaagc
    27001 aaaagcgctt gatcaagcaa tgcaagcatt acgaaatagt attcaagatc aacaacaaac
    27061 ggaagcgggt agcaagttta tcaatgaaga taaaccgcaa aaagatgctt accaagcagc
    27121 agttcaaaat gcaaaagatt taattaacca aacaggtaat ccaacactcg acaaatcaca
    27181 agtagaacaa ttaacacaag cagtaacaac tgcaaaagat aatctacatg gtgatcaaaa
    27241 acttgctcgt gatcaacaac aagcagtaac aactgtaaat gcattgccaa acttaaatca
    27301 tgcacaacaa caaacattaa ctgatgctat aaatgcagcg cctacaagaa cagaggttgc
    27361 acaacatgtt caaactgcta ctgaacttga tcacgcgatg gaaacattga aaaataaagt
    27421 tgatcaagtg aatacagata aggctcaacc aaattacact gaagcgtcaa ctgataaaaa
    27481 agaagcagta gatcaagcgt tacaagctgc acaaagcatt acagatccaa ctaatggttc
    27541 aaatgcgaat aaagacgctg tagaacaagc attaactaag cttcaagaaa aagtgaatga
    27601 gttaaatggt aatgagagag tcgctgaagc taaaacacaa gcgaaacaaa ctattgacca
    27661 attaacacat ttaaatgctg atcaaattgc aactgctaaa caaaatattg atcaagcgac
    27721 gaaacttcaa ccaatcgctg aattagtaga tcaagcaacg caattgaacc aatcaatgga
    27781 tcaattacaa caagcagtta atgaacatgc taacgttgag caaactatag attacacaca
    27841 agcagattca gataagcaaa aggcttataa acaagcgatt gctgatgctg aaaatgtatt
    27901 gaaacaaaat gcgaataagc aacaagtgga tcaagcactt caaaaaattt taaatgcaaa
    27961 acaagcatta aatggtgatg aacgtgtagc acttgctaaa acaaatggta aacatgacat
    28021 cgaccaattg aatgcattaa acaatgctca acaagatgga tttaaaggtc gcatcgatca
    28081 atcaaacgat ttaaatcaaa tccaacaaat tgtagatgag gctaaggtac ttaatcgtgc
    28141 aatggatcaa ttgtcacaag aaatcactgg caatgaagga cgcacgaaag gtagcacgaa
    28201 ctatgtcaat gcagatacac aagtcaaaca agtatatgat gaagcggttg ataaagcgaa
    28261 acaagcactt gataaatcgt ctgggcaaaa cttaactgca gaacaagtta tcaaattaaa
    28321 tgatgcagtc actgcagcta agaaagcatt aaatggtgaa gaaagactta ataatcgtaa
    28381 agctgaagca ttacaaagat tggatcaatt aacacatcta aacaatgctc aaagacaatt
    28441 agcaatccaa caaattaata atgctgaaac gctaaataaa gcatctcgag caattaatag
    28501 agcaactaaa ttagataatg caatgggtgc agtacaacaa tatattgacg aacagcacct
    28561 tggtgttatc agcagcacaa attacatcaa tgcagatgac aatttgaaag caaattatga
    28621 taatgcaatt gcgaatgcag cacatgagtt agataaagtg caaggtaatg caattgcaaa
    28681 agctgaagca gagcaattga aacaaaatat tatcgatgct caaaatgcat taaatggaga
    28741 ccaaaacctt gcaaatgcca aagataaagc aaatgcgttt gttaattcgt taaatggatt
    28801 aaatcaacag caacaagatc ttgcacataa agcaattaac aatgccgata ctgtatcaga
    28861 tgtaacagat attgttaata atcaaattga cttaaatgat gcaatggaaa cattgaaaca
    28921 tttagttgac aatgaaattc caaatgcaga gcaaactgtc aattaccaaa acgctgacga
    28981 taatgctaaa acaaacttcg atgatgccaa acgtctagca aatacattgc taaatagtga
    29041 taacacaaat gtgaatgata tcaatggcgc aatccaagca gtcaatgatg caatccataa
    29101 tcttaatggt gatcaacgac tacaagatgc taaagacaag gcaattcaat caattaatca
    29161 agctttagct aataagctaa aagaaatcga agcttcaaat gcgacggatc aagacaagct
    29221 tattgcgaaa aataaagcag aagaattggc aaacagcatc atcaacaaca ttaataaagc
    29281 aacaagtaat caggctgtat ctcaagttca aacagcaggc aaccacgcga ttgaacaagt
    29341 gcatgctaat gaaataccaa aagcaaaaat tgatgccaat aaagacgttg ataagcaagt
    29401 tcaagcatta attgacgaaa ttgatcgaaa tccaaatcta acagataagg aaaaacaagc
    29461 acttaaagat cgtattaatc aaatacttca acaaggtcat aacgacatta acaatgcgct
    29521 gactaaagaa gaaattgaac aagctaaagc acaacttgcg caagcattac aagacatcaa
    29581 agatttagtg aaagctaaag aagatgcgaa acaagatgtt gataaacaag ttcaagcatt
    29641 aattgacgaa atcgatcaaa atccaaatct aacagataag gaaaaacaag cacttaaaga
    29701 tcgtattaat caaatacttc aacaaggtca taacggcatt aacaatgcga tgactaaaga
    29761 agaaattgaa caagccaaag cacaacttgc acaagcatta aaagaaatta aagatttagt
    29821 gaaagctaaa gaaaatgcga aacaagatgt tgataaacaa gttcaagcat taattgacga
    29881 aatcgatcaa aatccaaatc taacagataa ggaaaaacaa gcgcttaaag atcgaatcaa
    29941 tcaaatactg caacaaggtc ataacgacat taacaatgcg atgactaaag aagaaattga
    30001 acaagccaaa gcacaacttg cacaagcatt acaagacatc aaagatttag tgaaagctaa
    30061 agaagatgcg aaaaatgcaa taaaagcctt agctaatgcg aagcgtgatc aaatcaattc
    30121 aaatccagat ttaacacctg agcaaaaagc aaaagcgctc aaagaaattg acgaagctga
    30181 aaaacgagca ctacaaaacg ttgagaatgc tcaaactata gatcaattaa atcgaggatt
    30241 aaacttaggt ttagatgaca ttagaaatac acatgtatgg gaggttgatg aacaacctgc
    30301 tgtaaatgaa atttttgaag caacacctga gcaaatccta gttaatggtg aactcattgt
    30361 acatcgtgat gacatcatta cagaacaaga tattcttgca cacataaact taattgatca
    30421 gctttcagca gaagttattg atacaccatc aactgcaacg atttctgata gcttaacagc
    30481 aaaagttgaa gttacattgc ttgatggatc aaaagtgatt gttaatgttc ctgtaaaagt
    30541 tgtagaaaaa gaattgtcag tagtcaaaca acaggcaatt gaatcaatcg aaaatgcggc
    30601 acaacaaaag attgatgaaa tcaataatag tgtgacatta acactggaac aaaaagaagc
    30661 tgcaattgca gaagttaata agcttaaaca acaagcaatt gatcatgtta acaatgcacc
    30721 tgatgttcat tcagttgaag aaattcaaca acaagaacaa gcgtatattg aacaatttaa
    30781 tccagaacaa tttacgattg aacaagcaaa atcaaatgca attaaatcga ttgaagatgc
    30841 aattcaacat atgattgatg aaatcaaagc tcgtactgat ctaacagata aagagaagca
    30901 agaagctatt gctaagttaa atcaattaaa agaacaagca attcaagcga ttcaacgtgc
    30961 gcaaagcatc agtgaaataa ctgagcaatt ggaacaattt aaagctcaaa tgaaagcagc
    31021 taatccaaca gcaaaagaac tagctaaacg caagcaagaa gctattagta gaattaaaga
    31081 cttttcaaat gaaaaaataa atagtattcg aaatagtgaa attggcacag ctgatgaaaa
    31141 acaagcagca atgaatcaaa ttaacgaaat tgtgcttgaa acaattagag atattaataa
    31201 tgcgcataca ttacagcaag ttgaggctgc attgaacaat ggtattgctc gaatttcagc
    31261 agtacaaatt gtaatatctg atcgtgctaa acaatcgtca agtactggaa atgaatctaa
    31321 tagccattta acaattggtt atggaactgc aaatcatcca tttaacagtt cgactattgg
    31381 acataaaaag aaacttgatg aagatgatga cattgatcca cttcatatgc gtcactttag
    31441 taataatttc ggtaatgtta ttaaaaacgc tattggtgtg gtgggtatct ctggcttact
    31501 agctagtttc tggttcttca ttgccaaacg tcgtcgtaaa gaagatgaag aggaagaatt
    31561 agaaataaga gataataata aagattcaat aaaagagact ttagacgata caaaacattt
    31621 accactttta tttgcgaaac gtcgcagaaa agaagatgaa gaagatgtta ctgttgaaga
    31681 aaaagattcg ctaaataatg gcgagtcact cgataaagtt aaacatacgc cgttcttctt
    31741 accaaaacgt cgtcgtaaag aagatgaaga agatgtggaa gttacaaatg aaaacacaga
    31801 tgaaaaagtg ttgaaagata acgaacattc accactctta ttcgcaaaac gacgcaaaga
    31861 taaagaggaa gatgttgaaa caacaactag tattgaatct aaagatgagg acgttccttt
    31921 attattggct aaaaagaaaa atcaaaaaga taaccaatcc aaagacaaaa agtcagcatc
    31981 aaaaaatact tctaaaaagg tagcagctaa aaagaagaaa aagaaatcta agaaaaataa
    32041 aaaataattt gtttctttga taaatagagg agcaccgatt gacatcacat cagtcggtgc
    32101 tccttttatt tattcttttt aattaattta tacaatgcct gttgagcgtg ttgattcgct
    32161 tctttgtttt gttctctcgg tatccattta acaaataata aatcaaaatc tttttcaaat
    32221 atttctattt gatcaaaata aggtttgaaa tttgcgtttt tcacataacc agcttcaatg
    32281 ctatctgcaa ttagctttga gtctgtatat aatagtgcgt tttgaacatt taattcacgt
    32341 gcatgttcta gtgcataaat acatgcagcc cattctgcag tgtggttatc catttcgcct
    32401 aactcatgtg tatatgtata atgctgctta tcttctttga ttacaatggc acatgtactt
    32461 atgcctggat ttcctttcgt cgcagcatca aaatttattt tcgccataat aaacctactt
    32521 tctattcaat acttagttaa agttactatt actgtaatac aaaatatgtt gggtaatcca
    32581 ttaaaaaaca cgcatcactt aaataagtaa cacgtgttta aaatactcgc tgattcaaag
    32641 atgattttct aatacgtagt actgtaatat acttcctaaa aaaatcatct tcaggctggg
    32701 acataaatca atgttctatg ctctacgaag ttatattggc agtagttgac tgaacgaaaa
    32761 tgcgcttgta acaagctttt ttcaattcta gtcaggggcc ccaacacaga agctgacgaa
    32821 aagtcagctt acaataatgt gcaagttggg gatggacccc aacaaagaga aattgtattc
    32881 ccaatttcta cagacaatgc aagttggggt gggacgacga aataaatttt gcgaaaatat
    32941 catttctgtc ccactccctt aaaacttatt cttttgtgta gtaagtgcgt taatagcctt
    33001 gatctaactt atcaatctta cctttacgat aaaatgattt agcaatatat ccaaatggta
    33061 cattgaaaac tgttgaagct aattttaata cgtaagttgt aataaatatt tcaaatacaa
    33121 ctgtaccagg taaacttccg ataaaagcga tagctacaaa taaagctgta tcaattattg
    33181 agcttaaaaa tgtactacca tatgcacgga tgaaaaacgt tttatcagaa ctaaatactt
    33241 ttttaattag tgaaaagata aatacatcaa tatgttgacc aataatatat gcgacaattg
    33301 agcctaaagc aatacgtggc acaacatcaa agattgcgtg taatgctttt tgtgccatat
    33361 cttctggtgc aggaataaaa tgtaatgaca attgcataac aataatcata attaatgttg
    33421 atgaaaagcc taaccaaact gctctttttg caactctacg cccataaata tcatttaaaa
    33481 tatctgttgc taaataaata gaagcaaaca tgacattacc taaagttgct gaaataccaa
    33541 agatttctac agttttaatc acttgtatgt tggcaatgat tgtgccaatt gcaacccatg
    33601 caattaaacc ttgtttacca aaaaagcgat acataagtac cataagtacg aacgttgcaa
    33661 taaacgtaac tagtcctaaa atttcattat acatattaaa atgtcctcct aaattttgat
    33721 catgcgggtg tttagaaacc gctcaataaa taacttactc attttactga aaaatcgttt
    33781 gcgatgcaat agcatttgaa tataaataca ttttacgtat agaattgtaa aaggtttcat
    33841 tcaaatctta gggtcaaaaa tgttataata tttttatgtc aaatttaaaa cagtaacact
    33901 tatttacaag gttgcaatat tttgaagtaa taaaggaagt gtcgcgtatt ttaacttttt
    33961 cagagcaaaa tgcactcgcg aaaatagatg atttaatgaa tacttattgc aatcaatgtc
    34021 caatcaaaac tcgtctgcgt aaattagagg ggaaaacgaa ggcgcatcat ttttgtatca
    34081 atgagtgttc aatagggaaa gaaataaaac aattaggaaa tgaacttcaa taggaggaag
    34141 tcaaatgaaa attatatcta tatcagaaac accgaaccac aacacaatga agattacact
    34201 tagtgaaagc agagaaggta tgacatcaga tacgtatact aaagttgatg attcacagcc
    34261 agcatttatt aatgacatct taaaggttga aggcgttaaa tcaattttcc atgttatgga
    34321 ctttatttca gtagataaag aaaatgacgc aaattgggaa acagtattgc caaaagtaga
    34381 ggctgtattc gaataaattt ttcatcaact agtattcggg gggaataaag tatatggaaa
    34441 ttttacgtat agagccaaca ccaagtccaa atacaatgaa agttgttttg tcatatacaa
    34501 gagaagacaa gttatctaat acttataaaa aagtagaaga aacacaacca agatttataa
    34561 atcagttgtt atctatagat ggtatcactt ccatttttca tgtcatgaac ttcttagctg
    34621 ttgataaggc accaaaagct gattgggaag tcatattacc tgatattaaa gctgcttttt
    34681 ctgatgcgaa taaggtttta gaatctgtaa atgaacctca aattgacaat cattttggtg
    34741 aaattaaagc tgaattatta acttttaagg gtataccgta tcaaattaag ctaacttctg
    34801 ctgaccaaga attaagagaa caattaccac aaacatatgt tgatcatatg actcaagcgc
    34861 aaacagcaca tgacaatatt gtttttatgc gtaaatggct agatttagga aatcgctatg
    34921 gaaatattga agaagtaatg gatggtgtcc tagaagaagt gctagctacc tatccagaat
    34981 cacagttacc cgtattggta aaacatgctt tagaagaaaa tcacgcaact aataattatc
    35041 atttctatcg acatgtctct ttggatgaat atcatgcaac tgataattgg aagactcgat
    35101 tacgaatgtt aaaccatttt ccaaagccga cttttgaaga tataccgcta cttgatttag
    35161 ctttatctga tgaaaaagta ccggttagac gtcaagcgat tgtattatta ggtatgattg
    35221 aaagtaaaga aattttaccg tatttatata aggggcttcg tgataaaagt cctgctgtaa
    35281 gaagaacagc aggggattgc ataagcgatt tagggtatcc agaggcacta ccagaaatgg
    35341 tgctactatt agatgatcca cagaaaatcg ttaggtggcg tgctgctatg tttatctttg
    35401 atgaaggtaa tgcagagcag cttcccgcac taaaagccca tattaatgac aatgcgtttg
    35461 aagttaaatt acaaattgaa atggccatat ctcgtattga aaacggagat gaggcacttg
    35521 gttcagtatg gaaacaaatg gcaaacagaa caatttaatt ggaggaatta aatatgaatg
    35581 catatgatgc ttatatgaaa gaaattgcgc aacaaatgcg tggcgaatta actcaaaatg
    35641 gttttacaag tttagaaacg agcgaagcag tatcggagta tatgaaccaa gtaaatgctg
    35701 atgacactac ttttgtagtt attaactcta catgcggctg tgcagctgga ttagcaagac
    35761 cagctgcagt agcagttgca acacaaaatg aacatagacc tacaaataca gttacagttt
    35821 ttgctgggca agataaagaa gcaactgcta caatgcgaga attcattcag caagcaccat
    35881 ctagtccttc gtatgcttta ttcaaaggtc aagatttagt ttattttatg cctagagaat
    35941 ttatcgaagg tagagatatt aatgacattg caatggactt aaaggatgcc tttgacgaaa
    36001 attgtaaata gtacacataa ataaatataa aggttaacac attttataat attaaaaatg
    36061 g
//
LOCUS       DAHHSC010000008        32773 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.008, whole genome shotgun sequence.
ACCESSION   DAHHSC010000008 DAHHSC010000000
VERSION     DAHHSC010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 32773)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32773)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32773
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.008"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            complement(<1..211)
                     /locus_tag="NBI57_000301"
     CDS             complement(<1..211)
                     /locus_tag="NBI57_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001656966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1821288.1"
                     /translation="MSDNLSLFIDYINDNIIYGSEIKREKLENLFNQFAIKNVEKNIV
                     YDELKSLDITIIESQDSYKNKLKRLF"
     gene            complement(626..1882)
                     /locus_tag="NBI57_000302"
     CDS             complement(626..1882)
                     /locus_tag="NBI57_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001569336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3578 domain-containing protein"
                     /protein_id="HCD1821289.1"
                     /translation="MIDNFMQVLKLIKEKRTNNVVKKSDWDKGDLYKTLVHDKLPKQL
                     KVHIKEDKYSVVGKVATGNYSKVPWISIYDENITKETKDGYYLVYLFHPEGEGIYLSL
                     NQGWSKISDMFPRDKNAAKQRALTLSSELNKYITSNEFNTGRFYYAENKDSSYDLKND
                     YPSGYSHGSIRFKYYDLNEGFTEEDMLEDLKKFLELFNELASKVTKTSYDSLVNSIDE
                     IQEDSEIEEIRIAQKDKTLKEVEAPKGIIPKYKKGVSKTTKNDSEIEKSNKENKLTGK
                     VGEKLALNYFNELIDNKIDEDKKEQFRNILNDNPGSQHGHGYDLVAFDPTNTDKAVEK
                     FIEIKTSTSSSIEEPFFMSLNEMFAMKEYKQKYLILRIFNVSGKEPQFYFIDPYANYS
                     EFKDVDDLIDKVFNVEAIQYKVFGEK"
     gene            complement(2204..2683)
                     /gene="rlmH"
                     /locus_tag="NBI57_000303"
     CDS             complement(2204..2683)
                     /gene="rlmH"
                     /locus_tag="NBI57_000303"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020363758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HCD1821290.1"
                     /translation="MKITILAVGKLKEKYWKQAIAEYEKRLGPYTKIDIIEVPDEKAP
                     ENMSDKEIEQVKEKEGQRILAKIKPQSTVITLEIQGKMLSSEGLAQELNQRMTQGQSD
                     FVFVIGGSNGLHKDVLQRSNYALSFSKMTFPHQMMRVVLIEQVYRAFKIMRGEAYHK"
     gene            complement(3052..5370)
                     /gene="adsA"
                     /locus_tag="NBI57_000304"
     CDS             complement(3052..5370)
                     /gene="adsA"
                     /locus_tag="NBI57_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored adenosine synthase AdsA"
                     /protein_id="HCD1821291.1"
                     /translation="MKALLLKTSVWLVLLFSVMGLWQVSNAAEQYTPIKAHVVTTIDK
                     ATTDKQQVTPTKEAAHQFGEEAATNVSASAQGTADEINNKVTSNAFSNKPSTAVSTKV
                     NETHDVDTQQASTQKPTQSATFTLSNAKTASLSPRMFAANVPQTTTHKILHTNDIHGR
                     LAEEKGRVIGMAKLKTIKEQEKPDLMLDAGDAFQGLPLSNQSKGEEMAKAMNAVGYDA
                     MAVGNHEFDFGYDQLKKLEGMLDFPMLSTNVYKDGKRAFKPSTIVTKNGIRYGIIGVT
                     TPETKTKTRPEGIKGVEFRDPLQSVTAEMMRIYKDVDTFVVISHLGIDPSTQETWRGD
                     YLVKQLSQNPQLKKRITVIDGHSHTVLQNGQIYNNDALAQTGTALANIGKVTFNYRNG
                     EVSNIKPSLINVKDVENVTPNKALAEQINQADQTFRAQTAEVIIPNNTIDFKGERDDV
                     RTRETNLGNAIADAMEAYGVKNFSKKTDFAVTNGGGIRASIAKGKVTRYDLISVLPFG
                     NTIAQIDVKGSDVWTAFEHSLGAPTTQKDGKTVLTANGGLLHISDSIRVYYDMNKPSG
                     KRINAIQILNKETGKFENIDLKRVYHVTMNDFTASGGDGYSMFGGPREEGISLDQVLA
                     SYLKTANIAKYDTTEPQRMLLGKPAVSEQPAKGQQGSKGSESGKDVQPIGDDKAMNPA
                     KQPATGKVVLLPTHRGTVSSGTEGSGRTLEGATVSSKSGNQLVRMSVPKGSAHEKQLP
                     KTGTNQSSSPAAMFVLVAGIGLIATVRRRKAS"
     gene            complement(5597..6397)
                     /locus_tag="NBI57_000305"
     CDS             complement(5597..6397)
                     /locus_tag="NBI57_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HCD1821292.1"
                     /translation="MSRLIRMSVLASGSTGNATFVENEKGSLLVDVGLTGKKMEELFS
                     QIDRNIQDLNGILVTHEHIDHIKGLGVLARKYQLPIYANEKTWQAIEKKDSRIPMDQK
                     FIFNPYETKSIAGFDVESFNVSHDAIDPQFYIFHNNYKKFTILTDTGYVSDRMKGMIR
                     GSDAFIFESNHDVDMLRMCRYPWKTKQRILGDMGHVSNEDAGHAMTDVITGNTKRIYL
                     SHLSQDNNMKDLARMSVGQVLNEHDIDTEKEVLLCDTDKAIPTPIYTI"
     gene            complement(6786..7574)
                     /locus_tag="NBI57_000306"
     CDS             complement(6786..7574)
                     /locus_tag="NBI57_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system regulatory protein YycI"
                     /protein_id="HCD1821293.1"
                     /translation="MNWKLTKTLFIFVFILVNIVLVSIYVNKVNRSHINEVESNNEVN
                     FQQEEIKVPASILNKSVKGIKLEQITGRSKDFSSKAKGDSDLTTSDGGKLLNANISQS
                     VKVSDNNLKDLKDYVNKRVFKGSEYQLSEINSGSVKYEQTYDNFPILNNSKAMLNFNI
                     EDNKAASYKQSMMDDIKPTDGADKKHQVIGVRKAIEALYYNRYLKKGDEVINARLGYY
                     SVVNETNVQLLQPNWEIKVKHDGKDKTNTYYVEATNNNPKIINH"
     gene            complement(7575..8909)
                     /locus_tag="NBI57_000307"
     CDS             complement(7575..8909)
                     /locus_tag="NBI57_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001060131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1821294.1"
                     /translation="MNNKEHIKSVILALLVLMSVVLTYMVWNFSPDIANVDNTDSKKS
                     ETKPLTTPMTAKMDTTITPFQIIHSKNDHPEGTIATVSNVNKLTKPLKNKEVKSVEHV
                     RRDHNLMIPDLSSDFTLFDFTYDLPLSTYLGQVLNMNAKVPNHFNFNRLVIDHDADDN
                     IVLYAISKDRHDYVKLTTTTKNDHFLDALAAVKKDMQPYTDIITNKDTIDRTTHVFAP
                     SKPEKLKTYRMVFNTISVEKMNAILFDDSTIVRSSKSGVTTYNNNTGVANYNDKNEKY
                     HYKNLSEDEASSSKMEETIPGTFDFINGHGGFLNEDFRLFSTNNQSGELTYQRFLNGY
                     PTFNKEGSNQIQVTWGEKGVFDYRRSLLRTDVVLNSEDNKTLPKLESVRSSLANNSDI
                     NFEKVTNIAIGYEMQDNSDHNHIEVQINSELVPRWYVEYDGEWYVYNDGRLE"
     gene            complement(8902..10728)
                     /gene="walK"
                     /locus_tag="NBI57_000308"
     CDS             complement(8902..10728)
                     /gene="walK"
                     /locus_tag="NBI57_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     WalK"
                     /protein_id="HCD1821295.1"
                     /translation="MKWLKQLQSLHTKLVIVYVLLIIIGMQIIGLYFTNNLEKELLDN
                     FKKNITQYAKQLEISIEKVYDEKGSVNAQKDIQNLLSEYANRQEIGEIRFIDKDQIII
                     ATTKQSNRSLINQKANDSSVQKALSLGQSNDHLILKDYGGGKDRVWVYNIPVKVDKKV
                     IGNIYIESKINDVYNQLNNINQIFIVGTAISLLITVILGFFIARTITKPITDMRNQTV
                     EMSRGNYTQRVKIYGNDEIGELALAFNNLSKRVQEAQANTESEKRRLDSVITHMSDGI
                     IATDRRGRIRIVNDMALKMLGMAKEDIIGYYMLSVLSLEDEFKLEEIQENNDSFLLDL
                     NEEEGLIARVNFSTIVQETGFVTGYIAVLHDVTEQQQVERERREFVANVSHELRTPLT
                     SMNSYIEALEEGAWKDEELAPQFLSVTREETERMIRLVNDLLQLSKMDNESDQINKEI
                     IDFNMFINKIINRHEMSAKDTTFIRDIPKKTIFTEFDPDKMTQVFDNVITNAMKYSRG
                     DKRVEFHVKQNPLYNRMTIRIKDNGIGIPINKVDKIFDRFYRVDKARTRKMGGTGLGL
                     AISKEIVEAHNGRIWANSVEGQGTSIFITLPCEVIEDGDWDE"
     gene            complement(10741..11442)
                     /locus_tag="NBI57_000309"
     CDS             complement(10741..11442)
                     /locus_tag="NBI57_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCD1821296.1"
                     /translation="MARKVVVVDDEKPIADILEFNLKKEGYDVYCAYDGNDAVDLIYE
                     EEPDIVLLDIMLPGRDGMEVCREVRKKYEMPIIMLTAKDSEIDKVLGLELGADDYVTK
                     PFSTRELIARVKANLRRHYSQPAQDTGNVTNEITIKDIVIYPDAYSIKKRGEDIELTH
                     REFELFHYLSKHMGQVMTREHLLQTVWGYDYFGDVRTVDVTIRRLREKIEDDPSHPEY
                     IVTRRGVGYFLQQHE"
     gene            complement(12062..12134)
                     /locus_tag="NBI57_000310"
     tRNA            complement(12062..12134)
                     /locus_tag="NBI57_000310"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12099..12101),aa:Asp,seq:gtc)
     gene            complement(12142..12216)
                     /locus_tag="NBI57_000311"
     tRNA            complement(12142..12216)
                     /locus_tag="NBI57_000311"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12181..12183),aa:Glu,seq:ttc)
     gene            complement(12645..13928)
                     /locus_tag="NBI57_000312"
     CDS             complement(12645..13928)
                     /locus_tag="NBI57_000312"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HCD1821297.1"
                     /translation="MSSIVVVGTQWGDEGKGKITDFLAEQSDVIARFSGGNNAGHTIQ
                     FGGETYKLHLVPSGIFYKDKLAVIGNGVVVDPVALLKELDGLNERGIPTSNLRISNRA
                     QVILPYHLAQDEYEERLRGDNKIGTTKKGIGPAYVDKVQRIGIRMADLLEKETFERLL
                     KSNIEYKQAYFKGMFNETCPSFDDIFEEYYAAGQRLKEFVTDTSKILDDAFVADEKVL
                     FEGAQGVMLDIDHGTYPFVTSSNPIAGNVTVGTGVGPTFVSKVIGVCKAYTSRVGDGP
                     FPTELFDEDGHHIREVGREYGTTTGRPRRVGWFDSVVLRHSRRVSGITDLSINSIDVL
                     TGLDTVKICTAYELDGKEITEYPANLDQLKRCKPIFEELPGWTEDVTSVRTLEELPEN
                     ARKYLERISELCNVQISIFSVGPDREQTNLLKELW"
     gene            complement(14206..15606)
                     /gene="dnaB"
                     /locus_tag="NBI57_000313"
     CDS             complement(14206..15606)
                     /gene="dnaB"
                     /locus_tag="NBI57_000313"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HCD1821298.1"
                     /translation="MDRMYEQNQMPHNNEAEQSVLGSIIIDPELINTTQEVLLPESFY
                     RGAHQHIFRAMMHLNEDNKEIDVVTLMDQLSTEGTLNEAGGPQYLAELSTNVPTTRNV
                     QYYTDIVSKHALKRRLIQTADSIANDGYNDELELDAILSDAERRILELSSSRESDGFK
                     DIRDVLGQVYETAEELDQNSGQTPGIPTGYRDLDQMTAGFNRNDLIILAARPSVGKTA
                     FALNIAQKVATHEDMYTVGIFSLEMGADQLATRMICSSGNVDSNRLRTGTMTEEDWSR
                     FTIAVGKLSRTKIFIDDTPGIRINDLRSKCRRLKQEHGLDMIVIDYLQLIQGSGSRAS
                     DNRQQEVSEISRTLKALARELECPVIALSQLSRGVEQRQDKRPMMSDIRESGSIEQDA
                     DIVAFLYRDDYYNRGGDEDDDDDGGFEPQTNDENGEIEIIIAKQRNGPTGTVKLHFMK
                     QYNKFTDIDYAHADMM"
     gene            complement(15638..16084)
                     /gene="rplI"
                     /locus_tag="NBI57_000314"
     CDS             complement(15638..16084)
                     /gene="rplI"
                     /locus_tag="NBI57_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HCD1821299.1"
                     /translation="MKVIFTQDVKGKGKKGEVKEVPVGYANNFLLKKNYAVEATPGNL
                     KQLELQKKRAKQERQQEIEDAKALKETLSNIEVEVSAKTGEGGKLFGSVSTKQIAEAL
                     KAQHDIKIDKRKMDLPNGIHSLGYTNVPVKLDKEVEGTIRVHTVEQ"
     gene            complement(16081..18048)
                     /gene="gdpP"
                     /locus_tag="NBI57_000315"
     CDS             complement(16081..18048)
                     /gene="gdpP"
                     /locus_tag="NBI57_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP phosphodiesterase GdpP"
                     /protein_id="HCD1821300.1"
                     /translation="MNRQSTKKALLIPFVIMIITAIVLMGVWFIFNSLIALIASIVLV
                     VMIIVSIILFRQALMKMDSYVDGLSAQISTTNNKAIKHLPIGIIVLDENDHIEWVNQF
                     MTNHMEANVISESVNEVFPNILKQLDRVKSVEIEYNQYHFQVRYSENDHCLYFFDITE
                     QVQTNELYENSKPIIATLFLDNYDEITQNMNDTQRSEINSMVTRVISRWATEYNIFFK
                     RYSSDQFVAYLNQKILADLEESKFDILSQLREKSVGYRAQLTLSIGVGEGTENLIDLG
                     ELSQSGLDLALGRGGDQVAIKSINGNVRFYGGKTDPMEKRTRVRARVISHALKDILAE
                     GDKVIIMGHKRPDLDAIGAAIGVSRFAMMNNLEAYIVLNETDIDPTLRRVMNEIDKKS
                     ELRERFITSDDAWDMMTSKTTVVIVDTHKPELVLDENVLNKANRKVVIDHHRRGESFI
                     SNPLLIYMEPYASSTAELVTELLEYQPTEQRLTRLESTVMYAGIIVDTRNFTLRTGSR
                     TFDAASYLRAHGADTILTQHFLKDDVDTYINRSELIRTVKVEDNGIAIAHGSDDKIYH
                     PVTVAQAADELLSLEGIEASYVVARREDNLIGISARSLGSVNVQLTMEALGGGGHLTN
                     AATQLKGVTVEEAIAQLQQAITEQLSRSEDA"
     gene            complement(18063..19001)
                     /locus_tag="NBI57_000316"
     CDS             complement(18063..19001)
                     /locus_tag="NBI57_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YybS family protein"
                     /protein_id="HCD1821301.1"
                     /translation="MGVAYVFSKIQPKATIIATITLVFVALALYLVPGLGLIFALFAT
                     IPGIVLWNKSIQSFGVSALITVIITTVLGNTFVLSAIILVLIASLIIGQLLKERTSKE
                     RMLYVTTVAMSLISLIAFMLLQTFGRIPPSASIVKPFKQTLHEAITMSGADANMTQIL
                     EEGFRQATVQLPGFIIIITFLIVLINLIVTFPVLRKFKIATPVFKPLFAWQMSGILLW
                     IYIIVIICLLFTGQPSVFQSILLNFQLVLSLVMYIQGLSVIHFFGKAKGLPNAVTILL
                     LIIGTILTPTTHIVGLLGVIDLSLNLKRIMKNNSKK"
     gene            complement(19294..20262)
                     /locus_tag="NBI57_000317"
     CDS             complement(19294..20262)
                     /locus_tag="NBI57_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HCD1821302.1"
                     /translation="MTNYTVDTLNLGKFITESGEVIDNLRLRYEHVGYHGQPLVVVCH
                     ALTGNHLTYGTDDYPGWWREIIDGGYIPIHDYQFLTFDVIGSPFGSSSPLNDPHFPKK
                     LTLRDIVRANERGIQALGYDKINILIGGSLGGMQAMELLYNQQFEVDKAIILAATSRT
                     SSYSRAFNEIARQAIHLGGKEGLSIARQLGFLTYRSSKSYDERFTPDEVVAYQQHQGN
                     KFKEHFDLNCYLTLLDVLDSHNIDRGRTDVTHIFKNLETKVLTMGFIDDLLYPDDQVR
                     ALGERFKYHRHFFVPDNVGHDGFLLNFSTWAPNLYHFLNLKHFKRK"
     regulatory      complement(20322..20420)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            complement(20625..20954)
                     /locus_tag="NBI57_000318"
     CDS             complement(20625..20954)
                     /locus_tag="NBI57_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HCD1821303.1"
                     /translation="MITHMNMLILILLCGIVTLLIRIIPFIMISKVQLPDVVVRWLSF
                     IPITLFTALVIDSIIQQTPHGEGYTLNIPYIIALIPTVILSIITRSLTITIISGIVIM
                     ATLRFFF"
     gene            complement(20951..21646)
                     /locus_tag="NBI57_000319"
     CDS             complement(20951..21646)
                     /locus_tag="NBI57_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HCD1821304.1"
                     /translation="MATHLSFRQGVQECIPTLLGYAGVGISFGIVASSQNFSILEIIL
                     LCLVIYAGAAQFIMCALFIAGTPISAIVLTVFIVNSRMFLLSMSLAPNFKTYGFWNRV
                     GLGSLVTDETFGVAITPYLKGEAINDRWMHGLNITAYLFWAISCVAGALFGEYISNPQ
                     TLGLDFAITAMFIFLAIAQFESITKSRLRIYIVLIIAVIVMMLSLSMFMPSYLAILIA
                     ATISAALGVMMER"
     gene            complement(22297..23583)
                     /gene="serS"
                     /locus_tag="NBI57_000320"
     CDS             complement(22297..23583)
                     /gene="serS"
                     /locus_tag="NBI57_000320"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HCD1821305.1"
                     /translation="MLDIRLFRNEPDTVKSKIELRGDDPKVVDEILELDEQRRKLISA
                     TEEMKARRNKVSEEIALKKRNKENADDVIAEMRTLGDDIKEKDSQLNEIDNKMTGILC
                     RIPNLISDDVPQGESDEDNVEVKKWGTPREFSFEPKAHWDIVEELKMADFDRAAKVSG
                     ARFVYLTNEGAQLERALMNYMITKHTTQHGYTEMMVPQLVNADTMYGTGQLPKFEEDL
                     FKVEKEGLYTIPTAEVPLTNFYRNEIIQPGVLPEKFTGQSACFRSEAGSAGRDTRGLI
                     RLHQFDKVEMVRFEQPEDSWNALEEMTTNAEAILEELGLPYRRVILCTGDIGFSASKT
                     YDIEVWLPSYNDYKEISSCSNCTDFQARRANIRFKRDKAAKPELAHTLNGSGLAVGRT
                     FAAIVENYQNEDGTVTIPEALVPFMGGKTQISKPVK"
     misc_binding    complement(23673..23889)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(23962..25476)
                     /gene="hutH"
                     /locus_tag="NBI57_000321"
     CDS             complement(23962..25476)
                     /gene="hutH"
                     /locus_tag="NBI57_000321"
                     /EC_number="4.3.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ammonia-lyase"
                     /protein_id="HCD1821306.1"
                     /translation="MTLYLDGETLTIEDIKSFLQQQSKIEIIDDALERVKKSRAVVER
                     IIENEETVYGITTGFGLFSDVRIDPTQYNELQVNLIRSHACGLGEPFSKEVALVMMIL
                     RLNTLLKGHSGATLELVRQLQFFINERIIPIIPQQGSLGASGDLAPLSHLALALIGEG
                     KVLYRGEEKDSDDVLRELNRQPLNLQAKEGLALINGTQAMTAQGVISYIEAEDLGYQS
                     EWIAALTHQSLNGIIDAYRHDVHSVRNFQEQINVAARMRDWLEGSTLTTRQAEIRVQD
                     AYTLRCIPQIHGASFQVFNYVKQQLEFEMNAANDNPLIFEEANETFVISGGNFHGQPI
                     AFALDHLKLGVSELANVSERRLERLVNPQLNGDLPAFLSPEPGLQSGAMIMQYAAASL
                     VSENKTLAHPASVDSITSSANQEDHVSMGTTAARHGYQIIENARRVLAIECVIALQAA
                     ELKGVEGLSPKTRRKYEEFRSIVPSITHDRQFHKDIEAVAQYLKQSIYQTTACH"
     gene            25802..26614
                     /locus_tag="NBI57_000322"
     CDS             25802..26614
                     /locus_tag="NBI57_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002461649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HCD1821307.1"
                     /translation="METLNSINIPKRKEDSHKGDYGKILLIGGSANLGGAIMLAARAC
                     VFSGSGLITVATHPTNHSALHSRCPEAMVIDINDTKMLTKMIEMTDSILIGPGLGVDF
                     KGNNAITFLLQNIQPHQNLIVDGDAITIFSKLKPQLPTCRVIFTPHLKEWERLSGIPI
                     EEQTYERNREAVDRLGATVVLKKHGTEIFFKDEDFKLTIGSPAMATGGMGDTLAGMIT
                     SFVGQFDNLKEAVMSATYTHSFIGENLAKDMYVVPPSRLINEIPYAMKQLES"
     gene            complement(26702..29371)
                     /gene="gyrA"
                     /locus_tag="NBI57_000323"
     CDS             complement(26702..29371)
                     /gene="gyrA"
                     /locus_tag="NBI57_000323"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HCD1821308.1"
                     /translation="MAELPQSRINERNITSEMRESFLDYAMSVIVARALPDVRDGLKP
                     VHRRILYGLNEQGMTPDKSYKKSARIVGDVMGKYHPHGDSSIYEAMVRMAQDFSYRYP
                     LVDGQGNFGSMDGDGAAAMRYTEARMTKITLELLRDINKDTIDFIDNYDGNEREPSVL
                     PARFPNLLANGASGIAVGMATNIPPHNLTELINGVLSLSKNPDISIAELMEDIEGPDF
                     PTAGLILGKSGIRRAYETGRGSIQMRSRAVIEERGGGRQRIVVTEIPFQVNKARMIEK
                     IAELVRDKKIDGITDLRDETSLRTGVRVVIDVRKDANASVILNNLYKQTPLQTSFGVN
                     MIALVNGRPKLINLKEALVHYLEHQKTVVRRRTQYNLRKAKDRAHILEGLRIALDHID
                     EIISTIRESDTDKVAMESLQQRFKLSEKQAQAILDMRLRRLTGLERDKIEAEYNELLN
                     YISELETILADEEVLLQLVRDELTEIRDRFGDDRRTEIQLGGFEDLEDEDLIPEEQIV
                     ITLSHNNYIKRLPVSTYRAQNRGGRGVQGMNTLEEDFVSQLVTLSTHDHVLFFTNKGR
                     VYKLKGYEVPELSRQSKGIPVVNAIELENDEVISTMIAVKDLESEDNFLVFATKRGVV
                     KRSALSNFSRINRNGKIAISFREDDELIAVRLTSGQEDILIGTSHASLIRFPESTLRP
                     LGRTATGVKGITLREGDEVVGLDVAHANSVDEVLVVTENGYGKRTPVNDYRLSNRGGK
                     GIKTATITERNGNVVCITTVTGEEDLMIVTNAGVIIRLDVADISQNGRAAQGVRLIRL
                     GDDQFVSTVAKVKEDAEDETNEDEQSTSTVSEDGTEQQREAVVNDETPGNAIHTEVID
                     SEENDEDGRIEVRQDFMDRVEEDIQQSSDEDEE"
     gene            complement(29408..31342)
                     /gene="gyrB"
                     /locus_tag="NBI57_000324"
     CDS             complement(29408..31342)
                     /gene="gyrB"
                     /locus_tag="NBI57_000324"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HCD1821309.1"
                     /translation="MVTALSDVNNTDNYGAGQIQVLEGLEAVRKRPGMYIGSTSERGL
                     HHLVWEIVDNSIDEALAGYANKIEVVIEKDNWIKVTDNGRGIPVDIQEKMGRPAVEVI
                     LTVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSQDLEVYVHRNETIYHQAYKKGVPQF
                     DLKEVGTTDKTGTVIRFKADGEIFTETTVYNYETLQQRIRELAFLNKGIQITLRDERD
                     EENVREDSYHYEGGIKSYVELLNENKEPIHDEPIYIHQSKDDIEVEIAIQYNSGYATN
                     LLTYANNIHTYEGGTHEDGFKRALTRVLNSYGLSSKIMKEEKDRLSGEDTREGMTAII
                     SIKHGDPQFEGQTKTKLGNSEVRQVVDKLFSEHFERFLYENPQVARTVVEKGIMAARA
                     RVAAKKAREVTRRKSALDVASLPGKLADCSSKSPEECEIFLVEGDSAGGSTKSGRDSR
                     TQAILPLRGKILNVEKARLDRILNNNEIRQMITAFGTGIGGDFDLAKARYHKIVIMTD
                     ADVDGAHIRTLLLTFFYRFMRPLIEAGYVYIAQPPLYKLTQGKQKYYVYNDRELDKLK
                     SELNPTPKWSIARYKGLGEMNADQLWETTMNPEHRALLQVKLEDAIEADQTFEMLMGD
                     VVENRRQFIEDNAVYANLDF"
     gene            complement(31352..32464)
                     /gene="recF"
                     /locus_tag="NBI57_000325"
     CDS             complement(31352..32464)
                     /gene="recF"
                     /locus_tag="NBI57_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HCD1821310.1"
                     /translation="MKLNTLQLENYRNYDEVTLKCHPDVNILIGENAQGKTNLLESIY
                     TLALAKSHRTSNDKELIRFNADYAKIEGELSYRHGTMPLTMFITKKGKQVKVNHLEQS
                     RLTQYIGHLNVVLFAPEDLNIVKGSPQIRRRFIDMELGQISAVYLNDLAQYQRILKQK
                     NNYLKQLQLGQKKDLTMLEVLNQQFAEYAMKVTDKRAHFIQELESLAKPIHAGITNDK
                     EALSLNYLPSLKFDYAQNEAARLEEIMSILSDNMQREKERGISLFGPHRDDISFDVNG
                     MDAQTYGSQGQQRTTALSIKLAEIELMNIEVGEYPILLLDDVLSELDDSRQTHLLSTI
                     QHKVQTFVTTTSVDGIDHEIMNNAKLYRINQGEIIK"
     gene            complement(32461..32706)
                     /gene="yaaA"
                     /locus_tag="NBI57_000326"
     CDS             complement(32461..32706)
                     /gene="yaaA"
                     /locus_tag="NBI57_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HCD1821311.1"
                     /translation="MIILVQEVVVEGDINLGQFLKTEGIIESGGQAKWFLQDVEVLIN
                     GVRETRRGKKLEHQDRIDIPELPEDAGSFLIIHQGEQ"
BASE COUNT         9758 a         6452 c         4869 g        11694 t
ORIGIN      
        1 aaaataatct cttcaattta tttttatatg aatcctgtga ctcaatgatt gtaatatcta
       61 aagatttcag ttcatcatag acaatgttct tttcaacatt ttttatagca aattgattaa
      121 ataaattctc taatttctcc cgtttgattt cactaccata gattatatta tcattgatat
      181 agtcaatgaa taatgacaaa ttatcactca taacagtccc aaccccttta ttttgataga
      241 ctaattatct tcatcattgt aaaacaaatt acacccttta aatttaactc aacttaaata
      301 tcgacaaatt aaaaaacaat aaaattactt gaatattatt cataatatat taacaacttt
      361 attatactgc tctttatata taaaatcatt aataattaaa caagccttaa aatatttaac
      421 ttttttgtga ttattacaca ttatcttatc tgctctttat caccataaaa atagaaaaaa
      481 caagattcct aaagaatata ggaatcttgt ttcagactgt ggacaaactg attttttatc
      541 agttagctta tttagaaagt tttatttaaa ttacagtttc tatttttatt agatcacaat
      601 tttattttag ctcttgttca agtaatcatt tttcgccaaa aactttatac tgaatagctt
      661 ctacattaaa tactttgtca atgagatcat ctacatcttt aaattcagaa taatttgcat
      721 atggatctat aaaataaaat tgtggttctt taccggaaac attaaatatt cttaatatta
      781 aatatttctg cttatattct ttcatagcaa acatttcatt tagcgacata aaaaatggtt
      841 cctcaatact agaagatgta gatgttttaa tttcaataaa tttttctaca gctttatctg
      901 tatttgttgg atcaaaagct actaaatcat agccatgacc gtgttgagag cctggattat
      961 catttaaaat attcctaaac tgttctttct tatcttcgtc tattttatta tcaattagct
     1021 cattaaagta atttagcgct aatttttctc caactttacc ggttaattta ttctctttat
     1081 ttgatttttc aatttctgaa tcatttttag tagtctttga tacacctttt ttatattttg
     1141 gaattattcc tttaggtgct tccacttcct tgagtgtctt atctttttgt gctattctaa
     1201 tttcttcaat ttcgctgtct tcctgtattt cgtctatgct attgaccaag ctatcatagg
     1261 atgtttttgt aacttttgaa gctaattcat taaatagttc taaaaatttc tttaaatcct
     1321 ctagcatatc ttcttctgtg aatccttcat tcaaatcata atatttgaat cttattgatc
     1381 catgagaata tcctgatgga taatcatttt ttaaatcata agatgaatct ttattttctg
     1441 cgtaataaaa tcttccagta ttaaattcat ttgatgtaat atatttattg agttcggaag
     1501 ataaagttaa tgctctttgt tttgcagcat ttttatcccg cggaaacata tcacttatct
     1561 ttgaccatcc ttgattcaaa gataagtata tgccttctcc ttccggatga aaaagatata
     1621 ccaaataata tccatccttt gtttcttttg ttatattctc atcatatatt gaaatccaag
     1681 gaactttact atagttccca gtagcaacct tccctacaac tgaatattta tcttctttta
     1741 tatgcacttt taactgcttg ggtaacttat catggactaa agttttatat agatcacctt
     1801 tatcccaatc agatttttta actacattat tggtacgttt ctctttaatt aatttaagga
     1861 cctgcataaa gttgtctatc atttgaaatt ccctcctatt ataaaatata ttatgtctca
     1921 ttttcttcaa tatgtactta tttatatttt accgtaattt actatattta gttgcagaaa
     1981 gaattttctc aaagctagaa ctttgcttca ctataagtat tcagtataaa gaatatttcg
     2041 ctattattta cttgaaatga aagactgcgg aggctaacta tgtcaaaaat catgaacctc
     2101 attacttatg ataagcttct taaaaacata acagcaattc acataaacct catatgttct
     2161 gatacattca aaatcccttt atgaagcggc tgaaaaaacc gcatcattta tgatatgctt
     2221 ctccacgcat aatcttaaat gctctataca cttgctcaat taacacaacc cgcatcattt
     2281 gatgtgggaa tgtcattttg ctgaatgata gtgcgtagtt actgcgttgt aagacgtcct
     2341 tgtgcaggcc gtttgatccg ccaatgacga atacaaagtc gctttgccct tgggtcatgc
     2401 gttggttcaa ttcttgggcc aatccttcgg aagatagcat ctttccttgt atttctaatg
     2461 taatgactgt ggattgtggt ttaattttgg ctagtattcg ttggccttct ttttctttta
     2521 cttgctcaat ttctttgtcg ctcatatttt ctggtgcttt ttcgtctgga acttctatga
     2581 tgtctatctt ggtgtatggg cctaaacgtt tttcatattc tgctatggct tgcttccaat
     2641 atttctcttt tagtttccct acagctaaaa tggtgatttt catgtcgttt ggtcctccaa
     2701 attgttatca actttccagt tatccacaag ttattaactt gttcacactg ttccctctta
     2761 ttataccaat attttttgca gtttttgata ttttcctgac atttatgccc aatttttagt
     2821 gtataacttg tgtatttgtg gataactctg tgggcattgt gggtatttct ctttaaattg
     2881 tacacaattc tggataacta tccccatttc tgtggataaa caccttgatg tcttattact
     2941 tatccccgtt tttatatccc atgtttatca acgtttatag cttttttcca taaaaaaaac
     3001 actaccgtac ctcttattta agaaatacag tagtattgtt ttcaatatat tttaactagc
     3061 ttttctacgt cgtacagtcg cgattaaacc tatacctgct actaatacaa acatcgctgc
     3121 tgggcttgag ctttgattag ttccagtttt tggtaactgt ttctcatgcg cgctaccttt
     3181 aggcactgac attctaacca attggttccc actcttgctt gatacagtag ctccttctaa
     3241 tgtgcgacca gaaccttctg taccgctact aacagttcct ctatgcgttg gtaacaatac
     3301 aactttacct gtcgctggtt gtttcgctgg attcatcgct ttgtcgtcac caattggttg
     3361 tacatcttta ccagactcac tacctttgct accttgttgt cctttagctg gttgttcact
     3421 tactgctggt ttacctaata acatacgttg tggttctgtc gtatcatact tagctatgtt
     3481 agctgttttt aaataacttg ctagtacttg atctaatgaa ataccttctt ctctagggcc
     3541 accgaacata ctatatccgt cgccacctga tgctgtgaag tcattcatcg ttacatgata
     3601 tacacgtttt aaatcaatat tttcaaactt acctgtctct ttatttaaaa tttgaatagc
     3661 gttaattcgt ttgccagacg gtttattcat atcatagtaa acacgaattg aatcagagat
     3721 atgtagtaaa ccgccattcg ctgttaatac tgtcttaccg tctttttgtg ttgttggtgc
     3781 acctaaacta tgttcgaaag ctgtccagac gtctgaacct tttacatcaa tttgcgcaat
     3841 cgtatttcca aatggtaata ctgagattaa atcatagcgt gtcaccttac cttttgcgat
     3901 agaggcacga atacctccac catttgtcac ggcaaagtca gtctttttag agaaattctt
     3961 aacgccatac gcttccatag catctgcaat cgcgtttcct aaatttgttt cacgcgttct
     4021 aacgtcatct ctttctcctt tgaaatcaat ggtattattt ggaataataa cctctgctgt
     4081 ttgtgctcta aatgtttgat cagcttgatt aatttgttca gctaatgctt tgttcggtgt
     4141 tacattttca acgtctttaa cattaatcaa tgacggttta atatttgata cctctccatt
     4201 gcggtaatta aatgtaacct taccgatatt cgcaagtgct gtacctgttt gtgctaatgc
     4261 atcattgtta taaatttgac cattttgaag tacggtatgt gaatgaccat caatgactgt
     4321 aatacgtttc ttcaattgtg gattttgact taattgtttc actaagtaat caccacgcca
     4381 tgtttcttgt gttgaaggat caatccctaa atgtgatata acaacaaatg tatctacgtc
     4441 tttataaata cgcatcattt ctgctgtcac actttgtaat ggatctctaa attcaacacc
     4501 tttaatgccc tcaggtcttg ttttcgtctt tgtttctggt gtcgttacgc caataattcc
     4561 ataacgaata ccatttttcg ttacaattgt tgaaggctta aacgcgcgtt tcccatcttt
     4621 gtaaacgtta gtacttagca tcgggaagtc taacataccc tctaactttt tcaactgatc
     4681 gtatccaaag tcaaattcat ggttacccac tgccatagca tcataaccta ctgcattcat
     4741 tgctttagcc atttcttcac ctttagactg gtttgaaagt ggtaaacctt ggaaggcgtc
     4801 tcctgcgtct aacattaaat caggcttttc ttgttctttt attgttttta atttagccat
     4861 accgatgaca cgcccttttt cttcggctag tcggccatgg atatcatttg tatgtaatat
     4921 tttatgtgtt gttgtttgtg gtacattggc agcaaacatt cgtggtgaaa gtgatgctgt
     4981 tttagcattt gataatgtga atgttgctga ttgagttggt ttttgtgttg aggcttgttg
     5041 tgtatctaca tcgtgcgttt cgtttacttt tgttgaaact gctgtagatg gtttgttaga
     5101 aaatgcgttg gatgttactt tattgtttat ttcatcagct gttccctgtg ctgatgctga
     5161 tacgttggtt gccgcttctt caccaaattg atgagccgct tcctttgttg gcgttacttg
     5221 ttgcttatct gttgttgctt tgtctatcgt tgttactaca tgtgctttga ttggtgtata
     5281 ctgctcagcc gcgttcgaga cttgccataa tcccatcaca ctaaaaagca aaacgagcca
     5341 tacacttgtt ttaagtaata aagctttcat ttcgttccct ccattaactg tcatacacca
     5401 atatattatc ataatcctta tattaaaatg attgatttta tgtaaattat ttataaatta
     5461 catttaatat cgaattattt tttacatttc aaatagaacc ttaattccaa caacagccat
     5521 ttcccaccaa taacataaaa aagcacccga taaagtcgtc tcacagcagt gccgaacttt
     5581 atagggtgac tctcatttat attgtatata ttggcgttgg aatagcttta tccgtatcac
     5641 atagcaatac ttctttttcc gtatcaatat cgtgttcgtt caatacttgg ccaacactca
     5701 tacgcgccaa atctttcatg ttattgtctt gtgataaatg cgataggtaa atacgtttcg
     5761 tgttacctgt aatcacatct gtcatcgcat gacccgcatc ctcattagat acatgaccca
     5821 tatcgcctaa aatacgttgt ttcgtcttcc atggataacg acacattctc aacatatcga
     5881 cgtcatgatt actctcaaaa ataaacgcat cgctgccacg tatcatacct ttcatacgat
     5941 cagacacgta acccgtatcc gttaaaatcg taaacttctt atagttatta tggaaaatat
     6001 aaaattgcgg atctattgca tcatgtgaca cgttaaacga ttcaacatcg aaacctgcaa
     6061 tagattttgt ttcataagga ttaaaaatga atttctgatc catagggatg cgactatctt
     6121 tcttttcaat tgcctgccaa gtcttttcat tcgcataaat tggcaattga tatttacgcg
     6181 ccaaaacacc taatccttta atatgatcaa tatgttcatg ggttactaaa ataccattta
     6241 aatcttgaat attacggtca atttgactaa acaattcttc cattttcttg ccagtcaaac
     6301 caacatcaac taatagacta cctttttcat tttctacaaa agtggcgtta cctgtactac
     6361 cacttgctaa tacactcatg cgtatcaagc ggctcatcct ttctctttaa acagtcaatc
     6421 tctctatatg actgcaatat acatcagtgc aaatatacga ttttccctac agaaactatc
     6481 agtatattag ctgttttgta tacctttcct attatgtaac tttagttcat cgaccggttt
     6541 cttatattta aattttgata atcacgtgca atgaatttct taattattat aacaatgatt
     6601 agacaaatac taaagttttt aaaatagcag ttacgttaat tttaaaatgc ggttctactt
     6661 aaaatatgaa taaatgcgca cattagagac gcagaaatgt catccatgaa aacccatacc
     6721 agcaaaaagc tagcaccgct tctcacatat gatgagcttg caatactagc ttattacgat
     6781 tcatattaat gattaataat tttagggtta ttatttgtcg cttcgacata gtaagtattc
     6841 gttttgtcct taccgtcatg cttcacttta atttcccagt ttggttgtaa caattgaaca
     6901 ttcgtttcgt tcacgactga gtagtaaccg agtctagcat taatgacttc atcacctttt
     6961 ttcaagtaac gattataata taatgcctcg attgcttttc tcactccaat aacttgatgc
     7021 ttcttatctg caccatctgt gggcttaatg tcatccatca ttgattgttt ataactagcc
     7081 gctttgttat cttctatatt aaagttcaac atcgctttac tattgttcaa aatcgggaaa
     7141 ttatcatacg tttgttcgta ttttacagaa ccagaattaa tttcacttaa ttgatattct
     7201 gaacctttga acacacgttt gttaacataa tctttcaaat cttttaagtt attgtcactg
     7261 acctttaccg attgactaat gttcgcattc aataattttc caccatctga tgtggtcaaa
     7321 tccgaatcgc ccttagcttt agaactaaag tcttttgatc gccctgtaat ttgctctaat
     7381 tttatacctt taactgattt attcaatata ctagccggta ctttaatttc ttcttgctga
     7441 aaattaactt cattgttact ctcaacttca ttaatgtgtg agcgattgac tttattaaca
     7501 taaatcgata ctaacacgat gttgacaaga ataaacacga aaatgaaaag tgtctttgtc
     7561 agtttccagt tcatttattc aagcctccca tcgttataaa cataccattc accatcatat
     7621 tctacatacc aacgcggtac gagttcactg ttaatctgca cttcaatgtg attatgatct
     7681 gagttatcct gcatttcgta accgatagcg atgtttgtta ctttttcaaa attaatatca
     7741 ctattgttcg ctaagcttga acgtacagac tctaatttcg gcaacgtttt attatcctca
     7801 ctatttaaca caacgtcggt gcgcaataac gaacgacgat aatcaaagac gcctttttca
     7861 ccccaagtga cttgaatttg attagagcct tctttattaa acgttggata accgttaagg
     7921 aaacgttgat atgttaactc gcctgactga ttattcgtac taaacaatct gaagtcttcg
     7981 tttaagaaac caccatgacc attaataaaa tcaaaggttc ctggaatcgt ttcttccatt
     8041 ttgctggaac ttgcttcatc ttcggacagg tttttataat gatatttttc atttttatcg
     8101 ttatagtttg cgacacctgt attattgttg taggttgtaa caccactctt tgaactacga
     8161 acgatggttg aatcgtcaaa tagtatagca ttcattttct caacactaat cgtgttaaat
     8221 accatgcgat atgtttttaa cttttcaggt ttacttggtg caaaaacatg cgtcgtacga
     8281 tcaattgtat ctttgtttgt gatgatatct gtgtatggtt gcatatcttt tttcactgct
     8341 gctaatgcat ctaaaaaatg atcatttttc gttgtagttg ttaattttac gtaatcgtgg
     8401 cggtctttac ttatagcata aagcacgata ttatcatcag catcatgatc tatgactaaa
     8461 cgattgaaat tgaaatgatt tggtactttc gcattcatgt tcagtacttg gccaagatat
     8521 gttgataacg gtaaatcata cgtaaaatcg aataatgtaa aatcactgct taaatccgga
     8581 atcatcaagt tatgatcacg acgaacatgt tccacggact tcacttcttt atttttcaaa
     8641 ggtttcgtca gtttattcac attagatacc gtcgcaatcg taccttctgg atgatcattt
     8701 ttcgaatgaa taatctgaaa tggcgtaata gttgtatcca ttttggctgt cataggtgtc
     8761 gttaaaggtt tcgtttcact cttcttacta tctgtattgt cgacatttgc aatatcagga
     8821 gaaaagttcc ataccatata tgtcaatacg acactcatca agacgagtag cgctaaaatg
     8881 acagatttaa tatgttcctt attattcatc ccaatcaccg tcttcaatga cttcacatgg
     8941 aagtgtgata aagatagatg taccttgacc ttctacactg tttgcccaaa tacgaccatt
     9001 gtgcgcttcc acaatctctt tcgaaatggc tagtcctaat ccagtaccac ccattttacg
     9061 cgtacgtgcc ttatctacac gatagaatcg gtcgaatatc ttatcgactt tattgatagg
     9121 aataccgatg ccattatctt taatacgaat cgtcattcga ttataaagtg gattttgttt
     9181 cacgtggaac tcgacacgtt tatcgcctct agaatatttc atcgcatttg taatgacatt
     9241 atcgaatact tgcgtcattt tatcaggatc aaattctgtg aaaatcgtct ttttcggaat
     9301 atctcgaata aatgttgtgt ctttcgcaga catttcatgt cgattaataa ttttattaat
     9361 gaacatgtta aagtcgataa tttctttgtt gatttgatca gactcattat ccattttaga
     9421 taactgtagc aagtcattga ccagtcgaat cattcgttct gtttcttcac gggtaacaga
     9481 taaaaattgt ggcgcaagtt cctcatcttt ccatgcacct tcttcaagtg cttcaatgta
     9541 actattcata gaagttaaag gtgtacgtaa ctcatgtgat acattggcaa caaattcacg
     9601 acgctcacgt tcaacttgtt gttgttcagt aacgtcatgt aacacagcga tataaccagt
     9661 tacaaatcct gtttcctgca caatcgtact aaagttaaca cgtgcgatta gaccttcttc
     9721 ttcatttaaa tctaataaaa aactatcatt attctcttga atttcttcaa gtttaaattc
     9781 atcttcaaga cttaatacac ttaacatgta atatccgatg atgtcttctt tcgccatacc
     9841 aagcatcttg agtgccatat cattgacgat acgaatacgt ccacggcggt ctgttgcaat
     9901 aataccatca ctcatatggg tgataactga gtccagtcta cgtttctcac tttcagtatt
     9961 agcctgcgct tcttgtacac gtttagacaa gttattaaat gctaaagcta attcgccaat
    10021 ttcatcatta ccataaatct tcacacgttg cgtatagtta cctctggaca tttcgaccgt
    10081 ctggttacgc atatcggtga ttggtttggt aatcgttcgc gctataaaga atcctaggat
    10141 gactgtgatt aataatgaaa tagctgtacc aacaatgaat atttgattta tattatttaa
    10201 ttggttataa acgtcattaa tttttgattc gatataaata ttaccaatta cctttttatc
    10261 gactttaact gggatattat atacccagac acggtcctta ccaccgccat aatcttttaa
    10321 aattaaatga tcgtttgatt gtcctagtga tagtgctttt tggacagaac tatcattcgc
    10381 tttttgattg attagactac ggttagactg cttcgtcgtc gcaataataa tttggtcttt
    10441 atctataaaa cgaatttctc caatttcttg acggttggca tactcactta ataaattttg
    10501 aatatctttt tgtgcattta cggagccctt ttcgtcatat actttttcaa tactaatttc
    10561 taattgttta gcgtactgcg taatattctt cttaaaatta tcaagcagct ctttttcaag
    10621 attatttgta aaatacagcc cgataatttg cataccaatg ataatcagta atacataaac
    10681 aattacaagt ttagtatgaa gggattgtag ttgttttagc cacttcattc gtttcgacct
    10741 ctactcatgt tgttggagga aatatccaac gcctctacgc gtcacaatat attcaggatg
    10801 tgacggatca tcttcaatct tttcacgtaa acgacgaatc gttacatcga ccgtacgtac
    10861 atcgccaaag taatcatagc cccatactgt ttgtaataaa tgttcacgtg tcattacttg
    10921 tcccatatgt tttgataaat aatggaacaa ttcaaattca cgatgtgtta attcaatatc
    10981 ttcgccacgt tttttaatag aatatgcgtc tggataaatc acaatatctt taattgtgat
    11041 ttcattcgtt acatttccag tgtcttgtgc tggttgtgag taatgacgac gtaagttcgc
    11101 tttcacacgt gcgattaatt cacgcgtact aaacggtttc gttacatagt catctgcacc
    11161 tagttctaaa ccaagcactt tatcaatttc tgaatcttta gcagtaagca ttattattgg
    11221 catttcgtat tttttgcgca cttcacgaca tacttccata ccatcacgac caggtaacat
    11281 gatatctagt aatacgatgt ctggttcttc ttcataaatt aagtcgactg catcattacc
    11341 atcgtatgca cagtacacat cgtatccttc tttttttaag ttaaattcta aaatatcagc
    11401 aatcggtttt tcatcatcaa ctacaacaac ttttctagcc atttgcataa acctcttttc
    11461 ttaaatcatt aatattattt tatcattttt tgttctttat cgtttatgtt tgtgtaaaaa
    11521 atcacaggat gtcgcttact atttttgtta ttgtgcgcat tttccgtctt ataacgtata
    11581 gtaacctatt atataattta ccatttataa gctattaatt ctattttcca atataaactt
    11641 atatattaat attttttatt agtttctcac ggcggattat gataatgata tttaacacaa
    11701 tgatgaggtc tctattccaa taataaaatt agggtaatgg caacttctag ttgcacgtca
    11761 ttgctcagaa aataaatatc attattcgtt attcgttact ttaacaattg atgttatgga
    11821 ggattggtgg catatcacaa cgaagaaata tgttgacagt agcatatgtt tgtacgtcta
    11881 ataaagattt tattgtctac gtttatcttt catatccaac cactaagtta cttgaggttc
    11941 atacattata aagaaaatgg acgaacagat aaatcaatat gattatcatt cgtccatata
    12001 tcttttatta atataatatt aaacgcaaaa aaatgcctat cctcttggat aggcatttgt
    12061 acggtctcga cgggaatcga acccgcgatc tcctgcgtga caggcaggcg tgttaaccgc
    12121 tacactacga gacctaaaaa atggtgactc ctacgggact cgaacccgtg ttaccgccgt
    12181 gaaagggcgg tgtcttaacc gcttgaccaa ggagccgttc ttaagcacaa gatagattat
    12241 agcacagcca atcttgccat gacaagatat tttttacata attttgctat tattttttga
    12301 agaatgttat aagtgaaaaa ttgcatttta ttggcagtct atttattata ataattacgc
    12361 tatttttgaa atttttctat tttaggtact ttcatttaca cttacgtcta ctctatttta
    12421 atagctattt tcgacatcag acttattaaa ttcaagtatt tcttcttaat tttgttaaat
    12481 atcatctaat cttttaaatt atgactagca aatatcacct ttacgttagt aatttttatc
    12541 taaataatcg aatcgataca gaagcaaaca aagcaaaagc ctcctaccaa taaagataga
    12601 aggctcatgt atttataatc attgcgtatg acttatataa agttctacca caattctttt
    12661 aataggtttg tttgttctct atctggacca actgagaaga tagaaatttg tacattacat
    12721 aattctgaaa tacgctctaa atatttacgt gcattttcag gtaattcttc taaagtacgc
    12781 acacttgtta cgtcttctgt ccaacctggt aactcttcaa agattggttt acaacgtttt
    12841 aattgatcta agtttgctgg gtactcagta atttctttac cgtctaattc ataagctgta
    12901 cagattttca ctgtgtctag gcctgttaaa acatcgattg agttaataga taaatctgta
    12961 ataccactta cacgacgaga gtgacgtaat acaactgagt caaaccaacc tacacgacgt
    13021 ggacgtcctg ttgttgtacc gtattcacga ccaacttctc taatatgatg tccatcttca
    13081 tcgaataatt cagtagggaa tggaccatca ccaacacgtg atgtataagc tttacataca
    13141 ccaattacct tcgaaacgaa tgtaggacct acacctgtac caacagtaac gttacctgca
    13201 attggattac ttgatgtaac gaatggatat gtaccatggt cgatatctaa cattacacct
    13261 tgcgcacctt cgaaaagtac cttttcatct gctacaaatg catcgtctaa gatttttgat
    13321 gtgtctgtta caaattcttt taaacgttga cctgcagcat agtattcttc aaagatatca
    13381 tcaaatgatg gacatgtttc gttaaacata cctttgaaat atgcttgttt atattcaatg
    13441 tttgatttta ataatctttc gaatgtttct ttttcaagta aatctgccat acgaataccg
    13501 atacgttgaa ctttgtctac atatgctgga ccgatacctt ttttagttgt accaatctta
    13561 ttgtcaccgc gtaaacgttc ttcatattca tcttgtgcta agtgatatgg taaaatcact
    13621 tgcgcacgat tagatatacg taaattactt gtaggaatgc cacgttcatt taatccgtct
    13681 aattctttca atagtgcaac tggatcaaca acgacaccgt taccgattac cgctaatttg
    13741 tctttgtaaa agataccaga tggtactaaa tgtaatttat atgtttctcc gccaaattga
    13801 atggtatggc ctgcattatt accacctgaa aaacgcgcga taacatctga ctgttctgcc
    13861 aagaaatccg ttatttttcc ttttccttcg tctccccatt gtgtcccaac tactacgatt
    13921 gatgacatgt gagcacctcc aagtttttct catttaacca tttgatattc taccaatatc
    13981 gagaccataa tgcaaataaa aatcgaacat tgattttcgg cctttttaaa agaaaaatca
    14041 ttttaacaaa tcgtcttcaa cgtcctaaaa accctttaac agaggcgttg acagtactaa
    14101 tttatcactt tcaatacaca aacttaacat atatcaaccc gttcagaaca aaataaaaac
    14161 cgtacaattt tatcatctta atgattattg tacggaaaaa cttttttaca tcatatctgc
    14221 atgtgcataa tcgatatcgg taaatttatt atattgtttc ataaagtgta acttaactgt
    14281 gcctgttgga ccgttacgtt gcttagcaat gataatttca atttcaccgt tttcatcatt
    14341 cgtttgtggc tcgaaaccac catcatcgtc atcatcttca tcgccgccac ggttatagta
    14401 atcatcacgg tataagaatg caacgatatc ggcatcttgc tcaatcgaac cagattcacg
    14461 aatatcactc atcattggac gtttatcttg tcgttgttca acaccacgag ataactgact
    14521 taatgcgata actggacatt ctaattcacg ggctaatgct tttaatgtac gagagatttc
    14581 agaaacttcc tgttgtctgt tatcggacgc acgtgaacca ctaccttgaa tcaactgtaa
    14641 gtagtcaatc acaatcatgt ctaagccatg ttcttgcttt aatcgacgac atttagaacg
    14701 taaatcatta attcgaatac ccggtgtatc atcaataaaa atcttcgtac gtgataattt
    14761 acctaccgct atagtaaaac gactccaatc ttcctcagtc atagtacccg ttcttaagcg
    14821 gtttgagtca acattaccag aactacaaat catacgtgtg gctaactgat cagcacccat
    14881 ctctagtgag aaaataccaa ctgtatacat atcttcatgc gttgcaactt tttgtgcaat
    14941 attaagtgcg aacgcagtct tacctacaga tggacgcgct gcaaggataa ttaaatcatt
    15001 tcggttgaac cctgctgtca tttggtctaa atctcgatat cctgtaggta tacctggtgt
    15061 ttgaccacta ttttgatcaa gctcttcagc tgtttcatac acttgtccta agacgtctcg
    15121 aatgtcttta aagccatcgc tttcacgaga agatgatagc tctaaaattc gacgttctgc
    15181 atcacttaaa atcgcatcta gttcaagttc atcattatat ccatcattgg caatactatc
    15241 tgcagtttga atcaatctac gttttaatgc atgcttagaa acgatatcag tataatactg
    15301 aacatttcgc gtcgttggta catttgtaga taactctgca agatattgcg ggccacccgc
    15361 ttcattcaac gtaccttccg tcgataattg atccatcaat gttacaacat caatttcttt
    15421 attatcttca tttaagtgca tcattgcacg gaaaatatgt tgatgggcac ccctataaaa
    15481 cgactcagga agcaaaactt cctgagtagt attaatcaat tctggatcta taataattga
    15541 acctaagaca gactgttcag cttcattgtt atgtggcatt tgattttgct catacattct
    15601 atccatgaat ggttacacct cttatttcaa tccaacttta ttgttcaact gtgtgtacgc
    15661 gaattgtacc ttcaacttct ttatctaatt taacaggtac attcgtatat cctagggaat
    15721 gaattccatt tggtaaatcc attttacgtt tatcaatttt aatatcatgt tgtgctttta
    15781 gtgcttcggc aatttgtttt gtacttactg atccaaacaa tttaccacct tcaccagttt
    15841 ttgctgatac ttcaacttca atgtttgata acgtttcttt taatgcttta gcatcttcaa
    15901 tttcttgttg gcgttcttgt tttgcacgtt ttttctgtaa ctctaattgt ttaaggttac
    15961 ctggtgttgc ttctacagca taattctttt tcaataagaa gttatttgca taacctactg
    16021 gtacttcttt aacttcacct tttttacctt tacctttaac atcttgtgta aaaattactt
    16081 tcatgcatct tcactcctac ttaattgttc tgtaattgct tgttgtaatt gtgctatcgc
    16141 ctcttcgact gtcacacctt taagttgtgt tgcagcattg gttaaatgtc caccgccacc
    16201 aagtgcttcc attgttaact gaacatttac tgaaccgagt gaacgcgcag atataccaat
    16261 cagattatct tcacgtctcg caacaacata tgatgcttca ataccttcta aacttaacag
    16321 ttcatctgct gcttgtgcaa ctgttactgg atgataaatt ttatcgtctg aaccatgcgc
    16381 aatggctatg ccattatctt caacttttac agttcgaatt aattcagatc gattaatgta
    16441 agtatccaca tcatctttta agaaatgttg cgttaaaatc gtatctgcac catgtgcacg
    16501 taaataactc gctgcatcga atgttcttga tcctgttcgt aatgtaaagt ttcttgtatc
    16561 tacaataata cctgcataca tcactgttga ttcaagacgt gttaaacgtt gttctgttgg
    16621 ttgatattcc agtaactctg ttaccaattc agctgttgaa cttgcgtatg gttccatata
    16681 tatcaacaat ggattagaga tgaagctttc accacgtcta tgatgatcga taacaacttt
    16741 acggtttgct ttatttaaga cattttcatc taaaaccagt tccggtttat gcgtatcaac
    16801 aatcactacg gttgtcttag atgtcatcat atcccaagca tcatctgatg ttataaatcg
    16861 ctctcttaac tctgactttt tatcaatttc gttcatcacg cgtcgtaatg ttggatcaat
    16921 gtcagtctca tttaatacga tgtatgcttc taaattattc atcattgcaa atctagacac
    16981 accgattgct gcaccaattg catctaagtc aggacgttta tgtcccatga taatgacttt
    17041 gtcaccctct gcaaggatat cttttaacgc atgtgagatc actcgtgctc ttacacgagt
    17101 acgtttctcc atcgggtcag tcttaccgcc atagaaacgc acattaccat taatactttt
    17161 aattgcaact tggtcgccac cgcgccctaa tgctaagtct aggcctgatt gtgataattc
    17221 acctaaatcg attaaatttt cagtaccttc accaacacca atacttaatg ttaattgggc
    17281 acgataacca acactttttt cacgtaattg actcaagata tcaaatttag attcttctaa
    17341 gtcagctaat attttttgat ttaaataggc tacgaattga tcggaactat accttttaaa
    17401 gaatatatta tactcagttg cccatcgact aataacacgc gttaccattg aattgatttc
    17461 cgaacgctgc gtatcattca tattttgagt aatctcatcg tagttatcta aaaataatgt
    17521 cgcaatgatt ggtttagaat tttcatatag ttcatttgtt tgtacttgtt cagttatatc
    17581 aaagaaatag aggcaatgat cattctcaga ataacgtact tggaaatgat actgattata
    17641 ttctatttca acggatttca ctctatctaa ttgctttaaa atgtttggaa atacttcatt
    17701 tacagattca gaaatgacat ttgcttccat atgatttgtc ataaattggt taacccattc
    17761 gatgtgatca ttttcatcta aaacaatgat accaattggt aaatgtttga ttgctttatt
    17821 atttgttgtt gaaatttgag cactcaaacc atctacataa ctatccattt tcattaaagc
    17881 ttgtctgaat aaaatgatgc taacaataat catcacgaca agaacgatag atgcaattaa
    17941 tgctataaga ctattaaaga taaaccatac acccattaaa acaattgctg tgatgatcat
    18001 gatgacaaat ggtattagta aagctttctt agtggactgc cgattcatta ttccacctct
    18061 attcactttt tagaattatt tttcatgatt cgcttcaaat tcaaacttaa atcgataaca
    18121 ccaagtagtc ctacaatatg tgtcgtaggt gtcagtattg taccgataat caatagtaaa
    18181 atcgttactg cattcggcaa acctttcgct ttaccaaaga aatgaataac acttaaacct
    18241 tgaatataca ttactaatga taacacaagt tggaagttta aaagaatgct ctggaacaca
    18301 ctcggttgac ctgtaaataa taaacatatg ataacaataa tgtatatcca taataaaata
    18361 ccgctcattt gccacgcgaa aagtggctta aatacaggtg tagcgatttt aaattttcgt
    18421 aaaaccggaa atgtaacgat taagttaatt aagacgatta aaaatgtaat gataatgatg
    18481 aaacctggta attgaacggt cgcttgtcta aacccttctt ctaatatttg ggtcatattc
    18541 gcatcggcac cgctcatcgt aatcgcttca tgtaatgttt gcttgaaagg ttttactatg
    18601 ctcgctgatg gtggaatcct tccgaatgtt tgtagtaaca taaaagcgat taatgaaatt
    18661 aagctcatcg ctactgttgt tacgtataac attctttctt tagacgttct ttctttgagc
    18721 aattgaccaa taattaaact tgcaattaag actaatatga tggcacttaa aacgaaagta
    18781 ttacctaaaa cagttgttat aattactgta ataagtgcac taaccccgaa agattgtatt
    18841 gatttattcc ataaaacgat acctggtatg gttgcaaata atgcaaatat tagtcccaaa
    18901 ccaggcacta gatataaagc taaagcgaca aataccaacg taatcgttgc aattattgtt
    18961 gctttaggtt gtatttttga aaacacataa gccactccca tatttttaac tatagctatt
    19021 attttaacct ctttaatgga aattaacaat ttatagattg tatgcttcta tttcatttaa
    19081 ttgaataata actttcatgt tttataagta attaacatac tcatttgaat cgcttttgtg
    19141 tgctttcatt ttcaacatga ttatttaatc ccactacata gcaatcaagc ttgatttaga
    19201 tttacaattc atttccactc tcatgtactc tagatgtctt tgaatatgat aactgtgatt
    19261 tagtagcttc attctttaaa aatatataca ttattactta cgcttaaaat gctttaaatt
    19321 taagaaatga tataagttag gtgcccaggt actaaagttt agtaggaatc catcatgtcc
    19381 aacattatca ggcacgaaga aatgacgatg atatttaaaa cgttcaccta atgcacgaac
    19441 ttgatcgtcc ggatatagca aatcatctat gaaccccatc gttaagactt tcgtttctaa
    19501 atttttaaaa atatgcgtta cgtctgtgcg acctcggtca atgttgtgac tatccaatac
    19561 atctagcagt gtcagataac aattcaaatc aaaatgttct ttaaatttat taccttgatg
    19621 ttgttggtat gcgactactt catccggcgt gaaacgttca tcataacttt ttgatgatcg
    19681 atatgtcaaa aaacctaatt ggcgtgcaat acttagacct tccttaccac caagatgaat
    19741 ggcttgcctt gcaatttcat tgaaagctct actataagat gatgttcgac ttgttgcagc
    19801 aaggataatg gctttatcta cttcaaactg ttgattgtaa agtagttcca ttgcttgcat
    19861 acctccaaga cttcccccta ttaaaatatt aatcttatca taaccaaggg cttgtatacc
    19921 tcgttcattc gctctgacta tatctcttaa tgttaatttt ttaggaaaat gagggtcgtt
    19981 taaaggtgaa cttgaaccga aaggactacc aataacatca aatgttaaaa attgataatc
    20041 gtgaatgggt atatatcccc catcaataat ttctcgccac caacccggat aatcatctgt
    20101 tccatatgtt aaatgattgc cagttaatgc atgacaaact acaactaatg gttgtccatg
    20161 ataaccaaca tgctcatatc tcaaacgcaa gttatctatg acttccccag attctgtaat
    20221 aaatttccct agatttaaag tatctactgt gtaatttgtc attgttcttt cctccttaaa
    20281 caaaaaaact tctcacccta ttgaaaagta agaagtcttt atacttatca ttcgagtaac
    20341 tcgttggttt tagcaccgtg ctataaagtc ggttgctgaa gtatcacagg gccaagtccc
    20401 tcaacttctc acaataagaa atatgaaatt gttatgtgtt agttgagatt cagtgatgaa
    20461 ttacttttat catttaaaat attgttatca ttgtcatacg ttagccaaat cgctttcgtg
    20521 tatacgattc ccagtcatac catagacgat ttgtatatca gaattttctg attactaaca
    20581 gtttacctaa gtttaaatat ctgttcaatg attttcagtt atttttaaaa gaaaaatcgt
    20641 aatgttgcca tgataacaat cccactaata attgtaatag ttaaactacg cgtgattata
    20701 gataaaataa ccgtcggaat gagcgcgata atgtaaggga tgtttaatgt atacccctca
    20761 ccatgaggcg tctgttgaat aatgctgtca atgacaagtg ccgtaaatag tgtgattggg
    20821 ataaatgata gccatcgaac cacaacatca ggcaattgca cttttgaaat catgataaaa
    20881 ggtataattc gaattaatag cgttacgata ccacacaata aaataagtat taacatgttc
    20941 atatgagtta tcatcgttcc atcatcactc ctaacgctgc tgaaattgtg gctgcaatta
    21001 atattgctag atatgaaggc ataaacatac ttagcgataa catcattact atgacggcaa
    21061 taatgagtac tatgtaaatt cttaatcgcg atttagtaat tgattcaaat tgcgcaatgg
    21121 ccaaaaagat aaacatagcc gtgatagcaa aatctaaccc tagcgtttgc ggatttgaga
    21181 tatattcgcc aaataaagcc ccagctacac atgaaattgc ccaaaataaa tatgctgtga
    21241 tattaagacc atgcatccaa cgatcattga tagcttctcc ttttaaataa ggtgtaatgg
    21301 cgacgccaaa cgtttcgtca gttactaatg aacctaatcc aacacggttc caaaacccat
    21361 atgtcttgaa gtttggtgca agcgacatac ttaaaaggaa cattcttgaa tttacgataa
    21421 atacagttag tacaatcgct gatatcggtg tacctgctat aaacaacgcg cacataataa
    21481 attgcgcagc accggcatat ataacaagac ataacaagat aatttctaaa atactaaagt
    21541 tttgagacga agccacaata ccaaatgaaa taccaacacc ggcataaccc aataatgttg
    21601 ggatacactc ttgtacgcct tgtctaaaac ttaaatgtgt tgccatctca attacctcct
    21661 ttgccacttt atatttatat aattacgaaa aaataatata cctagtctaa tacagttcta
    21721 taccctaagc aataacatta gacaccagtt tgtctgaggt tagacatact ggcgtcttca
    21781 atcatcttca tatcaacttt ttatttttga taattttcaa tttttattta agactacctc
    21841 catattttct acggatttgt agttgttttt aagtactaat tttataattt tttatatcta
    21901 atgatgagtc tgggatattg attcatgtaa cactccctta taatcatcat cctctccccc
    21961 taccctactc catcgatatt actcatacta catcaacgaa atcagttttt tatcacttaa
    22021 tttcctataa tagtgatgct caaaattgtt acgttttaga ttgttttagt tcatcataat
    22081 tatcccgtat tgttgctata atgaaatgcg ttcaccccat taaaccacaa acttaattta
    22141 ttgttgttat gtgcattggc tcactattat acttttacag cacaaaaaaa gtggcgacag
    22201 cttcgtcacc actttttaaa atattattta aagtatcttg cccttgcttt aagtatacgt
    22261 agaatatata ctttttaaag cttgtagcta aagcctttat ttaactggtt ttgaaatttg
    22321 tgttttacca cccataaatg gtactaatgc ttctggaatt gttactgttc catcttcatt
    22381 ttggtaattt tcaacaatag cagcaaatgt acgtccaact gctaaaccac taccatttaa
    22441 tgtatgtgct aattctggtt tagctgcttt gtcacgcttg aagcggatgt tagcacgacg
    22501 cgcttggaaa tccgtacagt ttgagcatga actaatttct ttataatcat tgtagcttgg
    22561 taaccaaact tctatatcat atgttttgct tgcactaaat ccaatatcac ctgtacataa
    22621 aataacacga cggtatggta aacctaactc ttctagaatt gcttctgcat ttgttgtcat
    22681 ttcttctaaa gcattccatg aatcttcagg ttgttcaaaa cgtaccattt ccactttatc
    22741 gaattgatgt aaacgaatta atcctcttgt atctctacct gctgatcctg cttcactacg
    22801 gaaacatgca gattgaccag tgaatttttc aggaagtaca cctggttgaa taatttcatt
    22861 acggtagaaa ttcgttaatg gtacttcagc agttggaatt gtatataatc cttctttttc
    22921 tactttaaat aaatcttctt caaatttagg taattgacct gtaccataca ttgtatctgc
    22981 gttcacaagc tgtggtacca tcatttctgt ataaccatgt tgtgttgtat gttttgtaat
    23041 catatagttc attaaagcac gctctaattg cgcaccttca tttgttaaat atacaaaacg
    23101 cgcacctgaa acttttgctg cacgatcaaa atcagccatt ttcaattctt ctacaatatc
    23161 ccaatgtgct tttggttcaa atgaaaactc acgtggtgta ccccactttt taacttcaac
    23221 gttatcttca tcagattcac cttgaggtac atcatcactt attaaatttg gaatacgaca
    23281 aaggatacct gtcattttat tatcaatttc atttaattga ctatcttttt ctttaatatc
    23341 gtcacctaat gtgcgcattt cagcaatcac atcatcagca ttttctttat tacgtttttt
    23401 taatgcgatt tcttcgctta ctttattacg acgtgctttc atttcttctg ttgcactaat
    23461 taatttacgt cgttgctcat ccaattctaa aatttcatct acaacttttg gatcatctcc
    23521 acgtaattca attttgctct taactgtgtc aggctcattt ctgaataatc taatgtctaa
    23581 cattaacctt catcctttcc caaataatta tcatttatta tggaatgacg tacgtcttta
    23641 ttttttagaa aataaaaaaa gaccacatcc ctacaaggga cgtggtctac gcgttgccac
    23701 cctatttaac aatttaagtt ataaagatac actaaaccta aattgcactt cactaaaata
    23761 acggttatca ccgattgttc ttttaaatta agtaggtaga ttcatatata tgttgattct
    23821 tgttcacact aaccacaagc tctctgatat cgaacactat atattacttg tcctacgaac
    23881 aatgtcttat taagttaatt ttaatatagc aaactatatt tgctttttca agtaacgatt
    23941 tcaaacatca ctcatgtcga tttagtgaca tgcagtcgtt tgataaattg attgctttaa
    24001 atactgtgca accgcttcaa tatctttatg aaattgacgg tcatgtgtaa tggatggcac
    24061 gatacttcga aactcctcat atttacgacg tgtttttggt gataatcctt cgacaccttt
    24121 caactctgct gcttgtaatg caataacaca ttcgattgcc aacacacgtc ttgcattttc
    24181 aataatttga taaccatgtc tagcagctgt agttcccata gatacgtgat cttcttggtt
    24241 cgcagatgaa gtgatagaat caacactcgc tggatgcgct aaagttttat tttcagaaac
    24301 gagacttgca gcagcatatt gcataatcat cgcgccactt tgcaatcctg gctctggact
    24361 aagaaatgct ggtaaatcac catttaattg aggatttact agtcgctcta gacgacgttc
    24421 cgatacgttt gctaattcac ttacacctaa tttaagatga tctaatgcaa aagcaatagg
    24481 ttgtccatgg aagttaccac ctgaaataac aaacgtttca tttgcttcct caaatataag
    24541 tggattatca ttagccgcat tcatttcaaa ttctaattgc tgtttaacat aattgaatac
    24601 ttgaaaactc gcgccatgga tttgtggtat acaacgcaac gtatatgcat cttgtacacg
    24661 tatttctgct tgtcgcgtcg ttaatgttga tccttctaac caatcacgca tacgcgctgc
    24721 cacattaatc tgttcttgaa aattacgaac agagtgcaca tcatgtcgat atgcatctat
    24781 aatgccatta agagactgat gcgttaatgc agcaatccat tcagattggt aacctaaatc
    24841 ttctgcttct atataactaa tgacaccttg agctgtcata gcttgcgtac cattaatcaa
    24901 tgctaaaccc tctttagcct gaaggttcaa aggttgtcta tttaattctc ttaatacatc
    24961 gtcactatcc ttttcttccc ctctgtacaa tactttccct tcaccaatta atgctaatgc
    25021 taaatgtgat aatggcgcta aatctcctga tgcaccgaga gagccttgtt gtgggattat
    25081 cggtataata cgttcattta taaaaaattg taattgtctc actaattcta aagtggcacc
    25141 tgaatgacct tttaataatg tattcaatcg taaaatcatc atgactaatg ctacttcttt
    25201 tgaaaatggc tcacctagtc cacaggcatg tgagcgtatc agattcactt gtaattcatt
    25261 atattgcgtc gggtctatac gtacatcact aaataaccca aaacctgtag tgataccgta
    25321 aaccgtttcc tcattttcaa taatacgttc aactaccgct ctactttttt tgacacgttc
    25381 taacgcatca tcaataattt caatctttga ttgttgttgt aaaaatgatt taatatcctc
    25441 aattgttagt gtttcaccat ctaaatataa agtcatatat gttaccccct tgtttatatt
    25501 aagtaaccca tccttcttga agtatacgtt ttcattttta ttgaaacaat ggttttacgt
    25561 acatttataa cctattatca gagcactatt gtagtgcgtt aaaggatatt aagattgttg
    25621 taagcatatt taataattta tctattgacg aattgcatat acaggtatag tattttctat
    25681 tgtatttaac gacaaataat aatgaattca gaaatttata atacattttg ttaaaagtta
    25741 ctatatattt ttaaaattga ataaatttgg aaaaggcttt tacatgggag gttatatcac
    25801 tatggaaacg ttaaattcta ttaacattcc taagcgtaaa gaagattcac ataaaggtga
    25861 ttatggcaaa attttattaa ttggtggatc tgctaactta ggtggtgcca ttatgttagc
    25921 ggctcgtgca tgtgtattta gcggtagtgg tttaatcact gtagctacac atccaacaaa
    25981 tcattcagca ttacattctc gttgcccaga agcgatggtt attgatatta atgatacgaa
    26041 aatgttgacg aaaatgattg aaatgactga cagtatacta attggtccag gtcttggcgt
    26101 tgatttcaaa ggaaataatg ccattacatt cctactacaa aatatacaac cgcatcaaaa
    26161 tttaatcgta gacggcgatg cgattacaat ctttagtaaa ctgaaaccgc aattacctac
    26221 atgtcgtgtg atctttactc cacacctcaa agaatgggaa cgattaagtg gtattcctat
    26281 tgaggaacag acatatgagc gtaatcgtga agcagttgat cgtttaggtg caactgttgt
    26341 acttaaaaaa catggtactg aaattttctt taaagatgaa gactttaaat taacaatcgg
    26401 tagcccagca atggcgactg gtggtatggg cgatacactt gctggtatga ttacaagttt
    26461 tgtcggtcaa tttgataact taaaagaagc ggttatgagt gccacatata cacatagttt
    26521 tattggcgaa aaccttgcaa aagatatgta tgtggtacca ccatcaagac ttatcaatga
    26581 aataccttac gcaatgaaac aattagaaag ttaatcatta ctaatcattg aatatagtac
    26641 agcattactt tctagcataa aaataagact cccctacata tagggaagtc ttattttttt
    26701 attattcttc atcttcatct gatgattgtt gtatatcttc ttcaacacga tccatgaaat
    26761 cttgtcttac ttcaatacgt ccatcttcat cattttcttc tgaatcaatc acttcagtat
    26821 gaattgcatt tcctggtgtt tcatcattta caaccgcttc acgttgttgt tcagtaccat
    26881 cttcagatac agttgaagta gattgctcat cttcattcgt ttcatcttct gcatcttctt
    26941 ttactttagc aaccgttgaa acaaattgat catcacctaa gcgaattaag cgaacacctt
    27001 gtgctgcacg accattttga gaaatatctg caacatctag tcgaataatg acacctgcat
    27061 tagtaacaat cattaaatct tcttcaccag ttactgtagt gatacataca acattaccat
    27121 tacgctcagt aatcgtagct gttttaatac ctttaccacc acgatttgat aaacgatagt
    27181 cattaactgg cgtacgttta ccataaccat tttcagtaac tactaatact tcatcaacac
    27241 tgtttgcatg agctacatca agccctacaa cttcgtcacc ttcacgaagt gtaatacctt
    27301 tcacacccgt tgctgtacgg cctaaaggac gtaatgttga ttcagggaat cgaattaatg
    27361 atgcatgtga tgtaccaatc aagatatctt cttgaccact tgttaagcga actgcaatta
    27421 actcatcatc ttctctgaac gaaatcgcaa tcttaccatt tctatttatt cttgagaagt
    27481 tacttaatgc tgaacgttta acgacaccac gtttagttgc aaacactaag aagttgtctt
    27541 cactttcaag gtctttaaca gcaatcattg tactaatgac ttcatcattt tcaagttcaa
    27601 tagcattcac tacaggaata cctttagact gtcttgataa ctcaggcact tcataacctt
    27661 taagtttgta tacacgacct ttgttagtaa agaacaatac atggtcatgt gtacttaaag
    27721 ttaccaattg actgacaaaa tcttcttcca atgtattcat accttgaaca ccacgaccac
    27781 cacggttttg agcacgatat gtagataccg gcaaacgttt aatgtagtta ttatggctta
    27841 gtgtaattac aatttgttct tctggaatga gatcttcatc ttctaaatct tcaaatccac
    27901 ctaattggat ttcagtacga cgatcatcac cgaaacgatc tcgaatttct gttaattcat
    27961 ctctaactaa ttgtagtaat acttcttcat cagctaagat tgtttctaat tcactaatat
    28021 aatttaataa ctcattatat tcagcttcaa ttttgtctct ctctaaacct gttagacgtc
    28081 ttaaacgcat gtctaaaata gcttgagctt gtttttcaga aagtttgaag cgttgttgca
    28141 agctttccat tgcaacttta tctgtatctg actcacgaat cgttgaaata atttcatcga
    28201 tatggtcaag tgcgatacgt aatccttcta aaatgtgggc acgatcttta gctttacgta
    28261 agttgtattg cgtacgtctt ctaacaactg tcttttgatg ctctaaataa tgtaccaacg
    28321 cttcttttaa attaataagc ttcggtctac catttacaag tgcaatcata ttcacaccaa
    28381 atgatgtttg aagaggtgtt tgtttgtata agttatttaa aatgacacta gcatttgcat
    28441 ccttacgcac atcaataacg acacgcacac cagtacgtaa acttgtttca tcacgtaaat
    28501 cagtgatacc gtcaattttc ttgtcacgaa cgagctctgc aattttttca atcatacgag
    28561 ccttattcac ttggaaagga atttcagtga caacaatacg ttgacgtccg cctccacgtt
    28621 cttcaataac tgcacgagaa cgcatttgaa ttgaaccacg acctgtttca tatgcacgtc
    28681 taataccact cttacctaaa ataagtccag cagttgggaa atcaggacct tcaatatcct
    28741 ccattaactc agcaattgaa atatcagggt tcttacttaa gctaagtaca ccattgatta
    28801 attctgttaa gttatgtggt ggaatattcg ttgccatacc taccgctata cctgatgctc
    28861 cattggctaa caagttaggg aatcgagcag gtaagactga cggctctctt tcattaccat
    28921 catagttatc gataaaatct attgtatctt tattaatatc acgtaacagt tcaagtgtga
    28981 ttttagtcat acgcgcttca gtataacgca ttgctgctgc gccatctcca tccattgaac
    29041 caaagttacc ttggccatca acaagcggat aacgataact gaaatcttga gccatacgta
    29101 ccattgcttc ataaatagat gagtcaccat gagggtgata tttacccatt acgtcaccaa
    29161 cgatacgtgc tgatttttta tatgatttat ccggtgtcat accttgttca tttaatccat
    29221 atagtatacg acgatgtact ggttttaaac cgtcacgaac atctggcaat gcacgagcaa
    29281 cgataacact catcgcataa tctaaaaatg attcacgcat ttcactggta atatttcgtt
    29341 catttattct tgattgaggt aattcagcca tcaagagttc ctccttcaaa agttcagttc
    29401 acagcgctta gaagtctaag tttgcataaa ctgcattatc ttctataaat tgtctacggt
    29461 tttctacaac gtcacccatt aacatttcaa atgtttggtc cgcttcaatc gcatcttcaa
    29521 gttttacttg taaaagagcg cggtgctcag ggttcattgt tgtttcccat aattgatctg
    29581 cattcatttc tccaagacct ttgtatcgtg caatagacca ttttggtgtt ggattcaatt
    29641 cagatttaag tttatcaagt tccctatcat tgtatacata atacttttgt ttaccttgtg
    29701 tcagtttata caacggtggc tgtgcaatat acacatagcc tgcttcaatt aacggtctca
    29761 taaatcgata gaagaatgtt aataacaatg ttctaatatg cgctccatcc acatcggcat
    29821 cagtcataat gacgattttg tgatatcttg ctttcgctag atcaaagtcg ccaccgattc
    29881 ctgtaccaaa tgctgtgatc atttgacgaa tttcattgtt attcaaaatt ctatctaatc
    29941 gtgctttttc aacatttaat atcttacctc gtaatggtaa aatcgcctgc gttctagagt
    30001 cacgaccaga ttttgtagac cccccggcag agtccccttc gactaagaaa atctcacatt
    30061 cttcaggact tttactagag caatcggcta atttacctgg aagacttgct acatctaacg
    30121 ctgatttacg acgtgttact tcacgcgctt ttttcgcagc aacacgtgca cgtgccgcca
    30181 taataccttt ttcaaccact gtacgtgcga cttgtggatt ttcatataaa aatcgttcaa
    30241 agtgctctga gaataattta tctacaactt gacgcacttc agaattacct aattttgtct
    30301 tcgtttgacc ttcgaattga ggatcaccat gtttgataga tataattgct gtcatacctt
    30361 cacgtgtatc ttcaccagaa agtctatctt tttcttcttt cataatcttg ctacttaaac
    30421 cataactatt taagacacgc gttaatgcac gtttgaatcc gtcttcatgc gtaccacctt
    30481 catacgtatg aatgttattt gcgtaagtta aaagatttgt ggcatatcct gagttatatt
    30541 gaatcgcaat ttctacttca atatcatctt tagattgatg aatataaatt ggctcatcat
    30601 gaataggttc tttattttcg ttcaataact caacgtacga tttaataccg ccctcatagt
    30661 gataggagtc ttctctaacg ttttcttcat cacgttcatc tcttaatgtg atttgaattc
    30721 ctttgtttaa gaaagcaagc tctctaatac gctgctgtaa tgtttcatag ttgtatacag
    30781 ttgtctctgt gaagatttct ccatctgctt taaaacgaat gacagtacct gtcttatcag
    30841 ttgtgccaac ttcttttaag tcaaattgag gtacaccttt tttatatgct tgatgatata
    30901 tagtctcatt tctgtgtaca tatacttcta agtcttgtga caatgcgttt acaactgatg
    30961 aaccaacacc atgtaaacca ccagatactt tgtatccgcc accgccgaat ttaccaccag
    31021 catgtaaaac agttaaaata acttcgacag ctggacgtcc cattttttct tgaatatcaa
    31081 ctgggatacc acgtccgtta tccgttactt taatccagtt atctttttca ataacaactt
    31141 caattttatt tgcataacca gctaatgctt catcgatact attatcgaca atttcccaca
    31201 ctaaatggtg caaacctctc tctgaagtcg atcctatata catacctggt cttttacgta
    31261 ctgcttctaa accttctaat acttgtattt gcccagcacc ataattatcc gtgttgttta
    31321 catctgacaa tgcagtcacc atcgctttct gttactttat aatttcacct tgattaatac
    31381 gatacaattt agcgttattc atgatttcat gatcaatacc atctacagat gtcgtagtga
    31441 caaatgtttg tactttatgc tgaatcgtac ttaataaatg cgtttgacgc gaatcatcta
    31501 attcactgag tacatcgtct aataataaga tgggatattc cccaacttcg atattcatta
    31561 actcaatttc agctaattta atggacaaag ccgttgtacg ttgctgtcct tgagaaccat
    31621 atgtttgagc atccatgcca ttcacatcaa aacttatatc atctcgatgt ggtccgaata
    31681 agctaatgcc tcgttctttt tctctttgca tattatcgct aagaatagac ataatttctt
    31741 caagtcgtgc cgcttcattt tgagcataat caaatttaag actaggtaaa taatttagcg
    31801 acaacgcttc tttatcattt gtgataccag catgaatcgg tttagctaac gactctagct
    31861 cttgaataaa atgtgcacgt ttatcagtta ctttcattgc atattcagca aactgctgat
    31921 ttaatacttc caacattgtt aagtcctttt tttggcctaa ttgtaactgc tttaagtaat
    31981 tattcttttg ctttaaaata cgttggtatt gagctaaatc atttaagtaa acagcagaaa
    32041 tttggcccaa ctccatatct ataaagcgtc gtcttatttg aggagagcct tttacaatat
    32101 tcaaatcttc tggcgcaaat agaaccacat tgaggtgtcc aatatattga gttagacgac
    32161 tttgctctaa gtgattcact ttgacttgtt tacctttttt agttataaac attgttaatg
    32221 gcatcgtgcc gtgtctataa ctaagctcac cttctatttt agcataatca gcattaaaac
    32281 gtatgagttc cttatcatta ctcgttctat gactttttgc taaagctaag gtataaattg
    32341 attcaagtaa atttgtcttt ccttgtgcat tttctccaat gaggatattc acgtcaggat
    32401 gacatttcaa cgtaacctca tcatagttac gataattttc taattggagt gtatttaact
    32461 tcattgttca ccttgatgaa tgattaagaa agaaccagca tcttcaggta attctgggat
    32521 atctatacga tcttgatgtt ctaacttttt accgcgacgt gtttcacgca ctccattaat
    32581 taatacttca acgtcttgca agaaccattt tgcttgacca ccagattcaa taatcccttc
    32641 tgtttttaga aattgaccta aattaatgtc tccttctact acaacttctt gaaccaaaat
    32701 aatcactcca tttctttgtg tgtcattaat atattatacc tttttttgtc acaaatttca
    32761 tttaaaatag agg
//
LOCUS       DAHHSC010000009        32185 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.009, whole genome shotgun sequence.
ACCESSION   DAHHSC010000009 DAHHSC010000000
VERSION     DAHHSC010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 32185)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32185)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32185
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.009"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            complement(125..820)
                     /locus_tag="NBI57_000327"
     CDS             complement(125..820)
                     /locus_tag="NBI57_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCD1821312.1"
                     /translation="MEGNLLQQLFNYYVTNFGYLWDLFFKHLLMSVYGVLFAALIGIP
                     LGILLARYTKLSGFVITIANIIQTVPVIAMLAILMLVMGLGSETVVLTVFLYALLPII
                     KNTYTGIASVDANIKDAGKGMGMTRNQVLRMIELPLSVSVIIGGIRIALVVAIGVVAV
                     GSFIGAPTLGDIVIRGTNATDGTTFILAGAIPIAIIAIVIDVLLRFLEKRLDPTTRHR
                     KNQSNHRPQSINM"
     gene            complement(820..1761)
                     /locus_tag="NBI57_000328"
     CDS             complement(820..1761)
                     /locus_tag="NBI57_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant ABC transporter substrate-binding
                     protein"
                     /protein_id="HCD1821313.1"
                     /translation="MKKIKYILVVFVLSLTVLSGCSLPGLGSKSTKNDVKITALSTSE
                     SQIISHMLRLLIEHDTHGKIKPTLVNNLGSSTIQHNALINGDANISGVRYNGTDLTGA
                     LKEAPIKDPKKAMIATQQGFKKKFDQTFFDSYGFANTYAFMVTKETAKKYHLETVSDL
                     AKHSKDLRLGMDSSWMNRKGDGYEGFKKEYGFDFGTVRPMQIGLVYDALNTEKLDVAL
                     GYSTDGRIAAYDLKVLKDDKQFFPPYAASAVATNELLRQHPELKTTINKLTGKISTSE
                     MQRLNYEADGKGKEPAVVAEEFLKKHHYFDKQKGGHK"
     gene            complement(1778..2413)
                     /locus_tag="NBI57_000329"
     CDS             complement(1778..2413)
                     /locus_tag="NBI57_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCD1821314.1"
                     /translation="MIEFLHEHGGQLMSKTLEHFYISIVALLLAIIVAVPIGILLSKT
                     KRTANIVLTVAGVLQTIPTLAVLAIMIPIFGVGKTPAIVALFIYVLLPILNNTVLGVQ
                     NIDSNIKEAGKSMGMTQFQLMKDVELPLALPLIIGGIRLSSVYVISWATLASYVGAGG
                     LGDFIFNGLNLYDPLMIVTATVLVTALALGVDALLALVEKWVVPKGLKVSG"
     gene            complement(2410..3636)
                     /locus_tag="NBI57_000330"
     CDS             complement(2410..3636)
                     /locus_tag="NBI57_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCD1821315.1"
                     /translation="MLSIKHLTKIYSGNKKAVDDISLDIQSGEFIAFIGTSGSGKTTA
                     LRMINRMIEATEGQIEIDGKDVRSMNPVELRRNIGYVIQQIGLMPHMTIKENIVLVPK
                     LLKWTKEEKDKRAKELIKLVDLPESFLERYPAELSGGQQQRIGVVRALAAEQDIILMD
                     EPFGALDPITRDTLQDLVKTLQRKLGKTFIFVTHDMDEAIKLADKICIMSEGKVVQFD
                     TPDNILRHPANDFVRDFIGQNRLIQDRPNDKTVEGVMIKPITIQADATLNDAVHIMRQ
                     KRVDTIFVVDSNNHLLGFLDIEDINQGIRGHKSLRDTMQQHIYTVQIDSKLQDSVRTI
                     LKRNVRNVPVVDDKQRLVGLITRANVVDIVYDTIWGDSEDAVQTEHVGEDTVSSKMHE
                     QHTTNVKVRDIGDDKS"
     gene            complement(3963..4082)
                     /locus_tag="NBI57_000331"
     CDS             complement(3963..4082)
                     /locus_tag="NBI57_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1821316.1"
                     /translation="MGGCPSVTMDACALLQKFDFCNNISHFRHFFAIKQPIER"
     gene            complement(4162..4302)
                     /locus_tag="NBI57_000332"
     CDS             complement(4162..4302)
                     /locus_tag="NBI57_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1821317.1"
                     /translation="MSKAYRLYNNIQDDTKQPMAHAVGCFFRCVCHGQHFDVGIPLQA
                     WE"
     gene            complement(4306..4905)
                     /locus_tag="NBI57_000333"
     CDS             complement(4306..4905)
                     /locus_tag="NBI57_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI family protein"
                     /protein_id="HCD1821318.1"
                     /translation="MSEKQSNEQVEAFLNKESQWQEEYKYLRALIFNETELEEAYKWM
                     HPCYTLNNKNVVLIHGFKNYVALLFHKGAILEDKYHTLIQQTEKVQAARQLRFENLTE
                     IQARTEEIKYYLAEAIKAEKAGKKVEMKKTEEYVIPKELEAKFEEMPQLESSFYKLTP
                     GRQHQYIYHIGQAKRSETRQKRVEKYINQILEGKGMHDK"
     gene            5085..6722
                     /locus_tag="NBI57_000334"
     CDS             5085..6722
                     /locus_tag="NBI57_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HCD1821319.1"
                     /translation="MNKDKQLHNDKINLSQLVLLGLGSLIGSGWLFGAWEASSIAGPV
                     AIISWVLGFLVIGTIAYNYIEIGTMFPQSGGMSNYAQYTHGSLLGFIAAWANWVSLVT
                     IIPIEAVSAVQYMSSWPWHWAKPMRYLMENGSISTYGLLAVYLIIVIFSLLNYWSVKL
                     LTSFTSLISVFKLGVPMLTIIMLMLSGFDTSNYGHSASTFMPYGSAPIFAATTASGII
                     FSFNSFQTIINMGSEIKNPEKNIARGIAISLSISAVLYIILQSTFITSMPQSMLQHSG
                     WNGINFNSPFADLAILLGINWLAILLYIEAFVSPFGTGVSFVAVTGRVLRAMEKNGHI
                     PKFLGKMNEKYHIPRVAIIFNAIISMIMVTLFRDWGTLAAVISTATLVAYLTGPTTVI
                     ALRKMGPTMTRPFRAKILKVMAPLSFVLASLAIYWAMCPTTAEVILIIILGLPIYFFY
                     EYRMNWRNTKKQIGGSLWIIVYLIVLSILSFIGSKEFKGLNMIHYPFDFIVIIVVALI
                     FYYIGTTSSFESVYFRRATRINTKMRESLNNESKSSH"
     gene            7031..8383
                     /locus_tag="NBI57_000335"
     CDS             7031..8383
                     /locus_tag="NBI57_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylesterase/lipase family protein"
                     /protein_id="HCD1821320.1"
                     /translation="MKINTTGGQIHGITQDGLDIFLGIPYAEPPVHDNRFKHSTLKTQ
                     WSEPIDATEIQPIPPQPDNKLEDFFSSQSTTFTEHEDCLYLNIWKQHNDQTKKPVIIY
                     FYGGSFENGHGTAELYQPAHLVQNNDIIVITCNYRLGALGYLDWSYFNKDFHSNNGLS
                     DQINVIKWVHQFIESFGGDANNITLMGQSAGSMSILTLLKIPDIEPYFHKVVLLSGAL
                     RLDTLKSARNKAQYFQKMMRDYLDTDDVTSLSTDDILMLMAKLKQSRGPSKGLDLIYA
                     PIKTDYIQNDYPTTKPIFACYTKDEGDIYITSEQKKLSPQRFIDIMELNDIPLKYEDV
                     QTAKQQSLAITHCYFKQPMKQFLQHLNIQDSNAQLWLAEFAWHDTSSAHYRSAYHILD
                     MVFWFGNLQILAAHQYPTTAHLKFLSRQMQNDLANFAKSGKMPWPMYHNERRYYRTYQ
                     "
     gene            complement(8445..9632)
                     /locus_tag="NBI57_000336"
     CDS             complement(8445..9632)
                     /locus_tag="NBI57_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000026193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCD1821321.1"
                     /translation="MSIMRLFTFILSIFIVGMVEMMVAGIMNLMSQDLHVSEAVVGQL
                     VTMYALTFAICGPILVKLTNRFSSRPVLLWTLLIFIIGNGIIAVAPNFSILVVGRIIS
                     SAAAALIIVKVLAITAMLSAPKNRGKMIGLVYTGFSGANVFGVPIGTVIGDLVGWRYT
                     FLFLIIVSIIVGFLMMIYLPKDQEIQRGPVNHEAPSHENHVTSKILRPAEVAKYLIIT
                     FLVLIANSVTFVFINPLILSNGHDMSFVSLALLVNGIAGVIGTSLGGIFSDKITSKRW
                     LMISVSIFIVMMLLMNLILPGSGLLLAGLFIWNIMQWSTNPAVQSGVIQHVEGDTSQV
                     MSWNMSSLNAGIGVGGIIGGLVMTHVSVQAITYTSAIIGALGLIVVFTLKNNHYAKIF
                     KSS"
     gene            complement(10028..10804)
                     /gene="fetB"
                     /locus_tag="NBI57_000337"
     CDS             complement(10028..10804)
                     /gene="fetB"
                     /locus_tag="NBI57_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron export ABC transporter permease subunit
                     FetB"
                     /protein_id="HCD1821322.1"
                     /translation="MSNTALGLTALLLVIPIIISYKEGLHIIKDLIVATLRAVVQLII
                     LGFLLHYIFKINDKWLLILCVLVIIINASWNTISRASPVMHHVFWISFLAIFIGTALP
                     LAGTVATGAIHFTANEVIPIGGMLANNGLIAINLAYQNLDRAFVQDGTNIESKLSLAA
                     TPKLASKGAIRESIRLAIVPTIDSVKTYGLVSIPGMMTGLIIGGVPPLQAIKFQLLVV
                     FIHTTATIMSALIATYLSYGQFFNARHQLVARNTDVKSES"
     gene            complement(10797..11459)
                     /locus_tag="NBI57_000338"
     CDS             complement(10797..11459)
                     /locus_tag="NBI57_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCD1821323.1"
                     /translation="MLLEIKDLVYKASDRIILDHISLKVDKGESIAIIGPSGSGKSTF
                     QKQICNLISPTSGELYFKGKPYNDYDPEELRQRISYLMQQSDLFGETIEDNMIFPSLA
                     RNDKFDRKRAKQLIKDVGLGHYQLSSEVENMSGGERQRIAIARQLMYTPDILLLDEST
                     SALDVNNKEKIENIIFKLVEQDVAIMWITHSDDQSMRHFQKRITIVDGKISKVEELNQ
                     HE"
     gene            complement(11707..12783)
                     /locus_tag="NBI57_000339"
     CDS             complement(11707..12783)
                     /locus_tag="NBI57_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HCD1821324.1"
                     /translation="MNQSVKLLKHLTDVNGIAGYEMQVKEAMRNYIEPVSDQIIEDNL
                     GGIFGKKNAENGQYSIMISGHMDEVGFMVTKIDKHGFISFTPVGGWWNQVMLSQKVTI
                     TTDSGKEIRGIIGSKPPHVLTPEERKKPMEIKNMFIDIGVSSKEEAEEAGVEVGNMVT
                     PYSEFEVLANDKYLTAKAFDNRYGCALAVEVLKRLKDENIGINLYSGATVQEEVGLRG
                     AKVAANTIKPDLAIAVDVGIAYDTPGMSGQTSDSKLGGGPVVIMMDATSIAHQGLRKH
                     IKDVAKEHNIEVQWDTTPGGGTDAGSIHVANEGIPTMTIGVTLRYMHSNVSVLNVDDY
                     ENSIRLVTEIVRSLNDESYKNIMW"
     gene            complement(13138..13404)
                     /locus_tag="NBI57_000340"
     CDS             complement(13138..13404)
                     /locus_tag="NBI57_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HCD1821325.1"
                     /translation="MSNYTVKIKNSAKSDLRKIKHSYLKKSFLEIVETLKNDPYKITQ
                     SFEKLEPKYLERYSRRINHQHRVVYTVDDRNKEVLILSAWSHYD"
     gene            complement(13404..13661)
                     /locus_tag="NBI57_000341"
     CDS             complement(13404..13661)
                     /locus_tag="NBI57_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HCD1821326.1"
                     /translation="MIITSPTEARKDFYQLLKNVNNNHEPIYISGNNAENNAVIIGLE
                     DWKSIQETIYLESTGTMDKVREREKDNSGTTNIDDIDWDNL"
     gene            14020..14475
                     /locus_tag="NBI57_000342"
     CDS             14020..14475
                     /locus_tag="NBI57_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1307 domain-containing protein"
                     /protein_id="HCD1821327.1"
                     /translation="MKKLCSLIVVALICIIALSACGKEQTKTYEGDVSGKHVLTSITY
                     KDDKVLKQSTINTIKYDDLGMDKDEAKKLFAKSESIFKDLKGVKYKVDYKDKKAIEHL
                     DIDYTEVDMKKLNKRLGVSTKENKDISFEKLEKQLKHRGLKEKDKMDDK"
     gene            complement(14643..16220)
                     /locus_tag="NBI57_000343"
     CDS             complement(14643..16220)
                     /locus_tag="NBI57_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding glutamate synthase family protein"
                     /protein_id="HCD1821328.1"
                     /translation="MTFLTVMQFIVNIIVVGFLLTVIVIGLIWLIKDKRQSQHSVLRN
                     YPLLARIRYISEKMGPELRQYLFSGDNEGKPFSRNDYKNIVLAGKYNSRMTSFGTTKD
                     YQDGFYIQNTMFPMQRNEISVDNTTLLSTFIYKIANERLFSREEYRVPTKIDPYYLSD
                     DHAIKLGEHLKHPFILKRIVGQSGMSYGALGKNAITALSKGLAKAGTWMNTGEGGLSE
                     YHLKGNGDIIFQIGPGLFGVRDKEGNFSEGLFKEVAQLSNVRAFELKLAQGAKTRGGH
                     MEAEKVNEEIAKIRNVEPYKTINSPNRYEFIHNAEDLIRFVDQLQQLGQKPVGFKIVV
                     SKVSEIETLVRTMVELDKYPSFITIDGGEGGTGATFQELQDGVGLPLFTALPIVSGML
                     EKYGIRDKVKLAASGKLVTPDKIAIALGLGADFVNIARGMMISVGCIMSQQCHMNTCP
                     VGVATTDAKKEKALIVGEKQYRVTNYVTSLHEGLFNIAAAVGVSSPTEITADHIVYRK
                     VDGELQTIHDYKLKLIS"
     gene            16415..17164
                     /locus_tag="NBI57_000344"
     CDS             16415..17164
                     /locus_tag="NBI57_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HCD1821329.1"
                     /translation="MSNTNKHYIEEEYATEQSRFFKRDIGFIFLYIFLVNILPIIIVF
                     LTFGLVAVFTQQAPWGFSFPISMNISYLLAVIISLAIFRRMHEDAFVPMIRTQIIAST
                     KYKWHILISFTLSVVLFLLLNYFDIFALNVDAFTVFDIYPLIRDYDSISLIVMLWLTI
                     ILNTLICQLIFRHILIQELSRLIPLKITIVLSVILETLYYYPYINTWWEFIPALILAS
                     SATYLYLKSNRNFMVSYFYQLAVILVLHIFM"
     gene            complement(17526..18719)
                     /locus_tag="NBI57_000345"
     CDS             complement(17526..18719)
                     /locus_tag="NBI57_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCD1821330.1"
                     /translation="MKGAMAWPFLRLYILTLMFFSANAILNVFIPLRGHDLGATNTVI
                     GIVMGAYMLTAMVFRPWAGQIIARVGPIKVLRIILIINAIALIIYGFTGLEGYFVARV
                     MQGVCTAFFSMSLQLGIIDALPEEHRSEGVSLYSLFSTIPNLIGPLVAVGIWNANNIS
                     LFAIVIIFIALTTTFFGYRVTFAEQEPDTSDKIEKMPFNAVTVFAQFFKNKELLNSGI
                     IMIVASIVFGAVSTFVPLYTVSLGFANAGIFLTIQAIAVVAARFYLRKYIPSDGMWHP
                     KYMVSVLSLLVIASFVVAFGPQVGAIIFYGSAILIGMTQAMVYPTLTSYLSFVLPKVG
                     RNMLLGLFIACADLGISLGGALMGPISDLVGFKWMYLICGMLVIVIMIMSFLKKPTPR
                     PASSL"
     gene            complement(18731..19480)
                     /locus_tag="NBI57_000346"
     CDS             complement(18731..19480)
                     /locus_tag="NBI57_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCD1821331.1"
                     /translation="MIKVTDVEKSYQSAHVFKRRRTPIVKGVSFECPIGATIAIIGES
                     GSGKSTLSRMILGIEKPDKGCVTLNDLPMHKKKVRRHQIGAVFQDYTSSLHPFQTVRE
                     ILFEVMCQCDGQPKEVMEVQAITLLEEVGLSKAYMDKYPNMLSGGEAQRVAIARAICI
                     NPKYILFDEAISSLDMSIQTQILDLLIHLRETRQLSYIFITHDIQAATYLCDQLIIFK
                     NGKIEEQIPTSALHKSDNAYTRELIEKQLSF"
     gene            complement(19473..20288)
                     /locus_tag="NBI57_000347"
     CDS             complement(19473..20288)
                     /locus_tag="NBI57_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCD1821332.1"
                     /translation="MTLLTVKHLTITDTWTDQPLVSDVNFTLTKGETLGVIGESGSGK
                     SITCKSIIGLNPERLGVTGEIIFDGTSMLSLSESQRKKYRGKDIAMVMQQGSRAFDPS
                     TTVGKQMFETMKVHTSMSTQEIEKTLIEYMDYLSLKDPKRILKSYPYMLSGGMLQRLM
                     IALALALKPKLIIADEPTTALDTITQYDVLEAFIDIKKHFDCAMIFISHDLTVINKIA
                     DRVVVMKNGQLIEHGTRESVLHHPEHVYTKYLLSTKKKINDHFKHVMRGDVHD"
     gene            complement(20285..21154)
                     /locus_tag="NBI57_000348"
     CDS             complement(20285..21154)
                     /locus_tag="NBI57_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCD1821333.1"
                     /translation="MIILKRLLQDKGAVIALGIIVLYVFLGLAAPLVIFYDPNHIDTA
                     NKFAGISFQHLLGTDHLGRDILTRLIYAIRPSLLYVFVALFVSVLIGSILGFLSGYFQ
                     GFVDALIMRACDVMLAFPSYVVTLALIALFGMGAENIIMAFILTRWAWFCRVIRTSVM
                     QYTASDHVRFAKTIGMNDMKIIHKHIMPLTLADIAIISSSSMCSMILQISGFSFLGLG
                     VKAPTAEWGMMLNEARKVMFTHPEMMFAPGIAIVIIVMAFNFLSDALQIAIDPRISSK
                     DKLRSVKKGVVQS"
     gene            complement(21151..22086)
                     /locus_tag="NBI57_000349"
     CDS             complement(21151..22086)
                     /locus_tag="NBI57_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCD1821334.1"
                     /translation="MFKFILKRIALMFPLVIVVSFMTFLLTYITNENPAVTILHAQGT
                     PNVTPELIAETNEKYGFNDPLLIQYKNWLLEAMQFNFGTSYITGDPVAERIGPAFMNT
                     LKLTIISSVMVMITSIILGVVSALKRGKFTDRAIRSVAFFLTALPSYWIASILIIYVS
                     VKLNILPTSGLTGPESYILPVIVITIAYAGIYFRNVRRSMVEQLNEDYVLYLRASGVK
                     SITLMLHVLRNAIQVAVSIFCMSIPMIMGGLVVIEYIFAWPGLGQLSLKAILEHDFPV
                     IQAYVLIVAVLFIVFNTLADIINALLNPRLREGAR"
     gene            complement(22099..23697)
                     /gene="cntA"
                     /locus_tag="NBI57_000350"
     CDS             complement(22099..23697)
                     /gene="cntA"
                     /locus_tag="NBI57_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphylopine-dependent metal ABC transporter
                     substrate-binding protein CntA"
                     /protein_id="HCD1821335.1"
                     /translation="MRKLTKMSAMLLASGLILTGCGGNKGLEEKKENKQLTYTTVKDI
                     GDMNPHVYGGSMSAESMIYEPLVRNTKDGIKPLLAKKWDVSEDGKTYTFHLRDDVKFH
                     DGTTFDADAVKKNIDAVQQNKKLHSWLKISTLIDNVKVKDKYTVELNLKEAYQPALAE
                     LAMPRPYVFVSPKDFKNGTTKDGVKKFDGTGPFKLGEHKKDESADFNKNDQYWGEKSK
                     LNKVQAKVMPAGETAFLSMKKGETNFAFTDDRGTDSLDKDSLKQLKDTGDYQVKRSQP
                     MNTKMLVVNSGKKDNAVSDKTVRQAIGHMVNRDKIAKEILDGQEKPATQLFAKNVTDI
                     NFDMPTRKYDLKKAESLLDEAGWKKGKDSDVRQKDGKNLEMAMYYDKGSSSQKEQAEY
                     LQAEFKKMGIKLNINGETSDKIAERRTSGDYDLMFNQTWGLLYDPQSTIAAFKAKNGY
                     ESATSGIENKDKIYNSIDDAFKIQNGKERSDAYKNILKQIDDEGIFIPISHGSMTVVA
                     PKDLEKVSFTQSQYELPFNEMQYK"
     gene            complement(23840..25141)
                     /gene="cntM"
                     /locus_tag="NBI57_000351"
     CDS             complement(23840..25141)
                     /gene="cntM"
                     /locus_tag="NBI57_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_096659686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphylopine dehydrogenase CntM"
                     /protein_id="HCD1821336.1"
                     /translation="MSKLLMIGTGPVAIQLANICYLKSDYEIDMVGRASTSEKSKRLY
                     QAYKKEKQFEVKIQNEAHQHLEGKFEINRLYKDVKNVKGEYETVVMACTADAYYDTLQ
                     QLSLETLQSVKHVILISPTFGSQMIVEQFMSKFSQDIEVISFSTYLGDTRIVDKEAPN
                     HVLTTGVKKKLYMGSTHSNSTMCQRISALAEQLKIQLEVVESPLHAETRNSSLYVHPP
                     LFMNDFSLKAIFEGTDVPVYVYKLFPEGPITMTLIREMRLMWKEMMAILQAFRVPSVN
                     LLQFMVKENYPVRPETLDEGDIEHFEILPDILQEYLLYVRYTAILIDPFSQPDENGHY
                     FDFSAVPFKQVYKNEQDVVQIPRMPSEDYYRTAMIQHIGKMLGIKTPMIDQFLTRYEA
                     SCQAYKDMHQDQQLSSQFNTNLFEGDKALVTKFLEINRTLS"
     gene            complement(25134..25952)
                     /gene="cntL"
                     /locus_tag="NBI57_000352"
     CDS             complement(25134..25952)
                     /gene="cntL"
                     /locus_tag="NBI57_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-histidine (S)-2-aminobutanoyltransferase CntL"
                     /protein_id="HCD1821337.1"
                     /translation="MNNFNNEIKLILQQYLEKFEAHYERVLQDDQYIEALETLMDDYS
                     EFILNPIYEQQFNAWRDVEEKAQLIKSLQYITAQCVKQVEVIRARRLLDGQASTTGYF
                     DNIEHCIDEEFGQCSITSNDKLLLVGSGAYPMTLIQVAKETGASVIGIDIDPQAVDLG
                     RRIVNVLAPNEDITITDQKVSELKDIKDVTHIIFSSTIPLKYSILEELYDLTNENVVV
                     AMRFGDGIKAIFNYPSQETAEDKWQCVNKHMRPQQIFDIALYKKAAIKVGITDV"
     gene            complement(25963..26784)
                     /gene="cntK"
                     /locus_tag="NBI57_000353"
     CDS             complement(25963..26784)
                     /gene="cntK"
                     /locus_tag="NBI57_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine racemase CntK"
                     /protein_id="HCD1821338.1"
                     /translation="MNRQVIEFSKYNPSGNMTILVHSKHDASEYASIANQLMAATHVC
                     CEQVGFIESTQNDDGNDFHLVMSGNEFCGNATMSYIHHLQESHLLKDQQFKVKVSGCS
                     DLVQCAIHDCQYYEVQMPQAHRVVPTTINMGNHSWKALEIIYETYVHYVIPVKQVTTE
                     IQHLVEAFVREQQWSHKYKTVGMMLFDEQRQFLQPLIYIPEIQSLIWENSCGSGTASI
                     GVFNNYQRNDACKDFTVHQPGGSILVTSKRCHQLGYQTSIKGQVTTVATGKAYIE"
     gene            complement(27474..28241)
                     /locus_tag="NBI57_000354"
     CDS             complement(27474..28241)
                     /locus_tag="NBI57_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001599343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HCD1821339.1"
                     /translation="MNIRHIRNATAVIEYGGKRILIDPMLSDKGSFDPFPNSPRQDQK
                     NPLVELPMAIEDIINDIDLVILTHLHIDHFDPKAIEVLPKDIKIYAQNEEDASEVERY
                     GFTNVSVFNDVTHIGEIELIKTDAQHGHGEILKMTGHVHGMILKHSEEPTLYLAADTV
                     WFEGVEKALKTYQPDVVVLNGGANQFFEGGPLVMDEHDVLKVANTLPLAQIVVVHMEA
                     VNHWHLSRKELNEFINSNDLGNRVVVPNDGELLTFEK"
     gene            complement(28341..29159)
                     /locus_tag="NBI57_000355"
     CDS             complement(28341..29159)
                     /locus_tag="NBI57_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001620216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCD1821340.1"
                     /translation="MKRLENKVAVVTGASTGIGQASAIALAQEGAYVLAVDIAEAVSE
                     TVDKIKSNGDNAKAYNVDISDEQQVVDFVSDIKEQFGRIDVLFNNAGVDNAAGRIHEY
                     PIDVYDKIMNVDMRGTILMTKMMLPLMMNQGGSIVNTSSFSGQAEDLYRSGYNAAKGA
                     VINFTKSIAIEYGRDGIRSNAIAPGTIETPLVDKLTGTSEDDAGKTFRENQKWMTPLG
                     RLGKPEEVAKLVVFLASDDSSFITGETIRIDGGVMAYTWPGEMLSDDSWKRTLE"
     gene            29394..30932
                     /locus_tag="NBI57_000356"
     CDS             29394..30932
                     /locus_tag="NBI57_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
                     /protein_id="HCD1821341.1"
                     /translation="MTSKHQQKGSIVNRFLNSVEKIGNKLPDPSVLFFLMCVGLAIMT
                     WVISLFNVSVKHPGTHQTIYIKNIISHDGFTMIMNDTIKNFSEFPALGLVLAVMIGIG
                     VAEKTGYFDKLMISVVNRAPRFLILPTIILIGILGSTAGDAATIILPPLAAMLFIKIG
                     YHPIAGLTMAYASAVGGFAANIVVGMQDALVYSFTEPATRIVSDSIKTNVAMNWYFIA
                     ASVVVLLPTILLVTTKLIIPRLGKYDDSLMHDDHEEASSHITDKEAHALKWANISFIV
                     TIILLIITAIPEHSFLRNAKTGSLLDDAPLINGVGLIILVVFLVPGLVYGILSKEIKN
                     TKDLGKMFGDAVGSMGTFIVIVFFAAQLLAYLKWSNLGIIAAVKGAKLLEHQNGIVLI
                     LGIIVLSAMVNMLIGSASAKWGILGPIFVPMLILIGFHPAFTQVIYRVGDSITNPITP
                     MMPYLPLLLTYAQKYDKRMKLGALLSSLMPYSIALSIVWTVFVIIWFLLGIPVGPGGP
                     IFVK"
     gene            complement(31045..31467)
                     /locus_tag="NBI57_000357"
     CDS             complement(31045..31467)
                     /locus_tag="NBI57_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HCD1821342.1"
                     /translation="MPYNYKKQNGELMSVMSQGEKFIHQSPVNDELSALIKLLISKIN
                     GCHYCVDIHKKELKELGVTQMKIDEVLSFRHLDLFTDQEKVTLEFAEMLNSIKDFKKF
                     EIIDRLKSFYDEEQIIDLVFVVNQINGWNRLNIISDRL"
     gene            complement(31919..>32185)
                     /locus_tag="NBI57_000358"
     CDS             complement(31919..>32185)
                     /locus_tag="NBI57_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1433 domain-containing protein"
                     /protein_id="HCD1821343.1"
                     /translation="QHNTKEPNTIKSVHFTSLKRGPMGDAVIEGYINENKKDNFTAYA
                     TPEHNYQFGGAMIESERLSELLKPAHQLKSPDEIKEELDKKEGH"
BASE COUNT        10209 a         6249 c         4771 g        10956 t
ORIGIN      
        1 tagtgtaaac gcatacaaac acaacctata aaaaggctac tcaaagatta tttgagctcc
       61 ttgagtagcc tcatttttta taaatcatga aacgaaatcg ctaaattata aacatcttct
      121 actattacat attaatactt tgcggccgat gattagattg atttttacga tgtcgtgttg
      181 ttgggtctaa tcgtttttct aaaaatctta atagtacatc aatgacgatt gcaatgatag
      241 caatcggaat cgcacctgct aaaataaacg ttgtgccatc cgtcgcattt gtaccacgaa
      301 tcacaatgtc accaagcgta ggtgctccta taaatgatcc aacggcaaca acacctatcg
      361 caacaaccaa ggcaatacga atgccaccga taataaccga aacagataac ggtaattcaa
      421 tcattcgtag cacttgattg cgtgtcattc ccataccttt gccagcatcc ttaatattcg
      481 catcaacact agctatacca gtataagtgt tttttataat tggaagtaac gcatataaaa
      541 acactgttaa aactactgtt tctgaaccta agcccatgac taacattaaa atagctaaca
      601 ttgcaatgac tggaactgtt tgaattatat ttgcaattgt aattacaaat ccagaaagtt
      661 ttgtgtatct tgcaagcaag attcccaatg gaataccaat taaagctgca aacagcacac
      721 catagacaga cattaataag tgtttgaaaa ataaatccca tagataacca aagttcgtaa
      781 cataataatt gaataattgc tgtaataaat taccttccat tacttatgac cacctttctg
      841 tttatcaaaa tagtggtgtt ttttcaaaaa ttcttctgcg acgacagcag gttctttacc
      901 tttaccatcc gcttcataat tcaagcgttg catctctgaa gtcgaaatct ttcctgtcaa
      961 cttattaatc gtcgttttaa gttctgggtg ttgccgtaat aattcatttg ttgcaacagc
     1021 actcgcagca taaggtggga aaaattgttt atcatcttta agtactttca aatcatacgc
     1081 ggcaattcga ccatctgtag aataacctaa tgcaacgtct aacttctctg tgtttaatgc
     1141 gtcgtagact agacctattt gcattggtct cactgtacca aagtcaaaac catactcttt
     1201 tttaaatcct tcatagccat cgcctttacg attcatccat gaactatcca tacctaaacg
     1261 taaatcttta ctatgctttg ctaaatctga aactgtctct aaatgatatt ttttagcagt
     1321 ttccttcgtt accatgaatg catacgtatt cgcaaaacca tacgaatcaa aaaacgtttg
     1381 atcaaatttc tttttaaatc cttgttgtgt ggctatcatt gctttcttag gatctttaat
     1441 tggtgcttcc ttcaaagctc ccgttaaatc tgtgccatta tatctaacac ctgatatatt
     1501 agcatcccca ttaattaagg cattatgttg aatcgtactt gaccctaaat tatttactaa
     1561 tgttggcttt atcttaccat gtgtatcatg ctctattaac aaccgtaaca tatgtgaaat
     1621 aatttgcgat tcgcttgttg ataatgctgt aattttgaca tcatttttcg tgctcttact
     1681 acctagtccg ggcaaactac atccagataa tacggtaagc gataagacaa acacgacaag
     1741 tatatattta attttcttca ttatcttagc ctcctaatta tccagatact tttaagcctt
     1801 tgggaactac ccatttttca actaaagcta ataaggcatc aacacctaat gctagtgcag
     1861 taacgagtac cgttgcagtt acaatcatca gtggatcata taaatttaaa ccattgaaaa
     1921 tgaaatcacc taatccaccc gcacctacat aacttgcaag tgtagcccaa ctaattacat
     1981 acacagatga caaacgaatg ccaccaatga taagcggcaa tgctaacggc aattcaacat
     2041 ccttcatcaa ttgaaattgt gtcattccca tactttttcc agcttcttta atgttgctat
     2101 caatattttg aacaccgagt accgtgttat ttaaaatagg taataataca taaataaata
     2161 gcgctacaat tgcaggcgtt ttaccaacac caaaaatcgg tatcataata gcaagtacag
     2221 ctagtgttgg aatagtttgt aagacacctg ccacagttaa tacaatattg gcagttcgct
     2281 ttgtttttga taataaaatg cctataggta ctgcaacaat gatggcaagt aataatgcca
     2341 ctatagaaat ataaaaatgt tccagtgttt tcgacatcaa ctgtccacca tgttcatgta
     2401 ggaactcaat catgatttat catctcctat gtcacgtact ttgacattag tagtgtgttg
     2461 ctcatgcatt tttgaggaca cagtgtcttc ccccacatgt tctgtttgca ctgcatcctc
     2521 actatcgccc caaatcgtgt catatacaat gtcaacaaca ttggcacgcg taatcagtcc
     2581 tactaaacgt tgcttatcat ctacgacagg tacattccta acgtttcttt ttaaaatcgt
     2641 acgtacagaa tcttgtaatt tagaatcaat ttgaacggta taaatatgtt gttgcatggt
     2701 gtctcgtaaa cttttgtgtc cacgtatacc ctgatttata tcttcaatgt ctaagaaacc
     2761 tagtaaatgg ttattactat ctactacaaa aatagtatca acacgctttt gtctcataat
     2821 atgaacagca tcattaagcg ttgcatctgc ctgtatcgtg attggtttaa tcattacacc
     2881 ttctacagtc ttgtcattgg gacggtcttg aatcagtcta ttttgtccta taaaatcacg
     2941 tacaaaatca tttgcgggat gtcttaaaat attgtctggt gtatcaaatt gcaccacctt
     3001 accttctgac ataatacaaa ttttgtctgc taatttaatc gcttcatcca tatcatgtgt
     3061 tacaaagata aacgtcttgc ctaattttcg ttgtaacgtt ttaactaaat cttgtaacgt
     3121 atctctcgta ataggatcca atgcaccaaa aggttcatcc attaaaataa tatcttgttc
     3181 ggccgcaagt gctcttacga caccgatacg ttgttgttgc ccacctgata gttctgctgg
     3241 ataacgctct aaaaatgact ccggtaaatc cacaagttta attaattcct ttgcacgttt
     3301 atccttttct tctttagtcc atttcaacaa tttgggtacc aatacaatat tctctttaat
     3361 cgtcatatga ggcattaacc caatttgttg aataacataa ccaatatttc tacgtaattc
     3421 gacaggattc atactccgaa catctttacc atcaatttca atttgtcctt ctgtcgcttc
     3481 aatcatacgg tttatcattc ttaaagcagt tgttttacca cttccactgg ttccaataaa
     3541 tgcgataaat tccccagatt gaatatctaa agagatgtca tctactgcct ttttattacc
     3601 agaataaatt ttcgttaaat gcttaatact taacataatc atttctcctt cctcataaaa
     3661 aatatgacga atgatacgca taaaataaaa agaccattat cgcaattgtt aaacaaatag
     3721 agtcataagt taaacaagta cacatcatgg tcttgattat gtagaatttt atatttttca
     3781 tctgtaatgt gaatacttaa cagtaaacgg cgctatgtat gttgaatttc taccgattac
     3841 tttgtttgca cattcgtata ttcgacattt aaaactaaca tactctatat gatgttatta
     3901 aacattatta ttttatcttt ggcaactaaa tagataactc atatttttta aaagcgaatt
     3961 tattatcgct caattggttg ttttatagca aaaaagtgtc gaaaatgtga aatattatta
     4021 caaaagtcga atttttgtaa taatgcacac gcatccattg ttacagacgg acaaccgccc
     4081 atccgccact cacaaagtca tccttatatt ttaatatctt catcattcat tgtagacacc
     4141 cttgcctttt gctaacattt tctactccca agcctgtaac ggaattccaa cgtcaaagtg
     4201 ttgcccatga cagacacacc taaaaaaaca accaacagca tgagccattg gttgtttcgt
     4261 gtcatcttgt atgttgttgt ataaccggta tgctttactc attaattact tatcatgcat
     4321 ccctttacct tctagtattt ggttaatata cttttcaaca cgcttttgtc ttgtttcact
     4381 gcgtttagct tgtccaatat gatatatata ttgatgttgt ctgcctggcg ttaatttata
     4441 aaatgaagac tctaactgtg gcatttcttc gaacttagct tctaactctt taggaataac
     4501 atattcctct gttttcttca tttcaacttt tttaccagct ttttcagctt taattgcttc
     4561 ggctagataa tatttaattt cttcggtacg tgcttgaatc tctgttaaat tttcaaatcg
     4621 taactgacga gctgcttgca ccttttcagt ctgttgaatg agtgtatgat atttatcctc
     4681 caaaatggca cctttatgaa ataatagtgc aacataattt ttgaagccat ggataagtac
     4741 tacattttta ttattcaacg tgtaacaagg atgcatccat ttatacgctt cctctaattc
     4801 tgtttcatta aagattaacg ctctcaaata tttatattct tcttgccatt gactctcttt
     4861 atttaaaaat gcttcaactt gttcatttga ttgtttctca ctcatagtca tcacttccat
     4921 tttgtttttc aatacacatt gtattatata tcctaatttt ataaaaaact aattttacaa
     4981 atgcttttgc gttcttacaa aaaatgcatt tgactattat tataataagc gtataattgt
     5041 cgcatattat tttttgtatt tttggcaata acgaaggagt atttatgaat aaagacaagc
     5101 aattgcacaa cgacaaaatc aatctatccc aattagtctt attagggtta ggctctttaa
     5161 taggatctgg ttggctattt ggtgcgtggg aagcatcatc aatagctgga ccagtagcaa
     5221 tcatatcatg ggttcttgga ttcctagtca ttggaaccat tgcctataac tacattgaaa
     5281 tcggcacaat gtttcctcaa tcaggtggca tgagtaacta tgcccagtat acacatggct
     5341 cattattagg ctttattgct gcttgggcga attgggtgtc tttggtgaca ataataccta
     5401 tcgaagctgt gtcagctgtt caatatatga gttcttggcc gtggcattgg gcgaaaccaa
     5461 tgagatattt aatggaaaat ggctctatta gcacatacgg attgctagct gtatatctca
     5521 tcattgttat tttttcatta ttaaactatt ggtccgtaaa acttttaaca tcatttacga
     5581 gtttaatttc tgtatttaaa ttaggcgtac ccatgttaac catcatcatg ttgatgttat
     5641 caggattcga cacttcaaat tacggccatt cggcaagcac atttatgcct tacggaagtg
     5701 caccgatttt tgctgcaaca acagcatcag ggattatttt ttcattcaat tcattccaga
     5761 caattattaa tatgggttca gaaattaaaa atcctgaaaa aaatatcgca agaggcatcg
     5821 ctatttcact gtcaatcagt gcagtgttgt acatcatttt acaaagtacg tttatcactt
     5881 ctatgcctca atcaatgtta caacatagtg gatggaatgg catcaacttc aattcaccat
     5941 ttgcagattt agctatctta ttaggaatta attggctcgc aattttacta tatattgaag
     6001 cttttgtatc accattcggt actggcgtgt catttgtcgc cgttacaggt cgagttttac
     6061 gagcaatgga gaaaaatgga catatcccta aatttcttgg gaagatgaat gaaaaatatc
     6121 atatcccacg tgtagcaatc atctttaatg ccatcattag tatgattatg gttacattat
     6181 ttagagattg gggtacgcta gcagcagtta tttctactgc aactttagta gcctatttaa
     6241 ctggcccaac gacagtgatt gcattaagaa aaatgggacc aacaatgact cgtccattta
     6301 gagcaaaaat tttaaaagta atggcaccat tatcatttgt attagcttca ttagctatat
     6361 attgggcaat gtgtccaaca acggctgaag ttattttaat cattatactt ggattaccaa
     6421 tctacttctt ctatgaatac cgtatgaatt ggcgtaatac aaagaaacaa attggtggta
     6481 gcttatggat tattgtatat ttaatcgtgc tatcaatact gtcatttata ggaagcaaag
     6541 aatttaaagg cttaaatatg attcactatc catttgactt tatcgttatt attgttgtgg
     6601 cgcttatatt ctattacatc ggtacaacga gttcatttga aagcgtctat ttccgtcgcg
     6661 caacacgaat caatacaaag atgcgcgagt cactaaataa tgaatcaaag tcatctcatt
     6721 aaatatttta aaatgcaatg ccatatatga tttcaacaca cacacattaa ccaaccattg
     6781 atttcaacat cttggttggt tttttatttt gaaaatcggt tataaataac taacataaca
     6841 atgtggcgag agtgatattg taatgaaatg tcactgtaga agaacatcat ataatgctga
     6901 aaatggtttg agttaaaaca gatatcaagc tagtctgatt cagtcacaaa attgtcttgt
     6961 tatacttgtc acctatcatc tatagaccgt ggtatgatta aattggggat gataaaggag
     7021 gttaataaat atgaagatta atactacagg tggtcaaatt catggtatta cacaagatgg
     7081 tttagatatc ttcttaggca ttccttatgc agaaccacca gttcatgata atcgctttaa
     7141 acattctacg ttaaaaacac aatggtcaga gccaattgat gcaactgaaa tacaacccat
     7201 cccaccgcaa ccagacaaca aattagaaga ttttttctcc tcacaatcta caacttttac
     7261 tgaacatgaa gactgtttat atctaaatat ttggaaacaa cataatgatc agacgaagaa
     7321 acctgtcatc atttattttt atggtggtag ttttgaaaat ggtcatggta cagccgaact
     7381 ctatcaaccg gcacatttag tacaaaataa cgacattatc gttattacat gcaattatcg
     7441 tttaggcgca ttaggatatt tagactggtc atattttaat aaagattttc attccaataa
     7501 tggactttca gatcaaatca atgtcataaa atgggtgcat caatttattg aatcctttgg
     7561 tggcgacgct aataacatta ctttaatggg tcagtctgca ggcagtatga gcattttgac
     7621 tttacttaaa atacctgaca ttgagccata cttccataaa gtcgttctac taagtggcgc
     7681 actacgatta gacaccctta agagtgcacg caataaagca caatatttcc aaaaaatgat
     7741 gcgcgattat ttagatacag atgatgttac atcattatcg acagatgata ttcttatgct
     7801 gatggcgaag ctaaaacaat ctcgaggacc ttctaaaggg cttgatttaa tatatgcgcc
     7861 tattaaaaca gattatatac aaaatgatta tccaacaacg aaaccaattt ttgcatgtta
     7921 tacaaaagat gaaggcgata tttatattac tagtgaacag aaaaaattat cgccgcaacg
     7981 ctttatcgac attatggaat taaatgatat tcctttaaaa tacgaagatg ttcagactgc
     8041 gaagcaacaa tctttagcga ttacacattg ttatttcaaa cagccgatga agcaattttt
     8101 acaacatctt aatatacaag attcaaacgc acaactatgg cttgctgaat ttgcatggca
     8161 tgatacttct agcgcacact atcgcagtgc ttaccatatt ctagatatgg tattttggtt
     8221 cggaaactta caaatattag cagcacatca atatccaaca actgctcacc ttaaattttt
     8281 aagtcgacaa atgcaaaatg atttagctaa ttttgctaaa agcggtaaaa tgccttggcc
     8341 gatgtatcat aatgaacgtc gctattatcg cacatatcaa taacgttatc gcatctaaaa
     8401 aagcacacct gaatttgata gcaggcgtgc ttttcatatg agaattatga tgatttaaat
     8461 attttagcat aatgattatt tttcaatgtg aaaacaacga ttaatcctaa tgcgccaatg
     8521 atggcactcg tatatgtgat agcttgaaca gaaacatgtg tcatgaccaa gcctccaata
     8581 atgccgccaa caccaatacc agcgtttaaa ctagacatgt tccaactcat tacttggctt
     8641 gtgtcgcctt caacatgttg aatcacaccg ctttgcactg ctggattagt actccattgc
     8701 atgatattcc aaataaatag tcctgctaac aatagacctg aaccaggtaa gattaaattc
     8761 ataagtaaca tcataacgat aaaaatagaa acagaaatca ttaaccaacg cttacttgta
     8821 attttatcgg agaatatacc acccaatgat gttccaataa cgccagcgat tccatttact
     8881 agaagtgcta atgaaacgaa tgacatatca tgaccattag ataaaataag tggatttata
     8941 aagacgaatg tcactgagtt tgcaatcaat actaaaaacg taataattaa atattttgct
     9001 acttcagcag gtcttaatat tttcgaagta acatgatttt catgagatgg tgcctcatga
     9061 ttcacagggc ctcgttgtat ttcctgatcc ttcggtaaat agatcatcat caagaagcca
     9121 acaataatac tcacaataat taagaataga aatgtatagc gccaacctac taaatcgccg
     9181 ataaccgttc caattggtac accaaaaaca ttggccccac taaaccctgt atagacaagt
     9241 ccaatcattt tgccacgatt tttaggtgct gataacatcg ctgtaatagc taatactttt
     9301 acgataatta gtgctgctgc tgcagatgag ataattctac caactactaa tattgaaaaa
     9361 tttggcgcta cagcaataat gccattacca atgataaata taagtaatgt ccataataat
     9421 acaggccttg atgaaaaacg gttcgttaat ttaaccagaa taggtccaca tatcgcaaat
     9481 gttaaagcgt acattgtcac taattgacca acgacagctt ctgatacatg taagtcctga
     9541 ctcatcaagt tcataattcc tgcaaccatc atttcaacca ttcctacgat aaaaatactt
     9601 aaaatgaatg taaatagtcg catgattgac attttaaaac tcctttcaaa caaaatttcc
     9661 gatattcatt tcccttagaa taacgattta ttaaaaaatg tgtgcaacac aattttatat
     9721 ataaccgttc tattatataa tattttgctt taattttctg tgcttaaatg gaaaattatt
     9781 gaaattgttt ttcagtattt gaaaaattaa cacaagtatg tcatttaata attatgtata
     9841 attacttcag tttcaacaat gtcttcaaaa aagcacgcac gctttatatg tcagaacaga
     9901 taactgacta caaagtatgc gtgcctttat gaatattttc gctgtgttaa tgaatagcga
     9961 tactatatgt gcgcgtcact tcatttccaa ttaaaactaa tgcctaaatc tgatgcagta
    10021 aaatctatca tgattcactc ttaacatccg tatttcgtgc tactaattga tgtcttgcat
    10081 tgaaaaattg accatagctt aaatatgtcg caatcaaagc agacataatg gtcgcagttg
    10141 tatgaataaa cacgactaac aattgaaatt taatcgcttg taaaggtggt acgccaccaa
    10201 taattaagcc tgtcatcata ccaggaatcg acacaagccc atatgtttta accgaatcaa
    10261 ttgttggcac tatagctaaa cgaatacttt cacgtattgc acctttagaa gccaatttag
    10321 gtgtagctgc aagtgataat ttagattcaa tattagtacc atcttgtacg aatgcacgat
    10381 ctaaattctg gtaagctaaa ttaattgcaa tcaagccatt atttgcaagc atgccgccga
    10441 taggtataac ttcattggct gtaaaatgaa ttgcccctgt agctacagta cctgcaagtg
    10501 gtaatgctgt tccaatgaag atagctagaa atgatatcca aaacacatga tgcatcactg
    10561 gtgatgctcg actaattgta ttccatgatg cattaataat aatgaccaat acacataaaa
    10621 taagcagcca tttatcgttt attttaaaaa tataatgcag caaaaatccc aaaatgatta
    10681 attgcaccac tgcgcgtagt gtcgcaacaa ttaaatcttt aataatatgt aaaccttctt
    10741 tatatgaaat gataatcgga atgactaaaa gcaatgccgt caatccaagt gccgtattac
    10801 tcatgttgat tcaactcctc aaccttagaa attttgccat caacaatcgt tatgcgcttt
    10861 tgaaaatgtc tcatactttg atcatcacta tgggtaatcc acatgatagc aacatcctgt
    10921 tccactaact taaaaatgat attttctatc ttttctttat tattaacgtc taatgcactg
    10981 gtcgattcat ctaataaaag aatatccggt gtatacatca gttggcgcgc tatagcaatt
    11041 ctttgccgct caccacccga catattttcc acttccgaac ttaattgata atgtcccaaa
    11101 ccgacatctt taattaattg ctttgcacgt tttctatcaa atttatcatt acgtgcaagt
    11161 gatgggaata tcatgttatc ttcaatcgtt tcaccaaaca agtcactttg ctgcatcaaa
    11221 taactgattc gttgacgcaa ttcttccggg tcataatcat tatagggttt acctttaaaa
    11281 taaagttctc cactagttgg actaatcaaa ttacatattt gcttttgaaa tgtactttta
    11341 ccactacctg atggacctat aatggcaata ctctcgcctt tatctacttt tagactgata
    11401 tgatctagta tgattctatc gctcgcttta tacactaaat ctttaatttc taacaacatt
    11461 tgcaccgctc ctcatgtaat atatcaggta tattattccc gtttttttgg tttttagtgc
    11521 aattacattt aagaaaaaat cattttcttg taatatcaaa atttaggcat aattgatttt
    11581 cagcatttaa attaaaaaca aaaaatccta ttaacatgga tactgtctaa tgactaagaa
    11641 ttaaagttag acaattgttt aacaacctac catattaaaa ggattcttta ttatttatgg
    11701 atttgattac cacatgatat ttttataact ttcatcattc aatgaacgga caatttcagt
    11761 aacaagacgg atagaatttt cataatcatc tacattgagc actgaaacat tagaatgcat
    11821 gtatcgcagc gtaacaccga ttgtcatcgt tggaatacct tcatttgcga catgaatact
    11881 tcccgcatct gtacctccac ctggtgtcgt atcccattgt acttcgatgt tatgttcctt
    11941 agctacatct ttaatatgct tacgcaaacc ttggtgagca atacttgtag catccatcat
    12001 aatgacaact ggaccaccgc ctagtttact atcgctcgtt tgacctgaca tacctggggt
    12061 atcataagca atacctacat cgacagctat cgccaaatct ggtttaattg tattcgctgc
    12121 cactttcgca ccacgcaaac caacttcttc ttgcactgtg gcaccactgt ataagttaat
    12181 tccaatattt tcatctttta aacgttttaa tacctcaaca gctaatgcac agccatagcg
    12241 attatcaaat gctttcgcag ttaaatattt atcatttgca agcacttcaa attcactata
    12301 tggcgttacc atattgccta cttcaacgcc agcttcttca gcttcttcct tgctactaac
    12361 accaatatct ataaacatat ttttgatttc cattggcttt ttacgttctt caggcgttaa
    12421 gacatgtggc ggtttagaac cgatgatacc tctaatttct ttgcccgaat ctgttgtaat
    12481 cgttactttt tgagatagca tgacttgatt ccaccatcca ccaactggcg taaatgaaat
    12541 aaaaccatgt ttatcaatct ttgttaccat aaaaccaact tcatccatat gaccagaaat
    12601 cataattgag tattgaccat tctcagcatt tttctttcca aaaatgccac ccaagttatc
    12661 ttcaataatt tgatcactga caggctctat atagttacgc attgcttctt taacttgcat
    12721 ttcataacca gcaatgccgt ttacatctgt taaatgttta agtaatttga ctgactgatt
    12781 catgttattc atcctcctgt atttcaactt aaaactattg tatcactaac tttcagaaaa
    12841 acgttcccta ccgctttatc attttcaggc ttaaattttt taaaattttg tgagaaatcc
    12901 gcttcaaaaa cctcatattt ttaaaattta atattccgaa aaagtcacaa tttaaaattc
    12961 cttattataa tatgttattc ttttattgtt actaatttca atttgtttag tcttgcgcta
    13021 ttataaaatt aaaaagtgta gagataacaa cgcattaacg tcgctatttt ccctacactt
    13081 aaatactagt ttctttaatt aacttaccat taaagttatt cagatattga atattcatta
    13141 atcataatgt gaccatgccg atagtattaa tacttcttta tttcgatcat ctacggtata
    13201 gacgaccctg tgctgatggt taattcttct tgaatatcgc tctaaatatt taggctctaa
    13261 tttttcaaaa gattgtgtta ttttatacgg atcatttttt aaagtctcaa caatttctaa
    13321 aaatgacttc tttaaataag aatgttttat tttccttaaa tctgatttcg ctgaattttt
    13381 aatcttaacc gtgtaattgc tcattaaaga ttatcccaat caatatcatc tatatttgtt
    13441 gtaccactat tatctttttc tctttctctt actttgtcca ttgtaccagt agattcaaga
    13501 tatattgtct cttgtatact tttccaatct tctaaaccta taatcacagc attattttcg
    13561 gcattattgc cactaatata aattggttcg tgattattat taacattttt tagtaattga
    13621 taaaaatctt ttctcgcttc tgtagggcta gtgataatca tataaaccct cctttgtttt
    13681 ctgttacgct aattatagcg tacgcacttg agtacgtcaa tattgccaac gagttcatcg
    13741 tattcttaat acctatatgc tatcgtacac aagcttttgt tcttttcttc caacataccg
    13801 tctataatgc tcaaaaagaa atcagcagtg gaacacacta cgttccctac cgctttatca
    13861 tttttcaggc ttaaatattt ttaaaatttt gtgaggaatc cgcttaaaaa actcatatct
    13921 tcaaaaaaaa tgacaaaatt taaatttttg aataacatca ttttttagta aatgttataa
    13981 tttctttata atacaaatat cgaaagaagg cgactgactt tgaaaaaatt atgttcatta
    14041 attgtagtag cattaatttg tatcattgca ttatcagctt gtggtaaaga acaaaccaaa
    14101 acatatgaag gtgatgttag tggtaaacat gtactaactt ctatcacata taaagatgat
    14161 aaggttttaa aacagtctac aatcaacaca attaaatatg acgatctagg tatggataaa
    14221 gacgaagcca aaaagttatt cgccaaatct gaaagtattt tcaaagacct taaaggcgta
    14281 aaatacaaag tagactataa agataaaaaa gcaattgaac acttagacat agattacaca
    14341 gaagttgaca tgaaaaaatt aaataaacgt cttggtgttt caactaaaga aaataaagat
    14401 attagttttg aaaaacttga aaagcaatta aagcacagag gtttaaaaga aaaagataaa
    14461 atggacgaca aataatttat aacataaaaa tgccctcaga taagactaag gatacaaacc
    14521 ttaattcata ttctgagggc tttaatattt gaaattcttg tgtgaccagc atccactact
    14581 aatataaaat tatttgcagt aacactaaaa tccgctgctt tcattttctc gaaatagata
    14641 aatcaactaa tcaatttcaa tttataatca tgtatcgttt gtaactcacc atcgactttt
    14701 cgatatacaa tatgatcagc agtaatttct gtaggactgg atacgccaac agctgctgca
    14761 atattgaata agccttcatg caaacttgtt acatagtttg tgacacgata ttgcttttct
    14821 ccaacaatca atgctttttc ttttttcgca tctgtcgttg caacacctac aggacatgta
    14881 ttcatatggc attgttgact cataatacaa ccgacactaa tcatcatgcc acgtgcgata
    14941 tttacaaaat ctgcacctaa acctagtgca atcgcaattt tatctggtgt cactaactta
    15001 ccagatgccg ccaatttcac tttatctcga ataccatatt tttctaacat gccagacaca
    15061 ataggtagag ctgtaaatag cggtaagcca acaccatctt gtaattcttg gaatgttgca
    15121 ccagtaccac cttcaccacc atcaatcgta ataaagcttg gatacttatc tagttccacc
    15181 atcgtacgta caagtgtttc aatttctgaa actttgctta ctacaatttt gaatcctact
    15241 ggtttttgac ctaattgctg caactgatcg acgaaacgaa tcaaatcttc agcattatga
    15301 ataaattcgt aacggttagg tgaattgatt gttttataag gttcaacatt tcggatttta
    15361 gcaatttctt cgtttacctt ttcagcttcc atatgaccac cacgagtctt agcaccttgt
    15421 gccaacttca gctcaaatgc gcgtacgtta gataactgtg cgacctcttt aaataaacct
    15481 tcactaaaat taccttcttt atcacgaaca ccaaataaac cgggaccaat ttggaaaatg
    15541 atatccccat taccttttaa atgatattct gataagccac cttcacctgt attcatccaa
    15601 gtgcccgctt tagctagacc tttagataaa gctgtaatgg cattttttcc taaagcgcca
    15661 taactcatac cagattgtcc tacgatacgt tttaaaataa atggatgttt taaatgttca
    15721 cctaatttta ttgcatggtc atcacttaag taatacggat caatctttgt cggtacacga
    15781 tattcttcac gactaaataa acgctcattc gcgattttat aaatgaatgt tgataacaat
    15841 gttgtattat ctactgaaat ctcattacgt tgcatcggaa acattgtgtt ctgtatgtaa
    15901 aagccgtctt gataatcttt agtagtaccg aagctggtca tacgagagtt atattttcca
    15961 gccaaaacga tatttttata atcattacgt gaaaaaggtt tcccttcatt atccccagaa
    16021 aataaatact gacgtaattc cggtcccatt ttttctgaaa tatatctaat acgtgctagt
    16081 aaaggataat tccttaatac actatgttgt gattgtcttt tatctttaat taaccaaata
    16141 agcccgataa caataaccgt aagcaggaag cctacaacga taatgttaac tataaattgc
    16201 atgactgtaa gaaacgtcat tacaatacct ccccccaaaa tttcaattca atatttatga
    16261 tacaccttac aaaacaaaac acaatggaag cgcttcattt tataaaacaa ttttatgata
    16321 tgtttttcat tttaaatttt aatgtataaa acatacaata caaagtaata tgtgctaaag
    16381 tatctatata atacaactat ttaagaggta tactatgtca aatacaaata aacattacat
    16441 agaagaagaa tacgctactg aacaatcgcg ttttttcaaa cgtgatattg gatttatttt
    16501 cctttatata tttttggtta acatcttgcc aatcattatc gtctttttaa cttttgggtt
    16561 agtagctgta ttcactcaac aagccccatg gggtttctct tttcctattt caatgaacat
    16621 tagctattta ctcgcagtta ttatttcact agctattttt cgtcggatgc atgaagatgc
    16681 atttgttccg atgatacgta cgcaaatcat tgccagcacc aaatataaat ggcatattct
    16741 tatatcattt acactaagtg tcgtactctt tttactttta aattatttcg atattttcgc
    16801 actaaatgta gatgccttta ctgtctttga catttacccg ctcattagag attatgattc
    16861 catcagttta atagtaatgt tgtggttaac cattatatta aatactttga tttgccaact
    16921 tattttccgt catattttaa ttcaagaatt aagcagatta atacctttaa agattacaat
    16981 agtattatcc gttatccttg aaacactgta ctattatcca tacattaata catggtggga
    17041 attcattcca gcattgatac tagctagtag tgcgacttac ttatatttaa agtcaaaccg
    17101 taattttatg gtgagctatt tctatcaatt agcagttata ctcgttttgc atattttcat
    17161 gtaaaatttt tcagagtatt atccattatc catacacatc aaaaagcaca cttaaaagtc
    17221 agtatcacaa ctcactttta agcgtgcttt atttagatat actgtcgttc taaaaacgag
    17281 ccatatagag agcctgagac ataaatcaat gttcaatgct ctacaaagtt atattggcag
    17341 tagttgactg aacgaaaatg cgcttgtaac aagctttttt caattctagt caaccttgcc
    17401 ggggtgggac gacgaaataa atttttcgaa aatatcattt ctgtcccact cccatgaatc
    17461 gccatgctca atataccttt taaaatttaa atattcatta acttaatatg ctttaattca
    17521 cttcattaaa gactactcgc tggacgtggt gttggctttt tcaagaaact cataatcatt
    17581 attacaatga ctaacatacc acaaattaga tacatccatt taaatcctac taaatcggaa
    17641 ataggtccca tcaatgcgcc acctaacgat atacctaagt ctgcacaggc aataaataaa
    17701 cctaacaaca tattacgacc tacttttggt aagacgaagc ttaagtatga tgttaatgtt
    17761 gggtacacca ttgcttgcgt cattcctatt aatatcgcac taccatagaa aataattgca
    17821 cctacttgcg gaccaaatgc cactacaaat gacgcgatta ctaataatga tagtacagat
    17881 accatatatt taggatgcca cataccatct gacggaatgt atttccttaa gtaaaatctt
    17941 gccgcaacaa ctgcgatggc ctgtattgtc aaaaagattc ccgcattcgc gaatcctaaa
    18001 ctcactgtgt ataacggtac aaatgtacta actgcaccaa atacaatcga tgcaacaatc
    18061 atgataatac cactgttcaa caactcttta tttttgaaaa attgcgcaaa aacagttaca
    18121 gcgttaaacg gcattttttc aatcttatct gacgtatcgg gttcctgttc agcaaaggtc
    18181 acgcgatagc caaagaatgt tgttgttaat gcgataaaga taatgacaat tgcaaatagt
    18241 gaaatattat ttgcattcca aatacctacg gcaactaatg gtccaattaa gtttggaatc
    18301 gttgaaaata gcgagtacaa tgatacacct tcagaacgat gttcctctgg taatgcatca
    18361 ataataccta actgtaaaga catagaaaag aatgccgtac acacaccttg cataacacgt
    18421 gctacgaaat aaccttctaa tccagtaaaa ccataaataa ttaaagctat ggcattgatg
    18481 atcaaaataa ttcttaaaac tttaatggga ccgacacgag caataatttg tcctgcccat
    18541 ggtcgaaaca ccattgctgt taacatgtat gcccccataa cgataccgat aaccgtattc
    18601 gttgcgccta aatcatgccc tcgtaacggt ataaatacgt ttaagattgc attggcacta
    18661 aagaacatca atgttaatat atataatctc aaaaagggcc aagccattgc acctttcatt
    18721 atctcactcc ttagaatgat agttgttttt ctattaattc tcttgtataa gcattgtcac
    18781 ttttatgcaa tgcgcttgtc ggaatttgtt cttctatttt tccgttttta aaaataatta
    18841 attgatcaca taaatacgtg gcagcttgaa tatcatgtgt gataaaaata taactcaact
    18901 gacgcgtttc acgtaaatga atcaataaat ctaatatttg tgtttgaatt gacatgtcga
    18961 gtgaactaat ggcttcatca aacaaaatat atttagggtt aatacatatt gcacgcgcaa
    19021 tcgcaacacg ctgcgcttct ccacctgata acatattagg atatttatcc atgtatgcct
    19081 tagatagacc gacttcttcc aacaatgtaa ttgcttggac ttccataact tctttaggtt
    19141 gtccatcaca ttgacacatc acttcaaata agatttctct aacagtctga aatgggtgta
    19201 atgatgacgt ataatcttga aatacagcac caatttgatg acgtctgact ttcttcttat
    19261 gcatcggtag atcatttaag gttacacagc ctttatccgg tttctcaata cctaatatca
    19321 tacgactcaa cgtcgattta ccgctaccac tttctccgat aatcgcaatc gtcgcaccga
    19381 ttggacactc aaatgacaca cctttcacga taggtgttcg acgacgctta aaaacatgtg
    19441 cgctttgata tgatttttca acatctgtaa ctttaatcat gtacatcacc cctcatcaca
    19501 tgtttaaaat gatcattaat cttcttcttc gttgataata aatacttcgt ataaacatgt
    19561 tctggatgat gcaagactga ttcacgtgta ccatgctcta tcagttgacc atttttcatc
    19621 acaacaacac ggtctgcaat cttgttaata accgttaaat catgtgaaat gaaaatcatc
    19681 gcacagtcaa agtgtttttt aatatctata aatgcttcca gtacatcata ttgtgtaatt
    19741 gtatctaaag ccgttgtcgg ctcatcagca atgattaact ttggtttcaa agctaacgct
    19801 aaagcaatca tcaatcgctg taacattcct cctgataaca tgtaagggta tgattttaat
    19861 atacgtttag gatctttcaa acttaaataa tccatatatt caatcaatgt cttttcaatt
    19921 tcttgtgtag acattgacgt atgtactttc atagtctcaa acatttgttt accgacagta
    19981 gttgatgggt caaaggcacg actaccttgt tgcatgacca tcgcaatatc tttaccacgg
    20041 tactttttcc gttgcgattc agataatgac aacattgatg taccatcaaa gataatttca
    20101 cctgtcaccc cgagtcgttc gggattcaaa ccaataatcg atttgcatgt aattgattta
    20161 ccgctaccac tttctccaat aacgcctaaa gtttcaccct tagttaatgt aaaattcaca
    20221 tcactcacga gtggttgatc tgtccaggta tctgtaatcg tcaaatgctt aactgttaac
    20281 aatgtcatga ttgcaccact ccttttttca cagaacgaag tttatcttta gaagagatgc
    20341 ggggatcaat agcaatttgt aaagcatcgg ataagaagtt aaatgccatc actataatca
    20401 ctatggcaat acctggcgca aacatcattt caggatgtgt aaacatcact tttctagctt
    20461 cgttaagcat catgccccac tctgcagtag gcgctttgac acctaatcct aaaaatgaaa
    20521 agccagatat ttgcaagatc attgaacaca tcgaactact agagatgata gcaatatctg
    20581 ctaatgttaa cggcataata tgtttgtgaa taattttcat atcattcata ccgattgttt
    20641 tagcaaatct gacatggtca gaagcagtgt actgcataac acttgtacgt ataacacgac
    20701 agaaccatgc ccaacgcgtc aaaataaatg ccatgataat attttcggca cccattccaa
    20761 acaatgcaat taatgctaac gttacaacat aacttgggaa tgccaacata acatcacacg
    20821 cacgcatgat taaggcgtca acaaaccctt ggaaatatcc tgataagaat cctaaaatag
    20881 atccaataag tacagaaaca aatagcgcaa caaagacata taacaaactt ggtctaatcg
    20941 cataaattaa cctagttaaa atatctctac ctaaatggtc agtacctagt agatgttgaa
    21001 aactgatgcc agcaaatttg tttgctgtat cgatatggtt aggatcataa aatatcacaa
    21061 gtggtgctgc taatcctaaa aagacatata atacaataat gcctaaagca attactgcac
    21121 ctttatcttg taataatcgt tttaaaatta tcatcgtgcg ccctccctta atcttggatt
    21181 taatagcgca ttaatgatat ctgctaatgt attaaataca ataaataata ccgctacaat
    21241 taatacatat gcttgaatga ctggaaaatc gtgttcaagt attgctttta aacttaattg
    21301 acctagtcca ggccatgcaa agatatactc gataacaact agtccaccca ttatcattgg
    21361 tatagacata caaaagattg ataccgcaac ttgtatagca ttacgcaaca catgcaacat
    21421 taatgtgata gatttcacac cgcttgctct taaataaagt acataatctt catttaattg
    21481 ttccaccatc gagcgtctaa catttctaaa gtaaatacca gcataggcaa tcgtaataac
    21541 gatcactggc aatatgtaac tttctgggcc tgttaatcca gaagtcggca atatgtttaa
    21601 cttcactgaa acgtaaataa taagtattga agctatccaa tatgatggta atgcagttag
    21661 aaagaaagcc actgaacgta tcgcacgatc agtgaacttt cctcttttta atgcactaac
    21721 tacacctaaa ataattgatg taatcatcac cataacactt gaaattattg ttaatttcaa
    21781 tgtgttcata aatgctgggc caatgcgttc agcaacagga tcccctgtaa tgtaacttgt
    21841 accaaaatta aattgcatcg cttcaagtaa ccagttttta tattgaatta ataatggatc
    21901 attgaaaccg tacttttcat tcgtttctgc aatcaactct ggtgttacat ttggcgttcc
    21961 ttgtgcatgt aaaattgtca cagctggatt ttcatttgta atatacgtca atagaaatgt
    22021 cataaaactt actacaatca ctaatggaaa catgagcgca atacgtttta agataaattt
    22081 gaacatctaa ttgctccttt atttatactg catttcattg aatggtaatt catactgtga
    22141 ttgtgtgaat gatacttttt ctaaatcttt cggcgcaaca actgtcatac taccgtgtga
    22201 aatagggata aagatacctt catcatcaat ttgtttcaaa atgtttttat aagcgtctga
    22261 acgctcttta ccgttttgga ttttaaatgc gtcatcaatg ctgttgtata ttttatcttt
    22321 gttctcaatg cctgatgttg cactttcata accatttttc gctttaaatg ctgcaatagt
    22381 actttgtgga tcgtacaata atccccaagt ttggttgaac attaagtcat aatcaccaga
    22441 agtacgacgt tcagcaattt tatctgatgt ttcgccattg atgtttaact taatacccat
    22501 tttcttaaat tctgcttgta agtattctgc ttgttctttt tgacttgaag aacctttgtc
    22561 atagtacatt gccatttcaa ggtttttacc atctttttga cgaacatcac tgtctttacc
    22621 tttcttccaa ccagcttcat ctaataatga ttctgctttt ttaaggtcat acttacgtgt
    22681 tggcatatcg aaattaatgt ctgttacatt tttcgcaaat aattgagttg ctggtttttc
    22741 ttgaccatct aaaatttctt tggcaatttt atctctgttt accatatgac caatcgcttg
    22801 tctgactgtt ttgtcactga cagcgttatc ttttttacca gaattgacaa ctaacatttt
    22861 cgtattcata ggttgactac gcttaacttg atagtcacct gtatctttca attgttttaa
    22921 agagtctttg tctaagctat ccgtacctct atcatctgtg aaggcaaagt tcgtttcacc
    22981 ttttttcatt gataggaatg ctgtttcacc agcaggcatt acttttgctt gtactttgtt
    23041 aagtttagac ttttcgcccc agtattgatc atttttgtta aagtctgcag actcatcttt
    23101 tttgtgttca cctaatttaa atggaccagt accatcgaac tttttaacgc catcttttgt
    23161 tgtaccgttt ttaaagtctt ttggagacac aaatacatat ggacgaggca tcgctaattc
    23221 agccaatgca ggttgatatg cttctttcaa attcaattca accgtgtact tatctttaac
    23281 tttaacattg tcaattaatg ttgaaatctt taaccaagaa tgcaattttt tattttgttg
    23341 aaccgcatca atatttttct taactgcgtc agcatcaaat gttgttccat catggaattt
    23401 cacatcatct cttaaatgga atgtataagt cttcccatct tcagacacat cccatttttt
    23461 agctagtaaa ggcttaatac catctttcgt gttacgtaca agcggctcgt atatcatact
    23521 ttcagcagac attgatccac cgtaaacatg cggattcata tcaccgatat ctttaaccgt
    23581 cgtatacgtt aattgcttgt tttctttttt ctcctctaaa cctttattac cgccacaacc
    23641 agttaaaatt agccctgatg caagtaacat tgcactcatt ttagttagtt ttctcatttg
    23701 cttttcctct ttctaaattg ataagttgca ttacttaatc aaatcgtaat gattattttt
    23761 ctcaattatt tgcttatttt tatgtaaatg tcaaacataa ttcaataatt tttttctatt
    23821 ataaaacttc aaacccttat tatgaaagcg ttctattgat ttccaaaaat tttgtgacga
    23881 gtgccttatc tccttcaaat agatttgtat taaattgaga agatagttgt tgatcttggt
    23941 gcatatcctt gtacgcctgg caacttgctt catagcgagt taggaactga tcaatcattg
    24001 gcgttttgat acctaacatt ttcccaatat gctgaatcat cgccgttctg taataatctt
    24061 cacttggcat tcttggaatt tgaacaacat cctgttcatt tttatagact tgcttaaatg
    24121 gtacagctga aaaatcaaag taatgtccgt tttcgtctgg ctgtgaaaat ggatcaatga
    24181 ggattgcggt gtatcttaca taaagcagat attcttgtaa gatatctggc aagatttcga
    24241 aatgctcaat atcaccttca tccaaagttt caggacgtac tggataattt tccttcacca
    24301 taaattgaag caggttgact gacggcactc taaatgcttg taaaatagcc atcatttcct
    24361 tccacattaa acgcatttca cggattaatg tcatcgttat cggtccttca ggaaataact
    24421 tatacacata aaccggtaca tccgttcctt cgaaaatggc tttcaatgaa aagtcattca
    24481 taaatagtgg cgggtgcaca taaagcgaac tgttacgtgt ttcagcatgc agtggcgatt
    24541 caacgacttc taattgaatc ttcaattgct cagctaaagc agagattcgt tgacacattg
    24601 ttgagtttga atgtgtcgat cccatgtaca atttcttttt tacacctgtt gtcaacacat
    24661 gattaggtgc ttctttatca acaatacgtg tatcgccaag ataagttgaa aatgaaatca
    24721 cttcgatatc ctgactgaac ttagacataa attgttccac aatcatttgc gatccaaatg
    24781 tcggtgaaat taaaatgaca tgtttgacac tttgcaaagt ttctaacgac aattgctgta
    24841 gtgtgtcata ataagcatct gctgtgcatg ccatgacaac cgtttcgtat tcacccttaa
    24901 cgtttttaac atctttatac aaacgattaa tttcaaactt accttccaga tgttgatgcg
    24961 cctcgttttg tattttgact tcaaattgtt tctctttttt atacgcttga tataagcgtt
    25021 ttgatttttc tgatgttgag gcacgtccaa ccatatcaat ctcataatct gattttaaat
    25081 agcaaatatt cgctaattgg attgcgaccg gaccagtgcc tatcattaat aatttagaca
    25141 tccgtaatac ctacctttat agctgctttt ttataaagtg ctatatcaaa aatttgctgt
    25201 ggtctcatat gtttattcac acattgccac ttatcttccg ctgtttcttg tgacggataa
    25261 ttaaatattg ctttgatgcc atcaccaaag cgcattgcaa ctacgacatt ttcatttgtt
    25321 aaatcatata attcttctaa aatgctgtac tttaaaggaa ttgtcgagct gaatatgata
    25381 tgcgtcacat ctttgatatc tttaagttca gatacctttt gatccgtaat tgttatatct
    25441 tcatttggtg ctaagacgtt aacgattctg cgccctaggt caacggcttg tggatcaata
    25501 tcaataccga taactgaagc acctgtttct tttgctactt gaattaacgt cattggatat
    25561 gcacctgaac caactaacaa taatttgtca ttgctagtga tactacattg tccaaactct
    25621 tcatcaatac aatgttctat attgtcaaag taacctgtgg tagacgcctg tccgtctaat
    25681 agacgtctag ctctaatgac ttccacttgt ttaacacact gcgctgtaat atattgcagt
    25741 gattttatta attgtgcttt ttcttcaacg tcacgccaag cattaaattg ttgttcataa
    25801 ataggattta aaataaattc actatagtca tccatcaatg tttctaatgc ttcgatatat
    25861 tgatcgtctt gtaatacacg ctcgtaatgc gcttcaaact tttccaaata ttgttgtaat
    25921 atcaatttga tttcattatt aaagttattc attgtaggct ccttattcta tatatgcttt
    25981 tcctgtagct acagttgtaa cctgtccttt aattgaagtt tgatatccca attgatgaca
    26041 tcgctttgat gtcactaaaa tactgccccc tggctgatgt actgtaaaat ctttgcatgc
    26101 gtcattacgt tgataattat taaaaacccc aattgatgct gtaccagaac cacagctatt
    26161 ttcccaaatt aaactttgaa tttctggtat atagattaat ggctgtaaaa attgacgttg
    26221 ttcatcaaaa agcatcatac ctactgtttt atatttgtga ctccattgtt gctcacgcac
    26281 aaatgcttca accaaatgtt gaatttcagt tgttacttgt ttaactggaa tcacataatg
    26341 tacatatgtt tcataaataa tttctaatgc tttccatgaa tgattaccca tattaattgt
    26401 tgttggcaca acacgatggg cttgtggcat ttgaacttca tagtattggc aatcatgaat
    26461 tgcgcattgc actaaatccg aacagccaga taccttcacc ttaaactgtt ggtctttaag
    26521 caaatgactt tcctgcaaat gatgtatata tgacatcgtc gcattaccgc aaaattcatt
    26581 accgctcata actaagtgaa aatcattgcc atcatcattt tgtgttgatt ctataaagcc
    26641 tacctgttca caacatacat gtgttgcggc catcaactga ttggcgatag atgcatattc
    26701 actagcatca tgttttgaat gaacaagtat cgtcatattc cccgaaggat tatacttaga
    26761 aaattctata acctgccgat tcatacgaca ctcctttaga tgtattttta gagcctcttt
    26821 taccataagt aattagaggc tcttatgcag ttggcgcgaa gcaagcaact gtattcctct
    26881 aaaactgtaa tttttagaag atgaacctaa tattttccca tctattaggt aagtcatgct
    26941 aacccgcaaa agcgaaaacc atggctctta tgcagttggt gcgaagcaaa caactgtatt
    27001 cctctaaacc ttgtaatttt tataaaaaga acctaatatt ttcccatcta ttaggtaagt
    27061 catgctaatc cgcatagtgg aaagcatgac ttagtaatcg gttgtttcat caacatgcaa
    27121 ccgtaaacct tatttaaagt tgcttcttgt ggctctacaa gtgaatgcag ttgtccgtta
    27181 acttcattca cttagatgct tttactgttt tcacctcaac taaaacttca aaaaagttgc
    27241 agaaaacaaa tcgtaactat tacgatttga ttatatacta tattagttga gaatcattgt
    27301 caataaaggt tgtaaatgaa tgcacattta tattttgata atctttccat aaacatcgta
    27361 ttctcagcac attcaagtct tttgaaaatt tttatcaaac aaaaaaacca gcttcaacgt
    27421 aaaatacgtt aaaactagct ttaagcaata cacttcatca aataaacaaa catttatttt
    27481 tcaaaggtta ataattcccc gtcatttggt acaacaactc tattacctaa gtcgttagaa
    27541 tttataaatt cattcagctc tttacgagat aaatgccaat gattgaccgc ttccatatgc
    27601 acaacaacaa tttgcgcaag tggcaatgta ttagcaactt ttaacacatc atgttcatcc
    27661 ataactaacg gcccaccttc aaagaattga tttgcaccac cattaagaac aacaacatct
    27721 ggttgatacg tttttaatgc tttttcaacc ccttcaaacc aaactgtatc tgctgctaag
    27781 tataatgtcg gctcttcaga atgttttaaa atcatgccgt gaacatgtcc tgtcatcttc
    27841 aaaatttcac catgaccatg ttgagcatct gttttaatca actcaatttc tccaatgtgt
    27901 gtcacatcat taaatacaga aacatttgta aacccataac gctctacttc actagcatct
    27961 tcttcatttt gtgcgtaaat tttaatatct ttcggcaata cttcaattgc tttcggatca
    28021 aaatgatcaa tatgcaaatg cgttaaaatc actaaatcga tgtcatttat aatgtcttca
    28081 attgccatgg gtagttctac aagtggattt ttttgatctt gtctcggtga atttggaaaa
    28141 gggtcaaatg aacctttatc acttaacatt ggatcaatca aaatgcgttt acctccatac
    28201 tcaatcacgg ccgtggcatt tcttatatgt ctaatattca tgttaaatcc ccctatttca
    28261 tatgtctgtc attgcgacaa tatgcataca ttgcctactt catgccaaat tcttttccac
    28321 tggatatcga ttttaaataa ttattccaat gtccgcttcc acgaatcatc acttaacatc
    28381 tcgccaggcc atgtgtaagc catcacacca ccatcaattc gaatcgtctc tccagttatg
    28441 aatgaactgt cgtcagatgc taagaagact actaatttag caacttcttc tggtttacct
    28501 aaacgtccca gcggagtcat ccatttttga ttttctctaa atgttttacc tgcatcatcc
    28561 tcactcgtac ctgtcagttt atctactaac ggggtttcaa ttgtacctgg tgcaattgca
    28621 ttggatcgaa tgccatcacg accatactca attgcgattg attttgtaaa attaatcact
    28681 gcgcctttcg cagcattata tcctgagcga tacaaatctt ctgcctgtcc tgaaaatgaa
    28741 gacgtattaa caatagagcc accttgattc atcattaaag gtaacatcat tttcgtcatt
    28801 aaaattgtcc cacgcatatc tacattcata atcttgtcat acacatctat tggatactca
    28861 tgaattctac cagccgcatt atccacaccg gcattattaa acaacacatc gattcttcca
    28921 aactgttcct ttatgtcaga cacaaagtct accacttgtt gttcatctga aatatccaca
    28981 ttatacgcct tcgcattgtc accgttactt ttaattttat cgacagtctc cgataccgct
    29041 tcagctatgt ctaccgccaa tacatacgca ccttcttgag ctaaagcgat tgcagaagct
    29101 tgtccgatac ctgtacttgc tcctgttacg actgcaactt tattttccaa acgtttcatt
    29161 cagtcactcc tatacaacat atcttaaact tacagtcctt attttccact gttcaatgct
    29221 tcataaacct aatagataag tctccaggca gtcttagcta ctctctgcta tacaatttct
    29281 attgaataac tcaaaatatt taacaatatt agtcttttca ttttctgaaa attgttactt
    29341 ttgagagtat tttttgttac actttaaata tttaacaaag gggggaatca ttgatgacat
    29401 caaaacatca acaaaagggg tctatcgtca atcgtttctt gaatagtgtt gaaaaaattg
    29461 gaaataaatt gccagatcct agcgtcttat tctttttaat gtgtgtgggc ttagccatta
    29521 tgacgtgggt tatctcatta tttaatgtat ctgttaagca tccaggtacg catcaaacca
    29581 tttatattaa aaatataatt agccatgatg gatttacgat gataatgaat gatacgatta
    29641 aaaatttctc agagttccca gcattaggct tagtactagc agtgatgatt ggtattggtg
    29701 ttgcagagaa aacaggatac ttcgataagt taatgatttc tgttgtgaat cgcgcaccac
    29761 gatttttaat tttaccgact attatattaa ttggtatttt aggtagtaca gccggcgatg
    29821 ctgcgacaat tatcttgccg ccgcttgcag caatgctttt tattaaaatt ggctatcacc
    29881 ctattgctgg tttaacgatg gcatatgctt ccgctgttgg aggatttgca gcaaatatag
    29941 ttgttggtat gcaagatgct ttggtctatt catttacaga accggcaaca cgtatcgttt
    30001 cagattctat taaaacaaac gttgctatga actggtactt tatcgcagcg agtgtcgttg
    30061 tattacttcc tacgatattg ctagttacaa ctaagctcat tattcctaga ttaggaaaat
    30121 atgatgacag tttaatgcat gatgaccatg aagaagcatc ttcacatatt actgacaaag
    30181 aagcacatgc tttaaaatgg gcaaatatca gtttcatagt cacaattatt ttattaatta
    30241 ttacagctat tcctgaacat agttttttaa gaaatgctaa aacaggcagc ttactagacg
    30301 atgcgccatt aattaacggt gtcggattaa ttattcttgt cgtattttta gtacctggat
    30361 tagtttatgg gattttaagc aaagaaatta aaaatacaaa agacttaggt aaaatgttcg
    30421 gcgatgctgt aggatcaatg ggaacattta tcgttattgt gttttttgca gcacagctac
    30481 ttgcgtattt aaagtggagt aatttaggaa ttatcgctgc tgttaaaggt gccaaattat
    30541 tagaacatca aaacggtatt gtattgatac taggtattat tgtactgagt gcaatggtca
    30601 acatgttaat cggaagcgca tcggctaaat ggggtatttt aggaccgata ttcgtaccaa
    30661 tgttaatcct tatcggcttt catcccgcat ttacgcaagt catttaccgt gtaggcgatt
    30721 caattaccaa tccaattaca ccgatgatgc cgtacttacc tttattatta acttatgcac
    30781 aaaaatacga taaacgcatg aaactcggag ccttactttc tagtttaatg ccgtattcaa
    30841 tcgcgctaag cattgtatgg acagtatttg tcatcatttg gttcttatta ggtatccctg
    30901 ttggaccagg tggaccgata ttcgttaaat aacagtattc aaagggataa attaaaaatg
    30961 ccactataca agatttgacc tgtatagtgg catgatttgt tatgatatag cgttcgagat
    31021 gaaactctgc atttatatga gcaattatag tctatcacta ataatattta gtctgttcca
    31081 accgttaatt tggtttacaa caaagacaag atcaataatt tgttcttcat cataaaatga
    31141 ttttagccgg tcaataattt caaacttctt aaagtctttg attgaattta acatttctgc
    31201 aaattcaagc gtcacttttt cttgatcagt aaataaatct aaatgtctaa aactcaagac
    31261 ttcatcaatt ttcatttgtg ttacacccaa ttcctttaat tcttttttat ggatatcaac
    31321 acaataatga caaccgttaa tttttgaaat taataactta atcaatgcac taagttcatc
    31381 attaaccggt gattgatgaa taaacttttc accttggctc attacagaca ttaactctcc
    31441 attttgtttc ttgtaattat acggcatttg caatctctcc tttacattgt ttaataccaa
    31501 tccagtgata ctacacttcg aacaatattg gatagaattt aaaattctgt ataacatctt
    31561 gtcaatctat gctttagtta aaacatttag cgcttatatt taaaaaatat ataaaatatg
    31621 aaaacagatt gaaaggatat tataccttgc tctacccttt tttataaaaa atcacacaca
    31681 tttgagaaaa tttgataatg tcaataaatc acgtcatcgc cattgttatt cggtatataa
    31741 tgtttttata atggctaaac tattatttca tttacattaa tggggttcgc ttgtgttaat
    31801 gtactgtaca aaactgggga agtattggtg atgatgaagt gattgatatt aattgcgaaa
    31861 caccttcgat agcaatgaca tagattattg gaagattaaa tcaaaaataa agaagaccct
    31921 agtggccttc ttttttgtct aattcttctt tgatttcgtc aggtgatttt aattgatgcg
    31981 ctggttttag taactcactt aatctttcac tttctatcat agcaccacca aactgataat
    32041 tatgttctgg tgtagcataa gcagtaaaat tatctttttt gttttcattg atgtagcctt
    32101 caattacggc atcgcccatg ggtcctcttt ttaaacttgt gaaatgcaca gatttgatgg
    32161 tattgggttc tttggtatta tgctg
//
LOCUS       DAHHSC010000010        31976 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.010, whole genome shotgun sequence.
ACCESSION   DAHHSC010000010 DAHHSC010000000
VERSION     DAHHSC010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 31976)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31976)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31976
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.010"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            complement(<1..821)
                     /locus_tag="NBI57_000359"
     CDS             complement(<1..821)
                     /locus_tag="NBI57_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HCD1821344.1"
                     /translation="MNQYNTIGFHPGNSRIHQLNATVKLLFLLVVSISAMVTYDTRYL
                     ILISASSILLFKYAHIEWKQVRFVVKFILFFTILNIIAVYIFDPEYGVKIYNQRTELV
                     NGIGRFTLTSQELFYLFNLILKYISTVPLALIFLFTTNPSHFAASLNQLGVNYKISYA
                     VSLALRYIPDIQETYFNISQAQQARGYDNSKKAKFTSRVKGIKRIVLPLIFSSIERID
                     TISTAMELRQFGQYKRRTWYVKKQLKKDDYVVLCLTLILLMLVVTLFFLNNSRYF"
     gene            complement(818..2530)
                     /locus_tag="NBI57_000360"
     CDS             complement(818..2530)
                     /locus_tag="NBI57_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCD1821345.1"
                     /translation="MTEPIISFKDFSFQYHSQATPTLQNINVDIYPGEKVLVVGASGS
                     GKSTFANCINGLIPFKTKGNITGELYINNQDATVSCLHDRSNVVGTVLQDTDGQFIGL
                     TAAEDMAFLLENNCVEQDDMKKNVSYWAEKVGMIEHLNHRPQDLSGGQKQRVSLGGIL
                     IHRTPILILDEPLANLDPATGHETLRLLNNIHEETKSTMIIVEHRLEESLDDTFDRVL
                     LFKDGKIIANTTPSDLLKSSKLKEAGIREPLYCTALKYAEVDVESIDNLANLRDVCMS
                     EHVKFKVKKWIDETSANNDNKYKSEPLLELNEVCVQYSDYSNSVLNNVQLNVYRREML
                     SIVGHNGAGKSTLAKAICGFLDITGNIQFCNRGFNQLSISERSEFVGYVMQNPNHMIS
                     EKMIYDEVALGLRARGMKESDIKIRVENVLKICGLYAFRNWPIAALSYGQKKRVTIAS
                     VLVLNPEIIILDEPTAGQDFYHYNEIMSFLIELNRQGKTIIMITHDMHLLSEYSSRTV
                     VLSKGQVVADTTPVLILNDKKICEIASLRQTSLFEMAEYIGISEPQKLVQLFINHDRK
                     VRRQ"
     gene            complement(2583..3137)
                     /locus_tag="NBI57_000361"
     CDS             complement(2583..3137)
                     /locus_tag="NBI57_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF-type riboflavin transporter
                     substrate-binding protein"
                     /protein_id="HCD1821346.1"
                     /translation="MKKQDISVKTVVAIGIGAAVFVILGRFVVIPTGFPNTNIETSYA
                     FLALISAIFGPFAGLMTGLVGHAIKDFTTYGSAWWSWVICSGIIGCLYGWIGLKLNLS
                     SGLFSRKSMIYFNIGQIIANIICWALIAPTLDILIYNEPANKVYTQGVISAVLNIISV
                     GIIGTILLKAYASSQIKKGSLRKE"
     gene            complement(3166..4014)
                     /locus_tag="NBI57_000362"
     CDS             complement(3166..4014)
                     /locus_tag="NBI57_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosyl-l-methionine hydroxide
                     adenosyltransferase family protein"
                     /protein_id="HCD1821347.1"
                     /translation="MGNYLVLQSDFGLSDGAVSAMYGVAYTVSDDIRVENLTHDIPPY
                     DIWVASYRLYQTVKYWPKGTVFVSVVDPGVGSDRRSIACLTYSGHYIITPDNGSLSHI
                     KHYEGIKKVIEIDEVKSRLPHSEESHTFHGRDVYAYNGARLAANKIAFERLGQAINVD
                     SIEALEIREATKNEDSVTGSIDILDIRFGSLWTNIPLAFLKSLEIVHGNNLVVTIYHQ
                     DKKVYQNIIKFARSFADVNVGEPLVYVNSLVNIGVAVNQDSFSDLYHIGTGNDWTIHL
                     SKAPSI"
     gene            4393..4908
                     /locus_tag="NBI57_000363"
     CDS             4393..4908
                     /locus_tag="NBI57_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyisoprenoid-binding protein"
                     /protein_id="HCD1821348.1"
                     /translation="MTNFTFDGAHSSLEFQIKHLMVSKVKGSFDQFDVAVEGDINDFS
                     TLKATATIIPSSINTKNEARDNHLKSGDFFGTDEFDKITFETKSVTENKVVGDLTIKG
                     ITNEETFDVEFNGVSKNPMDGSQVTGVIVTGTINRENYGINFNQALETGGVMLGKDVK
                     FEASAEFSISE"
     gene            complement(5059..6030)
                     /locus_tag="NBI57_000364"
     CDS             complement(5059..6030)
                     /locus_tag="NBI57_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HCD1821349.1"
                     /translation="MSQQDLPTLFYSGKSNSAVPIISESELQTITAEPWLEISKKGLQ
                     LEGLNFDRQGQLFLLDVFEGNIFKINPETKEIKRPFVSHKANPAAIKIHKDGRLFVCY
                     LGDFKSTGGIFAATENGDNLQDIIEDLSTAYCIDDMVFDSKGGFYFTDFRGYSTNPLG
                     GVYYVSPDFRTVTPIIQNISVANGIALSTDEKVLWVTETTANRLHRIALEDDGVTIQP
                     FGATIPYYFTGHEGPDSCCIDSDDNLYVAMYGQGRVLVFNKRGYPIGQILIPGRDKGH
                     MLRSTHPQFIPGTNQLIICSNDIEMGGGSMLYTVNGFAKGHQSFQFQ"
     gene            6274..7230
                     /locus_tag="NBI57_000365"
     CDS             6274..7230
                     /locus_tag="NBI57_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HCD1821350.1"
                     /translation="MNYQVLLYYKYTTIDDPEQFAQDHLAFCKAHHLKGRILVSTEGI
                     NGTLSGTKEETEQYMAHMHADERFKDMVFKIDEAEGHAFKKMHVRPRKEIVALDLEDD
                     VDPRHTTGQYLSPVEFRKALEDDDTVIIDARNDYEFDLGHFRGAIRPDITRFRDLPDW
                     IKENKALFTDKKVVTYCTGGIRCEKFSGWLLKEGFEDVAQLHGGIATYGKDPETKGQY
                     WDGKMYVFDDRISVDINQVEKTIIGKDWFDGKPCERYINCANPECNKQILVSEENETK
                     YLGACSYECAKHERNRYVQANNISDNEWQQRLTNFDDLHQHA"
     gene            7382..8020
                     /gene="pcp"
                     /locus_tag="NBI57_000366"
     CDS             7382..8020
                     /gene="pcp"
                     /locus_tag="NBI57_000366"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HCD1821351.1"
                     /translation="MHILVTGFAPFDNQNINPSWEAVTQLEDIIGTHTIDKLKLPTSF
                     KKVDNIINKTLASNHYDVVLAIGQAGGRNAITPERVAINIDDARIPDNDDFQPIDQAI
                     HLDGAPAYFSNLPVKAMTQSIINQGLPGALSNSAGTYVCNHVLYHLGYLQDKHYPHLR
                     FGFIHVPYIPEQVIGKPDTPSMPLEKIVAGLTAAIEAISNDEDLRIALGTTE"
     gene            complement(8139..8906)
                     /locus_tag="NBI57_000367"
     CDS             complement(8139..8906)
                     /locus_tag="NBI57_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1821352.1"
                     /translation="MLTKQSQDFLEKLRVELLFRGKTEAEVEELLEELEDHLTMAEKN
                     NEDTSSIINTPIKSMADQLSPEISLTTGLYKYITLYFSFILAVIIIPRFLDLGTFDVT
                     IAFLLYIASIVILGVVIGMLILRNTLVRFGDQKITYVINVTYGIVVFLWMIFGGLIIK
                     KYPIYSFFELSDKQSFIVGLVLLIALIVICILIKQKIYALIVLVISLPSLIARIAAIF
                     SKEPDTFNLVSVSILIILNIAFIIYTFVNFRKLKKED"
     gene            complement(8896..9234)
                     /locus_tag="NBI57_000368"
     CDS             complement(8896..9234)
                     /locus_tag="NBI57_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000116416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HCD1821353.1"
                     /translation="MSVSDQIMKGLLDGAILGLIAQGETYGYEIMTKLKNQEFPEISE
                     GSIYPVLMRLNKKGYVDTTIKKSSNGGPRRKYYTITDSGLKELKVFKSKWQALNKGMI
                     NLFGGENDVN"
     gene            9401..9865
                     /gene="bstA"
                     /locus_tag="NBI57_000369"
     CDS             9401..9865
                     /gene="bstA"
                     /locus_tag="NBI57_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacillithiol transferase BstA"
                     /protein_id="HCD1821354.1"
                     /translation="MTTKTVFDVIDMGLGYLVNVYDAWKVEKVLDDYHEPFSNTIHWQ
                     FGHVLTIFESALAVAGKENIDLNIYRPLFGNGSSPDEWKDEVPSIEKILEGLQTLPER
                     ARNLTEDDLAIELKQPIVGCNNLEELLVLNAIHIPLHAGKIEEMSRILKNLK"
     gene            10103..11521
                     /locus_tag="NBI57_000370"
     CDS             10103..11521
                     /locus_tag="NBI57_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion permease"
                     /protein_id="HCD1821355.1"
                     /translation="MENTVKYRKFILPIVVGLLIWALTPFKPDAVDPTAWYMFAIFVA
                     TIIACITQPMPIGAVSIIGFTIMVLVGIVDMKTAVAGFGNNSIWLIAMAFFISRGFVK
                     TGLGRRIALHFVKLFGKKTLGLAYSIVGVDLILAPATPSNTARAGGIMFPIIKSLSES
                     FGSKPKDGSARKMGAFLVFTEFQGNLITAAMFLTAMAGNPLAQNLASSTSNVHITWMN
                     WFLAALVPGLVSLIVVPFIIYKIYPPTVKETPNAKSWAENELATMGKIALAEKFMIGI
                     FVIALTLWIVGSFIHIDATLTAFIALALLLLTGVLTWQDILNETGAWNTLVWFSVLVL
                     MADQLNKLGFIPWLSKSIATSLGGLSWPIVLVILILFYFYSHYLFASSTAHISAMYAA
                     LLGVAIAAGAPPLFSALMLGFFGNLLASTTHYSSGPAPILFSSGYVTQKRWWTMNLIL
                     GFVYFIIWIGLGSLWMKVIGIF"
     gene            complement(11935..12843)
                     /gene="rarD"
                     /locus_tag="NBI57_000371"
     CDS             complement(11935..12843)
                     /gene="rarD"
                     /locus_tag="NBI57_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HCD1821356.1"
                     /translation="MNIEYKKGIFLALSAYILWGILPIYWQFVDAIGAFEILAFRIIF
                     SAIFMIFILAVGQKQRNAFQRDMNQLLGKPIQLLAIVVAGYVITLNWGTFIWAVTNGH
                     VLQTSLGYYINPLVSILLALIFLKERFNKFEWLAILFAFIGVLYMTLKIGEFPIVSII
                     LALSFGTYGLLKKVVHIDAISSITIECIVTAPAGLIYVIYLWQQHQMSFGLNMSSFWL
                     LFSGAITAIPLILFSAGAKRIPLSLIGFIQYVGPTIMFVLGIFVFKEPFSIDQLITFI
                     FIWTGIVLYSLSQYIKLKKHPVAKTL"
     gene            13065..13640
                     /locus_tag="NBI57_000372"
     CDS             13065..13640
                     /locus_tag="NBI57_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000174573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HCD1821357.1"
                     /translation="MTLPKIGKPATRALNSQGIYTLEAVSQYTKSSLMEMHGVGPKAI
                     SILEQALFQHQLHFKTEVQSSLPFKLTGDVSCNHAPKRQQMIDFIVATAALDIELLRS
                     LVTTEFIWSVPGHFDIYGPQILIQELSNYYNQVASLNIHSNITHGCLGSMHGIEILKT
                     GKEIHFAHFFEFENHKKDAKLSKVTSYIVVA"
     gene            13804..14820
                     /locus_tag="NBI57_000373"
     CDS             13804..14820
                     /locus_tag="NBI57_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HoxN/HupN/NixA family nickel/cobalt transporter"
                     /protein_id="HCD1821358.1"
                     /translation="MTVFKNERLSWLPYITIVILLHVIGFSFLWIAGKDHHILFGMGI
                     LAYTLGLRHAFDADHIAAIDNTVRKLLQQRKDPSGVGFYFSIGHSSVVFLMAVFLGIS
                     VKWAKDELPHFQDIGGTIGTLVSGFFLVLIGVLNLIILISLINLFAKLRREHIEEAEV
                     DVLLESRGFVSRFVGPYFKLITRSWHVLPLGFLFGLGFDTASEIALLALSSGASQQAI
                     SFIGILSLPILFASGMSLLDTLDGVVMKYAYNWAFFNPIRKIYYNITITAISVMAALV
                     IGMIELLQILADKLDLHGAFWAFIGSIEFDYLGYILVALFLITWLISSLIWKFGRIEH
                     KWSK"
     gene            15063..15857
                     /locus_tag="NBI57_000374"
     CDS             15063..15857
                     /locus_tag="NBI57_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylamine N-acetyltransferase"
                     /protein_id="HCD1821359.1"
                     /translation="MNIAKLENYLQIDSSRYNRPSIEALNYYATRFMLTVPFENIDVQ
                     NSKPISINIDALFNKIVHDKRGGFCYELNTFFKAYLQQKGFNPELMSATIHTPGGGRS
                     LNGSHASLVVSINDVFYVTDVGFGDLPLHAIPITSSEHTQPITDISGTFRAIFNNEDK
                     DIFYVQKFENDHWHTKYEAEFKPKQIEDFNSNIEYNQTHPDSIFVQHLLITMPQSFGR
                     ATMSENHLTLTRNGSSEKFDVTKDNYKHFLEKYFGLNVTINRIEKQ"
     gene            complement(15991..16503)
                     /locus_tag="NBI57_000375"
     CDS             complement(15991..16503)
                     /locus_tag="NBI57_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HdeD family acid-resistance protein"
                     /protein_id="HCD1821360.1"
                     /translation="MEKGNQGIKWSSLIMGVLLLMLAVVIFTFPIENFYAITWLIGLF
                     VLINGVIQIIYRRKAKALVGGNQNWILFMGIVDILFGLIVIFNVGASSAFFIYMFAFW
                     FIFSSIAGLFTFTGSGSLKLISVIFNLLGIVFGVILLFNPLMGIVFISTMIAIAFVFV
                     GVIYVVDALA"
     gene            16786..17544
                     /gene="vraD"
                     /locus_tag="NBI57_000376"
     CDS             16786..17544
                     /gene="vraD"
                     /locus_tag="NBI57_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide resistance ABC transporter ATP-binding
                     subunit VraD"
                     /protein_id="HCD1821361.1"
                     /translation="MTILSVQHVSKTYGKKHTFQALKDINFDIQKGEFVAIMGPSGSG
                     KTTLLNVLSSIDQISSGSVIANGQELNKLNQKALAKFRKESLGFIFQDYSILPTLTVK
                     ENIMLPLSVQKMSKATMEENYKAITTALGIYDLGNKYPSELSGGQQQRTAAARAFVHK
                     PQIIFADEPTGALDSKSANDLLQRLEEMNKSFDTTIVMVTHDPVAASFAERVIMLKDG
                     QIHTQLYQEGRSKQAFYEDIVHLQSVLGGVSNDI"
     gene            17534..19414
                     /gene="vraE"
                     /locus_tag="NBI57_000377"
     CDS             17534..19414
                     /gene="vraE"
                     /locus_tag="NBI57_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide resistance ABC transporter permease
                     subunit VraE"
                     /protein_id="HCD1821362.1"
                     /translation="MTFNHIVFKNLRQNLKHYAMYLFSLFFSIVLYFSFTTLQFTKGV
                     NNDDSMAIIKKGALVGSIFLFIIIVIFLMYANHLFVKRRTREFALFQLIGLTRQNILK
                     MLALEQMIVFLITGVVGVLCGIAGAQLLLSIVSKLMSLSINLSIHFEPMALVLTIFML
                     IIAYVLILFQSALFLKRRSILSMMKDSIKTDATTAKVTTAEVISGVLGIAMIALGYYM
                     ATEMFGTFKALTMAMTSPFIILFLTVVGAYLFFRSSVSLIFKTLKKSKNGRVSITDVV
                     FTSSIMYRMKKNAMSLTIIAIISAVTVTVLCFAALSKSNTDQTLTSMAPNEFNVVATQ
                     DAKQFETKLSQQQITFSKNAYETITVDNVKDQVITLENGSDSGRTNSILSANNKVTGN
                     NAIITNTKSLPNIINIHLNKDLVVKGTKNETFRVTQEDKGRVYPLNLSFNSPVVEVSP
                     EKYQQLKTQNNVHTFYGYDIKQTSQKEKAQAIAKQFGDKVITYDEMKKEVDATNGILI
                     FVTSFLGLAFLVAAGCIIYIKQMDETEDELSNFRILKRIGFTHTDMLKGLLLKITFNF
                     GLPLLIAILHAVFAAIAFMKLMGNISFMPVIVVIVVYTLIYITFALIAFVHSNKLIKK
                     TI"
     gene            19506..19697
                     /gene="vraH"
                     /locus_tag="NBI57_000378"
     CDS             19506..19697
                     /gene="vraH"
                     /locus_tag="NBI57_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008530244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide resistance ABC transporter activity
                     modulator VraH"
                     /protein_id="HCD1821363.1"
                     /translation="MKFRELVKQSYEDLKNLTVTWYNVIALAMIVIVLSVVTTPIIGI
                     PAGLLGGAYYLKRREEKGK"
     gene            20089..20289
                     /locus_tag="NBI57_000379"
     CDS             20089..20289
                     /locus_tag="NBI57_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HCD1821364.1"
                     /translation="MNNGTVKWFNAEKGFGFIEQENGGDVFVHFSGIASDGYKTLEEG
                     QKVTFEITEGQRGDQAVNVQTV"
     gene            complement(20407..20976)
                     /locus_tag="NBI57_000380"
     CDS             complement(20407..20976)
                     /locus_tag="NBI57_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCD1821365.1"
                     /translation="MNLSKQIKKYRERDGYSQEYLAEKLYVSRQSISNWENDKSLPDI
                     HNLLMMCELFNVTLDDLVKGTIPFVPDIKVQRSLNLWTYVMLIFMTLAAILMGPLVVY
                     WNWTWGITVAIILGIGFYASMKIEDLKKVHKMDNYDRIVAFMNGKDPSEVQTTKARNT
                     MTNALSIISAIGILSLIIFLSVYMANKFL"
     gene            complement(21236..21631)
                     /locus_tag="NBI57_000381"
     CDS             complement(21236..21631)
                     /locus_tag="NBI57_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3147 family protein"
                     /protein_id="HCD1821366.1"
                     /translation="MKLTLMKFFVGGFAVLLSYIVSVTLPWKEFGGIFATFPAVFLVS
                     MFITGMQYGDKVAVHVSRGAVFGMTGVLVCILVTWMMLHMTHMWLISIIVGFLSWFIS
                     AVCIFEAVEFIAQKRLEKHSWKAGKSNSK"
     gene            complement(21699..22052)
                     /locus_tag="NBI57_000382"
     CDS             complement(21699..22052)
                     /locus_tag="NBI57_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3147 family protein"
                     /protein_id="HCD1821367.1"
                     /translation="MFSIGSAILHFVIGGIAVALASIIADKVGGKLGGIIATMPAVFL
                     AAIIALALDHRGTQLVEMSMNLSTGAIVGILSCILTVFLTSLYIKHKGYRKGAIFTVV
                     CWFVISLAIFSIRHL"
     gene            complement(22503..23342)
                     /gene="noc"
                     /locus_tag="NBI57_000383"
     CDS             complement(22503..23342)
                     /gene="noc"
                     /locus_tag="NBI57_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion protein"
                     /protein_id="HCD1821368.1"
                     /translation="MKKPFSKLFGLKNKDDIIGHIEEDRNSNVESIQIERIVPNRYQP
                     RQVFEPNKIKELAESIHEHGLLQPIVVRPIEEDMFEIIAGERRFRAIQSLNLPQADVI
                     IRDMDDEETAVVALIENIQRENLSVVEEAEAYKKLLEIGDTTQSELAKSLGKSQSFIA
                     NKLRLLKLAPKVLLRLREGKITERHARAVLSLSDSEQEALIEQVIAQKLNVKQTEDRV
                     RQKTGPEKVKAQNLRFAQDVTQARDEVGKSIQAIQQTGLHVEHKDKDHEDYYEIKIRI
                     YKR"
     gene            complement(23385..24104)
                     /gene="rsmG"
                     /locus_tag="NBI57_000384"
     CDS             complement(23385..24104)
                     /gene="rsmG"
                     /locus_tag="NBI57_000384"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HCD1821369.1"
                     /translation="MTVEWLAEQLKEHNIELTETQKQQFQTYYRLLVEWNEKMNLTSI
                     TDEHDVYLKHFYDSIAPSFYFDFNQPISICDVGAGAGFPSIPLKIMFPQLKVTIVDSL
                     NKRIQFLNHLASELQLQDVSFIHDRAETFGKGVYRESYDVVTARAVARLSVLSELCLP
                     LIKKGGQFVALKSSKGEEELEEAKFAISVLGGNVTETHTFKLPEDAGERQMFIIDKKR
                     QTPKKYPRKPGTPNKTPLLEK"
     gene            complement(24104..25981)
                     /gene="mnmG"
                     /locus_tag="NBI57_000385"
     CDS             complement(24104..25981)
                     /gene="mnmG"
                     /locus_tag="NBI57_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HCD1821370.1"
                     /translation="MVQEYDVIVIGAGHAGVEAGLASARRGAKTLMLTINLDNIAFMP
                     CNPSVGGPAKGIVVREIDALGGQMAKTIDKTHIQMRMLNTGKGPAVRALRAQADKVLY
                     QQEMKRVIEDEENLHIMQGMVDELIIEDNEVKGVRTNIGTEYLSKAVIITTGTFLRGE
                     IILGNMKYSSGPNHQLPSITLSDNLRELGFDIVRFKTGTPPRVNSKTIDYSKTEIQPG
                     DDVGRAFSFETTEYILDQLPCWLTYTNAETHKVIDDNLHLSAMYSGMIKGTGPRYCPS
                     IEDKFVRFNDKPRHQLFLEPEGRNTNEVYVQGLSTSLPEHVQRQMLETIPGLEKADMM
                     RAGYAIEYDAIVPTQLWPTLETKMIKNLYTAGQINGTSGYEEAAGQGLMAGINAAGKV
                     LNTGEKILSRSDAYIGVLIDDLVTKGTNEPYRLLTSRAEYRLLLRHDNADLRLTDMGY
                     ELGMISEERYARFNEKRQQIDAEIKRLSDIRIKPNEHTQAIIEQHGGSRLKDGILAID
                     LLRRPEMTYDIILEILEEEHQLNADVEEQVEIQTKYEGYINKSLQQVEKVKRMEEKKI
                     PEDLDYSKIDSLATEAREKLSEVKPLNIAQASRISGVNPADISILLIYLEQGKLQRVS
                     D"
     gene            complement(26048..27427)
                     /gene="mnmE"
                     /locus_tag="NBI57_000386"
     CDS             complement(26048..27427)
                     /gene="mnmE"
                     /locus_tag="NBI57_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HCD1821371.1"
                     /translation="MDLDTITSISTPMGEGAIGIVRLSGPQAVEIADKLYKGKHLLND
                     VPSHTINYGHIIDPESKEVVEEVMVSVLRAPKTFTREDIIEINCHGGILTINRVLELT
                     MTYGARMAEPGEFTKRAFLNGRIDLSQAEAVMDFIRSKTDRASKVAMNQIEGRLSDLI
                     KKQRQSILEILAQVEVNIDYPEYDDVEDATTEFLLEQSKEIKQEINRLLDTGAQGKIM
                     REGLSTVIVGKPNVGKSSMLNNLIQDNKAIVTEVAGTTRDVLEEYVNVRGVPLRLVDT
                     AGIRETEDIVEKIGVERSRKALSQADLILFVLNNNEALTQEDYTLYEVVKNEDVIVIV
                     NKMDLEQNIDINEVKDMIGDTPLIQTSMLKQEGIDELEIQIRDLFFGGEVQNQDMTYV
                     SNSRHISLLKQARQTIQDAIDAAESGVPMDMVQIDLTRTWEILGEIIGETASDELIDQ
                     LFSQFCLGK"
     gene            complement(27602..27955)
                     /gene="rnpA"
                     /locus_tag="NBI57_000387"
     CDS             complement(27602..27955)
                     /gene="rnpA"
                     /locus_tag="NBI57_000387"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HCD1821372.1"
                     /translation="MLLEKAYRIKKNADFQRIYKKGHSVANRQFVVYTCNNKEIDHFR
                     LGISVSKKLGNAVLRNKIKRAIRENFKVHKSHILAKDIIVIARQPAKDMTTLQIQNSL
                     EHVLKIAKVFNKKIK"
     gene            complement(28076..28213)
                     /gene="rpmH"
                     /locus_tag="NBI57_000388"
     CDS             complement(28076..28213)
                     /gene="rpmH"
                     /locus_tag="NBI57_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000240855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HCD1821373.1"
                     /translation="MVKRTYQPNKRKHSKVHGFRKRMSTKNGRKVLARRRRKGRKVLS
                     A"
     gene            28944..30305
                     /gene="dnaA"
                     /locus_tag="NBI57_000389"
     CDS             28944..30305
                     /gene="dnaA"
                     /locus_tag="NBI57_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HCD1821374.1"
                     /translation="MSEKEIWEKVLEIAQEKLSAVSYSTFLKDTELYTIKDGEAIVLS
                     SIPFNANWLNQQYAEIIQAILFDVVGYEVKPHFITTEELANYSNNETATPKETTKPST
                     ETTEDNHVLGREQFNAHNTFDTFVIGPGNRFPHAASLAVAEAPAKAYNPLFIYGGVGL
                     GKTHLMHAIGHHVLDNNPDAKVIYTSSEKFTNEFIKSIRDNEGEAFRERYRNIDVLLI
                     DDIQFIQNKVQTQEEFFYTFNELHQNNKQIVISSDRPPKEIAQLEDRLRSRFEWGLIV
                     DITPPDYETRMAILQKKIEEEKLDIPPEALNYIANQIQSNIRELEGALTRLLAYSQLL
                     GKPITTELTAEALKDIIQAPKSKKITIQDIQKIVGQYYNVRIEDFSAKKRTKSIAYPR
                     QIAMYLSRELTDFSLPKIGEEFGGRDHTTVIHAHEKISKDLKEDPIFKQEVENLEKEI
                     RNV"
     gene            30583..31716
                     /gene="dnaN"
                     /locus_tag="NBI57_000390"
     CDS             30583..31716
                     /gene="dnaN"
                     /locus_tag="NBI57_000390"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000969809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HCD1821375.1"
                     /translation="MMEFTIKRDYFITQLNDTLKAISPRTTLPILTGIKIDAKEHEVI
                     LTGSDSEISIEITIPKTVDGEDIVNISETGSVVLPGRFFVDIIKKLPGKDVKLSTNEQ
                     FQTLITSGHSEFNLSGLDPDQYPLLPQVSRDDAIQLSVKVLKNVIAQTNFAVSTSETR
                     PVLTGVNWLIQENELICTATDSHRLAVRKLQLEDVSENKNVIIPGKALAELNKIMSDN
                     EEDIDIFFASNQVLFKVGNVNFISRLLEGHYPDTTRLFPENYEIKLSIDNGEFYHAID
                     RASLLAREGGNNVIKLSTGDDVVELSSTSPEIGTVKEEVDANDVEGGSLKISFNSKYM
                     MDALKAIDNDEVEVEFFGTMKPFILKPKGDDSVTQLILPIRTY"
BASE COUNT        10621 a         5682 c         4825 g        10848 t
ORIGIN      
        1 ttgaaatatc gactattatt taaaaagaat aatgtaacta ctaacatcag aagtattaac
       61 gtcaaacaca aaacaacata atcatctttt tttaattgtt ttttgacgta ccaggttctc
      121 cttttatact gtccgaattg tcttaactcc atagcagtac taatagtgtc aattctttcg
      181 atactagaaa atattaaagg taacacaatg cgtttgatac ctttaacacg gctagtaaat
      241 ttcgcttttt tcgaattatc atatcctctt gcttgttgcg cttgtgaaat attaaaatat
      301 gtttcttgaa tatctggaat ataccttaat gctagtgaga ctgcgtaact gattttataa
      361 ttcacaccta gctgatttaa acttgcagca aaatgactcg gattcgttgt gaataaaaat
      421 attaacgcta aaggaactgt actaatatat tttaatatta gattaaaaag atagaataat
      481 tcctgtgatg ttagcgtaaa tcgaccaata ccattgacta actctgtacg ctgattataa
      541 atcttcacac catattcagg gtcaaatatg tatacggcaa taatatttaa tattgtgaaa
      601 aacagaatga atttaacaac aaagcgaact tgtttccatt caatatgagc atatttgaac
      661 aataaaatag atgaagcact aattaaaatt aaatatcttg tgtcataagt caccattgca
      721 gaaatagaaa caactaataa gaataaaagt ttaacagtcg catttaattg atgaatacga
      781 ctatttcccg ggtgaaaacc tatagtatta tattgattca ttggcgtctc accttcctat
      841 catggttaat aaataattgt actaatttct gtggctcgct aatccctata tattcggcca
      901 tttcaaatag cgatgtttgt ctcaatgatg caatctcaca gattttttta tcatttaata
      961 tcaatactgg cgtggtatca gcaacgactt gtccttttga taatacaact gttcttgaac
     1021 tatactcaga caataaatgc atatcatgcg taatcataat aatcgtcttt ccctgtctgt
     1081 ttagctcaat taaaaatgac attatctcat tataatgata gaaatcttga ccagcagtcg
     1141 gttcatccaa tatgattatt tccggattta agactaaaac agatgctata gtgaccctct
     1201 ttttttgacc atagcttaaa gctgcaattg gccaattccg aaatgcataa agtccgcata
     1261 ttttgagcac attctctact cgtatcttta tatctgattc tttcatacct cgtgctctta
     1321 accctaatgc tacttcatcg taaatcattt tttcagaaat catatgattt ggattttgca
     1381 tcacataacc tacgaattca gatcgttcgc taattgacaa ttgattaaat cccctattac
     1441 aaaattggat attacctgtt atatctaaaa atccacaaat tgctttagca agtgttgatt
     1501 tgccggcacc attgtgacca actatactaa gcatttctct tctataaaca tttaattgaa
     1561 cattattaag tacactatta ctatagtcac tatattgaac acatacctca tttaattcta
     1621 atagcggttc agatttgtac ttattatcat tatttgcaga tgtttcatct atccattttt
     1681 tcactttaaa tttaacatgt tcactcatac aaacgtcacg taaattcgct aagttatcaa
     1741 tggattcgac atctacttct gcatatttaa gcgctgtaca gtataatggt tcacgtatgc
     1801 ctgcttcttt aagcttagat gattttagca aatcactagg cgttgtatta gcgatgattt
     1861 ttccatcttt aaaaagaaga actctatcaa acgtatcatc taatgattct tctaatcgat
     1921 gttcgacaat aatcatcgtt gactttgttt cttcatgaat attgtttaac aatctcagcg
     1981 tttcatgtcc tgtcgcagga tctaaattgg ccagcggctc atccaatatt aaaataggcg
     2041 tacgatggat taatatacca cctaatgaaa cgcgttgttt ttgacctcca gataaatctt
     2101 gcggtcggtg atttaaatgt tctatcatgc caactttttc agcccaataa cttacatttt
     2161 tcttcatatc atcttgttca acacaattat tttctaataa aaaggccata tcttcagctg
     2221 ctgttaagcc tatgaactgt ccatctgtat cttgtaaaac tgtaccaaca acattagatc
     2281 tatcatgtaa acaactaacg gttgcatctt gattatttat atatagttcc ccggttatgt
     2341 tacctttagt tttaaatgga attaatccgt ttatgcaatt tgcaaaagtc gatttaccac
     2401 tacctgaagc accaactact agcacttttt ctcctggata aatatcaaca tttatattct
     2461 gtaatgtagg tgttgcttga ctatgatatt gaaaactaaa gtctttgaac gagataattg
     2521 gttcagtcat gatatatcat tacctttcta cattcattta catatctgat tcaacaaaat
     2581 gattattctt tacgtaaact accttttttt atttgagatg aagcatatgc ttttaataat
     2641 attgtcccaa taataccaac tgaaataata tttaatactg cagagataac accttgtgta
     2701 taaaccttgt tagccggttc gttataaatc aaaatatcta atgttggtgc aataagtgcc
     2761 cagcaaataa tattcgcaat aatttgaccg atattaaaat aaatcatcga tttcctagaa
     2821 aataggcctg aagaaagatt taattttagg ccaatccatc catataaaca gcctataatt
     2881 cctgaacaaa taacccaact ccaccaagca ctaccgtatg tcgtgaaatc tttaatagcg
     2941 tgtccaacta atccagtcat taaaccagca aaaggcccaa atattgcaga tattaatgct
     3001 aaaaatgcat aagatgtttc tatattcgta ttaggaaaac ctgttggtat tacaacaaaa
     3061 cgccctaaaa tcacaaatac cgctgctcct ataccaatcg caacaacagt tttaactgaa
     3121 atatcttgtt ttttcatctt cattactcct tacataaaaa attcattaaa ttgatggtgc
     3181 tttagataaa tgaatcgtcc aatcatttcc agtaccaata tgatataaat ctgaaaatga
     3241 gtcttgattg actgctacac caatatttac tagcgagtta acatacacaa gaggttcacc
     3301 cacattaaca tctgcaaacg atcgcgcaaa tttaataata ttttgataga ctttcttatc
     3361 ttgatgataa attgttacca ccaaattatt accatgaaca atttccaagg attttaagaa
     3421 tgccaatgga atatttgtcc ataatgaccc aaatctgata tctaaaatat caatgcttcc
     3481 tgtaacagaa tcctcatttt ttgtcgcttc tcttatttct aatgcctcaa tactatcaac
     3541 attaattgcc tgaccgagac gttcaaacgc tatcttattt gcagctaatc tcgcaccatt
     3601 gtatgcataa acatctctac catgaaaagt atgactttct tccgaatgag gcaatcggct
     3661 tttcacttca tcaatttcga taactttttt aataccttcg taatgtttaa tatgacttaa
     3721 agaaccatta tcaggtgtaa taatgtaatg acctgaatat gttaagcaag caatgctccg
     3781 cctatcacta cctacacccg ggtctaccac tgatacaaaa actgtgcctt taggccagta
     3841 ttttacagtt tgatataaac gatatgacgc tacccaaatg tcatacggtg gtatatcatg
     3901 cgttaagttt tcgacacgta tatcatcact tacagtatat gcaactccat acattgcact
     3961 tacagcgcca tcactaagtc caaaatctga ttgcaaaacc aaataatttc ccataaaatg
     4021 cctccttttt taaaacttca aaaaaatata atctacatat ttaacgttct atattttgaa
     4081 ttttcagaat tatattaaaa tagtacgagt cacttgatgc aaagtcaata gtagtgacta
     4141 taaaaatgat aataatagtc agatttatct cattaactaa atttcacgga cttgtaatca
     4201 cttctttatg tttgaattaa ttgactaggg tataacaata gtgtggtgtt ttataggtca
     4261 ttctttaaat acatcacaga aaatattcga attcgaaata aaatgcttgc atctttgttt
     4321 tttaggtggt attgtatgta tatcaagtat aaaaaatata tctaagagat aaaaatatgg
     4381 aggttttata ttatgactaa ctttactttt gatggtgcac acagtagttt agaattccaa
     4441 attaaacatt taatggtttc taaagtgaaa ggttcatttg atcaatttga tgtagctgtt
     4501 gaaggagata ttaatgactt cagtactttg aaagctactg caacaattat tccaagctca
     4561 attaacacta aaaacgaagc acgtgataac cacttaaaat ctggtgattt ctttggtact
     4621 gatgaatttg ataaaatcac gtttgaaaca aaatcagtta ctgaaaacaa agttgttggt
     4681 gatttaacaa ttaaaggtat cactaacgaa gaaacattcg acgttgaatt caacggagta
     4741 agtaagaatc ctatggatgg ttctcaagta acaggtgtta ttgttactgg tacaatcaat
     4801 agagaaaact acggcattaa ctttaaccaa gcacttgaaa ctggtggcgt aatgctaggc
     4861 aaagatgtta aattcgaagc atcagctgaa ttctcaatct cagaataatc tcattacaca
     4921 atcctcattg ttttaatata tatatacgaa atgccaacta tatcatccct aggtatagtt
     4981 ggcatttttc gtttaactca tgtgtaacaa gcactaactg ttcaccctaa tttaagacgc
     5041 ctttcattca ggtaggtctt attgaaactg aaaactttga tgaccttttg caaagccatt
     5101 aactgtataa agcatagatc ctccgcccat ttctatatca ttggaacaaa tgatgagttg
     5161 atttgttcca ggtataaatt gcggatgagt agaacgtaac atatgccctt tatctcggcc
     5221 tggtatcaat atttgtccta ttggataacc ccttttatta aaaactaaca ctcgaccttg
     5281 accatacatt gctacgtata aattatcgtc actatcaata caacatgagt ctggtccttc
     5341 atgacctgta aaatagtacg gtatagtagc tccaaatggt tgtatcgtca caccatcatc
     5401 ttcaagtgca atgcgatgta atcgattggc tgtagtttct gttacccata gtactttttc
     5461 atctgtactt aaagcaatac catttgctac gctaatattt tgaatgatag gcgtcactgt
     5521 tctaaagtcc ggcgaaacat aataaacgcc tcctagtgga ttggtagagt atcctctaaa
     5581 atctgtaaaa taaaatccac ctttagaatc aaataccatg tcatcaatac aatatgctgt
     5641 tgaaagatct tcaataatat cttgtaagtt gtcaccattt tctgtagctg caaaaatgcc
     5701 tcctgtagat ttaaaatctc ctaaataaca aacgaataat cggccatctt tatgtatttt
     5761 gattgctgca ggattcgctt tgtgacttac aaaaggtcgt ttgatttcct tcgtttcagg
     5821 attgattttg aaaatattgc cttcgaatac atccaataaa aagagttgtc cctgccgatc
     5881 aaagttcaat ccttctagtt gcaatccttt tttggaaatt tcaagccatg gttcagctgt
     5941 aattgtttgt aattcacttt cagatataat tggaacagca ctattggact tcccgctata
     6001 aaataatgta ggtaaatctt gttgtgacat catgatcatt cctttcattt cattcgtttt
     6061 aactatcaat tttattttaa acaagataac tatcaaataa aatgataacg gtttcatcta
     6121 tctattttat cggtctagtg gctgatttca agctagaaat attgaatgac aatacaactc
     6181 tgttaaaatg atggacgtag ataaatatgc gtattgacgc tttattttaa aaattaacat
     6241 gcttataaca tgtttataga aggagattaa cctatgaact atcaagttct tttatattat
     6301 aaatatacga cgattgatga ccctgaacag tttgctcagg atcacttagc gttttgcaaa
     6361 gcacaccatt taaaaggtag aattcttgtt tctacagaag gtattaacgg cacattatct
     6421 ggtacaaaag aagaaaccga acaatatatg gcacatatgc atgccgatga acgattcaaa
     6481 gatatggtgt ttaaaattga tgaagctgaa ggacatgctt ttaagaaaat gcatgtacgt
     6541 cctcgaaaag aaatcgttgc tttagattta gaagatgacg tcgatccaag acacacaact
     6601 ggccaatatt tatcacctgt agaatttaga aaagctcttg aagatgatga cacagtcatt
     6661 attgatgcac gtaatgatta tgaatttgat ttaggtcatt tccgaggtgc aattcgccca
     6721 gacatcacac gctttagaga tttacctgac tggattaaag agaataaagc gttatttaca
     6781 gataaaaaag tggttacgta ctgtacaggt ggcattcgat gtgaaaaatt ttctggatgg
     6841 cttttaaaag aaggtttcga agatgtagcg cagcttcacg gcggtatagc tacatatggt
     6901 aaagaccctg aaacaaaagg tcaatattgg gacggtaaaa tgtatgtatt tgatgatcgt
     6961 atcagtgttg atatcaacca agttgaaaaa acaattattg gtaaggattg gtttgatggc
     7021 aaaccatgtg aacgttatat taattgcgct aacccagaat gtaataaaca aatattagtt
     7081 tctgaagaaa acgaaactaa atatttaggt gcatgctctt atgaatgtgc taaacatgag
     7141 cgtaatcgtt atgttcaagc aaataatatt agtgataatg agtggcaaca acgtttaaca
     7201 aactttgatg atttacatca acatgcttag ttttaattaa atacctttca aaacacgctt
     7261 tgaaaatccg atttataaag gtttttcaag gctgtttttt atgcgcatac ataacctgat
     7321 atgtttttaa ttatgcgcgt gttatactaa attaaatttt aatactgcgg ggtgtcttaa
     7381 aatgcacatt ttagtaacag ggtttgcgcc ttttgacaat caaaatatca atccctcatg
     7441 ggaagctgtg actcaactag aagatattat tggcacacat acaatcgata aattaaaact
     7501 accaacctct tttaagaaag tagataatat tataaataaa acgttggcat ctaatcatta
     7561 tgatgttgta ctagctatag gacaagctgg tggtagaaat gccattaccc cagaacgtgt
     7621 cgccattaat attgatgatg cacgtattcc agataatgat gattttcaac ctattgatca
     7681 agccattcac ttagacggtg cgccagctta tttttcaaat ttaccagtta aagcaatgac
     7741 tcaaagtatt attaatcaag gacttcctgg agcactttca aatagcgcag gtacgtatgt
     7801 atgtaatcac gtgctttatc acttaggtta tttacaagat aagcattacc ctcacctacg
     7861 attcggattt attcatgtgc catacatacc agagcaggtc attggtaaac ccgatacacc
     7921 atctatgcca ttagaaaaga tagttgcagg tttgactgca gccattgaag ctatttctaa
     7981 tgatgaggat ttacgtatag ctctaggcac aacggaatag ttataaatgc acaaacaaaa
     8041 gcaccctaat ttgttagttt aagtctaact ttagggtgct tttggatgat tggtatacag
     8101 ttttactata atgatgtatt ttagctaaac gacattgttt agtcttcttt ttttaacttc
     8161 ctaaaattta caaatgtata aataataaat gctatattta gaatgatgag tatacttact
     8221 gaaactaaat taaaagtgtc tggttcttta ctaaagatag ctgctatcct tgctatcaaa
     8281 cttggcaatg aaattaccaa cacgatgagc gcataaattt tctgtttaat taaaatacaa
     8341 attacaataa gcgcaattaa aagtactaac ccaacgatga aactctgttt gtcacttaac
     8401 tcaaagaaac tatagatagg atatttttta ataatcaaac caccaaaaat catccataaa
     8461 aatacgacaa ttccataagt cacatttata acatacgtta ttttttggtc accaaatcgg
     8521 actaatgtat tgcgtagaat cagcatacca atgacaacac ctaaaataac gatactagct
     8581 atataaagta aaaatgcaat tgtcacatca aatgtaccca aatctaaaaa cctaggaatt
     8641 ataatgactg ctaatataaa agagaagtac aaagtaatat atttatacaa accggtagta
     8701 agacttatct caggtgataa ttgatcagcc attgacttaa tcggtgtatt aataattgaa
     8761 cttgtatctt cgttattttt ttcagccata gttaaatgat cttcgagctc ttccaataac
     8821 tcttctactt ctgcttcagt cttacctcta aataacaatt caacacgtaa tttttctaaa
     8881 aaatcttgag attgtttagt taacatcgtt ttcccctcca aacaagttaa tcatcccttt
     8941 attcaaagct tgccattttg atttaaatac ttttagttcc tttaaacctg aatcggtaat
     9001 cgtatagtat ttccgcctcg ggccgccatt actagatttt tttattgtcg tatcaacgta
     9061 tccttttttg tttaaacgca ttaaaactgg ataaatacta ccctcactta tctctggaaa
     9121 ctcttgattc ttaagtttcg tcataatttc atatccatac gtttcgcctt gggcaatgag
     9181 acctaatatc gccccatcta agagaccttt cataatctga tctgacactg acattttaat
     9241 cacctactat cttacataat aagatagtac attgagaact tttcgtcaac tatcttttat
     9301 tgtaaggtag ttgttgtaca cattccttaa atgactaaca actttgttaa tagggtaata
     9361 cttacggaag tatgttttat ttatggggga ggaattaata atgactacaa aaacagtatt
     9421 tgatgtcatt gatatggggt taggatattt agtaaatgtg tatgatgctt ggaaagttga
     9481 aaaggtactt gatgattatc atgagccttt ttctaatacc attcattggc aatttgggca
     9541 tgtattaaca atttttgaat cggccttagc tgttgctggt aaagagaata ttgatttaaa
     9601 tatctataga cctttattcg gaaatggttc gtctccagat gaatggaagg atgaagtacc
     9661 gagtattgaa aagattttag aaggtctcca aacattacct gaacgtgcac gaaatctaac
     9721 tgaagatgat ttagcaattg aattgaaaca accaattgtt ggttgtaata acttagaaga
     9781 gttattagta ttaaatgcca ttcacatccc acttcatgct ggtaaaattg aagagatgtc
     9841 tcgtatatta aaaaatttaa aataaatatg tgcttattaa ccgttaacaa cacgttaacg
     9901 ggttttttat ttgtttaaaa ggccactttt ttgaatttaa taaacaccat ctataccagt
     9961 tcttcaccgc ttctcgaaaa ataattatat taatgatttc gttaatttaa ttttatattt
    10021 aattattact gtacatcttt tgtagttagc tttattctta aattgaaata tgtataaaac
    10081 atagaaagaa ggacgtttta acatggaaaa cacggttaaa tatcgtaagt ttatactccc
    10141 tatcgttgta ggtctcctta tttgggcact tacacctttt aaaccggatg ctgtggatcc
    10201 aacagcatgg tatatgttcg caatattcgt cgcgacaatc attgcttgta ttacacaacc
    10261 gatgccaatt ggggccgtct ctataattgg atttacaatc atggtactcg ttggcattgt
    10321 tgacatgaaa acggctgtcg ctggttttgg taataatagc atttggttaa ttgctatggc
    10381 atttttcatt tcgagaggat ttgtgaaaac aggtcttggt agacggatcg cacttcattt
    10441 cgtcaaatta tttggtaaaa aaacattagg cttagcatat tctatcgtcg gtgtagattt
    10501 aattctagcg cctgctacac cgagtaatac cgcgcgtgct ggtggaatca tgttcccaat
    10561 tatcaaatca ctttctgaat catttggttc gaaaccgaaa gacggatcag cacgcaaaat
    10621 gggtgcattt cttgttttca cagaattcca aggtaattta attactgcgg ctatgttttt
    10681 aactgcaatg gccggtaacc cccttgcaca aaatttagca tctagcacat ctaatgttca
    10741 cattacatgg atgaattggt tcctagctgc tttagttcct ggacttgttt ccttaattgt
    10801 tgtacctttt attatttata aaatttatcc accaactgtt aaagaaacac caaatgctaa
    10861 gagttgggct gaaaatgaat tagcgactat gggtaaaatc gctttagctg aaaaatttat
    10921 gattggtatt tttgtcattg ctctaacatt atggattgtc ggaagtttca ttcatattga
    10981 tgcaacttta acggccttta ttgcgctagc attgttatta ttgacaggtg tcttaacatg
    11041 gcaagacatt ttaaacgaaa caggtgcttg gaacacatta gtatggttct cagtattagt
    11101 gttaatggcc gaccaattaa acaagcttgg atttattcct tggttaagta aatccattgc
    11161 tacaagtctt ggtggcttaa gctggcctat agtactggtc attttaatat tgttctactt
    11221 ctattcacat tacttatttg caagttctac agcacatatc agtgcgatgt atgcagcatt
    11281 actaggcgtt gccatcgcag ccggtgcacc accattattc agtgcattaa tgttaggttt
    11341 cttcggtaac ctattagctt caacaacaca ctatagtagt ggtccagcgc cgattctatt
    11401 ctcttcaggt tacgtgactc aaaaacgttg gtggacaatg aacttgatct taggtttcgt
    11461 ctactttatt atctggattg gtttaggatc actttggatg aaagtaattg gtatatttta
    11521 aaatatttaa attagtgctc gaatctcatt gatttgggcg ctttttaatt tgtatttaaa
    11581 atcaaccttt gctaaatcaa gactcccttt ttaaaatacg tttatccttt aaatcattgc
    11641 gtgcttcact gaaaatttgt ataaagattt aagtcattac gtaacatcac ataaaataca
    11701 tttctatact attccgtttc attgatcaac attacgtatg ccctcataaa tcatgaaata
    11761 aaaaaacacc ttcgtttaaa ttcattttaa gtgcgaattc aacgaaagtg atttatttca
    11821 tattcaatgt tttaaattta tacagctgtc actgttactg cacacatacc tcagtctcac
    11881 cttatcatga cacaaaggat attgaaaaca cgtataatta tctttcctcg tattttatag
    11941 ggtttttgcg accggatgtt tcttcaattt aatgtattga gaaagactat ataacacaat
    12001 acctgtccaa ataaatataa acgtaattaa ttgatctata ctaaaaggct ctttgaaaac
    12061 aaatatgccg agtacaaaca ttattgttgg tccaacgtat tgaataaatc ctattagcga
    12121 aagtggaata cgttttgccc cggctgagaa taggattagt ggtattgccg taatagcacc
    12181 agaaaataac aaccaaaatg atgacatgtt caatccaaat gacatctgat gttgctgcca
    12241 taaataaata acgtatatta gtccagcagg tgctgtaaca atacattcaa tcgtaatact
    12301 gctgatggca tcaatatgta ctactttttt caataatcca tatgtaccaa aggataacgc
    12361 taatataata gaaacgattg ggaattctcc aatcttgagc gtcatatata atacaccgat
    12421 gaatgcgaat aaaatggcta gccattcaaa tttattgaat ctttctttta aaaagataag
    12481 tgcgagcaaa atgctaacaa gtggatttat ataataacct aaacttgttt gtaggacgtg
    12541 accgttcgtt acagcccaaa taaatgtacc ccaatttaat gtaatgacat agcctgctac
    12601 gacaatcgct aatagctgaa tgggcttgcc taacaattga ttcatatctc gttgaaatgc
    12661 attgcgttgt ttttgtccaa ccgcgagtat gaatatcatg aatattgctg aaaatataat
    12721 acgaaaggct aaaatttcaa atgcgcctat tgcatcaacg aactgccaat atataggtag
    12781 tattccccac agaatgtatg cactgagtgc taaaaatata ccttttttat attctatatt
    12841 cacttacata cctccttact ctcccaaatt tttaacatgt tacgctcact tggttatgct
    12901 aatataacga ttttactaat aatatttcga taaagatatc attttgttta tatttcccac
    12961 atttattcac caaccactaa acaatattaa ttttataaat aattctgtac aaatcagggt
    13021 atattgccag aaagactacc atacaacata aaggatggat acaaatgact ttacctaaaa
    13081 ttggaaagcc tgcaacacgc gcgctaaatt cacaaggtat atacacatta gaagcagtat
    13141 cacaatatac gaagtcatct ctaatggaga tgcatggcgt tggtcctaaa gctatatcaa
    13201 tattggaaca agctttattt cagcaccaac tacattttaa aacggaagtt cagtcatcat
    13261 taccttttaa gttaacagga gatgtgtcat gcaatcatgc gcctaaacgt caacaaatga
    13321 tagattttat agttgctaca gcagcattag atattgaatt attacgttca ctggttacaa
    13381 cagaatttat ttggtctgtg cctggacact ttgacattta tggacctcaa attttaattc
    13441 aagaactatc taactattac aatcaggttg ctagtctcaa tatccactcc aacatcacac
    13501 atgggtgctt aggttccatg catggtattg aaatattaaa aactggtaaa gagattcatt
    13561 ttgctcattt ttttgaattt gaaaatcata aaaaggatgc caagttaagc aaagtgacat
    13621 cttacattgt tgttgcttaa tgactcataa tctataacgc taaaaactat cgtaattaca
    13681 tatatatatg ttaataaaac aaataatttt gcataatatt tttgttatat gaatgctttt
    13741 tactagagtg cacattgaac atacagtatg attaaatatc atgttatgaa agaaggatat
    13801 attttgactg tttttaaaaa tgagcgctta agctggttac catacataac tattgtcatt
    13861 ttgttacacg ttattgggtt tagtttttta tggattgctg gaaaagacca tcatatctta
    13921 tttggtatgg ggattcttgc atacacatta ggtttgcgtc atgcatttga tgcagatcat
    13981 atcgccgcga tagacaatac cgttcgtaaa ttattacaac aacgcaaaga tccatctggt
    14041 gtgggtttct atttttcaat tggacattca tctgttgtat ttttaatggc tgttttttta
    14101 gggatatctg taaaatgggc taaagatgaa ttaccacatt tccaagatat tggtggaacg
    14161 attggtacac tagtttcagg tttcttttta gtgcttatcg gtgtgttgaa tctaattatt
    14221 ttaatctctt taattaactt gttcgctaaa ttacgtcgtg aacacattga agaagctgaa
    14281 gtcgatgtat tacttgaatc tagaggattt gtttctcgat ttgtaggacc ttatttcaag
    14341 ttaatcacgc gtagttggca cgtattgcca cttggctttt tatttggact tggttttgat
    14401 acagctagtg aaattgcgtt actcgctctt tcttcaggcg catcacaaca agccatttca
    14461 tttatcggaa tcttatcttt accaatttta ttcgcatcag gtatgagttt attggataca
    14521 ttagatggtg ttgtaatgaa gtatgcctat aattgggcat tcttcaatcc tattcgcaaa
    14581 atctattaca atataacgat tactgcgata tcagtcatgg cagcattagt gattgggatg
    14641 attgaattgc tacaaattct tgctgataag ctagatttac atggtgcgtt ttgggcattc
    14701 attggttcga tcgaatttga ttatttaggc tatattttag ttgcattatt tttaattact
    14761 tggcttattt caagtttaat ttggaagttt ggtcgcatag aacacaaatg gtctaaataa
    14821 agtaacttgt cttgcaacta cctttgtatt gttgcatttt tagacaccct gcacacttct
    14881 tccattatgc tacgctctca gtagtcatct ttaaatcgtt tcttttagag atgacttcat
    14941 tttgagagcg tatttttcta tttaaaatgt atttatccaa aattcaatgc tttcgaattt
    15001 aaaatttcag catttctgtt taaaatttaa ttataatctt taatacagga ggcgctatgc
    15061 ttatgaatat tgcgaagtta gagaattatt tacaaattga ttcatctcgt tataatcgac
    15121 cgagtattga agcattaaat tattatgcaa cacgttttat gttaactgta ccttttgaaa
    15181 atattgatgt tcaaaatagt aagccgatat ctataaatat cgatgcactt tttaataaaa
    15241 ttgttcatga taaacgtggt ggtttttgtt atgagttaaa tacatttttc aaagcatatt
    15301 tacaacaaaa aggatttaat cctgaattaa tgtcagctac tattcacaca cccggtggcg
    15361 gtcgtagtct gaacggttca catgcttcac ttgttgtttc tataaatgat gtattctatg
    15421 taactgatgt tggctttgga gacttacctc tacatgccat tcctattact tcatctgagc
    15481 atacacaacc aattactgat atcagtggta cattccgcgc tatttttaac aacgaagaca
    15541 aggatatttt ctatgttcaa aaattcgaaa atgatcattg gcatacaaaa tatgaagctg
    15601 aattcaaacc taaacaaata gaagatttta atagcaacat cgaatataat caaacgcatc
    15661 cagattcaat atttgtacag catttattaa ttacaatgcc acaatcattt ggccgtgcta
    15721 caatgtctga aaatcattta actttaacaa ggaatggtag ctcagaaaaa tttgatgtca
    15781 ctaaagataa ctataaacat tttttagaaa aatattttgg actaaatgta actattaatc
    15841 gtattgaaaa acaataatag taaaatataa aaatatgggc gtatcttact tattgtaggt
    15901 agcgcccctt ttttatgctt cataaacaca acaaaaagga tatgacacaa acttcatgtc
    15961 acacccttct tttgaaccgc ttcattttac ttaagcaagt gcatctacaa cataaatgac
    16021 acctacgaat acaaatgcaa tagcaatcat cgtcgaaata aagacgatac ccattaatgg
    16081 attaaataat aaaatgacac cgaaaacaat acctaataaa ttaaaaatca ctgaaattag
    16141 ttttaagcta ccactacccg taaacgtaaa taatcctgca atagaactaa aaatgaacca
    16201 gaatgcaaac atgtagataa aaaatgccga gctagcgcca acattaaaaa tgacaataag
    16261 accaaagaga atatctacaa tccccataaa cagaatccaa ttttggttac cacctactaa
    16321 agcttttgct tttctacggt aaatgatttg aatcacaccg ttaattaata caaacagtcc
    16381 aatcaaccag gtaatagcat aaaaattttc aattggaaat gtaaaaataa cgactgccaa
    16441 cattaataat aatacaccca ttattaaact agaccattta ataccttgat ttcctttttc
    16501 cataacaatc aattcctttc aaataaagcc ctcttttaat ttttacaaaa aatttattat
    16561 acaataatta aattcccttt tctcaattta cacaaacatc atcacttaga aagcaccatt
    16621 gcttttgtct aatatgtacg attaatctgt atcctcactc aaaaatctac taagtagcga
    16681 tagctttcaa ctctgtaagg ttcattcatt gaattgtaag tttagataaa ggctactact
    16741 ccaaacatta tttaaaatga aattaaatac aaaaggagtg agactatgac aatattatca
    16801 gtgcaacatg tttcaaaaac atacggtaaa aagcacacat ttcaagcact taaagatatt
    16861 aactttgaca tacaaaaagg cgaattcgtt gcgattatgg ggccttctgg atcaggtaag
    16921 acaaccttat taaatgtact aagttcaatt gaccaaattt ctagcggtag cgtgattgct
    16981 aacggacaag agcttaataa acttaatcaa aaagcacttg ccaaattccg taaagaatca
    17041 ttaggtttca tcttccaaga ttacagtatt ctgccgacat taactgtcaa agaaaacatt
    17101 atgttacctt tatctgttca aaaaatgtcg aaagcaacaa tggaagaaaa ttataaagcg
    17161 attactacag cattaggtat ttatgaccta ggaaataaat accctagcga attatctggt
    17221 ggtcaacaac aaagaactgc agcagcgaga gcatttgttc acaaaccaca aatcatattt
    17281 gcagatgagc caacaggcgc actcgactcg aaaagtgcaa atgacctatt acaacgtttg
    17341 gaagaaatga ataaatcgtt tgatacaact attgtcatgg ttacacatga tccggttgca
    17401 gcaagttttg cagaacgtgt catcatgcta aaagatggcc aaattcatac acaactttat
    17461 caggaaggac gttctaaaca ggccttttat gaagacattg tacatcttca atcagtatta
    17521 ggtggtgtct caaatgacat ttaaccatat cgttttcaaa aacttacgac aaaacttaaa
    17581 acattatgca atgtatttat tttcattatt ttttagcatc gtcttatatt tcagttttac
    17641 aaccttacag tttactaaag gtgtaaataa tgacgactcg atggccatca ttaagaaagg
    17701 tgctttagtc ggatcaatat ttttatttat cattattgtc atctttttaa tgtatgccaa
    17761 tcatttattc gtgaaacgcc gtacacgtga atttgcgcta tttcagttga ttggtttaac
    17821 acgacaaaac attttgaaaa tgttagcact tgaacaaatg attgtatttt taatcacagg
    17881 tgttgtcggt gtattatgtg gcattgcagg tgcacaatta ttgctttcca tagtatctaa
    17941 attgatgtcg ttatcgatta acttatcgat acatttcgaa cctatggccc ttgttttaac
    18001 tattttcatg ctaattattg cgtatgtact gattttattt caaagtgctt tatttctaaa
    18061 aagacgtagt atcttatcaa tgatgaaaga ttcaattaaa actgatgcca caactgctaa
    18121 agtaacgact gcagaggtta tttcaggcgt attaggtatt gctatgattg cactaggcta
    18181 ttatatggcg acagaaatgt ttggaacatt taaagcacta actatggcta tgacatcacc
    18241 gtttatcatt ttatttttaa cggtagtagg cgcctattta ttcttcagaa gttccgtgtc
    18301 actaattttt aaaactttga aaaaatctaa aaatggacgc gtatctatta cagatgttgt
    18361 atttacatcg tctattatgt accgaatgaa gaaaaatgcg atgtctttaa ctatcattgc
    18421 aattatttct gcagttacag taactgtatt gtgtttcgcg gcattatcta aatcaaatac
    18481 agatcaaacc cttacatcta tggcaccaaa tgaatttaac gtggtcgcta ctcaagatgc
    18541 taaacaattt gaaactaaac taagccaaca acaaattaca ttttctaaaa atgcttacga
    18601 aactattact gtagataatg ttaaagatca ggttattact ttggaaaatg gtagtgattc
    18661 aggtcgcacg aattcaatat taagtgcaaa taacaaggta acaggcaaca atgccatcat
    18721 tacaaataca aaatcacttc ctaacattat taatatccat ttaaacaaag atttagtagt
    18781 aaaaggaact aagaatgaaa ctttccgtgt tacacaagaa gacaaaggta gggtttatcc
    18841 tttaaatcta agcttcaact cacctgtcgt cgaagtaagt cctgaaaagt atcagcagtt
    18901 gaaaacacaa aataacgttc atacttttta tggatatgat attaaacaaa catcacaaaa
    18961 ggaaaaagct caagcaattg caaaacagtt tggagacaaa gtcatcactt atgatgaaat
    19021 gaaaaaagag gtcgatgcta ctaacggtat tctaatattt gttacatcat ttttaggatt
    19081 agcattttta gtagcagctg gatgtattat ttatattaaa caaatggatg aaactgagga
    19141 tgaactaagt aacttcagaa tattaaaacg cataggcttt acgcatacag atatgcttaa
    19201 gggattatta ttaaaaatta ctttcaactt cggtttacca ttattgattg ctatattaca
    19261 tgcagtattc gccgcaatag catttatgaa attgatgggt aatatttcct tcatgccggt
    19321 tattgtagta attgttgtat acaccctcat ttacattact tttgctttaa ttgcttttgt
    19381 acattcaaac aaattgatta agaaaaccat ttaacactta cgattaaaag atgcttcatt
    19441 gagattttga ttttaagcaa tgcagtataa ctattaactg atttataaaa aggagagatt
    19501 gaatcatgaa atttagagaa ttagtaaaac aatcatacga ggatttgaaa aatttgactg
    19561 taacttggta taacgtgatt gctttagcaa tgattgttat tgtcttaagc gtagttacaa
    19621 caccaattat aggtattcca gctggtttat taggtggcgc ttattattta aaacgtcgtg
    19681 aagaaaaagg taaataagca cattccatcg gcatataaca ttgctatgac ctttcaagtc
    19741 agattttaat atcaacaagt agcatttatc gctgcttgtt ttttatgaat tgcttataaa
    19801 atcgtcatgt ttaaacgtta tgtcactttg aaaactagca aacaccataa aagactacaa
    19861 ttttttattt ttataaaccc ctttaaatca acgtttctaa ggtttttaaa aatcttttta
    19921 aatttatttt caaaaaacac tgtacattat gccaatatga gcgtatagtt ggtcttacgt
    19981 aataaaagct cgtgaattaa attgtagtgt atttgtttag aatatcctct tttttagtta
    20041 tgaatttgtt acaaatatta agtgcaaaag cacatggagg ttttctatat gaataacggt
    20101 acagtaaaat ggtttaacgc agaaaaaggt tttggtttca tcgaacaaga aaatggcgga
    20161 gacgtattcg tacatttctc aggtatcgct agcgatggct acaaaacttt agaagaaggt
    20221 caaaaagtta ctttcgaaat cactgaaggt caacgtggag accaagcagt taacgtacaa
    20281 actgtttaat cttacaaaat aaaacgactc attataaatg aatcgctttg attaccaaca
    20341 aggttctaaa tcttgttggt atttttttat gctattttgg ataatatgag acaatacgat
    20401 gatgatttat aaaaacttat ttgccatata cacactaagg aaaattatga ggctgagtat
    20461 accaattgct gatataatag aaagcgcatt tgtcatcgta tttctagctt tagtcgtttg
    20521 tacttcacta ggatcttttc cattcataaa agcaacaatt cgatcgtagt tgtccatttt
    20581 atgcactttt tttaaatctt ctattttcat agatgcataa aaacctattc ccaaaatgat
    20641 tgccaccgtt ataccccaag tccaattcca ataaacaact aaaggtccca ttaaaattgc
    20701 agctaatgtc atgaaaataa gcatcacata tgtccataag ttaagacttc gttgcacttt
    20761 aatatcaggt acaaatggaa tagtcccttt tactaaatca tctaaagtta cattgaacaa
    20821 ttcgcacatc attaataagt tatgtatgtc tggtaagctt ttgtcatttt cccaattaga
    20881 aatactctgc ctagatacat ataacttttc agcaagatat tcttgtgaat aaccatctcg
    20941 ttccctatac tttttaattt gtttactcag attcaattac atacgcctcc agtcaattag
    21001 tacaatcatt acaatcagac tatcagataa acttagttaa agtcaccttt atgattgact
    21061 aaatgttttg acatacgaca acatcggggt tcgaagactc ccttaagaca gacctgtata
    21121 attacgcgct tgtaattttc tcaatgccaa cttgaatttc acttattaat gatgcaactt
    21181 ttccatgcta ttcaatgctt gcaaagttag tcctagttaa gagattacgt tcacactatt
    21241 tactattcga ttttccagct ttccaactat gcttttctaa tcttttttgt gctataaatt
    21301 ctaccgcttc aaaaatacat actgcactga tgaaccagct taggaaacca acaataatgc
    21361 taatcaacca catgtgcgtc atatgtaaca tcatccatgt aactaaaata caaactaata
    21421 cccctgtcat accaaacact gcgccacgac ttacatgcac agcgacttta tcaccatatt
    21481 gcatacctgt aataaacata gacactaaaa atactgccgg aaacgttgca aatataccgc
    21541 caaattcttt ccaaggtagt gttacagata caatataact taataatact gcaaatcccc
    21601 ccacaaaaaa tttcattaat gttaatttca tgatgttctg cctccaaaaa tttattactt
    21661 aataactgaa tacaactaat tatcacgcat tttccaaact ataaatgtct aatactgaat
    21721 attgcgaggg aaataacaaa ccaacaaaca actgtgaata ttgcgccttt ccgataacct
    21781 ttatgcttaa tatagagaga tgtcaaaaat acagttaata tacaagacag aataccgaca
    21841 attgctccag tactaagatt catcgacatc tccactaatt gcgtaccacg atgatctaaa
    21901 gctaatgcga taatagccgc aagaaagact gccggcatag tagctataat acctcctaac
    21961 ttaccaccta ccttatcagc aataattgaa gctaatgcaa cagcgatacc accaatgaca
    22021 aaatgaagaa ttgcacttcc aattgaaaac atcttcaacg tacctttcta taattaattc
    22081 aatacaaaac gtatcattca cacaaagtgt aaaacttaaa ttttctactg taaaggcaac
    22141 tcaagcgatg ttcataaagc tttcatgata ctttcataaa ctagcaataa agggttcaaa
    22201 tgaaggtata tccagctcaa attagggagt gggacagaaa tgatattttc gcaaaattta
    22261 tttcgtcgtc ccaccccagc aaggttgact agaattgaaa aaagcttgtt acaagcgcat
    22321 tttcgttcag tcaactactg ccaatataac ttcgtagagc atagaacatt gatttatgta
    22381 ccagcctgat caacatataa atataaattt ttatgtttca cgtaaaacaa aaaaagacaa
    22441 agctgttatg atcttagctt tgtcttttat gtgttagtca tcatgtatac gacatcctac
    22501 tactaacgtt tatatattcg aatttttatt tcataataat cttcatgatc tttgtcttta
    22561 tgctcaacat gtagtcctgt ttgttgaatc gcttggatac ttttgcctac ctcatctcgt
    22621 gcttgagtga catcttgtgc aaagcgaagg ttttgtgctt tgactttttc gggccccgtt
    22681 ttttggcgta ctctatcttc agtctgtttc acatttagtt tttgtgcaat gacttgctca
    22741 atcaacgctt cttgttcact atcagacaat gataataccg ctctcgcatg gcgttcagta
    22801 attttacctt ctcttaagcg aagtagtact ttcggcgcca acttcaataa acgcaactta
    22861 tttgcaataa agctttgact tttacctaaa ctttttgcca attcactttg cgttgtatca
    22921 ccaatttcca ataatttctt ataggcttcc gcttcttcaa caacagacaa attttctctt
    22981 tgaatattct caattaatgc aacaacagcc gtctcttcat catccatatc acgaataata
    23041 acgtctgctt gaggtaaatt tagtgattgt attgctctaa atcggcgctc tccagcaata
    23101 atttcaaaca tatcttcttc aatcggtctt acaacaatag gttgtagtaa accatgttca
    23161 tgtattgatt cagcaagttc tttaatttta tttggttcaa acacctgtct tggttgataa
    23221 cggttgggaa cgatacgttc aatttgaatg gattcaacat tactattgcg atcttcttca
    23281 atatgtccaa tgatgtcatc tttgtttttc aaaccaaata attttgaaaa aggttttttc
    23341 attatccatt cgctcctttt atgttagttg taaaggatta tgcattattt ttcaagtaaa
    23401 ggagtcttat taggcgtccc tggttttctt gggtactttt tcggcgtctg tcttttttta
    23461 tcaataatga acatctggcg ctctccagca tcttctggca atttaaaggt atgtgtttct
    23521 gtaacgttac cacctaacac actaattgca aattttgctt cttctaattc ttcttcacct
    23581 tttgaagatt ttaatgcaac aaactgtcca ccttttttaa ttagcggtaa acacaattca
    23641 ctcaacacgg ataatctagc tactgctctt gcagtaacaa catcataaga ctccctgtag
    23701 acacccttac caaatgtttc tgctctatcg tgtataaagc tgacatcctg taattgtaat
    23761 tctgacgcta aatggtttaa aaattgaata cgcttattta atgaatcaac aatcgtcact
    23821 tttaactgcg gaaacattat ttttaacgga atacttggaa aaccagctcc agcgcctaca
    23881 tcacatatac ttataggctg attaaaatca aaataaaaac taggtgcaat ggaatcataa
    23941 aaatgtttca aatatacatc gtgttcatct gtaatacttg tcaaattcat cttttcattc
    24001 cattcaacaa gtaaacgata atatgtttga aactgttgtt tttgagtctc agttaattca
    24061 atattatgtt cttttaattg ttctgctaac cattctacag tcattagtca ctcacccttt
    24121 ggagtttacc ttgttctaag taaatcaata atatagatat gtctgctgga tttacccctg
    24181 atattctaga agcttgtgca atatttaaag gttttacttc tgataatttt tctcgcgctt
    24241 cagtcgccaa actatcaatc ttactataat ctaagtcttc tggaattttc ttctcttcca
    24301 tacgcttaac tttctcaact tgttgtagtg atttattgat ataaccttca tattttgttt
    24361 gtatttctac ttgttcttca acatctgcat tcaattgatg ttcttcttct aaaatttcta
    24421 aaattatatc gtaagtcatt tcaggtctgc gtaataaatc gatagctaaa ataccatctt
    24481 ttaagcgaga accaccatgt tgttcgataa tcgcttgcgt atgttcgttt ggtttaatac
    24541 gaatatctga taaacgctta atttccgcat caatttgctg acgtttttca ttaaaacgtg
    24601 catatcgttc ttcagaaatc ataccaagtt catatcccat atccgtcaat ctcaaatcag
    24661 cattatcatg acgtagtaac aaacgatatt ctgcacgtga tgttagtaaa cgataaggtt
    24721 cattagtacc tttagttaca agatcatcga ttaagacacc aatatatgca tctgaacgac
    24781 ttaatatctt ttcgcctgtg tttaacactt tacctgcagc gttaatacct gccatcaatc
    24841 cttgtcctgc tgcttcttca taaccagatg taccattaat ttgacctgca gtatataagt
    24901 ttttaatcat tttcgtttca agtgtaggcc ataactgcgt tggcacaatc gcatcatatt
    24961 caattgcgta gccggcacgc atcatatctg ctttttcaag acctggtatc gtctctaaca
    25021 tttgacgttg cacatgttca ggaagacttg tagacaatcc ttgcacatat acttcatttg
    25081 tattacgacc ttcaggctct aagaaaagtt gatgtcgcgg cttatcatta aatcgaacaa
    25141 atttatcttc aattgaaggg caataacgtg gcccggttcc tttaatcatc cctgaataca
    25201 ttgcagatag atgtaaatta tcatcgataa ctttgtgtgt ttcagcatta gtatacgtta
    25261 gccaacatgg caattgatct aatatatatt ctgttgtttc aaagctgaat gcacgaccta
    25321 catcgtcacc tggttgtatt tcagtcttcg aatagtcaat tgtttttgaa tttacacgcg
    25381 gtggtgtacc tgttttaaaa cgaacaatat caaaaccaag ttctcttaaa ttgtctgata
    25441 atgtgattga tggtaattgg tgatttggtc cacttgaata cttcatatta cctaaaatga
    25501 tttcaccacg taaaaatgtt cccgttgtaa taattactgc tttagataaa tactctgtac
    25561 caatatttgt acgtacacct ttaacttcat tatcttctat aataagttcg tctaccatac
    25621 cttgcattat atgcaaattt tcttcatctt caatcacgcg tttcatttct tgttgataaa
    25681 gtactttatc tgcttgcgct cttagtgctc ttacagcagg tcctttacct gtatttaaca
    25741 ttctcatttg aatgtgtgtt ttatcgattg tttttgccat ttgtccacct aaagcatcaa
    25801 tttcacgaac aacgatgcct ttagctggtc cacctacaga tgggttacat ggcataaatg
    25861 caatattatc taaatttatt gttagcatta atgttttagc accacgtctt gcagatgcta
    25921 aacctgcttc tacacctgca tgtcccgcac ctataacgat tacatcatat tcttgaacca
    25981 caatataaac ctccttattt gatatcttac tagccttctt aagacgatat tccgtctatt
    26041 tcaattacta tttacctaag cagaattgac tgaataactg atcgatgagt tcatcacttg
    26101 cagtctcacc aataatttct cctaatattt cccaagttct agttaaatca atttgtacca
    26161 tatccatagg cacaccagat tctgctgcat caatcgcatc ttgtatcgtt tgtcttgctt
    26221 gttttaataa tgaaatatgt cttgaattag aaacataagt catatcttga ttttgtactt
    26281 ctccaccaaa gaacaaatct cgaatttgta tttctaattc atcaatacct tcttgtttta
    26341 acattgaagt ttgaattaat ggcgtatcac ctatcatatc tttaacttca ttaatatcta
    26401 tgttttgctc taaatccatt ttattaacaa ttacgattac atcttcattt ttaaccactt
    26461 catataatgt gtaatcttct tgagtcaatg cttcgttatt gtttaataca aataaaatta
    26521 agtctgcttg gctaagagcc tttctagagc gttcaacacc aatcttctct actatatctt
    26581 ctgtctcgcg tataccagca gtatcaacta atcttaatgg cacgccacga acattgacgt
    26641 actcttctaa gacatctctt gtagtacctg ctacctcagt tacaatcgct ttattatctt
    26701 gtattaaatt atttaacatc gatgatttac ctacgtttgg tttaccaaca ataactgtag
    26761 ataaaccttc acgcataatt ttgccctgcg caccggtatc taataaacga ttaatttcct
    26821 gtttgatttc tttagactgc tctaaaagaa attcagtagt cgcatcttca acatcatcgt
    26881 attcaggata atcaatattc acttccactt gagcgagtat ctctaatata gattgacgtt
    26941 gttttttgat taagtcactt agacgacctt caatttgatt catcgcaact ttagaagctc
    27001 tatctgtctt cgagcgaata aagtccataa ctgcttcagc ttgagataaa tcaatacgac
    27061 catttaaaaa ggcacgtttt gtaaattcac ctggctcagc cattctagcg ccatatgtca
    27121 tagtaagttc cagcactcta ttaatcgtta aaataccacc atgacaatta atttctataa
    27181 tatcttcgcg tgtaaatgtt tttggcgctc ttaacacaga caccataact tcttcaacca
    27241 cttctttaga ctctggatca ataatatgac cgtaattaat cgtatgtgat ggaacatcat
    27301 ttaaaagatg ttttccttta tataatttgt cagcaatttc aacggcttgc ggtccagaca
    27361 atcgaacaat tccaattgcc ccttcaccca ttggtgttga aatactcgta attgtatcta
    27421 aatccatatt gctactcgcc tccttcaacg atgtgaatac attttaaagt aagttattat
    27481 aaccctaagg tcagtcttaa ccctaaggtc agtcttaacc ctaaggtcag tcttacgttt
    27541 gtctgaggta agacttcggg atgcgttgag tggttaatgt tttccttccc ctaccctatc
    27601 cttacttaat ctttttatta aaaactttgg caattttaag tacgtgctca agactattct
    27661 gtatttgtaa agtcgtcata tctttagctg gctgtcttgc tattacaata atatctttgg
    27721 ccaatatatg cgacttatgt actttgaaat tttcacgtat tgctctttta atcttgtttc
    27781 ttaacactgc attacctagt tttttagaaa cactaatacc taagcgaaaa tggtctattt
    27841 ctttattatt acaagtgtat acaacaaatt gtctgttggc tacagaatga ccttttttat
    27901 atattctctg aaaatctgca ttctttttaa ttcggtaagc tttttccaat aacatcactc
    27961 gcttatttat cgtttttatt tgaagctata tttaaacttc tattgagctt ataacataaa
    28021 tttctattta ttcttaattt aaacgaaaaa aaagatcact gatgagtcag tgatcttatg
    28081 cagataaaac tttacggcct ttacgacgac ggcgcgctaa aactttacgg ccattttttg
    28141 tgctcatgcg ttttctgaaa ccatgaactt tactatgttt acgtttattt ggttgataag
    28201 tacgttttac catgcaaaac acctccatct ttatacagtt atctaatata ccagattatg
    28261 cagactctga tcactttaat agataagatt ttgcgtttct tagattaaaa ttctcgtatt
    28321 agctcattga ttatctagtc ataattcaag caactactac aatataacaa aatccttttt
    28381 ataacgcaag ttcattttat actactgctc aattttttta cttttatcga ttaaagatag
    28441 aaatacacga tgcgagcaat caaatttcat aacatcacca tgagtttggt ccgaagcatg
    28501 agtgtttaca atgtttgaat accttataca gttcttatac atactttata aattatttcc
    28561 caagctgttt tgatacactc actaacagat attctataga aggaaaagtt atccacttat
    28621 gcacatttat agttttcaga attgtggata attagaaatt acacacaaag ttatactatt
    28681 tttagcaaca tattcacagg tatttgacat atagagaact gaaaaagtat aattgtgtgg
    28741 ataagtcgtc caactcatga ttttataagg atttatttat tgatatttac ataaaaatac
    28801 tgtgcataac taataagcag gataaagtta tccaccgatt gttattaact tgtggataat
    28861 tattaacatg gtgtgtttag aagttatcca cggctgttat ttttgtgtat aacttaaaaa
    28921 tttaagaaag atggagtaaa tttatgtcgg aaaaagaaat ttgggaaaaa gtgcttgaaa
    28981 ttgctcaaga aaaattatca gctgtaagtt actcaacttt cctaaaagat actgagcttt
    29041 acacgattaa agatggtgaa gctatcgtat tatcgagtat tccttttaat gcaaattggt
    29101 taaatcaaca atatgctgaa attatccaag caatcttatt tgatgttgta ggctatgaag
    29161 ttaaacctca ctttattact actgaagaat tagcaaatta tagtaataat gaaactgcta
    29221 ctccaaaaga aacaacaaaa ccttctactg aaacaactga ggataatcat gtgcttggta
    29281 gagagcaatt caatgcccat aacacatttg acacttttgt aatcggacct ggtaaccgct
    29341 ttccacatgc agcaagttta gctgtagccg aagcaccagc caaagcgtac aatccattat
    29401 ttatctatgg aggggttggt ttaggaaaaa cccatttaat gcatgccatt ggtcatcatg
    29461 ttttagataa taatccagat gccaaagtga tttacacatc aagtgaaaaa ttcacaaatg
    29521 aatttattaa atcaattcgt gataacgaag gtgaagcttt cagagaaaga tatcgtaata
    29581 tcgacgtctt attaatcgat gatattcagt tcatacaaaa taaagtacaa acacaagaag
    29641 aatttttcta tacttttaat gaattgcatc agaataacaa gcaaatagtt atttcgagtg
    29701 atcgaccacc aaaggaaatt gcacaattag aagaccgatt acgttcacgc tttgaatggg
    29761 ggctaattgt tgatattacg ccaccagatt atgaaactcg aatggcaatt ttgcagaaga
    29821 aaattgaaga agaaaaatta gatattccac cagaagcttt aaattatata gcaaatcaaa
    29881 ttcaatctaa tattcgtgaa ttagaaggtg cattaacacg tttacttgca tattcacaat
    29941 tattaggaaa accaattaca actgaattaa ctgctgaagc tttaaaagat atcattcaag
    30001 caccaaaatc taaaaagatt accatccaag atattcaaaa aattgtaggc cagtactata
    30061 atgttaggat tgaagatttc agtgcaaaaa aacgtacaaa gtcaattgca tatccgcgtc
    30121 aaatagctat gtacttgtct agagagctta cagatttctc attacctaaa attggtgaag
    30181 aatttggtgg gcgtgatcat acgaccgtca ttcatgctca tgaaaaaata tctaaagatt
    30241 taaaagaaga tcctattttt aaacaagaag tagagaatct tgaaaaagaa ataagaaatg
    30301 tataagtagg aaactttggg aaatgtaatc tgttatataa cagcactaat aataactgtc
    30361 attttttaca tttctatata ctaatgtgac aagatgagca aaactcattt tgtggataat
    30421 gtttaaaagt catacacacc atacacaagt tatcaacatg tgtataactt cgccaaatct
    30481 atgtttttac gacttatcca ccaatccaca gcacctacta ctattactaa gaacttaaaa
    30541 cctatataat tatatataaa cgactggaag gagttttaat taatgatgga attcactatt
    30601 aaaagagatt attttattac acaattaaat gacacattaa aagctatttc accaagaaca
    30661 acattaccta tattaactgg tatcaaaatc gatgcgaaag aacatgaagt tatactaact
    30721 ggttcagact ctgaaatttc aatagaaatc actattccta aaactgtaga tggcgaagat
    30781 attgtcaata tttcagaaac aggctcagta gtacttcctg gacgattctt tgttgatatt
    30841 ataaaaaaat tacctggtaa agatgttaaa ttatctacaa atgaacaatt ccagacatta
    30901 attacatcag gtcattctga atttaattta agtggcttag atccagatca atatccttta
    30961 ttacctcaag tttctagaga tgacgcaatt caattgtcgg taaaagtgct taaaaacgtg
    31021 attgcacaaa cgaattttgc agtgtccacc tcagaaacac gcccagtact aactggtgtg
    31081 aactggctta tacaagaaaa tgaattaata tgcacagcga ctgactcaca ccgcttggct
    31141 gtaagaaagt tgcagttaga agatgtttct gaaaacaaaa atgtcatcat tccaggtaag
    31201 gctttagctg aattaaataa aattatgtct gacaatgaag aagacattga tatcttcttt
    31261 gcttcaaacc aagttttatt taaagttgga aatgtgaact ttatttctcg attattagaa
    31321 ggacattatc ctgatacaac acgtttattc cctgaaaact atgaaattaa attaagtata
    31381 gacaatgggg agttttatca tgcgattgat cgtgcctctt tattagcacg tgaaggtggt
    31441 aataacgtta ttaaattaag tacaggtgat gacgttgttg aattatcttc tacatcacca
    31501 gaaattggta ctgtaaaaga agaagttgat gcaaacgatg ttgaaggtgg tagcctgaaa
    31561 atttcattca actctaaata tatgatggat gctttaaaag caatcgataa tgatgaggtt
    31621 gaagttgaat tcttcggtac aatgaaacca tttattctaa aaccaaaagg tgacgactcg
    31681 gtaacgcaat taattttacc aatcagaact tactaaaaat aaatataaat aaaggatgac
    31741 gtgattaatt aaaacgtcat cctttatttt ttggcaaaaa taattctaga tgcgtatgta
    31801 aaataaattt gacagcattt taaacagcaa ataaaagacg ccaattaaat ttatgacaaa
    31861 tgtatccaaa atttaataag tgtgcttata tgccctttaa atttaaaatt ttaatagtca
    31921 ataacaagtt gaatattaaa gttaaacgcc gttaaatagc gttaaaaaat tgaaaa
//
LOCUS       DAHHSC010000011        31142 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.011, whole genome shotgun sequence.
ACCESSION   DAHHSC010000011 DAHHSC010000000
VERSION     DAHHSC010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 31142)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31142)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31142
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.011"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            <1..334
                     /locus_tag="NBI57_000391"
     CDS             <1..334
                     /locus_tag="NBI57_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HCD1821376.1"
                     /translation="TGYIVLMAIENDEKLNIKKLGERVFLDSGTLTPLLKKLEKKDYV
                     VRTREEKDERNLQISLTEQGKAIKSPLAEISVKVFNEFNISEREASDIINNLRNFVSK
                     NFDYSDRK"
     gene            550..978
                     /locus_tag="NBI57_000392"
     CDS             550..978
                     /locus_tag="NBI57_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HCD1821377.1"
                     /translation="MILNFNQFENQNFFNGNPSDTFKDLGKQVFNYFSTPSFVTNIYE
                     TDELYYLEAELAGVNKEDISIDFNNNTLTIQATRSAKYKSEQLILDERNFESLMRQFD
                     FEAVDKQHITASFENGLLTITLPKIKPSNETTSSTSIPIS"
     gene            complement(1085..2254)
                     /locus_tag="NBI57_000393"
     CDS             complement(1085..2254)
                     /locus_tag="NBI57_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000278551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NarK/NasA family nitrate transporter"
                     /protein_id="HCD1821378.1"
                     /translation="MYKTKGGFQLTLQTLSLVVGFMAWSIIAPLMPFIKQDVNVTEGQ
                     ISIILAIPVILGSVLRVPFGYLTNIVGAKWVFFTSFIVLLFPIFFLSQAQTPGMLMAS
                     GFFLGVGGAIFSVGVTSVPKYFPKEKVGLANGIYGMGNIGTAVSSFLAPPIAGIIGWQ
                     TTVRSYLIIIALFALIMFIFGDTQERKIKVPLMAQMKTLSKNYKLYYLSYWYFITFGA
                     FVAFGIFLPNYLVNHFGIDKVDAGIRSGVFIALATFLRPIGGILGDKFNAVKVLMIDF
                     VIMIIGAVILGISDHIALFTVGCLTISICAGIGNGLIFKLVPSYFSNEAGSANGIVSM
                     MGGLGGFFPPLVITYVANLTGSSHLAFIFLAVFGCIALFTMRHLYQKEYGSLKHS"
     gene            2623..3537
                     /locus_tag="NBI57_000394"
     CDS             2623..3537
                     /locus_tag="NBI57_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000276756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2268 domain-containing protein"
                     /protein_id="HCD1821379.1"
                     /translation="MYKINIIRSDSVYKNILKAPINDRDSIFTKEILVPFKKKFEVQH
                     MPIYNDDKQTMSAIQFLDAFQISPKDLRMSDQMSIQYLNNDFWSNCEKYLKVAIDQFS
                     NYSISSQVSNYHFTVLLGDSQKPLMYLNKNRGGDGGIPGYIMIYLVPSTSTINSMKSL
                     IAHEVNHNMRYQYIDWDGGSLIELIIAEGLAENYVESLYGKAHIGPWVTNTNWSRDNV
                     KIKNTIYNHLHLKHIFESMPYLYGDDINKLQGRPIVGLSHAAGYACGYHLVKYFLQKT
                     NIPIEVATTLPAQKIINEVTEFWHTHTL"
     gene            3516..4256
                     /locus_tag="NBI57_000395"
     CDS             3516..4256
                     /locus_tag="NBI57_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HCD1821380.1"
                     /translation="MAYTYTLKDIIEITGVTKRTLHYYDEIGLLVPDKNDKNYRVYKQ
                     QDLEKLQKILILKSFDFDIAKIKQYISYDNEQLRKLLSEQISKLDKKISDLQLIRRSV
                     CEFIKGLSLIDTSILNKTLQSQYDKEASIKYGHTKAYQSFIRRKDSLQSQDIRHKLTT
                     IFNKFNHMSLSHYPIQDCSDLVFEWKAFMNTIADFDDETLCCIAKTYEDDTRFKDYFN
                     SYDNQNLASYISEAVNYFLSNVNKSDNF"
     gene            complement(4514..5167)
                     /gene="nreC"
                     /locus_tag="NBI57_000396"
     CDS             complement(4514..5167)
                     /gene="nreC"
                     /locus_tag="NBI57_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002464689.1"
                     /note="Involved in the regulation of the the nitrate
                     reductase operon narGHJI; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate respiration regulation response
                     regulator NreC"
                     /protein_id="HCD1821381.1"
                     /translation="MKIVIADDHAVVRTGFSMILNYQNDMEVVATAADGVEAYQKVME
                     YKPDVLLMDLSMPPGESGLIATSKIADSFPETKILILTMFDDEEYLFHVLRNGAKGYI
                     LKNAPDEQLLLAIRTVYKGETYVDMKLTTSLVNEFVSNSNQDTANTTDPFKILSKREL
                     EILPLIAKGYGNKEIAEKLFVSVKTVEAHKTHIMTKLGLKSKPELVEYALKKKLLEF"
     gene            complement(5190..6224)
                     /gene="nreB"
                     /locus_tag="NBI57_000397"
     CDS             complement(5190..6224)
                     /gene="nreB"
                     /locus_tag="NBI57_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501138.1"
                     /note="Involved in the regulation of the the nitrate
                     reductase operon narGHJI; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate respiration regulation sensor histidine
                     kinase NreB"
                     /protein_id="HCD1821382.1"
                     /translation="MINEDSIQLDTLLKKYYEHSIEKIVFADDNGKIIAMNDAAKDIL
                     SEEDNYSAVANAICHRCEGYTNAYDVQSCKDCFLESMQVQATNFQVFMKTKDQKVMPF
                     TATYQLIDQDRGIHAFTLQNVSSQIEQQEKLHQQRMMRKTISAQENERKRISRELHDS
                     VIQEMLNVDVQLRLLKYQEDTTKLLEDAENIEYIVAKLIDDIRNMSVELRPASLDDLG
                     LEAAFKSYFKQFEENYGIKIIYTSNIKNTRFDSDIETVVYRVVQEAILNALKYADVNE
                     INVGIRQTGRHLVAEVIDAGNGFDPSSKPKGSGLGLYGMNERAELVSGSVNIETKIGE
                     GTNVTLNIPI"
     gene            complement(6248..6700)
                     /gene="nreA"
                     /locus_tag="NBI57_000398"
     CDS             complement(6248..6700)
                     /gene="nreA"
                     /locus_tag="NBI57_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate respiration regulation accessory nitrate
                     sensor NreA"
                     /protein_id="HCD1821383.1"
                     /translation="MTPEAIIEDRRFQETLDKIRKEEGYDFAAIAFYESNKPSSPIKW
                     HYVSGNKNNRFKLIILRKGRGLAGTVMKTGKRMVIANVGLALGPEEKIDYPILLSESL
                     TAVLAVPLWYKNQVYGVLLFGQRDGRPLPKIFDNDDIQRKFGIFNDDK"
     gene            complement(6720..7397)
                     /gene="narI"
                     /locus_tag="NBI57_000399"
     CDS             complement(6720..7397)
                     /gene="narI"
                     /locus_tag="NBI57_000399"
                     /EC_number="1.7.99.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HCD1821384.1"
                     /translation="MLNQFLWVIFPYLCLVVFVAGHIARYRYDKFSWTAKSSELIERK
                     RLMWGSLLFHLGIIPVFFGHVVGLLIPKSWMDAVGVSEHLYHIGAVYIGSIFGIITLI
                     GMFLLTARRVTTKSIRRLSSASDIFVNFLLLLIVFMGCYATLVTNIQHPDFNYRTSLS
                     IWFRQLFMLKPDASLMSGVPIAFKMHILLGFTIMACWPFTRLVHVWSVPLTYINRRYI
                     IYRKNKV"
     gene            complement(7390..7965)
                     /gene="narJ"
                     /locus_tag="NBI57_000400"
     CDS             complement(7390..7965)
                     /gene="narJ"
                     /locus_tag="NBI57_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000606770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HCD1821385.1"
                     /translation="MINFDNFKKYQESFGYMAQQLCFPEKLTFHPKTFEETISKDHPA
                     YEDLVAFRNVMMTFSLSEIKAIYTDTFDFTKKAPLYMTYNKFDTQKERGQMLAKLKVL
                     YEMFGLEMVDNELSDYLPLMLQFLQVAEWRNDPRAEENIQLIIMIIEDGTYVMANALG
                     EDNNPYAYVIQALRKTLKLCLTSPKGVKHHA"
     gene            complement(7958..9517)
                     /gene="narH"
                     /locus_tag="NBI57_000401"
     CDS             complement(7958..9517)
                     /gene="narH"
                     /locus_tag="NBI57_000401"
                     /EC_number="1.7.99.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009384084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HCD1821386.1"
                     /translation="MKIKAQVAMVLNLDKCIGCHTCSVTCKNTWTNRPGAEYMWFNNV
                     ETKPGVGYPKRWEDQEHYKGGWVLNRKGKLELKSGSRISKIALGKIFYNPDMPLIKDY
                     YEPWNYNYEHLTTAKSGKHSPVARAYSEITGDNIEIEWGPNWEDDLAGGHVTGPKDPN
                     IQKIEEDIKFQFDETFMMYLPRLCEHCLNPSCVASCPSGAMYKRDEDGIVLVDQDACR
                     GWRYCMTGCPYKKVYFNWKTNKAEKCTFCFPRIEAGMPTVCSETCTGRMRYLGVLLYD
                     ADRVHEAASAVDEKGLYEKQLDIFLNPFDEEVIAQAEKDGIGYDWIEAAQNSPIYKLA
                     IEYKLAFPLHPEFRTMPMVWYCPPLSPIMSYFEGKNTTQNPDAIFPAIEEMRLPIEYL
                     ANIFTAGDTEPVKGALQRMAMMRSYMRSQVTQQPFDTSRLERLGITERQTKDMYRLLG
                     LAKYEDRFVIPTSHKETYLDTYHAQGSTGYNYGGEHFGDNCEGCGVAVGSGKTGQEIY
                     NENFYGGIFRD"
     gene            complement(9507..13196)
                     /locus_tag="NBI57_000402"
     CDS             complement(9507..13196)
                     /locus_tag="NBI57_000402"
                     /EC_number="1.7.99.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit alpha"
                     /protein_id="HCD1821387.1"
                     /translation="MGKFGLNFFKPTEKFNGNWSILESKSREWEKMYRERWSHDKEVR
                     TTHGVNCTGSCSWKVFVKNGVITWENQQTDYPSCGPDMPEYEPRGCPRGASFSWYEYS
                     PLRIKYPYIRGKLWDLWTEALEENYGNRVAAWASIVENEDKAKQYKQARGMGGHVRSN
                     WKDVTEIIAAQLLYTIKKYGPDRIAGFTPIPAMSMISYAAGARFINLLGGEMLSFYDW
                     YADLPPASPQIWGEQTDVPESSDWYNASYIIMWGSNVPLTRTPDAHFMTEVRYKGTKV
                     ISVAPDYAENVKFADNWLAPNPGSDAAIAQAMTHVILQEHYVNQPNERFINYAKQYTD
                     MPFLIMLDEDENGYKAGRFLRASDLGQTTEQGEWKPVIHDAISDSLVVPNGTMGQRWE
                     EGKKWNLKLETEDGSKINPTLSMAEGGYELETIQFPYFDSDGDGIFNRPIPTRQVTLA
                     NGDKVRIATIFDLMASQYGVRRFDHKLESKGYDDAESKYTPAWQEAISGVKQSVVIQV
                     AKEFAQNAIDTEGRSMIIMGAGINHWFNSDTIYRSILNLVMLCGCQGVNGGGWAHYVG
                     QEKCRPIEGWSTVAFAKDWQGPPRLQNGTSWFYFATDQWKYEESNVDRLKSPLAKTED
                     LKHQHPADYNVLAARLGWLPSYPQFNKNSLLFAEEAKDEGIESNEAILKRAINEVKSK
                     QTQFAIEDPDLKKNHPKSLFIWRSNLISSSAKGQEYFMKHLLGTKSGLLATPNEDEKP
                     EEITWREETTGKLDLVVSLDFRMTATPLYSDIVLPAATWYEKHDLSSTDMHPYVHPFN
                     PAIDPLWESRSDWDIYKTLAKAFSEMAKDYLPGTFKDVVTTPLSHDTKQEISTPYGVV
                     KDWSKGEIEAVPGRTMPNFAIVERDYTKIYDKYVTLGPVLEKGKVGAHGVSFGVSEQY
                     EELKSMLGTWSDTNDDSVRANRPRIDTARNVADAILSISSATNGKLSQKSYEDLEEQT
                     GMPLKDISSERAAEKISFLNITSQPREVIPTAVFPGSNKQGRRYSPFTTNIERLVPFR
                     TLTGRQSYYVDHEVFQQFGESLPVYKPTLPPMVFGNRDKKIKGGTDALVLRYLTPHGK
                     WNIHSMYQDNKHMLTLFRGGPTVWISNEDAEKHDIQDNDWLEVYNRNGVVTARAVISH
                     RMPKGTMFMYHAQDKHIQTPGSEITDTRGGSHNAPTRIHLKPTQLVGGYAQISYHFNY
                     YGPIGNQRDLYVAVRKMKEVNWLED"
     gene            complement(13505..14482)
                     /gene="cobA"
                     /locus_tag="NBI57_000403"
     CDS             complement(13505..14482)
                     /gene="cobA"
                     /locus_tag="NBI57_000403"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HCD1821388.1"
                     /translation="MSVDKYGKVYLIGAGPGNPNYLTKKAERLIREADVILYDRLVNP
                     LILQYANLTTEIIDVGKKPYAKHIQQEKINECIVEAARRYNKVVRLKGGDPAIFGRVQ
                     EEVDTLNNFNIAFEIVPGVTSASAAVATMQTGLTMRAVAKSVTFSTGHFKDSEENEVD
                     VNALVNGGTLAIYMGVKRLGKIIAQIQQYTDIDYPIAIVFQASCFNEFVVKGRLSNIV
                     GKLEHYAIEAKPGICIIGEVVGYTENVSTTSNPTQQFYVVSGSRHDALMLCEHLYDEG
                     YGCLLNPNDTSNGTYHSSQYDYYDAFIKQQENVTYISTDRADANTVLCH"
     gene            complement(14473..14787)
                     /gene="nirD"
                     /locus_tag="NBI57_000404"
     CDS             complement(14473..14787)
                     /gene="nirD"
                     /locus_tag="NBI57_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase small subunit NirD"
                     /protein_id="HCD1821389.1"
                     /translation="METKEKIKVTTIDELTPLIGKKVIVKGKEVGLFLTESGTIHAIH
                     NICPHKQGPLSEGTVSGEYVFCPLHDQKIDLNTGIVQEPDEGCVDVYEVEVTDGNVYI
                     CL"
     gene            complement(14791..17196)
                     /locus_tag="NBI57_000405"
     CDS             complement(14791..17196)
                     /locus_tag="NBI57_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HCD1821390.1"
                     /translation="MAKQKLVMIGNGMAGIRTIEEILERANDLYDITVIGKEPYPNYN
                     RIMLSNILQNKMTVEETIMNPYEWYEEHGIELITNDPVIEVDRANQSVTTANGIEVSY
                     DKLIFATGSKAFVIPVPGSTLPSVIGWRTIDDTEQMMNIAKTKKKAIVIGGGLLGLEC
                     ARGLLDQGMEVTVLHLAEWLMEMQLDRKAGNMLKADLEKQGMKFEMQANTTEILGEDD
                     VEGVKLADGREIPADLVVMAVGIRPYTEVAKESGLDVNRGIVVNDVMQTSDSNVYAVG
                     ECAEHNGKVYGLVAPLYEQGKVLADHLTNKETNGYKGSTTFTSLKVSGCDLYSAGQIV
                     ENAEIKGIEIFNSVDNNYKKIFLKDGNVVGAVLYGDIDDGSRFYNMMKKGESTEDYTL
                     VSLLTKGGEEASLSIADMADDETICGCNGVDKGTIVNAITENGFTTVEEVTAKTKAGN
                     SCGKCKPQIAQILQHTLGDDFVAAKPAGICGCTDLTRDQIVTQIRAKGLKTSKEVRHV
                     LNFKNKGGCPKCRPAINYYLNMVYPHDHEDERESRFANERYHANIQNDGTFSVIPQMR
                     GGVTDADQLIRLGEVAKKYHVPLVKVTGSQRVGLYGVKKEELPNIWEDLGMRSASAYG
                     KKTRSVKSCVGKEFCRFGTQYTTRLGIRLEKTFEYIDTPHKFKMGVSGCPRSCVESGV
                     KDFGIISVENGFQIYIGGNGGTEVEKAEFLTTVETEDEVIKLCGALMQYYRETGIYAE
                     RTAPWLRRLGFENVKEVLLDPERQNELFERIMDAKKAVEAEPWEAITSNAQARKIFEV
                     EKV"
     gene            complement(17262..17993)
                     /locus_tag="NBI57_000406"
     CDS             complement(17262..17993)
                     /locus_tag="NBI57_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sirohydrochlorin chelatase"
                     /protein_id="HCD1821391.1"
                     /translation="MNGNIIVAHGMRHGRQNQALEAFISELVKDDIHHYDIAFLESEH
                     QDLETVMTTLIQSGVDHFKIVPLLIFSAMHYLKDIPNIVHEMKRRYPDIKVEVSEPLG
                     THPLMRRLIEQRICDALAGDEQQVGVMVVAHGNINGNFTKAHEELQICAQHLQINKPT
                     YARTLYGEISFTHDLESISKRYQKLIVVPLFLYDGRLVNKVKQQMNDMVINTDIHFTP
                     SINFDPILKQIINDRLESLMIPMKI"
     gene            complement(18223..18759)
                     /locus_tag="NBI57_000407"
     CDS             complement(18223..18759)
                     /locus_tag="NBI57_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCD1821392.1"
                     /translation="MTKTMIRLASEQDAEKLQQLMHEAFTPLRELGIDWPSVNANLDA
                     VKENIDKNTTFVMTIDDEIISTITVRYPWGSVKSISGYPFVWWFATNPEYEGKGYGSQ
                     LLTYVEEAFLRDTLKSAAVTLGTSARLHPWLLKIYEKRGYEIYSEHENDDGDLGVIMR
                     KVLIPERFDETILGQPPF"
     gene            complement(18993..19817)
                     /locus_tag="NBI57_000408"
     CDS             complement(18993..19817)
                     /locus_tag="NBI57_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HCD1821393.1"
                     /translation="MIENKKTVEDTYSTGAIVDSISSSVQMKQVMVQQTPGRYMLKAM
                     MAGFLLSIVTVFMFGIKTQFANTHVDGLINLMGAIAFSLGLILVVLTNSELLTSNFMY
                     FTVGWYYKVVSVKKMTWILLYCFLGNILGGFVLFFLMKFAHVMTPEMTQALTALVQKK
                     TVDSTWLNIFTKGIFCNFFINIGIFISMQFKGGLTKAFFIACGVVVFVYMGYEHVVFN
                     AGLYAGMVFFNLDAVSWLHVLKNIVFAFLGNFVGGGIFVGLVYAFLNGKRNRLEQQ"
     gene            complement(20034..20216)
                     /locus_tag="NBI57_000409"
     CDS             complement(20034..20216)
                     /locus_tag="NBI57_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1821394.1"
                     /translation="MVERYIKVLILYIFTTLLSSISVTSKCVPNKVIRFILRTAIGYS
                     IFAYGLHYFSNLKKNK"
     gene            complement(20300..20767)
                     /locus_tag="NBI57_000410"
     CDS             complement(20300..20767)
                     /locus_tag="NBI57_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HCD1821395.1"
                     /translation="MTIKVEDNKIIFSRTIEAPIEKVFDAYTTKELFEKWFHPKGAST
                     KVFRFNAVSGGDAFYAIKTPTMTSYTLAEYKTVKRPYLIEYIDSFATPQGAKDTKMPS
                     MKITLSFSKSNTTKTTVTSTSVFPTKEAAQQVINMGVEQGMNQTLDQLDALLK"
     gene            complement(20953..22500)
                     /gene="adcA"
                     /locus_tag="NBI57_000411"
     CDS             complement(20953..22500)
                     /gene="adcA"
                     /locus_tag="NBI57_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding
                     lipoprotein AdcA"
                     /protein_id="HCD1821396.1"
                     /translation="MKKKLGMLLLVPAVTLSLAACGNDDGKDKDGKVTIKTTVYPLQS
                     FAEQIGGKHVKVSSIYPAGTDLHSYEPTQKDILSASKSDLFMYTGDNLDPVAKKVAST
                     IKDKDKKRSLEDKLDKAKLLTDQHEHGEEHEHEGHDHEKEEHHHHGGYDPHVWLDPKI
                     NQTFAKEIKDELVKKDPKHKDDYEKNYKKLNDDLKKIDNDMKQVTKDKQGNAVFISHE
                     SIGYLADRYGFVQKGIQNMNAEDPSQKELTKIVKEIRDSNAKYILYEDNVANKVTETI
                     RKETDAKPLKFYNMESLNKEQQKKDNITYQSLMKSNIENIGKALDSGVKVKDDKAESK
                     HDKAISDGYFKDEQVKDRELSDYAGEWQSVYPYLKDGTLDEVMEHKAENDPKKSAKDL
                     KAYYDKGYKTDITNIDIKGNEITFTKDGKKHTGKYEYNGKKTLKYPKGNRGVRFMFKL
                     VDGNDKDLPKFIQFSDHNIAPKKAEHFHIFMGNDNDALLKEMDNWPTYYPSKLNKDQI
                     KEEMLAH"
     gene            complement(22689..22760)
                     /locus_tag="NBI57_000412"
                     /pseudo
     CDS             complement(22689..22760)
                     /locus_tag="NBI57_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501155.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(22795..23061)
                     /locus_tag="NBI57_000413"
     CDS             complement(22795..23061)
                     /locus_tag="NBI57_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HCD1821397.1"
                     /translation="MARLNITFSPQAFEDYKYFQQNNKKMVKKINELLKSIDRNGALE
                     GIGKPEKLKSNLTGYYSRRINHEHRLVYTVDDNHIKIASCKYHY"
     gene            complement(23061..23312)
                     /locus_tag="NBI57_000414"
     CDS             complement(23061..23312)
                     /locus_tag="NBI57_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HCD1821398.1"
                     /translation="MIIKNYSYARQNLKALMTKVNDDSDMVTVTSTDDKNVVIMSESD
                     YNSMMETLYLQQNPNNAEHLAQSIADLERGKTITKDIDV"
     gene            complement(23477..23578)
                     /locus_tag="NBI57_000415"
                     /pseudo
     CDS             complement(23477..23578)
                     /locus_tag="NBI57_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501155.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(23585..24184)
                     /locus_tag="NBI57_000416"
     CDS             complement(23585..24184)
                     /locus_tag="NBI57_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HCD1821399.1"
                     /translation="MTKKLLTLFIVSMLILTACGKKESATTSSKNGKPLVVIYGDYKC
                     PYCKELDEKVMPKLRKNYIDNHKVEYQFVNLAFLGKDSIVGSRASHAVLMYAPKSFLD
                     FQKQLFAAQQDENKEWLTKELLDKHIKQLHLDKETENKIIKDYKTKDSKSWKAAEKDK
                     KIAKDNHIKTTPTAFINGEKVEDPYDYESYEKLLKDKIK"
     gene            complement(24203..24565)
                     /locus_tag="NBI57_000417"
     CDS             complement(24203..24565)
                     /locus_tag="NBI57_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000819849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4467 domain-containing protein"
                     /protein_id="HCD1821400.1"
                     /translation="MKRFVATVLLLLVFISGCGNDKYVKEIDEAVKIQNQKQEQLAKK
                     GNGDRVDHFERKDANIYVYDKDKIIILAYKPLSNDDEVHYYAYDFSDKRVSYKQDFDS
                     RRYYQQHDADYHEENMTN"
     gene            24820..26070
                     /gene="fmhA"
                     /locus_tag="NBI57_000418"
     CDS             24820..26070
                     /gene="fmhA"
                     /locus_tag="NBI57_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FemA/FemB family glycyltransferase FmhA"
                     /protein_id="HCD1821401.1"
                     /translation="MNFVTLTSDEFNAFTTKHFSHYTQSAIHYNHRVDLKGDVHLVGV
                     KDDNGQVIAGCLLTEARTLKFFKYFYTHRGPVMDYTNQSLVAFFFKALTSYLKKHNCL
                     YVLVDPYLIENLRNADGEIVKSYDNRAFVRTMDKLGYKHQGFPVGYDSMSQIRWLSVL
                     DLKDKTEDQLLKEMDYQTRRNIKKTYDIGVKTKTLTIDETQTFFDLFHMAEEKHGFKF
                     RELPYFEEMQKLYDDHAMLKLAYIDLNEYLKTLQLKQQQLTAELSGVEEALEESPNSK
                     KNKTKRTQLEQQLNSNKRKIDNTIEQIEQDGAVLNLASALFIYNEHEVYYLSSGSNPK
                     YNAYMGAYHLQWEMIKFAKAHHIDRYNFYGITGDYSESSEDYGVQQFKKGFNAHVEEY
                     IGDFIKPIKPLLYKVQTYLNHKRR"
     gene            complement(26167..26898)
                     /locus_tag="NBI57_000419"
     CDS             complement(26167..26898)
                     /locus_tag="NBI57_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HCD1821402.1"
                     /translation="MIQLNNIHKSFNDVEVIKGIDLSVEQGEVVTLIGRSGSGKTTLL
                     RMINALEIPTEGTVYVNGKTYTSKDKKSQIEVRKQSGMVFQSYNLFPHKTALENVMEG
                     LITVKKLKKDEARGKSLELLEKVGLTHVKDQRPHALSGGQQQRVAIARALAMNPKVML
                     FDEPTSALDPELVNDVLKVIKDLANEGMTMVIVTHEMRFAKEVSNNIVFIHEGMIGEQ
                     GAPEEMFNRPKTEELRRFLNVINEE"
     gene            complement(26895..27614)
                     /locus_tag="NBI57_000420"
     CDS             complement(26895..27614)
                     /locus_tag="NBI57_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HCD1821403.1"
                     /translation="MFLNLNSEQQHALDAAKQAFGPMLEGLVKYSIPITLVTFVLGLI
                     IALFTALMRISTSKILRSISRVYVSIIRGTPMIVQLFIIFYGIPELGRLLTNDADNQW
                     TLAPVVAAIIGLSLNVGAYASEIIRGGIISIPKGQTEAAYSIGMTYGQTIQRIILPQA
                     IRVSIPALGNTFLSLIKDTSLLGFILVAEMFRKAQEVASTTYEYLTIYVLVALMYWVV
                     CFIISIIQGIYESYIERGYRS"
     gene            complement(27595..28374)
                     /locus_tag="NBI57_000421"
     CDS             complement(27595..28374)
                     /locus_tag="NBI57_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HCD1821404.1"
                     /translation="MKRLLFVMIAFVFILAACGNNSSKDKEANKDSKTINVGTEGTYA
                     PFSFHDKDGKLTGYDIDVIKAVAKEEGLKLKFNETSWDSMFAGLDAGRFDVIANQVGI
                     NPDREKKYKFSKPYTFSSAVLVIRENEKDIKDFDDVKGKKLAQTFTSNYGKLAKDKGA
                     DITKVDGFNQSMDLLLSKRVDGTFNDSLSYLDYKKQKPNAKIKAIKGNAEQSRSAFAF
                     SKKADDETVQKFNDGLKKIEENGELAKIGKKWFGQDVSKSK"
     gene            complement(28495..29934)
                     /gene="mdeA"
                     /locus_tag="NBI57_000422"
     CDS             complement(28495..29934)
                     /gene="mdeA"
                     /locus_tag="NBI57_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdeA"
                     /protein_id="HCD1821405.1"
                     /translation="MSKKQKLTMIITMLMGGFFGLLNETLLVTALPSIMKDFEISYTQ
                     VQWLTTAFLLTNGIVIPLSALVIQRYTTRQVFLVGISIFFLGTLLGGLSPHFATLLVA
                     RIIQALGAGIMMPLMMTTILDVFQPHERGKYMGIFGLVIGLAPAIGPTLSGYLVEYLN
                     WRSLFHVVAPIAAVTFLIGFKTIKNVGTTIKVPIDFISVIFSVLGFGGLLYGTSSISE
                     KGFDNPIVLVSMIGGVVLVALFVLRQYRLSTPLLNFAVFKNKQFTVGIIIMGVTMVSM
                     IGSETILPIFVQNLLHRSALDSGLTLLPGAIVMAFMSMTSGALYEKFGPRKLALVGMA
                     IVVITTAYFVVMDEQTSTIMLATVYAIRMVGIALGLIPVMTHTMNQLKPEMNAHGSSM
                     TNTVQQIAGSIGTAALITILSHASKNFSPTMSDYNGMNKIDMMNQIKVDTMLHGYHAG
                     FLFALLITVVSFFCSFMLQGKKKEVDSRQ"
     gene            30108..30212
                     /locus_tag="NBI57_000423"
     CDS             30108..30212
                     /locus_tag="NBI57_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HCD1821406.1"
                     /translation="MKLDLQTARRNLNSPNIKTRKRALKIIKQHKRAK"
     gene            complement(30366..31052)
                     /locus_tag="NBI57_000424"
     CDS             complement(30366..31052)
                     /locus_tag="NBI57_000424"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HCD1821407.1"
                     /translation="MPKLILCRHGQSEWNAKNLFTGWEDVNLSEQGINEATRAGEKVR
                     ENNIAIDVAFTSLLTRALDTTHYILTESKQQWIPVYKSWRLNERHYGGLQGLNKDDAR
                     KEFGEEQVHIWRRSYDVKPPAETEEQREAYLADRRYNHLDKRMMPYSESLKDTLVRVI
                     PFWTDHISQYLLDGQTVLVSAHGNSIRALIKYLEDVSDEDIINYEIKTGAPLVYELTD
                     DLEVIDKYYL"
BASE COUNT         9870 a         6024 c         4419 g        10829 t
ORIGIN      
        1 tactggttac attgttttaa tggcgattga aaatgatgaa aaacttaaca tcaaaaaatt
       61 aggtgaacgt gtattcttag attctggaac actgacacca ttactaaaga aattagaaaa
      121 gaaagattac gttgttcgaa cacgtgaaga gaaagatgaa agaaacctac aaatttctct
      181 aacagaacaa ggtaaagcaa taaaaagccc tcttgctgaa atttctgtta aggtctttaa
      241 tgaattcaac atttcagaac gagaagcatc agatataatt aataatttgc ggaattttgt
      301 ttctaaaaat tttgactatt ccgacagaaa gtaactccct atcataaggt gatgggcaga
      361 agtatgcatc gctatgttta ttcattatct ttattgcttg tataaatctc gatgctcagt
      421 tcttttgccc tgattcctta tcaggcacaa atctacccat ttataacttt tttatctccc
      481 cttttacttt atctttttta atactactca tataatggct ttgtaataca aatttctata
      541 atggaagtga tgatattgaa cttcaatcaa ttcgagaatc aaaacttttt taacggtaat
      601 ccaagtgata catttaaaga tttaggtaaa caagtattta attacttttc aacaccttca
      661 tttgtaacga atatatatga aacagacgaa ttatattact tagaagctga actagcaggt
      721 gtaaataaag aagatattag tatcgatttc aataataata cgctcactat tcaagctact
      781 agaagcgcaa aatacaaatc tgaacaactc attttagatg agcgtaactt cgaatcatta
      841 atgcgtcaat ttgattttga agctgttgat aagcaacata ttactgctag ttttgaaaat
      901 gggttattaa ccattacatt gcctaaaatc aaaccaagca atgaaactac ttcatcaaca
      961 tctattccaa tttcatagtt aaatttaaaa tgaatacact actaaagcct taatgtcatg
     1021 ctacgaatat tgtgcatgat attaaggctt tgtatttaac taaatgaatg tcatgtatta
     1081 catatcaact gtgtttcaat gaaccatatt ctttttgata taaatggcgc atggtaaata
     1141 atgcaatgca tccaaatacc gctaagaaaa taaatgctaa atggcttgaa cctgttaaat
     1201 tagctacgta tgtgatgacc aacggtggga agaatcctcc taaaccaccc atcattgata
     1261 cgataccatt tgctgatcct gcttcatttg agaagtagga tggtactaat ttaaaaatta
     1321 agccgttacc aatacctgca caaatactta ttgttaaaca gcctacagtg aataatgcga
     1381 tatggtctga aatacctaaa ataacggcac cgataatcat aataacaaaa tcaatcatca
     1441 gtactttaac tgcattaaat ttatcaccta gtataccacc tattggtctc aagaatgttg
     1501 ccagcgcaat gaatacacct gaacgaatac cagcatctac tttgtcaatt ccaaaatgat
     1561 ttactaagta gttaggtaag aaaataccaa atgctacaaa agcaccaaaa gtaatgaaat
     1621 accaataact taagtaatat aatttgtagt ttttagataa tgttttcatt tgggccatta
     1681 atggcacttt aattttacgt tcttgtgtgt caccaaaaat gaacataatt aatgcaaata
     1741 aagcgataat aattaagtaa cttctaactg ttgtttgcca accaataata cccgctatcg
     1801 gtggtgctaa aaatgaagaa actgctgtac cgatattccc cataccataa ataccatttg
     1861 ctagacctac tttttctttt gggaaatatt taggaactga tgtaacacca acagagaaaa
     1921 ttgcgccacc tacgccaagg aaaaatcctg atgccattaa catacccggc gtttgtgctt
     1981 ggcttaagaa aaatatcggg aataacaata cgataaaact tgtaaagaat acccatttag
     2041 caccaacaat atttgttaaa taaccaaatg gcacacggag cactgatccc aaaataactg
     2101 gtatcgctaa aatgattgat atttgacctt cagtaacatt gacatcttgt ttaataaagg
     2161 gcattaatgg cgcaattata ctccaagcca taaacccaac cactaaactt aatgtttgta
     2221 atgtaagttg aaagccacct tttgttttgt acatacgttt caccttcatc aaaatcattt
     2281 attttttaat agatatcaat actctgccta tatattagag gtcaatgtta tttaaaaatg
     2341 ctttcattta tatgaaaaca ttcaatatag acaaagtatt gactacatat atattttatt
     2401 atgattgtga aaaagtgaac ataaggattt tcccttattt atgaagtgat ttaccctata
     2461 caatgtccct ttaagatgct tttagacact tattcgccat cattttaatc atattattta
     2521 ggaacactca catacttttc ttattttttt caattagtta caattactgc ttgaaaatca
     2581 cgttacgtta tcctttatgc tgacaacata aggaggttta tcatgtataa aatcaatatt
     2641 attagatcag attccgtcta taagaatata ctcaaggcac caataaacga ccgtgattca
     2701 attttcacta aagaaatatt agtacctttt aaaaagaaat ttgaggtaca acatatgcct
     2761 atttataatg atgacaaaca gacaatgagt gcgattcaat ttttagatgc ctttcaaatt
     2821 tctcccaaag atttacgcat gtctgaccaa atgtcaatac aatacctgaa taatgatttc
     2881 tggagtaatt gcgaaaaata tttaaaagtt gctatagatc aattttcaaa ttatagtatc
     2941 tcatctcaag tttctaacta tcatttcaca gtattacttg gagatagcca aaaaccactt
     3001 atgtatctaa ataaaaatcg cggtggtgat ggtggcatac caggttatat tatgatttat
     3061 ttagtgccga gtacaagtac aattaattct atgaaaagct taattgcaca tgaagtaaat
     3121 cataatatgc gctatcaata tattgattgg gatggcggaa gtttgattga attgattatt
     3181 gcagaaggat tagctgaaaa ctatgtagaa tcattgtatg gtaaagcgca tattggaccg
     3241 tgggtaacaa atactaattg gagtcgtgat aacgtaaaaa ttaaaaatac tatttataat
     3301 catttacatt taaagcatat atttgaatcg atgccttatc tctatggtga tgatattaat
     3361 aaacttcaag gtaggcctat cgttggccta tctcatgctg ccgggtatgc atgtggctat
     3421 cacttggtaa aatacttttt acaaaaaaca aacataccta ttgaagttgc tacaacactt
     3481 ccagcacaaa aaataataaa tgaggtgact gaattttggc atacacatac actttaaaag
     3541 atattattga aattacaggt gtaactaaaa gaactttaca ttattacgat gaaataggat
     3601 tattagttcc agataaaaat gataaaaatt atcgcgttta taaacagcaa gacttagaaa
     3661 aattacaaaa gattttaata ctcaagtctt ttgattttga tatcgctaaa ataaaacaat
     3721 acatttcgta tgataatgaa caattgcgaa agttattatc agagcaaata agcaagttag
     3781 ataaaaagat ttctgactta caattaatta gacgctctgt atgtgaattt attaaaggac
     3841 tctctctaat agataccagc attttaaaca agacactaca gtcacaatat gataaagaag
     3901 catctataaa atatggtcat acgaaagcat atcaatcatt tattagacgt aaagacagct
     3961 tacaatcgca ggatatcaga cataaattga caactatctt caataaattt aatcatatgt
     4021 ctttgagtca ttatccaatc caagattgta gtgatctcgt atttgagtgg aaggcattta
     4081 tgaacactat cgctgatttt gatgatgaaa cattatgctg tattgctaaa acatatgaag
     4141 atgatacgcg tttcaaagat tactttaatt catatgataa tcaaaattta gcatcataca
     4201 tttcagaagc tgttaattat tttttgagca atgtgaataa gagcgacaat ttttaatcct
     4261 catacagatg ctacaacact gaatacacca actaaaataa ttgttcaaag tagaaaacta
     4321 atcatttatt ctctaaatca ataactataa taaaagttat acctttgcaa agcgaattag
     4381 tataggttac cgaaaggaga aaggattagg ttccattcga tttataaaaa aatatatttc
     4441 gaaacataca aaagcgccag aagataatca tttcctctcg cgcttttata ctgaattata
     4501 ttaatttcaa actctaaaac tctaataatt tctttttcaa tgcatattca actagctcag
     4561 gtttactctt taagccaagc tttgtcataa tatgcgtctt atgtgcttct actgttttca
     4621 cagatacaaa taatttctct gcaatttctt tattcccgta acctttggca ataagtggca
     4681 atatttctag ttctcgtttt gataagattt taaaaggatc tgttgtgttt gcagtgtcct
     4741 gatttgaatt agatacaaat tcattcacta aagatgttgt aagtttcata tctacatatg
     4801 tttcaccttt atatacagtt cgaatagcta acaataattg ttcatcaggt gcatttttca
     4861 atatgtaacc tttcgcacca ttacgcaaca catggaacaa atactcctca tcatcaaaca
     4921 ttgttaatat tagtatttta gtttcaggaa aactgtcagc aattttactc gtagcgataa
     4981 gacctgactc acctggtggc atacttaaat ccattagtaa cacatcaggt ttatattcca
     5041 ttactttttg gtaagcttcg acgccatctg cagccgttgc aacaacttcc atatcatttt
     5101 gataatttaa aatcatagag aaccccgtac ggacaacagc gtgatcatcg gcaatgacta
     5161 ttttcaattt tattccccca atgtatgttt caaattggaa tgttcaatgt aacattggta
     5221 ccctcaccaa ttttcgtttc aatattgacg ctaccgctga ctaactcagc tcgctcattc
     5281 attccatata aaccgagtcc agaaccttta ggcttagaac ttggatcaaa accatttccc
     5341 gcatctatca cttctgctac caaatggcgc ccagtttgac ggatacctac atttatttca
     5401 tttacatcag cgtatttcaa cgcatttaga atagcttctt gcactactcg ataaacaacc
     5461 gtttcaatat cactatcaaa gcgagtattt ttaatatttg atgtatatat gatttttatt
     5521 ccataatttt cttcaaactg tttaaaatat gatttaaaag ctgcttcaag gcctagatca
     5581 tccaaagaag cgggtcttaa ttcaaccgac atattacgta tatcatcaat taatttagcg
     5641 acaatatatt caatattttc tgcgtcttcc aaaagcttag ttgtatcttc ttgatatttt
     5701 aataatctca attgaacatc tacattgagc atttcttgaa tcacactatc atgtaactct
     5761 ctagaaattc gctttctttc attttcttgg gctgagattg ttttacgcat catacgttgt
     5821 tgatgtaatt tctcttgctg ttcaatttgt gatgaaacat tttgaagcgt aaatgcatga
     5881 attcccctgt cttgatcaat caactgatat gttgctgtaa atggcatcac tttttgatct
     5941 ttcgtcttca taaatacttg gaaattcgta gcttgtactt gcatcgattc taagaaacag
     6001 tctttacatg attgtacatc ataggcattt gtataccctt cacatctgtg acaaatcgca
     6061 ttcgccacag cactgtaatt atcttcttca gataatatat ctttagcagc atcattcatt
     6121 gcaataattt tgccgttatc atcagcaaaa actatctttt cgattgaatg ctcataatat
     6181 tttttcaata aagtatctaa ctgtatactg tcctcattaa tcatgactta caccctaatt
     6241 catctcatta tttatcatca ttgaaaatac caaacttacg ttgaatatca tcattatcaa
     6301 atatttttgg taaaggacga ccatctcttt gaccaaataa tagtacgcca tacacttgat
     6361 tcttatacca aagcggcact gctaaaactg ctgttaatga ttcgctcaat aaaattggat
     6421 agtcaatctt ttcttcaggc cctaaagcta aaccaacatt ggctattacc atacgctttc
     6481 ctgttttcat aacagttcca gctaatccac gaccttttct taaaataatc aatttaaatc
     6541 gattattttt attacctgaa acatagtgcc attttattgg agatgatggt ttgttagatt
     6601 catagaaagc gattgccgca aaatcataac cctcttcttt gcgtatttta tctaatgtct
     6661 cttgaaatct acgatcttca attattgctt ctggtgtcaa atcctttcac ctcttatgct
     6721 tacactttat tcttacggta aataatatat ctgcgattta tatatgtcaa aggtacactc
     6781 caaacatgta ccaaacgtgt aaatggccaa caagccataa tagtgaaacc taacaatata
     6841 tgcattttaa atgcaatcgg cacaccactc atcaatgacg catctggttt taacataaat
     6901 aattgtctaa accaaattga taatgaagtt ctgtagttaa aatctggatg ttgtatattg
     6961 gttactaatg ttgcgtaaca tcccataaat acgataagta ataataagaa atttacaaat
     7021 atatccgacg ctgaacttaa tcttcgaata cttttcgtag taacacgtct cgctgttaat
     7081 aaaaacatcc ctatcaaagt tattatacca aagatgctac caatataaac agcgcctata
     7141 tgatataaat gctcagatac ccccactgca tccatccatg atttcggtat taacaatcca
     7201 actacgtgtc caaaaaacac tggaataata cctaagtgaa ataataaact tccccacatc
     7261 aacctttttc tttctattag ttcactagat ttagctgtcc aagaaaattt atcataacga
     7321 taacgtgcaa tatgacctgc gacaaagaca actaaacata aatacggaaa tataacccat
     7381 aaaaactgat taagcatgat gtttcactcc ttttggtgat gtcaaacata atttcaatgt
     7441 ttttctaagt gcttgaatca cataggcata tggattatta tcttcaccaa gtgcattcgc
     7501 catcacatat gttccatcct caataatcat aatgattaat tgaatattct cttcagctct
     7561 tggatcattt cgccattctg ccacttgcaa aaattgaagc atcaacggta gataatcaga
     7621 aagttcatta tctaccattt ctagtccaaa catttcatat aataccttta atttagctaa
     7681 catttgccca cgttcttttt gcgtatcaaa tttgttatac gtcatatata atggtgcttt
     7741 tttcgtaaaa tcaaatgtat ctgtataaat cgctttgatt tctgataatg aaaatgtcat
     7801 cattacattt ctaaatgcta ctaaatcttc atatgctgga tgatctttcg aaattgtttc
     7861 ttcaaaagtt tttggatgaa aagttaattt ttctggaaaa cataactgtt gtgccatata
     7921 tccaaaactt tcttgatatt ttttaaaatt atcgaaatta atcacggaaa atccctccat
     7981 agaaattctc attataaatt tcttgaccag ttttccctga acctactgca acgccacagc
     8041 cttcacagtt atctccaaaa tgctcgccgc cgtaattgta tcctgtacta ccttgtgcgt
     8101 gatacgtatc taaataggtt tctttgtgtg atgttggaat aacaaatcga tcttcatatt
     8161 tagctagtcc taataaacga tacatgtctt tagtttggcg ctcggttata cctaatcgct
     8221 ctaatcgaga cgtgtcaaat ggctgttgag taacttgaga tctcatataa cttctcatca
     8281 ttgccatacg ttgtagggct ccttttactg gctctgtatc tcctgcagtg aaaatattag
     8341 ctaagtattc aataggtaaa cgcatttctt caatggctgg gaaaatcgca tctggatttt
     8401 gagttgtatt tttaccttca aaatagctca taattgggct aagtggtggg caataccaaa
     8461 ccatcggcat cgttctaaat tcaggatgta acggaaatgc aagtttatat tcaattgcta
     8521 acttataaat tggagagttt tgtgcagctt caatccaatc ataaccaata ccatcttttt
     8581 cagcttgagc aatgacttct tcgtcaaatg gatttaagaa tatatctaat tgtttttcgt
     8641 ataaaccttt ctcgtctact gctgaagccg cttcatgaac tcgatctgca tcatataata
     8701 aaacacctaa gtaacgcata cgtcctgtac aagtttcaga gcataccgta ggcatacccg
     8761 cctcgattct cgggaaacag aaagtacatt tttcagcttt gttcgttttc caattgaagt
     8821 aaactttctt atatggacaa cctgtcatac agtaacgcca tccacgacat gcgtcttgat
     8881 caactaatac aatgccatct tcatcacgtt tatacatagc acctgaaggg cacgatgcaa
     8941 cgcaacttgg attcaagcaa tgttcacata aacgtggtaa atacatcata aaagtttcgt
     9001 caaattggaa tttaatatct tcttctattt tttggatgtt aggatctttt gggcctgtaa
     9061 catgaccacc tgctaagtca tcttcccagt taggtcccca ttcaatttca atgttatccc
     9121 ctgtaatttc tgaatacgct ctagcaactg gcgaatgctt ccctgatttc gcagttgtta
     9181 aatgttcata attatagttc catggttcat aataatcttt tattaatggc atatctgggt
     9241 tataaaaaat tttacctaaa gcaatttttg aaattctact tccagatttt aattcaagtt
     9301 tccctttacg atttagtacc caaccacctt tgtagtgttc ttggtcttcc caacgtttcg
     9361 gataccctac acctggcttc gtttctacgt tattgaacca catgtactca gcacctggac
     9421 gatttgtcca agtgttttta catgtcacac tacacgtatg gcatcctatg catttatcta
     9481 aatttaatac catcgcaact tgcgctttaa tcttcaagcc aattaacctc cttcatcttt
     9541 ctaactgcta catataaatc cctttggttc ccaattggtc cataataatt aaagtgataa
     9601 ctaatttgtg cgtatcctcc gactagttgt gttggtttca aatggattct agtcggcgcg
     9661 ttgtgtgaac caccacgtgt atctgtaatt tctgacccag gcgtttgaat atgtttatct
     9721 tgtgcatgat acataaacat tgtaccttta ggcatacgat gcgaaataac tgctcttgcc
     9781 gttacaacac cattacggtt atacacttct agccaatcat tatcttggat atcgtgtttt
     9841 tcagcatctt catttgatat ccaaaccgtt ggaccacctc taaatagtgt caacatatgc
     9901 ttattatctt gatacattga gtgtatattc cattttccat gaggcgttaa ataacgcagt
     9961 accaaagcat ctgtaccacc tttaattttc ttatctctat tcccaaatac cattggcggc
    10021 aatgtcggtt tatatactgg taagctctcc ccaaattgtt ggaaaacttc gtgatccaca
    10081 taataacttt gacgtcctgt taatgttcta aaaggtacta gacgttctat attcgttgta
    10141 aatggtgaat atcgtcgacc ttgtttattt gaacctggga atactgctgt cggtattact
    10201 tctcgtggtt gtgaagttat atttaaaaac gaaattttct cagcagcacg ttcgctagaa
    10261 atatctttta acggcattcc agtttgttct tcaagatctt catatgattt ttgtgataat
    10321 ttaccattcg tagcagatga aatacttagt attgcatctg ctacattacg tgctgtatca
    10381 atacgcggac gattcgctct cacagaatca tcatttgtat cactccacgt acctaacata
    10441 ctttttaatt cttcatattg ttcactgaca ccgaaactta caccatgtgc tccaactttc
    10501 cctttttcaa gtacaggacc aagcgtgaca tatttgtcgt aaattttagt gtagtcgcgt
    10561 tctacaattg caaagttagg cattgtacgt ccaggtaccg cttcaatttc acccttcgac
    10621 caatctttca ctacgccgta tggtgttgaa atttcttgct ttgtatcatg actaagtgga
    10681 gttgtcacaa catctttaaa cgttccaggt aaatagtctt ttgccatttc tgaaaatgct
    10741 tttgccaacg ttttataaat atcccagtct gaacgcgatt cccataatgg atcaatagct
    10801 ggattaaaag gatgtacata tggatgcata tctgtagatg acaaatcatg cttctcatac
    10861 caagtcgctg ctggcaaaac aatgtcagaa tataaaggtg ttgctgtcat tctgaaatct
    10921 aaagaaacga ctaaatctaa tttccctgtt gtttcctcac gccacgtaat ttcttctggc
    10981 ttttcatctt catttggtgt agctaataac cctgattttg tgccaagtaa atgcttcata
    11041 aagtattctt gaccttttgc agaacttgag attagatttg agcgccatat aaacagtgat
    11101 ttcggatgat tctttttcaa atccggatct tctatcgcaa attgcgtttg ttttgactta
    11161 acttcattta tcgctcgttt taaaattgcc tcattcgact caatgccttc atctttagct
    11221 tcttctgcaa acaacaaact atttttatta aattgtggat atgatggtaa ccaaccaagt
    11281 ctagctgcta aaacattata atcagctggg tgttgatgct ttaaatcctc tgttttagct
    11341 aatggagatt ttaaacgatc tacatttgac tcttcatatt tccattggtc tgttgcaaaa
    11401 tagaaccaac ttgttccatt ttgcaaacgt ggtggtcctt gccagtcttt cgcaaatgcg
    11461 acagtactcc atccttcaat cggacgacat ttttcttgtc ccacatagtg agcccaaccg
    11521 ccaccattca caccttgaca gccacataac ataactaagt ttaagattga acgataaatc
    11581 gtatctgagt taaaccaatg gttaataccc gcacccatga taatcattga acgcccttca
    11641 gtatcgatag cgttttgcgc aaattctttc gctacttgaa tgacaacact ttgttttacg
    11701 cctgaaatgg cttcttgcca agcaggtgta tattttgatt ctgcatcgtc gtatcctttt
    11761 gattctaatt tatgatcaaa acgacgcacg ccatattgac ttgccattaa gtcaaaaatt
    11821 gtagcaatac ggactttgtc accatttgct aaagtgactt gtcgagttgg aattggacga
    11881 ttgaatatcc catctccatc actatcaaag tatgggaatt gaattgtttc taattcgtat
    11941 ccaccttctg ccattgataa tgtagggtta attttagaac catcttctgt ttctagtttt
    12001 aagttccact tcttaccttc ttcccaacgt tgacccattg tgccattagg tactactaaa
    12061 ctatcgctga ttgcatcatg aataactggc ttccattcgc cttgctctgt tgtttgacct
    12121 aagtcactcg ctcttaaaaa tcgacccgct ttatatccat tttcatcttc atccagcatg
    12181 ataagaaacg gcatatctgt atattgttta gcgtaattta taaagcgttc attaggttga
    12241 ttaacataat gttcttgtaa aataacatgt gtcattgctt gagcaattgc agcatctgaa
    12301 ccaggattcg gtgctagcca gttatctgca aatttcacat tttctgcgta atctggtgct
    12361 actgaaatga cttttgtacc tttatagcgg acttctgtca taaaatgtgc atccggagta
    12421 cgtgttaaag gtacattaga gccccacata ataatgtatg atgcgttata ccagtcactt
    12481 gattcaggca catctgtttg ctctccccaa atttgtggag aggcaggtgg taaatctgca
    12541 taccagtcat aaaaactaag catttcacca ccaagcaaat tgatgaatcg agcacctgct
    12601 gcataactaa tcattgacat cgctggaata ggtgtaaatc ctgcgattcg atctggacca
    12661 tattttttta ttgtatacag taattgtgct gcgattatct ctgtaacgtc tttccaattt
    12721 gaacgcacgt gccctcccat acctcgggct tgcttatatt gtttggcttt gtcttcattt
    12781 tcaacaatag acgcccatgc agcaacgcga ttaccatagt tttcttctaa tgcttcagtc
    12841 cataaatccc agagttttcc acgaatatat ggatatttga ttcgaagcgg actgtattca
    12901 taccaagaga atgacgcacc tcgtggacat cctctcggtt catattcagg catatccgga
    12961 ccacaacttg gatagtcagt ttgttgattt tcccaggtaa tcacaccatt tttcacaaat
    13021 actttccaag aacatgagcc tgtacagtta acaccatgtg ttgttcttac ttctttatcg
    13081 tggctccaac gttctctgta cattttttcc cattctctac ttttactttc taggatcgac
    13141 caattcccat taaatttttc tgttggctta aagaaattca atccaaattt tcccatattt
    13201 atatcctcct acgtataaaa atacgatgtg tagatgtcgt gtttttaaat actttaaaat
    13261 gcccaagact attgctttaa ttagattgta cattttttca caaatataaa atattaggga
    13321 atcacctaat tacttaagga atttccctat caataacggg atttcattga aataatacac
    13381 aatcatgtat ggtcgtgctt attgccaatc taaatcgttc aaatttggca caacgacaaa
    13441 taaggcttca acacgaatat attctctcgg ttgaaacctt acttattcat ttatttttta
    13501 taaattagtg acataacact gtattagcat ctgcacgatc ggttgaaata tatgttacat
    13561 tttcttgctg cttaataaat gcatcatagt aatcatattg cgacgaatga tatgtgccat
    13621 tcgatgtatc atttgggttt agcaaacagc cataaccttc gtcatataaa tgttcacaga
    13681 gcataagggc gtcatgtcta gaaccactta ctacataaaa ttgctgagtc ggattagaag
    13741 ttgttgagac attttcagta taacccacta cttcgcctat aatacatata cctggtttcg
    13801 cctcaattgc gtaatgctcc agtttcccaa caatattact taaacgcccc ttaacaacaa
    13861 actcgttaaa acacgatgct tgaaagacaa tcgctatcgg ataatcaata tctgtgtact
    13921 gttgtatctg ggcgataatt ttccctaaac gtttaactcc catatatatc gccaacgtac
    13981 caccatttac taaagcgttg acatctactt cattttcttc tgaatcttta aagtgacctg
    14041 tagaaaatgt cacactctta gcaacagcac gcatcgttaa tcctgtttgc atcgtagcaa
    14101 ctgcagcact cgcagatgtc acaccaggta caatttcaaa cgcaatatta aagttgttta
    14161 gtgtgtcaac ttcttcttga actcgtccaa atattgctgg gtcgccacct ttaagcctca
    14221 caaccttgtt atatcgacgt gctgcttcca cgatacattc atttattttt tcttgctgaa
    14281 tgtgttttgc atacggcttt ttaccaacat cgataatttc agtagtcaaa ttcgcatatt
    14341 gtaaaattaa cggattcact aatcgatcat atagtataac atccgcttca cgtattaaac
    14401 gctcagcctt tttagtcaaa taattcggat tacctggtcc cgcacctatc aagtaaacct
    14461 tgccatattt atctacagac atatatatac gttcccgtct gtaacttcta cctcataaac
    14521 atctacacaa ccttcatcag gttcttgtac aatcccagta tttaaatcaa tcttttgatc
    14581 atgaagtgga cagaatacat attcaccact cactgttccc tctgacaatg gtccttgttt
    14641 atgcggacaa atattgtgaa tcgcgtgaat tgtaccactt tcagttaaaa acaatcctac
    14701 ctctttgcct ttgacaataa ccttttttcc aattaggggt gttaattcat ctatagttgt
    14761 cactttaatt ttttcttttg tttccatgta ttacaccttc tccacttcaa aaattttacg
    14821 tgcttgcgca ttgctagtta ttgcttccca aggttcagct tcgactgctt ttttagcatc
    14881 cataatgcgt tcaaatagtt cattttgtct ttctgggtca agtaagactt cttttacatt
    14941 ttcaaatcca agtcttctta accatggcgc tgttctttca gcatatatac ctgtttcgcg
    15001 ataatattgc atcaaagcac cacataattt gattacttca tcttctgttt ctacagttgt
    15061 taaaaattca gctttttcga cttctgtacc accattacca ccgatataga tttggaatcc
    15121 attttcaact gagataatac caaaatcttt aacacctgat tcaacacaac ttcttgggca
    15181 gcctgataca cccattttga atttatgagg tgtatcgatg tattcaaatg ttttttctaa
    15241 acgaatgcca agtcgtgtcg tgtattgcgt accaaatcga caaaactctt taccaacaca
    15301 gcttttaact gagcgtgttt tcttaccata agctgatgct gaacgcatac ctaggtcttc
    15361 ccatatattt ggtaattctt cttttttaac tccatacaaa ccaacacgtt gcgaacctgt
    15421 cactttaact agtggcacat gatatttctt agccacttct cctagacgaa tcagttggtc
    15481 tgcatctgta acacctccac gcatttgagg tataacagaa aatgtaccat cattttgaat
    15541 attcgcatgg taacgttcgt tagcaaatct tgattctctt tcatcttcat gatcatgtgg
    15601 ataaaccatg tttaaataat agttgattgc tggtcgacat tttggacatc cacctttatt
    15661 tttaaagttt aaaacatgtc gaacttcttt agatgttttt aaacctttcg ctcttatttg
    15721 cgttacaatt tgatcgcgtg tcaaatcagt acaaccacat ataccagcag gttttgcggc
    15781 aacaaagtca tctcctaagg tgtgctgcaa tatttgagca atttgcggtt tacatttacc
    15841 acatgaattc cccgcttttg ttttagccgt tacttcttca actgttgtaa agccattttc
    15901 cgtaatcgca tttactatag tacctttatc aacaccatta caaccacaaa ttgtttcatc
    15961 atcagccata tcagcaattg atagcgatgc ctcttctcca cctttagtaa gcaatgatac
    16021 aagtgtgtaa tcttcagtgg attcaccttt tttcatcatg ttataaaagc gtgaaccatc
    16081 atcgatatca ccatatagta ctgcaccaac tacattaccg tcttttaaaa agattttttt
    16141 atagttatta tcaacactat taaatatttc aataccttta atttctgcat tttctacaat
    16201 ttgaccagca ctatacaagt cacaaccaga aacttttaat gacgtaaatg ttgttgatcc
    16261 cttgtatccg ttcgtttctt tatttgttaa atgatcagct aatactttac cttgttcata
    16321 tagtggtgca acgagtccat aaactttgcc gttatgttct gcacattctc caactgcata
    16381 tacattgcta tcacttgttt gcatcacatc attgacaaca ataccacgat taacatctag
    16441 acctgattct ttggctactt ctgtgtatgg tcgtatacct actgccataa caactaagtc
    16501 tgccggaatc tcgcgtccat cagccaattt aacaccttca acatcatctt ctcctaagat
    16561 ttcagttgtg ttggcttgca tttcaaactt cataccttgc ttttctaaat ctgctttaag
    16621 catatttcct gctttacggt ctagctgcat ttccatcaac cattcagcta aatgtaacac
    16681 cgttacttcc ataccttgat ctaataaacc acgtgcacac tctaaaccta gtaagcctcc
    16741 accaattaca attgctttct ttttagtctt cgcaatgttc atcatttgtt cagtatcatc
    16801 aatcgttctc caaccaatta cactcggtaa tgtcgaacct ggaactggaa tgacgaatgc
    16861 tttagaacct gtcgcaaaaa tcaatttatc gtatgatact tcaataccat ttgcagtagt
    16921 aactgattga tttgctctat ctacttcaat tacaggatca ttggtaatta actcgatacc
    16981 atgttcctca taccactcat atggattcat aattgtttct tcaactgtca ttttattttg
    17041 taaaatattt gaaagcatga tgcggttata gtttggataa ggttctttac ctattaccgt
    17101 aatatcatat aaatcgttgg cgcgctctaa tatttcttcg attgttcgaa tgcccgccat
    17161 accgttacca atcattacta gtttttgctt tgccataaaa tatgcccctt tactccataa
    17221 tatttatttc aaaaaaaggt attaattttt cgttagtgct tttatatttt catcggaatc
    17281 attaagcttt ctaatctatc gttaatgatt tgctttaaaa ttgggtcgaa gttaattgaa
    17341 ggtgtgaagt gtatatctgt attaataacc atgtcattca tttgctgctt cactttgtta
    17401 acaagtcttc cgtcatataa aaataatggt acgacaatca atttttgata ccgtttcgag
    17461 atgctttcta aatcatgtgt aaaactaatc tctccatata gcgttctcgc ataagtaggt
    17521 ttattaatct gcaaatgttg agcgcatatt tgtaactctt cgtgtgcctt agtaaaattt
    17581 ccattaatat tgccgtgtgc aacaaccata actccaactt gttgttcgtc acctgctaat
    17641 gcgtcacaaa tacgttgttc aattaatcgt ctcattaaag gatgtgtgcc aagtggctcg
    17701 cttacttcta cctttatgtc tggataccgt cgtttcattt catgaacgat attcggtata
    17761 tccttgagat aatgcattgc actaaagatt agcaatggta caattttaaa atggtcaacc
    17821 ccactttgaa tcaacgtcgt cattaccgtc tctaaatcct gatgctcact ttctaaaaac
    17881 gcaatatcat agtgatgtat atcatctttt actaattcag aaataaatgc ttctaacgct
    17941 tgattctgtc gtccgtgcct catgccatgt gcaacaatga tattcccatt cacatttacc
    18001 aaccctttca cacgtattgt ataccaaatc attttgtttt tgtgaaaaga atcacattat
    18061 aatgtaaaat cagggaattc cctgatgcct gtagtcatgc atattcctta tacattttcc
    18121 ctttttgtta aatcaaaaaa agcgaccgat atatgaatcc ctactcaaca tttatttgag
    18181 caagcatcaa tatatcggtc gcttgtagtg tatattatta tcttaaaatg gtggttggcc
    18241 taatattgtt tcgtcaaagc gctcgggtat caatactttg cgcatgatca cacctaaatc
    18301 gccatcatca ttttcatgtt cgctgtatat ttcataacct cttttttcat aaattttaag
    18361 taaccacgga tgcaatcttg cagatgtacc taaagtaact gccgctgact ttaacgtatc
    18421 tcgcaaaaat gcttcttcaa cataagtaag taattggcta ccatagcctt tcccttcata
    18481 ctcaggattt gtcgcaaacc accagacaaa aggatagccc gaaatacttt tcacacttcc
    18541 ccaaggatat ctaaccgtaa tcgtagatat aatttcatca tcaattgtca tgacaaatgt
    18601 agtattttta tctatatttt ctttaacagc atctaaatta gcattaactg aaggccaatc
    18661 aatacctagt tctcttagag gcgtaaatgc ttcatgcatg agttgttgca atttttctgc
    18721 atcttgttca cttgcgagtc gaatcatcgt ttttgtcata ttaatcccca ctctttttta
    18781 aatgatttaa ccatatttta tttttaaaat aaatatccat caaagtgtat caataaattt
    18841 atcacatgtc agaaagtatg cttcatctga atacaccaat actctcatga aacttattaa
    18901 aaattactct ctcaacgtaa aaaaaccatt caaattcatg aatggtttgg aagaatgatt
    18961 cattgttacg ctatttaatc actacatctt aattattgtt gctctaaacg attacgctta
    19021 ccatttaaga aagcataaac gagacctaca aaaataccgc caccgacaaa gttacctaag
    19081 aaagcaaaaa cgatattttt taaaacatgt aaccatgaaa ctgcatcaag gttaaagaat
    19141 accatacctg catatagacc tgcattgaac acaacgtgct catatcccat gtatacaaag
    19201 accacgacac cacaagctat gaagaatgcc tttgttaagc cgcctttgaa ttgcatagag
    19261 atgaaaatac caatattaat aaagaagtta cagaaaatac cttttgtaaa aatattcaac
    19321 catgttgaat caacagtctt tttctgaact aaagctgtta aagcttgtgt catttcaggt
    19381 gtcatgacat gcgcaaattt cattaagaaa aataacacaa acccacctaa aatatttcct
    19441 aagaaacaat ataataaaat ccaagtcatc ttcttaacag aaacgacttt ataataccag
    19501 cctactgtaa agtacatgaa gttacttgtt aataattcag agttagttaa tacaactaaa
    19561 atcaaaccta aactaaacgc aatggctccc attaaattga taagtccatc tacgtgagta
    19621 ttcgcaaact gcgtctttat tccgaacata aatacagtaa cgattgagag taaaaatccg
    19681 gccatcattg ctttaagcat atatcggcca ggtgtttgct gtaccattac ttgcttcatt
    19741 tgtactgaag atgatattga atcaacaatt gcgcctgttg aatatgtatc ttcaactgtt
    19801 ttcttatttt ctatcacagt acttccccca tttactttga tcaaataaaa tttactttct
    19861 aattatatta gtaaatcaat gtaattgata gattaaattt cagaaaaatc tttttattct
    19921 ctcaaaaatg tagttttctt tcataaaaac agcgagataa atcgcgtgat tggctaatat
    19981 ctttatgatt taagatatta cgacgctcat tttatctcgc tttatgtaaa gtattactta
    20041 ttctttttta aatttgaaaa gtaatgtaaa ccatatgcaa atatactgta accaattgca
    20101 gtacgtaaaa taaaccttat aactttatta ggtacacatt ttgacgttac tgaaatagaa
    20161 gatagtagtg ttgtgaaaat atataatatt aaaactttga tataacgttc taccatcgaa
    20221 tcatccctca tttttataat attacaagat tattataaac cgaatgaaac aaaaaagttt
    20281 ttacatattt tcaacaactt tatttcaata aagcatcaag ttgatctaaa gtttgattca
    20341 taccttgttc aacacccatg tttatgactt gttgagcggc ttcttttgtt ggaaataccg
    20401 atgttgatgt cactgtcgtt ttcgttgtat tgctcttgga aaatgacaaa gtaattttca
    20461 tgcttggcat ttttgtatct tttgcacctt gaggtgtcgc aaatgagtca atatattcaa
    20521 ttaaatatgg acgcttaacc gttttatatt ctgctaatgt atagctggtc attgtaggtg
    20581 ttttaatcgc ataaaatgca tcaccgcctg aaacagcatt aaagcgaaat acttttgtgc
    20641 tagccccttt tggatgaaac catttttcaa ataattcttt tgtcgtatag gcatcaaaca
    20701 ccttttcaat cggtgcttct atcgttcttg aaaaaataat tttattatct tcaactttaa
    20761 tcgtcatctt cccactccct atcttttata tttactcatc ttaacatgca ttgagtaaca
    20821 tatgttacca aaccatttgt tataaactat agtaatatta atcattccct ctgttgaaat
    20881 tgataactac gaaataaaaa acacactcta ttcagctatt aacagttgag tagagtgtgc
    20941 aaacctgttg aattaatgtg ctaacatttc ttctttgatt tggtctttat ttaattttga
    21001 aggataatat gttggccagt tatccatttc ttttaataac gcgtcattat cattacccat
    21061 aaagatatgg aagtgttctg cctttttagg tgcaatgttg tgatcgctaa attggatgaa
    21121 tttcggtaag tctttatcat taccatcgac caatttaaac ataaatctca cgccacggtt
    21181 acctttagga tatttcaatg ttttcttacc attgtattca tatttaccag tgtgtttctt
    21241 accatcttta gtaaatgtaa tttcatttcc ttttatatca atgttagtaa tatcagtttt
    21301 atatcctttg tcataataag cttttaaatc tttagcagat ttcttcggat cattttcagc
    21361 tttatgttcc atcacttcat caagcgtacc gtcttttaag taagggtaaa cagattgcca
    21421 ttcaccagca taatcgctta attcacggtc tttaacttgc tcatctttaa aatacccatc
    21481 agaaattgct ttatcgtgtt tactttcagc tttgtcgtct ttcactttaa caccactgtc
    21541 taaagcttta ccgatatttt caatattcga tttcattaat gattgatacg taatattatc
    21601 ttttttctgt tgttctttat ttaaagactc catgttgtag aattttaaag gcttcgcatc
    21661 tgtttcttta cgaattgttt cagtcacttt attcgcaaca ttatcttcgt aaagaatata
    21721 tttagcattg ctatctctaa tttctttaac aattttagtt aattcttttt gtgatggatc
    21781 ttcagcattc atattttgaa tacctttttg aacaaaacca taacgatcag ctaagtatcc
    21841 aattgattca tgtgaaataa atactgcatt accttgctta tcttttgtaa cttgcttcat
    21901 atcgttatca attttcttaa gatcgtcgtt taattttttg tagtttttct catagtcatc
    21961 tttatgtttt ggatcttttt tcactaattc atctttaatt tctttagcga aagtttggtt
    22021 aattttagga tctaaccata cgtgtggatc atatccacca tgatgatgat gttcttcttt
    22081 ctcatgatca tgcccctcat gttcatgctc ttcaccatgc tcgtgttgat cagttaaaag
    22141 ctttgcttta tctaatttat cctctaaaga ccgtttttta tctttatctt tgatagtaga
    22201 tgcaactttc ttagcaaccg gatctaaatt atcccctgta tacataaaca agtctgactt
    22261 gcttgcactt aatatatctt tttgtgttgg ttcatagcta tgtaaatctg tccctgctgg
    22321 atagattgat gataccttca cgtgttttcc accaatttgc tctgcaaatg attgcaatgg
    22381 ataaactgtc gttttaattg ttaccttgcc atctttatct tttccatcat cattcccaca
    22441 tgcggctaat gataaagtta cggctggtac aagaagtaac atacctaatt tctttttcat
    22501 agtcaccctc ctaaatagta atctttacgt tttaaaatta tatactcatg ctacttaaaa
    22561 tacaagcact gtttttataa ataatataaa gtcgaatcat actttaggta tttaccactt
    22621 ttcgttttat ctaatacatg actattgtac aaatatgata cagaaaactt tatagtattt
    22681 acgaacgtca taatataaga aaaaaagaca atctctatca tattgctaat agaaattgcc
    22741 ttttcatatt gtcctaaaat aacttaccct atttttatag tgtgactttt atatttaata
    22801 atggtattta catgatgcta tttttatatg attgtcatct actgtataaa ccaatctatg
    22861 ttcgtgattg atacgtctac tataataccc agtcagattc gattttaact tttcaggctt
    22921 acctatacct tccaatgcac catttctgtc aatactttta agtaactcat taatcttctt
    22981 caccattttt ttattgttct gctgaaaata cttataatct tcaaaggctt gaggcgaaaa
    23041 cgtaatattt aacctagcca ttatacatct atatctttcg ttatagtttt cccacgttct
    23101 agatctgcaa ttgattgagc taagtgttca gcattatttg ggttctgttg gaggtaaagt
    23161 gtttccatca tggagttata atctgattct gacatgatta ctacgttttt atcatcagta
    23221 gatgttacag ttaccatatc actatcatca tttacttttg tcataagtgc ctttaaattc
    23281 tgtcgagcgt atgaataatt tttaataatc atatttatcg cctcctttta acacacctca
    23341 attgtacaaa tatctgtaca ataaaacaag cgacacagaa aaaaactact aatcaagatt
    23401 tcgcacgggg aagaatcaat acctatcctg tactgattcc ctactaaaca atgtttacat
    23461 gataatttct acctatttag acttcataca ttaaaaagac aatctctatc atatcgctaa
    23521 tagaaattgc cttttcatat tgtcctaata aattgatatc acttacttat ctcaacaatt
    23581 aagactattt tattttatct tttaataact tctcataact ttcataatca tatggatctt
    23641 caactttctc gccattaata aatgcagttg gtgtcgtttt tatatgatta tctttcgcta
    23701 tttttttatc tttctctgca gctttccaag acttgctatc ttttgtcttg taatctttta
    23761 taattttatt ttccgtctct ttatctaaat gcagttgttt aatatgttta tctaatagtt
    23821 cttttgttaa ccattcttta ttttcatctt gctgggcagc aaataattgc ttttgaaaat
    23881 ctaaaaatga tttaggtgca tacatcaata ctgcatgact cgcacgcgaa ccaacaattg
    23941 agtctttacc taagaaagct aaattgacaa attggtattc cactttgtga ttatctatat
    24001 aatttttacg caactttggc atgacttttt catctaattc tttacaataa ggacatttat
    24061 agtcgccata tatgacaact aatggtttgc cgtttttcga ggatgtcgtt gctgattctt
    24121 ttttaccgca agctgttaaa attaacatgc tcactataaa taatgttagt aattttttag
    24181 tcataataac tcctgttcat atctagttcg tcatattttc ttcatgataa tccgcatcat
    24241 gttgttgata atatcgtctc gaatcaaaat cttgcttata tgatacacgt ttatcactaa
    24301 aatcatatgc ataataatgc acttcatcat cattactcaa aggtttataa gctaaaatga
    24361 taattttatc cttatcatag acataaatat tagcatcttt gcgttcaaaa tgatcaacac
    24421 gatcaccgtt gccttttttg gcaagttgtt cttgtttttg attttgaatt ttaactgctt
    24481 catctatttc tttcacatat ttatcattac cacatcctga tataaagact agtaataata
    24541 ataccgtcgc cacaaatcgc ttcaatgctc ccactcctac tatcaacttc caactaactt
    24601 tcaatataat taatactata caccgtcaaa cattaatgcg ctacaactac taccaatgca
    24661 tttaatataa atatccaaag aaataacagt tatgtatagt caaagaaaaa tatatttcat
    24721 tttctatgtg tatttgtaat cagtttttga taaaataaaa aaatagcatt attaataaat
    24781 tcaaaaatta cagttcaatt cagagaggga tgggataaaa tgaactttgt aacgttgact
    24841 tcggatgagt tcaatgcgtt tacaacaaag catttttcac attacacaca atcagctatt
    24901 cattacaatc atagagttga tttaaaaggc gatgtgcatc ttgtaggggt taaagatgac
    24961 aatggtcaag tgattgcagg atgcttattg acagaagcac gcacacttaa atttttcaaa
    25021 tatttttata cacatcgcgg gccagtgatg gattatacaa atcaatcatt agtagcattt
    25081 ttctttaaag cattaacgtc atatttaaag aaacacaatt gtttatatgt ccttgtagat
    25141 ccatatttaa ttgaaaattt acgcaatgca gacggtgaaa ttgttaaatc ttatgataac
    25201 cgagcatttg ttagaacaat ggataaatta ggttataaac accaaggttt ccctgtaggt
    25261 tatgattcaa tgagccaaat ccgttggctg tcagtgttag atttaaaaga taagactgaa
    25321 gaccaacttt taaaagaaat ggattatcaa acgagacgta atattaaaaa aacatatgat
    25381 attggtgtca aaactaaaac gttaacgatt gatgaaacgc aaactttttt cgacttattc
    25441 catatggctg aggaaaagca cggtttcaaa ttccgtgagt taccatactt tgaagaaatg
    25501 caaaagttat acgatgacca cgccatgtta aagttggcgt atattgattt aaacgagtat
    25561 ttaaaaacgt tacaattaaa gcaacaacaa ttaacagctg aactctcagg tgttgaagaa
    25621 gcattggaag aaagtcctaa ttcaaagaaa aataaaacga aacgcacaca gctagaacaa
    25681 cagctaaata gcaataagcg taaaatcgac aatacaatag aacaaattga acaagatggt
    25741 gcagtcttaa acttagcttc tgctttattt atctacaatg agcatgaagt ttattactta
    25801 tctagtggtt caaaccctaa atataatgct tatatgggtg cctaccattt gcagtgggaa
    25861 atgattaagt ttgctaaagc gcatcatatt gatcgctata atttttatgg tattaccggt
    25921 gactattcag agtcatcaga agactatggc gtccaacaat tcaaaaaagg ttttaacgca
    25981 catgtagaag agtatatcgg tgactttatt aagcctatca aaccattact atataaggta
    26041 caaacgtact taaatcataa acgtcgttaa aatatcgctc caagattatg acaacataac
    26101 aaagaaccac gcatcatgta tatggcatca tcaccatact aaaacatgat acgtggtttt
    26161 atttgattat tcttcattta taacatttaa gaaacgtctt aattcttctg ttttcggacg
    26221 attgaacatc tcttctggag ccccttgttc tccgatcatg ccttcatgaa taaatacaat
    26281 gttattagat acttcttttg caaaacgcat ttcatgtgtc acaatgacca ttgtcatgcc
    26341 ttcattagcc aaatctttaa taacctttaa aacatcattc acaagttcag gatcaagtgc
    26401 agatgttggt tcatcaaaca acatcacttt agggttcatt gctagtgctc ttgcaatagc
    26461 aacacgttgt tgttgaccac ctgataatgc atgtggacgt tgatctttga catgtgttaa
    26521 accaactttc tcaagtaact caagtgattt cccacgtgcc tcatcctttt tcaacttttt
    26581 aactgtgata agaccttcca ttacattttc taatgccgtc ttatgcggaa aaaggttata
    26641 actttgaaat accatgccag actgtttacg aacttctatt tgtgattttt tatctttaga
    26701 tgtatatgtt ttgccgttaa cataaactgt accttcagtt ggaatttcta atgcattaat
    26761 catacgtaac aatgttgttt tacctgaacc agatcgaccg attaaggtta caacctcacc
    26821 ttgttcaaca gataaatcaa tacctttgat gacttcaaca tcattaaatg atttatggat
    26881 attgttcaat tgaatcatga gcgataccct ctttcaatat aagattcata gataccttgg
    26941 ataattgaaa taataaagca taccacccag tacattagcg caactaacac ataaattgtt
    27001 aaatattcat atgttgtaga cgcaacttct tgagcttttc taaacatttc agccactaaa
    27061 ataaatccta ataatgatgt atctttgatt aaacttaaaa atgtattacc tagtgcagga
    27121 atcgacactc gaattgcctg cggtaaaatg atacgttgta tcgtttgacc atacgtcata
    27181 ccgatggaat atgcagcttc tgtttgtcct ttcggtatag aaataatacc gccacgaata
    27241 atttccgaag catacgcacc tacatttaat gataaaccaa taatagcagc cactacaggt
    27301 gccaatgtcc attggttgtc agcgtcattt gttaataatc tacctaattc tggaatacca
    27361 taaaatatga taaatagttg tactatcatt ggtgttcctc gaataataga tacatagaca
    27421 cgtgaaatac ttcttaaaat tttactcgtt gaaattcgca ttaatgctgt aaataatgca
    27481 ataatcaatc ctaaaacaaa tgtaactaat gtaataggaa ttgaatattt gaccaaacct
    27541 tctagcatag gtccgaaagc ttgttttgca gcatctagcg catgttgttg ttcgctattt
    27601 agatttagaa acatcttgac caaaccattt cttacctatt ttagctaatt caccgttttc
    27661 ctcgattttt ttcaagccat cattgaattt ttgaactgtt tcatcatctg cttttttaga
    27721 aaatgcaaat gcagatctac tttgttcagc attacctttg attgctttga tcttagcatt
    27781 aggtttttgt tttttataat ccaagtatga cagactatca ttaaatgtac catcaacacg
    27841 cttagacaat aataaatcca ttgattggtt aaagccatca acttttgtaa tatcagcacc
    27901 tttatcctta gctaatttac cataattaga tgtgaatgtt tgtgctaact tcttaccttt
    27961 aacatcatca aaatctttaa tatctttttc attttcacga ataactaaaa cagcacttga
    28021 gaatgtgtaa ggcttagaaa atttatattt cttttctcta tcaggattaa tacctacttg
    28081 gttcgcgatt acatcaaaac gccctgcgtc taaacctgca aacatagaat cccaagaagt
    28141 ttcattaaat ttaagtttta aaccttcttc tttagccact gctttaataa catcaatatc
    28201 gtaaccagtt aatttaccat ctttatcatg gaaactaaat ggtgcataag tcccctcagt
    28261 accaacatta attgtcttgc tatctttatt agcttccttg tctttcgacg aattgtttcc
    28321 acatgcagcc aatatgaaaa cgaaagctat catcacaaat aaaagtcttt tcatagaata
    28381 tatctcctta ttcttattat tctaatcgga ataaaaatat tttaatctga aatgccggat
    28441 aagtcaatat gttgaacttt tgctaaaaag ctttatatag ctagcaactt atatttattg
    28501 acgagaatca acttcttttt tcttcccttg taacataaat gaacaaaaga aactaacaac
    28561 tgtaatcaat aatgcaaata aaaatccagc gtgataacca tgcaacatcg tatcaacttt
    28621 aatttgattc atcatatcaa ttttgttcat gccattatag tctgacatag ttggagaaaa
    28681 gtttttgctt gcatgagata aaatagtaat taatgcagct gttccgatag aaccagcaat
    28741 ttgttgtact gtgtttgtca tagatgaacc gtgtgcattc atttctggct ttaattgatt
    28801 catcgtatgg gtcattactg gaattaatcc taacgcgata ccaaccattc gaatcgcata
    28861 aactgttgcc aacatgattg ttgatgtttg ttcatccatt acaacaaaat aagccgtagt
    28921 aataacaaca atcgccatac ctactaaagc aagctttcta ggaccaaact tttcatataa
    28981 agcacccgaa gtcatcgaca taaatgccat aacaattgct cctggtaata aagttaatcc
    29041 agaatctaaa gctgaacgat gcaataaatt ttgcacaaag ataggtaaaa tcgtttccga
    29101 accaatcatc gataccattg tgacacccat aataatgata ccaactgtaa attgtttatt
    29161 tttaaataca gcaaaattta ataatggtgt tgatagccga tattgacgta atacaaataa
    29221 tgcgactaaa acaacgcctc caatcataga tactaatacg ataggattat caaaaccttt
    29281 ttctgaaatt gaactcgttc catacaataa cccgccgaaa cctagtacag aaaaaatgac
    29341 agaaataaaa tcaataggta ctttaattgt agttccaaca ttttttatcg ttttaaatcc
    29401 aattaaaaat gtaacagctg caattggagc gacaacatgg aaaagcgatc tccagtttaa
    29461 atattcaaca aggtaacctg aaagagtagg tccaatagct ggtgctaaac caattaccaa
    29521 accaaatatc cccatatatt taccgcgttc atgcggttgg aaaacatcca aaatcgttgt
    29581 catcatcaat ggcatcataa tacctgcgcc taacgcctga ataattctag caactaataa
    29641 tgttgcaaag tgcggactca agccgccgag taatgtacct aagaaaaaga tagaaatacc
    29701 cactaaaaac acttgtcttg ttgtataacg ttgtataaca agcgcggaca aaggaataac
    29761 aatcccatta gtcaataaaa aagctgttgt cagccattga acttgtgtat atgaaatttc
    29821 aaaatctttc ataatacttg gtaaagccgt cactaatagt gtttcattta ataatccaaa
    29881 aaatccaccc atcagcatag taataatcat cgttaatttt tgtttcttcg acatttaaag
    29941 cttccccctc accatctaaa aaacgacaca catttttaga tatccggtaa ttatacccat
    30001 tttccaaaaa attagcacga ctttattaca atttctttaa aacgtaatca ttactattta
    30061 catatgagta gttatcggtc ataatatatt tacaaggagg gatagagatg aaattagatt
    30121 tacagactgc tcgtcgaaat ttaaatagcc ctaacattaa aacaagaaag cgtgctttaa
    30181 agattattaa gcaacacaaa agagcgaaat aattgtttta atttgactat attgtatgtc
    30241 taactatatt ttcgagtatc cctagctcgt taatataaac tctatactta actatttcct
    30301 tacattactt cacatattta agagctgcat atacactcac tccttgtgta catgcagctc
    30361 tttttttata agtagtattt atctataact tctaaatcat ccgttaattc ataaacaagc
    30421 ggtgcacctg ttttaatttc ataattaatg atatcttcat ctgacacatc ttcaagatat
    30481 ttaattaatg cgcgaattga atttccgtgt gcagaaacta ataccgtttg accatctagc
    30541 aaatattgtg aaatatgatc tgtccaaaat ggtatcactc gaactaaagt atctttcaga
    30601 ctttcagaat aaggcatcat acgtttatct aaatgattat atcgacgatc agctaagtaa
    30661 gcttcacgtt gttcttcggt ttcagcaggt ggtttcacat cataagaacg acgccaaata
    30721 tgtacttgtt cttctccaaa ttcttttcta gcatcatctt tatttaagcc ttgcaatcca
    30781 ccatagtggc gttcatttaa acgccagctt ttatatacag gaatccattg ttgtttagat
    30841 tcagttaaaa tataatgcgt tgtatctaaa gcacgtgtta ataacgatgt aaaagctaca
    30901 tcgatggcaa tgttattttc tcttactttt tcacctgctc tagtcgcttc attaatacct
    30961 tgttcagata aattaacatc ttcccatcca gtaaataagt ttttagcatt ccactcgctt
    31021 tgtccatgac gacataaaat taattttggc ataacttata acctctttcc tttaatcttt
    31081 aggacgttat ttcagggata ttttaagatg atttaatctt agtaattttc ttttcaatat
    31141 at
//
LOCUS       DAHHSC010000012        29938 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.012, whole genome shotgun sequence.
ACCESSION   DAHHSC010000012 DAHHSC010000000
VERSION     DAHHSC010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 29938)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29938)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29938
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.012"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            122..1522
                     /locus_tag="NBI57_000425"
     CDS             122..1522
                     /locus_tag="NBI57_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCD1821408.1"
                     /translation="MDTSKQFRGDNRLLLGIVLGVITFWLFAQSLVNLVVPLQSTYSS
                     DVGTINIAVSLSALFAGLFIVGAGDVADKFGRVKITYVGLILNVVGSLLIIITPLPAF
                     LIIGRIIQGLSAACIMPSTLAIINEYYIGTRRQRALSYWSIGSWGGSGICTLFGGLMA
                     TYIGWRSIFVVSILLTLLAMYLIKHAPETKAEPIKGMKAEAKKFDVIGLVILVVTMLS
                     LNVIITQTSHFGLVSPLILGLIVVFICSLVGFVYYENKIKHPLVDFSIFKNRGYSGAT
                     ISNFLLNGVAGGALIVINTYYQQQLGFNSSQTGYISLTYLIAVLSMIRVGEKILSQHG
                     PKRPLLLGSGFTVIGLILLSLTFLPEVWYIISSIVGYLLFGTGLGLYATPSTDTAVAS
                     APDDKSGVASGVYKMASSLGNAFGVAVSGTVYTVLAANLNLNLGGFTGMMFNALLAIV
                     AFLVILLLVPKNQTNL"
     gene            1748..2683
                     /locus_tag="NBI57_000426"
     CDS             1748..2683
                     /locus_tag="NBI57_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HCD1821409.1"
                     /translation="MKIAIAGSGALGSGFGAKLFQAGYDVTLIDGYTSHVEAVKQHGL
                     NITINGEAFELNIPMYHFNDQRDESIYDVVFLFPKSMQLKEVMEDMKPHIDNETIVVC
                     TMNGLKHEEVIAQYVAQSQIVRGVTTWTAGLESPGHSHLLGSGPVEIGELVDEGKENV
                     IKVADLLNEAELNGVISKDLYQSIWKKICVNGTANALSTVLECNMASLNESSYAKCLI
                     YKLTQEIVHVATIDNVHLNVDEVFEYLVDLNEKVGAHYPSMYQDLIVNNRKTEIDYIN
                     GAVATLGKQRHIEAPVNRFITDLIHTKESQRHAQD"
     gene            3005..3856
                     /locus_tag="NBI57_000427"
     CDS             3005..3856
                     /locus_tag="NBI57_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HCD1821410.1"
                     /translation="MSKIFVTGATGLIGIKLVQRLKEEGHEVAGFTTSENGQQKLAAI
                     NVKAYIGDILKADTIDQALADFKPEIIINQITDLKNVDMAANTKVRIEGSKNLIDAAK
                     KHDVKKVIAQSIAFMYEPGEGLANEETSLDFNSTGDRKVTVDGVVGLEEETARMDEYV
                     VLRFGWLYGPGTWYGKDGMIYNQFMDGQVTLSDGVTSFVHLDDAVETSIQAIHFENGI
                     YNVADDAPVKGSEFAEWYKEQLGVEPNIDIQPAQPFERGVSNEKFKAQGGTLIYKTWK
                     DGMNPIK"
     gene            complement(3948..5444)
                     /locus_tag="NBI57_000428"
     CDS             complement(3948..5444)
                     /locus_tag="NBI57_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HCD1821411.1"
                     /translation="MTTKKLYFLSISIIILVAISIAIYITLNSNTKTRLTNDSQQQID
                     KIIEHDLQKGHIPGASILIVKNGKVFLNKGYGYQDVDKKVKASPTTKYEIASNTKAFT
                     GLAILKLAQEGRLNLNDDVSKHVPHFKMNYNGQNETITIKQLLAQTSGIPSDITSEDA
                     VTNKNNRLNDVTRAIMGDELHHKPGEEFEYSNMNYDLLGLIIQNVTKQSYTKYITNSW
                     LKPLHMTHTSFKQTNNKSKHDAIGYELQGSTPVVSKPEFNLWDTPSAYMMTSTEDLEH
                     WIKFQLNPPDKYKSLVQQSHKNLSSTIGEPNANAYASGWFTNNDEHLVFHSGTLDNFS
                     SFILLNPKQNYGIVVLANLNSEYVPKLVEHLNTQIVNHKRYSTVASILNQYKDQFNIV
                     TVLMTTLILLAFIFSAYRAWQMRHGQILLRRSKRIAVLSWLTLCLCIAIALILYALPY
                     LILGSNNWSFVLTWLPIEIKLALITTLIALFSTLIVILLFLHTKITKT"
     gene            5676..7505
                     /locus_tag="NBI57_000429"
     CDS             5676..7505
                     /locus_tag="NBI57_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HCD1821412.1"
                     /translation="MFNQFKRLIIGQPKKNRELKDEKISKFKGLAILSSDALSSVAYG
                     PEQILITLSVVGAVATWYTLPIAGAVLILLAALIMSYRQIIYAYPKGGGAYMVSKTNL
                     GEKWGLLAGGSLLVDYILTVAVSISSGADAFVAAFPSLYGHKVLIACLLVLFILILNL
                     RGLTESATVLSYPVYLFIIGLVILIFIGTFRVATGDIQPHMHASVGTAVPGVTLFLLL
                     KAFSSGASSLTGVEAISNAVTNFREPSANNAVKTLIAMGSILAFLLVGIVGLAYVYGI
                     MPQTETTVLSQLAMQIFGDNAAFYFVQATTVMILVLAANTGFTAFPMLAASMSKDKYM
                     PRMFTVRGDRLGYSNSIIILGVLAIILIIVFDGMTEDLIPLYAVGVFIPFTLAQFGMV
                     IKWIHERPKNWLSKLSVNLLGGIVTFIVFMILLITKFSQVWPILIFLPFVVIFFLKIN
                     KHYRDIAEQLRSDIDVLNVDVVDRNLAIVPITSITTAVDKSIYYAQMLANNDVIAVHV
                     SFGDEDEKAFQEKWKRHFPDVRLVILHSEYRSIIRPISRFIDKINRKANDQNYMITVV
                     IPEFITKKRWHNLLHNQTSLRMKLYLIYQKNVNVCTIPFKLKK"
     gene            complement(7646..9724)
                     /locus_tag="NBI57_000430"
     CDS             complement(7646..9724)
                     /locus_tag="NBI57_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HCD1821413.1"
                     /translation="MALLEAFLIFIFAVIISSVINNRFPQIPTAFIQIALGVVIFIIP
                     IQVDFQFNSEVFMFAVIAPLLFVEGTHVSRTKLLEYRKPILLMSMALVFATVVGVGYF
                     IHWIWPALPMPAAFAIAAILCPTDAVAVSAITRGKLLPKGSMTILEGESLLNDAAGII
                     SFKIAVTALVTGTFSLFQAVEQFIISTILGVLIGAIIGFVVVRIRIDLTANKGLKDNN
                     TLTFIQLLTPFVVYFLAEEVHASGIIAVVIAGLIHGLERDRLIRAQTELQMNYHQIWN
                     TFSYALNGFVFVVLGFMIPTVVIDIFQTEPDNFSFLIVITILIAIAIYACRFVWVYFW
                     YKDFYFPKNIQSYLDEEHDSHETPPSRVRYAFIMTMCGIHGTISLSMALTLPFIITKG
                     QAFEYRNDLLFIASFMVLISLILAQIVLPLITPSAEDTTFKGMTYQSAKIFIVQKVIQ
                     HFKNESKKDKNDTNYRPVLNQYYGELLFLLNSEPNNQNTKELKRLEDIAKVIETSTLE
                     RLIDKGKATYQDINNYRNIVELTETHRTASFVEKFANFFKMLWLRLKAMKHYKALNKE
                     SKKQEFENSFKDVQKIMRIVNHNIILRLKEEQNSTNVLEVSLVINHYYDMSRSLKWRA
                     QRRKERQENSNQIIPQAMFHNHKLEALYLQRHLLDELIRKNKINNIVAAQIRENINYN
                     EIVLSLQSKH"
     gene            complement(9862..11271)
                     /locus_tag="NBI57_000431"
     CDS             complement(9862..11271)
                     /locus_tag="NBI57_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCD1821414.1"
                     /translation="MARKLHRELNNRHIQLIAIGGAIGTGLFLGSGQTISLTGPSLLF
                     TYMIIGVVLFAFMRALGELLLSNTRFNSFVDIANEYLGPFGGFVIGWTYWLCWIVSSM
                     SDLTAMGQYFAFWYPQVPNWITVLFIVLILISFNLLGARLFGELEFWFSIIKVVTIIA
                     MVIVGLVLIFFSFKTHYGHASFTNLISHGGMFPGGTFGFLMSFQIAVYSFIGIELIGV
                     TAGETKDPEKTLPKAINNVPIRILLFYIGGLLVIMSVIPWNDIDPNSSPFVKLFTLIG
                     VPFAAGVVNFVVLTAAASATNSGIYSNSRILFGLSQQGLGPKVLNKTNSHGVPYLSML
                     VSSIALLIAALLNYIFPNAIQLFIYVTTLSTVLFLVVWAMIIVAYLMYLKKHPEAHKN
                     SKFKLIGGKPIAYIILAFFFFVFILLFFSDETRAAIYISPFWFIFLFFFYKKYKTNAE
                     KLAYEQRQNDSGHFRYDNQ"
     gene            11557..12363
                     /locus_tag="NBI57_000432"
     CDS             11557..12363
                     /locus_tag="NBI57_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HCD1821415.1"
                     /translation="MKIGTIADLHIDRHNKKTSEDYLEALVEIVKYKKLDILLIAGDI
                     SNHYQLTHQFITQLTKQLDIPVKFVPGNHDLWEVESMTTQDIWNNYKSMSQCLVGKPF
                     IVNEEWAIIGHTGWYDYSFAAQRFSLEHLQKGKHYGATWQDKERISWGISDQNLSKIA
                     AEQVKKDILEVGNRRVILVTHVVTHPDFIVPMPHRIFDFYNAFIGTSDFNPLYAMFDI
                     PYSIMGHVHFRKSVMDDGRCYLCPCLGYPRQWRSEDIYQEINETIQIIEI"
     gene            complement(12426..13082)
                     /locus_tag="NBI57_000433"
     CDS             complement(12426..13082)
                     /locus_tag="NBI57_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001080277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HCD1821416.1"
                     /translation="MNRKILPVKPISQLFPIPMVMGCEGVSAAMMLQYNNQHIPATEI
                     MRHWPTHPNNPHKGYVGHHLFIKLGNYHQTIFPEAYVPFLQKYNPNIVDGTGKSLEDL
                     KGIIDQGQPVLIYHTNLGSKPLLRVFRFDNKPAKQVSNIHVTLLIGYDDYYYYYIDPL
                     WSHIRRGLVLPAIIPNRKQIIKIRKEKMEYSFNSPGRKCIYVQPHSYTIENQQQNKHT
                     "
     gene            13195..13668
                     /locus_tag="NBI57_000434"
     CDS             13195..13668
                     /locus_tag="NBI57_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HCD1821417.1"
                     /translation="MEYQLQQLASLTLVGIKETYENGRQAQQHIAGFWQRCYQEGVIA
                     DLQLKNNGDLAGILGLCIPELDGKMSYMIAVTGDNSADIAKYDVITLASSKYMVFEAQ
                     GAVPKAVQQKMEEVHHYIHQYQANTVKSAPFFELYQDGDTTSEKYITEIWMPVKG"
     gene            complement(13791..15002)
                     /locus_tag="NBI57_000435"
     CDS             complement(13791..15002)
                     /locus_tag="NBI57_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug effflux MFS transporter"
                     /protein_id="HCD1821418.1"
                     /translation="MQSTKTKTKHFSFLLLITLGVMTAFGPLTIDMYVPSLPKVQGDF
                     GSTTSEIQLTLSFTMIGLALGQFIFGPLSDAFGRKRIAVSILIIFILVSGLSMFVDQL
                     PLFLTLRFIQGLTGGGVIVIAKASAGDKFSGNALAKFLASLMVVNGIITILAPLAGGL
                     ALSVATWRSIFTILTIVALIILIGVASQLPKTSKDELKQVNFSSVIKDFGSLLKKPAF
                     IIPMLLQGLTYVMLFSYSSASPFITQKLYNMTPQQFSIMFAVNGVGLIIVSQVVALLV
                     EKLHRHILLIILTIIQVVGVALIILTLTFHLPLWVLLIAFFLNVCPVTSIGPLGFTMA
                     MEERTGGSGNASSLLGLFQFILGGAVAPLVGLKGEFNTSPYMIIIFITAILLVSLQII
                     YFKMIKKQHVA"
     gene            complement(15184..15843)
                     /locus_tag="NBI57_000436"
     CDS             complement(15184..15843)
                     /locus_tag="NBI57_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1821419.1"
                     /translation="MKRTDKYRDSYQYDNQNQNHRRQSEDASYRQQYAKGDPEEHPER
                     YYNGRDYRREQILEEENEKSRRSKKWLYIIIAILLIIVAIFVTRALLNNDSDKVSNDP
                     KVSQNYKKQVENQDGQINQQVDNAKENIKNNQKTDDIIKNLQNQIDNLKQQEQNKADS
                     KLTQFYQDQINKLTEANNALKNNASQGKIESMLNDINTKFDSIKSKLESLFKDDNGGA
                     N"
     gene            complement(15903..17045)
                     /locus_tag="NBI57_000437"
     CDS             complement(15903..17045)
                     /locus_tag="NBI57_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HCD1821420.1"
                     /translation="MQLQKIVIAPDSFKESMTAQQVGNIIKQAFTNVYGNTLHYDIIP
                     MADGGEGTTDALMHATGATKYTVIVNDPLMRPIEACYARADEQQIAIIEMAAASGLDL
                     LEKEERNPLYTSSYGTGELIKDALNHGAKTIILGIGGSATNDGGTGMLSALGVKFTDV
                     NGDLLQMNGANLAHIAQIDITNLDSRLKEVTFKVACDVSNPLLGENGATYIYGPQKGA
                     DAKMIPKLDFAMSHYHDKIKMCTGKSVNQIPGSGAAGGMGAALLAFCETTLTKGIDVV
                     FDITDFHQRIKDADLVITGEGRMDYQTIFGKTPVGVALAAKQYHIPVIAICGSLGENY
                     QHVYDFGIDSAYSIISSPSTLEDVLQNSEQNLLNTATDIARILKLQ"
     gene            17313..17699
                     /gene="gtxA"
                     /locus_tag="NBI57_000438"
     CDS             17313..17699
                     /gene="gtxA"
                     /locus_tag="NBI57_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase GtxA"
                     /protein_id="HCD1821421.1"
                     /translation="MKLTQTHAEILKFIIVGGINTLNYYVVYLLLLKLLHIEYMISHI
                     TGFLVAFVISYYLNCYFVYRVKPTWRKFISFPITQIVNVSLQTVLLYVFVSWLNLPAE
                     IAPFAGLVITIPITFVLSKWILKDSN"
     gene            17833..17940
                     /locus_tag="NBI57_000439"
     CDS             17833..17940
                     /locus_tag="NBI57_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HCD1821422.1"
                     /translation="MFNLLINIMTSALSGCLVAFFAHWLRTRNNKKGDK"
     gene            18643..20406
                     /locus_tag="NBI57_000440"
     CDS             18643..20406
                     /locus_tag="NBI57_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCD1821423.1"
                     /translation="MNNQTNWIKILSGFASDSKWKIMLSILLSIISVFSGLVPYWAVF
                     KIILMMINNAYTINSIMVYIFIALITYISQVCCFGASTMLSHITAYEILSEIRKKLAQ
                     KLMRLPLGVVESKKIGELKNIFVDKVETIELPLAHMIPEVIGNLLLSAAIFLYIMLID
                     WRMASALLVTIPISIFAFKKVMSGFNETYAEQMKSNNYMNSAIVEFIEGIEVIKTFNQ
                     SQSSYKKYKDAVDNYKIHTLNWFKNTWGYMNLGASVLPSTFLGILPVGMYLISINQLN
                     YAEFFLCIVLSLGVVAPIKNFTNYVNHLKSIQYALTEVNQILSLEELVLSTKFKKPQH
                     YEIAFNNVGFSYNKDKDDLVFKHLSFTVPENNFTAIVGASGSGKSTIAKLISRYWDVT
                     SGEITIGGINIKDIESKQLNDLVGFVGQDNFLLNLTFKENIKLGNPEATDEAVEKAAK
                     LAQCHEFIEKLPDGYDTNVGTVGDKLSGGEKQRVTIARMILKDAPIIVLDEATAYVDP
                     DNEQKIQEALNVLTQDKTLIVIAHRLSTIQHADQIIVLGKQQILEKGSHHLLLKLNGN
                     YKKMWDTHMHTKDWGINTGHN"
     gene            20431..22164
                     /locus_tag="NBI57_000441"
     CDS             20431..22164
                     /locus_tag="NBI57_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCD1821424.1"
                     /translation="MFQITFKILNWIRPYKARMILGFSMSFLNAIFIALPIFLAAKIF
                     NNVLSHKPIYMKDILNVVIIMVLLVIGRFITAYFKSKSHESIAYEMSAKERLDIGDKL
                     KNVRLGYFNSHHSNELTTIVTTDLTFLENFAMKMVDIVVNGYILITVLILSLLVVSWQ
                     VSLLACIGVLLSFFAIQLLERKSRQNAPAYHNVQNQLVEKVLEVIRGIQVIKSFAKEN
                     TSLKSFNQAVNESKRINTKIEMQYIPFNLLHLLSLKVVSIMIVLVACLLYMNHSIDLP
                     TLIMISIFSFVIFDSVENINSAAHVLEMIDMTIDDIEKIKNAPELDENGKNLTIKNEN
                     IAFQNVNFSYDDKQVIKNVNFEIPTQTSTAIIGPSGSGKSTLCHLLLRFYDIDDGNIR
                     IDGVDIKDMTLSTLMSKISAVFQKVYLFNDTIENNILFGNPGATKEEIIRAAKQACCH
                     DFIMSLPEGYQTMLNEKGSNLSGGEKQRISIARAILKDAPIIILDEATASIDPENEQL
                     IQTAINELSKGKTVITIAHKLETIKNADQIIVLNEGEIIQKGSHDELIRKPGMYQDFI
                     RIKSKSAGWKL"
     gene            22395..22562
                     /locus_tag="NBI57_000442"
                     /pseudo
     CDS             22395..22562
                     /locus_tag="NBI57_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501179.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            22617..23303
                     /gene="bioD"
                     /locus_tag="NBI57_000443"
     CDS             22617..23303
                     /gene="bioD"
                     /locus_tag="NBI57_000443"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HCD1821425.1"
                     /translation="MRIFITSTNTDVGKTYVTKHLYHALKTRGHRVCVFKPFQTEERQ
                     DGTFPDLEVFKNECDLSYDITSLYTFKQPVSPHLAFKMTDQIFLNKQRVLDKVKVLDK
                     EFDFILIEGAGGIAVPIYEGTDDFYMTKDLINDCADCVISVLPSKLGAISDAVVHQDY
                     VNKNVSASNFLIMNRYTDSYIEKDNQITIGKLTNKTVYTFEEHATYENFSEAFLKQLI
                     GVKNELHTTT"
     gene            23281..24639
                     /gene="bioA"
                     /locus_tag="NBI57_000444"
     CDS             23281..24639
                     /gene="bioA"
                     /locus_tag="NBI57_000444"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HCD1821426.1"
                     /translation="MNYTQQLKQKDSEYVWHPFTQMGVYSKEEAIIIEKGKGSYLYDT
                     NGNKYLDGYASLWVNVHGHNNKYLNKVIKKQLNKIAHSTLLGSSNIPSIELAEKLIEI
                     TPSNLRKVFYSDTGSASVEIAIKMAYQYWKNIDREKYAKKNKFITLNHGYHGDTIGAV
                     SVGGIKTFHKIFKDLIFENIQVESPSFYRSNYDTENEIMTAILTNIEQILIERNDEIA
                     GFILEPLIQGATGLFVHPKGFLKEVEKLCKKYDVLLICDEVAVGFGRTGKMFACNHED
                     VQPDIMCLGKAITGGYLPLAATLTSQKIYNTFLSDSHGVNTFFHGHTYTGNQIVCTVA
                     LENIRLYEKRKLLSHIETTSSTLEKQLHALKRHRNVGDVRGRGLMFGVELVTDKDSKT
                     PLEIEKVERIVRKCKENGLMIRNLENVITFVPVLSMSNKEVKTMVRIFKKAVHNILDR
                     KC"
     gene            24641..25648
                     /gene="bioB"
                     /locus_tag="NBI57_000445"
     CDS             24641..25648
                     /gene="bioB"
                     /locus_tag="NBI57_000445"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HCD1821427.1"
                     /translation="MNLAKRILQGEQLTKETVLKIYEDTNIDTLDLLNEAYILRKHYF
                     GKKVKLNMILNAKSGICPENCGYCGQSRDIKQKQRYALIPEEQIIDGAKVAHDNHIGT
                     YCIVMSGRGPSDKEVDHISNTVRTIKSQHPQLKICACLGLTNDEQAKKLKSAGVDRYN
                     HNINTSENYHDNVVTTHSYKDRTDTIELMKANNISPCSGVICGMGESNQDIVDMAFAL
                     KEMDADSIPINFLHPIKGTKFGSMDDLTPMKCLRIVALFRLINPTKEIRIAGGREVNL
                     RSLQPLALKAANSIFVGDYLITGGQPNQLDYDMINDLGFEIDYDTCENKENKNDVSRA
                     N"
     gene            25626..26741
                     /locus_tag="NBI57_000446"
     CDS             25626..26741
                     /locus_tag="NBI57_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase
                     family protein"
                     /protein_id="HCD1821428.1"
                     /translation="MMFQEQIDLLQQKGLYRSLKSVGYIDRQYIEVENKRCTNYTSND
                     YLGLGQIAFDKDDFERFMRKYSYHLSSSRLISGSSTAYEEIETMLAGWLGYSACTILN
                     SGYDANLALFNIFKNTNCVVFSDQENHASIIDGIKLSGLEKVIYKHLDIADLEKRLEK
                     YPNQNIPKIIISDSVFSTNGDVVDIGQLVSLKHKYNATLILDVSHSFGIENYSNYQGV
                     DILTSSLSKACGAYGGVILSSNDVKDMLINHGRPLIYSSSLPIYNLYFIKRNIEKLIN
                     ADDRRTKLNSLSKYFNQKLKALNVNYNSSNSPIKFIEFDDIEAAENIHQTLLKHHVFT
                     SYLRYPTVTKPMLRISLSYFHTEQDIDRLFEILHQED"
     gene            26752..27444
                     /locus_tag="NBI57_000447"
     CDS             26752..27444
                     /locus_tag="NBI57_000447"
                     /EC_number="6.2.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxyhexanoate--CoA ligase"
                     /protein_id="HCD1821429.1"
                     /translation="MYSIKMRSSNQDVHISGAETICEFDKIEQTVQRFYNKGFFHENG
                     QPDFLNIKIQKIMEPIQQIKALQIIEDDKANLQHLTQECGVTEQALNQGMTYIKNETV
                     YTGAIILSAISGKRLDSFGQRGIRATHFSFEDINNKGDLNERVTDALAIASCINAHPY
                     VKGELCVSDDLTYTTGYFAAAKIGYHRLFDIKPVNTRYGGRIIFVDDCIDLNHYISFL
                     ESTPKQVVYETV"
     gene            27455..27922
                     /locus_tag="NBI57_000448"
     CDS             27455..27922
                     /locus_tag="NBI57_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HCD1821430.1"
                     /translation="MTSKDITQISVIAAILTILAVLKIPSIIPGLDFQLSAPAALLIL
                     AFFGIKKYFLGGLLSSVLLLIFGVFNPINVIISIIFRVIAIAVVYLLKINVLSLVLSS
                     VLGSLVSRLLLSIILNLPVWVVLLNAIPGVIFTLIVAIPLYLTLRKRMAVLLR"
     gene            complement(27974..28951)
                     /gene="hlgB"
                     /locus_tag="NBI57_000449"
     CDS             complement(27974..28951)
                     /gene="hlgB"
                     /locus_tag="NBI57_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bi-component gamma-hemolysin HlgAB/HlgCB subunit
                     B"
                     /protein_id="HCD1821431.1"
                     /translation="MKMNKLVKSSVATSMALLLLSGTANAEGKITPVSVKKVDDKVTL
                     YKTTATADSDKFKISQILTFNFIKDKSYDKDTLVLKATGNINSGFVKPNPNDYDFSKL
                     YWGAKYNVSISSQSNDSVNVVDYAPKNQNEEFQVQNTLGYTFGGDISISNGLSGGLNG
                     NTAFSETINYKQESYRTTLSRNTNYKNVGWGVEAHKIMNNGWGPYGRDSFHPTYGNEL
                     FLAGRQSSAYAGQNFIAQHQMPLLSRSNFNPEFLSVLSHRQDGAKKSKITVTYQREMD
                     LYQIRWNGFYWAGANYKNFKTRTFKSTYEIDWENHKVKLLDTKETENNK"
     gene            complement(28953..29900)
                     /gene="hlgC"
                     /locus_tag="NBI57_000450"
     CDS             complement(28953..29900)
                     /gene="hlgC"
                     /locus_tag="NBI57_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bi-component gamma-hemolysin HlgCB subunit C"
                     /protein_id="HCD1821432.1"
                     /translation="MLKNKILATTLSVSLLAPLANPLLENAKAANDTEDIGKGSDIEI
                     IKRTEDKTSNKWGVTQNIQFDFVKDKKYNKDALILKMQGFISSRTTYYNYKKTNHVKA
                     MRWPFQYNIGLKTNDKYVSLINYLPKNKIESTNVSQTLGYNIGGNFQSAPSLGGNGSF
                     NYSKSISYTQQNYVSEVEQQNSKSVLWGVKANSFATESGQKSAFDSDLFVGYKPHSKD
                     PRDYFVPDSELPPLLQSGFNPSFIATVSHEKGSSDTSEFEITYGRNMDVTHAIKRSTH
                     YGNSYLDGHRVHNAFVNRNYTVKYEVNWKTHEIKVKGQN"
BASE COUNT        10131 a         4307 c         5492 g        10008 t
ORIGIN      
        1 ttgtaaaaat gctaaaaaat gagatgtttt atttataatt ttctgcaaat ttatgatatt
       61 gtttcttaat atatcatatt aaaaatttgt ttttcttaaa cataggaggc ttatctaatt
      121 catggacaca tcaaaacaat ttagaggtga caaccgattg cttttgggta tcgttttagg
      181 ggttattacc ttttggctat tcgcgcagtc acttgttaat cttgttgtcc cattacaatc
      241 aacatatagt agtgacgttg gaacgataaa tatcgctgtt agcttatctg ccttatttgc
      301 tggtttgttt atcgtaggtg ctggtgatgt tgctgataaa tttggtcgcg tcaaaattac
      361 ttatgtagga ttgatattaa atgttgtagg ttcattactc atcatcatta cacctttgcc
      421 agcattttta attataggta gaataattca aggtttgtct gcagcatgta ttatgccatc
      481 cacacttgct attattaacg aatattatat tggtacaaga agacaacgtg ccttaagcta
      541 ttggtctatt ggttcttggg gtggtagtgg tatttgtacg ttgtttggtg gcttaatggc
      601 tacatatata ggttggcgtt caatatttgt tgtttcaatt ctattaacat tattagcaat
      661 gtacttaatc aaacatgcac ctgagactaa agcagaacca atcaaaggta tgaaagcaga
      721 agctaaaaag tttgacgtta ttggtttagt cattttagta gtgacgatgt taagtttaaa
      781 tgtaatcatc acacagacgt ctcattttgg tttagtttca ccgttaattc taggtttaat
      841 tgttgtgttt atctgttcat tagttggttt tgtatattac gaaaataaaa taaagcatcc
      901 acttgttgat ttttcaattt ttaaaaatag aggatacagt ggtgcaacta tttcaaactt
      961 cttattaaat ggtgtagcag gtggtgcact tatcgttatt aacacgtatt atcaacaaca
     1021 attaggattt aattcttcgc aaacgggtta tatttcatta acgtatttaa tagcagtgtt
     1081 gtcaatgatt cgtgtaggtg aaaagatttt atctcaacat ggtccgaagc gcccactatt
     1141 actaggaagt ggctttacag tgattgggtt aatcttattg tcgttaacat ttttaccaga
     1201 agtgtggtat atcatatcta gtatagttgg atatttattg tttggtactg gtttaggatt
     1261 atatgctaca ccatcaactg atacagcagt tgctagtgcg ccagatgata agtcgggtgt
     1321 tgcttcaggc gtatataaaa tggcgtcatc attaggaaat gcatttggag tagcagtatc
     1381 tggtacagtt tatactgtgt tagcagctaa tttaaatttg aacttaggtg gtttcacagg
     1441 tatgatgttt aatgccttgc tagcaattgt tgcattttta gtcattttac tattagttcc
     1501 taaaaatcaa acgaatttgt aaaaccgaaa tgaaagcaag ttattatgta gggattttaa
     1561 aggaaatttt gtgaaagtaa gtttatcata cacacttaat gttacgtatt gacgtttaat
     1621 gttaggtgtg ttcttttata gacgataaaa gctgtgtgca tattaagcga atgattttca
     1681 aattgacgct aatatgcgaa agtagtattt ttaaaatgaa caacaacgat gaagaggggt
     1741 ttataggatg aaaattgcaa ttgctggatc gggtgcatta ggtagtggct ttggtgccaa
     1801 actatttcaa gcaggatatg atgtcacact tattgacgga tatacatctc atgttgaagc
     1861 ggttaagcaa catggattaa atataacgat taatggagag gcattcgagt taaacattcc
     1921 gatgtatcat tttaatgatc aacgggacga aagcatttac gatgttgtct ttctatttcc
     1981 aaagtctatg caattaaaag aagtgatgga agatatgaag ccacatattg ataatgaaac
     2041 gatcgtcgta tgtacgatga atggtctgaa gcatgaagaa gtcattgcgc agtatgttgc
     2101 tcaatcacaa attgtcagag gtgttacgac ttggacggca ggtcttgaaa gccctggaca
     2161 cagtcattta cttggtagtg gaccagttga aataggtgaa ctagtggatg aaggtaaaga
     2221 aaatgttata aaagttgctg atttacttaa cgaagcggaa ttgaatggtg tcattagtaa
     2281 agatttatac caatcgattt ggaaaaagat ttgtgttaat ggtacggcaa atgcattaag
     2341 cacagtgttg gagtgtaata tggcatcact gaatgaaagt agttatgcga agtgtttgat
     2401 ttataaatta acgcaagaaa tagtgcatgt agcgacgatt gataatgttc atttaaatgt
     2461 tgatgaagta tttgaatatt tagttgattt aaatgaaaaa gttggtgcgc attatccatc
     2521 catgtatcaa gatttaattg ttaataatag aaaaactgaa attgattata ttaatggcgc
     2581 agttgcaaca ttgggtaaac aacgtcatat tgaagcgcca gtcaatcgct ttattactga
     2641 tttaattcat actaaagaaa gtcagcgaca cgcacaggat taattggtgt tttaggtatg
     2701 attacaagga aatgacaatc acgtgatatt acggtcatta ttaagattga aatgtaataa
     2761 ataaagaaca gcagtaaggt actttcaaat tgaaatgatc ttggtgctgt ttttcttgat
     2821 tgatcttcgt cataattcag atttgtcata ggtctacgac atactattag tatttactag
     2881 acagttttta cgacgacact ttgaaaaatt ttgaggcaaa tcatttggaa gtctcacgtg
     2941 aattttgtaa actcatcaag caagtaatta tattaaaaag acaaatagag aaaagatgtt
     3001 tataatgagt aaaatttttg taactggtgc aacgggcctt attggcatta aattagttca
     3061 aagactaaaa gaagaggggc atgaggttgc tggttttact acatctgaga atggtcaaca
     3121 aaagctagct gctattaatg taaaagcata tattggtgat atattaaaag ctgatactat
     3181 tgatcaagcg ttagcagatt ttaaaccaga aatcattatc aaccaaatta cggatttaaa
     3241 aaatgttgat atggcagcaa atacgaaagt acgtattgaa ggttctaaaa acttaattga
     3301 tgcggcgaaa aagcatgacg ttaagaaagt aattgcccaa agtattgcct ttatgtatga
     3361 acctggcgaa ggattagcaa atgaggaaac ttcacttgat tttaactcaa ctggtgatag
     3421 aaaagtaacg gttgatggtg tggttggtct agaagaagaa acggctcgta tggatgaata
     3481 cgttgtttta cgttttggtt ggttatatgg cccaggtact tggtacggaa aagatggcat
     3541 gatttataat caatttatgg atggtcaagt gacactttca gatggcgtaa catcatttgt
     3601 gcatcttgat gatgcagttg aaacatctat tcaagctatt cattttgaaa atggtatcta
     3661 taatgtagca gatgatgcac ctgttaaagg ttctgaattt gcagaatggt ataaagaaca
     3721 acttggtgtt gaaccaaata ttgatattca acctgcacaa ccatttgaac gtggcgtaag
     3781 caatgagaag tttaaagcgc aaggtggtac tctgatttat aaaacttgga aagacggcat
     3841 gaatccaatt aaataataat ttatccgttt aatatacaaa gaataaagac ttggtcgaat
     3901 cgtggatgat atattatcaa acgcacggct cgaacaagtc ttttttatta tgtcttcgtt
     3961 atctttgtat gaaggaataa cagaattaca attaatgtac tgaataatgc aattaatgtt
     4021 gtgattagtg ctaatttaat ttctattggt agccaagtca gtacaaaaga ccaattattg
     4081 ctaccgagaa tgagatatgg taatgcatat aatatgagcg ctatagcgat acataaacat
     4141 aaagttagcc aactcaatac agcaatccgt tttgatctac gcaaaagaat ttgaccatgg
     4201 cgcatttgcc aagcacgata agctgagaat ataaatgcta ataaaataag tgttgtcatc
     4261 aaaacggtaa caatattaaa ttggtcttta tattgattga gtatagacgc aaccgtcgaa
     4321 tatcgcttgt gatttacaat ttgtgtatta agatgctcaa ctaatttggg tacatattcc
     4381 gaatttagat ttgcaagcac aacaattcca taattttgtt ttggatttag taaaataaat
     4441 gatgaaaagt tatcgagcgt tcctgaatga aacactaaat gttcatcatt attggtaaac
     4501 cagcccgaag catatgcatt ggcattaggt tcaccaattg ttgatgataa atttttatgt
     4561 gattgttgaa ctaatgattt gtatttatca ggtggattaa gttggaattt tatccaatgt
     4621 tccaaatctt cagttgatgt catcatatat gctgatggtg tatcccaaag gttaaattca
     4681 ggtttagaga cgacaggtgt cgaaccttgt aattcgtagc caatagcatc atgttttgat
     4741 ttgttattgg tttgtttgaa tgatgtatgt gtcatatgca aaggcttgag ccatgaattt
     4801 gtaatatatt ttgtatagga ttgcttcgta acgttttgga taattaaacc taataaatca
     4861 tagttcatat ttgagtattc aaattcttct ccgggcttat gatgtaattc atcacccata
     4921 attgcacggg ttacatcatt taaacgatta tttttatttg ttacggcgtc ttcgcttgta
     4981 atatcactag gtataccact tgtttgagcc aaaagttgct taatcgtaat agtttcattt
     5041 tgaccattat agttcatttt aaaatgaggc acatgtttgg atacgtcatc atttaagttt
     5101 aatcgacctt cttgagctaa ttttaaaatt gcaagacctg tgaaagcttt agtattagaa
     5161 gcaatttcat actttgttgt gggagaagct ttgacttttt tatcaacatc ttgataacca
     5221 taacctttat ttaaaaaaac tttgccattt tttactatta aaattgatgc tccaggaatg
     5281 tgtccctttt gtaaatcatg ctcgataatt ttatctattt gttgttgcga atcattggtt
     5341 aaccgtgtct tcgtattgct atttaatgtt atatatatag caattgaaat ggcgactaaa
     5401 atgataatag aaatggatag aaaatacagt tttttagtag tcataaacgt tcctttttat
     5461 atgttaaatt tacttaagtg cctaatgaga tttattttat tataccagtt ctattgaata
     5521 aattaattaa agaggtatat ttaccattat ttttgacacc ttatatgtta ataggtgtat
     5581 aattacataa aaaatattaa ttaactctct atactttttg gaaagatagg cgtatagtga
     5641 tatccatcaa aaactataaa aaggaatagg agattatgtt caatcaattt aaaagactta
     5701 ttatagggca acctaaaaaa aacagagaac taaaagacga aaagatatcg aagtttaaag
     5761 ggttagcgat tctttcgtcg gatgcattat cttcagtggc ttacggtccc gaacaaatac
     5821 tgattacatt atcagtagtg ggtgcagttg caacttggta tactttaccg attgcaggtg
     5881 ctgttctgat cttattagct gctttaatta tgtcttatag gcaaattatt tatgcatatc
     5941 ctaaaggtgg cggcgcgtat atggtgtcca agaccaattt aggagaaaaa tggggattgc
     6001 ttgcaggagg ctcattatta gtcgattaca tactgactgt cgctgttagt atatcatctg
     6061 gtgctgatgc atttgttgct gcattcccaa gtttatatgg gcataaagta ctgattgcat
     6121 gtttacttgt actttttatt ttaatattaa atttacgtgg tttaactgaa tctgccaccg
     6181 tattatccta tccagtatat cttttcatta tagggttagt gatattaata tttataggta
     6241 ctttccgtgt ggcgacagga gatattcaac cacatatgca tgcatcagta ggaactgcgg
     6301 ttcctggagt aacattattc ttgctattaa aagctttttc atcaggggct tcatcattaa
     6361 caggtgttga agctatttca aatgcggtaa ccaattttag agaaccaagt gctaataatg
     6421 ctgtaaaaac gttaattgct atgggttcaa ttttagcgtt tttattagtt ggtatcgttg
     6481 gtttagcata tgtttatgga attatgccac aaacggaaac gacagtttta tcacaattag
     6541 ctatgcaaat atttggtgat aatgctgcct tctattttgt acaggcaacg accgtgatga
     6601 ttttggtatt agcagccaac actggattta cagcttttcc gatgttagcg gcttcaatgt
     6661 ctaaagataa atatatgcct agaatgttta ctgttagagg tgatcgctta ggctactcta
     6721 actcaattat tattttaggt gtattagcca ttattttaat tattgtattt gatggtatga
     6781 ctgaagattt aattccattg tatgctgttg gtgtatttat tccatttaca ctagcacaat
     6841 tcggtatggt tattaaatgg attcatgaac gtcctaagaa ttggttaagt aagttgtctg
     6901 tgaacttact aggtggtatc gtgacattta ttgtttttat gattttactc ataactaaat
     6961 ttagccaagt atggccgata cttattttct tgccgttcgt ggtgattttc ttcttgaaaa
     7021 ttaacaaaca ttatcgagat atcgcagaac aattacgttc tgacattgat gttcttaatg
     7081 tggacgtggt agatcgtaat ttagcaattg tgccgataac aagtattaca acagcagttg
     7141 ataagtctat ttattacgca caaatgcttg cgaataatga tgtcatagca gtacatgtat
     7201 catttggaga tgaagatgaa aaagcattcc aagagaaatg gaaacgtcat ttcccagatg
     7261 taagattagt tattttacat tcggaatatc gcagtattat acgaccaatt tcacgtttta
     7321 tcgataagat taatcgtaaa gcaaacgatc aaaactatat gattacagta gtgataccag
     7381 aatttattac taagaaacgt tggcataatt tattacacaa tcaaactagt ttgcgtatga
     7441 aattgtattt aatttatcaa aagaatgtta acgtatgtac aattccattt aaacttaaaa
     7501 aatagattct ataatcaaat cggattgatg aaaggttatt tacttttcat caatccgatt
     7561 ttttgatgtt gaatataaaa accccgtaca tatgagatga taatgacttt gtctcatatg
     7621 tatggggggt attgttatgt cttgcttaat gtttggactg taaagacaag acaatttcgt
     7681 tgtaattgat attttctcga atttgagctg caacgatatt gttgatttta tttttgcgaa
     7741 ttaattcatc taaaaggtga cgttgtaagt acaatgcttc caatttgtgg ttatggaaca
     7801 tagcttgcgg tatgatttga ttgctgtttt cttgacgttc ttttcgacgt tgtgcacgcc
     7861 actttaatga gcgactcata tcatagtaat gattaatgac taagctaacc tcaagtacat
     7921 ttgtactatt ttgttcttct tttaagcgta aaataatatt gtgattcaca atacgcataa
     7981 ttttttgaac atctttgaat gaattttcaa attcttgctt cttagattct ttgtttaagg
     8041 ctttgtagtg cttcatcgct ttaagtctaa gccataacat cttgaagaag tttgcaaatt
     8101 tttcaacaaa tgatgcagta cggtgtgtct ctgttaattc gacaatattg cggtaattat
     8161 taatatcctg atacgttgcc ttacctttat caattaaacg ctcaagtgta gatgtttcga
     8221 ttacttttgc aatatcttct aaacgtttga gttcttttgt attttgatta ttaggttctg
     8281 aatttaataa aaataacaat tctccatagt attggtttaa tactgggcga taatttgtat
     8341 cgtttttgtc ttttttactt tcgtttttaa aatgctgtat cactttttga acaatgaaaa
     8401 ttttggcaga ttgataagtc atacctttaa aagtagtatc ttcggcagat ggtgtaatta
     8461 aaggtaaaac aatttgcgct aagattaaac taattaatac catgaaagat gcaataaaca
     8521 ataaatcatt acggtattcg aatgcttgtc cttttgtaat gataaatggt aatgtaagtg
     8581 ccattgaaag tgaaattgta ccgtgaatac cacacatcgt cataataaat gcgtaacgca
     8641 cacgagaagg tggtgtttca tgtgaatcat gttcttcgtc tagataagat tgtatatttt
     8701 tcgggaaata aaaatctttg taccagaaat aaacccaaac aaatcgacat gcataaattg
     8761 caatagcgat taatatagtg atcactatta aaaatgaaaa gttgtcaggc tctgtttgga
     8821 aaatatctat gactacagta ggaatcataa aacctaatac aacaaataca aaaccattta
     8881 aggcataact aaatgtgttc caaatttgat ggtaattcat ttgtagttcc gtttgtgctc
     8941 taattaaacg gtcgcgttct aaaccatgga ttagacctgc gattacaact gcaatgatac
     9001 ctgaagcatg aacttcttct gctaaaaagt atacgacaaa aggagttaat aattgaataa
     9061 aagttaaggt attgttatct tttaaacctt tattagcggt taagtctata cgtattctaa
     9121 cgacaacgaa tccaataatt gcaccaataa gtacacctag tattgttgaa atgataaatt
     9181 gttcaacagc ttggaataat gaaaaggtac cagttactaa tgcagtaaca gcaattttaa
     9241 atgaaatgat acctgctgca tcattgagta aagattcacc ttctaaaatc gtcatagaac
     9301 ctttaggtaa taattttccg cgtgtaatag cagatactgc tactgcatca gtaggacata
     9361 aaattgctgc tattgcaaaa gcggctggca ttggtaaggc aggccaaatc caatgtataa
     9421 aatagccaac accgactaca gttgcaaaca ctagtgccat tgacattaat agtataggtt
     9481 tacgatattc taataatttt gttcgagaga cgtgggtacc ttctacaaaa agtagtggcg
     9541 cgataacggc aaacataaac acttcagaat tgaattggaa atcaacttgt attggaataa
     9601 tgaaaataac gacacctaat gcaatttgaa taaaggcagt aggaatttgt gggaatcgat
     9661 tattgataac cgaactaata atcacagcaa aaataaaaat taaaaatgct tctaatagtg
     9721 ccatacaata ctcctcaaaa ttttaatagt taatatttta tcacttttaa ggcataatga
     9781 catagatata ttgataaaat gaagttattt tcaaaaaaac tctagtatcg gttgaactga
     9841 tactagagcg agatgtttaa attattgatt gtcatatctg aaatgaccgc tgtcattttg
     9901 tcgttgttca tacgcgagct tttcagcatt cgttttgtat tttttataaa agaaaaataa
     9961 aaatataaac cagaatggtg aaatataaat agctgctctt gtttcgtcac taaagaataa
    10021 taaaatgaat acaaagaaga agaacgctag aataatgtaa gcaataggct taccaccaat
    10081 caacttaaat ttactgtttt tatgtgcctc aggatgcttt ttcaaataca ttagataagc
    10141 gacaataatc attgcccaaa caactaaaaa caacacagtt gataacgttg taacgtatat
    10201 gaatagttga attgcattag ggaaaatgta gtttaacaag gctgctataa gtaatgcaat
    10261 tgatgaaact aacattgata aataaggcac gccatgacta ttcgttttat ttaaaacttt
    10321 aggacctaac ccttgttgtg acagtccgaa taagatacga ctattcgaat agataccact
    10381 atttgtagca gaggccgcgg cagttagcac gacaaagtta acgacgcctg ctgcaaatgg
    10441 cacgccgatt aatgtaaaga gtttaacgaa agggctacta tttggatcga tatcattcca
    10501 aggtatgact gacataatta ctaatagacc accgatatag aataataaaa tacggatagg
    10561 tacattatta attgctttcg gtaaggtttt ttcaggatct ttcgtttcac cagcagttac
    10621 acctataagt tcaataccaa tgaatgaata tacagcaatt tggaatgaca ttaggaaacc
    10681 aaatgttcct ccagggaaca tgccgccgtg actgattaag tttgtgaatg atgcatgtcc
    10741 ataatgtgtt ttaaatgaga aaaagattaa tacaagacca acgataacca tcgcaataat
    10801 tgtgacaact ttaataatag agaaccaaaa ctccagttca ccaaataatc tggcacctaa
    10861 taagttgaag ctaatcaaga ttaaaacaat aaatagcacg gtaatccaat ttgggacttg
    10921 tggataccaa aatgcaaagt attgtcccat cgcagttagg tctgacatac ttgatacaat
    10981 ccaacataac cagtaagtcc agccaatgac aaagccacca aaagggccta aatattcatt
    11041 tgcaatatca acaaatgaat taaatcttgt attgctcaac aacaattcgc ctaatgcgcg
    11101 cataaaagcg aatagtacaa ccccaataat catgtatgtg aataacagtg atggaccagt
    11161 taaagatatt gtttgacctg atcctaggaa taacccagtt ccaattgcgc ccccaattgc
    11221 tatcaattgg atgtgtctgt tattcaactc tctatgcaat tttcttgcca taattttcct
    11281 cctcaggata aagcaaataa aaaagttgtg tgtaagcgtt ttcttaatta taaatactaa
    11341 agaaatccat ttaataaagc aattattttt ttaaatataa gaaaaaacat ttgataggta
    11401 agggtatgat aactaaaact tatcgatttt ttgaaaagat ataagttttt atgcaaaaat
    11461 ataaaataat catctaaatg aagcagttaa ctatatatat tagataagct atggcataat
    11521 aaataacatt ttaatttgag taaagaaggt atgggtatga agatagggac aatcgccgat
    11581 ttacatattg atcgtcataa taaaaaaaca tccgaagatt acttagaggc attggttgaa
    11641 atagttaagt ataagaaatt ggatatattg ttgattgcgg gagatatctc aaatcattat
    11701 caattgacgc accaatttat aacgcaattg acaaaacaat tagatatacc tgtgaaattt
    11761 gttcctggaa accatgattt atgggaagtt gaaagtatga ctacgcaaga catttggaat
    11821 aattataaga gcatgtcaca gtgcttggta ggaaaaccat ttatagtaaa tgaagaatgg
    11881 gcaataatag gacatacagg ttggtatgat tatagctttg cagcacaacg attttcatta
    11941 gaacatttac aaaaaggaaa acattatggt gcgacttggc aagataaaga acgaatatct
    12001 tggggcatat cagatcaaaa tttatctaaa atagcggctg aacaagtgaa gaaagatata
    12061 ttagaagtag gaaatagacg agtgatttta gtcacacatg ttgtgacgca ccctgatttc
    12121 attgttccta tgccacatcg tatattcgat ttttataatg catttattgg gacaagtgat
    12181 ttcaatcctt tgtatgcgat gttcgatata ccatatagta ttatgggcca tgttcatttt
    12241 cgtaaaagtg tgatggatga tggcagatgt tatctctgtc cgtgtctagg ctatccaaga
    12301 caatggcgtt cagaagatat ttaccaggaa ataaatgaga cgatacaaat aatagaaatt
    12361 taaaatgcgc aaacctgacc cagtttgcgc attttatgtt ttacacacgc gagtaatgtg
    12421 tttacttacg tgtgtttatt ttgttgttga ttttcaattg tatatgaatg tggttgcaca
    12481 taaatgcatt ttcttcctgg tgaattaaag ctgtattcca ttttctcttt acgaatttta
    12541 ataatttgtt tgcgatttgg aatgatggca ggtaaaacta ggccacgacg aatatgactc
    12601 caaagtggat cgatataata gtaatagtaa tcatcgtacc caatgagtaa cgttacgtga
    12661 atatttgaaa cttgtttcgc tggcttgtta tcaaagcgga aaacacgtag taatggttta
    12721 gagccaagat tagtatggta tattaacaca ggttgacctt gatcgataat acctttaaga
    12781 tcttctaagg atttaccagt gccgtctacg atattaggat tgtatttttg taaaaatggt
    12841 acatatgctt ctggaaatat cgtttgatga taattgccaa gcttaatgaa taagtgatgt
    12901 ccaacataac ctttatgtgg attgttcgga tgtgtcggcc aatgtctcat aatttctgta
    12961 gcagggatat gttggttatt gtattgcaac atcatggctg cggaaacacc ttcacacccc
    13021 atgaccatag ggataggaaa tagctgactg ataggtttaa ctggtaatat ttttcggttc
    13081 ataatatagt cctcgcattg attcaataaa tatttaatat aattatatag cgtcaatgca
    13141 aaatgtccta aacatatgtt ttacatgagt gaataaaatt aatggagtga taaaatggaa
    13201 tatcaattac aacaacttgc gtcgttaacg ttagtaggta ttaaagaaac gtatgaaaat
    13261 ggacgacagg ctcagcaaca tatagccggg ttttggcaaa ggtgttatca agagggagta
    13321 attgcggatt tacagttaaa aaataatggt gatttagccg ggatacttgg cttatgtata
    13381 cccgaattag acggtaagat gtcatatatg attgcagtta ccggagataa tagtgctgat
    13441 attgcaaaat atgatgtcat aacattagca agttcaaagt atatggtatt tgaagcacag
    13501 ggcgcagtac ctaaagcagt tcaacaaaaa atggaagagg ttcatcacta catacatcaa
    13561 tatcaagcaa atacggtaaa atcagcacca ttttttgagt tgtatcagga tggtgatact
    13621 acaagtgaaa aatatattac agaaatttgg atgcctgtta aagggtgatt gaatagaaat
    13681 gtacttgata tgaaggcgta attttagttg aagattggat ttcataattc ataaagaaga
    13741 accccgacaa agatagatat ctttgctagg gttctaatta tgttgaagtg ttatgcgaca
    13801 tgttgctttt taatcatttt aaagtaaatg atttgtagac tgactaatag aatggctgta
    13861 atgaagataa taatcatata tggtgatgta ttaaattcgc cttttaagcc aactaatggt
    13921 gcaacagcgc cacctaagat aaattggaat aagccaagta aacttgatgc gttaccactg
    13981 ccacctgttc gttcttccat agccattgtg aaaccaagtg gtccaattga cgtcacagga
    14041 cacacattta agaagaatgc gatgagtaag acccaaagtg gtaaatggaa tgtaagtgtc
    14101 aggataatta aagcaacacc caccacttgt ataatagtta aaatgattaa taatatgtgg
    14161 cgatgtaatt tttctactaa taaagcaacg acttgactga caatgattaa acctacaccg
    14221 ttaacagcaa acatgatact aaattgttgg ggtgtcatat tatacaattt ttgagtaata
    14281 aatggcgatg cagatgaata actaaaaagc attacataag ttaaaccttg taatagcatt
    14341 ggaataataa atgctggttt tttcaaaaga cttccaaagt ctttaatgac gctactaaaa
    14401 ttcacctgct ttaattcatc tttagatgtt ttaggtaatt gagaagcgac gccaattaaa
    14461 atgatgagtg ccacaatagt taaaattgtg aaaatagaac gccatgttgc tacggataaa
    14521 gctaatccac cagctaatgg tgcaagaata gtgatgatgc catttactac cattaaagat
    14581 gctaaaaatt tagcgagtgc gttgccacta aatttatcac cagcagaggc tttggcaatc
    14641 acgatgacgc caccaccagt taaaccttga ataaatcgta aagttaagaa taatggcaat
    14701 tgatcaacaa acatagacaa acctgatacc aaaatgaaaa tgatcaaaat ggatacagca
    14761 atccgtttgc gaccaaaagc atcggataaa ggtccaaaga taaattggcc tagtgcaaga
    14821 ccaatcattg tgaatgataa tgttaattga atttctgatg tagtagaacc aaaatcacct
    14881 tgcactttag gtaatgatgg tacgtacata tctatagtta gtgggccaaa agcggtcatg
    14941 acgcctaacg taattagcaa taaaaatgaa aaatgcttcg ttttggtttt cgttgattgc
    15001 atgatcagac tcctttaaat gatataatta tataaattat atacgttttg tgtgtatttg
    15061 tatgtagtta gaatacaagt ttatataaaa aatccctata ctcttaatgc atcatcacga
    15121 ataacgtagt gatacagata aagtataggg aattaatgtt tatcatcaaa gcaggtgtaa
    15181 taattaatta gcgccaccat tgtcatcttt aaataagctt tctaatttag atttaatact
    15241 gtcgaatttt gtattaatat catttaacat gctttcaatt ttaccttggc ttgcattgtt
    15301 tttaagtgca ttatttgcct ctgtcaattt gttgatttgg tcttgataaa attgagttag
    15361 cttagaatca gctttgtttt gttcttgctg cttcaagttg tcgatttgat tttgtaaatt
    15421 tttaataatg tcatcagttt tttggttgtt tttaatattt tctttagcat tatctacttg
    15481 ctggttaatt tggccgtctt gattttcaac ttgtttttta taattttgag agactttagg
    15541 gtcattacta actttatcgc tatcattgtt aagtaaggcg cgtgtgacaa aaatagcgac
    15601 aataattaag agaatggcaa taatgatata taaccatttt tttgaacggc gggatttctc
    15661 gttttcttct tcaagaattt gttctcttcg ataatctcta ccattatagt atcgttccgg
    15721 gtgttcttca ggatcgcctt tagcatattg ttgtctatac gatgcgtctt cagattgacg
    15781 acgatgattt tggttttgat tgtcgtattg atatgaatca cggtatttat cagttctttt
    15841 catgggtttc acctcattca agttttaact gcaaataata aagctgatga tttactttga
    15901 cattattgta atttcagaat acgagcaatg tcagttgcag tgtttaataa attttgttcg
    15961 ctattttgta ggacatcttc taaagtgcta ggtgaagaga ttatagaata ggcactatca
    16021 ataccgaaat cgtaaacatg ttgataattt tcgcctagac tgccacaaat cgcgatgaca
    16081 ggaatatgat attgttttgc agctaacgca acgcctacgg gtgttttacc aaagatggtc
    16141 tgataatcca tgcgtccttc tccagtaata acgaggtctg catctttaat tctttgatga
    16201 aaatctgtaa tgtcaaagac gacatcaata ccttttgtta aagttgtctc acaaaacgct
    16261 aataatgctg cgcccatacc gccagctgca ccagaacctg gtatttgatt aacggacttt
    16321 cctgtgcaca tttttatctt atcatgataa tgcgacattg cgaaatccaa ctttggtatc
    16381 atctttgcat cagcgccttt ttgaggacca taaatatagg tagcaccatt ttcacccaat
    16441 aaaggatttg aaacatcaca ggccacttta aaggtcacct cttttaatcg cgaatctaga
    16501 ttggttatat cgatttgtgc aatgtgagca agattagcac cattcatttg taataagtcc
    16561 ccgtttacat cagtaaactt tacgcctagt gcacttagca tacctgtacc accatcattt
    16621 gttgcactgc caccaatccc taaaataatg gtcttagcac catgatttaa tgcatcttta
    16681 attagttcac cggtaccata tgatgatgtg tataaaggat tacgttcctc tttttctaat
    16741 aaatccaaac ctgacgctgc cgccatttca ataattgcaa tttgttgttc gtctgcgcgt
    16801 gcataacatg cttcaatagg tcgcattaaa gggtcattaa cgatgactgt atacttagtg
    16861 gcacctgttg catgcattaa agcatctgtg gtaccttcac caccatcagc catcggaatg
    16921 atatcataat gaagggtatt cccataaaca ttagtaaatg cctgttttat aatattgcca
    16981 acttgctgtg cggtcatact ttccttaaat gagtcaggag cgatgacaat tttttgtaat
    17041 tgcatagtag acatcctctc attactgttg tcgctcttat tgtatataaa attactagtg
    17101 aactaaagta atctctatag tgctaatgag tcacgcgata aatcagttac attataatgg
    17161 cgtataagga tgagaggaaa gagactaaat ttgctgtgaa agggtataaa ggttgaaata
    17221 agtaaatttg aagtgaagcg gattggctcg catattcgag tatgttggag aacgtagcta
    17281 tgcatatatt ttgatgttga ggagttacac atatgaaact aacacaaacg catgctgaaa
    17341 ttttaaaatt tatcattgtt ggcggcatta atacgttaaa ttattatgtt gtctatttat
    17401 tgctgttaaa attattacac attgaatata tgattagtca tattaccgga tttctagtcg
    17461 cttttgtgat ttcgtattat ttgaattgtt attttgttta cagggtaaaa cctacttgga
    17521 gaaaattcat tagttttcca attacgcaga ttgtcaacgt aagcttacaa acagttttat
    17581 tatatgtttt tgtatcctgg ctaaatttgc cagcggaaat tgcgccattt gcaggtttag
    17641 tgattacgat acccatcaca tttgtattat ctaaatggat tttaaaagat agtaattaaa
    17701 gtagcgtaaa ccactaaaaa acgctttact cgaactttaa tattgcgtga aatataataa
    17761 ataaatgata taatataatg gtcgcctaac atctcgggcg ttactttaac gcagagagga
    17821 ggtgtatgat ggatgttcaa tttattaatt aacatcatga cttcagcttt aagcggctgt
    17881 cttgttgcgt tttttgcaca ttggttacga acgcgcaaca ataaaaaagg tgacaaataa
    17941 ccaacaccta aataaatccc ctcactatgg cagtagtgag gggatttggt gtatttagat
    18001 gttcaattta ttatgtaatt aattatagca ttgataaaca aggtgtgcaa tgtattttgc
    18061 aatattacaa taaatgaatt taattatatt agaattaagt atttcgaaaa atgatttaat
    18121 agttgagtca taggatcatt agataaagcg aataatcaat tatagggaca ttgggataca
    18181 aatcaatgta ctagactcta caaggctata ctcagggacc caacatagag aatttggatc
    18241 cacagtttct acagacaatg caagttggcg gggccccaac acagtagctg gcggaaagtc
    18301 agcttacaat aatgtgcaag ttggcggagc cccaacatag agttttactc atgtattcct
    18361 atttttaagt acatattagc tgtggctaag gataaagaat cattacataa taaatcatta
    18421 gtggctcttt atcatttctg ccccataacc ttgtgaattt ttaaaagaaa tgtgtcacaa
    18481 atccacgcga cattgataat cgttatcaac taatatatga tgtacttgta ataagtatta
    18541 atttttaaca aatgacaaat ttatattatg tatatcatcc agtgaaataa ataatataag
    18601 cggcttaatt tagattatta attgaaagga gttgcatata cgatgaacaa tcagacaaat
    18661 tggataaaga tactcagcgg atttgcttct gatagtaaat ggaaaattat gttatcaatt
    18721 ttgttgtcta tcatcagtgt cttttctgga ttagttccat attgggcagt ctttaaaatt
    18781 attttaatga tgattaacaa tgcatatacg attaattcga ttatggttta tatctttatt
    18841 gctttaatca cttatatttc acaagtgtgt tgttttggag cgtcaacgat gctatcacat
    18901 attacggcat atgaaatttt atctgaaatt cgtaaaaaat tagctcaaaa attaatgcgc
    18961 ctccctttag gcgtagtgga atctaagaaa ataggtgaat taaaaaatat atttgtcgat
    19021 aaggttgaaa caatagaatt acctttagca catatgattc ctgaagttat aggaaactta
    19081 cttttgtcag ctgctatctt tttatacata atgctcattg attggcgtat ggctagtgcc
    19141 ttattagtaa cgataccaat ttcaattttc gcttttaaaa aagttatgtc tggatttaat
    19201 gagacatatg ctgaacaaat gaaatcaaat aattatatga atagcgcgat tgtagagttt
    19261 atcgaaggca ttgaagtgat taaaacattt aatcaatctc aaagttcata taaaaaatat
    19321 aaagatgcag tagataatta taagattcac actttgaatt ggtttaaaaa tacatggggg
    19381 tatatgaatt taggagctag tgtattacct tcaacatttt tagggatttt accggtcggc
    19441 atgtatttga tatctatcaa ccaattaaac tatgcggaat ttttcctatg catcgtatta
    19501 tctttaggtg tagtagcacc gattaagaat tttactaatt atgtaaatca tttaaagtct
    19561 atacaatacg cattaactga agtgaatcaa atattaagtc tagaagagtt agtattgtca
    19621 acaaaattta agaagcctca acattatgaa attgctttta ataatgttgg attttcatat
    19681 aacaaagata aggacgatct ggtctttaag catttatcat ttacagtacc agaaaataat
    19741 ttcacagcta tcgtcggggc ttctggtagc ggtaagtcaa ccattgccaa gcttatatca
    19801 cgatactggg atgtgacttc aggtgaaatt acgattggcg gtataaatat aaaagatatt
    19861 gaatcgaaac aacttaacga tttagttggt tttgttggac aagataactt tttattaaat
    19921 cttactttta aagaaaatat taaacttggc aatccagaag ctacggatga ggcagttgaa
    19981 aaagctgcca agttagcaca atgtcatgaa tttattgaaa agttgccaga tggatatgat
    20041 acgaatgttg gtacagtggg agataaattg tctggtggtg aaaaacaacg agtcactatt
    20101 gcaagaatga tattaaaaga tgcgcctatc attgtattag atgaagcgac tgcttacgtt
    20161 gatccagaca acgaacaaaa aattcaagaa gcattaaatg tgttgacgca ggataaaaca
    20221 ttgattgtta ttgcacatcg gttatctacg attcaacatg cagatcaaat tattgtttta
    20281 ggtaaacaac aaattttaga aaaagggtca catcacttat tgctaaaatt aaacggtaat
    20341 tataagaaaa tgtgggatac gcacatgcat acaaaagatt ggggaatcaa tactggacat
    20401 aattaaacta atgagaaaag gaggtcaaat atgtttcaaa ttacatttaa aattttaaac
    20461 tggataagac catataaagc aagaatgatt ttaggattta gcatgtcatt tttaaatgct
    20521 atttttattg cgttacctat ctttttagct gcaaaaatat ttaataatgt gctatctcat
    20581 aaacctattt atatgaaaga tatattgaat gttgtaatta tcatggtttt attagtgatt
    20641 ggacgattta ttacagcata tttcaaaagc aaaagccatg agagcatcgc ttatgaaatg
    20701 agtgcgaaag aacgtttaga tataggggat aaattgaaaa atgtaaggtt aggttatttt
    20761 aattcgcatc attcaaatga gttaacaaca atagtaacaa cagatttaac atttctagaa
    20821 aactttgcca tgaaaatggt agatattgtt gttaatggat acatattaat tacagtactc
    20881 atattgtctc tacttgtagt ttcgtggcaa gtatcattat tagcatgcat tggcgtatta
    20941 ctatcatttt ttgcgattca attattagaa agaaagagtc gacaaaatgc gccagcgtat
    21001 cataatgtac aaaaccaatt agtggaaaag gtattggagg ttattcgtgg tattcaagta
    21061 ataaaatcat tcgcgaaaga aaatacgagt cttaaaagtt ttaaccaagc agtcaatgaa
    21121 agtaaacgta taaatacaaa aatagaaatg caatatatcc cattcaattt attgcattta
    21181 cttagtttaa aagttgtttc aataatgatt gtattagttg catgcttatt atatatgaat
    21241 catagtattg atttacctac ccttattatg atttcaattt tttcatttgt gatatttgat
    21301 agtgttgaaa atattaatag tgctgcacac gtacttgaaa tgatagatat gacgatagat
    21361 gatattgaaa agataaaaaa tgctccagaa ctggatgaga atggaaaaaa tttgacgatt
    21421 aaaaatgaaa atatcgcttt tcaaaacgtg aacttttcat atgatgataa acaagtgata
    21481 aagaatgtga attttgagat acctacacaa acatcaacag caataattgg accttcagga
    21541 agtgggaaat ctacattatg tcacttactc ttgcgctttt atgatatcga tgatggaaat
    21601 attcgcatcg atggtgttga tattaaagat atgacattaa gtacgttaat gtcgaaaatt
    21661 agtgcagtat ttcaaaaggt gtatttattt aatgatacga ttgaaaataa catattgttt
    21721 ggcaatccag gtgcaacgaa agaagaaatt attcgtgcag cgaagcaagc atgttgccac
    21781 gacttcatca tgtcattacc tgagggatat caaacaatgc taaatgaaaa aggtagtaat
    21841 ttatctggcg gagaaaagca aaggatttct attgctaggg cgatattaaa agatgcacca
    21901 ataattattt tagatgaagc aactgcaagt attgaccctg aaaatgaaca gctgattcaa
    21961 acggcaatta atgaattaag taaaggcaaa acagtaatta caattgcaca taaacttgaa
    22021 actattaaaa atgcagatca gattatagtg ctcaatgaag gtgaaataat tcaaaaaggt
    22081 agtcatgatg aattaattcg aaaaccagga atgtatcaag actttataag aataaaaagt
    22141 aaatcagcag gctggaaatt ataaataata gtttaatatg gaatcagaat ttggaaataa
    22201 aaagggtgat agataatcaa cagcaaaata aatcccctca ctagggcaat agtgagggga
    22261 tttggtatat ttagatgttc aatttattat gtaactaatt atagcattga tgaacaaggc
    22321 gctcaacgta atattacaat taatggattg tttcacaaca tggtaaagct aggatgtctt
    22381 tttgttaaga aataatgcaa ttattaattt taactgacat attttaacga atcgctgttt
    22441 atatctgccc aaatttcgta agaagcctta gtcatttcaa tagcaaggtc aaaattttgc
    22501 caaaaattat agttatactg agaagtttaa attcatgttt tcttgaaaaa catgcgcctt
    22561 aaatgtaaac ttattaatta taaaagttta cattcggatt gaggtgctta ttttttatga
    22621 ggatttttat tacaagtacg aatactgatg taggcaaaac ctatgttaca aagcatttat
    22681 accatgcttt gaaaacacgt ggtcatcgtg tttgtgtttt taaaccattt caaactgagg
    22741 aacgccaaga cgggacattt ccagatttag aagtatttaa aaatgaatgt gatttaagct
    22801 atgacataac gtcactttat acttttaagc aacctgtatc accacacctt gcatttaaaa
    22861 tgacagatca aatttttcta aataagcagc gtgtattaga taaggtaaaa gttttagata
    22921 aggaatttga ttttatctta attgagggtg ctgggggaat tgccgtacca atatatgaag
    22981 gtacagatga tttctacatg actaaagatc taatcaatga ttgtgcagat tgtgtcatca
    23041 gtgtgttgcc atcaaaatta ggtgctatta gcgatgccgt tgttcaccaa gattatgtta
    23101 ataagaatgt atcggcgagt aattttttaa taatgaatcg ctatacagac agctatattg
    23161 aaaaagacaa tcaaattacg attggaaaat taacaaataa aacagtctat acatttgaag
    23221 aacatgccac gtatgaaaat ttctcagaag catttttaaa acaattaata ggagttaaaa
    23281 atgaattaca cacaacaact taaacaaaaa gactcagaat atgtttggca tccatttaca
    23341 caaatgggtg tatatagcaa agaagaagca atcatcattg aaaaaggaaa gggtagttac
    23401 ctttacgata cgaatggcaa taaatattta gatggttatg catcgttgtg ggtcaatgtg
    23461 catggtcata ataacaaata cttgaataag gtaattaaaa agcaactcaa taaaattgcc
    23521 cattctacgc tgctaggatc atcaaatatt ccgtcaatag aacttgcgga aaaattaatc
    23581 gaaatcacgc caagtaatct aagaaaagta ttttattctg atacaggcag tgcgtctgtt
    23641 gaaatcgcaa taaagatggc atatcagtat tggaaaaata ttgatagaga aaaatatgcc
    23701 aagaaaaaca agtttataac gctaaatcac ggttatcatg gggatacgat tggtgcggta
    23761 agtgttggtg gtatcaagac ctttcataaa atatttaaag acttaatatt tgagaatatt
    23821 caagtagaaa gcccatcttt ctatcgcagt aattacgata ctgaaaatga aattatgaca
    23881 gctattttaa cgaatataga gcaaattcta attgaaagaa atgatgaaat cgcagggttt
    23941 atattggaac cgttgattca aggtgcgaca ggcttgtttg ttcatcctaa aggctttttg
    24001 aaagaagtcg agaaattgtg caaaaaatac gatgtcttat taatttgtga tgaggtagca
    24061 gttggttttg ggagaactgg aaagatgttt gcatgcaatc atgaagatgt tcaaccggat
    24121 attatgtgtt taggtaaggc gattactggt ggctacttac cacttgcagc tacattgaca
    24181 tctcaaaaaa tatacaatac atttttaagt gattcgcatg gtgttaatac ctttttccat
    24241 ggtcatacat acaccggaaa tcaaatcgtt tgtacggttg cattagaaaa tataagactt
    24301 tatgaaaaac gtaagttatt gtcacatatt gaaacgacat catcaacact tgagaaacag
    24361 ttacatgcgc tgaagcgtca tcgaaatgtt ggtgatgtaa gaggacgagg cttaatgttt
    24421 ggagttgaat tagttacaga taaagatagt aaaacgccgt tagaaattga aaaagttgaa
    24481 cgtattgtac gtaaatgtaa agaaaacggg ctaatgatta gaaatttaga aaatgtcatt
    24541 acgtttgtgc cagtgttaag tatgtcaaat aaagaagtga aaacgatggt acgtattttt
    24601 aaaaaggcag tacataacat tttagatagg aagtgttaat atgaatttgg ctaaacgcat
    24661 attacaaggg gaacaattaa caaaagagac tgtattgaaa atttatgagg atactaatat
    24721 tgatacctta gatttattaa atgaggcgta cattttaaga aaacattatt ttggtaaaaa
    24781 agtaaaatta aacatgattt taaatgctaa aagtggtata tgtcctgaga attgtgggta
    24841 ctgtggacaa tcacgagata ttaaacaaaa acagcgatat gctttaattc cagaggaaca
    24901 aattatcgat ggagcaaagg tggcacatga taatcatatt ggaacatatt gtattgttat
    24961 gagtggtaga ggaccgagcg ataaagaagt tgatcatatt agtaatactg taagaacgat
    25021 taaatctcaa cacccgcaac taaaaatctg tgcatgttta ggattaacga atgacgaaca
    25081 agctaagaaa cttaagtcag ctggtgtaga cagatataac cacaatatta atacaagtga
    25141 aaattaccat gataacgtcg tgacaacgca tagttataaa gatagaacag atacgataga
    25201 actaatgaaa gcgaataata tatcaccatg ttctggcgtg atttgtggta tgggagaatc
    25261 taatcaagat attgttgata tggcatttgc tttaaaagaa atggatgccg acagtattcc
    25321 gattaatttt ttgcatccaa tcaaaggcac aaagtttgga agcatggatg atttaacacc
    25381 aatgaaatgt ttaagaatcg tagcattatt ccgattaatc aatcctacga aagaaattcg
    25441 tattgctgga ggaagagagg tcaacttacg ttcgttacag ccattagcat taaaagcggc
    25501 gaattcaata tttgtcggtg attatctaat tactggtggg caaccgaacc aattagacta
    25561 cgatatgatt aatgatttgg gctttgaaat tgattatgac acttgtgaaa ataaggaaaa
    25621 caagaatgat gtttcaagag caaattgatt tattacaaca gaaagggtta tatcggtcgc
    25681 ttaaatcggt agggtatata gatagacagt atattgaagt agaaaataag agatgtacga
    25741 actatacatc taatgattat ttaggattag gtcaaatagc gtttgataag gatgatttcg
    25801 aaagatttat gcggaagtat agttatcact tatcaagttc aagattaatt agtggaagtt
    25861 cgacagctta tgaagaaatt gaaacaatgt tagcaggttg gctcggatat agtgcatgta
    25921 ctatcttaaa tagtggttat gatgctaatt tggcgttgtt taatattttc aaaaatacaa
    25981 attgtgtcgt gttttcagat caagaaaatc atgcgagtat tattgacggt attaagttaa
    26041 gtggtttaga aaaagtgata tataagcatt tagatattgc tgatttagaa aaaaggttag
    26101 agaaataccc taatcaaaat ataccaaaaa taatcatatc tgatagtgta ttttcaacga
    26161 atggcgacgt tgtggatatt ggtcaattag tctcattaaa gcataaatat aacgcaacac
    26221 tgatactcga tgtttcacat agttttggaa tagagaatta ctcgaattat caaggtgtag
    26281 atatactcac ttctagttta tctaaagcat gtggtgcata cgggggtgtg attttaagtt
    26341 caaatgatgt gaaggatatg ttaattaatc acggtagacc actcatctac tcaagtagtt
    26401 tgccaattta taatttgtat tttataaaaa gaaacattga aaagttaata aatgctgatg
    26461 atagacgcac taaattaaat agtttgagta aatattttaa ccaaaagtta aaagcactca
    26521 atgttaatta taatagttca aactcgccga ttaagtttat tgagtttgat gacatagaag
    26581 cggctgagaa cattcatcaa acattattaa agcatcatgt gtttacaagt tatttaaggt
    26641 atccaactgt gactaagcca atgttaagaa tatcattgtc gtattttcat actgaacaag
    26701 atatagatag gttgtttgaa attttgcatc aagaagattg aggtgatcgt catgtatagt
    26761 ataaaaatgc gttctagcaa tcaagatgtt catattagtg gtgctgaaac gatttgtgag
    26821 tttgacaaaa tagaacagac ggtacaaagg ttttataata aaggtttttt tcatgaaaat
    26881 gggcaaccgg attttctcaa tataaaaata caaaaaatta tggaaccgat tcaacaaata
    26941 aaagcattac aaattattga ggatgataaa gcaaatctcc aacatttaac acaagaatgt
    27001 ggtgtcactg aacaagcgct aaatcaaggg atgacatata ttaaaaatga aacggtttat
    27061 acaggagcta ttattctatc tgctatatct ggtaaacgtt tagattcatt tggacaaagg
    27121 gggattaggg caacacattt ttcgtttgaa gatataaata ataaaggtga tcttaatgaa
    27181 agagtgactg atgccttagc aattgctagt tgtatcaatg cgcatccgta tgtcaaagga
    27241 gaactttgcg tgtccgatga cttaacgtat acgacaggtt attttgccgc tgctaaaatt
    27301 ggttaccatc gattatttga tattaaacca gttaatacga gatatggagg cagaataata
    27361 tttgtggacg attgtattga tttaaatcat tacatatcat ttttagaaag cacaccgaag
    27421 caagttgttt atgaaacggt ataggggttt tagtatgaca tcaaaagata ttactcaaat
    27481 tagtgtcatt gctgcgattt taaccatttt ggcagttttg aaaataccgt ccattatacc
    27541 aggattagat tttcaattat ctgcaccggc agcattattg atattagctt tctttggaat
    27601 taaaaagtac tttttaggtg ggcttttatc aagcgtatta ttactgatat ttggcgtatt
    27661 taatccaatt aatgtgatta tttctattat atttagagtt atagctattg cagttgttta
    27721 tttattgaaa ataaatgtac tatcattagt tttatcaagt gtattaggca gtttggtatc
    27781 taggctacta ttatctatta ttttaaattt acctgtgtgg gtagtgttgt taaacgcgat
    27841 tccaggcgta atattcactt taattgtagc tattccttta tatctcacat tgagaaaaag
    27901 aatggcagtc ttactaagat aataaatcaa aacacggtcg tcacaattac tgttggcgac
    27961 cgtgttttac tagctattta ttgttttcag tttcttttgt atctaacaat ttcactttgt
    28021 gattttccca atcaatttca tatgttgatt taaatgttct agttttaaag tttttataat
    28081 ttgcgcctgc ccagtagaag ccattccaac gaatttggta taaatccatt tcacgttgat
    28141 aagttactgt aattttagat tttttagcgc catcttgtct gtgtgatagt acgcttaaaa
    28201 attctggatt gaagttactt ctagataata atggcatttg gtgttgcgct atgaagtttt
    28261 ggccagcgta tgcactgctt tgtctgccag ctaagaagag ttcattacca tatgttgggt
    28321 ggaagctatc tcttccataa ggtccccaac cattattcat aattttatgt gcttcaactc
    28381 cccagccaac atttttataa tttgtgttgc gacttaatgt tgttctgtaa ctttcttgtt
    28441 tataattaat tgtttcagaa aaagctgtat ttccattaag tccaccagat aaaccattag
    28501 agatactaat gtcaccacca aatgtatagc ctaaagtatt ttgaacttga aactcttcat
    28561 tttgattttt tggtgcataa tcaacgacgt ttactgaatc attagattgt gagcttatag
    28621 atacattgta tttagctccc caatataatt ttgaaaagtc atagtcatta ggattaggtt
    28681 tcacaaagcc tgagttaata ttcccagtag ctttaagtac taaagtatct ttatcataac
    28741 ttttatcttt gatgaaatta aatgttaaaa tctgtgaaat tttaaactta tcagaatctg
    28801 ctgtggctgt tgttttgtat aaagtaactt tgtcatcgac tttttttacg ctgactggtg
    28861 ttattttacc ttcagcatta gcagtaccag aaagtaataa taatgccata gatgtagcaa
    28921 cggatgattt gactaattta ttcattttca tatcaattct gtcctttcac cttgatttca
    28981 tgagtcttcc aattgacctc gtatttaaca gtatagtttc tatttacgaa tgcattatgg
    29041 actctatgtc cgtctaaata actgttgcca taatgcgttg atcttttaat ggcatgagtg
    29101 acatccatgt ttcttccgta agtaatttca aattcgcttg tatcgcttga acctttttca
    29161 tgagatactg tggcgataaa tgaagggtta aatccacttt gtaaaagagg aggtaactca
    29221 ctgtctggaa cgaaataatc tctaggatct ttactatgag gtttgtagcc tacaaataaa
    29281 tcgctatcaa atgctgattt ttgacctgat tcagtggcga atgaattcgc tttgacgccc
    29341 cataaaacac tttttgagtt ttgttgttct acttcactta cataattttg ttgtgtatag
    29401 ctaatcgatt tagaatagtt aaatgatcca ttaccaccga gtgatggggc tgattggaaa
    29461 ttaccaccga tattgtatcc taatgtctga ctcacgtttg tagattcaat tttattttta
    29521 ggtaaataat taattaaaga aacatattta tcatttgttt ttaaaccaat attatattgg
    29581 aatggccatc gcatagcttt aacatgatta gtttttttat agttgtaata tgttgttcta
    29641 gagctaatga atccttgcat ctttaatatc agagcatctt tgttatattt tttatccttc
    29701 acaaaatcaa attgaatatt ttgagtcacg ccccatttat tacttgtttt atcttctgtc
    29761 cttttgataa tttctatatc gcttccttta ccgatgtctt cagtatcgtt ggcagcttta
    29821 gcattttcta ataacggatt ggcaagaggg gcaagtaagc tcacagataa agttgtagct
    29881 aatattttat ttttaagcat aagtttcact ttctttctat aattttattt aacttaat
//
LOCUS       DAHHSC010000013        29809 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.013, whole genome shotgun sequence.
ACCESSION   DAHHSC010000013 DAHHSC010000000
VERSION     DAHHSC010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 29809)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29809)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29809
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.013"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            complement(439..544)
                     /locus_tag="NBI57_000451"
                     /pseudo
     CDS             complement(439..544)
                     /locus_tag="NBI57_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499028.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(749..1345)
                     /gene="recR"
                     /locus_tag="NBI57_000452"
     CDS             complement(749..1345)
                     /gene="recR"
                     /locus_tag="NBI57_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009383386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HCD1821433.1"
                     /translation="MHYPEPISKLIDSFMKLPGIGPKTAQRLAFHTLDMKEDDVVQFA
                     KALVDVKRELTYCSVCGHITENDPCYICEDKQRDRSVICVVEDDKDVIAMEKMREYKG
                     LYHVLHGSISPMDGIGPEDINIPSLIERLKSDEVSELILAMNPNLEGESTAMYISRLV
                     KPIGIKVTRLAQGLSVGGDLEYADEVTLSKAIAGRTEM"
     gene            complement(1352..1669)
                     /locus_tag="NBI57_000453"
     CDS             complement(1352..1669)
                     /locus_tag="NBI57_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002479279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HCD1821434.1"
                     /translation="MRGGGNMQQMMKQMQKMQKKMAQEQEKLKEERIVGTAGGGMVAV
                     TVTGHKEVVDVEIKEEAVDPDDIEMLQDLVLAATNEAMNKADELTQERLGKHTQGLNI
                     PGM"
     gene            complement(1759..3456)
                     /gene="dnaX"
                     /locus_tag="NBI57_000454"
     CDS             complement(1759..3456)
                     /gene="dnaX"
                     /locus_tag="NBI57_000454"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HCD1821435.1"
                     /translation="MNYQALYRMYRPQSFEDVVGQEHVTKTLRNAISKEKQSHAYIFS
                     GPRGTGKTSIAKVFAKAINCLNSTDGEPCNECHICKGITQGTNSDVIEIDAASNNGVD
                     EIRNIRDKVKYAPSESKYKVYIIDEVHMLTTGAFNALLKTLEEPPAHAIFILATTEPH
                     KIPPTIISRAQRFDFKAISLDQIVERLKFVADAQQIECEDEALAFIAKASEGGMRDAL
                     SIMDQAIAFGDGTLTLQDALNVTGSVHDEALDHLFDDIVQGDVQASFKKYHQFITEGK
                     EVNRLINDMIYFVRDTIMNKTSEKDTEYRALMNLELDMLYQMIDLINDTLVSIRFSVN
                     QNVHFEVLLVKLAEQIKGQPQVIANVAEPAQIASSPNTDVLLQRMEQLEQELKTLKAQ
                     GVSVAPAQKSSKKPARGIQKSKNAFSMQQIAKVLDKANKADIKLLKDHWQEVIDHAKN
                     NDKKSLVSLLQNSEPVAASEDHVLVKFEEEIHCEIVNKDDEKRSSIESVVCNIVNKNV
                     KVVGVPSDQWQRVRTEYLQNRKNEGDDMPKQQAQQTDIAQKAKDLFGEETVHVIDEE"
     gene            complement(3525..4049)
                     /locus_tag="NBI57_000455"
     CDS             complement(3525..4049)
                     /locus_tag="NBI57_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCD1821436.1"
                     /translation="MQIYLSTLTELDYDKSLNSIEESFDDNPETSWQARAKVKHLRKS
                     PCYNFELEVIAKNENNDVVGHVLLIEVEINSDDKTYYGLAIASLSVHPELRGQKLGRG
                     LVQAVEERAKAQEYSTVVVDHCFDYFEKLGYQNAAEHDIKLESGDAPLLVKYLWDNLT
                     DAPHGIVKFPEHFY"
     gene            complement(4187..4288)
                     /locus_tag="NBI57_000456"
     CDS             complement(4187..4288)
                     /locus_tag="NBI57_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1821437.1"
                     /translation="MKTIQRIIRGTCLWEVAFLYVKFDSSELDVQFE"
     gene            complement(4361..4630)
                     /gene="ffs"
                     /locus_tag="NBI57_000457"
     ncRNA           complement(4361..4630)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="NBI57_000457"
                     /product="signal recognition particle sRNA large type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01854"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF01854"
     gene            complement(4692..5420)
                     /gene="treR"
                     /locus_tag="NBI57_000458"
     CDS             complement(4692..5420)
                     /gene="treR"
                     /locus_tag="NBI57_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HCD1821438.1"
                     /translation="MAKQKKFMKIYEALKEDILNGQIQYGEQIPSEHDLVQLYQSSRE
                     TVRKALDLLALDGMIQKIHGKGSLVIYQEVTEFPFSELVSFKEMQEEMGVAYLTEVVV
                     NEVVEAHEVPEVQHALNINSSESLIHIVRTRRLNQHVKIVDEDYFLKSIVSDIGNDVA
                     SDSIYDYLEKVLNLNISYSSKSITFEPFDEQAYQLFGDVSVAYSATVRSIVYLENTMP
                     FQYNISKHLANEFKFNDFSRRRIK"
     gene            complement(5445..7085)
                     /gene="treC"
                     /locus_tag="NBI57_000459"
     CDS             complement(5445..7085)
                     /gene="treC"
                     /locus_tag="NBI57_000459"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HCD1821439.1"
                     /translation="MSKEIDWRKSVVYQIYPKSFNDTTGNGIGDINGIIEKLDYIKLL
                     GVDYIWLTPVYESPMNDNGYDISNYLEINEAFGTMDDFEKLIKVAHQKDLKVMLDIVI
                     NHTSTEHEWFKEARKSKDNPYRDYYFFRSSEDGPPTNWHSKFGGNAWKYDSETDEYYL
                     HLFDVSQADLNWDNPEVRQSLYRIVNHWIDFGVDGFRFDVINLISKGEFKDSDKIGKE
                     FYTDGPRVHEFLHELNRQTFGNTDMMTVGEMSSTTIENCIKYTQPERQELNSVFNFHH
                     LKVDYVDGEKWTNAKLDFHKLKEILMQWQRGIYDGGGWNAIFWCNHDQPRVVSRFGDD
                     TSEEMRIQSAKMLAIALHMLQGTPYIYQGEEIGMTDPHFTSIAQYRDVESINAYHQLL
                     SEGHAEADVLAILGQKSRDNSRTPMQWSDDVNAGFTAGKPWIDISENYHQVNVRQALQ
                     NKESIFYTYQKLIQLRHTHDIITYGDIVPRFMDHDHLFVYERHYKNQQWLVIANFSAS
                     AVDLPEGLAREGCVVIQTGTVENNTISGFGAIVIETNA"
     gene            complement(7149..8576)
                     /gene="treP"
                     /locus_tag="NBI57_000460"
     CDS             complement(7149..8576)
                     /gene="treP"
                     /locus_tag="NBI57_000460"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HCD1821440.1"
                     /translation="MAVKREDVKAIVSAIGGKENLEAATHCVTRLRLVLKDESKVDKD
                     ALSNNALVKGQFKADHQYQIVIGPGTVDEVYKQFIDETGAQEASKDEAKQAAAKKGNP
                     VQRLIKLLGDIFIPILPAIVTAGLLMGINNLLTMKGLFGPKALIEMYPQIADISNIIN
                     VIASTAFIFLPALIGWSSMRVFGGSPILGIVLGLILMHPQLVSQYDLAKGNIPTWNLF
                     GLEIKQLNYQGQVLPVLIAAYVLAKIEKGLNKVVHDSIKMLVVGPVALLVTGFLAFII
                     IGPVALLIGTGITSGVTFIFQHAGWLGGAIYGLLYAPLVITGLHHMFLAVDFQLMGSS
                     LGGTYLWPIVAISNICQGSAAFGAWFVYKRRKMVKEEGLALTSCISGMLGVTEPAMFG
                     VNLPLKYPFIAAISTSCVLGAIVGMNNVLGKVGVGGVPAFISIQKEFWPVYLIVTAIA
                     IVVPCILTIVMSHFSKQKAKEIVED"
     gene            complement(9045..9137)
                     /locus_tag="NBI57_000461"
     tRNA            complement(9045..9137)
                     /locus_tag="NBI57_000461"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9099..9101),aa:Ser,seq:tga)
     gene            complement(9406..10869)
                     /locus_tag="NBI57_000462"
     CDS             complement(9406..10869)
                     /locus_tag="NBI57_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit beta"
                     /protein_id="HCD1821441.1"
                     /translation="MGEFKGFMKYDKQYLGELSLVDRLKHHKAYQQRFTKEDASIQGA
                     RCMDCGTPFCQTGQQYGRETIGCPIGNYIPEWNDLVYHQDFKTAYERLSETNNFPDFT
                     GRVCPAPCESACVMKINRESIAIKGIERTIIDEAFENGWVAPKVPSRRRDEKVAIVGS
                     GPAGLTAAEELNLLGYQVTIYERARESGGLLMYGIPNMKLDKDVVRRRIKLMEEAGIT
                     FINGVEVGVDIDKATLESEYDAIILCTGAQKGRDLPLEGRMGDGIHFAMDYLTEQTQL
                     LNGEIDDITITAKDKNVIIIGAGDTGADCVATALRENCKSIVQFNKYTKLPEAITFTE
                     NASWPLAMPVFKMDYAHQEYEAKFGKEPRAYGVQTMRYDVDDKGHIRGLYTQILEQGE
                     NGMVMKEGPERFWPADLVLLSIGFEGTEPTVPNAFNIKTDRNRIVADDTNYQTNNEKV
                     FAAGDARRGQSLVVWAIKEGRGVAKAVDQYLASKVCV"
     gene            complement(10887..15386)
                     /gene="gltB"
                     /locus_tag="NBI57_000463"
     CDS             complement(10887..15386)
                     /gene="gltB"
                     /locus_tag="NBI57_000463"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HCD1821442.1"
                     /translation="MHNEKLIKGLYDYREEHDACGIGFYANMDNKRSHDIIDKSLEML
                     RRLDHRGGVGADGITGDGAGIMTEIPFAFFKQHVTDFDIPGEGEYAVGLFFSKERILG
                     SEHEAVFKKYFEGEGLSILGYRNVPVNKDAIAKHVADTMPVIQQVFIDIRGIEDVEKR
                     LFLARKQLEFYSTQCDLELYFTSLSRKTIVYKGWLRSDQIKKLYTDLSDDLYQSKLGL
                     VHSRFSTNTFPSWKRAHPNRMLMHNGEINTIKGNVNWMRARQHKLIETLFGEDQHKVF
                     QIVDEDGSDSAIVDNALEFLSLAMEPEKAAMLLIPEPWLYNEANDANVRAFYEFYSYL
                     MEPWDGPTMISFCNGDKLGALTDRNGLRPGRYTITKDNFIVFSSEVGVVDVPESNVAF
                     KGQLNPGKLLLVDFKQNKVIENNDLKGAIAGELPYKAWIDNHKDDFDFENIQYQDSQW
                     KDETLFKLQRQFAYTKEEIHKYIQELVEGKKDPIGAMGYDAPIAVLNERPESLFNYFK
                     QLFAQVTNPPIDAYREKIVTSELSYLGGEGNLLAPDETVLDRIQLKRPVLNESHLAAI
                     DQEHFKLTYLSTVYEGDLEDALEALGREAVNAVKQGAQILVLDDSGLVDSNGFAMPML
                     LAISHVHQLLIKADLRMSTSLVAKSGETREVHHVACLLAYGANAIVPYLAQRTVEQLT
                     LTEGLQGTVVDNVKTYTDVLSEGVIKVMAKMGISTVQSYQGAQIFEAIGLSHDVIDRY
                     FTGTQSKLSGISIDQIDAENKARQQSDDNYLASGSTFQWRQQGQHHAFNPESIFLLQH
                     ACKENDYAQFKAYSEAVNKNRTDHIRHLLEFKACTPIDIDQVEPVSDIVKRFNTGAMS
                     YGSISAEAHETLAQAMNQLGGKSNSGEGGEDAKRYEVQVDGSNKVSAIKQVASGRFGV
                     TSDYLQHAKEIQIKVAQGAKPGEGGQLPGTKVYPWIAKTRGSTPGIGLISPPPHHDIY
                     SIEDLAQLIHDLKNANKDADIAVKLVSKTGVGTIASGVAKAFADKIVISGYDGGTGAS
                     PKTSIQHAGVPWEIGLAETHQTLKLNDLRSRVKLETDGKLLTGKDVAYACALGAEEFG
                     FATAPLVVLGCIMMRVCHKDTCPVGVATQNKDLRALYRGKAHHVVNFMHFIAQELREI
                     LASLGLKRVEDLVGRTDLLQRSSTLKANSKAASIDVEKLLCPFDGPNTKEIQQNHNLE
                     HGFDLTNLYEVTKPYIAEGRRYTGSFTVNNEQRDVGVITGSEISKQYGEAGLPENTIN
                     VYTNGHAGQSLAAYAPKGLMIHHTGDANDYVGKGLSGGTVIVKAPFEERQNEIIAGNV
                     SFYGATGGKAFINGSAGERFCIRNSGVDVVVEGIGDHGLEYMTGGHVINLGDVGKNFG
                     QGMSGGIAYVIPSDVEAFVENNQLDTLSFTKIKHQEEKAFIKQMLEEHVSHTNSTRAI
                     HVLKHFDRIEDVVVKVIPKDYQLMMQKIHLHKSLHDNEDEAMLAAFYDDSKTIDAKHK
                     PAVVY"
     gene            15567..16451
                     /locus_tag="NBI57_000464"
     CDS             15567..16451
                     /locus_tag="NBI57_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCD1821443.1"
                     /translation="MEIKQLRYFIEVAKREHISETALELNIAQSAISRQITLLEQELN
                     VSLFKKQGRNITLTSEGKLLFNEALRIIEHLDSTIEQFQSHGLTKNKSIYIGYDESDV
                     SHMLLPLIQTFHLQNDTHVIPSLLDHDTIINSVLNGNIDIGFTELTPEIRKHKQLHML
                     PLFEEHYHLYAPSDDPITMATHPPLIQFEHSHIYCLAPFAETVKKQLRKITKSDVYTI
                     SSQPLAQYLLRQKEGYIISSQNIHLPESKSWIDIKLDHTELKRTICAITKEPYTKSDI
                     GILLTLIQQLMTKTSTFH"
     gene            16566..17684
                     /locus_tag="NBI57_000465"
     CDS             16566..17684
                     /locus_tag="NBI57_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HCD1821444.1"
                     /translation="MFLKYRNLFTKPFNIILGLFCIAFIGAILFTFNNENFYNKPIGQ
                     IIDVKHVSSTPTKDAQNNRDIKYKNQLKVKILNGQFAGETTTINHQYVKSQADSEAFR
                     THEKVLLHISNKPSDAYIIEKKRDTLTVIITGLFLLTVLLVGRKVGLQSILSLILNSI
                     AILIAIYIHIQHSNINLFLLMTIAMICSTILTLLLVTGWHMRTLITIASTIIGTFLSI
                     GLTELIIYMTDGKGIKYETMNFLSLPPKDIFLASVLIGSLGAIMDVAITIASGMHEIL
                     QRTPHISMRRWALAGRNIGQDIMGTMTNILLFSYLSGALPMFLIFLKNANTVTYTISM
                     NWSLEIARALTGGIGIVLTIPITILFMEIFETLRRAKQ"
     gene            17681..18463
                     /locus_tag="NBI57_000466"
     CDS             17681..18463
                     /locus_tag="NBI57_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HCD1821445.1"
                     /translation="MNAVFILALLLLFLMIIFGGKKGFISYLTLFLNFAILIISIVLI
                     IFGVPIYVVTFFFCIIIGACNLFVLNSYNVKTQAAFISTIVTTILLITLIYLSVYFGH
                     LQGFSTEQQDETYVYSMNIGIDMVQFMVFTIILAVIAAVIDLTITISSPIYELHQVNP
                     TLTQYELFQSGMRVGREILATSANTIYLAFFGGQLALFIWFFKLKYSFGHIINSKIFA
                     QEFISIILGGIAVAISIPITAWITAFLIKRPSRQRKNDESIN"
     gene            complement(18632..19117)
                     /locus_tag="NBI57_000467"
     CDS             complement(18632..19117)
                     /locus_tag="NBI57_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCD1821446.1"
                     /translation="MTNNDTIMLRHYVPQDYSMLEAFQLSESDLKFVKTPEENITAAM
                     SDNERYPIVVMDGRQCVAFFTLHRGKGVAPFSDNQDAVFFRSFSVDQRYRNRGIGKVV
                     MEKLASFITSTFQDINEIVLTVNTDNPHAMALYRQQGYQYMGDSMFIGRPVHIMALTI
                     K"
     gene            complement(19107..19502)
                     /locus_tag="NBI57_000468"
     CDS             complement(19107..19502)
                     /locus_tag="NBI57_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HCD1821447.1"
                     /translation="MIKCVCLVEETADKILLVQVRNREKYYFPGGKIEEGESQVHALL
                     REVKEELNLTLTMDEIEYIGTIVGPAYPQQDMLTELNGFRALTKIDWENVTINNEITD
                     IRWIDKDNDALIAPAVKVWIETYGGKHDK"
     gene            19651..19920
                     /locus_tag="NBI57_000469"
     CDS             19651..19920
                     /locus_tag="NBI57_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1821448.1"
                     /translation="MAKRSKSQRLSSLLNVAGFIVDGYNGYKYHAKNKKLVYLSLGLS
                     TVGTVLDFYISIKSPRKFKKAVAVVTLITNGARLFTSIRKVKHEY"
     gene            complement(20105..21094)
                     /gene="aaa"
                     /locus_tag="NBI57_000470"
     CDS             complement(20105..21094)
                     /gene="aaa"
                     /locus_tag="NBI57_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autolysin/adhesin Aaa"
                     /protein_id="HCD1821449.1"
                     /translation="MAAIIGTSAISAVAATQANAATTHTVKPGESVWAISNKYGISIA
                     KLKSLNNLTSNLIFPNQVLKVSGSSNSTSNSSRPSTNSGGGSYYTVQAGDSLSLIASK
                     YGTTYQNIMRLNGLNNFFIYPGQKLKVSGTASSSNAASNSSRPSTNSGGGSYYTVQAG
                     DSLSLIASKYGTTYQKIMSLNGLNNFFIYPGQKLKVTGNASTNSGSATTTNRGYNTPV
                     FSHQNLYTWGQCTYHVFNRRAEIGKGISTYWWNANNWDNAAAADGYTIDNRPTVGSIA
                     QTDVGYYGHVMFVERVNNDGSILVSEMNYSAAPGILTYRTVPAYQVNNYRYIH"
     gene            complement(21440..22282)
                     /gene="gmpC"
                     /locus_tag="NBI57_000471"
     CDS             complement(21440..22282)
                     /gene="gmpC"
                     /locus_tag="NBI57_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter
                     glycylmethionine-binding lipoprotein"
                     /protein_id="HCD1821450.1"
                     /translation="MKRLIGLVIVALVLLAACGSNNDKKVTIGVASNDTKAWEKVKEL
                     AKKDDIDVEIKHFSDYNLPNKALNDGDIDMNAFQHFAFLDQYKKAHKGTKISALSTTV
                     LAPLGIYSDKIKDVKKVKDGAKVVIPNDVSNQARALKLLEAAGLIKLKKDFGLAGTVK
                     DITSNPKHLKITAVDAQQTARALSDVDIAVINNGVATKAGKDPKNDPIFLEKSNSDAV
                     KPYINIVAVNDKDLDNKTYAKIVELYHSKEAQKALQEDVKDGEKPVNLSKDEIKAIET
                     SLAK"
     gene            complement(22319..22978)
                     /locus_tag="NBI57_000472"
     CDS             complement(22319..22978)
                     /locus_tag="NBI57_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCD1821451.1"
                     /translation="MFGSDLDSAQLLQALYETLYMVSIALFLGAVIGIPLGVLLVITR
                     KQGIWPNIVIHQVLNPLINILRSLPFIILLIAIVPFTKLVVGTSIGTTAAIVPLTVYV
                     APYIARLVENSLLEVDEGIIEAAKAMGASPLQIIRYFLIPEALGSLVLAITTAIIGLI
                     GSTAMAGAVGGGGIGDLALVYGYQRFDTTVIIITVIVLVIIVQVIQTLGNVLARFIRR
                     H"
     gene            complement(22982..24007)
                     /locus_tag="NBI57_000473"
                     /pseudo
     CDS             complement(22982..24007)
                     /locus_tag="NBI57_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499008.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
     gene            complement(24302..25444)
                     /locus_tag="NBI57_000474"
     CDS             complement(24302..25444)
                     /locus_tag="NBI57_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional cystathionine
                     gamma-lyase/homocysteine desulfhydrase"
                     /protein_id="HCD1821452.1"
                     /translation="MNKKTKLIHGGHTTDDYTGAVTTPIYQTSTYLQDDIGDLRQGYE
                     YSRTANPTRSSVESVIAALENGKHGFAFSSGVAAISAVVMLLDKGDHIILNSDVYGGT
                     YRALTKVFTRFGIEVDFVDTTHTDSIVQAIRPTTKMLFIETPSNPLLRVTDIKKSAEI
                     AKEHGLISVVDNTFMTPYYQNPLDLGIDIVLHSATKYLGGHSDVVAGLVATSDDKLAE
                     RLAFISNSTGGILGPQDSYLLVRGIKTLGLRMEQINRSVIEIIKMLQAHPAVQQVFHP
                     SIESHLNHDVHMAQADGHTGVIAFEVKNTESAKQLIKATSYYTLAESLGAVESLISVP
                     ALMTHASIPADIRAKEGITDGLVRISVGIEDTEDLVDDLKQALDTL"
     gene            complement(25437..26342)
                     /locus_tag="NBI57_000475"
     CDS             complement(25437..26342)
                     /locus_tag="NBI57_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000634108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HCD1821453.1"
                     /translation="MITYDLIGNTPLVLLEHYSDDKVKIYAKLEQWNPGGSVKDRLGK
                     YLVEKAIQEGRVRAGQTIVEATAGNTGIGLAIAANRHHLKCKIFAPYGFSEEKINIMI
                     ALGADVSRTSQSEGMHGAQLAARSYAEKYGAVYMNQFESEHNPDTYFHTLGPELTSAL
                     QQIDYFVAGIGSGGTFTGTARYLKQHHVQCYAVEPEGSVLNGGPAHAHDTEGIGSEKW
                     PIFLERRLVDGIFTIKDQDAFRNVKSLAINEGLLVGSSSGAALQGALNLKAQLSEGTI
                     VVVFPDGSDRYMSKQIFNYEENDYE"
     gene            complement(26559..27896)
                     /locus_tag="NBI57_000476"
     CDS             complement(26559..27896)
                     /locus_tag="NBI57_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HCD1821454.1"
                     /translation="MKRQQSQWKSSTGFILASAGSAIGLGAMWKFPYMAGIYGGGAFL
                     AMFLIFTIFVGLPLLIMEFTVGKMGRTYTTQIYSKLTGKKWLNIIGWNGNLAVFILFG
                     FYSVIGGWIVIYIGQVLWQLVIFQRINHLQEMNFEAVISNPWLTVLGQGIFIFATMII
                     VMLGVEKGLEKASKVMMPLLFVFLIIIVIKSLTLDGALEGVKFILQPRVSEITADGIL
                     FALGQSFFTLSLGTTGMITYASYASKDMTIKSSAISIVVMNIFVSVLAGLAIFPALHS
                     FGYEPQEGPGLLFKVLPMVFSQMHLGTLFYLGFLVLFLFAALTSSISLLELNVSNFTK
                     NDNTKRKKVAVIGSILVFIISIPATLSFGILKDVRFGAGTIFDNMDFIVSNVLMPLGA
                     LGTTLVIGQLLDKKLLQQYFGKDRFRLFNGWYYLIKYAMPVVIILVFIVQLFS"
     gene            complement(28268..29002)
                     /locus_tag="NBI57_000477"
     CDS             complement(28268..29002)
                     /locus_tag="NBI57_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylesterase"
                     /protein_id="HCD1821455.1"
                     /translation="MRIKTPSPSYLKGTNGHAILLLHSFTGTNRDVKHLAAELNDQGF
                     SCYAPNYPGHGLLLKDFMTYNVDDWWEEVEKAYQFLVNEGYESISATGVSLGGLMTLK
                     LAQHYPLKRIAVMSAPKEKSDDGLIEHLVYYSQRMSNILNLDQQASSAQLAAIDDYEG
                     EITKFQHFIDDIMTNLNVIKIPANILFGGKDEPSYETSAHFIYEHLGSVDKELNGLKD
                     SHHLMTHGEGRDILEENVIRFFNALT"
     gene            complement(29039..29713)
                     /locus_tag="NBI57_000478"
     CDS             complement(29039..29713)
                     /locus_tag="NBI57_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HCD1821456.1"
                     /translation="MIDKKLTSPKMTVPLFLIALIVFIGMFYGVVTNQEWLKNIDMGS
                     LTWFTDYFGEPQRQYVNNLFNYYMTFSAEIGDVKGVVLISIIVTIILFIKQRHLAVWF
                     VTYLVSGVIMNKLIKDTVLRPRPYNHLAVDTGFSFPSGHSNASTLLYFALMIIIISVA
                     AKTITKVLSALVMGILWLSILFCRLYFHAHYFSDVIGGTSLAIIWVALFLMVYPYFIN
                     HRRQRV"
BASE COUNT         9392 a         6246 c         4251 g         9920 t
ORIGIN      
        1 cttaccgaca agaattaatt atacatttta ttaacattta agtcaacaac tttttaaaat
       61 taattttcgt tagtaattca agttatttct taagttgagt tgttttgttt gcctcaacaa
      121 ataaatacta tacaggcttt caaactgctt aacaacagta aaaattacac ctttgtcatc
      181 tgtactgaac gcccttatcc taaaaatcga acttaaaaaa tccccactta taaatagtac
      241 gttttttaga acaagaaaca gatttttctc taaccatatc aatcaaaaaa accgcaaaac
      301 agaacgttat ttttttacca gaaattaaaa ataaatttac aagttttaaa taatctctca
      361 aaacaccgct atacaggctg atttaataag ctttagacca aagaagtaca actactaagc
      421 cagcatctat catttcaatt acaaaagaat ttcatttgca tagtcattaa ccatcaaccg
      481 catttatcaa attaatagca ccaacacacc ataaccgtaa atctaattcg ataatccaat
      541 tcacatcatt actattagaa gtaacaaatc acaaccaacg gcttgcttcc taaagattac
      601 ccatccctca gtaaaactag gtaaaccata ccaccgccac accgcaaata aagtagtaca
      661 tcacaccaca ccataaaaaa agccacaatg attacactgt gacttttctt actatagtat
      721 taaattcaaa aatgtttaat agaagacatt acatttctgt tctacctgcg attgctttag
      781 ataatgttac ttcgtcagca tactctaaat cgccacctac cgataaccct tgtgctaatc
      841 tcgtcacttt gatacctata ggcttaacta atctagaaat atacatggct gtagattccc
      901 cctctaagtt cgggttcata gctaagatta attcgctaac ttcatcgctt ttcaagcgtt
      961 caatcaatga aggaatatta atatcttctg gtccaatgcc atccataggt gaaatagacc
     1021 catgtaaaac gtgatataaa cctttgtatt ctctcatttt ttccatagct atgacatctt
     1081 tgtcatcttc cacaacacaa ataactgaac gatctctttg tttatcttca caaatataac
     1141 atggatcatt ttcagtaatg tgaccacata cgctacaata tgttaattct ctcttaacat
     1201 ctactaatgc tttggcaaac tgaacaacat cgtcttcttt catatctaag gtatgaaaag
     1261 ccagacgttg ggctgtcttt ggaccaatgc ctggcaattt cataaagcta tcaataagct
     1321 ttgatatagg ttctggataa tgcatctatg atcacattcc agggatgttt aagccttgag
     1381 tatgtttacc taaacgttct tgagtaagct catcagcttt attcatcgct tcattagtag
     1441 ctgctaacac taagtcttgt agcatttcaa tatcgtctgg gtctacagct tcttctttga
     1501 tttcaacgtc gacaacttct ttatgaccag ttacagtaac tgcaaccatg ccaccgccag
     1561 ctgttcctac aatacgctct tctttaagtt tttcttgttc ttgagccatt ttcttttgca
     1621 ttttttgcat ttgtttcatc atttgttgca tgtttccgcc accgcgcata ttccatttcc
     1681 tccttgaaaa tcaataaata tttatcaata aaatgatgtt tctttcatta tacatacgat
     1741 tatatcgctt gtcatgtatc actcttcatc tatcacatgt acagtttctt caccgaaaag
     1801 atcttttgct ttttgagcaa tatctgtttg ttgtgcttgt tgctttggca tatcatcgcc
     1861 ttcgttttta cgattttgta aatactccgt tcgaactctt tgccattgat ctgatggtac
     1921 accaacaact ttaacgtttt tattaacgat attacataca acactttcta tactactacg
     1981 tttctcgtcg tctttattga cgatttcaca atggatctct tcctcaaatt tcacaagtac
     2041 gtgatcttca cttgccgcca caggttccga attttgcaat aaactaacga gtgatttttt
     2101 atcattgttt ttggcatgat caatcacttc ttgccaatga tctttcaaca atttgatatc
     2161 tgccttattc gctttatcta gcacttttgc aatttgttgc attgaaaatg catttttaga
     2221 tttttgtatg cctctcgcag gctttttcga agatttttga gcaggagcga cactcactcc
     2281 ttgtgctttt agtgttttta attcttgctc taactgttcc atacgttgca acaatacatc
     2341 tgtgtttggc gatgaagcaa tttgtgctgg ttcagctaca ttcgcaatca cttgtggttg
     2401 acccttaatc tgctcagcta attttactaa caacacttca aaatgaacgt tttgattcac
     2461 actaaaacga atcgacacta atgtatcatt aataagatca atcatttgat ataacatatc
     2521 taattctaag ttcatcagtg ctcgatactc agtatctttc tcagatgttt tattcataat
     2581 cgtatctctg acaaaataaa tcatatcatt tattaggcga ttcacttctt taccctctgt
     2641 tataaactga tggtattttt taaaagatgc ttgtacgtca ccttgtacaa tatcatcaaa
     2701 caagtgatcc aacgcttcat catgtacgct acctgtgaca ttcaacgcat cttgcaatgt
     2761 taacgtacca tcaccaaatg caatagcctg atccataata cttaatgcat cacgcatacc
     2821 cccttcagac gctttagcga taaatgccaa ggcttcatct tcacattcaa tttgttgtgc
     2881 atctgctaca aattttaaac gttcaacaat ttgatctagg ctaattgctt taaaatcaaa
     2941 acgttgtgcc ctagaaatga ttgttggagg gattttatgt ggttctgtcg ttgccaatat
     3001 aaaaatagcg tgtgctggag gttcttctaa cgtctttaaa agggcattaa aagcacctgt
     3061 tgttagcatg tgcacctcat ctataatata aactttatat ttcgattcac ttggtgcata
     3121 tttaactttg tctctaatat ttcttatttc atcaacgcca ttattactag cagcatcaat
     3181 ttctatcaca tctgaattag tcccctgcgt aatgccttta caaatatgac attcattaca
     3241 aggttctcca tcagtgctat ttagacagtt gattgcttta gcaaacactt tggcaatact
     3301 cgttttcccc gtacctctcg gaccactaaa aatataagca tgcgactgtt tttctttcga
     3361 aatcgcattg cgcaatgtct tcgtgacatg ttcttgtccg acgacatcct cgaaactttg
     3421 gggtctgtac atacgatata aggcttgata attcaagtta gcacctccat aaacaattac
     3481 ctctcattat agcatgataa tacctttact tcttaattga acaattaata aaaatgttct
     3541 ggaaatttta cgattccgtg tggtgcatcc gtcaaattat cccataaata ttttacaagt
     3601 aacggtgcat caccagattc taatttaatg tcatgctcag cagcattttg ataacccaac
     3661 ttttcaaagt agtcaaaaca atggtctaca acaaccgtac tatactcttg tgctttggca
     3721 cgctcttcta ctgcttgaac caagccacga cctaattttt gtccacgtaa ttcaggatga
     3781 actgataaag aggcaatcgc caaaccataa tacgtcttat catcactatt aatttctact
     3841 tcaattaata aaacgtgtcc aacgacatcg ttattttcat ttttcgctat tacttctaat
     3901 tcaaaattat agcaaggaga ttttcttaaa tgttttactt tcgcacgtgc ttgccaactc
     3961 gtttcaggat tatcatcaaa actttcttca atactattta aagatttatc ataatctaac
     4021 tctgttaaag tacttaaata tatttgcata tgtcctccgt aggcatttga ttgtcaataa
     4081 tcataacgta tctcattaat aattctattg taagatactc cccctcattt caccatcatt
     4141 tctttatcat caaattataa cttcttactt ttcattgaca caaaaatcat tcaaactgca
     4201 catcgagttc acttgaatca aacttcacat ataaaaaagc tacttcccac aaacatgttc
     4261 cacgtataat acgctgaatt gtcttcaaga aagtagcttc tataaataaa tatatatttt
     4321 caactcttaa taatcggttt aatatttaaa aaataaaaaa ccgtgcacct aagcatcgaa
     4381 cgtaattcac gaacgtaacc aaagtcgtta gctaaatctc ggctacccta cggcacatat
     4441 gatgatccac ttaatgctgc ttccgtcagg acctgacatg gttcatgggt tcatattgca
     4501 taggaccgaa atcttcaaac actacgtgct ttgggcagac ttcgcaaaaa tacggcctca
     4561 acaaaggaat taagcctcgc ataaagcgga tttcgagtac agggaaccgc tacctcccca
     4621 cctagcacgg caagatatat attactatat tttaatagtt aattgcaagt ataaatcatt
     4681 tatatcattg tttactttat acgacgtctt gagaagtcgt taaatttaaa ttcatttgca
     4741 agatgttttg aaatattata ttgaaacggc attgtatttt ctaaatacac aatacttcga
     4801 actgttgctg aataagccac cgatacatca ccaaacaatt gatatgcttg ttcatcaaac
     4861 ggttcaaaag taatagactt acttgaataa ctaatattaa gatttaatac cttttccaaa
     4921 taatcataaa tagaatcact cgcaacatca ttacctatat ctgaaacaat cgactttaga
     4981 aaataatctt catcaacaat cttcacatgt tggttaagcc gacgagttct aacaatatga
     5041 atgagcgatt cactagaatt gatgtttaaa gcatgttgaa cttctggaac ttcatgcgct
     5101 tcaacaacct cattcacaac aacttcagtt aaatatgcga cgcccatttc ttcttgcatt
     5161 tctttaaaac taacaagttc agaaaatgga aactctgtaa cctcctgata aatgacaagt
     5221 gaccctttac catgaatctt ttgaatcatg ccgtctaatg ccaacaaatc taatgcctta
     5281 cgcacggtct ctcgagatga ctggtacaat tgcaccaaat catgttcaga cggaatttgt
     5341 tcaccatatt gaatctgccc gtttaatata tcttctttca acgcctcata aatcttcata
     5401 aacttttttt gtttcgccat ataaacgcat ccactcaatt tattttacgc gtttgtttcg
     5461 attacaattg caccaaaccc gcttatcgta ttattttcca ctgtgcctgt ttgaatcaca
     5521 acacaacctt ctctagccaa tccttctggc aaatcaacag ccgatgctga gaaattcgca
     5581 attactagcc attgttgatt cttataatga cgttcataaa caaataaatg atcatgatcc
     5641 ataaaacgtg gcacaatgtc tccatacgta ataatatcat gcgtatgtct taattgtatt
     5701 aatttttgat acgtatagaa aatagactct ttattctgaa gtgcttgtct aacgttgacc
     5761 tgatgataat tttccgaaat atcaatccaa ggcttaccag ctgtaaatcc agcattaaca
     5821 tcatcactcc attgcatagg cgttctcgaa ttgtctcgtg acttctgtcc taaaatcgct
     5881 aatacatccg cttcagcatg cccttcactt agcaactgat ggtaggcatt aatcgattca
     5941 acatcacgat attgtgctat tgatgtaaaa tgtgggtccg tcataccaat ttcttcacct
     6001 tggtaaatat atggcgtccc ttgcaacata tgcagtgcga tagctaacat tttagcactt
     6061 tgtatcctca tctcttccga cgtatcatca ccaaatctag acactacccg tggctgatca
     6121 tgattacacc agaaaatcgc gttccatccg ccaccgtcat aaatacctcg ttgccattgc
     6181 atcagaattt cctttaactt atgaaaatca agcttcgcat ttgtccactt ttcaccatca
     6241 acataatcaa cctttagatg atgaaaatta aaaacactat tcaattcttg gcgttctggt
     6301 tgtgtatact taatacaatt ttcaatcgtc gtcgaagaca tttctcctac agtcatcatg
     6361 tcagtgttac caaacgtttg acgatttaat tcatgcagaa actcatgcac tctaggacca
     6421 tccgtataaa attctttacc tattttgtca gagtccttaa attcaccttt agaaattaag
     6481 ttaatgacat caaatcgaaa accatcaacg ccgaagtcta tccaatgatt gactatgcga
     6541 tataacgatt gacgtacttc cggattatcc caatttaaat cagcttgact gacatcaaat
     6601 aaatgtaaat aatattcatc tgtctcagaa tcatacttcc atgcattacc accgaattta
     6661 gaatgccaat ttgttggcgg cccgtcttca gatgatctga aaaagtaata atctctataa
     6721 gggttatctt tagatttacg ggcttcttta aaccattcat gctccgtcga cgtatgatta
     6781 ataacaatat ctaacatcac tttcaagtct ttttgatgag caactttgat taacttttca
     6841 aaatcatcca tcgttccaaa ggcttcattg atttctaaat aattgctgat atcatagcca
     6901 ttatcattca taggtgattc atacactggt gttaaccaaa tataatcaac acccaataac
     6961 ttgatataat ccaatttttc tataattccg ttgatatctc ctataccatt ccccgtcgta
     7021 tcattaaacg acttaggata aatttgatat acaacggatt ttctccaatc tatttctttc
     7081 gacacacaat taccacctta taacgtaata aaggacgtcc aaataacgaa cgcccctttt
     7141 ttattttatt aatcttcaac aatttctttc gctttttgtt tactaaaatg agacatcaca
     7201 attgttagta tacatggtac aacaatagca atagctgtca caataagata tactggccaa
     7261 aattcttttt gaattgaaat gaatgctggc acgccaccaa caccaacttt tccaagtacg
     7321 ttattcatac caacgattgc ccccaataca caagacgttg atatcgcagc gataaatgga
     7381 tatttcaaag gtaagttcac accaaacata gctggttcag taacacctaa cataccagaa
     7441 atacaagatg ttaatgccaa gccttcttct ttaaccattt tacgacgttt atagacaaac
     7501 catgctccaa atgctgcaga gccctgacaa atattggaaa tcgcaacaat tggccataaa
     7561 tacgtaccac ctaagctgct acccatcaat tggaaatcta ctgctaaaaa catatggtgt
     7621 agtcctgtaa ttacaagtgg tgcatataac aatccatata ttgctccgcc aagccatcct
     7681 gcatgttgga atataaatgt aacaccagat gtaatacctg taccaatcaa taatgcaact
     7741 ggtccaatga taataaatgc taaaaatcca gtaactaaaa gcgctacggg tccaacgacc
     7801 aacattttta tcgaatcgtg aacgacttta tttaatcctt tttcaatttt agctagaaca
     7861 taagctgcaa ttaaaacagg caacacttga ccttggtaat tcaactgctt aatctctaag
     7921 ccaaataagt tccacgtcgg aatattccct tttgccaaat catactgaga tactaattgc
     7981 ggatgcatta aaatcaaacc taagactatg cctagaatcg gactaccacc aaatacacgc
     8041 atactactcc aaccaattaa tgctggtaag aaaataaatg ccgtactcgc aatcacatta
     8101 atgatgtttg aaatatcagc aatttgcgga tacatctcaa taagtgcttt tggaccaaat
     8161 aaacctttca ttgtaagtaa attattgatc cccattaaca aaccagctgt cacaatcgca
     8221 ggtaatattg gtataaaaat atcccctaac aatttgatca aacgttgtac tggattccct
     8281 tttttcgcag ctgcttgttt cgcttcatct ttcgaagctt cttgagcacc tgtttcatca
     8341 ataaactgct tatacacttc atcgactgtt cctggaccaa tgacaatttg atattgatgg
     8401 tctgctttga actgcccctt gaccaacgcg ttattactta atgcgtcttt atcaacttta
     8461 ctttcatctt taagcactaa acgtaatcgt gttacacaat gcgttgcagc ttcaagattt
     8521 tcttttcccc caatagcgct tacgatggct tttacatctt ctctttttac agccatatat
     8581 ttcactccct taatgtctta aaacgagtat aacttgtcta gacaagttat gtaaaggctt
     8641 tcaacaaatt atttttattt tgtcacatga tctgatacag ctattttaga aaatcaatag
     8701 gccataacga catatgtagc aataactcct cgctacaatt gcgtgattaa caatacctca
     8761 ccacgctctc accattataa agtgtagatg catggccaac tcctaaattg aaaaatgcat
     8821 atctataaat agcaaacttg cccacacaag ccatccatca tatttcacaa aacaaaaaag
     8881 acatttagct atgtatattg cacaagccaa atgcccttcc aatctcaatg ccctatcatt
     8941 tctatagcta aaaaaacgag ccattcaaag acaaataaaa aaggcaaata ctcaaaattt
     9001 gagcacttac cttcattata tatagtattt aatataaata ttgatggcgg aggaagaggg
     9061 attcgaaccc ccgcgagccg ttaagcccct gtcggttttc aagaccgatc ccttcagccg
     9121 gacttgggta ttcctcctaa acacaatatt tatcatatat gaacgttctg taaaagtcaa
     9181 tattgttttt aagcattaca tgattaccac aaatcattgt cttccactac agttgaccta
     9241 taaacgaatg taaaaaatca ctacttataa ttaatcgatt tgttgtctta caaacctttg
     9301 tttttaggtt acaaagatgg catgtgacgt tgatagatac tagattattt caaactcgat
     9361 tcagcatgac acaacgtcaa cgtaaccacc atttccatac aaagattata cacaaacttt
     9421 actcgctaaa tactgatcta ctgctttcgc tacgcctcta ccttctttaa ttgcccatac
     9481 aactaaactt tgaccacgtc tagcatctcc agcagcaaat accttttcat tattagtttg
     9541 atagtttgta tcatccgcca cgattcgatt tctatccgtt ttaatgttaa aagcattcgg
     9601 tactgttggt tctgtacctt cgaagccgat tgataataat acaaggtcag caggccaaaa
     9661 tctttcaggt ccttctttca tgaccatacc attttcgcct tgctctaaaa tttgagtata
     9721 caaaccacgt atgtgtcctt tatcgtcaac atcgtaacgc attgtttgaa caccatatgc
     9781 acgtggttcc ttaccaaact tagcttcgta ctcttggtgc gcatagtcca ttttaaacac
     9841 cggcattgct aaaggccatg atgcattttc tgtaaatgta attgcttctg gcaatttcgt
     9901 atatttatta aattgaacaa tcgatttaca attttctctt aatgctgtcg ctacacagtc
     9961 tgcccctgta tcaccagcac caatgataat gacattctta tcttttgcag ttattgtaat
    10021 atcatcaatt tctccattta acagctgcgt ttgttcagtt aaataatcca tagcgaaatg
    10081 tataccatca cccatgcgtc cttctaaagg taaatctcta cctttttgtg caccagtaca
    10141 taatataatg gcatcatact cagattctaa cgttgcttta tcaatatcaa cgcctacttc
    10201 aacaccatta atgaaagtaa tgcccgcttc ttccattaac ttaatacgac gtcgaaccac
    10261 atctttatca agcttcatat ttggaatacc atacattaat aaaccgcctg attctctagc
    10321 acgttcataa atagtcactt gatatcctag tagattaagt tcttcagcag cagttaatcc
    10381 tgctggaccg cttccaacga ttgccacttt ttcatctcta cggcgactcg gaactttcgg
    10441 cgctacccaa ccattttcaa aagcttcatc aataattgtg cgttcaatac ctttaatcgc
    10501 aatcgattct ctattaatct tcatcacaca agcactttcg catggtgcag gacatacacg
    10561 ccctgtaaag tcaggaaagt tatttgtttc gcttaagcgt tcataagcag ttttaaaatc
    10621 ttgatgatac actaagtcgt tccattcagg aatgtagttt ccaattggac aacctattgt
    10681 ttccctacca tactgttgtc cggtttgaca aaacggcgtt ccacaatcca tacatcgcgc
    10741 accttggata gaggcatctt ctttagtaaa tcgttgttga tatgctttat gatgcttcaa
    10801 acggtctacc agtgataatt cacctaagta ctgtttgtca tacttcataa atcctttaaa
    10861 ttcacccatc gtatctcccc ctttccttaa tacacaacgg ctggtttatg tttagcgtcg
    10921 attgttttac tgtcatcgta aaatgcagct aacatcgctt catcttcatt gtcatgtaat
    10981 gatttgtgca aatgaatttt ttgcatcatt aattgataat ctttaggaat aactttaacg
    11041 acgacatctt caatgcgatc aaaatgtttt aacacatgaa tcgctctcgt actattcgtg
    11101 tgtgacacat gttcttccag catttgctta atgaatgctt tttcttcttg gtgtttaatc
    11161 tttgtaaacg aaagcgtatc tagttgatta ttttcaacaa aagcttctac atcagacggg
    11221 ataacgtaag caataccacc actcatacct tgaccgaagt tcttacctac atcacctaaa
    11281 ttaatgacat gtccaccagt catgtactct aatccatggt caccgatacc ttcaacgaca
    11341 acatctacac cactatttct aatacagaat ctttctcctg cactaccgtt aataaatgcc
    11401 ttaccacctg tcgcaccata gaatgagacg ttaccagcaa taatttcatt ttgtcgttct
    11461 tcaaaaggtg ctttgacaat gaccgtacca ccagataatc ctttaccaac atagtcattc
    11521 gcatctccag tatgatgaat cattaagcct ttcggtgcat atgctgcaag actttgacca
    11581 gcatgaccat tcgtataaac attaattgta ttttcaggaa gtcctgcttc tccatattgt
    11641 ttcgaaatct cactacctgt aataactcct acatcacgtt gttcattatt tactgtaaag
    11701 ctacctgtat agcgacgtcc ttcagcaata tatggcttcg ttacttcata taaatttgtt
    11761 aaatcaaatc catgctcaag attatgattt tgttgaattt cttttgtgtt tggcccatcg
    11821 aaaggacata acagtttttc aacatcaata ctagccgctt tgctattcgc ttttaatgtt
    11881 gatgatcgtt gtaataaatc agttcttcca actaagtctt ctacacgttt caaacctaaa
    11941 gatgctaaaa tttctcttaa ttcttgtgca ataaaatgca taaaattaac aacatgatgt
    12001 gctttacctc tatataaagc acgtaaatct ttgttttgag ttgcaactcc tactggacat
    12061 gtatctttat ggcatacacg catcataata cagcccaaca ccactaatgg tgcagttgca
    12121 aatccaaatt cttccgctcc aagcgcacat gcgtacgcta catctttacc agttaataac
    12181 ttaccgtctg tttctaactt aacacgactt cttaagtcat ttagttttaa tgtttgatgt
    12241 gtttctgcta aaccaatctc ccaaggaaca ccggcatgct gaatactcgt tttaggtgaa
    12301 gcccctgtac caccatcgta accactgatg acaattttat ctgcaaatgc ttttgccacc
    12361 ccagatgcaa tggtaccaac acctgttttc gaaactaatt ttaccgcgat atctgcatct
    12421 ttattcgcat ttttcaaatc atgtatcagt tgcgctaaat cttctattga ataaatatca
    12481 tgatgtggcg gtggtgaaat cagaccgata cctggcgttg accctcttgt ctttgcaatc
    12541 cacggatata ccttagtacc aggtaattga ccaccttcac caggctttgc accttgcgca
    12601 actttaattt gaatttcttt ggcatgttgt aaataatcac tagttacacc aaaacgccca
    12661 gaagcaactt gtttaatcgc acttactttg ttgcttccat caacttgtac ttcataacgt
    12721 tttgcatctt cgccaccttc accactatta ctctttccac ctaattggtt catggcttgt
    12781 gctaacgttt catgtgcttc cgctgaaatc gatccataac tcatcgcccc tgtattaaag
    12841 cgtttgacaa tgtcacttac cggttcaact tggtcgatgt caatcggtgt acatgcttta
    12901 aattcaagta aatgtctaat gtgatctgtt ctatttttgt tcaccgcttc agagtatgct
    12961 ttaaattgcg catagtcatt ttctttacat gcgtgctgca ataagaaaat agattccgga
    13021 ttaaaagcat gatgttgacc ttgttgtctc cattgaaatg tactacctga tgcaagataa
    13081 ttatcatcac tttgttgacg tgctttattt tcagcatcaa tttgatcaat cgaaataccc
    13141 gataacttag actgtgtccc agtaaaataa cgatcaatca catcatgaga caagccaatc
    13201 gcttcaaata tttgtgcacc ttgataactt tgcactgtcg aaattcccat cttagccatt
    13261 actttaatga caccttctga caatacatcc gtatatgtct taacattatc gacaacggtg
    13321 ccttgtaacc cttctgtcaa tgtcagttgt tcaactgtac gttgcgctag gtatggcaca
    13381 attgcattcg cgccatatgc gagtaaacaa gcaacatgat gcacttctcg tgtctcacca
    13441 gatttagcga ctaaacttgt agacatacgt aaatctgctt taataagtaa ttgatgcaca
    13501 tgacttattg cgagtaacat cggcattgca aagccattgc tatcaactaa tccactgtca
    13561 tctaacacta gaatttgagc gccttgcttt acagcattca ctgcttctcg gcctaatgct
    13621 tctaacgcat cttccaaatc cccttcatat accgttgata aataagttaa tttaaaatgt
    13681 tcctgatcaa tcgctgctaa gtgtgattca ttcaataccg gccttttcaa ttgaatacga
    13741 tctaaaaccg tttcgtcagg tgctagtaag ttaccttcgc cacctaaata agaaagttca
    13801 ctcgttacga ttttttcacg atacgcatca attggtggat tcgtaacttg tgcaaacagc
    13861 tgtttaaagt aattaaatag tgattctggt cgctcgttca acactgcaat tggcgcatca
    13921 tatcccattg caccgatagg atccttctta ccttctacaa gttcctgaat atacttatga
    13981 atctcttctt tcgtgtatgc aaactgacgt tgtaatttaa ataacgtctc atctttccat
    14041 tgcgaatctt gatattgtat attttcaaaa tcaaagtcat ctttatggtt atcaatccac
    14101 gctttatatg gtaattctcc agcaatcgca ccttttaaat cattattttc aatgacttta
    14161 ttctgtttaa aatcaacaag caataacttt ccaggattca attgaccttt aaaagcaaca
    14221 ttactttcag gtacgtccac aacacccact tcagatgaaa agacaataaa gttatcttta
    14281 gtaatcgtat aacgacctgg acgtaatcca tttctatctg taagcgcgcc aagtttgtca
    14341 ccgttacaga acgaaatcat tgtaggacca tcccacggtt ccattaaata actataaaat
    14401 tcataaaacg cacgtacatt tgcatcattc gcttcattgt ataaccaagg ttcaggtatg
    14461 agtagcatcg ctgccttttc tggctccatg gctaacgata agaactctag cgcattatct
    14521 acaatagcag agtcactacc atcctcatcg acaatttgaa acactttatg ttgatcctcg
    14581 ccaaataatg tttcgattaa tttatgttgg cgtgctcgca tccagtttac attaccttta
    14641 atcgtgttaa tctcaccatt atgcattaac atacggttag gatgtgccct tttccaactc
    14701 gggaatgtat tcgtactaaa tctcgaatgc actaacccta gctttgattg atataaatca
    14761 tccgataaat ctgtatatag ttttttaatt tggtctgatc gtaaccaacc tttatataca
    14821 attgttttgc gtgataagct cgtaaaatac aattctaaat cgcactgagt cgaatagaac
    14881 tctaattgtt ttctcgctaa aaacaaacgc ttttcaacat cttcaatgcc cctaatatca
    14941 ataaacactt gttgaatgac tggcatcgta tctgctacat gtttagcaat ggcatcttta
    15001 ttaactggta cattacgata accaagaatt gataaccctt cgccttcaaa atatttttta
    15061 aaaactgctt catgttcaga acctaaaatg cgttctttgg aaaaaaataa ccccacggca
    15121 tattcacctt cacctgggat atcaaagtcc gttacatgtt gtttgaaaaa tgcaaaaggt
    15181 atttcagtca taatacctgc gccatcacca gtgatgccat ctgcgccgac cccgcccctg
    15241 tgatctaagc gtcgcaacat ttcaagcgat ttatcaatga tgtcgtgaga ccttttatta
    15301 tccatattcg cataaaaacc aataccacac gcatcatgtt cctcacgata gtcatataag
    15361 cctttaatta atttctcatt gtgcatgatg cacacccctt ttttagaata ttctgatatt
    15421 agttattaat gataattact ataatctaaa aaatttatct gttcaatata taaaatagaa
    15481 gatattcatc taaaaaatag aacatttatc aataatattg ctataatagt tacaaaacac
    15541 tcattttcgg aggtgcctat atcgctatgg aaattaaaca attaagatat tttatcgagg
    15601 tcgctaaaag agaacatatc tccgaaacag cacttgaact aaacattgct caatcagcga
    15661 taagccgtca aattacatta ttagaacaag aacttaatgt gtctttattt aaaaagcaag
    15721 gacgcaatat tacgctcaca tctgaaggga aattattatt taatgaagct ctgcgtatta
    15781 ttgaacattt agatagcacg attgaacaat ttcaaagcca tggattaact aaaaataaaa
    15841 gtatctacat tggctatgat gagagtgacg tatcccacat gttactacca cttatccaaa
    15901 cgtttcattt acaaaatgat acacatgtca taccgagctt attagatcat gacacgatta
    15961 taaatagcgt attaaatggg aatatagaca ttggtttcac ggaacttaca ccagaaatac
    16021 gtaaacacaa acaattacat atgctacctt tattcgaaga gcactatcat ttatatgcgc
    16081 cttcagacga tccaattacg atggcgacac acccaccatt aattcagttt gagcatagtc
    16141 atatttattg cttggcacca tttgccgaaa ctgtaaaaaa acaattgcgc aagattacta
    16201 aatcagatgt atacacaatc agtagccaac cacttgcgca gtatctcctt cgtcaaaaag
    16261 aaggttacat catttcatca caaaatatac atttaccaga atctaaatca tggatagata
    16321 taaaattaga tcatactgaa ttaaaaagaa cgatatgtgc tattacaaag gagccttata
    16381 caaagagtga tattggcata ttattaacac ttattcagca attaatgact aaaacgtcta
    16441 cattccatta agtaattcga tgctatcaat gatgattttc gttttacact tagcagtaat
    16501 atctttgaaa ataaaaaatt aagggcgtat aatcaccata ttaattcatt catagaattg
    16561 gggttttgtt tttgaaatat agaaatttat ttacaaagcc atttaatata attttaggtt
    16621 tgttctgcat cgcattcatt ggtgctattt tatttacatt caataatgag aacttttaca
    16681 acaaaccaat tggtcaaatt atagatgtca aacatgtttc ttcgacacct acaaaagatg
    16741 cacaaaataa tcgtgatatt aaatataaaa atcaattaaa agtcaaaata ttaaatggtc
    16801 aatttgctgg agaaacgacg acgattaatc atcagtacgt caaatcgcaa gcagattctg
    16861 aggcatttcg cacacacgaa aaagtattat tacatatttc taacaagccg agtgatgctt
    16921 atattataga aaagaagcgt gataccttaa cggttatcat cactggtttg ttcttattaa
    16981 ctgtattatt ggttggtcgc aaagtcggac tgcaatcgat tttatcgtta atcttgaatt
    17041 ctattgccat attaatcgca atttacattc atattcaaca tagcaatatt aatttattct
    17101 tactcatgac cattgccatg atctgttcaa caatactcac tttattgctc gttacaggct
    17161 ggcatatgcg tacactcatt accatagcca gtacaattat cggaactttt ttgagtatag
    17221 gtttaacaga attaatcata tatatgacag atggtaaagg cattaaatat gaaacgatga
    17281 actttttatc attaccaccg aaagatatct tcctagcctc agtgttaata ggttcacttg
    17341 gcgctattat ggatgttgca attacaattg ctagtggtat gcatgaaatt ttacagcgta
    17401 cgccacacat ttcaatgagg agatgggcgc ttgctgggcg aaacatcggc caagatatta
    17461 tgggaacaat gactaacatc ttattattct catatttatc cggtgcacta cctatgtttt
    17521 taatcttttt aaaaaatgca aatactgtga cttatactat ttcgatgaat tggtcgttag
    17581 aaattgcgcg cgcactaaca ggcggtatag gtatcgtact tacaattcct atcacaatat
    17641 tgtttatgga aatatttgaa acattaagga gggcgaaaca atgaatgctg tatttatatt
    17701 ggcgttattg ctcctctttc tgatgataat tttcggaggt aaaaagggat tcatctctta
    17761 tttaacccta tttttaaatt tcgctatttt aattattagt attgtattga ttatttttgg
    17821 cgtaccgatt tatgtcgtca cattcttttt ctgcattatt attggcgctt gtaatttatt
    17881 cgtactcaat agttacaatg ttaaaacaca agctgcattt atcagcacta tcgttacaac
    17941 aatattgttg attacactca tttatttatc tgtctacttc ggtcatctac aaggcttttc
    18001 aaccgaacaa caagatgaaa catatgtgta ttcaatgaat attggcattg atatggttca
    18061 atttatggtg ttcactatta ttcttgctgt tattgcagct gtgatagatt taacgattac
    18121 cattagttca cccatttatg aattgcatca agtaaatcca acacttacgc aatacgaatt
    18181 attccaatct ggcatgcgtg ttggtagaga aattttagcg acatctgcga acacgattta
    18241 ccttgccttt tttggtggcc aacttgcttt atttatttgg tttttcaaac ttaaatattc
    18301 atttggtcat attataaact cgaaaatatt tgcccaagaa tttatttcta ttattttagg
    18361 cggtattgcc gttgcaatca gtattccgat tacggcttgg attacggcat ttttaataaa
    18421 aagaccatct cgacagcgca aaaatgatga gtctatcaat taatcgcaac tagcaatata
    18481 acttaagtta agcgacctgt ggatatatgt caattgttca ctaggtcgct ttttactata
    18541 ttaaattgtc cggtgatcta taacaaaaag cacctttatt catcattaat tgttgtggtc
    18601 gacgatgagt aaagatgctt ttaaatttaa tttattttat agttaacgcc ataatatgaa
    18661 caggtcttcc gatgaacata ctatctccca tatattgata tccttgttgg cgataaagtg
    18721 ccatggcatg tggattgtca gtattaaccg ttaacacaat ctcattaata tcctgaaatg
    18781 ttgaagtgat aaatgacgcc aatttttcca ttaccacttt acctattcct ctattacgat
    18841 aacgttgatc aacactaaat gacctgaaaa atactgcatc ttggttatcg ctaaatggtg
    18901 cgaccccttt tccacgatgt aatgtaaaaa aggccacaca ttgcctgcca tccattacaa
    18961 cgatgggata cctttcatta tcagacattg cagctgtaat attttcctct ggcgttttaa
    19021 caaacttcaa atcactttca cttaattgaa aagcttctaa catcgaataa tcttgtggga
    19081 cataatgtcg taacatgatg gtgtcattat ttgtcatgtt taccaccata cgtttcaatc
    19141 caaactttga cagcaggcgc aatcaacgca tcattatctt tatcaatcca gcgtatatcc
    19201 gtaatttcat tattgatagt tacgttttcc caatcgattt tggttaatgc gcgaaatcca
    19261 tttaactcag ttaacatatc ctgttgtgga tatgcaggac ctacaattgt cccgatatat
    19321 tcaatttcat ccattgttaa tgttaaattt aattcttctt ttacttctct taacagcgcg
    19381 tgtacttgtg attctccttc ttctatttta ccacctggga aataatactt ttcgcgattc
    19441 cttacttgaa caagcaatat tttatcagct gtttcttcaa ctaaacagac acatttaatc
    19501 atctttgaca ccccaacttt gtgaaatcaa tttttcaaat tatactgtac aattatgtta
    19561 tcatatatga gtagttatag cgcaaaacgt tagcaattca gcgcacccaa cttttcatat
    19621 aaacagaaga tactaggggg aattattatt atggcaaaac gttccaaatc acaacgttta
    19681 tcaagtttac taaatgtcgc aggtttcata gtcgacggct acaatggcta taaatatcat
    19741 gctaaaaata aaaaattagt atatctttca ttaggtttaa gcactgtagg aaccgtgtta
    19801 gacttttaca tttcaattaa gtcaccacgt aagttcaaaa aagcagtggc agttgttact
    19861 ttaataacaa acggtgctag attatttaca agcattcgca aagtaaagca tgaatactaa
    19921 ttcagaaaag gattggtcga acatagaaca tgaagttcat tcgaccaatc ctttttctta
    19981 tacaaaaatt cctattacaa caatcacgac tttgatagcc ccgccataaa gtttaggatt
    20041 caatccaact tttcagcttg tgaaatgtaa taggaattca ttatatattt atatacgtaa
    20101 gactttagtg aatatatcta taattattta cttggtaagc tggtaccgtt ctgtaagtta
    20161 aaatacctgg tgcagctgaa tagttcattt ctgaaactaa aatactacca tcgttattta
    20221 cacgttctac aaacataaca tgaccataat aacctacatc tgtttgagcg atagaaccta
    20281 cagtaggtct attgtcgata gtgtaaccat ctgctgccgc tgcgttatcc cagttattag
    20341 cattccacca ataagtacta atacctttac caatttcagc acgacgatta aatacatgat
    20401 atgtacattg accccatgta tataagtttt ggtgactgaa tactggtgta ttgtaacctc
    20461 tattcgtagt tgttgcagat cctgagttcg tagatgcatt accagttact ttcaattttt
    20521 gacccggata aataaagaaa ttatttaagc catttaagct cataattttt tgataagttg
    20581 taccatattt tgatgcgatt aatgacaatg agtcacctgc ttgtactgtg tagtatgatc
    20641 caccgcctga gttcgttgat ggacggctac tattgctcgc agcgttactt gagctagcag
    20701 tacctgatac ttttaatttt tgacctggat aaataaagaa attatttaaa ccattaagtc
    20761 gcataatgtt ttggtaagtt gtaccatatt ttgatgcgat taatgataat gagtcgcctg
    20821 cttgtactgt gtagtatgat ccgccacctg agttcgttga tggacggcta ctattactcg
    20881 tagaattact tgagccagat acttttagta cttggtttgg gaaaattaga ttagatgtta
    20941 aattgtttaa tgactttaat ttagcaatcg aaatcccata cttatttgaa attgcccaca
    21001 ctgattcacc cggttttact gtgtgagttg tagccgcatt tgcttgagtt gccgcaacag
    21061 cgctaatcgc gcttgtccca ataatagctg caattacttt ttttgcactt taaaatcctc
    21121 ctcttgctta actttcctaa cattctttta tccgaattta tgaatactac atcattatac
    21181 gattttatta tgtataatag gttgatgttt gatgacatta tgattacaaa aaaatcatat
    21241 actgtatcat caaattttat aattacccct taaaattatt acaacttatt agattttaca
    21301 atatctaaat tattacaatt tcataatatt tcactataaa atgattacat tccctttctc
    21361 tattggaaat aattttattg tcaaacaata acgccctaca aacataagca tgaactttgc
    21421 ttgtaggacg caatataatt tattttgcta atgacgtttc tattgcctta atctcatctt
    21481 tagataaatt aacaggtttc tctccatctt tgacatcttc ctgcaacgct ttttgagctt
    21541 cttttgaatg atacaattct acgattttag catatgtttt gttatccaag tctttgtcat
    21601 taactgcaac aatattaata tatggcttta cagcatctga atttgatttt tctaaaaata
    21661 tcggatcatt tttaggatct ttacccgctt tagttgctac accattatta ataactgcaa
    21721 tatcgacatc agataaagca cgcgcagttt gttgtgcatc tactgcagta atttttaaat
    21781 gttttggatt tgacgttata tctttcactg tacctgctaa tccgaaatct tttttcagtt
    21841 ttattaaacc agctgcttct aatagtttaa gtgcacgtgc ttggtttgat acatcatttg
    21901 gaatgacaac tttagcacca tctttaacct ttttgacatc tttaatttta tctgagtaaa
    21961 tgcccaacgg tgctaaaact gttgtactca atgctgaaat ctttgttcct ttatgtgcct
    22021 tcttatactg atctaaaaat gcaaaatgtt ggaatgcatt catatcaata tcaccatcgt
    22081 ttaatgcttt attcggtaaa ttgtaatcgg agaagtgctt aatctccaca tcaatatcat
    22141 cttttttagc taattcttta accttctccc aagccttagt gtcatttgat gcgacaccaa
    22201 ttgttacttt tttatcattg ttactaccac acgctgctaa taatacaagt gctacgataa
    22261 ctaacccaat caatcttttc attctatcaa ttcctttcaa aatcttcact atatatcatt
    22321 aatgtctacg tatgaatcta gctaggacat tccctagcgt ttgaatcact tggacaataa
    22381 tgactaatac aataacggta ataataatga ccgtcgtatc aaatctttga taaccataca
    22441 ctaaagctaa gtctcctata ccaccaccgc caacagctcc tgccatcgcc gtacttccaa
    22501 taagtccaat aatcgcagtg gtaattgcta atactaatga acctaacgct tcaggaatta
    22561 aaaaatatct aatgatttgt agtggtgaag cgcccatcgc tttcgccgct tcaataatcc
    22621 cctcgtctac ttccaataat gagttttcaa caagtcttgc aatgtaaggt gccacatata
    22681 cagttaaagg cacgatggca gcagtcgtac caattgaagt acctactact aattttgtga
    22741 atggcacaat cgcaattaac aaaataataa atggtagtga ccttaaaata ttgattaaag
    22801 gatttaaaac ttgatgtatc actatattgg gccatatgcc ttgttttcga gtaattacca
    22861 ataagacacc taatggaata ccaatcactg ctcctaaaaa taaagcaata gataccatat
    22921 ataacgtttc gtacaatgct tgtaataact gtgcactgtc taaatcagaa ccaaacatat
    22981 gttaatgcac ctcctcaaat tgaatatttt tctctttgaa atattgattt attgccgtgt
    23041 catcaaattg ttgatctata ttaaatcgaa gccacatata acatacggtg ttaccttgta
    23101 tttctgacat agatgaaaat aaaattttaa cctctctgcc acaaatttga atcaagtcat
    23161 ttataatcgg ttgtgtcacc tgagtttcct cgacgaagat tttataatct ttaaaaccgc
    23221 caacttgttc gtcattcaat cgacgaatca atgatgtact tggctcagtc tgtataactg
    23281 tagacacaaa attttgagca atcgtcgttt taggatgact aaacacctct ttaacagttc
    23341 ctgtttcaac cacttgcccc ttttccatta cagcaacacg attacaaatg tctttaataa
    23401 cgcgcatttc atgtgtaatc atcataattg taatgccaaa ggtttgattg acattcttta
    23461 ataacgtcaa tatcgaagca gtcgttgctg gatccaatgc gcttgttgct tcatcgcata
    23521 ggagtatttt cggattagta acaagcgctc ttgcaatagc caccctttgc ttctgcccac
    23581 cagataattc atcaggaaat tggtcttttt tatcactcaa tcctacaaat tcaagcattt
    23641 ccgttactcg ttgcttaatt tctgttttgc ttttcttact taaaatgagt ggcattgcta
    23701 catttttaaa taccgtagct gaatttaata aattgaaatg ctgaaatatc ataccgatat
    23761 ctttcttaat ttctcgcatg cctttttcgc tataattcgt aatatcatgt ccatctacaa
    23821 tcacttgtcc attcgaggca gcttcaagat gattcacgag tcttaccaac gtactttttc
    23881 ctgcaccact atatccaatc acaccaaaaa tatcattgcg attgaccgta aatgatacgt
    23941 ccttcaaagc atctattgtt tactttttct tatgaaatga tttagtaact tgtcgaaact
    24001 caatcatcat cgcccctcct acaaattctt attaatccac taggaataag agcattatat
    24061 gtaaaattgc atatatcgtc aatacaattt gccgaatttt ctaaaaaatt aaaaaaataa
    24121 ttaattcatg tgacaatgat gaattgtgag actactatga catttatcaa attaaatcca
    24181 tacaaatgtc caccaaacct ccataactca atcacgaatg attaacatcg cacaaaaaag
    24241 cactagcatt ttcaaaatca atttaagttg atcttaaaat atatgctagt gctgctatta
    24301 tttatagcgt atctagtgct tgttttaaat catcgactaa atcttcagta tcttcaatac
    24361 ctacagaaat tcttacaagt ccgtctgtaa taccttcttt agctcgaata tctgctggaa
    24421 tggatgcatg tgtcatcaat gcaggtactg aaattaaact ttccactgca cctaaacttt
    24481 cagctaatgt gtaatacgat gttgctttaa tcaattgttt ggcactttct gtatttttca
    24541 cttcaaatgc aatcacacct gtatggccat ccgcttgagc catatggaca tcatgattta
    24601 aatgactttc aatacttgga tggaacactt gttgcacagc tggatgtgct tgtaacattt
    24661 taataatttc aataacactg cgattaattt gttccatacg taaacctaat gttttaatac
    24721 ccctcacaag taaatagcta tcttgaggtc ctaaaatgcc acctgttgaa tttgaaataa
    24781 atgctaaacg ttctgcaagc ttgtcatccg atgttgcaac taaaccagca acgacatcac
    24841 tatgtccacc taaatatttc gttgcagaat gcaagacaat atcgatgcct aaatctaatg
    24901 gattctgata ataaggtgtc ataaatgtgt tatcaacgac tgaaatcaaa ccgtgttctt
    24961 tcgcaatttc agcagacttt ttaatgtcag taacacgtaa taatggatta gaaggtgttt
    25021 caataaacaa catctttgtt gttgggcgta tcgcttgtac aattgaatct gtatgcgttg
    25081 tatctacaaa atccacttca atgccaaatc gtgtaaatac ttttgtcaat gcgcgataag
    25141 taccgccgta tacatctgaa tttaaaataa tatgatcacc tttgtccaac agcataacaa
    25201 ctgcactgat agctgcaaca cctgaactaa acgcaaagcc atgtttgcca ttttctaatg
    25261 ccgcaataac gctttctaca gaacttcttg ttggattcgc agtacgagaa tattcatatc
    25321 cttgacgtaa atcaccaata tcatcttgta aatatgtact tgtttgataa attggtgtag
    25381 taactgcacc tgtatagtcg tccgttgtgt gtccaccgtg aattaattta gttttcttat
    25441 tcataatcat tctcctcata attaaatatt tgcttagaca tatatcgatc gctaccatct
    25501 ggaaatacga caacaatcgt accttcagat aattgcgctt ttaaattcaa tgcaccttgt
    25561 aatgctgcac ctgaagaact gcctactaac aacccttcat ttatagccaa acttttgaca
    25621 tttcgaaagg catcttgatc tttaatcgta aatatcccat ctacaagacg tctctctaaa
    25681 aatatcggcc atttctcaga accgatacct tcagtgtcat gtgcatgagc tggccctcca
    25741 tttaacacgg acccttctgg ctcaacggca taacattgca cgtgatgttg ctttaaataa
    25801 cgtgcggtac ctgtaaatgt accgccagag ccaataccag ccacaaaata atcaatttgc
    25861 tgtaatgctg aagtcaattc gggtcccaat gtatgaaaat atgtatccgg attatgttcg
    25921 gattcaaatt gattcatata aacggcacca tatttttcag cataggaacg tgcagctaat
    25981 tgtgccccat gcataccttc agactgactc gtccttgaaa catctgcacc aagcgctatc
    26041 ataatattaa tcttttcttc tgaaaaacca tacggcgcaa agatcttaca tttcaaatga
    26101 tgtctattcg ctgcaatagc taaccctatg cctgtattac cagcagtcgc ttcaacaata
    26161 gtttgacctg cacgcacacg cccttcttga attgccttct ctactaaata tttcccgagt
    26221 ctgtctttaa cactgcctcc aggattccat tgttcaagct tggcataaat tttaacttta
    26281 tcatcactat aatgttctaa cagtactaat ggtgtattgc caattaaatc ataagtaatc
    26341 atagatgcac cctcatctga catgccgatc aaatgaatga aacctttctt catgtctcaa
    26401 ttttaattct tacttttcag ataagaatta taaacgacat tttgttattt tgcaattatc
    26461 taagtttcga ttaattcaga accagtacta acttttcaat tccaaacaaa aaaacacctg
    26521 agcaacacaa atacttgtgt gtcagatgct tctatatatt aactaaataa ttgcacgata
    26581 aagactaaaa taataacgac aggcatcgca tacttaatta agtaatacca accattgaat
    26641 aatctaaatc gatctttacc aaaatattgt tgtaataatt ttttatctaa taattgtcct
    26701 atgacaagcg tagtacctaa tgcgcctaat ggcatcaata cattcgaaac gatgaaatcc
    26761 atattatcaa aaatcgttcc cgcaccgaat cttacatctt ttaagatacc aaaagataag
    26821 gttgctggaa tactaatgat aaatactaaa atactaccga tcactgcgac ttttttacgt
    26881 tttgtattgt cattcttcgt gaagttagaa acatttaatt ctaataaaga aatagatgac
    26941 gttaaagccg caaataagaa cagcactaag aatcccaaat agaataatgt gcctagatgc
    27001 atttgactaa agaccattgg cagtacttta aataataatc caggcccttc ttgtggttca
    27061 tagccaaaac tatgtaacgc cggaaatata gctaggcctg ccaatacaga tacaaagata
    27121 ttcataacaa cgatagaaat agctgatgac ttaatcgtca tgtctttaga ggcataactc
    27181 gcataagtaa tcatacctgt agttcctaat gataacgtaa agaatgattg acctagcgca
    27241 aacaagatgc catcagcagt aatctctgat actcttggtt gtaaaataaa tttcacacct
    27301 tctaaggcac catctaatgt taaagactta atcacaatga tgattaaaaa gacaaacagc
    27361 aatggcatca taactttcga tgccttttct aatccttttt caacacctaa catgacaata
    27421 atcatcgtag cgaatatgaa tataccttgc cctagaacag ttaaccaagg atttgatatt
    27481 accgcttcaa aattcatttc ttggagatga ttgatgcgtt gaaatataac taattgccat
    27541 aatacttgtc cgatgtaaat gacaatccaa ccaccgataa cactatagaa gccaaataaa
    27601 ataaacacgg ccaaattacc gttccagcca atgatattga gccatttttt accagttaat
    27661 ttactatata tttgtgttgt atatgtccgt cccattttcc caacagtgaa ttccataatg
    27721 agtaatggca acccaacaaa aatggtgaat attaagaaca tagctagaaa ggcaccgccg
    27781 ccataaatcc ctgccatata tgggaatttc cacatggcac caagaccgat tgcagaaccc
    27841 gcactagcta aaataaatcc agttgatgac ttccattgtg attgttgtct tttcatcatt
    27901 cacatctcct ttaaagcgtt aaagcgtcta cgtgtttaag taacactcta acatgtatgt
    27961 aaataacatg caatacacct gcatcaaaat tgtttcatta atacggtagc gcctgtattt
    28021 atcataatat agtcaactct tgtaatgtgt tgtcattacg ctacattaaa atttctggtt
    28081 caacgctgta tacgtgcgcc aatatctcgc cacataacaa cttcaggtac aatgcccata
    28141 atatttccac gacatcacac tacaaattaa ctttgtctaa gttccctata ctatcacgcc
    28201 acaaaaaaca ctcaaatgac aaatataata acatcgtcac tcaagtgtta atatttaaaa
    28261 tcttcaatta tgttaaagca ttgaaaaagc gaataacatt ttcttctaaa atatctctgc
    28321 cttctccatg tgtcattaaa tgatgcgaat ccttcagacc atttaattct ttgtctactg
    28381 atcctaaatg ttcataaata aaatgtgcac ttgtttcata ggatggctca tctttaccac
    28441 caaataatat attagctgga attttaataa catttaaatt tgtcatgata tcatcaataa
    28501 aatgctgaaa cttcgtaatt tcaccttcat aatcatcaat tgctgctaat tgcgcactcg
    28561 atgcttgctg atctaaattt aaaatattcg acatgcgttg actataataa actaaatgtt
    28621 ctattaagcc atcatcactt ttttcctttg gtgctgacat gacagcgata cgtttcaacg
    28681 ggtagtgctg cgccaatttt aatgtcatta atccacctaa agacacaccc gttgcactga
    28741 tagattcata accttcattg actaaaaatt ggtaagcttt ctcaacttct tcccaccaat
    28801 catctacatt atatgtcatg aaatctttca acagtaaacc atgacctgga taattcggtg
    28861 cataacaact aaatccttgg tcatttaact cagctgcaag atgcttcaca tcccgatttg
    28921 tacctgtaaa tgaatgtaat aataatatcg catgtccatt tgtgcctttt aaataagatg
    28981 gactcggtgt tttaattctc atttttctat atacctccac tatgtctaaa gatgtttgct
    29041 aaacgcgttg tcgtcgatga ttaataaagt atgggtatac cattaagaat aacgctaccc
    29101 aaatgattgc tagtgacgtg ccgccaatga catctgaaaa gtaatgtgca tgaaaataaa
    29161 ggcgacaaaa taatatgcta agccataata ttcccataac tagcgcactc aacacttttg
    29221 ttattgtctt agcagcaact gaaataataa tgatcattaa ggcgaaatat aataatgtgc
    29281 tggcgttgga atgtccactt ggaaatgaaa agcctgtatc aacggctaaa tgattatatg
    29341 gtcttggacg taaaacagta tctttaatta atttgttcat gatgacacct gaaaccaaat
    29401 atgtcacaaa ccaaaccgct aaatgcctct gtttaataaa cagtatgatt gtgacgataa
    29461 ttgaaatcaa cacgacacct ttgacatctc caatttccgc actaaacgtc atataataat
    29521 taaacaaatt gttaacatac tgacgttgtg gctcaccgaa ataatctgta aaccatgtta
    29581 atgatcccat atctatattt ttaagccatt cttgatttgt cactacaccg taaaacatac
    29641 ctataaatac aatcagcgcg attaaaaata aaggcactgt cattttcggt gatgttaatt
    29701 ttttatctat catcttacaa tctcctcgta tcatcatttt cattttacaa atgttatcca
    29761 taatatcaat gtgccacaaa tttcacttta ccgacaatat caaaattat
//
LOCUS       DAHHSC010000014        27765 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.014, whole genome shotgun sequence.
ACCESSION   DAHHSC010000014 DAHHSC010000000
VERSION     DAHHSC010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 27765)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27765)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27765
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.014"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            217..1017
                     /locus_tag="NBI57_000479"
     CDS             217..1017
                     /locus_tag="NBI57_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HCD1821457.1"
                     /translation="MVKAIAVDMDGTFLDSKKTYDKPRFEAIFTELRNRDITFIAASG
                     NQYAKLKSIFGDRDMYFISENGAVIYKGNELYNYKSFNRQVFQQVVDYLNMNQSIDQL
                     VICGLKSAYILKHTSEAFKEDTRFYYHQLKEIDSLQQLPEDDYVKIAFNINRETHPNV
                     DEEVATQFSNDIKLVSSGHDSIDIIMPNMTKGQALKRLLDKWEMSPSELMAFGDANND
                     KDMLAFAKHSYVMENSHDEELFNIASAVAPSNDKQGVLTIIEQEVLNK"
     gene            complement(1264..2256)
                     /locus_tag="NBI57_000480"
     CDS             complement(1264..2256)
                     /locus_tag="NBI57_000480"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000161541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HCD1821458.1"
                     /translation="MTKIMFFGTRDYEKEMALNWGKKNNVEVTTSKELLSSATVDQLK
                     DYDGVTTMQFGKLENDVYPKLESYGIKQIAQRTAGFDMYDLDLAKKHNIVISNVPSYS
                     PETIAEYSVSIALQLVRRFPDIERRVQTHDFTWQAEIMSKPVKNMTVAIIGTGRIGAA
                     TAKIYAGFGATITAYDAYPNKDLDFLTYKDSVKEAIKDADIISLHVPANKESYHLFDK
                     AMFDHVKKGAILVNAARGAVINTPDLIAAVNDGTLLGAAIDTYENEAAYFTNDWTNKD
                     IDDKTLLELIEHERILVTPHIAFFSDEAVQNLVEGGLNAALSVINTGTCETRLN"
     gene            2526..3197
                     /locus_tag="NBI57_000481"
     CDS             2526..3197
                     /locus_tag="NBI57_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HCD1821459.1"
                     /translation="MSNMNQTIMDAFHFRHATKQFDPQKKVSKEDFETILESGRLSPS
                     SLGLEPWKFVVIQDQALRDELKAHSWGAAKQLDTASHFVLIFARKNVTSRSPYVQHML
                     RDIKKYEAQTIPAVEQKFDAFQADFHISDNDQALYDWSSKQTYIALGNMMTTAALLGI
                     DSCPMEGFSLDTVTDILANKGILDTEQFGLSVMVAFGYRQQDPPKNKTRQAYEDVIEW
                     VGPKE"
     gene            complement(3318..4124)
                     /locus_tag="NBI57_000482"
     CDS             complement(3318..4124)
                     /locus_tag="NBI57_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCD1821460.1"
                     /translation="MAFHDKTATQVTNIELNVRDLDLMTTFYKNILGLSVKSSDDNTT
                     VLSVGTGGHTLTLHLLEDGRQTSPREAGLFHIAFLLPTTEDLANFLYFVAQKNMGIGA
                     GDHLVSEALYFNDPEGNGIEVYRDRPSSSWEWQNGKVKMDTLEVDSQTLLTHRTDEGW
                     QGMPAKGMIGHLHLKTHDLDAAYQCYIEQLGFQHVSDFPRALFMSTNHYHHHIAANTW
                     QSNQARQNNAQSYGLTHVDIYQPDASTHTFTAPEGFDITVHSNTDLVPEK"
     gene            complement(4295..4579)
                     /locus_tag="NBI57_000483"
     CDS             complement(4295..4579)
                     /locus_tag="NBI57_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCD1821461.1"
                     /translation="MSNLEIKQGENKFYIGDDENNALAEITYRFVDNNEINIDHTGVS
                     DELGGQGVGKKLVKAVVEHARENHLKIIASCSFAKHMLEKEDSYQDVYLG"
     gene            4805..5239
                     /locus_tag="NBI57_000484"
     CDS             4805..5239
                     /locus_tag="NBI57_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HCD1821462.1"
                     /translation="MDRTKQSLNVFVGMNRALDTLEQITKEDVKRYGLNITEFAVLEL
                     LYNKGPQPIQRIRDRVLIASSSISYVVSQLEDKGWITREKDKDDKRVYMACLTEKGQS
                     QMADIFPKHAETLTKAFDVLTKDELTILQQAFKKLSAQSTEV"
     gene            5317..6276
                     /locus_tag="NBI57_000485"
     CDS             5317..6276
                     /locus_tag="NBI57_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001628197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
                     /protein_id="HCD1821463.1"
                     /translation="MINNHELLGIHHVTAMTDDAERNYKFFTEVLGMRLVKKTVNQDD
                     IYTYHTFFADDVGSAGTDMTFFDFPNITKGQAGTNSITRPSFRVPNDDALMYYEQRFD
                     EFGVKHEGIQELFGKKVLPFEEVDGQVYQLISDELNDGVAPGVPWKNGPVPVDKAIYG
                     LGPIEIKVSYFDDFKNILETVYGMTTIAHEDNVALLEVGEGGNGGQVILIKDDKGPAA
                     RQGYGEVHHVSFRVKDHDAIEAWATKYKEVGINNSGIVNRFYFEALYARVGHILIEIS
                     TDGPGFMEDEPYETLGEGLSLPPFLENKREYIESEIRPFNTKR"
     gene            6307..6900
                     /locus_tag="NBI57_000486"
     CDS             6307..6900
                     /locus_tag="NBI57_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCD1821464.1"
                     /translation="MEHIFREGQKGAPTLILLHGTGGDEFDLLPLGEALNENYHLLSI
                     RGQVSENGMNRYFKRLGEGVYDEEDLEFRGQELLAFIKEAAERYEFDIEKAVLVGFSN
                     GSNIAINLMLRSEAPFKKALLYAPLYPVEVTSTKDLSDVSVLLSMGKHDPIVPLAASE
                     QVINLFKTRGAVVEEVWVNGHEITETGLTAGQQILGK"
     gene            complement(7005..8084)
                     /locus_tag="NBI57_000487"
     CDS             complement(7005..8084)
                     /locus_tag="NBI57_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001566820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1821465.1"
                     /translation="MRILKESIIVAFAFVGVVVGAGFATGQEIFQFFTSHGAYSISGI
                     IVTGLLITLGGMVVMHTGHHLKSRNHSDSINYFLYPSIARGFDIILTMFMLSLAIIMT
                     AGGASTIHQSFNLPYWLSALILVAFILATLFLKFDRLIAVLGGVTPFLIAIVIMIAVY
                     YFTTSHLDFTAANNDAQIHKQKSLSPGWWFDAINYASLQIAAAFSFLSVMGSKVKYRD
                     STLYGGLIGGLIITFLLMMINLGLISQFDKIKHVDLPTLKLATQMSPSIGIIMSVIMI
                     LVIYNTVVGLMYAFASRFSVPFSRRYFIIIITMAVITYISTFIGFISLIGKVFPIMGL
                     FGFILLIPVLYKGLIKRITGKSHID"
     gene            complement(8426..10390)
                     /locus_tag="NBI57_000488"
     CDS             complement(8426..10390)
                     /locus_tag="NBI57_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-1,6-bisphosphatase"
                     /protein_id="HCD1821466.1"
                     /translation="MTQITEKELKKKYLDLLSQNFDTPEKLATEIINLESILELPKGT
                     EHFVSDLHGEYEAFQHVLRNGSGNVRAKINDIFKERLSTKELNDLTALVYYPEDKLKL
                     IKSDFQSCGQLNVWYITTIEHLIELIKYCSSKYTRSKLRKALPKQYVYIIEELLYKSN
                     EYQNKKSYYETLVNQVIELKQADDLIIGLAYSVQRLVVDHLHVVGDIYDRGPQPDKIM
                     DTLINYHSLDIQWGNHDVLWVGAYAGSKVCLANLLRICARYDNLDIIEDAYGINLRPL
                     LTLAEKYYDADNPAFKPKKRPDKHERLTQREESQITKIHQAIAMIQFKLEIPIIKRRP
                     NFEMEERLVLEKVNYDTNEITVYGNTYPLKDTCFQTINRNNPAELLPEEEEVMNKLLL
                     SFQQSEKLRRHMSFLMRKGSLYLPYNGNLLIHGCIPVDENGEMESFEIDGHTYSGQEL
                     LDVFEYHVRKSFDEKENTDDLSTDLVWYLWTGKYSSLFGKRAMTTFERYFIADKASHK
                     EEKNPYYHLREDVNMVRKMLSDFGLNPDEGRIINGHTPVKEINGEDPIKADGKMLVID
                     GGFSKAYQSTTGIAGYTLLYNSFGMQLVAHQQFNAKEKILSEGIDELSIKRVVDKELQ
                     RKKIRDTNIGKELQAQIDILKMLMHDRYLD"
     gene            complement(10763..11533)
                     /locus_tag="NBI57_000489"
     CDS             complement(10763..11533)
                     /locus_tag="NBI57_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCD1821467.1"
                     /translation="MGTLIKQECFKLFKKKSTFIAPIVFILLMVAQGYIATKYNEIFT
                     PQESFTSAYNGFSWFAFLLIIQASTIISMEFHYGTIKNLLYREYSRTTMIVSKIITLF
                     IISLIYFVITIIASIVIGSLFFNDLNIFESSGNQLSLLNQLLLVSLGTFVGVWLVLSL
                     TLLLSSATNSTGVAIAVGIVFYFASSILAVIQTALLEKIDWLKWNPINMMNIMLQTVE
                     KGFSKSTKLELHELFIGNIAYISIFLILVVFIFKKKNI"
     gene            complement(11535..12230)
                     /locus_tag="NBI57_000490"
     CDS             complement(11535..12230)
                     /locus_tag="NBI57_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCD1821468.1"
                     /translation="MGVLTIEHLTKKIGNKTILEDVSFKLKRGQIVGLVGANGAGKTT
                     LMKVILGYSSFQSGNFNVINSKDSKSNIGALIENPGIYPFMSGYENLKLLNESKNTQD
                     IDKIVSQLHMDEYIHKKAKTYSLGMKQKLGIAIAFLNEPQFIILDEPMNGLDPKAVRD
                     VRELIVQKSQEGVTFLISSHILSELVKITNSILIINKGKIVTETSEEELKQFKDNDLE
                     NVLLEIIEREDQA"
     gene            12641..12740
                     /locus_tag="NBI57_000491"
                     /pseudo
     CDS             12641..12740
                     /locus_tag="NBI57_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501275.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(12749..13360)
                     /locus_tag="NBI57_000492"
     CDS             complement(12749..13360)
                     /locus_tag="NBI57_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCD1821469.1"
                     /translation="MEQIITEFISRFGYAAIFILILLENVLPIVPSEIILTFAGLMSV
                     KSHLSILTLFIIATIASFIGLLILYYICRLISEERLYRFIDRHGKWIKLKSKDLKRAN
                     DWFKKYGVWAVFICRFIPVLRVLITIPAGVNRMNVVTFTVISLIGTTIWNFGLILLGR
                     TLSDSFGMLMTGLHTYSRIMYVVIIIAVIYFAIRYIGKRKRVK"
     gene            13907..15184
                     /locus_tag="NBI57_000493"
     CDS             13907..15184
                     /locus_tag="NBI57_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002463344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCD1821470.1"
                     /translation="MKEKRTNVRWMFALAFFFIGVIAYMDRANISYIAKQMMDDLGMT
                     KPQFGLLASFFSLGYALMQVPSGMLAEKFGPRKMITIALVWWSAFTILTGMIKNHGLI
                     YLVRFLFGVGEAPMYPSNAVFNSFWFSKNEKGRASSALLAGSYFGPVLAPIVTIAIVN
                     AFNWQAVFYIFGAVGILMAVLWAIIAKDLPEQHRMVNEAEKRFIMENRDIVATEKSSP
                     PWNNFFKRFSFYAIAIQYFVVQFIITLFLIWLPTYLTEVFHVNFKEMSISSLPWLLMF
                     FLILSAGAISDRVLGLGRSKFVARGVIAIAGFIVFAVSIIFAVRTGNLYVSIFWLSLG
                     LGGIGISMGMSWAAATDLGRNFSGTVSGWMNLWGNIGALISPLLAGLFVEHLGWTMTF
                     QLLIVPAVIAVIMWFYVKPDQPLIVSDDKAIEK"
     gene            15661..17484
                     /locus_tag="NBI57_000494"
     CDS             15661..17484
                     /locus_tag="NBI57_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000586397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HCD1821471.1"
                     /translation="MIIPAIIGYLCGNFQFGLLVATGTLAHIYVFKGPSRSKLRTVII
                     CNLAFAICMMLGTLTAKTPLVFGMTLLIVTVIPFYIFTALKIAGPSSTFFIVTFSLPI
                     NLPIAPEEALYRGFAILVGGILATMMVLITIVFSKNKAEEQAIQNDFKLISKLLHTYN
                     DKAAFLQVAKTAVDSFKASDKLLITSTSSNDKLSRRFQKLLLLHTSAQGIYSELLELN
                     AKQIRPLPDELIEMMDHIIAQLDNSDENVRYWRKEVTVTEEFQNLFNHILKIDEMVHA
                     NEARIAYEADMRKPLYSKRIYQNLTLDSIVFRNTLRYTAIMMIAIFIALMFDFEKAYW
                     IPLSAHTILLGTSTIHAIERGMARGLGTILGVLVLSVILLFSIPTPVAVILMGIAALF
                     TEALVGANYAIAVVFITIQVILMNGLASQNLTINIAFPRVIDVAIGIVIAIIGLFVLG
                     QRTASVLLPNVMAEVVRKEATLFHYLFSENQYKDNVYQKNTAMNLSVKLNNMTQVYNA
                     ANGELFSDKTLIQNYYPSLFALEEISFMLNRAMANEDRLTINEQLMGEYLATFENIAK
                     HLELNTELEIKILPDLPQYNYIQSAMMNIQHNGFRERDKNV"
     gene            17623..18039
                     /locus_tag="NBI57_000495"
     CDS             17623..18039
                     /locus_tag="NBI57_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HCD1821472.1"
                     /translation="MPFTMKGYPEKWKEFEVIKRQKAIEIANAMLKEGYLEKDVVPIA
                     AKKAKDWYRALSKEEIKALEEENFVQFHTATMEKAEVDSEYATTDNEEHIQEQSNQDK
                     SYFDSESIGNQSGGVRQREPLMDGAHPKVVESFKDV"
     gene            18270..18962
                     /locus_tag="NBI57_000496"
     CDS             18270..18962
                     /locus_tag="NBI57_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HCD1821473.1"
                     /translation="MYVDRKPSLYLEDLRHDFKNSLSKFENGDEAFDTLLGFVELDHI
                     YSSALKEISTKLSILDDNFNHIYKHNPIHHMERRVKEMRSLIEKLNRKGLQISAETAK
                     EHILDIAGIRVVCNYLDDIYLIEEMLLKQEDVQLIKRKDYIQHPKENGYRSLHIVVSI
                     PVFLAERVEVLPVEIQIRTIGMDMWASLEHKIRYKNNAETEKYRDLLKECATEITEVE
                     DKLQQIHSEITE"
     gene            19127..19891
                     /locus_tag="NBI57_000497"
     CDS             19127..19891
                     /locus_tag="NBI57_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HCD1821474.1"
                     /translation="MSNYSTGELAKLCNVTTRTIQYYDRKGILKPQGFTEGKRRVYTE
                     QQRQTLELILLLKDLGCALSDIDMLLKGEGTLKTLNTLLTMKQQEINQQVKQQQAVLN
                     KIKNVQYYVNEASTSPITHLKDIEHVMSKSAEMKSIRRNIWISAGIIGIIQYSSIMSS
                     ILMKNKWPFLIALPFMNCYGIGVTFYYQQKVAYLCPNCQHIFSPSLWAVIKAKHTATT
                     RRFECPNCHETHYCIEVPKAHMSTEQLEISHIQHNN"
     gene            20044..20724
                     /locus_tag="NBI57_000498"
     CDS             20044..20724
                     /locus_tag="NBI57_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCD1821475.1"
                     /translation="MMYGYPEKWLEGMTTGEGIAAELRLGIVNGHIAEGTLLTENQMA
                     KQFNVSRSPIRDAFKLLQQNQLIQLERMGAHVLPFGEQEKKEMYDLRLMLESFAFSRV
                     KNQERLPIVKEMKKQLEMMKVAVKFEDAESFTKHDFEFHETLIKASNHQYLNSFWSHL
                     KPVMMALVLTSMRQRMQQNPQDFERIHHNHQVFIDAVEQYDSQILKEAFHLNFDDVGK
                     DIEGFWLN"
     gene            20748..22301
                     /gene="gntK"
                     /locus_tag="NBI57_000499"
     CDS             20748..22301
                     /gene="gntK"
                     /locus_tag="NBI57_000499"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HCD1821476.1"
                     /translation="MKYMIGVDIGTTSTKSVLYDENGAFIMKHQIGYDLHTPNVDVSE
                     ENPDELFDAVLMTIKYIMRESKVNQDDIKFVSFSAQMHSLIAMDQQHQRLTNNITWAD
                     NRAAKYATVINEVHDGNAIYQRTGTPIHPMSPLVKIFWMKHEWQDVFQRTAKFADIKT
                     YIFYHLFDTYIIDYSMASATGMFNLETLDWDVGALELLGISKEMFPELVPTTYVMKGM
                     KERYATLMGLNEDTPFVIGASDGVLSNLGVNSVGKGEVAVTIGTSGAIRTVIDKPRTD
                     YKGRIFCYVLTEDHYVIGGPVNNGGVVLRWLRDELLASEVETAKRLGVDPYDVLTQIA
                     KRVKPGADGLIFHPYLAGERAPLWNANARGSFFGLTLSHKKEHMIRAALEGVLYNLYT
                     VYLALIEVMNETPNMIKATGGFAKSEVWRQMMSDIFDTELVVPESYESSCLGACVLGL
                     KAVGDIEDFSIVSSMVGATNNHTPFEENVAVYQELVSIFINLSRSLTENYEQIADFQR
                     QHMAENKTQ"
     gene            22418..23776
                     /locus_tag="NBI57_000500"
     CDS             22418..23776
                     /locus_tag="NBI57_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate permease"
                     /protein_id="HCD1821477.1"
                     /translation="MFNEIWPLISVVLGIVILLVLIIGFKLNTFISLIITSMITALML
                     GIPLTKIMETIEKGMGSTLGHIALIFGLGAILGKLLADGGGATRIADTLIQKFGQKHV
                     QWAMLVAAFIVGIALFFEVGLVLLIPLVFTVAKRANVSVLKLGLPMVTALSVTHGFLP
                     PHPGPVVIAKELKANVGDVLLYGMIIAIPVTLIAGPIFNKVAQKMIPSAYTREGDISA
                     LGAQKEFTDQEMPGFGMSLLTATLPVILMLVSTITQLVTGHDKPTNLFESIIYMIGTA
                     GTAMLIAVLFAIVTMGLMRKRKMNHIMESVTNAIYPIGMMLLIIGGGGTFKQVLIDGG
                     VGNTIAKMFEGTEMSPILLAWIVAAVLRIALGSATVAAISTTGIVLPLLQSSDVNVAL
                     VVLAIGAGSVILSHVNDAGFWMFKEYFGLTVKETFLTWSLLETIISVSGIIFILFISL
                     FV"
     gene            24159..24261
                     /locus_tag="NBI57_000501"
                     /pseudo
     CDS             24159..24261
                     /locus_tag="NBI57_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501263.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            24245..27361
                     /gene="fnbA"
                     /locus_tag="NBI57_000502"
     CDS             24245..27361
                     /gene="fnbA"
                     /locus_tag="NBI57_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000794614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin-binding protein FnbA"
                     /protein_id="HCD1821478.1"
                     /translation="MKNNLRYGIRKHKLGAASVFLGTMIVVGMGQDKEAAASEQKTTT
                     VEENGNSATDNKTSETQTTATNVNHIEETQSYNATVTEQPSNATQVTTEEAPKAVQAP
                     QTAQPANVETVKEEEKPQVKETTQPQDNSGNQRQVDLTPKKVTQNQGTETQVEVAQPR
                     TASESKPRVTRSADVAEAKEASDVSEVKGTDVTSKVTVESGSIEAPQGNKVEPHAGQR
                     VVLKYKLKFADGLKRGDYFDFTLSNNVNTYGVSTARKVPEIKNGSVVMATGEILGNGN
                     IRYIFTNEIEHKVEVTANLEINLFIDPKTVQSNGEQKITSKLNGEETEKTIPVVYNPG
                     VSNSYTNVNGSIETFNKESNKFTHIAYIKPMNGNQSNTVSVTGTLTEGSNLAGGQPTV
                     KVYEYLGKKDELPQSVYANTSDTNKFKDVTKEMNGKLSVQDNGSYSLNLDKLDKTYVI
                     HYTGEYLQGSDQVNFRTELYGYPERAYKSYYVYGGYRLTWDNGLVLYSNKADGNGKNG
                     QIIQDNDFEYKEDTAKGTMSGQYDAKQIIETEENQDNTPLDIDYHTAIDGEGGYVDGY
                     IETIEETDSSAIDIDYHTAVDSEAGHVGGYTESSEESNPIDFEESTHENSKHHADVVE
                     YEEDTNPGGGQVTTESNLVEFDEESTKGIVTGAVSDHTTIEDTKEYTTESNLIELVDE
                     LPEEHGQAQGPIEEITENNHHISHSGLGTENGHGNYGVIEEIEENSHVDIKSELGYEG
                     GQNSGNQSFEEDTEEDKPKYEQGGNIVDIDFDSVPQIHGQNKGDQSFEEDTEKDKPKY
                     EHGGNIIDIDFDSVPQIHGFNKHNEIIEEDTNKDKPNYQFGGHNSVDFEEDTLPKVSG
                     QNEGQQTIEEDTTPPTPPTPEVPSEPETPMPPTPEVPSEPETPTPPTPEVPSEPETPT
                     PPTPEVPSEPETPTPPTPEVPSEPETPTPPTPEVPAEPGKPVPPAKEEPKKPSKPVEQ
                     GKVVTPVIEINEKVKAVAPTKKAQSKKSELPETGGEESTNKGMLFGGLFSILGLALLR
                     RNKKNNKA"
BASE COUNT         9775 a         4208 c         5169 g         8613 t
ORIGIN      
        1 ctatattttg tccaaatgat aaatatatcg ttatattttt acaatattct gaaatttatg
       61 ttcgcctccg aatgctatat ccagtgtaat gtgttttgca tatatgaaag caatttcaaa
      121 atgtgaatat agtttcattg tggtatgaca aacttcattg cttgtcatga gatggatata
      181 atgaagacga ctaaaagagc aatgaggagg ataatcatgg ttaaggctat tgcggtagat
      241 atggacggga cgtttttaga ttcgaaaaag acatacgata aaccgagatt tgaagcgatt
      301 tttactgaac ttagaaatag agatattaca tttattgctg cgagtggcaa tcaatatgcg
      361 aagttgaagt ctattttcgg ggatagagat atgtatttta tttctgaaaa tggtgcagtt
      421 atttataaag gcaatgagtt atataattat aaaagcttta atcgtcaggt gtttcaacag
      481 gtcgtcgatt acttaaatat gaaccaaagt attgatcaac tcgtcatctg tggtttgaaa
      541 agcgcgtata ttttaaaaca tacttctgaa gcatttaaag aagatacgag attttattat
      601 catcagttaa aagaaattga cagtctacaa caattacctg aggatgatta tgtcaaaata
      661 gcatttaata ttaatcgtga gacgcatcca aatgttgacg aagaagtagc aacgcaattc
      721 agcaatgata ttaaacttgt ctcaagtggg cacgatagca ttgatattat tatgccaaac
      781 atgactaaag ggcaggcatt aaaacgattg ttagataaat gggaaatgtc tccttcagag
      841 ctcatggcct ttggagatgc gaataatgat aaagatatgc tagcgttcgc taagcatagt
      901 tatgtgatgg aaaatagtca tgatgaagaa ttgttcaata ttgcctcggc tgtcgctcca
      961 agtaatgata agcagggtgt tttaacgatt atcgaacaag aagtattgaa caagtaggta
     1021 gcacatcatc aatgaagcgg ctatcatgat actaaataca tgttaaatga aaactttgaa
     1081 tacgaatcat caaacattaa tatatctcaa acaggttagg caataatgcc atagtaaaat
     1141 aagcgcacta caatcagttg aatagatgat aaaataaagc agattttaat caaaccgatt
     1201 ttgatacaga taaacaaaat aaaaagcaaa ggcacatggt gcgcctttgc ttttaaatat
     1261 atattaattt aaacgtgttt cacatgtacc agtgttaatg acagataatg ctgcgtttaa
     1321 accaccttca acaaggtttt gtactgcttc atcagagaag aaagcaatat gtggtgttac
     1381 taaaattctt tcatgttcga ttaactctaa taatgtttta tcgtcaatgt ctttattagt
     1441 ccagtcattt gtgaagtatg cagcttcatt ttcataagta tcaatcgcag cacctaacaa
     1501 agtaccatcg ttcactgcag cgattaaatc aggtgtattg atgactgcac cacgtgctgc
     1561 gttaactaag attgcacctt ttttcacatg atcaaacatt gctttatcga ataaatgata
     1621 gctttctttg ttcgctggaa catgtaaaga aataatatcg gcatctttaa tagcttcttt
     1681 aacactatct ttataagtta aaaagtctaa atctttatta ggataagcgt cataagctgt
     1741 aattgtagca ccaaatcctg catatatttt agctgtagca gcaccgatac gacccgtacc
     1801 gataattgca acagtcatat ttttaactgg tttagacatg atttctgctt gccaagtaaa
     1861 atcatgtgtt tgtacacggc gttcaatatc tgggaagcga cgcactaatt gtagggcgat
     1921 agaaacagaa tactctgcaa ttgtttcagg tgaataacta ggaacgttag atatcacaat
     1981 gttgtgtttt ttagctaaat ctaaatcata catatcaaat ccagcagtac gttgtgcaat
     2041 ttgtttaata ccgtaagatt ctaatttagg ataaacgtca ttttctaact taccaaattg
     2101 cattgtagtt acgccatcgt aatcttttaa ttgatcgact gtagcacttg ataatagctc
     2161 tttagaagta gttacttcga cattattctt tttcccccaa tttaatgcca tctctttctc
     2221 ataatcacgc gtaccaaaga acataatttt tgtcattatt aaaaacctcg cttttaaaag
     2281 attgaaaagt aaatgagtga aattaaagat tatgtacatt aaaatcacgc cacaatttaa
     2341 ttgtgaaaaa tatcacaaat atattataac actaaatttt ccaaaattca aaagtgtgtt
     2401 ttattgcaga aaacttataa catgtgcaca agttatagcg aattgcaaac ggattacttt
     2461 agtcttttta aaacatgaag tataatttgt atagtaataa atataaaaat tggaggctat
     2521 gttcaatgag caatatgaat caaacaatta tggatgcatt tcatttcaga catgcgacta
     2581 agcaattcga tccacaaaag aaagtttcga aagaagattt tgaaacaata ttagagtcag
     2641 gtagattgtc tccaagttct cttgggttag aaccttggaa gtttgtcgtg attcaagatc
     2701 aagcgttacg tgatgaatta aaagcgcaca gttggggcgc agcaaaacaa ttagatacag
     2761 cgagccattt tgtgctaatt tttgcgcgta aaaatgtaac gtcaagatca ccgtatgtac
     2821 aacatatgtt aagagatatt aaaaaatatg aggcacaaac gatcccagct gttgaacaaa
     2881 aattcgatgc attccaagca gatttccata tttctgataa tgatcaagcc ttgtatgact
     2941 ggtcaagtaa acaaacgtat atcgcattag gcaatatgat gacgacagcc gcattgttag
     3001 gtattgattc atgtccgatg gaaggtttta gtctggatac agtgacagac attttagcaa
     3061 ataaaggtat cttagatact gagcaatttg gtttatcagt gatggttgca tttggctaca
     3121 gacaacaaga tccaccgaaa aataaaacac gccaagcata tgaagatgtt attgaatggg
     3181 ttggaccaaa agaataaata gaatacagta tgtctaaata tataaaatta aaaagttagc
     3241 aataaaaaaa gcctgcgatt acataaatga atcgcaggtt tttgcgtgaa aaaattgtat
     3301 taataaagta tggatgatta tttttctgga acaaggtcag tatttgaatg aactgtgatg
     3361 tcaaaccctt ctggtgccgt aaatgtatgt gttgaggcgt cgggttgata aatatcaaca
     3421 tgtgttaatc cataactttg tgcattgttt tgtcttgctt gattggattg ccaagtatta
     3481 gcagcaatat gatggtgata atgattcgtt gacataaata gcgcacgtgg aaaatcagac
     3541 acatgttgga atcctaattg ttcaatgtaa cattgatatg ctgcgtctaa atcatgtgtt
     3601 tttaaatgta agtgtccaat catgcctttt gctggcattc cttgccaacc ttcatcagta
     3661 cgatgtgtta ataaggtttg gctatcaact tctaaagtat ccattttaac tttgccattt
     3721 tgccattccc atgaagatga aggtctatcg cgatagactt caataccatt accttcgggg
     3781 tcgttgaaat ataaagcttc acttactaaa tgatcaccag cgccaatgcc catatttttt
     3841 tgtgccacga aatataagaa gttagctaga tcctcagtag ttggtaataa aaatgctata
     3901 tgaaaaagtc ctgcttcacg tggggaagtc tgacggccgt cttctaataa atgtaacgtt
     3961 agagtatggc caccagtccc aacagataat acggttgtgt tatcgtcaga acttttaacg
     4021 gatagtccta aaatgttttt gtaaaatgtt gtcattaagt ctaagtctct tacgttcagt
     4081 tcaatgtttg tcacttgtgt tgctgtttta tcgtgaaatg ccattatgca tcgcctcgtt
     4141 ttctattttt ctataagtta gtataaaaag tataccagaa aagaaaatga attgatagca
     4201 taaagtttga aatgcaaaat aactagtcgt tttgcaattt tatattgatg cgaacaaaaa
     4261 agcgatggta cagttgcacc atcgcaaaat ttatttaacc aagatataca tcttgatatg
     4321 aatcttcttt ttctaacata tgtttggcaa atgaacatga ggcaataatt tttaaatgat
     4381 tttctcgagc gtgttcaaca actgctttaa ctagtttttt gccaacacct tgaccaccaa
     4441 gttcatcaga tacgcctgta tgatcaatgt taatttcatt attatccaca aaacggtatg
     4501 tgatttcagc taaagcatta ttttcatcat caccaatata gaatttgttc tcgccttgtt
     4561 tgatttcaag gttactcata catatcaact cctatcatgt ttgattataa tatttcccta
     4621 ttctatttta acttaaacga agtaaaaggt gcatgacagt catgtgacga cattgccgca
     4681 tttatgtagt cgtttttatt aagcgcaatt tgaaataaag atgaaaacac gtatcttgac
     4741 attaaatcca ttcagctata taatttatct cgaaatcgaa ataaattaaa aagttggtga
     4801 tgatatggat cgaacgaaac aatcgctcaa tgtctttgtc ggaatgaata gggcgttaga
     4861 cacattagag caaattacaa aagaagacgt aaagcgatat ggcttaaata ttactgaatt
     4921 tgcagtgctc gagttgcttt ataataaagg tccgcaacca attcaacgta ttagagaccg
     4981 cgtattaatt gcaagtagca gcatttcata tgttgtaagt caattagagg acaaaggttg
     5041 gattacacgt gaaaaggata aagatgataa acgtgtatat atggcttgtt taactgaaaa
     5101 aggtcaaagt caaatggcag atattttccc taagcatgct gagacattaa caaaagcgtt
     5161 tgatgtgtta acaaaggatg aattaacaat cttacaacaa gcgtttaaga aactaagtgc
     5221 acaatctaca gaagtgtaag gcgtgcacta aaaatttaca ttaaaatatc tcgatttcga
     5281 gataaataca ctaaaaatat aaagagggta tataaaatga taaataatca tgaattacta
     5341 ggtattcacc atgtcacagc aatgacagat gatgcagaac gtaattataa attttttaca
     5401 gaagtattag gcatgcgttt agttaaaaag acagtcaatc aagatgatat ttatacgtat
     5461 catacatttt ttgcagatga tgtaggttca gcaggtacag atatgacatt ctttgatttt
     5521 ccaaatatta caaaagggca ggcaggaaca aattccatta caagaccgtc ttttagagtg
     5581 cctaacgatg acgcattaat gtattatgaa cagcgctttg atgaatttgg tgttaaacac
     5641 gaaggtattc aagaattatt tggtaaaaaa gtgttgccat ttgaagaagt cgatggccaa
     5701 gtgtatcaat taatttcaga tgagttaaat gatggggtag cacctggtgt accttggaag
     5761 aatggaccgg ttccagtaga taaagcgatt tatggattag gacccattga aattaaagta
     5821 agttattttg acgactttaa aaatatttta gagactgttt acggtatgac aactattgcg
     5881 catgaagata atgtcgcatt acttgaagtt ggcgaaggcg gcaatggtgg ccaggtaatc
     5941 ttaataaaag atgataaagg gccagcagca cgtcaaggtt atggtgaggt acatcatgtg
     6001 tcatttcgtg tgaaagatca tgatgcaata gaagcgtggg caacgaaata taaagaggta
     6061 ggtattaaca actcaggcat cgttaatcgt ttctattttg aagcattata tgcacgtgtg
     6121 gggcatattt taatagaaat ttcaacagat ggaccaggat ttatggaaga tgaaccgtat
     6181 gaaacattag gtgaaggttt atcattgcca ccatttttag aaaataaaag agagtatata
     6241 gaatcagaaa taagaccatt taatactaag cgttaacatg attaattgga atgaggagga
     6301 tttgtgatgg aacatatttt tcgtgaagga caaaaaggtg caccaacgct aatattgttg
     6361 catggtacag gtggcgatga attcgattta ttgccattag gcgaagcatt gaatgaaaat
     6421 tatcacttgt taagtattag aggacaagtt tcagaaaatg ggatgaaccg gtatttcaaa
     6481 cgtcttggtg aaggtgttta tgatgaagaa gatttggaat ttcgtggaca agaattgtta
     6541 gcgttcatta aagaagctgc tgaacgttat gaatttgata ttgaaaaagc agtacttgtt
     6601 ggattttcaa atggatcaaa tatagcgatt aacttaatgt tgcgttcgga agcaccattt
     6661 aaaaaagcat tgttatatgc accgttatac ccagttgaag taacgtcaac aaaggattta
     6721 tcagatgtaa gtgtgttgct ttctatgggg aaacatgatc caattgtgcc attagctgca
     6781 agtgaacaag tcattaactt gtttaagaca cgtggggcag tagtcgaaga agtttgggtg
     6841 aatggtcatg aaattacaga aactggatta acggctggtc aacaaatact tgggaaataa
     6901 cagttctatt aagaagcgga cagatggaaa agatttttac ttttcatctg cccgcttttt
     6961 tgattttgaa gtgctgtaat aaattttaca atagtataga tgttttaatc gatatgagat
     7021 ttgccggtaa tacgcttaat taaaccttta tagagtacag gtatgagtaa gatgaaaccg
     7081 aacaatccca taatagggaa tacttttcca attaatgaaa tgaaaccgat aaatgtacta
     7141 atataagtga tgacagccat tgtaataata atgatgaagt aacgtctgct gaatggaacg
     7201 ctgaaacgtg acgcaaatgc atacattaat ccaacaacag tattgtagat gacaagtatc
     7261 ataatgacag acataataat accaattgac ggagacattt gtgtcgctaa ttttaatgta
     7321 ggtaaatcga cgtgtttaat tttatcgaat tgagaaatta aacctaggtt aatcatcatg
     7381 agtaaaaatg taatgattaa accgccaatc aagcccccgt ataacgttga gtcacgatat
     7441 ttaactttgc tacccatcac tgataagaag ctgaaggcag cagcaatttg caagcttgca
     7501 tagttaatcg catcaaacca ccatccaggt gataatgatt tctgcttatg aatctgagca
     7561 tcattattag cggcagtaaa atcaagatga ctcgttgtga aatagtagac cgcaatcata
     7621 atgacaattg caattaaaaa tggagtaact ccaccaagca cagcaattaa acgatcgaat
     7681 tttagaaaca gtgttgctaa aataaaggcg actaatatga gtgcgctcag ccaatacggt
     7741 aagttgaaac tttgatgaat ggttgacgca ccacctgcag tcataataat agctaaagac
     7801 aacataaaca ttgttaaaat aatatcaaaa cctcttgcaa tagaggggta taagaaatag
     7861 ttaattgaat cagaatgatt tctggacttt agatgatgac ctgtatgcat gacaaccatt
     7921 ccacctaaag taatcaatag tcctgttaca ataatgcctg aaatgctata tgcgccatga
     7981 cttgtgaaaa actggaaaat ttcttgacca gtagcaaagc cggcaccaac gacaacacca
     8041 acaaaggcaa atgccacaat aatggactct tttaagatac gcatgattta aaaatgtccc
     8101 ttcgtaattt taagtaatat agaaaatgta acatacatgt taatgaaaaa tatagtacta
     8161 atatagtatt ttgttaaatt gaagtagaag cgagggcgtc ggtcatttca ttaatttatt
     8221 agttgatttt gcattttttt gctgtaaagt tgttataata cagttaacag gaattagcat
     8281 agatacacca atcccctcac tactcgcaat agtgagggga tttttttcgg tgtagctagg
     8341 tcgcctattt atcatcgcgt ttgcgtagcc aatgcgtaaa ccgcatcaat acaacagtat
     8401 acaatacatg cataaaatgt cgtctttagt ctaagtaacg atcatgcatt aacattttca
     8461 aaatatctat ttgagcttga agctctttac caatattggt atcacgaatc ttcttacgtt
     8521 gtaattcttt gtctacaacg cgctttatag aaagttcatc gataccttcg gaaagtattt
     8581 tttctttagc gttaaattgt tggtgtgcaa cgagttgcat tccgaatgaa ttatacaaca
     8641 atgtatagcc tgcaatgcca gttgttgact gataagcttt tgaaaaacca ccatcaatga
     8701 caagcatctt tccatcagcc ttgataggat cttcgccatt gatttctttc actggtgtgt
     8761 gaccattaat aatgcgtcct tcatctggat ttaatccgaa atcactgagc attttacgaa
     8821 ccatattcac atcttcacgt aaatgatagt atggattttt ttcttcttta tgagaagctt
     8881 tatctgcaat aaaatatcgc tcaaacgtag tcatcgcgcg tttaccaaat aatgacgaat
     8941 atttcccagt ccataaatac caaactaaat ccgtcgataa gtcatcagta ttttcttttt
     9001 catcaaatga tttacggaca tgatactcaa acacatctaa taattcttgg ccgctgtaag
     9061 tatgaccatc aatttcaaat gattccatct caccattttc atcaactgga atacaaccat
     9121 gaatgagtaa attaccatta tatggtaagt aaagcgaacc tttacgcatc aagaaagaca
     9181 tatgacgacg taatttttca gattgttgga atgacaataa tagtttattc atgacttctt
     9241 cttcttcagg tagtaattct gctggattat tacgattgat agtttggaaa catgtgtctt
     9301 tcaacgggta tgtattacca taaactgtaa tttcatttgt atcataatta accttttcaa
     9361 gcacaagacg ttcttccatt tcgaaatttg gacgacgttt aataattggt atttctaact
     9421 tgaattgaat catcgcaata gcttgatgaa ttttagtaat ttgactttct tcacgttgag
     9481 ttaaacgttc gtgtttgtca ggtctttttt taggcttaaa agcaggatta tctgcgtcat
     9541 agtatttttc agctaaagta agcagtggtc ttaaattaat gccataagcg tcttcgataa
     9601 tatctaaatt atcatagcgt gcacaaattc gaagtaagtt tgctaagcat acttttgacc
     9661 cagcataggc tccaacccaa agcacatcat gattacccca ttgaatatct agggaatgat
     9721 aattaatcag tgtatccata attttatctg gttgtggtcc acgatcataa atatcaccga
     9781 caacgtgtaa atgatcgacg actaagcgtt gtacggaata agcgagtcca ataattaaat
     9841 catctgcctg tttaagttca attacttggt taacaagtgt ttcgtaatat gattttttat
     9901 tttgatattc attactttta tacagtagtt cctcaataat ataaacgtat tgttttggca
     9961 atgctttacg cagttttgaa cgcgtatatt ttgaagaaca atatttaatc aactcaatta
    10021 aatgttcgat tgttgtgata taccagacat taagttgacc gcaactttgg aaatcacttt
    10081 taatcaattt taatttgtct tctggatagt agacaagagc agttaaatca ttaagctcct
    10141 tagttgaaag tctctctttg aaaatatcat tgattttcgc tcgcacattc ccagaaccgt
    10201 tgcgtaatac gtgttggaaa gcttcgtatt caccatgtaa atcgctgacg aaatgttccg
    10261 tacctttagg taattctaaa attgattcta aattgataat ttcagttgca agtttttctg
    10321 gagtatcaaa attttgggat agtaaatcta aatacttctt ttttaattct ttttcagtaa
    10381 tttgagtcat tacgtgtcac tcctgtagta tatttcaaac gttagtttat aaaataaatg
    10441 cgcttacaat gattatcaag aaaacgttaa catttttcaa taagtatgct tggattgata
    10501 aaaatagagg gcaggatttt ggtattagga gtcgttgcag tttagttaag taaaagtggg
    10561 aaagtgatat atttatttta aaaatgagag gaggaatttg ttatattcac tagtttccta
    10621 aaaaattacg atatgtaaaa tgtggaattg ccaaaatttg gataaaaaaa tccccgcacc
    10681 attatttggg tgaagggatt tgaaaatgat tttgaattac gatggaatat gtgtgtattt
    10741 attcatctaa tactttaagt tactaaatat ttttcttctt gaaaataaat actacaagta
    10801 ttaagaaaat agaaatataa gcaatattac caataaacaa ttcatgaagt tctaatttcg
    10861 tcgacttact aaagcctttt tcaactgttt gaagcataat attcatcata ttaataggat
    10921 tccactttag ccagtctatt ttttctaaaa gtgccgtttg aataactgct aaaatagaac
    10981 ttgcaaaata aaaaacaata cctacagcaa tggctacacc cgttgaatta gttgcagatg
    11041 ataatagcaa cgttaagctt aaaactaacc aaacgccaac aaatgtacct aaactaacta
    11101 ataataattg attcaataaa gataattgat taccgctact ttcaaatata tttaaatcat
    11161 taaagaataa agacccaata acaattgaag caataattgt aataacaaaa taaattaaag
    11221 aaataataaa taatgtgatg attttgctaa caatcatagt tgttcttgaa tattcacgat
    11281 agagtaaatt tttaatcgta ccgtaatgaa attccattga aatgattgta cttgcttgaa
    11341 taattaataa aaatgcaaac catgaaaaac cattataagc agatgtgaaa gattcctgtg
    11401 gcgtaaaaat ttcattgtat tttgtagcga tataaccttg agcaaccatt agtagaatga
    11461 agacaatagg tgcgataaaa gttgatttct ttttaaataa tttgaaacat tcttgtttaa
    11521 ttaaagttcc cattttatgc ttggtcctcc ctttctatga tttctagtaa tacattttct
    11581 aaatcattat ctttaaattg tttaagttct tcttccgatg tttctgtaac aattttacct
    11641 ttgttaataa taaggataga gtttgtgatt ttaactaact cacttaaaat atgactcgaa
    11701 attaagaaag taacaccttc ttgcgacttt tggacaatca attcacgtac atctcgcaca
    11761 gcttttggat ctaagccgtt cattggttca tctaagataa tgaattgagg ttcatttaaa
    11821 aatgctatag caattcctaa tttttgtttc ataccaagag aatacgtttt agctttttta
    11881 tgaatgtatt catccatatg aagttgtgag acaattttat cgatatcttg agtgtttttt
    11941 gattcattca ataacttcaa gttttcatat ccagacataa aaggatatat tcctggattt
    12001 tcaatcaatg caccgatatt gcttttgctg tctttgctgt taataacatt aaaatttccg
    12061 ctttggaaac tagagtaacc taatataact ttcattaaag ttgttttacc tgcaccattc
    12121 gctccaacga gaccaactat ttgtccgcgt ttcagcttaa atgatacatc ttcgagaatc
    12181 gttttgttgc ctatcttctt tgttaaatgt tctattgtta aaacacccat atcactatct
    12241 cctttgctat taaataatct gaatcttaag attttatata taaaattaat tttatcacat
    12301 agcaagtgtt ataaaaatat ggatttaaaa aactttaata atataaaata taaatatgaa
    12361 cacgagtatt ttttgtagcg tgcaaatggt attgctacat ttattaaaat gcgcaatttg
    12421 ttatgagtaa cttttttgga tagtaggcac attaaagtgt cgaaaacaat ttgtgtggag
    12481 gacagggtaa attctgttta tttaagatga tagtttgtat tatagatgga tttgtgagat
    12541 aagtttgcgg aatgtataca tactgaggga actgaacttt gatgggaaac ttcattatat
    12601 ctcttaacgt aaaggatttt aggtgacttt cagctaaagt atgccaaata aacttaaaaa
    12661 agcagtgaat catttcaata gatggaatga ttttactgct ttttgtgtat agaaatttaa
    12721 aattcatgcc tttatactaa caacaatatt attttactct cttacgtttg ccaatgtaac
    12781 gtatagcaaa gtaaatgact gcgataatga tgacaacata cataatacgt gaatatgtat
    12841 gaagaccagt catcaacata ccaaagctat cgctcaacgt gcgtcctagt aaaattaaac
    12901 cgaaattcca aattgttgta cctattaatg aaataacggt aaatgtcaca acattcatgc
    12961 ggtttacacc ggctggaatg gtaatcaata cacgtaatac aggtatgaaa cgacagataa
    13021 atacagccca tacgccatac tttttaaacc aatcatttgc tcgctttaaa tccttacttt
    13081 tcaatttaat ccacttaccg tgtcgatcaa taaaacgata tagacgttct tctgaaatca
    13141 aacggcagat ataatataaa attaacagcc ctataaacga tgcgatggtt gcaataataa
    13201 ataaggttaa aattgataaa tgtgatttaa cagacataag gccagcaaat gtcagaataa
    13261 tttccgatgg tacgataggt aaaacatttt ctaataaaat taatataaaa atggctgcat
    13321 acccaaaacg actaataaat tcagtgataa tttgttccat tattaaaaat aagctccttt
    13381 aattttcagc aattgcagac aaatagaaac tggataaatt caaatgaata atgaatgatg
    13441 tcgtatgcga tgttgaaaca agcatttgtc ttaaaatgta ttataagcaa taaatcaaaa
    13501 aattgcttgc tataagtacg aaaagtcaac aaatgatcat ctaaataaca ggcgctgcac
    13561 aatgaatgag caatgtagtt ttatgcaatt taaaatgaat gtcgaatatg taagaaagcc
    13621 tgcaatatac gttttaaaac actaggattg cagtattttt aattaacaaa tgataggcta
    13681 ctatgtaact aaaagttcct ttatatcgtt ttaatagcaa aagttaagcg gaatttaata
    13741 aaatttttat agattctatt gtaaacgctt tccatgtctg ataatattat aaaaaataac
    13801 tttggtacaa ttttgtgaca aagtaggggt ggacatgcca gatgtccgaa aattttaacg
    13861 tttcagggta agttttaaaa aattaattag ggaagtagga atagttatga aggaaaaacg
    13921 aactaacgta aggtggatgt tcgcgcttgc gttcttcttt attggggtta ttgcgtatat
    13981 ggatagagca aacatttcat atatcgctaa acaaatgatg gatgatttag ggatgaccaa
    14041 accacaattt ggtttattgg catcattctt ctctctaggt tatgcattaa tgcaagtacc
    14101 atcggggatg ttggctgaaa aattcggtcc acgtaagatg attacaattg cattagtttg
    14161 gtggagtgca tttacaatct taacgggtat gattaagaac cacggtttaa tttatttagt
    14221 gagattctta tttggtgtgg gtgaggcgcc aatgtatcct tctaatgctg tgtttaactc
    14281 attttggttc tctaaaaatg aaaaaggtag agcatcaagt gcattattag caggatcata
    14341 tttcggacct gtattagcac caatagttac aattgctatt gttaacgcat ttaactggca
    14401 agcagtattt tacatttttg gtgcagtagg tattttaatg gctgtattat gggcgattat
    14461 tgccaaagac ttacctgagc aacatagaat ggttaatgaa gcggagaaac gtttcattat
    14521 ggaaaatcgt gatatcgtag ctactgaaaa gtcatcacca ccatggaata atttctttaa
    14581 acgttttagc ttctatgcaa ttgcaattca atactttgtt gtacaattta tcattacatt
    14641 attcttaatt tggttaccga cgtatttaac agaagtattc cacgttaact ttaaagaaat
    14701 gagcattagt tcattacctt ggttattaat gttcttctta atcttatcag caggtgcaat
    14761 ttctgaccgt gtattaggat taggtcgttc aaaattcgta gctagaggtg taattgcaat
    14821 cgcaggattt attgtgtttg cagtttcaat tatctttgct gtacgtacag gaaatttata
    14881 tgtaagtatt ttctggttat cactaggtct tggtggtatc ggtatttcaa tgggtatgag
    14941 ttgggctgca gcaactgact taggacgtaa cttctctggt acagtatcag ggtggatgaa
    15001 cttatggggt aatataggtg cattaatcag tccgctatta gcaggtctat tcgtagaaca
    15061 tttaggttgg acaatgacat tccaattgtt aatcgttcca gcagtaatcg ctgtgattat
    15121 gtggttctat gtgaaaccag atcaaccttt aattgttagt gatgataaag caatagaaaa
    15181 ataatttaaa caagcagtaa gctttcacat tgttggggct tattgctttt ttacgttgaa
    15241 attgaaactt tttaaaacag atatggttta agatgaacgg gaagttatta aaatgatata
    15301 tgtaaagaaa taaagtttta aaacattagt caggtaacgc ttgtaaaagt acatataaat
    15361 tttaactagc ccaaaggtgg gcgaccaaag gttcaacgat gttaaataac attagaaatt
    15421 aattttaatt ggactttaaa agtttttaaa tttagataat tgagcataag gtgttataat
    15481 cacatatgtt gcgtaattaa aatttatagc aacaaattca ttttaactat gctaataaaa
    15541 agattatgga aatattttga caaggaaagg agaagtcgaa atgacatctt tttgacatca
    15601 cttataaaaa tcaatcgact taacttagac ttttataaag gtgtaagaca gggactgtta
    15661 atgattattc ctgcaataat cggttactta tgtggtaatt tccaatttgg attattagtt
    15721 gcaaccggaa cactagccca tatttatgtt tttaaaggtc cgtcgcgatc taagctgcga
    15781 actgtaataa tttgtaattt agcgtttgca atatgtatga tgcttggtac gctaacagcc
    15841 aaaacgccac tcgtttttgg aatgacatta ttaattgtta cggttatacc attttatata
    15901 tttactgcct taaaaatagc tggaccgtca tcgacattct tcattgtgac attcagtcta
    15961 cccattaact tacctatagc tcccgaagaa gcattatata gaggctttgc gattttagta
    16021 ggcggtatac ttgccactat gatggtgtta atcacgatcg tattttctaa aaacaaagct
    16081 gaagaacaag caattcaaaa tgattttaaa ctcatatcta agttgttaca cacttataat
    16141 gataaagctg cttttttaca agtagcaaaa acagcggtgg atagttttaa agcatctgat
    16201 aaattattaa tcacttctac ttcaagtaac gataaattaa gtagacgttt ccaaaaatta
    16261 ttattattac acacatctgc ccaagggatt tattctgaac tgttagagtt gaacgctaaa
    16321 caaattcgac cattgccaga tgagttaatt gaaatgatgg atcatatcat tgcacaacta
    16381 gataatagtg atgaaaatgt aagatattgg cgaaaagaag tgacagtaac agaggaattt
    16441 caaaatttat tcaaccatat attgaaaatt gatgaaatgg tgcatgcaaa tgaagcgcgt
    16501 attgcgtatg aagcagacat gcgaaaacct ttatatagta aacgcattta tcaaaattta
    16561 acattagact ctattgtttt tagaaataca ttgagatata cagcgattat gatgatagcg
    16621 atatttattg cgttaatgtt tgattttgaa aaagcatact ggataccgtt atctgcacat
    16681 acaatattac taggaacatc aactatacat gcaatcgaga gaggtatggc acgaggttta
    16741 ggtactattt taggtgtgtt agtactttca gtcatattgt tgttttcaat accaacacct
    16801 gttgcagtaa ttttaatggg cattgcagca ttgtttactg aagcattggt gggagcaaat
    16861 tatgcgattg cagtagtttt tattacaata caagttattt taatgaatgg attagcatca
    16921 cagaatttaa caattaacat tgcgtttcca agagttattg acgttgcaat aggtattgtg
    16981 attgcaatca taggtttatt tgtccttgga caacgtaccg catccgtatt gcttcctaat
    17041 gtaatggctg aagttgttcg taaagaagca acgctcttcc attatttgtt ttctgaaaac
    17101 caatataaag ataatgtgta tcagaaaaac acggccatga acctatctgt gaaattaaat
    17161 aacatgacgc aagtttataa tgcagctaat ggtgaattat ttagtgataa gacattgatt
    17221 caaaattatt atcctagctt attcgcatta gaagaaatta gctttatgtt aaatagagcc
    17281 atggctaatg aagatagact tacaataaat gaacaattaa tgggcgagta tttagcaaca
    17341 tttgaaaaca tcgctaagca tttagaatta aatacagaat tagaaataaa gatattacca
    17401 gatttaccac agtataatta tattcaatca gcaatgatga atattcaaca caatggtttt
    17461 cgtgagagag acaagaacgt ttaaaataaa atatttatca ggtcagacta acgtttgtct
    17521 gaagttatta tgacgggagt ctaaaaaatt gtgtttaata tgtttcattt tataattatg
    17581 gtggtatata acatgaatta taacatattg gaggttaagg aaatgccctt tacaatgaaa
    17641 ggttaccccg aaaaatggaa agaatttgaa gtaattaaac ggcaaaaagc gatagaaatt
    17701 gcgaatgcaa tgttaaaaga aggatatctt gaaaaagatg ttgttcctat tgcggcgaaa
    17761 aaagcgaaag attggtatcg agcgttatcg aaagaagaaa tcaaagcatt agaagaagag
    17821 aacttcgtac aattccatac agcaacaatg gaaaaagcag aggtggatag tgaatatgca
    17881 acaactgata atgaagaaca tattcaagaa caatcaaatc aagataaatc gtactttgat
    17941 agcgaatcaa ttggcaacca aagtggtggc gttcgacaaa gagaaccgct tatggatggt
    18001 gcacatccaa aagttgtgga atcatttaaa gacgtataac atctctatca attaagcact
    18061 tgtacctata taacggtaac aggtgttttt ttgcgttaaa tcattttaaa attacttcct
    18121 gtaattgaag gttttgatac ataattcgga ttgtttgagt ttctttgtgc tgtttcagta
    18181 tcagcccaaa gttcgatagt gtattagtat tatcttaata aaatgttagg tacaataaag
    18241 atgattatat atcggaggtt agtataaaaa tgtatgtaga tcgaaaacca tcactatatt
    18301 tagaggattt gcgacatgat tttaaaaata gtttaagtaa atttgaaaat ggtgatgaag
    18361 catttgatac gttattaggt ttcgtagagt tagatcatat ttattcgtca gcactaaagg
    18421 aaataagcac taaactgagt attttagatg acaatttcaa tcacatttat aaacacaatc
    18481 ctatacatca tatggagcga cgtgtgaaag aaatgcgtag tttaatagaa aagcttaatc
    18541 gtaaaggatt acagattagt gcagaaactg ccaaagaaca catactggat attgcgggaa
    18601 ttcgcgtagt atgtaattac ttagatgata tttatttgat tgaagagatg ttgcttaaac
    18661 aagaagacgt acaattgata aaacgtaaag attatattca gcaccctaaa gaaaatggtt
    18721 accgcagttt acatatcgtt gtatccattc cagtcttttt agcagaacgc gttgaggtat
    18781 tgcctgttga aattcaaatt agaacgatag gtatggatat gtgggcaagt ttagaacata
    18841 aaatacgtta taaaaacaat gcagagacgg aaaagtatcg agatttactg aaagaatgtg
    18901 cgacagagat tactgaagtt gaagataaat tacaacaaat tcattctgaa ataacagagt
    18961 agacgtttag taggagtgtt gcttaatttg agtgattttg taaatcaaca tggtacgaaa
    19021 tcataaaaat aagacgccaa gtataataaa tttcttaaaa atatggaaat tgtacttgct
    19081 cgtgacgtcg ggtcatcaac tatggtgatt gtaggaggtg gaatctatgt ctaactattc
    19141 gactggagaa ctcgcaaaat tatgcaatgt gacaacacga acgattcaat attatgatcg
    19201 caaaggtatt ttgaaaccac aaggatttac agaaggaaag cgtcgtgtgt atacagaaca
    19261 acagcgacaa acattagagt taatcttatt gcttaaagat ttaggttgtg cgttaagcga
    19321 tatagatatg ttgctaaaag gtgaaggtac tttgaagaca ctcaatacct tactaaccat
    19381 gaaacaacaa gaaattaacc aacaagtcaa acagcaacaa gcggtattaa acaaaattaa
    19441 aaatgttcaa tattacgtaa atgaagcgtc gacgtctcca atcacacact taaaagacat
    19501 agagcatgtc atgagtaaat cagctgaaat gaaaagtatt cgtcgtaaca tttggattag
    19561 tgctggtatt ataggaataa ttcaatattc tagcattatg agttcaatct tgatgaaaaa
    19621 caaatggccg tttttaattg ctttaccatt tatgaattgt tacggcattg gtgttacttt
    19681 ttattaccaa caaaaggttg cctatttatg tcctaactgc cagcatatat tctcaccatc
    19741 tttgtgggca gttatcaaag cgaaacatac agcgacaaca cgtcgattcg aatgtccgaa
    19801 ctgtcatgaa acgcattatt gcattgaagt acctaaagcg catatgagta cagaacaatt
    19861 agaaatatcc cacatacaac ataacaatta aatcattcgt gtgatagtga tcattccctt
    19921 taaatttaca tcgaagaggg gtggtcacta ttttagttcg cataaagaaa gcgcttgcaa
    19981 acacttaaga actatattaa catacttgta tacaagtatg tcatgtaatg aaggagtgct
    20041 ttgatgatgt atggatatcc agagaaatgg ttggaaggta tgacaactgg agaaggtatc
    20101 gcggcagaat tacgcttagg tattgtgaat ggtcacatag ctgaaggtac gttactcact
    20161 gaaaatcaaa tggcaaagca atttaatgtg agtcgttcgc caattcgaga tgcatttaaa
    20221 ttattgcaac aaaatcaact catccaatta gaaagaatgg gtgcacatgt gttgccgttt
    20281 ggggaacaag aaaagaaaga aatgtacgat ttgcgactga tgctagagtc atttgcattt
    20341 tcaagagtta aaaatcaaga gagactacct atcgtaaaag aaatgaagaa acaacttgaa
    20401 atgatgaaag tggcagtaaa atttgaagat gcagaatcat ttacgaagca tgactttgaa
    20461 tttcatgaaa cattaatcaa agcatctaac caccaatatt taaactcatt ttggagtcat
    20521 ttaaaaccag taatgatggc actcgtttta acatccatgc gacaacgtat gcaacagaac
    20581 ccgcaagatt ttgaacgcat acatcataat catcaagtgt ttattgatgc agtggaacaa
    20641 tacgacagtc aaattttgaa ggaagcgttt catttaaatt ttgacgatgt tggtaaagat
    20701 attgaaggat tttggttgaa ttaaaatgaa agaaagaagg atttatcatg aaatatatga
    20761 ttggtgtcga cattggaacg acgagtacca agtcagtctt atatgatgaa aatggagctt
    20821 ttatcatgaa acatcaaatc ggctatgatt tacacacacc aaacgttgat gtctcagaag
    20881 aaaacccaga tgaattattt gatgcggtat taatgacaat taaatacata atgagggaat
    20941 cgaaagttaa tcaagatgat attaaatttg tgtcatttag tgcgcaaatg catagcttga
    21001 ttgcgatgga tcagcaacat caaagattaa caaataatat tacttgggca gataaccgcg
    21061 ctgcaaaata tgcaacagta ataaatgaag tgcatgatgg caatgcgatt tatcagcgaa
    21121 caggtacgcc tattcatcct atgtcgcctt tagtgaaaat tttttggatg aaacatgaat
    21181 ggcaagatgt atttcaacgt actgctaagt ttgcagatat taaaacatac attttctatc
    21241 atttatttga tacatatatc attgattatt caatggcttc cgcaacaggg atgtttaatt
    21301 tagaaacatt agattgggat gttggggcat tagaattgct tggtatttcc aaggaaatgt
    21361 tcccggaatt agtgccaaca acgtacgtaa tgaaaggcat gaaagaacgt tatgcaacat
    21421 taatggggct taatgaagat acaccgtttg ttattggtgc gagtgatggg gtcctttcta
    21481 atttgggtgt caatagtgtt ggtaaaggag aagttgctgt cacaatcggt acatctggcg
    21541 cgattcgtac tgtgatagat aaaccacgta ctgattacaa aggtagaata ttttgttatg
    21601 tcttaacaga ggaccactac gtcatcggag gtcctgtaaa taatggtggt gtcgtattga
    21661 gatggttgcg cgacgagttg ctagcgagtg aagtcgaaac tgcgaaacgt ctcggtgttg
    21721 atccttatga tgtcttaact caaattgcaa agcgtgttaa accaggtgca gatggtttaa
    21781 tattccatcc ctatttagct ggagaacgtg cgccgctttg gaacgcaaat gcaagaggtt
    21841 cattcttcgg tttaacttta tctcataaaa aagaacatat gattcgcgct gcattagaag
    21901 gtgttcttta caatttgtat accgtctacc ttgcactcat tgaagtaatg aatgaaacgc
    21961 ctaatatgat aaaagcaaca ggtggttttg cgaaaagtga agtatggcgt caaatgatgt
    22021 cagatatatt tgacacagag ttagtggttc ctgaaagtta tgaaagttca tgcttaggtg
    22081 cctgcgtgct tggacttaaa gctgtaggtg acattgaaga tttttcaatc gtttcatcga
    22141 tggtcggtgc tacaaataat catacgccgt ttgaagaaaa tgtcgctgtt taccaagagc
    22201 tcgtatccat ttttatcaat ttaagtcgtt ctttaacaga gaattatgaa caaattgcag
    22261 attttcaacg ccaacatatg gctgaaaata aaacacaata aatacgtcac tcaagcatct
    22321 tagataaagt tgttgtgcca tgactactat atattatctg gaatacgtgc gcgacgacgg
    22381 tgttacattc tattgaaaag agaaagaggg gaatgtcatg tttaacgaaa tatggccgtt
    22441 aatcagtgtt gttttaggta tcgttatttt attagtatta attattgggt ttaaattaaa
    22501 tacatttatt tcattaatta tcacatcgat gattacagcg ttaatgctag gtataccatt
    22561 gactaaaatt atggagacga ttgagaaagg gatgggcagt acgctcggcc acattgcact
    22621 aatattcggt ttaggtgcca tacttgggaa attacttgct gatggtggtg gtgcaactcg
    22681 aattgcagac acgttgattc aaaaatttgg tcaaaaacat gtacaatggg caatgcttgt
    22741 tgcagcattt atcgtcggta ttgcattgtt ctttgaagta ggtttagtct tactgattcc
    22801 attagtattt acagtagcaa aacgcgcaaa tgtttcagtg ttaaaactag gactacctat
    22861 ggtaacagct ttatctgtga cacatggctt tttaccacca catccaggac cggtagtcat
    22921 cgcaaaagaa ttaaaggcaa atgttggaga tgtattactg tacggtatga ttattgccat
    22981 tccagttaca ctcattgcag gacctatatt taacaaagtt gcacaaaaaa tgattccgtc
    23041 tgcgtataca cgagaaggcg atatttcagc gttaggtgca caaaaagaat ttacggatca
    23101 agagatgcca ggatttggta tgagtttatt aactgcaaca ttaccagtca tattaatgtt
    23161 agtgtctacg ataacgcaac ttgtaactgg gcacgacaaa cctacaaatc tatttgaatc
    23221 tatcatttat atgataggaa cagcagggac agccatgcta atagcagtat tatttgcaat
    23281 cgttacgatg ggattaatga gaaaacgtaa gatgaatcat attatggaat cagtgacgaa
    23341 tgcgatttat ccaatcggaa tgatgttatt gattattggc ggtggcggta catttaaaca
    23401 agtattaatt gacggtggtg tcggaaatac aattgctaaa atgtttgaag gtacagagat
    23461 gtctccaatt ttactagcat ggattgttgc agctgtgcta cgtatcgcat taggttcggc
    23521 tacagtagct gcgatttcaa ctacaggtat tgtcttacca ttattacaat catcagatgt
    23581 aaatgttgca ttagttgtac ttgcgatagg tgcaggtagt gtgattttgt ctcatgtaaa
    23641 tgacgcagga ttttggatgt ttaaagaata ttttggttta actgtaaaag aaacattctt
    23701 aacatggtca ttattagaaa cgattatttc cgtttcaggt atcatcttca ttctattcat
    23761 cagcttattt gtttaactaa atattaagta aacgtgtttt taggtaattg caatatattg
    23821 ccttgaaaca cgtttgtttt tatgcagaaa atcgtctgaa atagtcagaa tccgaacgat
    23881 ataaaaagta tattggggat tttagtgtaa tgaaaggcgt ttcaagacat gtttttgtgc
    23941 gtattgtaca ggcgataatt atgaaacact tagtatattg ttttaaatta gataatgatg
    24001 aatttaattt gaaaaataag tataaaaaat acaagccttg tgtgacaagg gcttatgatg
    24061 acttgaatac aatttatagg tatatttcaa ataataaaat tatcaattaa cataaaatta
    24121 atgacaatct taacttttca ttaactcgct tttttgtatt gcttttaaaa accgaacaat
    24181 atagacttgc atttattaag tttaaaaaaa ataatgaatt ttgcatttaa agggagatat
    24241 tatagtgaaa aacaatctta ggtacggcat tagaaaacat aaattgggag cagcatcagt
    24301 attcttagga acaatgatcg ttgttgggat gggacaagat aaagaagctg cagcatcaga
    24361 acaaaagaca actacagtag aagaaaatgg gaattcagct actgataata aaacaagtga
    24421 aacacaaaca actgctacta acgttaatca tatagaagaa actcaatcat ataacgcaac
    24481 agtaacagaa caaccgtcaa acgcaacaca agtaacaact gaagaagcac caaaagcagt
    24541 acaagcacca caaactgcac aaccagcaaa tgtagaaaca gttaaagaag aagagaaacc
    24601 tcaagttaag gaaacgacac aacctcaaga caatagcgga aatcaaagac aagtagattt
    24661 aacacctaaa aaggttacac aaaatcaagg gacagaaaca caagttgaag tggcacagcc
    24721 aagaacggca tcagaaagta agccacgtgt gacaagatca gcagatgtag cggaagctaa
    24781 ggaagctagt gacgtttcag aagttaaagg cacagatgtt acaagtaaag ttacagtaga
    24841 aagtggttct attgaggcac ctcaaggaaa taaagtagag ccacatgctg gtcaacgtgt
    24901 cgtattgaaa tacaaattga aattcgcaga tggattaaaa agaggagatt attttgattt
    24961 tacattatca aataatgtaa atacttatgg ggtttcaaca gctagaaagg taccagagat
    25021 taaaaatggc tcagttgtaa tggctacagg tgagatctta gggaatggta acataagata
    25081 tatatttact aacgaaattg aacacaaggt agaggtaaca gctaatttag aaatcaactt
    25141 atttattgac cctaaaactg tacaaagcaa tggagaacaa aagattactt ctaaattaaa
    25201 tggtgaagaa acagaaaaaa caataccagt tgtttataat ccaggtgtta gcaatagtta
    25261 tacaaatgta aatggatcaa ttgaaacatt taataaagaa tctaataaat ttacacatat
    25321 agcttatatt aagccaatga atggaaacca gtcaaacact gtatcagtaa cagggacgtt
    25381 gactgaaggt agtaatttag ctggtggaca acctactgtt aaagtatatg aatatctagg
    25441 gaaaaaagat gaattgccac aaagtgttta tgcaaataca tcagatacta acaaattcaa
    25501 agatgtaaca aaggaaatga atggaaaatt gagtgtgcaa gacaatggta gttactcatt
    25561 gaatttagat aagttggata aaacgtatgt cattcattat acaggtgaat atttgcaagg
    25621 gtcagatcag gttaatttta gaactgaatt atatgggtat ccagaacgag catataaatc
    25681 ttactatgtt tatgggggat atcgtttaac ttgggataat ggtttagttt tatatagcaa
    25741 taaagctgac ggcaatggta aaaatggaca aattattcaa gataatgatt ttgaatataa
    25801 agaagatact gcaaaaggaa ctatgagcgg gcagtacgat gccaagcaaa ttattgaaac
    25861 agaagaaaat caagacaata caccgcttga cattgattac cacacagcta tagatggtga
    25921 gggtggttat gttgatgggt atattgaaac aatagaagaa acggattcat cagctattga
    25981 tatcgattac catactgctg tggatagtga agcgggtcac gttggaggat acactgagtc
    26041 ctctgaggaa tcaaatccaa ttgactttga agaatcgaca catgaaaatt caaaacatca
    26101 cgctgatgtt gttgaatatg aagaggatac aaatccaggt ggtggccaag taacaactga
    26161 gtctaactta gttgaatttg acgaagagtc tacaaaaggt attgtaactg gcgcagtgag
    26221 cgaccataca acaattgaag atacgaaaga atatacgact gaaagtaatc tgattgaact
    26281 agtagatgaa ctacctgaag aacatggtca agcacaagga ccaatcgagg aaattactga
    26341 aaacaatcat catatttctc attctggttt aggaactgaa aatggtcacg gtaattatgg
    26401 cgtgattgaa gaaatcgaag aaaatagcca cgttgatatt aagagtgaat taggttacga
    26461 aggtggccaa aatagcggta accagtcatt cgaggaagac acagaagaag acaaacctaa
    26521 atatgaacaa ggtggcaata tcgtagatat cgatttcgac agtgtacctc aaattcatgg
    26581 tcaaaataaa ggtgaccagt cattcgaaga agatacagag aaagacaagc ctaaatatga
    26641 acatggcggt aatatcattg atatcgactt cgacagtgtg ccacaaattc atggattcaa
    26701 taagcataat gaaattattg aagaagatac aaacaaagat aaacctaatt atcaattcgg
    26761 tggacacaat agtgttgact ttgaagaaga tacacttcca aaagtaagcg gccaaaatga
    26821 aggtcaacaa acgattgaag aagatacaac gccgccaacg ccaccgacac cagaagtacc
    26881 gagtgagccg gaaacaccaa tgccaccgac accagaagta ccgagtgagc cggaaacacc
    26941 aacgccacca acaccagagg taccaagtga gccggaaaca ccaacaccac cgactccgga
    27001 agtaccaagt gagccggaaa caccaacacc accgacacca gaagtgccga gtgagccaga
    27061 aacaccaaca ccgccaacac cagaggtacc agctgaacct ggtaaaccag taccacccgc
    27121 aaaagaagaa cctaaaaagc cttctaaacc agtggaacaa ggtaaagtag taacacctgt
    27181 tattgaaatc aatgaaaagg ttaaagcagt ggcaccaact aaaaaagcac aatctaagaa
    27241 atctgaacta cctgaaacag gtggagaaga atcaacaaac aaaggtatgt tgttcggcgg
    27301 attattcagc attctaggtt tagcattatt acgcagaaat aaaaagaata acaaagcata
    27361 attaaacaaa aattgacggg tttatttcat aaattatatg aagtaaacct gtttttttag
    27421 attaaatcaa atttctaata gaaaattaga gtgttttctg attgctgaag cgctttatgt
    27481 ctgatgattg attacaaaat gagagataaa gttagaattt aaaactagtt tgttagtaca
    27541 aattagattg aatcaataag acaaatttga taaatgcaat tattaactag atgcaaattt
    27601 agttaaactt aaaatgtcgt cttgaaatac ggctgtttct gtataaataa tcattaaaat
    27661 aaaacaaaaa ccgaacgata taaaattata atgacgttat taatacaatg aaaggcattt
    27721 cagtgacttg ttaatgatgc attgtacagg cgattattat gtatg
//
LOCUS       DAHHSC010000015        26397 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.015, whole genome shotgun sequence.
ACCESSION   DAHHSC010000015 DAHHSC010000000
VERSION     DAHHSC010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 26397)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26397)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26397
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.015"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            100..2205
                     /gene="clpL"
                     /locus_tag="NBI57_000503"
     CDS             100..2205
                     /gene="clpL"
                     /locus_tag="NBI57_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpL"
                     /protein_id="HCD1821479.1"
                     /translation="MNNGFFNSDFDSIFRRMMQDMQGSNQVGNKKYYINGKEVSPEEL
                     AQLTQQGSNQSAEQSAQAFQQAAQRQQGQQGGNGNYLEQIGRNLTQEARDGLLDPVIG
                     RDKEIQETAEVLSRRTKNNPILVGEAGVGKTAIVEGLAQAIVEGNVPAAIKDKEIISV
                     DISSLEAGTQYRGAFEENIQKLIEGVKSSQNAVLFFDEIHQIIGSGATGSDSGSKGLS
                     DILKPALSRGEISIIGATTQDEYRNNILKDAALTRRFNEVLVNEPSAKDTVEILKGIR
                     EKFEEHHQVKLPDDVLKACVDLSIQYIPQRLLPDKAIDVLDITAAHLSAQSPAVDKVE
                     TEKRISELENDKRKAVSAEEYKKADDIQNEIKSLQDKLENSNGEHTAVATVHDISDTI
                     QRLTGIPVSQMDDNDIERLKNISNRLRSKIIGQDQAVEMVSRAIRRNRAGFDDGNRPI
                     GSFLFVGPTGVGKTELAKQLAIDLFGNKDALIRLDMSEYSDTTAVSKMIGTTAGYVGY
                     DDNSNTLTEKVRRNPYSVILFDEIEKANPQILTLLLQVMDDGNLTDGQGNVINFKNTI
                     IICTSNAGFGNGNDAEEKDIMHEMKKFFRPEFLNRFNGIVEFLHLDKDALQDIVNLLL
                     DDVQVTLDKKGITMDVSQDAKDWLIEEGYDEELGARPLRRIVEQQVRDKITDYYLDHT
                     DVKHVDIDVEDKELVVKGK"
     gene            complement(2274..2444)
                     /locus_tag="NBI57_000504"
     CDS             complement(2274..2444)
                     /locus_tag="NBI57_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HCD1821480.1"
                     /translation="MSVIINILIFLAIFGYALYTLVKFFKRSKQGKCGTCDINRDCCG
                     IEQHTANHFPGK"
     gene            complement(2457..4451)
                     /gene="feoB"
                     /locus_tag="NBI57_000505"
     CDS             complement(2457..4451)
                     /gene="feoB"
                     /locus_tag="NBI57_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HCD1821481.1"
                     /translation="MENYCILGNPNVGKTSLFNALTGSYEYIGNWSGVTVEKKVGKLK
                     ENVGQLIDLPGTYDLSPISKDETVVTDYLLNDSFSGIINIVDASQLKRNVQLTVQLLE
                     LNQPIYIGLNMIDVATKRGIKIDYHKLMKKLKTPIFPVVARTGKGTKYLLGEIKHLGE
                     GYQPHFKINYGEKIEETIKNMCQIIMTETSHDKYQARFIAIQFLLNNMQIANELNSEV
                     VNKLSSLRDQVAEQVGAVSVRREMERIRNHYIETLLQDVVTYPDEDKQYFSSRIDKIL
                     THKYIGMPIFLAIMWLIFQTTFTWIGTPLSDQLDAFIGGTFTDSVKTIMNYLGVIPFL
                     QDLITDGIIAGVGSVLVFVPQIVVLFFFISLLEDSGYMARIAVLMDRIMESFGLSGKS
                     FIPMIIGFGCNVPSIMAARSIENEKERLTTILIAPFMSCSARLPVYALFVGIFFKENQ
                     SLVVLSLYVLGIIMAFLVSTVLTKTILKNDNAIFIVELPTYRVPSIKTLWRSTWEKAK
                     GFVRKAGTFIFGGSVVIWLLSYVGPHGINVNINQSFLHMVGSFFGMLVQPLGFGTWQA
                     GATLVPGFLAKEVIVSSMAIIYSSGDAGLVNVIQNQFTPLSAYAFMIFILLYIPCVST
                     VAAIRKETYSWKWTALAVAYPLVTAYVLTFIFYQVGHLFV"
     gene            complement(4463..4690)
                     /locus_tag="NBI57_000506"
     CDS             complement(4463..4690)
                     /locus_tag="NBI57_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HCD1821482.1"
                     /translation="MLNIKNGEMNKAYKIKRMDIANENMLYRLSAFGLTDDAIITIKQ
                     KCLFKGPCIIEVNGQQLSIRHCDACSIALEE"
     gene            complement(4906..7395)
                     /gene="farE"
                     /locus_tag="NBI57_000507"
     CDS             complement(4906..7395)
                     /gene="farE"
                     /locus_tag="NBI57_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001051413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid efflux MMPL transporter FarE"
                     /protein_id="HCD1821483.1"
                     /translation="MAKFLYKMGTFIAKHKWSAVIAWIVIVAAILIPLATNAPKFDND
                     IKMTGLESLDTNKKIEKHFNQDSEKAQIRVVFKTTKDDGIVQPNITEDIKKTLEDIKK
                     DDKHIDKISDPYENKQISKDKTTAFADITYDVSQTSLKDGSRDNVKSHLKDLRDNHNV
                     QTELTGTGMTSTEVGGNSELVGIIVAFVVLLITFGSVIAAGLPIISALIGLASGVGII
                     SLLTYAFDIPNVTLTLAVMIGLAVGIDYALFILFRYRQVMKTETDYIKGIGLAIGTAG
                     SAVVFAGVTVVIAVCGLSLVGIDFLAVMGFASAISVIFAVFSALTLLPALISIFHKRI
                     KVNKLQSNFKKDIDTPWSKFITGNALAAVLLGLIILVAAAIPVSHMRLGIPDDGVKPA
                     DSTQKKAYDIISDKFGEGFNGQIPMLINVKDKKDDPQGLQQDLQSVYKDIKDKKNVDI
                     VTPPQMSKDNDYALMVVIPKQGPNAESTNDLVHDLRDYHKDAQDKYGFKTEISGQSVI
                     NIDMSKKLNEAIPLFATVIVVLAFFLLMIVFRSILIPLKAVLGFVLSLMATLGFTTLV
                     MQDGFMKGLFGIETTGPMLAFLPVITIGILFGLAMDYEVFLMSRIHEEYSKTGDNDYS
                     IKVGLKESGPVIVAAALIMFSVFFAFVFQEDVMIKSMGMALAFGVLFDAFVVRMMLIP
                     ALTKLFGKGSWYLPAWLNRIIPRVDIEGHALEKYKTVESQESEAKDSKETYDTTFKVY
                     PQGATNVSKHQDVHGQDDAHSIVLDDKTMALYQEVKQQSASSLFLYDALIDYQNKHQL
                     NSKQQVTNIEQLNKNIEKLNQLLEKNLRNKS"
     gene            7540..8088
                     /gene="farR"
                     /locus_tag="NBI57_000508"
     CDS             7540..8088
                     /gene="farR"
                     /locus_tag="NBI57_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid efflux pump transcriptional regulator
                     FarR"
                     /protein_id="HCD1821484.1"
                     /translation="MKETDLRVIKTKKALSSSLLQLLEQQLFQTITVNQICDNALVHR
                     TTFYKHFYDKYDLLEYLFNQLTKDYFARDISDRLNHPFQTMSDTINNKEDLREIAEFQ
                     EEDAEFNKVLKNVCIKIMHNDIKNNRDRIDIDSDIPDNLIFYIYDSLIEGFIHWIKDE
                     KIDWPGEDIDNIFHRLINIKIK"
     gene            complement(8165..8332)
                     /locus_tag="NBI57_000509"
     CDS             complement(8165..8332)
                     /locus_tag="NBI57_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1821485.1"
                     /translation="MRYVISIIMGIVLAIWSYKQLSQSHLDSGFIFFFIVYVLCISYF
                     NNDKHDKNKKR"
     gene            complement(8444..9988)
                     /gene="pruA"
                     /locus_tag="NBI57_000510"
     CDS             complement(8444..9988)
                     /gene="pruA"
                     /locus_tag="NBI57_000510"
                     /EC_number="1.2.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-glutamate gamma-semialdehyde dehydrogenase"
                     /protein_id="HCD1821486.1"
                     /translation="MVVEFKNEPGYDFSVQENVDMFKKALKDVEKELGQDIPLVINGE
                     KIFKDDKIKSINPADTSQVIANASKATKQDVEDAFKAANEAYKSWKTWSANDRAELML
                     RVSAIIRRRKAEIAAIMVYEAGKPWDEAVGDAAEGIDFIEYYARSMMDLAQGKPVLDR
                     EGEHNKYFYKSIGTGVTIPPWNFPFAIMAGTTLAPVVAGNTVLLKPAEDTPYIAYKLM
                     EILEEAGLPKGVVNFVPGDPKEIGDYLVDHKDTHFVTFTGSRATGTRIYERSAVVQEG
                     QNFLKRVIAEMGGKDAIVVDENIDTDMAAEAIVTSAFGFSGQKCSACSRAIVHKDVYD
                     EVLEKSIKLTKELTLGNTVDNTYMGPVINKKQFDKIKNYIEIGKEEGKLEQGGGTDDS
                     KGYFVEPTIISGLKSKDRIMQEEIFGPVVGFVKVNDFDEAIEVANDTDYGLTGAVITN
                     NREHWIKAVNEFDVGNLYLNRGCTSAVVGYHPFGGFKMSGTDAKTGSPDYLLHFLEQK
                     VVSEMF"
     gene            complement(10178..10777)
                     /locus_tag="NBI57_000511"
     CDS             complement(10178..10777)
                     /locus_tag="NBI57_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HCD1821487.1"
                     /translation="MTEKEKMLAEKWYDANFDQDLINERARAKDICFELNHTKPSDTN
                     KRKELIDQLFQTTTDNVSISIPFDTDYGWNVKLGKNVYVNTNCYFMDGGQITIGDNVF
                     IGPNCGFYTATHPLNFYHRNEGYEKAGPIHIGSNTWFGGHVAVLPGVTIGEGSVIGAG
                     SVVTKDIPPHSLAVGNPCKVVRKIDNDLPSETLNDETIK"
     gene            11016..11207
                     /gene="cwrA"
                     /locus_tag="NBI57_000512"
     CDS             11016..11207
                     /gene="cwrA"
                     /locus_tag="NBI57_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall inhibition responsive protein CwrA"
                     /protein_id="HCD1821488.1"
                     /translation="MRILITGTVAILIILGLVKTIQDYEMTNDTSRQLSDNKDDDKVI
                     HLNNFKNLHAKEFNPSDFF"
     gene            11447..13855
                     /locus_tag="NBI57_000513"
     CDS             11447..13855
                     /locus_tag="NBI57_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HCD1821489.1"
                     /translation="MANTKKTTLDITGMTCAACSNRIEKKLNKLDDVNAQVNLTTEKA
                     TVEYNPDQHDVQEFINTIQHLGYGVTVETVELDITGMTCAACSSRIEKVLNKMNGVQN
                     ATVNLTTEQAKVDYYPEETDADKLVTRIQKLGYDASIKDNNKDQTSRKAEALQHKLIK
                     LIISAVLSLPLLMLMFVHLFNMHIPALFTNPWFQFILATPVQFIIGWQFYVGAYKNLR
                     NGGANMDVLVAVGTSAAYFYSIYEMVRWLNGSTTQPHLYFETSAVLLTLILFGKYLEA
                     RAKSQTTNALGELLSLQAKEARILKDGNEVMIPLNEVHVGDTLIVKPGEKIPVDGKII
                     KGMTAIDESMLTGESIPVEKNVDDTVIGSTMNKNGTITMTATKVGGDTALANIIKVVE
                     EAQSSKAPIQRLADIISGYFVPIVVGIALLIFIVWITLVTPGTFEPALVASISVLVIA
                     CPCALGLATPTSIMVGTGRAAENGILFKGGEFVERTHQIDTIVLDKTGTITNGRPVVT
                     DYHGDNQTLQLLATAEKDSEHPLAEAIVNYAKEKQLTLTETTTFKAVPGHGIEATIDH
                     HHILVGNRKLMADNDISLPKHISDDLTHYERDGKTAMLIAVNYSLTGIIAVADTVKDH
                     AKDAIKQLHDMGIEVAMLTGDNKNTAQAIAKQVGIDTVIADILPEEKAAQIAKLQQQG
                     KKVAMVGDGVNDAPALVKADIGIAIGTGTEVAIEAADITILGGDLMLIPKAIYASKAT
                     IRNIRQNLFWAFGYNIAGIPIAALGLLAPWVAGAAMALSSVSVVTNALRLKKMRLEPR
                     RKDA"
     gene            14266..14472
                     /gene="copZ"
                     /locus_tag="NBI57_000514"
     CDS             14266..14472
                     /gene="copZ"
                     /locus_tag="NBI57_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone CopZ"
                     /protein_id="HCD1821490.1"
                     /translation="MSQEILNVEGMSCGHCKSAVESALNNIDGVTSADVNLENGQVSV
                     QYDDSKVAVSQMKDAIEDQGYDVV"
     gene            complement(14563..15561)
                     /locus_tag="NBI57_000515"
     CDS             complement(14563..15561)
                     /locus_tag="NBI57_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HCD1821491.1"
                     /translation="MTKIKIMSVRDEDMPYIKAWAEKHHVEVDITKEALTDDNVEGVA
                     GYDGLSLSQQIPLSEHVYKRLNELGIKQIAQRSAGFDTYDLELANKYNLIVSNVPSYS
                     PNSIAEFAVNQAINVVRHFNQIQTKVREHDFRWEPTILSKSIKDLKVAVIGTGRIGRV
                     VADIFANGYQSDVVAYDPFPNAKIATYVDYKDTIEEAVEGADIVTLHVPATKYNHYLF
                     NAELFKHFKKGAVFVNCARGSLVDTKALLDALDNGVIKGAALDTYEFERKLFPSDQRG
                     KTPNDPLLESLIDREDVILTPHIAFYTEAAVKNLIVDALDATLDVLQTGDTRLRVN"
     gene            complement(15584..16738)
                     /locus_tag="NBI57_000516"
     CDS             complement(15584..16738)
                     /locus_tag="NBI57_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HCD1821492.1"
                     /translation="MISNKLANIPDSYFGKTMGRKIEHGPLPLINMAVGIPDGPTPQG
                     IIDHFQKALTIPENQKYGAFHGKEAFKQAIVDFYQRQYNVTLDKEDEVCILYGTKNGL
                     VAVPTCVINPGDYVLLPDPGYTDYLAGVLLADGKPVPLNLEPPHYLPDWSTVDSQIID
                     KTKLIYLTYPNNPTGSTATKEVFDEAIAKFKGTDTKIVHDFAYGAFGFDAKNPSILAS
                     ENGKDVAIEIYSLSKGYNMSGFRVGFAVGNKDMIQALKKYQTHTNAGMFGALQDAAIY
                     ALNHYDDFLEEQSNVFKTRRDRFEAMLAKADLPFVHAKGGIYVWLETPPGYDSEQFEQ
                     FLVQEKSILVAPGKPFGENGNRYVRISLALDDQKLDEAAIRLTELAYLYE"
     gene            complement(17166..18674)
                     /locus_tag="NBI57_000517"
     CDS             complement(17166..18674)
                     /locus_tag="NBI57_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HCD1821493.1"
                     /translation="MKIAVIGAGVTGLAAAARIASQGHEVTIFEKNNNVGGRMNQLKK
                     DGFTFDMGPTIVMMPDVYKDVFTACGKNYEDYIELRQLRYIYDVYFDHDDRITVPTDL
                     AELQQMLESIEPGSTHGFMSFLTDVYKKYEIARRYFLERTYRKPSDFYNMTSLVQGAK
                     LKTLNHADQLIEHYIDNEKIQKLLAFQTLYIGIDPKRGPSLYSIIPMIEMMFGVHFIK
                     GGMYGMAQGLAQLNKDLGVNIELNAEIEQIIIDPKFKRADAIKVNGDIRKFDKILCTA
                     DFPSVAESLMPDFAPIKKYPPHKIADLDYSCSAFLMYIGIDIDVTDQVRLHNVIFSDD
                     FRGNIEEIFEGRLSYDPSIYVYVPAVADKSLAPEGKTGIYVLMPTPELKTGSGIDWSD
                     EALTQQIKEIIYRKLATIEVFEDIKSHIVSETIFTPNDFEQTYHAKFGSAFGLMPTLA
                     QSNYYRPQNVSRDYKDLYFAGASTHPGAGVPIVLTSAKITVDEMIKDIERGV"
     gene            complement(18686..19549)
                     /locus_tag="NBI57_000518"
     CDS             complement(18686..19549)
                     /locus_tag="NBI57_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene/squalene synthase family protein"
                     /protein_id="HCD1821494.1"
                     /translation="MTMMDMNFKYCHKIMKKHSKSFSYAFDLLPEDQRKAVWAIYAVC
                     RKIDDSIDVYGDIQFLNQIKEDIQSIEKYPYEHHHFQSDRRIMMALQHVAQHKNIAFQ
                     SFYNLIDTVYKDQHFTMFETDAELFGYCYGVAGTVGEVLTPILSDHETHQTYDVARRL
                     GESLQLINILRDVGEDFDNERVYFSKQRLKQYEVDIAEVYQNGVNNHYIDLWEYYAAI
                     AEKDFQDVMDQIKVFSIEAQPIIELAARIYIEILDEVRQANYTLHERVFVDKRKKAKL
                     FHEINSKYHRI"
     gene            complement(19586..20713)
                     /gene="crtQ"
                     /locus_tag="NBI57_000519"
     CDS             complement(19586..20713)
                     /gene="crtQ"
                     /locus_tag="NBI57_000519"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4,4'-diaponeurosporenoate glycosyltransferase"
                     /protein_id="HCD1821495.1"
                     /translation="MKWLSRILTVIVTMSMACGALIFNRRHQLKTKTLNFNHKALTII
                     IPARNEEKRIGHLLHSIIQQQVPVDVIVMNDGSTDETARVARSYGATVVDVVDDTDGK
                     WYGKSHACYQGVTHACTNRIAFVDADVTFLRKDAVETLINQYQLQGEKGLLSVQPYHI
                     TKRFYEGFSAIFNLMTVVGMNVFSTLDDGRTNQHAFGPVTLTNKEDYYATGGHKSANR
                     HIIEGFALGSAYTSQSLPVTVYEGFPFVAFRMYQEGFQSLQEGWTKHLSTGAGGTKPK
                     IMTAIVLWLFGSIASILGLCLSLKYRQMSVRKMVALYLSYTTQFIYLHRRVGQFSNLL
                     MVCHPLLFMFFTKIFIQSWKQTHRYGVVEWKGRQYSISKEQ"
     gene            complement(20719..22212)
                     /locus_tag="NBI57_000520"
     CDS             complement(20719..22212)
                     /locus_tag="NBI57_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HCD1821496.1"
                     /translation="MTKHIIVIGGGLGGISAAIRMAQSGYSVSLYEQNTHIGGKVNRH
                     ESDGFGFDLGPSILTMPYIFEKLFEYSKKQMSDYVTIKRLPHQWRSFFPDGTTIDLYE
                     GIKETGQHNAILSKQDIEELQNYLNYTRRIDRITEKGYFNYGLDTLSQIIKFHGPLNA
                     LINYDYVHTMQQAIDKRISNPYLRQMLGYFIKYVGSSSYDAPAVLSMLFHMQQEQGLW
                     YVEGGIHHLANALEKLAREEGVTIHTGARVDNIKTYQRRVTGVRLDTGEFVKADYIIS
                     NMEVIPTYKYLIHLDTQRLNKLEREFEPASSGYVMHLGVACQYPQLAHHNFFFTENAY
                     LNYQQVFHEKVLPDDPTIYLVNTNKTDHTQAPVGYENIKVLPHIPYIQDQPFTTEDYA
                     KFRDKILDKLEKMGLTDLRKHIIYEDVWTPEDIEKNYRSNRGAIYGVVADKKKNKGFK
                     FPKESQYFENLYFVGGSVNPGGGMPMVTLSGQQVADKINAREAKNRK"
     gene            complement(22205..22693)
                     /gene="crtO"
                     /locus_tag="NBI57_000521"
     CDS             complement(22205..22693)
                     /gene="crtO"
                     /locus_tag="NBI57_000521"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl-4,4'-diaponeurosporenoate
                     acyltransferase"
                     /protein_id="HCD1821497.1"
                     /translation="MKKYIKTAFFCSMYWLIVQLNIANLGTRIPDKYFRQKYIIFKSF
                     NFEKHGKFWNKWFYVRKWKHKILDGHQLNQNIYDQRHLMTINTDEIEKMIIETKRAEL
                     IHWISILPVIIFNKGSRLVKYINIFYAMIANVPIIIVQRYNRPRLTQLLRILKRRGER
                     HD"
     gene            complement(22868..23635)
                     /locus_tag="NBI57_000522"
     CDS             complement(22868..23635)
                     /locus_tag="NBI57_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HCD1821498.1"
                     /translation="MKKIVTATIATAGLATIAFAGHDAQAAEQNNNGYNSNDAQSYSY
                     TYTIDAQGNYHYTWTGNWNPSQLTQNNTYYYNNYNTYSYNNASYNNYYNHSYQYNNYT
                     NNSQTATNNYYTGGSGASYSTTSNNVHVTTTAAPSSNGRSISNGYASGSNLYTSGQCT
                     YYVFDRVGGKIGSTWGNASNWANAAASSGYTVNNTPKVGAIMQTTQGYYGHVAYVEGV
                     NSNGSVRVSEMNYGHGAGVVTSRTISANQAGSYNFIH"
     gene            complement(23996..25807)
                     /locus_tag="NBI57_000523"
     CDS             complement(23996..25807)
                     /locus_tag="NBI57_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HCD1821499.1"
                     /translation="MDTKDFKRLEKMYSPRYLPGLDGLRAFAVIGIIIYHLNAQWLSG
                     GFLGVDTFFVISGYLITSLLISEYYRTQKIDLLEFWKRRLKRLIPAVLFLICVVLTFT
                     LIFKPELIIQMKRDAIAAIFYVSNWWYISQNVDYFNQFAIEPLKHLWSLAIEEQFYLL
                     FPLVITFLLHRFKPRNIIQTLFIVSLISLGLMIVIHFITGDNSRVYFGTDTRLQTLLL
                     GCILAFIWPPFALKKDISKKIVVSLDIIGISGFAVLMTLFFIVGDQDQWIYNGGFYII
                     SFATLFIIAIAVHPSSLFAKFLSMKPLLIIGKRSYSLYLWHYPIIVFVNSYYVQGQIP
                     VYVYIIEILLTALMAEISYRFIETPIRKKGFKAFAFLPKKKGQFARTVLVILLLVPSI
                     VVLSGQFDALGKQHEAEKKEKKTEFKTTKKKVVKKDKQEDKQTANSKEDIKKSSPLLI
                     GDSVMVDIGNVFTKKIPNAQIDGKVGRQLVDATPIVKSQYKDYAKKGQKVVVELGTNG
                     AFTKDQLNELLDSFGKADIYLVSIRVPRDYEGRINKLIYEAAEKRSNVHLVDWYKASA
                     GHPEYFAYDGIHLEYAGSKALTDLIVKTMETHATNKK"
     gene            complement(26320..>26397)
                     /locus_tag="NBI57_000524"
     CDS             complement(26320..>26397)
                     /locus_tag="NBI57_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCD1821500.1"
                     /translation="KRGDLPSSFPKLDVDNRFYYRNLKD"
BASE COUNT         8519 a         5001 c         4046 g         8831 t
ORIGIN      
        1 ttagtcgaaa agtacaattg caattgtata tagtttgcat aacgcttcaa aagtaatttc
       61 ttttttgttt agttcaaaaa aatttagagg tgatgttata tgaataacgg ttttttcaat
      121 agcgactttg attcaatttt tcgaagaatg atgcaagata tgcaaggttc aaatcaagtc
      181 ggtaacaaaa agtactatat taatggtaaa gaagtttcac ctgaagaact agcgcaactc
      241 acacaacaag gtagcaatca atctgctgaa caaagtgcgc aagcttttca acaagcagca
      301 caaagacaac aagggcaaca aggtggcaac ggcaattatt tagaacaaat tggtcgtaac
      361 cttacgcaag aagcacgtga cggtttatta gatccagtca ttggtcgtga taaagaaatt
      421 caagaaactg ctgaagtctt aagtagacga actaaaaaca atcctatatt agttggagaa
      481 gctggtgttg gtaaaactgc gattgttgaa ggtttagcac aggcaatcgt tgaaggaaat
      541 gtaccagcag caatcaaaga caaagaaatt atttctgtag atatttcatc attagaagct
      601 ggaacgcaat atcgtggtgc ttttgaagaa aatattcaaa aattaatcga aggtgttaaa
      661 tcttcacaaa atgccgtact attctttgat gaaatccatc aaattatcgg ttcaggtgcc
      721 acaggaagtg attcaggtag caaagggtta tctgatattt tgaaacctgc attaagtcgt
      781 ggtgagattt ctattattgg tgcaacaaca caagatgaat atcgaaacaa tattcttaaa
      841 gatgctgcat taacgcgcag atttaatgaa gtgcttgtta atgaaccaag cgctaaagat
      901 actgttgaaa ttttaaaagg tattcgcgaa aaattcgaag aacaccatca agtaaaatta
      961 ccagatgacg tattaaaagc atgtgttgac ttatcaattc aatatattcc acaacgatta
     1021 ttaccagata aagcaatcga tgtgttagat attacagcag cacatttatc tgcgcaaagt
     1081 ccagctgtcg ataaagttga aactgaaaaa cgaatttctg aattagaaaa tgataaacgt
     1141 aaagcagtaa gtgctgaaga atataaaaaa gctgacgaca ttcaaaatga aatcaaatca
     1201 ttacaagata aattagaaaa cagtaatggt gaacatactg ctgttgctac agttcatgat
     1261 atttcagata ctattcaacg attaactggc attccagttt ctcaaatgga tgataacgat
     1321 attgaacgtt taaaaaatat ttctaatcgt ttaagaagta aaatcatagg tcaagatcaa
     1381 gctgtagaaa tggtttcacg tgcaattcgc cgtaatcgcg ctgggtttga tgacggcaac
     1441 cgtccaattg gtagtttctt atttgtcggc cctactggtg ttggtaaaac agagcttgct
     1501 aaacaattag caattgatct atttggtaat aaagatgcac ttattcgact tgatatgagt
     1561 gaatatagtg acacaacagc tgtttcaaaa atgattggta caactgctgg ttatgtcggt
     1621 tatgatgaca attcaaatac gttaactgaa aaagtacgcc gtaatccata ctcagtcatt
     1681 ctatttgatg aaatcgaaaa agcaaatcca caaattttaa cattgttatt acaagtaatg
     1741 gatgatggta atttgactga tggtcaaggt aatgtcatca actttaaaaa tacaattatt
     1801 atttgtacat caaatgctgg ctttggcaat ggcaatgacg ctgaagaaaa agatattatg
     1861 cacgaaatga aaaaattctt ccgccctgaa ttccttaacc gcttcaacgg catcgttgaa
     1921 ttcttacatt tagataaaga tgcattgcaa gatatcgtca acttattatt agacgatgta
     1981 caagttacat tagataaaaa aggtattacg atggacgttt ctcaagatgc gaaagattgg
     2041 ttaattgaag aaggctatga tgaagaatta ggtgcacgtc cattaagacg tattgttgaa
     2101 cagcaagtac gtgacaaaat tacagattat tatctagatc atacagacgt taaacatgtg
     2161 gatatagatg ttgaggataa agaattagtc gtaaaaggta aataacgaca ctttaacata
     2221 tcgcgcatca aaaatgagca tcaggtcgcc cttgcctgtg ctcatttttt taattatttc
     2281 cctggaaaat gattcgctgt gtgctgttct attccacaac aatcacgatt aatgtcacat
     2341 gtaccacatt ttccttgttt cgaacgcttg aaaaatttta ctagtgtata taaggcatat
     2401 ccgaaaattg ctaaaaaaat taaaatgtta ataatgactg acactttaac cactccttaa
     2461 acaaataaat gtccgacttg ataaaaaatg aatgttaaga catatgcagt gactagagga
     2521 taggcaactg caagtgccgt ccatttccat gaataagtct ctttacggat tgctgccact
     2581 gtagaaacac aaggaatata caatagtata aatatcataa atgcatacgc agatagcggt
     2641 gtgaattgat tttgaatcac attaacaagg cctgcatcac ctgatgaata gataatcgcc
     2701 atcgaactta cgataacttc ttttgctaaa aatcctggca ctaacgtagc acctgcttgc
     2761 catgttccaa atccgagcgg ttgcactaac ataccaaaga aactaccaac catatgtaaa
     2821 aaactttgat tgatattcac attgatacca tgtggtccta cataacttaa tagccaaatg
     2881 actactgagc cgccaaaaat aaatgtacct gctttacgaa caaagccctt agccttttcc
     2941 caagtactac gccacaacgt tttaatggaa ggcacacggt atgttggcaa ttccacaata
     3001 aagattgcat tatcattttt taaaatcgtc ttagtaagta ctgtactgac taaaaatgcc
     3061 ataataatac ctaaaacata caggcttaat actactaaag attgattctc tttgaaaaag
     3121 atacctacga acaacgcata cactggcagt ctagcagagc atgacatgaa tggtgcaatt
     3181 aatatcgttg ttaaacgctc tttttcattt tcaatactgc gcgcagccat aatactcggt
     3241 acattacaac caaatccgat aatcattggt ataaaagact tcccgcttaa accgaacgat
     3301 tccataatac gatccattaa aaccgcaata cgtgccatat aacctgaatc ttctaataaa
     3361 gatataaaga aaaagagcac aacaatttgt ggtacaaaga ctaatactga tcctacacca
     3421 gcaataatgc catctgtaat taaatcttgt aaaaatggta taacccctag ataattcata
     3481 atcgtcttca cactatctgt aaatgtacca cctataaatg catcgagttg atccgacaaa
     3541 ggtgtgccaa tccatgtaaa tgtagtttga aagatcaacc acataattgc tagaaagata
     3601 ggcatcccta tatatttatg tgttaatatc ttgtctattc tagagctgaa atattgctta
     3661 tcttcatctg gatacgttac cacgtcttgc aataacgtct caatataatg attgcgtata
     3721 cgctccatct ctcgacgaac agatacagcc cctacttgtt cagcaacttg atcacgtaaa
     3781 ctcgacaatt tatttacaac ctctgaatta agttcgtttg caatttgcat gttatttaat
     3841 aagaattgaa tcgcaataaa cctagcttga tacttatcat gagatgtctc tgtcattatt
     3901 atttgacaca tatttttaat tgtctcttca atcttctcac cataattgat tttaaaatgc
     3961 ggttgatacc cttcccctag atgctttatt tcgccaagta aatattttgt tcctttgcct
     4021 gtacgtgcca caactggaaa aataggtgtt tttaactttt tcatcaattt atgataatcg
     4081 atttttatcc cgcgctttgt agctacatca atcatattta atccgatgta tattggttga
     4141 ttaagttcta acaattgtac tgttaattgc acatttcttt ttagttgact cgcatcaaca
     4201 atgttaatga ttcctgaaaa tgaatcgttt aataaatagt ctgtcactac agtttcatct
     4261 ttagaaatcg gcgataaatc atatgtacct ggtaaatcaa ttaattgtcc tacattttct
     4321 ttaagtttcc ctactttttt ctctaccgtt acgccactcc agttgcctat atattcatac
     4381 gaaccagtta aagcgttaaa caaagatgtt ttaccaacat taggatttcc taaaatacaa
     4441 taattttcca ttcgtccggc tcctattctt ctaatgcaat agaacaagca tcgcaatgtc
     4501 taatacttaa ctgttgtccg tttacttcaa taatacatgg ccctttaaat aaacattttt
     4561 gtttaatcgt tatgatagcg tcatctgtta acccaaaggc acttagacga tacaacatat
     4621 tctcattagc aatatccatt cgctttattt tataagcctt attcatttca ccatttttaa
     4681 tgtttaacat actatttcgc tctcctatta gaaataataa tcattatcac ttaaaaatca
     4741 taacccttaa aattgtagct cgcaatactt tatttaaata attttcattt ttcatgtaaa
     4801 atttgcgaca ttgcaaaaat gttttgtata atcacttcca tgcaaaaaac ctccgcttca
     4861 aatgtcattg atagacattt aaagcggagg catgtacatg ttattttatg atttatttcg
     4921 tagatttttt tctaacaatt gattgagctt ttcaatattt ttattcaact gttctatatt
     4981 cgttacttgt tgttttgaat ttagttgatg cttgttttga taatctatta aagcatcata
     5041 taagaacaat gaacttgcag attgctgttt cacttcttgg tacaatgcca tcgttttatc
     5101 atctagtact attgaatgtg catcatcttg cccatgtaca tcttgatgtt tcgatacatt
     5161 tgttgcacct tgtggatata ctttaaaggt tgtgtcgtat gtttccttac tatctttggc
     5221 ttcagattcc tgtgattcaa ccgttttata tttttcaagt gcatgtcctt caatatcaac
     5281 tcgtggaata atgcgattca accatgctgg taaataccac gaaccttttc caaacaattt
     5341 cgttaatgca ggaattaaca tcattctgac tacgaaggca tcaaagagta caccaaacgc
     5401 taatgccata cccattgatt taatcatgac atcttcttgg aatacaaacg caaagaagac
     5461 actaaacata attaatgcag cggctacaat aacaggaccg ctttctttca atcctacttt
     5521 gatagaataa tcattatccc ctgttttact atattcttca tgaattcgcg acataaggaa
     5581 gacttcataa tccatcgcta atccaaataa gatacctata gtaataaccg gtaaaaatgc
     5641 tagcattggt cctgtcgttt caataccaaa cagacctttc ataaaaccat cttgcattac
     5701 taatgttgta aatcctagcg ttgccattaa tgacaagaca aatcctaaaa ctgcttttaa
     5761 cggtattaga attgaacgga agacaatcat taataagaaa aatgccaata caacaattac
     5821 tgttgcaaat aaaggtatcg cctcatttaa ctttttagac atatcaatat taatgacact
     5881 ttgtcccgaa atctccgttt tgaacccata tttatcttgt gcatctttat gataatctcg
     5941 taaatcatgc actaaatcat ttgtgctctc tgcattaggc ccttgcttag gtatcacgac
     6001 catcaaagca taatcattat ctttactcat ttgtggcggc gtaacgatat ctacattttt
     6061 cttatcttta atatctttat atacagactg taaatcttgt tgtaatcctt gtggatcatc
     6121 ttttttatct ttaacattta tcaacatcgg aatttggcca ttaaatcctt caccaaattt
     6181 atccgagatt atatcgtaag cttttttctg tgtagaatct gctggtttaa caccgtcatc
     6241 tggaatacca agtcgcatat gactaactgg tattgcagct gctactaata tgattaaacc
     6301 tagtaatact gccgcaagtg catttcctgt aataaattta gaccacggcg tatcaatatc
     6361 ttttttaaaa ttagactgca atttgttcac tttaatgcgt ttatggaaaa tactaattaa
     6421 cgcaggtaat aaagtcagtg cactgaatac agcaaatata acactaattg ccgaagcaaa
     6481 ccccatcact gctaagaaat ctatgccaac tagcgataaa ccacatacgg caatcacaac
     6541 tgtcacacca gcaaaaacaa ctgcactacc tgctgttcct attgcaagac caatgccttt
     6601 aatgtaatct gtttcagttt tcataacttg tcgatacctg aataaaataa acaaagcata
     6661 atcgatacca actgctagtc caatcattac ggctaatgtc aatgtgacat ttggtatatc
     6721 aaatgcataa gttaacaaac tgataatacc tacaccagag gctagaccaa tcaatgcact
     6781 tataattggt aatcctgcag caatgactga accgaatgtg attaacagta caacaaatgc
     6841 aacaataata ccaactagtt cagaattacc gcctacttct gtagatgtca ttcctgtacc
     6901 agttaactct gtttgtacat tatgattgtc acgtaagtct ttcaaatgac ttttaacatt
     6961 atctctagag ccatctttta aagatgtttg actaacgtca tatgtgatat ctgcaaatgc
     7021 agttgtttta tctttactaa tttgcttatt ttcatatgga tctgatattt tatcaatgtg
     7081 cttgtcatct ttttttatat cctctaatgt tttcttaatg tcttctgtaa tgttcggttg
     7141 cacaatacca tcatctttag tcgtcttaaa gacaacacgt atttgtgcct tttcactatc
     7201 ttgattaaaa tgtttttcaa tctttttatt cgtatctaac gactctaatc ctgtcatttt
     7261 aatatcattg tcaaatttcg gtgcatttgt agcaagtggt atcaatattg cagctacaat
     7321 cactatccat gcaatgaccg cggaccattt atgttttgcg atgaatgtcc ccatcttata
     7381 taaaaatttt gccaaagtat attgcctcct tttaaaatca acgttatagt ttaaatatac
     7441 agtgtagatt attgttcgat tatagtatct atccccgacc tcttaaagaa tcaattggaa
     7501 aattttgtat attaaactac acacaaagga gaaatgtaga tgaaagagac tgatttacga
     7561 gttataaaga caaaaaaagc attgtcgagt agcttgctac aattgttaga acagcaatta
     7621 ttccaaacga ttactgtcaa tcaaatttgc gacaatgcac tcgtacaccg cacaacattt
     7681 tataaacatt tttatgataa atatgatctt ctagagtatt tgttcaatca attgactaaa
     7741 gactactttg ctagagatat cagtgaccgt cttaatcatc cattccaaac gatgagtgat
     7801 acgattaata ataaagagga tttgagagaa atcgcagaat tccaagaaga agacgctgaa
     7861 tttaataaag tattaaaaaa tgtctgcatt aaaattatgc ataacgatat caaaaataat
     7921 agagaccgta tcgatattga cagcgacatc ccagataatc tcatatttta tatttatgac
     7981 tcgttgattg aaggttttat acattggata aaagatgaaa aaattgactg gcctggcgaa
     8041 gatattgata acattttcca tagattaatc aatattaaga ttaaatagta gatgagaaac
     8101 tcatgagcgt taccaacatt cataataaaa acgatagtgt acacgttaat gaattcgtgt
     8161 actactatcg ttttttattt ttatcgtgct tatcgttatt aaaataactg atacacaaca
     8221 cataaactat gaagaaaaaa ataaatccgc tatctaaatg actttgactc agttgtttat
     8281 atgaccaaat tgctaataca attcccatta ttattgaaat aacgtatctc acattcttat
     8341 acctataatc cttttctaaa aatatggttg ctattactta atttttaaag ttataaataa
     8401 aaagagccaa ccgcaatgga tggcccttgt tcattatgaa gcattagaac atttctgaaa
     8461 caaccttttg ttctaagaag tgtaataagt agtctgggct acctgtttta gcgtccgtac
     8521 ctgacatttt gaaaccacca aatggatggt atccaacaac tgctgaagta caacctctgt
     8581 taaggtataa attacctaca tcaaattcgt ttaccgcttt aatccaatgc tcgcgattat
     8641 ttgtaatcac tgcaccagtt aaaccgtaat ctgtatcatt tgcaacctca atggcttcat
     8701 caaaatcgtt aactttcaca aagccaacaa ctggaccaaa aatttcttct tgcatgattc
     8761 tatctttaga tttaagtcct gaaatgattg ttggttctac aaagtaacct tttgaatcat
     8821 cagtgccgcc accttgttct aatttacctt cttctttacc aatctcaata taatttttaa
     8881 tcttatcaaa ttgtttttta ttaataactg ggcccatata cgtattgtct acagtattgc
     8941 ccaacgttaa ttcttttgtt aatttgattg atttctctaa tacttcgtca taaacgtctt
     9001 tatgcacaat tgcacgtgaa catgcagaac atttttgacc agagaaacca aacgctgacg
     9061 ttacaatagc ttctgccgcc atatctgtat caatattttc atcaactaca atggcatctt
     9121 taccacccat ttcagcgata acacgtttca agaagttttg accttcttga actacggcac
     9181 tacgttcata aattctagta cctgtcgcac gtgatcctgt aaatgtaacg aaatgcgtat
     9241 ctttatgatc aactaagtaa tcaccaattt ctttcggatc accaggaaca aagttaacta
     9301 cgccttttgg taatcctgct tcttctaaaa tttccattaa tttataagcg atataaggtg
     9361 tatcctcagc aggtttcaat aacactgtat tacctgcaac aactggtgct aaagtcgtac
     9421 cagccataat cgcaaacggg aagttccacg gtggaattgt aacacctgta ccaattgatt
     9481 tatagaaata tttattgtgt tcaccttcac gatcaagtac tggcttacct tgagccaaat
     9541 ccatcattga acgtgcatag tattcaataa aatcaatacc ttcagctgca tcaccaactg
     9601 cttcatccca tggcttacct gcttcataaa ccataattgc tgcaatttcc gcttttcgac
     9661 gacgaataat tgccgaaaca cgtaacataa gctctgcacg atcatttgct gaccatgttt
     9721 tccaagattt ataagcttcg tttgctgctt taaatgcatc ttcaacatct tgttttgttg
     9781 cctttgatgc atttgcaatc acttgtgatg tgtctgcagg attgattgat ttaattttgt
     9841 catctttgaa aatcttctct ccattaatca ctaatggtat gtcttgacct aattcttttt
     9901 ccacgtcttt caatgctttc ttaaacatat ccacattttc ttggactgaa aaatcgtaac
     9961 caggttcatt tttaaattct actaccatgt acacttaccc cctataaatt ttgaaagtgg
    10021 tttaaccctt tgatttaatg atataacatc atttaaactc attttactat gattaaggtt
    10081 agttttgcaa tcgctttcat ttttatgctt tatcacttat tctcaagtat tttgaaattg
    10141 attggttact ttttaaaatt tatatgggtc gcaactgcta ctttatcgtt tcgtcattta
    10201 atgtttcgga tggtaggtca ttatcaattt tacgaacgac tttacaaggg tttccaaccg
    10261 ctaagctgtg tggcggaata tctttagtga caacactacc agcaccaatc acactgcctt
    10321 ctccaatcgt cacccctggt aacacggcta catgaccgcc aaaccaagta ttactgccaa
    10381 tatgaatggg tccggctttt tcataacctt catttctatg ataaaaatta agtggatgtg
    10441 ttgctgtgta gaatccacaa ttaggtccta taaaaacatt atcgccaatt gtaatctgtc
    10501 caccatccat aaaataacaa ttggtgttta catagacatt ttttcctagt ttaacgttcc
    10561 aaccataatc tgtatcaaaa ggaatcgaaa tacttacatt gtctgttgtt gtttgaaata
    10621 attgatcgat taattccttt cttttatttg tatcactcgg ctttgtatga tttaattcaa
    10681 agcaaatatc tttcgctcgt gcacgttcat tgattaagtc ttgatcaaag tttgcatcgt
    10741 accatttttc tgctaacatt ttttcttttt cagtcattac acctttcaac tcctaataac
    10801 ttatttactt gtttaaaagt taatcaaata aacattcgcc tatgcaacta atacgctata
    10861 acattatgaa atcatgacct tatcaccctt atctatacaa ttctcgcatc aaatactgct
    10921 aaagtagtag ataaattcaa tactacagac gcattcattt tttaatctat taacgtacaa
    10981 tgtgagtaag agaaatataa aggagtatga tagcgatgag aatattaatt acaggcacag
    11041 ttgctatctt aatcattcta ggtttagtta aaacgataca agattacgaa atgacaaacg
    11101 atacaagtcg tcagttgtca gacaacaaag atgatgataa agtcatccat cttaataatt
    11161 tcaaaaattt acatgcgaaa gaatttaacc catctgattt cttttaagtc acctaagaat
    11221 tgcaaatcca gaagtcattt aagttttacc tttcattcat acatccttta atattaatta
    11281 cgacttcttt tatatagatg ctaaatagag agattgttgt gcaatgtttg cacggcaatc
    11341 tctctttttc tttttaaaat tggtaaaagt aaaacgcaac gattgactta tatacctaga
    11401 gggggtacat tagacgtgta acaatgaatc acagggaggc aataatgtgg ctaatacgaa
    11461 aaaaacaaca ttagatatca ctggtatgac ttgtgccgca tgttcaaatc gtatcgaaaa
    11521 gaaactgaat aaacttgatg acgttaatgc ccaagtgaat ttaactacag agaaagcaac
    11581 tgttgagtat aaccctgatc aacatgatgt ccaagaattt attaatacga ttcaacattt
    11641 aggttacggt gtcactgtag aaactgtcga attagacatt acaggtatga cttgtgctgc
    11701 atgctcaagc cgtattgaaa aagtgttaaa taaaatgaac ggcgttcaaa atgcaacggt
    11761 caatttaaca acagagcaag ctaaagttga ctattatcct gaagaaacag atgctgataa
    11821 acttgtcact cgcattcaaa aattaggtta tgacgcgtct attaaagata acaataaaga
    11881 tcaaacgtca cgcaaagctg aagcgctaca acataaattg attaagctta tcatatcagc
    11941 agtattatct ttaccactat taatgttaat gtttgtgcat cttttcaata tgcatatacc
    12001 agcactattt acgaatccat ggttccaatt tattttagct acacctgtac aatttattat
    12061 tggatggcaa ttttatgtag gtgcttataa aaacttaaga aatggtggcg ccaatatgga
    12121 tgtacttgtt gctgttggta caagtgcagc atatttttac agtatttatg aaatggttcg
    12181 ttggctaaat ggctcaacaa cgcaaccgca tttatacttt gaaacaagcg ccgtactact
    12241 taccttaatc ttattcggta agtatttaga agctagagcg aagtctcaaa caaccaatgc
    12301 gcttggcgaa ttattaagtt tacaagctaa agaagcacgc attttaaaag atggtaatga
    12361 agtgatgatt cctctaaatg aagtacatgt tggagataca cttatcgtta aaccaggtga
    12421 aaagatacct gttgatggca aaattattaa aggtatgact gccatcgacg aatctatgtt
    12481 aacaggtgaa tctatccctg ttgagaagaa tgttgatgat actgtaattg gttcaacgat
    12541 gaacaaaaac ggtactatta ctatgacagc aacaaaagtt ggcggggaca ctgcgttggc
    12601 aaatattatt aaagttgtcg aagaagctca aagttctaaa gcgccgattc aacgattggc
    12661 agatattatt tctggttatt tcgttcctat cgttgttggt atcgcactat taatatttat
    12721 cgtgtggatt actttagtta caccaggtac atttgaacct gcacttgttg cgagtatttc
    12781 cgttcttgtc attgcttgtc catgcgcatt aggacttgct acaccaactt ctattatggt
    12841 aggtactggt cgcgctgctg aaaatggcat tttatttaaa ggtggcgagt ttgttgaacg
    12901 cacacatcaa attgatacca tcgttttaga taagacgggt accattacaa atggtcgtcc
    12961 agtcgtgaca gattatcatg gtgacaatca aacgctacaa ctgcttgcta ctgctgaaaa
    13021 agattctgaa cacccattgg cagaagccat tgtcaattat gcaaaagaaa agcaattaac
    13081 attaactgag acaacaacat ttaaagcagt acctggccat ggtattgaag caacgattga
    13141 tcatcaccat atattggttg gtaaccgtaa attaatggct gacaatgata ttagcttgcc
    13201 taagcatatt tctgatgatt taacacatta tgaacgagat ggtaaaactg ctatgctcat
    13261 cgctgttaat tattcattaa ctggtatcat cgcagtggca gatactgtca aagatcatgc
    13321 caaagatgct ataaaacaat tgcatgatat gggcattgaa gttgccatgt taactggcga
    13381 taataaaaac actgctcaag ccattgcaaa acaagtaggc atagatactg ttattgcaga
    13441 tattttacca gaagaaaaag ctgcacaaat tgcgaaacta cagcaacaag gtaagaaggt
    13501 tgcgatggtt ggtgacggtg taaatgatgc acctgcatta gttaaagctg atatcggtat
    13561 cgccattggt acaggtacag aagttgccat tgaagcagct gatattacta ttcttggtgg
    13621 cgacttgatg cttattccta aagccattta tgcaagtaaa gcaaccattc gtaatattcg
    13681 tcaaaatcta ttttgggcat tcggctataa tattgccggt atccctatag ctgcattggg
    13741 cttacttgcg ccatgggttg ctggtgctgc aatggcacta agttcagtaa gtgttgtcac
    13801 aaacgcactt agattgaaaa agatgcgatt agaaccacgc cgtaaagatg cctagattcc
    13861 ttaataatga aggattcgtt ggtgattctg agataggcta gtgattggct ctataatgtc
    13921 gcggtttaca gttgggtctt cgctccaact gcatatatag ttacactttt cgcttagcga
    13981 attagtgtat cttacctaat agcttcgcct attaggttcc atcattatta taaataataa
    14041 gtacactatg gtttacagtt ggatcttcgc tccaactgca taagagcctg agacataaat
    14101 caatgttcta tgctctacaa agttataatg gcagtagttg actgaacgaa aatgcgcttg
    14161 taacaagctt ttttcaattc tagtcaactt tgccggggtg ggacgacgaa ataaatttta
    14221 cgaaaatatc atttctgtcc cactccctaa aaggaggtga atgctatgtc acaagaaatt
    14281 ttaaatgttg aaggtatgag ctgtggtcac tgcaaaagtg ctgttgaatc tgcattaaat
    14341 aatattgacg gtgtcacttc agctgacgtt aaccttgaaa atggtcaagt aagtgttcaa
    14401 tatgatgaca gtaaagttgc tgtatctcaa atgaaagacg caattgaaga tcaaggttac
    14461 gatgtcgttt aattaggcaa tattcaacgt catcaacacc aaattaaaaa aatcgaactg
    14521 atgagaatcc caacaatcca aattatctca tcagttcgat ttttaattta ctcgtaacct
    14581 agtatctcca gtctgcaata catctaatgt tgcatctaat gcatcgacaa ttagattttt
    14641 aactgcagct tcagtataaa acgcaatatg tggtgttaat atgacatctt ccctgtcaat
    14701 caacgattct aacaatggat cgttcggtgt tttgcccctt tgatcacttg ggaaaagttt
    14761 gcgttcaaat tcatacgtat caagtgctgc acctttaatc acaccattgt ctaatgcgtc
    14821 taataacgcc ttagtatcta ctaaagaacc tctcgcacaa ttgacaaata ctgcgccctt
    14881 tttaaaatgt ttaaataatt cagcattaaa tagataatga ttatatttcg ttgcaggtac
    14941 atgtaatgtc acgatatcag caccttcaac cgcttcctca atcgtatctt tgtaatcgac
    15001 atacgttgca attttagcat taggaaacgg gtcgtatgcg accacatcac tttgataacc
    15061 attggcaaat atatcggcta ctacacggcc aattcgacct gtaccaataa cagctacttt
    15121 taaatcttta atggatttcg ataaaatagt aggttcccat ctaaaatcat gctcccgcac
    15181 tttcgtttga atttgattaa aatgacgaac cacattaata gcctggttca cagcaaactc
    15241 cgcaattgaa ttcggagagt atgacggcac atttgacaca atcaagttat acttgtttgc
    15301 taactccaaa tcatatgtat caaatccagc actacgttgt gcgatttgtt taatacctag
    15361 ttcatttaat cgtttataaa catgctctga taatggtatt tgttgtgata gcgataagcc
    15421 atcataacca gcgacacctt caacattgtc atcagttaat gcttctttag taatatctac
    15481 ctcaacatga tgtttctctg cccacgcctt gatataaggc atatcttcat cacgtacact
    15541 catgatttta atttttgtca ttttaacatc acccttaact ttattattca tataaatatg
    15601 ctagttctgt taatcttatt gcagcttcgt ctaatttctg gtcatctaac gccaatgaaa
    15661 ttctcacata acgattacca ttctctccaa atggtttccc tggagcaaca agtattgact
    15721 tctcttgcac taaaaattgc tcaaattgct cgctgtcata accaggcggt gtttccaacc
    15781 atacatatat gccaccttta gcatgaacaa atggcaaatc agcttttgca agcatggctt
    15841 cgaatcggtc acgacgtgtt ttaaatacat tgctttgttc ttctaaaaaa tcatcataat
    15901 gattcaaagc atatattgcg gcatcttgta atgcaccaaa catcccagca tttgtgtgcg
    15961 tttggtactt tttcaaagct tgaatcatat ctttattacc aactgcaaaa ccgactctaa
    16021 aacctgacat gttataacct ttagacaagg aataaatttc aatcgcaaca tctttcccat
    16081 tttccgaagc aagtatacta ggatttttag cgtcgaaacc gaaagcacca taagcaaaat
    16141 catgcacgat tttagtgtct gtacctttaa atttagctat cgcttcatca aaaacttctt
    16201 tcgtagctgt cgatccagtt ggattatttg gatacgttaa ataaatgagt tttgttttat
    16261 ctattatttg tgaatcaact gtggaccaat ctggcaaata atgtggcggt tctaaattaa
    16321 gcggaactgg cttgccatca gctaaaagta cacctgctaa atagtccgtg tagcctggat
    16381 caggtagtaa tacatagtct cctggattga taacacatgt tggtactgcc actaatccat
    16441 tttttgtacc atataaaatg catacttcat cttctttatc taacgtcaca ttatattgtc
    16501 tttgataaaa atctacaata gcttgcttga acgcttcttt accatgaaaa gcaccatatt
    16561 tttgattttc aggaatagtt agtgcttttt gaaaatgatc aataatacct tgtggcgtgg
    16621 gcccatcagg gatcccaact gccatattaa ttaatggcaa tggtccatgt tcgattttac
    16681 gtcccatcgt tttcccgaaa taactatcag ggatatttgc taatttgtta gagatcatca
    16741 aattcctcct ctatcattaa acatagcctg ggcgactatc ataatcctaa caacttgtat
    16801 cactctcatt tagatggtta caatgacatc gccattcacc gttatgttca acagaactta
    16861 tgacacacgt tgtattgaat gaatttattt tcattttagg taggtacatt attattgtca
    16921 atattaggaa ttttcagatt aatatgcact caatcgttat gatttaactg tcatgcatat
    16981 ccgcatgcgc aaccagttag atatgcttat ataaagtata acgcccctca aggtacgtat
    17041 tcaaacgtga accttaacag gcgcctattc attgttaaat aaaacttctt tagaacatac
    17101 ttatttcact atgcctttta ctttcccctt atacttttct cacatctttc tcttagacta
    17161 ctcccttata cgccccgctc aatatcttta atcatttcgt ctacagttat tttcgcactt
    17221 gttaagacaa taggaacgcc tgcacctggg tgcgtacttg cacctgcaaa atataaatct
    17281 ttataatctc gcgatacatt ttgtggacga taataattac tttgcgctaa agttggcatt
    17341 aaaccgaatg ccgaaccaaa tttcgcatga tacgtttgct caaaatcatt tggcgtaaag
    17401 attgtttctg aaacaatatg cgattttata tcttcaaata cttcaattgt tgctaattta
    17461 cgataaataa tttcctttat ttgttgcgtc aaagcttcat ctgaccaatc gattccgcta
    17521 cctgttttaa gttccggcgt cggcattagc acataaatac cagttttgcc ttctggcgca
    17581 agtgatttat cagcgaccgc tggtacatac acataaatag aaggatcata tgataaacgt
    17641 ccctcaaata tttcttcaat attgcctcta aagtcatctg aaaaaataac attatgaagt
    17701 ctcacttgat ctgtcacatc aatatctata ccgatataca ttaaaaatgc tgaacaagag
    17761 taatctaagt ctgcaatttt atgtggtgga tactttttaa taggtgcaaa atctggcatt
    17821 aatgattccg caacactagg gaaatcagcc gtacataaaa ttttatcaaa ttttcttatg
    17881 tcaccattca cttttatcgc atcggcccgt ttgaatttag gatcaataat aatttgctca
    17941 atttcagcat ttagttcaat attaacgcct aagtctttat ttaattgcgc tagcccttga
    18001 gccataccat acataccgcc tttaataaaa tgcacaccaa acatcatttc aatcatagga
    18061 ataattgaat atagtgacgg gcctcgtttt ggatcaattc ctatgtataa cgtttgaaac
    18121 gctaaaagct tttgtatctt ttcgttatca atataatgtt caattagctg atctgcatga
    18181 tttaacgttt ttaacttagc accttgcaca agtgacgtca tattataaaa gtcactcggt
    18241 ttgcgatacg ttctttctaa gaaatagcga cgtgcaattt catatttttt ataaacatcc
    18301 gttaaaaagg acataaaacc atgcgttgaa ccaggttcta tactttctag catttgctgt
    18361 aattcagcta aatctgtagg caccgttata cgatcatcgt ggtcaaaata cacatcgtaa
    18421 atataacgta attgtctcaa ttcaatataa tcttcataat ttttaccaca cgctgtaaaa
    18481 acatctttat aaacatctgg catcatgaca attgtgggac ccatatcaaa tgtaaagccg
    18541 tctttcttta attgattcat acgcccgcct acattattat ttttttcaaa tatcgtcact
    18601 tcatgacctt gagaagcaat acgggctgcc gctgctaatc ctgtgacacc tgcaccaatt
    18661 actgcaatct tcattattca accacctata ttctatgata tttactattt atttcatgaa
    18721 acaactttgc ctttttccgc ttatccacaa aaacacgttc atgtaatgta tagttagcct
    18781 gtctcacttc gtccagtatt tcaatatata tacgtgctgc taattctatg attggttgtg
    18841 cttcaatact aaatactttg atttgatcca taacatcttg aaaatctttt tctgcgatag
    18901 ctgcataata ttcccataag tcaatataat gattattaac accattttgg tacacttcag
    18961 caatatcaac ttcatattgc tttaatcgtt gcttactaaa atatacccgt tcattgtcaa
    19021 aatcttcacc gacatctctt aatatattaa tcaattgcaa cgattcacca agtcttcttg
    19081 cgacatcgta tgtctgatgt gtttcatgat cacttaaaat cggcgtcaat acttcaccta
    19141 ctgtaccagc aacaccataa caatatccga ataattcagc gtccgtttca aacattgtaa
    19201 aatgttgatc tttatataca gtatcaatga gattataaaa agattgaaag gcgatatttt
    19261 tatgttgtgc aacatgctga agcgccatca tgattctacg atcactttga aagtgatgat
    19321 gttcatatgg gtatttttca atagattgta tatcttcttt tatttgattt aaaaattgaa
    19381 tatcgccata aacatctata ctgtcatcaa ttttacgaca cacagcataa attgcccaaa
    19441 ccgcttttct ttgatcttct ggtaacaagt caaaagcgta agaaaagctt tttgaatgtt
    19501 tcttcatgat tttatgacaa tatttaaaat tcatatccat cattgtcata ctagtcctcc
    19561 tatattgaaa tgccattacc ttgatttatt gttctttaga tatagaatat tgacgacctt
    19621 tccattcaac tacaccataa cgatgcgttt gtttccaaga ttggatgaaa attttagtaa
    19681 aaaacataaa taacaatgga tgacatacca ttaataaatt agaaaattgg ccgacccttc
    19741 gatgcagata aataaattgt gtagtatagc tcaagtaaag tgctaccatt tttcttacag
    19801 acatttggcg atattttaaa ctaagacata gccctaaaat actcgctata gaaccgaaca
    19861 accacaacac aattgctgtc atgatcttag gctttgtgcc acctgcccca gttgacaaat
    19921 gctttgtcca tccttcttgt aatgactgaa atccttcttg atacatgcga aatgcaacaa
    19981 atggaaatcc ttcataaact gttacgggca atgattgtga agtatatgca cttcctaaag
    20041 caaatccttc aataatatga cggttcgcac ttttatgacc tccagttgca taataatctt
    20101 ctttatttgt taatgtcacc ggtccaaatg catgctggtt agtccgaccg tcgtctaagg
    20161 tagaaaatac attcatacca acgactgtca ttaaattaaa tatcgctgaa aacccttcgt
    20221 agaaacgctt tgttatatga taaggctgta cgcttaacaa tcctttttca ccttgtaatt
    20281 gatactgatt aatcaacgtt tcaacagcat ctttccttaa gaaagttaca tcagcatcta
    20341 caaaggcaat acgattcgta catgcatgcg tcacaccttg ataacaagca tgtgatttcc
    20401 cataccattt gccgtcagta tcatcaacaa catctactac agtcgcacca tatgaacgtg
    20461 ctacacgtgc tgtttcatct gtcgatccgt cattcataac aatgacatct actggaactt
    20521 gctgttgtat tatcgaatgt agtaaatgac ctattctttt ttcttcattt ctagccggaa
    20581 taataattgt taatgcttta tgattgaagt tcagcgtttt cgtctttagc tgatgtctac
    20641 gattaaatat caatgcacca cacgccatag acatggtcac tattactgtt aatattcgtg
    20701 ataaccattt catagatatc acttcctatt cttcgcttct cgcgcgttta ttttgtctgc
    20761 gacttgttgc ccacttaatg taaccattgg catgccacca ccaggattta ctgatccacc
    20821 tacaaagtac aagttttcaa aatactgact ttctttagga aatttaaatc ctttgttttt
    20881 ctttttatct gctacaacac catatattgc accacgatta gaacgataat ttttttcaat
    20941 atcctccggt gtccaaacat cttcataaat aatgtgtttt cttaaatcag taagtcccat
    21001 tttttctaat ttatccaaaa ttttatccct aaacttcgca taatcttcag tggtaaaagg
    21061 ctgatcttga atatatggaa tatgtggtaa gactttgata ttttcataac ctactggcgc
    21121 ttgtgtgtga tcagttttat tcgtatttac tagataaatg gtcggatcat ctggcaatac
    21181 cttttcatga aaaacttgtt gataattgag ataagcattt tccgtaaaaa agaaattatg
    21241 atgtgctaat tgcgggtatt ggcaagcaac acctaaatgc atcacatatc ctgagcttgc
    21301 cggctcaaat tccctctcta atttgtttaa tcgttgagta tcaaggtgaa ttaaatattt
    21361 ataagtaggt atgacttcca tatttgaaat aatataatct gcctttacaa actcacctgt
    21421 atctaatctg acacccgtca cacgtctttg atatgtttta atattgtcca cacgtgcacc
    21481 tgtatgaatt gtgacacctt cttcacgcgc tagcttttcc aaggcattgg ctaaatgatg
    21541 gattccacct tctacatacc aaaggccttg ctcttgttgc atatggaata acatagataa
    21601 tacagctggc gcatcgtatg atgaagaacc tacatatttg ataaaatagc ctaacatttg
    21661 tcgcaagtat ggattcgaga tacgcttgtc tatggcctgt tgcatagtat gtacataatc
    21721 ataattaata agagcattta atggcccatg aaatttaata atttgagata gtgtatctaa
    21781 accatagtta aaataccctt tttcagtaat acgatcgatt cgtcttgtat aattcaaata
    21841 attttgcagt tcctctatat cctgtttcga caatatcgca ttatgctgac ctgtttcttt
    21901 aataccttca tacaaatcga tagtcgtgcc atctggaaaa aagctacgcc attgatgtgg
    21961 caaacgcttg attgtaacgt agtctgacat ttgcttcttg ctatattcga ataatttttc
    22021 aaaaatataa ggcatcgtta aaatagatgg acctaaatca aagccaaagc catctgattc
    22081 atgacgattc actttgcctc ctatatgagt attttgttca tataatgaga ccgaatagcc
    22141 actttgtgcc attcgaattg ctgcagaaat cccacctaag ccaccaccaa taacgatgat
    22201 atgtttagtc atgacgttca cctcttcgtt ttaatatgcg tagtaactgc gttaatctcg
    22261 gtcgattata gcgttgcaca ataatgattg gaacattagc tatcattgca tagaaaatat
    22321 ttatatactt tactaaacga gagcctttat tgaatatgat gactggaagt atcgatatcc
    22381 aatgaatcaa ctctgccctc tttgtctcta taatcatttt ttcaatttca tcagtattga
    22441 ttgtcattaa atgacgctga tcatatatat tttgattaag ctgatgacca tctaaaatct
    22501 tatgtttcca ttttcttacg taaaaccatt tgttccaaaa ttttccatgc ttctcaaaat
    22561 taaatgattt aaatattatg tacttctgac gaaaatactt atcaggaatt cttgtaccta
    22621 aatttgctat atttagttga acaattaacc aatacatact acaaaaaaat gctgttttga
    22681 tatatttttt catggttttc atctaaattg aatcactctc aatcatactg acatagtata
    22741 aattatcact ttattactta gttccctaaa gatcagtgaa taaaccgctc tcatttatat
    22801 ttatataaaa caaaaacgcc aatactctca tgttagagaa tattgacgtc atttgattta
    22861 catttgatta atgaatgaaa ttatatgaac ctgcttggtt tgctgaaatt gtacgagacg
    22921 taaccacacc agcaccatgt ccatagttca tttctgaaac acgaacagaa ccgttgctgt
    22981 taacgccttc aacgtaagca acatgaccgt aatagccttg tgttgtttgc atgatagcac
    23041 caacttttgg tgtattgttc actgtatagc cagatgatgc agctgcgtta gcccaattac
    23101 ttgcgttacc ccatgttgaa ccaattttcc caccaacacg atcaaataca taataagtac
    23161 attgtcctga agtatataag ttacttcctg atgcataacc attagaaatt gaacgaccat
    23221 ttgaagatgg cgctgcagtt gtagtcacat gaacattatt acttgttgtg ctataacttg
    23281 cacctgaacc accagtataa tagttatttg ttgctgtttg gctattgttt gtatagttat
    23341 tgtattgata tgaatgatta tagtagttat tgtaagatgc attgttataa ctataagtat
    23401 tgtagttgtt gtagtagtat gtgttgtttt gcgttaattg acttggattc caatttcctg
    23461 tccaagtgta atgataatta ccttgtgcat caattgtata cgtatagctg tatgattgag
    23521 cgtcattaga attatatcca ttgttatttt gttctgcggc ttgtgcatca tgtcctgcaa
    23581 atgcgatagt ggcaagtcct gctgtagcga ttgtagctgt aacgattttt ttcataatta
    23641 aatatcctcc caataatcaa aacactctat acatatagtc attagtgact caattatttt
    23701 aaattttata ttttttattt ttttagtatg caaataatta taacttttat agttttgcga
    23761 caaaacaaac tataacagag attaggtgtc ttgcgggctt tgttatcata atgtaatctg
    23821 ttcctaataa agcttcattg aatgtaatat accaattaga atgtttacaa cactttttct
    23881 gcaccgttaa catgggattt gaaaataaaa aagcactaaa cttttaatga aatacatcat
    23941 ttttgataat aaatatcaga agtaatgtaa tatttcaaaa gtttagtgca tcaagttatt
    24001 tcttatttgt agcatgtgtt tccatcgttt ttacaatcaa atcagtcagc gctttactac
    24061 ctgcatattc taagtgaata ccgtcatatg caaagtattc cggatgacct gcagaagctt
    24121 tataccaatc gactagatgt acattagagc gcttttcagc tgcctcataa attaatttat
    24181 ttattctacc ttcataatct ctaggtactc taatagaaac taaatatatg tctgcttttc
    24241 caaaactatc caatagttca tttaattgat ctttcgtaaa tgccccattt gtaccaagct
    24301 ctactacaac tttttgacct tttttagcat agtctttata ttgcgatttc acaattggtg
    24361 tagcatcaac gagttgccgt ccaactttac catcaatttg tgcatttggt attttcttag
    24421 taaagacatt accaatatcc accatgaccg agtcaccaat taatagtggt gatgactttt
    24481 taatatcctc tttgctattc gctgtctgct tatcctcttg cttatctttt ttaacgactt
    24541 ttttcttcgt tgttttaaat tccgtcttct tctctttctt ctcggcttca tgttgtttgc
    24601 caagtgcatc aaactgtcca ctgagcacaa cgatagacgg aaccaataat aggataacta
    24661 acactgttct agcaaattgc cccttctttt taggtaaaaa tgcaaaagct ttaaatcctt
    24721 ttttacgtat aggtgtttca ataaagcgat acgaaatttc agccattaac gctgttaaca
    24781 aaatttctat aatataaacg tataccggta tttgtccttg tacgtaataa ctgttcacaa
    24841 aaacaatgat aggataatgc cataaatata agctatatga tcgtttacct ataattagta
    24901 aaggtttcat acttaaaaat ttagcaaata aactagaagg atgtaccgca attgcaataa
    24961 tgaataaagt tgcaaatgat ataatgtaaa atcctccatt atagatccat tgatcttggt
    25021 cgccaactat aaagaacaaa gtcattagaa ccgcaaaacc agatatccct ataatatcta
    25081 atgatacgac aatcttttta gaaatatctt ttttcaaagc aaacggaggc caaataaatg
    25141 ctaatataca accaagcaat aaagtttgca gtcgtgtatc tgtcccaaaa tacacacgtg
    25201 aattatctcc agtgatgaaa tgaatcacta tcataagtcc taaagaaatc aacgatacaa
    25261 taaatagcgt ttgaataata tttctcggtt taaatctatg taataagaac gtgataacca
    25321 atgggaaaag caagtaaaat tgttcttcaa tggctaaaga ccataaatgt ttgagtggtt
    25381 caatagcaaa ttggttaaaa taatctacat tctgtgaaat gtaccaccag tttgaaacat
    25441 agaatatagc tgcaatagca tctcgtttca tttgtataat caattccggt ttaaatatca
    25501 gtgtgaacgt aagcacgaca cagattaaaa ataacactgc cggaatgagt cgtttcaatc
    25561 gtcgcttcca aaactctagc aaatcgattt tttgcgtccg ataatactca cttatcaaca
    25621 aacttgttat taaataacct gaaataacga agaatgtatc tactcctaaa aagcccccac
    25681 ttaaccattg tgcattcaag tgataaataa tgattcctat aactgcgaat gccctcaatc
    25741 catctaatcc gggtaagtat cgcggggaat acattttttc taaacgttta aagtcttttg
    25801 tatccatgtt aataaacgcc ccatttattt ttctctattt tgtagcatat cacaatattt
    25861 ttgaaaataa aatattgcac tgattttcat taattgattt aacccttaat taagatagtt
    25921 ttaaattttt tattaagtag aaaacaatta ttacagttaa tttcattact gcaaaccaca
    25981 tataaatttg tcgattttac tacataacat agattatcat agattcttga atttttagca
    26041 aaataactgt tattttcatt atatttttac aaaaaaaggt tcgttttata ttttatgcat
    26101 cttactgtaa cagaatcatt aagatatgct attcgaattt actttttcaa aatttctata
    26161 atgaataaat taccatgtat tgaaaataaa gcgaaatgca gcctatcctc taatttaaac
    26221 caagcgatat atcacgtcag cctttatatt taaacgctat gtgtcacttt taaaatgaat
    26281 attactaaga ttgtcatacc aattattcat tgcatcgaat taatctttta aatttctgta
    26341 ataaaaccta ttatctacat ctaatttagg aaaactactt ggtaaatctc ctcgctt
//
LOCUS       DAHHSC010000016        25762 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.016, whole genome shotgun sequence.
ACCESSION   DAHHSC010000016 DAHHSC010000000
VERSION     DAHHSC010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 25762)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25762)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25762
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.016"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            <1..612
                     /gene="ccsA"
                     /locus_tag="NBI57_000525"
     CDS             <1..612
                     /gene="ccsA"
                     /locus_tag="NBI57_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HCD1821501.1"
                     /translation="ISDVFYTLSWLIISISLILNLIKVLNFSVFFLNLIGLTLLGMNT
                     FQPTHYTNKVQKIAVVDELLLVHIGLAVLSYVFFALAFVNALLYIMQYRNLKEKRFDQ
                     KYFRIGSVATLESIVFYSTLSGWIILIFSIILGTQWGVISVGERIFIDPKVILSSIIT
                     VLYGSYILLRINKWLNSRYLIYYNIILFCLNMINLFFATHFVN"
     gene            654..1580
                     /gene="hemC"
                     /locus_tag="NBI57_000526"
     CDS             654..1580
                     /gene="hemC"
                     /locus_tag="NBI57_000526"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HCD1821502.1"
                     /translation="MRKLVVGSRRSKLALTQSQQFINKLKAVEPNLEIEIKEIVTKGD
                     RIVDKQLSKVGGKGLFVKEIQHELFEKNIDMAIHSLKDVPSVIPEGLTLGCIPDRELP
                     FDAYISKTHTPLSQLPEGSIIGTSSLRRGAQILSKYPNLEIKWIRGNIDTRLEKLQTE
                     DYDAIILAAAGLRRMGWSDDIVTSYLDRDTLLPAIGQGALGIECRSDDEELLTLLSKV
                     HNDEVAKCVTAERTFLAEMDGSCQVPIAGYATISDQKEIEFTGLIMTPDGKERFEYTM
                     NGTDPVELGKTVSNKLKEQGAYEIIKRLNEQH"
     gene            1602..2270
                     /locus_tag="NBI57_000527"
     CDS             1602..2270
                     /locus_tag="NBI57_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HCD1821503.1"
                     /translation="MKPVVVMTQTNDMQSDLVSIIHKPFIDIKPLNFDIHLLNQRYDW
                     LIFSSKNAVKFFYKYLKGINVDNIAVIGSKTAQYCESLGIRVDFMPNDFSQEGFLKSF
                     NQTNQKILLPSSELARPLLLAALSKDNEVVKIDLYTSVPNKQNIQDVKEMIEHQQIDA
                     LTFSSSSAVRYYFNEGFVPKFKSYFAIGEQTARTIKTYQQPVTIAEIQTLESLIEKIL
                     ESRG"
     gene            2273..3247
                     /gene="hemB"
                     /locus_tag="NBI57_000528"
     CDS             2273..3247
                     /gene="hemB"
                     /locus_tag="NBI57_000528"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001633925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HCD1821504.1"
                     /translation="MKFDRHRRLRSSATMRDMVRENHVRKEDLIYPIFVVEKDDVKKE
                     IKSLPGVYQISLNLLESELKEAYDLGIRAIMFFGVPNSKDDIGTGAYIHDGVIQQATR
                     IAKKMYDDLLIVADTCLCEYTDHGHCGVIDDHTHDVDNDKSLPLLVKTAISQVEAGAD
                     IIAPSNMMDGFVAEIRRGLDEAGYYNIPIMSYGVKYASSFFGPFRDAADSAPSFGDRK
                     TYQMDPANRLEALRELESDLKEGCDMMIVKPALSYLDIVRDVKNHTNVPVVAYNVSGE
                     YSMTKAAAQNGWIDEERVVMEQMVSMKRAGADMIITYFAKDICRYLDK"
     gene            3295..4581
                     /gene="hemL"
                     /locus_tag="NBI57_000529"
     CDS             3295..4581
                     /gene="hemL"
                     /locus_tag="NBI57_000529"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HCD1821505.1"
                     /translation="MRYTKSEEAMKVAETLMPGGVNSPVRAFKSVDTPAIFMDHGKGS
                     KIYDIDGNEYIDYVLSWGPLILGHRDPQVISHLHEAIDKGTSFGASTLLENKLAQLVI
                     DRVPSIEKVRMVSSGTEATLDTLRLARGYTGRNKIVKFEGCYHGHSDSLLIKAGSGVA
                     TLGLPDSPGVPEGIAKNTITVPYNDLDALKIAFEKFGDDIAGVIVEPVAGNMGVVPPI
                     EGFLQGLRDITTEYGALLIFDEVMTGFRVGYHCAQGYFGVTPDLTCLGKVIGGGLPVG
                     AFGGKKEIMDHIAPLGNIYQAGTLSGNPLAMTSGYETLSQLTPETYEYFNMLGDILED
                     GLKRVFAKHNVPITVNRAGSMIGYFLNEGPVTNFEQANKSDLKLFAEMYREMAKEGVF
                     LPPSQFEGTFLSTAHTKEDIEKTIQAFDTALSRIVK"
     gene            4642..4743
                     /locus_tag="NBI57_000530"
     CDS             4642..4743
                     /locus_tag="NBI57_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1821506.1"
                     /translation="MGGDLIVKVHICFVVKTASGYCYLNKREAQAAI"
     gene            4777..5850
                     /locus_tag="NBI57_000531"
     CDS             4777..5850
                     /locus_tag="NBI57_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HCD1821507.1"
                     /translation="MTMIYRNNFIVFVLSFFISIILYSSHVLLPFMFGPIIASIICVK
                     VFKLDIKWPFLLSELGIVLLGVQIGSTFTKNVVMDIKNNWLSIIVVSISILLIAIVMA
                     FIFKKIARINTETAILSVIPGALTQMLVMAEQDKRANLLVVSLTQTSRIIFVVVLVPF
                     ISYFFHDGNMHANGKLTKVLPLSQVLNIGQIVILAIAIFIVYLIMSKIKFPTFQLLAP
                     LIVLIVWNFSTGLTFTLDHWLLNMAQLIYMIRIGVQIAHLLSDLKGRLAIAITIQNIM
                     LIIGALIMVYIIHFFDNNPINELFLGAAPGGVNQIVLVALATGADVAMITSYHIFRIF
                     FILFIIAPGINYFLKYRSNKRED"
     gene            complement(6073..6633)
                     /locus_tag="NBI57_000532"
     CDS             complement(6073..6633)
                     /locus_tag="NBI57_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HCD1821508.1"
                     /translation="MNECAFGTKDPVYLNYHDHVWGQPLYDSKALFKLLALESQHAGL
                     SWLTILKKKEAYEEAFYDFEPEKVAQMTAQDIDRLMTFPNIVHHRKKLEAIVNQAQGY
                     LKIEQAYGSFSKFLWSYVNGKPKDLQYEHASDRITVDDTATQLSKDLKQYGFKFLGPV
                     TVFSFLEAAGLYDAHLKDCPSKPKHN"
     misc_binding    6691..6992
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            7036..9666
                     /locus_tag="NBI57_000533"
     CDS             7036..9666
                     /locus_tag="NBI57_000533"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HCD1821509.1"
                     /translation="MEMKPKYDPREVEAGRYEEWVKNGYFKPSEDKSKETYTIVIPPP
                     NVTGKLHLGHAWDTTLQDIITRMKRMQGYDTLYLPGMDHAGIATQAKVEAKLNEQGIT
                     RYDLGREKFLEQAWDWKEEYASFIRAQWAKLGLGLDYSRERFTLDEGLSKAVKKVFVD
                     LYNKGIIYRGERIINWDPKARTALSDIEVIHEDVQGAFYHFKYPYADGEGFIEIATTR
                     PETMLGDTAIVVNPNDERYKDVIGKTVILPIVGRELPILADEYVDIDFGSGAMKVTPA
                     HDPNDFEIGQRHQLENIIVMDENGKMNDKAGKYEGMDRFDCRKQLVKDLKEQDLVIKI
                     EDHVHSVGHSERSGAVVEPYLSTQWFVRMEDLAKRSLDNQKTDDRIDFYPQRFEHTFN
                     QWMENIRDWTISRQLWWGHQIPAWYHKETGEIYVGEEAPTDIENWQQDEDVLDTWFSS
                     ALWPFSTLGWPDLESEDFKRYYPTNALVTGYDIIFFWVARMIFQGLEFTDRRPFNDVL
                     LHGLVRAEDGRKMSKSLGNGVDPMDVIDEYGADSLRYFLATGSSPGHDLRYSTEKVES
                     VWNFINKIWNGARFSLMNIGEDFKVEDIDLSGNLSLADKWILTRLNETIATVTDLSDK
                     YEFGEVGRALYNFIWDDFCDWYIEMSKIPMNSNDEEQKQVTRSVLSYTLDNIMRMLHP
                     FMPFVTEKIWQSLPHEGDTIVKASWPEVRESLIFEESKQTMQQLVEIIKSVRQSRVEV
                     NTPLSKEIPILIQAKDKEIETTLSQNKDYLIKFCNPSTLNISTDVEIPEKAMTSVVIA
                     GKVVLPLEGLIDMDKEISRLEKELAKLQSELDRVDKKLSNENFVSKAPEKVINEEKRK
                     KQDYQEKYDGVKARIEQLKA"
     gene            9679..10950
                     /locus_tag="NBI57_000534"
     CDS             9679..10950
                     /locus_tag="NBI57_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HCD1821510.1"
                     /translation="MNYLESLYWIHERTKFGIKPGVKRMEWMLAQFNNPQNNIKGIHV
                     GGTNGKGSTVAYLRTALVENGYEVGTFTSPFIETFNERISLNGVPISNDAIVELVSRI
                     KPVSEMMERETDLGVATEFEIITAMMFLYFGEIHPVDFVIVEAGLGIKNDSTNVFTPV
                     LSILTSIGLDHTDILGGTYLDIARDKGAIIKPNVPVIYAVKNEDALKYVRERAIEQHA
                     KPIELDREIVVVSQNDEFTYRYKDYELETIILSMLGEHQKQNAALAITALIELNEQGL
                     IELDFNKMIDGIESVRWTGRIEQVHDKPLIILDGAHNSESIDALIDTIKQYHDKEKVD
                     ILFSAINGKPINEMVKHLSLIAHTFYATEFDFPKALRKEEIVGSIENDEIQLVDDYVE
                     FIKNYQGDTLVITGSLYFISEVKSKLDFEAL"
     gene            11220..11927
                     /locus_tag="NBI57_000535"
     CDS             11220..11927
                     /locus_tag="NBI57_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HCD1821511.1"
                     /translation="MVVLLSYSCSCIFSFLYQFISIEETSFDYLHRRSKCDYCNSSLK
                     WYELMPIISFLLLKGRCRNCRKRISLTHFLGETFALIPIVFIKYDFTYVNATLFITTY
                     VFLLIFTMTDITSLMLDCRLIIIYCIVSLSLSMIYPVAFIIISMTTHIFYFLFRAYIG
                     YGDVLLISALSLFFPLQFTIYVILFTFVIAGLVALITMIFKPIKLLPLVPFIFISFFI
                     NSLFYNDIHQFLGGVYF"
     gene            11924..12610
                     /gene="radC"
                     /locus_tag="NBI57_000536"
     CDS             11924..12610
                     /gene="radC"
                     /locus_tag="NBI57_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000692885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HCD1821512.1"
                     /translation="MKIKEMVTSEMPRERLLSHGAKSLSNTELLAILINTGRKGFSSI
                     DISNELLKSASNLNELKKSSINDLIKVKGIGLQKAITLKAAFELGERMGRRAENNRIK
                     ITQPSDVADYMIPTMKDLTQEHFVILLLNSKNVVIKETCVFKGTLNSSIVHPREIFSI
                     AVRENANAIIAVHNHPSGDVTPSQEDIITTMRLKECGLILGIDLLEHIIIGDNRFTSL
                     VEAGYFDEND"
     gene            12678..12962
                     /locus_tag="NBI57_000537"
     CDS             12678..12962
                     /locus_tag="NBI57_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1821513.1"
                     /translation="MTLFIIIGVLVPMVYTMQLNIKNEPVTKRNLLITLALSTLGILV
                     TALAGVIVTKQAFPLLSVAIGSIFTGIVWGLLLSGSYALIRFLSNAFGRK"
     gene            complement(13079..13276)
                     /locus_tag="NBI57_000538"
     CDS             complement(13079..13276)
                     /locus_tag="NBI57_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008530242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1821514.1"
                     /translation="MSILTIILIALLVILLFRVGLSILRFLIYVGLVLLCIYLGYQGL
                     IWLLDFFQINSGFLPHFQFNN"
     gene            13436..13909
                     /locus_tag="NBI57_000539"
     CDS             13436..13909
                     /locus_tag="NBI57_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4930 family protein"
                     /protein_id="HCD1821515.1"
                     /translation="MRLIFSMIKNIIAVIAIVIIVYIALKYAPFLKEQDWNPVNHDND
                     QMNQVTQPTNDAQHVYVSGEKYSLKENDLIKNVPLSQIKNVFKMIDKQEFMAVSGMNR
                     MAYNDQYIIGQRGDEFILYKFGDESMRVYNTEFEMQQDLNELGQNLQLKPENAYQ"
     gene            13983..14102
                     /locus_tag="NBI57_000540"
     CDS             13983..14102
                     /locus_tag="NBI57_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1821516.1"
                     /translation="MAEQSKQKQANEQQKAQNLFARWRQLQNSNSESSNDTNK"
     gene            14301..15143
                     /gene="mreC"
                     /locus_tag="NBI57_000541"
     CDS             14301..15143
                     /gene="mreC"
                     /locus_tag="NBI57_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HCD1821517.1"
                     /translation="MLKFFKNNKLIVVLCAIIVFIALIGLSIRSQSQSPPEQYIGDSV
                     SFGQRVVSYPVNFVAGTIGDFFKKGDSKESKNKISQLESKNQQLEAENEKLKKELDLK
                     DISKFDPISTTVLARNPDQWMNTIVIDKGSKSGITSNMAVMTSQGFVGRVTKVNKFSS
                     QVDLISTNTRAGKLSVNIQHGSKNIFGLIDRYDEKNSELVISDINNRDNISKGDKVVT
                     SGLADQLPSNLYIGEVTKVQNDQYGLAKEVRVKTGADLTDLSHVYVAKRDPKTIPDDE
                     SRDK"
     gene            15143..15673
                     /gene="mreD"
                     /locus_tag="NBI57_000542"
     CDS             15143..15673
                     /gene="mreD"
                     /locus_tag="NBI57_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HCD1821518.1"
                     /translation="MRTLYYFLIGILLFYIDTAIGLLIPMHIGKFELVFVPHLTFMYI
                     LMMVVYRGFGVSLLLSIFLGVMTDVYFGSIYGVYLFGYILFLALIDRFFKIFYKDHSM
                     LFIIILASTLLLEVYVALIYGMLGFIQFDIIHFVVFRLLPTLIMNFVLLIMLYPLIIK
                     FLKKTNNDIDMKRRQW"
     misc_feature    15700..15896
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00559"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L21 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00559"
     gene            15909..16217
                     /gene="rplU"
                     /locus_tag="NBI57_000543"
     CDS             15909..16217
                     /gene="rplU"
                     /locus_tag="NBI57_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000457386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HCD1821519.1"
                     /translation="MFAIIETGGKQIKVEEGQEIFVEKLDVNEGDTFTFDKVLFVGGD
                     SVKVGAPTVEGATVTATVNKQGRGKKITVFTYKRRKNSKRKKGHRQPYTKLTIDKINA
                     "
     gene            16223..16543
                     /locus_tag="NBI57_000544"
     CDS             16223..16543
                     /locus_tag="NBI57_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000633692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HCD1821520.1"
                     /translation="MITVDITVNDEGKVTDVIMDGHADHGEYGHDIVCAGASAVLFGS
                     VNAIIGLTSERPDINYDDNGGHFHIRSVDTNNDEAQLILQTMLVSLQTIEEEYNENIR
                     LNYK"
     gene            16555..16839
                     /gene="rpmA"
                     /locus_tag="NBI57_000545"
     CDS             16555..16839
                     /gene="rpmA"
                     /locus_tag="NBI57_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009382703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HCD1821521.1"
                     /translation="MLKLNLQFFASKKGVSSTKNGRDSESKRLGAKRADGQFVTGGSI
                     LYRQRGTKIYPGENVGRGGDDTLFAKIDGVVKFERKGRDKKQVSVYAVAE"
     gene            17193..18485
                     /gene="obgE"
                     /locus_tag="NBI57_000546"
     CDS             17193..18485
                     /gene="obgE"
                     /locus_tag="NBI57_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020444729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HCD1821522.1"
                     /translation="MFVDQVKISLKAGDGGNGITAYRREKYVPFGGPAGGDGGKGASV
                     VFEVDEGLRTLLDFRYQRHFKASKGENGQSSNMHGKNAEDLVLKVPPGTIIKNVETDE
                     VLADLVEDGQRAVVAKGGRGGRGNSRFATPRNPAPDFSEKGEPGEELDVSLELKLLAD
                     VGLVGFPSVGKSTLLSIVSKAKPKIGAYHFTTIKPNLGVVSTPDQRSFVMADLPGLIE
                     GASDGVGLGHQFLRHVERTKVIVHMIDMSGSEGREPIEDYKVINQELAAYEQRLEDRP
                     QIVVANKMDLPESQDNLNLFKEEIGEDVPVIPVSTITRDNIDQLLYAIADKLEEYKDV
                     DFTVEEEESVGINRVLYKHTPSQDKFTISRDDDGAYVVSGNAIERMFKMTDFNSDPAV
                     RRFARQMRSMGIDDALRERGCKNGDIVRILGGEFEFVE"
     gene            18498..18953
                     /locus_tag="NBI57_000547"
     CDS             18498..18953
                     /locus_tag="NBI57_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HCD1821523.1"
                     /translation="MDNKDYKKFYLIREDVLPESVVKTLKIKDALKSDPTLSIYDAVK
                     QFDLSRSAFYKYRETIFPVDDKMLDHREFTLILYVTDIVGMLARVLDVISKLELSVLT
                     IHQSIPMEEKATITLSLNAKSKETSVEDVIGALRNLDYVSKVELISMSM"
     gene            18967..19569
                     /gene="ruvA"
                     /locus_tag="NBI57_000548"
     CDS             18967..19569
                     /gene="ruvA"
                     /locus_tag="NBI57_000548"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HCD1821524.1"
                     /translation="MYAYVKGKLTHLYPTHVVVETAGVGYEIQTPNSYRFQKHLDHEV
                     LIRTSLIVREDAQLLYGFSSEEEKDMFLSLIKVTGIGPKSALAILATSTPNEVKRAIE
                     NENDTYLTKFPGIGKKTARQIVLDLKGKVKITEEDSDSLLQVDATSTVQDQFVQEAML
                     ALEALGYSKRELAKVEKTLNKNKYDSVDEAVKAGLQLVVS"
     gene            19601..20605
                     /gene="ruvB"
                     /locus_tag="NBI57_000549"
     CDS             19601..20605
                     /gene="ruvB"
                     /locus_tag="NBI57_000549"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001005768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HCD1821525.1"
                     /translation="MNERMVDQSMHSEETDFELSLRPTRLRQYIGQNSIKSNLEVFIK
                     AAKLRHEPLDHVLLFGPPGLGKTTLSNIIANEMEVNIRTVSGPSLERPGDLAAILSGL
                     QPGDVLFIDEIHRLSSVVEEVLYPAMEDFFLDIIIGKGDEARSIRIDLPPFTLVGATT
                     RAGSLTGPLRDRFGVHLRLEYYNESDLKEIIIRTAEVLGTGIDEESAIELAKRSRGTP
                     RVANRLLKRVRDFQQVNEDEQIYIETTKHALGLLQVDQHGLDYIDHKMMNCIIKQYNG
                     GPVGLDTIAVTIGEERITIEDVYEPFLIQKGFLERTPRGRKATPLAYEHFAKSNEERG
                     "
     gene            20607..21632
                     /gene="queA"
                     /locus_tag="NBI57_000550"
     CDS             20607..21632
                     /gene="queA"
                     /locus_tag="NBI57_000550"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HCD1821526.1"
                     /translation="MNIEEFDYDLPESLIAQTPLKDRDHSRLLVMDRETGEMKHLHFK
                     DIIEYFRPGDTLVLNDTRVMPARLFGLKEETGAKVEMLMLTQIEGNDWEVLLKPAKRI
                     KVGNKLNFGNGKIIAECIKEMDQGGRIMRLHYEGILQERLDELGEMPLPPYIKERLDD
                     PDRYQTVYAKESGSAAAPTAGLHFTDELLTEIKNKGVNIAFVTLHVGLGTFRPVSVDD
                     VNDHEMHSEYYQMTQETADLLNDTKSKGHRIISVGTTSTRTLETIRRDHDKFVETSGW
                     TNIFIYPGFDFKAIDGQITNFHLPKSTLVMLVSAFSTRENVLNAYKTAVNLEYRFFSF
                     GDAMLII"
     gene            21655..22794
                     /gene="tgt"
                     /locus_tag="NBI57_000551"
     CDS             21655..22794
                     /gene="tgt"
                     /locus_tag="NBI57_000551"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016425271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HCD1821527.1"
                     /translation="MPAVTYEHIKTCKQSGARLGIVHTPHGSFETPMFMPVGTKATVK
                     TMSPEELRQIEAKIILGNTYHLWLQPGNDIIKHAGGLHKFMNWDGPILTDSGGFQVFS
                     LSNLRKITEEGVEFRHHTNGSKLFLSPEKSMQIQNDLGSDIMMAFDECPPMPAEYDYV
                     KKSIERTTRWAKRCLDAHQRPEDQALFGIIQGGEYEDLREQSAKDLVELDFPGYAIGG
                     LSVGEPKPVMYKMVEHTEQFMPKDKPRYLMGVGSPDALIECSIRGMDMFDCVLPTRIA
                     RNGTCMTSQGRLVIKNAKFADDLRPLDENCDCYTCQNYSRAYIRHLIKAEETFGIRLT
                     TIHNLHFLLKLMEDIRQAIREDRLLDFKEEFFEQYGLNVENPKNF"
     gene            22813..23073
                     /gene="yajC"
                     /locus_tag="NBI57_000552"
     CDS             22813..23073
                     /gene="yajC"
                     /locus_tag="NBI57_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HCD1821528.1"
                     /translation="MQFSLLIYIVVIFAVMYFLMIRPQQKRAKQHRELINNIQSGQRI
                     TTIGGIKGTVKAVDETTVVITVNGHGTELTFEKPAIKQVDPS"
     gene            23348..25627
                     /gene="secDF"
                     /locus_tag="NBI57_000553"
     CDS             23348..25627
                     /gene="secDF"
                     /locus_tag="NBI57_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecDF"
                     /protein_id="HCD1821529.1"
                     /translation="MKKSSRIIAFLLLVVLLFAGMAATYKSVIKNVNLGLDLQGGFEV
                     LYQVDPLNKGDKIDKKALQSTAQTLENRVNVLGVSEPKIQVEEPNRIRVQLAGVTDQN
                     EARKILSSQANLTIRDAEDKVKLSGSDIKQGSAKQEFKQETNQPTVTFKVKDKNKFKK
                     VTEEISKKRDNVMVVWLDFKKGDSYKKEAQKKNPKFISAASVDQPINSDSVEISGGFK
                     GQEGVKKAKQIAELLNAGSLPVDLKEIYSNSVGAQFGQDALDKTVFASFIGVALIYLF
                     MLGFYRLPGLVAIIALTTYIYLTLVAFNFISGVLTLPGLAALVLGVGMAVDANIIMYE
                     RIKDELRIGRTIKQAFSKANKSSFLTIFDSNLTTVIAAAVLFFFGESSVKGFATMLLL
                     GILMIFVTAVFLSRFLLSLLVSSNIFKNQFWLFGVKKNKRHDINEGVDVHDLKTSFEK
                     WNFVKLAKPLIGVSILIVVVGLVILYIFKLNLGIDFSSGTRVDFQSKQAITQQKVEQV
                     VKDSGLKADQIQINGKDNKVATVQFKDDLTRAQDNKLSDNIKSKFGDTPQINTVSPII
                     GQELAKNAMLALIYASIGIIIYVSLRFEWRMGLSSVLALLHDVFIIVAIFSLFRIEVD
                     LTFIAAVLTIVGYSINDTIVTFDRVRENLQKVKVITTTEQIDDIVNRSIRQTMTRSIN
                     TVLTVIVVVVAILFFGAPTIFNFTLALFIGLISGVFSSIFIAVPLWGIMKKRQLKKSP
                     KHKLVVYKEKKSNDEKILV"
BASE COUNT         9007 a         3532 c         4934 g         8289 t
ORIGIN      
        1 atttcagatg tattctatac tttaagttgg ctgattattt caatctcttt aattcttaac
       61 cttatcaaag tattgaattt ctctgtgttt ttcttgaatt taatagggtt aacactttta
      121 gggatgaata catttcaacc gacacattat actaataagg ttcagaaaat tgctgttgta
      181 gacgagttac tgttagtcca tatcggtcta gctgtgttaa gttatgtctt ctttgcctta
      241 gcttttgtca atgcattact gtatatcatg caataccgta atttgaagga aaaacgtttt
      301 gatcaaaaat actttagaat aggtagcgtc gctacacttg aatcaattgt gttttactca
      361 acgttaagtg ggtggataat tctcatattt agtattattt taggtacaca gtggggtgta
      421 atttcagttg gtgaacgtat attcattgac ccgaaggtaa tactctcttc aattattaca
      481 gtattatatg gaagttatat attgcttaga atcaacaaat ggttgaattc aaggtattta
      541 atatactata acattatttt attttgctta aatatgatta atttattttt tgcaacacat
      601 tttgtgaatt gagaaaataa tctagagcaa tccgttccta ggaggaattg aacatgcgta
      661 aattagtcgt tggctccaga agaagtaaat tagctttaac acaaagccag caatttatta
      721 ataaattaaa agctgtcgag ccaaatctag aaattgaaat taaagaaatt gtcacgaaag
      781 gcgatcgtat agtagataaa caattgtcta aagtcggagg caaaggctta tttgttaaag
      841 aaatacaaca tgaacttttt gaaaaaaata tcgatatggc aatacactcg cttaaagacg
      901 taccaagtgt aattccggaa ggtttaacat taggttgtat ccctgataga gaattacctt
      961 ttgatgcgta tatttctaaa acacatacac cactatccca attgccagaa ggcagtatta
     1021 ttggtactag ttcattacgt cgtggtgcac aaatattatc taagtatcct aatttagaga
     1081 ttaaatggat tagaggtaat atagatacac gattagaaaa gttacaaact gaagattatg
     1141 atgcgattat tttagctgca gctggtttaa gaagaatggg ctggtcagat gatattgtaa
     1201 catcttatct tgatagagat acattgttac ctgcaatcgg acaaggtgct ttagggatag
     1261 aatgtcgtag tgacgatgaa gaactattaa cattattaag caaagtacat aatgatgagg
     1321 ttgcaaaatg tgtgactgct gaacgaacgt ttttagcaga aatggatggt agttgtcagg
     1381 tgccaatcgc aggatatgct acaatctcag atcaaaaaga aatcgaattt acaggtttaa
     1441 ttatgacccc agatggtaaa gaacgatttg aatatacaat gaacggaaca gatccggttg
     1501 agttaggcaa aacagtgagt aacaaattaa aagagcaagg tgcttatgaa attataaaac
     1561 gcttaaatga acaacattaa ataatggagt gacgtttaaa tatgaagcca gttgtagtta
     1621 tgacacaaac aaatgacatg caaagtgatt tagtatcaat tattcacaaa ccctttattg
     1681 atataaagcc actaaatttt gatattcatt tgttaaatca acgctatgac tggcttattt
     1741 tttcatctaa aaatgctgtg aaattctttt ataaatatct aaaaggaatt aatgttgata
     1801 acattgctgt gataggaagt aagacagcgc aatattgtga atcacttggc attcgagttg
     1861 attttatgcc aaacgacttt tctcaagaag gatttttaaa atcatttaat caaactaacc
     1921 aaaaaatact tttgccttcg agtgaattgg cgagaccatt gttattagca gcgttatcta
     1981 aagataatga agttgttaaa atagatttat atacttcagt gcctaacaaa caaaatatac
     2041 aagatgttaa agaaatgata gaacatcaac aaatcgatgc attaacattt tcaagttcgt
     2101 cggcagtacg ttattatttt aatgaaggat ttgtaccaaa attcaagtcg tattttgcta
     2161 ttggagaaca aacagcacgg accattaaaa catatcaaca accagtaaca attgcagaaa
     2221 ttcaaacact cgaatcacta attgaaaaga ttttagaaag taggggctaa aaatgaaatt
     2281 tgatagacat agaagattga gatcatcagc gacaatgaga gatatggtta gagagaatca
     2341 tgtaagaaaa gaagatttaa tatatccaat ttttgtagtt gaaaaagacg atgtgaaaaa
     2401 agaaattaag tcattgccag gtgtatacca aatcagtttg aatttacttg aaagtgaatt
     2461 aaaagaagct tatgacttag gcatacgtgc cattatgttt ttcggtgttc caaactcaaa
     2521 agatgatata ggtactggtg catacattca cgatggtgtt attcaacagg caacacgtat
     2581 tgctaaaaaa atgtatgatg acttattaat tgttgcagac acttgtttat gtgaatatac
     2641 tgatcatggt cattgtggcg tgattgatga ccatacacat gacgttgaca atgataaatc
     2701 attgccacta cttgttaaaa cagcaatttc tcaagtggaa gctggtgctg atattattgc
     2761 gccaagtaat atgatggatg gttttgttgc tgaaattcgt cgtggattag atgaagccgg
     2821 ctattacaat attcctataa tgagttatgg tgtcaagtat gcatcaagtt tctttggacc
     2881 ttttagagat gcagcagatt cagcgccatc atttggggat agaaaaacgt atcagatgga
     2941 ccctgctaac cgtttggaag cacttcgtga attagaaagt gatcttaaag aagggtgcga
     3001 catgatgatt gttaaacctg ctctaagtta tttagacata gttcgagatg ttaaaaatca
     3061 tacgaatgtt ccagttgttg catataatgt gagtggagaa tatagtatga ctaaagcagc
     3121 ggcacaaaat ggttggatag atgaagaacg tgtcgttatg gaacaaatgg tttcaatgaa
     3181 acgtgcaggt gctgatatga ttattacgta ttttgcaaag gacatttgtc gctatttaga
     3241 taaataaggt tttatatgta tgattttcca taaactatag gaggaattta ctttatgaga
     3301 tatacgaaat cagaagaagc aatgaaggtt gctgaaactt taatgcctgg tggtgtaaat
     3361 agtccagtac gcgcatttaa atcagtagat acaccagcaa tttttatgga tcacggtaaa
     3421 ggctcaaaaa tttatgatat cgatggtaac gagtatatcg actatgtact aagttggggg
     3481 ccgcttattt taggacatag agaccctcaa gttattagtc atttacatga agcaattgat
     3541 aaaggtacaa gttttggtgc atcaacatta cttgaaaata aattggcgca gctcgttatt
     3601 gaccgagtac cttcaataga aaaagtgcgt atggtgtcat ctggtacaga agctacattg
     3661 gatactttaa gattagcacg tggttatact ggcagaaata aaattgtgaa atttgaaggt
     3721 tgctatcatg gtcatagtga ttcgttatta atcaaagctg gttctggggt ggcaacatta
     3781 ggattgccgg attctcctgg tgtgcctgaa ggtattgcta aaaatacaat tacagttcca
     3841 tacaatgatt tagatgcact taaaatcgct ttcgaaaaat ttggagacga tattgctggt
     3901 gtaatcgtag aacctgttgc tggtaatatg ggtgtcgtac caccgattga aggtttttta
     3961 cagggattaa gagatattac gactgaatac ggcgcattgc taattttcga tgaagtaatg
     4021 actggtttca gagtcggtta tcattgtgca caaggttact ttggtgtgac accagattta
     4081 acttgcttag gaaaagttat cggtggagga ctacctgtag gtgcttttgg tggtaaaaaa
     4141 gaaatcatgg atcatatagc accattagga aatatttatc aagcgggtac gttatcagga
     4201 aatcctcttg caatgacaag tggttatgaa acgttaagcc aattaacgcc agagacatat
     4261 gagtatttta atatgttagg cgatatactt gaagacggtt taaagcgtgt atttgctaaa
     4321 cacaatgtac caataactgt aaatagagca ggttcaatga ttggttattt cttaaatgaa
     4381 ggacctgtaa ctaattttga acaagcgaat aaaagtgatt tgaaattatt tgcagaaatg
     4441 tatcgagaaa tggcaaaaga aggtgtgttt ttaccaccat ctcaatttga aggtacattc
     4501 ttatctacgg cacacacgaa agaagatatt gaaaaaacga ttcaagcatt tgatacggct
     4561 ttaagtcgta ttgtaaaata aatatacgga caaattgaga gcctgaactt tgttcaggct
     4621 ctttttaaat gtatatcagg catgggcggc gacttgatag tgaaagtcca tatatgcttt
     4681 gtagtcaaaa ctgctagcgg atattgttat cttaacaaac gtgaagctca agcagcaatt
     4741 tagtcatttt attttttatt gaaagaagtg gaaaacatga caatgatata tagaaataat
     4801 ttcattgtgt tcgttttatc attttttatt agtattatat tgtattcatc gcacgtatta
     4861 ctcccattta tgtttggtcc tattatcgca tcaatcattt gtgtgaaagt tttcaaactt
     4921 gatattaaat ggccattctt acttagtgaa ttagggattg tactattagg ggtgcaaatc
     4981 ggatcaacgt ttacgaaaaa tgtcgttatg gatattaaaa acaattggct ttcgattatt
     5041 gttgtatcta tttcgatatt attaattgca atagtaatgg catttatttt taaaaaaatt
     5101 gcacgtatta atacagaaac agctatttta agtgttatac caggagcact aacacaaatg
     5161 ctggtcatgg ctgaacaaga caaacgtgct aatttgttag ttgttagctt aacgcaaaca
     5221 tcacgaatta tatttgttgt tgttttagta ccgttcattt catatttttt tcatgatggt
     5281 aacatgcatg cgaatggaaa gttaacaaaa gtcttgcctt tatcacaagt attaaacata
     5341 gggcaaatag ttattttagc gatagctatc tttatagttt atctaattat gtctaaaata
     5401 aagtttccaa catttcaatt attagcacca ctcattgtat taattgtttg gaatttttct
     5461 acaggtttaa catttacact agatcattgg ttgttgaaca tggcacaact aatatatatg
     5521 attagaattg gagttcaaat tgcgcattta ttgtcagatt taaaaggtag actagcaatc
     5581 gcaattacaa ttcaaaatat tatgttgata attggtgcgc taatcatggt ttatatcata
     5641 catttctttg ataataatcc tattaatgaa ttgtttcttg gtgcagcacc aggaggggtt
     5701 aatcagatag ttttagttgc attagctact ggtgccgatg ttgctatgat tacgagttat
     5761 catattttcc gaatcttttt catcctattt attattgcac caggaattaa ttacttttta
     5821 aaatatcgtt cgaataagcg tgaagattag aaactatcat ccatttaagt tgaattatgc
     5881 ttaggataga attcattatt aaggtttaag caaaggttgt acatctattt ttagattaag
     5941 atatctaata ttagcagtgc attaacttgg aactttaact tatgatttga ataaattttc
     6001 acatcaaaaa acgagacagt ctattagtta aaaatagatg atgtctcgtt tactttatga
     6061 tgctatagca aattaattgt gtttaggctt tgatggacaa tcttttaaat gtgcatcata
     6121 taaaccagct gcttctaaaa acgaaaatac tgttactgga cctaaaaatt taaacccgta
     6181 ttgttttaaa tctttagata gttgtgttgc agtatcatca actgtgatac gatcagaagc
     6241 atgttcatac tgcaaatctt taggcttacc atttacataa gaccataaaa atttactaaa
     6301 actaccatat gcttgttcaa tttttaaata cccttgagct tgattaacaa ttgcttctaa
     6361 ttttttacga tgatgaacga tatttggaaa agtcattaag cggtcgatat cttgagcggt
     6421 catttgtgct accttttctg gttcgaaatc ataaaatgct tcttcatagg cttctttctt
     6481 ttttaaaata gttaaccaag atagcccagc atgttgtgat tctaatgcta aaagtttaaa
     6541 caatgccttg ctatcataga gcggttgtcc ccatacatga tcatgatagt ttaagtagac
     6601 tggatcttta gtaccaaatg cgcattcatt cattttattc ccacctatac attgacttat
     6661 tttgtcaatc attatactat aagttaagag tttaatatag gatttgggaa gagtaagttc
     6721 aaattatttt aaagagagca tatgatttgg tgtgagtatg taaatgattt ttactgaagg
     6781 tagcccacag agtatcttag ctgaacgtga ttaatagtag gctaagacgt gtttgagcgt
     6841 taatcataat ctagagtgct tgaagagatg aagcatttca aatttcacga tgatagtatt
     6901 ggtatgatta ttccaattat gttaaaagag tgaatttgat attaaacatc cttcttgaat
     6961 ataggtggta ccacggaata tccgtcctat ttgtatatag gatggatatt tttatttttt
     7021 taggaggtat agcaaatgga aatgaaacca aaatatgatc ctcgtgaagt tgaagcggga
     7081 cgttatgaag aatgggtaaa gaatggttat tttaaaccgt cagaagataa atcaaaagaa
     7141 acatatacaa ttgttatccc gccaccaaat gtaactggta aattacattt aggacatgca
     7201 tgggatacga ctttacaaga tatcattaca cgtatgaaac gtatgcaagg atacgatacg
     7261 ttatacttac caggtatgga tcatgctggt attgcgacac aggcaaaggt agaagctaaa
     7321 ttaaatgaac aaggaataac tagatatgat cttggtcgtg aaaagttttt agaacaggca
     7381 tgggattgga aagaagagta tgcgtcattt attcgtgcgc aatgggctaa attaggtcta
     7441 ggtttagatt atagtagaga acgttttact ttagatgaag gtttaagtaa agcagttaaa
     7501 aaagtttttg ttgatttata caataaagga attatttatc gtggcgaacg tattataaat
     7561 tgggatccta aagcacgtac agctttatct gatattgaag taatacatga agatgttcaa
     7621 ggtgcgtttt atcattttaa atatccttac gctgatggtg aaggttttat tgaaattgca
     7681 acaacaagac cagaaacgat gttaggtgat acagcgattg ttgttaaccc taatgacgaa
     7741 cgatacaaag atgtaatcgg taaaactgtt atattaccaa tcgtaggacg cgaactgcct
     7801 attttagcag atgagtatgt tgatatagac ttcggttctg gtgctatgaa agtgacacca
     7861 gcacatgacc ctaatgattt tgaaattggt caaagacatc aattagaaaa tattatcgtt
     7921 atggatgaaa atggtaaaat gaacgacaaa gcgggtaaat atgaaggtat ggaccgtttt
     7981 gattgtcgta aacagctagt taaagattta aaagaacaag atttagttat caagattgaa
     8041 gatcatgttc attctgtagg tcattcagaa cgatctggcg ctgttgttga accatattta
     8101 tcaacacaat ggtttgtgcg catggaagac ttagcgaaac gttcattaga taaccaaaaa
     8161 acagatgatc gtattgattt ttatccgcaa cgtttcgaac atacatttaa ccaatggatg
     8221 gaaaatatta gagattggac gatttcaaga caattatggt ggggtcatca aattccggct
     8281 tggtatcata aagaaacagg cgaaatatat gttggagaag aagcgccaac tgatattgaa
     8341 aattggcaac aagatgaaga tgtattagat acgtggttct caagtgcttt atggcctttc
     8401 tctacgttag gttggcctga tttagaaagt gaagacttta aacgatacta cccaacaaat
     8461 gccttagtta caggttacga tattatcttt ttctgggtcg cacgcatgat attccaaggc
     8521 ttagaattta cagatcgtcg tccatttaat gatgtattat tacacggttt agttcgtgct
     8581 gaagacgggc gtaagatgag taaatcatta ggtaatggtg tggatccaat ggatgttatt
     8641 gacgaatacg gtgctgatag cttgcgttac ttcttagcaa caggttcatc tccaggacat
     8701 gatttaagat actcaactga aaaagttgag tcagtgtgga actttatcaa taaaatctgg
     8761 aatggggcac gtttcagttt aatgaatatc ggtgaagact ttaaagttga agatatcgat
     8821 ttaagtggta acttatcatt agcagataaa tggattctaa cacgtttaaa tgaaacgatt
     8881 gcaacagtta ctgatttaag tgacaaatat gaattcggcg aagttggacg tgcattatat
     8941 aatttcattt gggatgattt ctgtgattgg tacattgaaa tgagtaaaat tccaatgaat
     9001 agtaatgatg aagaacaaaa acaagttaca cgttcagtat tgagttatac tttagacaat
     9061 attatgagaa tgctacatcc attcatgcca tttgtaacag agaaaatatg gcaaagttta
     9121 ccacatgaag gtgacacaat tgttaaagct tcatggccag aagtgcgtga atcattgatt
     9181 tttgaagaaa gtaaacaaac aatgcaacaa cttgttgaaa tcattaaatc tgtaagacaa
     9241 tcacgtgtag aagtaaatac gccattgtct aaagaaatac ctattttaat tcaagctaaa
     9301 gataaagaaa ttgaaacaac tttatcacaa aacaaagatt atttaatcaa attctgtaat
     9361 cctagtacct taaatattag cactgacgtg gaaattcctg agaaagcaat gacatcagtt
     9421 gtaattgcag gtaaagtggt attaccatta gaagggctaa ttgatatgga taaggaaatc
     9481 agccgtttgg aaaaagaatt agctaaactt caaagcgaat tagatagagt agataaaaag
     9541 ctctctaatg aaaactttgt aagtaaagca cctgaaaagg ttataaatga agaaaaacgt
     9601 aaaaaacaag attatcaaga aaaatatgat ggtgtgaagg caagaattga acaattaaaa
     9661 gcataggagt tagtaacaat gaattaccta gagagcttgt attggataca cgaaagaact
     9721 aaatttggca tcaaaccagg tgttaaacgt atggaatgga tgctagcaca atttaataat
     9781 cctcaaaata acattaaggg tattcatgta ggtggcacaa atggtaaagg ctctacagtt
     9841 gcttacctta gaacagcttt agttgaaaat ggttatgaag taggtacatt tacgtcgccg
     9901 tttattgaaa catttaatga acgaattagt ctaaatggtg tgccaatatc aaatgacgct
     9961 attgtagaat tagtatcacg tattaaacca gtaagtgaaa tgatggaacg tgaaacagat
    10021 ttaggtgttg caactgaatt cgaaataatc acagcgatga tgtttttata ttttggtgaa
    10081 atacatcctg ttgattttgt cattgttgag gctggattgg gtataaagaa cgattcgaca
    10141 aatgtcttta caccggtttt atcaatctta actagtatcg gtctagacca tacagatatt
    10201 ttaggtggta cttatctaga tattgctagg gataaaggcg cgattataaa gcctaacgtt
    10261 ccagtgatat atgctgttaa aaatgaagat gcattaaaat atgttcgtga acgcgcaatt
    10321 gaacaacatg caaagccaat tgaattagat agagaaattg ttgttgtatc gcaaaatgat
    10381 gaatttactt accgttataa agattatgaa ttagaaacaa tcattttaag catgttaggt
    10441 gaacatcaga aacaaaatgc tgcattagcc ataacagctc ttattgaatt aaatgaacaa
    10501 ggattaattg aattagattt caataagatg atagacggta ttgaatcagt tcgttggact
    10561 ggacgtattg agcaggtgca tgacaaacct ttaatcattt tggatggcgc acataattca
    10621 gagagtatag atgctctaat tgatacaatt aaacagtacc atgataaaga aaaagtagat
    10681 attttgttct cagcaataaa cggaaaaccg attaacgaga tggtcaaaca tttaagttta
    10741 attgcgcata cgttttatgc aactgaattt gattttccga aagcgttacg caaagaagaa
    10801 attgtaggta gtattgaaaa tgatgaaata caattagtag atgactacgt tgaatttata
    10861 aaaaattatc aaggtgatac attagtaatt accggtagtc tgtatttcat aagtgaagtt
    10921 aaatcaaaat tagactttga agcattataa aactaaaact aaaatagcca tgaagaatct
    10981 atttaatcaa taggtagagc ttcatggtta ttttttatgc tctaaatcgt atgtgtttat
    11041 aaggttcaat ttcttgccat tattaatttt gccgagcagt aatgggttgt taaagtatcc
    11101 atcaattatg cttagcaata ttgaaaaatt taaatgaaaa agcggttcaa ttcttgaaaa
    11161 atagtgtata tttacatttt ggtaataact aactataatt tacataagga ggttgtcatt
    11221 tggtagtatt gttatcttat agctgcagtt gtatttttag ttttctatat caatttatat
    11281 ctatagaaga aacgtcattt gattatttac atagaagatc gaaatgtgat tattgtaatt
    11341 cgtcactcaa atggtatgaa ttaatgccga ttattagttt tttattatta aaagggcgat
    11401 gtcgaaactg tcgaaagcgt atttccctaa cacatttctt aggggaaacc tttgctttaa
    11461 tacctatcgt ctttattaag tatgatttca catacgtaaa tgctacgcta tttataacta
    11521 catatgtttt tctgcttatt tttactatga ccgatatcac ttctttaatg ttagattgtc
    11581 gcttaattat aatttattgt atcgtttctc tctcgttaag tatgatttat ccagtagctt
    11641 ttatcattat tagtatgacc acgcatatat tctacttttt atttcgggca tatattggtt
    11701 atggtgacgt tttactaata tctgcacttt ctttgttttt ccctctccaa ttcactattt
    11761 atgtcatttt atttacattt gtcattgctg gtttagttgc tttaattacc atgatattta
    11821 agccgattaa actattaccc cttgttccat ttatatttat ttcctttttt atcaattcac
    11881 ttttttataa tgatatccat caatttttag gaggcgtata tttttgaaaa ttaaagaaat
    11941 ggtaacttca gaaatgccaa gagaacgttt gttaagccat ggtgcaaaaa gtctttcgaa
    12001 tacagaatta ttagctatat taattaacac cggaagaaaa ggattctcga gcatagacat
    12061 tagtaatgaa ctgcttaaat ctgcttcgaa tctgaatgaa ttgaaaaaat cttcaattaa
    12121 cgatttgata aaagttaaag ggattggatt acaaaaagca attactttaa aagcagcatt
    12181 tgagttaggg gaaaggatgg ggagaagagc tgaaaataat cgtataaaaa taacgcaacc
    12241 aagtgatgtt gctgattata tgattccaac aatgaaagat ttaacacaag aacattttgt
    12301 cattttattg ttaaattcaa aaaatgtagt gattaaagaa acctgtgttt ttaaaggtac
    12361 attaaatagt tcgattgtac atccacgtga aatttttagt attgcggtga gagaaaatgc
    12421 caatgcaatc atcgcagttc ataatcatcc atccggtgat gtaacgccct cacaagaaga
    12481 tatcataaca acaatgaggt tgaaggagtg tggtttgatt ttagggatag atttattgga
    12541 gcatattata atcggtgata atagatttac cagtcttgta gaagcgggtt actttgatga
    12601 aaatgattga agttgataga attaatgacg tatcttgtgt ataataccta cgaagtactt
    12661 tcattggagg aaaaatagtg actctattta ttattatcgg ggttctcgtg ccaatggttt
    12721 ataccatgca gttaaatatt aaaaatgaac ctgtaacaaa gcgcaatcta ttaataacat
    12781 tagctttatc tacgttaggt attttagtaa cggcgttagc aggtgtgatc gttacgaaac
    12841 aagcttttcc tttattaagt gtagcaatcg gctcaatttt tactggaatc gtttggggcc
    12901 ttttactaag tggtagctac gcgctgatac gatttttatc taacgcattt gggcgtaagt
    12961 aatagtaatc tgttcatcaa gtagtatccg tgcttgaaac caaattaaaa ctcctaatgt
    13021 ggaactagtt aaaaaattct aaactagtac attaggagtt ttgttatgca gaataaattt
    13081 aattgttaaa ttgaaagtgc ggtaaaaacc cactatttat ttgaaaaaaa tcgagcaacc
    13141 aaattaaacc ttgatatcct aagtaaatac ataataaaac aagtccgaca taaattaaaa
    13201 atcgcaaaat agaaagtcca actctaaaaa ggaggatgac taatagtgct attaaaataa
    13261 ttgttagtat actcaatgct acaaacctcc tcatacgctt ttaaatcata acaaaattat
    13321 aagctatttt aaatgaacgt gcatcagtcc taagatatat catgattaag gctgaagact
    13381 gaggaaaata tacataataa aatatataat tagatgaaga cattggaggc gaactatgag
    13441 attgatattt agtatgatta aaaacattat agcagtaata gcaattgtga taattgttta
    13501 tatcgccttg aaatacgcac cttttttaaa agaacaagat tggaatcctg taaatcacga
    13561 taatgatcaa atgaatcaag ttacgcaacc cactaatgat gcccaacatg tttatgtaag
    13621 tggtgagaaa tattcattaa aagaaaatga tttaataaag aatgtaccat taagccaaat
    13681 taaaaatgta tttaaaatga tagataaaca agagtttatg gctgtgtctg gaatgaatcg
    13741 catggcttat aatgatcaat atattatagg tcaaagagga gacgaattta ttctttataa
    13801 atttggagat gagtcaatgc gtgtttacaa tactgaattt gaaatgcaac aagacttaaa
    13861 tgaattaggg caaaatttac aactaaaacc cgaaaatgct tatcaatagg aaattattgc
    13921 ataagaaata tattttagtt atcctgtcac tagaataaat ttaaaaggtt ggtgtagttc
    13981 aaatggcaga acaatcaaaa cagaaacaag ctaatgaaca acaaaaggcc cagaacttat
    14041 tcgcacgttg gagacaactt cagaattcga acagtgaatc ctcgaacgat acaaacaaat
    14101 aattttaaaa tttaaatcga ttatttaaac tactagtgac tggaatgttt gctagtagtt
    14161 ttttgttttt aaaatgccac aaatttttta cggagttgtt cattttcaaa gtaattcgta
    14221 gttagtgctt tatttcataa tgggagtaat ataaaataga aaaggatata caatattttg
    14281 acataataga ggtgttctgg gtgcttaagt tttttaaaaa taacaaatta attgttgttt
    14341 tatgtgcaat tatcgttttt attgcattaa ttgggctgtc catacgttca caatctcaat
    14401 cacctcctga acaatatata ggtgattctg tgtcttttgg acaacgagtt gtgagttatc
    14461 cagttaattt tgttgctggt acgattgggg acttttttaa aaaaggagat tctaaagaat
    14521 ctaaaaataa gattagccag ttagaatcta agaaccaaca attagaagcg gaaaatgaaa
    14581 aattaaaaaa agagcttgat ttaaaagata tttcaaaatt tgatcctatt tctactacgg
    14641 ttttagcaag aaatccggat cagtggatga atacaattgt aattgataag ggatctaaat
    14701 caggtataac ttcaaatatg gctgtgatga catcacaagg ttttgttgga agagttacta
    14761 aagttaataa attttcttca caagttgatt taatctcaac taatacacgt gcgggtaaat
    14821 tatctgtaaa tatacaacac ggttctaaaa atatatttgg tttaattgat cgttatgatg
    14881 aaaagaactc agaacttgta attagtgaca ttaataatag agataatatc tcaaaaggtg
    14941 ataaagtcgt tacaagtgga ttagctgatc aactaccaag taatttatat ataggagaag
    15001 tgactaaggt tcaaaatgat caatacggct tagctaaaga agttagggtt aagactggtg
    15061 cagacttaac agatttgagt catgtttatg ttgcaaaaag agatcctaaa acaattcctg
    15121 atgatgaaag cagggataaa taatgcgtac actgtattat tttttgatag gtattttact
    15181 attttatata gatactgcaa ttgggcttct tattccaatg catataggta agtttgaact
    15241 tgtatttgta ccacacctta catttatgta tattttaatg atggtagtgt atcgtggctt
    15301 tggcgtatca ttattgctca gcatattttt aggcgtaatg actgatgtat actttggtag
    15361 tatttatgga gtgtacttat ttggctatat attgttttta gcacttatag atcgattctt
    15421 taaaattttt tacaaagatc attcgatgtt attcattatt atattagcca gcaccttatt
    15481 attagaagtc tatgtggcat taatatacgg tatgttagga ttcattcaat ttgatattat
    15541 tcattttgta gtctttagat tattgccaac attaattatg aattttgtac tgttgattat
    15601 gctttatccg ttgattataa agttccttaa aaaaacaaac aatgacattg acatgaaacg
    15661 tcgtcaatgg taagatgcaa tagttgagta gttataagta gctacataca accgctcaaa
    15721 tataggttca agaacatttt taatgaagaa agcgattgtc aatcaaggcg ttgaaggaag
    15781 aaacgagaag agtttatgtt tataaatgac tcgagttgcg accgaaacaa cgtagagtgc
    15841 aaacgcttga ctcaataacg aaatgtcacc tatgtatggc gtgacttatt atacaggagg
    15901 tgcaaagtat gtttgctatt attgaaacag gtggaaaaca aatcaaagta gaagaaggtc
    15961 aagaaatctt cgttgaaaaa ttagacgtaa acgaaggaga tacttttaca tttgataaag
    16021 tattatttgt aggtggagat tcagttaaag ttggagcgcc aacagttgaa ggtgcaacag
    16081 ttactgctac tgttaataaa caaggtcgcg gtaaaaaaat cactgtattc acatacaaac
    16141 gtcgtaaaaa ttcaaaacgt aaaaaaggcc atcgtcaacc atacactaaa ttaacaatcg
    16201 ataaaatcaa cgcgtaatta ttatgattac tgttgatatt acagttaatg atgaaggcaa
    16261 agtaacagac gttattatgg atggccatgc tgaccatggt gaatatggtc atgatatcgt
    16321 ttgtgctgga gcttcagctg tattgtttgg tagtgttaat gcgattatag gattgacatc
    16381 tgagagacca gatatcaatt atgacgacaa tggtggtcat tttcatataa gaagcgttga
    16441 tacaaataac gatgaagcgc aactaattct tcaaacaatg cttgtgtctt tacaaactat
    16501 tgaagaagaa tataatgaga atattagatt aaattataag tgaggtgcat tccgatgtta
    16561 aaattaaact tacaattctt cgcatctaaa aaaggggtaa gttctacaaa aaacggacgt
    16621 gactctgaat caaaacgctt aggtgctaaa cgtgctgacg gtcaattcgt aacaggtggt
    16681 tcaattttat atcgccaacg tggtactaaa atttaccctg gtgaaaatgt aggtcgtggt
    16741 ggcgatgata cattattcgc taaaatcgac ggcgttgtta aattcgaacg taaaggtcgc
    16801 gacaaaaaac aagtttctgt atatgcagta gctgaataat tttgtctagt taacaccaga
    16861 agtgaatctt ctggtgtttt ttacttttta taaaaggttt ttcattatta ttttaattat
    16921 gttattaaaa aatgggaatt ttagacgtta tactaaatgt acaactgcat aagagcccct
    16981 aatcgctaaa ctataagggg acaaaagaat acagttgcag tataaaaagc aaaaaaagta
    17041 tcatatttgg tattcggaaa tatcagcaat ttgagtaaag taaatttaaa agcaactgca
    17101 taagagcccc taattattta caatatataa ggggctctaa tatgctataa ttattgggaa
    17161 aatgaaaatt atatgtaaaa gaggtgagat atatgtttgt cgatcaagtc aaaatatctc
    17221 ttaaagccgg tgatggtggt aatggtatta ccgcatatag aagagaaaaa tatgtaccat
    17281 ttggtggacc agctggcggt gacggtggta aaggtgcttc agtcgtattt gaagtggatg
    17341 aaggtttaag aacgttatta gattttagat atcaacgtca ttttaaagca agcaaaggtg
    17401 aaaatggcca aagtagtaat atgcatggta aaaatgcgga agatttagta ttaaaagttc
    17461 cacctggtac aattattaaa aatgttgaaa cagacgaagt gttagcagat cttgttgaag
    17521 atggtcaaag agctgtagta gcgaagggcg gtcgaggtgg ccgaggtaat tcacgttttg
    17581 caacacctag aaaccctgca cctgacttca gtgaaaaagg tgaaccaggt gaggaattag
    17641 atgtatcttt agaattgaaa ttattagctg atgtaggatt agtaggtttc cctagtgtgg
    17701 gtaaatcgac tttattatct atcgtttcaa aagctaagcc taaaattggg gcatatcatt
    17761 ttacaacgat taaaccaaat ctaggtgttg tttcaacgcc tgatcaacgt agttttgtta
    17821 tggcagattt accaggttta attgaaggtg catctgatgg cgttggatta ggacatcaat
    17881 ttttaagaca tgtagagaga acaaaagtta ttgttcacat gattgatatg agcggttctg
    17941 aaggtagaga acctattgaa gattataaag tcattaatca agaattagct gcgtacgagc
    18001 aacgtttaga agatagacct caaatcgtag tagctaacaa gatggattta cctgaatcac
    18061 aagataattt aaacttgttt aaagaagaaa ttggcgaaga tgtgccagtt attccagttt
    18121 caacaataac gcgtgataat attgatcaat tattatatgc aatagcagat aaattagaag
    18181 aatataaaga tgttgacttc acagttgaag aagaggagtc agttggcatt aaccgagtat
    18241 tatataaaca tacaccgtca caagataaat ttacaatttc aagagatgat gatggtgctt
    18301 atgtggtaag tggtaatgct attgaaagaa tgtttaaaat gactgacttt aacagtgatc
    18361 cagcagtacg tcgatttgct cgtcaaatgc gttcgatggg tattgatgat gcacttagag
    18421 aacgtggttg taaaaatggt gatatcgtta gaattcttgg cggagaattt gaattcgttg
    18481 aataggagcg taacatgatg gacaataaag attataaaaa gttttattta attagagaag
    18541 atgtcttgcc tgaatccgtg gttaaaacat tgaagattaa agatgcctta aaaagtgatc
    18601 cgacattgtc catttatgat gccgttaaac agtttgatct atctagaagt gctttttata
    18661 aatatagaga aacgatattt ccagtagacg ataaaatgct tgaccatcga gaatttacat
    18721 taattttata tgtaactgat attgtgggta tgttggcacg tgtactagat gttatatcaa
    18781 agttagaact atctgtatta acgattcatc aaagtattcc aatggaagaa aaagcaacaa
    18841 taacattatc actgaatgct aaatctaaag aaacttcagt agaagatgtt attggtgctt
    18901 tgagaaattt agattatgta tcaaaagtag aattaattag tatgagtatg taaggatgtg
    18961 cctataatgt acgcgtatgt caaaggtaag ttaacacatt tatatcctac acacgtagtt
    19021 gttgaaactg ctggtgttgg ttatgaaatt caaacaccaa attcttatcg ttttcaaaag
    19081 catctagatc atgaagtttt aattcgtaca tctttaattg ttcgtgaaga tgcacaatta
    19141 ttgtatggat ttagtagtga agaagagaaa gatatgttct tgagtttaat taaagttact
    19201 ggtattggtc cgaaatcagc tttagctatt ttagcgacaa gtacgcctaa tgaagtaaaa
    19261 cgtgccattg aaaatgaaaa tgatacgtat ttaactaaat tcccaggaat tggtaagaaa
    19321 acggcaagac agattgtctt agatttaaaa ggtaaagtga aaatcactga agaagatagc
    19381 gattcattat tacaagtaga cgctacttcg acggtgcaag atcaattcgt gcaagaagca
    19441 atgttagcgt tagaagcatt aggttattct aaacgagagc ttgcaaaagt tgagaaaacg
    19501 ttaaataaaa ataaatatga ctcagttgat gaagctgtta aggcaggtct tcaattagtt
    19561 gtatcttaat tttaaataga ttaatagggg aagtgttgtc atgaatgagc gtatggttga
    19621 tcaatcaatg catagtgaag aaactgattt cgaattgtcg cttagaccta cgagattacg
    19681 acaatatatt ggtcaaaatt caataaaaag taatttagaa gtatttatta aagcggctaa
    19741 acttcgtcat gaaccattag atcatgtatt gctttttggc ccccctggat taggtaagac
    19801 aacattatct aatatcattg ccaatgaaat ggaagttaat atacgtacag tatcagggcc
    19861 ttcattagaa agacctggtg atttggctgc aattttatca ggacttcaac ctggagatgt
    19921 attgtttatt gatgaaatac acagactgag tagtgttgtt gaagaagtgt tataccctgc
    19981 aatggaagat ttctttttag atattatcat tggtaaaggc gatgaggcta gaagtatccg
    20041 tatcgactta cctccattca ctttggtagg tgcaacaacg cgagctggca gcttaacagg
    20101 tccactaagg gatcgatttg gtgtgcactt aagattagaa tattataatg aatcagattt
    20161 aaaagaaatc attattagaa cagctgaggt tttaggcaca ggtattgatg aagaaagtgc
    20221 cattgaactt gctaaacgtt ctagagggac tccaagagta gcaaatcgac tattgaagcg
    20281 ggtaagagac ttccagcaag tgaatgaaga tgaacaaata tacattgaaa caacgaagca
    20341 cgcattaggt ttacttcaag ttgatcaaca cggactagat tacattgatc ataaaatgat
    20401 gaactgtatt attaagcagt ataatggtgg acctgttggt ttagatacga ttgccgtaac
    20461 aattggtgaa gaacgtatta caattgagga cgtttatgag ccatttctta ttcagaaagg
    20521 ctttttagaa cgtacgccac gtggcagaaa agcaacacca ttagcttatg aacattttgc
    20581 aaagtcgaat gaggagagag gataacgtga atattgaaga atttgactat gacttaccag
    20641 aatcattaat tgctcaaacg cctttaaaag atcgtgatca tagtcgttta ttagtcatgg
    20701 atagagaaac tggtgaaatg aaacatttac atttcaaaga tatcattgag tattttagac
    20761 ctggtgatac attagtgctt aacgatacgc gagtaatgcc agctagactt tttggtttaa
    20821 aagaagaaac tggtgcaaaa gttgaaatgt taatgttaac tcaaattgaa ggtaatgatt
    20881 gggaagtctt actgaaacca gctaagcgta ttaaagttgg taataaattg aattttggta
    20941 atggcaaaat tatagctgag tgcataaaag aaatggatca aggtggacgc atcatgcgtt
    21001 tacattatga aggtatttta caagaaagat tagatgaatt aggggaaatg ccactgccac
    21061 catacatcaa agaacgttta gatgatccag atcgttatca aacagtttac gctaaagaaa
    21121 gtggttcagc ggcagcacca acagcaggat tacattttac tgatgagtta ttaactgaaa
    21181 ttaaaaataa aggcgttaat atcgcatttg ttacattaca tgttgggtta ggtacgttta
    21241 gaccggtgag cgtcgatgat gtgaatgacc acgaaatgca tagtgaatat tatcaaatga
    21301 cacaagaaac agctgattta ttaaatgata ctaagtccaa aggacatcgc attatatcag
    21361 ttggtacaac ttcaacacgt acacttgaaa caattcgacg cgatcatgat aaatttgttg
    21421 aaacgagtgg ctggactaat atatttattt atccaggatt tgattttaaa gcaattgatg
    21481 gccagattac taattttcat ttaccaaaat caacattagt tatgctagta tcagcgttta
    21541 gtactcgtga aaatgttctg aatgcttata aaacagcagt aaatttagaa tatagattct
    21601 ttagttttgg cgatgcaatg ttaattatat aaaaagaatg tgaggatttt gaatatgcct
    21661 gcagtaacat acgaacacat taaaacttgt aaacaatcag gtgcgcgttt aggtatcgtg
    21721 cacacaccac acggttcatt tgaaacacct atgtttatgc cagttggtac taaagcaacc
    21781 gttaaaacaa tgagtccaga agagttaaga caaattgaag caaaaatcat tttgggcaac
    21841 acatatcatt tgtggttaca acccggaaat gatattatca aacacgctgg gggattacat
    21901 aaattcatga attgggatgg tccgattctt acagattcag gcggtttcca agtgtttagt
    21961 ttaagtaatt tacgtaaaat tacagaagaa ggcgtggaat ttagacatca tactaatggg
    22021 tctaaattat ttttgagtcc tgagaaatca atgcaaattc aaaatgattt aggatctgat
    22081 attatgatgg catttgatga atgtccaccg atgcctgctg aatatgatta tgtaaaaaaa
    22141 tctattgaac gtacaacacg ttgggcgaaa agatgtctag atgcacacca aagacctgaa
    22201 gatcaagcat tgttcggcat tatacaaggt ggcgaatatg aagatttaag agaacaaagt
    22261 gcaaaggatt tagtagaatt agattttcct ggttatgcaa tcggcggttt gtcagttggt
    22321 gaacctaaac cggttatgta taaaatggtt gaacatacag agcagtttat gcctaaagat
    22381 aaaccaagat atttaatggg tgtaggatct ccagatgcgt taatcgaatg tagtattcgc
    22441 ggcatggata tgtttgattg tgtcttaccg acacgtattg ccagaaatgg tacttgtatg
    22501 acatcgcaag gtcgtttagt tattaaaaat gcaaaatttg cagatgattt aagaccgtta
    22561 gatgagaatt gtgactgtta tacatgtcaa aactattcaa gagcgtatat acgtcattta
    22621 atcaaggcag aggaaacttt tggtattcgt cttactacta ttcataattt acattttctg
    22681 ctaaaattaa tggaagatat aagacaagcc attcgagaag atcgtctttt agatttcaaa
    22741 gaagaattct tcgagcaata tggattaaat gttgagaacc caaaaaactt ttaagcaaga
    22801 ggagcgtata aaatgcaatt ttcattacta atatatatag tcgtaatttt tgcggttatg
    22861 tatttcttga tgatcagacc acaacaaaaa cgtgcgaaac agcatcgtga gttgattaat
    22921 aacattcaat ctggtcaaag aattacaact attggtggta ttaaaggtac tgttaaagca
    22981 gtagatgaaa caactgttgt tattacagtt aatggtcatg gtactgaatt aactttcgaa
    23041 aaacctgcta ttaaacaagt tgacccttca taacgagaag tggtattaaa aaggatgaaa
    23101 cgaatagcgt ttcatccttt ttaagttata agtagtatgt agaatagctt atttttattc
    23161 caaaaaaaga aattgtgata cataataatt gttaaattta aattaaatat atctatgact
    23221 atttttactc cctgctctag tatgttaata tgtataaggc taataaattt tgtaagaaag
    23281 atttaacttg cgttataaaa tagtattcaa gtattctaaa tacatacgta aataatgagg
    23341 tgttcatgtg aagaaaagta gtagaataat tgcgttcttg cttttagtcg tattgttgtt
    23401 tgccggaatg gctgcaactt ataaaagtgt tataaagaat gtcaatttag gacttgattt
    23461 gcaaggtgga tttgaggtgc tttatcaagt cgatccttta aataaaggcg acaaaataga
    23521 taaaaaagcc ttacaatcaa cagcgcaaac attagaaaat cgtgttaacg ttttaggtgt
    23581 ttcggaacct aaaattcaag ttgaagaacc taatagaatt agagttcaac ttgctggtgt
    23641 aacagaccag aatgaagcac gtaaaatttt atcttctcaa gctaatttaa caattcgcga
    23701 tgcagaagat aaagtaaaat taagcggttc tgatattaaa cagggatctg ccaaacaaga
    23761 atttaaacag gaaacaaacc agcctacagt tacttttaaa gtaaaagaca aaaacaaatt
    23821 taaaaaagta acggaagaaa tctctaagaa acgtgataat gtcatggttg tttggttaga
    23881 tttcaaaaaa ggtgacagct ataaaaaaga agcacaaaag aaaaacccta aatttatttc
    23941 tgcagcaagt gttgatcaac caatcaattc tgatagcgta gaaatctcgg gtggatttaa
    24001 aggacaagaa ggcgttaaaa aagcaaaaca aatcgccgaa ttgttaaatg caggttcttt
    24061 accagttgac ttgaaagaaa tttattcaaa ctcagtaggt gcccaatttg gtcaagatgc
    24121 attagataaa actgtgtttg catcgtttat tggtgtagca ttaatttatt tattcatgct
    24181 tggattctac cgattacctg gtttagtagc gattattgca ttgacaactt atatctatct
    24241 aacgttagta gcatttaatt tcatttccgg ggttctaact ttaccaggat tagcggcgtt
    24301 ggtattaggt gtaggtatgg ctgtagatgc caatattatc atgtatgagc gtattaagga
    24361 tgaacttcga ataggtagaa cgataaagca agccttttct aaagcaaaca aaagttcatt
    24421 cctaacaatt tttgattcta acttaacaac agttatcgcc gcagcagtat tattcttctt
    24481 cggtgaaagt tcagttaaag gtttcgcgac aatgttatta ttaggtattc taatgatctt
    24541 tgttacagcc gtgttcttat caagattctt attatcatta cttgtttcat caaatatatt
    24601 caaaaatcaa ttttggttat ttggtgttaa aaagaataaa cgacatgata ttaatgaagg
    24661 tgtagatgtt catgacctta aaacttcatt cgagaaatgg aattttgtta aattagctaa
    24721 gccattaatt ggagtaagta ttttaattgt agttgtcggt ttagttattc tttatatctt
    24781 caagttaaac ttaggaattg atttctcaag tggtacacgt gtagatttcc aatctaaaca
    24841 agcaattact caacaaaagg ttgagcaagt tgtaaaagat agtggactta aagcggatca
    24901 aattcaaatc aatggtaaag ataataaagt agcaactgtt caatttaaag acgatttaac
    24961 gcgtgctcaa gataataaat tgagtgataa tattaaatcg aaattcggtg acacacctca
    25021 aattaatact gtatcaccaa ttattggaca ggaattagca aaaaatgcaa tgcttgcatt
    25081 aatctatgca tcgataggta tcatcatcta tgtatcatta cgatttgaat ggcgcatggg
    25141 tctttcatct gtattggcat tattacatga tgtattcatt atagtagcga ttttcagttt
    25201 atttagaatt gaagtagatt taacatttat cgccgctgta ttaacaattg tcggttattc
    25261 aattaatgat acaatcgtaa cgtttgaccg tgtacgtgaa aacttacaaa aggttaaagt
    25321 gattacgaca acagaacaaa ttgatgatat cgttaataga tcaattagac agacaatgac
    25381 acgttcaatt aatacagtat taacagttat tgtagtagta gttgctatac tattcttcgg
    25441 tgctcctacg atattcaact ttactttagc attatttatc ggattgattt ctggtgtatt
    25501 ctcttcaatc ttcattgccg ttccgctatg gggaataatg aaaaaacgtc agttgaaaaa
    25561 atcgccgaaa cacaaattag ttgtatataa agaaaagaaa tcgaacgatg aaaagatttt
    25621 agtttaaaat gaattaagcg gtatgtgaaa caataaagag ttcacatacc gctttttact
    25681 ttatttaaat ttaacaaaat atatataaat aagttcccgc attcattttt aaaaaatggt
    25741 ataattaaag tcttgattat tt
//
LOCUS       DAHHSC010000017        25582 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.017, whole genome shotgun sequence.
ACCESSION   DAHHSC010000017 DAHHSC010000000
VERSION     DAHHSC010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 25582)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25582)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25582
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.017"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            complement(16..108)
                     /locus_tag="NBI57_000554"
     CDS             complement(16..108)
                     /locus_tag="NBI57_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1821530.1"
                     /translation="MLKSKFPSPLSTVDISFFEIIYIFSYQDIL"
     gene            complement(446..850)
                     /locus_tag="NBI57_000555"
     CDS             complement(446..850)
                     /locus_tag="NBI57_000555"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HCD1821531.1"
                     /translation="MNTLNLDIVTPNGSVYNRDNVELVVMQTTAGEIGVMSGHIPTVA
                     ALKTGFVKVKFHDGTEYIAVSDGFVEVRKDKVSIIVQTAETAREIDVERAKLAKARAE
                     SHLENDDDNTDIHRAERALERANNRLRVAELK"
     gene            complement(870..2282)
                     /gene="atpD"
                     /locus_tag="NBI57_000556"
     CDS             complement(870..2282)
                     /gene="atpD"
                     /locus_tag="NBI57_000556"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017636767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HCD1821532.1"
                     /translation="MGIGRVTQVMGPVIDVRFEHNEVPKINNALVIDVPKEEGTIQLT
                     LEVALQLGDDVVRTIAMDSTDGVQRGMDVKDTGKEISVPVGDETLGRVFNVLGETIDL
                     KEEISDSVRRDPIHRQAPAFDELSTEVQILETGIKVVDLLAPYIKGGKIGLFGGAGVG
                     KTVLIQELINNIAQEHGGISVFAGVGERTREGNDLYFEMSDSGVIKKTAMVFGQMNEP
                     PGARMRVALSGLTMAEYFRDEQGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTTKGSVTSIQAVFVPADDYTDPAPATAFAHLDATTNLERKLTE
                     MGIYPAVDPLASTSRALEPSIVGQEHYEVARDVQSTLQKYRELQDIIAILGMDELSDE
                     DKQTVERARRIQFFLSQNFHVAEQFTGQKGSYVPVKTTVANFKDILDGKYDHIPEDAF
                     RLVGSMDDVIAKAKDMGVEV"
     gene            complement(2304..3170)
                     /locus_tag="NBI57_000557"
     CDS             complement(2304..3170)
                     /locus_tag="NBI57_000557"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500821.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HCD1821533.1"
                     /translation="MASLKEIDTRIKSTKKMKQITKAMNMVSSSKLRRAEKNTKQFTP
                     YMDKMQDAITAVAGASSNTNHPMLRPRKITRSGYLVITSDKGLAGAYSANVLKKLITD
                     IEAKHQDSSEYSIVVLGQQGVDFLKNRGYDIEYSQVDVPDQPSFKSVQALANHAIDLY
                     SEEEIDELNIYYSHYVSVLENKPTSRQVLPLSQEDSSKGHGHLSSYEFEPDKESILSV
                     ILPQYVESLIYGTILDAKASEHATRMTAMKNATDNATELIDDLSLEYNRARQAEITQQ
                     ITEIVGGSAALE"
     gene            complement(3201..4709)
                     /locus_tag="NBI57_000558"
     CDS             complement(3201..4709)
                     /locus_tag="NBI57_000558"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HCD1821534.1"
                     /translation="MAIKAEEISALLRSQIENYESEMSVTDVGTVLQIGDGIALIHGL
                     NDVMAGELVEFHNGVLGLAQNLEESNVGVVILGPYTGITEGDEVKRTGRIMEVPVGEE
                     LIGRVVNPLGQPIDGQGPINTTKTRPVEKKATGVMDRKSVDEPLQTGIKAIDALVPIG
                     RGQRELIIGDRQTGKTTIAIDTILNQKDQGTICIYVAIGQKDSTVRANVEKLRQAGAL
                     DYTIVVAASASEPSPLLYIAPYSGVTMGEEFMFNGKHVLIVYDDLTKQAAAYRELSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLNDDLGGGSITALPIIETQAGDISAYVPTNV
                     ISITDGQIFLQSDLFFSGVRPAINAGQSVSRVGGSAQIKAMKKVAGTLRLDLASYREL
                     ESFAQFGSDLDEFTASKLERGKRTVEVLKQDQNKPLPVEHQVLIIYALTKGYLDDIPV
                     VDITRFEDELNHWAESNATELLNEIRETGGLPDAEKFDTAINEFKKSFSKSE"
     gene            complement(4731..5270)
                     /locus_tag="NBI57_000559"
     CDS             complement(4731..5270)
                     /locus_tag="NBI57_000559"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HCD1821535.1"
                     /translation="MVKVANKYAKALFDVSLDTNNLETINEELTVINEAVKDKIEQLK
                     MVDSNPTQTAEQRRELINGVFTDINPYIKNMMYVLADNRHISLIADVFKAFQSLYNGH
                     YNQDFATIESTYELSQEELDKIVKLVTQQTKLSKVIVDTKINPDLIGGFRVKVGTTVL
                     DGSVRNDLVQLQRKFRRVN"
     gene            complement(5270..5791)
                     /locus_tag="NBI57_000560"
     CDS             complement(5270..5791)
                     /locus_tag="NBI57_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HCD1821536.1"
                     /translation="MTETANLFVLGAAGGVEWGTVIVQVLTFIVLLALLKKFAWGPLK
                     DVMDKRERDINRDIDDAEQAKLNAQKLEEENKQKLKETQEEVQKILEDAKVQARQQQE
                     QIIHEANVRANGMIETAQSEINSQKERAIADINNQVSELSVLIASKVLRKEISEQDQK
                     ALVDKYLKEAGDK"
     gene            complement(5990..6202)
                     /gene="atpE"
                     /locus_tag="NBI57_000561"
     CDS             complement(5990..6202)
                     /gene="atpE"
                     /locus_tag="NBI57_000561"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HCD1821537.1"
                     /translation="MNLIAAAIAIGLSALGAGIGNGLIVSRTVEGVARQPEARGQLMG
                     IMFIGVGLVEALPIIGVVIAFMTFAG"
     gene            complement(6245..6973)
                     /gene="atpB"
                     /locus_tag="NBI57_000562"
     CDS             complement(6245..6973)
                     /gene="atpB"
                     /locus_tag="NBI57_000562"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HCD1821538.1"
                     /translation="MDHKSPLVSWNLFGFDIVFNLSSILMILVTAFLVFLLAIICTRN
                     LKKRPTGKQNFVEWIFDFVRGIIEGNMAWKKGGQFHFLAVTLILYIFIANMLGLPFSI
                     VTKDHTLWWKSPTADATVTLTLSTTIILLTHFYGIKMRGTKQYLKGYVQPFWPLAIIN
                     VFEEFTSTLTLGLRLYGNIFAGEILLTLLAGLFFNEPAWGWIISIPGLIVWQAFSIFV
                     GTIQAYIFIMLSMVYMSHKVADEH"
     gene            complement(6994..7446)
                     /locus_tag="NBI57_000563"
     CDS             complement(6994..7446)
                     /locus_tag="NBI57_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase subunit I"
                     /protein_id="HCD1821539.1"
                     /translation="MNRIDTTNRLYYHLVCMSGTVFMIDISHTEAYKMSRFYNIFKQF
                     IQYYFYLIIILGGLYLYTHHAFILGLIIGVTGSAINTCIFECYLAKAKRPDTMHISTG
                     NMWRYLVAIIACMIWYLNKAHVSIIGIIIGLMISYVVVIIRPLLKVSK"
     gene            complement(7514..8641)
                     /gene="wecB"
                     /locus_tag="NBI57_000564"
     CDS             complement(7514..8641)
                     /gene="wecB"
                     /locus_tag="NBI57_000564"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HCD1821540.1"
                     /translation="MKKIMTIFGTRPEAIKMAPLVKALEQEKMLEPIVVVTAQHREML
                     DSVLSTFEIKPKYDLNIMKSGQTLSEITSKSITQLEQVIQLEKPDMVLVHGDTMTTFA
                     GGLAAFYNQVPIGHVEAGLRSYDKYSPFPEEVNRQLVGVLADLHFAPTKNAASHLLSE
                     GKYSESVVVTGNTAIDAMKYTVDDNYKSNIMDKYHDKKFILMTAHRRENIGKPMENIF
                     KAVRRLIDEYTDLALVYPMHKNPKVREVAQKILGSHDRIELIEPLDVVDFHNFAKKSY
                     FILTDSGGIQEEAPSFNKPVLVLRSVTERPEGVEAGTLKVIGTNKQNVYQAAKELIDD
                     ERLYHQMSEASNPYGDGFASERIVNHIKYYLNLITEKPSDF"
     gene            complement(8665..9294)
                     /gene="upp"
                     /locus_tag="NBI57_000565"
     CDS             complement(8665..9294)
                     /gene="upp"
                     /locus_tag="NBI57_000565"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017636774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HCD1821541.1"
                     /translation="MSKVHVFDHPLIQHKLSYIRDVNTGTKEFRELVDEVGMLMAYEV
                     TRDLELQDVDIETPVTKMTAKRLAGKKLAIVPILRAGLGMTDGILSLVPAARVGHIGL
                     YRDPETLKAVEYFAKLPQDITERQIIVVDPMLATGASAIEAITSLKKRGAKNIRFMCL
                     IAAPEGVEKMHEAHPDVDIYIAALDEKLNDKAYITPGLGDAGDRLFGTK"
     gene            complement(9322..10560)
                     /locus_tag="NBI57_000566"
     CDS             complement(9322..10560)
                     /locus_tag="NBI57_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HCD1821542.1"
                     /translation="MSYITKQDKVIAEAIEREFQRQNSNIELIASENFVSEAVMEAQG
                     SVLTNKYAEGYPGRRYYGGCEFVDVTESIAIDRAKALFGAEHVNVQPHSGSQANMAVY
                     LVALEMGDTVLGMNLSHGGHLTHGAPVNFSGKFYNFVEYGVDKDTERINYDEVRKLAL
                     EHKPKLIVAGASAYSRTIDFKKFKEIADEVNAKLMVDMAHIAGLVAAGLHPNPVEYAD
                     FVTTTTHKTLRGPRGGMILCKEEYKKDIDKTIFPGIQGGPLEHVIAAKAVAFGEALEN
                     NFKTYQQQVVKNAKVLAEALINEGFRIVSGGTDNHLVAVDVKGSIGLTGKEAEETLDS
                     VGITCNKNTIPFDQEKPFVTSGIRLGTPAATTRGFDEKAFEEVAKIISLALKNSKDEE
                     KLQQAKERVAKLTAEYPLYQ"
     gene            complement(10587..11111)
                     /locus_tag="NBI57_000567"
     CDS             complement(10587..11111)
                     /locus_tag="NBI57_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HCD1821543.1"
                     /translation="MKDLTMLLDELKDMSFFNKGDICLIGCSTSEVIGEKIGTVGSME
                     VAETIFNALDVVSKETGVTFAFQGCEHINRAITIEKSQYNPLTMEEVSVVPDVHAGGS
                     LATYAFQHMKDPIVVEHITVPCGIDIGQTLIGMHIKHVCVPVRTSVKQVGQAIVTIAT
                     SRPKKIGGERAKYK"
     gene            complement(11218..11637)
                     /locus_tag="NBI57_000568"
     CDS             complement(11218..11637)
                     /locus_tag="NBI57_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight protein arginine
                     phosphatase"
                     /protein_id="HCD1821544.1"
                     /translation="MKILFVCTGNTCRSPLAESIAKEVMPNHQFESRGIFAVNNQGVS
                     NYVEDLVEEHHLAETTLSQQFTEADLKADIILTMSYSHKELIEAHFGLQNHVFTLHEY
                     VKEAGEVIDPYGGTKEMYVHTYEELVSLILKLKDIIC"
     gene            complement(11634..12680)
                     /locus_tag="NBI57_000569"
     CDS             complement(11634..12680)
                     /locus_tag="NBI57_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006190783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HCD1821545.1"
                     /translation="MDTKIWDVREYNEDLQQYPKINEIKDIVLNGGLIGLPTETVYGL
                     AANATDEEAVAKIYEAKGRPSDNPLIVHIHSKVQLKDFTYTLDPRVEKLMQAFWPGPI
                     SFILPLKPGYLCRKVSGGLSSVAVRMPSHSVGRQLLQIINEPLAAPSANLSGRPSPTT
                     FNHVYQDLNARIDGIVQAEQSEEGLESTVLDCTSFPYKIARPGSITAAMITEILPNSI
                     AHADYNDTEQPIAPGMKYKHYSPNTPLTIITDIESKIGNDGKDWSSIAFIVPSNKVAF
                     IPSEAQFIQLCQDDNDVKQASHNLYDVLHSLDENENISAAYIYGFELNDNTEAIMNRM
                     LKAAGNHIIKGCEL"
     gene            complement(12764..13600)
                     /gene="prmC"
                     /locus_tag="NBI57_000570"
     CDS             complement(12764..13600)
                     /gene="prmC"
                     /locus_tag="NBI57_000570"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HCD1821546.1"
                     /translation="MVNYKEKLDEAIHLTQQKGFEQTRAEWLMLDVFQWTRTDFVVHM
                     HDDMPKAMIMKFDLALQRMLLGEPIQYIVGFASFYGRTFDVNSNCLIPRPETEEVMLH
                     FLQQLEDDATIVDIGTGSGVLAITLKCEKPDLNVIATDISLEAMNMARNNAEKHQSQI
                     QFLTGDALKPLIKEGIKLNGLISNPPYIDEKDMVTMSPTVTRFEPHQALFADNHGYAI
                     YESIIGDLPHVMEKGSPVVFEIGYNQGEALKSIILNKFPDKKIDIIKDINGHDRIVSF
                     KW"
     gene            complement(13587..14663)
                     /gene="prfA"
                     /locus_tag="NBI57_000571"
     CDS             complement(13587..14663)
                     /gene="prfA"
                     /locus_tag="NBI57_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015445905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HCD1821547.1"
                     /translation="MFDQLDIVEERYEQLNELLSDPDVVNDSDKLRKYSKEQADLQKT
                     VDVYRNYKAKKEELADIEEMLSETDDKEEVEMLKEESNGIKAELPNLEEELKILLIPK
                     DPNDDKDVIVEIRAAAGGDEAAIFAGDLMRMYSKYAESQGFKTEIVEASESDHGGYKE
                     ISFSVSGNGAYSKLKFENGAHRVQRVPETESGGRIHTSTATVAVLPEVEDVEIEIRNE
                     DLKIDTYRSSGAGGQHVNTTDSAVRITHLPTGVIATSSEKSQIQNREKAMKVLKARLY
                     DMKVQEEQQKYASQRKSAVGTGDRSERIRTYNYPQSRVTDHRIGLTLQKLGQIMEGHL
                     EEIIDALTLSEQTDKLKELNNGEL"
     gene            complement(14664..15263)
                     /locus_tag="NBI57_000572"
     CDS             complement(14664..15263)
                     /locus_tag="NBI57_000572"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HCD1821548.1"
                     /translation="MYETYHSGWIECITGSMFSGKSEELIRRLRRGIYAKQKVVVFKP
                     AIDDRYHKEKVVSHNGNAIEAINISKASEIMTHNLTNVDVIGIDEVQFFDDEIVSIVE
                     KLSADGHRVIVAGLDMDFRGEPFEPMPKLMAVSEQVTKLQAVCAVCGSSSSRTQRLIN
                     GKPAKIDDPIILVGANESYEPRCRAHHIVAPSDNNKEEL"
     gene            complement(15610..15864)
                     /locus_tag="NBI57_000573"
     CDS             complement(15610..15864)
                     /locus_tag="NBI57_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HCD1821549.1"
                     /translation="MKQGIHPEYHQVIFLDTTTNFKFLSGSTKTSSEMMEWEDGKEYP
                     VIRLDISSDSHPFYTGRQKFAAADGRVERFNKKFGLKSNN"
     gene            complement(15982..17298)
                     /gene="rho"
                     /locus_tag="NBI57_000574"
     CDS             complement(15982..17298)
                     /gene="rho"
                     /locus_tag="NBI57_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HCD1821550.1"
                     /translation="MPERERTSPQYESFHELYKNYTTKELTQKAKTLKLTNYSKLNKK
                     ELVLAIMEAQMEKDGNYYMEGILDDIQPDGYGFLRTVNYSKGEKDIYISASQIRRFEI
                     KRGDKVTGKVRKPKDNEKYYGLLQVDFVNDHNAEEVKKRPHFQALTPLYPDERIKLET
                     ETQNYSTRIMDLVTPIGLGQRGLIVAPPKAGKTSLLKEIANAISTNKPDAKLFILLVG
                     ERPEEVTDLERSVEAAEVVHSTFDEPPEHHVKVAELLLERAKRLVEIGEDVIILMDSI
                     TRLARAYNLVIPPSGRTLSGGLDPASLHKPKAFFGAARNIEAGGSLTILATALVDTGS
                     RMDDMIYEEFKGTGNMELHLDRKLSERRIFPAIDIGRSSTRKEELLISKSELDTLWQL
                     RNLFTDSTDFTERFIRKLKRSKNNEDFFKQLQKSAEESTKTGRPII"
     gene            complement(17545..18972)
                     /locus_tag="NBI57_000575"
     CDS             complement(17545..18972)
                     /locus_tag="NBI57_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HCD1821551.1"
                     /translation="MRDYTKQYINGEWVESNSNETIEVINPATEEVIGKVAKGNKADV
                     DKAVEAADDVYLEFRHTSVKERQALLDKIVKEYENRKDDIVQAITDELGAPLSLSERV
                     HYQMGLNHFVAARDALDNYEFEERRGDDLVVKEAIGVSGLITPWNFPTNQTSLKLAAA
                     FAAGSPVVLKPSEETPFAAVILAEIFDKVGVPKGVFNLVNGDGAGVGNPLSEHPKVRM
                     MSFTGSGPTGSKIMEKAAKDFKKVSLELGGKSPYIVLDDVDIKEAAKATTGKVVNNTG
                     QVCTAGTRVLVPNKIKDAFLAELKEQFSQVRVGNPREDGTQVGPIISKKQFDQVQNYI
                     NKGIEEGAELFYGGPGKPEGLEKGYFARPTIFINVDNQMTIAQEEIFGPVMSVITYND
                     LDEAIQIANDTKYGLAGYVIGKDKETLHKVARSIEAGTVEINEAGRKPDLPFGGYKQS
                     GLGREWGDYGIEEFLEVKSIAGYFK"
     gene            complement(19210..19545)
                     /locus_tag="NBI57_000576"
     CDS             complement(19210..19545)
                     /locus_tag="NBI57_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCD1821552.1"
                     /translation="MEVCPYLEETFKILGRSWNGLIINYLSRCNDCSAHFSDMKRDLK
                     TITPRALSLKLSELAQWELVEKQIISTSPVQIIYVLTEKGKALAEALHPIEAWAQSYV
                     DLTDQRTAK"
     gene            complement(19634..20893)
                     /locus_tag="NBI57_000577"
     CDS             complement(19634..20893)
                     /locus_tag="NBI57_000577"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HCD1821553.1"
                     /translation="MAQEVIKIRGGRTLNGEVNISGAKNSAVAIIPATLLAQGHVKLE
                     GLPQISDVKTLVSLLEDLNIKASLNGTELEVDTTEIQNAALPNNKVESLRASYYMMGA
                     MLGRFKKCVIGLPGGCPLGPRPIDQHIKGFKALGAEIDESSTTSMKIEAKELKGAHIF
                     LDMVSVGATINIMLAAVYATGQTVIENAAKEPEVVDVANFLTSMGANIKGAGTSTIKI
                     NGVKELHGSEYQVIPDRIEAGTYMCIAAACGENVILNNIVPKHVETLTAKFSELGVNV
                     DVRDERIRINNNAPYQFVDIKTLVYPGFATDLQQPITPLLFMANGPSFVTDTIYPERF
                     KHVEELKRMGANIEVDEGTATIKPSTLHGAEVYASDLRAGACLIIAGLIAEGVTTIYN
                     VKHIYRGYTDIVEHLKALGADIWTETV"
     gene            complement(21359..22219)
                     /locus_tag="NBI57_000578"
     CDS             complement(21359..22219)
                     /locus_tag="NBI57_000578"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002468309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase"
                     /protein_id="HCD1821554.1"
                     /translation="MPLVSMKEMLIDAKENGYAVGQYNINNLEFTQAILEASQEENAP
                     VILGVSEGAARYMSGFYTIVKMVEGLMHDLNITIPVAIHLDHGSSFEKCKEAIDAGFT
                     SVMIDASHSPFEENVATTKKVVEYAHEKGVSVEAELGTVGGQEDDVVADGIIYADPKE
                     CQELVEKTGIDALAPALGSVHGPYKGEPKLGFKEMEEIGLSTGLPLVLHGGTGIPTKD
                     IQKAIPFGTAKINVNTENQIASAKAVRDVLNNDKEVYDPRKYLGPAREAIKETVKGKI
                     KEFGTSNRAK"
     gene            22437..22958
                     /locus_tag="NBI57_000579"
     CDS             22437..22958
                     /locus_tag="NBI57_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2529 domain-containing protein"
                     /protein_id="HCD1821555.1"
                     /translation="MSKILNTQLIGIFNRLEKQSLEIQMAAQCLIQAIGGEGYVYVKG
                     YDDLQFFESFILHSDERLKSSRKLDAIKDFKEIDSTDRVLLFAPFYNDQVALDIQKLI
                     DLDIDVVLISNKPKTDDFPDHLVHFIDLSTPRPIVYTEDYDKIVQPHAIALNYVYYDI
                     YTQMIEMTRDLEL"
     gene            complement(23067..24677)
                     /locus_tag="NBI57_000580"
     CDS             complement(23067..24677)
                     /locus_tag="NBI57_000580"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HCD1821556.1"
                     /translation="MTKFIFVTGGVVSSLGKGITASSLGRLLKDRGLNVTIQKFDPYL
                     NVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKFSNVTAGKVYSHVLKKE
                     RRGDYLGGTVQVIPHITNEIKERLLLAGESTNADVVITEIGGTTGDIESLPFIEAIRQ
                     IRSDLGRENVMYVHCTLLPYIKAAGEMKTKPTQHSVKELRGLGIQPDLIVVRTEYEMT
                     QDLKDKIALFCDINKESVIECRDADSLYEIPLQLSQQNMDDIVIKRLQLNAKYETQLD
                     EWKQLLDIVNNLDGKITIGLVGKYVSLQDAYLSVVESLKHAGYPFAKDIDIRWIDSSE
                     VTDENAAEYLADVDGILVPGGFGFRASEGKISAIKYARENNVPFFGICLGMQLATVEF
                     SRNVLGLEGAHSAELDPATPYPIIDLLPEQKDIEDLGGTLRLGLYPCSIKEGTLAQDV
                     YGKAEIEERHRHRYEFNNDYREQLEANGMVISGTSPDGRLVEMVEIPTNDFFIACQFH
                     PEFLSRPNRPHPIFKSFIEASLKYQQNK"
     gene            complement(25013..25543)
                     /locus_tag="NBI57_000581"
     CDS             complement(25013..25543)
                     /locus_tag="NBI57_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HCD1821557.1"
                     /translation="MKIQDYTKQMVDEKSFIDMAYTLLNDKGETMNLYDIIDEFRALG
                     DYEYEEIENRVVQFYTDLNTDGRFLNVGENLWGLRDWYSVDDIEEKIAPTIQKFDILD
                     ADDEEDQNLKLLGEDEMDDDDDIPAQTDDQEELNDPEDEQVEEEINHSDIVIEEDEDE
                     LDEDEEVFEDEEDFND"
BASE COUNT         7656 a         5074 c         3726 g         9126 t
ORIGIN      
        1 acataaatat cataactata aaatatcttg gtatgaaaaa atgtagataa tttcaaaaaa
       61 tgaaatgtcg actgtagata gtggtgaagg aaattttgat tttagcaatc tattagtgat
      121 tgtaaaaaag taaaactatt cttgggagtc ggacataaat caatgtctag actctatagt
      181 gttgtatttg gcagtagttg acggggcccc aacataaaga aatacttttt ctttaaaaat
      241 tagtatttct tatacatgag ttttactcat gtattcctat ttttaaatac acattagctg
      301 tgtttaatga taaagaacca ctacatcata aatctttaat ggttctttat caattccttc
      361 ccccctctca tgttttgtat ctttaaatca atataattca aaaaagggtc gaagatatga
      421 aatcacatct tcgacccttt atttactatt ttaattcagc cacacgcaaa cggttatttg
      481 ctctctctaa agctctttcg gctctatgaa tatcagtatt gtcgtcatca ttttccaagt
      541 gagactctgc tcttgctttg gctaatttag ctctttcaac atcaatttct cttgcagttt
      601 ctgcagtctg aacaatgatt gaaactttat cttttctaac ttcaacaaag ccatcgctta
      661 cagcaatata ttcagttcca tcgtgaaatt tcacttttac aaagcctgtt tttaaagcag
      721 ctacagttgg aatatgtcca ctcatgacac ctatctcacc agctgttgtt tgcataacaa
      781 cgagttcaac attatcacga ttgtaaacag aaccattagg agtgacaata tctaggttta
      841 atgtattcat tatccattcc tcctaattgt tatacttcaa cacccatatc tttagctttt
      901 gcaataacat catccatgct accaactaaa cggaatgcat cttctggaat atggtcatat
      961 ttaccatcta agatatcttt aaagtttgca actgttgtct taacaggtac ataagaacct
     1021 ttttgaccag taaattgttc cgctacgtgg aagttttgag ataagaagaa ttgaattcta
     1081 cgtgcgcgtt caactgtttg tttatcttca tcagataatt cgtccatacc taagatagca
     1141 atgatatctt gcaattcacg gtatttttga agtgttgatt gtacatcacg agctacttca
     1201 taatgttctt gacctacaat tgatggttcc aatgctcttg atgtagacgc taatggatcc
     1261 acggctggat aaatacccat ttcagttaat ttacgttcta agttagtagt tgcatctaaa
     1321 tgggcaaacg ctgtcgcagg cgctgggtca gtatagtcat cggcaggtac gaataccgct
     1381 tgaatagaag taactgatcc ttttgttgta gacgtaatac gttcttgtaa ttgtcccatt
     1441 tcagtagcaa gtgttggttg gtaacctact gcagaaggca tacgacctaa taatgcagat
     1501 acctcagaac cagcttgtgt aaatctgaaa atgttatcga tgaataataa tacgtcttga
     1561 ccttgttcgt cacggaaata ttcagccatt gttaaaccag ataatgcaac acgcatacgt
     1621 gcaccaggtg gctcattcat ttgcccgaat accatggctg ttttcttaat tacaccactg
     1681 tcactcattt cgaagtataa atcgttacct tcacgagtac gttcacctac accggcgaat
     1741 acagaaatac caccgtgctc ttgagcgatg ttgttaatta attcttggat taatactgtt
     1801 ttacctacac cggcaccacc gaacaatccg attttaccac ctttaatata aggtgctagt
     1861 aaatctacta ctttaatacc tgtttctaaa atttgaactt ctgttgaaag ttcatcgaat
     1921 gctggtgctt gacgatggat aggatcgcgg cgaacagaat cactaatttc ttctttaagg
     1981 tcaattgttt cacctagtac attaaataca cgacctaatg tttcgtcacc aacaggtaca
     2041 ctaatttctt tgcctgtatc ttttacatcc atgcctcttt ggacaccatc agttgaatcc
     2101 atcgcaattg tacgaacaac gtcgtcacct aattgcagcg caacttctaa tgttagttgt
     2161 attgtacctt cttctttagg cacatcaata accaaggcgt tattaatttt aggaacttcg
     2221 ttatgttcaa atcgaacatc aattacagga cccataactt gagttacacg gccaattccc
     2281 atgctatttt cctcctttaa atattattca agcgctgcgg aaccaccaac aatttcagta
     2341 atttgttgcg taatttctgc ttgtctcgct ctgttatatt ctaatgataa gtcatcaata
     2401 agttcagttg cattatcagt ggcatttttc atcgcagtca tacgtgttgc atgctcactt
     2461 gcttttgcgt ctaatattgt tccgtaaatc aaactctcaa catattgagg caagattaca
     2521 cttaagatag attctttatc tggctcaaat tcataagaag acaaatgacc atgcccctta
     2581 ctagaatcct cttgagataa tggtaatact tgtctagatg taggcttgtt ttcaagaacg
     2641 ctgacataat gactatagta tatatttaat tcatcaattt cttcttcact gtataagtct
     2701 atagcatggt tagctagtgc ttgaacagat ttgaaagaag gttgatcagg tacgtctact
     2761 tgagaatact caatgtcata acctctattt tttaggaaat caacaccttg ttgccctaaa
     2821 actacaatac tgtattcgct actatcttga tgtttcgctt caatatcagt aatcaatttt
     2881 ttaagcacgt ttgcactata tgcacctgct aaacctttat cactcgtgat aactaaatat
     2941 ccacttctag taattttacg aggtcttagc attggatggt ttgtattgct acttgcccct
     3001 gcaactgcag taattgcatc ttgcatttta tccatatatg gtgtgaattg ttttgtattt
     3061 ttttcagctc tacgaagttt tgaacttgat accatgttca tcgctttcgt aatctgcttc
     3121 atttttttgg ttgattttat tcgagtatct atttctttaa gagaagccac tatctcacca
     3181 ccttatacta aacttgttta ttattcagat ttgctaaagc tttttttgaa ttcgttaatt
     3241 gctgtgtcaa acttctcagc atctggtaag ccaccagttt ctctgatttc atttaacagt
     3301 tcagtagcat ttgattctgc ccagtggttt aactcgtctt caaaacgtgt gatatctaca
     3361 actggaatat catctaaata tccttttgtt aatgcataaa taatcaacac ttggtgttcg
     3421 actggtaatg gtttgttttg atcttgtttt aagacttcaa cggtacgttt accacgttct
     3481 aatttacttg cagtaaattc atcaaggtct gaaccgaatt gtgcaaatga ttcaagttct
     3541 ctgtatgacg ctaagtcaag acgtaacgta ccagcaactt tcttcattgc tttaatttgt
     3601 gcagatccac caacacgaga tacagattgt ccggcattaa tcgctggtct tacacctgag
     3661 aagaataaat cagattgtaa gaagatttgt ccatctgtaa ttgaaataac gtttgttggt
     3721 acataagctg aaatatcacc agcttgtgtt tcgataattg gtaatgcagt aattgaaccg
     3781 ccacctaagt catcgtttaa ttttgctgct ctttctaata atctactatg taagtagaat
     3841 acgtcacctg ggtatgcttc acgacctgga ggtctacgta ataataatga taactcacga
     3901 taagctgctg cttgtttagt taaatcatca taaacgatta aaacatgttt accgttaaac
     3961 atgaattctt cacccattgt tacacctgaa tatggtgcaa tatataataa tggagaaggt
     4021 tcagaagctg atgctgctac aacaatagtg tagtctaaag cgcctgcttg tcttaacttt
     4081 tcaacatttg ctcttactgt tgaatctttt tgaccaatag caacatagat acaaatcgta
     4141 ccttgatctt tttggttcaa aattgtgtca attgcaattg ttgttttacc tgtttgacgg
     4201 tcaccaatga ttaactcacg ttgacctcta ccaattggta ctaaagcatc aattgctttg
     4261 atacctgttt gtaatggctc atctactgat ttacgatcca ttacaccagt agcttttttc
     4321 tctactggac gtgttttagt tgtgttaatc ggtccttgtc catcaatagg ttgtcctaat
     4381 ggattaacaa ctcttccgat tagttcttca cctactggta cttccatgat acgaccagta
     4441 cgtttaactt cgtcaccttc agtaatacct gtatatggtc ctaaaataac cacacccacg
     4501 tttgactctt caaggttttg ggctaaacca agtacgccgt tatggaattc tactagctca
     4561 ccagccataa cgtcatttaa tccgtgaatt aatgcaatac catcaccaat ttgtaataca
     4621 gtacctacat cagttacgga catttctgac tcataatttt caatttgtga gcgaagtaat
     4681 gcactgattt cttcagcttt tatggccatc tatgtcactc ctctttataa ttaattaact
     4741 cttctaaatt ttctttgtaa ttggacaaga tcatttctaa cactaccatc taatacagtt
     4801 gtgccgactt taactctaaa tccaccaatt aaatctggat taatttttgt atctacaata
     4861 actttagata acttcgtttg ttgagttact agtttgacaa tcttatctaa ctcttcttga
     4921 ctcaattcat atgttgactc aattgttgca aaatcttgat tgtagtgtcc gttatataag
     4981 ctttggaacg ccttgaatac gtcagctatt aatgagatat gtctattatc tgctaacacg
     5041 tacatcatat ttttaatgta tggattaatg tcagtaaata caccattaat caactcacga
     5101 cgttgttctg ctgtttgagt tggattactg tcaaccattt tcaattgctc aattttatct
     5161 tttactgctt catttataac tgttaattct tcattaatag tctctaaatt atttgtatct
     5221 aatgacacgt caaataatgc tttagcatac ttgttagcta cttttaccat tatttatcgc
     5281 ctgcctcttt tagatacttg tcaaccaatg ctttttggtc ttgttcagaa atttcttttc
     5341 taagaacttt agaagcaatt aacactgata gttcagatac ttgattatta atatctgcaa
     5401 tggcacgttc tttttggcta ttgatttcac tttgtgctgt ttcaatcata ccgtttgcac
     5461 gtacgtttgc ttcatgaata atttgttctt gctgttgacg tgcttgaacc ttagcatctt
     5521 ctaaaatctt ttgaacttct tcttgtgttt ctttaagttt ttgtttattt tcttcttcaa
     5581 gtttctgtgc atttaactta gcttgttctg cgtcatcgat atctctgtta atatctcttt
     5641 cacgtttatc cattacatct ttcaatggac cccatgcgaa ctttttaagt aacgcaagta
     5701 acacgatgaa agttaggacc tgtacaatca cagtacccca ctcaacgcct ccagctgcac
     5761 caagaacgaa taagttagct gtttcagtca cgggttcatg acactccttt cattaaaata
     5821 atcgctagtg ttatatagaa aaaaaacgac gaggttagga cctactgaat ttatgcgata
     5881 ggcatggtgc attcatatct caactattta ataaagttca aagatattac tatatatgca
     5941 tcatgaatta tgtcctatgc gctttgtccc gacctctttt atctgttaat tatccagcaa
     6001 atgtcatgaa tgcaattact acaccgatga taggtaatgc ctcaactaaa cctacaccaa
     6061 tgaacatgat acccattaat tgaccacgtg cttctggttg acgtgctaca ccttcaactg
     6121 ttcttgaaac gattaaaccg ttaccgatac ctgctcctaa tgctgataaa ccaattgcga
     6181 ttgctgctgc gattaaattc ataatttaat ttcctcctgt gattgtatat aattattgaa
     6241 atttttagtg ttcatctgcc actttatgtg acatataaac catcgaaagc ataataaaga
     6301 tatatgcttg gattgttcct acaaatattg aaaatgcttg ccaaacaatt aatcctggga
     6361 tactaataat ccaaccccat gctggttcgt taaagaataa gccagcaagt aatgttaata
     6421 gtatctcacc tgcaaatatg ttaccgtaca aacgcagacc aagcgttaat gttgaagtga
     6481 actcttcaaa aacattaata attgccaatg gccaaaacgg ctgtacataa cctttaagat
     6541 attgtttcgt accacgcatt ttaattccat aaaagtgagt taacagtatt atcgttgtag
     6601 acaacgttaa agtcactgtt gcatcagctg tcggtgattt ccaccacaat gtgtgatctt
     6661 tcgttactat agaaaacgga agacctaaca tattagctat aaaaatgtac agaatcagcg
     6721 ttactgctaa gaagtggaat tgaccacctt ttttccaagc catgttacct tcaatgattc
     6781 ccctcacgaa atcaaaaatc cattcaacga aattttgttt gccagttggt ctttttttca
     6841 aattacgcgt acaaatgata gcaagtagaa aaacaagaaa cgccgtaaca agtatcatca
     6901 atatacttga taaattgaaa acgatatcaa aaccgaataa attccaactc acgagcgggg
     6961 atttgtgatc cataatctca cctctttctt aatttatttg ctcaccttta gtaaaggacg
     7021 tatgataact acaacatatg aaatcattaa accaataatt ataccgatga tacttacatg
     7081 cgctttatta aggtaccaaa tcatacaggc aataattgca actaagtatc gccacatatt
     7141 tccagttgaa atatgcatag tgtctggtct tttagcttta gctaaatagc attcaaaaat
     7201 acatgtgttg attgcagaac cagtaacacc aatgattaat cctaagataa atgcatggtg
     7261 tgtataaagg tataatcctc ccaatattat tattagataa aaataatatt gaatgaactg
     7321 tttaaaaatg ttgtagaaac gactcatttt atatgcctcc gtgtgactga tatcaatcat
     7381 gaaaaccgtt ccactcatac aaactaaatg ataatatagg cgatttgtgg tgtcaattct
     7441 attcaaattg ttgtaaaaaa ataagcatag ttagacacaa tttagacaca attagacaaa
     7501 aagtctattt tcctcagaaa tcgcttggtt tttcagtgat taaattcaaa taatacttaa
     7561 tatgattaac tattctttct gaagcaaatc catcaccgta aggattagat gcttcagaca
     7621 tttgatgata taatctctca tcatcgatta attcttttgc agcttgatac acattttgct
     7681 tatttgttcc aatgactttt aatgtgccag cttcaacacc ttcaggacgt tctgtaacgc
     7741 ttcgcaaaac taaaactggc ttattaaatg atggcgcttc ttcctgaatt ccacctgaat
     7801 ctgtcaaaat aaaataagat tttttagcaa aattatggaa atctactacg tccaaaggtt
     7861 caatcaattc aattctgtca tgactaccta aaatcttttg agccacctct cgaactttcg
     7921 ggtttttatg cattggatat accagtgcta aatcagtata ctcatctatt aagcgtctaa
     7981 ccgctttaaa tatattttcc atgggtttcc cgatattttc tcgtcggtgt gctgtcataa
     8041 gaatgaattt tttgtcatgg tatttatcca tgatgttaga tttataattg tcatcaactg
     8101 tatatttcat agcatcaatc gcagtattac cagtgacaac aacactttct gaatatttcc
     8161 cttcacttaa caaatgcgat gcagcatttt tagtaggtgc aaaatgtaag tcagctaata
     8221 caccaactaa ttgtctattc acctcttctg gaaaaggtga atatttatca taacttctaa
     8281 gccctgcttc aacgtgtcca atcggcactt ggttataaaa tgccgctaaa ccacctgcaa
     8341 atgtcgtcat cgtatcacca tgtacaagta ccatgtctgg tttttctaat tgaatcactt
     8401 gttctaattg agtgattgat ttagaagtta tctcagaaag tgtctgtcct gatttcataa
     8461 tattcaaatc gtattttggt ttgatttcaa aggtacttaa tactgaatca agcatttctc
     8521 tatgctgtgc tgtaacaaca acaattggct cgagcatttt ttcttgttcc aaagctttaa
     8581 ctaaaggagc catctttatg gcttcgggtc ttgttccaaa tatggtcata atctttttca
     8641 tcaaactact tatctccgat tcttctattt agtaccaaac aatctatctc cagcgtcgcc
     8701 taaccctggt gtgatatatg ctttatcatt tagcttttca tcaagtgcag caatataaat
     8761 atctacatcc ggatgtgctt catgcatctt ttctacgcct tctggtgctg caattaaaca
     8821 catgaagcga atatttttag cgccacgttt cttcaatgaa gtaatagctt caattgctga
     8881 tgcgcctgtt gctaacatag gatcaacaac aatgatttgt ctttcagtaa tatcttgagg
     8941 taacttagca aaatactcta cagcctttaa tgtttcggga tctcgatata aaccgatatg
     9001 tccaactctg gctgcaggta ctaaacttaa aataccatca gtcataccta aaccagctct
     9061 taaaattgga acgatagcta attttttacc agctaatcgt ttagccgtca ttttagttac
     9121 aggcgtttca atatcaacat cctgaagctc taagtctcta gttacttcat atgccatcaa
     9181 cataccaact tcgtctacaa gttctctaaa ttctttagta cctgtattta catctctaat
     9241 atagcttagt ttgtgttgaa ttaatggatg atcgaaaacg tgtactttac tcataaaaat
     9301 tactcctatc tttgtgtatg tttattgata tagaggatat tcagctgtta atttcgcaac
     9361 gcgttcttta gcttgttgta atttttcttc atctttacta tttttcaatg ctaaactgat
     9421 gatttttgca acttcctcaa aagctttttc atcaaatcca cgcgttgttg cagcaggtgt
     9481 acctaaacgt ataccactcg ttacaaaagg tttttcttga tcgaacggaa tggtattttt
     9541 gttacatgtg ataccaactg aatctaaagt ctcttcagct tctttaccag taagtcctat
     9601 agaccctttt acatcaacag ctactaagtg attatctgta ccgccagaaa caattctaaa
     9661 tccttcatta attaatgctt ctgcaagaac ttttgcgttt ttaaccactt gttgttgata
     9721 cgttttgaaa ttattttcta acgcttctcc aaaagcaact gcttttgctg caataacatg
     9781 ctcaagaggt ccaccttgaa taccagggaa aattgtttta tctatgtctt ttttatattc
     9841 ttccttacat aaaatcatac caccacgtgg tccgcgtaat gttttgtgtg ttgtagttgt
     9901 tacaaaatca gcatattcta ctggatttgg atgtaaacct gccgctacta atcctgcaat
     9961 atgtgccatg tctaccatta acttagcgtt tacttcatct gcgatttctt taaacttttt
    10021 gaagtcaatt gttcttgaat atgctgatgc tcctgccaca ataagcttag gcttatgctc
    10081 taacgctaat ttacgaactt catcataatt gattcgttct gtgtctttat ctactccata
    10141 ttcaacgaaa ttgtagaatt taccactaaa attaacaggc gctccatgtg tcaagtgacc
    10201 accatgactc aaattcatac ctaaaactgt gtcgcccatt tctaatgcaa ctaagtaaac
    10261 agccatgttc gcttgtgaac ctgaatgtgg ttgaacattg acatgttcag ctccaaacaa
    10321 tgctttagca cgatcaattg cgatgctttc agtaacatct acaaactcac atccaccata
    10381 atatcggcgt cctggatagc cttcagcata cttattagtc aacactgaac cttgtgcttc
    10441 cataaccgct tccgatacaa aattttccga tgcgattaac tctatgttgc tattttgtct
    10501 ctgaaattct ctctcgattg cttctgcgat aactttatct tgcttggtga tataagacat
    10561 aaaatctccc cttctttcaa aaaaacttat ttgtatttag cacgttcgcc accaatcttt
    10621 ttcggcctag atgtggcaat agttacaatt gcctgtccta cttgctttac tgaggtcctt
    10681 acaggtacac atacatgttt aatatgcatg cctattaatg tttgaccaat atcaattcca
    10741 caaggaacag taatatgttc gaccacgatc ggatccttca tatgctgaaa agcgtatgtt
    10801 gccaaactcc ctccagcatg tacatctgga acgacggaaa cttcttccat tgttaatgga
    10861 ttatactgag atttttctat tgttatcgct ctgttgatat gttcacatcc ttgaaaagca
    10921 aaagtaacgc ctgtctcttt actcacaaca tctaatgcat taaaaatagt ttctgcaact
    10981 tccatcgaac cgacagtccc tattttttcg ccaatgactt ccgatgttga acatccaatt
    11041 aaacatatat ctcctttatt aaaaaaggac atatctttta attcgtctaa taacattgtc
    11101 aaatctttca taaaagccca cccttcctaa aaataaaaaa ggaatatagc aaagtgctac
    11161 actcctctat tataacttat ttaactgtta acatatacta attatacaga attcctacta
    11221 gcaaataata tcttttaatt ttaaaattaa acttacaagt tcttcatagg tatgtacata
    11281 catttctttt gttccaccgt atggatctat aacttctcct gcttctttta catattcatg
    11341 caatgtgaaa acatgatttt gcaaaccaaa gtgtgcctct attaattctt tgtgcgaata
    11401 cgacatcgtc aaaataatat ctgctttcaa atctgcttca gtaaattgtt gcgataaggt
    11461 cgtttcagct aaatgatgtt cttcaactaa gtcttcaaca taattcgaaa caccttgatt
    11521 gttcacagcg aatatacctc ttgattcaaa ttgatgattt ggcataacct cttttgcaat
    11581 actttccgct aatgggctac gacatgtgtt acctgtacaa acgaataaaa tcttcatagt
    11641 tcacatcctt taataatgtg attacctgca gcttttaaca tgcgattcat aattgcttct
    11701 gtattatcat tcagctcaaa gccgtatata tacgccgctg aaatattttc attttcatca
    11761 agtgaatgta acacatcata aagattatga cttgcttgtt taacatcatt gtcatcctga
    11821 cataattgaa tgaattgcgc ttcacttggt ataaacgcca ccttattact cggcacaata
    11881 aaagctatag aagaccaatc tttaccgtca tttccaattt tgctctcaat atctgtaata
    11941 attgtaagtg gtgtattggg tgagtaatgc ttatacttca tacctggtgc aattggctgt
    12001 tcagtatcat tataatcagc atgggcgata ctattcggaa gtatttctgt aatcattgct
    12061 gctgttatag aaccaggtct tgcaatttta taaggaaaag atgtgcaatc taaaaccgta
    12121 ctttctaatc cttcttcact ttgttcagct tgaacaatac catctatacg ggcattcaaa
    12181 tcttgatata catgattgaa agttgttggt gaaggtctac cacttaaatt agcacttgga
    12241 gcagctagag gttcatttat gatttgtaat aattgtctac ctacagaatg gcttggcatt
    12301 ctaacagcaa ctgatgataa acctccagaa acttttcgac atagatagcc tggctttagc
    12361 ggcaatataa acgaaatagg gcccggccag aatgcctgca ttaacttttc tacgcgtgga
    12421 tccaaagtat atgtaaaatc ttttaattga actttactgt gtatatgaac aataagcgga
    12481 ttgtcagatg gacggccttt agcttcatat attttagcta cagcttcttc atctgtcgca
    12541 tttgctgcaa gtccataaac tgtttcagtt ggtaaaccta ttaaaccacc gtttaaaaca
    12601 atgtctttta tttcattaat tttaggatat tgctgtaaat cttcattata ttctctaaca
    12661 tcccaaattt tagtatccaa cttaatcacg cctttcttat ttatcataat ataaagcaaa
    12721 aagctatgca cttaactaat catagcaaag gcataacttc taattaccat ttaaatgaga
    12781 cgattcgatc gtggccattt atatctttaa taatgtcgat ttttttgtca ggaaatttat
    12841 ttaaaattat tgatttaagt gcctcacctt gattgtaacc aatttcaaaa acaactgggc
    12901 tgcctttttc cataacgtga ggtaaatctc caatgattga ttcataaata gcatatccat
    12961 ggttatctgc aaacaatgcc tgatgtggtt cgaatctcgt aaccgttgga gacatcgtaa
    13021 ccatatcttt ttcatctata tatggtggat tagatatcaa gccgttcaac ttgatacctt
    13081 ctttaattaa gggctttaat gcatcccctg ttaaaaattg tatttgtgat tgatgcttct
    13141 cagcattatt acgagccata ttcattgctt caagtgaaat atcagtagca ataacattta
    13201 aatccggctt ttcacatttc aaagtaattg caagtacacc actacccgtt ccgatatcta
    13261 cgattgttgc atcatcttct aactgttgta agaaatgcaa cattacttct tcagtttcag
    13321 gtcttggtat caaacaattt gagtttacat caaacgttct accataaaat gaggcaaagc
    13381 caactatata ctgtataggc tctcctaata acatacgttg taatgctaag tcgaacttca
    13441 taatcatcgc tttcggcata tcatcatgca tgtgaactac aaagtccgta cgcgtccatt
    13501 gaaatacatc taacattaac cattcagctc gtgtttgttc aaaccctttt tgttgtgtta
    13561 aatgaattgc ttcatctaac ttttctttat aattcaccat tattaagttc tttcaattta
    13621 tctgtctgct ctgataaagt cagtgcatct ataatttctt ctaaatggcc ttccataatt
    13681 tgccctaatt tttgaagcgt tagacctata cgatggtctg ttacacggct ttgtggataa
    13741 ttataagttc gaatacgttc tgaacgatca ccagtaccga ctgctgattt acgttgtgac
    13801 gcatactttt gttgttcttc ttgaactttc atatcgtata aacgtgcttt taacactttc
    13861 attgcttttt cacggttttg aatttgagac ttctcagaag atgttgcaat gacaccagtt
    13921 ggtaaatggg taatacgtac tgcagagtca gttgtgttta cgtgctgacc acctgcacca
    13981 cttgaacgat acgtgtcgat ttttaaatct tcatttctaa tttcaatttc tacatcttca
    14041 acttctggta aaactgccac tgtagctgtt gaagtatgaa tacgtccacc tgattctgtt
    14101 tcaggcacac gttgaacgcg gtgcgcacca ttttcaaatt tcaatttact atacgcgcca
    14161 ttaccagaaa ctgagaaact aatttctttg taaccaccat ggtcactttc agacgcttct
    14221 actatttcag ttttgaatcc ttgtgattca gcatactttg aatacatacg cattaaatca
    14281 ccagcaaaaa tcgcagcctc atcaccacct gctgctgctc ttatttctac aataacgtct
    14341 ttgtcatcat taggatcttt aggaatcaat aatattttaa gctcttcttc aagatttgga
    14401 agttcagctt taataccatt actctcctct tttaacattt ctacttcttc tttatcatca
    14461 gtctcactta acatttcttc aatatcagct aattcttctt ttttagcttt atagttacga
    14521 taaacatcta cagttttttg taaatcagct tgctctttag aatatttacg taatttatct
    14581 gaatcattta caacatctgg gtcacttaac agttcattta actgttcgta tctttcttct
    14641 acaatatcta attgatcaaa cacttataat tcctccttat tattatcact aggtgctacg
    14701 atatggtgcg cgcgacaacg tggctcataa ctttcattgg cacctactaa gataatcgga
    14761 tcatcgattt tagctggttt accatttatt aatcgttgcg ttctactaga tgaagaacca
    14821 caaacagcac aaactgcttg aagtttcgtt acttgttcac tgacagccat caatttaggc
    14881 attggttcga acggttcgcc cctaaaatcc atatctaatc cagcaacaat aacacggtgt
    14941 ccatctgctg atagtttttc tactatactt acaatttcat cgtcaaaaaa ttgcacttcg
    15001 tctattccta taacatcaac attagttaag ttgtgcgtca taatttcact tgctttagaa
    15061 atattaatcg cttcaatggc attaccatta tgagagacca ctttttcttt atgatatcga
    15121 tcatcaatcg ccggtttaaa tacaacgact ttttgtttag cgtatatacc ccttcttaga
    15181 cgtcttatta gttcttcgga tttaccgcta aacatactac ctgtaataca ttctatccaa
    15241 ccggaatggt aagtttcata cattgagagt tccacctttt tcaaaacata atcgctttat
    15301 tatatcatat ttcaaatatt cataaatgtc tttttcataa ttatatcgat attgtacatg
    15361 aacaattatt ttataaattt gtcttgcata tttatataca gctattctaa tttgttgctt
    15421 cttattcgtg cggtgtttct actcaataca aactttaata aagggggaaa tttatcatta
    15481 tgtattaaca acatggtatt gagtcacttc tttgaacaca gtcgtaagta tataccctta
    15541 ctacgcttat aaataaaaaa gctgtctaca attccattat aggatgcaga cagcttaacg
    15601 attactatat tagttgtttg atttaagacc aaactttttg ttgaaacgtt ccacacgtcc
    15661 atccgcagca gcgaattttt gacgtccagt gtagaatgga tgtgaatcag atgaaatatc
    15721 taaacgaata actgggtatt cttttccatc ttcccactcc atcatttctg aagatgtttt
    15781 tgtagaaccg cttaagaatt tgaagtttgt agtagtatct aaaaagatta cttggtgata
    15841 ctcaggatga attccttgtt tcattatttt cagctccttt gccctgaacc atctggaaca
    15901 gagttatttg tgagttttta cccaatactg tgtaattata aaggtaatta attcaaaacg
    15961 caagccccta tataatgttt attaaattat aggtcgaccc gttttagtac tttcttctgc
    16021 agacttttgt agctgcttga agaaatcttc attattctta gaccttttaa gtttgcgaat
    16081 aaatctttca gtaaagtcag ttgagtcagt gaatagattt cttaattgcc ataatgtgtc
    16141 taattcagat ttacttatta acaattcttc tttacgcgtt gaacttctgc caatatcaat
    16201 tgcagggaag atacgacgtt cagacaattt acgatctaaa tgtaactcca tgttacctgt
    16261 tcctttaaat tcttcgtaaa tcatatcgtc catacgtgaa cccgtatcaa ctaatgcagt
    16321 tgcaagtatt gttaaacttc cacccgcttc aatatttctc gctgcaccga agaatgcttt
    16381 tggtttgtgt aaagatgcag gatctaaacc acctgataat gtacgaccac ttggtggaat
    16441 aactaagtta taagcgcgtg ctaatctcgt tatagaatcc attaaaataa tgacatcttc
    16501 cccaatttct actaaacgct ttgcacgttc aagtaataat tcagctactt taacatggtg
    16561 ttctggtggt tcgtcaaacg ttgaatgaac gacttcagca gcttctactg agcgttctaa
    16621 atctgtcacc tcttcaggac gctcgccaac taacaaaata aatagctttg catctggttt
    16681 gttcgtactg attgcattcg ctatttcttt taataacgat gttttacctg ctttaggtgg
    16741 cgccactatt aaaccacgtt gacctaaacc aatcggtgtt actaaatcca tgatgcgcgt
    16801 tgaataattt tgtgtttctg tctctaattt aatacgctca tctggataaa gtggtgtcaa
    16861 agcttggaaa tgcggacgtt tcttcacttc ttctgcgtta tggtcattga caaagtcaac
    16921 ttgtaataag ccataatatt tttcgttatc tttaggtttt ctaactttcc cagttacttt
    16981 atccccacgt ttaatttcaa aacgacgaat ttggctagca gatatataaa tatctttttc
    17041 ccctttagaa tagttcactg ttcttaaaaa accataacca tctggttgta tatcatctaa
    17101 gataccttcc atataatagt taccatcttt ttccatttgt gcttccataa tagctagaac
    17161 aagttctttt ttatttaatt tactatagtt cgtcaactta agagttttag ctttttgagt
    17221 gagttccttg gtagtatagt tcttgtacaa ttcgtggaat gattcatact gaggagatgt
    17281 acgttctctt tcaggcatat agtttacacc catttcatta ttttttagtt agtaagtaat
    17341 agataggcat gacaatgcat aaataactgt gctagcacaa tcccattatg ctaatcccca
    17401 accttttaaa tacatgaata actatagcaa atttttattt taattacaaa atctatttat
    17461 aaaaattgca aaattaaaac agtacacaaa acttaattag tcatgtgcac tgttttattg
    17521 cgatttttaa ttcaaaaatc aaatttattt aaaatatcca gctatagatt tcacttctaa
    17581 gaactcttca ataccataat cgccccattc acgacctaaa ccagattgtt tatagccacc
    17641 aaatggtaaa tctggctttc tacctgcttc gtttatttct actgtacctg cttcaataga
    17701 acgagctact ttatgcaatg tttctttgtc cttaccaata acatatcctg ccaaaccata
    17761 ttttgtatca tttgcaattt gaatcgcttc atctaaatcg ttataagtga taactgacat
    17821 tactggccca aaaatttctt cttgtgctat cgtcatttga ttatctacat taataaaaat
    17881 tgtcggacgt gcaaagtatc ctttttcaag tccttctggt ttaccaggac caccataaaa
    17941 taattcagca ccttcttcaa tacctttatt aatataattt tgtacttgat caaattgttt
    18001 tttactaatg atagggccta cttgtgtacc atcttctctt ggattaccga cacgcacttg
    18061 gctaaattgt tcttttaatt cagctaagaa tgcatcttta attttgttag gcactaaaac
    18121 acgtgtacca gctgtacata cttgaccagt attattaaca actttgcctg ttgttgcttt
    18181 agccgcttct ttaatatcta cgtcatctag gacgatatat ggtgatttgc caccaagctc
    18241 taatgatacc tttttaaaat ctttagcggc tttttccata attttagaac cagtagggcc
    18301 tgatcctgtg aatgacatca tgcgtacttt aggatgttca gataaaggat tcccaacacc
    18361 agcaccatca ccattaacaa ggttaaatac acctttagga acaccgactt tatcaaaaat
    18421 ctcagctaaa ataacagctg caaatggtgt ttcttcagat ggtttaagta caactggact
    18481 accagccgca aatgctgctg ctaattttaa tgatgtttgg tttgtaggga agttccacgg
    18541 tgtaattaat ccagatacac cgattgcttc tttaacaact aaatcatctc cgcggcgttc
    18601 ttcaaattcg tagttatcta atgcgtctct cgctgcaaca aaatggttta gtcccatttg
    18661 ataatggaca cgctcagata atgataaagg agcacctaat tcatccgtaa tagcttgtac
    18721 aatatcgtct tttctgtttt catattcttt tacaatttta tctaataacg cttgtctttc
    18781 tttcacagat gtatgacgga actctaaata aacatcgtct gccgcctcga cggctttatc
    18841 aacatcagct ttattacctt tagcaacttt cccgattact tcttcggttg ctggatttat
    18901 aacttctatc gtttcattac tattactttc tacccattcg ccattaatgt attgctttgt
    18961 gtagtctctc attattcttt cactcctcaa gaatgattta tatattattt atccgtaatg
    19021 catacgatat aaacattaca ttccattatg atacgctcca ttttaatatg ttcaaaataa
    19081 aatgctttaa aattttgctg catctatttt tttatatttt taaataaaaa taactattcc
    19141 cacatcatcg caaataatgc gaagacttag aaatagttat aatcaatgct atgtttaaac
    19201 gtcatgtaat tatttagcag tacgttgatc tgttaaatcg acatatgatt gcgcccatgc
    19261 ttcaattgga tgtaaagcct ctgctaacgc tttacctttt tcagttagca cataaataat
    19321 ttgtactgga ctcgtagaaa tgatttgctt ctcaactaac tcccattgtg caagctctga
    19381 caacttaaga cttaaagcac gtggtgttat tgttttcaaa tctcttttca tatcggaaaa
    19441 gtgtgctgaa cagtcattac atcttgagag ataattaatg attaatccat tccaacttct
    19501 accaagtatt ttaaaagttt cttcgagata cggacatact tccatcatct tcacctctca
    19561 tcaataaatt tagaatgtaa acgaacaccc ttttttaatc agtataatta ttataccaca
    19621 aaacaagata aatctataca gtttccgtcc aaatatctgc acctaaagct tttaagtgtt
    19681 ctacaatatc tgtataacct ctataaatat gttttacatt gtaaattgta gttacaccct
    19741 cagcaattaa accagcaata attaaacaag ctcctgctct taaatcgcta gcataaactt
    19801 cagcaccatg taatgttgat ggttttatcg ttgctgtgcc ttcgtcaact tcaatatttg
    19861 cacccatgcg ctttaattct tcaacatgtt taaaacgctc cggataaatc gtatcagtta
    19921 caaatgaagg accatttgcc ataaataata atggtgtaat aggctgttgc aaatcagtag
    19981 caaaacctgg atatactagt gttttaatat caacaaattg atatggcgca ttattattga
    20041 tgcgaattct ttcgtctctt acatcaacat tcacacctaa ttcactaaat ttagcagtta
    20101 atgtttctac atgtttcgga acaatattat ttaatataac attttctcca catgctgcag
    20161 cgatacacat atatgtgcct gcttcaattc tatcaggtat aacttgatac tcagaaccat
    20221 gtaattcttt gacaccattg attttaattg ttgatgtgcc cgctccctta atattagctc
    20281 ccatacttgt taagaagtta gcaacatcaa ctacttccgg ttctttagca gcattttcaa
    20341 ttacagtttg tcctgttgca taaactgcag ctagcataat gttaattgtt gcacctacgc
    20401 taaccatatc tagaaaaata tgcgcacctt ttaattcttt agcttcaatt ttcattgatg
    20461 tcgtgcttga ttcatcaatt tcagcaccta aagctttaaa tcctttaata tgttgatcaa
    20521 ttggacgtgg cccaagtgga catcctcccg gtaaaccaat cacacatttt ttaaatctac
    20581 ctaacatggc acccatcata tagtatgaag cacgtaatga ttcaacttta ttatttggta
    20641 atgcagcatt ttgtatttca gttgtatcaa cttctaattc cgtaccattt agtgatgcct
    20701 taatatttaa atcttctaat aaactcacta aagttttaac atcagagatt tgcggtaacc
    20761 cttctaattt cacatgtcct tgagctaata atgttgcagg aatgatagct acagcactgt
    20821 ttttcgcgcc actgatatta acttccccat ttagtgtgcg tccacctctt atttttatta
    20881 cctcttgagc catatcattt atctcctttg tccattactt tttaattttt tgcattatta
    20941 gcattataaa ttaattttac tttatttgta aaatagttta ttttaatcgt taaaatcttg
    21001 tctttctatt ttcgattgat taataacaaa tcattcacct ttactaatat atgctgttct
    21061 tccccacttg accatggtct aaagtctgat tattcaacta aatattgacg ttgttttgat
    21121 aattatttag ttttaattct acgatttgta cgtaaacttt tttcataaat tgctcatttt
    21181 ccttataatt atatcacaat caattttggt atattttacc atttttacgc tttcatttta
    21241 aaatcaaact gttttattta ttacttatac gacataaaaa agaagcaaac accattttgt
    21301 ctgcttcttt taaaattaat atccctacgt tattaataac ttaaaaacta aatattaatt
    21361 atttagcgcg gtttgaagta ccgaactctt taattttacc tttaactgtt tctttgatgg
    21421 cttcacgtgc aggtcctaag tatttacgag gatcgtaaac ttctttgtcg ttatttaaaa
    21481 cgtcacgaac tgcttttgct gaagcgattt ggttttcagt gtttacgtta attttagctg
    21541 taccaaatgg aattgctttt tggatatctt tagtcgggat accagtacca ccgtgtaata
    21601 ctaatggtaa acctgtagat aaaccgattt cttccatttc tttaaatcct aattttggtt
    21661 cacctttgta tggaccatga actgaaccta atgctggcgc taatgcatca ataccagttt
    21721 tttcaactag ttcttgacat tctttaggat cagcataaat gatgccgtct gctacaacat
    21781 catcttcttg tccaccaaca gtacctaatt cagcttctac agaaacacct ttttcatgag
    21841 cgtattcaac aactttttta gttgttgcta cgttttcttc gaatgggctg tgtgaagcat
    21901 cgatcattac tgatgtgaaa ccagcatcga tagcttcttt acatttttca aagcttgaac
    21961 catggtctaa atggattgct acaggaatag tgatgtttaa gtcatgcatt aacccttcaa
    22021 ccattttaac aattgtgtag aaaccgctca tgtaacgagc agcaccttca gaaacaccta
    22081 aaattacagg tgcattttct tcttgtgacg cttctaaaat tgcttgagtg aattctaggt
    22141 tattaatatt gtattgacct accgcataac cattttcttt tgcatcaatt aacatttctt
    22201 tcattgaaac taaaggcatg aaagttcctc cttgagtgct tacgcacgta tttttcaaag
    22261 taattatatc aaaaaaatag cgtcaatgca ttgaaatact gcagattaaa tgtgtatttt
    22321 ttgaaaaatg cgatagttat actaaatgca aacgtataca tttttataca atttaagccg
    22381 ttgcataata tgtagtcatt aattacaata ttttataagt agattggagg ataaagatgt
    22441 caaaaatctt aaacacacaa ctaataggta tctttaatag gctagaaaaa caatctttag
    22501 aaattcaaat ggccgcccaa tgtttaattc aagcaattgg tggcgaaggt tatgtttatg
    22561 taaaaggtta tgatgattta cagtttttcg aaagtttcat tctacatagt gatgagcgtt
    22621 taaaatctag ccgaaaactt gacgctatta aagactttaa agaaattgac tcaactgatc
    22681 gtgtattatt attcgcacca ttttataatg atcaagttgc attagatatt caaaaactta
    22741 tcgacttaga tatcgatgtg gtcttaataa gcaacaaacc taaaacagat gatttccctg
    22801 atcatctcgt acattttatc gatttatcta caccaagacc tatagtctat actgaagatt
    22861 atgataagat tgtccagcca catgcgatag cattgaatta tgtatactat gacatttata
    22921 cacaaatgat tgaaatgaca agagacttag aattataaaa gtcactattg ttatttcata
    22981 ccttgtcact aacataatat taaaattata cgaaaaaccg gtactttcaa tgtttaacaa
    23041 tgaaagtacc ggttttatta gcaaatttat ttattttgtt gatattttaa tgaagcttca
    23101 ataaatgatt taaaaatcgg gtgcggacga tttggtctag ataagaattc tgggtggaat
    23161 tgacaagcaa taaagaaatc atttgtcgga atctctacca tttctactaa acgtccatct
    23221 ggacttgtac cagaaattac cataccattt gcttctaatt gttctctata gtcattatta
    23281 aattcataac gatgacgatg tctttcttca atttccgctt taccataaac atcttgtgcc
    23341 aatgtgcctt ctttaataga acatggatat aagcctaagc gtaatgtacc acctaaatct
    23401 tcgatatctt tttgttctgg taataaatct ataattgggt atggtgttgc tgggtctaat
    23461 tcagctgaat gtgcgccttc aagtcctaat acgtttcttg aaaattcaac tgttgcaagt
    23521 tgcattccta aacaaatacc aaagaatggt acattgtttt ctctagcata cttaattgca
    23581 ctaattttac cttcacttgc acggaaacca aatccacctg gtactaaaat accgtcgaca
    23641 tctgcaaggt attcggctgc attttcatct gttacttcac ttgaatcaat ccatctaatg
    23701 tcaatatctt tggcaaaagg atatccagca tgtttcaatg attcaacaac tgataaatat
    23761 gcatcttgta agctaacata tttacctact aaaccaattg taattttacc atctaaatta
    23821 ttaacgatat ctaacaactg tttccattca tcaagctgtg tttcatattt cgcgtttaat
    23881 tgtaaacgtt taataacgat atcatccata ttttgttggc ttaattgtaa tggaatttcg
    23941 tataaagagt ctgcatcacg acattcaata acactttctt tattaatgtc acagaataat
    24001 gcaattttat cttttaaatc ttgtgtcatt tcatattcag ttcttacaac gattaagtct
    24061 ggttgaatac ctaagcctcg taattcttta acactatgtt gtgttggctt cgttttcatt
    24121 tctccagcag ctttaatata aggcagtaat gtacagtgaa catacataac attttctcta
    24181 cctaaatcgc tacgaatttg acgaatcgct tcaataaacg gtaatgactc aatatcacct
    24241 gttgtaccgc caatttcagt gataacaacg tctgcattcg tactttcccc tgcaagtaat
    24301 aaacgttctt taatttcatt tgtaatatgc ggaataactt gaactgttcc gcctaagtaa
    24361 tcaccacgac gttctttttt caatacgtgt gaatacactt taccggctgt cacatttgaa
    24421 aacttgttta aattaatatc aataaatctt tcgtaatgtc ctaagtctag gtcagtttct
    24481 gcaccatcat ccgttacgaa tacttcacca tgttgataag gactcattgt acctgggtca
    24541 acatttaagt atggatcgaa tttttgaatt gttacattta gacctctatc ttttaataat
    24601 ctacctagag aagatgctgt aatacccttc cctaatgatg aaactacgcc acctgttaca
    24661 aaaataaatt ttgtcatgtc ttgacctcct aattattaaa gggaatgctt cctcatcaat
    24721 tgtaaaaatt acgtaaaaca taacattaaa gcaagctcat tcaagttgtt tatttgtcga
    24781 attcgacaaa attaaattct gtttacttac cgtttccata ctgctatgtc actatgtata
    24841 tcatttacaa ctttgatttt tgcgttatca tttcttgatt tactcaatta aataaaaaaa
    24901 cgctccctct cacaaattct gtaaggggag cgtataaatt ttatacacgt gtcctattta
    24961 aaggagcccg aataaaattt tatcatcttt caactaaaag tcaaacaaaa aattaatcgt
    25021 tgaagtcttc ttcgtcttca aacacttctt cgtcttcgtc tagttcatct tcatcttctt
    25081 caatgactat atccgaatga ttgatttctt cttcaacctg ctcatcttct ggatcattta
    25141 gttcttcttg atcatctgtt tgagctggaa tatcatcgtc gtcatccatt tcatcttcgc
    25201 ccaataattt taagttttga tcttcttcat catctgcatc cagaatatcg aatttttgaa
    25261 tagttggtgc gattttctct tcaatatcat ctaccgaata ccaatcacgt aatccccata
    25321 aattttctcc aacatttaaa aaacgaccat ctgtgtttaa atctgtgtaa aattgtacaa
    25381 cgcgattttc aatttcttcg tactcataat cacctaacgc tctaaactca tcgatgatat
    25441 catataagtt catagtttcg cctttatcat tcaataatgt ataagccata tcaataaatg
    25501 atttttcatc aaccatttgt tttgtataat cttgaatttt cattgcccaa tacttccttt
    25561 cgaaggatat aatctaaatt ta
//
LOCUS       DAHHSC010000018        24960 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.018, whole genome shotgun sequence.
ACCESSION   DAHHSC010000018 DAHHSC010000000
VERSION     DAHHSC010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 24960)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24960)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24960
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.018"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            <1..333
                     /locus_tag="NBI57_000582"
     CDS             <1..333
                     /locus_tag="NBI57_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCD1821558.1"
                     /translation="FNTQSLSQSVAENIIRVYTVMTIILFVVIHAIILMGIVQYYLKR
                     GQKITKDKVKTADKDKKVVTEPKNTKVENGKVTTSVETKTEKAQDSLNDNTTKTMTSD
                     KPEDNQPK"
     gene            complement(766..870)
                     /locus_tag="NBI57_000583"
     CDS             complement(766..870)
                     /locus_tag="NBI57_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1821559.1"
                     /translation="MLLLERTSMSDFEMLMVVLTIIGLVLISTQDHKK"
     gene            1550..1708
                     /locus_tag="NBI57_000584"
     CDS             1550..1708
                     /locus_tag="NBI57_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001792784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HCD1821560.1"
                     /translation="MSKSSDALFNSLTQSFELIEGVYNELVTDNLKIVMYKPRKESTS
                     GRINRRFK"
     gene            complement(2366..3223)
                     /locus_tag="NBI57_000585"
     CDS             complement(2366..3223)
                     /locus_tag="NBI57_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HCD1821561.1"
                     /translation="MDNVKAIFLDMDGTILHENNQASTYTKDVINQLREKGYKVFLAT
                     GRSHSEIHQLVPQDFAVNGIISSNGTIGEVDGEIIFKHGLSLAQVQQITNLAKRQQIY
                     YEVFPFEGNRVSLKEDETWMRDMIRSQDPINGVSHSEWSSRQDALAGKIDWVTKFPEG
                     EYSKIYLFSSNLEKITAFRDELKQNHVQLQISVSNSSRFNAETMAYQTDKGTGIKEMI
                     AHFGIHQEETLVIGDSDNDRAMFEFGHYTVAMKNARPEIQALTSDVTAYTNEEDGAAK
                     YLAKHFLAD"
     gene            complement(3291..4073)
                     /locus_tag="NBI57_000586"
     CDS             complement(3291..4073)
                     /locus_tag="NBI57_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCD1821562.1"
                     /translation="MLIQLDQIGRMKQGKTILKNISWQIAKGDKWILYGLNGAGKTTL
                     LNILNAYEPATTGGVNLFGKMPGKVGYSAETVRQHIGFVSHSLLEKFQEGERVIDVVI
                     SGAFKSIGVYQDIDDEVRNEAHHLLKLVGISAKAQQYIGYLSTGEKQRVMIARALMGQ
                     PQVLILDEPAAGLDFIARESLLNILDSLSDSYPTLAMIYVTHFIEEITGNFTKILLLK
                     DGESVQQGLIDDILTSENMSRFFQKNVAVQRWNNRFSMAMLE"
     gene            complement(4305..6110)
                     /gene="glmS"
                     /locus_tag="NBI57_000587"
     CDS             complement(4305..6110)
                     /gene="glmS"
                     /locus_tag="NBI57_000587"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HCD1821563.1"
                     /translation="MCGIVGYIGYDNAKELLLKGLEKLEYRGYDSAGIAVVNDDNTTV
                     FKEKGRIAELRKVADSSDFDGPVGIGHTRWATHGVPNHENSHPHQSSNGRFTLVHNGV
                     IENYEELKGEYLQGVSFISETDTEVIVQLVEYFSNQGLSTEEAFTKVVSLLHGSYALG
                     LLDAEDKDTIYVAKNKSPLLLGVGEGFNVIASDALAMLQVTSEYKEIHDHEIVIVKKD
                     EVIIKDADGNVVERDSYIAEIDASDAEKGVYAHYMLKEIHEQPAVMRRIIQEYQDAEG
                     NLKIDQDIINDVKEADRIYVIAAGTSYHAGLVGKEFLEKWAGVPTEVHVASEFVYNMP
                     LLSEKPLFVYISQSGETADSRAVLVETNKLGHKSLTITNVAGSTLSREADHTLLLHAG
                     PEIAVASTKAYTAQIAVLSILSQIVAKEHGREADIDLLRELAKVTTAIEAIVDDAPIM
                     EQIATDFLETTRNAFFIGRTIDYNVSLEGALKLKEISYIQAEGFAGGELKHGTIALIE
                     DGTPVVALATQENVNLSIRGNVKEVVARGAHPCIISMEGLEKEGDTYVIPHVHELLTP
                     LVSVVALQLISYYAALHRDLDVDKPRNLAKSVTVE"
     gene            6791..8329
                     /locus_tag="NBI57_000588"
     CDS             6791..8329
                     /locus_tag="NBI57_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICB"
                     /protein_id="HCD1821564.1"
                     /translation="MSQTEEKKGIGRRVQAFGSFLSSMIMPNIGAFIAWGFIAAIFID
                     NGWLPNKDLATLAGPMITYLIPLLIAFSGGRLIYDLRGGIIAATATMGVIVALPDTPM
                     LLGAMIMGPLVGWLMKKTDQLIQPRTPQGFEMLFNNFSAGILGFIMTIAGFKILAPLM
                     KFIMHILSVAVEALVHAHLLPLVSILVEPAKIVFLNNAINHGVFTPLGADQAAKAGQS
                     ILYTIESNPGPGLGILLAYMIFGKGTAKATSYGAGIIHFLGGIHEIYFPYVLMRPLLF
                     IAVILGGMTGVATYQATGFGFKSPASPGSFIVYCLNAPRGEFLHMLLGVFLAALVSFV
                     VAALIMKFTREPKQDLEAATAQMENTKGKKSSVASKLVSSDKNVNTEENASGNVSETS
                     SSDDDPEALLDNYNTEDVDAHNYNNINHVIFACDAGMGSSAMGASMLRNKFKKAGIND
                     ITVTNTAINQLPKDAQLVITQKKLTDRAIKQTPNAIHISVDNFLNSPRYEELLNNLKK
                     DDQA"
     gene            8364..10496
                     /locus_tag="NBI57_000589"
     CDS             8364..10496
                     /locus_tag="NBI57_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HCD1821565.1"
                     /translation="MLLSTREKEMIALLIKYHSQYITIHDIAQQLAVSSRTIHRELKG
                     VEAYLTSFSLTLERANKKGLRIAGADSDLNDLKQSIAQHQTIDLSVEEQKVIIIYALI
                     QAKEPVKQYSLAQEIGVSVQTLAKMLDDLELDLNKYQLSLSRKRGEGIYLVGTESKKR
                     EFLSQLMVNNLNSTSVYSVIENHFVFHSLNQIHKDFVDLERIFNVERLLMDYLSALPY
                     QLTESSYLTLTVHIVLSISRIKNGEYVALNDDIYDSVQNTFEHKVASELADKLGQIYD
                     VTFNQAEIAFITIHLRGAKRKNLNDTSLNNRCEENKIKAFVNKVEMISGMTFADLDTL
                     VDGLTLHLNPAINRLQANIETYNPLTDMIKFKYPRLFENVRLALNDCWPDLIFPESEI
                     AFIVLHFGGSIKNQGNRFLNILVVCSSGMGTSRLLSTRLEQVFSEIERITQASVSDLK
                     SLDLSQYDGIISTVNLDIDSPYLTVNPLLPDSDISYVAQFLNTKSTFQETHDKSSNMI
                     DKDDVHVETKDVDGNTSFENEQTSYLTSVFEKHLSDEKSEQLLHHMRSGLTLLDSVKI
                     VSTEVKQWQTYIADYLYQCDVINDPTSFAELLEQRLIDNPGWILSPYPVAIPHLRDNM
                     IKHPMILITVLEEPLTLPSIQNDNQTIKYMISMFIPDNDFMASLVSDLSEFLSLKLES
                     IDTFMENPQELETLLRNKFLERIKKQFI"
     gene            10508..10942
                     /locus_tag="NBI57_000590"
     CDS             10508..10942
                     /locus_tag="NBI57_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HCD1821566.1"
                     /translation="MSELFSNDNIFLNVNVNSQNEAIEKAGKALVDSGAVTDAYIQAM
                     KDREQVVSTFMGNGLAIPHGTDEAKTNVIHSGLTLLQIPEGVDWDGEVVKVVVGIAGK
                     DGEHLDLLSKIAITFSEEENVDRIVQAKSAEEIKQVFEEADA"
     gene            10942..12048
                     /locus_tag="NBI57_000591"
     CDS             10942..12048
                     /locus_tag="NBI57_000591"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HCD1821567.1"
                     /translation="MKAVHFGAGNIGRGFIGYILADNNVKVTFADVNEEIINALAHDH
                     QYDVILADESKTTTRVNNVDAINSMQPSEALKQAILEADIITTAVGVNILPIIAKSFA
                     PFLKEKTNHVNIVACENAIMATDTLKKAVLDITGPLGNNIHFANSAVDRIVPLQKNEN
                     ILDVMVEPFYEWVVEKDAWYGPELNHIKYVDDLTPYIERKLLTVNTGHAYLAYAGKFA
                     GKATVLDAVKDSSIEAGLRRVLAETSQYITNEFDFTEAEQAGYVEKIIDRFNNSYLSD
                     EVTRVGRGTLRKIGPKDRIIKPLTYLYNKDLERTGLLNTAALLLKYDDTADQETVEKN
                     NYIKEHGLKAFLSEYAKVDDGLADEIIEAYNSLS"
     gene            complement(12367..19812)
                     /locus_tag="NBI57_000592"
                     /pseudo
     CDS             complement(12367..19812)
                     /locus_tag="NBI57_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500880.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1542 domain-containing protein"
     gene            complement(20089..21444)
                     /locus_tag="NBI57_000593"
     CDS             complement(20089..21444)
                     /locus_tag="NBI57_000593"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HCD1821568.1"
                     /translation="MGKYFGTDGVRGVANQELTPELAFKLGRYGGYVLAHNKGEKHPR
                     VLVGRDTRVSGEMLESALIAGLISIGAEVMRLGIISTPGVAYLTRDMGAELGVMISAS
                     HNPVADNGIKFFGSDGFKLSDEQENEIEALLDQENPELPRPVGNDIVHYSDYFEGAQK
                     YLSYLKSTVDVNFEGLKIVLDGANGSTSSLAPFLFGDLEADTETIGCSPDGYNINEKC
                     GSTHPEKLAEKVVETESDFGLAFDGDGDRIIAVDENGQIVDGDQIMFIIGQEMHKNQE
                     LNNDMIVSTVMSNLGFYKALEQEGIKSNKTKVGDRYVVEEMRRGNYNLGGEQSGHIVM
                     MDYNTTGDGLLTGIQLASVIKMTGKSLSELAGQMKKYPQSLINVRVTDKYRVEENVDV
                     KEVMTKVEVEMNGEGRILVRPSGTEPLVRVMVEAATDEDAERFAQQIADVVQDKMGLD
                     K"
     gene            complement(21471..22403)
                     /locus_tag="NBI57_000594"
     CDS             complement(21471..22403)
                     /locus_tag="NBI57_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbbR-like domain-containing protein"
                     /protein_id="HCD1821569.1"
                     /translation="MLESKWGLRFIAFLLALFFFLSVNNVFGNIFNTGNLGQKSSKTI
                     QDVPVEILYNTKDLHLTKAPETVNVTISGPQSKIIKIENPEDLRVVIDLSNAKAGKYQ
                     EKYQVKGLADDIHYSVKPKLANITLENKVTKKMTVQPDVSQSDIDPLYKITKQEVSPQ
                     TVKVTGGEEQLNDIAYLKATFKTNKKINGDTKDVAEVTAFDKKLNKLNVSIQPNEVNL
                     QVKVEPFSKKVKVNVKQKGSLADDKELSSIDLEDKEIEIFGSRDDLQNISEVDAEVDL
                     DGISESTEKTVKINLPEHVTKAQPSETKAYINVK"
     gene            complement(22405..23214)
                     /locus_tag="NBI57_000595"
     CDS             complement(22405..23214)
                     /locus_tag="NBI57_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00159 family protein"
                     /protein_id="HCD1821570.1"
                     /translation="MDFSNFFQNLSTLKIVTSILDLLIVWYVLYLLITVFKGTKAIQL
                     LKGILVIVIGQQISMILNLTATSKLFDIVIQWGVLALIVIFQPEIRRALEQLGRGSFL
                     KRYTSNTYSKDEEKLIQSVSKAVQYMAKRRIGALIVFEKETGLQDYIETGIAMDSNIS
                     QELLINVFIPNTPLHDGAMIIQGTKIAAAASYLPLSDSPKISKSLGTRHRAAVGISEV
                     SDAFTVIVSEETGDISVTFDGKLRRDISNEIFEELLAEHWFGTRFQKKGVK"
     gene            complement(23403..24311)
                     /gene="rocF"
                     /locus_tag="NBI57_000596"
     CDS             complement(23403..24311)
                     /gene="rocF"
                     /locus_tag="NBI57_000596"
                     /EC_number="3.5.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginase"
                     /protein_id="HCD1821571.1"
                     /translation="MTKTKAIDIIGAPSTFGQRKLGVDLGPTAIRYAGLISRLKQLDL
                     DVYDKGDIKVPAVNIEKFHSEQKGLRNYDEIIDVNQKLNKEVSASIENNRFPLVLGGD
                     HSIAVGSVSAISKHYNNLGVIWYDAHGDLNIPEESPSGNIHGMPLRILTGEGPKELLE
                     LNSNVIKPENIVLIGMRDLDKGERQFIKDHNIKTFTMSDIDKLGIKEVIENTIEYLKS
                     RNVDGVHLSLDVDALDPLETPGTGTRVLGGLSYRESHFALELLHQSHLISSMDLVEVN
                     PLIDSNNHTAEQAVSLVGTFFGETLL"
     gene            complement(24449..24521)
                     /locus_tag="NBI57_000597"
     tRNA            complement(24449..24521)
                     /locus_tag="NBI57_000597"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(24486..24488),aa:Lys,seq:ttt)
     gene            complement(24525..24599)
                     /locus_tag="NBI57_000598"
     tRNA            complement(24525..24599)
                     /locus_tag="NBI57_000598"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(24565..24567),aa:Gln,seq:ttg)
     gene            complement(24609..24692)
                     /locus_tag="NBI57_000599"
     tRNA            complement(24609..24692)
                     /locus_tag="NBI57_000599"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(24656..24658),aa:Tyr,seq:gta)
     gene            complement(24722..24797)
                     /locus_tag="NBI57_000600"
     tRNA            complement(24722..24797)
                     /locus_tag="NBI57_000600"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(24762..24764),aa:Val,seq:tac)
     gene            complement(24805..24876)
                     /locus_tag="NBI57_000601"
     tRNA            complement(24805..24876)
                     /locus_tag="NBI57_000601"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(24841..24843),aa:Glu,seq:ttc)
     gene            complement(24878..24952)
                     /locus_tag="NBI57_000602"
     tRNA            complement(24878..24952)
                     /locus_tag="NBI57_000602"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(24918..24920),aa:Asn,seq:gtt)
BASE COUNT         7378 a         4473 c         3966 g         9143 t
ORIGIN      
        1 tttaatacgc aatcactctc tcaaagtgtt gcagaaaata tcataagagt ttatacagta
       61 atgacaatca ttttattcgt tgttattcat gcaatcattt taatgggtat cgttcaatat
      121 tacttaaaac gtggtcaaaa aattactaaa gataaagtaa aaacagctga caaggataaa
      181 aaagttgtca ctgaaccaaa aaatacaaaa gtagaaaatg gtaaagtgac aacatctgtt
      241 gaaacaaaaa cagaaaaagc acaagattca ttaaatgata atacaacaaa aacgatgact
      301 tcagataaac cagaagacaa tcaaccaaaa taaattttgg agccccttac aactttagtt
      361 gttaggggct cttatgcatg cgcttagcgc aggcgctcct acttttcaac tttagttgtt
      421 aggggcttct ttctaaaata actaagtgaa ttatctatat atgaagaggt ctccaatact
      481 ttttatataa ttcgtgatta cattctatac ttttaattgt attttctgct gtatggaaag
      541 ttggttttat taattctgat tatgttcaga actacaattc ataagaaaaa tgtgttaaaa
      601 tatatttgta gtaagtagaa gcaaaagatg aaaatcttta actcttgaaa cacaaaaagg
      661 gcaacactcg gaaacatgtt accctaatga gcccgttaaa aagacggtga ccttatattt
      721 tatttaaaaa tagccttcaa aatgccggtc aaagcgaata gaaggttatt ttttatggtc
      781 ttgagtacta atcaatacta aaccaatgat tgttaataca accatcagca tttcaaaatc
      841 agacatgcta gtcctttcta ggagtaacaa ctataaccat aggcatcacc cctttattaa
      901 gagattagcc accatctatc caacttgctc aataaatatt ataacatgca cacttttaaa
      961 cagtaattca tagtataagt tttttcattt cttaatcaac tcgataaaca gttgagtgac
     1021 atacccttgt ctaactagct atttcttccc ctgttatcgt ttgttcatca tataaaaatt
     1081 taatttctca ttttttattc tctgataaag aagagcacca accttttcgg ctgatatgct
     1141 cccttcaaaa tagacattga aaaatgaaaa ctttgaaaga gaaatcgtca gtctattatt
     1201 gataagtatt atcctatcct cgaaaaatta ctttcagaca ctagtgaaca aaaattttac
     1261 tataagcgta tactttggca atattcgaag gaaaaacatg gattaacctg tgcatattat
     1321 acatttagag cttatatact taaacatgat gaatttactc cttatattat gaaatgttat
     1381 caatagatgt cgcctaaaga tacaacaaga ttcgagacaa aacctgatca tcaagcacag
     1441 tttgactgga aagaaagcat taactttaaa acgaaagata atcaaaggat tccactatat
     1501 ataggtgtgc tactacttta ttattctcgt tttataatca tgcataacaa tgagtaaatc
     1561 aagtgatgcc ttatttaatt cattaacaca atcgtttgag ctaatagaag gtgtatataa
     1621 tgaacttgta acggataact tgaaaatagt catgtataag ccaagaaaag aaagtacaag
     1681 tggtcgaata aatcgtagat ttaaataatt cgctgatggt tttaaatttc aagtaaaacc
     1741 ttgtatttcc ggcctcccta gaaaaagata aagttgaatc aataacaaaa atattagatg
     1801 aaattaatgc ttaccaagac gaattgcatt tggaagagat tcctaagttt ttttaatctt
     1861 aatgaacgat ttaactacta agttcatact agtacaggta aattgcctat tactgcctaa
     1921 gagaaagaaa aaattttctt acaaccacta cagaatgtga atgtaaaaaa ctcatataaa
     1981 gtgaaacata tcttacttat ggaatgaaat gtttgattat cccaaaatca caaatgcgat
     2041 tttggagttg ttaggggctc taacgcaaat acacccctac ttaaaataac ggtagttgtt
     2101 atagtatact taaatgcata aaccatataa aaggtgatgc taatatgaca tacggttcaa
     2161 ttgtagctat actctgtgcc ctatttgtga ttttcttcat tccatatatg gagaagaaag
     2221 acaaaaagaa gaagtaacac tagtatctgg attactagca tacaaaggag cagtaagaca
     2281 ttttctaata gaaaataact ttactgctct ttgtacatta ctcatcaact attgtaaatt
     2341 aaataataaa taactaccta ttttattaat cagctaaaaa atgctttgct aaatattttg
     2401 ctgcgccatc ctcttcattc gtgtatgccg ttacatctga agttaatgct tggatttcag
     2461 ggcgtgcatt tttcatagca actgtataat gaccaaattc aaacattgct ctatcattgt
     2521 cgctatctcc aataactaac gtttcttctt gatgaatacc aaaatgtgca atcatttctt
     2581 taatgcctgt acctttatca gtttgataag ccattgtttc cgcattaaat cttgatgaat
     2641 ttgaaacact aatctgtagt tgcacatgat tttgctttaa ttcatctcta aatgctgtta
     2701 ttttttctaa attagaactg aatagataaa tttttgaata ttcaccttca ggaaacttag
     2761 ttacccaatc tatcttacca gcaagcgcat cttgtcttga agaccattca ctatgactta
     2821 cgccattaat aggatcttga ctacgaatca tatctcgcat ccatgtttca tcttctttta
     2881 aagaaactct attaccttca aaaggaaata cctcataata aatttgttgg cgcttagcta
     2941 aattagtaat ttgttgcact tgagccaatg ataaaccatg cttgaagata atttctccat
     3001 ctacttctcc aattgttcca tttgaactaa tgatgccatt aaccgcaaaa tcttgaggta
     3061 caagttgatg tatttcagaa tgcgaacgtc ctgttgccaa aaatacttta tatcctttct
     3121 ctctcaattg attaatgaca tctttcgtat acgttgatgc ttgattattt tcatgtaaaa
     3181 ttgttccatc catgtccaaa aatattgctt ttacgttatc catattgccc tccactgtcc
     3241 attttatctt aattaaattt tgtcattatt acttattatt tgcaaaatat ttactctaac
     3301 attgccatag aaaatcgatt attccatctt tgaactgcta catttttctg gaaaaatcgt
     3361 gacatgtttt cagatgtcaa aatatcatct attaatcctt gttgtacact ttccccatct
     3421 tttaatagca aaattttagt aaagttacca gtaatttctt caataaagtg cgtcacataa
     3481 atcatcgcaa gcgttggata tgaatctgac aatgagtcaa gtatattcaa tagtgactca
     3541 cgagcaataa agtctaaacc agctgctggc tcatctaaaa ttaaaacctg aggttgcccc
     3601 attaaagctc gtgcaatcat cactcgttgt ttttcacctg tagataaata accaatatat
     3661 tgttgcgctt tagcagatat tccaactaat ttaagtaaat gatgtgcttc attacgtacc
     3721 tcatcatcaa tatcttgata aacaccaatt gatttaaaag caccgcttat caccacatcg
     3781 attactcttt caccctcttg aaacttttcc agtaaactat gagatacaaa acctatatgt
     3841 tgtcgtacag tctctgcaga ataccctacc ttgcctggca ttttaccaaa tagattaaca
     3901 ccgccagttg tcgcaggctc atatgcattt aatatattta atagcgttgt cttaccagca
     3961 ccattcaacc catataatat ccatttatca cctttagcaa tttgccaaga aatatttttt
     4021 aaaattgttt ttccttgctt cattcgccca atttgatcta attgaattaa catgcgaggt
     4081 caccccttta ttgctactcc atacttctca taaaatcata ttaacataac acccttaatt
     4141 gtcagactat ttaaataaat aaaacacttc ctttttacgc atttctgcca aattaagatg
     4201 aagtaaaagc taagtcgacc taaaaaagca cccttctagt cgattaatct aaaagggtgc
     4261 catatacttt aattttaata catgattgat tctaaaaaag tgaattattc tacagtaact
     4321 gatttagcaa ggttacgtgg tttatccaca tctaaatctc tgtgtaatgc tgcataatat
     4381 gaaatcaatt gtaatgcaac tactgaaact aatggtgtca ataattcatg tacatgagga
     4441 atgacataag tgtcgccttc tttttcaaga ccctccatag aaataataca tggatgtgca
     4501 ccacgtgcta ctacttcttt aacgttacca cgaattgata aattaacgtt ttcttgagtt
     4561 gctaaagcaa caactggtgt gccatcttcg attaatgcaa tcgtaccatg tttaagttcg
     4621 ccaccagcaa aaccttctgc ttgaatgtaa gaaatttctt taagttttaa cgcaccttct
     4681 aaacttacgt tatagtcaat agtacgtccg ataaagaatg cattgcgtgt tgtttctaag
     4741 aaatctgttg caatttgttc cataattggt gcatcgtcaa caattgcttc tattgctgtt
     4801 gttacttttg ctaattctct caataaatca atatctgctt cacgaccgtg ctcttttgca
     4861 acgatttgag acaagattga taatactgca atttgtgcag tatatgcttt tgtagatgca
     4921 actgcgattt caggacccgc atgtaataac aatgtgtggt ctgcttcacg tgataaagtt
     4981 gaacctgcaa cattagtgat tgttaatgat ttatgaccta atttattagt ttcaactaat
     5041 actgcacggc tatctgctgt ttcacctgat tgagaaatat aaacgaacaa tggtttttca
     5101 gataataatg gcatgttgta gacaaactct gatgcaacgt gtacttcagt tggtacgcca
     5161 gcccattttt ctaaaaattc tttacctact aaacctgcat ggtagcttgt acctgctgca
     5221 ataacgtaaa tgcggtctgc ttctttaaca tcattgatga tgtcttgatc aattttcaag
     5281 ttaccttctg catcttgata ttcttgaata atacgacgca ttactgctgg ttgttcatga
     5341 atttctttta acatgtagtg tgcataaaca cctttttcag catctgatgc atcaatttca
     5401 gcaatatatg aatcacgttc tacaacgttt ccatctgcat ctttaataat aacttcatct
     5461 tttttaacaa taacgatttc atggtcatgg atttctttat attcgcttgt cacttgtaac
     5521 attgcaagtg cgtctgatgc gataacattg aaaccttcac caacacctaa taataatggt
     5581 gatttatttt tagcaacata gattgtgtct ttgtcttcag catctaataa acctaatgca
     5641 tatgaaccat gtaataatga cacaactttt gtaaatgctt cttcagttga aagtccttga
     5701 tttgaaaagt attcaactaa ttgaacgata acttctgtat ctgtttctga aatgaatgat
     5761 acaccttgta agtattcacc ttttaactct tcatagtttt caataacacc gttatgaact
     5821 agagtaaaac ggccatttga tgattgatgt ggatgagagt tttcatgatt cggtacaccg
     5881 tgtgttgccc aacgtgtgtg accgattcca acaggtccat caaaatcgct actatcagca
     5941 actttacgta attctgcaat acgacctttt tctttaaata cagttgtatt atcatcattt
     6001 actaccgcga tacctgcaga gtcataacct ctgtattcta atttttctaa accttttaat
     6061 aataattctt tggcattatc atagccaata taaccaacaa ttccacacat aacgacattt
     6121 tcctccatat tggaatagta cgcgtaaatt atgatttatt gccgataatt tagattgaca
     6181 atctgctttc ataatataaa taggaacatg ctattatcgc attcatccat aacaaattaa
     6241 gcatagttat ttttacaact atacaaattg ctcacactgt actttccata ttaatatttt
     6301 ttatattcaa tttctggcga tcttattaac tttgtccatt aagtcaccct aatgttttac
     6361 ttaataagct aacgaatgag ccacatccgg gatagcatcc gccgatctat tcgatcacta
     6421 tcctcctcgt ctacaaatac atatattgca ctctataaag gccactcata tattaacctt
     6481 taatcttcaa atacaaatat ttatttgcac aggcgcttta actgtactgc cgaactttcc
     6541 ccctttccat taatcattat tgtacaacgg tgttgttttg ttttgcaaat attttcacaa
     6601 taaaatttta aaaatcctaa aacaattttt ttgttttact ttttcaaaat atctatactg
     6661 tcacattgat gacactttat ttaattttgt cacatttatt ttgacaaagt tgatttttgt
     6721 ttatattgag taacaagtaa cgtctctata cactatatat agtcacatat attaaaaaag
     6781 aggtgtaaat atgtcgcaaa ctgaagagaa aaaaggaatt ggtcgtcgcg ttcaagcatt
     6841 cggatcattt ttgagtagta tgattatgcc aaacattggt gcattcatcg cttggggttt
     6901 catcgcggcc atttttatag ataatggatg gttacctaac aaagatttag ctactttagc
     6961 aggaccaatg attacttatt taatcccatt attgatcgca tttagtggtg gtcgtttaat
     7021 ttatgattta cgtggtggta tcatcgcagc aacagcaact atgggggtca tcgttgcatt
     7081 acctgataca ccaatgttac ttggtgcaat gattatgggt ccacttgttg gttggttaat
     7141 gaagaagacc gaccaattga ttcaacctag aacgccacaa ggttttgaaa tgttatttaa
     7201 taacttttct gctggtattt taggatttat catgacgatt gcaggattta aaattttagc
     7261 accacttatg aaatttatta tgcatatttt atcagtggca gttgaagcat tagtacatgc
     7321 acacctactt ccacttgtaa gtattttagt agagcctgca aaaattgtat ttttaaacaa
     7381 tgcgattaac catggtgtat tcacaccgct tggtgcagat caagctgcaa aagctggtca
     7441 atcaatttta tacacaattg aatctaaccc tggaccaggt ttaggtatct tacttgctta
     7501 tatgattttt ggaaaaggca ctgcaaaagc aacgtcatat ggtgctggaa ttatccactt
     7561 cttaggtggt attcatgaaa tttatttccc atatgtatta atgcgtcctt tattatttat
     7621 cgctgttatt ttaggtggaa tgactggtgt tgcaacttat caagcaacag gatttggatt
     7681 taaaagtccc gcgtcaccag gttcatttat agtttactgt ttaaatgcac ctagaggtga
     7741 attcttgcac atgttgctcg gtgtcttcct tgctgcactt gtatcattcg ttgtagctgc
     7801 tttaattatg aagttcacta gagaaccaaa gcaggattta gaagctgcga cagctcaaat
     7861 ggaaaatact aaagggaaaa aatcaagcgt tgcttctaag ttagtatctt ctgataaaaa
     7921 tgttaataca gaagaaaatg ctagtggtaa tgttagtgaa acatcttcat cagatgatga
     7981 tcctgaagcg ctattggata attacaacac tgaagatgtt gatgcacaca attacaataa
     8041 tataaatcat gttatttttg cctgcgatgc gggtatgggt tctagtgcaa tgggtgcaag
     8101 catgttacgt aataaattta aaaaggcggg cattaatgat attacagtta caaatactgc
     8161 gattaatcaa ttgccaaaag atgctcaatt agttatcact cagaaaaaac taactgatcg
     8221 tgctattaaa caaacaccaa atgccatcca tatttcagtg gataatttcc ttaattcacc
     8281 aagatatgaa gaacttttaa ataatctaaa aaaagatgat caagcataat aattaaataa
     8341 attaaaaaat ggaggatacc gccatgttat tgagtacacg tgaaaaagaa atgatagccc
     8401 tattgattaa gtaccacagt caatatatca ctatacacga cattgctcag caacttgcgg
     8461 tgtcctctcg tactattcac cgtgaattaa aaggtgttga agcatattta acttcatttt
     8521 cattaacttt agaacgcgca aacaaaaaag ggctacgcat tgctggcgca gattctgatt
     8581 taaacgattt gaagcaatcg attgcacaac atcaaaccat tgacttatct gttgaagagc
     8641 agaaagtaat tattatatac gctttgatac aagccaagga gccagttaaa caatatagtt
     8701 tagcgcaaga aattggcgtt tctgtccaga ctttagcaaa gatgttagat gatttagagc
     8761 ttgatttaaa taagtaccaa ctatctttat ctcgaaagcg tggcgaaggc atttacttgg
     8821 taggtactga atcaaagaaa cgtgaatttt taagtcaatt aatggtgaat aacttaaata
     8881 gtactagcgt ttattcagta attgaaaatc attttgtctt tcattcatta aatcaaatcc
     8941 acaaagactt tgttgactta gagcgcattt ttaatgttga aagactatta atggactacc
     9001 taagtgcctt accctaccaa cttaccgaat caagttattt aactttaact gtccatatcg
     9061 tgctatccat ttcacgtata aaaaatggag agtatgtcgc attaaacgat gatatttatg
     9121 attctgtaca aaacacattt gaacacaaag tagcaagcga acttgctgat aaacttggtc
     9181 aaatatatga cgtcacgttt aatcaggcag aaattgcttt cattactatc catttacgtg
     9241 gagctaaacg aaaaaatctt aatgatacat cattaaataa tcgttgtgaa gaaaacaaaa
     9301 ttaaagcgtt tgttaacaaa gtagaaatga tttccggtat gacatttgca gatttggata
     9361 ctttagtaga tggactgacg ctacacctta atcctgcaat caatcgtttg caagctaata
     9421 tcgagaccta taatccgtta acagacatga ttaagttcaa atatccaaga ctatttgaaa
     9481 atgtaagatt agctttaaat gattgttggc ctgatttgat ttttccagag agtgaaattg
     9541 cttttatagt tttacacttt ggtggctcga ttaaaaacca aggtaatcga tttttaaaca
     9601 tattagtcgt ttgcagcagt ggtatgggaa ctagtcgtct attatcaact cgtctagagc
     9661 aagtttttag tgagattgag cgtattacac aagcatcagt cagcgatttg aagtcactag
     9721 atttaagtca atatgatggc attatttcta ctgtgaattt agacatcgac tccccctatt
     9781 taacggtaaa cccattatta ccagatagtg atatcagtta tgtcgcacag tttttaaata
     9841 caaagtctac gttccaagag acgcatgata aatcatcaaa catgattgat aaggatgatg
     9901 ttcatgttga aacgaaagat gttgatggca acacatcttt tgaaaatgaa caaacttcat
     9961 acttaacttc agttttcgaa aaacatttaa gtgacgaaaa atcagaacaa ttattgcatc
    10021 atatgcgttc gggtttaact ttgcttgatt cagtgaaaat agttagtacc gaagttaaac
    10081 agtggcaaac atatatcgca gattatctat atcaatgcga tgtaataaac gatccaacgt
    10141 cattcgctga actactagag caacgattga ttgacaatcc aggatggata ttaagtccat
    10201 atcctgttgc aataccacac ctaagagaca atatgattaa acaccctatg attctaatca
    10261 cagttttaga agaaccgtta acattgccta gtattcaaaa tgacaatcaa acaattaaat
    10321 atatgatttc catgtttatt cctgacaatg attttatggc atcactggta agtgacttgt
    10381 ccgaattttt aagtttgaaa ttagaatcta ttgatacttt tatggaaaat ccacaggaac
    10441 ttgaaacatt attaagaaac aaatttttag aacgaattaa aaaacaattt atttaggagt
    10501 gtacaaaatg agcgaattat ttagtaatga caatatcttt ttaaatgtaa atgttaacag
    10561 ccaaaatgaa gcaattgaaa aagcaggtaa agccttagtt gatagtggtg ctgtaacaga
    10621 tgcttatatt caagcaatga aagatcgtga gcaagttgta tcaacattta tgggaaatgg
    10681 cttagcaatt cctcatggca cagatgaagc taaaacaaat gtgattcact caggtttaac
    10741 attattacaa atccctgaag gcgttgactg ggatggcgaa gtagttaaag ttgtagtggg
    10801 aatcgctggt aaagatggcg aacatttaga cttgttatct aaaattgcaa ttacatttag
    10861 cgaagaagaa aatgtggatc gtatcgttca agcaaaatct gcagaagaaa ttaaacaagt
    10921 attcgaggag gcagatgcat aatgaaagca gttcactttg gtgctggtaa cataggtcgt
    10981 ggtttcattg gttatattct tgcagacaac aatgttaaag taacatttgc agacgtcaat
    11041 gaagaaatca ttaatgcttt agctcatgat catcaatacg atgttatttt agctgatgag
    11101 tctaaaacaa cgacgcgcgt gaataatgtt gatgcaatta attcaatgca accttctgaa
    11161 gcgttgaaac aagcaattct agaagctgat attattacaa cagctgttgg tgttaacata
    11221 ctacctatta ttgctaaatc ttttgcgcct ttcttaaaag aaaaaacaaa ccatgttaat
    11281 attgttgctt gtgagaatgc tattatggca actgatacat tgaaaaaagc agtacttgat
    11341 attactggcc ctcttggtaa caatattcat tttgctaact cagcagttga tagaattgta
    11401 ccattacaaa agaatgaaaa tatattagac gttatggttg agccatttta cgaatgggtt
    11461 gttgaaaaag atgcatggta tggtccagaa ctaaaccata ttaaatatgt tgatgattta
    11521 acaccatata ttgagcgtaa attattaact gtgaatacag gacatgcata tttagcgtat
    11581 gctggtaaat ttgcaggtaa agctaccgta ttagacgctg taaaagatag ttcaattgaa
    11641 gctggcttac gccgtgtttt agctgaaaca agtcaatata ttactaatga atttgatttt
    11701 actgaagcgg aacaagctgg ttatgttgaa aaaataatag accgtttcaa caattcttat
    11761 ttatctgatg aagtgacacg tgtcggacga ggtacgttac gtaaaattgg ccctaaagat
    11821 agaattataa aaccattaac atatctttat aataaagatt tagaacgcac tggtttatta
    11881 aatacagctg cattgttatt gaagtatgat gatacagcag accaagaaac tgttgagaaa
    11941 aataattaca ttaaagaaca cggtttaaaa gcgtttttaa gcgaatatgc taaagttgac
    12001 gatggcttag ccgatgaaat aattgaagcg tacaattcac tttcataatt tattgagctt
    12061 tgtttgaaac aagaagtttc caacgttatt cgttaacaat cagtaataat gtagtagttc
    12121 cgttgaatta acaatattaa atttctgaac ataaaaaata ctcccttcaa catagacact
    12181 taacttgtgt tatgtatgaa aggagtattt ttgcgttaat aatttgtttt attttcgagc
    12241 cacagcgacc tattcaattg ctattggtca ttactaaaac aaattcatat taactgttag
    12301 acttggttac ttagtaagga atatttccct atgaaataac tagatgttca cattcttgaa
    12361 taaattttat tcttcagttt gttggtcttt cttagtgaat cttctaatta agaatgccat
    12421 acctgcacct agagctaatt cagcatatgg taaatcgtca ttatgtgaca taccagtatc
    12481 tggtaaagtt ttagcttgtt gtttaggctt attaactttt ccttgttggt ctgattttgc
    12541 atttgctttg ttttcttcag gcttagttac attaagcata tcttgatgca tactatgact
    12601 accatttgaa actgttgctg gagatgcatt ggcaccgtcg ttttgcgtag ctttattgtt
    12661 tgcagctgaa ccaactgatt tttgcgtatc attagtttgc atatctttag ctttattgtt
    12721 tgcaactggg ccaactgatt tttgcgtatc attagtatct gctgttgccg tatcatcttt
    12781 ttggctaaca ttagttgaag tcattttatc ttttgcttca gaagatgcag atgttgatgg
    12841 tttattcgaa acttcagtat cagctttgct tggcgattta tctgcttcgt tagatgcaac
    12901 gttagtttca gacttaagtt gtcctgcatc agtttgattt gtcgtacttt cttctttatc
    12961 ttttgatgta ttagaaggta catttggttc tgttatgtct gctgaaggca atgtttcagt
    13021 tgttgattca accatacttt gatttgttga atctctacca tctttttctg ccttagcttt
    13081 attttcagat tttggttgtg caaccttgcc attagttgat tgagattcag cactattatt
    13141 tacttcagca ttttgttttg aatcatttac agatgcatta tctttactat cagcagatga
    13201 tgctgcttct gtgctcgcag ttgttggagc cgttgctgtt gatcctgttg gtgcattctc
    13261 gtttgttgct gtagttgtac tattgttatt tgttgtgctt tctgctggcg ttgcattatc
    13321 tgtttctgtt acaggtttat cagttgtgcc gttattagtt gattctactt ctggtttact
    13381 agttacatcg ttatccattg tcggactgtt tgttgatgca tctgcactag aattgttatt
    13441 agcttgcggt ttatcctttg catcatcagt tgctgatgtt actgttgttt cacctgttgc
    13501 cgcatcacta ttatttggtg ttgtcggaga agcgtctgct ttgccattat ctgtcgtctc
    13561 agatacgtta ggttgtccag tgttttctgg tgttgcatta gcatttgaat ttgctgttgc
    13621 atcattatta tctgtaccat tattagtatc attagcatct ggatcattct gaggcacaat
    13681 cgcttcaatt gcaggtatcg ttacattttg taattcagca acctctgtat ttgtttgtgt
    13741 tttatctaat ttatcagcaa atctgtcaaa atatctacct aaatctgtac gtgcaatttt
    13801 tttcgccgat gcatctgcat ctgcattttt aattatttct atttgcttgt taaccacttc
    13861 tctaattgct tccaaagcat ttttcttaac tactggatta atacgttgtg ctttaagttg
    13921 ttcaagcgca ctatttttga cagtagcgat ttctgcattt gtagtttgat cagaaatatc
    13981 ttcagttgct tttgataaaa tgtcttctaa agcattcgta aacgcttctt tttcttcagt
    14041 tgtagcatca gcgtcgacat ttacacctgc ttcaatctgg tctagtgcag tttctaattc
    14101 ttcgatagct ttttgttttt ctgttgagtc aatttgaatg ttatcaaatg cctcaagtcc
    14161 ttgagctttc gctttttcaa cttcagcagt tgttgttgca tcagtaatac cttgtttagc
    14221 ttgatctgta atttgtttaa tcattgctaa tgcttcagtt ttttcttcag cagttaactg
    14281 gtcactatga tcaattgatt ctttcgtatc ttctgcttta gcttcaatag cttggttcgc
    14341 tttaggttta acagtagcat ctacttgaat agtatcaatt gctgctttac cttgtgttaa
    14401 tgcatcatca acgtcaccat tatccacacc attattaatg ttttctaatg cagtttgaac
    14461 attttggtca acttgcttaa ttgcttgttg cttttcatct tgtgttgcat tagtgtcagc
    14521 tgaaatatta tttttcttct gatctgcata agcatataaa tctgttgtag ctgattcttt
    14581 tttacctgtt ggaattgtgt aatcgttaat attatctaag tcattatgaa tttgaacttc
    14641 aatgtcatct ttagaagtag cttgattaac attttgatcc gcagtttgtt ttaattcagc
    14701 aagtttttgt ttcgcttcag caatttcact tgaagtcgat gcgttagagt tatccgcttc
    14761 gtttacttta gcattatatg catcttcaat tttagctaaa gcatcttttt tgtactcact
    14821 aaatgtttta actgcattaa ttttagcttt tccttcttta actgcattat caacatattc
    14881 atttgttgat gattgatcga catttgtttt cgcttgattt aattcagtat caacttcttg
    14941 ttttgcatca ttaatttctt gttgtgatgc atttggtgtt tgttctattt gtgttttctt
    15001 atcagctgca gcttgatcta attctttttt acctgctggt ttcttaacag gatttgcatg
    15061 aagttgttga actttttgta ctgctgtatc tttagcagta gttacatcac ctgtagtagt
    15121 tgctgcatta atattattta aaccttcttc atatgctgct ctaactggtc caatatcgtt
    15181 acctttttct tcattagtag tctcattatt attaagtatt tcagttattt tattttgcat
    15241 ttaagttagc aattctgctc ttgcattcgt tttaacatct gttgatgctt gaattgggtc
    15301 aattgcttga attgcattat cttttgcagt gtttacatca tcgattgact gtgcattttc
    15361 aatattttga ttaccttgtg ttaattgtgc gtctacttgt tgatttgctt gttctttttc
    15421 ttcagtagtc gcatctgcag tttgtgcaat aagcgctttt tgttcgtttg cttttgttgc
    15481 taattcatct ttcgcaacat ctttaattgt tgtatctgca gtaatacctt gaatatcagc
    15541 aactgcttga tctttaattt gcgtaacatc attagttgtt tgtgcattta agatatcttg
    15601 atacgctttt tctttagctt ttaaaactaa atcttttgct gcatttttct cttcagttgt
    15661 agcgccagtt gtattatcaa ttgcttgatt ttgagttgtc acagcttgat caacttcatt
    15721 tttcgcattc gatttaactg ctgttgctgg ttgtgtgctt tgaattgaat tctttccagc
    15781 gtctttcgct gtatctacac cattatcatc agttgcagct gtaatattat ttttcgcgtc
    15841 tgtaactgct gttgctaatt gttgaatagc tgcttctttt tcttcatttg tagcgttctg
    15901 atcattattg ataacatttg tttgcgttgc ttgtaattga tcaatctcat ctttagccgc
    15961 ttgtttctta acaacttttg gtgttaccgc attaattgct gcttctgcat tagctttcgc
    16021 ttcatcaact tgtgcgttag tagttgctgc tgaaatggct tgatttgctt taccattttc
    16081 agtatttgct tctgcatcag ctgcttgttt ttcttcatct gttgcatctg gtgtagcttg
    16141 aatctcttgc aatttgttat ttaaaattgc tgtgatttca ttacgtgcag ttgctttttt
    16201 attaactgtt ggtgttactt gatcaatact agtttcacct gtcgttttcg cttggtctac
    16261 atcattttga ctattagcag cttcaatgtt gctatttgct tgtgtcacag cattatctac
    16321 gtccgcatta gccgctgcaa tttcttcagc agtaatatct tgcgtttgag cgattgctgt
    16381 tttacgttca ttcgctttcg tagcaattgc ttgtttcgca ttatctttag ttgttgttgc
    16441 tggctgaatc gcttcaattt tagcaatttc tgcattttta gccgcttcaa cttccacatt
    16501 tgaatgagct gcatctattg ctgcatcagc agccgttttt tcagtttgaa cttgttgttt
    16561 agcagcttct ttttcttctg ttgttgaacc gttattagca tcaattgctg tttcttgagc
    16621 ttgtacttta tctgcaattg cttgttttgc tgctggttta acatttgcat ctggcgtaat
    16681 tgcagctatt gtagcttcat tagtagtttt tgcattatct acatctgtat ttgctgtagc
    16741 attatctata tcagcgtttg cagtaactac tgcttgatca actttatctt ttgcagcttg
    16801 ttgttcttca gtagtcgaat cattcattgc ttcaattgca gttttacgtt cacttgcttt
    16861 ttgagcgatt tccgctttag catccgattt cttagttgtg gcagcttgca cttgattaat
    16921 tgcagcaata ccattgtctt tagctgttgt tacatcactg tttgtatttg cagcatcaag
    16981 atttgttctt gcttcttgct ttttagcatc taattctgta tatgcagctt ctttttcttc
    17041 tgtagttgaa gcattgctat tttggatttc ttgtttacgt gtattatatg cgttttctac
    17101 ttctgtatca gctgcaggtt taacttttgc ttgtgtttga attgcattga ttttatctaa
    17161 tacttttgct ttagtatcag ccacttcttg ttgtgattta acaacttgga tgtcatgtaa
    17221 accttctgtt tgagctgcat ctactgctgc atttgcctct gctacttctt catcagttgc
    17281 atttgaaact gtagcatttt gcgcttttct agctgtagct gcttgtctaa cttctttata
    17341 agcatccatt ttagtttgag ttgaatccgt aatattctca attttgaata tttcttcgtt
    17401 tttaacgcgt tctaaatctt gtgcagtcgt tgtcgcttca attgctttaa caccagaaac
    17461 tttagctgca ttaatacgtt caatagcttc cgctacttct tcatttgttg tatcaggatt
    17521 taaaggtgct tgatcaattt gtgcttgaac aacttcgtca aattcttcaa gagctgctgc
    17581 tttagctgtt gtagcaggtg tagtttgatt aatatcattg attgcttttg ttttaatggc
    17641 ttctacttgt gcatttgttg ttgctgcatc aatatcttta attgcctttg tttcaatttt
    17701 accaatttta tcatttgcta catctttttc atcttgtaat gatgcatttg actttttaat
    17761 ttgttgttta cgagttgtaa ctgcttggat aatatcttgt ttcgcttgag gtttaactgt
    17821 tggtgtaatc acatcttgtt ctaacactgc gataccatta tctttttcag ttgtgacacc
    17881 ttgtgatgtt gtttgtttat taatttctgc gattgcatta tttttatcat tgttaatttt
    17941 agtcacaagc gctgcaactt catcttgcgt aactttttta ctttgttgtg ctgctgtaac
    18001 cttttcttga gctttagtat ctaattcagc aattgctgct tgattatcaa ttaatgcagc
    18061 ttgtaattta gttactaaac cgtcaatatc cgcttgagat gcacgatttg cagtttcaac
    18121 gtgattcgca tcttcattta aaatagtgtc tgcttgttgt ttaagtttat tgtattctgc
    18181 aattgatgct gttgtgtaat gactattatc aacttgtgag tttacttgtt gttgcaacgc
    18241 atctttgttc atttcaaccg ctactgaaaa tggatctgca gttagtgtaa cttctggttg
    18301 cgcattatta attacaacat ctgaagcagt acgatatgtt aatttttcat taaaatcaat
    18361 atttttaggt gtatcgatat tcgcaacatt aactttatat gataatttta aagatttatc
    18421 tgggaataaa acttctttag tgtgtgtacc acgtgccgtt gtcacacctt ggcttgtaaa
    18481 tgtaacttta tttgcagttt gatcataatt aacagtcata tttttcaata ctgtactgtc
    18541 ttcattacca ttagggaatg ttgtagttaa tgaatcatta acgtaagtta caccttgtgg
    18601 taatgttact tcatatttaa attgatctgc ttttaatgaa gcaccaaaat taccattgtt
    18661 accgatttca gtattaatag tatattcttt attgttagtt gctttcggtg ctaaatctct
    18721 atttttgaca tataaatgtg atcctgagaa tagacctaca ttatcaatga atgaatagta
    18781 tttataacca tctttatttg ttgtaattct tgaagcatca gcaactgctg tattgtcagg
    18841 aataaattgt aaatctaaac gatcagcatc agttggtact ttaaataaat gtgcagtacc
    18901 agtcttagca acatttacag ttgctaattc agtatttttc ttagcatctt taataactaa
    18961 atttgttgca ccttgcttac tattcgtttg catagtagta aagttaataa ttaattctga
    19021 atctggtttt acatttacag tttttgtaat accgttaaag ttaccatgat ctgtagaatc
    19081 atttgcattc acacgaccta atgcagccac gtttccttta gcttgatagt tttgagggtt
    19141 attcttatca aacatatcgc ttcgtcttaa ttctgagtta acgaaaccaa tcttaccgtt
    19201 gttaattaat gaataaccat ttactttatc tgtaacagtt acagttggat cctgtctatt
    19261 ctcatctgtt gatatggcag gatcatcaaa tgttaatgtc gtattaatac tgccttcacc
    19321 agtattgcta gcatttggat cttgagtttg tgcgtttgct gctacaggtg ctgctggttg
    19381 cgctgctgct ggagcattcg ctggctgtgt ttgatttgcc ggtgttgcat tattattagg
    19441 tgttgcttgg ttatttcctt gacctgcttg gtttgccggt gctgcttgat ttccaggttg
    19501 tgcttgtgca gcgttattcg gatcagcttg attaccttgt ccagctggtt gtgctgctcc
    19561 tcctgctgga ttagcctgtc caccttggtt tgctggctgt gctgcaggat tagcttgagc
    19621 accagcattt gcgttaggct gtgtattggc atcagctggt tgtgctggtt gattttgtgc
    19681 aggctgattt tgctctgctg cagacgctgt tgtcgggtta gtagatataa aagtaacagt
    19741 ggcaattaaa gctgaaaaaa taccgacatt aaattttctg atactaaatt tttgttgtct
    19801 gaataaattc attaagtcat cctcctggtt gattattctc gctgttaaat gatttcactt
    19861 aatcaactgt taagataagt agtagcatct gcgttaaaaa cacaaagcaa ctctatctaa
    19921 ttaaaattaa ttttatcatc attatatatt gagtaccagt gtattttata ttacatattg
    19981 attactttgt ttttattttg tttatatcat tttacgtttg tactataaat tatttctaga
    20041 aacacaaaaa accgatgcat acgcatcggc ttatttgtaa tacagtattt atttatctaa
    20101 tcccatttta tcttgaacca catcagctat ttgttgtgca aatctttcag catcttcatc
    20161 agttgctgct tcaaccatga cacgaactaa tggttctgtt ccagaaggtc ttactaaaat
    20221 tcgaccttct ccattcattt ctacttctac tttagtcata acttctttaa cgtcaacatt
    20281 ttcttcaaca cgatatttat ctgttacgcg tacgttaatt aatgattgtg gatatttttt
    20341 catttgtcca gctaattcac ttagtgattt accagtcatt tttattacag aagctaattg
    20401 aataccagtt aataaaccat caccagttgt attgtaatcc atcataacga tatgcccaga
    20461 ttgttctcca cctaagttat aattaccgcg acgcatttct tctactacat atctgtcgcc
    20521 aactttagtt ttattagatt taattccttc ttgttcaagc gctttgtaaa aacctaaatt
    20581 actcataaca gtagaaacaa tcatgtcatt attcaattct tgatttttat gcatttcttg
    20641 accaataata aacataattt ggtcaccgtc aacgatttga ccattctcat ctactgctat
    20701 gattctgtct ccatcgccgt caaatgctaa cccaaaatca ctttcagttt caactacttt
    20761 ttcagctaat ttttcaggat gtgtagagcc acatttctca ttgatattat atccatcagg
    20821 actacatcca attgtttcag tatctgcttc taaatcacca aataagaatg gcgctagtga
    20881 tgatgttgaa ccatttgcac catctaaaac aattttcaaa ccttcaaagt taacatctac
    20941 tgttgatttt aaatagctca aatatttttg tgccccttca aagtaatctg aataatgtac
    21001 aatatcattg ccaactggtc ttggtaattc tggattttct tgatccaata atgcttcaat
    21061 ttcattttct tgttcatctg atagtttaaa accatctgat ccaaagaatt taataccatt
    21121 atctgcaact ggattatgag aggctgaaat cattacacct aactctgcac ccatatcgcg
    21181 tgttaaatat gcaacacctg gtgttgaaat aatacctaat cgcatcactt ctgcaccaat
    21241 tgaaatcaaa ccagctatta atgctgattc taacatttca cctgaaactc tagtatcgcg
    21301 acctacaagt acacgtgggt gtttttcacc tttattatgt gctagaacat agccaccgta
    21361 tcttcctaat ttaaatgcca attcaggtgt tagttcttgg tttgcgacac ctcttactcc
    21421 gtctgtacca aaatattttc ccattgttta ctctccttta atttagctat ttattttaca
    21481 tttatataag ccttcgtttc acttggttgt gctttagtga catgttctgg taaattgatt
    21541 tttacagtct tttcagttga ttctgaaata ccatctaaat ctacttctgc atcaacttcg
    21601 cttatatttt gtaagtcatc tcgactaccg aagatttcaa tttctttatc ttctaaatca
    21661 atcgaactta actctttatc atctgctaaa ctacctttct gtttaacatt tactttaacc
    21721 tttttgctaa aaggctctac tttaacttgt aaattcactt cattaggttg aatcgataca
    21781 tttaatttat tcagtttttt atcaaaagcc gttacttctg cgacatcttt tgtgtcacca
    21841 ttaatctttt tattagtttt aaaagtggct tttaaataag cgatatcatt caattgttct
    21901 tctccacctg ttactttaac tgtttgtggt gaaacttctt gctttgtaat tttataaagt
    21961 ggatcaatat cactctgact tacatcaggt tgaactgtca tctttttagt tactttgttt
    22021 tcaagcgtaa tatttgctaa tttaggtttt acagaataat gaatgtcatc agctaaccct
    22081 ttaacttgat acttctcttg atattttcca gctttagcat ttgataaatc aatcactact
    22141 cttaaatctt ctggattttc aatttttatt atctttgatt gtggtcctga aatagtcaca
    22201 ttaactgttt caggcgcttt tgttaaatgc aaatctttag tgttataaag aatttcaacg
    22261 ggtacatctt gaatcgtttt actagacttt tgaccaagat taccagtgtt aaagatattt
    22321 ccaaaaacat tgttaacaga taaaaagaaa aacaatgcca aaagaaaggc aataaatctc
    22381 aagccccatt tactttctag catattattt cacacctttc ttttgaaagc gtgtgccaaa
    22441 ccaatgttca gcaagcaact cttcaaaaat ttcgtttgaa atgtctcgtc gtaattttcc
    22501 atcaaatgtt accgaaatat caccagtttc ttcagataca ataacggtaa atgcatcaga
    22561 tacttctgaa ataccaaccg cagctctatg tcttgtaccc aaacttttag atatcttagg
    22621 actatcagac aatggcaaat aacttgctgc tgctgcaatc ttagtgcctt gaataatcat
    22681 tgcaccatca tgtaaaggtg tgttaggtat aaagacatta attaaaagtt cttgcgaaat
    22741 atttgaatcc attgcaatac ctgtttcaat ataatcttga agacctgttt ctttttcaaa
    22801 gacaattaat gcacctatac gtcttttagc catatattgc acagcctttg aaaccgattg
    22861 aatcaatttc tcttcatctt tactatacgt attagaagta tagcgtttta aaaagctacc
    22921 tctaccaagt tgttctaacg cacgtctaat ttctggttgg aatattacta ttaaagctaa
    22981 taccccccat tgaataacga tatcgaataa tttagatgtt gcagtcaagt tcaatatcat
    23041 acttatctgc tgaccaataa caattactaa tatcccttta agtaattgta tcgctttagt
    23101 tcccttaaag accgtgatga gaagataaag tacataccaa actatcagta aatcaaggat
    23161 actcgttaca atttttaacg tactgaggtt ttgaaaaaag ttggaaaaat ccataacatc
    23221 tcctccgggt aatatttttc cataataccc attataccaa tcatttacat cactgtcata
    23281 cgaacatata tttaaataga aaaaatttat tttaaagatt ataactactc ttaatcattt
    23341 tagtgaatta aaaaaagtag tgcaaaaagc aaaatatact ttatacacta caaatcattt
    23401 atttataata aagtttcacc aaaaaatgtt ccaactaatg aaaccgcttg ttcagcagta
    23461 tgattattac tgtcaatcaa tggatttact tcaactaaat ccattgagga aattaaatgt
    23521 gattgatgca gtaattccaa tgcaaaatgg ctttctctat aactaagacc acccaaaact
    23581 ctagtaccag tgcctggcgt ttcaagcgga tctaaagcat caacatctaa agataaatga
    23641 acgccatcaa cattgcgtga cttcaaatat tctattgtat tttcaattac ttcctttatc
    23701 cctaatttat caatatctga catagtaaat gttttaatat tatgatcttt gataaattgt
    23761 ctttcacctt tatctaaatc tctcatacca attagtacga tgttttctgg cttgattaca
    23821 ttactattta attctaaaag ttctttggga ccttcgcctg tcaaaatcct tagaggcata
    23881 ccatgaatat ttccacttgg tgactcttca ggtatattta aatcaccatg tgcatcatac
    23941 caaataacac ctaaattatt ataatgttta cttattgctg atactgaacc taccgcaata
    24001 gaatgatcac caccaagaac tagaggaaat ctgttatttt caattgatgc tgaaacctct
    24061 ttatttaatt tttgattaac atctataatt tcatcataat ttcttaatcc tttttgttca
    24121 ctatgaaatt tttcaatgtt cacagcaggt accttaatat cccccttgtc atatacatca
    24181 aggtctaatt gctttaatct tgaaattaat ccagcatatc taattgctgt tggtcctaaa
    24241 tcaacaccta attttctttg tccaaatgtt gatggtgcac ctataatatc aattgctttt
    24301 gtctttgtca taagcgtccc cctttgctct atgtaattaa agaataatgt atgcgcttac
    24361 cattatcaag caatagctac acatataatc tgtttatctt attacttcat aaaaaaaagc
    24421 acttcccaaa aatggaaagt gcaagtagtg agccatagag gattcgaacc tctgaccctc
    24481 tgattaaaag tcagatgctc taccaactga gctaatggct ctaatggctg ggctagctgg
    24541 attcgaacca acgagtgacg gagtcaaagt ccgttgcctt accgcttggc tatagcccaa
    24601 tatatagatg gtggaggggg gcagattcga actgccgaac ccgaaggagc ggatttacag
    24661 tccgccgcgt ttagccactt cgctacccct ccagcttatt catataattt aataatcaaa
    24721 atggtggaga atgacgggtt cgaaccgccg accctctgct tgtaaggcag atgctctccc
    24781 agctgagcta attctccaaa ataatgactc ctacgggact cgaacccgtg ttaccgccgt
    24841 gaaagggcgg tgtcttaacc gcttgaccaa ggagccatgg ctccacaggt aggactcgaa
    24901 cctacgaccg atcggttaac agccgatagc tctaccactg agctactgtg gattaatatt
//
LOCUS       DAHHSC010000019        24112 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.019, whole genome shotgun sequence.
ACCESSION   DAHHSC010000019 DAHHSC010000000
VERSION     DAHHSC010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 24112)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24112)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24112
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.019"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            complement(<1..355)
                     /locus_tag="NBI57_000603"
     CDS             complement(<1..355)
                     /locus_tag="NBI57_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCD1821572.1"
                     /translation="MAINIIEYNRSYKEELIEFILSIQKNEFNIKIDRDDQPDLENIE
                     HNYLNSGGQFWLAINNHQNIVGTIGLIRLDNNMSALKKMFVDKGYRNLKIGKKLLDKV
                     IMTCKEQNIDGIYLGT"
     gene            complement(631..1332)
                     /gene="isaA"
                     /locus_tag="NBI57_000604"
     CDS             complement(631..1332)
                     /gene="isaA"
                     /locus_tag="NBI57_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase IsaA"
                     /protein_id="HCD1821573.1"
                     /translation="MKKTIMASSLAVALGVTGYAAGTGHQAHAAEVNVDQAHLVDLAH
                     NHQDQLNAAPIKDGAYDIHFVKDGFQYNFTSNGTTWSWSYEAANGQTAGFSNVAGADY
                     TTSYNQGSDVQSVSYNAQSSNSNVEAVSAPTYHNYSTSTTSSSVRLSNGNTAGATGSS
                     AAQIMAQRTGVSASTWAAIIARESNGQVNAYNPSGASGLFQTMPGWGPTNTVDQQINA
                     AVKAYKAQGLGAWGF"
     gene            complement(1940..2995)
                     /locus_tag="NBI57_000605"
     CDS             complement(1940..2995)
                     /locus_tag="NBI57_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIC"
                     /protein_id="HCD1821574.1"
                     /translation="MTSIKTITPKDFIFRVLSGVAIGIVAGLVPNAILGEIFKYFMDY
                     HPIFKTLLGVVVAIQFTVPALIGALVAMKFDLSPLAIAVVASAAYVGSGAAQFKNGAW
                     MITGIGDLINTMITAAIAVLFILLIQHRVGSMALIVFPTVVGGISGAIGVLILPYTKM
                     ITTAIGNMVNGFTELQPIVMSILISMVFSLIIISPLSTVAIAFAIGITGLAAGSASIG
                     ISATEAVLIIGTSKVNRLGVPLSVFFGGVKMMIPNMVKYPILMLPILTTAIVSGLVSA
                     LVGIHGTKESAGFGFIGMVGPINAFKFMEVDSAWLSVLLIVVAFFVVPFVTAWLADII
                     YRKVFRLYTNDIFKFMG"
     gene            complement(3122..3250)
                     /locus_tag="NBI57_000606"
     CDS             complement(3122..3250)
                     /locus_tag="NBI57_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1821575.1"
                     /translation="MDFKNEDKIKVYCNNMFEKLPISLSKNSEFCDILFFIVLSYR"
     gene            3405..3974
                     /locus_tag="NBI57_000607"
     CDS             3405..3974
                     /locus_tag="NBI57_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCD1821576.1"
                     /translation="MMESKSIDPRIVRTKQLLVDAFLKISREKKLSQITVKDITDIAT
                     LNRATFYAHFTDKEDLLDYTLSVTILKDLNDNLSISNVINEKVLRNIFISIASYIKDA
                     AKSCELNSEAFCNKAHQRINNELEDIFAIMLENSYPEHQRDIIVNSASFLAAGISGLA
                     LHWFNTSQETADVFIDRNLPFLIHHIAHF"
     gene            4172..4672
                     /locus_tag="NBI57_000608"
     CDS             4172..4672
                     /locus_tag="NBI57_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1821577.1"
                     /translation="MTSIFVFAYLNNTSGNMLFRQQMICACLLANVRHSSIVCLNNIC
                     CIVDMRCLNTKIGGIDVSETDEPQGFERTHNILNINQSSLGVVTYITNKLKSTLKQHI
                     IIARGKKRIDYRLSYNFYTTYYDNVEIKKIDCEYTYAMPIAILIRHTMSFDNAHFFAQ
                     LRHPVL"
     gene            4892..5122
                     /locus_tag="NBI57_000609"
     CDS             4892..5122
                     /locus_tag="NBI57_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 domain-containing protein"
                     /protein_id="HCD1821578.1"
                     /translation="MKILDRINELANKEKVQPLTVAEKQEQHALRQDYLSMIRGQVLT
                     TFSTIKVVDPIGQDVTPDKVYDLRQQYGYIQN"
     gene            complement(5424..5801)
                     /locus_tag="NBI57_000610"
     CDS             complement(5424..5801)
                     /locus_tag="NBI57_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCD1821579.1"
                     /translation="MIQSMWFNLHVQDLEKSAQFYKALGFKINRNPQMLDKMVGIQIG
                     QTTVILIENKHFQNVSQQSLNTEPNEVMISLGVNTNEEVDQLVNKVKEAGGAVVQEPT
                     VSQGFYGAMFKDLDGHHFNFLVC"
     gene            complement(5818..6637)
                     /locus_tag="NBI57_000611"
                     /pseudo
     CDS             complement(5818..6637)
                     /locus_tag="NBI57_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501348.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NmrA/HSCARG family protein"
     gene            complement(6657..6956)
                     /locus_tag="NBI57_000612"
     CDS             complement(6657..6956)
                     /locus_tag="NBI57_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2316 family protein"
                     /protein_id="HCD1821580.1"
                     /translation="MSLNKEQRRITAEELQAHFEESTLSVQMIAEKLNVTTEDVEKVL
                     AMTAPLGIFSHQLQRFIHLVWDVRDVINDNIKGNGQTPEPYTYLKGEKEDYWFLR"
     gene            7087..7644
                     /locus_tag="NBI57_000613"
     CDS             7087..7644
                     /locus_tag="NBI57_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCD1821581.1"
                     /translation="MRKDAKENRQRIEEIAHKLFDEEGVENISMNRIAKELGIGMGTL
                     YRHFKDKSDLCYYVIQRDLDIFITHFKQIKDDYHSNYEVMQVSLDYLLQFKIDNKALL
                     QCIEAGNNKLRFYQSAFYQELFDFYYDLFKSDDDTYTKFKTDMLLQSLSTSVFAFQIE
                     HRHISIEAYRNYLLNIYLDEVERND"
     gene            7637..8341
                     /locus_tag="NBI57_000614"
     CDS             7637..8341
                     /locus_tag="NBI57_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HCD1821582.1"
                     /translation="MTKIVLITGGNKGLGYASAEALKALGYKVYIGSRNDVRGQQASQ
                     KLGVHYVQLDVTSDYSVKNAYNMIAEKEGRLDILINNAGISGQFSAPSKLTPRDVEEV
                     YQTNVFGIVRMMNTFVPLLEKSEQPVVVNVSSGLGSFGMVTNPETAESKVNSLAYCSS
                     KSAVTMLTLQYAKGLPNMQINAADPGATNTDLVGDFSNNSKHVSEGIKPIIQLATIGA
                     DGPTGTFINGDGEMPW"
     gene            8439..9449
                     /locus_tag="NBI57_000615"
     CDS             8439..9449
                     /locus_tag="NBI57_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HCD1821583.1"
                     /translation="MKSITFEEHYVIEDIQKETMNAISADPKGVPMKVMLEGLEKKTG
                     FTNADELSHHDERIQFMNNQDVQIQVLSYGNGSPSNLVGQKAIELCQKANDQLANYIA
                     QYPNRFVGFATLPINEPEAAAREFERCINDLGFKGALIMGRAQDGFLDQDKYDIIFKT
                     AENLDVPIYLHPAPVNSDIYQSYYKGNYPEVTAATFACFGYGWHIDVGIHAIHLVLSG
                     IFDRYPKLNMIIGHWGEFIPFFLERMDEALFAEHLNHSVSYYFKNSFYITPSGMLTKP
                     QFDLVKKEVGIDRILYAADYPYIEPEKLGVFLDELGLTDEEKEKISYTNGAKLLGLSS
                     NN"
     gene            9469..10299
                     /locus_tag="NBI57_000616"
     CDS             9469..10299
                     /locus_tag="NBI57_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCD1821584.1"
                     /translation="METLELQGAKLRYHQVGQGPVLIFIPGANGTGNIFLPLAEQLKD
                     HFTVVAVDRRDYGESELTEPLPDSASNPDSDYRVKRDAQDIAELAKSLSDEPVYILGS
                     SSGSIVAMHVLKDYPEVVKKIAFHEPPINTFLPDSTYWKDKNDDIVHQILTEGLEKGM
                     KTFGETLNIAPIDAKMMSQPADTEEGRIEQYKRTMFWSEFEIRQYTHSDITLDDFTKY
                     SDKITLLNGTDSRGSFPQDVNFYINKETGIPIVDIPGGHLGYIQKPEGFADVLLNMWG
                     "
     gene            complement(10446..11336)
                     /locus_tag="NBI57_000617"
     CDS             complement(10446..11336)
                     /locus_tag="NBI57_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HCD1821585.1"
                     /translation="MKIVIIGGFLGGGKTTVLNHLLAESLKESLKPAVIMNEFGKMSV
                     DGALVSEDIPLSELTEGCICCAMKADVSEQLHQLYLKEQPDIVFIECSGIAEPVSVLD
                     ACLTPILAPFTTITHMIGVIDASMYKHIKSFPKDIQGLFYEQLAYCSVLFVNKIDSAD
                     VETTSKLLKDLEVINPEADIQVGMHGSVTLPISVRQMTATSDNKHESLHKTINHQFVQ
                     SPVTCTKAEFIKRLACLPSHIYRLKGFMTFEDTTHTYLIQFTQGQYELTPVAFSKKVP
                     EYLVLIGKGISKEDYQCLEQ"
     gene            complement(11433..12773)
                     /locus_tag="NBI57_000618"
     CDS             complement(11433..12773)
                     /locus_tag="NBI57_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000895187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter B"
                     /protein_id="HCD1821586.1"
                     /translation="MLEVKYDMRTGHYTPIPHEPHYLVISHADKLTATEKAKLRLLII
                     KQKLDISLAESVVSSPIASEHVIEQLTLFQHERRHLRPKISATFLAWLLIFLMFALPI
                     GIAYQFSDWFQNQYVSAWIEYLTQTTLLNHDILQHILFGDYGVLSLGTYSLVWALPVV
                     ILISLSTAIIDQTGLKSWMIWAIEPSMLWIGLQGNDIVPLLEGFGCNAAAISQAAHQC
                     HTCTKTQCMSLISFGSSCSYQIGATLSIFSVAGKSWLFMPYLILVLLGGILHNRIWLK
                     KNDQQLSVPLPYDRQLHMPNIRQMLLQMWQNIQMFIVQALPIFITICLIVSILSLTPI
                     LNVLSQIFTPILSLLGISSELSPGILFSMIRKDGMLLFNLHQGALLQGMTATQLLLLV
                     FFSSTFTACSVTMTMLLKHLGGQSALKLIGKQMVTSLSLVIGVGIIVKIVMLII"
     gene            complement(12767..13873)
                     /locus_tag="NBI57_000619"
     CDS             complement(12767..13873)
                     /locus_tag="NBI57_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HCD1821587.1"
                     /translation="MQHHKVAIIGAGAAGIGMAITLKDFGITDVIILEKGTVGHSFKH
                     WPKSTRTITPSFTSNGFGMPDMNAISMDTSPAFTFNEEHISGETYAEYLQVVANHYEL
                     NIFENTVVTNISADDAYYTIATTTETYHADYIFVATGDYNFPKKPFKYGIHYSEIEDF
                     DNFNKGQYVVIGGNESGFDAAYQLAKNGSDIALYTSTTGLNDPDADPSVRLSPYTRQR
                     LGNVIKQGARIEMNVHYTVKDIDFNNGQYHISFDSGQSVHTPHEPILATGFDATKNPI
                     VQQLFVTTNQDIKLTTHDESTRYPNIFMIGATVENDNAKLCYIYKFRARFAVLAHLLT
                     QREGLPAKQEVIENYQKNQMYLDDYSCCEVSCTC"
     gene            complement(14206..14502)
                     /locus_tag="NBI57_000620"
     CDS             complement(14206..14502)
                     /locus_tag="NBI57_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4176 domain-containing protein"
                     /protein_id="HCD1821588.1"
                     /translation="METIGSIIYLKEGSQKLMIINRGPIVEIENQKYMFDYSACKYPI
                     GVVEDEIYYFNEENIDSVIFKGYSDQDEVRFQELFENMKQNLDSEIQRGEVTPQ"
     gene            complement(14490..14858)
                     /locus_tag="NBI57_000621"
     CDS             complement(14490..14858)
                     /locus_tag="NBI57_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1821589.1"
                     /translation="MSNKLDGINKMITAKHKQMDDLYDEKREVKALIDESDELNHSIE
                     QLYQHLGDRYHSSNMASRMEQFRDEFHFAKRRSTEALYEQQQQIQHGIRKAEEEMIDL
                     EMRRNVEIETVTKEENKWKQ"
     gene            complement(14873..15151)
                     /locus_tag="NBI57_000622"
     CDS             complement(14873..15151)
                     /locus_tag="NBI57_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04197 family type VII secretion effector"
                     /protein_id="HCD1821590.1"
                     /translation="MMSTVQSDIFKTNSASSSIKSAVETCNNVSKPDKDESTTVSGNN
                     NAHSVIDDLISKNQSVAEAIRNASDSIQKVGEEFNQTDVMIGNEIGKN"
     gene            complement(15492..16358)
                     /locus_tag="NBI57_000623"
     CDS             complement(15492..16358)
                     /locus_tag="NBI57_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HCD1821591.1"
                     /translation="MNEQWLEHLPLKDIKEISPVSGGDVNEAYRVETDTDTFFLLVQR
                     GRKESFYAAEIAGLNEFERAGITAPRVIASGEVNGDVYLVMTYLEEGASGSQRQLGQL
                     VAQLHSQQQEEGKFGFSLPYEGGDISFDNHWQDDWRTIFVDKRLDHLKEELLNRGLWD
                     ANDIKVYDKVRSQIVEELEKHQSKPSLLHGDLWGGNYMFLKDGRPALFDPAPLYGDRE
                     FDIGITTVFGGFTSEFYDAYNKHYPLAKGASYRLEFYRLYLLMVHLLKFGEMYRDSVA
                     HSMDRILQGTTS"
     gene            16576..17640
                     /locus_tag="NBI57_000624"
     CDS             16576..17640
                     /locus_tag="NBI57_000624"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HCD1821592.1"
                     /translation="MYKLIKPFLFKIEPEKAHGLTIDALKTLQKFPVLFPVVDKLFTY
                     KNPTLSQTIQGNTYDNPIGLAAGFDKSCEVPKALEHLGFGALELGGITPKPQPGNPQP
                     RMFRLLEDDALINRMGFNNIGMNKALSHLRKNAYQVPVGINVGVNKMTPYEARYQDYI
                     KVIDTFKHDVSFFTVNISSPNTENLQNFHDKDEFSMLCQALTTFKKQHDVTVPIYLKL
                     TSDMDFDGLKALLPAITETFDGIILANTTRQRDGLTSANKVEEGGLSGRPLFERNLKL
                     IKYAYQQTNGEFLIIGTGGVFSTEDAIKMMRHGASLIQIYSSLVIEGPGLTKKMNKGI
                     ARYLKDHHFDNVSDIIGLDA"
     gene            17795..18070
                     /locus_tag="NBI57_000625"
     CDS             17795..18070
                     /locus_tag="NBI57_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1821593.1"
                     /translation="MTKETDNKKTVATSDNKDTQQQNQTEEDERQGLNGYRKTDLDLE
                     IEQELREMMKTGENETKNDYKKFKVFSLISTLVIVILAIIRFVHKMI"
     gene            complement(18256..18978)
                     /locus_tag="NBI57_000626"
     CDS             complement(18256..18978)
                     /locus_tag="NBI57_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HCD1821594.1"
                     /translation="MINAEPIISKMKNQKINYDKVLKKLIGQWEREAIRPKILLHSCC
                     APCSTYTLEFLTQYADIAIYFANSNIHPKNEYLRRAKVQEQFVEDFNRKTGANVKYIE
                     APYEPHKFVKMVKDKELADEKEGGLRCTACFEMRLDIVAKAAVEHGYDYFGSAITLSP
                     KKNAQLINELGMDVQKIYDVNYLPSDFKKSKGYERSIEMCNDYNIFRQCYCGCVFAAM
                     QQGIDFKTVNKEAKAFLEQYPD"
     gene            19332..19781
                     /locus_tag="NBI57_000627"
     CDS             19332..19781
                     /locus_tag="NBI57_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase/bleomycin resistance/extradiol
                     dioxygenase family protein"
                     /protein_id="HCD1821595.1"
                     /translation="MTALFPYIAFENSKEALAYYEEVFGATDVKRLEVGEEQASHFGM
                     TKEEAQEATMHAEFEVLGVKVLCSDSFGRADKINNGISLLIDYDVNNKEDADKVEAFY
                     EKIKDHSSIEIELPFADQFWGGKMGVFTDKYGVRWMLHGQDYTAIQQ"
     gene            complement(19862..20449)
                     /locus_tag="NBI57_000628"
     CDS             complement(19862..20449)
                     /locus_tag="NBI57_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCD1821596.1"
                     /translation="MTIDRRIRKSQVAMRNAFIELLHQHQLEEITVQQIADLADVNRS
                     TFYTHYYDKYDLLEKLEDQQLEEIRDFIHDEKLNGAVKLSTDNIHQIMTVLIDMIEKD
                     IAFYQLMFRMGKDSNIHEKLYDLIMCHLQRYKNENDQINGIPFSYFMSYVSGAGLSLI
                     RHWVEDPNRIDKVDLIMHFNTIVNQGPATIIKNIQ"
     gene            complement(20427..20535)
                     /locus_tag="NBI57_000629"
                     /pseudo
     CDS             complement(20427..20535)
                     /locus_tag="NBI57_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501367.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            20592..22274
                     /locus_tag="NBI57_000630"
     CDS             20592..22274
                     /locus_tag="NBI57_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HCD1821597.1"
                     /translation="MNQHLLGNPKLTVTHVNEVKTGINHIVVDSVQYGNQEMIMEKDV
                     TVEMRDGEKLYINIFRPNKDGKFPVVMSADTYGKDNKPKITNMGALWPTLGAIPTSSF
                     TPEESPDPGFWVPNDYVVVKVALRGSDKSKGVLSPWSKREAEDYYEVIEWAAKQPWSN
                     GNIGTNGVSYLAVTQWWVASLNPPHLKAMIPWEGLNDMYREVAFHGGIPDTGFYRFWI
                     QGIFARWTDNPNIEDMIQAQQEHPLFDDFWKQRQVPLSQIKTPLLTCASWSTQGLHNR
                     GSFEGFKQAASEEKWLYVHGRKEWESYYARENLERQKSFFDFYLKEENNDWKDTPHVI
                     YEVRDQFYKGEFKSASAFPLPNTEYTPLYLNAENHTLNHAKISSAYVAQYDSEDKRQD
                     VSFKYTFDKDTELVGNMNLKLWVSTTDSDDMDLFAGIKKLDRRGNEVNFPDFNHIENG
                     QVATGWLRVSHRELDQEKSTIAQPWHKHETELKLSQDEIVPVEIELLPSGTLFKQGET
                     LEVVVKGSEIVIGNSTPGMKTRYEHEETINNGMHMIYTGGKYDSQLIIPIVN"
     gene            complement(22372..23007)
                     /locus_tag="NBI57_000631"
     CDS             complement(22372..23007)
                     /locus_tag="NBI57_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002955208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HCD1821598.1"
                     /translation="MKKVISLFVAILSVLIFTDNANAENNKPEGVNENSNLIPVRQPD
                     ANYPGPVSDIARVKKLDNLIDGTKDPDVIKQLKDDNEVPNNAVKAKDSKIVSDCRHLL
                     KHPDNLIDGTKDPNVINQLKNANGTSNNSAVNPSIKANDIHTKSNKQMAKQQSIHLDS
                     KSKSVHKQQLKELPKTGVNNESNTAIMALFIGLILLVVSLVTRFIKLNSHQ"
     gene            complement(23023..24099)
                     /locus_tag="NBI57_000632"
     CDS             complement(23023..24099)
                     /locus_tag="NBI57_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000161217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like serine protease"
                     /protein_id="HCD1821599.1"
                     /translation="MTKIFKISFLILLVVIVSSYYGESFVLADNNPIVNSENGSDNNF
                     ESGTIERNQNELYEVGDYSTPLTERVFGKDQRTVVNNILQRPYKQTVLLNMTFSNNRV
                     YKGTGTMIGKDIVLTAAHNVYSKDDKGWAKKIDVYAGVNGQTYTIGKAFSHKFFVSKT
                     WINNAPTKEDIAIIKLNSNLGNKTGYLTLNTHISKGENIEISGFPGDKSDNRQYKGKG
                     KLESFDENEMYYTVDTFSGQSGSAIRDSKNNIIGVHAYGRYNHNSGVRINDLKLDYIN
                     YWIGKYRSHPYNKKVKVIKSKYIYWKNIEITKKGDNKLIKKDKAYTAKLYYNIPNGYK
                     YYSLYDEKNRWMGYFNSNDIKVVK"
BASE COUNT         7897 a         4360 c         3714 g         8141 t
ORIGIN      
        1 ttgttcctaa ataaatacca tcgatatttt gttctttaca tgtcattata actttatcta
       61 aaagtttttt acctattttt aaattcctat aacctttatc aacaaacatt tttttaagtg
      121 cagacatatt attatctagt ctaatcaaac ctatagtacc aacaatattt tgatgattgt
      181 ttattgcaag ccaaaattgc cctccactat tcaaatagtt atgttcgatg ttctccaaat
      241 caggttgatc atctctatca atttttatat taaattcatt tttttgaatc gataaaataa
      301 actcgattag ctcttcctta taagacctat tatattcaat tatgtttata gccattttta
      361 tctccttttt catttaattt aatcataaaa tgcgcgttta gtttgtatct agtgtactca
      421 gtacagcctc aaatgaagtt ttattccact tggcacttaa taaagacaag tattttagca
      481 gtaatacaat aaagtccaat aaatttccct aacttcaata tccacttttt aagaaatgta
      541 tttttaatta ataaaaaaac tctccccaat ttctatggga agagctatat atttaatgtc
      601 taaacattac ttttatttat tatgaaggaa ttagaatccc caagcaccta aaccttgtgc
      661 tttgtatgct ttaacagctg cgttgatttg ttggtcaaca gtgtttgtcg gaccccaacc
      721 tggcatagtt tggaataaac ctgaagcacc tgatgggttg taagcattta cttgaccatt
      781 tgattcacga gcgatgattg cagcccatgt agaagctgaa acaccagtac gttgagccat
      841 gatttgagct gctgatgaac cagtagcacc tgcagtatta ccattgctta atctcactga
      901 acttgaagta gttgaagtgc tgtagttatg gtaagttgga gctgaaacag cttcaacgtt
      961 tgagttactt gattgtgcat tgtagcttac tgattgtaca tctgaacctt ggttgtatga
     1021 agtagtgtag tctgcacctg caacgtttga gaaaccagca gtttgaccat tagctgcttc
     1081 atagctccat gaccatgtag taccatttga agtaaagtta tattggaaac catcttttac
     1141 aaagtggatg tcatatgcac catctttgat tggagctgca tttaattgat cttggtgatt
     1201 atgcgctaag tcaactaagt gtgcttgatc aacgtttact tcagcagcgt gtgcttgatg
     1261 tcctgtacct gctgcgtaac ctgttacacc taatgccact gctaatgatg atgccataat
     1321 tgtctttttc atagtaaaaa atcctccagt aataattgta agtttatgtt tttagtaatt
     1381 atattttgaa tttgaatgtc gtagtgcaag tttaaattgt cttttatttc tttcaacggt
     1441 actcactata tcacaaaaaa ccagccagta aattacactt tctttacaaa acattacaat
     1501 atcaagtgtt atttgtaatg ttgaaatatg gctgttttat actgtaatgt gaaatatgtg
     1561 ccctttagaa tccaatcaac ccttgaaata gtctttaaca cataagattt ttactatatt
     1621 tagctcaact attacagctt tcgtaatatt acagattgta tttttgttac atagctgtaa
     1681 tatatctgac atgtaacttc tcttattttc gtacattaaa cgcaattaaa aagcaatcaa
     1741 cacatatgtt tctgcacatg tattgattgc tattattgtt gtatattcaa agttttaaaa
     1801 cacacatctt ttgtgaatta tcttatcttt tataagcaca aataaactgc aactcaatta
     1861 tattgctaaa ctttattctc gcaattcaca ataacattaa ataatttttg gtctaatatt
     1921 ttcaaaaaac atactgtcct tatcccatga atttaaaaat atcattagta tataaacgaa
     1981 acactttacg ataaatgata tctgcaagcc aagctgttac aaatggcaca acaaagaacg
     2041 ctactacaat tagtaagaca ctcaaccaag cagaatcaac ctccataaat ttaaatgcat
     2101 taatcggtcc taccattcct ataaaaccaa atccagctga ttctttcgtt ccatgaatac
     2161 ctactaatgc tgataccaaa cctgatacaa tggctgttgt caatattggt aacataagaa
     2221 ttggatattt caccatatta ggtatcatca ttttaacgcc tccaaagaag acggataacg
     2281 gcacccctaa acgattcact ttacttgtac caattatcaa tactgcttca gtcgcggaga
     2341 taccaattga cgctgatcca gctgctaaac ctgtaatacc tatcgcaaag gcaatggcca
     2401 cagttgatag tggcgaaata ataataagac taaataccat tgaaatcaaa atactcatga
     2461 caatcggttg taattctgta aaaccattaa ccatattacc gatggctgtt gtaatcattt
     2521 tcgtatacgg caatattaaa acaccaattg cacctgaaat accgccaaca actgttggga
     2581 atacaatcaa tgccatacta cctacacgat gttgaataag taaaatgaac aacactgcaa
     2641 tcgctgctgt aatcattgta ttaattaaat caccaatacc cgtaatcatc caagcaccat
     2701 ttttaaactg cgctgcaccg cttcctacat atgctgcact tgccacaaca gcaattgcta
     2761 atggcgatag gtcaaatttc atggcaacca atgcaccaat caaagcaggt actgtaaatt
     2821 gaattgcaac gacaacgcct aataacgttt taaaaatcgg atgataatcc ataaagtatt
     2881 taaaaatttc tccaagtatc gcattaggaa ctaaacccgc aacaatacct atggcgacac
     2941 ctgataaaac tctaaatata aaatctttgg gtgtaattgt tttaattgat gtcataatat
     3001 catccttcca tttatgtata tacatctgta tgcaaataat aaagagcctt aagttataag
     3061 ctgccactag cttaaattct aagatgtgca tgccgatgtt gttatattta ggctagcagt
     3121 atcatctata actcaagact atgaaaaata gtatatcaca aaattctgaa tttttagata
     3181 aagaaattgg caatttttca aacatattgt tacaatacac ttttatttta tcttcatttt
     3241 taaaatccat taatacaata gaagaaagac attcaaatgc ttaccaaaaa ggtacattat
     3301 ttgttaggag cgtatcaaca cttacatatc atcaacacaa ttgacaatat aatagaagat
     3361 actgataata agtgttaaaa caacagatgt taggtagtga acaaatgatg gaaagtaaat
     3421 ccatagatcc aagaatcgtt agaaccaaac aattgcttgt cgatgctttt cttaaaattt
     3481 ctagagaaaa gaaattatct caaattacag ttaaagatat cactgatatt gctacgctaa
     3541 atcgtgcaac attttacgct catttcactg ataaagaaga cctcctagac tacacattat
     3601 ctgtaaccat tttaaaagac ttgaatgata acttgagcat ttctaatgtc attaatgaaa
     3661 aggtgctgcg taatattttc atttcaattg cgagttatat caaagatgct gcaaagtctt
     3721 gcgaattaaa tagtgaagca ttttgcaaca aagcacatca acgtattaat aatgaattag
     3781 aagatatttt tgcgattatg ttagaaaaca gctatccgga gcatcaacga gatatcattg
     3841 taaatagtgc gagtttttta gcagctggta tctcaggctt agcattacat tggtttaaca
     3901 cgagtcaaga gacagccgat gtgtttatcg atcgcaacct tccattttta attcatcata
     3961 tagcacattt ttaataaaac ttggtattta gtcatgcatc ttgaaatcac tatgtgactt
     4021 aggttcatac ttgtacacac aataaaattt aacatattac gattgattag ccgtgtctag
     4081 gacataaatc aacgtcctat actctacaat gtcatattag cagtcgttaa ctgaatgaaa
     4141 ataagcttgt cattaaaaca tatagatttt agtgacaagc atttttgttt ttgcgtactt
     4201 aaacaacact tcaggcaata tgttgtttag gcaacaaatg atatgtgcgt gtttattggc
     4261 aaacgtacga catagtagta tagtatgtct aaacaacata tgttgcatag ttgatatgcg
     4321 ttgtttaaat actaagatag gagggattga cgtgagcgag acagatgaac ctcaggggtt
     4381 tgaacgcacg cataatatat taaatattaa tcagagtagt ctgggtgtag tgacatacat
     4441 tacaaataaa ttaaagtcga cgttgaagca acacataata attgctcgtg gtaaaaagcg
     4501 aatcgactat cgactgtcgt ataactttta cactacgtat tatgataatg tagaaatcaa
     4561 gaaaatcgac tgtgaatata cctatgctat gcccattgca attttaataa gacacacgat
     4621 gtcattcgac aatgctcatt tctttgctca gttacgtcat cctgtcttat aaaacaacat
     4681 tgcagacatg tatatcaaac gacacttcaa taacatcact ttgccatcgt actactatta
     4741 aaatcgtgtc tcaaacccct tattttaatt ccaaaaatct gctggtcaaa agaccgagaa
     4801 actaaaaaca ttactggatg tgttgataaa ttatcttata gaaataaact caaaatatat
     4861 caacacttga ttctaaggag gatatgacaa tatgaaaatt ttagatagaa ttaatgaact
     4921 tgcaaataaa gaaaaagtcc aaccacttac tgtagctgaa aaacaagaac aacatgcatt
     4981 gcgtcaagac tacttaagta tgatccgagg acaagtatta acaacatttt ccacaataaa
     5041 agtggttgat ccaatcggtc aggatgtcac accagataaa gtttatgatc ttcgccaaca
     5101 atacggttat attcaaaatt aatatttgct cacgaggtat tgcacttaag gtgccaactg
     5161 acctcataaa caaagcccat actgattgaa gacactaatg tgtccaggct gggacataaa
     5221 tcaatgttct atgctctacg aagttatatt ggcagtagtt gactgaatga aaatgcgctt
     5281 gtaacaagct tttttcaatt ctagtcaacc ttgccggggt gggacgacga aataaatttt
     5341 gcgaaaatat catttctgtc ccactcccac attaaacttc atctctgtat gggcttttta
     5401 tttattcttt tgagaatttc attttagcag actaaaaaat taaaatggtg tccatcaaga
     5461 tctttgaaca ttgcaccata aaacccttgg cttactgttg gttcttgaac gactgcgcca
     5521 cccgcttctt tcactttatt caccaactgg tcaacttctt catttgtgtt cacacctaga
     5581 gaaatcatca cttcatttgg ttcagtatta aggctttgct gacttacatt ttgaaaatgc
     5641 ttgttttcta ttaaaattac ggttgtttga cctatttgaa taccgaccat tttatctaac
     5701 atttgtgggt ttctatttat tttaaatcct aacgctttat aaaactgtgc gctcttttct
     5761 aaatcttgca catgcaaatt aaaccacatt gattgaatca tgattgcacc ccattcatta
     5821 cttattatag ttttggactt taagccaatc acttaatgat aatcttgttg gatttatttc
     5881 agccattaat tcaaagtcta cttcataacc tttttcttcc aaccattgct tttctgcaac
     5941 accactaaca aattctcctt ctataacagt agatttacct gtcacttcac taaaaattgt
     6001 tgctgcttca cttaatgtaa cttcatcgga accaatctct attgattgat gcgtaaagct
     6061 ttgtggatgt gcaaaaatat acgatgcaat tttagctata tcaatagaag aaatcattgt
     6121 gaattttata ttcggattaa taaattctgg taatgtaata cgttcatctt cgactttagc
     6181 aatgcgtaaa aaattatcca taaagaatga tggtttgata actgttgcat ttatattaga
     6241 ttccattaat ctattttcta tttttgctag tacttcaaag tgtgggccag ttcgatttcg
     6301 attaacccct cccgcagtac tatacacaat atgttgaata ttttcttgct cagctatttc
     6361 aattatcttc ataccttgtc ttaattcttc gctaacatca tctttaacga ttggctgaat
     6421 actgtataag ccatacttcc ctttcatcgc tgattgcaaa ctaacattat cactcagatc
     6481 accttcaacg attgataaat gcggatgtcc tatgtctaag tttacgatta ttcttatttc
     6541 tagttaatgc acttacatac catccatcct ctaacaactg ttttacaact gcattacctt
     6601 gcttccctgt tgcgcctatt actaaaatat ctttcatttt agatccactc ctctttttat
     6661 cttaaaaacc aatagtcctc tttttcacct tttaaatacg tatatggttc tggtgtttgt
     6721 ccatttcctt taatattgtc gtttattaca tctctgacat cccatactaa atgaataaat
     6781 cgttgtaatt gatgactaaa aatgcctagt ggcgctgtca tagctaatac tttttccaca
     6841 tcttctgtag tgacattcag tttttcagca atcatttgaa ccgataaggt agattcttca
     6901 aaatgtgctt gcaactcttc agctgtgatg cgtctttgct ctttatttaa agacattatc
     6961 aacactcttt tcttttatga tatttaatag ttattgcaaa ttcattgcta actttcttaa
     7021 cacgttttat aataaacgga tagttatccg tttgtcaatt ttagagaatt ttaagtaggt
     7081 gtaatcatgc gaaaagatgc aaaagagaat agacaacgaa ttgaagaaat agcgcacaaa
     7141 ctttttgatg aagaaggcgt tgaaaacatt agtatgaatc gtattgctaa agaactaggt
     7201 atcggtatgg ggacattgta tcgacatttc aaagataaaa gcgatttatg ctactacgtc
     7261 atacaaagag accttgatat ttttatcact cattttaagc aaattaaaga tgactatcat
     7321 tctaattatg aggttatgca agtgtcgtta gattatcttt tacaatttaa aatcgataat
     7381 aaggcactac tgcaatgtat tgaagcaggc aacaataaat tacgttttta ccaaagcgca
     7441 ttttatcaag aattatttga tttttattat gacttattta aaagcgatga cgacacttac
     7501 acgaagttca aaacagatat gctgttgcaa tcattatcaa ctagtgtatt tgccttccaa
     7561 atagagcatc gccatatttc aattgaagcg tacagaaatt atttattaaa catttattta
     7621 gatgaggtgg aacgtaatga ctaaaattgt gttaatcaca ggaggcaata aagggttagg
     7681 ctatgcaagt gcagaggcac ttaaagcatt gggttacaaa gtttatatag gttctcggaa
     7741 tgatgtaaga ggacaacaag catcacaaaa attaggtgtt cattatgtac agttagatgt
     7801 tacaagtgac tactcagtca aaaatgctta taacatgatt gctgaaaaag aaggccgtct
     7861 tgatatcctc attaacaatg caggtatatc tggtcaattt tcagcacctt ccaaattgac
     7921 acctcgtgac gttgaagaag tatatcaaac gaatgtattc ggtatcgtac gaatgatgaa
     7981 tacattcgtc cctctcttag aaaaatctga acaacctgtt gtcgtcaacg tatcaagtgg
     8041 tttaggttca tttggaatgg ttacaaaccc cgaaacagct gaatctaaag tgaattcatt
     8101 agcttattgt tcgtctaaat cggcagtaac aatgctaacg ctacaatacg ctaaaggatt
     8161 acctaacatg caaatcaatg ctgctgaccc aggcgctaca aatactgatt tagttggtga
     8221 ttttagtaat aattccaaac acgtttctga aggaatcaag ccaatcattc aattagcaac
     8281 gattggtgca gatggcccaa caggtacatt tattaatggc gatggagaaa tgccttggta
     8341 gatcaatttt atgttacaaa cattgaaatt agaggtatat aaaaaatgct tctgcaatag
     8401 atgcaatata catgcaaaac aatatggagg cgaattaaat gaaaagtatt acgtttgaag
     8461 aacattatgt cattgaagat attcaaaaag aaacgatgaa tgcgatatca gcagatccta
     8521 aaggtgtacc gatgaaagta atgttagaag gccttgaaaa aaagacaggt tttacaaatg
     8581 ccgacgaatt atcacatcat gatgaacgta tccaatttat gaataatcaa gacgttcaaa
     8641 ttcaagtctt atcttatgga aatggatctc cttcaaattt agttggtcag aaagccattg
     8701 aattatgtca aaaagcaaat gatcaattgg caaactatat tgcccaatat cccaatcgct
     8761 ttgtaggctt tgcaacttta cctatcaatg agcctgaagc cgcagcgcga gaattcgaac
     8821 gttgcatcaa tgatttagga ttcaaaggtg cgctcattat gggacgtgca caagatggtt
     8881 ttcttgatca agacaaatat gacattattt tcaaaacagc tgaaaattta gacgtaccga
     8941 tttatctaca tcccgcgcca gttaacagtg acatttatca atcatactat aaaggaaatt
     9001 atcctgaagt aactgcggca acatttgctt gttttggtta tggttggcac attgatgtcg
     9061 gaattcatgc aatacatcta gtattatctg gcatttttga tcgttatcca aagttaaata
     9121 tgattattgg acattggggt gagtttatcc cattcttctt agaacgtatg gatgaagctt
     9181 tattcgctga acatttgaac cactctgtaa gctattactt taaaaatagt ttttatatca
     9241 caccaagtgg catgttaacg aagccacagt ttgatttagt caagaaagaa gtaggtattg
     9301 atagaattct ttatgctgct gattatccat atattgagcc tgaaaaatta ggtgtatttt
     9361 tagatgaact gggtttaaca gatgaagaaa aagagaaaat aagttataca aatggtgcta
     9421 aattattagg tttatcatct aacaattaac aaatggaggt cattgaatat ggaaacttta
     9481 gaattacaag gcgctaaatt acgataccac caagtcggac aaggacccgt gctcatcttt
     9541 attcctggtg caaacggtac cggaaacatt tttctgcctc ttgcagaaca gttaaaagac
     9601 cattttactg ttgtagccgt tgatcgtcgt gattatggag aaagcgagtt aactgaacca
     9661 ctccctgatt ccgcttcaaa ccctgacagt gattatcgtg tcaaacgcga cgcacaggac
     9721 attgccgaac ttgcaaagtc attaagtgat gaacctgtct atatattagg ttcaagttca
     9781 ggttcaatcg ttgcgatgca tgtgttaaaa gattaccctg aagtcgttaa aaagattgca
     9841 tttcacgaac caccaattaa tacattttta ccagacagta cttattggaa agataaaaat
     9901 gatgacattg tgcatcaaat tttaacagaa ggcttagaaa aaggtatgaa aacatttggt
     9961 gaaacattaa atatcgcacc aattgatgca aaaatgatgt ctcaacctgc tgatactgaa
    10021 gaaggacgta tagaacagta caaacgtacg atgttctggt cagaatttga aattagacaa
    10081 tatacgcatt cagatattac tttggatgat ttcacaaaat acagcgataa aatcacccta
    10141 ctaaatggga cagattctag aggctccttc ccacaagatg ttaactttta tataaacaaa
    10201 gaaactggca tacctattgt agatattcca ggcggtcact taggttatat tcaaaaacct
    10261 gaaggctttg ctgatgtatt attaaatatg tggggttaat tgatcgttct ctaataaaac
    10321 agcttggaca gttttcaaat tatatttgat ggctgaccaa gcttattttt gtttacgttt
    10381 aaacgttcaa atcaactcgt tattatacat gaaggcatgt taaccattct cttccactga
    10441 aaacactact gttccaaaca ttgatagtct tcttttgaaa taccctttcc gataagcacc
    10501 aaatattctg gcactttttt tgaaaatgct acaggtgtta attcatattg gccttgtgta
    10561 aattgaatga gatacgtatg tgtggtgtct tcaaatgtca taaacccttt caacctataa
    10621 atatgagacg gaaggcatgc taaacgtttt ataaactctg ctttagtaca tgtgactggt
    10681 gattgcacaa attgatgatt tattgttttg tgtaatgatt catgtttatt gtcagaagtt
    10741 gctgtcattt gtctaactga tattggcaaa gtgacgctgc catgcatacc gacttgtata
    10801 tcggcctctg ggttaataac ttctaaatct ttcaatagtt tgctcgttgt ttcaacatct
    10861 gctgaatcta ttttattaac aaataagaca gaacaatatg ctaattgctc ataaaataag
    10921 ccttggatgt ctttagggaa tgatttaatg tgtttataca tgcttgcgtc tattacacca
    10981 atcatatgtg taattgttgt aaacggagct aaaataggcg ttaaacaagc atctaagaca
    11041 gagactggtt ctgcaatccc actacattca ataaatacaa tgtctggttg ctcttttaaa
    11101 tataattgat gtaactgttc tgatacatct gctttcattg cgcaacagat acacccctct
    11161 gtcagttcac ttaaaggtat gtcttcagat actaaggcac catcaacact cattttccca
    11221 aattcattca tgatgactgc tggtttcagc gattccttta atgattcagc gagcaaatga
    11281 tttaagacag tcgttttacc gccacctaaa aacccaccta taataactat tttcatctgc
    11341 gactctcctc ttatacttca tatgaatagc atattccaag atagatctcc tattgactga
    11401 catgactcta tattcagtta tagttcattt ttttaaataa tcagcattac tattttaaca
    11461 atgatgccta caccaataac taaagacaat gatgtcacca tttgctttcc aattaatttt
    11521 agtgctgact gaccacctaa atgtttcaaa agcatcgtca ttgtgaccga gcacgctgta
    11581 aatgttgaac taaaaaacac aagtagtagt aactgtgttg ctgtcattcc ttgtaataag
    11641 gcgccttgat gcaaattaaa caagagcatg ccgtcttttc gaatcattga aaataaaatc
    11701 cctggtgaca attctgaaga gatgcctaat aacgataata taggtgtaaa tatttgtgat
    11761 aaaacattca aaattggcgt tagtgataaa atactaacaa taagacagat tgtgataaaa
    11821 ataggtagcg cttgaacgat aaacatttgt atattttgcc acatttgtag caacatttga
    11881 cgtatatttg gcatatgtaa ttgcctatca taaggtagcg gaacactaag ttgttgatca
    11941 ttctttttca accatatcct attatgtaag atgccaccta aaagtactaa tattaagtac
    12001 ggcataaata gccatgactt tccagctaca ctaaaaatag ataatgtcgc acctatttga
    12061 taactacaag aactaccaaa gcttattaaa ctcatacact gtgtcttcgt gcaggtatgg
    12121 cattggtgtg ctgcttgtga aatagctgct gcattacatc caaatccttc taatagtggc
    12181 acgatatcat taccttgtaa tccgatccat aacattgacg gttcaattgc ccatatcatc
    12241 catgatttga gtcctgtttg atcaattatt gcagttgata aactaattaa tattacaacc
    12301 ggcaatgccc atacgagcga atatgttcca agtgatagca caccataatc accaaataat
    12361 atatgctgta atatatcgtg attgagcaat gttgtttgag ttaaatattc tatccatgct
    12421 gatacatact gattttgaaa ccaatctgaa aattgataag cgataccaat tggcaatgca
    12481 aacattaaaa atatcaacaa ccaggctaaa aatgtcgcac ttattttagg tcttaaatgt
    12541 cgtcgctcat gttgaaatag tgtcaattgt tctatcacat gttcactcgc tataggcgaa
    12601 gaaactacac tttctgccaa tgaaatatct aatttctgtt ttatgattaa taatcttaat
    12661 ttcgcttttt ctgttgcggt aagtttatcc gcatggctaa taaccaaata atgaggttca
    12721 tgaggtatgg gcgtataatg cccagttctc atatcatatt tcacttctaa catgtgcatg
    12781 acacttcaca acatgaataa tcatctaaat acatttgatt tttttggtaa ttttcaatga
    12841 cctcttgttt agctggtaag ccttcccgct gtgttaaaag atgggcaagt actgcaaatc
    12901 gcgctctgaa tttatagata tagcataatt tggcattatc attttcaact gttgcaccaa
    12961 tcataaaaat attcggataa cgtgtcgatt catcatgtgt tgttaattta atatcttgat
    13021 ttgttgtcac aaataattgt tgaacgattg gattttttgt tgcatcaaag ccagttgcta
    13081 gtattggttc atgaggcgta tgcacacttt gtccgctatc aaaactgata tgatactgtc
    13141 cattgttaaa atcaatatct ttaactgtat aatgtacatt catttcgatg cgagcacctt
    13201 gcttaatgac attacctagt cgctgacgtg tataaggtga caatctaaca ctaggatcag
    13261 catccggatc atttaaaccg gttgtgctag tataaagtgc gatgtcagag ccattttttg
    13321 caagttgata tgcagcatca aagccacttt cattacctcc gataaccaca tattgcccct
    13381 tattaaagtt atcaaagtct tcaatttcac tataatgaat accatattta aatggctttt
    13441 tagggaaatt ataatcacct gttgcgacaa agatataatc cgcgtgatat gtctctgttg
    13501 tcgttgcaat cgtataatat gcatcatctg cagatatatt tgtgacaact gtattttcaa
    13561 agatattcag ctcgtaatgg ttggcaacca cttgtaaata ttcagcatat gtttctccgg
    13621 aaatatgttc ttcattaaat gtaaatgctg gtgaagtatc catggaaatt gcattcatat
    13681 caggcatgcc aaatccatta gacgtaaatg atggcgtgat cgtacgggtc gatttcggcc
    13741 aatgtttaaa tgaatgtcct actgttcctt tttctaaaat aatgacatct gttataccga
    13801 aatcttttaa ggtaatggcc atacctatac ctgcagcacc ggcaccgata atagccactt
    13861 tatgatgttg catattaaca ctccctcaat gatctatttc aagttattgt ttttgactat
    13921 aaatcgtaat aattacgatt tataaagtat agacctatcc ttaagaaatt tcaatattaa
    13981 aagaaaaact tccgttattg tagagaattc atctacattt cggaagttgg tatgtgtctt
    14041 ttttattaaa tttaaggatg ctacggttag ttcaaatgat tatgaatgac atttaatggt
    14101 atcatccagc ctcagtactt ggagataaca ttttggcggt gagacataaa tcatattttt
    14161 acaaaactta tttcgtcgtc tccccaataa agataaagta tttctttatt gtggtgtaac
    14221 ttctccacgt tgtatttcac tatccaaatt ttgtttcata ttttcaaaca actcttgaaa
    14281 tctaacctca tcttgatcag aataaccttt aaaaataact gaatctatat tttcttcgtt
    14341 aaaataataa atttcatctt ccacaacacc aatcggatat ttacatgcag aatagtcaaa
    14401 catatacttt tgattttcaa tttctacaat tggtcctcta ttaataatca ttaacttttg
    14461 cgaaccttct tttaaataaa taatgcttcc tattgtttcc atttattttc ctcctttgtc
    14521 accgtctcaa tttcaacatt ccttcgcatt tccaagtcaa tcatctcttc ttccgcttta
    14581 cgaatgccat gttgaatttg ctgttgctgc tcgtataacg cttccgttga acgtcgtttc
    14641 gcaaaatgaa attcatcgcg gaactgttcc atacgactag ccatattgct actatgataa
    14701 cggtcaccta aatgttgata taattgttct atcgaatgat taagctcatc actttcgtct
    14761 atcaaagctt taacctctcg cttttcatca tataagtcat ccatttgctt atgtttcgct
    14821 gtgatcattt tattgattcc atcaagttta ttcgacatca tttcaccacg gtttaatttt
    14881 taccaatttc attaccaatc attacgtctg tttgattaaa ttcttcgcca actttttgta
    14941 tactatcgct cgcatttctt attgcttcag caacagattg attcttacta atcaaatcgt
    15001 ctatcacact atgagcatta ttatttccac ttactgttgt actttcatct ttatccggtt
    15061 tcgacacatt attacatgtt tcaacggcac ttttaataga tgatgatgca ctattagtct
    15121 tgaaaatatc actttgaact gtactcatta tttacacctc ttcccattta aattatttta
    15181 aaattattag aaactgtatc aatatatgat attcaaacat tcaataatac ttaaatattg
    15241 ttttgtgatt atattcaaaa ttattgtatc aataaatatg tatcattcct agaattttat
    15301 tgaacaaaaa gttataaaat cacttttatt gcaactcaac cttgttttgc attacagaaa
    15361 ttaacaatag cgattaaaat tttattttcc agcaataaaa aagcacaaat atttttattg
    15421 aggcaactga aggccttctc ttcataaaat atctgtgcat attatctatt tatctcaatc
    15481 taacgtctta actaacttgt tgtaccttgt aaaatcctat ccatagaatg cgcaacacta
    15541 tcacggtaca tctcaccaaa tttcaataaa tgcaccatca ataaatataa acgataaaat
    15601 tcaagtctat aggatgctcc ttttgcgagc ggataatgtt tattatacgc atcgtaaaat
    15661 tcgctcgtaa aaccaccaaa taccgttgta ataccgatat caaattctct gtcaccatat
    15721 aatggtgctg gatcaaataa tgcaggacgt ccatctttta aaaacatata attaccgccc
    15781 cataaatcac catgtaataa agacggttta ctttgatgct tttctaattc ctccacaatt
    15841 tgacttcgca ctttgtcata tactttgata tcgttggcat cccatagccc tctatttagc
    15901 aactcttctt tcaaatgatc caaacgttta tcgacaaaaa tggtacgcca atcatcttgc
    15961 caatgattat caaaagaaat atcgccacct tcataaggta atgagaagcc aaatttgcct
    16021 tcttcttgtt gctgactgtg taattgagct acgagttgac ctaattgacg ttgactccct
    16081 gaagctcctt cttctaaata cgtcatcact aaatacacat caccgttaac ttcgccactt
    16141 gcaattactc taggtgccgt gatacctgca cgttcaaatt catttaaacc tgcaatttct
    16201 gcagcataaa atgattcttt acgtccacgt tggacaagta agaaaaatgt atccgtatct
    16261 gtttcgactc gatatgcttc gtttacatca ccaccactca ctggtgaaat ctctttaata
    16321 tcttttaaag gtaaatgctc taaccattgt tcattcatac aaacaccttc caatctactt
    16381 actttatact ataatattgc ctaattagga tgatttaatc ttaaaaacgt tattcaaact
    16441 tgcgtcaatt ttcaaaactt tttagtttta actaggaaat tatgtaggga aatgctagtc
    16501 tattaaaaga atatatttca catatattaa gttgaggata gtaagcctca aatataatga
    16561 aataggagca tactcatgta caaattaatt aaacctttct tattcaaaat cgaacccgaa
    16621 aaagcacacg gactaactat cgatgcatta aaaacgttac aaaagtttcc ggttttattc
    16681 ccagtcgtcg ataaactatt tacttataag aatccaacgt tatcacaaac gatacaaggt
    16741 aatacgtacg acaatccaat tggcttagca gctggtttcg acaaatcttg cgaagtacca
    16801 aaagcattgg aacaccttgg attcggtgct ttagaattag gtggtatcac acctaaacct
    16861 caaccgggta accctcaacc acgcatgttt agattattag aagatgacgc cttgataaat
    16921 cgaatgggct tcaataatat tggtatgaac aaagcactca gtcatttgcg taaaaatgct
    16981 tatcaagtac ctgttggtat caatgttggt gtgaataaaa tgacacctta tgaagcgcgt
    17041 tatcaagatt atataaaagt cattgatacg tttaaacacg acgtttcatt tttcacagtc
    17101 aatatcagtt ctccaaatac tgaaaacctt caaaatttcc atgataaaga tgaattttca
    17161 atgttatgtc aagctttgac aacatttaaa aaacagcacg atgtaacagt gccaatttac
    17221 ttaaaactaa cgtctgatat ggatttcgat ggcttaaaag cactattacc agcgattact
    17281 gagacatttg acggtatcat cttagcaaac acaacgcgac aacgagatgg tttaacttct
    17341 gctaataaag tcgaagaagg cggtttgagt ggtcgtccat tatttgaacg taatttaaaa
    17401 ttgattaagt atgcttatca gcaaacaaat ggtgaatttt taattatagg tacaggcggc
    17461 gtattcagta ctgaagatgc aatcaaaatg atgcgtcacg gtgcgtcact tattcaaatt
    17521 tattcatcac ttgttattga aggcccaggt ttaactaaga aaatgaacaa aggcatcgca
    17581 cgttacttaa aagatcatca ttttgacaat gtcagtgata ttataggact agatgcctaa
    17641 tccaacgtag tcaaataaca ctttaaaact tgctgaatcg tcttattaaa attatgatat
    17701 aactcaagaa gatatattta tcaccatttt tcaaaataat ggcttaaata tcacatatca
    17761 ttaaatatag cgttttataa cggaggcgtt tgttatgact aaagaaacag ataataaaaa
    17821 aacagtagcg acttctgaca acaaagacac acagcaacaa aatcaaactg aagaagatga
    17881 acgtcaaggt ttgaatggtt atcgtaaaac agatcttgat ttagaaattg agcaagagct
    17941 acgtgaaatg atgaaaacag gagaaaatga aacgaaaaac gactacaaaa agtttaaagt
    18001 gttttcactt atttcaacac ttgtcattgt cattttagca attataagat ttgttcataa
    18061 aatgatttaa ttagagtgag acattgtttt atgtctcagg atccagttat tcagtatagc
    18121 tacaatattt acgattatat aaataacccg agattttagt atgattcatt tcactaaaat
    18181 ctcgggtttc tatttgataa tttttaatgg gatatggcat gtatacgagc ctgcctttta
    18241 tctcatttcc aatgattaat ctggatattg ttctaaaaat gctttcgctt ctttattaac
    18301 tgttttaaaa tcaatacctt gttgcatcgc tgcaaagaca catccacaat aacactgcct
    18361 aaagatatta tagtcattac acatttctat ggaacgctca taacctttac ttttcttaaa
    18421 atcacttggc aaatagttca catcgtatat tttttggaca tccataccaa gttcattgat
    18481 taattgtgcg ttctttttag gtgataacgt gattgcactg ccaaaataat catagccatg
    18541 ttctacagct gctttcgcta caatatccaa acgcatttca aaacaagcgg tacaacgtaa
    18601 gccgccttct ttttcatcag ctaattcttt atccttcacc attttcacaa acttatgcgg
    18661 ttcataaggt gcttcaatat acttcacatt cgcaccagtc ttgcgattaa aatcttccac
    18721 aaattgttct tgcactttag cacgtcgtaa atattcattt ttcggatgaa tatttgaatt
    18781 cgcgaaataa attgcaatgt ctgcatattg tgttaaaaac tctaatgtat atgtactaca
    18841 aggtgcacaa caactatgca ataagatttt aggtctgatt gcttctcttt cccactgccc
    18901 gattaatttc ttcaacacct tgtcataatt aattttttga tttttcattt tactgataat
    18961 aggttcagca ttaatcatga tttaacctcc cacatttaat cattaacttc tattatatat
    19021 gattcatatt aaatgtcagt caaaaaagtt agaaattcat tttaatgcat ttatatttcg
    19081 aaagtccctt atgtatctaa aagcattttt taaacttgaa ttttaaaaca ctaaacaaca
    19141 catacgtctc tgtgtcattt tcattttttg tatgtcatat atatgtttac ttcatttaaa
    19201 tcaatttcat cttacaattt atcgtgtatt ttacaaaaga ttgacttcaa ttcatcgtaa
    19261 aagttatact tttgccattt tttaatgtaa catggtgtta gtaataaaaa tactacattg
    19321 aggtgtttta catgacagca ttattccctt atatcgcttt tgaaaattca aaagaagccc
    19381 ttgcatatta cgaagaagta tttggtgcaa ctgacgttaa acgtttagaa gttggcgaag
    19441 aacaagcgtc acattttggt atgactaagg aagaagcgca agaagcaact atgcatgctg
    19501 aatttgaagt gcttggcgta aaagtgctat gttctgattc ttttggtcgc gctgacaaaa
    19561 ttaataatgg catatcatta ttaattgatt atgatgttaa caataaggaa gatgctgata
    19621 aagttgaagc attctatgag aaaattaaag atcattcttc aattgaaata gaattaccgt
    19681 ttgctgacca attctggggt ggcaaaatgg gcgtctttac cgataaatac ggtgttcgtt
    19741 ggatgttaca tggtcaagac tatactgcaa tccaacaata atgttattga taaagatatc
    19801 atttaataag cttatgttat ttagcaaggc aagccactct cgattcatgg cttgccttat
    19861 tttattgtat atttttaatt atcgttgctg gcccttgatt gacaatcgta ttaaaatgca
    19921 ttattaagtc aactttgtct atacggtttg gatcttctac ccaatgtctt atcaaagaca
    19981 atcccgcacc tgaaacataa ctcatgaaat aagaaaatgg tataccatta atttgatcat
    20041 tttcattttt ataacgttgt aaatgacaca taattaagtc atatagtttt tcgtgtatat
    20101 ttgaatcttt acccatacga aacatcaatt gataaaatgc gatgtctttt tctatcatat
    20161 ctattaaaac agtcataatt tgatgtatgt tatccgtgga taacttaact gctccattta
    20221 acttctcatc atgaatgaag tctcttattt cctccaactg ctggtcctct aatttttcaa
    20281 gcaaatcata tttatcataa taatgcgtat aaaatgtact acggttaaca tcagctaaat
    20341 ctgcaatttg ttgcacagta atctcttcta attggtgttg atgtaaaagt tcaataaatg
    20401 catttctcat tgcaacttgt gattttctaa tacgtcgatc tatagtcatt tatatcaagt
    20461 cctccccaat gattataaac aattataaac gttatgttca ttatcccaca aatctccaac
    20521 attgatgatt ggcacacaat gtttacctgt ttaatatagg tgatacaaac aaacagaaaa
    20581 aggtgataac aatgaaccaa catttactag gaaatccaaa attaactgta actcatgtca
    20641 atgaagtgaa aaccggtatt aaccacatcg ttgtcgacag tgttcaatat ggaaatcaag
    20701 aaatgattat ggaaaaagat gtcactgtgg aaatgcgcga tggcgaaaaa ttatatatta
    20761 atattttcag accaaataaa gatggcaaat tccctgtagt tatgtctgca gatacttacg
    20821 gtaaagataa taagcctaaa atcaccaata tgggtgccct ttggccaaca ttaggcgcca
    20881 ttccgacatc tagttttaca cctgaagaat caccagaccc aggattttgg gtaccaaacg
    20941 attatgttgt agttaaagtt gcattacgcg gtagtgacaa atccaaaggc gtcttatctc
    21001 catggtcaaa aagagaagcg gaagattatt acgaagtgat tgaatgggca gcaaaacagc
    21061 catggagtaa tggaaatatc ggaacaaatg gtgtttctta tcttgcggtg actcaatggt
    21121 gggtcgcatc attaaatcca ccacatttaa aagcaatgat tccttgggaa ggattaaatg
    21181 atatgtatag agaagtagcc tttcacgggg gtattcctga tactggcttt tatcgtttct
    21241 ggatacaagg tatttttgcg agatggacag ataatccaaa tatcgaagac atgattcaag
    21301 cacaacaaga acaccctttg ttcgatgatt tttggaaaca gcgtcaagtg ccattatcac
    21361 aaattaaaac acctctacta acatgtgcta gttggtctac acaaggtttg cacaaccgtg
    21421 gctcttttga aggatttaaa caagctgcat cagaagaaaa atggctatat gtgcatggac
    21481 gtaaagagtg ggaaagttac tacgctagag aaaatctcga acgccaaaaa tcattctttg
    21541 atttttacct taaagaagaa aataacgatt ggaaagatac gcctcatgtc atatatgaag
    21601 ttagagatca attttataaa ggcgaattca aatcagcgtc agcgttccct ttacctaaca
    21661 cagaatatac accattgtat ttaaatgctg aaaatcacac attgaatcat gcaaagatta
    21721 gtagcgcgta tgtcgcacaa tatgactctg aagataaacg acaagatgta agttttaaat
    21781 atacgtttga caaagatact gagttagttg gaaacatgaa cttaaaacta tgggtaagca
    21841 ctacagactc agacgatatg gatttatttg caggtattaa aaagttagat cgtcgtggta
    21901 atgaagttaa cttccctgat tttaatcata ttgaaaatgg tcaagtagct actggttggt
    21961 tacgcgtatc acatcgtgaa ttagatcaag aaaaatccac aatcgcgcaa ccttggcata
    22021 aacatgaaac agaattaaag ttgtcacaag atgagattgt acctgttgaa atcgaattgt
    22081 taccttcagg cacgctattt aaacaaggcg aaacattgga agttgttgta aaaggtagtg
    22141 aaattgtaat tggtaatagt acccctggca tgaaaacacg ttatgaacat gaagaaacca
    22201 taaataacgg catgcacatg atttatactg gtggtaaata tgattcacaa ttaatcattc
    22261 ctatcgttaa ttgacatgca ggaattacgg tcgattttga ttaaaagtga catagtgata
    22321 gaagagaagg ccacacgctt ggaatcttaa accaaggtgt ggcccttttt attattgatg
    22381 gctatttaat tttataaatc ttgtcactaa acttactaca agcaatatta aaccaataaa
    22441 taaagccatt attgctgtat tactttcgtt gtttactcct gtttttggca attctttcaa
    22501 ttgttgctta tgtactgact tgctcttgct atccaaatga atactttgtt gtttagccat
    22561 ttgtttatta ctttttgtgt gaatatcatt tgctttaata cttggattta cagcactatt
    22621 attcgatgtt ccatttgcgt ttttcaactg attaataaca tttggatctt tcgtgccatc
    22681 tattaaatta tctggatgtt ttagtaaatg tctgcagtcg gatactattt tagaatcttt
    22741 tgcctttacc gcattattcg gcacctcatt atcatctttc aactgcttga taacatctgg
    22801 atctttcgtg ccatctatta aattatcaag ttttttcaca cgtgctatgt cactaactgg
    22861 ccctggataa tttgcatccg gttgtctaac tggtattaaa ttgctgtttt cattcacacc
    22921 ttcaggtttg ttattttcgg cgttcgcatt atccgtaaaa atcaacactg ataaaattgc
    22981 aacaaataaa cttataactt ttttcatctt attcatcctt tcctatttta ctaccttaat
    23041 atcattgcta ttaaagtaac ccatccatct atttttttcg tcgtataaag agtaatattt
    23101 atacccattt ggaatattgt aatagagttt agctgtgtac gctttatctt ttttaattaa
    23161 tttattatca cccttttttg taatttcaat gtttttccaa tatatatact tacttttaat
    23221 tactttcact ttcttattat atggatgact tctatactta cctatccaat aatttatgta
    23281 atctaatttt aaatcattta ttctcacgcc tgaattatga ttatatctgc cgtatgcatg
    23341 tactccgatg atattgtttt ttgaatctct tatcgcacta ccactttgtc ctgaaaatgt
    23401 gtctaccgta taatacattt cattctcatc aaaactttct aatttccctt tacctttata
    23461 ttgtctgttg tctgacttgt ctcctggaaa tccagaaatt tcaatatttt cacctttgct
    23521 aatgtgtgtg ttcaaagtta agtaacctgt tttatttcct aaattactat ttaacttaat
    23581 aattgctata tcttcttttg taggagcatt attaatccaa gttttagata caaaaaactt
    23641 atgcgaaaag gcctttccta ttgtatatgt ttgcccattt actccagcat agacatcaat
    23701 ctttttagcc caacccttat cgtctttaga atagacatta tgagccgctg tcaatacaat
    23761 gtccttacca atcatagtac cggtgccttt atatacacgg ttatttgaaa acgtcatatt
    23821 taataataca gtctgcttat aaggtctttg taaaatatta ttaacaaccg ttctttgatc
    23881 tttaccgaaa actcgctcag ttaagggcgt actataatca cctacttcat aaagctcatt
    23941 ttgattacgt tcaattgttc ctgattcaaa attattatca ctaccatttt ctgaattgac
    24001 gataggatta ttatcagcta agacaaaact ttcaccataa taactagaaa caattactac
    24061 taacaaaatc aaaaatgata ttttaaaaat ttttgtcata ggcattcctc ct
//
LOCUS       DAHHSC010000020        21965 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.020, whole genome shotgun sequence.
ACCESSION   DAHHSC010000020 DAHHSC010000000
VERSION     DAHHSC010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 21965)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21965)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21965
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.020"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            250..2856
                     /gene="yfhO"
                     /locus_tag="NBI57_000633"
     CDS             250..2856
                     /gene="yfhO"
                     /locus_tag="NBI57_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoteichoic acid-specific glycosyltransferase
                     YfhO"
                     /protein_id="HCD1821600.1"
                     /translation="MLKKWLNSNVKQFFVITFISVILTLILFSTHIYDYIVNGTVFSG
                     AGDGFRQMMPFQMYLYEHLSSFSSLYDASFGLGGDYMKGLSYYYSLSPLMWLNFLFIK
                     IGETVGIFNPTTIHFWPTNQLIMAMIRAIITFVVTFYLFKILHFKRSTNMIATILYGM
                     STVVIYFNFTWSFYGNLLYLLPLSILGLERYFQQRKIGIFIVAIALTLFSNFYFSYYQ
                     AIIIGCYYLYRLIFTYKYDIVSRTQKFICVISATVLSVLSSVFGLFTGISAFLENDRK
                     QNPNVDIPFLTPLDYHYFFFSDGFYITISILTIVALLSFKLYRFYFYRLFAIVTWILF
                     IGSLSQYFDSAFNGFSFPERRWVYILALSSSALCGLFIQHLSTLNMKYYLIRTIPVSI
                     IALLYVLLSPTHPLALIVGIILLMVLAVILKFSLWRYKKLTVAILVLIVMIQQIVILD
                     NNKNMAIKPYQQSLSTLKQHDYHSNYVNQLIKKINQNATGPFNRIDYMSDYALNSPFI
                     YHYNGISLYSSIFNGDILKYYDKTLQINMPIDKNSTYRLLGNRQNLLSLWNVNDRIRV
                     NHDDNLPYRFKINSEHKDNKVRWIHSKNTIHYPSAHITNKVFSNKELKSPLDKEQAML
                     QGIVSNNTKDVNTHFKANKNLLSDSTIKLNSSAWQSPTKHLLQVKQNNGGLTVQLPKS
                     VSNQFKDLYFEMDLELLSPDKAHDVKVNEYTQERNKLTYKYRRFVTPVTIRIKASDRI
                     RLSLPKGKYRVNLKGIYGEDYTTLKDASNSLEAVKVSKTKQGYTITKNKNSSGYIVLP
                     TAYNQGMKATSGDQSLKVAQVNGVMTGIKAPKNITKIQLSYTPPYYYLLITITIFGII
                     CSIIFTRWARQK"
     gene            complement(3100..3450)
                     /gene="rplS"
                     /locus_tag="NBI57_000634"
     CDS             complement(3100..3450)
                     /gene="rplS"
                     /locus_tag="NBI57_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011303276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HCD1821601.1"
                     /translation="MTNHKLIEAVTKSQLRTDLPSFRPGDTLRVHVRIIEGTRERIQV
                     FEGIVIKRRGGGVSETFTVRKISSGVGVERTFPLHTPKIEKIEVKRRGKVRRAKLYYL
                     RSLRGKAARIQEIR"
     gene            complement(3553..4290)
                     /gene="trmD"
                     /locus_tag="NBI57_000635"
     CDS             complement(3553..4290)
                     /gene="trmD"
                     /locus_tag="NBI57_000635"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HCD1821602.1"
                     /translation="MKIDYLTLFPEMFDGVLNHSIMKRAQENNKLQINTVNFRDYAIN
                     KHNQVDDYPYGGGQGMVLKPEPVFNAMEDLDVTEQTRVILMCPQGEPFSHQKAVELSK
                     ADHIVFICGHYEGYDERIRTHLVTDEISMGDYVLTGGELPAMTMTDAIVRLIPGVLGN
                     EQSHQDDSFSDGLLEFPQYTRPREFKGLTVPDVLLSGNHANIDAWRHEQKLIRTYNKR
                     PDLIEKYPLTNEDKQILERYKIGLKKG"
     gene            complement(4290..4793)
                     /gene="rimM"
                     /locus_tag="NBI57_000636"
     CDS             complement(4290..4793)
                     /gene="rimM"
                     /locus_tag="NBI57_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HCD1821603.1"
                     /translation="MRVEVGQIVNTHGIKGEIKVKSNSDFTDVRFQPGQVLTVVHNNN
                     DLEYTVKSHRVHKGLHMLTFEGINNINDIEHLKGSSIYQERDHEDIVLEENEFYYSDI
                     IGCTVFDDQETPIGRVINIFETGANDVWVIKGSKEYLIPYIADVVKEVDVENKKIIIT
                     PMEGLLD"
     gene            complement(4981..5256)
                     /gene="rpsP"
                     /locus_tag="NBI57_000637"
     CDS             complement(4981..5256)
                     /gene="rpsP"
                     /locus_tag="NBI57_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HCD1821604.1"
                     /translation="MAVKIRLTRLGSKRNPFYRIVVADARSPRDGRIIEQIGTYNPTS
                     ANAPEIKVDEALALKWLNDGAKPTDTVHNILSKEGIMKKFDEQKKAK"
     gene            complement(5691..7058)
                     /gene="ffh"
                     /locus_tag="NBI57_000638"
     CDS             complement(5691..7058)
                     /gene="ffh"
                     /locus_tag="NBI57_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002457378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HCD1821605.1"
                     /translation="MAFEGLSERLQATMQKMRGKGKLTEADIKIMMREVRLALFEADV
                     NFKVVKEFIKTVSERALGSDVMQSLTPGQQVIKIVQDELTKLMGGENTSINMSNKPPT
                     VVMMVGLQGAGKTTTAGKLALLMRKKYNKKPMLVAADIYRPAAINQLQTVGKQIDIPV
                     YSEGDQVKPQQIVTNALKHAKEEHLDFVIIDTAGRLHIDEALMNELKEVKEIAKPNEI
                     MLVVDSMTGQDAVNVAESFDDQLDVTGVTLTKLDGDTRGGAALSIRSVTQKPIKFVGM
                     SEKLDGLELFHPERMASRILGMGDVLSLIEKAQQDVDQEKAKDLEKKMRESSFTLDDF
                     LEQLDQVKNLGPLDDIMKMIPGMNKMKGLDKLNMSEKQIDHIKAIIQSMTPAERNNPD
                     TLNVSRKKRIAKGSGRSLQEVNRLMKQFNDMKKMMKQFTGGGKGKKGKRNQMQNMLKG
                     MNLPF"
     gene            complement(7084..7416)
                     /locus_tag="NBI57_000639"
     CDS             complement(7084..7416)
                     /locus_tag="NBI57_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HCD1821606.1"
                     /translation="MGQNDLVKTLRMNYLFDFYQSLLTNKQRNYLELFYLEDYSLSEI
                     ADTFNVSRQAVYDNIRRTGDLVEDYEKKLELYQKFEQRREIYDEMKQHLSNPEQIQRY
                     IQQLEDLE"
     gene            complement(7403..8653)
                     /gene="ftsY"
                     /locus_tag="NBI57_000640"
     CDS             complement(7403..8653)
                     /gene="ftsY"
                     /locus_tag="NBI57_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HCD1821607.1"
                     /translation="MSFFKRLKDKFATNKENEEVKSLREEQGQDKLEDTHSEGSTQDV
                     NDLAENAEVKKKPRKLSEADFDDDGLISIEDFEEIEAQKMGAKFKAGLEKSRQNFQEQ
                     LNNLIARYRKVDEDFFEALEEMLITADVGFNTVMTLTEELRMEAQRRNIQDTEDLREV
                     IVEKIVEIYHQEDDNSEAMNIEDGRLNVILMVGVNGVGKTTTIGKLAYRYKMEGKKVM
                     LAAGDTFRAGAIDQLKVWGERVGVDVISQSEGSDPAAVMYDAINAAKNKGVDILICDT
                     AGRLQNKTNLMQELEKVKRVINRAVPDAPHEALLCLDATTGQNALSQARNFKEVTNVT
                     GIVLTKLDGTAKGGIVLAIRNELHIPVKYVGLGEQLDDLQPFNPESYVYGLFADMIEQ
                     NDEITTVENDQIVTEEKDDNHGAK"
     gene            complement(8653..12219)
                     /gene="smc"
                     /locus_tag="NBI57_000641"
     CDS             complement(8653..12219)
                     /gene="smc"
                     /locus_tag="NBI57_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HCD1821608.1"
                     /translation="MVYLKSIDAIGFKSFADQTNVQFDKGVTAIVGPNGSGKSNITDA
                     IKWVLGEQSAKSLRGSKMEDIIFSGAEHRKAQNYAEVQLRLDNHSKKLSVDENEVIVT
                     RRLYRSGESEYYINNDRARLKDIADLFLDSGLGKEAYSIISQGRVDEILNAKPIDRRQ
                     IIEESAGVLKYKKRKAESLNKLDQTEDNLTRVEDILYDLEGRVEPLKEEAAIAKEYKT
                     LSHQMKHSDIVVTVHDIDQYTNDNRQLDQRLNDLQGQQANKEADKQRLSQQIQQYKGK
                     RHQLDNDVESLNYQLVKATEAFEKYTGQLNVLEERKKNQSETNARYEEEQENLMELLE
                     NISNEISEAQDTYKSLKSKQKELNAVIRELEEQLYVSDEAHDEKLEEIKNEYYTLMSE
                     QSDVNNDIRFLKHTIEENEAKKSRLDSRLVEVFEQLKDIQGQIKTTKKEYQQTNKELS
                     AVDKEIKNIEKDLTDTKKAQNEYEEKLYQAYRYTEKMKTRIDSLATQEEEYTYFFNGV
                     KHILKAKNKELKGIHGAVAEIIDVPSKLTQAIETALGASLQHVIVDSEKDGRQAIQFL
                     KERNLGRATFLPLNVIQSRVVATDIKSIAKEANGFISIASEAVKVAPEYQNIIGNLLG
                     NTIIVDHLKHANELARAIKYRTRIVTLEGDIVNPGGSMTGGGARKSKSILSQKDELTT
                     MRHQLEDYLRQTESFEQQFKELKIKSDQLSELYFEKSQKHNTLKEQVHHFEMELDRLT
                     TQETQIKNDHEEFEFEKNDGYTSDKSRQTLSEKETHLESIKASLKRLEDEIERYTKLS
                     KEGKESVTKTQQTLHQKQSDLAVVKERIKTQQQTIDRLNNQSQQTKHQLKDVKEKIAF
                     FNSDEVMGEQAFQNIKDQINGQQETRTRLSDELDKLKQQRIELNEQIDAQEATLQVCH
                     QDILAIENHYQDIKAEQSKLDVLIHHAIDHLNDEYQLTVERAKSEYTSDESIDALRKK
                     VKLMKMSIDELGPVNLNAIEQFEELNERYTFLSEQRTDLRKAKETLEQIISEMDQEVT
                     ERFKETFHAIQGHFTAVFKQLFGGGDAELQLTEADYLTAGIDIVVQPPGKKLQHLSLL
                     SGGERALTAIALLFAILKVRSAPFVILDEVEAALDEANVIRYAKYLNELSDETQFIVI
                     THRKGTMEFADRLYGVTMQESGVTKLVSVNLNTIDDVLKEEQ"
     gene            complement(12366..13097)
                     /locus_tag="NBI57_000642"
     CDS             complement(12366..13097)
                     /locus_tag="NBI57_000642"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HCD1821609.1"
                     /translation="MSKQKKSEIVNRFRKRFDTKMTELGFTYQNIDLYQQAFSHSSFI
                     NDFNMNRLDHNERLEFLGDAVLELTVSRYLFDKHPNLPEGNLTKMRATIVCEPSLVIF
                     ANKIGLNEMILLGKGEEKTGGRTRPSLISDAFEAFIGALYLDQGLDIVWKFAEKVIFP
                     HVEQNELLGVVDFKTQFQEYVHQQNKGDVTYNLIKEEGPAHHRLFTSEVILQGEAIAE
                     GKGKTKKESEQRAAESAYKQLKQIK"
     gene            complement(13213..13446)
                     /locus_tag="NBI57_000643"
     CDS             complement(13213..13446)
                     /locus_tag="NBI57_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002510960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HCD1821610.1"
                     /translation="MENFDKVKDIIVDRLGVDADKVTEDASFKDDLGADSLDIAELVM
                     ELEDEFGTEIPDEEAEKINTVGDAVKFINSLEK"
     gene            complement(13881..14615)
                     /gene="fabG"
                     /locus_tag="NBI57_000644"
     CDS             complement(13881..14615)
                     /gene="fabG"
                     /locus_tag="NBI57_000644"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HCD1821611.1"
                     /translation="MTKSALVTGASRGIGRSIALQLAEEGYNVAVNYAGSKEKAEAVV
                     EEIKAKGVDSFAIQANVADADEVKAMIKEVVSQFGSLDVLVNNAGITRDNLLMRMKEQ
                     EWDDVIDTNLKGVFNCIQKATPQMLRQRSGAIINLSSVVGAVGNPGQANYVATKAGVI
                     GLTKSAARELASRGITVNAVAPGFIVSDMTDALSDELKEQMLTQIPLARFGQDTDIAN
                     TVAFLASDKAKYITGQTIHVNGGMYM"
     gene            complement(14608..15534)
                     /gene="fabD"
                     /locus_tag="NBI57_000645"
     CDS             complement(14608..15534)
                     /gene="fabD"
                     /locus_tag="NBI57_000645"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HCD1821612.1"
                     /translation="MSKTAIIFPGQGAQKVGMAQDLFNNNDQATEILTSAAKTLDFDI
                     LETMFTDEEGKLGETENTQPALLTHSSALLAALKNLNPDFTMGHSLGEYSSLVAADVL
                     SFEDAVKIVRKRGQLMAQAFPTGVGSMAAVLGLDFDKVDEICKSLSSDDKIIEPANIN
                     CPGQIVVSGHKALIDELVEKGKSLGAKRVMPLAVSGPFHSSLMKVIEEDFSSYINQFE
                     WRDAKFPVVQNVNAQGETDKEVIKSNMVKQLYSPVQFINSTEWLIDQGVDHFIEIGPG
                     KVLSGLIKKINRDVKLTSIQTLEDVKGWNEND"
     gene            complement(15527..16513)
                     /gene="plsX"
                     /locus_tag="NBI57_000646"
     CDS             complement(15527..16513)
                     /gene="plsX"
                     /locus_tag="NBI57_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HCD1821613.1"
                     /translation="MVKLAIDMMGGDNAPDIVLEAVQKAVEDFKNLEIMLFGDEKKYN
                     LNHERIEFRHCSEKIEMEDEPVRAIKRKKDSSMVKMAEAVKSGEADGCVSAGNTGALM
                     SVGLFIVGRIKGVARPALVVTLPTIDGKGFVFLDVGANADAKPEHLLQYAQLGDIYAQ
                     KIRGIDNPKISLLNIGTEPAKGNSLTKKSFELLNQDHSLNFVGNIEAKTLMDGDTDVV
                     VTDGYTGNMVLKNLEGTAKSIGKMLKDTIMSSTKNKLAGAILKKDLAEFAKKMDYSEY
                     GGSVLLGLEGTVVKAHGSSNAKAFYSAIRQAKIAGEQNIVQTMKETVGESNE"
     gene            complement(16518..17090)
                     /gene="fapR"
                     /locus_tag="NBI57_000647"
     CDS             complement(16518..17090)
                     /gene="fapR"
                     /locus_tag="NBI57_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription factor FapR"
                     /protein_id="HCD1821614.1"
                     /translation="MRGETLKLKKDKRREAIRQQIDSNPFITDHELSDLFQVSIQTIR
                     LDRTYLNIPELRKRIKLVAEKNYDQISSIEEQEFIGDLIQVNPNVKAQSILDITSDSV
                     FHKTGIARGHVLFAQANSLCVALIKQPTVLTHESSIQFIEKVKLNDTVRAEARVVNQT
                     AKHYYVEVKSYVKHTLVFKGNFKMFYDKRG"
     gene            complement(17293..19353)
                     /gene="recG"
                     /locus_tag="NBI57_000648"
     CDS             complement(17293..19353)
                     /gene="recG"
                     /locus_tag="NBI57_000648"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HCD1821615.1"
                     /translation="MAKVNLIESPYSLLQLKGIGPKKIEVLQQLNIHTVEDLVLYLPT
                     RYEDNTVIDLNQAEDQSNVTIEGQVYTAPVVAFFGRNKSKLTVHLMVNNIAVKCIFFN
                     QPYLKKKIELNQTITVKGKWNRVKQEITGNRVFFNSQGTQTQENADVQLEPVYRIKEG
                     IKQKQIRDQIRQALNDVTIHEWLTDELREKYKLETLDFTLNTLHHPKSKEDLLRARRT
                     YAFTELFLFELRMQWLNRLEKSSDEAIEIDYDLDQVKSFIDRLPFELTEAQKSSVNEI
                     FRDLKAPIRMHRLLQGDVGSGKTVVAAICMYALKTAGYQSALMVPTEILAEQHAESLM
                     ALFGDSMNVALLTGSVKGKKRKILLEQLENGTIDCLIGTHALIQDDVIFHNVGLVITD
                     EQHRFGVNQRQLLREKGAMTNVLFMTATPIPRTLAISVFGEMDVSSIKQLPKGRKPII
                     TTWAKHEQYDKVLMQMTSELKKGRQAYVICPLIESSEHLEDVQNVVALYESLQQYYGV
                     SRVGLLHGKLSADEKDEVMQKFSNHEIDVLVSTTVVEVGVNVPNATFMMIYDADRFGL
                     STLHQLRGRVGRSDQQSYCVLIASPKTETGIERMTIMTQTTDGFELSERDLEMRGPGD
                     FFGVKQSGLPDFLVANLVEDYRMLEVARDEAAELIQSGVFFENTYQHLRHFVEENLLH
                     RSFD"
     gene            complement(19543..21189)
                     /gene="fakA"
                     /locus_tag="NBI57_000649"
     CDS             complement(19543..21189)
                     /gene="fakA"
                     /locus_tag="NBI57_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid kinase catalytic subunit FakA"
                     /protein_id="HCD1821616.1"
                     /translation="MISKINGKLFADMIIQGAQNLSNNADLVDSLNVYPVPDGDTGTN
                     MNLTMTSGREEVENNLSKNIGELGKTFSKGLLMGARGNSGVILSQLFRGFCKNIESES
                     EINSKLLAESFQAGVETAYKAVMKPVEGTILTVAKDAAQAAIEKANNTEDCIELMEYI
                     IVKANESLENTPNLLAVLKEVGVVDSGGKGLLCVYEGFLKALKGEKVEAKVAKIDKDE
                     FVHDEHDFHGVINTEDIIYGYCTEMMVRFGKNKKAFDEQEFRQDMSQFGDSLLVINDE
                     EIVKVHVHTEYPGKVFNYGQQYGELIKLKVENMREQHREVIRKEQHTAKPKMETVETA
                     IITISMGEGISEIFKSMGATHIISGGQTMNPSTEDIVKVIEQSKCKRAIILPNNKNIL
                     MASEQAASIVDAEAVVIPTKSIPQGISALFQYDVDATLEENKAQMADSVNNVKSGSLT
                     YAVRDTKIDGVEIKKDAFMGLIEDKIVSSQSDQLTTVTELLNEMLAEDSEILTVIIGQ
                     DAEQAVTDNMINWIEEQYPDVEVEVHEGGQPIYQYFFSVE"
     gene            complement(21204..21578)
                     /locus_tag="NBI57_000650"
     CDS             complement(21204..21578)
                     /locus_tag="NBI57_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HCD1821617.1"
                     /translation="MTLEISNDYGKIDISNEVIASVVGGKAVECYGIVGMASRQQVRD
                     GIAEILGHENYAKGIKVTENNGVVDIDMYIIVSYGVKISEVANNVQSTVKYTLEKSLN
                     VSVNSINIYVQGVRVNNTGKKA"
BASE COUNT         6621 a         4245 c         2926 g         8173 t
ORIGIN      
        1 agttttctac aattaaaaat catcatttgt taaatctaag ttacttatta agtgacattc
       61 atctttatca gtatgacatt tttattattc aatatatttg tttaatgata aactgaaaat
      121 gatccaaagt ttaagtaact atgataaggt atctgttcca cagtcatctt cgtctttacc
      181 tatcaagaac ctcattaact ttagtttcag ttaaacttaa ttttaaattt gaaagttgag
      241 gataatgttg tgttaaaaaa gtggctaaat tcaaacgtca aacaattctt tgttataact
      301 ttcattagtg taatattaac gcttatttta ttttctactc atatctatga ttatattgtg
      361 aatggtactg tttttagtgg ggctggagat ggattccgtc aaatgatgcc atttcaaatg
      421 tatttgtatg aacatctaag tagtttttct agtttatatg atgcatcgtt tggattaggt
      481 ggcgattata tgaaaggact atcatattat tattcgctgt cacctttaat gtggctaaat
      541 tttctattca ttaaaatagg agaaacggtt ggtatattta atccgacaac aatacatttt
      601 tggccgacaa accaacttat tatggctatg atacgagcta tcataacatt tgtcgtgacc
      661 ttctacttat ttaaaatatt acactttaaa cgctcaacaa atatgatcgc tacgatttta
      721 tacggcatgt caactgtcgt tatatacttt aattttactt ggtcatttta tggaaattta
      781 ttatatttat tgccattatc gattcttggt ttggaaagat attttcaaca acgcaaaatc
      841 ggtattttca ttgttgcgat agccttaaca ctatttagca atttttattt cagttattat
      901 caagctatta ttataggttg ctactattta tatcgactca ttttcactta caaatatgac
      961 attgtctcta gaacacaaaa atttatttgc gtcatatctg ctacagtttt gagtgtgtta
     1021 tcaagtgtat ttggtttatt cactggcatt tctgcgtttt tggaaaatga cagaaagcaa
     1081 aatcccaatg ttgatatacc gtttttgaca ccacttgatt atcattattt tttctttagc
     1141 gatggatttt atattacgat ttcaattctt actatcgttg cattattgtc attcaaactg
     1201 tatcgttttt acttttatag acttttcgca atagtaacat ggatattatt tattggttca
     1261 ttatcacagt atttcgacag tgcttttaat ggtttttcat ttccagaaag gcgttgggtg
     1321 tatatcttag cactatcatc aagcgctctt tgcggattgt ttattcaaca tttatcaaca
     1381 ttaaatatga aatattattt aatcagaaca ataccagtaa gtatcatcgc attactttat
     1441 gtattactat caccgacaca cccacttgca cttatagtag gtattatcct gctaatggtg
     1501 cttgccgtta ttttaaaatt tagtttatgg cgttataaaa aattaaccgt tgcaatatta
     1561 gtattaatcg ttatgattca acaaatcgtc attttagata acaacaaaaa tatggcaatc
     1621 aaaccttatc aacaatcatt atcaacgttg aaacaacatg attaccatag taactatgta
     1681 aaccagctta taaaaaagat aaatcaaaat gcaacaggcc catttaatcg cattgattat
     1741 atgtcagact atgcattaaa ttcaccattt atatatcatt ataatggcat ttcattatat
     1801 tctagtattt ttaatggaga cattttaaaa tattatgaca agacactcca aattaatatg
     1861 ccaattgata aaaacagcac ttatagatta cttggcaatc gtcaaaattt actatcactt
     1921 tggaatgtta atgatcgaat tagagtgaat catgatgaca acttaccata tagatttaaa
     1981 attaactctg aacacaaaga caataaagtt cgttggattc attctaaaaa taccatccat
     2041 tatccaagtg cacatattac aaataaggtc ttttccaata aagaattaaa atctccatta
     2101 gataaagaac aagcaatgtt gcaagggatt gtttctaaca atactaaaga tgttaataca
     2161 cattttaaag ccaataaaaa tttactatca gattcaacaa ttaaattaaa tagttcagcc
     2221 tggcaatctc ctacaaaaca tttattacaa gttaaacaaa ataatggtgg tctaactgta
     2281 cagttgccaa aatcagtttc taatcaattt aaagatttgt attttgaaat ggatttagaa
     2341 ttactttcgc cggataaagc tcatgatgtt aaagtgaatg aatatacaca agaaagaaat
     2401 aaactcactt ataaatatcg acgctttgta acacccgtaa cgatacgcat taaagcttca
     2461 gatagaatta gattatcatt gcctaaaggt aagtatcgag taaatttaaa agggatatac
     2521 ggcgaagatt ataccacgct taaagacgct tcaaattcat tagaggctgt caaagttagt
     2581 aagacaaagc aaggttatac tattactaaa aataaaaatt catctgggta tattgttttg
     2641 ccaacagcat ataatcaagg tatgaaagcg acatcaggtg atcaaagtct taaagttgca
     2701 caagtaaatg gtgttatgac cggcattaaa gcacctaaaa atataacaaa gattcaattg
     2761 agctataccc caccatacta ttatttactt ataacaatta ctatatttgg cattatatgt
     2821 agtattattt tcacgagatg ggcaagacaa aaataatgtt gctacgcata tatttaataa
     2881 atctactaag tccttttcaa tggcacttag tagatttttg tttatataag catttttgag
     2941 caaacacgtt atgaataaat aagaaaaaac aattacatta tcaactttac attaactaaa
     3001 cagcaaaaaa agccatctat atcactccat aaaagtgacc tagatggctt tattgtttga
     3061 gtcgaatttg actcatacat agctttgttt aaatgctgat taacgaattt cttggattct
     3121 agcagcttta ccacgtaaac tacgtaagta atataattta gcacgacgta ctttaccacg
     3181 acgtttaact tcgatttttt caatttttgg tgtgtgtaat gggaatgtac gttccacgcc
     3241 aacacctgat gaaattttac gaactgtaaa cgtttcagaa acgccaccgc cacgacgttt
     3301 aattacaatg ccttcgaata cttggatacg ctcacgagta ccctcaatga tacgtacgtg
     3361 tacacgtaaa gtatcaccag gacggaaact tggtaagtct gtacgcaatt gtgatttagt
     3421 tactgcttcg attaatttgt gatttgtcat tataaattct cctcttcaac ctatgttctt
     3481 gcctcgacaa atatatagca gcggatcata gtgatttttg tgtttccaca ctcagaatat
     3541 gttagcacaa tactaacctt ttttcaatcc tattttatat ctttctaata tttgcttatc
     3601 ttcattagtt aatggatatt tttcaattaa gtcaggtctt ttattatatg tgcggatcaa
     3661 cttttgctca tgtctccatg catcaatatt ggcatgattt ccagacaata aaacatctgg
     3721 aactgttaga cccttaaatt cacgcggacg tgtatattgc ggaaactcta ataacccatc
     3781 tgaaaatgaa tcgtcttgat gtgactgttc attacctaaa acacctggaa tcagtctaac
     3841 aatagcatca gtcattgtca ttgccggtaa ctcaccgcca gttaacacat agtcacccat
     3901 tgatatttca tctgtgacaa gatgtgttcg gatacgttca tcgtaacctt cataatgtcc
     3961 gcatatgaaa acgatgtggt cagccttgct taattcaaca gctttctgat gtgaaaatgg
     4021 ctcgccttgt ggacacatta aaataacgcg tgtttgttct gtgacatcta agtcttccat
     4081 cgcattaaaa acaggttcag gctttaacac cataccttgt ccgccaccat acggataatc
     4141 atctacttgg ttgtgcttgt taattgcata atctctaaaa ttaaccgtat tgatttgtaa
     4201 tttattgttt tcttgggcac gtttcataat tgaatgattt aaaacaccat caaacatttc
     4261 aggaaataaa gttaaataat caattttcat taatccaaca atccttccat tggcgtgatg
     4321 ataatttttt tattttcaac atccacttct tttacaacat cagcaatata aggaatcaaa
     4381 tattctttag atcctttaat cacccacaca tcattcgcac ctgtttcaaa tatattaatt
     4441 acacgaccta ttggtgtttc ttgatcatca aaaactgtac atcctataat atctgaataa
     4501 taaaattcat tttcctcaag tacgatatct tcatgatcac gctcttgata aatagaactc
     4561 ccttttaaat gctcaatatc attaatatta ttaatacctt caaatgtaag catatgaagc
     4621 cctttatgca ctctatgtga cttaacagta tattcaaggt cgttattgtt atgcacaact
     4681 gtcagcactt gaccgggttg aaaacgaacg tctgtaaagt ctgaattgga ttttacttta
     4741 atttcacctt taataccatg tgtgttaaca atttgaccaa cttcaactct cattctatta
     4801 cctccaactc tatgctttga acacttctat tttaattgaa aataattcaa agaaaaaggg
     4861 caccattatg gtacccaata cgttctatat tactaacatg tgatattccc aacgtatcat
     4921 tgagtaataa ctgtcagtgt aaacgagtta ttcttattgt tagaacaatt ttacgctaaa
     4981 ttacttagct ttcttttgtt cgtcaaattt tttcataata ccttcttttg ataagatatt
     5041 gtgaactgta tcagttggtt tcgcaccatc atttaaccat tttaaagcta acgcttcgtc
     5101 aactttaatt tctggagcat tagcgctcgt tgggttataa gtaccgattt gttcgatgat
     5161 acgtccgtca cgtggagaac gagcatctgc tactacgata cgatagaatg gatttctttt
     5221 tgaacctaaa cgtgttaaac gaattttaac tgccatttat aaaatctcct ttaagtcatg
     5281 ttttaatttt tattttctac aagaaataat attaacaggt gtatgaatgt ttgtaaagag
     5341 ttttttcttt accaggaaaa ttattgaacc tttttcttac aataataact atgtgattac
     5401 taaattagac tgttaatagg ttataaaaat tcatagtagc cttcaccacc tacgtgttaa
     5461 gttaatttat ctcgtaagga catgaatggt accacttcta acgcacatgt tccaccaata
     5521 taacttatta atattagcaa caataacctc atgttctagc aaatccgcaa tttcagtttt
     5581 taattattat tgtgtatcta aaaatatgca ccccataaag ttatattctt tagtataact
     5641 ttcgggatgc acatctcatt gcaacaaaaa tcagcgtgtt atattaaaca ttaaaacggt
     5701 aaattcatac cttttaacat attttgcatt tgattgcgtt tacctttttt acctttaccg
     5761 ccaccagtga attgtttcat cattttcttc atatcgttaa attgtttcat caaacgattg
     5821 acttcttgta atgaacgacc agacccttta gcaatacgct ttttacgtga tacattcaat
     5881 gtgtctggat tgtttctttc agccggcgtc attgactgga taatcgcttt aatatgatca
     5941 atttgctttt cactcatatt aagcttatct agccctttca ttttattcat acctggaatc
     6001 attttcataa tatcatccag tggtcctaga tttttcacct gatcaagttg ttctaaaaaa
     6061 tcatctaaag taaacgatga ctcacgcatc tttttctcta aatcttttgc tttttcttga
     6121 tccacatctt gttgcgcttt ttcaattaaa cttaacacat cacccatacc taaaatacgt
     6181 gatgccatac gttcaggatg gaatagctct aaaccatcta acttttcact cataccaaca
     6241 aatttaattg gtttttgtgt caccgaacga atagataaag ctgcaccacc acgtgtatca
     6301 ccatctaatt tagttaaggt aacacctgtg acatcaagtt gatcgtcaaa agattctgca
     6361 acattgacag catcttgacc cgtcattgaa tcgacaacta acataatttc gtttggttta
     6421 gcaatttctt ttacttcttt taattcgttc atcaatgctt catcgatgtg taatcgacct
     6481 gctgtatcaa tgattacaaa gtctaaatgt tcttctttag catgttttaa tgcattagtt
     6541 acaatttgtt gtggctttac ttgatctcct tcactgtata caggaatatc aatttgtttc
     6601 cctactgttt gtaattgatt tatcgctgct ggacgataaa tatctgctgc aactaacata
     6661 ggttttttgt tgtatttttt acgcatcaat aatgctaatt tacctgcagt tgttgtttta
     6721 ccagcacctt gtaaaccaac catcataaca acagtaggtg gtttatttga catattaatc
     6781 gatgtatttt ctccacccat caacttcgtt aattcatctt gaactatttt aataacttgt
     6841 tgccctggtg ttaatgattg cattacatcg gaacctaatg cgcgttctga tactgtttta
     6901 ataaattctt ttaccacttt aaagtttacg tcagcctcaa ataacgctaa tcttacttca
     6961 cgcatcatta tctttatatc agcttcagta agtttaccct taccacgcat tttttgcatc
     7021 gtcgcttgca ggcgttctga taagccttca aatgccatat cgatgtcctc cctcaattta
     7081 tatctattct aagtcttcta attgttgaat ataacgttgt atttgttctg gattacttaa
     7141 atgttgtttc atttcatcat atatttctcg gcgttgctca aatttctggt ataattccaa
     7201 tttcttttca taatcttcaa ctaaatcgcc agttcttctt atattatcat aaactgcttg
     7261 tctactcaca ttaaaagtat ctgcgatttc acttaaagaa taatcttcga gataaaataa
     7321 ttccaaataa ttacgttgtt tattcgtcaa taaggattga taaaaatcaa acaaataatt
     7381 cattcgtaac gttttaacta aatcattttg ccccatgatt atcgtccttt tcttctgtta
     7441 caatttgatc attttcaact gttgttattt cgtcattttg ttcaatcata tcagcgaata
     7501 agccgtagac ataactttca gggttaaatg gttgtaagtc atctaattgc tcacctaaac
     7561 ctacatattt aactggaatg tgcaattcat tacgaatggc taatacgata ccacctttgg
     7621 ctgtaccatc taatttcgtt aatacaatac ctgtaacatt tgttacttct ttaaagtttc
     7681 tagcttgtga caacgcattc tgaccagttg tagcatctaa acatagtaat gcttcatgag
     7741 gcgcatctgg cactgctcga ttaattacac gcttaacttt ttctaattct tgcattagat
     7801 ttgttttatt ttgtaaacgt ccagcggtat cacagattaa aatatcaaca cctttgtttt
     7861 tagcggcatt aatcgcatca tacataacag cagctggatc agaaccttca ctttggctaa
     7921 ttacatctac accaacacgt tcgccccaaa ctttcaattg atcaatagca cccgctctaa
     7981 aagtatcgcc cgcagctaac attacttttt taccttccat tttatatcgg taagctaatt
     8041 ttccaattgt tgttgtttta ccaacaccat tcacaccgac cattaaaatg acatttaaac
     8101 gaccatcttc tatgttcata gcttctgaat tatcatcttc ttgatggtaa atctctacga
     8161 ttttttcaac aatgacttca cgcaaatctt cagtatcttg aatattacgt cgttgtgctt
     8221 ccatacgtaa ttcttcagtt aacgtcatca ctgtattaaa accgacgtct gcagtgatta
     8281 acatttcttc taaagcttca aaaaagtctt catctacttt acgatatctc gctatcaaat
     8341 tatttaattg ttcttggaaa ttttgacgag atttttcgag tcctgcttta aatttagcac
     8401 ccattttttg agcttcaatt tcttcaaaat cttcaattga tattaagcca tcgtcatcaa
     8461 aatccgcttc actcaacttg cgtggcttct ttttcacttc agcattttct gctaaatcat
     8521 ttacgtcctg cgttgaacct tcagaatgtg tatcttctaa tttgtcttga ccttgttctt
     8581 ctcttaagga tttaacttct tcattttctt tatttgttgc aaacttatct tttaagcgtt
     8641 taaaaaagct cattattgct cctccttcaa cacatcatct attgtattta aattcacact
     8701 cacaagttta gtaacacctg attcttgcat tgttacaccg tataacctat ctgcaaattc
     8761 cattgttcct ttacggtgtg taataacaat gaattgtgtt tcgtctgata actcatttaa
     8821 atattttgcg tatctaataa catttgcttc atctagtgca gcttcaacct catctaatat
     8881 aacaaaaggt gcagatctta cttttaaaat tgcaaatagt aaagcaatag cagttaatgc
     8941 acgctcacca ccactcagta acgataaatg ttgcaacttt ttacccggtg gttgtaccac
     9001 aatatcaata ccagctgtta aataatcggc ttcagttaat tgcaattctg catcgcctcc
     9061 accaaacaat tgtttgaaca cagctgtaaa atgtccttga atagcatgga aagtttcttt
     9121 aaatctttca gtaacctctt gatccatttc acttataatt tgctctaatg tttctttagc
     9181 tttacgaaga tctgtacgtt gttcacttaa aaatgtataa cgttcattta actcttcaaa
     9241 ttgttcaatt gcatttaagt ttacaggacc tagttcatca atcgacatct tcattaactt
     9301 aactttttta cgtaatgcgt caatcgattc atcactcgta tattcagatt tcgcacgttc
     9361 aacagtcaat tgatattcat catttaaatg atctatcgca tgatgaatta atacatctaa
     9421 ctttgattgt tcagctttaa tatcttggta gtgattttcg atagctaaaa tatcttggtg
     9481 acaaacttgt agtgtagctt cctgcgcatc gatttgttca ttcaactcaa tacgttgttg
     9541 tttcaattta tctaattcat cagataagcg tgttctcgtt tcttgttgac cattaatttg
     9601 atctttaata ttttgaaaag cttgttcgcc catcacttca tccgaattaa agaatgcaat
     9661 tttttcttta acatctttta attgatgttt agtttgttga ctttgattat ttaatcgatc
     9721 tattgtctgt tgttgtgttt taatacgctc tttaaccaca gcaagatcag attgtttctg
     9781 atgtaacgtt tgttgtgttt tagttacgct ttccttacct tctttagaaa gttttgtgta
     9841 gcgttcaatt tcatcttcta gtcgttttaa agatgcttta atactttcta aatgagtttc
     9901 tttttcactc aaagtttgtc gacttttgtc actcgtataa ccatcatttt tttcaaattc
     9961 gaattcttca tgatcatttt ttatttgtgt ttcttgtgta gttaatctat cgagctccat
    10021 ttcaaaatga tgcacttgct ctttaagtgt attatgcttt tgactttttt caaaatacag
    10081 ttcacttaat tgatcacttt ttatcttcaa ctctttaaat tgttgttcaa atgattctgt
    10141 ttgacgcaag taatcttcta attggtgtct cattgttgtc aactcgtctt tttgagacag
    10201 aatacttttg gacttacgag cgccaccacc agtcatagaa ccaccagggt ttacaatatc
    10261 accttccaaa gtaacaatac gagttcgata tttaatcgca cgtgccaatt catttgcatg
    10321 ctttaaatga tcaacgataa tcgtattacc taataaattc ccgataatat tttgatattc
    10381 tggtgctact ttcactgctt ccgaagcaat actaataaat ccgtttgcct ctttagcaat
    10441 agatttaata tcagtcgcta ccactctact ctgtataaca tttaatggta aaaacgtcgc
    10501 acgacctaaa ttacgttctt ttaaaaattg aatagcctgg cgtccatctt tttctgaatc
    10561 tacaatgaca tgttgtaatg aagcacctaa tgctgtttca attgcctgag ttaatttaga
    10621 tggcacatca ataatttccg caactgcacc atgaataccc tttaattctt tatttttagc
    10681 tttcaaaata tgtttgacgc cattgaaaaa ataagtgtat tcttcctctt gcgttgccaa
    10741 actatcaata cgtgttttca ttttttcggt atatcgatat gcttgataca atttctcttc
    10801 gtattcattt tgtgcttttt ttgtatcagt gagatctttt tctatatttt taatttcttt
    10861 atctacagca gaaagttctt tgttggtctg ttgatattct ttttttgtcg tttttatttg
    10921 accctgaata tctttcaatt gctcaaaaac ttcaactaat cgagaatcta gtcttgattt
    10981 tttagcctca ttctcttcta tagtatgctt taaaaaacga atatcattgt taacatctga
    11041 ttgctctgac attaatgtat agtattcgtt tttaatttct tccaattttt catcatgtgc
    11101 ttcgtctgaa acatatagtt gttcttcaag ttcacgaatg acagcattga gttctttttg
    11161 tttacttttc agagacttat aagtatcttg agcttcagaa atctcatttg atatattttc
    11221 taaaagctcc attaaatttt cttgttcttc ttcatatcgt gcatttgttt cagattgatt
    11281 tttcttacgt tcttctaaaa catttaattg tcccgtatat ttttcaaagg cttccgtagc
    11341 ttttactaat tgataattaa gcgattcaac atcgttatca agttgatgtc gtttaccttt
    11401 atattgttga atttgttggc ttaaacgttg cttgtcagct tctttatttg cttgttgacc
    11461 ttgtaaatca tttaaacgtt gatctaattg tctattgtca tttgtatatt gatcaatatc
    11521 gtgcactgta actacaatgt cactatgttt catttgatgt gaaagtgtct tatattcttt
    11581 agctatagct gcctcctctt ttagaggttc tacgcgacct tccaaatcat ataaaatgtc
    11641 ttctactctc gttaaattat cttctgtttg gtcaagttta tttaatgatt cagccttacg
    11701 ttttttatat ttaagtacac cagccgattc ttcaataatt tgacgtctat caattggttt
    11761 agcatttagt atttcatcaa ctctaccttg cgagataatg ctatacgctt cttttcccaa
    11821 tccagaatct aaaaataaat cggcaatatc ttttaatctt gcacggtcat tatttatgta
    11881 gtactcactt tcaccacttc gatacaatct tcttgttaca ataacttcgt tttcatcaac
    11941 actgagcttt ttagaatgat tatctaatct taactgtact tcagcataat tttgagcttt
    12001 gcgatgttct gcacctgaga agataatatc ttccattttt gagccacgta atgatttagc
    12061 cgattgttca cccaacaccc atttaatagc atctgtaata ttacttttac cgcttccatt
    12121 tggaccaaca attgcagtta cacctttatc gaattgaaca ttggtttgat ctgcaaaaga
    12181 cttaaatcca atggcatcta ttgattttaa ataaaccatc ctaaactcct tatcacaatt
    12241 ttaaagaaca catgtatacg atattttaat atttgtatat taaatttcga agttgttcca
    12301 taaaatatta aaaattcttc cttaaatcct aaatcgaaca cctatatgtc gtatcatata
    12361 aatttctatt taatttgttt taattgctta taggcacttt cagcagcacg ttgttctgat
    12421 tcttttttcg ttttcccttt accttcagct attgcttccc cttgcagaat aacttctgaa
    12481 gtgaatagac gatgatgtgc cggtccctct tcttttatta aattataggt tacatcacct
    12541 ttattttgct ggtgcacata ttcttggaat tgtgttttaa aatctaccac gcctaataac
    12601 tcattttgtt ctacatgtgg gaaaataact ttctcagcaa atttccaaac tatatctagt
    12661 ccttgatcca aatacaatgc cccaataaat gcttcgaatg catctgatat taatgatggt
    12721 cttgtacgtc cccctgtttt ctcttcacct ttaccaagta aaatcatttc gttcaatcca
    12781 attttattcg caaatattac aagtgagggc tcacatacaa tagtggcacg catttttgtt
    12841 aaattccctt ctggcaagtt gggatgttta tcaaataaat atcgtgaaac cgtcaattct
    12901 aataccgcat cacccaaaaa ctctaaacgc tcattatggt ctaaacgatt catattaaaa
    12961 tcattaataa aactcgaatg cgaaaatgct tgttggtata aatcaatatt ttgataagta
    13021 aagcctaact ctgtcatttt agtatcaaag cgctttctaa aacgattaac tatctcactt
    13081 ttcttttgtt tagacattat tttatctcct ttatgtgttg ctcttgaatc atctttaatt
    13141 ttacgttaaa actattgata attaaagaaa aaaactgagt cgacaatact gacgacccag
    13201 atgtaagatt tattattttt caagactgtt aataaattta acagcatcac caacagtgtt
    13261 gattttttca gcttcttcat caggaatttc agtaccaaac tcgtcttcta attccattac
    13321 taattcagcg atatcaagtg agtcagcgcc taaatcatct ttgaaagatg catcttcagt
    13381 tactttatca gcgtctacac ctaaacggtc aacgatgata tcttttactt tatcgaaatt
    13441 ttccacgtcg attcacctcc tttaaaaatg cctatacata gactattata ttttcccatt
    13501 ttaaattcta aaaaacaagt agaaatttta gaaacactta tcaatttatt gattagtgtt
    13561 tcttatgcat gagctttagc tcaggtgtac ctctatccca gttatcaatt tattgattac
    13621 tgcgtcttta tcttgagctt tagctcaggc gtacctctat cccagttatc aatttattga
    13681 tttctgcgtc tttatcttga gctttagctc aagcgttcct ctaatcttct tatcaattta
    13741 ttgattagtg tttcttaggg agaaattctg gaaccttatc taggttgctt agaatttccc
    13801 ccgcaaagtt gactaggtca gacaattctc cattggatga ccagtcaacc actgcgtcaa
    13861 tgagctttag ctcaaatata ttacatgtac attccaccat ttacatggat tgtttgacct
    13921 gtaatatatt ttgctttgtc tgatgctaag aacgctactg tattagcaat atctgtgtct
    13981 tgaccaaaac gtgctaacgg aatttgagtc aacatttgtt ctttaagctc atcacttaaa
    14041 gcatctgtca tatcagaaac aataaaacca ggtgcaactg catttacagt gataccacga
    14101 gatgctaatt cacgcgccgc agatttagtt aaaccaataa cacctgcttt tgttgcaaca
    14161 tagtttgctt gtcccggatt acctactgct ccaacaacac ttgataaatt gatgatagca
    14221 ccactacgtt gtcttaacat ttgtggtgtt gctttttgga tacagttaaa tacacctttt
    14281 aagtttgtgt caataacatc atcccactct tgttctttca tacgcattaa taaattatcg
    14341 cgagtaatac ctgcattatt tactaaaaca tctaaagaac caaattggct aactacttct
    14401 ttaatcattg ctttaacttc atcagcatcg gcaacatttg cttgaatcgc aaaactgtca
    14461 acacctttag ctttgatttc ttcgactact gcttcagctt tctctttgct gcctgcatag
    14521 tttactgcta cattatatcc ttcttctgct aattgtaacg caatactacg tccaattcct
    14581 cttgatgcac ctgttactaa agcactctta gtcattttca ttccatcctt tcacatcttc
    14641 taaagtttga attgatgtta acttaacatc tctatttatt tttttaatta agccagataa
    14701 aacttttcca ggaccaattt caataaaatg atcaacacct tggtctatta gccattctgt
    14761 tgagttaatg aattgtactg gtgaatataa ttgcttgacc atattagatt taattacttc
    14821 tttgtcagtt tcaccttgcg catttacatt ttgaactaca ggaaacttag catcacgcca
    14881 ttcaaattga ttaatgtaac ttgaaaaatc ttcttcaatc actttcatta gcgatgaatg
    14941 gaatggtcca gatactgcta aaggcatgac acgttttgca cctaatgatt tacctttttc
    15001 tactagctca tcaattaaag ctttgtgacc tgaaacaaca atttgacctg ggcaattaat
    15061 gtttgctggt tcaattattt tgtcatcaga tgataatgac ttacaaattt catcgacttt
    15121 atcaaaatct aatcccaata ctgcagccat gcttcctaca ccagtaggaa atgcttgcgc
    15181 cattaattga ccacgttttc taacaatttt aactgcatct tcaaatgata atacgtcagc
    15241 tgcaactaaa cttgaatatt cacctaaact atgccccata gtaaaatcag gattcaaatt
    15301 ttttagcgct gctaataatg ccgaactatg cgtcaataaa gctggttgcg tgttttcagt
    15361 ttcacccaat ttaccttctt catcagtaaa cattgtctct aaaatatcaa agtctaacgt
    15421 ctttgctgct gaagttaaaa tttcagttgc ttgatcattg ttattaaaca aatcttgtgc
    15481 cataccaact ttttgggcac cttgtcccgg aaaaataatt gctgttttac tcatttgatt
    15541 cacctacagt ctctttcatt gtttgtacaa tattttgttc tcctgcgatt ttcgcttgtc
    15601 taattgcaga ataaaaagct ttagcatttg aactaccgtg tgctttaact acagtacctt
    15661 ccaatcctaa taatactgaa ccaccgtatt ctgagtaatc catcttttta gcgaattctg
    15721 ctaaatcttt cttcaatatt gcacctgcta atttattttt agtactactc ataatcgtat
    15781 cttttaacat tttaccgatt gattttgcag taccttctaa atttttaagg accatgttcc
    15841 cagtatagcc atcggtaact acaacatctg tatcgccatc cattaatgtc ttcgcttcaa
    15901 tattcccaac aaaattcaat gaatgatctt gatttaataa ctcaaatgat tttttcgtta
    15961 aactattacc tttagctggc tcggtaccta tatttaataa tgagattttc ggattatcaa
    16021 tacctctaat tttttgagca taaatatccc ctagttgcgc atactgtaat aagtgttcag
    16081 gtttagcatc agcatttgca ccaacgtcta aaaagacaaa accttttcca tcaatcgttg
    16141 gcaatgttac tactaaagcc ggtctagcta cacctttaat acgtccaaca atgaataaac
    16201 caactgacat taaagcacca gtattacctg ctgacacaca tccatctgct tcaccagatt
    16261 tcacagcttc agccattttt accattgagc tatctttttt acgtttaatc gctctaacag
    16321 gctcatcttc catttcaatc ttttcagaac aatgtctaaa ttcgattcgt tcatggttca
    16381 gattatactt tttttcgtca ccgaaaagca taatttctag atttttaaag tcttcaacag
    16441 ccttttgtac ggcttctaat acgatatcag gcgcattgtc gccacccatc atatcaattg
    16501 ctaatttaac cataatttta tcctcgctta tcataaaaca ttttaaaatt tcctttgaaa
    16561 actaatgtat gtttaacata tgactttact tcgacgtaat aatgttttgc agtttgattt
    16621 acaactcgtg cttctgctct taccgtatca tttaatttta ctttttcaat aaattgaatg
    16681 ctactctcat gagttaaaac tgttggttgc ttaattagcg caacacataa cgaatttgcc
    16741 tgagcaaaca gcacatgacc acgcgcaatt ccagttttat gaaaaacaga atccgatgta
    16801 atatctaaaa ttgattgcgc tttaacattt ggattgactt gaatcaaatc accaataaat
    16861 tcttgttctt caatagaact tatttggtca taattctttt cagcaactaa tttaatacgc
    16921 ttccttaatt ctggtatgtt taaataagtg cgatctaaac gaattgtttg tatactcact
    16981 tgaaataagt cgcttagttc atggtctgtg atgaagggat tgctatcaat ttgttgtctg
    17041 attgcttctc tacgtttatc tttctttagt ttcaacgtct cacccctcat tttttagtac
    17101 ctagtcttaa acattcctca catatcataa cactttctat ttaaccgact caacaaataa
    17161 ctgtattatt tagctattat ttagtcggaa atttataaat tgttaaaata ccactttaca
    17221 tttaaatagt tcaagtgtca tatcactcaa ttaacgaagt gttgcactca aattgacaaa
    17281 tcagcatggt aattagtcaa aactgcgatg taataaattt tcttcaacaa aatgacgtaa
    17341 atgttgatac gtattttcaa agaatacgcc agattgaata agttcagctg cttcatcacg
    17401 agcaacttct aacatacgat aatcttcaac taaattggca actaagaaat ctggcaaccc
    17461 actttgttta acaccaaaga aatctccagg accacgcatt tctaagtctc gttcactcaa
    17521 ttcaaatcca tccgttgttt gtgtcataat tgtcattctt tcaattcctg tttctgtttt
    17581 aggggatgca attaaaacac agtaactttg ctggtcactt ctacctacac gaccgcgtaa
    17641 ctgatgtaaa gttgataatc caaagcgatc cgcatcataa atcatcataa aagttgcatt
    17701 cggtacatta acacctactt caacaacagt agtagaaact aaaacatcta tctcatgatt
    17761 actaaacttt tgcatgacct catctttttc atcggcagat aacttaccat gcaataaccc
    17821 tacacgggaa acaccataat actgttgtaa agactcgtac aatgcgacaa cattttgaac
    17881 atcttcgaga tgctcagaac tttctattag cgggcaaatg acatatgctt gacgaccttt
    17941 tttcaactct gaggtcattt gcatcaaaac tttatcgtat tgctcatgct ttgcccaagt
    18001 agtaatgata ggtttacgac cttttggtaa ttgtttaatt gaagacacat ccatctcacc
    18061 aaaaactgat attgctagtg ttcttggtat cggcgttgct gtcataaata acacattcgt
    18121 cattgcacct ttttctctta aaagctggcg ttgattcaca ccaaatcgat gttgttcatc
    18181 tgtaattact aaaccaacat tatggaaaat cacatcatct tgaatcaaag catgggttcc
    18241 aattaaacaa tcaatcgtac cattttcaag ttgttctaaa agtatctttc gtttcttacc
    18301 ttttactgac ccagttaaca atgcaacgtt catagaatct ccaaataaag ccattaaact
    18361 ttcagcatgt tgctctgcta aaatttcagt tggtaccatc aatgctgatt gataaccagc
    18421 cgtttttaac gcatacatac aaattgcagc aactactgtt tttcctgaac ctacatcacc
    18481 ttgaagtaat cgatgcatac gtattggtgc ttttaaatct ctaaaaattt cattaacact
    18541 ggatttctgt gcttcagtta gttcaaaagg taaacgatca ataaatgatt taacttggtc
    18601 taagtcataa tcaatttcaa ttgcttcgtc agatgacttt tctaatctat ttagccattg
    18661 catacgtaat tcgaataaaa acagttcagt aaatgcatag gttctacgag cacgtaataa
    18721 atcctcttta cttttaggat gatgtaatgt gttcaaagta aagtccaagg tctctaattt
    18781 atatttttct cttagttcat cagttaacca ttcatgaatt gtcacatcat ttaacgcttg
    18841 tctaatttgg tctcgtattt gcttttgttt aataccttcc ttaatacgat agactggttc
    18901 taattgaaca tctgcgtttt cttgagtttg tgtcccttgt gaattaaaga aaaccctatt
    18961 accagtaatt tcctgtttaa ctctattcca cttaccttta acagttatag tttgatttaa
    19021 ttcgattttc ttttttaaat acggttgatt gaaaaaaata catttgacag caatattatt
    19081 taccattaaa tgaacggtta attttgattt atttcttcca aaaaatgcaa ctactggagc
    19141 tgtatatact tgtccttcta tcgtaacgtt agattgatct tctgcttgat tcaaatcaat
    19201 cactgtatta tcttcatatc tagttggcaa ataaagaaca agatcttcca ctgtatgaat
    19261 atttagttgt tgcaatactt ctattttctt aggacctata ccttttaatt gtaaaagaga
    19321 atatggactt tctattaagt ttactttagc caaaataaat cacctatgtt ttatttgatt
    19381 ttacgtatca tttagtatgt tatttatttt atcatgacta agttgaacat gacattgtta
    19441 gcaactcgag aatgatactt ttaacccgca ataacactaa aatagcaaaa taaaaaacta
    19501 ccaactgatg atttatcatt ggtagttttt tattttaaat ttttattcta ctgaaaagaa
    19561 atattgataa attggttgtc caccttcatg aacttccact tctacatctg gatattgctc
    19621 ttcgatccag tttatcatgt tatctgtaac tgcttgctct gcatcttgac caataatcac
    19681 agtcaatatt tcactatctt ctgctaacat ctcatttaac aactcagtaa ccgttgttaa
    19741 ttgatcactt tggctgctta caatcttatc ttcaatcaag cccataaacg cgtctttttt
    19801 aatctcaacg ccatcaattt tcgtatcacg aacagcgtac gttaatgaac cagatttaac
    19861 gttatttact gaatcagcca tttgcgcttt attttcttca agtgttgcgt ccacatcata
    19921 ttggaatagt gcgcttatac cttgaggaat agatttcgtt ggaataacaa ctgcttctgc
    19981 atcaacaata ctcgctgctt gttcacttgc cattaaaata tttttattat tcggtaaaat
    20041 aattgcacgt ttacattttg attgttcaat gactttaacg atatcttctg tagaaggatt
    20101 catcgtttgt ccaccactaa tgatatgtgt ggcacccatt gatttgaata tctctgaaat
    20161 accttcaccc atagaaatag taataattgc tgtttcaacc gtttccattt tcggtttagc
    20221 tgtgtgctgt tcttttcgaa tcacttcacg gtgctgttct ctcatatttt caaccttaag
    20281 tttaattaat tcaccatatt gttgaccata attaaacact ttacctgggt attcggtatg
    20341 cacgtgaact ttcacaattt cttcatcatt aatgactaat aaagaatcac caaattgact
    20401 catatcttgc ctgaattctt gttcatcaaa ggctttttta ttctttccaa aacgaaccat
    20461 catttcagta caatagccat aaataatatc ttcagtatta attacaccat ggaaatcatg
    20521 ttcatcatgt acaaattcat ctttatctat ctttgcaact ttggcttcaa ctttttcacc
    20581 tttaagcgct tttaagaatc cttcgtaaac gcataacaaa cctttaccgc cactatcaac
    20641 aacaccaact tctttaagta cagctaataa gtttggtgtg ttttcaagtg attcattggc
    20701 ttttacaata atgtactcca ttaattctat acaatcttca gtattatttg ctttttctat
    20761 tgcagcttgc gcagcatctt ttgcaactgt aagtattgta ccttcaactg gtttcataac
    20821 agctttatat gccgtttcaa caccagcttg aaaactttca gctaacaatt ttgaattaat
    20881 ttcagattca ctttcaatat ttttacaaaa tcctctgaat aattgtgaca agatgacacc
    20941 agagttacct cttgcaccca ttagtaaacc tttcgagaat gttttaccta attcgccgat
    21001 atttttcgac aaattattct ctacttcttc gcgacctgaa gtcatagtaa gattcatatt
    21061 tgttcctgta tcaccatctg gcactggata cacattcaaa gaatctacca aatctgcatt
    21121 gttagataaa ttttgtgccc cttgtataat catatcggca aataatttac cattaatttt
    21181 gctaatcatt tcaagttgtc ctcctaagct ttcttgcctg tattattcac acgtacacct
    21241 tgtacatata tattgattga atttactgat acattaagtg atttttccaa agtatatttc
    21301 actgttgatt gtacattatt ggcaacttca gatattttca caccataact tacaataatg
    21361 tacatatcta tatccactac gccattattt tcagttactt tgatgccttt agcataattt
    21421 tcatgtccta gtatttccgc aataccatct ctaacttgtt gtctagatgc catacctaca
    21481 ataccataac attcaacggc ctttccacct acaaccgaag caatcacttc gtttgaaata
    21541 tcaattttac cgtaatcatt tgaaatctct aatgtcatat ctttgtccct cctaaatttc
    21601 aaagtttatt attcgttttc cctaatcact tttaaaattt taccacttag tgtagtcata
    21661 aacgctttaa gtttcattga ttatttttat aaaatatcaa tggtacaact tatcagaatg
    21721 acttacgctt aaatcaccta ttatattttg agtaaactga atttacactc atgacgatta
    21781 tctataggcg ttttctttta atagatgtag tatcattaaa taaaaatttt aaatgtttca
    21841 ttcacttatt tctctaaaat atcatactac ataacttcac agtctacaat tatatcacaa
    21901 aactcagtat ttttgacttt tatgtgaaaa ttattcattg tttttcaaat gaaactatgc
    21961 tatct
//
LOCUS       DAHHSC010000021        21814 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.021, whole genome shotgun sequence.
ACCESSION   DAHHSC010000021 DAHHSC010000000
VERSION     DAHHSC010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 21814)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21814)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21814
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.021"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            complement(107..1336)
                     /locus_tag="NBI57_000651"
     CDS             complement(107..1336)
                     /locus_tag="NBI57_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HCD1821618.1"
                     /translation="MGRNLKLKKESDFEFTKNHKRLLLGSVFLMATSAIGPAFLTQTA
                     VFTSQFFASFAFAILLSIIIDIGAQINIWRILVVTGLRGQEISNKVVPGLGTVISILI
                     AFGGLAFNIGNIAGAGLGLNAIFGLDVKWGAAITAIFAILIFVSKSGQKIMDVVSMIL
                     GIVMILVVAYVMFVSNPPYGDAFVHTFAPEHPMKLVLPIITLVGGTVGGYITFAGAHR
                     ILDSGIKGKQYLPFVNQSAIAGILTTGIMRTLLFLAVLGVVVTGVTLSSENPPASVFE
                     HAIGPIGKNIFGIVLFAAAMSSVIGSAYTSATFLKTLHKSLNERSNLIVIVFIVISTM
                     IFLFIGKPISLLIIAGAINGWILPITLGAILIASKKKSIVGDYKHPNWMFIFGIVAVL
                     VTILTGIFSFKEVLQLF"
     gene            complement(1349..2101)
                     /gene="pxpA"
                     /locus_tag="NBI57_000652"
     CDS             complement(1349..2101)
                     /gene="pxpA"
                     /locus_tag="NBI57_000652"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HCD1821619.1"
                     /translation="MRVDLNCDLGEAFGNYSFGGDHQIIPLITSANVACGFHAGDENV
                     MNETVKLAKAHNVAVGAHPGLPDLKGFGRRNIDISNDEIYNLMIYQLGALQGFCRIHQ
                     LKINHVKPHGALYQMGAKDREIANVIAQAVYDFDPSLVLVGLANSYLISEAKNVGLIT
                     ASEVFADRRYEDDGQLVSRKESDAVITDTDEALKQVLKMVKENKVISKNNKEVTLQAD
                     TICVHGDGEHALLFVSKIREILMKEGIDIQSL"
     gene            complement(2101..3462)
                     /locus_tag="NBI57_000653"
     CDS             complement(2101..3462)
                     /locus_tag="NBI57_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HCD1821620.1"
                     /translation="MLRCLIANRGEIAVRIIRACREYGIETVAVYAKGDEQSLHVHLA
                     DQAICIGEANALDSYLNIDRIISAAQITGANAIHPGYGFLSESTKFAQTVEEQGIAFI
                     GPTKKTMEMMGDKITARQTVHHAGVPVIPGSNGAVNHVSEIENLAKDIGYPVVIKAAS
                     GGGGKGIRIVKKAEDLEKAFKEAKSEGKKYFDDDRVYVEAFIPVAKHVEVQVMGDGQD
                     NYVHLGERDCSVQRKNQKLIEESPCAALTEERRQQICNDAVKVARAANYRSAGTIEFL
                     VTDTAHYFIEMNARIQVEHTVTEMRAERDLVAAQLYLLEHNHLPFSQSDIQFNGHVIE
                     ARINAENPEKKFQPTPGKVTALHLPQGFNVRVDSLLYHGYQVSPYYDSLVAKVIVKSH
                     DRASAIDKLKVTLDEMVIDGFSTTADFLYAVLNYPLYRDGDAKDVDIKFLEKHQIVKG
                     VEL"
     gene            complement(3476..3925)
                     /locus_tag="NBI57_000654"
     CDS             complement(3476..3925)
                     /locus_tag="NBI57_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein subunit"
                     /protein_id="HCD1821621.1"
                     /translation="MNIEKIEQIIKLVKENDVKKFKYKNFEDEIEIDFTDSNHLAAHS
                     NQSNQSMNNNDLTASKANDNSDVSTNDYHDIKSPMVGTFFLQDSKELTEPIVNVGDKV
                     NKGDIIGYVEAMKVLNEVTTDVAGEITEIVADHGTNVEYDQVLVRIK"
     gene            complement(3927..4937)
                     /locus_tag="NBI57_000655"
     CDS             complement(3927..4937)
                     /locus_tag="NBI57_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000023326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-dependent carboxyltransferase family
                     protein"
                     /protein_id="HCD1821622.1"
                     /translation="MSIKILQPGLFSTVQDLGRIGYEHIGFSGAGAMDQFSFKVAQSL
                     INNDGPAIEYTLIGPTIQFNTQNTFVITGGSINASLNNKTISMNSVILAEKGDILKIG
                     AITKGVRGYLTFGHSINVPSIAESYATHTRSSIGGFKGRKLLANDVITVKINNDFKEN
                     IGKTIHLQDDLLPENNIIHILQGPQFEAFSEEARAKIVNHPYLITEQSDRMGYRLEGD
                     SVAPINQADIISEPVALGSVQVPNDGQPIILLNDKQTIGGYTKIATVCKFDLPKLAQM
                     KPKDTIQFKWISFQEAVDKNREQMSLFNEILKSHQKTPIFDTSSLRHTSKKLSTILKG
                     DL"
     gene            complement(4927..5661)
                     /gene="pxpB"
                     /locus_tag="NBI57_000656"
     CDS             complement(4927..5661)
                     /gene="pxpB"
                     /locus_tag="NBI57_000656"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001617363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HCD1821623.1"
                     /translation="MDVRFINEQTIMIYFENKISEETYRNVTAMVRWIREKEILEIQD
                     IVPSYRAVLIYFDEQAITSSKLIENLELNKFNEKNVHAVNQTNRIIKIPVQYGGTYGP
                     DIEEVAKHNRITVEQVIEKHTSKPYLIYMLGFMPGFPYLGGLDEQLHTPRRNQPRLKI
                     HAGSVGIANNQTGLYPSDSPGGWQIIGRTPLKVFSSEREPMSMYEAGEWIQFYAIDEQ
                     KFIQIERDISDGNFNVDDWVVIENVN"
     gene            complement(5987..6463)
                     /gene="greA"
                     /locus_tag="NBI57_000657"
     CDS             complement(5987..6463)
                     /gene="greA"
                     /locus_tag="NBI57_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HCD1821624.1"
                     /translation="MENQKQYPMTQEGFEKLERELEELKTVKRPEVVEKIKVARSFGD
                     LSENSEYDAAKDEQGFIEQDIQRIEHMLRNALIIEDTGDNNVVKIGKTVTFVELPGDE
                     EESYQIVGSAESDAFNGKISNESPMAKALIGKGLDDEVRVPLPNGGEMNVKIVNIQ"
     gene            complement(6491..7114)
                     /gene="udk"
                     /locus_tag="NBI57_000658"
     CDS             complement(6491..7114)
                     /gene="udk"
                     /locus_tag="NBI57_000658"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HCD1821625.1"
                     /translation="MKATTIIGIAGGSGSGKTTVTNEIMKNLEGHSVALLAQDYYYKD
                     QKHLTFDERLETNYDHPFAFDNDLLIENLKDLKNGKAVEVPTYDYASHTRSDITIDFK
                     PKDVIIVEGIFALENKVLRDMMDVKIYVDTDADLRILRRLTRDTKERGRSMDSVINQY
                     LSVVRPMHDQFIEPTKKYADIIIPEGGSNKVAIDIMTTKIQSLVSKQ"
     gene            complement(7114..8382)
                     /locus_tag="NBI57_000659"
     CDS             complement(7114..8382)
                     /locus_tag="NBI57_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006190454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HCD1821626.1"
                     /translation="MKTIEEIKSTPKTVMKKPELLAPAGNLEKLKIAVHYGADAVFLG
                     GQEYGLRSNADNFTMEEIAEGVEFANRYGAKIYVTTNIIAHDENIEGLESYLRNLEKT
                     GATGIIVADPLIIETCKEVAPKLEIHLSTQQSLSNYKAVEYWKEEGLDRVVLARETGA
                     MEMREMKEKVDIEIEAFIHGAMCIAYSGRCTLSNHMTARDSNRGGCCQSCRWDYELLE
                     VDDNGELDVFYNQGEVTPFAMSPKDLKLIESIPQMMDIGVDSLKIEGRMKSIHYIATV
                     VSVYRKVIDAYAADPDNFKINPEWLIELDKCANRDTAPAFFEGTPGYEEQMFGQQQSK
                     KSPFDFCGLVLDYNEDTKIATIQQRNNFKPGQEIEFFGPEIETFTQVVEAIYDEEGNS
                     LDAARHPLQIVQIKVDRPIYPNNMMRKEIG"
     gene            complement(8394..9317)
                     /locus_tag="NBI57_000660"
     CDS             complement(8394..9317)
                     /locus_tag="NBI57_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HCD1821627.1"
                     /translation="MTELLVTPKSLSHMETLIDLGADAFVIGEQKFGLRLPGEFNRQQ
                     MTEAVALAHKNDKKVYAAVNGLFHNYHLDAVEDYINFLHEIRVDRIIFGDPAVVMYVK
                     AQENPIPLHWNAETLVTNHFQCNYWGKRGASRAVLARELNLDEIINIKENSNVEIEVQ
                     VQGMTCMFQSKRMLLGNYYTFQDRQMKIERRNDEQSLLLYDEERQNNYPVYEDYNGTH
                     IMSPNDICLIEELAPFFEAGIDSFKIDGILQTEEYINVVTEQYRQAIDLYNEDPEIYE
                     DEKFMLIDPIEEIQPDHRPFDEGFLYKQTVY"
     gene            complement(9320..9958)
                     /locus_tag="NBI57_000661"
     CDS             complement(9320..9958)
                     /locus_tag="NBI57_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HCD1821628.1"
                     /translation="MDDLNKKYLIDLHQHQNSSIEVLREFAEGNEVPIVDRLTLDLIK
                     QLIRMNNVKNILEIGTAIGYSSMQFASISDDIHVTTIERNETMIQYAKQNLATYHFEN
                     QVRIIEGNALEQFENVNDKVYDMIFIDAAKAQSKKFFEIYTPLLKHQGLVITDNVLYH
                     GFVSDIGIVRSRNVRQMVKKVQDYNEWLIKQPGYTTNFLNIDDGLAISIKGE"
     gene            complement(10243..10551)
                     /locus_tag="NBI57_000662"
     CDS             complement(10243..10551)
                     /locus_tag="NBI57_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HCD1821629.1"
                     /translation="MTEHNHDSQLEINNEEELLTLFDEEGNEVLYRKVLEFYHPEFKK
                     EYVILAEEGAQSDEDDMIELVPMINEPDESGDGGKLVPIETDEEWDMIEEVVNTEMEE
                     "
     gene            complement(10566..10994)
                     /gene="ruvX"
                     /locus_tag="NBI57_000663"
     CDS             complement(10566..10994)
                     /gene="ruvX"
                     /locus_tag="NBI57_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HCD1821630.1"
                     /translation="MLQHKILGLDVGSRTVGIAISDIMGWTAQGLDTLRINEENNELG
                     IDQLVDIIKKHNVGTVVIGLPKNMNNSIGFRGEASLTYKEKLLEAYPSIEIVMWDERL
                     STMAAERSLLEADVSRQKRKQVIDKMAAVFILQGYLDSLH"
     gene            complement(10998..11258)
                     /locus_tag="NBI57_000664"
     CDS             complement(10998..11258)
                     /locus_tag="NBI57_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HCD1821631.1"
                     /translation="MENFDKTMKFDYEELPTQDVRDVLNNVYRTLDERGYNAVNQIVG
                     YLLSGDPAYIPRQNEARNQIRHIDRDVIMEELVSYYLKEQNK"
     gene            complement(11321..13951)
                     /gene="alaS"
                     /locus_tag="NBI57_000665"
     CDS             complement(11321..13951)
                     /gene="alaS"
                     /locus_tag="NBI57_000665"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HCD1821632.1"
                     /translation="MKKLKASEIRQKYLDFFVEKGHMVEPSAPLVPIDDDTLLWINSG
                     VATLKKYFDGRETPKKPRIVNSQKAIRTNDIENVGFTARHHTFFEMLGNFSIGDYFKQ
                     EAIEFAWEFLTSDKWMGMEPDKLYVTIHPEDMEAYNIWHKDIGLEESRIIRIEGNFWD
                     IGEGPSGPNTEIFYDRGEAYGQDDPAEEMYPGGENERYLEVWNLVFSEFNHNKDHSYT
                     PLPNKNIDTGMGLERMASVSQNVRTNYETDLFMPIMNEIEKVSGKQYLVNNEQDVAFK
                     VIADHIRTIAFAISDGALPANEGRGYVLRRLLRRAVRFSQTLGINEPFMYKLVDIVAD
                     IMEPYYPNVKEKADFIKRVIKSEEERFHETLEDGLAILNELIKKAKATTNEINGKDAF
                     KLYDTYGFPIELTEEIAVQAGLKVDMTTFESEMQQQRDRARQARQNSQSMQVQSEVLK
                     NITSASTFVGYDTATAQTTLTHLIYNGEEVSQVEAGETVYFMLTETPFYAVSGGQVAD
                     TGIVYNDNFEIAVSEVTKAPNGQNLHKGVVQFGQVNVGATVSAEVNQNDRRDIQKNHS
                     ATHLLHAALKSVLGDHVNQAGSLVEADRLRFDFSHFGPMTNDEIDQVERLVNEEIWKG
                     IDVNIQEMDIASAKEMGAMALFGEKYGDVVRVVNMAPFSIELCGGIHVRNTSEIGLFK
                     IVSESGTGAGVRRIEALTGKAAFLYLEDIQEKFNTMKSQMKVKSDDQVVEKLTQLQDE
                     EKALLKQLEQRDKEITSLKMDNIEDQVEEINGYKVLVTEVDVPNAKAIRSTMDDFKSK
                     LQDTIIILASNVDDKVSMVATVPKSLTNNVKAGDLIKQMAPIVGGKGGGRPDMAQGGG
                     TQPENISKSLSFIKDYIKNL"
     gene            complement(14294..16771)
                     /locus_tag="NBI57_000666"
     CDS             complement(14294..16771)
                     /locus_tag="NBI57_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HCD1821633.1"
                     /translation="MSDPTLFDYSMIKGTVEAILFQNSDNFYTVLKVDTIETNEDFDT
                     MPTVVGFLPNIVEGDVYTFKGQVVDHPRYGKQLKAETFEKEMPQTKEAIISYLSSDLF
                     KGVGKKTAQNIVNTLGDNAINDILDDHSVLEKVSGLSKKKQKQIAEQISANQESEKIM
                     IRLHDLGFGPKLSMAIYQFYLGDTLTILDRNPYQLIYDIKGIGFNKADQLARNIGIAY
                     NDNERLKAALLYTLEEECIKQGHTYLPINVVIDLTVDVLNYQDEEVIEPEKLDEMLQY
                     LNEEKRLIIDNEQVAIPSLYYSEIKSVQNLFRIKTHTNKLTEIEQSDLQMHIGEIEDA
                     NQVNYAASQKEALQTAINSKVMLLTGGPGTGKTTVIKGIVELYAEIHGLSLDYDDYVN
                     DDYPVVLAAPTGRASKRLQESTGLEAMTIHRLIGWNQDTKPEDILENEINARLIIIDE
                     MSMVDTWLFHQFLSAVPLDAQLIFVGDEDQLPSVGPGQVFKDLIESKAIPRVNLTEVY
                     RQQDGSSIIELAHRMKLGQKIDITQRFHDRSFINCQANQIPTVVEKVVTSAVNKGYTM
                     ADIQVLAPMYKGNAGIKRLNQVLQDILNPKKKDTREIEFGDVVFRKGDKVLQLVNRPN
                     DNIFNGDIGVIVGIFWAKENALNKDVLVVDFEGNEITFTKQDMMELTHAYCTSIHKSQ
                     GSEFPIVIMPIVKQYFRMLQRPILYTGLTRAKTSLVLLGDPEAFDIGLKTNGQARLTQ
                     LCTLLKNYFNSEDEEMLENTATNDTGASQTTIDDQVEAPMSSNDSGEVTSDSTKTDIN
                     VLTEATMFKIDPMINMGEITPYDFIER"
     gene            complement(16773..17441)
                     /locus_tag="NBI57_000667"
     CDS             complement(16773..17441)
                     /locus_tag="NBI57_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HCD1821634.1"
                     /translation="MIDQQTIYQYIQNGKIEEALQALFGNIEEDPTIIENYINAGIVL
                     ADANEIEKAERFFQKALTIDPKNGVVFYNLANVYYNQQRYQEAIKLYQQALQTEIEQV
                     DCNYMIGMAFNQLESFKLALPYLMTAAELDKDKDAEVQFQYGLVLCQLEMFNEAITQL
                     KHVLTIDKNHVDARYNLGLALFMKNEDIDEAITHFKEAVTIDPKHLLSQHALKTFTKM
                     KEEE"
     gene            complement(18017..19135)
                     /gene="mnmA"
                     /locus_tag="NBI57_000668"
     CDS             complement(18017..19135)
                     /gene="mnmA"
                     /locus_tag="NBI57_000668"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001830892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HCD1821635.1"
                     /translation="MSNKDIRVVVGMSGGVDSSVTAHVLKEQGYDVIGIFMKNWDDTD
                     ENGVCTATEDYNDVIEVCNQIGIPYYAVNFEKEYWDKVFTYFLDEYKKGRTPNPDVMC
                     NKEIKFKAFLDHAMNLGADYVATGHYARIHRHEDGHVEMLRGVDNNKDQTYFLNQLSQ
                     QQLSKVMFPIGDIEKSEVRRIAEEQGLVTAKKKDSTGICFIGEKNFKTFLSQYLPAQP
                     GDMITLDGKKMGKHSGLMYYTIGQRHGLGIGGDGDPWFVVGKNLKDNVLYVEQGFHHD
                     ALYSDYLIASDYSFVNPEDNDLDQGFECTAKFRYRQKDTKVFVKRENDHALRVTFAEP
                     VRAITPGQAVVFYQGDVCLGGATIDDVFKNEGQLNYVV"
     gene            complement(19136..20278)
                     /locus_tag="NBI57_000669"
     CDS             complement(19136..20278)
                     /locus_tag="NBI57_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HCD1821636.1"
                     /translation="MEIYADYAATTPVKPEVVDAMMMIYNSHYGNPSSIHAKGRDARK
                     YLDESRRQIAQLLGADTHEIIFTSGATESNNTAIKGIVKANEQLGNHIITSKIEHHSV
                     LHVFEQLEREGFDVTYLDVDDTGAIDLDQLEETITDKTILVSIMFVNNEVGTVQQIYD
                     IQDIIAETNAYFHVDAVQAIGHLDVKFDEFEIDAMSITAHKFGGPKGVGALLVKDHVT
                     LDYPQLGGEQELKRRAGTENLAQIVGMAKALQLAEKNRDDNNIHLMNLKEQFLVKLQE
                     RAIPFELNGSMTDATGHIVNLYFPFVEVEMMLTLLDMAQIYVSSGSACTAGSTQPSHV
                     LDAMFEDEERSNHSIRFSFNELTTENEINAIVAEIHKIYFKFKEES"
     gene            20590..21603
                     /locus_tag="NBI57_000670"
     CDS             20590..21603
                     /locus_tag="NBI57_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HCD1821637.1"
                     /translation="MAIKYSALNLVPIREGDDERTAINDMVKLAQHLDELSYERYWIA
                     EHHNAPNLVSSATALLIQHTLEHTKHIRVGSGGIMLPNHAPLIVAEQFGTMATLFPNR
                     VDLGLGRAPGTDMMTASALRRDQHDGVYKFPEEVSLLQQYFGPAHQQAYVRAYPAVGK
                     NVPLYILGSSTDSAHLAARKGLPYVFAGHFAPQQMKEAIEIYKTLFEPSDVLDEPYVI
                     VCLNTIVAENDDEAQYLASSMAQVMVSITRGRMQPVQPPTHDLQNILTPREYAMAMER
                     QKISLIGSENTVQQKIQDFMETYGEVNEIMAISYIYDKDMQLDSYRRFKNVINQINEK
                     NTL"
BASE COUNT         6668 a         4253 c         3037 g         7856 t
ORIGIN      
        1 cttaaaatat atctaattat gctttataaa tgaatcaccg gaaatcaaaa tatatcttgc
       61 ctaatactaa caacatttac agtgatgcta ttagtcgaaa tgtcttttaa aataactgaa
      121 gtacttcttt gaatgagaat atacctgtca atattgtaac aagtactgca acgataccaa
      181 agatgaacat ccaatttggg tgtttataat caccaacaat tgatttcttt ttacttgcaa
      241 tcaaaattgc acctaacgta ataggtaaaa tccatccatt tatcgcgcct gctataatta
      301 aaaggctgat tggttttcca ataaataaga aaatcattgt tgaaataacg ataaacacaa
      361 tcacaattaa attacttctt tcgttaagtg atttatgaag tgtttttaaa aatgttgcgc
      421 ttgtgtatgc tgagccaatt actgatgaca tagctgcagc aaataacaca ataccaaaaa
      481 tattctttcc aattggtcca attgcgtgtt caaaaactga cgctggtgga ttttcagaac
      541 ttagtgtcac acctgttaca acaactccta ataccgctag gaatagtaac gttctcataa
      601 tacctgtagt taaaatacca gcaattgctg attgatttac aaacggtaaa tattgcttac
      661 ctttaatgcc agagtctaat atacgatgtg cacctgcaaa ggtaatataa ccacctacag
      721 ttccaccaac taacgtaatg atgggcaaga ctaatttcat tggatgttct ggcgcaaatg
      781 tatgcacaaa agcatcacca taaggtggat tagaaacaaa catcacatat gccacaacta
      841 aaatcatcac aataccaaga atcattgaaa caacgtccat aattttttgg ccacttttac
      901 ttacaaatat taatattgca aagattgcag taatagctgc gccccatttt acatctaatc
      961 caaaaattgc atttaaacct aaaccggcac cagcaatatt accaatatta aaagctagac
     1021 caccaaatgc aatcaatatt gagataacag taccaagccc aggaacaact ttatttgata
     1081 tttcttgacc tcttaaacca gttacaacta atatgcgcca tatattaatt tgtgcaccaa
     1141 tgtcaatgat gatagacagt aatatggcaa atgcgaaact tgcaaaaaat tgtgatgtaa
     1201 atactgctgt ttgcgttaaa aatgctggcc caattgcaga agttgccatc aaaaatacag
     1261 aacctaataa taacctttta tgattttttg tgaattcaaa gtcactttct tttttaagct
     1321 ttaaatttct ccccattttt ttagccccct ataaggattg aatatcaatg ccttctttca
     1381 ttaaaatttc tctaattttc gaaacaaata ataatgcatg ttctccatca ccatgcacac
     1441 aaattgtatc tgcttgtaac gttacttcct tattgttttt tgaaataact ttattttcct
     1501 tcaccatctt taaaacctgc ttaagtgctt cgtcagtatc agtaatcaca gcatcacttt
     1561 cttttctact aacgagctgc ccatcatctt cgtatcgtct atcagcaaac acttcagaag
     1621 ctgtaattaa tccgacattc tttgcttctg aaattagata tgaatttgct aatcctacta
     1681 acactagtga tggatcaaag tcataaacag cttgtgctat aacgtttgct atttctctgt
     1741 cttttgcacc catctgatac aatgcaccat gcggtttaac atgattaatt ttaagttgat
     1801 gaatgcgaca aaacccttgt aatgcaccta attgataaat catcaaatta taaatctcgt
     1861 cgttagagat atctatattt cgtctgccaa agcctttcaa atcaggtaaa ccaggatgtg
     1921 cacctactgc aacattatgt gctttggcaa gttttaccgt ttcattcatt acattttcat
     1981 caccagcgtg aaaaccacaa gcaacattcg cacttgtaat taacggaata atttgatgat
     2041 caccaccaaa ggaataattt ccaaatgctt cgcctaaatc acaattcaaa tcaactcgca
     2101 ttataattcc acccctttaa caatttgatg tttttctaaa aatttaatat caacatcttt
     2161 tgcatctcca tcacgatata gtggataatt taaaactgca tataaaaaat cggcagttgt
     2221 agaaaatcca tctatcacca tttcatctaa ggtgactttc aacttatcaa ttgctgaagc
     2281 tctatcatga gattttacaa ttactttagc tactaaagaa tcataatatg gtgaaacttg
     2341 ataaccgtga tatagtaaag aatcgactcg cacattaaag ccttgaggta aatgtaacgc
     2401 tgtcacttta cctggtgttg gttgaaattt cttttcagga ttttcggcat ttattctcgc
     2461 ttctatcaca tgaccattaa attgaatatc gctttgtgaa aaaggtaaat gattatgttc
     2521 caataaatac agttgtgctg caaccaaatc acgttctgct cgcatctctg taacagtatg
     2581 ttcaacttgt attcgagcat tcatttcaat aaagtaatgt gcggtatcag ttactaaaaa
     2641 ttcaatcgta cctgcacttc tataatttgc tgcacgtgca actttaacag catcgttaca
     2701 tatttgttgt cgtcgttctt cagttaatgc tgcacaagga gattcttcga ttaatttttg
     2761 atttttacgt tgtacagaac aatcacgttc ccctaaatgt acataattat cctgcccatc
     2821 tcccataact tgaacttcaa catgttttgc aacaggtata aaagcctcaa cataaacacg
     2881 atcatcatca aagtattttt ttccttcact tttagcttct ttaaatgcct tttctaaatc
     2941 ttcagctttc tttacaatac gtataccttt accaccaccg ccactggcag ctttgataac
     3001 aactggataa ccgatgtctt tggcaagatt ctcaatttca gacacatgat tcacagcacc
     3061 atttgatcct ggaatcacag gaacacctgc atgatgaact gtttgtcttg ctgttatttt
     3121 atcccccatc atttccatcg tttttttagt aggccctata aacgctatgc cttgttcctc
     3181 aacggtttga gcaaattttg ttgattctga taaaaagcca tatcctgggt gaattgcatt
     3241 agcaccagtg atttgtgcag cagatatgat gcggtcaata tttaaataac tatctaaagc
     3301 attagcttcc ccaatacata tagcttgatc tgctaaatgt acatgcaagc tttgctcgtc
     3361 cccttttgca taaactgcta cagtttcaat cccatattct ctgcaagctc ttataatcct
     3421 tacagcaatt tcacctctgt tcgcaattaa acaacgaagc atttacttac cccctttact
     3481 taatacgtac caaaacttgg tcgtattcaa catttgtgcc atgatcagct actatttcag
     3541 taatttctcc agcaacatct gttgttacct cgtttaatac tttcatcgct tcaacatatc
     3601 ctataatatc tcccttgtta actttgtcac cgacattcac aattggttca gttaattctt
     3661 tactatcttg taaaaagaat gtacctacca ttggtgattt aatgtcatga taatcatttg
     3721 tcgaaacatc ggagttatca ttcgcttttg aagctgtcaa atcattattg ttcatacttt
     3781 gatttgattg attactgtgt gcagccaaat gattcgagtc agtgaagtca atttctattt
     3841 catcttcaaa atttttatat ttaaatttct taacatcatt ttccttcact aatttgatta
     3901 tttgttcgat tttttcaata ttcattttac aaatcccctt ttaaaattgt tgataatttt
     3961 ttcgaagtat gtcgcaagct agatgtatca aaaattggag tcttttgatg actcttaaga
     4021 atttcattaa acagagacat ttgttcccga ttcttatcta cagcttcttg gaatgatatc
     4081 catttaaatt gaattgtatc ttttggtttc atctgtgcta actttggcag atcaaatttg
     4141 catacagttg caattttggt ataaccacct atcgtttgtt tatcattaag cagaataata
     4201 ggttgaccat catttggtac ttgaacacta ccaagagcaa ccggttcaga aatgatatct
     4261 gcttgattaa ttggtgcaac gctgtcacct tccaaacgat agcccatacg gtctgattgt
     4321 tcagtaatta aatatggatg atttacaatt ttcgctctag cctcttcaga aaatgcctcg
     4381 aattgaggtc cttgaagaat gtgtataata ttattttctg gcaataaatc gtcctgtaaa
     4441 tgaatagtct ttccaatgtt ttctttaaag tcattattta ttttcactgt tattacatca
     4501 ttagctaata actttctacc tttgaatcct cctatactgc ttcgggtatg tgttgcataa
     4561 ctttcagcaa tagaaggtac gttgatagaa tgaccaaaag taagataacc gcgtacacct
     4621 ttggttatag cacctatttt taaaatgtca cctttctcag ctaatatgac agaattcatt
     4681 gatatagttt tattatttag cgaagcatta atactaccac cggttataac aaatgtattt
     4741 tgcgtattaa attgaatggt aggaccaatc aaagtatatt caatcgctgg accatcattg
     4801 ttaattaatg actgcgcaac cttaaaacta aattgatcca tggcacctgc gcctgaaaat
     4861 ccaatatgtt cataacctat tcttcctaga tcttgtaccg ttgaaaagag acctggttgt
     4921 aaaatcttaa ttgacatttt caatcaccac ccagtcatca acattaaagt tgccatctga
     4981 tatatctctt tcgatttgta taaatttctg ttcatctatt gcataaaatt gtatccattc
     5041 tcctgcttcg tacattgaca ttggttcacg ctcgctgcta aatactttta acggtgtgcg
     5101 tccaataatt tgccatccgc caggagaatc tgatggatat agtcctgttt gattattcgc
     5161 aatacctaca gaacctgcat gaatttttaa ccttggctga ttacgtctag gtgtatgtag
     5221 ttgttcatca agtccgccta agtatggaaa tcctggcata aatcctagca tatatattaa
     5281 ataaggttta cttgtatgtt tttcaataac ttgctcaaca gttattcgat tatgctttgc
     5341 tacttcttca atatctggtc catatgtacc accatattga acaggtattt taataatacg
     5401 attggtttga ttcacagcat gaacattttt ttcattaaat ttgttaagtt ctaaattttc
     5461 aattaattta gaagatgtta tagcttgttc atcaaaatat attagaactg ctcgatacga
     5521 agggacaata tcttgaattt ctaatatttc tttttctcgt atccaccgta ccattgctgt
     5581 gacattacga tatgtctctt cggatatttt attttcaaaa taaatcataa ttgtctgctc
     5641 gttaataaat cttacatcca ctttaaatcc ccctttgtat tgcaataaac cagtattgaa
     5701 taccttttca ttgtatcatt gagaagcaca agttgtttaa taagtaattc aaatcgcata
     5761 caataacaaa tgataatcaa tttgttttct cggaaatatt ttgctttttt aaaatggagt
     5821 agttaatatt gtaactctta cactaattta ggttctgcta tcattcggtc tgatggaaaa
     5881 tttttacttt tcatctgtcc gattttttga ttttgaatat aaaaaagcac gaccgaagta
     5941 tcattaacac acttcaatcg cgcaattaaa caatctattt gatcatttat tggatattaa
     6001 caatttttac gttcatttcg ccaccattag gtagtggaac acgaacttca tcatctaaac
     6061 ctttaccaat taacgcttta gccattggtg attcatttga aatcttacca ttaaatgcat
     6121 ctgattcagc tgaaccaacg atttgataac tttcctcttc atcacctggt aattctacaa
     6181 acgttactgt tttaccaatt ttaacaacgt tgttatctcc agtatcttca atgattaatg
     6241 catttcttaa catatgctca attctttgaa tatcttgttc gatgaatcct tgttcatctt
     6301 ttgctgcatc atactcagag ttctctgata agtcaccaaa tgaacgtgca actttaattt
     6361 tctctacaac ttcaggacgc ttaactgttt ttaattcttc aagttcacgc tctaattttt
     6421 caaaaccttc ttgagtcatt ggatattgct tttgattttc catattgtca tcttccttta
     6481 ctgaattata ctattgcttg ctaactaaag actgaatttt tgttgtcata atatctattg
     6541 caactttatt gctcccacct tcaggaataa ttatatcagc atatttctta gtcggttcaa
     6601 taaattggtc atgcataggt ctaacaacac ttaaatattg attgataaca gagtccattg
     6661 aacgcccacg ctctttagta tctcgtgtta aacggcgtaa tattctcaag tctgcatctg
     6721 tatcaacata tattttaaca tccatcatat cacgtaatac cttattttct aaagcgaaaa
     6781 taccttctac gataataaca tctttaggtt taaaatcaat ggtaatgtca cttcttgtat
     6841 gactagcata atcatatgtc ggtacttcta ctgctttacc atttttcaag tctttaagat
     6901 tttcaattaa taaatcatta tcgaatgcaa atggatggtc ataattggtt tctaggcgct
     6961 cgtcgaaagt caagtgcttt tgatctttat aatagtaatc ttgagcaagt aaagcgacac
     7021 tatgaccttc taagtttttc ataatttcgt tagttacagt tgtttttcct gagccagatc
     7081 caccagctat gccaatgatt gtagtagctt tcattagcca atttcctttc tcatcatgtt
     7141 gtttggatat atcgggcgat ccactttaat ttgaacgatt tgtaatggat ggcgcgccgc
     7201 gtctaagctg ttaccttctt catcataaat tgcttctact acttgtgtaa atgtttcaat
     7261 ttctggacca aagaattcta tttcttgacc tggtttaaag ttatttcgtt gttgaatagt
     7321 cgcaattttt gtatcttcat tatagtctaa taccaaacca caaaaatcaa atggtgattt
     7381 tttagattgt tgttgaccaa acatctgttc ttcataacca ggtgttccct caaagaatgc
     7441 tggtgcagtg tctctatttg cacatttatc taactcaatt aaccattccg gattaatctt
     7501 aaagttgtca ggatctgccg catacgcatc aatgacttta cgatatactg agacaactgt
     7561 tgcaatataa tgaattgact tcatacgtcc ttcaattttt aatgagtcca caccaatatc
     7621 catcatttga ggaattgatt cgattaattt taaatcttta ggactcatcg caaacggtgt
     7681 aacttcacct tgattataaa atacatcaag ttcaccatta tcatcaactt ctaataattc
     7741 ataatcccaa cggcaacttt gacagcaacc gcctctgttg gaatcccttg cagtcatatg
     7801 attacttaat gtacatctac ctgaataggc gatacacata gcgccatgaa taaatgcttc
     7861 gatttcaata tctacttttt ccttcatttc acgcatttcc atcgcgccgg tctcacgtgc
     7921 taatacaaca cgatccaatc cttcttcttt ccaatattct acagctttgt aattagaaag
     7981 tgattgttga gtagataaat gaatttcaag ttttggcgca acttctttac atgtttcgat
     8041 aattaaagga tctgcaacaa tgatacctgt cgcaccagtc ttttccaaat tacgcaaata
     8101 tgattctaga ccttcaatat tctcatcatg tgcaataata tttgtcgtaa cataaatttt
     8161 ggcaccgtaa cggttcgcaa attcaacacc ttcagctatt tcttccatcg tgaaattatc
     8221 agcatttgaa cgtaatccat attcttgacc acctaaaaat acggcatcag cgccataatg
     8281 tactgctatt tttaactttt ctaagtttcc agcaggtgct aataattctg gtttcttcat
     8341 aactgtttta ggagttgatt taatctcttc tattgtcttc atgattaacc tccttaatat
     8401 actgtttgtt tatataagaa accttcgtca aatggtcgat gatcaggttg aatttcttca
     8461 attggatcaa tcaacataaa tttctcatct tcatagattt caggatcttc attgtacaaa
     8521 tctatcgctt gacgatactg ttccgttacc acattaatat attcttccgt ttgtagaata
     8581 ccatcgattt taaatgaatc tatacccgcc tcaaaaaatg gtgctaattc ttcaattaaa
     8641 caaatgtcgt ttggtgacat aatgtgcgta ccattgtaat cttcgtaaac tgggtaatta
     8701 ttttgtcttt cttcatcata aagtaataaa gattgttcat cattgcgacg ttcaattttc
     8761 atttggcgat cttggaacgt ataataattg cctagtagca tacgctttga ttggaacata
     8821 caagtcattc cttgaacttg cacctcaatt tccacatttg aattttcttt tatattaata
     8881 atttcatcca aattcagctc acgtgctaag acagctcttg atgcgcctct tttaccccag
     8941 taattacatt gaaaatgatt agttactaac gtctctgcat tccaatgaag tggtattgga
     9001 ttttcttgcg ccttcacata cattactact gctggatccc cgaaaataat tctgtcaact
     9061 cgtatttcat gtaaaaaatt aatataatct tctacagcat ctaaatgata attatgaaat
     9121 aatccattca ctgccgcata tactttttta tcgtttttgt gagctaatgc gacagcctct
     9181 gtcatttgtt gtctattgaa ttcccctgga agtcttaaac caaacttttg ctcgccaatt
     9241 acaaaagcat ctgcacctaa atcaataagt gtttccatat ggcttaatga cttgggtgtg
     9301 acaagtaatt ctgtcatagt cattctcctt taattgaaat cgctaatcca tcgtctatat
     9361 ttaaaaaatt cgttgtatat cctggttgct ttattaacca ctcattataa tcttgaacct
     9421 ttttaaccat ttgtcttaca tttctcgatc taacaatccc aatatccgat acaaaaccgt
     9481 gatataaaac attatctgta attacgagac cttggtgctt taaaagtggt gtatatattt
     9541 caaaaaattt ctttgattgc gcttttgctg catcaataaa tatcatatca taaactttgt
     9601 catttacatt ttcaaattgc tctaaagcat taccttcaat aattcgaacc tggttttcaa
     9661 aatgataagt agctaaattt tgtttagcat attgaatcat cgtttcatta cgctctatcg
     9721 ttgtgacatg aatgtcatca gatatagaag cgaattgcat agaactatag ccgattgctg
     9781 taccaatttc taaaatattt ttaacattat tcatacgaat taattgctta attaaatcta
     9841 atgttaaacg atctacaatt ggcacttcat ttccctcggc aaattcacgc aaaacttcga
     9901 ttgaactatt ttgatgttga tgtaaatcta ttaaatattt tttatttagg tcatccatgt
     9961 tttaaacttc ctttatgtaa aataagtcaa tatgattatg acaataaaat aaatcagcct
    10021 tcacaattga ttataatttt gccaaccaat taaatgactg atttcttgtt agacgcaaag
    10081 ctattttatt tatagaagcg aatcattcat ataaaattta actttagata ttttaccata
    10141 ttttcaataa aattataagc gttaattatt tatacattgc ttgacttaaa aaaatactct
    10201 tgcctaccca tcttaaggtt agcaagagta aaatcttttt aattattctt ccatttcagt
    10261 atttacaact tcttcaatca tgtcccattc ttcatcagtt tcgattggta ctaacttacc
    10321 accgtcacct gactcatctg gttcattgat cattggtaca agctcaatca tatcgtcttc
    10381 atctgattga gcaccttctt cagctaagat aacatactct tttttgaatt caggatgata
    10441 aaattctaaa acttttcggt ataaaacttc atttccctct tcatcgaata aagttaataa
    10501 ttcttcttcg ttattaattt ctagttgtga atcatgatta tgttcagtca tagtaaaatc
    10561 tccttttaat gtagtgaatc taaatagcct tgtaaaataa ataccgctgc cattttatca
    10621 atcacttgtt ttcttttttg tcttgaaaca tctgcttcta ataatgatcg ttcagcagcc
    10681 attgtgctta atctttcatc ccacatcaca atctcaatag aaggataagc ttctaataat
    10741 ttttctttat atgttaacga agcttcgcct cgaaatccta ttgaattatt catgttttta
    10801 ggtagtccta ttacgactgt acccacatta tgttttttaa taatgtctac taattggtca
    10861 atacctaatt cattattttc ttcattgatt cggagtgtgt ctaatccttg tgccgtccaa
    10921 cccattatat cactaattgc aattcctacc gttctactac cgacatcgag tcctaaaatt
    10981 ttatgttgta acataaatta tttattttgc tcttttaaat agtaagaaac aagttcttcc
    11041 ataataacat ctctatcaat atgacgaatt tgatttcttg cttcattctg gcgtggaata
    11101 tacgcagggt cacctgataa taaataacct acaatttggt ttacggcatt atatcctcgt
    11161 tcatctaatg ttcgataaac attatttaaa acatctctta catcttgcgt tggaagttct
    11221 tcatagtcga atttcattgt tttatcaaag ttttccattt gcgacactcc tttaattaca
    11281 aatataactc actatcatca tacaatatta tggctttaaa ttatagattt ttaatgtaat
    11341 ctttaataaa gcttaatgat tttgagatat tttcaggttg tgtaccgcca ccttgagcca
    11401 tatctggacg accgccacct ttaccaccaa cgattggtgc catttgtttg ataagatcac
    11461 cggctttaac gttatttgtt aaagatttag ggacagttgc aaccatcgat actttatcat
    11521 caacattact tgcaagaatg ataattgtat cttgtagttt agatttaaaa tcatccattg
    11581 tcgagcgaat tgctttcgca tttggtacat ccacttcagt aaccaatact ttatagccat
    11641 tgatttcttc aacttgatct tcaatattat ccattttaag tgatgtgatt tctttgtcac
    11701 gttgctctaa ttgttttaat aatgcttttt cttcatcttg taattgtgtt aacttttcga
    11761 ctacttgatc atcagatttc actttcatct gtgatttcat cgtattaaat ttctcttgaa
    11821 tatcttctaa atataagaaa gctgctttac ctgttaatgc ttcaatacga cgcacaccag
    11881 ctcctgtacc tgactcactt actattttga ataagccaat ttcagaagta ttgcggacat
    11941 gaataccacc acataattca attgaaaatg gtgccatatt tactacacgc acaacatcac
    12001 catatttttc accgaataat gccattgcgc ccatttcttt agctgaagca atatccattt
    12061 cttgaatgtt aacgtcaata cctttccaaa tttcttcatt tactaagcgt tcaacttgat
    12121 caatttcatc attagtcatt ggaccaaaat gagagaaatc aaaacgtaaa cgatctgctt
    12181 ctactagtga accagcttgg ttaacatgat cacccagtac tgatttcaac gctgcatgta
    12241 ataaatgtgt tgcactatgg ttcttttgaa tgtcacgtcg atcattttgg ttcacttcag
    12301 cagacactgt agcgccaaca tttacttggc caaattgtac tactccttta tgcaagtttt
    12361 gaccatttgg tgctttggtt acttcactaa cagcaatttc aaaattgtca ttataaacaa
    12421 tacctgtatc cgcaacttgt ccaccactga ctgcataaaa tggtgtttcc gttaacatga
    12481 agtatactgt ttcacccgct tcaacttgtg aaacttcttc accattatat atcaagtgtg
    12541 ttagtgttgt ttgagctgtc gcagtatcat aaccaacaaa agtacttgca gatgtaatat
    12601 ttttcaatac ttcactttga acttgcattg attgagaatt ttgacgtgct tgacgtgcac
    12661 gatcacgttg ttgttgcatt tctgactcaa atgttgtcat atcaactttc aatcctgctt
    12721 gcactgctat ttcttcagtt aattcaattg ggaacccgta cgtatcatac aatttaaatg
    12781 catctttccc attaatttca tttgttgtcg ctttagcttt tttaattaat tcatttaaaa
    12841 tcgctaaacc atcttctaat gtttcatgga atcgttcttc ttcagacttt ataacacgct
    12901 taatgaaatc tgctttttcc ttaacatttg gataatatgg ttccataatg tctgcaacaa
    12961 tatcaacaag tttgtacata aatggctcat tgattcctaa cgtttgacta aaacgaacgg
    13021 cacgacgtaa caatcgacgt aatacatatc ctctaccttc attggcaggt aatgcaccat
    13081 cagaaattgc aaatgcaatc gtacgaatgt ggtcagcaat tactttaaat gccacatctt
    13141 gttcgttgtt tactaaatat tgtttacctg atactttttc gatttcattc attataggca
    13201 taaataaatc tgtttcatag ttagtacgta cattttgaga aactgaggcc atacgctcaa
    13261 gccccatgcc ggtatcaata tttttattag gtaatggtgt gtaactatga tctttattat
    13321 gattgaattc actaaatact aagttccata cttcaagata gcgttcattt tctccacctg
    13381 gatacatttc ttctgccgga tcgtcttgtc catatgcttc tccgcgatca tagaaaatct
    13441 cagtgttcgg tcctgaaggc ccttcaccaa tatcccagaa gttaccttca atgcgaataa
    13501 tacgactttc ttcaagccca atatctttat gccaaatgtt atatgcttcc atatcttccg
    13561 gatgaatcgt aacgtacaat ttatctggct ccatacccat ccatttatca ctcgttaaaa
    13621 attcccaagc aaattcaatc gcttcttgtt taaaataatc accaattgag aagttaccta
    13681 acatttcaaa gaatgtatgg tgacgcgctg tgaaaccaac attttcaata tcatttgtac
    13741 gaatagcttt ttgagagttt acaattcttg gctttttagg tgtttcacgt ccatcaaaat
    13801 atttctttaa tgttgctaca cctgaattaa tccataataa tgtatcatca tcaattggca
    13861 ctaatggtgc agaaggttca accatatgtc ctttttcaac aaagaaatct agatattttt
    13921 gtctaatttc actcgctttt aactttttca tcttttacac atcctattta ctgtttttaa
    13981 attaccattc cataaaaatt gatgacacag atagtcgatt tgaaaaacta gtataaatca
    14041 atatcatttt ttattattaa aaaataaaaa acgcccatcc tcaaaaggga cgaacgttat
    14101 cgcggtacca ccctagttat aaatgcaatt caacacattt atcactttaa atcgactata
    14161 cagttgtgca taaagtagcg ttcactaatg tttgttgtac ttttcaccaa ccagtacatc
    14221 tctgataaac aaatcattaa ctactcatct ttatacgaat ttaattctat tttagttaca
    14281 tttacgcttg ttgtcaacgt tctataaagt catacggcgt gatttctccc atattaatca
    14341 ttgggtcaat tttaaacatt gtagcttccg ttaatacatt tatatctgtt tttgttgaat
    14401 cagacgtaac ttctccacta tcattcgatg acattggcgc ttctacttga tcatctattg
    14461 tcgtttgtga agctcctgta tcattagttg ctgtgttttc cagcatttct tcatcttctg
    14521 aattaaaata atttttcaac aatgtacata attgtgttaa acgcgcttga ccatttgttt
    14581 ttaatccaat atcaaatgct tctggatcac caagtagaac taaactcgtt ttcgctctag
    14641 ttaaaccagt atataatatc ggtctttgta acattctaaa atactgttta acaataggca
    14701 tgataacaat aggaaattct gaaccttgtg atttatggat tgatgtacaa taagcatgtg
    14761 ttaattccat catatcttgt ttcgtaaatg taatttcatt accttcaaaa tccacaacaa
    14821 gtacatcttt attaagggca ttttctttcg cccaaaaaat accaacaata actcctatgt
    14881 caccattgaa tatgttatca tttggcctat taacaagttg taatactttg tcaccttttc
    14941 taaagactac atcaccaaac tcaatttctc gtgtgtcttt ctttttaggg tttaaaatat
    15001 cttgtaaaac ttgatttaaa cgtttaatac cggcatttcc tttatacatt ggtgcaagca
    15061 cttgaatatc agccatagta taccctttat taacagcact agtaactacc ttctcaacaa
    15121 ctgttggtat ttggtttgcc tgacagttaa taaaacttct atcatgaaaa cgctgtgtaa
    15181 tatcaatttt ctgacccaat ttcattcgat gtgctaattc tataatgctt gaaccatctt
    15241 gttgacgata tacttcagtc agattaactc gtggtatagc tttcgattca attaaatctt
    15301 taaatacttg accaggacct acagaaggca attggtcctc atcacctaca aatatcaatt
    15361 gtgcatctaa aggaactgca cttaaaaatt ggtgaaacaa ccaagtatct accatagaca
    15421 tctcatcaat gattatgagt cgtgcgttta tttcattttc taatatatcc tctggctttg
    15481 tgtcttgatt ccaacctatt aaacgatgaa tcgtcattgc ttctaatcca gttgactctt
    15541 gtagtctctt agacgctctt cctgttggcg ctgctaatac aactggataa tcatcattga
    15601 cataatcatc ataatctaat gataagccat gaatctcagc atataattct acaatacctt
    15661 taattactgt cgtctttcct gttcccggtc caccggttaa tagcatcacc ttagaattga
    15721 tagccgtttg caaagcttct ttttgtgaag ctgcatagtt cacttgattc gcatcttcta
    15781 tttcaccaat atgcatttgt aaatctgact gttcaatttc cgtaagttta tttgtatgcg
    15841 tctttattct gaataagttt tgaacacttt tgatttcaga ataatacaaa cttggaattg
    15901 caacttgttc attgtcaata attagtcgtt tttcctcatt taagtattgc aacatttcgt
    15961 ctaatttttc aggttcgatg acctcttcat cttgataatt taatacatca accgttaaat
    16021 ctataacaac attgataggc aaatatgtat gtccctgttt aatacattct tcttctaacg
    16081 tatagagcaa cgcagctttt aatcgttcat tatcgttata agcgatacca atatttctag
    16141 caagttgatc tgctttatta aaaccaatac ctttaatatc ataaatcaat tgatatggat
    16201 ttcgatctaa aatagtcagt gtatcgccga gataaaactg ataaattgcc attgaaagtt
    16261 taggaccaaa ccctaaatca tgtaaacgaa tcattatttt ttcagattct tgatttgctg
    16321 aaatttgttc tgcaatttgt ttctgtttct ttttagataa tcccgaaact ttttctagca
    16381 ctgaatggtc atctaatata tcatttatcg cattgtcacc taatgtatta acaatatttt
    16441 gagctgtctt tttacctaca cctttaaaca aatcactaga taaataactt ataattgctt
    16501 ctttcgtttg tggcatttct ttttcaaaag tctctgcttt taattgttta ccataacgtg
    16561 gatgatcaac aacttgccct ttaaatgtgt agacatcgcc ttcaacaata ttcggaagaa
    16621 accctacaac agttggcatt gtatcaaagt cttcatttgt ttcaatagta tctactttaa
    16681 gcactgtata aaaattatca ctgttttgaa acaatatcgc ttcaacagta cctttgatca
    16741 ttgaataatc aaatagtgta gggtctgaca tgttactcct cctctttcat tttagtgaat
    16801 gttttcagcg catgctgact taataagtgt ttagggtcga tagtcacagc ttctttaaaa
    16861 tgagttattg cttcatcaat atcttcattt ttcataaata acgctaagcc caaattgtat
    16921 cttgcatcaa catgattttt atcaatcgtt aatacatgtt taagttgagt tatggcttca
    16981 ttaaacattt ctaattgaca taatacaaga ccatattgaa attgaacttc tgcatctttg
    17041 tctttatcta gttccgcagc agtcattaaa tacggcaatg ccagcttaaa tgattctaac
    17101 tgattaaacg ccataccgat catataatta caatcaactt gttcaatctc tgtttgtaat
    17161 gcttgttgat ataatttaat agcttcttga taacgttgct gattataata tacatttgct
    17221 agattataaa atacgacgcc attcttcgga tctattgtta aagctttttg gaaaaaacgc
    17281 tctgcctttt caatctcatt cgcatcagca agtacgatac cagcattaat ataattttca
    17341 ataattgtag gatcttcttc gatatttccg aacaatgctt gtaacgcttc ttctattttt
    17401 ccattttgta tgtattgata aattgtttgt tgatctatca tttacgaacc tcatttctca
    17461 tcaattataa catcttgata aattgtatgt ctcgaatcac ttaacaacga ataaaatata
    17521 atctaatatc attttcattc atgaaaaagc gggaatggaa tagaaatgct aaagaaccat
    17581 taacggttta ttatgtaatg gttcttccac attagccacc actattatgt acttaaaaat
    17641 aggaatacat aattagattc atgcataggg agtgggacag aaatgatatt ttaacaaaat
    17701 taaattcgtt atccccaact ggcattgcct gtagaatttc tttacgaaat tctctgtgtt
    17761 ggggccccgc caacttccat tgcctgttga aattggggtt ccaatttctc tatgttgggg
    17821 ccccgtcccc aacttccatt gcctgtagaa tttctttacg aaattctcta tgttggggcc
    17881 ccgccaatat aacattgtag agcctaggac attgtgatgt cccagactct atcatcatga
    17941 attattctca tcaaaaactg tctttcgtca ttttcgacgt tgaaacttca aataagtaat
    18001 ttattgttgc cattgtttat acaacataat ttaattgacc ttcatttttg aacacatcgt
    18061 caattgttgc accaccaaga cacacatcac cttgataaaa aacaactgct tgtccaggtg
    18121 tgattgctct tactggctca gcaaaagtaa cacgtagtgc atggtcgttt tcacgtttca
    18181 caaaaacttt cgtatctttt tggcgatatc taaatttagc tgtacattca aaaccttgat
    18241 ctaagtcatt atcttctgga tttacaaatg aatagtctga agcaattaag taatcactgt
    18301 ataatgcatc gtgatggaat ccttgttcta catataaaac attatctttt aggtttttac
    18361 cgacaacaaa ccaaggatcg ccatctccac ctatacctaa tccatgtctt tgtcctattg
    18421 tgtaatacat caaaccacta tgtttaccca ttttcttacc atcaagtgtt atcatatcac
    18481 ccggttgtgc aggtaaatat tgtgataaaa atgttttaaa gtttttttcg ccgataaaac
    18541 aaatgcctgt agaatctttt ttcttagcag taacaagtcc ttgttcttca gcaattcgac
    18601 gcacttcact cttttcgatg tcgccaattg ggaacatcac ttttgaaagt tgttgttgag
    18661 ataattgatt caagaagtat gtttgatctt tattattatc tacaccacgt aacatttcaa
    18721 catgaccatc ttcatgacga tgtatgcgtg cgtaatgtcc tgttgctaca taatctgcac
    18781 ctaaattcat cgcatgatct aaaaaggctt taaacttaat ttctttatta cacataacgt
    18841 ctggatttgg agtacgacct tttttgtatt catctaagaa atacgtaaag actttatccc
    18901 aatattcttt ttcaaaatta acagcgtaat atggaatgcc aatttgatta cacacttcaa
    18961 taacatcgtt gtaatcttca gttgcagtac atacgccatt ttcgtcagtg tcatcccagt
    19021 ttttcataaa tatgccaatg acatcataac cttgttcttt taagacgtgg gctgttacag
    19081 aactatctac accgcctgac ataccaacga caacacgtat atctttattt gacaattatg
    19141 actcctcctt aaatttaaaa tatattttat gaatttcagc tacaattgca ttaatttcat
    19201 tttcagtagt caattcgtta aaactaaatc gaatcgaatg atttgatcgc tcctcatctt
    19261 cgaacattgc atctaaaaca tgcgacggtt gtgtagagcc tgctgtacat gcagatccag
    19321 acgacacata gatttgtgcc atatccaaca atgttaacat catttcaact tcaacaaacg
    19381 gaaaatatag atttacaata tggcctgtag catccgtcat tgaaccattt aattcaaatg
    19441 gaatcgctct ttcttgtaat ttaactaaaa attgttcttt taaattcatt aaatgaatat
    19501 tgttatcgtc tcgattcttt tctgctaatt gtaatgcttt agccatcccg acaatttgtg
    19561 caagattttc agtacctgca cggcgtttca attcttgttc accgccaagt tgaggataat
    19621 ctaatgtgac atggtcttta actaataatg caccgacacc ttttggtccg ccaaacttat
    19681 gtgcagtaat actcattgcg tcgatctcaa attcgtcaaa cttaacatca agatgtccaa
    19741 ttgcttgaac cgcatcaaca tggaaatatg catttgtctc agcaataata tcttgaatat
    19801 cataaatttg ttgcactgtg ccaacttcat tatttacaaa cataatagat actaaaatcg
    19861 tcttatctgt aattgtttct tcaagttgat ctaaatcaat agcacctgta tcatcaacat
    19921 ctagatatgt tacatcaaaa ccttctcgct ctaattgttc aaaaacatgt aacacagaat
    19981 gatgttcaat cttcgatgtg ataatgtgat tacccaattg ttcatttgct tttactatgc
    20041 ctttaattgc cgtattattc gattctgttg caccactcgt aaatataatt tcatgtgtat
    20101 ctgcaccaag taattgtgca atttgacgtc ttgactcatc taaatattta cgcgcatctc
    20161 ttcccttagc atgtattgat gatggattac cataatgcga attgtaaatc atcatcatcg
    20221 catctactac ttcaggtttt actggtgtgg tcgcagcata atctgcataa atttccatgt
    20281 ttggacactc ctcacaattt tatcaatgtt ccaataatag caccttacat actatttttc
    20341 tacttttctg tttaacttta tttataatgt ttttaattat attttaccat tttctacaca
    20401 tgcttttcga taggcttttt taagtttatc gctttattct tgtctttttt ataaatttta
    20461 gtatttgcag atattttttc atttgtaaaa tgtaacgtac tattattttg gttatgagca
    20521 atttaatatt tatctggtta ttcgattggt atacttctta tatcataaaa aaggaaggac
    20581 gatataaaaa tggcgattaa atattcagca ttaaaccttg tccctattcg agaaggtgac
    20641 gatgaacgaa cagcaattaa tgatatggtt aagctcgcac aacatttaga tgaattatcg
    20701 tatgaaagat attggattgc tgaacaccat aacgctccca acctagtaag ttcagcaact
    20761 gctttattga ttcaacatac gttagaacat acgaaacaca tacgtgtagg ttctggagga
    20821 atcatgttac ctaaccatgc tccattaatc gttgcggaac aattcggcac gatggcaaca
    20881 ttatttccaa atcgtgtcga tttaggatta ggacgtgcac ctggaacaga tatgatgacc
    20941 gcaagtgcat taagacgaga tcaacatgat ggtgtttata aatttccaga agaagtttca
    21001 ttattacaac aatatttcgg cccagctcac caacaagcat atgttcgtgc ttatccagca
    21061 gtaggtaaaa atgtgccttt atacattctt ggttcttcaa cagattctgc acatttagct
    21121 gctcgcaaag ggcttccata tgtgttcgct ggacattttg cacctcaaca aatgaaagaa
    21181 gctatcgaaa tttacaaaac gttatttgaa ccttctgatg tattagacga accttatgtt
    21241 attgtatgtt taaatacaat cgttgctgaa aatgatgacg aagcacaata tttagcttca
    21301 tctatggcac aagtaatggt tagtatcact cgtggcagaa tgcagcccgt tcaaccgcca
    21361 acacatgatc tacaaaatat attaacgccg agagaatatg cgatggctat ggaaagacag
    21421 aaaatatcat taataggttc agaaaatact gttcaacaaa aaattcaaga ttttatggaa
    21481 acttatggtg aagtcaacga aattatggca ataagttata tttatgataa agatatgcaa
    21541 ttagactctt atcgtcggtt caagaatgtt ataaatcaga taaatgaaaa aaacacttta
    21601 taatgtgata aataaactaa gtgaaagtat gtatccataa tattaataaa aataaacagt
    21661 aacagcattt tgaataaaag atgtctttat tgttcaatca tttgttttag taatgattca
    21721 aattcactta aaattctaaa gcaaatatga aagcgcccct tcaactttac actgtgttag
    21781 tgtttatttg atggggcgct ttcaaaatat tgaa
//
LOCUS       DAHHSC010000022        21780 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.022, whole genome shotgun sequence.
ACCESSION   DAHHSC010000022 DAHHSC010000000
VERSION     DAHHSC010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 21780)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21780)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21780
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.022"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            complement(201..899)
                     /locus_tag="NBI57_000671"
     CDS             complement(201..899)
                     /locus_tag="NBI57_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superantigen-like protein SSL9"
                     /protein_id="HCD1821638.1"
                     /translation="MKLTALAKATLALGILTTGVFTAESKAVHAKVELDETQRKYYIN
                     MLHQYYSEESFESTNISVKSEDYYGSNVLNFNQRNKTFKVFLLGDDKNKYKEKTHGLD
                     VFAVPELIDIKGGIYSVGGITKKNVRSVFGFVSNPSLQVKKVDAKHGFSINELFFIQK
                     EEVSLKELDFKIRKMLVEKYRLYKGASDKGRIVINMKDEKKYVIDLSEKLSFDRMFDV
                     MDSKQIKNIEVNLN"
     gene            complement(1276..1974)
                     /locus_tag="NBI57_000672"
     CDS             complement(1276..1974)
                     /locus_tag="NBI57_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superantigen-like protein SSL8"
                     /protein_id="HCD1821639.1"
                     /translation="MKFTAIAKAIFVLGILTTSVMITENQSVNAKGKYEKMNRLYDTN
                     KLHQYYSGPSYELTNVSGQSQGYYDSNVLLFNQQNQKFQVFLLGKDENKYKEKTHGLD
                     VFAVPELVDLDGRIFSVSGVTKKNVKSIFESLRTPNLLVKKIDDKDGFSIDEFFFIQK
                     EEVSLKELDFKIRKLLIKKYKLYEGSADKGRIVINMKDENKYEIDLSDKLDFERMADV
                     INSEQIKNIEVNLK"
     gene            complement(2313..3008)
                     /locus_tag="NBI57_000673"
     CDS             complement(2313..3008)
                     /locus_tag="NBI57_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superantigen-like protein SSL7"
                     /protein_id="HCD1821640.1"
                     /translation="MKLKTLAKATLALGLLTTGVITSEGQAVHAKEKQERVQHLYDIK
                     DLYRYYSSESFEFSNISGKVENYNGSNVVRFNQEKQNHQLFLLGKDKDKYKKGLEGQN
                     VFVVKELIDPNGRLSTVGGVTKKNNKSSETNTHLFVNKVYGGNLDASIDSFLINKEEV
                     SLKELDFKIRKQLVEKYGLYKGTTKYGKITINLKDEKKEVIDLGDKLQFERMGDVLNS
                     KDIQNIAVTINQI"
     gene            complement(3454..4158)
                     /locus_tag="NBI57_000674"
     CDS             complement(3454..4158)
                     /locus_tag="NBI57_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superantigen-like protein SSL5"
                     /protein_id="HCD1821641.1"
                     /translation="MKMAAIAKASLALGILATGTITSLHQTVNASEHEAKYENVTKDI
                     FDLRDYYSGASKELKNVTGYRYSKGGKHYLIFDKNRKFTRVQIFGKDIERFKARKNPG
                     LDIFVVKEAENRNGTVFSYGGVTKKNQDAYYDYINAPRFQIKRDEGDGIATYGRVHYI
                     YKEEISLKELDFKLRQYLIQNFDLYKKFPKDSKIKVIMKDGGYYTFELNKKLQTNRMS
                     DVIDGRNIEKIEANIR"
     gene            complement(4522..5401)
                     /locus_tag="NBI57_000675"
                     /pseudo
     CDS             complement(4522..5401)
                     /locus_tag="NBI57_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498975.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="superantigen-like protein SSL4"
     gene            complement(5510..5647)
                     /locus_tag="NBI57_000676"
     CDS             complement(5510..5647)
                     /locus_tag="NBI57_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1821642.1"
                     /translation="MFVKVAFLCLKSDESSNVPSVESHQNQFYLTNIMDFLIYLTMIQ
                     I"
     gene            complement(5765..6835)
                     /locus_tag="NBI57_000677"
     CDS             complement(5765..6835)
                     /locus_tag="NBI57_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superantigen-like protein SSL3"
                     /protein_id="HCD1821643.1"
                     /translation="MNMKTIAKTSLALGLLTTGAITVTTQSVKAEKIQSTKVDKVPTL
                     KAERLAMINITAGANSATTQAANTRQERTPKLEKAPNTNEEKTSASKIEKISQPKQEE
                     QKSLNISATPAPKQEQSQTTTESTTPKTKVTTPPSTNTPQPMQSTKSDTPQSPTIKQA
                     QTDMTPKYEDLRAYYTKPSFEFEKQFGFLLKPWTTVRFMNVIPNRFIYKIALVGKDEK
                     KYKDGPYDNIDVFIVLEDNKYQLKKYSVGGITKTNSKKVDHKAELSVTKKDNQGMISR
                     DVSEYMITKEEISLKELDFKLRKQLIEKHNLYGNMGSGTIVIKMKNGGKYTFELHKKL
                     QEHRMADVIEGTNIDKIEVNIK"
     gene            complement(7126..7821)
                     /locus_tag="NBI57_000678"
     CDS             complement(7126..7821)
                     /locus_tag="NBI57_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superantigen-like protein SSL2"
                     /protein_id="HCD1821644.1"
                     /translation="MKMKNIAKISLLLGILATGVNTTTEKPVHAEKKPIVISENSKKL
                     KAYYTQPSIEYKNVTGYISFIQPSIKFMNIIDGNSVNNLALIGKDKQHYHTGVHRNLN
                     IFYVNEDKRFEGAKYSIGGITSANDKAVDLIAEARVIKADHIGEYDYDFFPFKIDKEA
                     MSLKEIDFKLRKYLIDNYGLYGEMSTGKITVKKKYYGKYTFELDKKLQEDRMSDVINV
                     TDIDRIEIKVRKA"
     gene            complement(8107..8787)
                     /locus_tag="NBI57_000679"
     CDS             complement(8107..8787)
                     /locus_tag="NBI57_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superantigen-like protein SSL1"
                     /protein_id="HCD1821645.1"
                     /translation="MKFKAIAKASLALGMLATGVITSNVQSVQAKTEVKQQSESELKH
                     YYNKPVLERKNVTGYKYTEKGKDYIDVIVDNQYSQISLVGSDKDKFKDGDNSNIDVFI
                     LREGDSRQATNYSIGGVTKTNSQPFIDYIHTPILEIKKGKEEPQSSLYQIYKEDISLK
                     ELDYRLRERAIKQHGLYSNGLKQGQITITMKDGKSHTIDLSQKLEKERMGDSIDGRQI
                     QKILVEMK"
     gene            9259..10104
                     /locus_tag="NBI57_000680"
     CDS             9259..10104
                     /locus_tag="NBI57_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCD1821646.1"
                     /translation="MNIMLTGATGHLGTHITNQAIANHIDHFHIGVRNVEKVPDDWRG
                     KVSVRQLDYFNQESMVEAFKGMDTVVFIPSIIHPSFKRIPEVENLVYAAKQSGVAHII
                     FIGYYADQHNNPFHMSPYFGYASRLLSTSGIDYTYVRMAMYMDPLKPYLPELMNMHKL
                     IYPAGDGRINYITRNDIARGVIAIIKNPDTWGKRYLLSGYSYDMKELAAILSEASGTE
                     IKYEPVSLETFAEMYDEPKGFGALLASMYHAGARGLLDQESNDFKQLVNDQPQTLQSF
                     LQENI"
     gene            10123..10482
                     /locus_tag="NBI57_000681"
     CDS             10123..10482
                     /locus_tag="NBI57_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 domain-containing protein"
                     /protein_id="HCD1821647.1"
                     /translation="MNIISTILIIFVALEFFYIMYLETIATTSKKTSETFNISVDKLK
                     DKNINLLLKNQGVYNGLIGVLLIYGLFISSNPKEICTAILVYIIGVAIYGGLSSNISI
                     FFKQGTLPVLALISMLW"
     gene            11014..11166
                     /locus_tag="NBI57_000682"
     CDS             11014..11166
                     /locus_tag="NBI57_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008530238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1821648.1"
                     /translation="MVPEEKGSITLSKEAAIIFATAKFKPFKNRIKNNPLKTNPFLKL
                     HENKKS"
     gene            11427..11699
                     /locus_tag="NBI57_000683"
     CDS             11427..11699
                     /locus_tag="NBI57_000683"
                     /inference="COORDINATES: protein motif:HMM:NF013677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCD1821649.1"
                     /translation="MTKERRTFSSEFKLQMVRLYKNGKLKNEIIRKYDLKPSIISNSI
                     KQHQNTGPFNHQDNLKSDEKELIKLRKEVQHLKMEHDVLKQILKLI"
     gene            12098..12802
                     /locus_tag="NBI57_000684"
     CDS             12098..12802
                     /locus_tag="NBI57_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HCD1821650.1"
                     /translation="MSKNINQANSKLNTSNKKLKQAYSKSDSKNMKVIYMPHWLEFYS
                     IAIHSDVTSNKKRYYKSFSRGTVVYVKLGSNIGSEFSGNHFCVILDNKDNKGKETVTI
                     VPLSSKGNKNYLKLNESVLNLTATDLKKQIIDISSKVQALTQKYRDIDIKLSTEDKKL
                     LSNLNQKANELYRVIGVYSKHKGKDTYVNISAITTISKRRISKINDSDPTGTIIISED
                     DMNEIEKQLKIKFFSN"
     gene            13194..13730
                     /locus_tag="NBI57_000685"
     CDS             13194..13730
                     /locus_tag="NBI57_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1821651.1"
                     /translation="MHNKNEFNKKLKDFIGSDKRMALVKGYINEYKLTTVLRALNNSD
                     YTKGTIHTRSIGNLKQIVDNRIIPKNITQNKIFKLSNLTLEVNLYERNTIYHGDFAIY
                     YPVESALIIETDTPRLINHINNNPVQKIFIITTNNWSFNTLELEKIVDETIIYDLKQE
                     DPKQYEILYKNKHGKLPY"
     gene            complement(13843..15384)
                     /gene="guaA"
                     /locus_tag="NBI57_000686"
     CDS             complement(13843..15384)
                     /gene="guaA"
                     /locus_tag="NBI57_000686"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019166941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HCD1821652.1"
                     /translation="MEMAKEQELILVLDFGSQYNQLITRRIREMGVYSELHDHEISIE
                     EIKKMNPKGIILSGGPNSVYEEGSFTIDPEIYNLGIPVLGICYGMQLTTKLLGGKVER
                     ANEREYGKAIINAKSDELFAGLPAEQTVWMSHSDKVIEIPEGFEVIADSPSTDYAAIE
                     DKKRRIYGVQFHPEVRHTEYGNDLLNNFVRRVCDCKGQWTMENFIEIEIEKIRQRVGD
                     RRVLCAMSGGVDSSVVAVLLHKAIGDQLTCIFVDHGLLRKGEGDMVMEQFGEGFNMNI
                     IRVNAKDRFMNKLKGVSDPEQKRKIIGNEFVYVFDDEASKLKGVDFLAQGTLYTDVIE
                     SGTKTAQTIKSHHNVGGLPEDMEFELIEPINTLFKDEVRKLGIELGIPEHLVWRQPFP
                     GPGLGIRVLGEITEDKLEIVRESDAILRQVIREEGLEREIWQYFTVLPNIQSVGVMGD
                     YRTYDHTVGIRAVTSIDGMTSDFARIDWEVLQKISSRIVNEVDHVNRVVYDITSKPPS
                     TIEWE"
     gene            complement(15409..16875)
                     /gene="guaB"
                     /locus_tag="NBI57_000687"
     CDS             complement(15409..16875)
                     /gene="guaB"
                     /locus_tag="NBI57_000687"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002955225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HCD1821653.1"
                     /translation="MWESKFAKESLTFDDVLLIPAQSDILPKDVDLSVQLSDKVKLNI
                     PVISAGMDTVTESKMAIAMARQGGLGVIHKNMGVEEQADEVQKVKRSENGVISNPFFL
                     TPEESVYEAEALMGKYRISGVPIVDNKEDRNLVGILTNRDLRFIEDFSIKIVDVMTQE
                     NLITAPVNTTLEEAEKILQKHKIEKLPLVKDGRLEGLITIKDIEKVIEFPNAAKDEHG
                     RLLVAAAIGISKDTDIRAQKLVEAGVDVLVIDTAHGHSKGVIDQVKHIKKTYPEITLV
                     AGNVATAEATKDLFEAGADIVKVGIGPGSICTTRVVAGVGVPQITAIYDCATEARKHG
                     KAIIADGGIKFSGDIIKALAAGGHAVMLGSLLAGTEESPGATEIFQGRQYKVYRGMGS
                     LGAMEKGSNDRYFQEDKAPKKFVPEGIEGRTAYKGALQDTIYQLMGGVRAGMGYTGSH
                     DLRELREEAQFTRMGPAGLAESHPHNIQITKESPNYSF"
     gene            complement(16913..18181)
                     /locus_tag="NBI57_000688"
     CDS             complement(16913..18181)
                     /locus_tag="NBI57_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000793018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HCD1821654.1"
                     /translation="MKNLILSVQHLLAMYAGAILVPIIVGTSLKFTPEQIAYLVTVDI
                     FMCGVATFLQANKVTGTGLPIVLGCTFTAVAPMILIGQTKGIDVLYGSLFLSGILVII
                     IAPFFSHLVKFFPPVVTGSVVTIIGINLMPVAMNYLAGGQGAKDYGDVKNILLGLMTL
                     IIILVLQRFTTGFIKSIAILIGLVLGTIGAGLLGMVDINQVNHAGWLGIPVPFRFSGF
                     SFDVTSTLVFFIVAIVSLIESTGVYHALSEITGKKLERKDFRKGYTAEGLAIVLGSIF
                     NSFPYTAYSQNVGLVSLSGAKKNNVIYGMVVLLLICGCIPKLGALANIIPLPVLGGAM
                     IAMFGMVMAYGVSILGHIDFKNQNNLLIIAVSVGLGTGISAVPQAFKGLGEQFAWLTQ
                     NGIVLGAISAIILNFFFNGIKYKQTEENVK"
     gene            complement(18181..18759)
                     /gene="xpt"
                     /locus_tag="NBI57_000689"
     CDS             complement(18181..18759)
                     /gene="xpt"
                     /locus_tag="NBI57_000689"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HCD1821655.1"
                     /translation="MELLGQKVKEDGVVIDEKILKVDGFLNHQIDAKLMNEVGRTFYE
                     QFKDKGITKILTIEASGIAPAIMAALHFDVPCLFAKKAKPSTLTDGYYETSIHSFTKN
                     KTSTVIVSKEFLSEEDTVLIIDDFLANGDASLGLYDIAQQANAKTAGIGIVVEKSFQN
                     GHQRLEEAGLTVSSLCKVASLEGNKVTLVGEE"
     regulatory      complement(18990..19092)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            19272..19679
                     /locus_tag="NBI57_000690"
     CDS             19272..19679
                     /locus_tag="NBI57_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HCD1821656.1"
                     /translation="MADITVVNDTGELYNVINQKKSEGYLESELTIISKSKLHLNDLH
                     DSEISLISTSGTFSDRMTKLLTGEDGEHAVLSRYNLAPDELEKYKQLILDDKMLVVAV
                     RDKSSHKEVQEHNSAYEEIDITHFAEASKGPKA"
     gene            19822..20484
                     /locus_tag="NBI57_000691"
     CDS             19822..20484
                     /locus_tag="NBI57_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1821657.1"
                     /translation="MEFKTIQSVKDPHYNEALKLYDTKLNLSLTEDKRIIKRSLENNK
                     TENDYVFIVGLENDVVVSLATAHYEATTNSAFLIYLFAKDLPNHDELMTQTLHQIEHE
                     LNLLSNLVHSRSINFLMMEVPKEPADLTQDNGLIIEHRRQFLYEHQFERQTEIDYIHP
                     TNDGHTKKVDLFLKKNIKLTKDIYGTSVKSNYILKYVFANGIAREIIYALLEEMDLRK
                     PF"
     gene            20602..21558
                     /locus_tag="NBI57_000692"
     CDS             20602..21558
                     /locus_tag="NBI57_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1821658.1"
                     /translation="MLTKEFAQRVELSEKQVRKIVQHLEERGYQLSKTEYRGREATDF
                     KEEDIELFKDIADKVKQTNSYDLAFDELEKEKDFLQVIVKNDDKNLPTNQNVAQLVED
                     LRLEIQKMREERHLLGQMMNQVHQQQQELKELQNQLTSKIDSNSESLKAIQTSQEAIQ
                     AAQASQAKVLAESTNKVEKNAVTEDKADSKDSKVAGVNTSTDAKTDTKAENAGDGTAT
                     KVDKEDQISATEAIEKASVEQSKNENAAETSNKEATVDADAQHDAEQQVAEAHAEASK
                     QATSNDSLEAKAENDSTASQSEMSEPKPQEEKKGFFARLFNL"
BASE COUNT         7142 a         3827 c         3152 g         7659 t
ORIGIN      
        1 gactttcatt atttcattta gttgttttac tggattatcg tttactacaa ctgatgatgt
       61 tactctgttc tgctctttga attacaaaaa caacttaaca acagtaatgt atttaacaca
      121 gttccttata taaggacaca gatgtcaatt cacattttgt cgttaagtcg cttcttaagc
      181 tattatgcta ttaactcaaa ctaattcaaa ttcacttcaa tatttttaat ttgcttacta
      241 tccattacat caaacatacg atcaaaactt aatttttcac ttaaatcaat tacatatttc
      301 ttttcgtctt tcatattaat aacgattcta cctttatctg acgcgccttt atacaatcta
      361 tatttttcga ctaacatttt tcttatttta aaatccagtt ccttcaacga tacttcttcc
      421 ttttgaataa agaacaactc atttatcgaa aagccatgtt tagcatcaac ttttttaact
      481 tgtagacttg gattacttac aaatccaaac actgatctca catttttctt tgttataccg
      541 ccaacgctat atatgccacc ttttatatct attaattcag gtactgcaaa gacatcaagg
      601 ccatgtgttt tttctttata tttattttta tcgtcaccaa gcagaaacac tttgaaagtt
      661 ttatttcgtt ggttaaagtt taaaacgtta gaaccatagt aatcttcact tttaacgcta
      721 atgtttgttg attcaaaact ttcttcagaa tagtattgat gtagcatatt gatataatat
      781 ttgcgttgtg tctcatcaag ttctactttc gcgtgaacag ctttactttc tgctgtaaac
      841 acacctgtag ttaatattcc taatgctaat gttgcttttg ctaatgcagt caatttcata
      901 tttatttgct ccaatcttga tatattggat tgttatgatt acgtaatttg aactatctgt
      961 ctttattata aggtgcatca aaacagactg acattaacgc tcatttaaat aaacttgaat
     1021 cattattaat gaaaatcaaa cgtattttaa cattcgtatt aactgatttt taatagcaac
     1081 ataaattttt ttgggggaat cattgttctt tccgattagt tttagatctt aagttattcg
     1141 atacaaagtg tacaaaaaag cagcttaata acgtagtcaa tgttcgatat gtttttatta
     1201 ttaaaacacg tatcaaaatc attaaacatg ggattattaa accgcttctt aagcttttat
     1261 tctatatatc attgattatt tcaaattcac ttcgatgttc ttaatttgtt cactattaat
     1321 gacatctgcc atacgctcga aatctaattt atcacttaaa tcaatttcat acttattttc
     1381 atctttcata ttaataacaa ttctaccttt atcagctgac ccttcataca gtttgtattt
     1441 tttaatcaac agttttctta ttttaaaatc aagttccttc aatgacactt cttccttttg
     1501 aataaagaaa aattcatcaa tagaaaaacc gtctttatcg tctatttttt taactagtaa
     1561 gttcggcgtt cttagagact caaatattga ttttacgttt ttcttcgtta caccactaac
     1621 actaaatatt cttccatcta aatctactaa ttctggtacc gcaaagacat ctaaaccatg
     1681 tgttttttct ttgtatttat tttcatcttt tcccaataaa aatacttgga acttttgatt
     1741 ttgttggtta aaaagcaaaa cgttagagtc ataataacct tgactttggc cactaacatt
     1801 tgttaactca taactaggtc ctgaatagta ttgatgtaac ttgtttgtat catataaacg
     1861 gttcattttt tcatactttc cttttgcatt aaccgattga ttttctgtta tcattacact
     1921 tgttgttaat attcctaata caaatatcgc tttagctatc gctgtaaatt tcatattcat
     1981 atgctccaat cttaatatat tgggttgctt ttattacgta atttgaataa tacacattta
     2041 ttataaaggg cattgagaca gactgacatt aacactcgtt taattaaaat taaatcatgg
     2101 ttaatgaaga actaattaat attcaatgga catcactttc agaagtaatg tgaattttta
     2161 gtacgctcat accctgcgtt ataaaacata aaaacagcct aacgacactg ttgatttaga
     2221 tacgcccctt tttggaggat gtacgtattt attcaacatt tcatcgttaa gctgcttcaa
     2281 atttattact ttaaagtcat tgattgttta atttaaattt gattaatagt cactgctata
     2341 ttttgaatat ccttactatt caacacatca cccatgcgct cgaattgcag tttatcacct
     2401 aaatcaatta cttccttttt ctcgtctttc aaattgatag tgatcttacc gtatttagtc
     2461 gtacctttat ataaaccata tttttcaact aattgctttc taattttgaa atcaagttct
     2521 ttcagtgaaa cttcttcttt attaattaaa aatgagtcaa ttgatgcatc taaatttccg
     2581 ccatacactt tattaacaaa taaatgtgta ttagtttcag aagatttgtt atttttctta
     2641 gtcacaccac caacagtaga tagtctaccg tttggatcaa ttaattcttt taccacaaag
     2701 acattctggc cttcaaggcc ttttttatat ttatctttat cttttcctaa taagaataat
     2761 tggtgatttt gtttttcttg gttaaagcgt acaacgttag aaccgttata gttttcaacc
     2821 ttaccactaa tattactgaa ttcaaaactt tctgatgagt agtatcgata taagtcttta
     2881 atatcatata aatgttgtac tctctcttgc ttttcttttg cgtggactgc ttggccttct
     2941 gatgtaatca caccagtagt taataagcct aatgccaatg ttgctttagc taacgttttt
     3001 aatttcatag tactattctc ccaatctatt tataaatttt gtcttaatat atttttatat
     3061 gattaattca attacgtaat acgaacaatc tactatcatt atataagcga gatactgcgc
     3121 tatgaattaa cctcttttta actatttttg aaccaacgtt aagttagtta atgaaatcct
     3181 aacagtatta attatttttt ataacacaat gctaattttt taactattca cactttccct
     3241 caatttaatt atgttttcca cattgtttca tgtcactaaa gcgacatcac gcatctataa
     3301 gtatcaacta tttccacaag ttttattggt gtttttatta ttcatcgata cgcttcattt
     3361 tcatctcgcc aacacaaaaa agaagctaag caacttatgt tgcctaactc ctctatacta
     3421 tccatatttt actattatcc atatttcatt gaattatcta atgttggctt ctattttttc
     3481 aatatttcta ccgtcaatga catcactcat gcgatttgtt tgtaattttt tattaagttc
     3541 aaacgtataa tagccgccat ctttcattat cacttttatc ttactatctt taggaaactt
     3601 tttatacaga tcaaaatttt gaattaaata ctgtctcaat ttaaagtcga gttctttaag
     3661 tgaaatctct tctttataaa tgtagtgtac tctaccgtac gtagcaatac cgtcaccttc
     3721 atctctcttg atttgaaatc ttggtgcgtt tatataatca taataagcgt cttgattttt
     3781 cttagtgaca ccaccatatg aaaacactgt gccgttgcga ttttctgctt ctttaacaac
     3841 aaatatgtct aatcccggat ttttgcgtgc tttaaatctt tcaatatctt taccaaatat
     3901 ctgtactctt gtgaattttc tatttttatc aaagataagg taatgcttgc cacctttgct
     3961 ataacgataa ccagtaacat ttttaagttc cttacttgcg ccactatagt aatctcttaa
     4021 gtcaaagata tcttttgtca cattttcata ttttgcttca tgttcactcg catttacagt
     4081 ttgatgcaat gacgttattg ttcctgttgc taaaatacct aatgctaaac ttgctttcgc
     4141 aattgctgcc attttcatag ttgtatgctc cattcgtaat tattagattt gttcgcttac
     4201 gtctattgaa tcatacagct ttattataat tggcgtattt gacctttcac attaaaccat
     4261 ctttaataat cattgaatca ttattaagta aattaaggaa tctataatgt tcgttaaata
     4321 aaactgattc cgttgtgctt cacacccgat agatagggat ttacagataa attcaggact
     4381 cttccacgtc atatttggac ccatcgaaaa ttcgggttct caaatcatcg aacataacaa
     4441 aagaagctaa gcaacatgta ggccgttgtc actgaacttc ttgtttttcc gatgacagct
     4501 tctatttaga gaatgtcatg attattttat attcacttca atgttatcaa tattagtgcc
     4561 atctatgacg tctgccatgc gattttcttg taattttttg tgtaattcaa acgtgtactt
     4621 tccaccgttt tttcatgtta ataacaattt tacctgaacc aacgttaccg tacagattat
     4681 gattttcaat aagttgtttt ctcaatttaa aatcaagttc tttcaaggaa atctgctctt
     4741 tagtaatctt gaattctgaa acatcatgag agattgtacc tttattatct tctttagtaa
     4801 ttcttactcc tgctttgtga tcaacttttt tactattact ctttgtgata ccaccgaccg
     4861 agtatctttc cactccatat ttattttttt cttctaaaac gacaaataca tcgacattcc
     4921 tatgtactcc ttcatcatat tttttatcgt cttttccaac taaagctatt ttatatatga
     4981 aataatctgg aacaatattc ataaatctta ttgtcgtcca tttttttaaa ataataccaa
     5041 tctcattttt aaattctaaa cttggtttcg tataatacgc tcttaaatct ttaaatttag
     5101 gatttatttc tgttggtact tgttttgtgg ttggcgattg tggtgtttcc actttagttg
     5161 aagatggtgt tgtcgcgttc ggtgtttgtt gcggtatttc cactttagtt gaaggcggtg
     5221 ttgtcgcgtt tggttttgat tgcggtgctt ctactttagt tgagggcggt gttgattgtg
     5281 gtgcttccac tttagtggaa gatggtgttg tcgcgtttgc ttcttgcgtt gtcgttgtga
     5341 ttacacccgt tgttaaaagg cctagtgcta aacttgtttt agcaattgtt gttattttca
     5401 tagttgtatg ctccattcgt aattattaga tttgttcgat tacattcatt gaatcataca
     5461 gctttattat agatggcgta ttgctccatt cacattaaac cttgtttaac tatatttgaa
     5521 tcatcgttaa gtaaattaag aaatccataa tgttcgttaa ataaaactga ttttgatgtg
     5581 attcaacact tggcacattt gaggattcgt cacttttaag acatagaaat gccactttta
     5641 caaacaaatg aatattcgtc ttttacatca ttacgcataa taaaagaagc taagcaacat
     5701 gtaaaccgtt gtcacttaac ttcttgtttt accgatgtca gcttctattt agagaacgtc
     5761 atgattattt tatattcact tcaattttat caatgtttgt accttctatg acatctgcca
     5821 tacgatgctc ttgcagtttt ttgtgtaatt caaacgtata cttcccaccg tttttcattt
     5881 taataacgat tgttcctgaa cccatgttac cgtaaagatt atgtttttca ataagttgtt
     5941 ttctcaattt aaaatcaagc tctttcaagg aaatctcttc cttagtaatc atgtattctg
     6001 aaacatcgcg tgaaatcata ccttgattat cttttttagt aacgcttaat tctgctttgt
     6061 gatcaacttt tttactatta gtcttcgtga tgccaccgac agaatatttt tttaattgat
     6121 atttattgtc ttctaaaacg ataaatacat cgatattatc gtaaggtcca tctttatatt
     6181 ttttctcatc ttttccaact aaagctattt tatagatgaa cctatttgga ataacattca
     6241 taaacctaac cgtcgtccat ggtttgagca aaaatccaaa ctgcttttca aattcaaaac
     6301 tcggtttcgt gtaatacgct cttaaatctt catatttagg agtcatatct gtttgtgctt
     6361 gttttatggt tggagattgt ggtgtgtctg atttagtaga ttgcattggt tgtggcgtgt
     6421 ttgttgatgg aggtgttgtc actttagttt tcggcgttgt ggattcggtt gtcgtttgtg
     6481 attgttcttg tttaggcgct ggcgttgctg atatattaag cgatttctgc tcttcttgtt
     6541 taggttgtga tattttttct attttggaag ctgaggtttt ttcctcatta gtatttggtg
     6601 ccttttcgag tttaggcgtg cgttcttgtc ttgtgttagc tgcttgtgtt gtcgctgaat
     6661 ttgcacctgc tgttatgttt atcattgcta atcgctctgc tttaagcgtt ggtactttgt
     6721 caactttagt tgattgtatt ttttctgctt tgaccgattg cgtcgttact gtaattgcgc
     6781 ctgttgttaa aagccctagt gctaaactgg ttttagcaat tgttttcata ttcataattg
     6841 tatactccaa tctatattat attcgattgt ctttttacgt aatttgaatc atacaacatc
     6901 attatagatg gcgttctaag ataatcacat taaacccttt ttaacaatta ttgaagtatt
     6961 attaagtaat ttaagcaaaa aataatgagt gattatgagg ttaatatagc gtttctatgt
     7021 gcctttgaaa tgatttttaa gcattaaaaa gaagttaagc aacgtttgat cgtcacttaa
     7081 cctctctatt tcaatttcaa attatttcgt cgtcaagtat gtgtattaag cttttctaac
     7141 tttgatttca attctatcaa tatctgtgac attgataaca tcggacatac ggtcttcttg
     7201 taacttttta tccaattcaa atgtatactt tccatagtat ttctttttga cggtaatttt
     7261 ccctgtactc atttcaccgt aaagaccata attatcaata aggtattttc ttaatttaaa
     7321 atcaatctct ttcaatgaca tcgcttcttt atctatttta aatgggaaaa agtcataatc
     7381 atattcacca atatgatctg ctttaataac tcttgcttct gctattaggt cgacagcttt
     7441 atcgtttgca ctagtgatac ccccaataga gtactttgca ccttcaaatc tcttatcctc
     7501 attaacgtaa aatatattaa gattacgatg tacacccgta tgataatgtt gcttatcttt
     7561 gccaattaaa gcaaggttat taacagaatt accatctatg atattcataa atttaatact
     7621 tggttgaatg aaactgatat aacctgtcac atttttatat tcaatactag gttgagtata
     7681 ataagctttt aattttttgc tattttcact tattacaata ggtttctttt cggcatgaac
     7741 tggtttttcc gttgtagtgt ttacacctgt tgctaatatt cctaataaca aacttatttt
     7801 tgcaatattt ttcattttca tagttgtatg ctccaatcta ttataattag attgttttat
     7861 tacgtaattt gaaccataca cccatattat aggagctgta ttcggatatt cacattaacc
     7921 tgtttttaac tattcataaa atatgattaa gctatttaag caaaagataa tgcgtggtgc
     7981 gactgtttgt gtgagttttg taacttttcc cctcaaaaaa agaagctaag caacagtacg
     8041 tcgcttaact tccatatatt tccataacaa gcactattca acatagcgct ttgttgttag
     8101 aaagtattat ttcatttcta ctagaatttt ttgtatttgt ctgccgtcga tagaatcacc
     8161 catacgttct ttttcaagtt tttgacttaa atcgatagta tgtgatttgc catctttcat
     8221 tgtaattgta atttgacctt gtttaagacc atttgaatac aagccgtgtt gtttgattgc
     8281 acgttctctt aatctataat caagttcttt caatgagatg tcttctttat aaatttggta
     8341 taaactactt tgtggttctt ctttaccttt cttgatttca aggattggtg tgtgtatata
     8401 gtcaataaaa ggttgactgt ttgtttttgt tacgccacca attgagtaat ttgttgcttg
     8461 tctactgtca ccttctctaa ggataaacac atctatattc gagttgtctc catctttaaa
     8521 tttgtcttta tcagatccaa ctaaagaaat ttgagaatat tgattgtcta ctataacatc
     8581 tatataatct ttaccttttt cagtatattt atatccagta acatttttac gctctaaaac
     8641 cggtttatta taatagtgtt tcaactctga ttcactttgt tgtttaactt ctgttttcgc
     8701 ttgtactgat tgtacattcg atgtaattac acctgttgct aacattccca atgctaaact
     8761 tgcttttgct atcgctttaa atttcataat ttttagcccc aatctattta aatttgattt
     8821 ttcaattacg taatatgaac aatctaccta cattatattg aggcgctatc ccctttcaaa
     8881 ttaaacctca tttaactaaa cttgaatcat tgttaaggta attaataaaa tgctaactgt
     8941 tattaatcaa ttcttatccg tcgagactct tttttataca aatcacactt tataccattt
     9001 ttattttaaa aactgtaaaa ctttcagaca caaaacagac atcatattgt cgtagtacct
     9061 gattaaaatt ttcaaagata ttagtgatac acatcaacta aaacgcgtca ttacggtatt
     9121 ttcaacatta ataacatata cgtatttact atacacagcg ataataaata cagattacaa
     9181 ttttttataa aatcaaatgt tttgaaaaca atcgaataag tattatgtta tgtatataaa
     9241 gattaggaga tgaagtgaat gaatattatg ttaacaggtg ctacaggtca tttaggcaca
     9301 catattacaa atcaagccat tgcaaatcac atagatcatt ttcacattgg tgttagaaat
     9361 gttgagaaag ttccagatga ttggcgtgga aaagtttctg ttcgacaatt agattatttt
     9421 aatcaagaaa gcatggtaga agcatttaaa ggaatggata ccgttgtgtt tattccaagt
     9481 attatccatc catcattcaa acgtattcct gaagtggaaa atttagtata tgcggcaaag
     9541 cagagcggcg ttgctcatat cattttcata ggttattacg cagatcagca taataatcca
     9601 ttccatatga gcccttattt cggatatgca tcacgtttat tgtcaacaag tggcattgat
     9661 tatacgtatg taagaatggc aatgtacatg gatccactta aaccctattt accagaattg
     9721 atgaatatgc ataaactgat ttatccagct ggtgatggtc gtattaatta tattactaga
     9781 aatgatattg ctagaggtgt cattgctatt attaaaaatc cagatacttg gggcaaacgc
     9841 tatttattat caggctatag ttatgatatg aaagaacttg ctgctatttt atctgaggca
     9901 tcaggcacag aaattaaata tgaacccgtt tcattagaga catttgcaga aatgtacgat
     9961 gaacctaaag gctttggtgc attattggca tcaatgtacc acgcaggagc aagaggacta
    10021 ttagaccaag aatccaatga tttcaaacaa ttagtcaatg atcaaccaca gacactgcaa
    10081 tcatttttac aagaaaatat ttaataataa aggagcgtta cagtgaatat catctcaaca
    10141 attttaatca tatttgtggc attagagttt ttctatatta tgtaccttga aacgattgct
    10201 acaacttcca aaaagactag cgagacattt aatataagcg tcgataaatt gaaagacaaa
    10261 aatattaacc tacttttgaa gaaccaaggc gtatataacg gtttaatcgg agttttgcta
    10321 atatacggtt tgtttatcag cagtaatcca aaagaaatat gcacagctat tttagtgtat
    10381 atcattggcg ttgctattta tggtggcctt tcaagcaata ttagtatctt tttcaaacaa
    10441 ggcacattgc cagtattggc actcatatca atgctttggt aagtattggt gtttgggggt
    10501 ttggagatgt agtctggggt tgagagtttg gagggtttga gtgttttggg tgtggacgtt
    10561 cataaactca gagtatttca tcctaattat ttcaagcaga ggtgacagta gcgtttcctc
    10621 tgtttcctta tagaaaaatg atttaatgaa gaaaacccat atctgtttta atttttagct
    10681 gataaatact ccatatatta gaatggctac tttatctatt gcatcaatcc tttaaaacaa
    10741 aaaacccatg atttcgaaat tcccgtatgg tggggttcct actctcatgg atctattaaa
    10801 taaattatca ctataagttt attatttcaa tattattaac aatatatgta gtcgtaaaag
    10861 gaaagaggac atgagaactt cggtgttgat tggcattaca taacgcttcc aaacatatta
    10921 tttggtaaca ataagaaact atttacacaa cctattttgt atagtaaaat tattttataa
    10981 gatttaaatc caattgtaca aggagtgaat atcatggtac cagaagaaaa aggttctatt
    11041 actttgtcaa aagaagctgc tatcatattt gcaaccgcaa aattcaaacc atttaagaac
    11101 agaattaaaa ataacccact aaaaacaaat ccatttctta aattacatga aaacaaaaaa
    11161 tcttaataac tttatttata gcatttctaa tctcagaaat gctataaagg acaacgccct
    11221 ctttcataac tatataagtc aagaaaccaa gacatttcaa acatctttaa acccttcaat
    11281 agcacattcc ctaaaacata aagcaataac aatgaacgat caaaattcct tacgtactta
    11341 tgatgtgtca gtgaatcgtc tacacttaat tgaacaaatt ctatgagaat agatattgtt
    11401 aaattaagaa agtagtcaat cttattatga caaaagaaag aagaacattt agttcagagt
    11461 ttaagttaca aatggttaga ttatataaaa atggtaagct taagaatgaa attatacgaa
    11521 agtatgattt aaaaccctca attatctcaa attcgataaa acaacaccaa aatactggac
    11581 ccttcaatca tcaagataat ttaaaaagtg atgaaaaaga gttaataaaa ttacgcaaag
    11641 aagttcaaca tttaaaaatg gaacatgatg ttttaaagca aattttaaag ttgatttaga
    11701 aactgaactt tttaaagata cattacttga agattctaat ttatctgatt atataaagaa
    11761 ccaacttcca acgatatgga gagactaaga aatagtttaa atttatttaa tttcaataaa
    11821 aactcataat aatatttaag taagttagat tgcttttagc attaggaatt cgcttataat
    11881 tagatgttca atatagactt ttttacacat acatgaacta taaaaagcga ataaatagaa
    11941 ggtcattgcg aaatttgcta aatacaaaat tgatgagttc cacctaataa aaattgttgg
    12001 ttttatagta acatctattt tcgtaccatt catgttaatt tcagtgctaa taatgctgat
    12061 ataaagaaga ataaattgat taggagaaca ctatttaatg tctaaaaata tcaatcaagc
    12121 aaatagtaaa ttaaatacgt caaacaaaaa actaaaacaa gcttattcaa aatccgatag
    12181 taaaaacatg aaagttatat acatgcctca ttggctagag ttttattcaa ttgctataca
    12241 tagcgatgtt acaagcaata aaaaaagata ttataaatca tttagtagag gaacagtggt
    12301 atatgtaaaa ctagggagta atattggaag tgagttttct ggtaatcatt tttgtgtaat
    12361 attagataac aaagacaata aaggaaaaga aacagttaca atagttccac tttcttccaa
    12421 aggtaataaa aattatttga agttaaacga atcggtgcta aatctaacag caacagattt
    12481 aaaaaaacag attatcgata tcagtagtaa agtacaagca ttaacccaaa aatatagaga
    12541 tattgacatt aaacttagca cagaagataa aaagcttttg tctaacttaa atcaaaaagc
    12601 taatgaactg tatagagtga taggtgttta ttccaagcac aaagggaaag atacatacgt
    12661 taatatatca gcaattacaa caataagcaa aagacgtata agcaaaataa atgattctga
    12721 tcctactgga acgataataa tttcagaaga tgacatgaat gaaatcgaaa agcaattaaa
    12781 gataaagttc ttttctaatt gaattgtaat attacatctg atataattga tttacaaatt
    12841 cccggtaacc attccggctt cattctcttg aagaatgaca tattctcagc gttttagctg
    12901 aaggtcagat gatacgtcat ctggcctctt tttttatata aatgcccttt aagtgaaata
    12961 tacaacaatc tgcaaccaat atagacatca caaaacacac aataaattac acaatgtgtt
    13021 ataagccaca tgtcattatt atcgtagcta gctgcgtcac tcaaaacgaa tacggaacgc
    13081 gttggttatt ccaatcataa tattactctg caaatacaca gccctgtatc cgagtaaatg
    13141 aatagagatt tgaacaaatg aaaacaatca actaatggaa aggataaaat attatgcaca
    13201 acaaaaatga attcaacaaa aaactgaaag attttatagg tagcgataaa cggatggctt
    13261 tagtaaaggg ttatattaac gagtataaat taacaactgt tttaagagct ttaaataatt
    13321 ccgattacac aaaagggacg atacacactc gttcaatagg caacctaaaa caaatagtag
    13381 ataatcgaat tattcctaaa aatataactc aaaacaaaat cttcaaattg agtaatttaa
    13441 ccttagaggt taacctctat gaaagaaaca caatttatca cggagacttt gctatttact
    13501 atcctgtaga atctgctcta ataatagaaa cagatactcc taggctaatt aatcatataa
    13561 ataacaaccc agtccaaaaa atatttataa taacaacaaa taactggagc tttaatacac
    13621 ttgaattaga aaaaatagta gacgaaacta taatttatga tttaaaacaa gaagatccta
    13681 aacaatatga aattttatat aaaaataaac acggaaaatt gccttattga tgaattaaaa
    13741 cgcaaactta gaacacctta ccccttttcc gtccttattg taattttcca actactcaaa
    13801 ttaaaaacca cccgtgcaaa caggtggttt tattatatat aattattccc actcaattgt
    13861 gcttggtggt tttgatgtaa tgtcatagac tacgcgattg acgtggtcta cttcgtttac
    13921 gatacgactt gaaatctttt gtaagacttc ccaatcgatg cgtgcgaagt cacttgtcat
    13981 gccgtcgata gatgttactg cacggatacc tactgtgtga tcatacgtac ggtagtctcc
    14041 cataacacct actgattgaa tgtttggtaa cactgtgaag tattgccaaa tttctctttc
    14101 aagaccttct tctctaatca cttggcgtaa aatcgcgtct gattctctaa cgatttctag
    14161 tttatcttca gtaatttctc caagtacacg aataccaaga ccaggtcctg ggaatggttg
    14221 tctccacact aaatgttctg gaatacctaa ttcaatacct aatttacgta cttcatcttt
    14281 aaacaatgta ttgattggtt cgattaattc gaattccatg tcttctggta atccaccaac
    14341 attgtggtgt gatttgattg tttgtgctgt cttagtacct gattcgatga cgtctgtata
    14401 tagtgttcct tgcgcaagga agtctacacc tttaagtttt gatgcttcat catcaaatac
    14461 atatacaaat tcattaccaa tgattttacg tttttgttca ggatctgaaa caccttttaa
    14521 tttattcata aaacgatctt tcgcattaac acgaataata ttcatgttga acccttcacc
    14581 gaattgttcc ataaccatgt cgccttcacc tttacgaagt aagccatggt ctacaaagat
    14641 acatgttagt tgatccccaa ttgctttatg caataataca gcaacaacag atgaatctac
    14701 gccaccactc atcgcacata atacacgacg gtcgcctacg cgttgacgaa tcttttcaat
    14761 ttcaatttcg ataaagtttt ccatcgtcca ttgtccttta caatcacaaa cacgacgtac
    14821 gaagttgttt aataaatcat taccatattc tgtatgacgt acttctggat ggaattgcac
    14881 accataaatg cgacgtttct tatcttcaat tgctgcatag tctgtgcttg ggctatcagc
    14941 gataacttca aagccttctg gaatttcaat aactttatca gaatgactca tccaaacagt
    15001 ttgttctgct ggtaagccag cgaataactc atctgacttc gcattaatga ttgctttacc
    15061 gtattcacgt tcattggcac gttcaacttt accacctaat aatttagtag ttaattgcat
    15121 gccgtaacaa ataccaagta ctggaattcc taaattatat atttccggat caattgtaaa
    15181 tgaaccttct tcataaactg aatttggacc acctgataag atgatacctt ttggattcat
    15241 tttcttaatt tcttcaattg aaatttcatg atcgtgtaat tcactataaa cgcccatttc
    15301 acgaattcgg cgtgtaatta attggttgta ttggctacca aagtctaaga caaggattaa
    15361 ctcttgttct tttgccattt ccatatttgt cgttctcctt tatcttaatt agaatgagta
    15421 gttcggtgat tctttcgtaa tttgaatatt atgtggatgg ctttctgcta aaccagcagg
    15481 acccatacgt gtaaattgtg cttcttcgcg taattctctt aaatcgtgtg aaccagtata
    15541 acccatacca gcacgcacac cgcccattaa ttggtaaatt gtatcttgta acgcaccttt
    15601 ataagccgta cgtccttcga taccttcagg aacaaatttc ttaggcgctt tgtcctcttg
    15661 gaagtaacgg tcgtttgaac ctttttccat cgcacctaaa gagcccatac cacggtatac
    15721 tttatattgt ctaccttgga aaatttctgt agcacctgga ctttcttcag tacctgctaa
    15781 taagctacct aacataaccg catgtccacc agcagctaat gctttaatga tatctcctga
    15841 gaatttaata ccaccatcag caatgatagc tttaccatgt ttgcgtgctt cagttgcaca
    15901 atcataaatt gctgtaattt gtggtacacc aacacctgct acaacacgcg tcgtacaaat
    15961 tgaacctggg ccaataccaa ctttaacaat atctgcaccc gcttcaaata aatcttttgt
    16021 tgcttctgca gttgctacgt taccagctac taatgtgatt tctgggtaag tcttcttaat
    16081 atgtttcact tgatcgataa cgcctttaga gtgaccatgt gctgtatcga taactaagac
    16141 atccacacct gcttcgacta atttttgagc acgaatatca gtatcttttg aaataccaat
    16201 tgctgcggct acaagtagac gaccatgttc atcttttgct gcattaggga attcgataac
    16261 tttttcaata tctttaatag taataagacc ttctagacgt ccgtctttaa ctaatggtaa
    16321 cttttcaatc ttatgttttt ggagaatttt ttctgcttct tcaagtgttg tattcactgg
    16381 agctgtaatt aaattttctt gcgtcattac atctacaatt ttaattgaga agtcttcaat
    16441 aaaacgtaag tcgcggtttg ttaaaatacc tactaagttg cgatcttctt tattatcaac
    16501 aattggtaca cctgaaatac ggtatttacc cattaatgct tccgcttcat aaacactttc
    16561 ttctggtgtt aagaaaaatg ggtttgaaat gacaccattt tctgagcgtt ttactttttg
    16621 aacttcgtcc gcttgttctt caacgcccat atttttatga ataacaccta aaccaccttg
    16681 acgagccata gcaatcgcca ttttagattc agttactgta tccataccag cagaaataac
    16741 tggaatattt aatttaactt tgtctgataa ttgtacgctt aaatcaacgt ctttcggtaa
    16801 aatatcagat tgtgctggaa ttaataacac atcatcaaac gttaatgatt cttttgcaaa
    16861 tttactttcc cacattaaaa acagcctcca tttttcaaat taattagtta tattatttca
    16921 cattttcttc cgtttgttta tactttattc cattaaaaaa gaaattaaga ataattgcag
    16981 agattgcgcc taaaacaatt ccgttttgag tcaaccatgc aaattgttca cctaaacctt
    17041 taaatgcttg tggtacagcg cttataccag tacctaatcc tactgataca gcaataatta
    17101 ataaattgtt ttgattttta aaatcgatat gtcctaatat actaacacca tatgccatta
    17161 ccatgccaaa catagctatc atcgcaccac ctaacacagg tagcggtatg atatttgcta
    17221 atgcaccaag cttaggtata caaccacata taagtaataa cacgaccatg ccgtatataa
    17281 cattattttt cttagcgccg gataaagaaa caagtcctac attttgcgaa taggctgtat
    17341 acggaaatga attgaatata gaacctaaca ctatcgctag accttccgca gtataacctt
    17401 tacgaaaatc ttttctttct aacttcttac cggtaatttc acttaacgca tgatagacac
    17461 ctgtcgactc aattaaacta acgatagcta caataaagaa cactaacgtc gatgtcacat
    17521 caaagctaaa tcctgagaat ctaaacggca ctgggatgcc taaccaaccg gcatgattga
    17581 cttgattaat atcgaccatc ccaagtaagc cagcacctat cgttcctaaa acgagtccaa
    17641 ttaatatggc aatactctta ataaatccag ttgtgaatct ttgtaaaaca agaataatga
    17701 ttaatgtcat taaacctaac aaaatgttct taacatctcc atagtccttt gcaccttgac
    17761 ctccagctaa gtaattcatt gctactggca ttaaattgat accaatgata gtaacaacac
    17821 tacccgttac tactggtggg aagaatttta caagatgtga aaagaaaggc gcgatgataa
    17881 taactaatat ccctgataaa aatagcgaac cgtaaagtac atctattcct ttcgtttgac
    17941 caattaaaat catgggcgca acagccgtga atgtacatcc aagaacgatt ggtaatcctg
    18001 ttcctgttac tttattggct tgtaaaaatg tggcaacccc acacataaat atatctactg
    18061 taactaagta agcgatttgt tcaggtgtaa acttcaaact tgtaccaaca atgattggaa
    18121 ctaagatggc acctgcgtac atagctaaaa gatgttgaac acttaggatt aaatttttca
    18181 ttattcttct cccaccaatg tcactttgtt tccttctagt gaagcaacct tgcagagaga
    18241 agaaactgtt aaacctgctt cttctaaacg ttgatgccca ttttggaaac tcttttcaac
    18301 aacaatacca ataccagctg tcttagcatt cgcttgctgt gcgatatcgt ataatcctaa
    18361 tgaagcatca ccatttgcta aaaagtcatc gatgatgagt acagtatctt cttctgataa
    18421 aaattctttt gaaacaatga ccgtacttgt tttattttta gtaaatgaat gaatagatgt
    18481 ttcataataa ccatccgtca aagtgctagg ttttgctttt ttcgcaaata aacatggcac
    18541 atcaaaatgc agtgcagcca tgattgcagg tgcgataccg gaagcttcaa tggttaagat
    18601 tttagtaatc cctttatctt taaattgctc gtaaaaagtg cgaccaactt cattcattaa
    18661 ctttgcatca atttgatgat ttaaaaatcc atcgactttt aaaatcttct catcaatgac
    18721 aacgccgtct tcctttactt tttgtcctag taactccact cttaaaacct cctcagattt
    18781 gtgtgaaaca ttttgctctt aaattggtgc tagatacaaa aaaatcccca aactaaataa
    18841 tagtttcagg gtttatgagt gaacgaacat gcataacgaa tttgtcatgc aatcaatgta
    18901 aaagaagttt catcaaataa actgtgacca tcatataaaa tgatataaat cacccaccat
    18961 ggttacaatt taatggctga agctactcct agtattgtgt tgttactcat agtcatgtcg
    19021 ttcaaggcaa catggtagaa acttctaaag ccatattctt tagattatat gagtttatgt
    19081 aaattattta acgataatag caaattttcg gcattttttc aataactgct taggtaatct
    19141 tttaatagtt ttataaatgt acagagacgt ttaatgcgaa tgaagactta taacttaata
    19201 atgtaagggt tttactttat aataagaagg gtaaaagatt aaataaacaa atttttaaag
    19261 gggcgacaac tatggcagat attactgtag taaatgacac tggcgaatta tacaacgtaa
    19321 tcaatcaaaa gaaatctgaa ggttatttag aatcagagct aacaattatt agtaaaagta
    19381 aattacacct aaatgactta cacgactctg aaatctccct tatctcaaca agtggaacat
    19441 ttagtgatag aatgactaaa ctgttaacag gagaagatgg tgaacatgca gttttatcac
    19501 gctacaactt agcgcctgat gaattagaaa aatataaaca acttatttta gacgataaaa
    19561 tgttagttgt agcggttcgt gacaaatcat cacataaaga agttcaagaa cataactcag
    19621 catatgaaga aattgatatt actcattttg ctgaagcatc taaaggtccc aaagcttagt
    19681 tatcacactg aattcaaaat gagattactg tactgtctaa tttaagatag tacagttttt
    19741 tataccattt aaaagtttat gaacaatctt ttacagcagg cgattatgct ataatattat
    19801 ttaaattggg gaggtgttct tatggaattt aaaaccattc aatctgtcaa agatccacat
    19861 tacaacgaag cacttaaact ttacgatact aagttaaatt tatctttaac tgaagataaa
    19921 cgtatcatca aacgctcatt agaaaacaac aaaaccgaaa acgattatgt ctttatcgta
    19981 ggcttagaaa acgatgttgt tgtcagtcta gcaactgcac attatgaagc cacaaccaat
    20041 tcagcatttt taatttattt attcgcaaaa gacctaccca accatgatga gttaatgaca
    20101 caaacattac atcaaattga acatgaacta aacttattat caaaccttgt tcattctaga
    20161 agtattaact ttttaatgat ggaagtccct aaagaacctg cagatttaac acaagataac
    20221 ggacttatta ttgaacaccg tagacagttt ttatacgaac atcaatttga aagacaaact
    20281 gaaattgatt acattcatcc aactaatgat ggtcacacta aaaaagtgga cttattttta
    20341 aagaaaaata ttaaactgac aaaggatatc tacggtacaa gtgttaaatc aaattatatt
    20401 ttgaagtatg tatttgccaa tggtatcgca cgtgaaatta tttatgcttt attagaagaa
    20461 atggatttac gtaaaccatt ttaaacaaac ttgaaatcac taacaataac gtttaaacta
    20521 tgtatgaagc aagactgacg tttcactgcc cgtagacaaa cgtcagtata cataaagtat
    20581 atgcagaaag gactttaaag tatgttaact aaagaatttg cacaacgcgt agaactaagt
    20641 gagaagcaag tccgtaaaat tgttcaacat ttagaagaac gtggttacca actaagcaag
    20701 actgaatatc gtggtcgtga agcaactgat ttcaaagaag aagatatcga gcttttcaaa
    20761 gacattgccg acaaagtaaa acaaacaaat agttatgatc tagcgtttga tgaattagaa
    20821 aaagaaaaag acttcctgca agtcattgtc aaaaacgatg acaaaaactt acctactaat
    20881 caaaatgtcg ctcaactagt agaagattta cgcctagaaa tccagaaaat gcgcgaagaa
    20941 cgtcacctac ttggtcaaat gatgaatcaa gtacatcagc aacaacaaga attaaaagaa
    21001 cttcaaaatc aacttacatc taaaatcgat tcaaatagcg aatccttaaa agccatccaa
    21061 acatcacaag aggctatcca agcagcacaa gcatctcaag ctaaagtatt ggctgaatca
    21121 acaaataaag ttgaaaagaa tgctgttact gaagataaag ctgacagtaa agattcgaag
    21181 gttgctggag tgaatacatc aacagacgct aagacggata ctaaagcaga aaatgcaggc
    21241 gatggcactg ctactaaagt agataaagag gatcaaattt ctgcaactga agcaattgaa
    21301 aaagccagcg ttgagcagtc taaaaatgaa aatgcagctg agacttcaaa taaagaagct
    21361 acagtagatg cagacgcgca acatgatgct gaacaacaag tagcagaagc ccatgcagaa
    21421 gcttctaaac aagctacttc gaacgattca ttggaagcga aagcagaaaa tgacagcaca
    21481 gcttcacagt cagaaatgtc agaaccaaaa cctcaagaag agaaaaaagg tttcttcgca
    21541 cgtttattca acctgtaaac ttgtgatttg aacacaaatt taatactcaa ttcatatata
    21601 gctttgattt aaaaactgat tttttataaa cctatagaac tgtaatttac tttcaaattt
    21661 aatactcaat tcataaaacc tgaaacaagc tgatatactc cattcaatat ctactttgaa
    21721 gagagcatat caaactaatt tgtttcaggt tttttaatta ttcattatga ttaatattga
//
LOCUS       DAHHSC010000023        21547 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.023, whole genome shotgun sequence.
ACCESSION   DAHHSC010000023 DAHHSC010000000
VERSION     DAHHSC010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 21547)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21547)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21547
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.023"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            81..1265
                     /gene="rocD"
                     /locus_tag="NBI57_000693"
     CDS             81..1265
                     /gene="rocD"
                     /locus_tag="NBI57_000693"
                     /EC_number="2.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine--oxo-acid transaminase"
                     /protein_id="HCD1821659.1"
                     /translation="MNSIIELTDYYSSNNYAPLKLVISKGKGVKVWDTDGKQYIDCIS
                     GFSVANQGHCHPTIVKAMTEQASKLSIISRVLYSDNLGKWEEKICHLAKKDKVLSLNS
                     GTEAVEAAIKIARKWGSEVKGITDGQVEIIAMNNNFHGRTLGSLSLSNHDAYKAGFHP
                     LLQGTTTVDFGDIEQLTQAISPNTAAIILEPIQGEGGVNIPPKGYIQAVRQLCDKHQI
                     LMIADEIQVGLGRTGKWFAMEWEQVVPDIYILGKALGGGLYPVSAVLANNDVMRVLTP
                     GTHGSTFGGNPLAIAISTAALDVLKDEQLVERSERLGSFLLKALLQLKHPSIKEIRGR
                     GLFIGIELNTDAAPFVDQLIQRGILCKDTHRTIIRLSPPLVIDKEEIHQIVAAFQDVF
                     KN"
     gene            1304..2335
                     /locus_tag="NBI57_000694"
     CDS             1304..2335
                     /locus_tag="NBI57_000694"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HCD1821660.1"
                     /translation="MIKVGIVGGSGYGAIELIRLLQTHPHVTIAHIYSHSKVDEPLKL
                     TFPHLQHIMQHFEALTVDNNDCDVIFFATPAPVSKTCIPPLVEKGIHVIDLSGAFRIK
                     NREIYEAYYKETAAAQDDLNHAIYSISEWQSFDNNGTKLISNPGCFPTATLLALHPLI
                     SEKIVDLSSIIIDAKTGVSGAGRSLSQRVHFSEMNENLSAYAIGNHKHKPEIEQYLSI
                     IAGQDVSVIFTPHLVPMTRGILSTIYVKFSSEYTTESLHKLMTSYYANQPFVRIRDIG
                     TFPTTKEVLGSNYCDIGIYVDETTQTAILVSVIDNLVKGASGQAIQNLNILYDFEVTT
                     GLNQSPVYP"
     gene            2347..3588
                     /gene="argJ"
                     /locus_tag="NBI57_000695"
     CDS             2347..3588
                     /gene="argJ"
                     /locus_tag="NBI57_000695"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HCD1821661.1"
                     /translation="MKHQETTSQQYNFSIIKHGDISTPQGFTAGGMHIGLRANKKDFG
                     WIYSSSLASSAAVYTLNQFKAAPLIVTEDTLQKSKGKLQALVVNSANANSCTGQQGID
                     DARQTQTWVAQQLQIPSEHVAVASTGVIGEYLPMDKIKTGTEHIKDTNFATPGAFNEA
                     ILTTDTCTKHIAVSLKIDGKTVTIGGSAKGSGMIHPNMATMLAFITTDASIESNTLHQ
                     LLKSSTDHTFNMITVDGDTSTNDMVLVMANHQVEHQILSQDHPQWETFVDAFNFVCTF
                     LAKAIARDGEGATKLISVNVSGAKSISDARKIGKTIVSSNLVKSAIFGEDANFGRIIT
                     AIGYSGCEIDPKCTYVQLNQIPVVDKGMAVLFDEQAMSNTLTHEHVTIDVQLGLGNAA
                     ATAYGCDLSYDYVRINASYRT"
     gene            3604..4368
                     /gene="argB"
                     /locus_tag="NBI57_000696"
     CDS             3604..4368
                     /gene="argB"
                     /locus_tag="NBI57_000696"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HCD1821662.1"
                     /translation="MKFIVIKIGGSTLSDMHPSIINNIKHLRSNNIYPIIVHGGGPFI
                     NEALSNQQIEPHFVNGLRVTDKATMTITKHTLIADVNTALVAQFNQHQCSAIGLCGLD
                     AQLFEITSFDQQYGYVGVPTALNKDALQYLCTKFVPIINSIGFNNHDGEFYNINADTL
                     AYFIASSLKAPIYVLSNIAGVLINDVVIPQLPLVDIHQYIEHGDIYGGMIPKVLDAKN
                     AIENGCPKVIIASGNKPNIIESIYNNDFVGTTILNS"
     gene            4638..5117
                     /locus_tag="NBI57_000697"
     CDS             4638..5117
                     /locus_tag="NBI57_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YagU family protein"
                     /protein_id="HCD1821663.1"
                     /translation="MGIFIYAGIIGGLLSGIVKLGWEVMFPPRTPERNATNPPQELLQ
                     QLGFSSEFTHQTYTFSNMELPWVSFIVHFSFSIVIAIIYCILVKKYAYLAIGQGAVFG
                     IAIWVLFHLIIMPIMHTVPAVWDQPYQEHLSEFFGHIVWMMTIELVRQHFVYRYKLN"
     gene            complement(5445..6089)
                     /locus_tag="NBI57_000698"
     CDS             complement(5445..6089)
                     /locus_tag="NBI57_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HCD1821664.1"
                     /translation="MTVFVMQLQSNLKSIEELISQSRWSYKKPRTVNYRYNQDKLMHR
                     LGDILVQYGIQHDTGLLPHEWHYHISPRGKADIVQHNRDGQPIYVSLSYSYPYIVCVV
                     DKEPVGIDIEKISQRLDWRTLVTCFSTNEAHQICSLNDFYQIWTQKESFTKLIGEGLI
                     KGLDIYDMTQSHFYQSREVKFKQFIFDQFMVQVCFLGEAPWGYKKVSVFQLLSS"
     gene            complement(6102..13277)
                     /locus_tag="NBI57_000699"
     CDS             complement(6102..13277)
                     /locus_tag="NBI57_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HCD1821665.1"
                     /translation="MIMGNLRFQQEYFRIYKNNTESTTHRNAYWVKLAKNVEATKMMY
                     ALSTIVQQHASIRHFFDVTTDDNLTMILHEFLPFIEIKQVPSSSANYDLEAFFKQELS
                     TYHFNDSPLFKVKLFQFADAAYILLDFHVSIFDDSQIDIFLDDLCNAYRGNTVINNTR
                     QHAHINRNDDKDNQDASHIALDSNYFRLENNSDIHIDSYFPIKHPFEQALYQTYLIDD
                     MTSIDMASLAVSVYLANHIMSQQHDVTLGIHVPSHLPNDLHGNIVPLTLTIDAKDVCQ
                     RFTTDFNKCVLQNMSQLQCAKSSLSLETIFHCYHHMMSCCNDVIEDVHQIHDAHTSLA
                     DIEIFPHQHGFKIIYNSAAYDLLSIETLSDLVRNIYLQITEENGNKRTTVDELNLMTE
                     RDIQLYDDINLSLPEIDDAQTVVTLFEQQVEATPNHVAVQFDGVFITYQTLNARANDL
                     AHRLRNQYGVEPNDRVAVIAEKSIEMIIAMIGVLKAGGAYVPIDPNYPSDRQEYILKD
                     ATPKVVITYQALYENGKQNINHIDLNKIAWKNIDNLSKCNTLEDHAYVIYTSGTTGNP
                     KGTLIPHRGIVRLVHRNHYVPLNEETTILLSGTIAFDAATFEIYGALLNGGKLIVAKK
                     EQLLNPIAVEQLINENDVNTMWLTSSLFNQIASERIEVLVPLKYLLIGGEVLNAKWVD
                     LLNQKPKHPQIINGYGPTENTTFTTTYNIPNKVPNRIPIGKPILGTHVYIMQGERRCG
                     VGIPGELCTSGFGLAAGYLNQPELTADKFIKDSNINQLMYRSGDIVRLLPDGNIDYLY
                     RKDKQVKIRGFRIELSEVEHALERIQGINKAVVIVQNHDQDQYIVAYYEAMHTLSHNK
                     IKSQLRMTLPEYMIPVNFMHIEQIPITINGKLDKKALPIMDYVDTDAYVAPSTDTEHL
                     LCQIFADILHVNQVGIHDNFFELGGHSLKATLVVNRIEASTGKRLQIGDLLQKPTVFE
                     LAQAIAKVQEQNYEVIPEAIVKDDYVLSSAQKRMYLLWKSNHKDTVYNVPFLWRLSSG
                     LNVAQLQQAVQHLIARHEILRTQYIVVDDEVRQRIVADVVADFEEVNTHFTDEQEIMR
                     QFVAPFNLEKPSQIRVRYIRSPLHAYLFIDTHHIINDGMSNIQLMNDLNALYQHKLLL
                     PLKLQYKDYSEWMSHRDMTKHRQYWLSQFKDEVPILSLPTDYVRPNIKTTNGAMMSFT
                     MNQQMRQLLQKYVEKHQITDFMFFMSVVMTLLSRYARKDDVVVGSVMSARMHKGTEQM
                     LGMFANTLVYRGQPSPDKMWTQFLQEVKEMSLEAYEHQEYPFECLVNDLDQSHDASRN
                     PLFDVMLVLQNNETNHAHFGHSKLTHIQPKSVTAKFDLSFIIEEDRDDYTINIEYNTD
                     LYHSETVRHMGNQCMIMIDYILKHQDTLQICDIPNGTEELLNWVNTHVNDRMLNVPGN
                     KSIISYFNEVVSRQGNHVALVMNDLTMTYETLRNYVDAIAHMLLSNGVGNGQRVALFT
                     ERSFEMIAAMLATVKVGASYIPIDIDFPNKRQGAILEDAKVTAVMSYGVEIETTLPVI
                     QLENAKGFVESKENEQYDDLHGNQLENTAMLDNEMYAIYTSGTTGMPKGVAIRQRNLL
                     NLVHAWSTELQLGDNEVFLQHANIVFDASVMEIYCCLLNGHTLVIPDREERVNPEQLQ
                     QLINKHRVTVASIPLQMCSIMEDFYIEKLITGGATSTASFVKYIEKHCGTYFNAYGPS
                     ESTVITSYWSHHCGDLIPETIPIGKPLSNIQVYIMSDGLLCGIGMPGELCIAGDSLAI
                     GYINRPELMADKWQNNPFGKGKLYHSGDLARYTSDGQIEFLGRIDKQVKVNGYRIELD
                     EIENVILAIRGISDCVVTVSHFDTHDILNAYYVGEQQVEQDLKQYLNDQLPKYMIPKT
                     ITHIDCMPLTTNDKVDTTRLPNPSPIQQSNKVYSEPSNEIEQTFVDVFGEVLKQNDVG
                     VDDDFFELGGNSLEAMLVVSHLKRFGHHISMQTLYQYKTVRQIVNYMYQNQQSLVALP
                     DNLSELQKIVMSRYNLGILEDSLSHRPLGNTLLTGATGFLGAYLIEALQGYSHRIYCF
                     IRADNEEIAWYKLMTNLNDYFSEETVEMMLSNIEVIVGDFECMDDVVLPENMDTIIHA
                     GARTDHFGDDDEFEKVNVQGTVDVIRLAQQHHARLIYVSTISVGTYFDIDTEDVTFSE
                     ADVYKGQLLTSPYTRSKFYSELKVLEAVNNGLDGRIVRVGNLTSPYNGRWHMRNIKTN
                     RFSMVMNDLLQLDCIGVSMAEMPVDFSFVDTTARQIVALAQVNTPQIIYHVLSPNKMP
                     VKSLLECVKRKEIELVSDESFNEILQKQDMYETIGLTSVDREQQLAMIDTTLTLKIMN
                     HISEKWPTITNNWLYHWAQYIKTIFNK"
     gene            complement(13724..14974)
                     /locus_tag="NBI57_000700"
     CDS             complement(13724..14974)
                     /locus_tag="NBI57_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCD1821666.1"
                     /translation="MKRLSTTLKVRLISNFLQLIITTAFIPFIALYLTDMLSQSIVGI
                     YLVGLVVLKFPLSIISGYLIEIFPKKLLVLIYQATMVIMLVFMGVFGSHQLWQIIGFC
                     VAYAIFTIVWGLQFPVMDTLIMDAITEDVEHYIYKISYWMTNLSVAIGALLGGLMYGY
                     SMLLLFLIAACIFLIVLFILYIWLPQDRNQVKQSDDKRHASRYQKLQIMNIFRSYKLV
                     LKDRNYMLLISGFSIIMMGEFSISSYIAIRLKDQFETISIGSYDITGAKMLAILLMIN
                     TVVVILLTYSISKVVLKIDFKKALITGLLIYIVGYSGLTYLNQFGLLVVFMIIATVGE
                     IIYSPIVSEQRFKIIPKAKRGTYSAVNALGIHFSETLARLGIVLGVFLTSLQMGLYMF
                     IVLTIGASMLVAGVFGGQKQVNTN"
     gene            complement(15360..16385)
                     /locus_tag="NBI57_000701"
     CDS             complement(15360..16385)
                     /locus_tag="NBI57_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent formate dehydrogenase"
                     /protein_id="HCD1821667.1"
                     /translation="MKIVALFPEAVEGQENQLLNTKKAIGLKTFLEERGHEFIILADN
                     GEDLDKHLPDMDVIISAPFYPAYMTRERIEKAPNLKLAITAGVGSDHVDLAAASEHNI
                     GVVEVTGSNTVSVAEHAVMDLLILLRNYEEGHRQSVEGEWNLSQVGNHAHELQHKTIG
                     IFGFGRIGQLVAERLAPFNVTLQHYDPINQQDHKLSKFVSFDELVSSSDAITIHAPLT
                     PETDNLFDKDVLSRMKKHSYLVNTARGKIVNRDALVEALASEHLQGYAGDVWYPQPAP
                     ADHPWRTMPRNAMTVHYSGMTLEAQKRIEDGVKDILERFFNHEPFQDKDIIVASGRIA
                     SKSYTAK"
     gene            complement(16559..16918)
                     /locus_tag="NBI57_000702"
     CDS             complement(16559..16918)
                     /locus_tag="NBI57_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2294 domain-containing protein"
                     /protein_id="HCD1821668.1"
                     /translation="MNTIDTHTKEQQFSNLVRSYRKEYVGKGPNSIRVSFKDNWAIAH
                     MTGVLSKVESFYLNDKRNESMLHYTRTEKIKQMYKEIDVNEMESLVGAKFVKLFTDID
                     LNDDEVISIFVFDKSIE"
     gene            complement(17135..18166)
                     /locus_tag="NBI57_000703"
     CDS             complement(17135..18166)
                     /locus_tag="NBI57_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA/acyl-ACP dehydrogenase"
                     /protein_id="HCD1821669.1"
                     /translation="MTLETLIKEQLDPHLVEVDEGTYYPRTFIQQLFVDGYFGEAALR
                     KNAEVIEAVSQSCLTTGFCLWCQLAFSTYLENATQPHLNNDLQQQLLSGEILGATGLS
                     NPMKSFNDLEKLNLEHTYVDGQLVVSGRMPAVSNIQEDHYFGAISKHESSDEFVMFIL
                     RANQDGITLVEKTNFLGVNGSATYQITLNQVVVPQSQIITHDAKQFAATIRPQFIAYQ
                     IPIGLGSIKSSLELIDAFSNAQNGINQYLEYDVEAFKKRYRQLREEYYAILDDGNLTS
                     HLSELISLKKDIGYLLLDVNQASVVNGGSRAYTPYSPQVRKLKEGFFFAALTPTLRHL
                     GKLEAELKG"
     gene            complement(18179..18940)
                     /locus_tag="NBI57_000704"
     CDS             complement(18179..18940)
                     /locus_tag="NBI57_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCD1821670.1"
                     /translation="MTRPTNNKFILPIITFIIFLGIWEMVIIIGHYQPVLLPGPALVG
                     KSIWTFIVTGEIFQHLAISLWRFVAGFVVALLVAIPLGFLLGRNRWLYNAIEPLFQLI
                     RPISPIAWAPFVVLWFGIGSLPAIAIIFIAAFFPIVFNTIKGVRDIEPQYLKIAANLN
                     LTGWSLYRNILFPGAFKQIMAGIHMAVGTSWIFLVSGEMIGAQSGLGFLIVDARNMLN
                     LEDVLAAIFFIGLFGFIIDRFISYIEQFILRRFGE"
     gene            complement(18937..19908)
                     /locus_tag="NBI57_000705"
     CDS             complement(18937..19908)
                     /locus_tag="NBI57_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCD1821671.1"
                     /translation="MKRLSIIVIIGIFIITGCDWQRTSKEPSKNAQNQQVIKIGYLPI
                     THSANLMMTKKLLSQYNHPKYKLELVKFNNWPDLMDALNSGRIDGASTLIELAMKSKQ
                     KGSNIKAVALGHHEGNVIMGQKGMHLNEFNNGDDYHFGIPHRYSTHYLLLEELRKQLK
                     IKPGHFSYHEMSPAEMPAALSEHRITGYSVAEPFGALGEKLGKGKTLKHGDDVIPDAY
                     CCVLVLRGELLDQHKDVAQAFVQDYKKSGFKMNDRKQSVDIMTHHFKQSRDVLTQSAA
                     WTSYGDLTIKPSGYQEITTLVKQHHLFNPPAYDDFVEPSLYKEASRS"
     gene            complement(19922..20662)
                     /locus_tag="NBI57_000706"
     CDS             complement(19922..20662)
                     /locus_tag="NBI57_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCD1821672.1"
                     /translation="MIKIQQLQHHFGSHKVIHNFNLDISKGEIVTFIGKSGCGKSTLL
                     NVIGGFIHPSSGRVIIDNEIKQQPSPDCLMLFQHHNLLPWKTINDNIRIGFQQKISDE
                     EINAQLKLVDLEDRGKHFPEQLSGGMKQRVALCRAHVHKPNVILMDEPLGALDAFTRY
                     KLQDQLVQLKHKTQSTIILVTHDIDEAIYLSDRIVLLGEGCNIISQYEITASHPRSRN
                     DSHLLKIRNEIMETFALNHHQVEPEYYL"
     gene            complement(21004..21516)
                     /locus_tag="NBI57_000707"
     CDS             complement(21004..21516)
                     /locus_tag="NBI57_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4242 domain-containing protein"
                     /protein_id="HCD1821673.1"
                     /translation="MTLFLLEANNLDFASTKEELEAKAASLSTKTIPTLIEVQATENL
                     THGYFIVEANDEAEAKQFLTEADISIQLVKEVRLVGKDLDEVKNGDAHVDYLVTWNIP
                     EGITMDQYLARKKKNSVHYEEVPEVEFKRTYVCEDMSKCICLYNAPDEEAVRRARKAV
                     DTPIDGIEKL"
BASE COUNT         6956 a         4146 c         3321 g         7124 t
ORIGIN      
        1 acatttgaca cttatattga acaactgtaa tatattaata ttaattcttt aaattgtata
       61 aatataaagg agggagaccg atgaattcaa tcattgaatt aactgattat tatagctcta
      121 ataattatgc accacttaag cttgtcattt ctaaaggtaa aggtgtcaaa gtttgggata
      181 ctgatggcaa acaatatata gattgcattt cgggtttttc agttgcaaac caaggccatt
      241 gtcatccaac aattgttaaa gcgatgacag aacaagcttc aaagttgtct atcatttcac
      301 gtgtccttta tagtgacaat ctcgggaaat gggaagaaaa aatttgtcat cttgctaaga
      361 aagacaaagt actctccctt aactctggta ctgaagctgt tgaagcagcc attaaaattg
      421 ctagaaaatg gggctctgaa gttaaaggca ttactgacgg acaagttgaa atcatcgcta
      481 tgaataacaa ttttcacggt cgtacacttg gctcattatc actatctaac cacgacgcat
      541 ataaagcagg atttcacccc ctacttcaag gcactacaac tgtagatttt ggagacattg
      601 aacaattaac acaagctatt tcaccgaata cagcagcaat tattttggaa ccaattcaag
      661 gtgaaggtgg cgttaatata ccaccgaaag gatatattca agctgtgcgt caactatgtg
      721 ataaacatca aatattaatg attgcagatg aaattcaagt tggtcttggt agaactggga
      781 aatggtttgc gatggaatgg gaacaagttg ttccagacat ttatatttta ggtaaggcat
      841 tgggtggcgg cttataccct gtatctgctg tacttgcaaa taatgatgtc atgcgtgttc
      901 taacaccagg tacacatggt tcaacatttg gcggtaaccc tttagccatt gcaatatcga
      961 cggcagcgct tgatgtactt aaagatgaac aactggttga acgatcagaa cgcttaggtt
     1021 catttttatt aaaagcgttg ctacaactta aacatcctag tattaaagaa attagaggtc
     1081 gtggtttatt tataggcata gagcttaaca cagatgctgc accttttgtg gatcaactga
     1141 ttcaacgtgg aatcttatgc aaagacacgc atcgtactat cattcgattg tctccacctc
     1201 tagttattga taaagaggaa atccatcaaa ttgttgcagc ttttcaagac gtttttaaaa
     1261 attaacaatt aatcatttat atatgacata ggagggattc atgatgatta aagtaggtat
     1321 cgttggcggt agcggttatg gcgcaattga attaattcga ttgttacaaa cacatcctca
     1381 tgtaacgatt gcacacatct actcacattc aaaagtagat gaaccgttga aattaacatt
     1441 tccacattta caacatatta tgcaacattt cgaagcactt acagtggaca ataatgactg
     1501 tgatgtaatt ttctttgcga caccagcacc cgtaagtaaa acatgtatcc ctcccttagt
     1561 agaaaaaggt attcatgtta tcgatttatc tggcgcattt agaattaaga atcgtgaaat
     1621 atatgaagca tattacaaag aaactgctgc agcacaagat gatttgaatc atgctattta
     1681 cagcatttca gaatggcaat cgtttgataa caatggaacg aagctcattt ctaatcctgg
     1741 ctgtttccct acagcaacat tattagcatt acatccactt attagcgaaa aaatagtaga
     1801 tttgtcatct attattattg atgctaagac cggcgtgtca ggtgctggtc gttcattatc
     1861 acaacgtgtt catttttcag aaatgaatga aaatctaagt gcttatgcaa tcggaaacca
     1921 taaacacaaa ccggaaatcg agcaatattt atctatcatt gcgggtcaag atgtatcagt
     1981 catatttaca ccacatctcg taccaatgac acgaggtatt ttatcaacaa tttatgtcaa
     2041 attttcatct gaatatacga ctgaatcatt acataaatta atgacctctt attatgctaa
     2101 tcagccattt gtcagaatta gagatattgg gacttttcca accacaaaag aagtactcgg
     2161 tagtaactac tgcgatatcg gtatctatgt agatgaaaca acacaaacag caattttagt
     2221 atcagtgatt gataaccttg tcaaaggcgc aagtggtcaa gccattcaaa atttaaatat
     2281 attatatgat tttgaagtaa cgactggcct aaatcaatca ccagtttatc cataaggggt
     2341 gttagaatga aacatcaaga aacgacatca caacaatata acttttcaat tattaaacat
     2401 ggcgatatca gtacacctca aggcttcacg gctggtggta tgcacatcgg tttacgcgct
     2461 aacaaaaaag actttgggtg gatttactca tcgtctttgg caagttcagc tgccgtatat
     2521 actttaaatc agtttaaagc tgcaccactt attgtcactg aagacacttt acaaaagtct
     2581 aaaggaaaat tacaagcact tgttgttaat tcagctaatg caaattcttg taccggtcaa
     2641 caaggcatag atgatgcacg acaaacacaa acatgggttg ctcaacaact tcaaatacca
     2701 tctgagcatg ttgctgttgc ttcaactggg gtcattggtg aatatttgcc tatggataaa
     2761 attaagactg ggaccgaaca tattaaggat actaattttg caacgccagg tgcgtttaac
     2821 gaggcaattt taacaactga tacctgtaca aaacatatcg ctgtatcact aaaaatcgat
     2881 ggtaaaaccg ttacaattgg tggtagcgcc aaaggttcag gtatgattca tccaaatatg
     2941 gctaccatgc ttgcttttat aacaaccgat gcatcgattg aatcgaatac acttcatcaa
     3001 ttattaaaat cttcgactga ccatacattt aatatgatta ctgttgatgg cgatacaagt
     3061 acaaatgaca tggtattagt catggcaaat caccaagttg aacaccaaat acttagtcaa
     3121 gaccatccac aatgggaaac atttgttgat gcattcaatt ttgtctgtac atttttagct
     3181 aaagctatag ccagagatgg cgaaggcgca acaaagttaa tcagcgtcaa tgtgtcaggc
     3241 gctaaatcaa tatcagatgc aagaaaaatc ggtaaaacta tcgtaagttc aaatctagta
     3301 aaatcagcta tttttggcga agatgccaat tttggtcgaa tcattacagc tattggctat
     3361 agcggatgtg aaattgatcc taaatgcaca tatgttcaac tgaaccaaat acctgtcgtg
     3421 gataaaggta tggctgtact atttgatgag caagctatgt cgaacacatt aactcatgaa
     3481 catgtcacaa ttgacgttca acttggttta ggtaacgctg cagcgactgc atacggttgt
     3541 gatttatcct atgattatgt gcgtattaac gcatcatatc gaacataagg tggtgttggt
     3601 tagatgaaat ttattgtcat taaaattggt ggcagtacac ttagtgacat gcatccatca
     3661 attattaaca acattaagca tttacgatca aacaacatct accccattat cgttcatggc
     3721 ggtggcccat ttattaatga agcattatca aaccagcaaa tcgagccaca ctttgttaat
     3781 ggcctaagag tgactgataa agcaaccatg accattacta aacacacgct cattgcagac
     3841 gttaacactg cattagtagc tcaatttaac cagcaccaat gttctgcaat aggcttatgt
     3901 ggtttggatg cacagctgtt tgaaattaca tcttttgatc aacaatatgg atatgtcggt
     3961 gttccgaccg ctttaaataa ggatgcttta cagtatttat gtactaaatt tgtacctatc
     4021 atcaattcga ttggtttcaa taaccatgat ggagaatttt acaatattaa tgctgacacg
     4081 cttgcctatt ttattgcatc atcattaaaa gcgcctattt atgtattaag taatattgca
     4141 ggtgtactca tcaatgatgt tgttatacct caattgccat tagtcgatat tcatcaatat
     4201 attgaacatg gtgatattta tggaggtatg attcccaaag tgctagatgc caaaaatgcg
     4261 attgaaaatg gctgtcctaa agttatcatt gcatcaggaa acaagccaaa tatcattgaa
     4321 tctatttaca ataatgattt tgttggcaca acaatcctta attcataact atgaaattaa
     4381 ggcctaacaa gttttgacac gcgagatgat tccagttcga ttatccattg cgctaaaaca
     4441 tttatttacc gttcatctcg ttaacaattt tgaatacagt acgatacaat atgagatgta
     4501 aaaaactaat aaccttttac aaatttgttt atcaaaatat tttaagtttt gcaaagcttt
     4561 ttattgtgat tattttcaca aaatactata atgaggatag taaatagaga ggagtcctta
     4621 agttgacgaa acgacaaatg ggtatattca tttatgctgg aattatcggt ggcttgttat
     4681 ctggaattgt aaaattaggt tgggaggtca tgtttccacc tcgcacacca gaacgtaatg
     4741 caacgaaccc acctcaagag ttattgcaac aattaggatt tagtagtgag tttacgcatc
     4801 aaacatatac attttcaaat atggaattgc cttgggtaag ctttattgtc cactttagtt
     4861 tttctatcgt cattgcaatt atttactgca tattagttaa aaaatacgct tacttagcaa
     4921 ttggacaagg tgctgttttt ggtattgcta tttgggtatt attccacctt atcattatgc
     4981 caatcatgca tactgtacct gctgtgtggg atcaaccata ccaagagcat ctgtcagaat
     5041 tctttggcca tatcgtctgg atgatgacaa ttgaattagt gcgacaacat tttgtctatc
     5101 gctataaatt aaattaatac actgactaac attaacgtga gttttaaatc atcgtttgag
     5161 tatgatgatt gatgctcacg ttattttatt aactgacatg atatgattcc agccaactta
     5221 cgtgagcatt aaagtctcaa atgcgtcgta acaagatatt atttttggca atttcaatat
     5281 tgctcagtat attttcacct tatcacttac attaatctcg tcatgatttt gaatgatgcc
     5341 atcgtgtatt cacctttcat ttttccaata aaaatacatc taacagtaaa catttaggca
     5401 gtatagttta aaatcactgc gcaatgatac tgtcagacgt catattaact actcaataac
     5461 tgaaatacag acactttttt ataaccccag ggtgcctctc ctaagaaaca tacctgtacc
     5521 ataaactgat caaaaataaa ttgtttgaac ttcacttcac gtgattgata aaagtgtgat
     5581 tgtgtcatat cataaatgtc caatcctttg attaaacctt caccaatcaa ttttgtaaaa
     5641 ctttcttttt gtgtccatat ttgataaaaa tcatttaaac tacatatttg atgtgcttcg
     5701 tttgtagaga aacacgtcac taacgtacgc cagtctaaac gttgtgatat cttttcgata
     5761 tctataccaa ctggttcttt atcgacaaca cacacgatat aaggataact atatgataag
     5821 ctcacataga tgggctgtcc atcacgattg tgttgaacaa tatctgcctt acctcgtggc
     5881 gaaatgtgat aatgccattc atgtggtaat aaacctgtgt catgttgaat tccatattgc
     5941 actaaaatat ctcccaatct gtgcatgagt ttatcttgat tgtatctata gttgactgta
     6001 cgcggttttt tatatgacca acgactttgt gatattaatt cttcaatact tttcaagtta
     6061 ctctgtaatt gcattacaaa tactgtcata actttcccta cttacttatt gaatattgtt
     6121 ttgatatatt gtgcccaatg atacagccaa ttgttagtta tcgttggcca tttttcactg
     6181 atgtgattca ttatttttaa tgttaatgtt gtatctatca ttgctagttg ttgttcacgg
     6241 tcaacactag ttaatccaat cgtttcgtac atgtcttgtt tctgtaaaat ttcattaaat
     6301 gattcatcgc tgacgagttc aatttctttg cgcttaacgc attctaacaa agatttcacc
     6361 ggcattttat taggtgatag cacatggtaa atgatttgtg gtgtgttgac ctgtgctaat
     6421 gcgacaattt gtcttgcagt cgtatccaca aaagaaaaat ctacaggcat ttcagccatg
     6481 ctaaccccga tacaatccag ttgtaacaaa tcattcatta ccattgaaaa acggttagtc
     6541 tttatatttc tcatatgcca tcttccattg taaggactcg tcaaattacc aacacgtaca
     6601 atccgaccat ctaagccatt atttacagct tctaatactt ttaattcact ataaaatttg
     6661 ctccgtgtat atggtgatgt tagtagttgc cctttataga catccgcttc tgaaaatgtc
     6721 acatcttctg tgtctatatc aaaataagtt cccacactta tcgtagacac atatattaac
     6781 cttgcatgat gttgttgtgc caaacgtatg acatcaacag taccttgaac atttactttt
     6841 tcaaattcat catcatcacc aaagtgatcc gtacgagcac ctgcatgaat aatcgtatcc
     6901 atgttttcag gtaaaacaac atcatccata cactcgaaat caccaacaat gacttcaatg
     6961 tttgataaca tcatttcaac cgtctcttct gaaaaataat catttaaatt cgtcatcaac
     7021 ttataccatg ctatttcctc attatcagca cgtatgaaac aataaatgcg atgactgtat
     7081 ccttgtagtg cttcaatcag ataagcacct aaaaaacctg tcgcgccagt caatagtgta
     7141 tttcctagag gtcgatgact tagactatcc tctaaaatac ccaagttata acgagacata
     7201 acaatctttt gtaattccga aagattatcc ggtaatgcaa ctaatgattg ttgattttgg
     7261 tacatataat taacaatctg tcgcacggtt ttatattggt ataatgtctg cattgaaata
     7321 tgatggccaa atcgttttaa atgcgagaca actaacatcg cctctaatga gttaccacca
     7381 agttcaaaga aatcatcgtc aacaccgaca tcattttgtt tcaatacctc tccaaataca
     7441 tcaacaaatg tctgctcaat ttcattagag ggttcgctat acactttatt agactgttgt
     7501 ataggtgatg gatttggcaa acgcgtagta tccaccttgt cattcgtggt taatggcata
     7561 caatcgatat gcgttatagt cttaggaatc atatacttag gcagctgatc atttaaatat
     7621 tgcttcaaat cctgctccac ttgttgctct ccgacataat aagcattcaa tatatcatgc
     7681 gtatcaaagt gacttactgt tacaacacaa tcagatatac cacgaatagc taatattaca
     7741 ttttcaattt catcaagttc aatacggtac ccgttaactt tcacttgttt atctattctt
     7801 cctaaaaatt caatttgacc atcagatgta taacgtgcta aatcaccact atgatacaac
     7861 tttcctttac caaatggatt attttgccat ttatcagcca ttaattctgg acggttaata
     7921 tatcctatcg ctaaactatc acctgcaata cacaactcgc ctggcatacc aataccgcat
     7981 aacaaaccat ctgacataat atacacttgg atgttagata agggtttgcc aattggaatc
     8041 gtctcaggta tcaaatcacc acaatgatgt gaccaatacg atgtgatgac tgttgactca
     8101 gatggtccat aggcattgaa atacgtgcca caatgcttct caatatattt aacaaaggat
     8161 gccgtactag ttgccccgcc tgtaatcaac ttttcaatat aaaagtcttc cataatacta
     8221 cacatctgta acggaatcga cgcaaccgtc acacgatgct tattaatgag ttgttgtaac
     8281 tgttctggat taacacgttc ctctctatct ggaatcacaa gcgtatgacc atttaacaaa
     8341 caacaataaa tctccataac tgatgcatca aaaacaatat ttgcatgttg caaaaatact
     8401 tcattgtcgc ctaattgcaa ttcagttgac catgcatgca ctaaattcaa caaatttcgt
     8461 tgtcgtatgg caaccccttt aggcatcccg gtcgtaccag atgtgtaaat agcatacatc
     8521 tcattatcta acatcgctgt gttttcaagt tgattgccat gtaaatcatc atattgttca
     8581 ttttcctttg attcaacaaa gcctttagca ttttccaatt gaatgactgg taatgtcgtt
     8641 tcaatttcaa cgccgtaaga catgactgca gttactttag catcctccaa aattgcacct
     8701 tgtcgtttat tcggaaaatc aatatcgata ggtatataag atgcacctac tttaactgtc
     8761 gccaacatcg ccgcaatcat ttcaaaacta cgttctgtaa acaaggcaac ccgttgacca
     8821 ttgcccacac catttgatag gagcatgtgc gcaatggcat ccacatagtt gcgtaatgtt
     8881 tcatacgtca ttgtcaaatc attcatgact agcgcaacat gattaccttg tcgtgagaca
     8941 acttcattaa agtaacttat gatagattta tttcccggga cattaagcat tcgatcgtta
     9001 acatgcgtat tgacccaatt tagaagttcc tccgtaccgt ttggtatatc acaaatttgt
     9061 agtgtatctt gatgcttcaa aatataatca atcataatca tacattgatt acccatgtga
     9121 cgaactgttt ctgagtgata taaatcggta ttatactcga tattgattgt atagtcatcg
     9181 cgatcttctt caatgatgaa agataaatca aatttcgccg tcactgattt gggttgaatg
     9241 tgtgttaatt tactatgccc aaaatgagca tgattcgttt cattgttttg tagtactaac
     9301 atgacatcaa ataatggatt ccgtgaggca tcatgtgatt gatctaagtc atttactaaa
     9361 cattcgaatg ggtattcttg atgctcgtat gcctccaaac tcatttcctt aacctcttgt
     9421 aaaaactgtg tccacatttt atcaggtgac ggttgccctc tatataccaa cgtattagca
     9481 aacatgccta gcatttgctc cgtgccttta tgcatacgcg cactcatcac actaccgaca
     9541 acaacatcat cttttcgagc atatctgctt aacaacgtca tgaccacact cataaagaac
     9601 ataaaatcag taatttgatg cttttctaca tacttttgaa gtagctgtct catttgttga
     9661 ttcattgtaa atgacatcat tgctccattt gtcgttttaa tatttggtct aacatagtct
     9721 gtcggtaagc ttaaaatagg tacttcatct ttgaattgag ataaccaata ttgtctatgt
     9781 ttcgtcatat cacgatgcga catccactca ctatagtctt tatattgcaa tttaagtggt
     9841 aacaataatt tatgttgata aagtgcgtta agatcattca ttaattgtat attactcata
     9901 ccgtcattaa tgatatgatg tgtatctata aacaggtatg catgtaaggg acttctaatg
     9961 tatctcactc taatttgact tggcttttcc aaattaaaag gtgccacaaa ttggcgcata
    10021 atttcttgtt catccgtaaa atgcgtgtta acttcttcaa agtcagctac aacatctgcc
    10081 acaatacgtt gtcgaacctc atcatctaca acaatatatt gtgttcgtag aatctcatgt
    10141 cgcgctatca aatgctgaac tgcttgttgc aattgagcta cattaagtcc tgatgataac
    10201 cgccataaaa aaggtacgtt atacaccgta tctttatggt ttgatttcca taataaatac
    10261 atacgctttt gtgcagagct cagcacataa tcatctttaa ctatagcttc tggaatcact
    10321 tcatagtttt gttcttgaac cttagcaatc gcttgtgcta gttcaaatac agttggcttt
    10381 tgtaataaat caccaatttg taatcgtttc ccagtagatg cctctatccg attcaccact
    10441 aacgttgctt ttaatgaatg gccacctaat tcaaagaaat tatcatgaat acctacttga
    10501 ttcacatgta aaatatctgc aaaaatttgg catagcaagt gttcggtatc tgtactcggt
    10561 gctacatagg catccgtatc gacatagtcc atgataggca atgccttctt atctaatttc
    10621 ccattaatag taataggaat ttgctcaata tgcatgaaat taactggtat catgtactcc
    10681 ggtaaggtca tacgtaattg tgatttaatc ttattatgtg ataatgtatg catcgcttca
    10741 taataagcaa cgatatactg atcttgatca tgattttgaa caataacaac tgctttatta
    10801 ataccttgta tacgctcgag cgcatgctca acctctgaca actcaatcct aaaccctcga
    10861 atcttaactt gtttgtcctt tcgatataaa taatctatgt tgccatcggg taacaaacga
    10921 acgatatcac cacttctata catcagctga tttatatttg aatctttgat aaatttatct
    10981 gctgtcaatt ctggctgatt taaataacct gcagctaacc caaagccact tgtacataat
    11041 tctccaggaa taccaacgcc acaccgacgc tcgccttgca tgatataaac atgagtaccc
    11101 agaatcggtt taccaatagg aatacgattt ggaactttgt taggtatatt atacgtcgtt
    11161 gtaaatgttg tattttcagt tggtccataa ccattaataa tttgaggatg cttcggtttt
    11221 tgattaagca aatccaccca cttagcattc aatacttctc caccaattaa taaatacttt
    11281 aacggtacca atacttctat tcgttcacta gcaatctgat taaataatga ggaggttaac
    11341 cacatagtat taacgtcatt ttcattgatt aattgttcta ccgctattgg atttaataat
    11401 tgttcttttt tagcaacaat cagctttcca ccattgagca atgcaccata tatttcaaat
    11461 gttgcagcat caaaggctat agttcctgat aacaaaattg tcgtctcttc atttaatggt
    11521 acataatgat ttcgatggac caagcgaaca atacctcggt gcggaattag tgtcccttta
    11581 gggttaccag ttgtccccga cgtgtaaata acataagcat gatcttctaa cgtgttacat
    11641 ttagaaagat tatcaatatt tttccacgct atcttattca aatcaatgtg attaatattt
    11701 tgtttaccat tttcatataa agcttggtac gttattacaa ctttaggcgt tgcatctttt
    11761 aaaatgtact cctgacgatc acttggatag ttcggatcaa ttggcacgta agccccacca
    11821 gctttcaaca cacctatcat cgctattatc atctcaatac ttttttcagc tatgacagcg
    11881 acacgatcat taggttcaac accatactgg tttctcaaac ggtgtgctaa atcattcgcg
    11941 cgtgcattca atgtttgata tgttataaac actccgtcaa attgcacagc gacatgattc
    12001 ggcgttgctt caacttgttg ctcaaataag gtaacaactg tttgcgcatc atctatctca
    12061 ggcaaactta aattgatatc gtcatataat tgaatatcac gttctgtcat caaattaagt
    12121 tcatctacag ttgttcgttt atttccattt tcttcagtaa tttgcaaata aatatttcga
    12181 actaagtcac tcagcgtctc gattgagagc aaatcatatg ctgcactgtt atatataatt
    12241 ttgaacccgt gttgatgtgg aaaaatttca atatccgcta aagatgtatg tgcatcatgt
    12301 atttgatgta catcctcaat aacatcatta caacaagaca tcatatgatg ataacaatga
    12361 aaaatagtct ctagtgaaag cgaagacttc gcgcactgta attgcgacat attttgcaac
    12421 acacatttat taaaatctgt tgtaaaacgt tgacatacat cttttgcatc gattgttaac
    12481 gttaacggca caatatttcc gtgtaaatca tttggtaaat gtgatggtac atgtatacct
    12541 aatgtgacat catgttgttg actcattata tgattagcta aatacacact aacagccaac
    12601 gatgccatat ctattgatgt catatcatca atcaaatacg tttgatataa agcttgttca
    12661 aatggatgct taattggaaa ataactatca atatggatgt cagagttatt ctctaaccga
    12721 aaatagtttg agtctaatgc tatatgcgat gcatcttgat tgtctttatc atcatttcta
    12781 tttatatgtg catgctgtcg agtattgtta ataacagtat tgccacgata tgcattgcat
    12841 aaatcatcaa gaaaaatatc aatttgacta tcatcgaaaa tggacacatg aaaatctaat
    12901 agtatatatg cagcatcagc gaactgaaac aatttaactt tgaataaagg tgaatcatta
    12961 aaatggtaag tacttaattc ttgcttaaaa aaagcttcta aatcatagtt tgcggaagaa
    13021 gatggaactt gttttatctc aataaaaggc agaaattcat gaagtatcat tgttaaattg
    13081 tcatcggtag taacatcaaa aaaatgtctt atagatgcat gttgttgcac aattgtcgat
    13141 aatgcataca tcattttagt agcttcaaca tttttagcga gtttaaccca atacgcatta
    13201 cggtgtgtcg ttgattctgt attatttttg tatatacgaa aatattcctg ttgaaatctc
    13261 aaattaccca taatcataaa aagtccttct ttcatatcat aatactcatt acttactaaa
    13321 attgcatgat gatatgataa ccgacgaaat gttaattaac tcgttatgta atgattaata
    13381 taaaacacca ttcgcaacat atgagcgata tattctaccc taaaatacat cttgtatcat
    13441 cgttacaatt ggtatatttt tcaatgtaaa ttacatacat cttcgataaa tagcacacta
    13501 caactcgtta atcactttct gttgttcata tctcattgca aattcaatat tgttgttaca
    13561 aaatatccat gaagcaagtt tatattaaac aaacaactcg cataaaacaa ttgttatcct
    13621 taaattttaa caaattctta ataaatttat ctctatttta attacgacca aattaatagg
    13681 ttttcaatat aaaaagatgc ataaaataaa tatttaaata aattcaattt gtattcactt
    13741 gtttttgtcc cccgaataca ccagcaacaa gcatgctagc accaattgtt aaaacgataa
    13801 acatatacag tcccatttgt aatgacgtta agaaaacacc caacacaatc cctaacctag
    13861 ctagtgtttc tgaaaaatga atacctaatg cattaactgc actatatgtt cctcttttag
    13921 ctttaggaat aattttaaag cgttgttctg aaactatagg cgaataaata atctcaccta
    13981 cagtcgcaat tatcataaaa acaactaata agccaaactg attaagatag gttagaccac
    14041 tatagccaac aatataaatc agcaaaccag tgattaaagc ttttttaaaa tctattttca
    14101 atacaacttt cgagattgaa tacgtgagta aaatgacgac gaccgtatta atcattagca
    14161 agattgctaa catcttagca cctgtaatat catatgaacc tatacttatt gtttcaaact
    14221 gatcctttag tctaatagca atatatgagg agattgaaaa ttcacccatc atgatgatac
    14281 tgaaccccga aatcaataac atataattac ggtctttcaa aactaattta taactgcgaa
    14341 atatattcat tatttgtaat ttttgataac gacttgcatg cctcttgtca tcactttgct
    14401 ttacttgatt tcggtcttga ggtaaccaaa tatataaaat aaagagtaca attaaaaata
    14461 tacaagctgc tattaagaaa agtagtaaca tactgtagcc atacatcaag ccacctaaca
    14521 atgccccaat agctaccgat aagttcgtca tccaatagct aatcttgtaa atataatgtt
    14581 ccacgtcttc ggtaattgca tccataatta atgtgtccat aactggaaat tgtaatcccc
    14641 aaacgattgt aaatatggca tatgcaacac aaaaaccaat aatttgccac aattgatgtg
    14701 acccaaatac gcccatgaac acaagcatta tcaccatcgt cgcttgataa ataagtacta
    14761 gcaacttttt cggaaaaatc tcaataaggt aaccagatat aatggacaat ggaaatttta
    14821 gaaccactaa accaacaaga tatataccga caattgattg acttaacata tctgttaaat
    14881 atagtgctat aaacggtata aatgctgtcg taataattag ctgtaaaaaa ttgctaatca
    14941 atcgtacttt caaagtcgta cttaagcgtt tcattaataa ctacacccct atccacatat
    15001 atacacacta ccatttatat aaaatctatt attagattat acgttgaaac aaagaatttt
    15061 tagttataca aagttattta acaaacaaat gcatcgattt tacaatgcat cttgcatata
    15121 atgcctcaaa aaaattagca ctatatgact atatattatt ccaaatgaat gaaatttatc
    15181 aataattgcg taagtgaaca catatgtgcg taaatgttaa atcattgtcc taacatagcc
    15241 gtgactatac ttagatgtaa tcataaaaaa actactgcca tctttacaac agcagtagtg
    15301 cttatatgat tcatttatat aaaattgaaa gcgttaatcg ctagcccagc atccttattc
    15361 tatttagctg tataactttt actagcaata cgaccacttg caacaataat atctttatct
    15421 tggaaaggtt catgattgaa gaaacgctct aaaatatctt taactccatc ttcaatgcgt
    15481 ttttgtgctt ctaaagtcat acctgaatag tgaaccgtca tagcatttct aggcattgtt
    15541 ctccatggat gatcagcagg tgcaggttgt ggataccaaa catcaccagc atatccttgt
    15601 aaatgctcgg atgctaacgc ttcaactaac gcatcgcgat ttacaatttt accacgtgca
    15661 gtattcacta aataactgtg ttttttcata cgacttaaaa catctttatc aaataagtta
    15721 tcagtttctg gtgttaatgg tgcatgaatt gtaatcgcat cacttgatga aacaagttca
    15781 tcaaagctta caaatttaga caatttatgg tcttgttgat tgattggatc atagtgttgt
    15841 aatgttacat taaatggcgc taatctttca gcaacaagtt gtccaattcg accaaatcca
    15901 aaaataccaa ttgttttgtg ttgtaattca tgcgcatgat tacctacttg agacaagttc
    15961 cattcacctt ctactgattg acgatgacct tcttcatagt ttctaagaag tattaataaa
    16021 tccataaccg catgttctgc cacactaact gtattacttc ctgtaacttc aacgacacca
    16081 atattgtgtt cacttgctgc cgctaaatct acatggtcag atcctacacc tgctgtaatt
    16141 gctaatttca agttcggtgc tttttcaata cgttcacgag tcatatatgc aggataaaat
    16201 ggcgcactaa taatcacatc catatctggt aaatgtttat ctaagtcttc accattatct
    16261 gctaatataa tgaactcatg tcctctttcc tctaaaaatg tttttaatcc tattgctttt
    16321 ttagtattaa gtaattgatt ttcttgacct tctactgctt ctggaaataa tgctacgatt
    16381 ttcatatcca tctctcctaa atgataaatt ttgacttcct taaagtacgt gttgcatgtt
    16441 gcttgtttta aaaatatgac aaaaaaaacg gcaccgtttg acataccacc cccttgaatt
    16501 attgttaaat tcaaagcgaa gttagcaaac agcaccgtgt accttacacc agcaacactt
    16561 attctattga cttatcgaaa acaaatattg aaatgacttc atcatcattc aaatcaatat
    16621 ctgtaaataa ttttacaaac ttagcgccta caagactttc catctcattt acatctattt
    16681 ctttatacat ctgtttaatc ttctctgtgc gtgtataatg gagcattgat tcattgcgtt
    16741 tgtcgtttag gtaaaaactc tcaactttac tcaaaacacc tgtcatatgt gcaatcgccc
    16801 aattatcttt aaacgacact cgaatactat tgggtccttt acccacgtat tctttacgat
    16861 aagatcttac tagattcgag aattgttgtt ctttagtatg cgtatctata gtattcacct
    16921 tctttaaaat aagaagttca ttaattgttc acattttcat tatacacctt attaacactt
    16981 tttcaatgat aacgattatt attattacga aatgtatatt ttcctagtta aaaggatata
    17041 acaaattaac agaaaatgtt tataaactaa gatttatatt attttaaaaa atgtttcaac
    17101 aaacgcatct aaacaaaaaa tcagcttatc acacttaccc cttcaactct gcttcaagtt
    17161 tacctaaatg tcttaatgtc ggcgttaatg ctgcgaagaa gaatccttct tttaacttgc
    17221 gaacttgtgg cgaatatggt gtgtacgctc ttgaaccacc attgacgaca gaagcttgat
    17281 ttacatctaa caataaatag ccaatgtcct tcttcaatga tattaattca cttaaatgtg
    17341 aagttaagtt accgtcatct aatattgcat aatattcctc tctaagttga cgataacgtt
    17401 ttttaaaagc ttcaacatca tactctaaat attgatttat tccgttttgc gcatttgaaa
    17461 atgcatcaat taactctaaa gaacttttaa ttgagcctaa tcctattgga atttggtaag
    17521 caataaattg cgggcgaata gttgccgcaa actgcttcgc atcatgcgtg atgatttgtg
    17581 attgtggcac tacgacttga ttcaatgtga tttgatacgt tgctgacccg ttgaccccta
    17641 aaaaattggt tttttcaacg agagtgatac catcttgatt ggcacgtaga atgaacatga
    17701 caaattcatc tgatgattca tgtttcgaaa tcgcaccaaa ataatggtct tcttgaatat
    17761 tacttacagc tggcatacgt ccactgacaa ccaattgtcc atcaacataa gtgtgttcaa
    17821 ggttcaactt ttctaaatca ttaaatgact tcatcggatt agacaatccg gtagcaccta
    17881 atatttctcc agataacaat tgctgttgta agtcattatt taaatgtggc tgcgtggcat
    17941 tttctaaata cgttgaaaaa gctaattggc accataaaca aaatcctgtt gtcaaacaag
    18001 actgcgatac agcttcgatt acttcagcat tttttctcaa tgccgcctca ccgaaataac
    18061 catctacaaa taattgttga ataaatgttc tcggataata cgtcccttca tcaacttcta
    18121 ctaaatgagg atctaattgt tctttgataa gcgtttctaa agtcatcatc atctctcctt
    18181 attcaccaaa tcttctaagt ataaactgct caatataact aatgaatcga tcaataataa
    18241 aaccaaataa tccgataaag aatattgctg ctaaaacatc ttctaagttc aacatatttc
    18301 gtgcatcaac gattaaaaaa cctaatcccg attgtgcacc aatcatttca ccagaaacta
    18361 aaaatatcca acttgttcct accgccatat gtatcccagc catgatttgt ttaaatgccc
    18421 cgggaaataa tatattgcga tacaatgacc acccagttaa atttaaattt gccgctattt
    18481 ttaaatattg aggttcaatg tctctaacgc ctttaatagt attgaacaca attgggaaaa
    18541 aagcagcgat aaaaataatc gcaatcgctg gcaaactacc aataccaaac catagaacaa
    18601 caaatggtgc ccatgctatc ggagatatcg gtctaatcaa ttgaaatagc ggttcgatag
    18661 cgttgtatag ccaacgattc cttccaagca agaagcccaa tggaatagca accaacaatg
    18721 cgacaacaaa gcccgctaca aatctccata aactaattgc taaatgttgg aaaatttctc
    18781 cagtaacaat gaaagtccat atactttttc ctacaagagc aggaccaggt aacaatacag
    18841 gttggtaatg cccaataata atgaccattt cccaaatgcc taagaaaata ataaatgtga
    18901 taataggtaa tataaatttg ttatttgtgg gacgtgtcat gaacgcgatg cctccttata
    18961 caatgacggt tcaacaaagt catcatatgc aggtggatta aacaaatgat gttgttttac
    19021 caatgtcgta atttcttgat agccggatgg cttaattgtt aaatcaccat aggatgtcca
    19081 tgccgctgac tgtgttaaaa cgtcacgact ttgtttaaaa tgatgcgtca taatgtctac
    19141 actttgcttg cgatcattca ttttaaagcc agacttttta taatcttgta caaatgcttg
    19201 cgctacatcc ttgtgttgat caagcaattc ccctctcagt actagcacac agcaatacgc
    19261 atcaggtata acgtcatcac catgtttcaa agtcttacct ttgcctaact tttcacccag
    19321 tgcaccgaat ggttcggcta cagaataccc tgtaattctg tgttcactca atgcggctgg
    19381 catttctgct ggcgacattt catgatagct aaaatgcccc ggtttaatct ttaattgttt
    19441 acgtaattcc tcaagtaaaa gataatgtgt tgaataacga tgtggtatac caaaatggta
    19501 atcatcgcca ttattaaatt catttaagtg catacctttt tgtcccataa tgacattgcc
    19561 ttcatgatgg cccaatgcca cagcctttat atttgagccc ttctgttttg atttcatcgc
    19621 tagctctatt aaagttgatg caccatcaat acgaccactg tttaatgcgt ccattaaatc
    19681 tggccaatta ttgaatttaa ctaattctag tttatatttc ggatgattgt attgtgataa
    19741 taatttttta gtcatcatca aattagctga atgtgtaatc ggcaaatatc caattttaat
    19801 cacttgctga ttttgggcat ttttagacgg ttctttagac gtcctttgcc aatcacatcc
    19861 tgtaattata aagattccaa tgatgacgat tatgcttaac cttttcatcg tcactcactc
    19921 cttataaata atattcaggt tcaacttgat gatgattcaa tgcaaatgtt tccataattt
    19981 cattacgaat cttaagtagg tggctatcat tacgactgcg tggatgtgat gctgtaattt
    20041 catattgaga aataatattg cacccttcac ctaacagaac aatgcggtcg gaaagataaa
    20101 tagcttcatc aatgtcatgc gtcactaaaa taatagttga ttgcgtttta tgttttagtt
    20161 gcactagttg atcctgaagt ttataacgtg taaatgcatc taatgcacct aatggctcat
    20221 ccatcaatat aacgttaggc ttatgcacat gcgctcgaca tagtgccaca cgttgtttca
    20281 tacccccgga cagttgctcg ggaaaatgct ttcccctgtc ttctaaatca actaatttaa
    20341 gctgtgcgtt aatctcttca tcactaattt tctgttgaaa tccaatccta atgttgtcat
    20401 taatcgtttt ccatggcagc aaattatgat gttgaaatag cattaaacaa tctggagatg
    20461 gctgttgttt aatttcgtta tcaataatga cacgaccaga cgatggatga ataaatccac
    20521 cgataacatt gagtaaagta gactttccgc aaccactttt ccctatgaaa gtgactattt
    20581 ctcccttgct aatgtccaaa ttaaagttat gaattacttt atgtgatcca aagtgatgtt
    20641 gtaattgttg aattttaatc atgacaagcc cccttttaat ccctactatt ttaataggaa
    20701 ttaagtgtat atctaaaggg ctatgccaaa cacagccctt atatcgtgaa ttgttatatc
    20761 tatagatgat tatggtttgg tatgagtgct cttataacat aagcgttatt caattaacga
    20821 cgttatatta atttcagctc tactaaattt caataaaatg attgttttag aaaatacttg
    20881 catttagttc attagtttct tcatatcaac tacttacatt tatagcattg agctattcta
    20941 attaactgca atcgaaatcc acgtcaaacc tatatacata tatctcatca acttgttgtc
    21001 ttattaaagt ttttcgatgc catcaatcgg tgtatcaact gcttttcgcg cgcgacgtac
    21061 cgcttcttca tcaggtgcgt tgtataaaca aatacattta gacatatctt cacatacata
    21121 tgtgcgttta aattcaactt ctggcacttc ttcataatga acagaatttt tctttttacg
    21181 tgctaaatat tgatccatcg taatgccttc cggaatgttc caagttacaa ggtaatcaac
    21241 atgtgcatca ccatttttaa cttcatctaa atctttacca actaagcgta cttctttgac
    21301 taattgaata ctaatatctg cttctgttaa aaattgttta gcttctgctt cgtcatttgc
    21361 ttccacaata aaataaccat gagttaaatt ttcagtagct tgtacttcaa ttaatgttgg
    21421 aattgtcttc gtagatagtg atgctgcctt tgcttctagt tcttctttcg ttgatgcaaa
    21481 atcaagattg ttagcttcta ataaaaataa tgtcattaat aagacctcct ataacaatgg
    21541 ttgaaaa
//
LOCUS       DAHHSC010000024        21208 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.024, whole genome shotgun sequence.
ACCESSION   DAHHSC010000024 DAHHSC010000000
VERSION     DAHHSC010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 21208)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21208)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21208
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.024"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            225..3677
                     /locus_tag="NBI57_000708"
     CDS             225..3677
                     /locus_tag="NBI57_000708"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HCD1821674.1"
                     /translation="MKQIKKLLVANRGEIAIRIFRAAAELDISTVAIYSNEDKSSLHR
                     YKADESYLVGSDLGPAESYLNIERIIDVAKQANVDAIHPGYGFLSENEQFARRCAEEG
                     IKFIGPHLEHLDMFGDKVKARTTAIKADLPVIPGTDGPIKSYELAKEFAEEAGFPLMI
                     KATSGGGGKGMRIVREESELEDAFHRAKSEAEKSFGNSEVYIERYIDNPKHIEVQVIG
                     DEHGNIVHLFERDCSVQRRHQKVVEVAPSVGLSPTLRQRICDAAIQLMENIKYVNAGT
                     VEFLVSGDEFFFIEVNPRVQVEHTITEMVTGIDIVKTQILVAAGADLFGEEINMPQQK
                     DITTLGYAIQCRITTEDPLNDFMPDTGTIIAYRSSGGFGVRLDAGDGFQGAEISPYYD
                     SLLVKLSTHAISFKQAEEKMVRSLREMRIRGVKTNIPFLINVMKNKKFTSGDYTTKFI
                     EETPELFDIQPSLDRGTKTLEYIGNVTINGFPNVEKRPKPDYELASIPTVSSSKIASF
                     SGTKQLLDEVGPKGVAEWVKKQDDVLLTDTTFRDAHQSLLATRVRTKDMINIASKTAD
                     VFKDGFSLEMWGGATFDVAYNFLKENPWERLERLRKAIPNVLFQMLLRASNAVGYKNY
                     PDNVIHKFVQESAKAGIDVFRIFDSLNWVDQMKVANEAVQEAGKISEGTICYTGDILN
                     PERSNIYTLEYYVKLAKELEREGFHILAIKDMAGLLKPKAAYELIGELKSAVDLPIHL
                     HTHDTSGNGLLTYKQAIDAGVDIIDTAVASMSGLTSQPSANSLYYALNGFPRHLRTDI
                     EGMESLSHYWSTVRTYYSDFESDIKSPNTEIYQHEMPGGQYSNLSQQAKSLGLGERFD
                     EVKDMYRRVNFLFGDIVKVTPSSKVVGDMALYMVQNDLDEQSVITDGYKLDFPESVVS
                     FFKGEIGQPVNGFNKDLQAVILKGQEALTARPGEYLEPVDFEKVRELLEEEQQGPVTE
                     QDIISYVLYPKVYEQYIQTRNQYGNLSLLDTPTFFFGMRNGETVEIEIDKGKRLIIKL
                     ETISEPDENGNRTIYYAMNGQARRIYIKDENVHTNANVKPKADKSNPSHIGAQMPGSV
                     TEVKVSVGETVKANQPLLITEAMKMETTIQAPFDGVIKQVTVNNGDTIATGDLLIEIE
                     KATD"
     gene            complement(3817..4728)
                     /locus_tag="NBI57_000709"
     CDS             complement(3817..4728)
                     /locus_tag="NBI57_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000467197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme A synthase"
                     /protein_id="HCD1821675.1"
                     /translation="MFGKKNLKWLGVVATLMMTFVQLGGALVTKTGSADGCGSSWPLC
                     HGALIPEFFPIDTIIELSHRAVSALSLLMVLWLVITAWKHIGYIKEIKPLSIISVGFL
                     LLQALIGAAAVIWQQNDYVLALHFGISLISFSSVFLITLIIFSIDQKYEADELYIKKP
                     LRRLTWLMAIIIYCGVYTGALVRHADASLAYGGWPLPFHDLVPHSEQDWVQLTHRIMA
                     FIVFTIIMITYIHAVKNYPNNRTVHYGYTAAFILVILQVITGALSIMTNVNLIIALFH
                     ALFITYLFGMTTYFIMLMLRSVRSDKQ"
     gene            5168..6079
                     /locus_tag="NBI57_000710"
     CDS             5168..6079
                     /locus_tag="NBI57_000710"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX farnesyltransferase"
                     /protein_id="HCD1821676.1"
                     /translation="MSKEHTLSQNISRVNFKELQQIIKMGLVQGNLIPAFAGAWLAVV
                     MTNHSFLSSIPQILLMLFGSTLIMGGACALNNYYDQDIDRIMPSKQNRPTVNNRITDQ
                     NLLLLSFGMMLVGEICLFLLNIPSGVLGLMGIVGYVSYYSIWSKRHTTWNTVIGSFPG
                     AVPPLIGWVAIEGQISLTAIALFLVVFCWQPIHFYALAIKRKDEYALANIPMLPSVKG
                     FKRTRVSMFIWLIILLPVPLLLINLGVVFVVLATLLNLGWIALGLTTFKKNSDQTKWA
                     TQMFIYSLNYLVIFFVLAVIVSLLTLI"
     gene            6104..6565
                     /locus_tag="NBI57_000711"
     CDS             6104..6565
                     /locus_tag="NBI57_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF420 domain-containing protein"
                     /protein_id="HCD1821677.1"
                     /translation="MGVPILPTISTTCIVISAILIAIGWRLIWKREINKHKNVMLAAA
                     VFALTFFLIYASRTIFIGNTAFGGPASIKKYYTIFLFFHINLATIGGILGLVQIITAF
                     KDKYNVHRKFGPFASVIWFCTAITGVAVYLLLYVLYPGGETTSLIKATFGH"
     gene            6892..7929
                     /locus_tag="NBI57_000712"
     CDS             6892..7929
                     /locus_tag="NBI57_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499615.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HCD1821678.1"
                     /translation="MKKLIIRVVGVLFLVGFLIYLFYSPRLKFDVLENPNKGNKVNRS
                     EQVNKSNNHAENPKPKEGVGTWVGKDIKVLTSKFGQADRVYPFRDGYKNYVFKDKNSY
                     YIVSTKREEIVSVYATGEKVNVSPLKIGQHSAEIFNHTSINPEPSFKVDGKKYEFELS
                     DEDLKTQTLIKYGDIYAQVYSDQQSKKVLSVRFLTKEMLADIEPYQLNSNSTSEEHNK
                     RPVEQNPNQLISLYEVTNEMRKLKGLKPLKINSDLAHIASNNLYEATSNGSDSVEFTE
                     DALRGQLDKNHVTYKTTAQNVGYAFNDVPTLIHSWMNSDIHRSRLLNSKYDEMGGDVM
                     RDYYSLIFLEK"
     gene            7945..8379
                     /locus_tag="NBI57_000713"
     CDS             7945..8379
                     /locus_tag="NBI57_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HCD1821679.1"
                     /translation="MINEASLAILDDIDELADMIVASDIYASFEQAKQALENNDEAHL
                     LYQSFLKSKEKYDEVMRFGKYHPDYKKVMLETRQRKRAYEMLDVVMHYKAREMALQHL
                     IDEVVTKIAYAVSEHVKIETGNPFFQTSHSGCATGGSCNCSL"
     gene            complement(8442..9368)
                     /locus_tag="NBI57_000714"
     CDS             complement(8442..9368)
                     /locus_tag="NBI57_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HCD1821680.1"
                     /translation="MKKVNRNILNAVLVGAGFLSSLLMINKNKVITKKQTIPAFFKGN
                     APYIFAHRGGMALRPEQTQLAFDYAKQLGVDGFETDVRLTKDQQLIVFHDATVDRTTN
                     GSGKVSAHTLAELKKLDAAYHFKDINGLTPYRGHAHTTILTFDELLKQYPDMYINVDL
                     KDAPESYEGSIAPQIMFDTIAENQAFDRVLVTSFYKEQIVRFNKIAQGSVAIGASQQE
                     VTEAFLKYHLLGGRYYQPLAQTFQIPTHFKGIDLTSSRFIKWLNDMNIIPGYYGVNSI
                     DLMNDLYQKGAHTIVTDRPDLAQQFKQTIPNK"
     gene            9607..9861
                     /locus_tag="NBI57_000715"
     CDS             9607..9861
                     /locus_tag="NBI57_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HCD1821681.1"
                     /translation="MNLIPRTSIVVYLKHMKHERQIRKYGHIVHSNRDRKFVIMYVNE
                     QDVDQIVHKLMQLKYVRHIDGSPYKYLKKTYEKEKHEIYN"
     gene            complement(9864..10253)
                     /locus_tag="NBI57_000716"
     CDS             complement(9864..10253)
                     /locus_tag="NBI57_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1821682.1"
                     /translation="MKQTFITLGEGLTDLFEFMTMIEYNHQRIDKIIYFHSPQAENKK
                     SSVAIIMNPTTGNHFQAFYIMINAIKYPYPDSNKKFQMINDCAEKFDIPILGIDVQPP
                     QAFHDLSLYYNYLISVLRLQKWIPELQ"
     gene            10323..10865
                     /gene="rsmD"
                     /locus_tag="NBI57_000717"
     CDS             10323..10865
                     /gene="rsmD"
                     /locus_tag="NBI57_000717"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002470236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HCD1821683.1"
                     /translation="MRVIAGKHKSKALESMEGRNTRPTMDKVKEGIFNSLYDVSGIGL
                     DLFAGSGALGIEALSRGMDKVIFVDQNFKAVKVIKSNLANLDLEAQSEVYKNNADRAL
                     KALSKRDIQFDVIFLDPPYNKGLIDKALKLISEFNLLKENGIIVCEFSNHEEIDYQPF
                     NMIKRYHYGLTDTLLLEKGE"
     gene            10867..11349
                     /gene="coaD"
                     /locus_tag="NBI57_000718"
     CDS             10867..11349
                     /gene="coaD"
                     /locus_tag="NBI57_000718"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HCD1821684.1"
                     /translation="MEHTIAVIPGSFDPITYGHLDIIERSTDRFDEIHVCVLKNSKKE
                     GTFSLEERMDLIEQSVKHLPNVKVHQFSGLLVDYCEQVGAKTIIRGLRAVSDFEYELR
                     LTSMNKKLNNEIETLYMMSSTNYSFISSSIVKEVAAYRADISEFVPPYVEKALKKKFK
                     "
     gene            complement(11411..12550)
                     /locus_tag="NBI57_000719"
     CDS             complement(11411..12550)
                     /locus_tag="NBI57_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HCD1821685.1"
                     /translation="MKSVGLITEYNPFHNGHQYHINQSKKLTNADVTIAIMSGNFVMR
                     GEPAIYNKFTRAKMALSTADLVIELPATASLSSGDHFAELAVKVADYMSVDTIAFGSE
                     NNDIKTLKQLAHSINEIEQSESFSQKVKEGKSYPRIISELLEHHEALASPNNILGISY
                     LKAIAKNAKNINAISIKRENAQHHDSLIQHHQFASGTSIRTSIISQDDHWHHVVPKDI
                     QHLYVTPHITLNQIFPYLKYQIIAMTTDSLKNIYTVTEGFENRLKSNIYEATDFHHFV
                     KLLKTKRYTYTHIQRLLMNVLLNIKPTDVTSNIHAVKVLAMNDRGRQYLKHLKTAFPE
                     RQYITNINKSNAHYFTNEIKATHIYNAISGQQQTDFNTPVIQQYR"
     gene            12677..13234
                     /locus_tag="NBI57_000720"
     CDS             12677..13234
                     /locus_tag="NBI57_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HCD1821686.1"
                     /translation="MKWSITQLRKYQGKPFEFDQTVSFDNLKESLDLIDLSPITIQGQ
                     LTIKSTEVVADIHITGTYTMPCARTLVPVKVPLDVTTTEVFDLEGYNQYNDDQDDVDE
                     HYHIIKDGMVNLQDIVEDIVIIEKPMRAYSEQSDQMLTVGNGWEVIDEDQLDELAKQQ
                     EQDDSESRQVDPRLQKLQQLYDKEQ"
     gene            13314..13487
                     /gene="rpmF"
                     /locus_tag="NBI57_000721"
     CDS             13314..13487
                     /gene="rpmF"
                     /locus_tag="NBI57_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019469283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HCD1821687.1"
                     /translation="MAVPKRRTSKTRKNKRRTHFKISVPGMTECPNCGEYKLSHRVCK
                     NCGSYNGEEVAAK"
     gene            complement(13641..15578)
                     /gene="isdB"
                     /locus_tag="NBI57_000722"
     CDS             complement(13641..15578)
                     /gene="isdB"
                     /locus_tag="NBI57_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme uptake protein IsdB"
                     /protein_id="HCD1821688.1"
                     /translation="MNKQQKEFKSFYSIRKSSLGVASVAISTLLLLMSNGEAQAAAEE
                     TGGTNTEAQPKTEAVASPTTTSEKAPETKPVANAVSVSNKEVEAPTSETKEAKEVKEV
                     KAPKETKAVKPAAKATNNTYPILNQELREAIKNPAIKDKDHSAPNSRPIDFEMKKENG
                     EQQFYHYASSVKPARVIFTDSKPEIELGLQSGQFWRKFEVYEGDKKLPIKLVSYDTVK
                     DYAYIRFSVSNGTKAVKIVSSTHFNNKEEKYDYTLMEFAQPIYNSADKFKTEEDYKAE
                     KLLAPYKKAKTLERQVYELNKIQDKLPEKLKAEYKKKLEDTKKALDEQVKSAITEFQN
                     VQPTNEKMTDLQDTKYVVYESVENNESMMDTFVKHPIKTGMLNGKKYMVMETTNDDYW
                     KDFMVEGQRVRTISKDAKNNTRTIIFPYVEGKTLYDAIVKVHVKTIDYDGQYHVRIVD
                     KEAFTKANTDKSNKKEQQDNSAKKEATPATPSKPTPSPVEKESQKQDSQKDDNKQLPS
                     VEKENDASSESGKDKTPATKPTKGEVESSSTTPTKVVSTTQNVAKPTTASSKTTKDVV
                     QTSAGSSEAKDSAPLQKANIKNTNDGHTQSQNNKNTQENKAKSLPQTGEESNKDMTLP
                     LMALLALSSIVAFVLPRKRKN"
     gene            complement(15781..16833)
                     /gene="isdA"
                     /locus_tag="NBI57_000723"
     CDS             complement(15781..16833)
                     /gene="isdA"
                     /locus_tag="NBI57_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored heme-scavenging protein IsdA"
                     /protein_id="HCD1821689.1"
                     /translation="MTKHYLNSKYQSEQRSSAMKKITMGTASIILGSLVYIGADSQQV
                     NAATEATNATNNQSTQVSQATSQPINFQVQKDGSSEKSHMDDYMQHPGKVIKQNNKYY
                     FQTVLNNASFWKEYKFYNANNQELATTVVNDNKKADTRTINVAVEPGYKSLTTKVHIV
                     VPQINYNHRYTTHLEFEKAIPTLADAAKPNNVKPVQPKPAQPKTPTEQTKPVQPKVEK
                     VKPTVTTTSKVEDNHSTKVVSTDTTKDQTKTQTAHTVKTAQTAQEQNKVQTPVKDVAT
                     AKSESNNQAVSDNKSQQTNKVTKHNETPKQASKAKELPKTGLTSVDNFISTVAFATLA
                     LLGSLSLLLFKRKESK"
     gene            17042..17725
                     /gene="isdC"
                     /locus_tag="NBI57_000724"
     CDS             17042..17725
                     /gene="isdC"
                     /locus_tag="NBI57_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme uptake protein IsdC"
                     /protein_id="HCD1821690.1"
                     /translation="MKNILKVFNTTILALIIIIATFSNSANAADSGTLNYEVYKYNTN
                     DTSIANDYFNKPAKYIKKNGKLYVQITVNHSHWITGMSIEGHKENIISKNTAKDERTS
                     EFEVSKLNGKIDGKIDVYIDEKVNGKPFKYDHHYNITYKFNGPTDVAGANAPGKDDKN
                     SASGSDKGSDGTTTGQSESNSSNKDKVENPQTNAGTPAYIYAIPVASLALLIAITLFV
                     RKKSKGNVE"
     gene            17725..18801
                     /gene="isdD"
                     /locus_tag="NBI57_000725"
     CDS             17725..18801
                     /gene="isdD"
                     /locus_tag="NBI57_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-regulated surface determinant protein IsdD"
                     /protein_id="HCD1821691.1"
                     /translation="MRNVKQIATKSIIAIISLCILTYTTMIGSVLADEIKYPSAKFNQ
                     PEAKDKTELTTSIFDEKIKENKALELLIFNQENKNVTEEQQLVDEKAQLISDMTGKIY
                     LQVKLKGQIDKEQLVFQNDKNEEFPFVIKDEKDDTIVRILIEQHMDKINMHVKTLAEK
                     KNLDNKEMVYSIHFKEKKVQHDDAKEVPSKHQNQENNQDQLKKDIDDKKDSQKSDTKE
                     RRTSLFTEKGLNDIPVQKDKVQQDSNKKIENERPKASGTLKVENSPPTIKKVENNHKE
                     QPKHKDEKSKKEKKKVVEKEKALPAFNRDDDSKNSSQLSSDIKELDEPNHKKQYMLFA
                     AGIVLATILLISAHLYSRKRGNQV"
     gene            18798..19676
                     /gene="isdE"
                     /locus_tag="NBI57_000726"
     CDS             18798..19676
                     /gene="isdE"
                     /locus_tag="NBI57_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006190048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter substrate-binding protein
                     IsdE"
                     /protein_id="HCD1821692.1"
                     /translation="MRIIKYLTILVISVVILTSCQSSSSQESTKSGEFRIVPTTVALT
                     MTLDKLDLPIVGKPTSYKTLPNRYKDVPEIGQPMEPNVEAVKKLKPTHVLSVSTIKDE
                     MQPFYKQLNMKGYFYDFDSLKGMQKSITQLGDQFNRKAQAKELNDHLNSVKQKIENKA
                     AKQKKHPKVLILMGVPGSYLVATDKSYIGDLVKIAGGENVIKVKDRQYISSNTENLLN
                     INPDIILRLPHGMPEEVKKMFQKEFKQNDIWKHFKAVKNNHVYDLEEVPFGITANVDA
                     DKAMTQLYDLFYKDKK"
     gene            19689..20654
                     /gene="isdF"
                     /locus_tag="NBI57_000727"
     CDS             19689..20654
                     /gene="isdF"
                     /locus_tag="NBI57_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000594537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemin ABC transporter permease protein IsdF"
                     /protein_id="HCD1821693.1"
                     /translation="MIKNKKKLLFLCLLVILIATAYISFVTGTIKLSFNDLFTKFTTG
                     SNEAVDSIIDLRLPRILIALMVGAMLAVSGALLQAALQNLLAEANIIGVSSGALIMRA
                     LCMLFIPQLYFYLPLLSFIGGLIPFLIIILLHSKFRFNAVSMILVGVALFVLLNGVLE
                     ILTQNPLMKIPQGLTMKIWSDVYILAVSALLGLILTLLLSPKLNLLNLDDIQARSIGF
                     NIDRYRWLTGLLAVFLASATVAIVGQLAFLGIIVPHVVRKLVGGNYRVLIPFSTVIGA
                     WLLLVADLLGRVIQPPLEIPANAILMIVGGPMLIYLICQSQRNRI"
     gene            20716..>21208
                     /gene="srtB"
                     /locus_tag="NBI57_000728"
     CDS             20716..>21208
                     /gene="srtB"
                     /locus_tag="NBI57_000728"
                     /EC_number="3.4.22.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class B sortase"
                     /protein_id="HCD1821694.1"
                     /translation="MRMKRFLTIVQILLVVIIIIFGYKIVQTYIEDKQERANYEKLQQ
                     KFQMLMSKHQEHVRPQFESLEKINKDIVGWIKLSGTSLNYPVLQGKTNHDYLNLDFER
                     EHRRKGSIFMDFRNELKNLNHNTILYGHHVGDNTMFDVLEDYLKQSFYEKHKIIEFDN
                     KYGK"
BASE COUNT         7240 a         2982 c         3795 g         7191 t
ORIGIN      
        1 tagagttgta gaaatcgaaa atgtttcaca acaaaaacgt gaactacctg ttgttgcata
       61 gtcatttttg aaattattat ttctgaagcg gaggttttaa ttgtctcaag aatgttaaat
      121 tttgaaaatt tgaaacataa ctatagcaaa acagagcgct ttaagataaa attttattat
      181 ctaattagtt agaaaattct aatagtttga ggagtgtatc gctattgaaa caaataaaaa
      241 agttacttgt tgctaaccgt ggagaaattg caattcgtat attcagagcg gcggcagaat
      301 tagacatcag cacagttgca atttattcga atgaagacaa aagttcatta catagatata
      361 aagcagatga atcctattta gttggaagtg atttaggtcc tgctgaaagt tatttaaata
      421 ttgagcgtat cattgatgta gcaaaacaag cgaatgtgga tgcgattcat cctggctatg
      481 gatttttaag tgaaaatgaa caatttgcgc gtcgttgtgc agaagaagga attaaattta
      541 ttggtcctca tttagaacat ttagatatgt ttggagataa agttaaagct cgtacaacgg
      601 ctatcaaggc agatttacca gttattcctg gtacagacgg tccaattaaa tcatatgaat
      661 tagcaaaaga atttgcagaa gaagctggtt tcccgctaat gattaaagcc acaagtggtg
      721 gcggcggtaa aggtatgaga atcgttcgtg aagaaagtga attagaagat gctttccata
      781 gagcaaaatc agaagctgaa aaatcatttg gtaatagtga agtttacata gaaagataca
      841 ttgataatcc aaagcatatt gaagtacaag tcataggtga cgaacatgga aatatcgtac
      901 acttatttga acgtgattgt tcagtacaac gtcgtcatca aaaagttgta gaagttgcac
      961 catcagttgg attatcacca acattacgtc aacgtatttg tgatgctgca attcaattga
     1021 tggaaaatat taaatatgtc aatgcaggta ctgttgaatt tctagtatct ggtgacgaat
     1081 tcttctttat agaagttaac cctcgtgtac aagtagagca tacaattaca gagatggtaa
     1141 caggaattga tattgttaag acacaaattt tagttgcagc aggtgccgat ttatttggtg
     1201 aagagattaa tatgccgcaa caaaaagata ttacaacatt aggctatgcc atccaatgtc
     1261 gtattacaac agaagatccg ttaaatgatt tcatgccgga tactggaaca atcattgctt
     1321 atcgttcaag cggtggcttt ggtgtacgtc ttgatgctgg agatggtttc caaggtgctg
     1381 agatatcacc ttattatgat tcattactcg taaaattatc tacacatgcg atatcattta
     1441 aacaagcaga agaaaaaatg gtacgttcat tacgagaaat gcgtattcgt ggtgttaaaa
     1501 ctaatattcc attcttaatt aatgtaatga agaataaaaa gttcacaagt ggtgattaca
     1561 caactaaatt tattgaagaa acaccagaac ttttcgacat tcagccgtct ctagatagag
     1621 gtactaaaac attagaatat ataggtaatg taacaattaa tggtttccca aatgttgaga
     1681 aacgtccgaa accagactat gaattagcat caattccaac tgtatcttca agtaaaatcg
     1741 cttcatttag tggtacgaaa caattgcttg atgaagtagg tccaaaaggt gtagctgaat
     1801 gggttaaaaa gcaggatgat gtcttactaa cagatacaac ctttagagat gcacaccaat
     1861 cattattagc tacacgagtt agaactaagg atatgattaa tatcgcatcc aaaacagcgg
     1921 acgtatttaa agatggtttc tcactagaaa tgtggggcgg tgctacattt gatgtggcat
     1981 ataatttctt gaaggaaaac ccatgggaac gacttgaacg tctacgtaaa gctatcccaa
     2041 atgtattatt ccaaatgttg ttacgtgctt caaacgcagt tggttataaa aactatcctg
     2101 ataatgttat tcataaattc gtacaagaaa gtgctaaagc aggcatagat gtctttagaa
     2161 ttttcgattc attaaactgg gtagatcaaa tgaaagttgc caatgaagca gtacaagaag
     2221 cgggcaaaat ctctgaaggt actatttgtt atacaggtga cattttaaat cctgagcgat
     2281 caaacattta tactttagag tattatgtca aactagctaa agagttagaa cgtgaaggtt
     2341 tccatatttt agcgattaaa gatatggcag gcttattaaa acctaaagcc gcttacgaat
     2401 tgattggtga gttaaaatca gctgtagatt taccaattca tcttcacact catgatacaa
     2461 gtggtaatgg tttattaaca tacaaacaag caatagatgc tggggtcgat atcattgata
     2521 ctgctgttgc ttcaatgagt ggtttaacaa gtcagccaag cgccaattcg ttatattatg
     2581 cattaaatgg cttcccacgc caccttagaa ctgatattga aggtatggag tcacttagtc
     2641 attattggtc aactgtacgt acttattatt cagactttga aagtgatatc aaatcaccga
     2701 atactgaaat ttatcaacat gaaatgcctg gtggacagta ttcgaattta agtcaacaag
     2761 ctaaaagttt aggtttaggc gaaagatttg atgaagtcaa agatatgtat cgcagagtga
     2821 atttcttatt tggtgatatc gtaaaagtaa ctccatcgtc taaagtagtt ggtgatatgg
     2881 cactttatat ggtacaaaat gatcttgatg aacaatccgt gattacagat ggctataaat
     2941 tagatttccc agaatcagta gtgtcgttct tcaaaggcga aataggacaa cctgtaaatg
     3001 gttttaataa agatttacaa gcggttattt taaaaggcca agaagcacta acagctcgtc
     3061 caggtgaata tctagagcca gttgattttg aaaaagtccg tgagttgctt gaagaagagc
     3121 aacaaggtcc tgttacggag caagatatta ttagttatgt attatatcca aaagtatatg
     3181 aacaatatat tcaaactaga aatcaatacg gaaacttatc gttacttgat acgcctacat
     3241 tcttctttgg aatgcgtaat ggtgaaacag tagaaatcga aatcgataag ggtaaacgat
     3301 taattattaa actagaaacg attagtgaac cagatgaaaa tggtaataga acgatttact
     3361 atgcgatgaa tggtcaagcg agacgtattt acattaaaga tgaaaatgtg catacaaatg
     3421 cgaacgttaa gccaaaagca gataagagta atccaagtca tatcggtgct caaatgccag
     3481 gttcagtaac tgaagtcaag gttagtgttg gtgaaactgt gaaagctaat cagccgttgc
     3541 taattactga agctatgaaa atggaaacaa caattcaagc accatttgac ggtgtgatta
     3601 aacaagtaac tgtaaataat ggtgacacaa tagcgacagg cgatttatta atcgaaattg
     3661 aaaaagcaac tgactaaaat gattaaaata aaacgagatt acacaacaat tgacgtgtaa
     3721 tctcgttttt acatggctta ggtttaattt taaatattga tataaaaaac cgtaatttta
     3781 atgggcgtgc cattaaaatt acggtttgct tttttgttat tgcttgtcac ttcttactga
     3841 tcgtaacatt agcatgataa agtatgttgt cataccaaat aaataagtga taaataatgc
     3901 atggaaaagt gcgattatca ggttaacatt tgtcataata gataatgcac ctgtgataac
     3961 ttgtaatata acaagtataa atgcagctgt ataaccataa tgaacagtac gattatttgg
     4021 ataattttta acagcgtgaa tataagtaat cataataatc gtaaacacta taaaagccat
     4081 gatacgatgc gtgagttgaa cccaatcttg ttctgaatgt ggtacaagat cgtggaatgg
     4141 caatggccaa ccaccatatg ctaaacttgc atccgcatgt ctcactagtg caccagtata
     4201 aacaccacaa taaatgatga ttgccattaa ccatgttaaa cgtcttaatg gctttttgat
     4261 atataattcg tcagcttcat atttttgatc tatagagaaa ataatcaatg ttattaaaaa
     4321 tacagatgag aaactgatta atgatatacc aaagtgcaat gctaaaacgt aatcgttttg
     4381 ttgccaaata acagcggcag ctccgattaa tgcttgcaat aataagaatc caacactaat
     4441 gattgataaa ggtttaattt ctttaatata gcctatatgt ttccatgcag tgataactaa
     4501 ccataagacc attaataaag acaaagctga aacggctcta tgacttaact caataatcgt
     4561 atcaatagga aagaattctg gaatcaacgc accatgacat agtggccaag aagaaccaca
     4621 accatcagct gatccggttt tggtaactaa ggctccacca agttgtacaa atgtcatcat
     4681 taacgttgct acgacaccta accattttaa attctttttg ccaaacaatt tatacacccc
     4741 atcataaaaa aacgcatcac tgagaattag aattaatagc cggatttgag atgatactat
     4801 aaattgacct gattgtagga tatgaaaaga tgggatccaa cgcaggaaat atgatttaat
     4861 tttacaagta ttgtatgtgg acgacataaa aagaacttaa tcgtaatgat ttttttattg
     4921 ggataattat gtaatccacg tttactcaaa tttgaagctt aaatattaat ctcttttaga
     4981 tgcatgattc tacttattaa aattttctaa catatacatt atagcaataa attaaatgtt
     5041 atttgtgtca caaaattgac attttctctt gtcgttaatt tgtcacattt taattttatt
     5101 aaacctagtc tttttatgca atctaagata tcataatctt atatgaacaa atttaaggag
     5161 ggggattatg agcaaagagc atactttgtc acaaaatatt agcagagtta acttcaaaga
     5221 attgcaacag ataattaaaa tgggacttgt tcaaggtaac ttaattccgg cttttgcggg
     5281 cgcatggcta gcagttgtaa tgacaaatca ttccttctta tcatcaatac ctcaaatttt
     5341 attaatgcta tttggatcta ctttaattat gggtggcgca tgtgcgttaa ataattatta
     5401 cgaccaagat attgatcgta ttatgcctag taaacaaaat agaccaactg taaataatag
     5461 aattacagat caaaatttat tactattaag ttttggtatg atgttagttg gagaaatttg
     5521 tttgttttta ttgaatatac catcaggcgt acttggtctt atggggattg taggttatgt
     5581 gtcttattac tcaatatggt ctaaaagaca tacaacatgg aacacagtga ttgggagttt
     5641 tcctggagca gtaccaccac taattggatg ggttgcaatt gaaggacaaa ttagtttaac
     5701 agcgattgcg ctgtttttag ttgtattttg ttggcaacca attcattttt atgccttagc
     5761 tattaaacgt aaagatgaat atgcacttgc aaatattcca atgttaccat cagttaaagg
     5821 ctttaaacgt acacgtgtca gtatgtttat ctggttgatt attttattgc cagtaccttt
     5881 attactaata aatttaggtg tcgtattcgt agtgttagct acattattaa atttaggatg
     5941 gattgcatta ggtttaacaa catttaagaa aaattcagat caaacaaaat gggcaacaca
     6001 aatgtttata tattcactaa attatttagt gatctttttc gtgttagctg taattgtttc
     6061 attacttact ttgatctaga ttaattaagt taggatgaaa aatatgggcg ttccaatttt
     6121 accaacgatt agtacgacat gtattgtcat tagtgcaatt ttaattgcca ttggttggag
     6181 acttatttgg aaaagggaaa taaataaaca caaaaatgtt atgttagctg cagctgtttt
     6241 tgctttaact tttttcttaa tctatgcaag tagaacgatt tttatcggta atacagcatt
     6301 tggcggacca gcatcaatta agaaatatta tacgattttc ttatttttcc acattaattt
     6361 agcaacaatt ggtggtattc taggcctagt tcaaattatt actgcattta aagataaata
     6421 taatgtacac cgcaaattcg ggccattcgc ttcagttata tggttctgta ctgcaattac
     6481 aggtgtagca gtttacttat tattatatgt attatatcca ggtggagaaa cgacatcact
     6541 gattaaagca acatttggtc attaatatgc ttatacaaat gatactaatc tgataaggca
     6601 atttgtttta tcaggttttt tgatgaagaa tagtcggaca gttgatggta ttcatttgat
     6661 accttaactg tccatttttt tatacatatt ttaatcttaa taatgaaatt caacctgaaa
     6721 acataaagat aggttatgat atagagtgta gggaaaatct ttatgacgtt aaaattatgc
     6781 ctgaagatga ttttgtttta tcgaaacgac acagttatgt cagtgttgaa aatttgctaa
     6841 ttaatgtgtt gttttttaca atagtattaa tactttttag gtggttgatt gatgaaaaag
     6901 ttaattataa gagtcgtcgg tgtattattt ttagttggat ttttaatata tcttttttat
     6961 tcaccgagat taaaatttga tgtattagag aacccgaata aaggtaataa agtaaataga
     7021 tctgaacagg tgaataaatc aaataaccat gctgaaaatc caaagcctaa agaaggtgtt
     7081 ggtacatggg taggtaaaga tattaaagtg cttacttcta aatttggaca agcagatcgt
     7141 gtctaccctt ttagagatgg ttacaaaaat tatgtgttta aagacaaaaa cagttattac
     7201 attgtttcaa ctaaacgtga agaaatcgtt tcagtgtatg ctacaggtga gaaagtcaat
     7261 gttagtccgt taaaaatagg acaacattct gcagaaattt ttaatcatac aagtattaat
     7321 ccagaacctt cctttaaagt tgatggtaaa aaatatgaat ttgaactttc agatgaagat
     7381 ttaaaaacac aaacactgat taaatatggc gacatatatg cacaagtgta ttctgatcaa
     7441 caatcgaaaa aggtgctaag tgtacgattt ttaacaaaag aaatgttagc agatattgaa
     7501 ccttatcaat taaattctaa ttctacgtca gaagagcata ataagcgtcc agttgagcaa
     7561 aatccaaatc aattaatttc tctttatgaa gtaacgaatg aaatgagaaa attaaaagga
     7621 ttaaaaccat tgaaaatcaa tagcgattta gcacatattg catctaataa cttatatgaa
     7681 gcgacctcta atggttctga tagtgttgaa tttacagagg acgcattaag agggcaatta
     7741 gataaaaatc acgttactta taaaacaact gctcaaaatg ttggttatgc gtttaatgat
     7801 gtaccaacat taatccatag ttggatgaat tcagatatac atcgatctcg tctattaaat
     7861 tcaaaatacg atgagatggg tggagatgta atgagagatt attattcact aattttctta
     7921 gaaaaataac aatgaggggt atttatgatt aatgaagctt cactagcgat attagatgat
     7981 attgatgaac tagctgatat gatagttgca tcagatattt atgcatcatt tgagcaagct
     8041 aaacaggcgc tcgaaaacaa tgacgaagca catttacttt atcaatcatt tttaaaatca
     8101 aaagagaaat atgatgaagt aatgcgtttc gggaaatatc atcctgatta taagaaagtc
     8161 atgttagaga caagacaacg caaaagagca tatgagatgc ttgacgttgt gatgcattac
     8221 aaagctaggg aaatggcact tcaacattta attgatgaag ttgttacgaa aattgcgtac
     8281 gctgtttcag agcatgtcaa aatagaaaca ggtaatccat tctttcaaac atcacatagt
     8341 ggttgtgcga cgggcggatc ctgtaattgt tcattataaa aaacatcgag tcagaaaaag
     8401 gtggttattg aaaccactaa ctagcatctg actcgatgtt tttatttatt cgggattgtt
     8461 tgtttgaatt gttgtgctaa atctggtcga tctgtcacaa tcgtgtgtgc acctttttgg
     8521 tataaatcat tcatcagatc tatactattt acgccataat agcctggaat gatattcata
     8581 tcatttaacc atttgataaa acgagatgaa gtcaaatcaa tgcctttaaa atgagtaggt
     8641 atttggaacg tttgtgctaa tggttggtag tacctaccac ctaataaatg atattttaaa
     8701 aatgcttctg taacttcctg ttggctagca ccaattgcga cggatccttg tgcaatttta
     8761 ttaaaacgaa cgatttgttc tttataaaaa cttgtcacaa gaacgcggtc aaatgcttga
     8821 ttttctgcaa ttgtatcaaa cataatttgt ggtgcgattg agccttcata ggattcagga
     8881 gcatctttta agtctacgtt tatatacata tcaggatatt gcttcagcaa ctcatcgaag
     8941 gttagtatag ttgtgtgtgc atgaccacga tatggtgtta gtccattgat atctttgaag
     9001 tgataagctg cgtctaattt ctttaattct gctaatgtgt gggcactaac ttttccagag
     9061 ccgttcgtcg ttctatcaac agttgcgtca tgaaaaacga taagctgttg atcttttgtg
     9121 agtctcacat ctgtttcaaa gccatcaacg cctaattgtt tagcatagtc aaatgcaagt
     9181 tgcgtttgct ctggtcttaa agccatacca ccgcgatgcg caaatatata tggtgcattg
     9241 cctttgaaaa aagcagggat ggtttgcttt ttagtaatca ctttattttt attgatcatt
     9301 aatagactac ttaaaaatcc agcaccgact agtaccgcat ttaaaatgtt tctgtttact
     9361 tttttcatag aaaattcctc ctcgtttact tttatagctt aacaaaatca tagttctgac
     9421 tacagcgaaa tgtgtggtta atcatatagt gatgctcttg agaattgaat taatataatg
     9481 atgtttaata taatctaaat acatttatcg agcaattaca taagacataa ttaaggtagc
     9541 ttaatatttt aaaattaata aagacatgat atgattaatg ctgaaaataa atggagtgag
     9601 gtttaaatga atttaatccc aagaactagt attgtagttt atttaaaaca tatgaaacat
     9661 gaacgacaaa tccgaaaata tggacatatc gttcattcaa atagagatcg taaatttgta
     9721 attatgtatg tgaatgagca ggatgttgat caaattgtac ataaactaat gcaacttaaa
     9781 tacgttagac acattgatgg ctcaccatat aaatacttaa agaaaactta cgaaaaagag
     9841 aaacacgaaa tatataatta atattattga agttctggta tccatttttg gagccttaac
     9901 acactaatta aataattata atataacgat aaatcatgaa atgcttgagg gggctgtaca
     9961 tcgataccta aaattggtat gtcgaatttt tcagcacaat cattaatcat ttgaaacttt
    10021 ttatttgaat ctggatatgg atatttaata gcgttaatca tgatataaaa tgcttggaaa
    10081 tgattgccag ttgtagggtt cataatgatt gctacagatg actttttatt ttcagcttgt
    10141 ggtgaatgaa aatagataat tttatcaata cgttgatggt tatattcaat catcgtcatg
    10201 aattcgaaca aatctgttag accttcacca agtgtaataa atgtttgttt cattggttta
    10261 tcattctcct ttttctcaat aaattatatt aaataactaa atgaattgga agtgtgaaca
    10321 acatgcgcgt cattgcaggt aaacataaaa gtaaagcttt agaaagtatg gaaggccgta
    10381 atacgagacc aactatggat aaagttaaag aaggtatctt taatagttta tatgatgtgt
    10441 caggtatagg tttagattta tttgcaggaa gcggggcgct tggaatagaa gcactctctc
    10501 gaggtatgga taaggtaatc tttgttgatc aaaattttaa agctgtaaaa gttattaaat
    10561 caaatcttgc gaatttggat ttagaggcac aatctgaagt ttataaaaat aatgcagata
    10621 gagctttaaa agcattgtca aaacgtgata ttcaatttga tgtcattttc ttagatccac
    10681 cttataataa aggtctcatt gataaagctt taaaactaat ttcagagttt aatttattga
    10741 aagaaaatgg tatcatcgtt tgtgaattta gcaatcatga agaaatagac tatcaaccgt
    10801 ttaatatgat taaacgttac cattatgggt tgacagacac attgttatta gaaaagggag
    10861 aatagcatgg aacatacaat agcggtcatt ccgggtagtt ttgaccccat tacttatggt
    10921 catttagaca ttattgagag aagtacagat agatttgatg aaattcatgt ctgtgttctt
    10981 aaaaatagta aaaaagaagg tacgtttagt ttagaagagc gtatggattt aattgaacaa
    11041 tctgttaaac atttacctaa tgtcaaggtt catcaattta gtggtttact agtcgattat
    11101 tgtgaacaag taggagctaa aacaatcata cgtggtttaa gagcagtcag tgattttgaa
    11161 tatgaattac gcttaacttc aatgaataaa aagttgaaca atgaaattga aacgttatat
    11221 atgatgtcta gtactaatta ttcatttata agttcaagta ttgttaaaga agttgcagct
    11281 tatcgagcag atatttctga attcgttcca ccttatgttg aaaaggcatt gaagaagaaa
    11341 tttaagtaat aaaaataaca gtattttagg tttatcatgg tttacaatcc taaaatactg
    11401 ttttcatttg ttaacgatat tgctgtatga caggcgtgtt gaaatctgtt tgttgttgcc
    11461 cgcttattgc attgtatatg tgtgttgctt tgatttcatt tgtgaagtaa tgtgcattgc
    11521 ttttgttaat attggttata tattgtcttt ctgggaacgc tgtttttaaa tgctttaaat
    11581 attgtctgcc acggtcgttc atcgctaata ctttaactgc gtgaatgtta ctcgtaacat
    11641 ctgtaggttt aatgtttaat aatacattca ttaacagtct ttggatatgc gtatatgtat
    11701 aacgctttgt ttttagtaat tttacaaaat gatgaaaatc agttgcttca taaatgttag
    11761 atttcaaacg attttcaaaa ccttcagtaa cagtataaat attttttaat gaatctgtag
    11821 tcatagctat gatttgatat ttcaaatatg gaaatatttg atttaatgtt atatgaggtg
    11881 ttacgtacaa gtgttgaata tctttaggta ccacatgatg ccaatgatca tcttgactaa
    11941 tgattgatgt tctaatagat gtaccacttg caaactgatg gtgttgaatt aatgaatcat
    12001 gatgttgagc attttctcgt ttgatagaaa ttgcattgat gtttttagca tttttagcaa
    12061 ttgctttcag gtaactaata ccaagtatgt tgttaggact tgctagtgct tcatgatgct
    12121 ctaataattc gctaatgata cgagggtagc ttttaccttc ttttactttt tgtgaaaagg
    12181 attcagattg ttcaatttca ttaatgctgt gtgctaattg ctttaatgtt ttgatatcat
    12241 tattttcact accaaatgca attgtatcga cactcatata atctgcgact ttaactgcta
    12301 gttcggcaaa atgatcgcca gatgataaac tggcagttgc tggtagttcg ataactaaat
    12361 cagctgttga taatgccatt tttgcacgag taaacttatt atagattgct ggttcgccac
    12421 gcatgacaaa gttaccactc attattgcaa tagtaacgtc agcatttgta agttttttag
    12481 attgattaat atgatattga tgcccattat gaaagggatt atattctgtg attaagccaa
    12541 cgcttttcat ttgcaattcg tcctttcggt attcattatt aatattgtaa cagaacatga
    12601 tatgttaaga aaaaatcttg acaacttgtt cttagaaagt taaaataaat tttgtgcttg
    12661 ttagaggtga agccatatga aatggtcaat tacgcaatta aggaaatatc aaggtaagcc
    12721 atttgaattt gatcaaacgg tgagttttga caatttaaaa gaatcattag atttaattga
    12781 tttatctcca attacaatcc aaggtcagtt aaccattaag tcaacagaag tcgttgcgga
    12841 tattcacatt actggaacgt atacaatgcc ttgtgcacgt actcttgtac cagtaaaagt
    12901 cccactagat gtaactacta cagaagtatt tgatttagaa gggtacaatc agtataacga
    12961 tgatcaagat gatgtagatg aacactatca cattattaaa gatggtatgg ttaatcttca
    13021 ggatattgtc gaggatatag ttattattga gaaaccaatg agagcttatt cagagcaaag
    13081 tgaccaaatg ttgacagtag gtaatggttg ggaagtaatc gatgaggatc aattagatga
    13141 gcttgctaaa cagcaagaac aagatgattc agaatcacga caagttgatc caaggcttca
    13201 aaaattacaa caattatatg ataaagagca ataagtagtt taattaatga tataatgttg
    13261 ttaattaaac taacatttag tatttttcgt ttataatcta aggaggatat atcatggcag
    13321 taccaaaaag aagaacttct aaaactagaa aaaacaaacg tcgtacgcat ttcaaaattt
    13381 cagtaccagg tatgactgaa tgcccaaact gtggcgaata caaattatca caccgtgtat
    13441 gtaaaaactg tggttcttac aatggcgaag aagtagcagc taaataattt tagttactca
    13501 atataaaaag tcccgcttaa aatgattgtt ttaagtggga ctttttatat tgcgaaaaat
    13561 aattggcgaa cgaggtaact ggatacctca tccgccaatt aaaatttgtt aatttaataa
    13621 ttaaatataa agacgattta ttagttttta cgttttctag gtaatacgaa tgcaacgatg
    13681 ctacttaaag ctagtaatgc cattaatggt aatgtcatat ctttatttga ttcttcacca
    13741 gtttgtggta atgattttgc tttattttct tgtgtatttt tattgttttg gctttgagtg
    13801 tgtccatcat ttgtgttttt aatgtttgct ttttgtaatg gagcactatc ttttgcttcg
    13861 ctagaacctg ctgaagtttg aacaacatct tttgttgttt ttgatgaagc agttgttggt
    13921 tttgcaacat tttgagtcgt agatactacc ttagttggag ttgtactact tgattctact
    13981 tcacctttag ttggttttgt agcaggcgtt ttgtctttac ctgactcact agatgcgtca
    14041 ttttcttttt caacacttgg taattgttta ttgtcatctt tttggctgtc ttgtttttgt
    14101 gattcttttt caacaggtga tggtgttggt ttgctaggcg tagctggagt agcttccttc
    14161 ttagctgagt tatcttgttg ttcttttttg ttagatttat cggtattggc ttttgtaaat
    14221 gcttctttat caacgattct gacatggtat tgtccatcat aatcaatcgt ttttacgtga
    14281 actttaacga tagcatcata tagagtttta ccttcaacat atgggaaaat aattgttcta
    14341 gtattatttt tagcatcttt gcttatagtt ctaacacgtt gaccttcaac catgaaatct
    14401 ttccagtaat cgtcattagt agtttccatg accatatatt ttttgccgtt aagcatacct
    14461 gttttaatag ggtgtttaac aaaagtatcc atcatagatt cgttattctc aacactttca
    14521 taaacaacat attttgtatc ttgtaaatca gtcatttttt catttgttgg ttgtacattt
    14581 tggaattcag taatagctga tttcacttgc tcatctaaag ctttctttgt atcctctaat
    14641 ttcttcttgt actcagcctt taatttttca ggaagtttat cttgaatttt atttaattca
    14701 taaacttgtc tttctagtgt tttcgctttt ttatatggcg ctaataattt ttcagcttta
    14761 taatcttctt cagttttgaa tttatctgca ctgttataaa ttggttgtgc gaattccatt
    14821 aatgtgtaat cgtatttttc ttctttgtta ttgaagtgag ttgaacttac aattttaacg
    14881 gcttttgttc catttgaaac agagaagcga atgtaagcgt aatctttaac agtatcgtat
    14941 gatactaatt taattggcaa ctttttgtca ccttcataaa cttcaaattt tctccaaaat
    15001 tgacctgatt gtaatcctaa ttcaatttct ggttttgaat cagtgaaaat aactctagca
    15061 ggtttaacag agctggcata atgataaaat tgttgctcac cattttcttt tttcatttca
    15121 aaatcaattg gacgagagtt tggtgcgcta tgatctttat cttttattgc agggttttta
    15181 atcgcttctc taagttcctg attcaaaata ggatatgtat tgttagtggc ttttgctgct
    15241 ggtttaactg cttttgtttc cttaggggct ttaacttctt taacttcttt agcttctttt
    15301 gtttcagaag taggggcctc aacttcttta ttagatactg agacagcatt agctactggt
    15361 ttagtttctg gagctttttc agatgttgtt gttggacttg caactgcttc agtttttggt
    15421 tgtgcttctg tatttgtacc acctgtttct tcagctgctg cttgtgcttc gccatttgac
    15481 attaataata aaagtgtact aatcgctaca gatgcaacgc ctagtgatga ctttctaatt
    15541 gaataaaatg atttaaattc tttttgctgt ttgttcatgt tgtagaaaca actcctaatt
    15601 gtatattatc aactgataat cattatcaat tgattacttg tattgtacat tgaaatggta
    15661 gttaattgca acattatttt ataaaaaaat aacctatgtt atagattatg aaatatctat
    15721 gacataggtt aaataaaatt taaaataaaa aatatattaa gttatgagtg tgacgatgat
    15781 ttatttagat tcttttcttt tgaaaagtaa taaagataat gaacctaaaa gggcaagtgt
    15841 tgcgaaggca actgtgctaa taaagttatc aactgaagtt aaaccagttt ttggtaattc
    15901 tttagcttta gatgcttgtt taggcgtttc gttatgtttt gtaactttgt tagtttgttg
    15961 tgatttatta tcacttacag cttgattgtt gctttcagat ttcgctgttg caacatcttt
    16021 aacaggtgtt tgaactttat tttgttcttg agcagtttgt gctgttttaa ctgtatgagc
    16081 agtttgtgtt ttagtttgat cttttgttgt gtcagtactt acaactttag tagagtgatt
    16141 gtcttcaact ttgcttgttg tagttacagt aggtttaact ttttcaactt taggttgaac
    16201 tggtttagtt tgctcagtag gtgttttagg ttgagctggt tttggttgaa ccggtttaac
    16261 attgtttggt tttgctgcgt cagctaatgt aggaattgct ttttcaaatt ccaaatgcgt
    16321 agtatatcta tgattgtaat taatttgtgg cacgacaata tgtactttag tagttaagct
    16381 cttatatcca ggttcaactg caacattgat tgttctagta tccgcttttt tattatcgtt
    16441 aacaacagtt gttgctaatt cttgattgtt tgcattgtaa aatttgtatt ctttccagaa
    16501 tgatgcattg tttaacacgg tttggaaata atatttatta ttttgtttaa ttactttacc
    16561 agggtgttgc atatagtcat ccatgtgtga cttctctgaa gagccatctt tttgcacttg
    16621 gaaattaatt ggttgtgatg ttgcttgaga aacttgtgtg ctttgattat tagttgcgtt
    16681 cgtagcttct gttgccgcat tgacttgttg gctgtctgcg cctatgtata caagggaacc
    16741 taaaatgata gatgctgtac ccattgtaat ctttttcata gctgatgaac gttgttctga
    16801 ttgatactta ctgtttaaat aatgttttgt catgttgttt tcctcctaag gatacaaatt
    16861 atgtgttgtt atatgagatg attaaccatt gcaattgata atggttatca attatactag
    16921 cacatctatt tccgtataac aataataaat tgattataaa ataaatattg acaatgataa
    16981 tcattattat ttatgatttt actaaagact taaaagcaat cataaaaagg aggattatgt
    17041 tttgaaaaat attttaaaag tttttaatac aacgatttta gcgttaatta tcatcatcgc
    17101 gacattcagt aattctgcaa atgccgcaga tagcggtact ttgaattatg aggtttacaa
    17161 atacaatacc aatgacacgt caattgctaa tgactatttt aataaaccgg caaagtacat
    17221 taagaaaaat ggtaaattgt atgttcaaat aactgtcaac cacagtcatt ggattactgg
    17281 aatgagtatc gaaggacata aagaaaatat tattagtaaa aacactgcca aagatgaacg
    17341 cacttctgaa tttgaagtaa gtaagttgaa cggtaaaata gatggaaaaa ttgacgttta
    17401 tatcgatgaa aaagtaaatg gaaagccatt caaatatgac catcattaca acattacata
    17461 taaatttaat ggaccaactg atgtagcagg tgctaatgca ccaggtaaag atgataaaaa
    17521 ttctgcttca ggtagtgaca aaggatctga tggaacgact actggtcaaa gtgaatctaa
    17581 tagttcgaat aaagacaaag tagaaaatcc acaaacaaat gctggtacac ctgcatatat
    17641 atatgcaata ccagttgcat ccttagcatt attaatcgca atcacattgt ttgttagaaa
    17701 aaaatctaaa ggcaatgtgg aataatgaga aatgttaaac aaattgctac aaaatctatt
    17761 atagctatta ttagcttatg tatacttaca tatacaacaa tgattggtag cgtgttggct
    17821 gatgagataa aatatccatc agccaaattt aatcaacctg aagcaaaaga taaaacagaa
    17881 ttaactacat caatttttga tgaaaagata aaagagaata aagcgttaga gttactaatt
    17941 tttaatcaag aaaataaaaa tgtaactgag gaacaacaac tagttgacga aaaggcgcaa
    18001 ttgatttcag atatgactgg taaaatttac ttgcaagtaa agctaaaagg tcaaatagat
    18061 aaagaacaac ttgtttttca aaatgacaaa aatgaagaat ttccttttgt tataaaagat
    18121 gaaaaggatg acacaatagt aagaatttta attgaacagc atatggataa aatcaatatg
    18181 catgttaaaa cgttggctga aaagaaaaat ctagataaca aagaaatggt gtattctatt
    18241 cattttaaag agaaaaaagt acaacatgat gatgcaaaag aagtgccttc aaaacatcaa
    18301 aatcaagaaa ataatcaaga tcagcttaaa aaagatattg atgacaaaaa agatagtcaa
    18361 aaatcagata ctaaggaaag acgtactagc ctttttactg aaaaaggatt aaatgatatt
    18421 cctgtacaaa aagataaagt gcaacaagac agtaataaaa agattgaaaa tgagcgacct
    18481 aaagcatcag gtacattaaa agttgaaaat agccctccaa caataaaaaa ggttgaaaat
    18541 aatcacaaag agcaaccgaa acataaagat gaaaaatcaa aaaaggaaaa gaaaaaagta
    18601 gttgaaaaag aaaaagcgtt accagctttt aatagagatg atgatagcaa gaatagtagt
    18661 caattatcta gtgatattaa agaacttgat gaaccaaatc ataaaaagca atatatgtta
    18721 tttgcagctg gcattgtgtt agcaactatt ttacttattt cggcacattt atacagcaga
    18781 aagagaggta accaagtttg agaatcataa agtatttaac cattttagtg ataagcgtcg
    18841 ttatcttaac cagctgtcaa tcttccagtt ctcaagaatc aactaaatcc ggcgaattca
    18901 gaatcgtacc aacaactgtt gcattgacaa tgacattgga caaattggat ttaccaattg
    18961 tcggcaaacc cacgtcatat aagacattgc ctaatcgtta taaagatgta ccggaaattg
    19021 gtcaaccaat ggagccgaat gttgaagctg ttaaaaagtt aaaaccaaca catgttttga
    19081 gtgtgtcaac gattaaagat gaaatgcaac cattttacaa acaattaaat atgaaaggct
    19141 acttttatga ttttgatagt ttaaaaggga tgcaaaagtc gattacacaa ttaggtgatc
    19201 aatttaatcg taaagcacaa gcaaaagaat taaatgacca tttaaattct gtaaagcaaa
    19261 aaattgaaaa taaagcagct aaacaaaaga aacatcccaa agtattaata ttaatgggtg
    19321 taccgggtag ctatttagta gcaactgata aatcatatat tggtgattta gttaaaatag
    19381 caggtggaga aaatgttatt aaagtgaaag atcgtcaata tatttcgtct aatactgaaa
    19441 atttgttgaa tatcaatcca gatattattt tacgattacc acacggaatg cctgaagaag
    19501 ttaagaaaat gtttcaaaaa gaatttaaac aaaatgatat ttggaaacat tttaaagctg
    19561 tgaaaaataa tcatgtttat gacttagagg aagtgccatt cggtattaca gcaaatgttg
    19621 atgctgataa ggcaatgact caattatatg atttatttta taaggataaa aaatagtgag
    19681 ttgatatgat gataaaaaat aaaaagaaac tactattttt atgtttgtta gtcattttaa
    19741 tcgcaactgc ttatatttcg tttgtaaccg gtacaattaa attgtcattt aatgacctat
    19801 ttacaaaatt tacaactggt agcaatgaag cagtggattc aatcattgat ttgcgattgc
    19861 cacgtatatt aattgcattg atggttggcg caatgttagc agtttctgga gcattattac
    19921 aagcagcact acaaaatctt ttggcagagg cgaatatcat tggcgtttcc tcaggtgcac
    19981 ttataatgag agcactttgt atgttgttta ttccacaatt gtacttttac ttaccattat
    20041 taagttttat tggaggttta ataccatttt taataattat attgttgcat tctaaattta
    20101 gattcaatgc tgtaagtatg atattagtag gtgttgcgtt attcgtatta ttaaatggtg
    20161 ttttagaaat tttaactcaa aaccctttaa tgaaaattcc tcaaggctta acaatgaaaa
    20221 tatggagcga cgtatacata ttagcagtat cagcattatt gggattaata ttaacattac
    20281 tattgtcccc taaattgaat ttactaaatt tagacgacat acaagcgcga agtatcggtt
    20341 ttaatattga tcgttacaga tggttaacag gtttattagc agtattttta gcaagtgcaa
    20401 ctgttgcgat tgttggacaa ctagcctttt taggtattat tgtgccacat gtggttagaa
    20461 agctagttgg gggcaattac agagtactta ttccgttttc tacagttatt ggtgcatggc
    20521 tattgttagt ggctgattta ttaggacgag tgatacagcc tcctttagaa attccagcca
    20581 atgctatttt aatgattgtc ggtggtccaa tgctaattta cttaatttgt caaagtcaac
    20641 gaaatcgaat ctaaaacggt acaacaactg gtttagtgat gactgaagaa acgtcgttaa
    20701 taaaagagga tttttatgag aatgaagcga tttttaacta ttgtacaaat tttattggtt
    20761 gtaattatta tcatttttgg ttacaaaatt gttcaaacat atattgaaga caagcaagaa
    20821 cgcgcaaatt atgagaaatt acaacaaaaa tttcaaatgc tgatgagcaa acatcaagaa
    20881 catgtgagac cacaatttga atcacttgaa aaaataaata aagacattgt tggatggata
    20941 aaattatcag gaacatcatt aaattatcca gtactacaag gtaagacaaa tcacgattat
    21001 ttaaatttag attttgagcg agaacatcga cgtaaaggta gtatttttat ggattttaga
    21061 aatgaattga agaatttaaa tcataatact attttatacg ggcaccatgt cggtgataat
    21121 acgatgtttg atgtgttaga agattattta aagcaatcgt tttatgaaaa acacaagata
    21181 attgaatttg acaataaata tggtaaat
//
LOCUS       DAHHSC010000025        20213 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.025, whole genome shotgun sequence.
ACCESSION   DAHHSC010000025 DAHHSC010000000
VERSION     DAHHSC010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 20213)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20213)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20213
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.025"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            416..907
                     /gene="lspA"
                     /locus_tag="NBI57_000729"
     CDS             416..907
                     /gene="lspA"
                     /locus_tag="NBI57_000729"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HCD1821695.1"
                     /translation="MHKKYFIGTSILIAVFVVIFDQVTKYIIATTMKIGDSFEVIPHF
                     LNITSHRNNGAAWGILSGKMTFFFIITIIILIALVYFFIKDAQYNLFMQVAISLLFAG
                     ALGNFIDRILTGEVVDFIDTNIFGYDFPIFNIADSSLTIGVILIIIALLKDTSNKKEK
                     EVK"
     gene            907..1824
                     /locus_tag="NBI57_000730"
     CDS             907..1824
                     /locus_tag="NBI57_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HCD1821696.1"
                     /translation="METYEFNITDKEQTGMRVDKLLPELNNDWSRNQIQDWIKAGLVV
                     ANDKVVKSNYKVKLNDHIVVTEKEVVEADILPENLNLDIYYEDDDVAVVYKPKGMVVH
                     PSPGHYTNTLVNGLMYQIKNLSGINGEIRPGIVHRIDMDTSGLLMVAKNDIAHRGLVE
                     QLMDKSVKRKYIALVHGNIPHDYGTIDAPIGRNKNDRQSMAVVDDGKEAVTHFNVLEH
                     FKDYTLVECQLETGRTHQIRVHMKYIGFPLVGDPKYGPKKTLDIGGQALHAGLIGFEH
                     PVTGEYIERHAELPQDFEDLLDTIRKRDA"
     gene            2224..2751
                     /gene="pyrR"
                     /locus_tag="NBI57_000731"
     CDS             2224..2751
                     /gene="pyrR"
                     /locus_tag="NBI57_000731"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HCD1821697.1"
                     /translation="MSERIIMDDAAIQRTVTRIAHEILEYNKGTDNLILLGIKTRGEY
                     LANRIQDKIHQIEQQRIPTGTIDITYFRDDIEHMSSLTTKDAIDIDTDITDKVVIIID
                     DVLYTGRTVRASLDAILLNARPIKIGLAALVDRGHRELPIRADFVGKNIPTSKEETVS
                     VYLEEMDQRNAVIIK"
     gene            2969..4276
                     /locus_tag="NBI57_000732"
     CDS             2969..4276
                     /locus_tag="NBI57_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HCD1821698.1"
                     /translation="MQNDEMFERTVKPVLDVNEKPQPAQWAFLSLQHLFAMFGATVLV
                     PLLTGLPISAALLASGIGTLLYILITKAQIPAYLGSSFAFITPIITGLSTHSLGDMLV
                     ALFMSGVMYVIIGILIKLSGTAWLMKLLPPVVVGPVIMVIGLSLAPTAVNMAMYENPG
                     DMKGYNISFLIVAMITLLVTIVVQGFFKGFLSLIPVLVGIIVGYVVAIFMGIVKFDAI
                     MSAKWIDFPHIYLPFKDYVPSFHLGLVLVMIPIVFVTVSEHIGHQMVLNKIVGRNFFE
                     KPGLDKSIIGDGVSTMFASIIGGPPSTTYGENIGVLAITRIYSIYVIGGAAVIAIVLA
                     FIGKFTALISSIPTPVMGGVSILLFGIIAASGLRMLVESKVDFANNRNLVIASVILVV
                     GIGNLVFNLKEIGINLQIEGMALAALSGIILNLILPKEKKQNN"
     gene            4304..5185
                     /locus_tag="NBI57_000733"
     CDS             4304..5185
                     /locus_tag="NBI57_000733"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HCD1821699.1"
                     /translation="MNHLLSMEHLSTDQIYKLIQKASQFKSGERQLPNFEGKYVANLF
                     FENSTRTKCSFEMAELKLGLKTISFETSTSSVSKGESLYDTCKTLESIGCDLLVIRHP
                     FNNYYEKLANINIPIANAGDGSGQHPTQSLLDLMTIYEEYGYFEGLNVLICGDIKNSR
                     VARSNYHSLKALGANVMFNSPNAWIDDSLEAPYVNIDDVIETVDIVMLLRIQHERHGL
                     AEETRFAADDYHQKHGLNEVRYNKLQEHAIVMHPAPVNRGVEIQSDLVEASKSRIFKQ
                     MENGVYLRMAVIDELLK"
     gene            5203..6477
                     /locus_tag="NBI57_000734"
     CDS             5203..6477
                     /locus_tag="NBI57_000734"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HCD1821700.1"
                     /translation="MKLIKNGKVLQNGELQQADILIDGKVIKQIAPAIEPSNGVDIID
                     AKGHFVSPGFVDVHVHLREPGGEYKETIETGTKAAARGGFTTVCPMPNTRPVPDSVEH
                     FEALQKLIDDNAQVRVLPYASITTRQLGKELVDFPALVKEGAFAFTDDGVGVQTASMM
                     YEGMIEAAKVNKAIVAHCEDNSLIYGGAMHEGKRSKELGIPGIPNICESVQIARDVLL
                     AEAAGCHYHVCHVSTKESVRVIRDAKRAGIHVTAEVTPHHLLLTEDDIPGNNAIYKMN
                     PPLRSTEDREALLEGLLDGTIDCIATDHAPHARDEKAQPMEKAPFGIVGSETAFPLLY
                     THFVKNGDWTLQQLVDYLTIKPCETFNLEYGTLKENGYADLTIIDLDSEQEIKGEDFL
                     SKADNTPFIGYKVYGNPILTMVEGEVKFEGDK"
     gene            6479..7579
                     /gene="carA"
                     /locus_tag="NBI57_000735"
     CDS             6479..7579
                     /gene="carA"
                     /locus_tag="NBI57_000735"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HCD1821701.1"
                     /translation="MQSKRYLVLEDGSFYEGYRLGSDNLTVGEIVFNTAMTGYQETIS
                     DPSYKGQIITFTYPLIGNYGINRDDFESLVPTLNGIVVKEASAHPSNFRQQKTLHDVL
                     ELHQIPGIAGVDTRSITRKIRQHGVLKAGFTDRKEDIDQLVKHLQQVELPKNEVEIVS
                     TKTPYVSTGKDLSVVLVDFGKKQNIVRELNVRGCNVTVVPYTTTAEEILAMAPDGVML
                     SNGPGNPEVVECAIPMIQGILGKIPFFGICLGHQLFALSQGASSFKMKFGHRGANHPV
                     KNLETGKVDITSQNHGYAIDIDSLKSTDLEVTHLALNDGTVEGLKHKTLPAFSVQYHP
                     EANPGPSDSNYLFDDFVAMMTNFKEKERHINA"
     gene            7572..10745
                     /gene="carB"
                     /locus_tag="NBI57_000736"
     CDS             7572..10745
                     /gene="carB"
                     /locus_tag="NBI57_000736"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HCD1821702.1"
                     /translation="MPKRNDIKTILVIGSGPIIIGQAAEFDYAGTQACLALKEEGYRV
                     ILVNSNPATIMTDKEIADKVYIEPLTHDFIARIIRKEQPDALLPTLGGQTGLNMAIQL
                     HESGVLQDNNVQLLGTELTSIQQAEDREMFRTLMNDLNVPVPESDIVNTVEQAFKFKE
                     QVGYPLIVRPAFTMGGTGGGICHNDEELHEIVSNGLHYSPATQCLLEKSIAGFKEIEY
                     EVMRDKNDNAIVVCNMENIDPVGIHTGDSIVVAPSQTLSDVEYQMLRDVSLKVIRALG
                     IEGGCNVQLALDPHSFDYYIIEVNPRVSRSSALASKATGYPIAKLAAKIAVGLTLDEM
                     LNPITGTSYAAFEPTLDYVISKIPRFPFDKFEKGERELGTQMKATGEVMAIGRTYEES
                     LLKAIRSLEYGVHHLGLPNGESFDLDYIKERISHQDDERLFFIGEAIRRGTTLEEIHN
                     MTQIDYFFLHKFQNIIDIEHQLKEHQGDLEYLKYAKDYGFSDKTIAHRFNMTEEEVYQ
                     LRMENDIKPVYKMVDTCAAEFESSTPYYYGTYETENESIVTDKEKILVLGSGPIRIGQ
                     GVEFDYATVHAVWAIQKAGYEAIIVNNNPETVSTDFSISDKLYFEPLTEEDVMNIINL
                     EKPKGVVVQFGGQTAINLADKLAKHGVKILGTSLENLNRAEDRKEFEALLRKINVPQP
                     QGKSATSPEEALANAAEIGYPVVVRPSYVLGGRAMEIVDNDKELENYMTQAVKASPEH
                     PVLVDRYLTGKEIEVDAICDGETVIIPGIMEHIERAGVHSGDSIAVYPPQTLTEDELA
                     TLEDYTIKLAKGLNIIGLINIQFVIAHDGVYVLEVNPRSSRTVPFLSKITDIPMAQLA
                     MRAIIGEKLTDMGYQEGVQPYAEGVFVKAPVFSFNKLKNVDITLGPEMKSTGEVMGKD
                     TTLEKALFKGLTGSGVEVKDHGTVLMTVSDKDKEEVVKLAQRLNEVGYKILATSGTAN
                     KLAEYDIPAEVVGKIGGENDLLTRIQNGDVQIVINTMTKGKEVERDGFQIRRTTVENG
                     IPCLTSLDTANALTNVIESMTFTMRQM"
     gene            10855..11547
                     /gene="pyrF"
                     /locus_tag="NBI57_000737"
     CDS             10855..11547
                     /gene="pyrF"
                     /locus_tag="NBI57_000737"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HCD1821703.1"
                     /translation="MKDLPIIALDFESKEKVNQFLDLFDESLFVKVGMELFYQEGPQL
                     INEIKERGHDVFLDLKLHDIPNTVGKAMEGLAKLNVDLVNVHAAGGVKMMSEAIKGLR
                     KHNQHTKIIAVTQLTSTTEDMLRHEQNIQTSIEEAVLNYAKLANAAGLDGVVCSPLES
                     RMLTEKLGTSFLKVTPGIRPKGASQDDQHRITTPEEARQLGSTHIVVGRPITQSDNPV
                     ESYHKIKESWLV"
     gene            11547..12158
                     /locus_tag="NBI57_000738"
     CDS             11547..12158
                     /locus_tag="NBI57_000738"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HCD1821704.1"
                     /translation="MAKEIAKSLLDIEAVTLSPNDLYTWSSGIKSPIYCDNRVTLGYP
                     LVRGAIRDGLINLIKEHFPEVEVISGTATAGIPHAAFIAEKLKLPMNYVRSSNKSHGK
                     QNQIEGAKSEGKKVVVIEDLISTGGSSVTAVEALKQAGAEVLGVVAIFTYGLKKADDT
                     FSNIQLPFYTLSDYNELIEVAENEGKISSEDIQTLVEWRDNLA"
     gene            12188..12400
                     /locus_tag="NBI57_000739"
     CDS             12188..12400
                     /locus_tag="NBI57_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1821705.1"
                     /translation="MNDKTSNDLYGKIKHCNEFINHSNDSNLSSSHDVDESSTKQKHI
                     KNKTTIDHNDDLFKHVKDILRKQGQI"
     gene            12837..13238
                     /locus_tag="NBI57_000740"
     CDS             12837..13238
                     /locus_tag="NBI57_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCD1821706.1"
                     /translation="MDIPKITTFLMFNNKAEEAVKLYTSLFEDSEIITMAKYGENGPG
                     DHGTVQHSIFTLNGQVFMAIDANSGTELPMNPAISLFVTVKDTIEMERLFNGLKDEGA
                     ILMPKTNMPPYREFAWVQDKFGVSFQLALPE"
     gene            complement(13501..15198)
                     /locus_tag="NBI57_000741"
     CDS             complement(13501..15198)
                     /locus_tag="NBI57_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001575279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NFACT family protein"
                     /protein_id="HCD1821707.1"
                     /translation="MAYDGLFTKKMVESLQFLTTGRVHKINQPDNDTILMVVRQNRQN
                     HQLLLSIHPNFSRLQLTTKKYDNPFNPPMFARVFRKHLEGGIIESIKQIGNDRRIEID
                     IKSKDEIGDTIYRTVILEIMGKHSNLILVDENRKIIEGFKHLTPNTNHYRTVMPGFNY
                     EAPPTQHKINPYDITGAEVLKYIDFNAGNIAKQLLNQFEGFSPLITNEIVSRRQFMTS
                     STLPEAFDEVMAETKLPPTPIFHKNHETGKEDFYFIKLNQFNDDTVTYDSLNDLLDRF
                     YDARGERERVKQRANDLVRFVQQQLHKYQNKLAKLIEEYEQSKNKDTEQLYGELITAN
                     IYRIKQGDKEVTALNYYTNEEVVIPLNPTKSPSANAQYYYKQYNRMKTRERELQHQIQ
                     LTKDNIDYFSTIEQQLHHISVHDIDEIRDELAEQGFMKQRKNQTKKKKAQIQLQHYVS
                     TDGDDIYVGKNNKQNDYLTNKKAKKTHTWLHTKDIPGSHVVIFNDAPSDTTIKEAAML
                     AGYFSKAGNSGQIPVDYTLIKNVHKPSGAKPGFVTYDNQKTLYATPDYELIQKMKQL"
     gene            15473..16096
                     /gene="gmk"
                     /locus_tag="NBI57_000742"
     CDS             15473..16096
                     /gene="gmk"
                     /locus_tag="NBI57_000742"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HCD1821708.1"
                     /translation="MDNEKGLLIVLSGPSGVGKGTVRKRIFEDPSTSYKYSISMTTRQ
                     MREGEVDGVDYFFKTRDAFEALIKDDQFIEYAEYVGNYYGTPVQYVKDTMDEGHDVFL
                     EIEVEGAKQVRKKFPDALFIFLAPPSLDHLRERLVGRGTESNEKIQSRINEARKEVEM
                     MNLYDYVVVNDEVELAKNRIQCIVEAEHLKRERVEAKYRKMILEAKK"
     gene            16096..16314
                     /locus_tag="NBI57_000743"
     CDS             16096..16314
                     /locus_tag="NBI57_000743"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HCD1821709.1"
                     /translation="MLNPPLNQLTSQIKSKYLIATTAAKRAREIDEQPETELLSEYHS
                     FKPVGRALEEIADGKIRPVISSDYYGKE"
     gene            16530..17729
                     /gene="coaBC"
                     /locus_tag="NBI57_000744"
     CDS             16530..17729
                     /gene="coaBC"
                     /locus_tag="NBI57_000744"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HCD1821710.1"
                     /translation="MKKILLAVTGGIAAYKAIDLTSKLTQSGYEVRVMLTNHAQKFVT
                     PLAFQAISRNAVYTDTFIEENPSEIQHIALGDWADAIIVAPATANTIAKLSVGIADDL
                     VTSTLLATETPKFIAPAMNVHMYENKRTQQNINILKEDGYHFIEPGSGFLACGYVAKG
                     RMEEPLQIVSVIDAHFQNSNRLANSSFQDKRALVTAGPTIEVIDPVRFVSNRSSGKMG
                     YAIAEALRNRGAIVTLVAGPTTLEDPKDIEVIHVQSAEEMFEQVTSRFDEQDIVVKAA
                     AVSDYTPVDVLEHKMKKQDGDLSVSFKRTKDILKYLGEHKTSQYLIGFAAETEDIENY
                     AQQKLRKKNADVIISNNVGDMSIGFSSDDNELTMHFKNNEKVNIKKGKKVVLAAQILD
                     ELETRWQ"
     gene            17729..20137
                     /gene="priA"
                     /locus_tag="NBI57_000745"
     CDS             17729..20137
                     /gene="priA"
                     /locus_tag="NBI57_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HCD1821711.1"
                     /translation="MIAKVIVDVASKSVDYKFDYIIPEQLESVIQPGVRVIVPFGPRT
                     IQGYVMEVTAEPDAQLDVSKLKKIIEVKDIQPELTSELIALSEWMGSTHVIKRISMLE
                     VMLPSAIKAKYKKAFKMKDDIELPSALLQKFDKHGYYYYKDAQKNNDIQLLMKLLKDD
                     IVEEKTILTQNITKKTKRAVRVIEGYHPDEVLAKLEKVIKQYDLYAYLSEEQHKTIFL
                     TDIEDMGFSKSSLDGLIKKGYVEKYDAVVERDPFKDRVFEQESKQQLTEDQYKAYEAI
                     KAKIVSQEQETFLLHGVTGSGKTEVYLQTIEDVLSQGKQAMMLVPEIALTPQMVLRFK
                     RRFGDDVAVLHSGLSNGERYDEWQKIRDDRARVSVGARSSVFAPFKNLGLIIIDEEHE
                     STYKQEDYPRYHAREIAQWRSEYHHCPVILGSATPCLESYARAEKDVYHLLSLPNRVN
                     QQALPEIDIVDMREELSEGNRSMFSKDLREAIQLRLDRQEQVVLFLNRRGYASFMLCR
                     DCGYVPQCPNCDISLTYHKTTDLLKCHYCGYQETPPNQCPNCESEHIRQVGTGTQKVE
                     ELLQQEFEDARIIRMDVDTTSKKGAHEKLLTEFEKGNGDILLGTQMIAKGLDYPNITL
                     VGVLNADTMLNLPDFRASERTYQLLTQVAGRAGRHEKAGQVIIQTYNPDHYSILDVQK
                     NDYLTFYRQEMEYRKLGKYPPYYYLINFTISHKEMKKVMEASQHVHKILLQHLTEKAL
                     VLGPSPAALARINNEFRFQILVKYKSEPGLLQAIQFLDDYYHEKFIKEKLALKIDIDP
                     QMMM"
BASE COUNT         7150 a         2935 c         3832 g         6296 t
ORIGIN      
        1 ctgagggagc agtgccagtc gaagaccgag gctgagacgg caccctagga agggacccat
       61 catcaaaaat tctatttata gaattttaca gtaatgtgcc agatgggcat agcgaagcca
      121 ttcaatacga agtattgtat aaatagagaa cagcagtaag ataattttta attagaaaat
      181 atcttactac tgtcttttta gggatttatg ttccagcctg ctttcctaat ttttaatgtc
      241 atcttaaata tgataaatga ataattaagt tcatatttaa tgtcaaaaca tagtagttta
      301 tcaagtattg agtgagtaac attagattta atgtaatatg cttacttttt ttattagcag
      361 gtgtaagcta taatataaag agttgtctta tggacgattg attggaggaa cgaaaatgca
      421 caaaaaatat tttattggca cttccatttt aatagcagta tttgtcgtta tatttgacca
      481 agttactaaa tatattatag ctactacaat gaaaattgga gattcatttg aagtgatacc
      541 gcacttttta aacataacat cacatcgaaa taatggtgct gcatggggaa tattgagtgg
      601 aaaaatgaca tttttcttta ttattaccat tattatatta atagccttag tatatttctt
      661 tattaaagat gctcaatata atttgtttat gcaagttgct attagtttac tttttgcagg
      721 tgcacttgga aactttattg atagaatttt aacaggagaa gttgttgact ttattgatac
      781 aaatattttt ggttatgatt ttccaatatt taatatagca gattcaagtt taacaattgg
      841 tgtaatatta attattattg ccttattaaa ggatacttcc aataaaaagg agaaggaggt
      901 taagtaatgg agacttatga atttaacatt acagataaag aacaaacagg tatgcgtgta
      961 gataagttgc tgcctgaatt aaataatgat tggtctcgta accagataca agattggatt
     1021 aaagcaggtt tagtcgttgc aaacgataaa gttgttaaat ctaattataa agtgaaactt
     1081 aatgatcata tagttgtcac tgaaaaagaa gtggttgaag ctgatattct acctgaaaat
     1141 ttaaatttag atatttatta tgaagatgac gatgttgcag ttgtatataa accgaaaggc
     1201 atggtagttc atccatcacc agggcattat accaatacat tagttaatgg tttaatgtat
     1261 caaattaaaa atttgtcagg tattaatgga gaaattcgtc caggtattgt tcaccgtata
     1321 gatatggata cttctggttt attaatggtt gctaaaaatg atattgctca tcgtgggctt
     1381 gtagaacaat taatggataa atctgttaaa agaaaatata tcgctttagt tcacgggaat
     1441 attcctcatg attacggtac aatcgatgcg ccaattggta gaaacaaaaa tgatcgtcaa
     1501 tctatggctg ttgttgatga tggtaaggaa gcagtgacac attttaacgt actagaacat
     1561 tttaaagatt atacgcttgt tgaatgtcaa cttgaaacag gacgtacgca tcaaatccgt
     1621 gtgcacatga aatatattgg cttcccatta gttggtgatc caaagtatgg accgaaaaag
     1681 acattggata ttggtggtca agctctacat gctggactta ttggattcga acatccagta
     1741 acaggtgaat atattgaaag acatgctgaa ttaccacaag actttgaaga tttattagat
     1801 acaattcgaa aaagagatgc ataattgtgt tgtgcgataa ttacgataac attattatgt
     1861 aaactataag tatttatcgt tttcgttttt ttaaaatgaa aacacattat aaaatagatt
     1921 aaggttaaca tttttactga gatcggttaa tttttcaata tagattgaca aagcgcctga
     1981 ttagtattat ccttataaca gtttaagtac aacataaaga gtctttaatt gagcgtccag
     2041 agagacgtca aagacatgaa aatatagata tatactaaaa tgatcataac actttcacat
     2101 atgcaagaat aagtagtaat actacgattc ttctatgtaa ataggctgat tgtatatact
     2161 aaacctcatg tcgatattaa agacatgagg ttttttgttt ctaaattttg aaagggtgag
     2221 aatatgtctg aacgtattat aatggatgat gccgcaatac aacgtacagt gacgagaatc
     2281 gctcacgaaa ttttggagta taacaaaggt actgataatt taattctttt aggtatcaaa
     2341 acaagaggtg aatatttagc gaatcgtata caagataaaa ttcatcaaat tgagcaacaa
     2401 cgtataccta ctggaacaat tgatattaca tactttagag atgatataga gcacatgtca
     2461 tcacttacga caaaagacgc aatagacatc gacacagata ttacagataa agtagtcatc
     2521 attattgatg atgtgctgta tactggtcga acggttcgtg cttcacttga tgctattttg
     2581 ctaaatgcta gacctattaa aattggttta gctgctttgg ttgatcgagg acatcgtgag
     2641 ttaccaattc gagcagattt tgttggtaaa aatataccta cttctaaaga ggaaacggta
     2701 agtgtctatt tagaagaaat ggatcaaaga aatgcagtta taattaaata ataccccttt
     2761 taattcagta cgagagactg aaaaaggtgg gggagttgaa tgataaataa gttttcatat
     2821 gcttgtttat ctaataatac cctcatgttc gacaactata agtcaatttc tatggacgaa
     2881 aatgacttgt agcaacttgt cacacttttt gtctctttac atatactgat gtaaagagat
     2941 ttttttatta tcggaggaaa tgaatattat gcaaaatgat gaaatgtttg aacgaacagt
     3001 aaaaccagta ctagatgtaa atgaaaaacc acaaccagcg caatgggcat ttttaagctt
     3061 acaacattta tttgcgatgt ttggcgcaac agtactagta ccactcttaa caggactacc
     3121 aatatccgca gcgttactag cttcgggaat cggtacatta ctttatatct taataacgaa
     3181 ggcgcaaata ccagcatact tgggatctag ctttgcattt atcacaccaa ttatcacggg
     3241 attaagtacg catagcttag gagacatgct tgtagcatta ttcatgagtg gtgttatgta
     3301 cgtcatcatc ggaattctaa tcaaattaag tgggacagca tggttaatga aattattacc
     3361 accagttgtt gttggaccag tcatcatggt aattggttta agcttagcgc ctactgcagt
     3421 caatatggca atgtatgaaa atcctggaga tatgaaaggt tacaatatca gtttcttaat
     3481 tgttgcaatg ataaccttgc ttgtaacaat agtcgttcaa ggatttttta aaggattcct
     3541 atctttaatt ccagtacttg taggtattat cgtaggttat gtggtagcga tttttatggg
     3601 gattgtgaaa tttgatgcaa ttatgtcagc aaaatggata gatttccctc atatttatct
     3661 gccatttaaa gattatgtac cttcatttca cttaggactt gtacttgtaa tgattccgat
     3721 tgtgtttgta acagtaagtg aacatattgg gcaccaaatg gtattgaata aaatcgtagg
     3781 tagaaacttc tttgaaaagc caggacttga taaatcaatc attggtgatg gtgtttctac
     3841 aatgtttgcc agtattattg gtggaccacc aagtacaaca tacggtgaaa atatcggtgt
     3901 attagcgatt acaagaatat acagtattta cgtcattggt ggtgcagcag ttatagcaat
     3961 tgttttagca ttcattggta agttcactgc attaatttct tctataccta caccagttat
     4021 gggaggagta tctatattac ttttcggtat tattgcagca agtggcttaa gaatgttagt
     4081 tgaaagcaaa gtagattttg cgaacaatcg aaatttagtt atagcttctg taattttagt
     4141 tgtaggtatc ggtaatttag tatttaactt aaaagaaatt ggtatcaacc ttcaaattga
     4201 ggggatggca ttagctgcac tttcaggaat tattttgaac ttaatcttac ctaaagagaa
     4261 aaaacaaaac aattaagatt tacaaattaa ggagggcgct tttatgaatc atttattatc
     4321 aatggaacat ttatctacag atcaaatata caaacttatc caaaaggcaa gtcaatttaa
     4381 atctggtgaa cgtcaactac caaactttga agggaaatat gtcgcaaatt tattctttga
     4441 aaattctact cgaacaaaat gtagttttga aatggcagaa cttaagctag ggttaaaaac
     4501 gattagcttt gaaacatcaa catcatctgt ttcaaaaggt gaatctttat atgacacatg
     4561 taaaacttta gaaagtattg gctgtgattt attagtcatt agacatccgt ttaataacta
     4621 ctatgaaaaa ttagcgaata ttaacatccc aattgcgaat gctggtgatg gtagtggaca
     4681 acatccaaca caaagtttac ttgatttaat gacgatatat gaagaatatg gatattttga
     4741 aggcttgaat gtattgattt gtggagacat taaaaattca cgtgtcgcac gtagtaatta
     4801 ccatagttta aaagcattag gtgcaaacgt aatgtttaat agcccaaatg cttggattga
     4861 tgattcttta gaagcacctt atgtaaatat agatgatgtt atagaaacag tagatatagt
     4921 tatgttatta agaattcaac atgaaagaca tgggcttgca gaagaaacta gatttgcagc
     4981 agatgattat catcaaaagc atggcttaaa tgaagtgcgc tataacaaat tacaagaaca
     5041 tgctattgtt atgcatccgg cacctgtgaa tagaggagta gaaatacaaa gcgatttagt
     5101 agaagcttca aaatcaagaa tttttaagca aatggaaaat ggcgtttact taagaatggc
     5161 agtcattgat gaattattaa aataggtaag gggacgaaaa tgatgaaatt aattaaaaac
     5221 ggtaaagtat tacaaaatgg agaattacaa caagcagata ttttaattga tggtaaggta
     5281 attaaacaaa ttgcacctgc aattgaacca agcaatggtg ttgacatcat agatgcgaaa
     5341 ggtcactttg tgtcacctgg atttgtcgat gttcatgttc atttacgtga acctggtggt
     5401 gaatataaag agacaattga aactggtact aaagctgctg ctagaggcgg atttacaact
     5461 gtatgtccaa tgcctaacac aagaccggta ccagattctg tagaacattt tgaagcttta
     5521 caaaaattaa tcgatgacaa tgctcaagta cgtgtattac cttatgcttc aattacaaca
     5581 cgtcaattag gtaaagaatt ggttgatttc ccagcactag taaaagaagg tgcctttgcg
     5641 tttacagatg acggtgtagg agtacaaact gcaagcatga tgtatgaagg catgattgaa
     5701 gctgcaaaag taaacaaagc catcgtagca cactgtgaag ataattcatt aatctatggt
     5761 ggtgcaatgc atgaagggaa acgcagtaaa gagttaggta taccaggtat tccaaacatt
     5821 tgtgaatctg ttcaaatcgc aagagatgta ctattagctg aagcagcagg ttgtcattat
     5881 catgtatgtc atgtttctac taaagaaagt gttagagtca ttcgtgacgc taaacgcgca
     5941 ggcattcatg ttacagctga agttacacca caccatttat tgttaacaga agatgatatt
     6001 cctggtaata atgccattta taaaatgaat ccaccattga gaagtactga agatagagag
     6061 gctttgttag aagggttact agacggtaca attgactgta tcgcaacaga ccatgcacca
     6121 catgcacgtg atgaaaaagc acaaccaatg gaaaaagcac cattcggaat tgttggtagt
     6181 gaaacagcat tcccattatt atatacgcat tttgtaaaaa atggtgattg gacattacaa
     6241 caattagtag attacttaac aattaaacca tgtgagacat ttaatttaga atacggcaca
     6301 ttaaaagaaa atggttatgc agatttaaca atcattgatt tagatagtga acaagaaatt
     6361 aaaggagaag atttcttatc aaaagcagat aatacaccat ttatcggcta taaagtttat
     6421 ggaaatccga tcttaacaat ggttgaaggc gaagttaaat ttgaggggga taaataatat
     6481 gcaaagcaaa cgttatctag tgttagaaga cggttctttt tacgaaggct accgtttagg
     6541 atctgataac ttaactgtag gagaaattgt atttaataca gctatgacag gttatcaaga
     6601 aactatttca gatccatcat ataaaggtca gatcattact tttacgtatc cattaatcgg
     6661 taattatggt atcaatagag acgattttga atcattagta cctacattaa acggtattgt
     6721 agtgaaagaa gcgagtgcgc atccaagtaa ttttagacaa caaaagacac ttcatgacgt
     6781 tttagaattg catcaaattc cagggattgc aggtgttgat acaagaagta ttacgcgtaa
     6841 aattcgacaa cacggtgtgt taaaagctgg ttttactgat cgaaaagaag atattgatca
     6901 acttgtcaaa catttacaac aagtagaatt acctaaaaac gaagtagaaa tcgtttcgac
     6961 taaaacaccg tatgtttcga caggtaagga tctaagtgtc gtacttgtag actttggtaa
     7021 gaagcaaaat attgttcgag aattaaacgt cagaggttgt aacgtcacag ttgtaccata
     7081 tacaactact gccgaagaaa ttttagcaat ggctccagat ggcgttatgc tatcaaacgg
     7141 accaggtaat cctgaagttg tagaatgtgc gattccaatg attcaaggaa ttttagggaa
     7201 aattccgttc tttggtatct gtctaggaca tcaacttttt gcattatctc aaggagcaag
     7261 ctcatttaaa atgaagtttg gtcatcgtgg tgcgaaccat ccagttaaaa atttagagac
     7321 tggaaaagtt gatattacga gtcaaaacca tggatatgca atagatatag attcgttaaa
     7381 aagtactgat ttagaagtta ctcatcttgc attaaatgat ggtactgtag aaggtttaaa
     7441 acataaaaca ttaccagcat tttctgttca ataccatcct gaagcaaatc caggaccgtc
     7501 agattcaaac tatctatttg atgattttgt agcaatgatg actaatttta aggaaaagga
     7561 gcgtcatatc aatgcctaaa cgtaatgata tcaaaacaat tttagtaata gggtctgggc
     7621 caattatcat aggtcaagca gctgaatttg attatgctgg aacacaagca tgtctagctt
     7681 taaaagaaga gggatatcga gttattcttg taaattcaaa tccagcgaca atcatgactg
     7741 ataaggaaat tgcggataaa gtatatatcg aaccgttaac tcatgatttt atagcgcgaa
     7801 ttatacgtaa agagcaacct gacgctttac ttccaacttt aggtggtcaa acaggtttaa
     7861 acatggcgat tcaactacac gaaagtggtg tgcttcaaga taataacgtc caattattag
     7921 gaactgagct aacatcaatt caacaagcag aagaccgtga aatgtttaga acattaatga
     7981 atgatttaaa cgttcctgta ccagagagtg acattgtaaa tacagtagag caagccttta
     8041 aattcaaaga gcaagtggga tacccgctaa ttgttagacc ggcatttacg atgggtggta
     8101 ccggaggcgg tatttgtcat aatgatgaag aattacatga aatcgtctca aatggtcttc
     8161 attatagtcc agcaacgcaa tgtttattag aaaaatctat cgcaggtttt aaagaaatcg
     8221 aatacgaagt aatgcgtgat aaaaacgata atgccatcgt tgtatgtaac atggaaaata
     8281 ttgatccagt tggtattcat actggcgatt caattgttgt ggctcctagt caaacattat
     8341 cagatgttga gtatcaaatg ttacgtgatg tttcattaaa agttattcga gctttaggta
     8401 tcgaaggtgg ttgtaatgtt caattagcat tagatcccca ttcattcgat tattatatta
     8461 tagaagtaaa tccgcgtgta tcacgttcat cagcgttagc ttcaaaagca acaggatatc
     8521 ctattgcaaa attagctgct aaaatcgcgg ttggtctaac attagatgaa atgttaaatc
     8581 caattacagg aacatcttat gcagcgtttg aaccaacttt agactatgtg atttcaaaaa
     8641 taccaagatt tccttttgat aaatttgaaa aaggagaacg agagcttggc acacaaatga
     8701 aagcaacagg tgaagttatg gccattggtc gaacttacga agaatcattg ttaaaagcaa
     8761 ttcgatcact tgagtatggt gtgcatcact taggattacc aaatggtgaa agcttcgatc
     8821 ttgattatat taaagaacgt atttcacacc aagatgatga acgattattt ttcatcggcg
     8881 aagcaattag aagagggaca acattagaag aaattcataa tatgactcag attgattact
     8941 tcttcttaca caagttccaa aacattattg atattgagca tcaattaaaa gagcatcaag
     9001 gtgatttaga atatcttaaa tatgcaaaag attatggatt tagtgataaa acaatagcgc
     9061 atcgctttaa tatgacggaa gaagaagtat atcaattgcg tatggaaaat gatattaaac
     9121 ctgtttacaa gatggttgat acttgcgcag ctgaatttga atcttcaaca ccatattatt
     9181 atggtacata cgaaactgaa aatgaatcca tagttactga caaagaaaaa atcttagtat
     9241 taggctctgg accaattcga atcggccaag gtgtagaatt tgactatgcg acagttcacg
     9301 ccgtttgggc aattcaaaaa gcagggtacg aagcgataat tgtgaataac aatccagaaa
     9361 cagtttcaac agacttctca atttctgaca aattatactt tgaaccttta actgaagaag
     9421 atgtgatgaa tatcattaat ttagaaaaac ctaaaggtgt cgttgtacaa tttggaggac
     9481 aaacagcgat taatttagca gacaaattgg ctaaacatgg tgttaaaata cttggtactt
     9541 cactagaaaa tctaaatcgt gctgaagata gaaaagaatt tgaagcacta ttaagaaaaa
     9601 ttaacgtgcc acagccacaa gggaaatcag ctacatcacc tgaggaagca ttagcgaatg
     9661 ctgcagaaat cggatatccg gttgtagtaa gaccttctta tgtattaggt ggtcgcgcaa
     9721 tggaaattgt agacaatgac aaagagttag aaaactatat gacccaggct gtaaaagcga
     9781 gtccggaaca tccggtacta gtcgatagat atttaactgg taaagaaatt gaagttgatg
     9841 cgatttgtga tggagaaacg gtcattattc caggaatcat ggaacatatt gaacgtgctg
     9901 gtgtacatag tggtgactca atcgctgtat atccgccaca aactttgaca gaagacgagt
     9961 tagcaacact tgaggactat actataaaat tagctaaagg tttaaacatc attggcttaa
    10021 tcaacattca attcgttata gctcacgatg gtgtatatgt tttagaagta aacccacgtt
    10081 ctagtagaac ggtaccattc ttaagtaaaa ttactgatat tccaatggca caattagcta
    10141 tgcgagcaat cattggggaa aaactaacag atatgggtta tcaagaaggg gttcaaccat
    10201 atgctgaggg tgtatttgtg aaagcaccgg tatttagttt taataaattg aaaaatgttg
    10261 atattacttt aggacctgaa atgaagtcta caggtgaagt aatggggaaa gatactacat
    10321 tagaaaaggc gttattcaaa gggttaacag gtagtggtgt tgaagttaaa gatcacggta
    10381 cagtattaat gaccgtcagt gacaaagata aagaggaagt tgttaaattg gcacaacgct
    10441 taaatgaagt tggctataaa attttagcaa cgtctggaac agctaataaa ttagctgagt
    10501 atgacatacc tgcagaagta gtaggcaaaa tcggtggcga aaatgattta ttaacacgta
    10561 ttcaaaatgg tgatgttcaa atcgttataa atacaatgac taaaggtaaa gaagtagaaa
    10621 gggatggctt ccaaattaga cgtactacag ttgaaaatgg tattccatgt ttgacatctt
    10681 tagatacagc taatgcctta acgaatgtaa ttgaaagtat gacatttaca atgcgtcaaa
    10741 tgtaaatcaa tcaaactgta tcggtggggc tgtaattaac catttactta aagaagttta
    10801 tattacagcc tcattatttt aatgaatttc ttaatataaa gggagacata tatgatgaaa
    10861 gatttaccaa ttattgcatt agattttgaa tcaaaagaaa aagtaaatca atttttagat
    10921 ttatttgatg aatcattatt cgtaaaagta ggtatggaac ttttttatca agaaggtcct
    10981 caattaatta atgagataaa agaaagaggc catgatgtat ttttagattt aaaactgcat
    11041 gatattccta atacagttgg taaggcgatg gaaggactag ctaaattgaa tgttgatctg
    11101 gtaaatgttc atgctgctgg tggcgtaaaa atgatgtctg aggccattaa aggattaaga
    11161 aaacataatc aacatacaaa aattattgca gtaacacagc ttacgtcaac aacagaagac
    11221 atgttacgac acgaacaaaa tatacaaaca tcgattgaag aggccgtttt aaattatgcc
    11281 aagttagcaa atgcagctgg tttagatggc gttgtttgtt cacctcttga aagtcgtatg
    11341 ttgactgaaa agttaggtac atcattttta aaagtaacac caggtattag acctaaaggt
    11401 gcatctcaag atgaccaaca ccgtattacg acaccggaag aagcaagaca gcttggttcg
    11461 acgcatattg tagtcggtag accgattaca caaagtgaca atccagtcga aagttatcat
    11521 aaaattaaag aaagttggtt agtataatgg ctaaagaaat tgcaaaatca ttattagata
    11581 ttgaagctgt aacattatca ccaaatgatt tatatacatg gagttcaggt attaaatcac
    11641 cgatttactg tgataaccgt gttacgttag gttatccttt agttcgaggc gcaatccgcg
    11701 atggtttaat taacttaatt aaagaacact ttcctgaagt agaagttatt tctggtactg
    11761 caacagctgg tattccacat gcagctttta ttgctgaaaa attaaaatta ccaatgaatt
    11821 atgttcgttc atcaaataag agtcatggta agcaaaatca aatcgaaggt gctaaaagtg
    11881 aaggtaaaaa agtagttgtg atagaagatt taatttcgac agggggatct tcagtcacag
    11941 cagttgaagc cttaaaacaa gcaggtgcag aagtattagg tgttgtagct atctttactt
    12001 acggtttgaa aaaagcagat gatacattta gcaatattca actacctttt tacactttaa
    12061 gtgattacaa tgaattaatt gaagtagctg aaaatgaagg taaaatttct agtgaagata
    12121 tccaaacatt agttgaatgg agagacaact tagcataata tagacactag aaggaggaat
    12181 tcaacaaatg aatgacaaaa catctaatga tttatatggg aagataaaac attgtaacga
    12241 atttatcaat cattcaaatg attccaatct atctagtagt cacgatgtcg acgaaagttc
    12301 aacgaagcaa aaacatataa aaaataaaac aactatagat cataatgatg atttatttaa
    12361 acatgtaaag gatatattac gtaaacaagg acaaatttaa aaacaaaaaa gctatagtgt
    12421 ttaatatcag gggctgctta accgcatccg aatattacaa acactatagc ttttattata
    12481 taaaaaattt attagcggat aattaacttt attaaaatcc aaccaattaa gaatacgagt
    12541 aataggacta atactggaat cacataatgt aacataacgt ccctccttta acttaatttt
    12601 aattgtaatc aaatttgaca ataagtcaaa acattaatac ctatgataag tatcatttat
    12661 taacatatgt atcatatttt taatcttgcg taatttttat cgttaactat gtgatttaat
    12721 caacaggcca ataagacgtt tgtcattcgt gcacataacg gtatgacgcg cttttgtcat
    12781 tttaattatg ttaagataat agtagttata gaagttcgat ctataggagg catagcatgg
    12841 atattccaaa aatcacgaca tttttaatgt ttaataacaa agctgaagaa gctgttaaac
    12901 tatacacaag cttatttgaa gatagtgaga ttataacaat ggctaagtat ggtgaaaatg
    12961 gacctggtga tcacgggact gtacaacact caatatttac attaaatgga caagtattca
    13021 tggcgattga tgctaatagt ggcacagaat taccaatgaa tcctgcgatt tcattatttg
    13081 ttacagtaaa agatactatt gaaatggaac gactatttaa tggattaaaa gatgaaggtg
    13141 ccattttaat gccaaaaacg aatatgccac catacagaga gtttgcttgg gttcaagata
    13201 agtttggagt aagttttcaa ttagcattac ctgagtaaaa ggattcgcac agcgttgaat
    13261 gataaagaaa cactttttct tatgcatgcg ttttacctat gtatttctat tttaagtaca
    13321 cattagcatt ttagtttcac tcatttttaa aatcacaaag ataatttgta acttaaatga
    13381 tgatacgtta tttaaaaaca cgatacttcg tttcaatgaa cgcattaaat aataaataaa
    13441 cacctcgcaa aagaaagtag tcctttctta tggtgaggtg tttatttgtc gacaacttga
    13501 ttataattgt ttcatttttt gaataagttc ataatcaggt gtagcataca aagttttttg
    13561 attgtcatat gttacaaacc caggctttgc acctgatggt ttgtgcacat ttttaattaa
    13621 tgtgtaatca acaggtattt gtccagaatt accagctttt gaaaagtatc ctgctaacat
    13681 agccgcttcc ttgattgtcg tatcacttgg tgcatcatta aatataacga catgtgaacc
    13741 aggaatatct tttgtgtgta accatgtgtg agttttttta gcttttttat ttgttaaata
    13801 atcattttgc ttgttattct tgccaacata tatatcgtcg ccatcagttg atacataatg
    13861 ttgtaattga atctgcgctt tctttttctt agtttgattt ttacgctgtt tcataaagcc
    13921 ttgttctgct aattcatctc taatttcatc aatgtcatgg acagaaatat gatgtaattg
    13981 ttgttcgatt gttgaaaaat aatctatatt atctttcgtc aattgaattt gatgttgtaa
    14041 ttcacgttct ctcgttttca tacgattata ttgtttataa taatattgag catttgctga
    14101 tggggatttt gtaggattta aaggaatgac aacttcttca ttcgtataat aattcaatgc
    14161 tgtcacttct ttatcgcctt gcttaattcg atatatatta gcagtgatca attcaccata
    14221 taactgttcg gtatctttat ttttagactg ctcatattct tcaatcaact tcgctaattt
    14281 attttgatat ttgtgcaact gctgttgaac aaatcgaact aaatcattcg cacgttgttt
    14341 aacgcgttca cgttcgccac gcgcgtcata aaaacgatca agtaaatcat ttaatgaatc
    14401 gtacgtaact gtatcatcat taaattgatt taactttata aaatagaaat cctctttacc
    14461 tgtttcatga tttttatgaa aaataggagt aggtggtaac ttggtttctg ccattacttc
    14521 gtcgaatgct tctggtaatg ttgatgaagt cataaattga cgacgactaa cgatttcatt
    14581 cgtaattaaa gggctaaatc cttcaaactg attcaataat tgtttagcaa tattacctgc
    14641 gttaaaatcg atatatttca acacctctgc acctgtaata tcatacggat ttattttgtg
    14701 ctgagtaggt ggtgcttcat aattaaatcc tggcattact gtacgatagt gattcgtatt
    14761 tggtgttaag tgtttaaatc cttcaattat tttgcgattt tcatctacta aaattaagtt
    14821 actatgttta cccataatct caaggatgac agtgcggtaa atagtatcgc caatttcatc
    14881 tttactcttt atatcgattt caatgcgacg atcattacca atttgcttaa tcgattcgat
    14941 aataccacct tctaagtgtt ttctaaaaac acgcgcaaac atgggtggat taaatggatt
    15001 atcatatttt ttagtagtca attgtaatct tgaaaagttt ggatggattg acaataacaa
    15061 ttgatggttt tgtctatttt gacgtacaac cattagtatc gtgtcattat caggttgatt
    15121 gattttgtga acgcgtcctg ttgttaaaaa ttgtagagac tcaaccattt tctttgtaaa
    15181 taatccatca taagccataa tttatcttcc accttcttca tatcattcgt ttcattttaa
    15241 catgattgta acttttcggt atagttattg aaccatacat tatgccacac attttacatt
    15301 atctaaaagt tcgcaattca atttatttaa ttaaaattaa tcgtgcaata taacgatatt
    15361 gttagactta atagaaatta tggcatgcaa tttcaaatat gctatacaat ataaagaaca
    15421 atgtgatatc atatttaaat aatagaagat tagcttagag aggtcgtaag gcatggataa
    15481 tgaaaaagga ttgttaatcg ttttatcagg accatctgga gtaggtaaag gtactgttag
    15541 aaaacgaata tttgaagatc caagtacatc atataagtat tctatttcaa tgacaacacg
    15601 tcaaatgcgt gaaggtgaag ttgatggcgt agattacttt tttaaaacta gggatgcgtt
    15661 tgaagcttta attaaagatg accaatttat agaatatgct gaatatgtag gcaactatta
    15721 tggtacacca gttcaatatg ttaaagatac aatggacgaa ggtcatgatg tatttttaga
    15781 aattgaagta gaaggtgcaa agcaagttag aaagaaattt ccagatgcgt tatttatttt
    15841 cttagcacct ccaagtttag atcacttgag agagcgatta gtaggtagag gaacagaatc
    15901 caatgagaaa atacaaagtc gtattaacga agcgcgtaaa gaagttgaaa tgatgaattt
    15961 atacgattac gttgtagtta atgatgaagt agaacttgcg aagaatagaa ttcaatgtat
    16021 tgtagaagct gagcacttaa aaagagagcg cgtagaagct aagtatagaa aaatgatttt
    16081 ggaggctaaa aaataatgtt aaatccacct ttaaaccaat taacgtcaca aattaaatca
    16141 aagtatttaa ttgcaacaac tgcagcgaaa agagcgcgtg aaattgatga acaacctgaa
    16201 actgaattat taagtgaata tcattcattt aaaccagttg gtagagcgtt agaagaaatt
    16261 gctgacggta aaattcgccc tgttatttca agtgattatt atggtaaaga atagtttcga
    16321 ccattaaaaa tatgtgaagc tagacgtaca tcaaatacgt caaggcatat ccaaatcaaa
    16381 cgcacctatg taggtgtgtt tttttagttt atccaatgat tgatgttata ataatactaa
    16441 atttgtatct ataaaaaagt aatgagcatt tgtgcgcata tgatgatgta aagcgtaaat
    16501 gtcatagtga tatttaaggg agattgaata tgaagaaaat attattagcc gttacaggtg
    16561 gcattgcggc atataaagca attgatttga caagtaagtt aacacaatct gggtatgaag
    16621 ttcgcgttat gttaacgaat cacgcacaaa aatttgtgac accattagca tttcaagcaa
    16681 taagtcgaaa tgctgtttat acagatactt ttatagaaga aaatccttca gaaatacagc
    16741 atattgcatt aggtgattgg gcagatgcaa tcattgttgc acctgcaacg gcaaatacaa
    16801 ttgcaaaatt gagtgtaggt attgctgatg atttggtgac atcaacgttg ctagcaacag
    16861 agacaccgaa atttattgcg cctgctatga atgtgcatat gtatgaaaat aaacgtacgc
    16921 agcaaaatat taatatttta aaagaagatg ggtatcattt tatcgaacca ggaagcggat
    16981 ttctagcatg tggttatgtt gctaaaggac gtatggaaga accgcttcaa atcgtttctg
    17041 ttattgatgc tcattttcaa aatagtaatc gtttagctaa tagttcattt caagataaac
    17101 gcgcattggt tacagcagga ccaactattg aagttatcga tccagtcaga tttgtatcca
    17161 atcgttcttc tggaaaaatg ggctatgcaa tagctgaagc attgcgaaat cgaggagcta
    17221 tcgtgacgtt agttgctggt cctacaacac tagaggatcc aaaagatatt gaagttattc
    17281 atgttcaaag tgctgaagaa atgtttgaac aagtgacaag ccgatttgac gaacaagata
    17341 ttgttgtaaa agcagcagcc gtatctgact atacaccggt tgatgtatta gaacataaga
    17401 tgaaaaagca ggatggtgat ttgtcagtat cttttaaacg tactaaagac attcttaaat
    17461 atttaggtga acataaaaca tcacagtatt taataggctt tgcagcagag actgaagata
    17521 ttgaaaatta tgcacaacaa aaattacgca agaaaaatgc agatgtgatt atttcaaata
    17581 atgttgggga tatgtctatc ggatttagtt ctgatgataa tgaattgaca atgcatttta
    17641 aaaataatga aaaggtaaat atcaagaaag gaaaaaaagt agtattagct gcacaaattt
    17701 tagatgaact agaaactagg tggcaataat gatagcgaaa gtcatagtcg atgtcgcgtc
    17761 gaagagcgtt gactataaat ttgattatat aattcccgaa caactcgaat ctgtcatcca
    17821 acctggtgtg cgtgtaattg taccttttgg accaagaacg attcaaggtt atgtaatgga
    17881 agtaacagca gaacctgatg cacaacttga cgtttcgaag ttaaaaaaaa tcatagaagt
    17941 gaaagatata caaccagaat taacatcaga attaatagct ttaagtgagt ggatgggttc
    18001 aactcatgtc attaaacgta tttctatgct agaagtgatg cttccaagtg ctattaaagc
    18061 gaagtataaa aaagcattta agatgaaaga tgacatagag ctaccttcag ctttattaca
    18121 aaaatttgat aagcatggtt actattatta taaagatgcg caaaaaaata atgatattca
    18181 attgcttatg aagttgttaa aagatgatat cgttgaagaa aaaacgattc tcacacaaaa
    18241 tataactaaa aaaaccaagc gtgctgttcg tgtcattgaa gggtatcatc ctgatgaagt
    18301 attagctaag ttggagaaag ttattaaaca atacgatttg tatgcttact tgtctgaaga
    18361 acaacataaa acaatatttt taactgatat tgaggatatg ggcttttcaa aatccagttt
    18421 agatggactt atcaaaaaag gttatgttga aaaatatgac gcggttgttg aaagagaccc
    18481 atttaaagat cgtgttttcg aacaagaatc aaaacagcaa ttaacagaag accaatataa
    18541 agcatatgaa gcgattaaag ctaaaattgt aagccaagag caagaaacat ttttacttca
    18601 tggtgtgacg ggatcaggta aaacagaagt atatttacaa acgatagaag atgttttaag
    18661 ccaaggaaaa caggcgatga tgttagttcc tgaaatcgct ctaacaccgc aaatggtttt
    18721 acgcttcaaa cgtcgatttg gtgatgacgt tgctgtatta cattctggct tatctaatgg
    18781 ggaacgttat gatgagtggc aaaaaattag ggatgatcgt gcgagagtaa gtgttggtgc
    18841 aaggtcaagt gtgttcgcac ctttcaaaaa tttagggtta atcatcattg atgaagaaca
    18901 tgaatctaca tataaacaag aagattatcc gagatatcac gctagagaaa ttgcccaatg
    18961 gcgaagtgaa tatcatcact gtccagtcat tttaggaagt gcaacaccat gtcttgaaag
    19021 ttatgcacga gctgaaaaag acgtttatca tttgctatca ttaccaaaca gagtgaacca
    19081 acaagcttta cctgaaattg atatagtaga catgcgtgaa gaattgagtg aaggtaatcg
    19141 gtcaatgttt tcaaaagatt tacgtgaagc catacaatta agattagatc gacaggaaca
    19201 agttgtttta tttttaaatc gacgtggtta tgcatcgttt atgttatgtc gggattgtgg
    19261 atatgtaccg caatgtccaa actgtgatat ttcattaacg tatcataaaa cgacagactt
    19321 attaaaatgt cactattgtg gttaccaaga gacgccaccg aatcaatgtc caaattgtga
    19381 gagtgaacac attcgacaag taggtactgg tactcagaaa gttgaagaac tattgcaaca
    19441 agaatttgaa gatgcgcgca taattaggat ggatgtagat acaacctcaa agaaaggtgc
    19501 acatgaaaag ttattgactg aattcgaaaa aggtaacggt gacattttac taggtactca
    19561 gatgattgcg aaaggattag attatccaaa tattacttta gttggtgtgc tgaatgcaga
    19621 tacaatgtta aatttacctg attttcgggc gagcgaacgt acttatcaac tattaacgca
    19681 agtggctggt agagctggtc gtcatgaaaa ggcaggtcaa gtcatcattc aaacgtataa
    19741 tccagatcat tattcaatat tggatgttca aaaaaatgat tatttaacat tttatcgtca
    19801 ggaaatggaa tatcgtaaat taggaaagta tccaccgtat tattatttga ttaatttcac
    19861 aatctcacat aaagaaatga agaaggttat ggaagcatcg cagcatgttc ataaaatttt
    19921 attacagcat ttaacagaaa aagcgcttgt actaggtcca tctccggcag cacttgcgag
    19981 aatcaacaat gaatttagat tccaaatttt agtgaaatat aaaagtgaac ctggattatt
    20041 acaagccatt cagtttttag atgactatta ccatgaaaaa tttataaaag aaaaattagc
    20101 attgaagatt gatattgatc cacagatgat gatgtaacat tactaattat tagaaacaag
    20161 ttcagtatgt acgagtattt gaaccagtgt ttgaatattt actttaagta cag
//
LOCUS       DAHHSC010000026        20101 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.026, whole genome shotgun sequence.
ACCESSION   DAHHSC010000026 DAHHSC010000000
VERSION     DAHHSC010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 20101)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20101)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20101
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.026"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            16..1126
                     /gene="prfB"
                     /locus_tag="NBI57_000746"
     CDS             join(16..87,89..1126)
                     /gene="prfB"
                     /locus_tag="NBI57_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014667688.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HCD1821712.1"
                     /translation="MELSEIKRNIDKYNQDLTQIRGSLDLENKETNIQEYEEMMAEPN
                     FWDNQTKAQDIIDKNNALKAIVNGYKTLQAEVDDMDATWDLLQEEFDEEMKEDLEQEV
                     INFKAKVDEYELQLLLDGPHDANNAILELHPGAGGTESQDWANMLFRMYQRYCEKKGF
                     KVETVDYLPGDEAGIKSVTLLIKGHNAYGYLKAEKGVHRLVRISPFDSSGRRHTSFAS
                     CDVIPDFNNDEIEIEINPDDITVDTFRASGAGGQHINKTESAIRITHHPSGIVVNNQN
                     ERSQIKNREAAMKMLKSKLYQLKLEEQAREMAEIRGEQKEIGWGSQIRSYVFHPYSMV
                     KDHRTNEETGKVDAVMDGDIGPFIESYLRQTMSHD"
     gene            1280..1387
                     /locus_tag="NBI57_000747"
     CDS             1280..1387
                     /locus_tag="NBI57_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1821713.1"
                     /translation="MCLLKQHMQCAFSNITYGDFQGIELIVENVLNYMV"
     gene            1535..2374
                     /locus_tag="NBI57_000748"
     CDS             1535..2374
                     /locus_tag="NBI57_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HCD1821714.1"
                     /translation="MKKTLTVTVSSVLAFLALNNAAHAQQHGTQVKTPVQHNYVSNVQ
                     AQTQSPTTYTVVAGDSLYKIALEHHLTLNQLYSYNPGVTPLIFPGDVISLVPQNKVKQ
                     TKAVKSPVRKASQAKKVVKQPVQQASKKVVVKQAPKQAVAKTVNVAYKPAQVQKSVPT
                     VPVAHNYNKSVANRGNLYAYGNCTYYAFDRRAQLGRSIGSLWGNANNWNYAAKVAGFK
                     VDKTPEVGAIFQTAAGPYGHVGVVESVNPNGTITVSEMNYAGFNVKSSRTILNPGKYN
                     YIH"
     gene            2548..3198
                     /locus_tag="NBI57_000749"
     CDS             2548..3198
                     /locus_tag="NBI57_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HCD1821715.1"
                     /translation="MGVHQYFKRLSDMERLIRLPGKFKYFEHNVAAHSFKVTKIAQYL
                     ATVEEYHGRKINWKSLYEKALNHDFAEVFTGDIKTPVKYASSELKKLFSQVEEEMVET
                     FIEEEIPLQYRDVYKQRLQEGKDDSLEGQILSVADKIDLLYETFGEIQKRNPEELFFE
                     IYEMSLETIIQFDHLASVQDFINNIIPEMLTENFIPRTELRETTMNILNKRKEENE"
     gene            3195..3431
                     /locus_tag="NBI57_000750"
     CDS             3195..3431
                     /locus_tag="NBI57_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbA family protein"
                     /protein_id="HCD1821716.1"
                     /translation="MIWYFSAAFFPCVLVVLFSVITRSKWVGTILTLILIGASIYKEY
                     FHNEWIIFIDVVSLLAGYLIIDQLEFHKHQDEDR"
     gene            3700..5685
                     /gene="uvrB"
                     /locus_tag="NBI57_000751"
     CDS             3700..5685
                     /gene="uvrB"
                     /locus_tag="NBI57_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002473941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HCD1821717.1"
                     /translation="MVEHYPFKIHSDFEPQGDQPQAIKEIVDGIKAGKRHQTLLGATG
                     TGKTFTMSNVIKEVGKPTLIIAHNKTLAGQLYSEFKEFFPENRVEYFVSYYDYYQPEA
                     YVPSTDTFIEKDASINDEIDQLRHSATSALFERDDVIIIASVSCIYGLGNPEEYKDLV
                     VSVRVGMEMDRSELLRKLVDVQYTRNDIDFQRGTFRVRGDVVEIFPASKEELCIRVEF
                     FGDEIDRIREVNYLTGEVLKEREHFAIFPASHFVTREEKLKVAIERIEKELEERLKEL
                     RDENKLLEAQRLEQRTNYDLEMMREMGFCSGIENYSVHLTLRPLGSTPYTLLDYFGDD
                     WLVMIDESHVTLPQVRGMYNGDRARKQVLVDHGFRLPSALDNRPLKFEEFEEKTKQLV
                     YVSATPGPYEIEHTDKMVEQIIRPTGLLDPKIEVRPTENQIDDLLSEIQTRVERNERV
                     LVTTLTKKMSEDLTTYMKEAGIKVNYLHSEIKTLERIEIIRDLRMGTYDVIVGINLLR
                     EGIDIPEVSLVVILDADKEGFLRSNRSLIQTIGRAARNDKGEVIMYADKMTDSMKYAI
                     DETQRRREIQMKHNEKHGITPKTINKKIHDLISATVENDENNDKAQTVIPKKMTKKER
                     QKTIDNIEKEMKQAAKDLDFEKATELRDMLFELKAEG"
     gene            5693..8539
                     /gene="uvrA"
                     /locus_tag="NBI57_000752"
     CDS             5693..8539
                     /gene="uvrA"
                     /locus_tag="NBI57_000752"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HCD1821718.1"
                     /translation="MKEPSIVVKGARAHNLKDIDIELPKNKLIVMTGLSGSGKSSLAF
                     DTIYAEGQRRYVESLSAYARQFLGQMDKPDVDTIEGLSPAISIDQKTTSKNPRSTVAT
                     VTEIYDYIRLLYARVGKPYCPNHNIEIESQTVQQMVDRIMELEARTKIQLLAPVIAHR
                     KGSHEKLIEDIGKKGYVRLRIDGEIVDVNDVPTLDKNKNHTIEVVVDRLVVKDGIETR
                     LADSIETALELSEGQLTVDVIDGEDLKFSESHACPICGFSIGELEPRMFSFNSPFGAC
                     PTCDGLGQKLTVDVDLVVPDKDKTLNEGAIEPWIPTSSDFYPTLLKRVCEVYKINMDK
                     PFKKLTERQRDILLYGSGDKEIEFTFTQRQGGTRKRTMVFEGVVPNISRRFHESPSEY
                     TREMMSKYMTELPCETCHGKRLSREALSVYVGGLNIGEVVEYSISQALNYYKNIDLSE
                     QDQAIANQILKEIISRLTFLNNVGLEYLTLNRASGTLSGGEAQRIRLATQIGSRLTGV
                     LYVLDEPSIGLHQRDNDRLINTLKEMRDLGNTLIVVEHDDDTMRAADYLVDIGPGAGE
                     HGGQIVSSGTPQKVMKDKKSLTGQYLSGKKRIDVPEYRRPASDRKISIRGARSNNLKG
                     IDVDIPLSIMTVVTGVSGSGKSSLVNEVLYKSLAQKINKSKVKPGLYDKIEGIDQLDK
                     IIDIDQSPIGRTPRSNPATYTGVFDDIRDVFAQTNEAKIRGYQKGRFSFNVKGGRCEA
                     CKGDGIIKIEMHFLPDVYVPCEVCDGKRYNRETLEVTYKGKNIADILEMTVEEATQFF
                     ENIPKIKRKLQTLVDVGLGYVTLGQQATTLSGGEAQRVKLASELHKRSTGKSIYILDE
                     PTTGLHVDDISRLLKVLNRLVENGDTVVIIEHNLDVIKTADYIIDLGPEGGSGGGTIV
                     ATGTPEDIAQTKSSYTGKYLKEVLERDKQNTEDK"
     gene            9203..10135
                     /locus_tag="NBI57_000753"
     CDS             9203..10135
                     /locus_tag="NBI57_000753"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HCD1821719.1"
                     /translation="MLTTEKLVETLKLDLIAGEEGLSKPIKNADISRPGLEMAGYFSH
                     YASDRIQLLGTTELSFYNLLPDKDRAGRMRKLCRPETPAIIVTRGLQPPEELVEAAKE
                     LNTPLIVAKDATTSLMSRLTTFLEHALAKTTSLHGVLVDVYGVGVLITGDSGIGKSET
                     ALELVKRGHRLVADDNVEIRQINKDELIGKPPKLIEHLLEIRGLGIINVMTLFGAGSI
                     LTEKRIRLNINLENWNKQKLYDRVGLNEETLSILDTEITKKTIPVRPGRNVAVIIEVA
                     AMNYRLNIMGINTAEEFSERLNEEIIKNSHKSEE"
     gene            10141..10980
                     /locus_tag="NBI57_000754"
     CDS             10141..10980
                     /locus_tag="NBI57_000754"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HCD1821720.1"
                     /translation="MGIVFNYIDPVAFNLGPLSVRWYGIIIAVGILLGYFVAQRALVK
                     AGLHKDTLVDIIFYSALFGFIAARIYFVIFQWPYYAENPSEIIKIWHGGIAIHGGLIG
                     GFIAGVIVCKVKNLNPFQIGDIVAPSIILAQGIGRWGNFMNHEAHGGPVSRAFLEQLH
                     LPNFIIENMYINGQYYHPTFLYESIWDVAGFIILVNIRKHLKLGETFFLYLTWYSIGR
                     FFIEGLRTDSLMLTSNIRVAQLVSILLILISISLIVYRRIKYNPPLYSKVGALPWPTK
                     KVK"
     gene            10988..11473
                     /locus_tag="NBI57_000755"
     CDS             10988..11473
                     /locus_tag="NBI57_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HCD1821721.1"
                     /translation="MRKFLSKTHHHTNPLWRVYRLVKFSKVFKNVIIIEFSKFIPSMV
                     LKRHIYKQLLNINIGNQSSIAYKVMLDIFYPELITIGSNSVIGYNVTILTHEALVDEF
                     RYGPVTIGSNTLIGANATILPGITIGDNVKVAAGTVVSKDIPDNGFAYGNPMYIKMIR
                     R"
     gene            11481..12920
                     /locus_tag="NBI57_000756"
     CDS             11481..12920
                     /locus_tag="NBI57_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HCD1821722.1"
                     /translation="MAQKNNNVIPMTFDDAFYRKMAKQKFKQREYKRAAEYFEKVLEL
                     SPDDLEIQIDYAQCLVQLGIAKKAEHLFYDNIIYNRHLEDSFYELSQLNIEVNEPNKA
                     FLFGINYVIVSDDQDYRDELDQMFDVKYQSEEQIELEAQLFVVQILFQYLFSQGRLKD
                     AKNYVLHQPQEVQDHRVVRNLLAMCYLYLGEYDTAKALYEALLQEDSTDIYALCHYTL
                     LLYNTKENEQYQKYLKILNKVVPMNDDESFKLGIVLSYLKQYRASQQLLYPLYKKGKF
                     LSIQMYNALAYNYYYLGEEDESHYYWDKLKQISKVEIGHAPWVIENSKEVFDQHILPL
                     LQSDDSHYRLYGIFLLDQLNGKEIVMTESIWQVLENLNNYEKLYLTYLVQGLTLNKLD
                     FIHRGLLTLYQNELFVSENDLMVAWINQGELIIAEKVDLTDVEPYIGAFIYLYFKNQP
                     RNVTKKQITTWLGITQYKLNKMIEFLLSI"
     gene            12987..13922
                     /gene="trxB"
                     /locus_tag="NBI57_000757"
     CDS             12987..13922
                     /gene="trxB"
                     /locus_tag="NBI57_000757"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HCD1821723.1"
                     /translation="MTEIDFDIAIIGAGPAGMTAAVYASRANLKTVMIERGIPGGQMA
                     NTEEVENFPGFEMITGPDLSTKMFEHAKKFGAVYQYGDIKSVEDKGEYKVINFGNKEL
                     TAKAVIIATGAEYKKIGVPGEQELGGRGVSYCAVCDGAFFKNKRLFVIGGGDSAVEEG
                     TFLTKFADKVTIVHRRDELRAQRILQDRAFKNDKIDFIWSHTLKSINEKDGKVGSVTL
                     TSTKDGSEETHEADGVFIYIGMKPLTAPFKDLGITNDVGYIVTKDDMTTSVPGIFAAG
                     DVRDKGLRQIVTATGDGSIAAQSAAEYIEHLNDQA"
     gene            14807..15718
                     /gene="rapZ"
                     /locus_tag="NBI57_000758"
     CDS             14807..15718
                     /gene="rapZ"
                     /locus_tag="NBI57_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HCD1821724.1"
                     /translation="MDNNEKEKSKSELLVVTGLSGAGKSLVIQCLEDMGYFCVDNLPP
                     VLLPKFVELMEQGNPSLRKVAIAIDLRGKELFNSLVAVVDKVKSESDVIIDVMFLEAS
                     TEKLISRYKETRRAHPLMEQGKRSLINAINDEREHLSQIRSIANFVIDTTKLSPKELK
                     ERIRRYYEDEEFETFTINVTSFGFKHGIQMDADLVFDVRFLPNPYYVVDLRPLTGLDK
                     DVYNYVMKWKETEIFFEKLTDLLDFMIPGYKKEGKSQLVIAIGCTGGQHRSVALAERL
                     GNYLNEVFEYNVYVHHRDAHIESGEKK"
     gene            15715..16710
                     /locus_tag="NBI57_000759"
     CDS             15715..16710
                     /locus_tag="NBI57_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HCD1821725.1"
                     /translation="MRQIKVVLIGGGTGLSVMARGLREFPIDITAIVTVADNGGSTGK
                     IRDEMDIPAPGDIRNVIAALSDSESVLSQLFQYRFEENQISGHSLGNLLIAGMTNITN
                     DFGHAIKALSKILNIKGRVIPSTNTSVQLNAVMEDGEIVFGETNIPKKHKKIDRVFLE
                     PNDVQPMEEAIDALREADLIVLGPGSLYTSVISNLCVNGISDALIHSDAPKLYVSNVM
                     TQPGETDGYSVKDHIDAIHRQAGQPFIDYVICSTQTFNAQVLKKYEEKHSKPVEVNKA
                     ELEKESINVKTSSNLVEISENHLVRHNTKVLSTMIYDIALELISTIPFVPSDKRK"
     gene            16821..17765
                     /gene="whiA"
                     /locus_tag="NBI57_000760"
     CDS             16821..17765
                     /gene="whiA"
                     /locus_tag="NBI57_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HCD1821726.1"
                     /translation="MSFASEMKNELTRIDVDEMNAKAELSALIRMNGALSLSNQQFVI
                     NVQTENATTARRIYSLIKRVFNVEVEILVRKKMKLKKNNIYICRTKMKAKEILDELGI
                     LKDGIFTHEIDHSMIQDDEMRRSYLRGAFLAGGSVNNPETSSYHLEIFSQNESHAEGL
                     TKLMNSYELNAKHLERKKGSITYLKEAEKISDFLSLIGGYQALLKFEDVRIVRDMRNS
                     VNRLVNCETANLNKTVSAAMKQVESIKLIDKEIGIENLPDRLREIARIRVEHQEISLK
                     ELGEMVSTGPISKSGVNHRLRKLNDLADKIRNGEQIEL"
     gene            18329..18916
                     /gene="clpP"
                     /locus_tag="NBI57_000761"
     CDS             18329..18916
                     /gene="clpP"
                     /locus_tag="NBI57_000761"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HCD1821727.1"
                     /translation="MNLIPTVIETTNRGERAYDIYSRLLKDRIIMLGSQIDDNVANSI
                     VSQLLFLQAQDSEKDIYLYINSPGGSVTAGFAIYDTIQHIKPDVQTICIGMAASMGSF
                     LLAAGAKGKRFALPNAEVMIHQPLGGAQGQATEIEIAANHILKTREKLNRILSERTGQ
                     SIEKIQKDTDRDNFLTAEEAKEYGLIDEVMVPETK"
     gene            complement(19159..20061)
                     /locus_tag="NBI57_000762"
     CDS             complement(19159..20061)
                     /locus_tag="NBI57_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HCD1821728.1"
                     /translation="MKQYLITGGTGMVGSQLVNEIKKSDSHITILTRHDQISNDKKIS
                     YVNWAKSGWEHKVPQNIDVVINLAGATLNKRWTPEYKQTLMLSRIQSTQALYELFKSR
                     NKAPKVLFNASATGYYPPDLFMSYTEVYKTLPFDFLSDIVYQWERFAQQFEQLGTRVV
                     IGRFGMILSNEGGALQTMKLPYKYYIGGKLGSGQQWYSWIHINDLIQAILFLINNESA
                     SGPFNLTAPIPERQNLFGYTLARAMHKPHETWAPSLAMRLILGQMSTVVLDTQKVLPN
                     KIQALGFQFKYSNLKMALEDLIKE"
BASE COUNT         7233 a         2795 c         3891 g         6182 t
ORIGIN      
        1 ataggagcga aggctatgga attatcagaa atcaaacgaa atatagataa gtataatcaa
       61 gatttaacac aaattagggg gtctctttga cttagagaac aaagaaacta atatccaaga
      121 atatgaagaa atgatggcag aacctaattt ttgggataac caaacgaaag cgcaagatat
      181 tatagataaa aataatgcgt taaaagcaat agttaatggt tataaaacac tacaagcaga
      241 agtagatgac atggatgcta cttgggattt attacaagaa gaatttgatg aagaaatgaa
      301 agaagactta gagcaagagg tcattaattt taaggctaaa gtggatgaat acgaattgca
      361 attattatta gatgggcctc acgatgccaa taacgcaatt ctagagttac atcctggtgc
      421 aggtggcacg gagtctcaag attgggctaa tatgctattt agaatgtatc aacgttattg
      481 tgagaagaaa ggctttaaag ttgaaactgt tgattatcta cctggggatg aagcggggat
      541 taaaagtgta acattgctca tcaaagggca taatgcttat ggttatttaa aagctgaaaa
      601 aggtgtacac cgactagtac gaatttctcc atttgattca tcaggacgtc gtcatacatc
      661 atttgcatca tgcgacgtta ttccagattt taataatgat gaaatagaga ttgaaatcaa
      721 tccggatgat attacagttg atacattcag agcttctggt gcaggtggtc agcatattaa
      781 caaaactgaa tcggcaatac gaattaccca ccacccctca ggtatagttg ttaataacca
      841 aaatgaacgt tctcaaatta aaaaccgtga agcagctatg aaaatgttaa agtctaaatt
      901 atatcaatta aaattggaag agcaggcacg tgaaatggct gaaattcgtg gcgaacaaaa
      961 agaaatcggc tggggaagcc aaattagatc atatgttttc catccatact caatggtgaa
     1021 agatcatcgt acgaacgaag aaacaggtaa ggttgatgca gtgatggatg gagacattgg
     1081 accatttatc gaatcatatt taagacagac aatgtcgcac gattaatata tattttaaaa
     1141 ccgaggctct aaaagggcgt cggtttttgg tttttttaaa ggtagctaaa taaattgtaa
     1201 attagatttt ggaatatgat ttgtttatga atatttaagt acaattcggt agatagagtt
     1261 agaatatatt ttttaaaagt tgtgtttgtt aaaacaacat atgcagtgtg catttagtaa
     1321 tattacctat ggcgattttc aaggtattga attaatcgtt gaaaacgttc tcaattacat
     1381 ggtatgaata cattttacac tatgataaaa ggttgtattc tttttatatt gttaaccatt
     1441 tgattacatc gttataacaa tagcttttga caaaatgtat tgtgctatag tatttgcata
     1501 cttaaaatac taacagcaaa ggaatgacag caagatgaaa aaaactctta cagtgacggt
     1561 ttcgtcagtg ttagcttttt tagctttaaa taatgcagca catgcacaac aacatggcac
     1621 acaagtaaaa acacctgttc aacataacta tgtatcaaat gttcaagcac aaacgcaatc
     1681 accgacaact tatacagtag ttgctggcga ttcattatat aagattgctt tagagcatca
     1741 cttaacgttg aatcaattat attcatacaa tcctggtgta acacctttaa tttttcctgg
     1801 tgacgtgatt tcacttgtgc ctcaaaataa agtgaaacaa actaaagcgg ttaaatcacc
     1861 agtaagaaaa gcaagccaag ctaaaaaggt agtaaaacaa cctgtacaac aagcgtctaa
     1921 aaaagtagta gttaagcaag cacctaagca agcagtagct aagacagtta atgtagcata
     1981 taaacctgct caagtacaaa aatcagtacc aactgtacct gttgcacata actacaataa
     2041 atcagttgct aacagaggaa acttatatgc ttatggaaac tgcacatatt atgccttcga
     2101 tcgtcgtgca caattaggta gaagtatagg aagtttatgg ggcaatgcaa ataactggaa
     2161 ttacgcagca aaagttgcag gatttaaagt agataaaaca ccagaagttg gcgctatttt
     2221 ccaaacagct gctggcccat atggacatgt tggtgttgtt gaatctgtaa accctaatgg
     2281 aacaattact gtttcggaaa tgaactatgc tggatttaat gttaaatctt caagaacaat
     2341 tttaaatcca ggaaaatata attacatcca ctaagtaata tatcaagaca agactatcct
     2401 cttagcctgt ttaagtaaca ggttgagagg attttttggt atcatttaat cagagttata
     2461 taaagaagat atttaaatga tatttaattg attgatatgt aaaaagaaag tataatttat
     2521 gattaattaa tggaggaggt aattgaaatg ggtgtacatc aatattttaa aagattatca
     2581 gatatggaaa gacttataag attacctgga aaatttaaat attttgaaca caatgttgca
     2641 gcacactcct ttaaagtaac taaaattgct caatatctag caacagttga agaatatcat
     2701 ggtcgaaaga ttaattggaa aagcttatat gaaaaagcat taaatcatga tttcgctgaa
     2761 gtgtttactg gtgatataaa aacacctgtt aaatatgcga gtagtgaatt aaaaaaatta
     2821 ttttcgcaag ttgaagaaga aatggtagag acctttattg aagaagaaat tccattacaa
     2881 tatagagatg tttataagca acgactgcaa gaaggtaaag atgattcatt agaaggccaa
     2941 atactttcag ttgctgataa aattgatttg ctttatgaaa catttggaga aatacaaaaa
     3001 cgtaatcccg aagaattatt tttcgaaatt tatgaaatga gtctagaaac aattattcaa
     3061 tttgaccatt tagcatctgt acaagatttt attaataata tcattccaga aatgttgact
     3121 gaaaacttta tacctagaac agaattaaga gaaacaacca tgaacatttt aaataaaaga
     3181 aaagaggaaa atgaatgata tggtatttta gcgcagcatt ctttccatgt gtcttggtag
     3241 tattatttag tgtaataaca agaagtaaat gggtcggtac tattctgaca ttaattttaa
     3301 ttggtgcctc aatctataaa gagtatttcc ataacgagtg gattattttt attgatgtag
     3361 tgtcattatt agctggttat ttaattatag atcaactcga atttcataaa catcaagatg
     3421 aagatcgcta agattaactt taaaataatg tttcaaacaa atttgttgaa acaaaacgat
     3481 gattaatata atgtgtattt acatactaaa aataacaaga taaacgattt gatttaaggc
     3541 aagcatagtt agcacaacta tgtttgtttt tctttgttcg acatttttac gaacaaatgt
     3601 ttgctttttg tgtggctact ttgctaaaat atgtaatgag aaagcgaaga gttgagcgga
     3661 aatataaata atacgttgaa agaggaggca tatgtgacaa tggttgaaca ttatcctttt
     3721 aaaatacatt ctgattttga gcctcaaggt gaccaaccgc aagcaattaa ggaaatcgtg
     3781 gatggtatta aagcggggaa aagacatcaa actttattag gtgcaactgg cacagggaaa
     3841 acatttacga tgagtaatgt tattaaagaa gttgggaaac caacgttaat tatcgcacat
     3901 aacaaaacat tagcagggca attatatagt gagtttaaag aattttttcc tgaaaacagg
     3961 gtggaatact ttgtaagtta ctatgattat tatcaacctg aggcatacgt accgtctact
     4021 gacactttta ttgaaaaaga tgcctcaatc aatgatgaaa ttgatcaact acgacattct
     4081 gctacaagtg cattatttga acgcgatgat gtaattatta ttgctagtgt aagttgtata
     4141 tatggtttag gtaatcctga agaatataaa gatttagtag taagtgttcg agttggtatg
     4201 gaaatggata gaagtgaatt acttagaaaa cttgtagatg tgcaatatac acgaaatgac
     4261 atcgatttcc aacgaggaac gtttcgagtg cgtggtgatg tagttgaaat attcccagcc
     4321 tctaaagaag aactttgtat aagggttgag tttttcggcg atgagattga ccgtatccga
     4381 gaagttaact acctaacagg tgaagtgttg aaagaaagag aacattttgc gatattccca
     4441 gcttctcact tcgtaacacg tgaagaaaag ttgaaagttg cgattgaacg tattgaaaaa
     4501 gaattggaag aacgattgaa agaattacga gatgagaata aattactaga agcgcaaagg
     4561 ttagaacagc gtaccaacta tgatttagaa atgatgcgag agatgggatt ctgttcagga
     4621 attgaaaact attccgtaca tttaactttg cgaccactgg gttcgacacc gtatacttta
     4681 ttggattact ttggcgatga ttggttagta atgattgatg aatcacatgt gacattacca
     4741 caagttcgag gcatgtataa cggagacaga gcgcgtaaac aagttttggt ggatcatgga
     4801 tttagattac cgagtgcatt agataaccgt ccacttaaat ttgaagaatt tgaagaaaag
     4861 acaaaacaac ttgtgtatgt atctgcaacg cctggaccat acgaaattga acatacggat
     4921 aagatggttg aacaaattat tcgtcctact ggtttactgg atcctaaaat tgaggttaga
     4981 cctactgaaa atcaaattga cgatttatta agtgaaattc aaacaagggt tgagcgtaat
     5041 gaacgcgtac ttgttacaac gctcactaaa aagatgagtg aagatttaac cacatacatg
     5101 aaagaagcgg gtattaaagt taattatctg cattcagaaa tcaagacatt agaacgaatc
     5161 gaaataatta gagacttacg aatgggtaca tatgatgtta tcgtaggtat taatttatta
     5221 agagagggta ttgatatacc agaagtttct ctagttgtca tattagatgc agataaagaa
     5281 ggatttttac gttctaaccg ctcattaatt caaacaatag gtagagctgc gcgtaacgat
     5341 aaaggtgaag tcattatgta tgccgataaa atgactgatt cgatgaagta tgcaattgat
     5401 gagacacaac gtcgtcgaga aatacagatg aaacataatg aaaaacatgg tattacacct
     5461 aaaacaatta ataaaaaaat acatgattta attagtgcta ctgttgaaaa tgacgaaaat
     5521 aatgacaaag cacaaactgt gatacctaag aagatgacga aaaaagaacg tcaaaagaca
     5581 atcgacaata tagaaaaaga aatgaaacaa gcagcgaaag atttagattt cgagaaagct
     5641 acagaattaa gagatatgtt atttgaatta aaagcagaag ggtgacaagt aaatgaaaga
     5701 accatccata gtagtaaaag gtgctcgtgc gcataacttg aaagatattg atatcgaact
     5761 acctaaaaat aaattaattg ttatgacagg tttatctggg tcaggtaaat cgtcattagc
     5821 attcgatact atatatgctg aaggacaacg acgttatgtt gaatcattaa gtgcctatgc
     5881 gcgtcaattt ttaggccaaa tggacaaacc agatgttgat acaattgaag gattatcgcc
     5941 agcaatttca atagatcaaa aaacaacaag taaaaatcca agatcaactg tagcaacagt
     6001 aacagaaata tatgattata tacgtttgtt atatgcacgt gttggtaaac cttactgtcc
     6061 aaatcacaat atagaaattg aatcgcaaac agtacaacaa atggttgacc gcattatgga
     6121 attagaggca cgtacaaaga ttcaattatt agcacctgtc atcgctcatc gtaaaggtag
     6181 tcatgaaaag ctaatcgaag atattggtaa aaaaggttat gtacgtttaa gaatcgatgg
     6241 cgaaattgtt gatgtaaatg atgtacctac tttagataag aacaagaatc atacaataga
     6301 agttgttgta gaccgattag ttgttaaaga tggaattgaa acacgactag ctgactctat
     6361 agaaactgcg ttagagcttt cagaaggaca attaacagtt gatgtcattg acggggaaga
     6421 ccttaagttt tcagaaagcc atgcttgtcc tatatgtgga ttttcaatcg gagagttaga
     6481 accaagaatg tttagcttta acagtccttt tggtgcttgt ccgacatgtg atggcttagg
     6541 ccaaaagtta acagtcgatg tagacttggt tgttcccgac aaagataaga cgctaaacga
     6601 aggtgcaata gaaccttgga taccgacgag ttctgatttt tatccaacat tgttaaaacg
     6661 tgtttgtgaa gtttataaaa tcaatatgga taaacctttt aaaaagttaa cagaacgtca
     6721 acgtgatatt ttattgtatg gttctggtga caaagaaatt gaatttacat ttacacaacg
     6781 tcaaggtggt actagaaaac gaacaatggt tttcgagggt gtagttccta atataagtag
     6841 acgattccat gaatctcctt cagaatatac acgtgaaatg atgagtaaat atatgaccga
     6901 actaccttgt gaaacttgtc atggaaagcg attgagtcgt gaagcgttat ctgtttatgt
     6961 aggtggttta aatattggtg aagtagtcga atattcaatc agtcaagcgc tgaactatta
     7021 taaaaacatt gatttgtcag aacaagatca agcgattgca aatcaaatat tgaaagaaat
     7081 tatttcccga ctcacttttt taaataatgt gggacttgaa tatttaacgc taaacagagc
     7141 ttcaggtaca ctttcaggtg gtgaagcaca acgtattcga ttggcaacgc aaattgggtc
     7201 gcgtttgact ggtgtcttat atgtattaga tgagccatca attggactgc atcaaagaga
     7261 taatgatcga ttaattaata cacttaaaga aatgagagat ttaggaaata ctttaattgt
     7321 agttgaacac gatgatgata caatgcgtgc ggctgattac ttagtggata tagggcctgg
     7381 tgctggtgaa catggaggac aaattgtgtc tagtgggaca cctcaaaagg taatgaaaga
     7441 taaaaaatca ttaacaggac aatacttgag tggtaagaaa cgtattgacg tacctgaata
     7501 tcgcagaccg gcttcagatc gtaaaatttc tatacgtgga gctagaagca acaatcttaa
     7561 agggattgat gtggacatac cactatcaat catgacggtt gttacaggtg tatcaggttc
     7621 tggtaaaagc tcattagtaa atgaagtatt atacaaatca ttagctcaaa aaattaataa
     7681 atctaaagta aagccaggat tgtacgataa gattgaaggt attgatcaac ttgataaaat
     7741 tattgatatt gatcaatcac cgataggtag aacgccacgc tctaatccag caacatatac
     7801 tggtgtgttt gatgatatac gtgatgtgtt tgcgcaaaca aatgaagcta aaattcgagg
     7861 atatcaaaaa gggcgtttta gttttaatgt aaaaggtgga cgctgtgaag cttgtaaagg
     7921 tgacggtatt attaaaattg aaatgcattt tttacctgat gtttatgttc cttgtgaagt
     7981 gtgtgatggt aaacgatata atcgtgagac actagaggtt acttacaaag gtaaaaatat
     8041 tgctgacatt ttagaaatga ctgttgaaga agcaacacaa ttttttgaaa atattcctaa
     8101 gattaagcgc aagttacaaa cactagttga tgttggtctt ggatacgtca cattaggtca
     8161 acaagctaca acgttatcag gtggtgaggc tcaacgtgtg aaacttgcat ctgaacttca
     8221 taaacgttca actggtaaat ctatttatat cctagacgaa ccaacaacag ggttacatgt
     8281 tgacgatatt agcagattat taaaagtatt aaatcgatta gttgaaaatg gtgacactgt
     8341 tgtaattatt gaacataacc tagatgttat caaaacagca gactatatta tcgacttagg
     8401 tcctgaaggt ggtagtggcg gtggtactat tgttgcgact ggcacacccg aagatattgc
     8461 tcagacaaag tcatcatata caggaaagta tttaaaagaa gtacttgaac gagataaaca
     8521 aaatactgaa gataaataag attaaaagaa gtgaagggtg ttgtaaattt acccttcact
     8581 tctttttatt aatttagtaa tgaatagtag aaagaaaaga tgcgtaaaaa gaattatgtt
     8641 aagatagggt caatctagag ttgctaaaca taaatcgaaa cttagagtgg gacagaaatg
     8701 ataaagaatc actaatgatt tattatgtag tggttctttg tcattagcca cagctattgt
     8761 gtacttaaaa ataggaatgc atgagtgcaa ctcatgcata agaaatacta atttctaaag
     8821 aaaaagtatt tctttatgtt ggggcccacc ccaacttgca ttgtttgtag aatttctttt
     8881 cgaaattctc tgtgttgggg ccccgccaac ttgcattgcc tgtagaattt cttttcgaaa
     8941 ttctttatgt tggggccccg ccaactaatt acaatatatc attgtagagc ttaggtcatt
     9001 gatttatggc tcggactttt atggcgatat gaaccatgta aattaagcaa gcaataaatt
     9061 aatgattgat attgacttgt aaaataataa caataatgaa caattaatat ttattttagc
     9121 ttttcaatgt agattggtgt tatatttttg atatgataag aagagatgta agagtaggga
     9181 taaatacaat tgaggtgaac ccatgttaac gacagaaaaa ctagttgaaa cattaaagtt
     9241 agatttaatc gctggtgaag aaggactatc gaagccaatt aaaaatgctg atatatcaag
     9301 accaggctta gagatggcag gttatttttc acattatgcg tcagatagaa tacaactatt
     9361 aggaacaacg gaactatcgt tttacaattt attaccagat aaggatcgcg caggtcgtat
     9421 gcgtaaacta tgcagaccag aaacgcctgc aattattgtg acacgtggat tgcagccacc
     9481 agaagaatta gttgaagctg caaaagaatt aaatacccca cttatagttg ctaaagatgc
     9541 gactacaagt ttaatgagtc gcttaacaac gtttttagag catgcacttg caaagacgac
     9601 atctttacat ggtgttttag tagatgttta cggtgttggt gtactaatta ccggtgattc
     9661 aggaataggt aaaagtgaga ctgcgttgga attagttaaa cgtgggcata gattagtagc
     9721 agatgataat gtagaaatac gtcaaattaa taaagatgaa ctaataggga aaccaccaaa
     9781 gttaatagaa catctattag aaatacgtgg actaggtatt atcaatgtta tgactttatt
     9841 tggcgcgggt tcaatattaa ctgaaaaacg aattagatta aatattaatt tggaaaactg
     9901 gaacaagcaa aagttatatg accgcgtagg tcttaatgaa gagacgctaa gtattttaga
     9961 tactgaaatc actaaaaaaa caatacctgt aagacctggt agaaatgttg cggtaattat
    10021 tgaggtcgct gcaatgaact atcgattaaa tatcatgggc attaacactg ccgaagaatt
    10081 tagtgaaaga ttaaatgaag aaattatcaa gaacagtcat aagagtgagg agtaggttga
    10141 atgggtattg tatttaacta tatagatcct gtggcattta acttaggacc actgagtgta
    10201 cgatggtatg gaattatcat tgctgtcgga atattacttg gttactttgt tgcacaacgt
    10261 gcactagtta aagcaggatt acataaagat actttagtag atattatttt ttatagtgca
    10321 ctatttggat ttatcgcggc acgaatctat tttgtgattt tccaatggcc atattacgcg
    10381 gaaaatccaa gtgaaattat taaaatatgg catggtggaa tagcaataca tggtggttta
    10441 ataggtggct ttattgctgg tgttattgta tgtaaagtga aaaatttaaa cccatttcaa
    10501 attggtgata tcgttgcgcc aagtataatt ttagcgcaag gaattggacg ctggggtaac
    10561 tttatgaatc acgaggcaca tggtggaccg gtgtcacgcg cttttttaga acaattacat
    10621 ttgcctaatt ttataataga aaatatgtat attaacggcc aatattatca tccaacattc
    10681 ttatatgaat ccatttggga tgtcgctgga tttattatct tagttaatat tcgtaaacat
    10741 ttaaaattag gagaaacatt ctttttatat ttaacttggt attcaattgg tcgattcttt
    10801 atagaaggat tacgtacaga tagcttaatg ctcacaagta atattagagt tgcacaatta
    10861 gtatcaattc ttttaatttt aataagtata agtttaattg tatatagaag gattaagtat
    10921 aatccaccgt tgtatagcaa agttggggcg cttccatggc caacaaaaaa agtgaagtag
    10981 tgatattttg agaaaatttt tatcaaaaac acatcatcat acaaaccctt tatggcgtgt
    11041 ataccgtctt gttaaatttt cgaaagtttt taagaatgta attatcattg aattttcgaa
    11101 atttattcca agtatggtac tgaaaagaca tatatataaa caacttttaa atattaatat
    11161 cggtaatcaa tcatcgatag cttataaagt aatgttagat attttttacc cagaactgat
    11221 tacgattggt agtaacagtg ttattggtta caatgtaaca attttgacgc atgaagcatt
    11281 agttgatgaa tttcgttatg gaccagtgac gataggatct aacactttga ttggtgcaaa
    11341 tgctaccatt ttacccggta taacgattgg tgacaatgta aaagttgcag ctggtacggt
    11401 tgtttcaaaa gatataccgg ataatggatt tgcatatggc aaccctatgt atataaaaat
    11461 gattaggagg tgacaatttt atggcgcaaa agaataataa tgtaattcca atgacttttg
    11521 atgatgcatt ttatcgtaaa atggctaaac agaagtttaa acaaagagaa tataaacgag
    11581 ctgctgaata ctttgaaaaa gtgttagaat tgtcacctga tgatctggaa attcaaattg
    11641 attatgcaca atgtctagtg caacttggta ttgctaaaaa agcagaacat ttattttatg
    11701 acaatattat ttataatagg catctagaag atagctttta tgaattgagt cagctcaaca
    11761 ttgaagttaa cgaaccaaac aaggcattct tgtttggtat taattatgtt attgttagcg
    11821 acgaccaaga ttatagagat gaattagatc aaatgtttga tgtgaaatat caaagcgaag
    11881 aacaaattga acttgaagct caattgtttg tagttcaaat actattccaa tatctttttt
    11941 ctcaaggtcg attaaaagat gcaaagaatt atgtcttaca tcaaccacaa gaagttcaag
    12001 atcatcgtgt cgtacgtaat ttattggcaa tgtgttattt atatctcggt gaatatgata
    12061 cggctaaagc attgtacgaa gcactattac aagaggatag tacagatata tatgcattat
    12121 gccattatac tttgctactt tataacacta aggaaaatga acaatatcaa aaatatttaa
    12181 aaatattaaa caaagttgta cctatgaatg acgatgaaag ttttaaatta ggtattgtat
    12241 taagttattt aaagcagtat cgtgcatcac aacaattgtt gtacccttta tataaaaaag
    12301 ggaaattttt atcaattcaa atgtacaatg ctttagcata taattattat tatttaggtg
    12361 aagaagacga aagtcattac tactgggata aattgaagca aatttctaaa gtggaaattg
    12421 gacatgcgcc ttgggtaatt gaaaatagca aagaagtttt tgaccaacat attttgccat
    12481 tacttcaaag tgatgacagt cattatcgtt tatatggtat ttttttattg gatcaattaa
    12541 atggtaaaga aattgtgatg acggaaagta tttggcaggt tctggaaaat ctaaataatt
    12601 atgagaaatt gtatttaacg tatttagttc aaggtttaac gctcaataaa ttagacttca
    12661 ttcatcgcgg cttgttaacg ctttaccaaa atgaattatt tgtaagtgaa aatgatttaa
    12721 tggttgcatg gattaatcaa ggtgaactca taattgctga aaaagtagat ttaactgatg
    12781 ttgagccata tatcggtgcg tttatttatt tgtattttaa aaatcaacct cgaaacgtta
    12841 caaagaagca aattacaaca tggttaggca taacacaata taaactgaac aaaatgattg
    12901 aatttctctt gagcatatag atttatgaaa agttagattt attatataat gcgcataatg
    12961 attaataatg aggaggcgtt aataaaatga ctgaaataga ttttgatata gcaattatcg
    13021 gtgcaggtcc agctggtatg actgctgcag tatacgcatc acgtgctaat ttaaaaacag
    13081 ttatgattga aagaggcatt ccaggcggtc aaatggctaa tacagaagaa gtagagaact
    13141 tccctggttt cgaaatgatt acaggtccag atttatctac aaaaatgttt gaacacgcta
    13201 aaaagtttgg tgcagtttat caatatggag atattaaatc tgtagaagat aaaggcgaat
    13261 ataaagtgat taactttggt aataaagaat taacagcgaa agcggtcatt attgctacag
    13321 gtgcagaata caagaaaatt ggtgttccgg gtgaacaaga acttggtgga cgcggtgtaa
    13381 gttattgtgc agtatgtgat ggtgcattct ttaaaaataa acgcctattc gttatcggtg
    13441 gtggtgactc agcagtagaa gagggaacat tcttaactaa atttgctgac aaagtaacaa
    13501 tcgttcaccg tcgtgatgag ttacgtgcac agcgtatttt acaagataga gcattcaaaa
    13561 atgataaaat cgactttatt tggagtcata ctttgaaatc aattaatgaa aaagacggca
    13621 aagtgggttc tgtgacatta acgtctacaa aagatggttc agaagaaaca cacgaggctg
    13681 atggtgtatt catctatatt ggtatgaaac cattaacagc gccatttaaa gacttaggta
    13741 ttacgaatga tgttggttat attgtgacaa aagatgatat gacaacatca gtaccaggta
    13801 tttttgcagc aggagatgtt cgtgacaaag gtttacgcca aattgtcact gctactggcg
    13861 atggtagtat tgcagcgcaa agtgcagcgg aatatattga acatttaaac gatcaagctt
    13921 aattcgaggt cgaattaaga tgttgagttg taagttattt ggatatttat tttaatagtg
    13981 tcatcacctt gttaaaataa tgtcttactt ttaaattaaa gcaaattata tagaaaacta
    14041 gaacttagta cgtatcattt gtgcgtttca atgagttcta gtttttttat atgttatatt
    14101 aaacttataa ctttatggga gtgggacaga aatgataaag agccactaat gatttattat
    14161 gtagtggttc ttatacatta gccacagcta atgtgtactt aaaaatagga atacatgagt
    14221 aaaactcatg cataagaaat actaatttct atagaaaaag tattacttta tcgttgtacc
    14281 accccaactt gcacattatc gtaagctgac ttatcgtaag cttctgtgtt ggggcccaca
    14341 ccccaactcg cattgcctgt agaatttctt ttcgaaattc tctttgttgg ggcccacacc
    14401 ccaacttgca ttgtctgtag aaattggaaa tccaatttct ctgtgttggg gcccacaccc
    14461 caactcgcat tgcctgtaga atttcttttc gaaattctct gtgttggggc ccacacccca
    14521 acttgcattg tctgtagaaa ttggaaatcc aatttctctg tgttggggcc cctgactaga
    14581 gttgaaaaaa gcttgttgca agcgcatttt cattcagtca actactagca atataatatt
    14641 atagacccta ggacattgat ttatgtccca agctccttta aatgatgtat atttttagaa
    14701 atttaatcta gacatagttg gaaataaata taaaacatcg ttgcttaatt ttgtcataga
    14761 acatttaaat taacatcatg aaattcgttt tggcggtgaa aaaataatgg ataataatga
    14821 aaaagaaaaa agtaaaagtg aactattagt tgtaacaggt ttatctggag caggtaaatc
    14881 tttggttatt caatgtttag aagacatggg atatttttgt gtagataatc taccaccagt
    14941 gttattgcct aaatttgtag agttgatgga acaaggaaat ccatccttaa gaaaagtggc
    15001 aattgcaatt gatttaagag gtaaggaact atttaattca ttagttgcag tagtggataa
    15061 agttaaaagt gaaagtgacg tcatcattga tgttatgttt ttagaagcaa gtactgaaaa
    15121 attaatttca agatataaag aaacgcgtcg tgcacatcct ttgatggaac aaggtaaaag
    15181 atccttaatt aatgcaatta atgatgagcg agagcatttg tctcaaatta gaagtatagc
    15241 taattttgtt atagatacta caaagttatc acctaaagaa ttaaaagaac gcattcgtcg
    15301 atactatgaa gatgaagagt ttgaaacttt tacaattaat gtcacaagtt tcggttttaa
    15361 acatggaatt cagatggatg cagatttagt atttgatgta cgatttttac caaatccata
    15421 ttatgtagta gatttaagac ctttaacagg attagataaa gacgtttata attatgttat
    15481 gaaatggaaa gagacggaga ttttctttga aaaattaact gatttgttag attttatgat
    15541 acccgggtat aaaaaagaag ggaaatctca attagtaatt gccatcggtt gtacgggtgg
    15601 acaacatcga tctgtagcat tagcagaacg actaggtaat tatctaaatg aagtatttga
    15661 atataatgtt tatgtgcatc atagggacgc acatattgaa agtggcgaga aaaaatgaga
    15721 caaataaaag ttgtacttat cggtggtggc actggcttat cagttatggc taggggatta
    15781 agagaattcc caattgatat tacggcgatt gtaacagttg ctgataatgg tgggagtaca
    15841 gggaaaatca gagatgaaat ggatatacca gcaccaggag acatcagaaa tgtgattgca
    15901 gctttaagtg attctgagtc agttttaagc caactttttc agtatcgctt tgaagaaaat
    15961 caaattagcg gtcactcatt aggtaattta ttaatcgcag gtatgactaa tattacgaat
    16021 gatttcggac atgccattaa agcattaagt aaaattttaa atattaaagg tagagtcatt
    16081 ccatctacaa atacaagtgt gcaattaaat gctgttatgg aagatggaga aattgttttt
    16141 ggagaaacaa atattcctaa aaaacataaa aaaattgatc gtgtgttttt agaacctaac
    16201 gatgtgcaac caatggaaga agcaatcgat gctttaaggg aagcagattt aatcgttctt
    16261 ggaccagggt cattatatac gagcgttatt tctaacttat gtgtcaatgg tatttcagat
    16321 gcgttaattc attctgatgc gcctaagcta tatgtttcta atgtgatgac gcaacctggg
    16381 gaaacagatg gttatagcgt gaaagatcat atcgatgcga ttcatagaca agctggacaa
    16441 ccgtttattg attatgtcat ttgtagtaca caaactttca atgctcaagt tttgaaaaaa
    16501 tatgaagaaa aacattctaa accagttgaa gttaataagg ctgaacttga aaaagaaagc
    16561 ataaatgtaa aaacatcttc aaatttagtt gaaatttctg aaaatcattt agtaagacat
    16621 aatactaaag tgttatcgac aatgatttat gatatagctt tagaattaat tagtactatt
    16681 cctttcgtac caagtgataa acgtaaataa tatagaacgt aatcatatta tgatatgata
    16741 atagagctgt gtaaaaaaat gaaaatagac agtggttcta aggtgaatca tgttttaaat
    16801 aagaaaggaa tgactgtacg atgagctttg catcagaaat gaaaaatgaa ttaactagaa
    16861 tagacgtcga tgaaatgaat gcaaaagcag agctcagtgc gctgattcga atgaatggtg
    16921 cacttagtct ttcaaatcaa caatttgtta taaatgttca aacggaaaat gcaacaacgg
    16981 caagacgtat ttattcgttg attaaacgtg tctttaatgt ggaagttgaa atattagtcc
    17041 gtaaaaaaat gaagcttaaa aaaaataata tttatatttg tcgtacaaag atgaaagcga
    17101 aagaaattct tgatgaatta ggaattttaa aagacggcat ttttacgcat gaaattgatc
    17161 attcaatgat tcaagatgat gaaatgagac gcagttactt gagaggagct tttctggcag
    17221 gtggctcagt gaataaccct gaaacatctt cgtaccattt ggaaattttt tctcaaaatg
    17281 agagtcatgc agaaggctta acgaaactaa tgaatagtta tgagttgaat gccaaacatt
    17341 tagagcgaaa aaaaggaagt attacgtatt taaaagaagc ggaaaagatt tcggattttc
    17401 ttagtttgat aggtggctat caagcgttat taaaatttga agacgtacgt attgtaagag
    17461 atatgcgtaa ttctgttaac cgactcgtta attgtgaaac ggcaaatcta aataaaacag
    17521 ttagtgctgc gatgaaacaa gttgagagca ttaaattgat tgataaagaa attggtattg
    17581 aaaatttacc agacaggttg agagagattg ctagaattcg agtagaacat caagaaattt
    17641 cgttgaaaga gcttggagaa atggtatcaa ctggtccaat ttcaaaatca ggtgtaaatc
    17701 accgattaag aaaacttaat gatttagccg ataagattag aaatggtgaa caaatagaat
    17761 tataagtaag aaggtgtttt tggtagtgaa ttatcaaaaa tacctttttt atgattaaaa
    17821 agtgtttaat gaataatagt cgactaacta ttaaattgag ctggaaagtt taatgaagga
    17881 aattcaataa ccaagcgtta cctaattttc tcattaagta gcatcatatg tatcaatttg
    17941 gtcataagaa aatatgaatg tgataattaa ttgagtcact gatcatagac aaaaacaaaa
    18001 tcagcataaa taagaatccc gactttgcgg gattctctgt attgaaaagt attgtatttt
    18061 attaaaacag cctccttgaa gggaattgaa cccctatctt aagaaccgga atcttacgtg
    18121 ttatccatta caccacaagg agcaaatatg taatcatatc tctgtactat gagaagtaaa
    18181 ataagtacaa tctaaaatag atattatcag tttacaaagg aaagagaaaa gcgtcaaaca
    18241 atgtaactat ttaaagtcaa agtgtttgac caaatttgac ttaatatgta aaataatgag
    18301 taacagttat tacaaggagg aaatatagat gaatttaatt cctacagtta ttgaaacaac
    18361 aaaccgcggt gaacgtgcat atgatatata ctcacgttta ttaaaagacc gtattattat
    18421 gttaggttca caaattgatg acaacgtagc aaattcaatc gtatcacagt tattattctt
    18481 acaagcgcaa gactcagaga aagatattta tttatacatt aattcaccag gtggaagtgt
    18541 aacagctggt tttgcgattt atgatacaat tcaacacatt aaacctgatg ttcaaacaat
    18601 ttgtatcggt atggctgcat caatgggatc attcttatta gcagctggtg caaaaggtaa
    18661 acgtttcgcg ttaccaaatg cagaagtaat gattcaccaa ccattaggtg gtgctcaagg
    18721 acaagcaact gaaatcgaaa ttgctgcaaa tcacatttta aaaacacgtg aaaaattaaa
    18781 ccgcatttta tcagagcgta ctggtcaaag tattgaaaaa atacaaaaag acacagatcg
    18841 tgataacttc ttaactgcag aagaagctaa agaatatggc ttaattgatg aagtgatggt
    18901 acctgaaaca aaataattca aagtaaagag tagactaagc agtctgctct ttttgtatga
    18961 gtaaaccgag gtgtcaataa attgtttact atactttgag cggaaatatg attgaatgaa
    19021 gctagttgaa ccgtaactat atgaaatgtt cccttcaaag tagacattga aaggaacatt
    19081 tcaatccttt gtttgtaagt cgctctagac attacattta gtacatatgt tgtttctaat
    19141 gctcattaat ggtattgatt attctttgat taaatcttca agtgccattt ttaaattact
    19201 atatttaaat tggaatccca atgcttgaat tttattaggt aatacttttt gagtatccaa
    19261 tactactgtt gacatttgac caagtatgag acgcattgca agacttggtg cccaagtttc
    19321 atggggctta tgcatagctc ttgctaaagt gtagccaaat aaattttgac gctcaggtat
    19381 aggtgcagtt aaattaaacg gaccactagc tgactcgtta tttattaaaa ataaaatagc
    19441 ttgaattaaa tcattgatat gaatccatga ataccattgt tgaccagaac ctaatttacc
    19501 accaatgtaa tatttgtatg gtagtttcat tgtttgtaac gcaccgcctt cattcgataa
    19561 aatcataccg aaacgaccga tgacaactcg cgtacctaat tgttcaaatt gttgtgcgaa
    19621 acgttcccat tgatacacaa tatctgataa gaaatcaaat ggtaaagttt tataaacttc
    19681 tgtgtaactc ataaataaat caggaggata gtaaccagtg gcgctagcat taaataaaac
    19741 tttaggtgct ttattacgtg atttaaacaa ttcatataaa gcttgcgtag attgaattct
    19801 acttagcatt agcgtttgtt tatattccgg tgtccatcgt ttattcagtg tagcacctgc
    19861 taagttgatg accacatcga tattttgagg aactttgtgt tcccacccag atttagccca
    19921 gttgacatat gaaattttct tatcatttga aatttggtcg tgtcgcgtta atatcgtgat
    19981 atgtgaatct gattttttaa tttcattaac taattgagat ccaaccatac cagtcccacc
    20041 agtaattaag tattgtttca ttatcattca ccccatgtaa ttttgtattt agttactatt
    20101 t
//
LOCUS       DAHHSC010000027        19679 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.027, whole genome shotgun sequence.
ACCESSION   DAHHSC010000027 DAHHSC010000000
VERSION     DAHHSC010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 19679)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19679)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19679
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.027"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            164..1690
                     /gene="spa"
                     /locus_tag="NBI57_000763"
     CDS             164..1690
                     /gene="spa"
                     /locus_tag="NBI57_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphylococcal protein A"
                     /protein_id="HCD1821729.1"
                     /translation="MKKKNIYSIRKLGVGIASVTLGTLLISGGVTPAANAAQHDEAQQ
                     NAFYQVLNMPNLNADQRNGFIQSLKDDPSQSANVLGEAQKLNDSQAPKADAQQNNFNK
                     DQQSAFYEILNMPNLNEAQRNGFIQSLKDDPSQSTNVLGEAKKLNESQAPKADNNFNK
                     EQQNAFYEILNMSNLNEEQRNGFIQSLKDDPSQSANLLSEAKKLNESQAPKADNKFNK
                     EQQNAFYEILHLPNLNEEQRNGFIQSLKDDPSQSANLLAEAKKLNDAQAPKADNKFNK
                     EQQNAFYEILHLPNLTEEQRNGFIQSLKDDPSVSKEILAEAKKLNDAQAPKEEDNKKT
                     GKEDGNKPGKEDGNKPGKEDNKKPGKEDGNKPGKEDNNKPGKEDGNKPGKEDNNKPGK
                     EDGNKPGKEDGNKPGKEDGNGVHVVKPGDTVNDIAKANGTTADKIAADNKLADKNMIK
                     PGQELVVDKKQPANHADANKAQALPETGEENPFIGTTVFGGLSLALGAALLAGRRREL
                     "
     gene            complement(2019..3611)
                     /locus_tag="NBI57_000764"
     CDS             complement(2019..3611)
                     /locus_tag="NBI57_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HCD1821730.1"
                     /translation="MTLLTVNPFDNVGLSALVAAVPIILFLLCLTVFKMKGIYAALTT
                     LVVTLIVALFVFELPARVSAGAITEGVVAGIFPIGYIVLMAVWLYKVSIKTGQFSIIQ
                     DSIASISEDQRIQLLLIGFCFNAFLEGAAGFGVPIAICAVLLIQLGFEPLKAAMLCLI
                     ANGAAGAFGAIGLPVSIIDTFNLSGGVTTLDVARYSALTLPILNFIIPFVLVFIVDGM
                     KGIKEILPVILTVSGTYTGLQLLLTIFHGPELADIIPSLATMVVLAFVCRKFKPKNIF
                     RLEASEHKIQKRTPKEIVFAWSPFVILTAFVLVWSAPFFKKLFQPGGALESLVIKLPI
                     PNTVSDLSPKGIALRLDLIGATGTAILLTVIITILITKLKWKSAGALLVEAIKELWLP
                     ILTISAILAIAKVMTYGGLTVAIGQGIAKAGAIFPLFSPVLGWIGVFMTGSVVNNNTL
                     FAPIQATVAQQISTSGSLLVAANTAGGVAAKLISPQSIAIATAAVKKVGEESALLKMT
                     LKYSIIFVAFICVWTFILTLIF"
     gene            complement(3873..4346)
                     /locus_tag="NBI57_000765"
     CDS             complement(3873..4346)
                     /locus_tag="NBI57_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1648 domain-containing protein"
                     /protein_id="HCD1821731.1"
                     /translation="MSKIRSFTILSLLIYLAMMCYTVVTYSKLPTKVPIHYNLAGDAD
                     NFADKWVLLLINSAFIVIWLIFFIAGRYYERFAKWSHYNHTPREIRAIKLFLSTLNLE
                     IMSYMSIFTVLEIWQIQHHHQLNLLWFNMIFIIIIGLTLVIFCLLPTIHKMRDSQ"
     gene            complement(4536..5933)
                     /locus_tag="NBI57_000766"
                     /pseudo
     CDS             complement(4536..5933)
                     /locus_tag="NBI57_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498667.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
     gene            6046..6918
                     /locus_tag="NBI57_000767"
     CDS             6046..6918
                     /locus_tag="NBI57_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HCD1821732.1"
                     /translation="MNKLRDTTFLSYLFTIILWGSAFPMIKIALNDFSAESLSAFRLI
                     LATIILLPFVIIKKLPTPELRDIPVIFILGFCGFVIYHTALNFGETLISAGISGILVS
                     TTPIFSSALAYIFLKEHFSKWNWLSSLVAFIGISIISISKDDYTTINVLGVFIILLAS
                     FSESLYFTFQKKYIEKYGFIAFTLYTIMASSPFMLIFIPEIINDIHGATFTSIVSVLY
                     LAIFPTIIPYVLLAYIVKSVGVSDATMSLYLTPIVSLLLSYLLLDELPTTLAIIGGII
                     TLLGVSLSNFFQNT"
     gene            7092..8867
                     /locus_tag="NBI57_000768"
     CDS             7092..8867
                     /locus_tag="NBI57_000768"
                     /EC_number="4.2.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oleate hydratase"
                     /protein_id="HCD1821733.1"
                     /translation="MYYSYGNYEAFARPKKPENVENKSAYLIGSGLASLAAACFLIRD
                     GQMEGSKIHILEELPKAGGSLDGENMPLKGYVVRGGREMENHFECLWDLFRSIPSLEI
                     DNASVLDEFYWLNKEDPNYSRCRVIEKQGQRLVTDGDFTLTKTAIKEILDLCLTNEED
                     LDDVKITDVFSDDFFNSNFWIYWKTMFAFEPWHSAMEMRRYLMRFVHHIGGLADFSAL
                     KFTKYNQYESLVLPMVEYLKSHGVQFEYDVKVEDIKVDVTTSQKIAREILIHRHGKAE
                     SIKLTVDDLVFVTNGSITESSTYGDNDTPAPPTDELGGSWTLWKNLARQSPEFGNPDK
                     FCQNIPKKSWFVSATSTTNNKEIIDTIESICKRDPLAGKTVTGGIITINDSAWQMSFT
                     INRQQQFKDQPENEISTWIYALYSDVNGDYIKKPITECSGNEICQEWLYHLGVSTDKI
                     EDLAKHVSNTIPVYMPYITSYFMTRAIGDRPLVVPHQSQNLSFIGNFAETERDTVFTT
                     EYSVRTAMEAVYQLLNIDRGIPEVINSTFDLRVLMDAVYELNDHQDLREITKDSKMQK
                     LALAGFLKKIKGTYIETLLKEHKLL"
     gene            9192..10853
                     /locus_tag="NBI57_000769"
     CDS             9192..10853
                     /locus_tag="NBI57_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HCD1821734.1"
                     /translation="MSVTEVIFSFLGGLGIFLYGLKIMGDGLQASAGDRLRDILNKFT
                     SNPVLGVIAGIVVTILIQSSSGTTVITIGLVTAGFMTLKQAIGVIMGANIGTTVTAFI
                     IGIDLGEYAMPILALGAFLIFFFKRSKINNIGRILFGFGSLFFGLEFMGDAVKPLASL
                     DGFKQLMLDMSSNPILAVIVGAGLTALVQSSSATIGILQEFYQQDLISLNAAIPVLLG
                     DNIGTTITAILASLAGSIAAKRAALVHVIFNLIGVIIFTIFLPVVIHLISLLQDVWHL
                     KPAMTIAVSHGTFNITNTLIQLPFVAGLAWIVTKLVPGKDIADDYKPQHLNKDLVYHA
                     PGVALQETQKELQNVGQIVLSMFEDIREITKDDKKLIKKLEQKHQAVETINDSIRNYL
                     VRISTKAITKADVERLAVMFDVNRSILKVAELTEEYVAQLKRQHDEDIRITEDAQRGM
                     DKLFNHVAESFDKAIDMLDVYDKTKKDEIVERSRESFNIEHKLRKGHIKRLNRGECTT
                     KGGLLYIDMIGVLERIGYHSRNVSEALVGLNDDVPTDEEIATTEI"
     gene            complement(11337..12725)
                     /locus_tag="NBI57_000770"
     CDS             complement(11337..12725)
                     /locus_tag="NBI57_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCD1821735.1"
                     /translation="MNETYRGGNKLILGIVLGVITFWLFAQSLVNVVPNLQQSFGADM
                     GTISIAVSLTALFSGMFVVGAGGLADKIGRVKMTNIGLLLSIIGSALIIITNLPALLI
                     LGRVIQGVSAACIMPSTLAIMKTYYQGAERQRALSYWSIGSWGGSGICSLFGGAVATT
                     MGWRWIFIFSIIVAVLSMLLIKGTPETKSEITNTHKFDVAGLIVLVVMLLSLNVVITK
                     GAALGYTSLWFFGLIAIVIVAFFIFLNVEKKVDNPLIDFKLFENKPYTGATISNFLLN
                     GVAGTLIVANTFVQQGLGYTALQAGYLSITYLIMVLLMIRVGEKLLQKMGSKRPMLLG
                     TFIVVIGIALISLVFLPGIFYVISCVVGYLCFGLGLGIYATPSTDTAISNAPLDKVGV
                     ASGIYKMASSLGGAFGVAISGAVYVGAVAATSIHTGAMIALWVNVLMGIMAFIAILFA
                     IPNDDKRVKDAK"
     gene            complement(12727..13905)
                     /locus_tag="NBI57_000771"
     CDS             complement(12727..13905)
                     /locus_tag="NBI57_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HCD1821736.1"
                     /translation="MNQQLIEALKSKEDKMIEIRRYLHQHPELSFHEDETAKYIAEFY
                     KGKDVEVETNVGPRGIKVTIDSGKPGKTLAIRADFDALPITEDTGLSFASQNKGVMHA
                     CGHDAHTAYMLVLAETLAEMKDSFTGKVVVIHQPAEEVPPGGAKAMIENGVLDGVDHV
                     LGVHVMSTMKTGNVYYRPGYVQTGRAFFKLKVQGKGGHGSSPHMANDAIVAGSYFVTA
                     LQTVVSRRLSPFETGVVTIGSFDGKGQFNVIKDVVEIEGDVRGLTDATKATIEKEIKR
                     LSKGLEDMYGVTCTLEYNDDYPALYNDPEFTEYVAKTLKEANLDFGVEMCEPQPPSED
                     FAYYAKERPSAFIYTGAAVEDGEIYPHHHPKFNISEKSLLISAEAVGTVVLDYLKGDN
                     "
     gene            complement(14056..16293)
                     /locus_tag="NBI57_000772"
     CDS             complement(14056..16293)
                     /locus_tag="NBI57_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020808042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCD1821737.1"
                     /translation="MQRDYLIRVETESMPDFKRLNGLMIGFVIKGEAHIYDENNMTQC
                     NSGDIFIINHRDLYRFQLQQDGIICYIQFQMKYLADKFDDAHCLYFHLTDATTTKNIH
                     QLRNIMARLVSTHIRHNELSKLTEQQLVIQLLMHMIHYVPRTYHSNQSILNDDKVNQV
                     CDYIELHFHEDLSLSELSEYVGWSESHLSKKFTESLGVGFQHFLNTTRIEHAKLDLTY
                     TDETITDIALQNGFSSAASFARTFKHFTHQTPKQYRGDRPAITENQQSAQHNYHDREL
                     ILLLNDYIEEMNHFIEDIEKMNYKEIAFKPTNQQLNQFNHIIQVGYLRNLLNTQYQSQ
                     LLTCHHDFQVNEVLAYDVMPYIMKKLNAPFTYDAEISNIFYDIDLCLDFLLDHNFSLT
                     MHLNQYDSRDYIDAFKVFIHHVALHVSHRKDLKFNLYVTTLHTSLIEMIDYFKALFPN
                     GGLYIHLDQATERHLPLLKRLEPHINHFVFDANSNDAVDFNKMNDDEFKTASQMIINK
                     TNYLIDLMHRHNLKRPLILLNWNTLTGDTFITNGEYFRGGIIIEQLLKLSTKVEGIGY
                     WLNYDLHVSHCRNERDYMNSIELFHQYNGKRPVYFTALLFNKLTSNILYSDDTCIVTG
                     TDSNFQILLYDAKHFNPYLALDNQMNMRATEMIHLNINALEDGMYKIKHFTLDKENGA
                     LFNLWRKHHTIHGMDKDSIDYVNRMSFPKLEVYDIDITDTLALNIKMITNGIHLIEVK
                     RYPSS"
     gene            complement(16390..17160)
                     /locus_tag="NBI57_000773"
     CDS             complement(16390..17160)
                     /locus_tag="NBI57_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein"
                     /protein_id="HCD1821738.1"
                     /translation="MMKRLNKLVLGISFLFLVISFTAGCGMGKEAEIKKSFEKTLSMY
                     PIKNLEDLYDKEGYRDDQFDKNDKGTWIINSEMVVQPKGERMKSKGMVLYMNRNTKTT
                     TGKYIVSETLHDEDGRPKSKDKEYPVKMVDNKIIPTKGIKDENIKKEIENFKFFAQYG
                     SFKDLSKYKDGDISYNPEVPSYSAKYQLTNDDYNVKQLRKRYKIPTNKAPKLLLKGSG
                     DLKGSSVGYKDIEFTFVEKKGENTFFTDSLHLEPSEDK"
     gene            complement(17225..17995)
                     /locus_tag="NBI57_000774"
     CDS             complement(17225..17995)
                     /locus_tag="NBI57_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein"
                     /protein_id="HCD1821739.1"
                     /translation="MMKRLNTLVLYISFLILIISIVAGCGTGKEAEIKKSFEKTLSMY
                     PIKNLEDLYDKEGYRDDEFDKNDKGTWTISSEMAIQKKGEALNIKGMVLKLNRNTRSA
                     KGFYYVNAIKKDENGRPQDKQIEYPVKMIDNKIIPTKDIKDEKIKKEIENFKFFAQYG
                     NFKDLTKYKGGDISYNPEAPIYSAKYQLTNDDYNVKQLRKRYDIPTNKAPKLLLKGSG
                     NLDGSSIGYKKIEFTFVEKKGENTYFTANLHFKPSNDE"
     gene            complement(18060..18830)
                     /locus_tag="NBI57_000775"
     CDS             complement(18060..18830)
                     /locus_tag="NBI57_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein"
                     /protein_id="HCD1821740.1"
                     /translation="MIKRVNKLVLGISFLFLIISIFAGCGTGKEAEIKKSFEKTLSMY
                     PIKNLEDLYDKEGYRDDQFDKNDKGTWIVRSSMSIQSNGKDMNIKGMVLYMNRNTRTT
                     NGYYYVDVIEREDKGIHRDNEKRYPVKMVDNKIIPTKEIKDEKIKKEIENFKFFVQYG
                     DFKDLSKYKDGDISYNPEVPSYSAKYQLTNDDYNVKQLRKRYNIPTNKAPKLLLKGTG
                     NLKGSSIGYKKIEFTFVEKKGENIYFSDGLHFNPSEDK"
     gene            complement(18882..19652)
                     /locus_tag="NBI57_000776"
     CDS             complement(18882..19652)
                     /locus_tag="NBI57_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein"
                     /protein_id="HCD1821741.1"
                     /translation="MMKRLNKLVLGIIFLFLVISITAGCGIGKEAEVKKSFEKTLSMY
                     PIKNLEDLYDKEGYRDDQFDKNDKGTWIINSEMVIQPNNEDMVAKGMVLYMNRNTKTT
                     NGYYYVDVTKDEDEGKPHDNEKRYPVKMVDNKIIPTKEIKDKNIKKEIENFKFFVQYG
                     NFKDLSKYKDGDISYNPEVPSYSAKYQVTNDDYNVKQLRKRYDIPTNKAPKLLLKGTG
                     NLKGSSVGYKDIEFTFVEKKGENIYFSDSLHLEPSEDK"
BASE COUNT         6469 a         3577 c         2927 g         6706 t
ORIGIN      
        1 ggttttaagc cttttacttc ctgaataaat ctttcagaaa aatatttatt ttataagttg
       61 taaaacttac atttaaattt aattataaat atagatttta gtattgcaat acataattcg
      121 ttatattatg atgactttac aaatacatac agggggtatt aatttgaaaa agaaaaacat
      181 ttattcaatt cgtaaactag gtgtaggtat tgcatctgta actttaggta cattacttat
      241 atctggtggc gtaacacctg ctgcaaatgc tgcgcaacac gatgaagctc aacaaaatgc
      301 tttttatcaa gtgttaaata tgcctaactt aaacgctgat caacgtaatg gttttatcca
      361 aagccttaaa gatgatccaa gccaaagtgc taacgtttta ggtgaagctc aaaaacttaa
      421 tgactctcaa gctccaaaag ctgatgcgca acaaaataac ttcaacaaag atcaacaaag
      481 cgccttctat gaaatcttga acatgcctaa cttaaacgaa gcgcaacgta acggcttcat
      541 tcaaagtctt aaagacgacc caagccaaag cactaatgtt ttaggtgaag ctaaaaaatt
      601 aaacgaatct caagcaccga aagctgataa caatttcaac aaagaacaac aaaatgcttt
      661 ctatgaaatc ttgaatatgt ctaacttaaa cgaagaacaa cgcaatggtt tcatccaaag
      721 cttaaaagat gacccaagcc aaagtgctaa cctattgtca gaagctaaaa agttaaatga
      781 atctcaagca ccgaaagcgg ataacaaatt caacaaagaa caacaaaatg ctttctatga
      841 aatcttacat ttacctaact taaacgaaga acaacgcaat ggtttcatcc aaagcttaaa
      901 agatgaccca agccaaagcg ctaacctttt agcagaagct aaaaagctaa atgatgcaca
      961 agcaccaaaa gctgacaaca aattcaacaa agaacaacaa aatgctttct atgaaatttt
     1021 acatttacct aacttaactg aagaacaacg taacggcttc atccaaagcc ttaaagacga
     1081 tccttcagtg agcaaagaaa ttttagcaga agctaaaaag ctaaacgatg ctcaagcacc
     1141 aaaagaggaa gacaacaaaa aaactggtaa agaagacggc aacaaacctg gcaaagaaga
     1201 cggcaacaag cctggtaaag aagacaacaa aaaacctggt aaagaagacg gcaacaagcc
     1261 tggtaaagaa gacaacaaca aacctggcaa agaagacggc aacaagcctg gtaaagaaga
     1321 caacaacaag cctggtaaag aagacggcaa caagcctggt aaagaagacg gcaacaaacc
     1381 tggtaaagaa gacggcaacg gagtacatgt cgttaaacct ggtgatacag taaatgacat
     1441 tgcaaaagca aacggcacta ctgctgacaa aattgctgca gataacaaat tagctgataa
     1501 aaacatgatc aaacctggtc aagaacttgt tgttgataag aagcaaccag caaaccatgc
     1561 agatgctaac aaagctcaag cattaccaga aactggtgaa gaaaatccat tcatcggtac
     1621 aactgtattt ggtggattat cattagcctt aggtgcagcg ttattagctg gacgtcgtcg
     1681 cgaactataa aaacaaacaa tacacaacga tagatatcat tttatccaaa ccaattttaa
     1741 cttatatacg ttgattaaca cattcttatt tgaaatgata agaatcatct aaatgcacga
     1801 gcaacatctt ttgttgctca gtgcattttt tattttactt acttttctaa acaacttctg
     1861 aaacgcctca acactttcta ctctgattac atatacgaca tttttagaca ttaaaaaatc
     1921 gaactagaca agatgctcat tgcatttcgt actagttcga ttcatgaata attagattta
     1981 aaatgtcatt tgaatccaag tgacaacatt atttatattt agaatattaa cgttagtata
     2041 aacgtccaaa cacaaataaa ggcaacaaat ataatactgt attttaacgt catttttaat
     2101 aatgcagatt cttcaccaac ttttttaaca gctgcagtcg caatggcaat tgattgtggt
     2161 gaaataagtt tcgctgctac accacctgca gtgttagctg ccacaagtaa tgaaccgctt
     2221 gttgaaattt gttgtgccac tgtcgcttga ataggtgcga ataaagtatt gttatttaca
     2281 actgaaccag tcataaacac accaatccaa cctaatactg gagagaataa tgggaaaatt
     2341 gctcccgctt tagcaatacc ttgtccaatt gctacagtca aaccaccgta tgtcataact
     2401 ttagcaatag ctaggatagc tgaaattgta aggatcggta accataattc tttaattgct
     2461 tcgaccaata aagcacctgc acttttccat tttaacttcg taattaaaat tgtaataatt
     2521 actgttaata aaatcgctgt cccagttgca ccaattaaat cgagacgcaa cgcaattcct
     2581 ttaggcgata aatcactcac agtatttgga attggcaatt ttattactaa actttcaagt
     2641 gcacctccag gttggaataa ttttttgaag aatggtgcac tccatactaa tacaaaggca
     2701 gttaaaatta cgaacggact ccaagcaaag acaatttctt taggcgttcg tttttgaatt
     2761 ttatgttcag acgcttccaa tctgaaaatg tttttcggtt taaatttacg acaaacaaat
     2821 gctaacacca ccattgttgc tagtgatgga ataatgtctg ctagttctgg accatggaat
     2881 attgttaata ataattgtaa tccagtatat gtaccactca ctgttaaaat gacaggtaaa
     2941 atttctttaa tacctttcat accatctaca atgaatacta aaacaaatgg aataataaag
     3001 tttaaaattg gaagtgttaa tgctgagtat ctcgcaacat ctaatgttgt aacgcctcca
     3061 cttaagttaa acgtatcaat aatactaact ggtaaaccaa ttgcaccaaa ggcacccgcc
     3121 gcaccattag caattaaaca taacatcgct gcttttaatg gttcaaatcc aagttgaatt
     3181 aataatactg cacaaatcgc aattggcaca ccaaatcctg ctgcaccttc taaaaatgcg
     3241 ttgaaacaaa atccaattaa taatagttgg attctttggt cctctgaaat acttgcaata
     3301 ctatcttgaa taatagaaaa ttgtcctgtt ttaatagaaa ctttatataa ccaaactgcc
     3361 attaaaacga tatatcctat tgggaaaata ccggcaacaa cgccttctgt aatcgcacct
     3421 gctgatacac gcgctggtaa ttcaaataca aataaagcca caatcaatgt aacaaccaaa
     3481 gttgtcaatg ctgcataaat gcctttcatt ttaaaaacgg ttaagcataa taaaaataaa
     3541 ataataggta ctgctgcaac taaggctgat aatccaacat tatcgaatgg atttacagta
     3601 agtagtgtca taatgactcc ctctctttat ataaaatatt tatcattctg attaatctac
     3661 aacctatttc aacttatatt ttgcgatgat cacatattta aaatgtaaca ctcctatatg
     3721 tgacaggcaa tcgaattttt acaaaaagtt cacaaaatat acacaatatt taactataat
     3781 aaataatata tcattttaat tataaatact agatattatt tataataata tcaggaatcc
     3841 gcttcaaaaa tgcatcatga cagtttatat ttttattgag aatctctcat tttgtgaatt
     3901 gtaggaagta aacaaaatat gacaagcgtt aaaccaatga taatgataaa tatcatatta
     3961 aaccatagta aatttaattg atgatggtgt tgtatttgcc aaatttctaa tactgtgaag
     4021 atagacatat agctcataat ctctaaattt aacgtactta aaaataattt aatcgctcga
     4081 atttcacgtg gtgtatgatt ataatgtgac catttggcaa atcgttcata gtatctacct
     4141 gcaatgaaaa atataagcca aatcactata aatgcgctat ttatcaaaag cagtacccat
     4201 ttatcagcaa agttatcagc atcccctgct aaattatagt gaataggtac tttggttggt
     4261 aattttgaat aggtcactac tgtatagcac atcatagcta agtaaataag tagacttaat
     4321 attgtaaaag acctgatttt agacattcta tcgcctcttt ctttacattt tatgtataac
     4381 actctgccta ttttaccttt taattacatt accccaaacg attaaacaat atgtaatgat
     4441 actataattg cgtcaggagt atccgcttgt taaatgtgca tagcttatat ttagctgttt
     4501 aacatgccac ataatgattc gaattattgt tttaattaaa cctttttcac atatgtataa
     4561 attttataga gcgcatgctt aatattttca tttgattcaa aggcaaaaga tagccttata
     4621 ctcttttctt tactgccata aatgtaaccc ggattgatta atattttttc tttacttaac
     4681 aattcgtaaa ataaatattt aatatctata tctattttaa agactaacca tacaaaataa
     4741 cctccacttg gaacctccca atgtgctata tccttaaata aattattgag gatgtttaac
     4801 ataaagtctc gtttatcttt taaaacatac cttaacttta ctaagtgttt atcataagac
     4861 ttatttttca acatctcata tacaaccatt tgtgacaaaa tactggatcc atagtcaatt
     4921 tgcattctta catctgccaa ttgctctatt attttttcag aagcaacaat ccaacctatt
     4981 cttattgctg gtgcaatcgt ttttgaaaaa ctgcttatat gtataacttt tcccaattta
     5041 tcataagttt tgatagcagg agttggatcg ctaaagaaaa tatctctaaa gatatcatct
     5101 tcaatgatag gaatattatg tctttcgcta taagtaatta tatttttctt ttgctcattc
     5161 gttaaagaac gacctgtcgg gttattaaac ctaggttcta tataaatcgc tttattttta
     5221 aaattaataa atctatcaat gatggtatta atttcattaa tttgattata aggaacatca
     5281 atatgtctaa aattcaattg ctcaaaaaca tttgtagagt gaatatatga tggtgtattc
     5341 gaaattatta tggcatcttg acctaaaaac ccaatagata aaagttgaat ggcatgtaaa
     5401 gcgcctgaag tgatcattac attttctcta cctacattta taccttgctt tgacattcgt
     5461 tcaacgataa tatctcttaa cttgatataa ccatagccat tattataacc aaaagataag
     5521 tcttcaatat gactggctgt attagacatg gcttttttca attgaatatg tggcattaac
     5581 gatataccca attcaccttt acttatatgt atatacgaat catctgtctc aattttatta
     5641 attaattgca ccgtatactg acttctttgt tgagaggacc ataacatcat ttcagaccac
     5701 ttatttgtaa tatgtgcttc attcaaatag tcattaacat atgttccact ccccacttta
     5761 gtatagataa atccttcagc ctctaataac tcaatacttt taataatcgt cactctattt
     5821 acgttgtatt gtatcgccaa ctgcctctgt ggaggtatct tcattccata aaaccaatcc
     5881 cctgtgatta ttttatctct tatgtcacta gcaatatctt aatatttagc catcttctcc
     5941 ctccctttta aaattggata ggtaaatcca ttcaaaattg gttgtactca ttttaaatca
     6001 tattttttaa tatagaaaca cataattaaa ggagtgatga tattaatgaa taaacttcga
     6061 gacactactt ttctatcata tttatttact atcatattgt ggggatctgc atttccaatg
     6121 ataaagattg cgttaaatga ttttagtgca gaatcattgt cggcatttcg tttaatttta
     6181 gcaacaataa ttctcttgcc gttcgtaatt ataaagaaat tgcctacccc tgaactaaga
     6241 gatatccctg ttatttttat tttaggattt tgtggatttg tgatatatca cacagcttta
     6301 aattttggtg aaactttgat tagtgcaggt atatctggaa ttctagtctc tacaacgcct
     6361 attttttcta gtgctttagc ttatatattt ttaaaagagc atttttcaaa atggaattgg
     6421 ttaagttcac tcgtcgcatt tataggaata tccattattt caataagtaa agatgattac
     6481 acaactatta atgtattagg tgtttttatt attttacttg catcttttag tgaaagtttg
     6541 tattttactt tccagaaaaa atacatagaa aaatacggct tcatcgcttt cacactatat
     6601 acaataatgg caagctcacc atttatgctt atttttattc ctgaaatcat caacgatata
     6661 cacggcgcca cttttacatc aatagtatcg gtactttatt tagctatatt ccctactata
     6721 attccatacg ttttgcttgc ttatattgtg aagtcagttg gtgtctctga tgcaacaatg
     6781 tctctttatt taacacctat cgtttcttta ttattatctt atctgttatt agacgagcta
     6841 ccaacaaccc ttgctattat aggcggaatt atcactctac taggcgttag tttaagtaac
     6901 ttctttcaaa atacataatt attccaagtc ccgcacctca gaatccaaaa acattcgagt
     6961 gataaaattt taaaaatcaa aaatataaaa atgatctaat ttcgtcaaat ttaccaatat
     7021 aaatactaat atttacaatt cacaaagggg tatagtctga gtgtattcta atacgaaagg
     7081 acttggtgga tatgtattac agttatggaa attatgaagc atttgcgcgc cctaaaaaac
     7141 ctgaaaatgt agaaaacaaa tctgcttact taatcgggtc tgggttagct tcacttgcgg
     7201 cagcttgttt cttaattagg gatggtcaaa tggaaggttc gaagattcat attttagaag
     7261 agttacctaa agcaggtggt agtcttgatg gtgaaaatat gcctttaaaa ggctatgttg
     7321 tccgcggtgg tcgtgaaatg gagaaccact ttgaatgttt gtgggactta ttcagatcta
     7381 tcccttcatt agaaatcgat aacgcgtctg tattagatga gttctattgg ctaaacaaag
     7441 aagaccctaa ctattctcgc tgtcgtgtta ttgagaaaca gggtcaacgt ttagtcacag
     7501 acggagactt cactttgact aaaacggcga ttaaagaaat tttagattta tgcttaacga
     7561 atgaagaaga tttagatgat gtcaaaataa cagatgtatt ttccgatgac ttctttaatt
     7621 caaacttttg gatttactgg aaaacgatgt ttgcatttga accgtggcat tctgctatgg
     7681 aaatgcgtcg ttatctaatg cgattcgttc atcatattgg tggtctcgca gacttttcag
     7741 ctttaaaatt cactaaatat aatcaatatg aatctttagt attacctatg gttgaatatt
     7801 taaaatcgca tggggttcaa tttgaatacg atgtaaaagt agaagatatt aaagtagatg
     7861 ttacgacaag tcaaaaaatt gcccgagaaa tattaattca ccgacatggc aaagcagagt
     7921 caattaaact gactgtagat gatcttgtct ttgtgacaaa cggtagtatt acggaaagct
     7981 ctacgtatgg tgataatgat acaccagcac caccaactga cgaattaggt ggtagttgga
     8041 cactatggaa aaatttagcg cgacaaagtc ctgaatttgg taatcctgat aagttttgcc
     8101 aaaatattcc taaaaaaagt tggtttgttt cagcaacttc tacaacaaac aataaagaga
     8161 ttatcgatac aatagaaagt atttgtaaac gtgacccact tgcaggcaaa acagttacag
     8221 gcggtattat tacaatcaat gattctgcat ggcaaatgag ttttacaatc aatcgtcagc
     8281 aacagtttaa agaccaacct gaaaatgaaa tatctacatg gatttatgcc ttatattcag
     8341 atgtaaacgg cgattatatt aaaaagccaa ttacagaatg tagtggtaat gaaatatgcc
     8401 aagaatggct gtatcactta ggtgtatcaa ctgacaaaat tgaagactta gcaaaacatg
     8461 tatctaatac gattcctgtt tatatgccat atatcacctc ttatttcatg acgcgtgcta
     8521 tcggcgacag acctttagtc gtcccgcatc aatcacaaaa tttatcattt attggtaact
     8581 ttgcagaaac agagcgagat actgtattta caacagaata ttcggttcgt actgccatgg
     8641 aagctgttta tcaattacta aatatagatc gtggtattcc agaggtcatc aatagtacat
     8701 ttgatcttcg cgtcttaatg gatgccgtat acgaactgaa tgaccatcaa gatttgcgcg
     8761 agattactaa agattcgaaa atgcagaaac tcgcattagc aggattcctt aaaaagataa
     8821 aaggtacgta cattgagaca ttattaaaag aacacaaatt gttataacga aaaccattaa
     8881 tagattttta tttggtgatt tcaaatcatg gagtgggata gaaatgatat gttcacaaac
     8941 tttatttcgt tatcccactc acatgatttt tttgatgaaa cataattaca tgattgattg
     9001 catcattttg ttaaacaatt tattgcaaac ctgccatttc acactgaaaa tttacataat
     9061 aagtgacgat attttacaag tcatatacaa ataacatata ttgttaaata attttaccta
     9121 atcttaacat taaatttaca attataagcg ataatctaaa tataaagctt atttgaggtg
     9181 aaataatgga aatgtcggtt acagaagtca ttttctcctt tttaggtggt ttaggtattt
     9241 tcctttacgg cttaaaaatc atgggagacg ggcttcaagc atcagcagga gacaggctac
     9301 gagatatttt aaacaaattt acatcaaatc cagtattagg tgttattgca ggtatcgttg
     9361 taactatttt aatacaaagt agttcaggta cgaccgttat cacaatcgga ctggtaacag
     9421 ctggatttat gacgttaaaa caagctattg gagtgataat gggtgccaat atcggaacaa
     9481 cggtaactgc atttattatc ggtatagatt taggcgaata tgcaatgcca attttagcat
     9541 taggtgcatt tttaatcttt ttctttaaac gctctaaaat caataacatt ggccgcatac
     9601 tattcggttt cggttctcta ttcttcggtc tagaatttat gggtgatgcc gttaaacctt
     9661 tagcatcatt agatggattt aagcaattaa tgcttgatat gtcatcaaat ccaatacttg
     9721 ctgtcattgt cggcgcaggg ttaacagcac tagttcaaag ttcaagtgcg acgattggta
     9781 ttttacaaga attttatcaa caagatttaa tcagcttaaa cgcagcaatc cctgtgttac
     9841 taggtgataa cattggtaca acaattacag ctatcttagc tagtttagcc ggctcaatcg
     9901 ctgcaaaacg tgcggcgctt gtacacgtca tctttaattt aatcggggta attatcttca
     9961 caattttctt gccagttgtg attcatttga ttagtttgtt acaagatgta tggcacttaa
    10021 aaccagcgat gacgattgca gtatcacatg gtaccttcaa cataacaaat actttgattc
    10081 aattaccatt tgtagcaggt ttagcatgga tagttacaaa gcttgtccca ggtaaagata
    10141 ttgctgatga ctataaacct cagcacttaa acaaagatct tgtttatcac gcacctggtg
    10201 ttgcattaca agaaactcaa aaagaattac aaaatgtggg tcagattgtc ttatcaatgt
    10261 ttgaagacat tcgcgaaatt acaaaagacg ataaaaaatt gatcaagaag cttgaacaaa
    10321 agcaccaagc tgttgaaaca atcaatgata gcattcgaaa ttatttagtt agaatttcta
    10381 caaaagccat tacgaaggca gacgttgagc gtttagcagt tatgtttgat gtcaatcgct
    10441 ctattttaaa agtagcagag ctaacagaag agtatgtcgc tcaattaaaa cgccaacatg
    10501 atgaagatat tcgcattaca gaagatgcac aacgcggtat ggataaatta ttcaaccatg
    10561 ttgctgagtc atttgataaa gccatcgaca tgttagatgt ttatgacaaa acgaaaaaag
    10621 atgaaattgt agaacgtagt agagaatcat ttaatattga acataaacta cgcaaaggtc
    10681 atattaaacg ccttaatcgt ggcgaatgta caacaaaagg cggattacta tatatcgata
    10741 tgattggtgt tcttgaacgt atcggttatc attcacgaaa tgtttctgaa gcacttgttg
    10801 gtcttaacga tgatgtacct acagatgaag aaattgcaac aactgaaatt taatttttaa
    10861 tgtcttattt atattcatat tttttaaaat tagagattca gaggcatata caaggtcaat
    10921 ccaacatcaa tggattggct tttttgttta gcaaacttta ttatcttaaa tcggctataa
    10981 acactgatat aataatgctt cattagtatg cggtaagcat gacggacact gttctcggag
    11041 tctgacctcg aatcatttag tattcacttt cacgtcgcct tcattgaagc gaattgccat
    11101 aaccttcaca ttatatatag ttccttctat ataaatgtcc aaatttttag aacaacgcaa
    11161 taaataacca tccacctaac ttatcaaaag tttaagtgga tggtttttca tttatattta
    11221 tattagtgtt aatccaatca tagatttatc tatatgcact gctctataca tttcctcatt
    11281 taatttgctt tactttcatt tatatcatta tcaaaacact tggcgtgtca tcgttattat
    11341 ttcgcatctt tgacacgttt atcatcatta ggaatcgcga ataaaattgc gataaatgcc
    11401 atgattccca ttaatacgtt aacccaaagt gcaatcatcg cacctgtatg aatgctcgtt
    11461 gcagcaactg caccaacata tacagcacca ctaattgcga caccgaatgc gccaccaagt
    11521 gatgaagcca ttttataaat acctgaagca acgccaactt tatctaacgg tgcattcgaa
    11581 atagctgtat ctgtagaagg tgttgcataa atacctaagc ctagtccgaa acataaatat
    11641 cctacgacac aactgataac ataaaatatg cctggtaaga atactaatga aataagtgca
    11701 ataccaatga ccacaatgaa tgtacctaat aacattggtc gcttagaacc cattttttgt
    11761 aataattttt caccaactcg aatcatcaat aacaccatga ttaaataagt aattgataag
    11821 tatcctgcct gcaatgctgt ataacctaaa ccttgttgca cgaatgtatt cgctacaatt
    11881 aatgtacctg caacaccgtt taataagaag ttcgaaatcg ttgcacctgt atatggttta
    11941 ttttcaaata atttaaaatc aataagtgga ttatctactt ttttctcaac atttaagaaa
    12001 ataaagaatg ctacaattac gattgcaatc aaaccaaaga accataatga tgtgtaacca
    12061 agtgctgcac ctttagtaat gacaacgttt aaacttagca acataactac tagaacaatt
    12121 agccctgcaa cgtcaaattt atgtgtattg gtaatttctg atttcgtttc aggcgtccct
    12181 ttgatgagta acattgaaag tacggcaacg ataattgaga agatgaaaat ccatctccaa
    12241 cccatagttg tcgcaactgc accaccgaag agtgaacaga taccactgcc accccaagaa
    12301 ccgatagacc aataacttaa ggcacgctga cgttcagcac cctgataata agttttcata
    12361 atggccaatg tggaaggcat aatacacgct gctgatacac cttgtataac acgacctaaa
    12421 attaataatg ccggtaaatt cgtaataata attaatgctg aaccaataat acttaacaat
    12481 aaaccgatat tcgtcatttt cacgcgccca attttatctg ctagaccacc tgctccaacg
    12541 acaaacatac ctgaaaatag tgcagttaga ctgaccgcta tactaattgt ccccatgtct
    12601 gcaccaaaac tttgttgtaa atttggtaca acatttacaa gtgattgtgc aaacaaccaa
    12661 aatgtaataa cacctaatac aatacctaag attaacttgt tgcccccgcg atacgtttca
    12721 ttcatgttag ttatctcctt taaggtaatc taaaacaact gtccctacag cttctgccga
    12781 aataagtaat gatttttctg aaatgttaaa tttaggatga tgatgtgggt aaatttcacc
    12841 atcttccaca gctgcacctg tataaataaa ggcacttgga cgttctttag cataatatgc
    12901 aaagtcttct gaaggtggtt gtggttcaca catttcaaca ccaaaatcaa ggtttgcttc
    12961 tttcaacgtc ttagccacgt actcagtaaa ctctggatca ttatataatg ctggataatc
    13021 atcgttatat tctaaggtgc aagttacacc atacatatcc tctaatcctt ttgataaacg
    13081 tttaatttct ttttcaattg ttgcttttgt agcatctgtt aatccacgta catcaccttc
    13141 aatttcaaca acatctttaa tgacattgaa ttgaccttta ccgtcaaatg aaccgattgt
    13201 gacaacaccg gtttcaaatg gacttagtcg tctagataca actgtttgta acgctgtgac
    13261 gaagtagcta cctgcaacaa tggcatcatt ggccatatgt ggtgatgaac catgaccacc
    13321 tttaccttga actttcaatt tgaagaatgc gcgtcctgtt tgaacataac caggtctgta
    13381 atacacatta cctgttttca ttgtgctcat gacgtgtaca cctaatacat gatcaacacc
    13441 gtctaataca ccattttcaa tcattgcttt agcaccacct ggtggtactt cttcagctgg
    13501 ttgatgtatc acaacgactt ttcctgtaaa actatctttc atttcagcaa gcgtctctgc
    13561 taatacaagc atgtaagctg tatgtgcatc gtgaccacat gcgtgcataa cacctttatt
    13621 ttgtgatgca aaagataatc ctgtatcttc agtaatgggt aatgcgtcaa agtctgcacg
    13681 gattgctaat gttttaccag gtttccctga atcaatcgtt actttaattc cacgtggtcc
    13741 gacattcgtt tctacttcca catctttacc tttgtaaaat tcagcgatgt atttcgccgt
    13801 ttcatcttca tgaaaagata attctggatg ctgatgtaaa taacgtctga tctcaatcat
    13861 tttgtcttct ttagatttta aagcttcaat taattgttga ttcatatcct tcatctcctt
    13921 agttacatca taaatgatta atcattattt atattgccaa caacagagat gttaaccatt
    13981 aattttttgc aattttagct ttgaatataa aaaatcacaa attatgtata tcaaaatttg
    14041 tgatttgtga tcattttatg aacttgggta acgttttact tcaattaagt gaatcccatt
    14101 cgtaatcatt ttaatgttta atgccagtgt gtccgtgata tctatatcat atacttctaa
    14161 tttcggaaaa ctcattcgat taacgtaatc tatagagtcc ttgtccatgc catgtatcgt
    14221 atgatgtttg cgccaaagat taaataacgc accattttct ttatctaagg taaaatgttt
    14281 aatcttatac ataccgtctt ctagggcatt aatgttcaaa tggatcattt ccgttgcacg
    14341 catattcatt tgattgtcca acgctaagta cggattaaaa tgctttgcat catataacaa
    14401 tatttgaaaa tttgaatcag ttcccgtgac aatacatgta tcatcagaat acaaaatatt
    14461 gcttgttaat ttattaaata gcaatgccgt gaaatagacc ggacgttttc cattatattg
    14521 atgaaacagt tcaatagaat tcatataatc ccgttcattt ctacaatgac tgacgtgcaa
    14581 atcataattc aaccaatacc cgataccctc tactttagta cttaatttta ataattgctc
    14641 aatgatgata ccacctctaa aatattcgcc gtttgtaata aatgtatcac ccgtcaatgt
    14701 attccaattg agtaaaatga gtggacgctt taggttatgg cgatgcatta agtcgataag
    14761 gtaattcgtt ttattaataa tcatttgact cgcggtttta aattcatcat cattcatttt
    14821 attaaaatca acagcatcat ttgaattggc atcaaataca aaatggttga tgtgtggctc
    14881 aagtcgtttc aacaatggta gatgtctttc cgtagcttga tctaagtgaa tgtacaagcc
    14941 accattaggg aataatgctt taaaataatc aatcatttca attaaagacg tgtgcaatgt
    15001 cgtcacatac aagttgaact tcaaatcttt tctatgactg acatgcaggg caacgtgatg
    15061 gataaatact ttgaatgcat cgatataatc tcgtgagtca tactgattca aatgcatggt
    15121 tagactaaag ttatgatcta ataaaaagtc taaacacaaa tcgatatcat aaaatatatt
    15181 cgaaatctct gcatcatacg tgaatggcgc attgagcttt ttcataatat atggcatcac
    15241 atcatatgct aatacttcat tgacttgaaa atcatgatga catgtaagca actgtgattg
    15301 atactgtgta ttgagcaaat tcctcaaata gcccacttga ataatatgat taaattgatt
    15361 tagttgttga ttagttggtt taaaggcaat ctctttatag ttcatctttt caatatcttc
    15421 aatgaaatga ttcatttctt caatgtagtc atttaaaagt aatatcaatt cacggtcgtg
    15481 ataattatgt tgtgccgatt gttgattttc agtgattgct ggacgatcac ctcgatattg
    15541 tttaggcgtt tgatgcgtaa agtgtttaaa tgttctcgca aagctcgctg cacttgaaaa
    15601 gccattttgc aatgcaatat cagtaatcgt ttcatctgtg tatgttaaat cgagtttcgc
    15661 atgctcaatt cgcgtcgtat ttaagaaatg ttggaatcct acacctagcg attctgtaaa
    15721 ctttttagac agatggctct ctgaccaccc aacgtattcg cttaattctg aaaggcttaa
    15781 atcttcatga aaatgtaact cgatatagtc gcatacttga ttcactttat catcatttaa
    15841 gatactttgg ttcgaatgat atgtacgcgg gacataatga atcatatgca taagcaactg
    15901 aatcacaagt tgttgctcag tcaatttaga caactcatta tgtcgaatgt gtgttgaaac
    15961 cagtcttgcc attatatttc tcagttgatg tatattcttg gttgtggtcg catctgttaa
    16021 gtgaaaatat agacaatgcg catcatcaaa cttgtctgcc aaatatttca tttggaattg
    16081 gatataacat atgatgccat cttgttgaag ttgaaatcga tacaagtcac ggtggttaat
    16141 gatgaaaatg tcgccactgt tgcattgcgt catgttattt tcatcataaa tatgtgcctc
    16201 acctttaata acaaaaccaa tcattaaacc attgagcctt ttgaaatctg gcatgctttc
    16261 agtttctact cgaattaaat aatcacgttg catactatcc ctcaattcag taatatgaat
    16321 acgtttattt tacattattt tacagcaaca tatttgaaat tcgtgttgaa tcgtgtggga
    16381 ggcaggagat tatttatcct cacttggttc aagatgtaga ctatcagtaa aaaaagtatt
    16441 ttcacctttt ttctccacaa aagtaaattc aatgtcttta tatccaactg atgaaccttt
    16501 taagtctccc gaacctttca acaataactt tggtgcttta ttcgttggta ttttatatct
    16561 ttttcgtaat tgttttacat tatagtcatc attagttaat tgatattttg ctgaataact
    16621 cggtacctct ggattatatg atatatcgcc gtctttgtac ttcgacaaat ctttaaagct
    16681 gccatattgc gcgaagaact taaaattctc gatttctttt tttatatttt cgtctttgat
    16741 acctttagtt ggaatgattt tattgtctac cattttaacc ggatattctt tatctttact
    16801 cttaggtcta ccatcttcat catgaagtgt ttcactcact atatacttcc cggttgtagt
    16861 cttagtgttt ctattcatat atagaaccat accttttgat ttcatacgtt cccctttagg
    16921 ttgaacaacc atttcagaat taataatcca tgtgccttta tcgtttttat caaattgatc
    16981 atcacgatag ccttctttat cgtataaatc ctctagattt ttaatcggat acatactcaa
    17041 tgttttttca aaacttttct ttatttccgc ttctttaccc atgccacaac cagcagtgaa
    17101 actaatgact aaaaataaga aactaatacc taacactaat ttatttaaac gtttcatcac
    17161 cttacaactc tcttcttttt attttgtttc cttgaatcga tttcatactc tttattagtc
    17221 acgattattc atcattactc ggtttaaaat gtaggttagc tgtaaagtaa gtgttttctc
    17281 cttttttctc tacgaaagta aattcaattt ttttgtatcc aattgatgaa ccatctaaat
    17341 ttcctgaacc ttttaacaat aactttggag ctttattcgt cggtatatca tatcttttac
    17401 gtaattgctt tacattataa tcatcattgg ttagttgata ttttgctgaa taaatcgggg
    17461 cttctggatt atatgaaata tctccacctt tatacttcgt caaatcttta aaattgccat
    17521 actgcgcgaa aaacttaaag ttttcgattt ctttttttat tttttcatct ttgatatctt
    17581 tagttggaat gattttattg tcaatcattt taacgggata ttctatttgc ttatcctgag
    17641 gtctgccatt ttcatccttc tttatcgcat taacatagta aaatcctttt gcactccttg
    17701 tatttctatt taacttcaaa accatacctt ttatatttaa ggcttctccc tttttctgaa
    17761 ttgccatttc agaactaata gtccacgttc ctttatcatt tttatcaaat tcgtcatcac
    17821 gatagccttc tttatcgtat aaatcctcta gatttttaat tggatacata ctcaatgttt
    17881 tttcaaaact tttctttatt tccgcttctt tccctgtacc acaaccagca acgatactga
    17941 tgattaatat caaaaaactg atgtacaata ccaatgtatt taatcgtttc ataatttcac
    18001 aatcctattt ctttttattg tttttcctgg atcgatttca taatttgatc gaatcatgat
    18061 tatttatcct cacttggatt aaaatgtagc ccatcactaa agtatatatt ttctcccttt
    18121 ttctctacga aagtaaattc aatttttttg tagccaattg atgaaccttt taaattccct
    18181 gtacctttca acaatagctt tggtgcttta ttcgttggta tattatatct ttttcgtaat
    18241 tgttttacat tatagtcatc attagttaat tgatattttg ctgaataact cggcacctct
    18301 ggattgtatg atatatcgcc gtctttgtac ttcgacaaat ctttaaagtc gccatattga
    18361 acaaagaact taaagttttc gatttctttt tttatttttt catctttaat ttctttagtt
    18421 ggaatgattt tattatcgac cattttaacc ggatatcttt tttcgttatc acgatgtata
    18481 cctttatctt ctctttctat aacatcaaca tagtaatagc catttgttgt tcttgtattt
    18541 ctattcatat ataaaaccat gccttttata ttcatatctt ttccattaga ttgaatcgac
    18601 attgacgaac gaacaatcca tgttccctta tcatttttat caaattgatc atcacgatag
    18661 ccttctttat cgtataaatc ctctagattt ttaatcggat acatactcaa cgttttttca
    18721 aaacttttct ttatttccgc ttctttccct gtaccacaac cagcaaagat actgatgatt
    18781 aaaaacagaa aactaatacc tagcactaat ttatttacac gttttatcat ttcatcactc
    18841 tctttttccg ggttcgattt catactcttt attggtcacg tttatttatc ctcacttggt
    18901 tcaagatgta gactatcact aaagtatata ttttcacctt ttttctccac gaaagtaaat
    18961 tcaatatctt tatatccaac tgatgagcct tttaaattac ctgtaccttt caacaataac
    19021 tttggtgctt tattcgtcgg tatatcatat cttttacgta attgttttac attataatca
    19081 tcattagtta cttgatattt tgctgaataa cttggcacct ctggattgta tgaaatatct
    19141 ccatctttgt acttcgacaa atctttaaag tttccatatt gaacaaagaa cttaaagttt
    19201 tcgatttctt tttttatgtt tttatcttta atttcttttg ttggaatgat tttattatcg
    19261 accattttaa ccggatatct tttttcattg tcgtgcggtt ttccttcatc ctcgtcctta
    19321 gtcacatcga catagtagta accatttgtt gttttggtat ttctattcat atatagaacc
    19381 atgcctttag ctaccatatc ttcattatta ggttgaataa ccatttcaga atttataatc
    19441 catgtacctt tatcattttt atcaaactga tcatcacgat agccttcctt atcgtataaa
    19501 tcctctagat ttttaatagg gtacatactc aatgtttttt caaagctttt cttaacttcc
    19561 gcttctttac ctatgccaca accagcagtg atactaatga ctaaaaacag aaaaataatg
    19621 cctaacacta atttgtttaa tcgtttcatc atcttacaac tctcttcttt ttaaaatga
//
LOCUS       DAHHSC010000028        19395 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.028, whole genome shotgun sequence.
ACCESSION   DAHHSC010000028 DAHHSC010000000
VERSION     DAHHSC010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 19395)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19395)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19395
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.028"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            217..525
                     /gene="rpsJ"
                     /locus_tag="NBI57_000777"
     CDS             217..525
                     /gene="rpsJ"
                     /locus_tag="NBI57_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001118669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HCD1821742.1"
                     /translation="MAKQKIRIRLKAYDHRVIDQSAEKIVETAKRSGADVSGPIPLPT
                     EKSVYTIIRAVHKYKDSREQFEQRTHKRLIDIVNPTPKTVDALMGLNLPSGVDIEIKL
                     "
     gene            553..1215
                     /gene="rplC"
                     /locus_tag="NBI57_000778"
     CDS             553..1215
                     /gene="rplC"
                     /locus_tag="NBI57_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000160215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HCD1821743.1"
                     /translation="MTKGILGRKIGMTQVFGENGELIPVTVVEAKENVVLQKKTVEVD
                     GYNAIQVGFEDKKAYKKDAKSNKYANKPAEGHAKKADAAPKRFIREFRNVDVDAYEVG
                     QEVSVDTFVAGDVIDVTGVSKGKGFQGAIKRHGQSRGPMSHGSHFHRAPGSVGMASDA
                     SRVFKGQKMPGRMGGNTVTVQNLEVVQVDTENKVILVKGNVPGPKKGLVEIRTSIKKG
                     NK"
     gene            1242..1865
                     /gene="rplD"
                     /locus_tag="NBI57_000779"
     CDS             1242..1865
                     /gene="rplD"
                     /locus_tag="NBI57_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015900976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HCD1821744.1"
                     /translation="MANYDVLKLDGTKSGSIELSDAVFGIEPNNSVLFEAINLQRASL
                     RQGTHAVKNRSAVSGGGRKPWKQKGTGRARQGTIRAPQWRGGGIVFGPTPRSYAYKMP
                     KKMRRLALRSALSFKAQENGLTVVDAFNFEAPKTKEFKNVLSTLEQPKKVLVVTENED
                     VNVELSARNIPGVQVTTAQGLNVLDITNADSLVITEAAAKKVEEVLG"
     gene            1865..2140
                     /gene="rplW"
                     /locus_tag="NBI57_000780"
     CDS             1865..2140
                     /gene="rplW"
                     /locus_tag="NBI57_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002506966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HCD1821745.1"
                     /translation="MEARDILKRPVITEKSSEAMAEDKYTFDVDTRVNKTQVKMAVEE
                     IFNVKVASVNIMNYKPKKKRMGRYQGYTNKRRKAIVTLKEGSIDLFN"
     gene            2173..3006
                     /gene="rplB"
                     /locus_tag="NBI57_000781"
     CDS             2173..3006
                     /gene="rplB"
                     /locus_tag="NBI57_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HCD1821746.1"
                     /translation="MAIKKYKPITNGRRNMTSLDFAEITKTTPEKSLLKPLPKKAGRN
                     NQGKLTVRHHGGGHKRQYRVIDFKRNKDGINAKVDSIQYDPNRSANIALVVYADGEKR
                     YIIAPKGLEVGQIVESGAEADIKVGNALPLQNIPVGTVVHNIELKPGKGGQIARSAGA
                     SAQVLGKEGKYVLIRLRSGEVRMILSTCRATIGQVGNLQHELVNVGKAGRSRWKGIRP
                     TVRGSVMNPNDHPHGGGEGRAPIGRPSPMSPWGKPTLGKKTRRGKKSSDKLIVRGRKK
                     K"
     gene            3073..3351
                     /gene="rpsS"
                     /locus_tag="NBI57_000782"
     CDS             3073..3351
                     /gene="rpsS"
                     /locus_tag="NBI57_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HCD1821747.1"
                     /translation="MARSIKKGPFVDEHLMKKVEAQEGSEKKQVIKTWSRRSTIFPNF
                     IGHTFAVYDGRKHVPVYVTEDMVGHKLGEFAPTRTFKGHVADDKKTRR"
     gene            3380..3733
                     /gene="rplV"
                     /locus_tag="NBI57_000783"
     CDS             3380..3733
                     /gene="rplV"
                     /locus_tag="NBI57_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000387528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HCD1821748.1"
                     /translation="MEAKAVARTIRIAPRKVRLVLDLIRGKNAAEAIAILKLTNKASS
                     PVIEKVLMSALANAEHNYDMNTDELVVKEAYANEGPTLKRFRPRAQGRASAINKRTSH
                     ITIVVSDGKEEAKEA"
     gene            3757..4410
                     /gene="rpsC"
                     /locus_tag="NBI57_000784"
     CDS             3757..4410
                     /gene="rpsC"
                     /locus_tag="NBI57_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HCD1821749.1"
                     /translation="MGQKINPIGLRVGIIRDWEAKWYAEKDFASLLHEDLKIRKFIDN
                     ELKEASVSHVEIERAANRINIAIHTGKPGMVIGKGGSEIEKLRNKLNALTDKKVHINV
                     IEIKKVDLDARLVAENIARQLENRASFRRVQKQAITRAMKLGAKGIKTQVSGRLGGAD
                     IARAEQYSEGTVPLHTLRADIDYAHAEADTTYGKLGVKVWIYRGEVLPTKNTSGGGK"
     gene            4413..4847
                     /gene="rplP"
                     /locus_tag="NBI57_000785"
     CDS             4413..4847
                     /gene="rplP"
                     /locus_tag="NBI57_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HCD1821750.1"
                     /translation="MLLPKRVKYRRQHRPKTTGRSKGGNYVTFGEFGLQATTTSWITS
                     RQIESARIAMTRYMKRGGKVWIKIFPHTPYTKKPLEVRMGAGKGAVEGWIAVVKPGRI
                     LFEVAGVSEEVAREALRLASHKLPVKTKFVKREELGGETNES"
     gene            4837..5046
                     /gene="rpmC"
                     /locus_tag="NBI57_000786"
     CDS             4837..5046
                     /gene="rpmC"
                     /locus_tag="NBI57_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002509269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HCD1821751.1"
                     /translation="MKAKEIRDLTTSEIEEQIKSSKEELFNLRFQLATGQLEETARIR
                     TVRKTIARLKTVAREREIEQSKANQ"
     gene            5070..5333
                     /gene="rpsQ"
                     /locus_tag="NBI57_000787"
     CDS             5070..5333
                     /gene="rpsQ"
                     /locus_tag="NBI57_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011983253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HCD1821752.1"
                     /translation="MSERNDRKVYVGKVVSDKMDKTITVLVETYKTHKLYGKRVKYSK
                     KYKTHDENNSAKLGDIVKIQETRPLSATKRFRLVEIVEESVII"
     gene            5365..5733
                     /gene="rplN"
                     /locus_tag="NBI57_000788"
     CDS             5365..5733
                     /gene="rplN"
                     /locus_tag="NBI57_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012727667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HCD1821753.1"
                     /translation="MIQQETRLKVADNSGAREVLTIKVLGGSGRKTANIGDVIVCTVK
                     NATPGGVVKKGDVVKAVIVRTKSGVRRNDGSYIKFDENACVIIRDDKGPRGTRIFGPV
                     ARELREGNFMKIVSLAPEVL"
     gene            5769..6086
                     /gene="rplX"
                     /locus_tag="NBI57_000789"
     CDS             5769..6086
                     /gene="rplX"
                     /locus_tag="NBI57_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HCD1821754.1"
                     /translation="MHIKKGDNVKVIAGKDKGKEGKVIATLPKKDRVVVEGVNIMKKH
                     QKPTQLNPEGGILETEAAIHVSNVQLLDPKTNEPTRVGYKFVDGKKVRIAKKSGEEIK
                     SNN"
     gene            6113..6652
                     /gene="rplE"
                     /locus_tag="NBI57_000790"
     CDS             6113..6652
                     /gene="rplE"
                     /locus_tag="NBI57_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011275133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HCD1821755.1"
                     /translation="MNRLKEKFNTEVTENLMKKFNYSSVMEVPKIDKIVVNMGVGDAV
                     QNSKVLDNAVEELELITGQKPLVTKAKKSIATFRLREGMPIGAKVTLRGERMYEFLDK
                     LISVSLPRVRDFQGVSKKAFDGRGNYTLGVKEQLIFPEIDYDKVSKVRGMDIVIVTTA
                     NTDEEARELLANFGMPFRK"
     gene            6675..6860
                     /locus_tag="NBI57_000791"
     CDS             6675..6860
                     /locus_tag="NBI57_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HCD1821756.1"
                     /translation="MAKTSMVAKQQKKQKYAVREYTRCERCGRPHSVYRKFKLCRICF
                     RELAYKGQIPGVRKASW"
     gene            6892..7290
                     /gene="rpsH"
                     /locus_tag="NBI57_000792"
     CDS             6892..7290
                     /gene="rpsH"
                     /locus_tag="NBI57_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000178880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HCD1821757.1"
                     /translation="MTMTDPIADMLTRVRNANMVRHEKLELPASNIKKEIAEILKSEG
                     FIKNVEYVEDDKQGVLRLFLKYGQNDERVITGLKRISKPGLRVYAKASEMPKVLNGLG
                     IALVSTSEGVITDKEARKRNVGGEIIAYVW"
     gene            7315..7851
                     /gene="rplF"
                     /locus_tag="NBI57_000793"
     CDS             7315..7851
                     /gene="rplF"
                     /locus_tag="NBI57_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HCD1821758.1"
                     /translation="MSRVGKKIIDIPSDVTVTFDGNHVTVKGPKGELSRTLNERMTFK
                     QEENTIEVVRPSDSKEDRTNHGTTRALLNNMVQGVSQGYVKVLELVGVGYRAQMQGKD
                     LILNVGYSHPVEIKAEENITFSVEKNTVVKVEGISKEQVGALASNIRSVRPPEPYKGK
                     GIRYQGEYVRRKEGKTGK"
     gene            7882..8241
                     /gene="rplR"
                     /locus_tag="NBI57_000794"
     CDS             7882..8241
                     /gene="rplR"
                     /locus_tag="NBI57_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011275136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HCD1821759.1"
                     /translation="MISKIDKNKVRLKRHARVRTNLSGTAEKPRLNVYRSNKHIYAQI
                     IDDNKGVTLAQASSKDSDIATTATKVELATKVGEAIAKKAADKGIKEIVFDRGGYLYH
                     GRVKALAEAARESGLEF"
     gene            8262..8762
                     /gene="rpsE"
                     /locus_tag="NBI57_000795"
     CDS             8262..8762
                     /gene="rpsE"
                     /locus_tag="NBI57_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016426364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HCD1821760.1"
                     /translation="MARREEETKEFEERVVTINRVAKVVKGGRRFRFTALVVVGDKNG
                     RVGFGTGKAQEVPEAIKKAVEAAKKDLVVVPRVEGTTPHTITGRYGSGSVFMKPAAPG
                     TGVIAGGPVRAVLELAGITDILSKSLGSNTPINMVRATIDGLQNLKNAEDVAKLRGKT
                     VEELYN"
     gene            8779..8958
                     /gene="rpmD"
                     /locus_tag="NBI57_000796"
     CDS             8779..8958
                     /gene="rpmD"
                     /locus_tag="NBI57_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HCD1821761.1"
                     /translation="MAKLQITLTRSVIGRPETQRKTVEALGLKKTNSSVVVEDNPAIR
                     GQINKVKHLVTVEEK"
     gene            8975..9415
                     /gene="rplO"
                     /locus_tag="NBI57_000797"
     CDS             8975..9415
                     /gene="rplO"
                     /locus_tag="NBI57_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HCD1821762.1"
                     /translation="MKLHELKPAEGSRKERNRVGRGVATGNGKTSGRGHKGQKARSGG
                     GVRPGFEGGQLPLFRRLPKRGFTNINRKEYAIVNLDQLNKFEDGTEVTPALLVESGVV
                     KNEKSGIKILGNGSLDKKLTVKAHKFSASAAEAIDAKGGAHEVI"
     gene            9415..10707
                     /gene="secY"
                     /locus_tag="NBI57_000798"
     CDS             9415..10707
                     /gene="secY"
                     /locus_tag="NBI57_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HCD1821763.1"
                     /translation="MIQTLVNFFRTKEVRNKIFFTLAMLVIFKIGTYIPAPGVNPAAF
                     DNPQGSQGATELLNTFGGGALKRFSIFAMGIVPYITASIVMQLLQMDIVPKFSEWAKQ
                     GEVGRRKLNNVTRYLAISLAFIQSIGMAFQFNNYLKGALIINQSIMSYLLIALVLTAG
                     TAFLIWLGDQITQFGVGNGISIIIFAGILSTLPASLIQFGQTAFVGQEDTSLAWLKVL
                     GLLVSLILLTVGAIYVLEAVRKIPIQYAKKQTAQRLGSQATYLPLKVNSAGVIPVIFA
                     MAFFLLPRTLTLFYPDKEWAQNIANAANPSSNVGMVVYIVLIILFTYFYAFVQVNPEK
                     MADNLKKQGSYVPGIRPGEQTKKYITKVLYRLTFVGSIFLAVISILPILATKFMGLPQ
                     SIQIGGTSLLIVIGVAIETMKSLEAQVSQKEYKGFGGR"
     gene            10724..11371
                     /locus_tag="NBI57_000799"
     CDS             10724..11371
                     /locus_tag="NBI57_000799"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HCD1821764.1"
                     /translation="MNIILMGLPGAGKGTQASEIVKKFPIPHISTGDMFRKAIKEETE
                     LGKEAKSYMDRGELVPDEVTVGIVKERISEDDAKKGFLLDGFPRTIEQAEALNNIMSE
                     LDRNIDAVINIEVPEEELMNRLTGRRICESCGTTYHLVFNPPKVEGICDIDGGKLYQR
                     EDDNPETVANRLSVNIKQSKPILDFYDQKGVLKNIDGSKDISDVTKDVIDILDHL"
     gene            11564..11782
                     /gene="infA"
                     /locus_tag="NBI57_000800"
     CDS             11564..11782
                     /gene="infA"
                     /locus_tag="NBI57_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017185218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HCD1821765.1"
                     /translation="MAKQDVIELEGTVLDTLPNAMFKVELENGHEILAHVSGKIRMNY
                     IRILPGDKVTVEMSPYDLTRGRITYRYK"
     gene            11814..11927
                     /gene="rpmJ"
                     /locus_tag="NBI57_000801"
     CDS             11814..11927
                     /gene="rpmJ"
                     /locus_tag="NBI57_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HCD1821766.1"
                     /translation="MKVRPSVKPICEKCKVIKRKGKVMVICENPKHKQRQG"
     gene            11950..12315
                     /gene="rpsM"
                     /locus_tag="NBI57_000802"
     CDS             11950..12315
                     /gene="rpsM"
                     /locus_tag="NBI57_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015900957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HCD1821767.1"
                     /translation="MARIAGVDIPREKRVVISLTYIYGIGTSTAQKILEEANVSADTR
                     VKDLTDDELGRIREVVDGYKVEGDLRRETNLNIKRLMEISSYRGIRHRRGLPVRGQKT
                     KNNARTRKGPVKTVANKKK"
     gene            12339..12728
                     /gene="rpsK"
                     /locus_tag="NBI57_000803"
     CDS             12339..12728
                     /gene="rpsK"
                     /locus_tag="NBI57_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016998822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HCD1821768.1"
                     /translation="MARKQVSRKRRVKKNIENGVAHIRSTFNNTIVTITDEFGNALSW
                     SSAGALGFKGSKKSTPFAAQMASETASKSAMEHGLKTVEVTVKGPGPGRESAIRALQS
                     AGLEVTAIRDVTPVPHNGCRPPKRRRV"
     gene            12803..13747
                     /locus_tag="NBI57_000804"
     CDS             12803..13747
                     /locus_tag="NBI57_000804"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011302606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HCD1821769.1"
                     /translation="MIEIEKPRIETIEISEDAKFGKFVVEPLERGYGTTLGNSLRRIL
                     LSSLPGAAVKYIEIEGVLHEFSAVDNVVEDVSTIIMNIKQLALKIYSEEDKTLEIDVR
                     DEGEVTASDITHDSDVEILNPELKIATVSKGGHLKIRLVANKGRGYALAEQNNTSDLP
                     IGVIPVDSLYSPVERVNYTVENTRVGQSSDFDKLTLDVWTNGSITPQESVSLAAKIMT
                     EHLNIFVGLTDEAQNAEIMIEKEEDQKEKVLEMSIEELDLSVRSYNCLKRAGINSVQE
                     LADKSEADMMKVRNLGRKSLEEVKYKLEDLGLGLRKED"
     gene            13764..14132
                     /gene="rplQ"
                     /locus_tag="NBI57_000805"
     CDS             13764..14132
                     /gene="rplQ"
                     /locus_tag="NBI57_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002450507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HCD1821770.1"
                     /translation="MGYRKLGRTSDQRKAMLRDLATSLIISERIETTEARAKEVRSVV
                     EKLITLGKKGDLASRRNAAKTLRNVEILNEDETTQTALQKLFGEIAERYTERQGGYTR
                     ILKQGPRRGDGAESVIIELV"
     gene            14670..15479
                     /locus_tag="NBI57_000806"
     CDS             14670..15479
                     /locus_tag="NBI57_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HCD1821771.1"
                     /translation="MEDKNSVIVFKNVSFQYQSDASFTLKDVSFNIPKGQWTSIVGHN
                     GSGKSTIAKLMIGIEKVKSGEIFYNNQAITDDNFEKLRKDIGIVFQNPDNQFVGSIVK
                     YDVAFGLENHAVPHDEMHRRVSEALKQVDMLERADYEPNALSGGQKQRVAIASVLALN
                     PSVIILDEATSMLDPDARQNLLDLVRKVKSEHNITIISITHDLSEAMEADHVIVMNKG
                     TVYKEGTATEIFDHAEELTRIGLDLPFPIKINQMLGHQTSFLTYEGLVDQL"
     gene            15476..16336
                     /locus_tag="NBI57_000807"
     CDS             15476..16336
                     /locus_tag="NBI57_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HCD1821772.1"
                     /translation="MTIRFDNVSYTYQKGTPYQHQAIHDVNTEFEQGKYYAIVGQTGS
                     GKSTLIQNINALLKPTTGTVTVDDITITHKTKDKYIRPVRKRIGMVFQFPESQLFEDT
                     VEREMIFGPKNFKMNLDEAKNYAHRLLMDLGFSRDVMSQSPFQMSGGQMRKIAIVSIL
                     AMNPDIIVVDEPTAGLDPQSKRQVMRLLKSLQTDENKAIILISHDMNEVARYADEVIV
                     MKEGSIVSQTSPKELFKDKKKLADWHIGLPEIVQLQYDFEQKYQTKLKDIALTEEAFV
                     SLYKEWQHEK"
     gene            16326..17132
                     /locus_tag="NBI57_000808"
     CDS             16326..17132
                     /locus_tag="NBI57_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HCD1821773.1"
                     /translation="MKNKLIIGRYLPINSFVHHLDPRAKLMFVFLFIILIFFCHSPLT
                     YLWVFALILFIMRLAKIQLWFLIKGLTPIFFFLIFTLMMHIFLTKGGYVLVEWHGITI
                     ETNGILEGLYISLRLIGIVMIATIMTLSTSPIDLTDAFERLLAPLKMFKLPVHQLSMI
                     MSIALRFIPTLMDELDKIILAQKSRGSEISSGNIATRIKSFIPLLVPLFISAFQRAEE
                     LAVAMEVRGYDANVKRTSYRQLKWQLRDTISLTMIIPIAIILFVLKYSGV"
     gene            17137..17940
                     /gene="truA"
                     /locus_tag="NBI57_000809"
     CDS             17137..17940
                     /gene="truA"
                     /locus_tag="NBI57_000809"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HCD1821774.1"
                     /translation="MRILVEIAYQGNNFLGFQIQQNGRTVQQQFEKLLQRMHKRHVRI
                     HPSSRTDRGVHAIQQYFHFDTELNIPMSQWQYAMNRTLPDDIYVNNVVTVDDDFHCRY
                     DCVGKRYRYKVYQAQHRDPFQSGLKTFIPETLDLDKMNRAAQQFIGTHDFTGFCSQKT
                     EVESKVRTLYQSEIVKTDDGFDYIVTGSGFLYNMVRVLVAFLIEVGKGRHEISDVPKL
                     LESKNRKNVPFTAPAEGLYLEKIYLDENELLKDFGNDIKIHRKKSLQND"
     gene            18180..18617
                     /gene="rplM"
                     /locus_tag="NBI57_000810"
     CDS             18180..18617
                     /gene="rplM"
                     /locus_tag="NBI57_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019468667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HCD1821775.1"
                     /translation="MRQTFMANESNIERKWYVIDAEGQTLGRLSSEVASILRGKNKVT
                     YTPHVDTGDYVIVINASKIEFTGNKETDKVYYRHSNHPGGIKSITAGELRRTNPERLI
                     ENSIKGMLPSTRLGEKQGKKLFVYGGAEHPHAAQQPENYELRG"
     gene            18631..19029
                     /gene="rpsI"
                     /locus_tag="NBI57_000811"
     CDS             18631..19029
                     /gene="rpsI"
                     /locus_tag="NBI57_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HCD1821776.1"
                     /translation="MTLAQVEYRGTGRRKNSVARVRLVPGEGNITVNNRDVREYLPFE
                     SLILDLNQPFDVTETKGNYDVLVNVHGGGFTGQAQAIRHGIARALLEADPEYRGSLKR
                     AGLLTRDPRMKERKKPGLKAARRSPQFSKR"
     gene            complement(19207..>19395)
                     /locus_tag="NBI57_000812"
     CDS             complement(19207..>19395)
                     /locus_tag="NBI57_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016170730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1821777.1"
                     /translation="TEIIHNGKSIFDLYDNYEDVYKLLNWIKLIIIEYISNIVKKNIQ
                     TFDDLEKERKKIINSLGI"
BASE COUNT         6961 a         3112 c         3824 g         5498 t
ORIGIN      
        1 gtaaaatata aaagtatgtg ttagacagat agaaaaatta tgtgaaaagc cttgatttat
       61 cgatgttttt cgtgtaatat aactaacgtt ggactttaag aaaagcgatg aagcgaaagg
      121 ttactgacac acccggccgc tttgccatgg cgctgtgtaa gatagttttc gtggagaagt
      181 ctatcactaa atgtagacga ataaggaggg aaaattatgg caaaacaaaa aatcagaatc
      241 agattaaaag cttatgatca ccgcgtgatt gatcaatcag cagagaagat tgtagaaaca
      301 gcgaaacgtt ctggtgcaga tgtttctgga ccaattccgt taccaactga gaaatcagtt
      361 tacacaatca tccgtgccgt gcataagtat aaagattcac gtgaacaatt cgaacaacgt
      421 acacacaaac gtttaatcga tattgtaaac ccaacaccaa aaacagttga cgctttaatg
      481 ggcttaaact taccatctgg tgtagacatc gaaatcaaat tataatagac aattttagga
      541 ggtggacttt cgatgaccaa aggaatctta ggaagaaaaa ttgggatgac acaagtattc
      601 ggagaaaacg gtgaattaat ccctgtaaca gtagtagaag ctaaagaaaa tgttgtatta
      661 caaaagaaaa ctgtagaagt tgatggatac aacgcaatcc aagttggatt tgaagacaaa
      721 aaagcataca aaaaagatgc aaaatctaat aaatatgcta ataaaccagc tgaaggtcac
      781 gctaaaaaag ctgacgcagc acctaagcgc ttcattcgtg aattccgcaa tgtagacgtg
      841 gatgcttacg aagtaggtca agaagtctca gtagatactt ttgtagctgg cgacgttatt
      901 gacgtaacag gcgtatcaaa aggtaaaggt ttccaaggtg caattaaacg ccacggacaa
      961 tctcgtggac ctatgtcaca cggttctcat ttccacagag caccaggttc tgtaggtatg
     1021 gcttcagatg cttctagagt atttaaaggc caaaaaatgc caggacgtat gggtggaaac
     1081 actgtaactg ttcaaaactt agaagtagtt caagttgaca cagaaaacaa agttatctta
     1141 gtaaaaggta acgtacctgg acctaaaaaa ggtttagtag aaatcagaac ttcaattaaa
     1201 aaaggtaata aataataaga agtgaaagga ggaaatgcat aatggctaat tatgatgttt
     1261 taaaattaga cggaactaaa tcaggttcaa tcgaattaag cgatgcagta ttcggtattg
     1321 agccaaataa tagcgtttta ttcgaagcta ttaatttaca acgtgcttca ttacgtcaag
     1381 gtacgcatgc tgttaagaat cgttcagcag taagcggtgg cggacgtaaa ccatggaagc
     1441 aaaaaggaac aggtcgtgct cgtcaaggta caatccgtgc tccacaatgg cgtggcggtg
     1501 gtatcgtatt cggaccaact ccaagaagtt atgcatacaa aatgcctaag aaaatgcgtc
     1561 gtttagcttt acgttcagca ttatctttca aagctcaaga gaatggctta actgtagttg
     1621 acgcattcaa cttcgaagct ccaaaaacta aagaattcaa aaatgtatta tctacattag
     1681 aacaacctaa aaaagtatta gtagttactg aaaacgaaga tgtaaatgtt gaattatcag
     1741 cacgcaacat ccctggcgtt caagtgacaa ctgctcaagg tttaaatgtt ttagatatca
     1801 ctaatgctga cagcttagta attactgaag ctgctgctaa aaaagttgag gaggtgctcg
     1861 gataatggaa gcaagagata ttcttaagcg ccccgtaatc actgagaaat cttctgaagc
     1921 aatggctgaa gacaaataca ctttcgacgt tgatactcgt gttaacaaaa cacaagtaaa
     1981 aatggcagtt gaagaaatct tcaacgtaaa agttgcaagt gttaatatca tgaattacaa
     2041 acctaagaaa aaacgtatgg gccgttacca aggctataca aacaaaagaa gaaaagcgat
     2101 tgtaactctt aaagaaggat caatcgactt atttaactaa taaaacaatt accattaagg
     2161 aggtaagcga caatggctat taaaaagtat aagccaataa caaatggtcg tcgtaatatg
     2221 acttcgttag atttcgcaga aatcacgaaa actacacctg aaaagtcatt attaaaaccg
     2281 ctaccgaaaa aagcgggacg taacaaccaa ggtaaattga ctgtaagaca ccatggtggt
     2341 ggacacaaac gtcaataccg tgttatcgat tttaaacgta acaaagatgg tatcaatgca
     2401 aaagttgatt ctattcaata tgatccaaac cgctcagcaa acatcgcttt agttgtatat
     2461 gcagacggtg aaaaacgata tatcattgct cctaaaggat tagaagtagg tcaaatcgtt
     2521 gaaagtggtg ctgaagctga catcaaagtt ggtaacgcat taccattaca aaacattcca
     2581 gttggtacag tagtacacaa catcgagctt aaacctggta aaggtggaca aatcgctcgt
     2641 tcagctggtg caagtgctca agtacttggt aaagaaggta aatacgtatt aatcagatta
     2701 agatctggtg aagttcgtat gatcttatct acttgccgtg ctacaatcgg tcaagttggt
     2761 aacctacaac acgaattagt taacgttggt aaagccggac gttcaagatg gaaaggtatc
     2821 cgtccaacag ttcgtggttc tgtaatgaac cctaacgatc acccacacgg tggtggtgaa
     2881 ggtcgtgctc ctatcggtag accatctcca atgtcaccat ggggtaaacc tacgcttggt
     2941 aagaaaactc gtcgtggtaa aaaatcatca gacaaactta tcgttcgtgg acgtaagaaa
     3001 aaataatata atcaacttat ttgggtgtgc ggcttaaagc tgcacgcaca taataagaag
     3061 ggaggcgccc aaatggctcg tagtattaaa aaaggacctt tcgtcgatga gcatttaatg
     3121 aaaaaagttg aagctcaaga aggaagcgaa aagaaacaag taatcaaaac atggtcacgt
     3181 cgttctacaa ttttccctaa tttcatcgga catacttttg cagtatacga cggacgtaaa
     3241 cacgtacctg tatatgtaac tgaagatatg gtaggtcata aattaggtga gtttgctcct
     3301 actcgtacat tcaaaggaca cgttgcagac gacaagaaaa caagaagata atatctatta
     3361 agtagaggag gacatcctaa tggaagcaaa agcggttgct agaacaataa gaatcgcacc
     3421 tcgtaaagta agactagttc ttgacttaat cagaggtaaa aatgctgctg aagctattgc
     3481 aattttaaaa ttaacaaaca aagcttcatc accagtaatt gaaaaagtat taatgtccgc
     3541 tttagctaat gctgaacata actatgacat gaacacagat gaattagtag ttaaagaagc
     3601 atatgctaac gaaggaccaa cattaaaacg tttccgtcca cgtgcgcaag gtcgtgcaag
     3661 tgcgattaac aaacgtacaa gccacattac aatcgtcgta agtgacggta aagaagaagc
     3721 taaagaagct taattaactt ttaaggaggg aatactgtgg gtcaaaaaat taatccaatc
     3781 ggacttcgtg ttggtattat ccgtgattgg gaagctaaat ggtatgctga aaaagacttc
     3841 gcttcacttt tacacgaaga tttaaaaatc cgtaaattta ttgataatga attaaaagaa
     3901 gcatcagttt ctcacgtaga gattgaacgt gctgcaaacc gtatcaacat tgcaattcat
     3961 actggtaaac ctggtatggt aattggtaaa ggcggttcag aaatcgaaaa attacgcaac
     4021 aaattaaatg cgttaactga taaaaaagta cacatcaacg taattgaaat caaaaaagtt
     4081 gatcttgacg ctcgtttagt agctgaaaac atcgcacgtc aattagaaaa ccgtgcttca
     4141 ttccgtcgtg tacaaaaaca agcaatcact agagctatga aacttggtgc taaaggtatc
     4201 aaaactcaag tatctggtcg tttaggcgga gctgacatcg ctcgtgctga acaatattca
     4261 gaaggaactg ttccacttca tacgttacgt gctgacatcg attatgcaca cgctgaagct
     4321 gacactactt acggtaaatt aggcgttaaa gtatggatct atcgtggaga agttcttcct
     4381 actaagaaca ctagtggagg aggaaaataa taatgttact accaaaacgt gtaaaatatc
     4441 gtcgtcaaca tcgtcctaaa acaactggtc gttctaaagg cggtaactac gtaacatttg
     4501 gtgagtttgg tttacaagct acaacaacgt cttggatcac atctcgtcaa atcgaatctg
     4561 ctcgtatagc aatgacacgt tacatgaaac gtggcgggaa agtttggatt aaaatcttcc
     4621 cacatacacc atatactaaa aaacctttag aagtacgtat gggtgctggt aaaggtgcgg
     4681 ttgaaggctg gatcgcagtt gttaaaccag gtagaatttt attcgaagtt gctggcgttt
     4741 ctgaagaagt tgcgcgtgaa gcactacgtt tagcaagtca caaacttcca gtaaaaacta
     4801 agtttgtaaa acgtgaggaa ttgggtggtg aaacaaatga aagctaagga aattagagac
     4861 ttaaccactt cagaaatcga agaacaaatc aaatcttcaa aagaagagct ttttaaccta
     4921 cgctttcagt tagctacagg tcaattagaa gaaactgcac gtattcgtac agtaagaaaa
     4981 acgattgcac gtctaaaaac tgttgctcgt gaaagagaaa ttgaacaaag taaggctaat
     5041 caataattaa gtaagaggag gttacaaaag tgagcgaaag aaacgatcgt aaagtttatg
     5101 taggtaaagt tgtttcagac aaaatggaca agactattac agtacttgtt gaaacttaca
     5161 aaacacacaa attatacggt aaacgagtaa aatactctaa aaaatacaaa actcatgatg
     5221 aaaacaattc agctaaatta ggagacattg ttaaaattca agaaactcgt cctttatcag
     5281 caacaaaacg ttttcgttta gtagagattg ttgaagagtc agtaattatt taatacaagt
     5341 ttagagataa gggaggttta actaatgatc caacaagaaa cacgcttgaa agtagcagac
     5401 aactctggtg ctcgtgaagt tcttacaatc aaagtattag gtggatctgg tcgtaaaaca
     5461 gcaaacatcg gcgatgttat cgtatgtact gttaaaaatg caacaccagg tggcgttgtt
     5521 aaaaaaggtg acgttgtcaa agctgtaatc gtacgtacta agtcaggtgt tcgtcgtaat
     5581 gacggttcat acatcaaatt tgatgaaaat gcatgtgtta tcatccgtga tgacaaaggc
     5641 ccacgtggta ctcgtatctt cggacctgtt gctcgtgaat tacgtgaagg taacttcatg
     5701 aaaatcgtat cattagcacc agaagtactt taattaataa caaccaatac aaataaggag
     5761 gtgcccacat gcatatcaaa aaaggtgaca acgttaaagt tatcgcaggt aaagacaaag
     5821 gtaaagaagg taaagtaatt gctactctac ctaaaaaaga ccgtgtcgtt gtggaaggtg
     5881 ttaacattat gaaaaaacac caaaaaccaa ctcaattaaa tcctgaaggt ggaatcttag
     5941 aaacagaggc agcaatccat gtttctaatg tacaattatt ggaccctaaa acaaacgaac
     6001 caactcgtgt aggttacaaa tttgttgatg gtaaaaaagt tcgtatcgct aaaaaatctg
     6061 gcgaagaaat taaatctaat aattaataac aagttgaaag gaggatccca ctttgaaccg
     6121 tttaaaagaa aagtttaaca ctgaagttac tgaaaactta atgaaaaaat tcaattatag
     6181 ttcagtaatg gaagtaccaa aaatagataa aatcgttgtg aacatgggtg taggtgacgc
     6241 agtacaaaat tctaaagtat tagacaatgc tgttgaagaa ttagaattga tcactggtca
     6301 aaaaccatta gtaactaaag ctaaaaaatc aatcgcgact ttccgtttac gtgaaggtat
     6361 gccaatcggt gcgaaagtaa cacttcgcgg tgaaagaatg tatgaattct tagacaaatt
     6421 aatttcagta tcattaccac gtgtacgtga cttccaaggt gtttctaaaa aagcatttga
     6481 cggacgcggt aactacactt taggtgttaa agaacaatta attttcccag aaatcgacta
     6541 tgataaagta agtaaagtta gaggaatgga tattgttatc gtaacgactg ctaacactga
     6601 tgaagaagct cgtgaattgt tagctaactt cggtatgcca ttccgtaaat aattatttaa
     6661 aggaggctaa ttaagtggct aaaacttcaa tggttgctaa gcaacaaaaa aaacaaaaat
     6721 atgcagttcg tgaatacact cgttgtgaac gttgtggccg tccacattct gtatatcgta
     6781 aatttaaatt atgccgtatt tgtttccgtg aattagctta caaaggccaa atccctggcg
     6841 ttcgtaaagc tagctggtaa taaaaaagag tctgaaagga ggcaacaatc aatgacaatg
     6901 acagatccaa tcgcagatat gcttactcgt gtaagaaacg caaacatggt gcgtcacgag
     6961 aagttagaat tacctgcatc aaatattaaa aaagaaattg ctgaaatctt aaagagtgaa
     7021 ggtttcatta aaaatgttga atacgtagaa gatgataaac aaggtgtact tcgtttattc
     7081 ttaaaatatg gtcaaaacga tgagcgtgtt atcacaggat taaaacgtat ttcaaaacca
     7141 ggtttacgtg tttatgcaaa agctagcgaa atgcctaaag tattaaatgg tttaggtatt
     7201 gcattagtat caacttctga aggtgtaatc actgacaaag aagcaagaaa acgtaatgtt
     7261 ggtggagaaa ttatcgcata cgtttggtaa taaaaaataa ggaggtgcca taacatgagt
     7321 cgtgttggta agaaaattat tgacatccct agtgacgtaa cagtaacttt tgatggaaat
     7381 catgtaactg ttaaaggtcc taaaggtgaa ttatcaagaa ctttaaatga aagaatgaca
     7441 ttcaaacaag aagaaaacac aattgaagtt gtaagaccat ctgattctaa agaagataga
     7501 acaaaccatg gtacaactcg tgctttatta aacaatatgg tacaaggtgt ttctcaagga
     7561 tacgtaaaag tacttgaact tgttggtgta ggttaccgtg ctcaaatgca aggtaaagac
     7621 ttaatcctta acgttggtta ttctcaccca gtagaaatta aagctgaaga aaacattact
     7681 ttctcagttg agaaaaacac agtcgttaaa gttgaaggta tttcaaaaga acaagttgga
     7741 gcattagcat ctaacatccg ttcagtaaga cctccagagc cttacaaagg taaaggtatt
     7801 cgttaccaag gtgaatacgt tcgccgtaaa gaaggtaaaa ctggtaaata atagataaac
     7861 tctaaagaaa ggagtattga aatgatcagt aaaattgata aaaataaagt gcgtttaaaa
     7921 agacatgctc gtgttcgtac taacttatca ggtacagctg aaaagccacg tttaaacgta
     7981 tatcgttcaa acaagcatat ctacgctcaa attattgatg ataataaagg cgtaacatta
     8041 gctcaagctt cttcaaaaga cagcgacatt gctactacag caactaaagt tgaattagca
     8101 actaaagtcg gtgaagcaat tgctaaaaaa gctgctgaca aaggcattaa agaaatcgta
     8161 tttgaccgtg gaggatattt atatcacgga cgtgttaaag cattagctga agcagcaaga
     8221 gaaagcggat tagaatttta atttaaagga gggacaaata catggctcgt agagaagaag
     8281 agacgaaaga atttgaagaa cgcgttgtta caatcaaccg tgtagcaaaa gttgtaaaag
     8341 gtggtcgtcg tttccgtttc actgcattag ttgtagttgg agacaaaaat ggtcgtgtag
     8401 gtttcggtac tggtaaagct caagaggtac cagaagcaat caaaaaagct gttgaagcag
     8461 ctaaaaaaga tttagtagtt gttccacgtg ttgaaggtac aactccacac acaattactg
     8521 gccgttacgg ttcaggaagc gtatttatga aaccggctgc acctggtaca ggagttatcg
     8581 ctggtggtcc tgttcgtgcc gtacttgaat tagcaggtat cactgatatc ttaagtaaat
     8641 cattaggatc aaacacacca atcaacatgg ttcgtgctac aatcgatggt ttacaaaacc
     8701 ttaaaaatgc tgaagatgtt gcgaaattac gtggcaaaac agtagaagaa ttatacaatt
     8761 aaggagggaa aactagttat ggctaaatta caaattaccc tcactcgtag tgttattggt
     8821 cgtcctgaaa cacaacgtaa aactgttgaa gctttaggtc ttaaaaagac taacagttca
     8881 gtagttgttg aagataaccc tgctattcgt gggcaaatca acaaagttaa gcacttagta
     8941 acagtagaag aaaaataatc aaggaggtgc cgaaatgaaa ttacatgagt taaaaccggc
     9001 agaaggttca cgtaaagaac gcaatcgtgt tggacgtggt gttgcgacag gtaatggtaa
     9061 aacaagtggt cgcggacaca aaggtcaaaa agctcgttca ggcggtggtg taagaccagg
     9121 atttgaaggt ggtcaattac cattattccg tcgtttacca aaacgtggtt ttactaacat
     9181 aaatcgtaaa gaatatgcta ttgttaactt agaccaactt aataaatttg aagatggtac
     9241 tgaagtaact ccagctttat tagtagaatc tggtgttgtt aagaatgaaa aatctggtat
     9301 caaaatacta ggtaatggtt cacttgataa gaaattgaca gtgaaagctc ataaattctc
     9361 agcttcagca gcagaagcta ttgatgctaa aggtggagca cacgaggtga tctaatgatt
     9421 caaacccttg tgaacttctt tagaacaaaa gaggttcgta acaagatttt cttcacacta
     9481 gcaatgttag taatttttaa aatagggact tatataccag ctccaggagt aaatcctgca
     9541 gcttttgata atccccaagg ttctcaaggt gccactgagt tattaaatac ttttggtggc
     9601 ggagccttga aacgattttc tatttttgca atgggtattg taccctacat cactgcatca
     9661 atcgtaatgc aattattaca aatggatatt gtccctaaat tctcagaatg ggcaaaacaa
     9721 ggtgaagtag gtagaagaaa gttaaataac gttactcgtt atttagcaat ttctttagca
     9781 tttatccaat ctataggtat ggcattccaa tttaataatt atctcaaagg tgcgctgatt
     9841 atcaatcagt caattatgag ttatttatta atagcactag ttttgacagc aggaactgct
     9901 ttcttaatat ggcttggtga tcaaatcact cagttcggtg ttggtaatgg tatttctatt
     9961 atcatatttg cgggtatttt atcaacgttg ccagccagtc taattcaatt tggacaaact
    10021 gcgttcgttg ggcaagaaga tacatcatta gcatggttga aagtattagg acttttagtg
    10081 tcactgatac tattaactgt aggtgctatt tatgtacttg aagccgttcg taaaattccg
    10141 attcaatatg ctaagaaaca aactgctcaa agattaggtt cacaggcaac ttatctacct
    10201 ttaaaagtga actcagccgg tgttattcct gtaatctttg caatggcgtt cttcttactg
    10261 cctagaacat taacgttgtt ctatccagat aaagaatggg cacaaaacat tgcgaatgct
    10321 gcaaatcctt caagtaatgt tggtatggtt gtatatattg tactaattat actattcaca
    10381 tacttctatg catttgtaca agttaatccg gaaaaaatgg ctgataacct taagaaacaa
    10441 ggtagctatg ttccaggcat tagacctggt gaacaaacca aaaaatatat tactaaagtt
    10501 ttatatcgtt taacttttgt aggttctatc ttcttagccg ttatttcaat tctaccaata
    10561 ttagcaacta agtttatggg attaccacaa tcaattcaga ttggtggtac aagtttactt
    10621 atcgttattg gtgtagcgat tgaaacaatg aaatctttag aagctcaagt atctcaaaaa
    10681 gaatataaag gctttggtgg tagataattt taggagggca cttatgaata tcattttgat
    10741 gggtttacct ggcgcaggta aaggaactca agcaagtgaa attgtcaaga aattcccaat
    10801 accccacatt tcaactggtg acatgttcag aaaagctata aaagaagaaa ctgaattagg
    10861 taaagaagct aagtcttata tggaccgtgg cgaattagtt cctgatgaag tgactgtagg
    10921 tatcgttaag gaaagaattt ctgaagacga tgcaaaaaaa ggctttttat tagatggctt
    10981 cccaagaaca atcgagcaag ctgaggcatt aaataatatt atgtctgagc ttgacagaaa
    11041 cattgatgct gtcatcaata tcgaagttcc ggaagaagaa ttaatgaacc gtcttacagg
    11101 tcgtcgaatc tgtgagtcat gtggtacaac gtatcatctt gtatttaatc ctccgaaggt
    11161 cgaaggtatt tgtgatatcg atggtggtaa attgtatcaa cgagaagatg ataatcctga
    11221 aacggtagct aatcgtttga gtgttaatat taaacaatct aagcctattt tagatttcta
    11281 tgatcaaaaa ggtgtattga aaaatattga tggttcaaaa gatattagcg acgttaccaa
    11341 agatgtcatt gatattttag atcatttgta atagatcaac ttttagcggt ctattttaag
    11401 tgaggcatga tttaatcata ttagatagat gagagtggca attaacgata attaattccc
    11461 gtaatttttg tttaacttat aaaattaatc actgatagat tcgtaacaat aaagttgtct
    11521 attgacgatt accttactat tgcaaaaagg gggaagttaa tcaatggcta aacaagatgt
    11581 aattgaatta gaaggtactg tattagatac tttaccgaac gcaatgttta aagtagaatt
    11641 agaaaatggt catgagattt tagctcacgt aagtggtaaa atcagaatga attacattcg
    11701 tattctacct ggcgacaaag taactgttga gatgtctccg tacgatttaa cacgcggaag
    11761 aattacttat cgttataaat aatcgtcact ccataatata gggaggtata aaaatgaaag
    11821 taagaccatc agtaaaacct atttgcgaaa aatgtaaagt cattaaacgt aaaggtaaag
    11881 taatggtaat ttgtgaaaat ccaaaacaca aacaaagaca aggttaataa aagagaggtg
    11941 taaattaata tggcacgtat tgcaggagta gatattccac gtgaaaaacg cgtagttatc
    12001 tcattaactt atatatacgg tatcggtacg tcaactgctc aaaaaattct tgaagaagct
    12061 aacgtatcag ctgatactcg tgtgaaagat ttaactgatg acgaattagg tcgcatccgt
    12121 gaagttgtag acggttataa agtcgaaggt gacttacgtc gtgaaactaa cttaaatatc
    12181 aaacgtttaa tggaaatttc atcataccgt ggtatccgtc accgtcgtgg tttaccagtt
    12241 cgtggtcaaa aaacgaaaaa caacgcgcgt actcgtaaag gaccagttaa aacggtagct
    12301 aacaagaaaa aataataggt aaaggaggca aattttaaat ggcacgtaaa caagtatctc
    12361 gtaaacgtag agtgaaaaag aatattgaaa atggtgtagc acacatccgt tcaacattca
    12421 acaacactat tgtaactatc actgatgagt tcggtaatgc tttatcatgg tcatcagctg
    12481 gtgcattagg attcaaagga tctaaaaaat caacaccatt tgcagcacaa atggcttctg
    12541 aaactgcatc taaatcagct atggagcatg gtttaaaaac agttgaagta acagttaaag
    12601 gacctggtcc aggtcgtgaa tcagctattc gtgcattaca atctgcaggt ttagaagtaa
    12661 ctgcgatcag agacgttact ccagtacctc ataacggttg tcgtccacca aaacgtcgtc
    12721 gtgtataatt tatgatggta ttgttacagg tcactgagca aacattttaa attaagtcga
    12781 cgtatataag gaggatattt aaatgataga aatcgaaaaa cctagaattg agacaattga
    12841 aattagtgaa gatgctaaat tcggtaagtt cgttgttgaa ccactagaac gtggctacgg
    12901 tactacacta ggaaactcct tacgtcgtat cctactatct tcattaccag gtgcagccgt
    12961 taagtatatt gaaattgagg gagttttaca tgaattctca gcagtagaca atgtagttga
    13021 agatgtttct acaattatta tgaacattaa acaattagca ttgaaaattt actctgaaga
    13081 agataaaact ttagaaattg atgtacgtga tgaaggcgaa gtaacagcaa gcgacattac
    13141 acatgatagt gatgttgaaa ttttaaaccc agagcttaaa attgcaacag tatctaaagg
    13201 tggtcactta aaaattcgtc tagttgctaa caagggtaga ggttacgcat tagcagaaca
    13261 aaataatact agtgatttac caattggtgt aatccctgtt gattcattgt attcacctgt
    13321 tgaacgtgtg aactatactg ttgaaaatac acgtgtaggt caaagcagtg attttgataa
    13381 attaacattg gatgtttgga ctaatggttc aatcacacca caagaatcag tttcattagc
    13441 agcaaaaata atgactgaac acttgaatat cttcgttggt cttactgatg aagcgcaaaa
    13501 cgctgaaatc atgattgaaa aagaagaaga tcaaaaagaa aaagtattag aaatgtctat
    13561 tgaagaatta gacttatctg tacgttcata taactgctta aaacgcgcag gaatcaattc
    13621 tgttcaagag ttagctgaca aatctgaagc tgacatgatg aaagtgcgta atttaggtcg
    13681 taaatcttta gaagaagtta aatacaaatt agaagattta ggattaggat taagaaaaga
    13741 agattgataa aggaggttaa ctcatgggtt acagaaaatt aggtcgtact tctgatcaac
    13801 gtaaagctat gttacgtgac ttagctacat cacttattat tagtgaacgt attgaaacta
    13861 cagaagctcg tgcaaaagaa gttcgcagtg ttgttgagaa attaatcact ttaggtaaaa
    13921 aaggagattt agcttctcgt cgtaatgcag ctaaaacttt acgtaatgtt gaaatcttaa
    13981 acgaagatga aactacacaa actgcacttc aaaaattatt tggtgaaatc gcagagcgtt
    14041 acacagaacg tcaaggtggt tacactcgta tccttaaaca aggccctcgt cgcggtgacg
    14101 gtgctgaatc agtaattatc gaattagtat aagaatttat ataaatacac taactacttt
    14161 tatataaagc aaatgagtgc aacgataatg tttgccacat acaaatattg tctagctcag
    14221 agtaccccat caactaaata aagatttaaa gcgtgaactc aacaaactta tgatggggtg
    14281 cgcgcttttt tattgaataa gatatcggac ttatgaacag aaatgttttt aagtccgatt
    14341 tctttatatc tacttttttg agtgcgaatc taaaaatatg atcaagctag tttgatcaat
    14401 ttcactagaa ctgaatgaag tgaaagtatt atcgttgaac caaacgcgac cacccctata
    14461 ttagtaaatc atttatttta ttaacgttta tacataattg ttagagagga ataaatgtcc
    14521 ttatcattag ttgttaaaca acactttaaa gactagaaat gagttagaaa atactagtaa
    14581 tatatagcaa acccagtcaa tgtcatatac agcgatttat aaatattgta aaatatgata
    14641 gatacacgaa tcgtaaagga gagagacaag tggaggataa gaattcagtt attgtattta
    14701 aaaatgtttc atttcaatat caaagtgatg catccttcac attgaaagat gtttctttta
    14761 atatacctaa aggtcagtgg acatctattg ttggtcataa cggttctgga aaatctacaa
    14821 ttgccaagtt aatgattggc atagagaaag ttaaatctgg agaaattttt tataataatc
    14881 aagctataac tgatgataat tttgaaaagt taagaaaaga cataggaatt gtatttcaga
    14941 atccggataa tcaatttgtt ggttcaattg taaaatacga tgtggcattt ggactcgaaa
    15001 atcatgcggt tccacatgac gaaatgcata gaagagtcag cgaagcactt aaacaagttg
    15061 atatgttaga acgtgcagat tatgaaccta atgcattatc ggggggacag aagcagcgtg
    15121 tggctatagc aagtgtatta gcacttaacc cctctgtcat tatattagat gaggcgactt
    15181 ctatgttaga tcctgatgca cgtcaaaatt tattggattt agtgagaaaa gttaaatcag
    15241 aacataatat tacaatcatt tctattacgc atgatttatc tgaggcgatg gaagcagatc
    15301 atgttatcgt tatgaataaa gggactgtct ataaagaagg cacagcgact gaaattttcg
    15361 accatgcaga agagttaaca agaataggtc tagatttgcc attcccaatc aaaataaatc
    15421 aaatgctggg acaccaaaca tcattcttaa cttatgaagg gctggtggat caactatgac
    15481 tatacggttt gacaatgtaa gttataccta tcaaaaaggg acaccatatc agcatcaagc
    15541 tattcatgat gttaatacag aatttgaaca aggtaaatat tacgccatcg ttggacaaac
    15601 gggtagtggt aaatcaacgt tgatacaaaa tattaatgcg ctgttaaagc cgactactgg
    15661 gacagttaca gttgatgaca ttactattac acataagacc aaagataaat atattagacc
    15721 tgtaagaaaa agaattggaa tggtatttca atttcccgaa tctcaattat ttgaggacac
    15781 agtagagcgt gaaatgatat ttggacctaa aaactttaaa atgaatttag atgaagccaa
    15841 aaactatgcc catcgtctgt tgatggattt aggcttttca agagatgtaa tgtcacaatc
    15901 accatttcaa atgtcaggtg gacaaatgcg taaaatagcg attgtatcga tattggcaat
    15961 gaatcctgat attatcgtgg ttgatgaacc tacagcagga cttgatccac aaagtaaacg
    16021 acaagtaatg agattactaa agtcactaca aacagatgaa aataaggcaa ttatcctaat
    16081 ttcacatgat atgaatgaag tcgcgcgtta tgcagatgaa gtcatcgtta tgaaagaagg
    16141 tagtatagtg tcgcaaacat caccaaaaga gctcttcaaa gataaaaaaa aattggcgga
    16201 ttggcatatt ggtttgccag aaattgttca gttacaatat gactttgaac aaaaatatca
    16261 aacaaaatta aaagatattg ccttaactga agaagcattt gtaagcttgt ataaggagtg
    16321 gcaacatgaa aaataaattg attatagggc gatatttacc aattaattct tttgttcatc
    16381 atcttgatcc aagagcaaag ttaatgtttg tttttctttt tattatatta atatttttct
    16441 gtcattcacc attaacatat ctttgggtgt ttgcacttat cttattcatt atgagattag
    16501 caaaaattca attatggttc ctaatcaaag gtttaacacc aatatttttc tttttaatct
    16561 tcacattaat gatgcatata tttttaacta aaggtggata tgtattagtt gaatggcatg
    16621 gcatcacgat tgaaactaat ggtattttag aagggctata tatatcattg cgtttaattg
    16681 ggattgtgat gattgcaaca attatgacac tatctacaag tccaattgat ttaacagatg
    16741 cgtttgaaag attacttgca ccactaaaga tgtttaaatt accagttcat caattaagta
    16801 tgataatgtc cattgcgtta cgattcatcc cgacgttaat ggatgagtta gataaaatta
    16861 ttttggcgca aaagtcgcgt ggttctgaaa taagttcagg gaacattgca acacgtatta
    16921 aatcatttat tccattacta gtgccactat tcatctcagc tttccaacgc gccgaagaat
    16981 tagcggtcgc aatggaagtt aggggttatg atgccaatgt taagagaacg agctacagac
    17041 agcttaaatg gcaattgaga gatacgatat ctttaaccat gattatccca attgcaatta
    17101 ttttattcgt attaaaatat tcaggagtgt aaattcatgc gtatattagt agaaattgcg
    17161 tatcaaggaa ataattttct aggctttcaa attcaacaga atggacgtac ggtacagcaa
    17221 caatttgaaa agctattaca acgcatgcat aaaagacatg tgagaatcca tccttcaagt
    17281 aggactgata gaggtgtaca tgcgatacag cagtactttc atttcgacac agagctgaat
    17341 atacctatgt cacaatggca atatgcaatg aaccgaacgt taccagatga tatttatgtc
    17401 aataatgtag ttacagtcga tgacgatttt cattgtcgtt acgattgtgt cggtaaacgc
    17461 tatcgatata aagtatatca agcacaacac cgcgatccat ttcaaagtgg tttgaaaaca
    17521 tttattcctg aaacgttgga tttagacaaa atgaacagag cggcgcaaca attcataggt
    17581 acacatgatt ttacggggtt ttgttcgcaa aaaactgaag tagaaagcaa agttagaaca
    17641 ttataccaaa gtgaaatcgt aaagacagat gatggctttg attatattgt cactggatct
    17701 ggatttttat ataacatggt gcgtgtctta gttgcatttt taatagaagt tggaaaaggg
    17761 cgacatgaaa tttctgatgt gccgaagcta cttgaatcaa agaatcgtaa gaatgttcct
    17821 ttcactgcac cggctgaagg actatattta gagaagattt atttagatga aaatgagtta
    17881 ttaaaagact ttggcaacga tataaaaata catcgcaaaa aatcattaca aaatgactga
    17941 ttgccattga caaaaagctt gtgaaattat aagattatta acggtattgt tttatatcca
    18001 ccccacgata agccccggaa acttattgtg ttacaagata tataagcaga aacgaacaac
    18061 agttaacaaa ataaatgaaa ttaaacgttt taaaaatgaa acaaatgaaa tcatctatta
    18121 ggttatgaaa ctgtttatag cttgaataga agcatttatt ttttaggagg acaattatta
    18181 tgcgtcaaac atttatggca aatgaatcaa acattgagcg caaatggtat gttatcgatg
    18241 ctgaaggcca aacattaggt cgtttatcat cagaagtagc atctatctta cgcggtaaaa
    18301 ataaagtaac ttacacacca cacgttgata ctggtgatta tgtaatcgtt attaatgcat
    18361 caaaaatcga atttactggt aacaaagaaa ctgacaaagt ttactaccgt cactcaaatc
    18421 atccaggtgg tatcaaatca atcactgctg gtgaattaag aagaactaac ccagaacgtt
    18481 taattgaaaa ctcaattaaa ggtatgttac caagcactcg tttaggcgaa aaacaaggta
    18541 aaaaattatt tgtatatggt ggcgctgaac atccacacgc tgcacaacaa ccagaaaact
    18601 acgaattacg tggttaatta gaaggaggaa atgactttgg cacaagttga atatagaggc
    18661 acaggccgtc gtaaaaactc agtagcacgt gtacgtttag taccaggtga aggtaacatc
    18721 acagttaata accgtgacgt acgcgaatac ttaccattcg aatcattaat tttagactta
    18781 aaccaaccat ttgatgtaac tgaaactaaa ggtaactatg atgttttagt taacgttcat
    18841 ggtggtggtt tcactggaca agctcaagct atccgtcacg gaatcgctcg tgcattatta
    18901 gaagcagatc ctgaatacag aggttcttta aaacgcgctg gattacttac tcgtgaccca
    18961 cgtatgaaag aacgtaaaaa accaggtctt aaagcagctc gtcgttcacc tcaattctca
    19021 aaacgttaat tgtcggacga tatatacaaa acacctcgat attatgtcga ggtgtttttt
    19081 tgcgtttgtg tgttttaaaa ttatttggct ctatatcaaa ttggactgat gcattcaata
    19141 tcggaagtta ggcaactaaa cattgcttaa cttccttttt attttttgga gcgtaaagtt
    19201 ttgaacctaa attccaagag aattaataat ttttttcctt tctttttcga ggtcatcaaa
    19261 agtttgtata ttctttttaa ctatgttact tatatattca ataattatta atttaatcca
    19321 atttaatagc ttgtacacat cctcataatt atcatataaa tcaaatatac ttttaccatt
    19381 gtgaataatt tcagt
//
LOCUS       DAHHSC010000029        19050 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.029, whole genome shotgun sequence.
ACCESSION   DAHHSC010000029 DAHHSC010000000
VERSION     DAHHSC010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 19050)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19050)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19050
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.029"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            13..1707
                     /gene="ezrA"
                     /locus_tag="NBI57_000813"
     CDS             13..1707
                     /gene="ezrA"
                     /locus_tag="NBI57_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HCD1821778.1"
                     /translation="MVLYIILAIIVIILIAVGVLFYLRSNKRQIIEKAIERKNEIETL
                     PFDQNLAQLSKLNLKGETKTKYDAMKKDNVESTNKYLAPVEEKIHNAEALLDKFSFNA
                     SQSEIDDANELMDSYEQSYQQQLEDVNEIIALYKDNDELYDKCKVDYREMKRDVLANR
                     HQFGEAASLLETEIEKFEPRLEQYEVLKADGNYVQAHNHIAALNEQMKQLRSYMEEIP
                     ELIRETQKELPGQFQDLKYGCRDLKVEGYDLDHVKVDSTLQSLKTELSFVEPLISRLE
                     LEEANDKLANINDKLDDMYDLIEHEVKAKNDVEETKDIITDNLFKAKDMNYTLQTEIE
                     YVRENYYINESDAQSVRQFENEIQSLISVYDDILKEMSKSAVRYSEVQDNLQYLEDHV
                     TVINDKQEKLQNHLIQLREDEAEAEDNLLRVQSKKEEVYRRLLASNLTSVPERFIIMK
                     NEIDHEVRDVNEQFSERPIHVKQLKDKVSKIVIQMNTFEDEANDVLVNAVYAEKLIQY
                     GNRYRKDYSNVDKSLNEAERLFKNNRYKRAIEIAEQALESVEPGVTKHIEEEVIKQ"
     gene            complement(1781..1922)
                     /locus_tag="NBI57_000814"
                     /pseudo
     CDS             complement(1781..1922)
                     /locus_tag="NBI57_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500331.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2071..3219
                     /locus_tag="NBI57_000815"
     CDS             2071..3219
                     /locus_tag="NBI57_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HCD1821779.1"
                     /translation="MFILIYLDNAATTKAFEEVLDTYLKVNQSMYYNPNSPHKAGLQA
                     NQLLQQAKAQINAMINSKTNYDVVFTSGATESNNLALKGIAYRKFDTAKEIITSVLEH
                     PSVLEVVRYLEAHEGFKVKYVDVKKDGSINLEHFKELVSDKVGLVTCMYVNNVTGQIQ
                     PIPQMAKVIKNYPKAHFHVDAVQAFGKISMDLNNIDSISLSGHKFNGLKGQGVLLVNH
                     IQNVEPTVHGGGQEYGVRSGTVNLPNDIAMVKAMKIANENFEALNAFVTELNNDVRQF
                     LNKYHGVYINSSTSGSPFVLNISFPGVKGEVLVNAFSKYDIMISTTSACSSKRNKLNE
                     VLAAMGLSDKSIEGSIRLSFGATTTKEDIARFKEIFIIIYEEIKELLK"
     gene            3219..4442
                     /gene="thiI"
                     /locus_tag="NBI57_000816"
     CDS             3219..4442
                     /gene="thiI"
                     /locus_tag="NBI57_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HCD1821780.1"
                     /translation="MKYDHLLVRYGELTLKGSNRKKFVNQLRNNVNKSLKGLDGFVVK
                     GKRDRMYIELEDHADINEITYRLSKIFGIKSISPVLKVEKTIEAMSAAAIKFAQQFEE
                     NSTFKIDVKRADKNFPMDTYELQRELGGTVLKQIENVSVNVKRPDHEIRVEVRLDAIY
                     MYEEVVPGSGGLPVGTGGKTLLMLSGGIDSPVAGMEVMRRGVTIEAIHFHSPPFTSDQ
                     AKEKVIELTRILAERVGPIKLHIVPFTELQKQVNKVVHPRYTMTSTRRMMMRVADKLV
                     HQIGALAIVNGENLGQVASQTLHSMYAINNVTSTPVLRPLLTYDKEEIIIKSKEIGTF
                     ETSIQPFEDCCTIFTPKNPVTEPNFEKVVQYESVFDFEEMINRAVENIETLEITSDYK
                     TIKEQQTNQLINDFL"
     gene            4486..5256
                     /locus_tag="NBI57_000817"
     CDS             4486..5256
                     /locus_tag="NBI57_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HCD1821781.1"
                     /translation="MDLNLTMIIIIILFGFIAAFIDSVVGGGGLISTPALLAIGLPPS
                     VALGTNKLASSFGSLTSMIKFIRSGKVDLFVVAKLFGFVFLASACGAYIATMVPSQIL
                     KPLIIIALSSVFIFTLLKKDWGNTRTFTQFTFKKAIIFAALFILIGFYDGFVGGGTGS
                     FMLFVLLVFGFDFLSAAGNAKVLNFASNIGALVLFMVLGQVDYVIGLIMAISMIAGSY
                     TGAHFAIKQGVGYVKVLFIIITAILILKNAFDYIQQFV"
     gene            5354..5848
                     /gene="tpx"
                     /locus_tag="NBI57_000818"
     CDS             5354..5848
                     /gene="tpx"
                     /locus_tag="NBI57_000818"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HCD1821782.1"
                     /translation="MTEITFKGGPIHLKGQQINEGDFAPDFTVLDNDLNQVTLADYAG
                     KKKLISVVPSIDTGVCDQQTRKFNSEASKEEGIVLTISADLPFAQKRWCASAGLDNVI
                     TLSDHRDLSFGENYGVVMEELRLLARAVFVLDVDNKVVYKEIVSEGTDFPDFDAALAA
                     YKNI"
     gene            5971..6918
                     /locus_tag="NBI57_000819"
     CDS             5971..6918
                     /locus_tag="NBI57_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCD1821783.1"
                     /translation="MAEQQTIMERLFHTLDEKAKTLNNENGQSFIENLGLAMEQVYTN
                     ERGLLEQSTLQDRRKAFQFAYLSLMQEEKIQANHQITPDSIGLILGFLVERFMNNQEE
                     LHIVDIASGAGHLSATVKEVLPEIAVMHHLIEVDPVLSRVSVHLANFLEIPFDVYPQD
                     AIMPLPLEEADIVIGDFPVGYYPIDERSKEFKLGFEEGHSYSHYLLIEQAINALKDAG
                     YAFLVVPSNIFTGEHVKQLEKYIATETEMQAFLNLPPTLFKNEKARKSILILQKKKSG
                     ETKPVEVLLANIPDFKNPSQFQGFMTELNQWMDTNRPKK"
     gene            7006..8208
                     /locus_tag="NBI57_000820"
     CDS             7006..8208
                     /locus_tag="NBI57_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HCD1821784.1"
                     /translation="MSKLILAINAGSSSLKFQLIRMPEEELVTKGLIERIGLKDSIFT
                     IEVNGEKVKTVQDIKDHVEAVDIMLDAFKAHNIINDINDIDGTGHRVVHGGEKFPESV
                     AITDEVEKEIEELSELAPLHNPANLMGIRAFRKLLPNIPHVAIFDTAFHQTMPEKAYL
                     YSLPYHYYKDYGIRKYGFHGTSHKFVSQRAAEMLDKPIEDLRIISCHIGNGASIAAID
                     GGKSIDTSMGFTPLAGVTMGTRSGNIDPALIPFIMEKTGKTAEQVLEILNKESGLLGL
                     SGTSSDLRDLSEEAESGKARSQMALDVFASKIHKYIGSYAARMHGVDVIVFTAGIGEN
                     SVEIRAKVLEGLEFMGVYWDPKKNENLLRGKEGFINYPHSPVKVVVIPTDEESMIARD
                     VMTFGGLK"
     gene            complement(8454..8954)
                     /locus_tag="NBI57_000821"
     CDS             complement(8454..8954)
                     /locus_tag="NBI57_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HCD1821785.1"
                     /translation="MITYKNILIAVDGSHEAEWAFNRAVGVAKRNDAKLTIVNVIDSR
                     TYSSYEVYDAQFTEKSKHFAEELLNGYKEVATNAGVKDVETRLEFGSPKSIIPKKLAH
                     EINADLIMSGTSGLNAVERFIVGSVSESIVRHAPCDVLVVRTEELPADFQPQVATTQL
                     REKYQN"
     gene            9095..10213
                     /gene="ald"
                     /locus_tag="NBI57_000822"
     CDS             9095..10213
                     /gene="ald"
                     /locus_tag="NBI57_000822"
                     /EC_number="1.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HCD1821786.1"
                     /translation="MKIGIPREIKNNENRVGLSPSGVHALVESGHTVLVETNAGSGSF
                     FEDVDYKEAGAEIVAEQAKVWDVDMVIKVKEPLESEYPYFKEGLVLFTYLHLANEEKL
                     TQALIDRKVISIAYETVQLPDRSLPLLSPMSEVAGRMSAQVGAEFLQKLNGGMGILLG
                     GVPGVPKGKVTIIGGGQAGTNAAKIALGLGADVTILDVNPKRLQQLDDLFGGRVHTIM
                     SNPLNIELYVKQSDLVIGAVLIPGAKAPRLVTEDMIKQMKNGSVIIDIAIDQGGIFET
                     TDKITTHDDPTYIKHGVVHYAVANMPGAVPRTSTLALNNATLPYALMLANKGYREAFK
                     SNQPLSLGLNTYKGHVTNKGVAEAFEMEYKSVEEALQL"
     gene            10337..10435
                     /locus_tag="NBI57_000823"
     CDS             10337..10435
                     /locus_tag="NBI57_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1821787.1"
                     /translation="MNDIDLIHYMLLMNIPAYVFLACLLHIAFRLG"
     gene            complement(10529..11584)
                     /locus_tag="NBI57_000824"
     CDS             complement(10529..11584)
                     /locus_tag="NBI57_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HCD1821788.1"
                     /translation="MTKISKIIDELNNQQADAAWITTPLNVYYFTGYRSEPHERLFAL
                     LIKKDGKQVLFCPKMEVEEVKASPFTGEIVGYLDTENPFSLYPQTINKLLIESEHLTV
                     ARQKQLISGFNVNSFGDVDLTIKQLRNIKSEDEISKIRKAAELADKCIEIGVSYLKEG
                     VTEREVVNHIEQTIKQYGVNEMSFDTMVLFGDHAASPHGTPGDRRLKSNEYVLFDLGV
                     IYEHYCSDMTRTIKFGEPSKEAQEIYNIVLEAETSAIQAIKPGIPLKDIDHIARNIIS
                     EKGYGEYFPHRLGHGLGLQEHEYQDVSSTNSNLLEAGMVITIEPGIYVPGVAGVRIED
                     DILVTNEGYEVLTHYEK"
     gene            11759..12448
                     /locus_tag="NBI57_000825"
     CDS             11759..12448
                     /locus_tag="NBI57_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HCD1821789.1"
                     /translation="MKLSFHGQSTIYLEGNNKKVIVDPFISNNPKCDLNIETVQVDYI
                     VLTHGHFDHFGDVVELAKKTGATVIGSAEMADYLSSYHGVENVHGMNIGGKANFDFGS
                     VKFVQAFHSSSFTHENGIPVYLGMPMGIVFEVEGKTIYHTGDTGLFSDMSLIAKRHPV
                     DVCFVPIGDNFTMGIDDASYAINEFIKPKISVPIHYDTFPLIEQDPQQFKDAVNVGDV
                     QILKPGESVQF"
     gene            complement(12753..13166)
                     /locus_tag="NBI57_000826"
     CDS             complement(12753..13166)
                     /locus_tag="NBI57_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HCD1821790.1"
                     /translation="MYKNILLGVDTQLKNEKALKEVSKLAGEGTVVTVLNAISEQDAQ
                     ASIKAGVHLNKLTEERSKRLEKTRKALEDYGIDYDQIIVRGNAKEELLKHANSGKYEI
                     VVLSNRKAEDKKKFVLGSVSHKVAKRATIPVLIVK"
     gene            13371..14669
                     /locus_tag="NBI57_000827"
     CDS             13371..14669
                     /locus_tag="NBI57_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HCD1821791.1"
                     /translation="MTKHEQILDYIESLSIGSKISVRKIAKFLNVSEGTAYRAIKDAD
                     KMGMVATIDRVGTVRIEKRNRNEIEHLTFNEIVNIIDGQVLGGNKGITKMVSKFAIGA
                     MELKDILKYIGPKTLLIVGNREDVQIEALKRGTAILITGGFKPSNKVINFANEHDLPV
                     LSSSYDTFLVANIINKALFNQKIRKDILIVQDIMTPLDDLSVLFDTMKIADYKRMANR
                     TGHTRFPVVNESYKLVGIVTSREMINTKDDDEIDKVMTRNPIYVNAMSTVASCAHMMI
                     WEGIELIPVVSSNKKTVGVINRQDVLKSMQLLGRQPQMGETINDQIAKYITMNQDGIT
                     VEVSPLLINHYGTVSKAAFVSIIEETIQYEMRKFKKGNVMIENLNIVYIKTVPIESHI
                     TVRFGILDVGRNFAKIEVNMHSQNDKVASALVICQMFDEV"
     gene            14981..15922
                     /gene="pde2"
                     /locus_tag="NBI57_000828"
     CDS             14981..15922
                     /gene="pde2"
                     /locus_tag="NBI57_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pApA hydrolase Pde2"
                     /protein_id="HCD1821792.1"
                     /translation="MISTLNEIMKCIEDNDTIIIHRHVRPDPDAYGSQLGLKYYIQQK
                     FPQKQVFAVGEAESSLSFIGELDNIDDKTYQDALVIVCDTANAPRIDDERYSTGSKLI
                     KIDHHPAVDQYGDINLVNTNASSTSEIIYDLISHFNDEAIVNKDIASVLYLGIVGDTG
                     RFLFNNTSEHTMEIAGKLIGHDIDHNALLNKMMEKDPKMLPFQGYVLQHFELMDDGFC
                     QVKITEDVLEQFGIQPNEASQFVNTIADIKGLKIWVFAVDEGSEIRCRLRSKGQLIIN
                     DIAQDFGGGGHPNASGVSVNSWDEFEQLATALRTKLN"
     gene            15943..>19050
                     /locus_tag="NBI57_000829"
     CDS             15943..>19050
                     /locus_tag="NBI57_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HCD1821793.1"
                     /translation="MVAYLNIHTAYDLLNSSLKIEDAVRLAVSENVDALAITDTNVLY
                     GFPKFYDACIANNIKPIFGMTIYVTNGLNTVETVVLAKNNDGLKDLYQLSSEIKMNSM
                     ENVSFELLQQFSSNLIIIFKNVADEHRDIVQVFDSHEDTYLDHQSVLVQGIKHVWIQN
                     VCYQTRQDADTISALAAIRDNAKLDLIHDQEDFGAHFLTEKEIKQLDINQEYLTQVDV
                     IAQKCNAELKYHQSLLPQYQTPNDESAKKYLWRVLVTQLKKLELNYDVYLERLKYEYK
                     VITNMGFEDYFLIVSDLIHYAKTNDVMVGPGRGSSAGSLVSYLLGITTIDPIKFNLLF
                     ERFLNPERVTMPDIDIDFEDTRRERVIQYVQEKYGELHVSGIVTFGHLLARAVARDVG
                     RIMGFDEVTLNEISSLIPHKLGITLDEAYQIDDFKKFVHRNHRHERWFSICKKLEGLP
                     RHTSTHAAGIIINDHPLYEYAPLTKGDTGLLTQWTMTEAERIGLLKIDFLGLRNLSII
                     HQILIQVKKDLGINIDIEKIPFDDQKVFELLSQGDTTGIFQLESDGVRSVLKKLKPEH
                     FEDIVAVTSLYRPGPMEEIPTYITRRHDPSKVQYLHPHLEPILKNTYGVIIYQEQIMQ
                     IASTFANFSYGEADILRRAMSKKNRAVLESERQHFIEGAKQNGYHEDISKQIFDLILK
                     FADYGFPRAHAVSYSKIAYIMSFLKVHYPNYFYANILSNVIGSEKKTAQMIEEAKKQG
                     ITILPPNINESHWFYKPSQEGIYLSIGTIKGVGYQSVKVIVDERYQNGKFKDFFDFAR
                     RIPKRVKTRKLLEALILVGAFDAFGKTRSTLLQAIDQVLDGDLNIEQDGFLFDILTPK
                     QMYEDKEELPDALISQYEKEYLGFYVSQHPVDKKFVAKQYLTIFKLSNAQNYKPILVQ
                     FDKVKQIRTKNGQNMAFVTLNDGIETLDGVIFPNQFKKYEELLSHNDLFIVSGKFDHR
                     KQQRQLIINEIQTLATFEEQKLAFAKQIIIRNKSQIDMFEEMIKATKENANDVVLSFY
                     DETIKQ"
BASE COUNT         6695 a         2668 c         3544 g         6143 t
ORIGIN      
        1 gaggagaagc atatggtgtt atatatcatt ttggcaataa ttgtgattat attgattgct
       61 gtaggtgtat tattctattt acgttcaaat aaacgacaaa ttattgaaaa agcaatcgaa
      121 cgtaaaaatg aaattgagac gttacctttt gatcaaaacc ttgcacaatt atctaagttg
      181 aatttaaaag gtgaaacaaa aacgaaatac gatgcaatga aaaaggacaa cgtagaaagt
      241 acaaataagt atctagctcc tgtggaagaa aaaatccata atgctgaggc tttattagat
      301 aaatttagtt tcaacgcatc tcaaagtgaa attgatgatg caaatgagtt gatggatagt
      361 tacgaacaaa gctatcagca acaattagaa gatgtaaatg aaattattgc gttatacaaa
      421 gataatgatg aattatatga caaatgtaag gttgattatc gtgaaatgaa acgtgatgtt
      481 ttagcaaatc gtcatcaatt tggtgaggca gcaagtctac ttgaaactga aattgaaaag
      541 tttgagccaa ggttagagca atatgaagta ctaaaagctg atggtaatta tgtacaagcg
      601 cacaaccata tagctgcctt gaatgaacaa atgaaacagc taagatctta tatggaagaa
      661 ataccagaat taattagaga aactcaaaaa gaattacctg gtcaattcca agatttaaaa
      721 tatggttgcc gtgatcttaa agttgaaggg tatgatctgg atcacgtaaa agtagacagt
      781 acattacaaa gcttaaaaac agagcttagt ttcgttgaac cattaattag ccgcttagaa
      841 ttagaagaag ctaatgataa actagctaat atcaatgata agttagatga catgtatgat
      901 ttaattgaac atgaagttaa agctaaaaat gatgtcgaag aaacaaaaga tatcattacg
      961 gataacttat tcaaagctaa agacatgaat tatacattgc aaacagaaat tgaatatgta
     1021 cgtgaaaact actatataaa tgaatctgat gctcaaagtg ttcgtcaatt tgaaaatgaa
     1081 attcaaagtt taatttctgt atatgatgat attttaaaag aaatgtctaa atctgctgta
     1141 cgatatagcg aggttcagga taatttacaa tatttagaag atcatgtcac agttattaat
     1201 gacaaacaag aaaagctaca aaatcatctg attcaattgc gtgaagatga agcagaagca
     1261 gaagacaatc tgctacgagt acaatcgaag aaagaagaag tgtatcgtcg attacttgct
     1321 tctaacttaa caagcgttcc tgaaaggttt atcatcatga aaaatgaaat tgatcatgaa
     1381 gttcgtgatg ttaacgaaca atttagtgaa cgtccaatac acgttaaaca gttaaaagat
     1441 aaagtgtcta aaattgtgat tcaaatgaat acatttgaag atgaagcaaa tgatgttctt
     1501 gttaatgctg tttatgcaga gaaattaatt caatatggaa atagatatcg taaggactat
     1561 agcaatgttg ataagagctt aaatgaagct gaacgattat ttaaaaataa tcgctataag
     1621 cgtgcgattg aaattgcaga gcaagctctt gaaagtgttg agccaggtgt cactaaacat
     1681 attgaagaag aagttattaa gcaatagaaa ctagtatgta gttatactta aataatatga
     1741 gcactctgtc aaattggact gatgagttta ataattgaag ttagccaacg atacgttgtc
     1801 tagcttcttt ttttatatgg ataaatgaaa gggacaataa atataaatag caattgttta
     1861 aagataaacg taatcaaatg tgttgtttta attaatataa gtagtgaaaa aagcataatc
     1921 acacagctgt ttaaatagag tgaaatagtc taattcttat ttaataagta gaaataagat
     1981 tattttgcgc tataaccttt attttttgtc ggataatagt ataatgcttg agtaaaacaa
     2041 ttttaacgat tgatgattaa tgaaagagaa gtgtttattt tgatatatct agataatgcg
     2101 gcaacgacga aagcatttga agaagtgtta gatacttatt taaaagtaaa tcaatcaatg
     2161 tattataatc cgaatagtcc gcataaagct ggtttgcagg caaatcaatt actacaacaa
     2221 gcaaaagccc aaattaatgc aatgattaat tcaaaaacaa attatgatgt tgtattcact
     2281 agtggtgcaa ctgaatccaa taatcttgct ttaaaaggta ttgcctatcg taaatttgat
     2341 acagcgaagg aaataattac atccgtgtta gagcatccgt ccgtattaga ggttgtaaga
     2401 tatttggaag cacacgaagg atttaaagtt aaatatgttg atgtaaagaa agatggcagt
     2461 attaacttag aacacttcaa agaattagtg tcagacaaag tcggtttagt aacatgtatg
     2521 tatgtaaata atgtaactgg acaaatacag cctattccac aaatggctaa agttataaaa
     2581 aattatccta aggcacattt tcatgtagat gcggttcaag cattcggcaa aatttcaatg
     2641 gatctcaata acatagatag tattagttta agtggacaca agtttaatgg tttaaaagga
     2701 caaggcgtct tacttgtaaa tcacattcaa aatgttgaac caactgtcca tggtggtggt
     2761 caagaatatg gcgttagaag tggaacagtt aatttgccaa atgatattgc aatggttaaa
     2821 gcgatgaaga tagctaatga aaactttgaa gcattgaatg catttgttac tgagttaaat
     2881 aatgacgtcc gtcaattttt aaataaatat catggagttt atattaattc ttcaacttca
     2941 ggttcaccat tcgttttaaa tattagtttt cctggcgtaa aaggtgaagt attagttaat
     3001 gctttttcaa aatatgacat tatgatatct acgacgagtg cttgttcatc taaacgtaat
     3061 aaattaaatg aagtattggc tgcaatggga ttatcagaca aatctattga aggtagtata
     3121 agattatcat ttggggctac tacaactaaa gaagatatag caaggtttaa agaaatattt
     3181 atcatcattt atgaggaaat taaggagttg ctaaaataat gaagtatgat cacttgcttg
     3241 ttagatacgg ggagttaaca ttaaagggtt caaatagaaa aaaatttgta aatcaattaa
     3301 gaaataatgt aaataagtca ttaaaaggac ttgatgggtt tgtcgttaaa ggcaaacgag
     3361 atcgtatgta tattgaactt gaagaccatg cagatataaa tgaaataaca tatcgattat
     3421 caaaaatttt cggtattaaa tctattagtc cagtattaaa agtagaaaaa acaatagagg
     3481 caatgagtgc agcggcaatt aaatttgcgc agcaatttga agaaaacagc acatttaaaa
     3541 ttgatgtgaa gcgtgccgat aaaaatttcc caatggatac gtatgaatta caacgtgaat
     3601 tgggtggtac cgtattgaaa caaattgaaa atgtttctgt taatgtcaaa cgtccagatc
     3661 atgaaattcg agtggaagtt agattagatg caatttatat gtatgaagaa gttgttccgg
     3721 gttcaggcgg attaccagtt ggtactggtg gtaagacgtt actaatgctt tcaggcggta
     3781 tagactcacc agttgctgga atggaagtga tgagacgtgg cgtaacaatt gaagcgattc
     3841 atttccatag tccaccattt acaagtgatc aagcaaaaga aaaagttatt gaattgacac
     3901 gtattttagc tgaacgtgtt ggaccaatta aattgcatat tgtaccattt acagaattgc
     3961 aaaaacaggt aaataaagtt gtacatccaa gatatacaat gacttcaacg agacgtatga
     4021 tgatgcgtgt tgctgataaa ttagtacatc aaataggggc tttagctatt gtaaatggtg
     4081 aaaacctagg gcaggtagcc agtcaaacac ttcatagcat gtatgcaatt aataatgtaa
     4141 cttctactcc tgtattacgt cctttattaa cttacgataa agaagaaatt attattaaat
     4201 cgaaagaaat tggtacattt gaaacatcta ttcaaccatt tgaagattgt tgtacaattt
     4261 tcacccctaa aaatccagta accgaaccaa actttgaaaa ggtagtccaa tatgaaagtg
     4321 tctttgattt tgaagagatg attaatcgtg ctgttgaaaa tattgaaaca cttgaaataa
     4381 ctagtgatta taaaactatt aaagaacagc aaacaaacca attaataaat gactttttat
     4441 aaataaaatc ctagagtaaa tttaaacata aggggatgtt aaactatgga tttgaactta
     4501 acgatgatta taatcataat tttatttggt tttatcgcgg cgtttataga ttcggttgta
     4561 gggggtggcg gtttaatttc tacgccagca ttattagcaa tcggtctacc accatctgtg
     4621 gctttaggta caaataaatt ggcaagttca tttggttctt taactagtat gataaagttt
     4681 ataaggtccg gtaaagtgga cttatttgtt gttgccaaat tatttggttt tgtatttttg
     4741 gcatctgcat gtggcgcata tattgcaacg atggttccgt cacaaatatt gaaacctcta
     4801 atcattattg cactttcgtc ggtgtttata ttcacattac ttaaaaaaga ttggggcaat
     4861 acacgcacgt ttactcaatt tacatttaag aaagccataa tatttgcagc actttttata
     4921 ttaatcggct tttatgatgg atttgtaggt gggggaacag gttcatttat gctttttgta
     4981 ttgttagtct ttggttttga ttttttaagt gcagcaggaa atgctaaagt tttgaacttt
     5041 gcttctaata taggtgcgct tgtattattt atggtattag gacaagtaga ttatgtaata
     5101 ggtttaatta tggctataag tatgattgct gggtcatata ctggagcaca ttttgctatc
     5161 aaacaaggtg ttggctatgt aaaagtatta tttattatta ttactgctat tttaatttta
     5221 aaaaatgctt ttgattatat tcaacaattt gtataaaatt aaatttgctt ttgattaaag
     5281 catgaaaatt gtaatcaaac cataaattgt cgtatgatgt agttagaatt ttaaaatgca
     5341 ggaggtcaag tatatgactg aaataacatt caaaggtgga ccaatccact taaaaggtca
     5401 acaaattaat gaaggtgatt ttgcacctga ttttacagtg ttagataatg acttaaatca
     5461 agtaacatta gcagattatg ctggtaaaaa gaaattaatt agtgtggtac catcaattga
     5521 tacaggtgtt tgtgatcagc agactcgcaa attcaactct gaagcttcta aagaagaggg
     5581 gattgtgctt acaatttcag cagacttacc attcgcacaa aaaagatggt gcgcttcagc
     5641 aggtttagac aatgtcatta cattaagtga ccaccgtgac ttatcatttg gtgaaaacta
     5701 tggcgttgtt atggaagaac ttcgcttatt agctcgtgca gtatttgtat tagatgtaga
     5761 taataaagtt gtttataaag aaatcgttag tgaaggtact gatttcccag attttgatgc
     5821 tgctttagct gcatacaaaa atatttaatc attaaagaga taaatcttaa aatgtataca
     5881 tcgtgtccat cgttgtcaac agcattaaaa tagaattgtt ttctatgatt gctaagacct
     5941 atgggcactt tttattggag agggacgaat atggcagaac aacaaacaat tatggaacgc
     6001 ttgtttcata cattagatga aaaagctaaa acattaaata atgaaaatgg ccaaagtttt
     6061 attgaaaatc ttgggctagc aatggaacaa gtatatacca atgaaagagg attgttagaa
     6121 caatcgacgt tacaagatcg acgtaaagca ttccaatttg catatttaag tttaatgcag
     6181 gaagaaaaga tacaagcaaa tcatcaaatt acaccagatt caattggatt gatactagga
     6241 tttttagttg agcgttttat gaacaaccaa gaagaattac atattgttga tattgcaagt
     6301 ggtgccggtc atttaagtgc tactgtaaaa gaagtgttac ctgaaattgc ggttatgcat
     6361 catttaattg aagttgatcc agttttatca cgtgttagtg tacatttagc aaacttctta
     6421 gaaattcctt tcgatgtgta tcctcaagat gccatcatgc cactaccatt agaagaagca
     6481 gatatcgtta ttggtgattt tccagtaggc tattatccaa ttgatgaaag aagtaaggag
     6541 tttaagctag gttttgaaga aggacatagt tattcacatt atttattaat agaacaagca
     6601 ataaatgcat taaaagatgc tggatatgcc tttctagtgg taccaagtaa tatttttaca
     6661 ggtgaacatg taaaacagct tgaaaaatat attgcaacag agacagagat gcaagcattt
     6721 ttaaatttac caccaacttt atttaaaaat gaaaaagcgc gaaaatctat attaatttta
     6781 caaaagaaaa aatcgggtga aacaaagcca gttgaagtat tattggcaaa tattcctgat
     6841 ttcaaaaatc cttcacaatt tcaaggattt atgacagagt taaatcagtg gatggacaca
     6901 aatcgtccta aaaaataatc tgtaatagta tagtcataaa actgtatgaa tgataaaatg
     6961 agaaatgaaa tagtataaaa agcaaacgat ggaggatcat tacttatgtc aaaattaatc
     7021 ttggctatca atgctggtag ttcatcatta aaatttcaat taattagaat gcctgaagag
     7081 gaattagtaa caaaaggttt aatagaaaga atcggattga aggattcaat ttttacaatt
     7141 gaagtcaacg gggaaaaagt taaaacagta caagatatca aggatcacgt tgaggcagtt
     7201 gatatcatgt tggatgcttt taaagcgcat aatattatta atgatattaa cgatatcgat
     7261 ggaacaggtc accgtgttgt acatggtggt gaaaaattcc ctgaatcagt agctattact
     7321 gatgaggttg aaaaggaaat tgaagaatta agcgaattag caccgcttca caacccagct
     7381 aacttgatgg gtattcgtgc tttccgtaag ttattaccaa atatccctca tgtggcaatt
     7441 tttgatacag cattccatca aacaatgcct gaaaaagcat atctatatag cttgccatat
     7501 cattattata aagattatgg cattcgtaag tatggtttcc atggtacaag ccataaattt
     7561 gtatcacaaa gagcggcaga aatgttagat aagccaattg aagatttacg tattatttca
     7621 tgtcacattg gtaatggagc atcaattgca gctatcgatg gcggtaaatc tattgataca
     7681 tcaatgggct tcacaccact tgctggtgta acaatgggta ctcgttctgg taatattgac
     7741 cctgctttaa ttccatttat tatggaaaaa acaggtaaaa ctgctgaaca agtattggaa
     7801 atcttaaaca aagaatcagg tttattagga ttatcaggta catctagtga tttacgtgac
     7861 ttatcagaag aagctgaatc aggtaaagca cgttcacaaa tggctttaga tgtatttgct
     7921 tctaaaattc acaaatatat tggatcatat gctgcgagaa tgcatggtgt tgatgttatc
     7981 gtatttacag caggtatcgg tgaaaactca gtagaaattc gtgcgaaagt tcttgaaggc
     8041 ttagaattta tgggtgttta ctgggatcct aagaaaaatg aaaacttact acgtggtaaa
     8101 gaaggattta ttaactatcc tcattcacca gttaaagttg tcgttattcc aacagatgaa
     8161 gaaagtatga ttgcccgtga tgttatgaca ttcggtggtt taaaataatt tttaaataaa
     8221 tattaagcgt ctatctccgc tacaattata tgtaggaggt agacgctttt tgtattgttt
     8281 gaaaattaac tagtgtaatg tctcttttca cgaatatctt aaagaattaa gtagtcgaag
     8341 aagattatga aaacattatt aagtgagttt tatttataaa caataaaaaa ccaagtgcag
     8401 aaagttgcac ttggttcata atgatcaagt agttatctaa tgatcatcta tatttaattt
     8461 tgatattttt cacgtaattg agttgttgca acttgtggtt ggaagtctgc tggtaactct
     8521 tcagtacgaa caactaacac gtcacatggc gcatgacgaa cgatagattc tgatacagaa
     8581 ccaacaataa atctttccac ggcatttaag cctgatgtac cactcataat caagtctgca
     8641 ttaatttcat gtgcaagctt tttaggaatg atagatttag gagagccaaa ctctagacgc
     8701 gtttctacat ctttaacacc agcgttagta gctacttctt tataaccatt taataattct
     8761 tctgcaaaat gcttagattt ttcagtaaat tgagcatcat aaacttcata agaagaatac
     8821 gttcttgaat caattacatt cacaattgtt aacttcgcat cgttacgttt agcaacacca
     8881 actgctctgt taaatgccca ttccgcttca tgtgaaccgt caactgcgat taaaatattt
     8941 ttgtaagtaa tcatagtaag tgcctccttg ttctttctta attatatttt accacaattt
     9001 tcggaaaagt cagtgtaata acctttacaa ttttgtggat ttttgattgc gctttcaaaa
     9061 tgttcgcgtt aagatgatta tggaggtgga tggtatgaaa attggtatac caagggagat
     9121 taaaaataat gaaaatcgtg ttggtttatc accgagtggt gtgcacgctt tagtagaaag
     9181 tgggcatact gttttagtgg aaacaaatgc gggttcagga tcgttctttg aagatgtaga
     9241 ttacaaagaa gcaggtgctg agattgttgc tgaacaagca aaagtttggg atgtggatat
     9301 ggttattaaa gttaaagaac cacttgaatc tgaatatcca tattttaaag aagggcttgt
     9361 attattcact tatcttcatt tagcaaatga agaaaaatta acacaagctt tgatagatag
     9421 aaaagtaatt agtattgcat atgagactgt gcagttacca gaccgatctt taccattgtt
     9481 atcaccaatg agtgaggtag caggaagaat gtcagctcaa gttggcgcag agttcctaca
     9541 aaaacttaat ggtggtatgg gaattctact aggtggtgtc ccaggagtac ctaagggtaa
     9601 agtaactatt atcggtggtg gtcaagcagg aacaaatgca gctaaaattg cactaggact
     9661 aggtgcagat gttacgattt tagatgttaa tccaaagcgt ttacaacaat tagatgattt
     9721 attcggtgga cgtgtacata caattatgtc aaatccgttg aatattgagt tgtatgttaa
     9781 acaaagtgat ttagtaatag gtgcagtttt aattccaggt gctaaagcgc caagacttgt
     9841 aacagaagac atgattaaac aaatgaaaaa tgggtcagtt attattgaca ttgctattga
     9901 tcaaggcggt atttttgaaa caactgataa aattacgaca catgatgatc ctacatatat
     9961 taagcatggt gtggttcatt atgcagttgc aaatatgcca ggtgcagtac cgcgtacttc
    10021 gacgttagct ttaaataatg ctacgctacc ttatgcgctc atgctagcta ataaagggta
    10081 tagagaagca tttaaatcaa atcaaccatt atcattaggt ttaaatactt acaaaggtca
    10141 cgtaaccaat aaaggcgttg cagaggcatt tgaaatggaa tataaatctg tagaagaagc
    10201 attacaatta taatttgaat ttgatgaaaa gggaatggat tacagcgtac taaagaatga
    10261 cgttatcatc tccttggtta ttaaaattaa ataatgataa attaaagcta gaaacaatgc
    10321 gaagaaatca tcgaacatga atgatattga tttgattcat tacatgttac tgatgaacat
    10381 acctgcatat gtgtttctag cttgtttgtt acatattgca tttagacttg gataaaaatt
    10441 tatgtcttag gcgctacaat gttatattgg cagtgtttga ctgaatgaga atgtgcttgt
    10501 aacaagcttt tcatttttat cccactcctt atttttcgta atgtgttaat acttcatatc
    10561 cttcattagt gacaagtatg tcatcttcaa ttcttacacc tgcaacacct ggtacataaa
    10621 tacctggttc gattgtaata accatgccag cttctaacaa attagaatta gtacttgaaa
    10681 catcttgata ttcatgttct tgtaatccta aaccatgacc taagcgatga gggaaatatt
    10741 caccataacc tttttctgaa ataatatttc tagcgatatg atcgatatct tttaaaggta
    10801 ttccaggttt aattgcttgg attgcagatg tttctgcttc taatacaata ttataaattt
    10861 cttgtgcttc tttgctaggt tcaccaaatt taatggtacg tgtcatatcg ctacaataat
    10921 gctcataaat tacacctaaa tcaaatagta catattcatt gctttttaat ctgcgatctc
    10981 ctggtgtgcc atgaggtgat gcggcatgat ctccaaataa aaccatcgta tcaaaactca
    11041 tttcattgac gccatattgt ttgatagttt gctcaatatg gttgactact tcacgttcag
    11101 tcacaccttc ttttaaataa gaaacaccta tttcgataca cttatctgct aactcagcag
    11161 ctttacgtat tttgctaatt tcatcttcgg atttaatatt tcttaattgt ttgattgtta
    11221 aatcaacatc tccgaatgaa ttgacattga aaccagagat taattgtttt tggcgtgcta
    11281 ctgttaagtg ctcgctttca attagtaatt tattgattgt ttgaggataa agtgaaaaag
    11341 gattttcagt gtctaaatat ccaacgattt cacctgtgaa aggtgatgct ttgacttctt
    11401 cgacttccat ttttggacaa aatagtactt gtttaccatc tttcttaatc aataatgcaa
    11461 ataatctttc atggggttcg ctacggtatc cagtaaaata atatacattc aacggtgttg
    11521 taatccatgc tgcatcagct tgttgattgt tcagttcgtc tattattttt gatatttttg
    11581 tcatttcata aacactcctc aacataatat tacttccatt ttatgccaat tattattaga
    11641 tttcaaaatc gagctaatac attaactaga ttttcaattt ttaaaatttg agaatttgtt
    11701 ataattaagt ggcaaaaggg tactaaattt atatagcaat tgataggagg atgctcaaat
    11761 gaagttatca tttcatggtc aatcaacgat ttatttagaa gggaacaaca aaaaggttat
    11821 cgtagatccg tttatttcaa ataaccctaa gtgtgatttg aatattgaaa cagtgcaggt
    11881 agactatatc gttttaacgc acggtcattt tgatcatttt ggtgatgttg tggaactagc
    11941 aaagaaaaca ggagcaacag taattggaag tgcagaaatg gctgactatc tttcttcata
    12001 tcacggtgtt gaaaatgttc atggtatgaa tattggtggt aaagcaaact ttgatttcgg
    12061 aagcgttaaa tttgttcaag catttcatag ttcaagtttt acacatgaaa atggtattcc
    12121 agtttattta ggtatgccaa tgggtatcgt gtttgaagtt gaaggaaaaa caatttatca
    12181 tactggtgat acaggtctgt ttagtgacat gtcacttatt gcgaaacgtc atccagttga
    12241 tgtatgtttt gtaccaattg gagataattt tacgatgggt attgatgatg caagttatgc
    12301 tattaatgaa tttattaaac caaaaatttc tgtaccgatt cattatgata cattcccatt
    12361 aatcgaacaa gatccacaac aatttaaaga tgctgttaat gtaggggacg tgcaaatttt
    12421 aaaaccaggt gaatctgttc agttttaaat agaatataga ttttacacta aaaaacacct
    12481 cgctaatcta tgtggaagtt atcgtctgca tatttttagt gggtgttttt atattctact
    12541 gattcagtta cgacgtggac gggggacatc agtggttctt tatcatttct gacctactct
    12601 cgtaatttta gttttataca attagacatt gattataaat ttactacgat ataaaaaatc
    12661 cagaaccaca gtcataatca tttcttgtgt ttactcagtc gtttgagact gcatcatgat
    12721 tgaaagatta tttgtgattc tggataaaaa ttttatttaa cgattaatac agggatagtc
    12781 gcacgttttg ctactttgtg gctgacactt ccaagtacaa atttcttttt gtcttctgct
    12841 ttacggttac ttaaaacaac aatttcgtat ttaccgctat tagcatgttt taatagttct
    12901 tcttttgcat taccacgaac aattatttgg tcataatcaa taccataatc ttctaaagct
    12961 ttgcgtgttt tttccaatcg cttgcttcgt tcttcagtaa gtttgtttaa atgaacacct
    13021 gctttaattg atgcttgagc atcttgttcg ctgattgcgt ttaaaactgt tacgactgta
    13081 ccttcgccag ctaatttaga cacttctttt agtgcttttt cattttttaa ctgagtgtct
    13141 acaccaagta atatattttt atacattagt acatccctcc tcacttacat tctaataaat
    13201 aataatggat ttttccattt aaaattaaag attttgtgat ctttgtgttt gttgtatatt
    13261 ttcaaaaaat aaaataggtg aaacgatcat tatttcattt cacttcaatg ctttttatcc
    13321 aaaataaagt tataatgtag ttaatactgt tcgaaaagaa gaggtaaaga atgacaaaac
    13381 atgaacaaat tttagattat attgaatcgt tatctatagg ctctaaaatt tcggtgcgta
    13441 aaatcgctaa atttttaaac gtatctgaag ggacagcata tcgtgcaatt aaagatgctg
    13501 ataaaatggg catggtcgca acgatagatc gtgttggtac ggttcgaatc gaaaaacgta
    13561 atcgtaatga aattgaacat ttaactttta atgaaattgt caatattatt gatggccaag
    13621 tgttaggtgg caataaaggt atcactaaga tggtatctaa atttgcaatt ggagcaatgg
    13681 aactcaaaga tattttaaaa tatattggtc caaagacatt attgattgtc ggaaatcgcg
    13741 aagatgtgca gattgaagct ttaaaaagag gtacggctat attaattact ggtggattta
    13801 agccatctaa taaagttatt aattttgcga atgaacatga tttacctgtt ctatcatcaa
    13861 gttatgatac attcctagtt gcaaatatta tcaacaaagc actttttaat cagaaaatac
    13921 gaaaagatat cttaatagtt caagatataa tgacgccatt agatgatttg tctgtgctgt
    13981 ttgatacgat gaaaatagca gattataagc gtatggcgaa tcgaactggc catacaagat
    14041 ttccggtagt caatgagtcg tataaattag taggtattgt aacaagtcga gagatgatta
    14101 atacaaagga cgacgacgaa atagataaag tgatgacgcg aaaccctatt tatgtaaatg
    14161 ctatgagtac agtagcaagt tgtgcgcata tgatgatttg ggaaggtatt gaattgattc
    14221 ctgtcgtttc gagtaataaa aagacagtgg gtgtgattaa tagacaagat gttttaaagt
    14281 ctatgcaact tttaggaaga cagcctcaga tgggtgaaac aattaacgat caaatcgcca
    14341 aatatataac aatgaatcaa gatggaatta cagtagaagt ttcaccactt ttaatcaatc
    14401 attacggaac agtaagtaaa gcggcatttg taagtattat agaagaaaca atacaatatg
    14461 aaatgcgtaa atttaaaaaa ggtaatgtca tgatagagaa cttaaatatc gtgtatatta
    14521 aaacagtacc tattgaatca catataacag tgcgctttgg catactagac gtcggaagaa
    14581 acttcgccaa aatagaagtg aacatgcata gtcaaaacga caaagtagcg agcgctctcg
    14641 tgatatgcca aatgtttgac gaagtatagg aacttagcag agaaaatagg cataagatag
    14701 atttcgcaca aacgttggag cccaacttac attgtttgta gaatttcttt tcgaaattct
    14761 ttgtgttggg gccccacccc aacttgcatc gtctgtagaa attgggggtc caatttcact
    14821 gtgttggggc ccctgaacta aatagtatgg aaatatatat atctaaataa ttaaaacggg
    14881 caaatagttt aatatggcag taacaatttt tataatttag atattttgat atagactaat
    14941 gcttaaatta agcaactttt ctaaaaaagg gagtatgaaa atgattagta ctttgaatga
    15001 aattatgaaa tgtatcgaag acaacgatac aattattata cacagacatg ttagaccaga
    15061 tccagatgca tatggttcac aacttggttt aaaatactat attcagcaga aatttccgca
    15121 aaagcaagta tttgcagtgg gtgaagcgga atcatcatta agttttattg gagagttgga
    15181 taacattgat gataaaacat atcaagatgc gcttgtaatt gtatgtgata ctgccaatgc
    15241 tccacgaatt gacgatgaac gatatagtac aggtagtaaa cttattaaaa tagatcatca
    15301 tcctgcagtt gatcagtatg gtgatattaa tttagttaat acgaacgcgt catctacaag
    15361 tgaaatcatt tatgatttaa tctcacattt taatgatgaa gcaattgtta ataaagatat
    15421 cgcgagtgtt ttataccttg gtatcgtcgg tgatactggg cgattccttt ttaacaatac
    15481 ctcagaacat actatggaaa ttgctggaaa gttaattggg catgatattg atcataatgc
    15541 gctattaaat aaaatgatgg agaaggaccc aaaaatgttg ccgtttcaag gttatgtttt
    15601 acaacatttc gaacttatgg atgatggatt ctgccaagtt aaaataactg aagatgtatt
    15661 ggagcaattc ggtattcagc caaatgaagc atctcagttt gttaatacaa ttgctgacat
    15721 caaaggtttg aaaatatggg tgtttgcagt tgatgagggt agtgaaataa gatgtcgctt
    15781 acgctctaaa gggcaattga ttattaatga tattgcgcaa gattttggtg gcggtggcca
    15841 tccgaatgcg tcaggagttt cagtgaacag ctgggatgaa tttgagcaac ttgctacagc
    15901 tttacgcaca aaacttaact aatagaaagg agccattcaa tcatggtggc atatttaaat
    15961 attcatacgg cttatgattt gttaaattca agcttaaaaa tagaagatgc cgtaagactt
    16021 gctgtgtctg aaaatgttga tgcacttgcc ataactgaca ccaatgtatt gtatggtttt
    16081 cctaaatttt atgatgcatg tatagcaaat aacattaaac cgatttttgg tatgacaata
    16141 tatgtgacaa atggattaaa tacagtcgaa acagttgttc tagctaaaaa taatgatgga
    16201 ttaaaagatt tgtatcaact atcatcggaa ataaaaatga attcaatgga aaatgtttca
    16261 tttgaactat tgcaacaatt ttcatcgaat ttgattatca tttttaaaaa tgttgcagac
    16321 gaacatcgtg acattgttca agtttttgat tcgcatgaag atacgtattt agatcatcaa
    16381 agtgttttag ttcagggtat aaagcacgta tggattcaaa atgtttgtta ccaaacacgt
    16441 caagatgccg atacgatttc tgcattagca gctattagag acaatgcaaa attagactta
    16501 attcatgatc aagaagattt tggtgcacat tttttaactg aaaaggaaat taaacaatta
    16561 gatattaacc aagaatattt aacgcaggtt gatgttatag ctcaaaagtg taatgcagaa
    16621 ttaaaatatc atcaatctct acttcctcaa tatcagacac ctaacgatga atcagctaaa
    16681 aaatatttgt ggcgtgtctt agttacacaa ttgaaaaaat tagaacttaa ttatgacgtc
    16741 tatttagagc gattgaaata tgagtataaa gttattacta atatgggttt tgaagattat
    16801 ttcttaatag taagtgattt aatccattat gcgaaaacga atgatgtgat ggtaggtcca
    16861 ggtcgtggtt cttcagctgg ctcactggtc agttatttac taggtattac aacgattgat
    16921 cctattaaat tcaatctatt atttgaacgt tttttaaacc cagaacgtgt aacaatgcct
    16981 gatatagata ttgactttga agatacacgc cgagaaaggg ttattcagta cgtccaagaa
    17041 aaatatggcg agctacatgt atctggaatt gtgactttcg gtcatctgct tgcaagagcg
    17101 gttgctaggg atgttggacg aattatgggg tttgatgaag ttacattaaa tgaaatttca
    17161 agtttaatcc cacataaatt aggaattaca cttgatgaag catatcaaat tgacgatttt
    17221 aaaaaatttg tacatcgaaa ccatcgacat gaacgctggt tcagtatttg taaaaagtta
    17281 gaaggtttac caagacatac atctacacat gcggcaggaa ttattattaa tgaccatcca
    17341 ttatatgaat atgccccttt aacgaaaggg gatacaggat tattaacgca atggacaatg
    17401 actgaagccg aacgaattgg gttattaaaa atagattttc tagggttgag aaacttatcg
    17461 attattcatc aaatcttaat acaagtcaaa aaagatttag gtattaatat tgatatcgaa
    17521 aagattccgt tcgatgatca aaaagtgttt gaattgttgt cgcaaggaga tacgactggc
    17581 atattccaat tagagtcaga cggtgtaaga agtgtattaa aaaaattaaa gccggaacac
    17641 tttgaagata ttgttgctgt aacttctttg tatagaccag gtccaatgga agaaattcca
    17701 acttacatta caagaagaca tgatccaagc aaagttcaat atttacatcc acatttagaa
    17761 cctatattaa aaaatactta cggtgttatt atttatcaag agcaaattat gcaaatagcg
    17821 agcacatttg caaacttcag ttatggtgaa gcggatattt taagaagagc aatgagtaaa
    17881 aaaaatagag ctgttcttga aagtgagcgt caacatttta tagaaggtgc aaagcaaaat
    17941 ggttatcacg aagacattag taagcaaata tttgatttga ttctgaaatt tgctgattat
    18001 ggttttccta gagcacatgc tgtcagctat tctaaaattg catacattat gagcttttta
    18061 aaagtccatt atccaaatta tttttacgca aatattttaa gtaatgttat tggaagtgag
    18121 aagaaaactg ctcaaatgat agaagaagca aaaaaacaag gtatcactat attgccaccg
    18181 aacattaacg aaagtcattg gttttataaa ccttcccaag aaggcattta tttatcaatt
    18241 ggtacaatta aaggtgttgg ttatcaaagt gtgaaagtga ttgttgatga acgttatcag
    18301 aacggcaaat ttaaagattt ctttgatttt gctagacgta taccgaagag agtcaaaacg
    18361 agaaagttac ttgaagcact gattttagtg ggagcgtttg atgcttttgg taaaacacgt
    18421 tcaacgttgt tgcaagctat tgatcaagtg ttggatggcg atttaaacat tgaacaagat
    18481 ggttttttat ttgatatttt aacgccaaaa cagatgtatg aagataaaga agaattgcct
    18541 gatgcactta ttagtcagta cgaaaaagaa tatttaggat tttatgtttc gcaacaccca
    18601 gtagataaaa agtttgttgc caaacaatat ttaacgatat ttaaattgag taacgcgcag
    18661 aattataaac ctatattagt acagtttgat aaagttaaac aaattcgaac taaaaatggt
    18721 caaaatatgg cattcgtcac attaaatgat ggcattgaaa ctttagatgg tgtgattttc
    18781 cctaatcagt ttaaaaagta cgaagagttg ttatcacata atgacttgtt tatagttagc
    18841 gggaaatttg accatagaaa gcaacaacgt caactaatta taaatgagat tcagacatta
    18901 gccacttttg aagaacaaaa attagcattt gccaaacaaa ttataattag aaataaatca
    18961 caaatagata tgtttgaaga gatgattaaa gctacgaaag agaatgctaa tgatgttgtg
    19021 ttatcctttt atgatgaaac gattaaacaa
//
LOCUS       DAHHSC010000030        18983 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.030, whole genome shotgun sequence.
ACCESSION   DAHHSC010000030 DAHHSC010000000
VERSION     DAHHSC010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 18983)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18983)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18983
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.030"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            150..524
                     /locus_tag="NBI57_000830"
     CDS             150..524
                     /locus_tag="NBI57_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4467 domain-containing protein"
                     /protein_id="HCD1821794.1"
                     /translation="MKRILVVFLMLAIILAGCSNKGEKYQKDIDKVYKEQNQMNKIAS
                     KVQNTIKTDIKQEDSNTHVYKDGKVIVIGIQLYKDREKMYYFAYEIKDGKAEINREID
                     PIKYMKDHKADYEDENVEVEKD"
     gene            674..1072
                     /locus_tag="NBI57_000831"
     CDS             674..1072
                     /locus_tag="NBI57_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HCD1821795.1"
                     /translation="MEKSIKIMTIIGIVIQGLATVFSLLLMVLAASGVMTTDVSTTVN
                     GEVDPVDAETAAAIFTVLFLSLFIFGIISIILGAIGMFKASKNKKMSGILLIIGAVIS
                     GNIITFALWLVSGIKLLTNNKPKDEISDLS"
     gene            complement(1288..2112)
                     /locus_tag="NBI57_000832"
     CDS             complement(1288..2112)
                     /locus_tag="NBI57_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HCD1821796.1"
                     /translation="MKEKHIKWDKIFYGDDWVNNVVETIRTKDILQSVYLKRYLLRAM
                     MAGFIIGIITVFVLSVKATHEPDLPPGIVNMASAITFSFALVLILFTNSELLTSNFMY
                     FTVGLYYKVIKPTRVLKIFLLCFAGNILGAAILFSFMRFSNVMTPDMLNQLSAVIEHK
                     TLSTGFVSILMKAIFANFFINISLVIAMQIDDVLAKMFVMMFGVTIFAFMGYEHVVYN
                     SCLFMGGLIYQVDTLHFIPAISNIAAAFIGNYIGGGLIIGLFYAYLNDHHQFYKNN"
     gene            complement(2349..3656)
                     /gene="brnQ"
                     /locus_tag="NBI57_000833"
     CDS             complement(2349..3656)
                     /gene="brnQ"
                     /locus_tag="NBI57_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HCD1821797.1"
                     /translation="MKKQVIISGLMLFSLFFGAGNLIFPPILGHTAGQNMWIGMLGFA
                     LTGILLPFITVIVVAFYDEGVESVGNRIHPWFGFIFAVVIYMSIGAFYGIPRAANVAY
                     EIGTRHILPVHNQWTLIIFAAIFFAIVYWISLNPSKIVDNLGKLLTPLLLLMVALLSI
                     AVIFNPESALSAPKDKYITHPFISGSLEGYFTMDLVAALAFSVVIVNGYKFKGLTDRM
                     KILKYVCFSGLIAAILLGMIYFALAYVGASTAPGNFKDGTDILTYNSLRVFGSFGNIV
                     FGMTVILACLTTCIGLVNACATFTKKHVPKFSYKIFALIFSIIGFLFTTLGLEMILKI
                     AVPLLTLIYPVSIALVLISFANMFSTFRFSWAYRLATVITLIISILQILNSFNLLHGV
                     ILKWFMMLPLADIDLAWLVPFMLFAIIGFIIDVVIRRPKQATT"
     gene            4240..5130
                     /locus_tag="NBI57_000834"
     CDS             4240..5130
                     /locus_tag="NBI57_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase, lipoprotein e(P4) family"
                     /protein_id="HCD1821798.1"
                     /translation="MNKISKYIAIASLSVAVTVSAPQTTNSTAFAKSSAEVQQTQQAS
                     IPASQKANLGNQNIMAVAWYQNSAEAKALYLQGYNSAKTQLDKEIKKNKGKHKLAIAL
                     DLDETVLDNSPYQGYASIHNKPFPEGWHEWVKAAKAKPVYGAKEFLKYADKKGVDIYY
                     ISDRDKEKDLKATQKNLKQQGIPQAKKSHILLKGKDDKSKESRRQMVQKDHKLVMLFG
                     DNLLDFTDPKEATAESREALIEKHKDDFGKKYIIFPNPMYGSWEATIYNNNYKASDKA
                     KDKLRKNAIKQFDPKTGEVK"
     gene            5379..6428
                     /locus_tag="NBI57_000835"
     CDS             5379..6428
                     /locus_tag="NBI57_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCD1821799.1"
                     /translation="MFLAWNEIRRNKLKFGLIIGVLTMISYLLFLLSGLANGLINMNK
                     EGIDKWQADAIVLNKDANQTVQQSVFNKKDIENKYKKQATLKQTGEIVSNGHQKDNVL
                     VFGVEKSSFLVPSLIEGHKATKDNEVLADETLKNKGFKIGDTLSLSQSDEKLHIVGFT
                     ESAKYNASPVIFTNDATIAKINPRLTGDKINAVVVRDTNWKDKKLNQELEAVSINDFI
                     ENLPGYKPQNLTLNFMISFLFVISATVIGIFLYVMTLQKTSLFGILKAQGFTNGYLAN
                     VVISQTLILALFGTAFGLLLTGVTGAFLPDAVPVKFDVLTLLVFAIVLMIVSVLGSLF
                     SILTIRKIDPLKAIG"
     gene            6441..7118
                     /locus_tag="NBI57_000836"
     CDS             6441..7118
                     /locus_tag="NBI57_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCD1821800.1"
                     /translation="MLKFENVTKSFKDGNRNIEAVKDTNFEINKGDIIALVGPSGSGK
                     STFLTMAGALQTPTSGHILINNQDITTMKQKALAKVRMSEIGFILQATNLVPFLTVKQ
                     QFTLLKKKNKNVMSNEDYQQLMSQLGLTSLLNKLPSEISGGQKQRVAIAKALYTNPSI
                     ILADEPTAALDTENAIEVIKILRDQAKKRKKACIIVTHDERLKAYCDRSYHMKDGVLN
                     LENETVE"
     gene            7326..8357
                     /locus_tag="NBI57_000837"
     CDS             7326..8357
                     /locus_tag="NBI57_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000364852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HCD1821801.1"
                     /translation="MDLMIGTLFLFLVLVIFTLFTYKAPNGMRAMGALANAAIATFLV
                     EAFNKYVGGEVFGIKFLEELGDAAGGLGGVAAAGLTALAIGVSPVYALVIAAACGGMD
                     LLPGFFAGYMIGYVMKYTEKYVPDGVDLIGSIVILAPLARLIAVLLTPVVNSTLIRIG
                     DIIQSSTNTNPIIMGIILGGIITVVGTAPLSSMALTALLGLTGVPMAIGAMAAFSSAF
                     MNGTLFHRLKLGDRKSTIAVSIEPLSQADIVSANPIPIYITNFFGGAIAGLIIAMSGL
                     INDATGTATPIAGFLVMFGFNHPMTIVIYGVVMAIVGALAGYLGSIVFKKYPIVTKQD
                     MINRGAVDA"
     gene            8700..9818
                     /locus_tag="NBI57_000838"
     CDS             8700..9818
                     /locus_tag="NBI57_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HCD1821802.1"
                     /translation="MSDSEKEILKRIKDNPFISQRELAEAIGLSRPSVANIISGLIQK
                     EYVMGKAYVLNEDYPIVCIGAANVDRKFYVHKDLVAETSNPVTSTRSIGGVARNIAEN
                     LGRLGETVAFLSASGQDSEWEMIKRLSTPFMNLDHVQQFENASTGSYTALISKEGDMT
                     YGLADMEVFDYITPEFLIKRSHLLKKAKCIIVDLNLGKEALNFLCAYTTKHQIKLVIT
                     TVSSPKMKNMPDSLHAIDWIITNKDETETYLNLKIESTDDLKIAAKRWNDLGVKNVIV
                     TNGVKELIYRSGEEEIIKSVMPSNSVKDVTGAGDSFCAAVVYSWLNGMSTEDILIAGM
                     VNAKKTIETKYTVRQNLDQQQLYHDMEDYKNGKFTKVY"
     gene            9793..10716
                     /locus_tag="NBI57_000839"
     CDS             9793..10716
                     /locus_tag="NBI57_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HCD1821803.1"
                     /translation="MANLQKYIEYSREVQQARENNQPIVALESTIISHGMPYPQNVEM
                     ATTVEQIIRNNGAIPATIAIIDGKIKIGLESEDLEILATSKDVAKVSRRDLAEVVAMK
                     CVGATTVATTMICAAMAGIQFFVTGGIGGVHKGAEHTMDISADLEELSKTNVTVICAG
                     AKSILDLPKTMEYLETKGVPVIGYQTNELPAFFTRESGVKLTSSVETPERLADIHLTK
                     QQLNLEGGIVVANPIPYEHALSKAYIEAIINEAVVEAENQGIKGKDATPFLLGKIVEK
                     TNGKSLAANIKLVENNAALGAKIAVAVNKLL"
     gene            10727..11947
                     /locus_tag="NBI57_000840"
     CDS             10727..11947
                     /locus_tag="NBI57_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HCD1821804.1"
                     /translation="MNILFAITGIAFALFVAFLFSFDRKNIDFKKTLIMIFIQVLIVL
                     FMMNTTIGLTILTALGSFFEGLINVSKAGINFVFGDIQNKNGFTFFLNVLLPLVFISV
                     LIGIFNYIKVLPFIIKYVGIAINKITRMGRLESYFAISTAMFGQPEVYLTIKDIIPRL
                     SRAKLYTIATSGMSAVSMAMLGSYMQMIEPKFVVTAVMLNIFSALIIASVINPYKSDD
                     SDVEIDNLTKSTETKSVNGKTGKPKKVAFFQMIGDSAMDGFKIAVVVAVMLLAFISLM
                     EAINIMFGSVGLNFKQLIGYVFAPIAFLMGIPWSEAVPAGSLMATKLITNEFVAMLDF
                     KNVLGDVSARTQGIISVYLVSFANFGTVGIIVGSIKGISDKQGEKVSSFAMRLLLGST
                     LASIISGSIIGLVL"
     gene            complement(12052..13584)
                     /locus_tag="NBI57_000841"
     CDS             complement(12052..13584)
                     /locus_tag="NBI57_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HCD1821805.1"
                     /translation="MKEVGFGTLNWVAVIIYLLAMLFIGVYFTKRASQSTNSFFTASG
                     RLPSWVVGFSIYATTLSAITFMSTPEKAFLTDWSYIAGNIAIVAIIPLLIYFYVPFFK
                     KLKVTSAYEYLEARFGPSIRVIGSLLFVVYHLGRVAIVIYLPTLAITSVSDMNPYIVA
                     SLVGLLCILYTFLGGFEGVVWSDFIQGVILLGGALVIIILGVMNIKGGFGTVFADAIE
                     HKKLISADNWKLNTAAAAIPIIFLGNIFNNLYQYTASQDVVQRYQASDSLKETNKSLW
                     TNGILALISAPLFYGMGTMLYSFYTHEAVLPKGFNTSSVVPYFILTEMPPFVAGLLIA
                     AIFAAAQSTISSSLNSISACISIDIKQRFFGKDSERHEVNFARFIIIIAGIFGFGMSL
                     YLIASNSNDLWDLFLFVTGLFGVPLAGVFAVGIFTKRTNTFGVICGLILGIIFAYVYN
                     GVGKGNSPFYVSTISFTVAFVFAYILSFIVPSKHKKDITGLTIFEKDKPSTYISKTAT
                     NK"
     gene            complement(13624..14505)
                     /locus_tag="NBI57_000842"
     CDS             complement(13624..14505)
                     /locus_tag="NBI57_000842"
                     /EC_number="4.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HCD1821806.1"
                     /translation="MNKDLKGLYAALLVPFDENGQVNEQGLKQIAQNAIETEELDGLY
                     VNGSSGENFLLNTEQKKQVFKVAKEAVGDKVKLIAQVGSLDLNEAIELGKYATELGYD
                     ALSAVTPFYYPFTFEEIRDYYFDIIEATQNNMIIYAIPDLTGVNISIEQFSELFNHEK
                     IVGVKYTAPNFFLLERIRKAFPDKLILSGFDEMLVQATISGVDGAIGSTYNVNGRRAR
                     KIFDLARQGQIQEAYQLQHDSNDIIETVLSMGIYPTLKEILRHRDIDAGLPKRPFKPF
                     NEAHRQTLDQLIAKYDL"
     gene            14663..15523
                     /locus_tag="NBI57_000843"
     CDS             14663..15523
                     /locus_tag="NBI57_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HCD1821807.1"
                     /translation="MYYIAIDIGGTQIKSAVIDKQLNMFDYQQISTPDNKSELITDKV
                     YEIVTGYMKQYQLIQPVIGISSAGVVDEQKGEIGYAGPTIPNYKGTNFKRLLKSLSPY
                     VKVKNDVNAALLGELKLHQYQAERIFCMTLGTGIGGAYKNNQGHIDNGELHKANEVGY
                     LLYRPTENTTFEQRAATSALKKRMIDGGFTRSTHVPVLFEAAEEGDDIAKQILNEWAE
                     DVAEGIAQIQVMYDPGLILIGGGISEQGDNLIKYIEPKVAHYLPKDYVYAPIQTTKSK
                     NDAALYGCLQ"
     gene            complement(15800..16600)
                     /locus_tag="NBI57_000844"
     CDS             complement(15800..16600)
                     /locus_tag="NBI57_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HCD1821808.1"
                     /translation="MKFENRVQRYQHLFTKTDKRIVNYIRQNGYSDAFSTINSLAHAI
                     GTSPATMTRFSHKLDYENFQDLKFNIQQEMTETVIENSPIIQRIHKYHQQIIQQTGEF
                     IDNDIIQTFIDKLQSSRHILFAGLGSSGLSATEFYYRMIRMGLKGNVTTDSHLMKISA
                     SLLSHSDMFIAMSNSGNTSELISAAEVAKSHGAYVVAITNFEGSKLTDCANLVLLTTD
                     QSRNTDHQFINTQIATLFLIDIVSYHLLENTNLSQTYQHTKSIILDNK"
     gene            16740..17408
                     /locus_tag="NBI57_000845"
     CDS             16740..17408
                     /locus_tag="NBI57_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HCD1821809.1"
                     /translation="MLPHGLIVSCQALADEPLHSSFIMSKMALAAYEGGAVGIRANTK
                     EDILAIKETVDLPVIGIVKRDYDHSDVFITATSKEVDELIESQCEVIALDATLQQRPK
                     ETLDELVSYIRTHAPNVEIMADIATVEEAKNAARLGFDYIGTTLHGYTSYTQGQLLYQ
                     NDFQFLKDVLQSVDAKVIAEGNVITPDMYKRVMDLGVHCSVVGGAITRPKEITKRFVQ
                     VMED"
     gene            complement(17539..18852)
                     /locus_tag="NBI57_000846"
     CDS             complement(17539..18852)
                     /locus_tag="NBI57_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiH family protein"
                     /protein_id="HCD1821810.1"
                     /translation="MNQYHSNAQQPSAWRFFVYSLVGILCFFIPFTINGNNTIFVDHV
                     HLAIRSIIGPLMPYVALIMILIGTALPIVRRTFMTSITNLVITLFKVAGAMIGIMYVF
                     KIGPSILFKANYGPFLFEKLMMPLSILIPVGAIALSLLVGYGLLEFVGVYMEPIMRPI
                     FKTPGKSAVDAVASFVGSYSLGLLITNRVYKQGMYNKREATIIATGFSTVSATFMIIV
                     AKTLGLMPHWNLYFWITLVITFVVTAITAWLPPITNESTEYYNGQEGEQEVAIEGSRL
                     KTAYAEAMKQNALTPSLVKNVWDNFKDGLEMTVGILPSILSIGFLGLIVANYTPFIDW
                     LGYIFYPFIYIFPIADQAVLAKASAISIVEMFLPSLLVTKAAMSTKFVVGVVSVSAII
                     FFSALVPCILATEIKIPVWKLIIIWFLRVALSLLITIPVALLIFG"
BASE COUNT         6699 a         2890 c         3520 g         5874 t
ORIGIN      
        1 atcataatta atcaaaatct tgcgtcaaac atttaaacga attttaagtt gataacaatt
       61 ggttaaacat tgttcaaaca tcacaatgat aaagcatatt atcagtattg tagtgtgtgg
      121 aaaatgacag ccatctaagg agaaaaatga tgaaaagaat attggtagta tttttaatgt
      181 tagcaattat attggcaggt tgttctaata aaggtgaaaa gtatcaaaaa gatattgata
      241 aagtgtacaa agaacagaat caaatgaata aaattgcctc gaaagtacaa aacactatta
      301 aaacagacat taaacaagaa gacagtaata cacatgtcta taaagatggt aaagtcattg
      361 ttattggtat tcaattatat aaagatcgtg aaaaaatgta ttatttcgca tatgaaataa
      421 aagatggtaa ggcagagatt aatagagaaa tagacccaat taagtatatg aaagaccata
      481 aagcagatta tgaagatgaa aatgtagaag tggaaaaaga ttaacgtagg ctttctttaa
      541 aacttttaac cagtttcaaa aagtaaatga gctttagcga caataatttt taaaataaac
      601 acgatataaa tttaaacatt tatatataag aacacaaacg taagtttgta aataataata
      661 aaggagtaag tcaatggaaa aatcgatcaa aataatgaca ataataggaa ttgttattca
      721 gggtttagca acggtattta gtttactatt gatggtttta gcagcatcag gtgtaatgac
      781 tacagatgtg tcaacaacag ttaatggtga ggttgaccca gttgatgcag aaacagcagc
      841 agcaattttc actgtattat tcttatccct attcatattt ggaatcattt caattatttt
      901 aggtgcaatc ggtatgttta aagcatctaa aaacaaaaaa atgagtggta tattgttgat
      961 tattggagct gtaataagtg gtaacataat tacatttgct ttatggttag tcagtggtat
     1021 taaactactt actaataaca agcctaaaga tgaaataagc gacttatcat aaacatcgta
     1081 tattgaaatt tcaagatttc ttaagtaatt aaataaagtg ctctcttaga agtagatttt
     1141 cagtcaataa ctgcttttaa gagagcattt tgtatttgat aatgctcata gcatcggaca
     1201 aaaaccattg acgaacttaa aaaaaatacg ttagacaatg cacttctaaa tatttaaggc
     1261 atcgactaac gcattgttca aatatagtta attattttta taaaattgat gatgatcatt
     1321 caagtaagca tagaataaac ctataatgag tccgcctcca atatagttac cgataaaagc
     1381 cgcagcgata ttcgaaatag ctggtatgaa gtgcaatgta tcaacttgat aaattaaacc
     1441 acccataaat aagcaactgt tgtaaacgac gtgttcataa cccataaagg cgaatatggt
     1501 aacaccgaac atcatgacaa acatttttgc gagtacatcg tcaatttgca tggcaataac
     1561 taatgaaata ttgataaaga aattggcgaa gatcgctttc attaatatac ttacaaaacc
     1621 agtagacaac gttttgtgct caataactgc tgataactga tttaacatat ctggcgtcat
     1681 tacatttgaa aaacgcatga aactaaataa aatagcagca cctaaaatat ttcctgcaaa
     1741 gcataataaa aatattttca atactctagt cggtttaatt actttataat acaggcctac
     1801 agtaaagtac atgaagttac tggttagtag ttcggagttt gtaaataaaa tgagtactaa
     1861 cgcaaagctg aatgtaatgg cgctagccat attcactata cctggtggta aatctggttc
     1921 gtgtgttgct ttaactgata gcacgaagac cgtaataata ccgataataa atcctgccat
     1981 catcgcgcgt aataaataac gttttaaata aacgctttgt aatatatctt ttgttcttat
     2041 tgtttcgact acgttattta cccagtcatc cccataaaaa atcttatccc atttaatatg
     2101 tttctccttc acaatgacat cctcgctttt catttgttaa tcagagtata ctatgaatcg
     2161 tcttatttgt gaataatttc acaaaattaa ttttaaaaac taatattttt gaataactga
     2221 taggattttt tggtaaatca attattttaa aataatcaat agacagacag tatcgctgaa
     2281 aatccatgaa tttcatataa aaaagcctag taacgagggg gcaatcatta ctaggcagtg
     2341 agcatttatt aagttgtcgc ttgtttcgga cgacgtataa ctacatcgat gatgaaaccg
     2401 ataatagcaa agagcatgaa tggtacaagc caagctaaat cgatatctgc taaaggtaac
     2461 atcataaacc atttcaaaat aacaccgtgt aataagttga aactatttag tatttgtaaa
     2521 attgaaataa tcaatgtaat aacagttgcg agtcgatagg cccaactgaa tctgaatgtg
     2581 ctaaacatgt tagcaaatga tatcagtaca agtgcaatag acacgggata tattaatgtc
     2641 aacaatggta cagcaatttt taaaatcatt tctaaaccaa gtgttgtaaa taagaaccct
     2701 atgatagaga aaataagtgc gaatatttta taagaaaact taggtacgtg tttcttagta
     2761 aatgtggcgc aagcattgac gagtcctata catgttgtta aacatgcaag tatcactgtc
     2821 ataccaaata ctatgttgcc aaatgaacca aatactcgta atgagttgta cgtcaatata
     2881 tctgtaccat ctttaaagtt tcctggagct gttgatgccc caacgtatgc aagtgcaaag
     2941 taaatcattc caagtaatat ggctgcaata agacctgaaa agcagacata ttttaaaatt
     3001 ttcatgcgat ctgtgaggcc tttaaactta taaccattga caatgactac ggaaaaagct
     3061 aacgcagcaa caagatccat tgtaaaatag ccttccaaac ttcctgaaat gaaaggatgt
     3121 gttatatatt tatccttagg tgcacttagt gcagattcag ggttgaaaat gacagcaata
     3181 cttaatagag cgaccattaa tagtaataat ggtgttaata atttacctaa attatcaacg
     3241 attttcgatg gatttaaact aatccagtaa acgatggcaa aaaagattgc tgcgaatata
     3301 attaaagtcc attggttatg cacaggtaaa atgtgtcttg taccaatttc gtacgcgaca
     3361 tttgcagcac gtggaatacc gtaaaatgct ccgatagaca tgtaaatcac gacagcaaaa
     3421 ataaacccga accatggatg tatacgattg cctacacttt caacaccttc atcataaaat
     3481 gcaacaacaa taacagtaat aaaggggagt aatatgcctg taagggcaaa gcctagcata
     3541 ccaatccaca tattttgacc cgctgtatgg ccaagaatgg gcgggaatat taaatttccg
     3601 gctccaaaaa atagtgaaaa taacatgagg cccgaaataa taacttgttt tttcaatgta
     3661 atttccttcc taaaactaga tattcataat aatttaaaaa atctgaaaaa taaaaacgtt
     3721 cttactttat ctttaaatga caatactatg attctattat tttataaaaa agattgcaat
     3781 aataacgtct tgctattatt ggataatgac aattaaagtg tcgtttttat gaattaaaat
     3841 aggtgaaagt aagttggaaa tacgttggag cgttgtgatt tttatgagaa attgtatgag
     3901 tgaggactgc tatttataaa aggtaaacta tggatgagtt tatggttgtt ccaataggta
     3961 aataagagat agcacactgt acacataatg atacgtggat gagtgaatat gtctgatagg
     4021 aaggcctagt cactcaaatg aatttaagtt tattacgtga taaatcacaa atctctctca
     4081 tgtgataggt ctcccattaa atcatgctta tatgaaatgt tcacatattt gttagctttt
     4141 caagaaataa tattaaatat cttttcatag tatagcaata ttaaataaat gcatttataa
     4201 ttttaactgt attttaaata ttaatcatga ggtgataaga tgaataaaat ttcaaagtat
     4261 attgcaatag catcattatc ggtagcggtt acagtttcag caccacaaac gacaaattct
     4321 acagcgtttg ccaaaagttc tgctgaagtt caacaaacgc aacaagcttc tataccagca
     4381 tcacaaaagg cgaatcttgg taatcaaaat attatggcag tggcttggta tcaaaattca
     4441 gctgaagcaa aagcattata tttacaaggt tataacagtg caaagacaca gttagataaa
     4501 gagattaaaa agaataaagg taaacataag ttagctattg ctttggattt agatgaaaca
     4561 gttttagata attctccata tcaaggctat gcatcaatac ataataaacc tttcccagaa
     4621 ggttggcatg aatgggtaaa agctgctaaa gctaaacctg tctatggcgc aaaagaattc
     4681 ttgaaatatg ctgacaaaaa aggtgtcgat atctactata tttctgatag agataaagaa
     4741 aaagatttaa aggcaacaca aaagaactta aaacaacaag gtatccctca agctaagaag
     4801 agtcatattt tactaaaagg taaagatgat aagagtaaag aatcacgcag acaaatggtt
     4861 caaaaggatc ataaacttgt catgctattt ggagataatt tattagactt tacagatcca
     4921 aaagaagcta cagctgaatc tcgtgaagca ttaattgaaa aacataaaga cgatttcggt
     4981 aagaaatata tcattttccc taacccaatg tatggtagtt gggaagctac gatttacaac
     5041 aataactata aagcaagtga caaagcaaaa gataaattac gtaaaaatgc tattaagcaa
     5101 ttcgatccta aaacaggcga agttaaataa tatatgaatt ggacgtatac atgtacttaa
     5161 aggtatatgt aggcgttttt aaattgatcc ttaattttaa atgtaccgta caaaaataat
     5221 agacaattaa ttgcattttc cataaatttg cttttaatat aaaaagttcg ggtataatga
     5281 aggataagtg tatgtataac attacagagc gcaacacgac gtacgtgcat ctaaggatat
     5341 aaacaatgtt taaatcatag tagtagggag agaaatgcat gtttttagct tggaatgaaa
     5401 tacggcgcaa caaattgaag tttggactaa ttattggtgt gttaacgatg attagttact
     5461 tgctattttt attatctgga ttggcgaatg gtcttatcaa tatgaataaa gaaggcattg
     5521 ataagtggca agcagatgcc attgttctaa ataaagatgc caatcaaact gtgcaacaat
     5581 ctgtttttaa caagaaagat attgaaaata aatacaagaa gcaagctact ttgaagcaaa
     5641 caggggaaat tgtgtctaat ggccatcaaa aagacaatgt tttagtgttc ggtgttgaaa
     5701 agtcatcatt tttagttccg agtttaatag aagggcataa agcgactaaa gataatgaag
     5761 tgttagctga tgaaacgctt aaaaataaag gatttaaaat tggtgacaca ttatcactat
     5821 ctcaatcaga tgaaaaattg catatcgtag gttttacaga aagtgcaaaa tataatgcgt
     5881 caccagtcat tttcacgaat gacgctacca ttgccaagat caatcctaga ttgactggag
     5941 ataaaattaa tgcagttgtt gtacgtgata caaattggaa agacaaaaaa ttaaaccaag
     6001 agcttgaagc ggtaagtatt aatgacttta ttgaaaattt accaggttat aaaccacaga
     6061 acttaacatt aaactttatg atttcattct tatttgtcat ttcagctaca gttataggca
     6121 ttttcttata tgtcatgaca ttacaaaaaa caagtttatt tggcatatta aaggctcaag
     6181 gatttacgaa tggctattta gcgaacgtag ttatttcgca gacgctcata ttagcattat
     6241 ttggtacggc atttggctta ctgttaacag gcgttacagg tgcattttta cctgatgcag
     6301 tacctgtcaa attcgatgta ctaacattgc tcgtatttgc aattgtgtta atgattgtct
     6361 ctgtattagg aagtttattc tccattttaa caattagaaa aatagatccg ttaaaggcga
     6421 ttgggtagga ggtgtagcaa atgttgaaat ttgaaaatgt aacaaagtca tttaaagatg
     6481 ggaatcgtaa cattgaagcg gttaaagata caaattttga gataaataaa ggtgatatta
     6541 tagcattggt tggaccttct ggctctggta aaagtacatt tctaactatg gcaggtgctt
     6601 tacaaacgcc gacatctggg cacattttaa tcaataacca agatattacg acaatgaagc
     6661 aaaaagcatt ggcaaaagtt agaatgtctg aaataggttt tattttacaa gctacaaacc
     6721 ttgtaccatt tttaacggta aagcaacaat ttacattatt gaaaaagaaa aataagaatg
     6781 ttatgtctaa tgaagactat cagcaactta tgtcacaatt aggtctaact tcattgctta
     6841 ataagttacc ttcagaaatt tcaggtggtc agaaacaacg tgtggcgata gccaaagcgt
     6901 tatatacgaa tccgtcgatt attttagcgg atgaacctac cgcggcgtta gatactgaaa
     6961 atgcgattga agtcattaaa attctacgtg atcaagccaa aaaaagaaag aaagcatgta
     7021 ttattgttac acatgatgaa cgacttaaag catattgtga tcgttcatat catatgaaag
     7081 atggcgtcct taatcttgaa aatgaaacag tagaatagtt ttattaagcc ggtacatcat
     7141 gtgccggtat ttttatgttt atgtattatt tgaataaact ttcacattca attaataata
     7201 attattatcg aaaattagaa atattccgtg aaatataata ttttttgtag taaaatggcc
     7261 tctaagtatt caatatttaa atatggggat tgaatataaa attatcgtaa tgggggtcaa
     7321 tggttatgga tttaatgata ggtactttat ttttattttt ggtcttagtg atttttacat
     7381 tatttacata taaagcacct aatggtatgc gtgccatggg agcattagct aatgcagcaa
     7441 tcgcaacatt tttagtggaa gcatttaata aatatgttgg tggcgaagta ttcggtatta
     7501 aatttttaga agagctagga gacgctgcgg gaggtctagg tggtgtcgct gccgctggat
     7561 taacagcatt agctatcggt gtatcaccag tatatgcatt agttatagca gccgcgtgcg
     7621 gtggtatgga tttattacca ggtttctttg cgggttatat gattggatat gtgatgaaat
     7681 atacagagaa atatgtgccg gatggtgtcg acttaattgg atcgattgtc atcttagcgc
     7741 cattagctcg tcttattgca gtattattaa cgccagtagt gaatagtaca ttgattcgaa
     7801 ttggtgatat tatccaaagt agtacgaata cgaatccaat tatcatgggt atcattttag
     7861 gtggtattat cacggttgtc ggcacagcgc cattgagttc aatggcattg acagcattat
     7921 taggtttaac gggtgtacct atggctattg gtgccatggc agcatttagt tcggcattta
     7981 tgaatgggac gctattccat cgcttaaaat taggtgatcg taagtctacg attgcagtaa
     8041 gtattgaacc tttatcacaa gcagatattg tatcagccaa tccaattcca atctatatta
     8101 caaatttctt tggtggtgcg attgctggtt taattattgc tatgtcaggt ttaattaacg
     8161 atgcgacagg tacagctaca ccgattgcag gatttttagt aatgtttgga tttaatcatc
     8221 caatgactat tgtaatttat ggtgtagtaa tggcgattgt aggtgcgctt gcaggttatc
     8281 ttggttcaat cgtatttaaa aaatatccaa ttgttactaa gcaagacatg attaatcgag
     8341 gtgcagttga cgcatagcat catcatattg aatagtaaaa acaaataaaa catagtaacg
     8401 tgattcagtc gatgaaaaaa tcgataatga gtcacgtttt tttatagaaa aatataagac
     8461 ataaaaatgt cataatttat tgtcgacaaa tatcatactg tataaacatt tatcattttc
     8521 tcaagtacct tttacacgat ggaatgaact tactttttac gaaattatgc gtattttata
     8581 aacaaatatc attgatataa cggcaaatgt aagcgtttac aacagaaata acagcatgct
     8641 acgatatttt tgtaaattca ctgattcaag tattttaagt caatatgagg agggatgtta
     8701 tgagcgattc tgagaaagaa attttaaaaa gaattaaaga taatccgttt atttcacaac
     8761 gtgaacttgc tgaggcaatt ggattatcta gacccagcgt agcaaacatt atttcaggat
     8821 taatacaaaa ggaatatgtt atgggaaagg catatgtttt aaatgaagat tatcctattg
     8881 tttgtattgg cgcagcgaat gtagatcgta agttttatgt gcataaagat ttagttgcag
     8941 aaacatcaaa tcctgtaacg tcaacacgct ctattggtgg cgtagcaaga aatattgctg
     9001 agaacttagg taggcttggc gaaacggtcg cttttttatc tgctagtgga caagatagtg
     9061 aatgggaaat gattaaacga ttgtccacac catttatgaa tttggatcat gttcaacaat
     9121 ttgaaaatgc gagtacaggt tcatatacag ctttaattag taaagaaggc gacatgacat
     9181 atggcttagc agatatggaa gtgtttgact acattacgcc tgaattttta attaagcgtt
     9241 cacacttatt gaaaaaggct aagtgcatta ttgtcgattt gaatttaggc aaagaggcat
     9301 taaacttctt atgtgcctat accacgaaac atcaaatcaa attagttatc accacggttt
     9361 cttccccaaa aatgaaaaat atgcctgatt cattacatgc tattgattgg attatcacga
     9421 ataaagatga aacagaaaca tacttaaatt taaaaataga atctactgat gatttaaaaa
     9481 tagctgctaa acgctggaat gatttaggtg ttaaaaatgt tattgtgaca aatggcgtga
     9541 aagaactcat ttatcgaagt ggtgaggaag aaatcatcaa gtcagttatg ccatcaaata
     9601 gtgtgaaaga tgttacaggt gcaggcgatt cattctgtgc tgcagtagta tatagctggt
     9661 taaatgggat gtctactgaa gatatattaa ttgctggtat ggttaacgca aagaaaacga
     9721 tagaaacgaa atatacagtt aggcaaaacc tagatcaaca gcaactttat cacgatatgg
     9781 aggattataa aaatggcaaa tttacaaaag tatattgagt attctcgaga agttcagcaa
     9841 gcacgggaga acaatcaacc gattgtagca ttagaatcaa caattatttc gcatggtatg
     9901 ccgtacccac aaaatgttga aatggcaaca acagtagagc aaattatcag gaataatggt
     9961 gccattccag caaccatagc cattatagat ggcaaaatta aaattggttt agaaagcgaa
    10021 gatttagaaa tactggcaac tagtaaagac gttgctaaag tatctagaag ggatttagca
    10081 gaagttgttg cgatgaagtg tgttggtgct actactgtag cgacgacgat gatatgtgct
    10141 gcaatggctg gtattcaatt ttttgttaca ggaggtattg ggggcgtcca taaaggtgca
    10201 gaacatacga tggacatttc agcagactta gaagaactgt ctaaaacaaa tgtcactgtt
    10261 atctgtgcag gtgccaaatc aattttagac ttacctaaga cgatggagta tttagaaaca
    10321 aaaggcgttc cagttattgg atatcaaacg aatgaattgc cagcattctt cactcgcgaa
    10381 agcggtgtta agttaacaag ttcggttgaa acgccagaac gacttgctga cattcattta
    10441 acaaaacagc agttaaatct tgaaggtggc attgttgttg ctaatccaat tccatatgag
    10501 catgccttat caaaagcata tattgaggca atcataaatg aagctgttgt tgaagcggaa
    10561 aatcaaggta ttaaaggtaa ggacgccaca ccgttcttgt tagggaaaat tgtagaaaaa
    10621 acgaatggta aaagtttagc agcaaatata aaacttgttg aaaacaatgc ggcgttgggt
    10681 gctaaaattg ctgtcgctgt taataaatta ttgtaggtga tgatacatga atattttatt
    10741 cgctatcaca gggatagcat ttgcactatt tgttgcgttt ttattcagtt ttgatcgtaa
    10801 aaacatagac ttcaaaaaga cgttaataat gatatttatt caagtgttga ttgtgttatt
    10861 tatgatgaac acaacgattg gtttaacaat cttaactgca ttaggctcat tttttgaagg
    10921 gttaataaat gttagtaaag caggtataaa ctttgttttt ggagatatac aaaataaaaa
    10981 tggctttacg ttctttttaa atgtgttact gccattagtg ttcatttctg tattaatagg
    11041 catttttaat tatattaagg tattaccatt tattatcaaa tatgtaggta tcgctattaa
    11101 taaaataacg agaatggggc gcttagaaag ttactttgct atttcaacag caatgtttgg
    11161 acaaccagaa gtatatttaa caataaaaga tattattcca agattatcta gagcgaaatt
    11221 atatacaatt gcgacgtctg gtatgagtgc tgttagtatg gcaatgttgg gttcatatat
    11281 gcagatgatt gagcccaagt tcgtagttac agctgtaatg ttaaatattt ttagtgcgct
    11341 tatcatcgct agtgtaatca atccctataa atctgatgat agtgatgttg agattgataa
    11401 tttaactaaa tcaacggaaa cgaaatcagt gaatggaaaa acaggaaaac ctaagaaagt
    11461 tgccttcttc caaatgatag gtgatagtgc gatggatgga tttaaaatcg ctgttgtagt
    11521 agccgtaatg ttgttagcat ttatttcatt aatggaagca attaatatca tgtttggtag
    11581 tgttggtttg aactttaaac agcttattgg ctatgtgttt gcaccaatcg cattcttaat
    11641 ggggattcca tggagcgaag ctgttccagc tggctcttta atggcgacta aattaattac
    11701 aaatgagttt gtagcaatgc ttgattttaa aaatgtcctg ggtgatgtat ctgctcgaac
    11761 acaaggtatc atttcagttt acttagtaag cttcgctaat tttggtacgg ttggtatcat
    11821 cgtaggttca attaaaggca ttagtgataa acaaggagaa aaagtttcat cctttgcaat
    11881 gaggctgtta cttggttcaa ctctagcttc aatcatttca ggatcaatca ttggcttagt
    11941 attgtaaatt aatcgaagta tctaaattaa attcatggca aagctaaacc ccatcaccaa
    12001 gttggcgcaa cagcgcatca taacttggtg atgggatttt atcataacaa tctacttatt
    12061 cgtagccgtt tttgaaatgt atgttgatgg tttatctttt tcaaaaattg ttaatcccgt
    12121 tatatctttt ttatgttttg aagggacaat gaagctaagt atataagcaa agacaaaagc
    12181 aactgtaaat gaaatggtag atacatagaa aggtgagtta cctttgccaa caccattata
    12241 gacataagca aagatgatac ccaatattaa tccacaaata acaccgaatg tattcgtacg
    12301 tttagtgaaa ataccaactg caaatacacc agctaatgga acaccgaata atccagttac
    12361 aaacaagaat aaatcccata agtcatttga attagaagca attaagtata gtgacattcc
    12421 aaaaccgaaa atacctgcaa tgatgataat gaaacgtgca aagttaactt cgtgtcgctc
    12481 gctatctttt ccgaagaagc gttgcttaat gtcgattgaa atacaagcag atatagaatt
    12541 taaactagat gaaatggtag actgtgcagc ggcgaaaatg gctgcaataa gtaatcctgc
    12601 tacaaatggt ggcatctcag tcaaaatgaa atatggcact acagatgatg tattgaagcc
    12661 ttttggtaaa acagcttcat gtgtataaaa tgaatacagc attgtaccca taccataaaa
    12721 taagggtgct gaaattaaag ctaggatacc atttgtccat aacgatttat ttgtttcttt
    12781 taaactatca gaagcttgat aacgctgcac gacgtcttga ctcgctgtgt attgatacaa
    12841 gttgttgaaa atatttccta ggaaaataat tggaatggca gctgccgcag tatttagttt
    12901 ccaattgtct gcactaatta attttttgtg ctcaatcgca tctgcaaaga cagtgccaaa
    12961 accgccttta atgttcataa cacctagaat aataataact aaagcgccgc ctaataaaat
    13021 gacgccttga atgaaatcac tccaaaccac accttcgaaa ccacctaaaa atgtatataa
    13081 aatacatagt aaacctacga gtgatgcaac gatataaggg ttcatgtctg atacagatgt
    13141 gattgctaat gttggtaagt agataacaat tgcaacacgc cctaaatggt aaacgacaaa
    13201 taataatgag ccaatgacac gtatgctagg gccaaatcta gcttctaaat attcatatgc
    13261 agatgttacc tttaactttt taaagaaagg gacatagaaa taaataagta atggaataat
    13321 tgcgacgata gcaatgttac cagcgatata tgaccaatct gttaaaaatg ctttctctgg
    13381 tgtagacata aatgtaatcg cacttaacgt agtagcataa attgaaaagc caactaccca
    13441 agatggcaag cgaccacttg cggtaaagaa actattggta ctttggctcg cgcgcttggt
    13501 aaaataaacg ccaatgaaca acatagctag tagataaatg ataacggcaa cccagtttag
    13561 tgtgccaaat ccaacttctt tcatgggcaa catccccttt acaatgtatt gattctttga
    13621 tgtctataaa tcgtattttg caatgagttg atctaatgtt tgtcgatgtg cttcgttaaa
    13681 aggtttgaaa ggtcttttcg gtaatcctgc atcaatgtca cgatgacgta atatttcttt
    13741 caatgttgga taaatcccca ttgataacac tgtttcgata atgtcgtttg aatcatgttg
    13801 tagttggtaa gcttcttgaa tttgaccttg tcgtgctaag tcgaagattt ttctagcgcg
    13861 gcgaccatta acgttatatg tagaaccaat tgcaccatct acgccagaaa tcgtagcttg
    13921 aactaacatt tcatcaaagc cagataagat taatttgtct gggaatgctt ttctaatacg
    13981 ttcgagtagg aagaagttcg gcgctgtata tttaacacca acaatttttt cgtgattaaa
    14041 tagctcgctg aattgttcaa tagaaatatt cacacctgtt aaatctggta ttgcataaat
    14101 aatcatattg ttctgtgttg cttcgataat atcgaaatag taatctctaa tttcttcaaa
    14161 agtaaatgga tagtagaatg gtgttacggc agaaagtgca tcataaccga gttctgtggc
    14221 atattttccg agttcaatgg cttcatttaa atctaacgaa cctacttgag caatcaattt
    14281 cactttatcc ccaactgcct ctttggcaac cttgaaaact tgcttcttct gctctgtatt
    14341 taataaaaag ttttcgcctg agctaccatt tacataaaga ccgtctaatt cttcagtttc
    14401 aatggcattt tgagcaattt gtttaagtcc ttgttcattt acttgaccat tttcatcaaa
    14461 aggaacgagt aacgctgcat ataaaccttt taaatctttg ttcattatga agtccctcca
    14521 aaaatcattt gataatatag tttacagcta taattgtaaa cgctatcata aaatgtaaca
    14581 atatcttttt gaaaattgta gtcatattta tgtataatta atgaaaatgt ttttcaaaat
    14641 caatagaaat ggagtgagta aggtgtatta catcgcaatc gatattggag gcactcaaat
    14701 taaatcggca gttattgata agcaattgaa tatgtttgac tatcaacaaa tatcaacgcc
    14761 ggacaacaaa agtgagctta ttactgacaa agtatatgag attgtaacag gatatatgaa
    14821 gcaatatcag ttgatccaac ctgtcatagg tatttcatca gcaggcgttg ttgatgaaca
    14881 aaaaggcgaa attggatacg cagggccaac cattccgaat tataaaggta ctaattttaa
    14941 gcgattatta aaatcactgt ctccttatgt caaagtaaaa aatgatgtaa acgctgcatt
    15001 actaggcgaa ttgaaattac atcaatatca agcagaacgg atcttttgta tgacgcttgg
    15061 tacaggcatt gggggtgcgt acaagaataa tcaaggtcat attgataatg gtgagcttca
    15121 taaggcaaat gaagttgggt atttattgta tcgtccaact gaaaatacaa cgtttgagca
    15181 acgtgctgca acgagtgcat tgaaaaagcg catgattgac ggaggattta cgagaagcac
    15241 acatgtgcca gtattgtttg aagcagctga agaaggtgat gatattgcaa aacaaatatt
    15301 gaatgagtgg gcagaagatg tagcagaagg gattgcccaa atacaagtca tgtatgatcc
    15361 agggcttata ttaattggtg gcggtatatc tgaacaagga gacaatctca ttaaatatat
    15421 cgagccgaaa gttgcacact atttaccaaa agactatgtt tatgcaccaa tacaaacgac
    15481 taagagtaaa aatgatgcag cattatatgg ctgtttgcaa tgatagttga aagaaggagt
    15541 cattctaaaa tagaatttga aaccgttacg agagatgaga gctgttgtta gttccacaca
    15601 tcacactcta tctaggacca atctaaacta tatcaaccaa cagtgtgcca cgggcatatt
    15661 aaattgaaga cggttgaacg tatagatctc aaaaatgtaa aaaagtattt ggtattgaaa
    15721 ttaaaaaagc acctagcaac tcgttaggac aatcacgatg attgtctaca gttgcaggtg
    15781 gatttgaata tactactagt tatttgttgt ctaggataat agatttagta tgttgataag
    15841 tttgactcag atttgtattt tctaataaat gataactcac gatatcgatt aaaaagagtg
    15901 tcgcaatttg tgtgttgata aattgatggt cggtattacg cgattgatcc gttgttaaaa
    15961 gtactaaatt tgcacaatct gtaagtttac taccttcgaa atttgtgatg gcaacgacat
    16021 atgcaccatg agatttggcg acttccgctg ctgaaattaa ttccgaagta ttaccactat
    16081 ttgacatagc aataaacatg tccgaatgag atagtaggga tgccgatatt ttcattaaat
    16141 gtgaatcggt agtaacatta ccttttagcc ccatacgaat catacgataa taaaattcag
    16201 tcgctgataa accagagcta cctagtccag caaagagtat atgtcgactt gattggagtt
    16261 tgtcgataaa ggtttggata atgtcgttat caataaattc accagtttgt tgaatgattt
    16321 gttgatgata tttatgaatt ctttgaataa ttgggctatt ttcaataact gtctctgtca
    16381 tttcttgttg aatattaaat tttaaatctt ggaaattctc ataatctagc ttatgactaa
    16441 agcgtgtcat cgttgctggt gatgtaccaa tcgcatgggc taaggagtta atcgttgaaa
    16501 aggcatcgct ataaccattt tgtcttatat aattgacgat gcgtttatca gtttttgtaa
    16561 ataaatgttg ataacgttga acacgattct caaatttcat tgtgtcaccc cttcatctta
    16621 atgattacta ttatatatga aaaatatttt caagatagta aaaagcattg ataaaaatta
    16681 tcttaatgat atattgtaaa tgactttacg tgaaaaaacg acttatggag tgaggaataa
    16741 tgttaccaca tggattaata gtatcttgtc aggcactagc agatgaacca ttgcattcat
    16801 cttttattat gtcgaaaatg gcattagctg cgtatgaagg tggtgctgtt ggtattcgcg
    16861 caaatactaa ggaagacatt ttagcaatta aagaaacggt agatttacca gttattggca
    16921 ttgtgaaacg tgactatgat cactcagatg ttttcattac tgcaacatca aaagaagttg
    16981 atgaactgat agaaagccaa tgtgaagtca ttgcattgga tgcaacgtta cagcaacgtc
    17041 cgaaagaaac gttagacgaa ttagtatcat atattagaac acatgcaccg aacgttgaaa
    17101 tcatggctga tatcgcgacc gttgaagaag ctaaaaatgc cgcacgactt ggctttgatt
    17161 atattggcac gacgttacat ggctatacta gttatacgca aggacaatta ctttatcaaa
    17221 atgacttcca atttctaaaa gatgtactac aaagtgttga tgcaaaagtt attgcggaag
    17281 gtaatgtcat tacaccggat atgtataaac gcgtgatgga cttaggcgtt cattgttcag
    17341 tcgttggtgg tgcgataaca cgaccaaaag aaattacgaa acgttttgtt caagttatgg
    17401 aagattaaat gataacgata aaaaaacgag atgaccatca ttaattaaag gtacctaatt
    17461 atcttaggtg gctgaatgaa tgtaatgggt tcatctcgtt ttgtttgttt acgatagtga
    17521 ttttattttc aatttgaatt atccaaaaat aagtaaagcg acggggatgg tgattaatag
    17581 cgacaatgcc acgcgtaaaa accaaatgat gatgagtttc cagacaggta ttttaatttc
    17641 agttgctagt atacatggca ctaatgctga gaaaaagata atggctgata cgcttactac
    17701 accgacgaca aatttagtac tcattgcagc tttagttact aacaaagatg gtagaaacat
    17761 ttctacaata gaaatcgctg acgcttttgc aagtacagcc tgatcagcaa ttgggaaaat
    17821 ataaataaat ggatagaata tatagccaag ccaatcaatg aatggtgtat agttcgctac
    17881 aatcagtcct aaaaaaccaa tcgataatat agaaggcaaa ataccaacag tcatttctaa
    17941 accgtcttta aaattgtccc aaacgttctt cacgagagat ggtgttaatg cattttgttt
    18001 catcgcctct gcatatgcag ttttcagtct gcttccttca atagcaactt cttgttctcc
    18061 ttcttgtccg ttataatatt ctgttgattc attggtgatt ggcggtagcc atgcagtaat
    18121 tgcagtcacg acaaatgtga tgactaaagt tatccaaaag tataaattcc aatgcggcat
    18181 tagccctaaa gttttagcaa cgataatcat aaaagttgct gaaactgttg aaaagccagt
    18241 cgcaataatc gtggcttctc gtttgttgta catcccttgc ttatagacac gattagtaat
    18301 caataatcct aaggaataac tgccgacaaa cgaagctact gcatcgacag cggattttcc
    18361 tggtgtttta aaaataggtc tcataatagg ctccatataa acaccgacaa attctaataa
    18421 gccatagcct actaataaag aaagcgcaat tgcacctact ggaattaaga tacttaatgg
    18481 catcattaat ttttcaaaca agaacggacc atagttagct ttaaatagta ttgatggacc
    18541 gattttaaat acatacatta taccaatcat tgcacctgca actttaaata atgtaatgac
    18601 caagtttgtg attgaagtca taaaagtacg tctcactatt ggtaacgctg taccaattaa
    18661 aatcataatc agtgcaacat agggcataag tggacctatg attgagcgaa tggctagatg
    18721 aacatgatcg acgaaaatag tgttgttacc attaatcgta aaaggaataa agaaacatag
    18781 tatgcccact aaactataga caaaaaaacg ccatgcactt ggttgttgtg cattagaatg
    18841 atattgattc attaaagcaa cccctttgtt taaatgaata cacaaaactg tatgatgcat
    18901 cttcccctta atgagatgaa tcattatttt aatttagaaa aatctgaaaa cttactataa
    18961 ttgtatagtt tgaattattt tca
//
LOCUS       DAHHSC010000031        18809 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.031, whole genome shotgun sequence.
ACCESSION   DAHHSC010000031 DAHHSC010000000
VERSION     DAHHSC010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 18809)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18809)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18809
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.031"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            <1..284
                     /locus_tag="NBI57_000847"
     CDS             <1..284
                     /locus_tag="NBI57_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001591193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCD1821811.1"
                     /translation="ILIPLLGLTYMTQSILNIAIITLRQKKCSENMLGRVNSVFKTMV
                     FAFRPIGLFLGGILLENKGGFYALTISAVLFIPLVLYILKNRFYQVEEY"
     gene            751..1122
                     /locus_tag="NBI57_000848"
     CDS             751..1122
                     /locus_tag="NBI57_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCD1821812.1"
                     /translation="MEKLNQVMLYVDDVEKAKAFWTETLEFVVVSETPLAEDYVAVEV
                     SPTKDAETSLTIMAKEFIEKHSPEVNLGTPSLMFKEKNFDALYSKLNDLGLTGHDIVE
                     MDGQRVFNFQDGQGNYFAVSD"
     gene            1061..1315
                     /locus_tag="NBI57_000849"
     CDS             1061..1315
                     /locus_tag="NBI57_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1821813.1"
                     /translation="MVNVYLISKMDKVIILQLVTKQSILIFIQTFILIFGLNFSMKVL
                     FLSFYAIVCTNLLTFLERVTKLLLYRGWKRFPMQKLTGGD"
     gene            1316..2335
                     /locus_tag="NBI57_000850"
     CDS             1316..2335
                     /locus_tag="NBI57_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCD1821814.1"
                     /translation="MVTIKDVALKAGVSPSTVSRVIKGNKRISEATISKVKKVMEELN
                     YFPNTAARTLITNQTYKIGLVLKGSEEPIRLNPFYINVLLGISETCNQHGYGTQTTVS
                     NNMNDLMDEVYKMIKQRMVDAFILLYSKENDPIKQMLIDESMPFIVIGKPTSDIDHQF
                     THIDNDNILASENLTRHVIEQGVDELIFITEKGNFEVSKDRIQGFETVASQFNLDYQI
                     IETSNEREVILNYMQNLHTRLKDPNIKQAIISLDAMLHLAILSVLYELNIEIPKDVMT
                     ATFNDSYLTEIASPPQTCIDIKPRMLGQQAGSAILNILKNKAQDVIELVIIDTELKIR
                     KSTQR"
     gene            2351..4000
                     /locus_tag="NBI57_000851"
     CDS             2351..4000
                     /locus_tag="NBI57_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HCD1821815.1"
                     /translation="MNKQWWKEAVAYQVYPRSFNDSNHDGIGDLPGMIDKLDYLKDLG
                     IDVIWLSPMFKSPNDDNGYDISDYQEIMDEFGTMEDFDCLLKGIHDRGMKLILDLVVN
                     HTSDEHPWFIESKSSKDNPKRDWYIWQDPKPDGSEPNNWESIFNGSTWEYDANTEQYY
                     FHLFSKKQPDLNWGNPEVRDAVFEMMNWWFDKGIDGFRVDAITHIKKTFEAGDLPVPE
                     GKTYAPAFDVDMNQPGIQTWLQEMKDRSLSKYDIMTVGEANGVSPDDADDWVGEENGK
                     FNMIFQFEHLGLWNSGDSHFDVNSYKSVLNRWQKQLENKGWNALFIENHDQPRRVSTW
                     GDDDKYWYESATSHAAVYFLQQGTPFIYQGQEIGMTNYPFESIETFNDVAVKNDYQIV
                     KAQGGDVDALLAKYKDENRDNSRTPMQWDDTLNGGFTNGEPWFPVNLNYKTINVAQQL
                     EDEHSVLQFYKDLIQLRKSNDVYVYGQFDLVDAENSQVFAYTRTLNEKQVLIVGNLTN
                     HEAELTVPFDLSHGEVKLFNYDAKVNLKQLRPYEACVIELN"
     gene            complement(4082..4948)
                     /locus_tag="NBI57_000852"
     CDS             complement(4082..4948)
                     /locus_tag="NBI57_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014373838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HCD1821816.1"
                     /translation="MDVIKQIQQAIVYIEDRLLEPFNLQELSDYVGLSPYHLDQSFKM
                     IVGLSPEAYARARKMTLAANDVINGATRLVDIAKKYHYANSNDFANDFSDFHGVSPIQ
                     ASTKKDELQIQERLYIKLSTTERAPYPYRLEETDDISLVGYARFIDTKYLSHPFNVPD
                     FLEDLLIDGKIKELRRYNDVSPFELFVISCPLENGLEIFVGVPSERYPAHLESRFLPG
                     KHCAKFNLQGEIDYATNEAWYYIESSLQLTLPYERNDLYVEVYPLDISFNDPFTKIQL
                     WIPVKQSPYDED"
     gene            complement(5178..6662)
                     /locus_tag="NBI57_000853"
     CDS             complement(5178..6662)
                     /locus_tag="NBI57_000853"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HCD1821817.1"
                     /translation="MSTKNKHIPCLITIFGATGDLSHRKLFPSIFHLYQQDNLDEHIA
                     IIGIGRRDITNDDFRNQVKSSIQKHVKDTNKIDAFMEHVFYHRHDVSNEESYQELLDF
                     SNELDSQFELKGNRLFYLAMAPQFFGVISDYLKSSGLTDTKGFKRLVIEKPFGSDLKS
                     AEALNNQIRKSFKEEEIYRIDHYLGKDMVQNIEVLRFANAMFEPLWNNKYISNIQVTS
                     SEILGVEDRGGYYESSGALKDMVQNHMLQMVALLAMEAPISLNSEDIRAEKVKVLKSL
                     RHFQSEDVKKNFVRGQYGEGYIDGKQVKAYRDEDRVADDSNTPTFVSGKLTIDNFRWA
                     GVPFYIRTGKRMKSKTIQVVVEFKEVPMNLYYETDKLLDSNLLVINIQPNEGVSLHLN
                     AKKNTQGIETEPVQLSYSMSAQDKMNTVDAYENLLFDCLKGDATNFTHWEELKSTWKF
                     VDAIQDEWNMVDPEFPNYESGTNGPLESDLLLARDGNHWWDDIQ"
     gene            6975..7895
                     /gene="rnz"
                     /locus_tag="NBI57_000854"
     CDS             6975..7895
                     /gene="rnz"
                     /locus_tag="NBI57_000854"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HCD1821818.1"
                     /translation="MEVTFFGTSAGLPTKERNTQAIALNLEPYSNSIWLFDVGEGTQH
                     QILHHAIKLGKVTHIFITHMHGDHIFGLPGLLSSRSFQGGEQKPLTLVGPKGIKAYVE
                     MSMNLSESHLNYPITYIEIDDHLTYHHDGFTVEAHLLNHGIPSYGYRVMAPETTGTIN
                     VEALKNIGLEPGPKYQEVKSHDTFEHNGQVYQSKDFRGESKQGPVVAIFGDTKPCSNE
                     RVISRDADVMVHEATYIDGEKHLANNYHHSHIEDVFALIKEANVKRTLITHLSNRYNT
                     EDINEIYQILIQNEDTPNFNFVKDFDSFKV"
     gene            complement(7998..8813)
                     /gene="proC"
                     /locus_tag="NBI57_000855"
     CDS             complement(7998..8813)
                     /gene="proC"
                     /locus_tag="NBI57_000855"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002485765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HCD1821819.1"
                     /translation="MKLVFYGAGNMAQAIFTGIINSSNLDANDIYLTNKSNEQALKAF
                     AEKLGVNYSYDDATLLKDADYVFLGTKPHDFDALATRIKPHITKDNCFISIMAGIPID
                     YIKQQLECQNPVARIMPNTNAQVGHSVTGISFSNNFDPKSKDEINDLVKAFGSVIEVS
                     EDHLHQVTAITGSGPAFLYHVFEQYVKAGTKLGLEKEQVEESIRNLIIGTSKMIERSD
                     LSMAQLRKNITSKGGTTQAGLDTLSQYDLVSIFEDCLNAAVDRSIELSNVEDQ"
     gene            8955..9710
                     /locus_tag="NBI57_000856"
     CDS             8955..9710
                     /locus_tag="NBI57_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCD1821820.1"
                     /translation="MKGQHFIVTGGTSGLGLSIVRKLLENKVHVTLLVRDVDKATRIF
                     EQELGKTINVIPCDLNDLKSIQALQFEDNTSFDGFIYSAGLGYFKSISDHSFSEMIET
                     YQLNLISFNVLYTVLRPYLTSNAHIVGISSQAAFSTQANAAHYGASKAGFYALMNALR
                     LESPNLHIMTVNVGPIDTPFHQKADPSMKYAKKMGEIMLDANQLAEDIIYGIKTKQLE
                     INRPKWMHHALKMYQIAPRFFERCFPKLFKNKA"
     gene            9726..9974
                     /locus_tag="NBI57_000857"
     CDS             9726..9974
                     /locus_tag="NBI57_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1821821.1"
                     /translation="MNCQSESVVRLCVRYAEQLSVFEEFTVLDILGDISVDQISDGTL
                     YYTCEKFKLLVLQGNVLGVQIITNNDESTCEVKYRKMF"
     gene            complement(10190..11098)
                     /locus_tag="NBI57_000858"
     CDS             complement(10190..11098)
                     /locus_tag="NBI57_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HCD1821822.1"
                     /translation="MQKNILKSGIALSELGLGCMSLGTDLKKAEQIIDCAVENGITYF
                     DTADMYDKGINESVVGKALLKYQQRDDIFIGTKVGNRLTKDGSTTWDPSKSYIKEAVK
                     GSLKRLGIDHIDLYQLHGGTIDDPLDETISAFDELKQEGIIRAYGISSIRPNVIDYYL
                     KHSQIETIMSQFNLIDNRPESLLDAIHNNDVKVLARGPVSKGLLTSNSVNVLDNKFKD
                     GIFDYSHDELGETIASIKEIESNLSALTFSYLTSHDVLGSIIVGASSVDQLKENIENY
                     HTKVGLDQIKTARARVKDLEYTNHLV"
     gene            11180..11722
                     /locus_tag="NBI57_000859"
     CDS             11180..11722
                     /locus_tag="NBI57_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HCD1821823.1"
                     /translation="MDLNEKTIDRTVIYNGKIVDVEIHTVTLPNGETSTRELVYHNGA
                     VAVCALTPKKEVVLVKQYRKPVEKPLLEIPAGKLEDDENRVEAAKRELEEETGYIAKE
                     LTHVVDMYGSPGFCDEQLSIYFTDNLEEGTVHLDEDEFVEVIKVPIENVKSMLMNKEI
                     EDAKTIIALQHLLLNYNHSK"
     gene            11827..12276
                     /locus_tag="NBI57_000860"
     CDS             11827..12276
                     /locus_tag="NBI57_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HCD1821824.1"
                     /translation="MEERLNRVKQQLQQSSYKLTPQREATVRVLIENEKDHLSAEDVY
                     LKVKDKAPEIGLATVYRTLELLAELKVVDKINFGDGVARFDLRKEGAKHFHHHLVCME
                     CGRVDEIDEDLLPEVENRVENEFNFKILDHRLTFHGVCETCQAKGKG"
     gene            12325..13212
                     /gene="xerD"
                     /locus_tag="NBI57_000861"
     CDS             12325..13212
                     /gene="xerD"
                     /locus_tag="NBI57_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HCD1821825.1"
                     /translation="METIIEEYLRFIQIEKGLSSNTIGAYRRDLKKYQDYMTEHHISH
                     IDFIDRQLIQECLGHLIDQGQSAKSIARFISTIRSFHQFAIREKYAAKDPTVLLDSPK
                     YDKKLPDVLNVDEVLALLETPDLNKINGYRDRTMLELLYATGMRVSELIHLELENVNL
                     IMGFVRVFGKGDKERIVPLGDAVIEYLTTYIETIRPQLLKKTVTEVLFLNMHGKPLSR
                     QAIWKMIKQNGVKANIKKTLTPHTLRHSFATHLLENGADLRAVQEMLGHSDISTTQLY
                     THVSKSQIRKMYNQFHPRA"
     gene            complement(13290..13796)
                     /locus_tag="NBI57_000862"
     CDS             complement(13290..13796)
                     /locus_tag="NBI57_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF309 domain-containing protein"
                     /protein_id="HCD1821826.1"
                     /translation="MQQALINFYYQFHTKQHYFLCHDILEDAWKAENNYSKQDAVVSL
                     ILFATACYHYRRNNLKGAYKSFNKSKEIIQNAKDRDALYLNLNDYQLLIEQQIAKLNA
                     AKPFSSVILPITPVFERIIKANYPDYDYNQETATDPFIVDHHMRRDRSEVIAAKEEAI
                     QLRKHRRN"
     gene            13888..14619
                     /locus_tag="NBI57_000863"
     CDS             13888..14619
                     /locus_tag="NBI57_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HCD1821827.1"
                     /translation="MYEVKLDAFNGPLDLLLHLIQKFEIDIYDIPMQALTEQYMQYVH
                     AMKQLEINIASEYLVLASELLMIKSKMLLPQSTSDMDVDDDPREDLVGRLIEYQNYKE
                     YTAILNDMKEERDFYFTKRPTDLSHLETDESWDPNHTIDLTELIVAYQRVKNRVELNT
                     PKSVEIRKETFTIQQATEQVTSRLKDKDHFNFFSLFTFSEPIEQVVTHFLAILEMSKA
                     GIINIEQQRNFEDINIIRGVNYHFG"
     gene            14612..15154
                     /gene="scpB"
                     /locus_tag="NBI57_000864"
     CDS             14612..15154
                     /gene="scpB"
                     /locus_tag="NBI57_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HCD1821828.1"
                     /translation="MDNHGILESLLFTAGDEGLDEKQLLEILDMSKDQLVELIENYSS
                     HGLMIQRFGTTYVLTTKKEAATYIEQLIEQKSQMKLSQAAMEVLSIIAYNQPLSRSDI
                     ELIRSINSDGAVKTLIAKGLVEAKVVNEQRSQQLITTDLFLNVFGISNIEDLPTTEED
                     DEEMDAFFSNLVNQKGENND"
     gene            15147..15884
                     /locus_tag="NBI57_000865"
     CDS             15147..15884
                     /locus_tag="NBI57_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HCD1821829.1"
                     /translation="MTKELERLQKRIANSGYTSRRKAETLISEGKVKVNGTTVTELGT
                     KVKPSDTIEVEGIKIELEDKIYILFHKPTQVITSVSDDRGRTVVTDYFKDIEARIYPV
                     GRLDYDTSGLLLLTNDGEFTNLMTHPRYQIKKKYVAKLKGYLMREEVKALEKGIELED
                     GMTQPAEVKVKKQDKDKNTTLVEITITEGRNRQVRRMFEHFGHQVTKLSRIEYGPLNV
                     VGLNAGEGRVLTPHEVKVMRHLAEHGK"
     gene            16017..16742
                     /gene="srrA"
                     /locus_tag="NBI57_000866"
     CDS             16017..16742
                     /gene="srrA"
                     /locus_tag="NBI57_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator SrrA"
                     /protein_id="HCD1821830.1"
                     /translation="MSNEILIVDDEDRIRRLLKMYLERESFEIHEASNGQEAYELAME
                     NNYACILLDLMLPEMDGIQVATKLREHKQTPIIMLTAKGEETNRVEGFESGADDYIVK
                     PFSPREVVLRVKALLRRTQSTTVEQSEPHARDVIEFKHLEIDNDAHRVLADNQEVNLT
                     PKEYELLIYLAKTPNKVFDREQLLKEVWHYEFYGDLRTVDTHVKRLREKLNRVSSEAA
                     HMIQTVWGVGYKFEVKSNDEPAK"
     gene            16723..18474
                     /gene="srrB"
                     /locus_tag="NBI57_000867"
     CDS             16723..18474
                     /gene="srrB"
                     /locus_tag="NBI57_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     SrrB"
                     /protein_id="HCD1821831.1"
                     /translation="MMSRLNSVVIKLWLTIILIVTTVLILLSIALITFMQYYFTQETE
                     NAIREDARRISSLVEQSHNKEEAIKYSQTLIENPGGLMIINNKHRQSTASLSNIKKQM
                     LNEVVNNDHFDDVFDKGKSVTRNVTIKEKGSSQTYILLGYPTKAQKNSHSKYSGVFIY
                     KDLKSIEDTNNAITIITIITAVIFLTITTVFAFFLSSRITKPLRRLRDQATRVSEGDY
                     SYKPSVTTKDEIGQLSQAFNQMSTEIEEHVDALSTSKNIRDSLINSMVEGVLGINESR
                     QIILSNKMANDIMDNIDEDAKAFLLRQIEDTFKSKQTEMRDLEMNARFFVVTTSYIDK
                     IEQGGKSGVVVTVRDMTNEHNLDQMKKDFIANVSHELRTPISLLQGYTESIVDGIVTE
                     PDEIKESLAVVLDESKRLNRLVNELLNVARMDAEGLSVNKEVQPIAALLDKMKIKYRQ
                     QADDLGLNMTFNYCKKRVWSYDMDRMDQVLTNLIDNASRYTKPGDEIAITCDENESED
                     ILYIKDTGTGIAPEHLQQVFDRFYKVDAARTRGKQGTGLGLFICKMIIEEHGGSIDVK
                     SELGKGTTFIIKLPKPE"
BASE COUNT         6630 a         2698 c         3380 g         6101 t
ORIGIN      
        1 ttatactcat cccactttta gggttgacat atatgacaca atccatttta aatatagcga
       61 taatcacact gaggcaaaag aaatgttcag aaaatatgtt aggacgtgtt aatagtgttt
      121 ttaaaacaat ggtttttgca ttcagaccta taggcctttt tttaggcggt atattacttg
      181 aaaataaagg tggattttat gcacttacga tcagtgctgt gttatttata ccattagtgt
      241 tatatatact gaaaaataga ttttatcaag ttgaagaata ttaagaagaa aattaataaa
      301 aatggctgct atgtcaaatt ggactgatga attcaatata ggaagttagg caactaaaca
      361 ttgtataact ttctttttag ttattggagc ttaaagtttt gaacataata atattcgatt
      421 gcgcaaatga ttgtaacttc cataatcaaa tgatgtacgt ttaattaatt ttattatgtt
      481 agttatacat tctaatggac aatgtgagct acacccagtt aagttgacat agaaaatacg
      541 aaaactttga acgaagtatt tcattgatat atgtcccaga cgacatgttt gattaatgaa
      601 gttctatgtg gctacaaaat tttaaaaatt acacaagcaa tatctattac cttaaaagca
      661 tcccgccata tttttaagca ttttgtgaag taataaaata tttactgtgt tatgatagca
      721 tgtactatag agaaatagag gtactatttt atggaaaagt taaatcaagt tatgttatat
      781 gttgatgatg ttgaaaaagc gaaagcattt tggacagaaa ctttagaatt tgttgttgta
      841 agtgaaacac cgttagctga ggactatgtt gcagttgaag tatcacctac caaagatgct
      901 gaaacgtcat tgacgattat ggcaaaagaa tttattgaaa agcatagtcc agaggtgaac
      961 ctaggaacac catctttaat gtttaaagag aaaaactttg atgcattata ctcaaaatta
     1021 aatgatttag gtttaactgg gcatgatata gtagaaatgg atggtcaacg tgtatttaat
     1081 ttccaagatg gacaaggtaa ttattttgca gttagtgact aaacaatcga ttttaatatt
     1141 tatacaaacc tttatattga ttttcggatt gaatttcagt atgaaggttt tatttttatc
     1201 gttttatgca atcgtttgca caaatctttt gactttttta gaaagagtaa ctaaactgtt
     1261 attatataga ggttggaagc gttttccaat gcagaaatta acaggaggtg attaaatggt
     1321 tacgattaaa gatgttgcac taaaagccgg tgtttctcct tcgacagttt caagagttat
     1381 aaaaggaaat aaacgtatta gcgaagcgac aatttcaaaa gtgaagaaag ttatggaaga
     1441 attgaattat tttcctaata ccgctgctag aactttaatt acaaaccaaa catataaaat
     1501 tggtttagtg ttaaaagggt ctgaggagcc tattcgactg aatccattct acatcaatgt
     1561 attgctagga atttctgaaa cgtgtaacca gcatggctat ggtacacaaa cgacagtctc
     1621 aaataatatg aatgatttaa tggatgaagt ttataaaatg attaaacaac gaatggttga
     1681 tgcgtttata ctgctttatt caaaagaaaa tgatccaatt aaacaaatgt taattgatga
     1741 aagcatgcca tttattgtga ttggtaagcc tacatcggat atagatcatc aatttacaca
     1801 catagataat gataatatat tagcttctga aaatttgaca cgacatgtta ttgaacaagg
     1861 tgtagatgaa ttaatattta ttacagaaaa aggaaatttt gaagtttcaa aagatagaat
     1921 tcaaggattt gaaacggttg catcacaatt taatctggat tatcaaatta ttgagactag
     1981 taatgaaaga gaagttattt taaattacat gcaaaatcta catacgcgtt tgaaggaccc
     2041 aaatattaaa caggcaatca tttcgttaga tgctatgtta catttagcga ttttaagtgt
     2101 cctatatgaa cttaatattg aaattccgaa agatgtaatg acagcaacgt tcaatgattc
     2161 ttatttaacc gaaattgcgt caccacctca aacctgtatt gacatcaagc ctcgaatgtt
     2221 aggtcaacag gctggttcag caattttaaa tatattgaaa aacaaagcac aggatgttat
     2281 tgaactcgtc attatagata cagaattaaa aataagaaaa tcaacacagc gatagcaaag
     2341 gagttttaaa atgaataagc aatggtggaa agaagcagta gcatatcaag tatatccaag
     2401 aagttttaat gatagtaatc acgatggtat tggggattta cctggaatga ttgataaatt
     2461 ggactactta aaagatttag gtatcgatgt catttggctc agtccaatgt ttaaatcacc
     2521 taatgatgac aatggttatg atattagtga ctatcaagaa attatggatg aatttggaac
     2581 gatggaagac tttgattgtt tattaaaagg tattcatgat agaggcatga agcttatttt
     2641 agatttagtt gtaaatcata catctgatga acatccttgg tttatagaat ctaaatctag
     2701 taaagacaat cccaaacgtg attggtatat ttggcaagat ccaaagccag atggctctga
     2761 acctaacaac tgggaaagta tctttaatgg atctacatgg gaatatgatg ctaatactga
     2821 gcaatattat ttccatttat tcagtaaaaa acaacctgat ttgaattggg gtaatccaga
     2881 agttagagat gctgtatttg aaatgatgaa ctggtggttt gataaaggca ttgatggatt
     2941 tagagtagat gcaattacgc atattaagaa gacgtttgaa gcgggtgact tacctgtacc
     3001 tgagggtaaa acatatgccc cagcatttga tgtagatatg aatcagccag gtatacaaac
     3061 ttggttacaa gagatgaaag atcgctcatt aagtaagtat gacattatga ctgtcggtga
     3121 agcgaatggt gtaagccctg atgatgctga tgactgggtc ggggaagaaa atggtaaatt
     3181 taatatgata ttccaatttg aacatttggg actgtggaat agtggtgatt ctcactttga
     3241 tgtaaattcg tataaatctg tattaaatag atggcaaaaa caacttgaaa ataaaggttg
     3301 gaatgcgttg tttattgaaa atcacgacca accacgacgt gtatcgacgt ggggtgacga
     3361 tgacaagtat tggtatgaat cagcaacaag tcatgcagct gtttatttct tgcaacaagg
     3421 tacgccattc atttatcaag gtcaagaaat tggtatgacg aattatccat ttgaaagtat
     3481 tgaaacgttt aacgatgttg ctgtaaaaaa tgactatcaa atagtgaaag ctcaaggtgg
     3541 agatgtagac gctttacttg cgaaatataa agatgagaac cgagataatt ctcgcacacc
     3601 aatgcaatgg gatgatacgt taaatggagg atttacaaat ggtgaaccgt ggttcccagt
     3661 gaatctgaat tataaaacta tcaatgttgc acaacaatta gaagatgagc attcagtatt
     3721 acaattttat aaagatttaa ttcaattaag aaagtctaat gatgtatacg tatatggtca
     3781 atttgattta gtagatgctg aaaattcaca agtttttgcg tacacgagaa cattaaatga
     3841 aaagcaagtt cttattgtag gtaatcttac taaccacgaa gcagaattaa ctgtaccatt
     3901 tgatttaagc catggagaag taaagctatt taattatgat gccaaagtta atttaaaaca
     3961 gttacgtcca tatgaagcat gtgttatcga actaaattaa catattaata tgccccctaa
     4021 tagaatctaa ggccatttgt agacctatag atatattagg gggcttcttt gtttttaatt
     4081 tttaatcttc gtcataagga ctctgtttaa caggaatcca aagctgaatt ttagtgaatg
     4141 ggtcattaaa tgaaatatcg agagggtaca cttcaacata taaatcattt cgttcatatg
     4201 gcaatgttaa ctgcaaactt gattcaatat agtaccaagc ttcattagtt gcataatcaa
     4261 tttcaccttg taaattgaat ttcgcacaat gtttgccagg taaaaatcgg ctttctaagt
     4321 gtgcaggata acgttcactt ggtacaccta caaatatttc taaaccattt tcaagaggac
     4381 aactaataac aaatagttca aatggactaa cgtcattata tcgtcgtaac tctttaattt
     4441 taccatcaat gagcaagtct tctaaaaaat ccggaacatt aaaaggatgt gacaaatact
     4501 tagtgtctat aaatcgtgca tatccaacca atgaaatatc atctgtctct tctaatctgt
     4561 atggataggg tgctctctca gtagttgata atttgatata taatcgctct tgaatttgta
     4621 attcatcttt tttagtagaa gcttgaatag gtgatacgcc gtgaaaatca ctaaaatcat
     4681 ttgcaaaatc atttgaattt gcatagtgat attttttagc gatatctaca agtcgtgtag
     4741 caccattaat cacatcattt gcagcgagtg tcattttacg cgcgcgtgca taagcttctg
     4801 gagataagcc gacaatcatt ttaaatgatt gatcaagatg gtatggcgaa agaccaacgt
     4861 aatcacttaa ttcttgcaaa ttgaaaggct ctaataaacg atcttcaata taaacaattg
     4921 cctgttgtat ttgcttgata acgtccaaaa ctttcactcc aattaattca atcatttatt
     4981 tttattctac attatttcta taaattatac acccatttgt tcaatgatta ttaaaatagt
     5041 tttgggcatt gtaaaatata atttcataat atagtctaga aaaaaagcga atgatagaac
     5101 aattgattta cttgattcgt aatcaatcct tgtcattcgc tcatttattt ttgtttaaca
     5161 tgtgcgtttt aattcaatta ttgaatatcg tcccaccaat ggttaccatc acgagcaagt
     5221 agtaaatcac tttctaatgg accattagta cctgattcat agttagggaa ttctggatca
     5281 accatattcc attcatcttg aattgcatca acaaatttcc atgttgattt taattcttcc
     5341 cagtgcgtga agttagtggc atcaccttta agacaatcga ataatagatt ttcatatgca
     5401 tctacagtat tcattttatc ttgagcgctc attgaataag acaattgaac aggttcagtt
     5461 tcgatacctt gtgtattttt cttagcattt agatgtaaag atacaccttc attaggttgg
     5521 atattgatta ctaataggtt tgaatctaat agtttatcag tttcatagta taagttcatt
     5581 ggtacttctt taaattcaac gacaacttga attgttttag atttcatacg tttaccagta
     5641 cgaatataga atggtacacc agcccatcta aagttatcaa ttgttaattt acctgaaaca
     5701 aaggtaggtg tgttagagtc atctgcaacg cgatcttcat cacggtatgc tttaacttgt
     5761 ttaccatcga tatagccttc gccatattga ccacgaacaa agttcttttt aacatcttca
     5821 gattggaaat gacgcagtga tttaagtact tttactttct cagcacggat atcttcactg
     5881 tttaaactaa taggtgcttc catagctaat aatgcaacca tttgtaacat gtggttttgc
     5941 accatatctt ttagcgcgcc acttgattca taataaccac cacgatcttc aacacctagt
     6001 atttcagaag atgtaacttg gatgtttgaa atatatttgt tattccataa tggttcaaac
     6061 atcgcattcg caaaacgtaa tacctcgata ttttgaacca tgtcttttcc taaatagtgg
     6121 tcaatacgat aaatttcttc ttctttaaat gatttacgaa tttgattgtt taatgcttcg
     6181 gctgatttta aatcactacc gaatggtttt tcgataacaa ggcgtttaaa tccttttgta
     6241 tcagtaagac cagaagattt tagataatca gaaataacgc caaagaattg tggtgccatt
     6301 gctaaataga atagtcgatt accttttaat tcaaattggc tatctaattc attactaaaa
     6361 tctagtaatt cttgatagct ttcttcatta ctaacatcat gtctatgata gaagacatgt
     6421 tccataaacg cgtcaatttt gtttgtatct tttacgtgct tttgaattga tgattttact
     6481 tgattacgga aatcatcatt agtaatgtca cgacgtccaa taccgatgat ggcaatatgt
     6541 tcatctaaat tgtcttgttg gtagagatgg aatattgatg gaaacaactt acgatggctt
     6601 aagtcaccag ttgcaccaaa gattgtgatt aaacaaggga tgtgtttgtt tttagtactc
     6661 aagattaaaa cctcaattct taattagata tatgatttat tattataaga taatccattc
     6721 agcaggtcat tcaatatgct cgacagtatg cagtgtctta aatgaaatat cgattcatgt
     6781 atcatgttta atgtgatact tattaatgat aagtataacg taattgtcaa aatttatata
     6841 gttatgtcta cgttaaagtt agaaaaatta actagcaaag acgaattttt aacagatttt
     6901 gattcaagta taaattaaaa ctaaattgat acaaatttta tgataaaatg aattgaagaa
     6961 aaggaggggc atatatggaa gttacatttt ttggaacgag tgcaggtttg cctacaaaag
     7021 agagaaatac acaagcaatc gccttaaatt tagaaccata ttccaattcc atatggcttt
     7081 tcgacgttgg tgaaggtaca cagcaccaaa ttttacatca tgcaatcaaa ttaggaaaag
     7141 tgacacatat atttattact catatgcatg gcgatcatat ttttggtttg ccaggattac
     7201 tttctagtcg ttcttttcag ggcggtgaac agaagccgct tacattggtt ggaccaaaag
     7261 gaattaaagc atatgtggaa atgtctatga atttatcaga gtcacattta aactatccaa
     7321 ttacatatat tgaaatcgat gatcatctga catatcatca tgatggtttt actgtagagg
     7381 cgcatttact taatcatggt attccatcat atggatatag agtaatggca cctgaaacta
     7441 caggtacaat caatgttgaa gcgttaaaaa acattggtct tgaaccaggt cctaaatatc
     7501 aagaagtaaa atctcatgat acatttgaac ataatggtca agtttatcaa tcaaaagatt
     7561 ttagaggtga gtccaaacag ggtccagttg tagcaatttt tggtgataca aaaccatgtt
     7621 ctaatgaacg tgtgatttct agagatgctg atgtgatggt acatgaagcg acctatatag
     7681 atggtgaaaa gcatttagcc aacaattatc atcatagcca tattgaagac gtatttgcat
     7741 tgataaaaga agccaatgtt aaacgtacgt tgattacaca tttgagcaat cgctataata
     7801 cagaagatat aaatgaaatt tatcaaatat tgatacaaaa tgaagataca cctaatttta
     7861 attttgtaaa agattttgat agttttaaag tctaatgaca aagtaataaa caatgcttgc
     7921 caaatagtaa taatttataa aaataacccg tccaactatg tatgtgatga tgcatacatg
     7981 tgttggcggg tttgttttta ttggtcttct acattagaaa gttcaatact acggtcgacg
     8041 gcagcattta gacaatcttc gaaaatagat actaaatcat attgtgacaa tgtatcaagg
     8101 ccagcttgtg tcgtaccacc tttagaggta atattttttc ttaattgagc catgctcaaa
     8161 tctgaacgtt caatcatctt acttgtacct ataataaggt tgcgtataga ttcttcaact
     8221 tgttcttttt ctagaccaag tttcgtacca gctttaacgt attgctcgaa tacatgatat
     8281 aaaaatgctg ggccgcttcc ggtgatagct gttacttgat gtaaatgatc ctctgatact
     8341 tcaattacag aaccaaatgc tttaactaaa tcgttaattt catctttaga tttagggtca
     8401 aagttgtttg aaaaactaat gccagtaact gagtgtccaa cttgcgcatt tgtgtttggc
     8461 ataattctag caactggatt ttggcattct aattgttgtt taatataatc aatcggaata
     8521 cctgccataa ttgaaatgaa gcaattgtct tttgtgatat gtggtttgat gcgtgttgct
     8581 agagcatcaa agtcatgcgg tttggtacct aaaaaaacat aatctgcatc ttttaataat
     8641 gtcgcatcat cataactata gttaacacct agtttttcag cgaatgcttt taaagcttgt
     8701 tcattagatt tatttgttaa atatatatca ttggcatcta agttgcttga gttaataatt
     8761 cctgtaaata tagcttgtgc catattacca gctccataaa atacgagttt cattaacgtc
     8821 acatcccttt agtttttgat ttgtatgtta tattttctta ttattgtata tatattagga
     8881 aagataatca acttgtctca attatacaga gacgataaat tgcattctat ataattgaaa
     8941 aggaaggtta attgatgaaa ggacaacatt ttatagtgac tggtggtacg agtggtttag
     9001 gtttatcgat tgtccgaaag ttactagaaa ataaagtgca tgtaacgttg ttggtgcgtg
     9061 atgttgataa agcaactaga atctttgaac aagaattggg gaaaactata aatgttattc
     9121 cttgtgactt aaatgatttg aagtcgatac aggcactgca atttgaagac aatacatcat
     9181 tcgatggatt catttatagt gcaggtttag gttatttcaa atctatttca gaccatagtt
     9241 tttcagaaat gattgaaacg tatcagctta atttaattag ttttaatgtt ttatatactg
     9301 tgttgagacc atatttaaca tctaacgcac atattgtagg tatttcaagt caagctgcat
     9361 tttccacaca agcaaatgct gcacattatg gggcatcgaa agcagggttt tatgcattaa
     9421 tgaatgcttt aagactagag tcaccaaact tacatatcat gacagttaat gttggcccga
     9481 ttgatacacc ttttcatcaa aaagccgacc catcaatgaa gtatgctaaa aagatggggg
     9541 aaattatgct tgatgctaat cagcttgcag aagatatcat ttatggcatt aaaacaaaac
     9601 aattggaaat taatcggcct aaatggatgc atcatgcgtt gaaaatgtat caaattgcgc
     9661 ctagattttt tgaacgatgt ttccctaaat tatttaaaaa taaagcataa ataggtggga
     9721 tggcaatgaa ctgtcaatcg gagagtgtgg taagactttg tgtacgatat gcggaacaac
     9781 tatcagtatt tgaagaattt acagtgctgg atattttagg cgatatttct gtagaccaaa
     9841 tttcagacgg tacattatat tatacttgtg aaaaattcaa actacttgtg ttgcaaggaa
     9901 atgtgctggg tgtacaaata ataactaata atgatgaatc aacatgcgaa gtgaaatata
     9961 gaaagatgtt ttaataaaat tattaagaga tgaagtggca gaagtttgct taaggcaatg
    10021 ccgcaaggcg aaattactaa atactgatgc gtgaggacaa cgctttaatt tctatcataa
    10081 gtgagtaatc ataatcataa attatagcga attgtacata aacaacagca gtaaggcatt
    10141 tttataatga aacgcctcat gctgctgttt ttattactga aaatgacttc tacactaaat
    10201 gattggtata ttccaaatcc tttacacgag ctcttgctgt tttaatctga tctaaaccaa
    10261 ctttagtatg atagttttca atattttctt ttaattggtc aacgctactt gcacctacaa
    10321 tgatggaacc aagcacgtca tgtgatgtta aataactaaa tgtcaatgca gataaattac
    10381 tttcaatttc tttaatagag gctattgttt cacccaattc atcatgagaa taatcaaaaa
    10441 taccatcttt aaatttattg tcgagcacat taacactgtt tgaagttaat aatcctttag
    10501 acacaggtcc tcttgccaat actttaacat cattgttgtg aattgcatct aataatgatt
    10561 ctggacgatt atcaatcaaa ttgaattgag acattatcgt ttcgatttga ctatgtttta
    10621 aataataatc aattacattt gggcgaatag aagaaatacc gtaagcacgt ataattcctt
    10681 cttgtttcaa ttcatcaaat gcgcttattg tttcgtctaa tgggtcatca atggttccgc
    10741 catgaagttg atataaatcg atatgatcga tacctaaacg ctttagtgaa cctttaactg
    10801 cctctttaat ataggattta ctcggatccc atgttgtact gccatctttt gttaaacgat
    10861 tgcctacttt tgtaccaatg aaaatatcat cgcgttgttg atatttaaga agtgccttac
    10921 caacaactga ttcatttata cctttatcgt acatgtctgc tgtatcaaaa taagtgatac
    10981 cattttcaac agcacaatct ataatttgtt cggctttttt taaatctgta cctaaactca
    11041 tgcaacctag acctaattca gataatgcaa taccactttt taatatattt ttttgcatgt
    11101 ctgttctcct ttcgatacac tttagctaac aagcatagtg tatcaatata taatatgaat
    11161 ttaaaggaac gtgattataa tggatttaaa tgaaaaaaca attgatcgaa cagttattta
    11221 taatggcaaa attgtagatg tagaaattca tacagtgaca ttaccaaacg gtgaaacgtc
    11281 aacaagagaa ttagtttatc ataatggtgc agttgctgtt tgtgcattga cacctaaaaa
    11341 agaagtcgta ttagtgaaac agtatcgtaa accagtagaa aaaccattgc tagaaattcc
    11401 agctggtaaa ttagaagatg atgaaaatag agtcgaagcg gctaagcgtg aattagaaga
    11461 agaaacagga tatattgcca aagagttaac acatgttgta gatatgtatg gttcaccagg
    11521 tttttgcgat gaacaattat caatttattt tactgataat ttagaagaag gtacagttca
    11581 cttagatgaa gatgaatttg tcgaagtcat taaagttccg attgagaatg ttaaatcgat
    11641 gctaatgaat aaagaaatag aagatgcgaa gactatcatt gcattgcaac acctattatt
    11701 aaattataat cattctaaat aacaaatgag aaaagcttgc attttattga gaaaactgtt
    11761 agttttaatt gtgaagtttg aaataatttg taatgatttt aattattagt aggggagtgg
    11821 acatcgttgg aagaacgatt aaatcgcgtt aagcaacaat tacaacaatc atcatataag
    11881 ctaacgccac aacgcgaagc tactgttaga gttctaattg aaaatgaaaa agatcatcta
    11941 agtgctgaag acgtatatct gaaagtaaaa gacaaagcgc ctgaaattgg cttggcgaca
    12001 gtatacagaa cgttagagtt gttagctgaa ctaaaagttg tcgacaaaat taactttggt
    12061 gatggcgtcg ctcgttttga tttaagaaaa gaaggcgcaa aacatttcca ccatcattta
    12121 gtatgtatgg aatgtggtcg tgtagatgaa atcgatgaag atttgttacc agaagttgaa
    12181 aatcgagttg aaaatgagtt caattttaaa attttagatc atcgtttaac tttccatggt
    12241 gtgtgtgaaa catgccaagc taaaggtaaa ggatagtaaa ttgcgtaggt taaattaacc
    12301 ttcgcttttt ttagaggtgt ggttatggaa acaattattg aagaatattt gcgttttata
    12361 caaattgaaa aaggactaag ttctaacaca attggtgctt atagacgtga tttgaaaaag
    12421 tatcaagatt atatgactga acatcatatc tcgcatattg attttataga tcgacaatta
    12481 attcaagagt gtttggggca tttaatagac caagggcaat ctgctaaatc tattgcgcga
    12541 tttatttcaa caatccgtag ttttcatcaa tttgctataa gagaaaaata tgcggcgaaa
    12601 gatccaacgg tattattaga ttcaccaaaa tatgacaaaa aattgcctga cgttttaaat
    12661 gttgacgaag tattggcttt attagaaacg ccagatttaa ataaaattaa tggatatcgt
    12721 gatcgtacga tgttagaact tctgtacgca acgggaatgc gtgtatctga attgatacat
    12781 ttagagttag aaaacgtgaa cttaataatg ggatttgtac gcgtatttgg taaaggcgat
    12841 aaagaaagaa ttgtaccatt aggcgacgca gtcattgagt acttaactac ttatattgaa
    12901 acgattagac cgcaactttt aaaaaagact gttactgaag tcttattttt aaatatgcat
    12961 ggtaaacctt tatcacgaca agcaatatgg aaaatgatta aacaaaatgg cgtaaaggca
    13021 aacattaaaa agacgttaac gccacatacg ttacgccact cttttgcgac acatttattg
    13081 gaaaatggcg cagatttaag agcagtgcaa gagatgttag gtcactctga catatctact
    13141 acccaactct atacacatgt ttcgaaatct caaattagaa aaatgtataa ccaatttcat
    13201 cctagagcat aaagtgaacc ataacttaaa agtcacaata cacatgacta aaaatgtctg
    13261 tgctattgtg gcttttttaa attggttgat taattacgtc tatgttttct taattgaatc
    13321 gcttcttctt ttgctgcaat cacttctgaa cgatcacgcc gcatgtgatg gtctacaata
    13381 aaaggatctg ttgctgtttc ctgattataa tcatagtctg gatagttggc cttgatgatg
    13441 cgttcaaaga ctggagttat tggtaatata acagatgaaa aaggctttgc tgcattcaat
    13501 tttgcaatct gttgctcaat taacaattga taatcattta aattaaggta taacgcatct
    13561 ctatctttag cattttgtat tatttcttta gatttattaa aagacttata ggcgcctttt
    13621 aaattattgc ggcgataatg gtaacaagca gttgcaaaca agattaaact aacaactgca
    13681 tcttgcttac tgtagttatt ttcagctttc catgcatctt ctaaaatgtc atgacatagg
    13741 aaataatgtt gcttagtatg aaattgataa tagaaattta tcagtgcctg ttgcattttg
    13801 ttatcacccc aatttaaaag taagttattt tcatgctata atattttaga gaattatgca
    13861 catatgacgc aatacgaggt agatattatg tatgaagtta aattagatgc tttcaatgga
    13921 ccattagatt tattgctgca tcttatccaa aaatttgaaa tagatattta tgatattcct
    13981 atgcaagcat taacagagca gtatatgcag tacgttcatg caatgaaaca gcttgaaatt
    14041 aatattgcaa gtgaatacct agtattagcg tcagaactct taatgattaa aagtaagatg
    14101 ctattaccac aatcaacatc agatatggat gttgatgatg acccacggga agatttagtt
    14161 gggcgtttaa tagaatatca aaattataaa gaatatactg ctattttaaa tgacatgaaa
    14221 gaagaaagag atttttattt tacaaaaaga ccgacagatt tatctcattt ggaaacagat
    14281 gaatcttggg atccaaatca tacgattgat ttaactgaat taattgtagc ttatcaaaga
    14341 gttaaaaata gagttgagtt aaatacacct aaatctgttg aaatccgaaa agagacattt
    14401 accattcaac aagctacaga acaagtgaca tcgagattga aagataaaga tcattttaac
    14461 ttctttagtc tgtttacgtt ttctgagcca attgaacaag tagtcactca ctttttagct
    14521 attttagaga tgtcaaaagc aggaataatt aatattgagc aacaacgtaa ttttgaagat
    14581 attaacatta ttagaggagt gaactaccat tttggataat catggtatat tagagtcgct
    14641 tttatttaca gctggcgatg aaggtttaga tgaaaaacaa ctattagaaa tattagatat
    14701 gtcgaaagac caactcgttg aattaattga aaattattca tcacatggat taatgataca
    14761 acgatttgga acgacgtatg ttttaacgac taaaaaagaa gcggcaacgt atattgaaca
    14821 attaattgaa caaaagtcac aaatgaaatt atcacaagca gcaatggaag tactatcaat
    14881 tattgcttat aaccagccat tatcaagaag tgatattgaa ttaattcgta gtatcaattc
    14941 agatggtgca gttaagacat tgattgccaa aggactagtt gaggctaaag tggttaatga
    15001 acagcgtagc caacagttaa ttactactga tttattttta aatgtatttg gtatttccaa
    15061 tatagaagat ttgccgacaa ctgaagaaga cgatgaagaa atggatgctt ttttcagtaa
    15121 tctagtcaat caaaaaggag aaaataatga ctaaagaatt agaacgatta caaaaacgta
    15181 tagctaatag cggttatact tcaagacgta aggcagaaac tttaatttct gaaggaaaag
    15241 tgaaagtgaa tggtacgact gtcactgaat taggaacaaa agtgaagcca tcagatacga
    15301 ttgaagttga aggtattaaa atagaactag aagacaaaat ttacatttta ttccataaac
    15361 caactcaagt gataacaagt gtatctgatg atagaggacg tacagtggtt acagattact
    15421 ttaaagatat agaagcacgt atttatcctg ttggtcgttt agattatgat acttctggat
    15481 tgttattact tacaaatgat ggtgaattta ctaatttaat gacacatcca agatatcaaa
    15541 ttaagaagaa atatgtagca aaattaaaag gttacttaat gagagaagaa gtgaaagccc
    15601 tagaaaaagg tattgaatta gaagatggta tgacgcaacc ggctgaagtg aaagttaaga
    15661 aacaagataa agataaaaac acaacattgg ttgaaattac aattacagaa ggtcgtaatc
    15721 gccaagtcag aagaatgttc gaacattttg gtcatcaagt gacgaaactg tctcgtattg
    15781 aatatgggcc tttaaatgtt gtcggtttga atgctggtga aggacgtgta ttgacgcctc
    15841 atgaagtaaa agtgatgcgt catttagcag aacatgggaa ataattaaat aaaatatgta
    15901 tttatcacaa agtttgagaa ttttttcaca aaattttagg tttttaataa ctgtatgcgc
    15961 tttcctgtga tataatactt ttgtaaagaa aagcatgtgt gggaggtatg acctgtatgt
    16021 cgaacgaaat acttatcgta gatgatgagg atagaatcag aagattactt aaaatgtatt
    16081 tagaaagaga atcttttgaa atccatgaag caagtaatgg ccaagaggct tatgaacttg
    16141 caatggagaa taattatgct tgcatactac tagatttaat gttgcctgaa atggatggta
    16201 tccaggtggc aactaaattg cgtgaacata aacaaacacc gattattatg ttgactgcta
    16261 aaggtgaaga aacaaaccgt gttgaaggtt ttgaatctgg tgcagatgat tatatcgtca
    16321 aaccattttc accaagagaa gtagtcttaa gagttaaagc acttctaaga agaacgcaat
    16381 ctacaactgt agaacaaagc gaacctcacg cacgtgatgt gattgaattt aaacatttag
    16441 aaatagataa tgatgcacat cgcgtacttg ctgataatca agaagttaat ttgactccta
    16501 aagagtacga attattaata tatttagcta aaacaccaaa taaagtattt gaccgtgaac
    16561 aattattaaa agaagtttgg cattatgaat tctatggtga tttaagaaca gttgatactc
    16621 atgttaaacg acttagagaa aagttaaatc gtgtgtctag cgaagctgcg catatgattc
    16681 aaacagtctg gggcgttggg tataaatttg aggttaaatc taatgatgag ccggctaaat
    16741 agtgtcgtaa ttaaactgtg gttaactatt attttaatag tgacgacagt tttaatttta
    16801 ttaagtattg ctttaattac ctttatgcaa tactatttca cacaagaaac cgaaaatgcc
    16861 ataagagaag atgctagacg tataagttca ctggtcgaac aatcacataa taaagaagaa
    16921 gcaataaaat atagtcaaac attaattgaa aatcctggtg ggttgatgat tataaataat
    16981 aaacatcgtc aatcaacggc ttcactttct aatattaaaa agcaaatgtt gaatgaagta
    17041 gtcaacaacg accattttga cgatgtgttt gataaaggta aatctgttac tcgaaatgta
    17101 acgattaaag aaaagggttc atctcaaaca tatattttgt taggctatcc aacaaaagca
    17161 cagaagaata gtcatagcaa atatagtgga gtctttatat ataaagactt gaaatcaatc
    17221 gaagatacaa ataatgctat tacgattatc actataatta cggctgttat tttcttaaca
    17281 attacaacag tctttgcgtt tttcttatcg tcaagaatta caaaaccttt aagacgttta
    17341 agagaccaag ctacacgtgt atctgaaggg gattactctt ataaaccttc tgtcacaacg
    17401 aaagatgaaa ttggtcaatt atcgcaggca tttaatcaga tgagtacaga aatcgaagag
    17461 catgtcgacg cattatccac atctaaaaat attagagaca gcttaattaa ctctatggta
    17521 gaaggtgtcc taggtattaa tgagagtcga caaattatct tatctaataa gatggcgaat
    17581 gatattatgg acaatattga tgaagatgct aaagctttct tattaagaca aattgaagat
    17641 acttttaaat caaaacaaac ggaaatgcgt gatttagaaa tgaatgcacg attctttgtt
    17701 gtgaccacaa gctatatcga taagattgaa cagggaggta aaagtggtgt tgttgtgaca
    17761 gttcgtgata tgactaatga gcacaatcta gatcaaatga agaaagattt cattgctaat
    17821 gtatcacatg aattacgaac acctatatca ttactacaag gttatactga atcaattgta
    17881 gatggtattg ttacagaacc ggatgaaata aaagaatcgc tcgccgttgt ccttgatgaa
    17941 tcgaaacgtt taaatcgatt agttaatgaa ttgttaaatg tcgcacgcat ggatgctgaa
    18001 gggttatccg taaataaaga agttcagcct attgcagcgt tactagataa gatgaaaatt
    18061 aagtatcgcc aacaagctga tgatttaggt ctaaatatga cttttaatta ttgtaagaag
    18121 cgtgtttgga gttatgatat ggatcgcatg gaccaagtac taacgaactt aattgataat
    18181 gcatcacgtt atacgaaacc tggagatgaa attgcaatta cttgtgatga aaatgaaagc
    18241 gaagatattt tatacattaa agatacaggc acaggcattg caccagaaca tttacaacaa
    18301 gtatttgatc gtttttataa agttgatgca gcgagaacgc gaggtaaaca aggtaccggt
    18361 ttaggtttgt tcatttgtaa aatgattatc gaagagcatg gtggttccat tgatgttaaa
    18421 agcgaattag gaaaaggcac aacatttatt attaaactac caaaaccaga ataaaactga
    18481 atatagttat ttaagaacgc atgttattga ttagagactc taatttatag catgcgtttt
    18541 ttgattgatg tcgaaagttt tgtaagtgga ttaggattag ggtttttgcg aatatcaact
    18601 attaaatata ttactaattt atataaaaat ataaagtttg ataaagttat ttatttgatt
    18661 ataaaaatag ggtaaaatat agatatattg taataattaa attattcgag gtgtcatatg
    18721 aaaaaattca ttggatcagt tttagctacg acattaattt tagggggatg ttccatgatg
    18781 gaatatgaat caagtaaaga cacgaatac
//
LOCUS       DAHHSC010000032        18433 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.032, whole genome shotgun sequence.
ACCESSION   DAHHSC010000032 DAHHSC010000000
VERSION     DAHHSC010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 18433)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18433)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18433
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.032"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            197..1864
                     /locus_tag="NBI57_000868"
     CDS             197..1864
                     /locus_tag="NBI57_000868"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HCD1821832.1"
                     /translation="MTHLSDLDIANQSTLQPIKDIAASVGISEDALEPYGHYKAKIDI
                     NKITPRENKGKVVLVTAMSPTPAGEGKSTVTVGLADAFHELNKNVMVALREPALGPTF
                     GIKGGATGGGYAQVLPMEDINLHFNGDFHAITTANNALSAFIDNHIHQGNELGIDQRR
                     IEWKRVLDMNDRALRHVNVGLGGPTNGVPREDGFNITVASEIMAILCLSRSIKDLKDK
                     ISRITIGYTRDRKPVTVADLKVQGALAMILKDAIKPNLVQSIEGTPALVHGGPFANIA
                     HGCNSILATETARDLADIVVTEAGFGSDLGAEKFMDIKAREAGFDLAAVVVVATIRAL
                     KMHGGVAKDNLKEENVEAVKAGIVNLERHVNNIKKFGVEPVVAINAFIHDTDAEVEYV
                     KSWAKENNVRIALTEVWEKGGKGGVDLANEVLEVIDQPNSFKPLYELELPLEQKIEKI
                     VTEIYGGSKVTFSSKAQKQLKQFKENGWDNYPVCMAKTQYSFSDDQTLLGAPSGFEIT
                     IRELEAKTGAGFIVALTGAIMTMPGLPKKPAALNMDVTDDGHAIGLF"
     gene            complement(2109..2198)
                     /locus_tag="NBI57_000869"
     CDS             complement(2109..2198)
                     /locus_tag="NBI57_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1821833.1"
                     /translation="MLLTTPIRLYLIDNNYQYQLLVLIVAHPN"
     gene            2197..4872
                     /gene="harA"
                     /locus_tag="NBI57_000870"
     CDS             2197..4872
                     /gene="harA"
                     /locus_tag="NBI57_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haptoglobin-binding heme uptake protein HarA"
                     /protein_id="HCD1821834.1"
                     /translation="MNKHHPKLRSFYSIRKSTLGVASVIVSTLFLITSQHQAQAAENT
                     NTSDKISENQNNNATTTQQPKDTNQTQPATQPVITAKNYPAADESLKDAIKDPALENK
                     EHDIGPREQVNFQLLDKNNETQYYHFFSIKDPADVYYTKKKAEVELDINTASTWKKFE
                     VYENNQKLPVRLVSYSPVPEDHAYIRFPVSDGTQELKIVSSTQIDDGEETNYDYTKLV
                     FAKPIYNDPSLVKSDTNDAVVTNDQSSSDASNQTNTNTSNQNTSTTNNANNQPQATTN
                     MSQPAQPKSSANADQASSQPAHETNSNGNTNDKTNESSNQSDVNQQYPPADESLQDAI
                     KNPAIIDKEHTADNWRPIDFQMKNDKGERQFYHYASTVEPATVIFTKTGPVIELGLKT
                     ASTWKKFEVYEGDKKLPVELVSYDSDKDYAYIRFPVSNGTRDVKIVSSIEYGENIHED
                     YDYTLMVFAQPITNNPDDYVDEETYNLQKLLAPYHKAKTLERQVYELEKLQEKLPEKY
                     KAEYKKKLDQTRVELADQVKSAVTEFENVTPTNDQLTDLQEAHFVVFESEENSESVMD
                     GFVEHPFYTATLNGQKYVVMKTKDDSYWKDLIVEGKRVTTVSKDPKNNSRTLIFPYIP
                     DKAVYNAIVKVVVANIGYEGQYHVRIINQDINTKDDDTSQNNTSEPLNVQTGQEGKVA
                     DTDVAENSSTATNPKDASDKADVIEPESDVVKDADNNIDKDVQHDVDHLSDMSDNNHF
                     DKYDLKEMDTQIAKDTDRNVDNSVGMSSNVDTDKDSNKNKDKVIQLAHIADKNNHTGK
                     AAKLDVVKQNYNNTDKVTDKKTTEHLPSDIHKTVDKTVKTKEKAGTPSKENKLSQSKM
                     LPKTGETTSSQSWWGLYALLGMLALFIPKFRKESK"
     gene            5003..5095
                     /locus_tag="NBI57_000871"
     CDS             5003..5095
                     /locus_tag="NBI57_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1821835.1"
                     /translation="MKIILLLFLIFGCIVVVTLKSEHQLTLFSI"
     gene            complement(5075..5980)
                     /locus_tag="NBI57_000872"
     CDS             complement(5075..5980)
                     /locus_tag="NBI57_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HCD1821836.1"
                     /translation="MTNQDNNHQLNHRIYHFEKIYKAIKHVIVFIFMIFIAIVAIAVI
                     AMSLYFHHLTKTSDSLSDDALIKKVRQIPGDELLDHNNKNLLYEYNHSQNSLIIGPKT
                     SSPNVIKALTSSEDTLFYKHDGILPKAILRAMIQDIFNTDQSSGGSTITQQLVKNQVL
                     TNEKTYSRKANELRLAIRLEHLLSKDEIIYTYLNIVPFGRDYNGANISGIASASYSLF
                     GIPPKDLSIAQSAYLIGLLQSPYGYTPYEKDGTLKSDKDLKYSIQRQHYVLKRMLIED
                     QITEKEYNDALKYDIKSHLLNRKKR"
     gene            6362..7624
                     /locus_tag="NBI57_000873"
     CDS             6362..7624
                     /locus_tag="NBI57_000873"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HCD1821837.1"
                     /translation="MTNVLIEDLKWRGLIYQQTDEQGIEDLLNKEQVTLYCGADPTAD
                     SLHIGHLLPFLTLRRFQEHGHRPIVLIGGGTGMIGDPSGKSEERVLQTEEQVDKNIEG
                     ISKQMHNIFEFGTDHGAVLVNNRDWLGQISLISFLRDYGKHVGVNYMLGKDSIQSRLE
                     HGISYTEFTYTILQAIDFGHLNRELNCKIQVGGSDQWGNITSGIELMRRMYGQTDAYG
                     LTIPLVTKSDGKKFGKSESGAVWLDAEKTSPYEFYQFWINQSDEDVIKFLKYFTFLGK
                     EEIDRLEQSKNEAPHLREAQKTLAEEVTKFIHGEDALNDAIRISQALFSGDLKSLSAK
                     ELKDGFKDVPQVTLSNDTTNIVEVLIETGISPSKRQAREDVNNGAIYINGERQQDVNY
                     ALAPEDKIDGEFTIIRRGKKKYFMVNYQ"
     gene            complement(7718..8992)
                     /locus_tag="NBI57_000874"
     CDS             complement(7718..8992)
                     /locus_tag="NBI57_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HCD1821838.1"
                     /translation="MSDFNHTDHSTTNHSQTPRYRRPKFPWFKTVIVALIAGIIGALL
                     VLGIGKVLNSTILNKDGSTVQTTNNKGGNQLDGQSKKFGTVHEMIKSVSPTIVGVINM
                     QKASSVDDLLKGKSSKPSEAGVGSGVIYQINNNSAYIVTNNHVIDGANEIRVQLHNKK
                     QVKAKLVGKDAVTDIAVLKIENTKGIKAIQFANSSKVQTGDSVFAMGNPLGLQFANSV
                     TSGIISASERTIDAETTGGNTKVSVLQTDAAINPGNSGGALVDINGNLVGINSMKIAA
                     TQVEGIGFAIPSNEVKVTIEQLVKHGKIDRPSIGIGLINLKDIPEEEREQLHTDREDG
                     IYVAKADSDIDLKKGDIITEIDGKKIKDDVDLRSYLYENKKPGESVTVTVIRDGKTKE
                     VKVKLKQQKEQPKRQSRSERQSPGQGDRDFFR"
     gene            9164..9781
                     /locus_tag="NBI57_000875"
     CDS             9164..9781
                     /locus_tag="NBI57_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HCD1821839.1"
                     /translation="MYSVISKILNFILVKMSKSLYVIGKDNIPKDSKYVVTCTHESYN
                     EVIMLGMALYPNQIHYMAKKELFKNKWIGKFLTSLNAFPVDRENPGPSTLKRPINLLK
                     DNKTVGIFPTGSRTSQEGAPLKRGASTIAMLSKSPILPVAYVGPTKIHGLLTGQAYIN
                     IGKPIDINDLPKDLKRNERIDYITKEIETRTAKLQQELHEIVKSL"
     gene            9909..11375
                     /locus_tag="NBI57_000876"
     CDS             9909..11375
                     /locus_tag="NBI57_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIBC"
                     /protein_id="HCD1821840.1"
                     /translation="MYKFFQNLGRSLMLPVAILPAAAIIAGIGNTLNALHATPKIAMF
                     FTTVGTTILEQLGILFAIGVAIGMAKKNDGAVALAATLGYFLVTVVLSPMKLAPLLGM
                     KASEINSAFEKMNNGNVFVGIVIGLIAAYAYNKFSETELPLALSFFSGKRLVPIMTAF
                     YCTFLVVILLFLWPLLYSWIVKFGESIVGLGSFGAFIYGVANRLLIPTGLHHALNSVF
                     WFDTIGINDIGKFQSGKDAIKGITGRYQAGFFPIMMFGIPAAALAMYHTAKTTQKKQV
                     YGWFLASSVAAFFVGVTEPIEFAFMFVAPILYVVHALLTGLSLFIAATFHWTAGFSFS
                     AGLIDYVLSLINPVSNHPLMLLVQGVVFFILYYVIFRVVIQVFNLNTIGRGENELVDP
                     TVVKDNIAPGENDIKQSKYHQHAIQILEGLGGQENIVNLTNCATRLRLELKDTSIIDQ
                     QKIKNAGAVGVTVNGKHSTQVIVGTHVQQVADEIEKHL"
     gene            11482..12612
                     /locus_tag="NBI57_000877"
     CDS             11482..12612
                     /locus_tag="NBI57_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HCD1821841.1"
                     /translation="MKKILFDVDGIFLSEERCFDVSALTVYELLMDKCYLGLHSHIDW
                     ETLTDNDIQDIRNRIFQKDKILNKLKSLGLNSNWDMLFIVFSIHLIDILKKLSHDEIE
                     AFMYQDEPVELKLQNISTNLADCFNLNEQLPLQFLDNVKVGKNNIYAALEEFATTELH
                     VSDATLFSLKGALWTLAQEVYQEWYLGSKLYEDVEKKIARTTFKTGYIYQEIILRPVD
                     EVKVLLNDLKGAGFELGIATGRPYTETVVPFENLGLLPYFEADFIATASDVLEAENMY
                     PQARPLGKPNPFSYIAALYGNNRDKYESYINKQDNIVNKDDVFIVGDSLADLLSAQKI
                     GATFIGTLTGLKGKDAAGELEAHHADYVINHLGELRGVLDNL"
     gene            complement(12573..12668)
                     /locus_tag="NBI57_000878"
     CDS             complement(12573..12668)
                     /locus_tag="NBI57_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1821842.1"
                     /translation="MKPLVQSFTSSYAVKQQSNYKLSSTPLSSPK"
     gene            complement(12762..14366)
                     /locus_tag="NBI57_000879"
     CDS             complement(12762..14366)
                     /locus_tag="NBI57_000879"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HCD1821843.1"
                     /translation="MKQFNVLVADPISKDGIKALLDHEQFNVDIQTGLSEEALIKIIP
                     SYHALIVRSQTTVTENIINAADSLKVIARAGVGVDNININAATLKGILVINAPDGNTI
                     SATEHSLAMLLSMARNIPQAHQSLTNKEWNRNAFKGTELYHKTLGVIGAGRIGLGVAK
                     RAQSFGMKILAFDPYLTDEKAKSLSITKATVDEIAQHSDFVTLHTPLTPKTKGLINAD
                     FFAKAKPSLQIINVARGGIIDEKALIKALDEGQISRAAIDVFEHEPATDSPLVAHDKI
                     IVTPHLGASTVEAQEKVAISVSNEIIEILIDGTVTHAVNAPKMDLSNIDDTVKSFINL
                     SQTVGELAIQLMYNAPSSIKITYGGDLASIDSSLLTRTIITHILKDDLGPEVNIINAL
                     MLLNQQQVTLNIENNKAETGFSNYLEVELSNDSDSVKVGASVFTSFGPRIVRINNFSV
                     DLKPNQYQIVSYHNDTPGMVGKTGALLGKYNINIASMTLGRTEAGGDALMILSVDQPV
                     SNNIIDELKQVGEYNQIFTTELTVQS"
     gene            complement(14353..15510)
                     /locus_tag="NBI57_000880"
     CDS             complement(14353..15510)
                     /locus_tag="NBI57_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HCD1821844.1"
                     /translation="MYYHQPLLLTPGPTPVPDAIMREIQAPMVGHRSKDFEDIAQQAF
                     QGLKPIFGSQNDVLILTSSGTSVLEASMLNIVNPEDHFVVIVSGAFGNRFKQIAQTYY
                     KNVHIYDVTWGEAVDVKDFINYLSTLDVEVKAVFSQYCETSTTVLHPIHELGNAINQF
                     NSNIYFVVDGVSCIGAVDVDINKDKIDVLVSGSQKAIMLPPGLAFVAYSHRAKERFKE
                     VTTPKFYLDLNKYISSQADNSTPFTPNVSLFRGVNAYVETVKAEGFNHVIARHYAIRN
                     ALRSALKALDLTLLVNDKDASPTVTAFKPNTNEVKIIKDELKNRFKITIAGGQGHLKG
                     QILRIGHMGKISPFDILSVVSALEIILTEHRKVNYIGKGISKYMEVIHEAI"
     gene            complement(15625..16071)
                     /locus_tag="NBI57_000881"
     CDS             complement(15625..16071)
                     /locus_tag="NBI57_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HCD1821845.1"
                     /translation="MHQHDFKVQTSWQGGRNNVGNVQGDILSENISIPASLGGVGIGT
                     NPDEMLVSAASSCYIISLAATLERAKFTDISIEQQSIGTACLNNGKFSMSKIVHHPHI
                     EIPSDQIAQLEKRLPKLITIADNNCMISNAVRNNVDIKIYPIIQAK"
     gene            16151..16894
                     /locus_tag="NBI57_000882"
     CDS             16151..16894
                     /locus_tag="NBI57_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HCD1821846.1"
                     /translation="MTLNKLKDELQIVSHRGLPSDFPENTMVGYREVMGLNVAMLEID
                     VHLTKDQHFVVIHDETIDRTSDGKGRIADYTLSQLKSFDFGSYKDVAFKGERIPTLDE
                     VLSLCLKYDKKLLIELKSPNLYPEIECKLLAFLEEKKVDATQVVIQSFDIECIEKLNT
                     LGSIYELGVLCSKRNYWYKKPNFSRIAQIASYVNPNYALVARKFVDKAHHHQLQVMPY
                     TVNKLKTGEKLRQMGVDGLITDNPKLFIK"
     gene            complement(17110..17712)
                     /gene="rpsD"
                     /locus_tag="NBI57_000883"
     CDS             complement(17110..17712)
                     /gene="rpsD"
                     /locus_tag="NBI57_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019165370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HCD1821847.1"
                     /translation="MARFRGSNWKKSRRLGISLSGTGKELEKRPYAPGQHGPNQRKKL
                     SEYGLQLREKQKLRYLYGMTERQFRNTFDIAGKKFGVHGENFMILLASRLDAVVYSLG
                     LARTRRQARQLVNHGHILVDGKRVDIPSYSVKPGQTISVREKSQKLNIIVESVEINNF
                     VPEYLNFDADSLTGTFVRLPERSELPAEINEQLIVEYYSR"
     gene            complement(17956..18420)
                     /locus_tag="NBI57_000884"
     CDS             complement(17956..18420)
                     /locus_tag="NBI57_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HCD1821848.1"
                     /translation="MTTINPTNYTLLKKQAASLIEDEHHMIAILSNMSALLNDNLNQI
                     NWVGFYLLEQNELILGPFQGHPACVHIPIGKGVCGTAVSERRTQVVADVHQFEGHIAC
                     DANSKSEIVVPIFKDDKIIGVLDIDAPITDRFDDNDKEHLEAIVKIIEKQLA"
BASE COUNT         6288 a         2790 c         3299 g         6056 t
ORIGIN      
        1 tatcaatagt atcaagcgct aaaagtttta taatacaaaa ctttaataag tgaatttatt
       61 gcaaaaatga aagcgctaac ccgatttagt cgacaagttt ttaacagttc gttattatat
      121 gaatgtaagt aaaaatttct tagctacaac ttacatatta taaatgcata aattaaacaa
      181 aaaggggcga aaaaagttga ctcatttatc agatttagat attgcgaatc aatcaacact
      241 acaaccaatt aaggatattg ctgcatcagt aggtatttca gaggatgcat tagaacctta
      301 tggtcattac aaagctaaaa tcgacattaa taaaattacg ccaagagaaa acaaagggaa
      361 agttgtttta gtaactgcga tgagcccaac accagctggt gaaggtaaat caacggttac
      421 agttggttta gctgatgcat tccatgagtt aaataaaaac gttatggttg cattaagaga
      481 gcctgcttta ggaccaacat ttggtatcaa aggtggtgcg actggtggtg gttatgcgca
      541 agtcttacct atggaagata tcaacttaca tttcaacgga gatttccatg cgattacaac
      601 tgcaaataat gcattgtctg cgtttataga taatcatatt caccaaggta atgaattagg
      661 aatcgatcaa agacgtattg aatggaaacg tgtattagat atgaatgatc gtgcacttag
      721 acatgtaaac gttgggttag gtggacctac aaatggtgta ccacgtgaag atggctttaa
      781 tattacagta gcgtctgaaa ttatggcgat tttatgttta agtagaagta ttaaagactt
      841 aaaagataaa attagtcgta ttactattgg ttacactaga gatcgcaagc cagttacagt
      901 tgcagattta aaagtgcaag gtgcacttgc aatgatttta aaagatgcaa ttaaaccaaa
      961 cttagtacaa tcaattgaag ggacacctgc attagttcat ggtggaccat ttgcgaatat
     1021 cgcacacggt tgtaactcaa ttttagcaac tgaaacagca cgtgatttag ctgatatcgt
     1081 tgtaacggaa gctggatttg gttcagactt aggcgctgaa aaattcatgg acattaaagc
     1141 gcgtgaagca ggatttgatc tggcagctgt cgttgttgtt gcgacaattc gtgcgttaaa
     1201 aatgcatggt ggtgtagcga aagataattt aaaagaagaa aatgtagaag cagtaaaagc
     1261 aggaattgtt aatttagagc gtcatgttaa taatattaaa aaattcggtg tagaaccggt
     1321 tgttgcaatt aatgcattta tacatgatac cgatgcagaa gtagaatatg taaaatcttg
     1381 ggctaaagaa aataacgtac gaattgcctt aactgaagtt tgggaaaaag gtggtaaagg
     1441 tggcgttgac ttagcaaatg aagtattaga agtcattgat caacctaatt catttaaacc
     1501 tttatatgaa ttagaattac cattagagca aaagattgaa aagattgtga ctgaaatcta
     1561 tggcggttca aaagtaacgt ttagcagtaa agcgcaaaaa caattaaaac aatttaaaga
     1621 aaatggttgg gataattacc cagtatgtat ggcgaaaaca caatattcat tctcagatga
     1681 tcaaacgttg ttaggtgcac catcaggatt tgaaattaca attcgtgaat tagaagcgaa
     1741 aacaggtgca ggatttatcg tagcgttgac aggtgcaatc atgactatgc ctggtttacc
     1801 taaaaaacca gcagcattaa acatggatgt tactgatgat ggtcatgcaa ttgggttatt
     1861 ctaataaatc atgtcaattg tttaataaag ataagtaaat agtttaatag accggactgt
     1921 tggagatgca ttatttcagc agttcggttt tttgctgtgc taaaaataga ttccatttgg
     1981 cgaatctaac gacaatgttt gaaggtggtt aattaatgta tatgaagata aaaagtgggt
     2041 ttgaagaata ggaaagcgat gcaatgaata ttcatattaa aaaaatttaa taaaataggt
     2101 tgcaatattt aattgggatg cgctacaatt aacactaata attgatattg ataattatta
     2161 tcaattaaat ataatcttat aggagttgtt aacaacatga acaaacatca cccaaaatta
     2221 aggtctttct attctattag aaaatcaact ctaggcgttg catcggtcat tgtcagtaca
     2281 ctatttttaa ttacttctca acatcaagcg caagcagcag aaaatacaaa tacttcagat
     2341 aaaatctcgg aaaatcaaaa taataatgca actacaactc agcaacctaa ggatacaaat
     2401 caaacacaac ctgctacgca accagtaatc actgcgaaaa actatcctgc agcggatgaa
     2461 tcacttaaag atgcaattaa agatcctgca ttagaaaata aagaacatga tataggtcca
     2521 agagaacaag tcaatttcca gttattagat aaaaacaatg aaacgcagta ctatcacttt
     2581 ttcagcatca aagatccagc agatgtgtat tacactaaaa agaaagcaga agttgaatta
     2641 gacatcaata ctgcttcaac atggaaaaag tttgaagtct atgaaaacaa tcaaaaatta
     2701 ccagtgagac ttgtatcata tagtcctgta ccagaagacc atgcctatat tcgattccca
     2761 gtttcagatg gcacacaaga attgaaaatt gtttcttcga ctcaaattga tgatggagaa
     2821 gaaacaaatt atgattatac taaattagta tttgctaaac ctatttataa cgatccttca
     2881 cttgtgaaat cagatacaaa tgatgcagta gtaacgaatg atcaatcaag ttcagacgca
     2941 agtaatcaaa caaacacgaa tacatctaat caaaatacat caacgaccaa caatgctaat
     3001 aatcaaccgc aggcaacgac aaatatgagt caacctgcac aaccaaaatc gtcagcgaat
     3061 gcagatcaag cgtcaagcca accagctcat gaaacaaatt ctaatggtaa tactaacgat
     3121 aaaacgaatg agtcaagtaa tcagtcggat gttaatcaac agtatccacc agcagatgaa
     3181 tcactacaag atgcaattaa aaacccggct atcatcgata aagaacatac agctgataat
     3241 tggcgaccaa ttgattttca aatgaaaaat gataaaggtg aaagacagtt ctatcattat
     3301 gctagtactg ttgaaccagc aactgtcatt tttacaaaaa caggtccagt aattgagtta
     3361 ggtttaaaga cagcttcaac atggaagaaa tttgaagttt atgaaggtga caaaaagtta
     3421 ccagtcgaat tagtatcata tgattctgat aaagattatg cctatattcg tttcccagta
     3481 tctaatggta cgagagacgt taaaattgtg tcatctattg aatatggtga gaacatccat
     3541 gaagactatg attatacgct aatggtcttt gcacagccta ttactaataa cccagacgac
     3601 tatgtggatg aggaaacata caatttacaa aaattattag ctccgtatca caaagctaaa
     3661 acgttagaaa gacaagttta tgaattagaa aaattacaag agaaattgcc agaaaaatat
     3721 aaggcggaat ataaaaagaa attagatcaa actagagtag agttagctga tcaggttaaa
     3781 tcagcagtga cggaatttga aaatgttaca cctacaaatg atcaattaac agatttacaa
     3841 gaagcgcatt ttgttgtttt tgaaagtgaa gaaaatagtg agtcagttat ggacggcttt
     3901 gttgaacatc cattctatac agcaacttta aatggtcaaa aatatgtagt gatgaaaaca
     3961 aaggatgaca gttactggaa agatttaatt gtagaaggta aacgtgtcac tactgtttct
     4021 aaagatccta aaaataattc tagaacgttg attttcccat atatacctga caaagcagtt
     4081 tacaatgcga ttgttaaagt cgttgtggca aacattggtt atgaaggtca atatcatgtc
     4141 agaattataa atcaggatat caatacaaaa gatgatgata catcacaaaa taacacgagt
     4201 gaaccgctaa atgtacaaac aggacaagaa ggtaaggttg ctgatacaga tgtagctgaa
     4261 aatagcagca ctgcaacaaa tcctaaagat gcgtctgata aagcagatgt gatagaacca
     4321 gagtctgacg tggttaaaga tgctgataat aatattgata aagatgtgca acatgatgtt
     4381 gatcatttat ccgatatgtc ggataataat cacttcgata agtatgattt aaaagaaatg
     4441 gatactcaaa ttgccaaaga tactgataga aatgtggata atagcgttgg tatgtcatcg
     4501 aatgtcgata ctgataaaga ctctaataaa aataaagaca aagtcataca gcttgctcat
     4561 attgccgata aaaataatca tactggaaaa gcagcaaagc ttgacgtagt gaaacaaaat
     4621 tataataata cagacaaagt tactgacaaa aaaacaactg aacatctgcc gagtgatatt
     4681 cataaaactg tagataaaac agtgaaaaca aaagaaaaag ccggcacacc atcgaaagaa
     4741 aacaaactta gtcaatctaa aatgctacca aaaactggag aaacaacttc aagccaatca
     4801 tggtggggct tatatgcgtt attaggtatg ttagctttat tcattcctaa attcagaaaa
     4861 gaatctaaat aattaactaa atatagcata tgtatggtta actttgtaaa caatgtgaaa
     4921 gcaattaatt tataaactat tgattggttt aatggctttc ctttagagta aataaaaaga
     4981 acagcagtga gaaattttct aattgaaaat aatcttactg ctgtttttaa tatttggatg
     5041 cattgttgtg gttactttaa aaagtgagca tcaattaacg cttttttcga tttaacaaat
     5101 gtgatttaat atcatatttt aatgcgtcgt tgtattcttt ttcagtgatt tgatcttcga
     5161 ttaacatacg ctttaataca taatgttgtc tttgaatact atatttcaaa tctttatccg
     5221 attttaacgt tccatctttt tcgtagggtg tatagccata agggctttgc aacaaaccga
     5281 taaggtatgc agattgtgca attgataaat cttttggtgg aataccaaac aaactatatg
     5341 aagcggatgc aattccggaa atattagcgc cattataatc tctaccgaag ggaactatat
     5401 ttaaatatgt atatataatt tcatcttttg agagtaggtg ttctaatcta attgctaggc
     5461 gaagttcatt tgcttttcta ctatatgttt tttcgttggt aagaacttga tttttaacaa
     5521 gttgttgtgt aattgtgcta ccacctgaac tttgatcagt attaaaaata tcttgtatca
     5581 ttgctcttaa aatcgccttt ggtaagatgc catcatgttt ataaaataaa gtgtcttcag
     5641 atgatgttaa tgctttaatg acatttggac ttgatgtttt agggcctata atgagtgagt
     5701 tttgagaatg gttatactca tataataaat ttttgttatt atgatctaat aattcatcgc
     5761 caggtatttg tcgaactttt tttattaaag catcatctga taatgagtcg gacgttttag
     5821 ttaaatgatg aaaatataaa gacatcgcaa tcacagcgat agcaacgatg gcaatgaaaa
     5881 tcataaatat aaaaacaatg acatgtttga tagctttata tatcttttca aaatgatata
     5941 tacgatgatt caattgatga ttgttgtctt gattcgtcat ctttaacctc ctaaattacg
     6001 tatagtatac catatttaag caatttgact tttattttaa cgttaattat aattagggaa
     6061 aatgcataaa gaataggata aaaggacaag taatattatt tgaaatcatc agagagctag
     6121 tggtcgctgt gaactagtgt ttttaataat atgaatacac ctttataact aacatgtcct
     6181 acgtttatta atgaacgtat cattaataga gtgaacatta tcttatgatt atgtttaagt
     6241 ttggtggtaa cgtgcaatcg cgcccttaca tatttgttgt aagggttttt tttacgttca
     6301 aaaaacttta aagtatttta acaagtattc aataaataat tataaatagg aggaaataat
     6361 aatgacgaat gtattaattg aagatttaaa atggagaggt cttatttatc aacaaactga
     6421 tgaacaaggt attgaagatt tattaaataa agaacaagtg acgttatact gcggtgccga
     6481 tccaacggca gatagtttac atattggtca cttactacca ttcttaacat taagacgttt
     6541 tcaagaacat ggacatcgtc ctatcgtttt aattggcggt ggtacaggta tgattggtga
     6601 tccatcaggt aaatcagaag aacgtgtgct acaaacagaa gaacaagtag ataaaaatat
     6661 cgaaggtatt agtaagcaaa tgcacaatat ttttgaattt ggaacagacc atggtgcagt
     6721 gcttgttaat aatagagact ggttaggaca aatctcatta attagctttt tacgtgacta
     6781 tggtaaacac gtcggcgtta attacatgtt aggtaaagat tcaatccaaa gtcgtttaga
     6841 acatggtatt tcatatacag aattcacata cacgatttta caagctattg atttcggtca
     6901 tttgaataga gaattgaatt gtaagattca agtaggtgga tcagatcaat ggggtaatat
     6961 cacaagtggt attgaattaa tgcgtcgtat gtatggtcaa acagacgcat acggtttaac
     7021 tattccgctt gtaactaaat cagatggtaa gaaatttggt aagtctgagt caggtgctgt
     7081 ttggttagat gctgaaaaaa caagtcctta tgaattttat caattctgga ttaatcaatc
     7141 agacgaagat gtaattaaat tcttaaaata ctttactttc ttaggaaaag aagaaattga
     7201 tcgcttagaa caatctaaaa atgaagcacc gcatttacgt gaagctcaaa aaacattagc
     7261 tgaagaagta actaaattta ttcatggtga agatgcatta aatgatgcaa tccgtatttc
     7321 acaagcatta tttagtggtg atttaaaatc attatcagcg aaagaattaa aagatggatt
     7381 taaagatgtg cctcaagtga cattatcaaa tgacacaaca aatatcgttg aagtccttat
     7441 tgaaacaggc atttctcctt ctaaacgaca agcacgtgaa gatgttaaca atggtgcgat
     7501 ttatattaat ggtgagagac aacaagatgt taattatgct ttagcaccag aagataaaat
     7561 tgatggcgaa tttacgatta ttcgtcgcgg taagaaaaaa tacttcatgg ttaactatca
     7621 ataaatataa ttgcatagct aaataaatta gagcctactc atattcattc ctaagaatgt
     7681 aatgagtagg ctcttaatgt acttttctgt ctgtaaatta tctaaagaaa tctctatcgc
     7741 cttggccagg tgattgacgt tctgatcggc tttgacgttt tggttgttct ttttgttgtt
     7801 ttaatttcac tttaacttct tttgttttac catcacggat aacggtaaca gtgactgatt
     7861 caccaggttt tttattttca tataaatagc ttcttaaatc aacatcatct ttaattttct
     7921 tgccatcaat ttctgtaata atatcacctt ttttaagatc aatatcacta tcagctttgg
     7981 cgacataaat accgtcttct ctatcagtat gaagttgctc gcgctcttct tcaggaatat
     8041 ctttcaaatt aattaaacca ataccaatcg aagggcggtc aattttacca tgttttacaa
     8101 gttgttcaat tgttacttta acttcattac ttggaatagc aaacccgata ccttcaactt
     8161 gtgtcgcagc aattttcatt gagttaatac caactaaatt accattaata tctactaatg
     8221 cgccacctga gttacctggg ttaatagcag catctgtttg aagaacgcta acttttgtat
     8281 tgccaccagt tgtctcagcg tcaatcgtac gttcgcttgc tgaaatgata ccagatgtta
     8341 cagagttagc aaattgtaat cctaatgggt tacccattgc gaatacgcta tcgccagttt
     8401 gtacttttga agagttggca aattgaatcg ctttaatacc ttttgtattt tcaattttaa
     8461 gtacagcaat atcagttact gcatctttac caactaattt cgctttaact tgttttttat
     8521 tatgtaattg gactctaatt tcatttgcgc catcaataac atgattgttt gtaacgatat
     8581 aagctgaatt gttgtttatt tgatagataa cacctgaacc tactccagct tcagatggtt
     8641 tagatgattt gccttttaat aagtcgtcta cacttgatgc tttttgcatg ttaataactc
     8701 caacaattgt aggggagaca gattttatca tttcatgaac ggtaccgaat ttcttgcttt
     8761 gaccgtctaa ttgattgcca cctttattat ttgttgtctg aacagttgaa ccatctttat
     8821 ttaaaattgt actatttaat actttgccta taccaagtac tagaagtgca ccaataattc
     8881 cagcaatcaa tgcaacgatg actgttttaa accatggaaa tttaggtctt ctgtatctag
     8941 gtgtttggct atggtttgtt gtagaatgat ctgtatgatt aaaatctgac atacttaacc
     9001 tccattatat gatttatata tgctttaatt atgtcttttt tttataatta ttgctagtaa
     9061 tacactatac aactttagat gtattttcag actatcttgt tctagtaaaa atatagtgat
     9121 aaaatctagt tgttataaac aaataagtta agtaggtggc acaatgtatt cagtgattag
     9181 taagattttg aatttcattt tagttaaaat gtcaaaatca ttatacgtaa tcggtaaaga
     9241 taatattcct aaggatagta aatatgtcgt aacttgtacg catgaaagtt ataacgaagt
     9301 tattatgcta ggtatggcat tatatcctaa tcaaattcat tatatggcta aaaaagaatt
     9361 attcaaaaat aaatggattg gtaaattttt aacgtcttta aatgcatttc ctgtagatcg
     9421 tgaaaatcca ggacctagta ctttaaaacg cccaatcaat ttgttgaaag ataataagac
     9481 agttggtatc ttcccaacag gttctcgcac atcgcaagaa ggtgcaccat tgaaacgtgg
     9541 tgcttcaaca attgcaatgt taagtaagtc accaatatta cctgttgctt atgttggtcc
     9601 tactaagatt catggtcttt taacaggaca agcatacata aatattggta aaccaatcga
     9661 tattaacgat ttacctaaag atttaaaacg aaacgagcgt atcgattaca ttacaaaaga
     9721 gattgaaaca cgaacagcaa agttacaaca agaattgcat gaaattgtaa aaagtttata
     9781 aaataacttg aggtagaaat atagctggtt agtatgccta ggtgactata acggagtatc
     9841 gaagtagtta ttctagttac attagtaatc tttatatttc taccttattt taagaaggat
     9901 gacattcaat gtataaattt ttccaaaatt tagggcgttc gttaatgtta ccagttgcca
     9961 ttttaccggc tgcagcaatt attgccggaa ttggtaatac gttaaatgca cttcatgcga
    10021 cgccgaaaat cgcaatgttc tttactacgg taggtactac gattttagag caattaggta
    10081 ttttatttgc aattggtgta gctataggaa tggcaaagaa aaatgatggt gcagtcgcgc
    10141 ttgcagctac gttaggatat tttttagtta cagttgtatt atcaccaatg aaattagcgc
    10201 ctttattagg aatgaaagct tcggaaataa attcagcttt tgaaaagatg aataatggca
    10261 atgtctttgt tggtattgta ataggtttaa tagcagccta tgcatacaat aagtttagtg
    10321 aaacagaatt accattagca ttatcatttt ttagtggtaa acgtctagtt ccaattatga
    10381 ctgcatttta ctgtacattt ttagttgtca tattgttatt cttatggcca ctactttatt
    10441 catggattgt aaaatttggt gaatctatag tcggtttagg atcgtttgga gctttcatat
    10501 atggtgttgc caatagatta ttaattccta caggattaca tcatgcactg aatagtgtgt
    10561 tctggtttga tacgattggt attaatgata ttggtaaatt ccaaagtggc aaagatgcta
    10621 taaaaggtat tacaggacgc tatcaagcgg gattcttccc aatcatgatg ttcggtattc
    10681 cagcggctgc gttagcaatg tatcatacag ctaaaacgac tcagaaaaaa caagtttacg
    10741 gttggttcct agcgagttca gttgcagcat ttttcgttgg tgttacagag ccgattgagt
    10801 ttgcatttat gtttgttgcg ccaatcttat atgttgttca tgcgttatta acaggattat
    10861 ctttatttat cgcagctaca ttccattgga cagctggatt ttcatttagt gcaggtttga
    10921 tagattatgt gttatcacta attaatccag tatcaaatca tccattgatg ttgcttgttc
    10981 aaggtgttgt atttttcatc ttatactatg tgattttccg tgtagtaatc caagtattta
    11041 acttgaatac gattggtaga ggtgaaaatg aattagttga tccaacagtt gtaaaagata
    11101 atattgctcc tggtgaaaat gatattaaac aaagtaaata tcatcaacat gctatacaaa
    11161 tattagaagg tttaggcggt caagagaata ttgttaattt aaccaattgt gcaacaaggt
    11221 tgcgtctaga gttaaaagac acatctatta ttgatcaaca gaagattaaa aacgccggtg
    11281 cagttggtgt tactgttaat ggcaaacatt caactcaagt tattgtcggt acacacgtac
    11341 agcaggttgc tgatgaaatt gaaaaacatt tataaaattt aaacagctaa agcgcaaatc
    11401 taacttcaat agggtttgcg ctttttttag tcaaatagta gaatgattta tatctgaatt
    11461 ttcagaaatg gagtggggat tgtgaagaaa attttatttg atgttgatgg cattttttta
    11521 agtgaagaac gttgtttcga tgtctctgca ttaacggtat atgaattatt aatggataag
    11581 tgttatttag gtcttcattc acatatagat tgggaaactt tgactgataa cgatatacaa
    11641 gacattagga atcgaatttt tcaaaaagat aagattttaa acaaattaaa atcgctaggg
    11701 ctgaattcaa attgggatat gttatttatc gtttttagta ttcatttaat tgatattttg
    11761 aaaaagttat cacatgatga aattgaggca ttcatgtatc aagatgagcc tgtcgaattg
    11821 aagttgcaaa atataagcac aaacttagca gactgtttta atttaaatga acaactacct
    11881 ttacaatttt tggataatgt aaaagttggt aaaaataata tttatgctgc tttggaagag
    11941 tttgcaacaa cagagttaca tgtttcggat gctactttat ttagtttgaa gggtgcatta
    12001 tggacgttag cgcaagaagt ttatcaagaa tggtatttag gatcgaagtt gtatgaagat
    12061 gttgaaaaga aaatagcacg aactactttt aagacaggtt atatttatca agaaattatt
    12121 ttgagaccag tagatgaagt taaggtactt ctgaatgatt taaaaggtgc tggtttcgaa
    12181 ttaggtattg caacaggtcg tccttatact gagactgttg tgccatttga aaatttagga
    12241 ttgttaccat attttgaagc tgattttatt gcaacagcaa gtgatgtttt agaagcagag
    12301 aatatgtatc cgcaagcacg accattagga aagccgaatc cttttagtta tatcgcagct
    12361 ttatatggta ataatcgcga taaatatgaa tcttatatca ataagcaaga taacattgta
    12421 aataaagatg acgtatttat agtaggcgat tcgttagctg acttattaag tgctcaaaaa
    12481 ataggtgcaa cgtttattgg aacattaaca ggtttaaaag gtaaggatgc tgcaggtgag
    12541 ttagaagcgc atcatgccga ctatgttatt aatcatttag gtgaacttag aggtgtacta
    12601 gataatttgt aatttgattg ttgtttgaca gcataacttg aagtgaatga ttgaaccaaa
    12661 ggtttcatat tgagttacaa tgaaattaat aatgaaaaaa tgccaagaag caatggaagt
    12721 aatccaatgt cttcttggca ttttgaattt acataaattg tttatgactg taccgtcaat
    12781 tcagttgtga aaatttgatt gtattcacca acttgtttaa gttcatcaat tatattgttt
    12841 gaaacaggtt gatcaacgga taaaatcatt agcgcatctc cgcccgcttc agttctacct
    12901 aaagtcatag atgcaatgtt gatattgtat ttacctaaca atgcgccagt ttttcctacc
    12961 atacctggag tatcattatg atatgacaca atttgatatt gatttggctt taagtctaca
    13021 gaaaaattat taattctaac aattcttgga ccgaaacttg taaagacaga agcgccaact
    13081 ttaacggaat cgctatcgtt tgatagttct acctctaagt agttactaaa acctgtctct
    13141 gctttattat tttcaatatt taatgtcact tgttgttgat ttaacaacat tagagcattg
    13201 ataatattga cttcaggacc aagatcatct tttaaaatat gagtaataat tgtacgtgtt
    13261 aataaactac tatcaataga ggctaagtcg ccaccgtacg taattttaat agagcttggt
    13321 gcattgtaca ttaattgaat agctaattca ccaactgttt ggcttaaatt gatgaatgat
    13381 tttacagtat catctatatt gcttaagtcc attttaggtg cattcactgc atgcgttaca
    13441 gtaccatcaa ttaaaatttc gatgatttca tttgaaacag aaattgccac tttttcttga
    13501 gcttcgactg ttgatgcacc caaatgaggt gtaacaataa ttttatcatg tgcaacaaga
    13561 ggcgaatcag ttgcaggttc atgttcaaac acatcgatag ctgcccgact aatttgtcct
    13621 tcgtctaatg cttttattag cgccttttca tcaataatac caccacgtgc cacattgatt
    13681 atttgcaaac taggttttgc tttggcaaaa aagtcagcat taattaagcc ttttgtttta
    13741 ggtgttagtg gtgtatgtaa tgtaacgaaa tcagaatgtt gggcaatctc atcaactgtt
    13801 gccttcgtaa tacttaaaga ttttgctttt tcatccgtta agtaagggtc aaaagctagt
    13861 attttcattc cgaaactttg cgcacgttta gcaacaccta aaccaattct accagcacca
    13921 atgacaccta atgttttatg ataaagctca gtacctttaa atgcatttcg attccattct
    13981 ttatttgtaa gtgattggtg tgcttgcgga atatttcgag ccattgataa taacattgcc
    14041 agtgaatgtt cagtagctga aatcgtatta ccatctgggg cattaataac taaaatacct
    14101 tttaatgttg cagcattaat attaatatta tctacaccaa caccggcgcg tgcgattact
    14161 tttaaagaat cagcagcatt tatgatattt tcagtaaccg tagtttgact acgaacgatt
    14221 aaagcatggt atgaaggtat aattttgatt aatgcttctt cggacaagcc agtttgaata
    14281 tctacattga attgttcgtg atctaataat gctttgatac catcttttga tatgggatct
    14341 gcaacgagta cattaaattg cttcatgaat aacctccata tattttgata tacctttacc
    14401 gatatagtta actttacggt gttcagttaa aataatttct aaagcagata ctaccgataa
    14461 aatatcgaaa ggactaattt tccccatatg accaattctt aaaatttgac ctttaagatg
    14521 gccttgacca ccagcaattg ttattttaaa gcgattttta agttcatctt tgattatttt
    14581 cacttcattt gtattaggtt tgaatgctgt aaccgttgga gatgcatctt tatcattgac
    14641 taataaagtt aaatctaatg cttttaaggc gcttcttaat gcatttctaa ttgcatagtg
    14701 tcgtgctatt acgtgattga aaccttctgc ttttacggtt tcaacgtatg catttacacc
    14761 tctaaataaa gacacatttg gtgtgaacgg tgtagaattg tcagcttgtg acgaaatgta
    14821 tttatttaag tctagataaa attttggcgt agttacttct ttgaaacgtt cttttgcacg
    14881 gtggctataa gctacaaaag ctaatcctgg aggtaacata attgcttttt gactaccaga
    14941 aacaagtaca tcaattttat ctttgttaat gtcaacatca acagcaccaa tgcaacttac
    15001 gccgtcaact acaaaataaa tattactatt aaattgattg atggcatttc ctaactcgtg
    15061 aatagggtgt agcactgtcg tagatgtttc gcaatattga ctaaatactg ctttaacttc
    15121 aacatctaaa gttgaaaggt aattgatgaa atctttaaca tctacagctt ctccccatgt
    15181 tacgtcataa atgtgcacat ttttgtaata agtttgtgca atttgtttaa atcggttacc
    15241 aaaggcacct gaaacaatga caacgaagtg atcttcaggg tttacaatgt tcaacatact
    15301 agcctccaag acgcttgtac cgctagatgt taaaataagt acatcatttt gactcccaaa
    15361 tattggcttt agaccttgaa atgcttgttg tgcgatgtct tcaaaatctt tagaacgatg
    15421 accaaccata ggtgcttgaa tttctctcat aatggcatca ggtacagggg ttgggccagg
    15481 tgttaataac aacggttgat gataatacat ttatatgcct cctataaata agatttcact
    15541 attttagcaa attttctgaa aatttaaaag cctaaaaaaa gtgataacgc tatattgtcg
    15601 aataaacgtt atcacgtaac gattttattt ggcctgaatg atgggataga tttttatgtc
    15661 tacattattt cttacagcat ttgaaatcat gcaattatta tctgcaattg ttatcaattt
    15721 tggtaatcgc ttttctaatt gtgctatttg atcacttgga atttcaatgt gaggatggtg
    15781 cacaattttt gacatactga attttccatt atttaaacaa gctgttccaa tcgattgttg
    15841 ttcaattgaa atatctgtga actttgcacg ttcaagagta gctgctaatg agatgatata
    15901 acatgatgaa gcggctgata ctaacatttc atcgggattt gttcctatac caacaccacc
    15961 taaagaagca ggtattgaaa tattctctga aagtatgtcg ccttgaacgt ttccgacatt
    16021 gttacgacca ccttgccaag aagtttggac tttaaagtca tgttgatgca attgctttaa
    16081 cctccaatat aattgtgata gtttaatttt agaatagttt atcaaagttt aacagaaagg
    16141 tgacttatca atgactctga ataaactgaa agatgaatta caaattgttt cgcaccgtgg
    16201 attaccgagt gattttcctg aaaatacaat ggtcggttat cgagaggtaa tggggctcaa
    16261 tgttgctatg ttagaaatag atgttcattt gaccaaagac caacattttg ttgtgataca
    16321 tgatgaaaca attgatagaa catcggatgg taaggggcgt attgctgatt acacattatc
    16381 gcaattaaaa tcatttgatt ttggtagtta taaagatgtt gcttttaagg gagaacgaat
    16441 acccacgtta gatgaagtac tttcattatg cttaaagtat gataaaaagc tattgattga
    16501 attgaaaagt ccgaatttat atccagaaat agaatgtaaa ttgctggcat ttttggaaga
    16561 aaagaaagtc gatgcgacac aagtagttat acaatctttt gatatcgaat gcatcgaaaa
    16621 gttaaacaca ttgggcagta tatatgaatt aggtgtgctt tgtagtaaac gtaattattg
    16681 gtataaaaag ccaaactttt caagaattgc tcaaattgct agttatgtta atcctaatta
    16741 tgcattagtg gccagaaaat ttgttgataa agcacatcat catcaattac aggttatgcc
    16801 atacacagtt aataaattga aaactggaga aaaattaaga caaatgggtg tagatggatt
    16861 aattacagat aatccgaaat tatttattaa gtgagacgat gaatggtaat ttgatagagg
    16921 gatgaggatt cgtatatcgc aattgataat tttaaaaact aatgatgaaa tactaaagat
    16981 aattaaacaa ataagacttg taaattctaa gttgattgta aaatagggta gctccatata
    17041 attttgaatc tatcattttt aaattaataa aaaaaacacc cacaattgtg ggtgtttgta
    17101 ttgaacgtat tatcttgagt agtactcaac gattaattgt tcgttaattt cagcaggtaa
    17161 ttcgctacgt tctggtaaac gtacgaaagt accagttaag ctgtcagcat caaagtttaa
    17221 gtactcaggt acgaaattgt tgatttcaac tgattcaacg atgatgttta atttttgaga
    17281 tttttcacga actgaaattg tttgaccagg tttaacagaa taagatggaa tatcaacacg
    17341 tttaccatct actaagatat gaccgtggtt aactaattga cgtgcttgac gacgagtacg
    17401 agctaaacct aatgaataaa caacagcgtc taaacgactt gctaataaaa tcatgaagtt
    17461 ttcaccgtgt acaccgaatt ttttaccagc gatgtcaaat gtgttacgga attgtctttc
    17521 agtcattcca tataagtaac gtaatttttg tttttcacgt aattgtaaac catattctga
    17581 taatttttta cgttggtttg gaccatgttg tcctggtgcg taaggacgtt tttctaattc
    17641 tttaccagta ccgcttaaag agatacctaa acgacgagat tttttccagt ttgaacctct
    17701 gaatcgagcc ataataagac tcctccttta attctttttt gttgttatga ataaacaaaa
    17761 aagagtgttg catgcttact aagatatatt gttttatgtg tcttcacctt atagcgacag
    17821 ttacgtgaca cgtccgcatt gggaacaaca tagcgctcaa taatatacaa ctgctacttt
    17881 cgttaattca cacaaagatt attgtaacat tttcctgcta actgtcaaca ctttattgaa
    17941 aatgctgatg tccttttatg cgagttgctt ttcaataatt ttaacaattg cttcaagatg
    18001 ttctttgtca ttgtcatcaa atcgatccgt tataggggca tcgatatcta agacgccgat
    18061 aattttatca tctttgaaaa ttggaacgac aatctcagac ttactattag catcacaagc
    18121 gatatgtcct tcgaattgat ggacatcagc tacaacttgt gtacgacgtt ctgaaacggc
    18181 tgtaccgcat acaccttttc caattggaat gtggacacaa gcggggtgtc cttggaaagg
    18241 tccaagtata agttcatttt gttccaataa gtaaaagcca acccaattaa tttgatttag
    18301 attatcattt aataaggcag acatgttact taaaatcgca atcatatgat gttcatcttc
    18361 aataaggctt gctgcttgtt tctttaataa tgtgtagttt gttgggttaa ttgttgtcat
    18421 atttaaaacc tct
//
LOCUS       DAHHSC010000033        18361 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.033, whole genome shotgun sequence.
ACCESSION   DAHHSC010000033 DAHHSC010000000
VERSION     DAHHSC010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 18361)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18361)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18361
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.033"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            196..1194
                     /locus_tag="NBI57_000885"
     CDS             196..1194
                     /locus_tag="NBI57_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCD1821849.1"
                     /translation="MKKVSIKDVAREAGVSVTTVSHILNHNDSRFSATTIKNVHAVSE
                     RLGYAPNKHAKQLRGSKIQTIGVILPSLTNPFFSALMQSIHDHKPSDVDLCFLTSTAT
                     DLYDNIKHLIDRGIDGLIIAQYISSPDALNNYLKKHHVPYVVLDQNDHQGYTDFVRTN
                     EYQGGQLAAQHLIELGHNHMIIVAPYDMMANMSTRVAGFVDTLRANQLPEPQIVHTEL
                     SKRGGLTIVDDIMVQSATAIFAINDELAIGILRGLIEHGISIPKDISLIGYDDIDYAA
                     YVSPPLTTVAQPITDIGKTSLTLLLQRLQHLDKSIDMIELPTTLKIRATTGYHLSN"
     gene            1426..2307
                     /gene="rbsU"
                     /locus_tag="NBI57_000886"
     CDS             1426..2307
                     /gene="rbsU"
                     /locus_tag="NBI57_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose transporter RbsU"
                     /protein_id="HCD1821850.1"
                     /translation="MSIVALLIGLGPLIGWGFFPTVASKFGGKPVHQIIGATVGTLIF
                     AIILAVVTSSGFPTGTNLLFALLSGAGWGFGQIITFKAFELIGSSRAMPVTTAFQLLG
                     ASLWGVFALGNWPGIGHKIIGFTALVVILIGARMTVWSERKEASNAKNLRRAVVLLLI
                     GEFGYWLYSAAPQATSIDGLTAFLPQAMGMVIVAVIYGFMNMKSENPFRNKITWLQII
                     SGFFFAFGALTYLISAQPNMNGLATGFILSQTSVVLATLTGIYFLKQHKTSKEMVITI
                     IGLVLILVAASVTVFIK"
     gene            2322..2726
                     /gene="rbsD"
                     /locus_tag="NBI57_000887"
     CDS             2322..2726
                     /gene="rbsD"
                     /locus_tag="NBI57_000887"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HCD1821851.1"
                     /translation="MKKSAVLNEHISKAIATIGHFDLLTINDAGMPIPNDHRRIDLAV
                     TKNLPRFIDVLATVLEEMEIQKIYLAEEIKEHNPTQLQQIKQLISSEIEIIFIPHEEM
                     KSNLAHPLNKGNIRTGETTPYSNIALESNVTF"
     gene            2754..3668
                     /gene="rbsK"
                     /locus_tag="NBI57_000888"
     CDS             2754..3668
                     /gene="rbsK"
                     /locus_tag="NBI57_000888"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HCD1821852.1"
                     /translation="MTNKVVILGSTNVDQFLTVERYAQPGETLHVEEAQKAFGGGKGA
                     NQAIATARMQADTTFITKIGTDGVADFILEDFKAAHIDTSYIIKTTEAKTGQAFITVN
                     AEGQNTIYVYGGANMTMTPEDVINAKDAIINADFVVAQLEVPIPAIISAFEIAKAHGV
                     TTVLNPAPAKALPNELLSLIDIIVPNETEAELLSGIKVTNEQSMKDNANYFLSLGIKT
                     VLITLGKQGTYFATKNQSQHIEAYKVNAIDTTAAGDTFIGAFVSRLNKSQDNLADAID
                     FGNKASSLTVQKHGAQASIPLLEEVNQV"
     gene            complement(3766..3900)
                     /locus_tag="NBI57_000889"
     CDS             complement(3766..3900)
                     /locus_tag="NBI57_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1821853.1"
                     /translation="MIIIIINIAYLYAIIWKLKRLQKIVTSFITKHESTSIGLVENYF
                     "
     gene            3919..4680
                     /locus_tag="NBI57_000890"
     CDS             3919..4680
                     /locus_tag="NBI57_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006191654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCD1821854.1"
                     /translation="MSKEAGHTFLAKLGKTRLRPGGKEATDWLIQQGAFSQDKQVLEV
                     ACNMCTTSIYLAHTYGCHIQGVDINKKALEKAQENISAAGLESYIQVQQANAVKLPFD
                     DNQFDIVLNEAMLTMLPIAIKEKALREYYRVLKPGGILLTHDIVIVNESHATHVVKSL
                     SAAINVNVSPQTKLGWLDLYHQAGFNHVHYHTGPMSLMTPKGLIYDEGIVGTIKIIKN
                     ALKKENRPMFCKMFKTMTKLRKDMNYITFVAKKED"
     gene            complement(5057..6493)
                     /locus_tag="NBI57_000891"
     CDS             complement(5057..6493)
                     /locus_tag="NBI57_000891"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HCD1821855.1"
                     /translation="MTKLPQNFMWGGALAANQFEGGYEKGGKGLSVIDVMTSGAHGKA
                     RQITESIDLNHYYPNHEGIDFYHRYKEDIALFKEMGLKCLRTSIAWTRIFPNGDEDVP
                     NEEGLAFYDRIFDELIAQGIEPVVTLSHFEMPLHLAKHYGGFRNREVVDYFVHFARVV
                     FERYKDKVTYWMTFNEINNQMDTSNPIFLWTNSGVALTENDNPEEVLYQVAHHELLAS
                     ALAVRLGKEINPKFKIGTMISHVPIYPYSCHPKDMMEAQIANRLRFFFPDVQVRGYYP
                     SYAKKMLARKGYDVGWQEGDDSILQQGTVDYIGFSYYMSTAVKHDVDTTVENNIVNGG
                     LNHSVENPHIATSDWGWAIDPDGLRYTLNVLYDRYQLPLFIVENGFGAVDEVVDGHIH
                     DDYRIEYLKAHITAAIEAVDQDGVDLIGYTPWGIIDIVSFTTGEMKKRYGLIYVDRDN
                     DGHGTMERLKKDSFYWYQQVIASNGDKL"
     gene            complement(6509..7300)
                     /locus_tag="NBI57_000892"
     CDS             complement(6509..7300)
                     /locus_tag="NBI57_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HCD1821856.1"
                     /translation="MSNKYKEQAQDILTAVGGVENIVDATYDTKCITIHMQHTIPSTA
                     NEVKQIVDVTSVAENDAQLVIKLNGNVDEVYQQLQRLIKNANVEESENTDNINSQDTS
                     YTPQVKVTTPILVKAPIAGRRILLKEVRDSIFREKMVGEGLAIKAHEESKVIAPFNGL
                     ISMIVPTKHAVGIQSEDGVDIVIHIGVNTVDLEGKGFKCFVKQNDHVEAGQTLLQFDQ
                     QYIQQQGYNADVIVVISNSADLGKVELTMNEIITTEDVIFKIFKN"
     gene            7449..8153
                     /locus_tag="NBI57_000893"
     CDS             7449..8153
                     /locus_tag="NBI57_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCD1821857.1"
                     /translation="MLKYEHIAKQLNAFIHQSNFKPGDKLPNVTQLKERYQVSKSTII
                     KALGLLEQDGLIYQAQGSGIYVRNIADANRINVFKTNGFSKSLGEHRMTSKVLVFKEM
                     ATPPKSVQDELQLNADDTVYYLERLRFVDDDVLCIEYSYYHKEIVKYLNDDIAKGSIF
                     DYLESNMKLRIGFSDIFFNVDKLTSSEASLLQLSTGEPCLRYHQTFYTMTGKPFDSSD
                     IVFHYRHAQFYIPSKK"
     gene            complement(8261..8962)
                     /gene="lrgB"
                     /locus_tag="NBI57_000894"
     CDS             complement(8261..8962)
                     /gene="lrgB"
                     /locus_tag="NBI57_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antiholin-like protein LrgB"
                     /protein_id="HCD1821858.1"
                     /translation="MINHLALNTPYFGILLSVIPFFLATILFEKTNRFFLFAPLFVSM
                     VFGVAFLYLTGIPYKTYKIGGDIIYFFLEPATICFAIPLYKKREVLVKHWHRIIGGIG
                     IGTVVALLIILTFAKLAQFANDVILSMLPQAATTAIALPVSAGIGGIKELTSLAVILN
                     GVIIYALGNKFLKLFRITNPIARGLALGTSGHTLGVAPAKELGPVEESMASIALVLVG
                     VVVVAVVPVFVAIFF"
     gene            complement(8955..9398)
                     /gene="lrgA"
                     /locus_tag="NBI57_000895"
     CDS             complement(8955..9398)
                     /gene="lrgA"
                     /locus_tag="NBI57_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antiholin-like murein hydrolase modulator LrgA"
                     /protein_id="HCD1821859.1"
                     /translation="MVVKQQKDASKPAHFFHQVIVIALVLFVSKIIESFMPIPMPASV
                     IGLVLLFVLLCTGAVKLGEVEKVGTTLTNNIGLLFVPAGISVVNSLGVISQAPFLIIG
                     LIIVSTILLLICTGYVTQIIMKVTSRSKGDKVTKKIKIEEAQAHD"
     gene            complement(9511..10251)
                     /locus_tag="NBI57_000896"
     CDS             complement(9511..10251)
                     /locus_tag="NBI57_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor LytR"
                     /protein_id="HCD1821860.1"
                     /translation="MKALIIDDEPLARNELTYLLNEIGGFEEINEAENVKETLEALLI
                     NQYDIIFLDVNLMDENGIELGAKIQKMKEPPAIIFATAHDQYAVQAFELNATDYILKP
                     FGQKRIEQAVNKVRATKAKDDNNASAIANDMSANFDQSLPVEIDDKIHMLKQQNIIGI
                     GTHNGITTIHTTNHKYETTEPLNRYEKRLNPTYFIRIHRSYIINTKHIKEVQQWFNYT
                     YMVILTNGVKMQVGRSFMKDFKASIGLL"
     gene            complement(10254..12008)
                     /locus_tag="NBI57_000897"
     CDS             complement(10254..12008)
                     /locus_tag="NBI57_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014373763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HCD1821861.1"
                     /translation="MLSLTMLLLERVGLIIILAYVLMNIPYFKNLMNRRRTWKARWQL
                     CIIFSLFALMSNLTGIVIDHQHSLSGSVYFRLDDDVSLANTRVLTIGVAGLVGGPFVG
                     LFVGVISGIFRVYMGGADAQVYLISSIFIGIIAGYFGLQAQRRKRYPSIAKSAMIGIV
                     MEMIQMLSILTFSHDKAYAVDLISLIALPMIIVNSVGTAIFMSIIISTLKQEEQMKAV
                     QTHDVLQLMNQTLPYFKEGLNRESAQQIAMIIKNLMKVSAVAITSKNEILSHVGAGSD
                     HHIPTNEILTSLSKDVLKSGKLKEVHTKEEIGCSHPNCPLRAAIVIPLEMHGSIVGTL
                     KMYFTNPNDLTFVERQLAEGLANIFSSQIELGEAETQSKLLKDAEIKSLQAQVSPHFF
                     FNSINTISALVRINSEKARELLLELSYFFRANLQGSKQHTITLDKELSQVRAYLSLEQ
                     ARYPGRFNININVEDKYRDVLVPPFLIQILVENAIKHAFTNRKQGNDIDVSVIKETAT
                     HVRIIVQDNGQGISKDKMHLLGETSVESESGTGSALENLNLRLKGLFGKSAALQFEST
                     SSGTTFWCVLPYERQEEE"
     gene            complement(12253..12927)
                     /gene="scdA"
                     /locus_tag="NBI57_000898"
     CDS             complement(12253..12927)
                     /gene="scdA"
                     /locus_tag="NBI57_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair di-iron protein ScdA"
                     /protein_id="HCD1821862.1"
                     /translation="MINKNDIVADIVIDYPKAADIFRSVGIDFCCGGQVSIEAASLEK
                     KNVDLNELLQRLNDVEQTNTPGSLNPKFLNVSSLIQYIQAAYHEPLREEFKNLTPYVT
                     KLSKVHGPNHPYLVELKETYDTFKSGMLEHMQKEDDVDFPKLIKYEQGEVVNDINTVI
                     DDLVSDHIATGQLLVKMSDLTSSYEPPIEACGTWRLVYQRLKALEVLTHEHVHLENHV
                     LFKKVS"
     gene            complement(13071..14792)
                     /gene="tarS"
                     /locus_tag="NBI57_000899"
     CDS             complement(13071..14792)
                     /gene="tarS"
                     /locus_tag="NBI57_000899"
                     /EC_number="2.4.1.355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000975351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly(ribitol-phosphate)
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HCD1821863.1"
                     /translation="MMKFSVIVPTYNSEKYITELLNSLAKQDFPKTEFEVVVVDDCST
                     DQTLQIVEKYRNKLNLKVSQLETNSGGPGKPRNVALKQAEGEFVLFVDSDDYINKETL
                     KDAAAFIDEHHSDVLLIKMKGVNGRGVPQYMFKETAPEVTLLNSRIIYTLSPTKIYRT
                     ALLKDNDIYFPEELKSAEDQLFTMKAYLNANRISVLSDKAYYYATKREGEHMSSAYVS
                     PEDFYEVMRLIAVEILNADLEEAHKDQILAEFLNRHFSFSRTNGFSLKVKLEEQPQWI
                     NALGDFIQAVPERVDALVMSKLRPLLHYARAKDIDNYRTVEESYRQGQYYRFDIVDGK
                     LNIQFNEGEPYFEGIDIAKPKVKMTAFKFDNHKIVTELTLNEFMIGEGHYDVRLKLHS
                     RNKKHTMYVPLSVNANKQYRFNIMLEDIKAYLPKEKIWDVFLEVQIGTEVFEVRVGNQ
                     RNKYAYTAETSALIHLNNDFYRLTPYFTKDFNNISLYFTAITLTDSISMKLKGKNKII
                     LTGLDRGYVFEEGMASVVLKDDMIMGMLSQTSENEVEILLSKDIKKRDFKNIVKLNTA
                     HMTYSLK"
     gene            complement(14825..16513)
                     /locus_tag="NBI57_000900"
     CDS             complement(14825..16513)
                     /locus_tag="NBI57_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-glycerol glycerophosphotransferase family
                     protein"
                     /protein_id="HCD1821864.1"
                     /translation="MVKSKIYIDKIYWERVQLFVEGHSENLDLEDSNFVLRNLTETRT
                     MKANDVKIDGNQFVCRFNVAILDNGYYLPEDKYLLVNEQELDYIAQLNPDVINDAYQN
                     LKPEQEEEYNELETQNGKINFLLQTYLKEFRKGGISKKTVYTVTPEISSDVNEFVLDV
                     VVTTPEVKSIYIVRKYKELRKYFRKQSFNTRQFIFKAIFNTTKFFHLKKGNTVLFTSD
                     SRPTMSGNFEYIYNEMLRQNLDKKYDIHTVFKANITDRRGIIDKFRLPYLLGKADYIF
                     VDDFHPLIYTVRFRRSQEVIQVWHAVGAFKTVGFSRTGKKGGPFIDSLNHRSYTKAYV
                     SSETDIPFYAEAFGIKEKNVVPTGVPRTDVLFDEAYATQIKQEMEDELPIIKGKKVIL
                     FAPTFRGSGHGTAHYPFFKIDFERLARYCEKNNAVVLFKMHPFVKNRLNIADKHKQYF
                     VDVSDFREVNDILFITDLLISDYSSLIYEYAVFKKPMIFYAFDLEDYITTRDFYEPYE
                     SFVPGKIVQSFDALMDALDNEDYEGEKVIPFLDKHFKYQDGRSSERLVRNLFGS"
     gene            complement(16535..17560)
                     /locus_tag="NBI57_000901"
     CDS             complement(16535..17560)
                     /locus_tag="NBI57_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HCD1821865.1"
                     /translation="MINQVYQLVAPRQFEVTYNNVDIYSDYVIVRPLYMSICAADQRY
                     YTGSRDENVLSQKLPMSLIHEGVGEVVFDSKGVFNKGTKVVMVPNTPTEKDDVIAENY
                     LKSSYFRSSGHDGFMQDFVLLNHDRAVPLPDDIDLSIISYTELVTVSLHAIRRFEKKS
                     ISNKNTFGIWGDGNLGYITAILLRKLYPESKIYVFGKTDYKLSHFSFVDDVFFINKIP
                     EGLTFDHAFECVGGRGSQSAINQMIDYISPEGSIALLGVSEFPVEVNTRLVLEKGLTL
                     IGSSRSGSKDFQDVVDLYIQYPDIVDKLALLKGQEYEIATINDLTEAFEADLSTSWGK
                     TVLKWIM"
     gene            complement(17553..18269)
                     /locus_tag="NBI57_000902"
     CDS             complement(17553..18269)
                     /locus_tag="NBI57_000902"
                     /EC_number="2.7.7.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribitol-5-phosphate cytidylyltransferase"
                     /protein_id="HCD1821866.1"
                     /translation="MKYAGILAGGIGSRMGNVPLPKQFLDLDNKPILIHTLEKFILIN
                     DFEKIIIATPQQWMTHTKDTLRKFKISDERIEVIQGGSDRNDTIMNIVKHIESTNGIN
                     DDDVIVTHDAVRPFLTHRIIKENIQAALEYGAVDTVIDAIDTIVTSKDNQTIDAIPVR
                     NEMYQGQTPQSFNINLLKESYAQLSDEQKSILSDACKIIVETNKPVRLVKGELYNIKV
                     TTPYDLKVANAIIRGGIADD"
BASE COUNT         5899 a         3435 c         2775 g         6252 t
ORIGIN      
        1 atcattaaaa ggtattaacc ttaataatat attctctcgt ctcaacctta atcgtatact
       61 tcagacgtct gtttgtagac aataaaagtc attcacgtct tcatatgtca tcaaatgttt
      121 atcatgatat gatgaatata ataatcgggt atataactgt atgattaatt acacaataaa
      181 gagaaggaat ataatatgaa aaaagtgtca attaaagatg ttgctagaga agctggtgta
      241 tcagttacaa ctgtgtcaca tattttaaat cataatgata gtcgtttttc cgcaacaacg
      301 ataaaaaacg tacatgctgt ttcagaacgt ttaggctatg cccctaataa acatgcaaaa
      361 caattgcgcg gcagtaaaat tcaaactatt ggcgtcattt tacctagctt aacaaatccg
      421 tttttctcag cactgatgca aagtattcat gaccataaac catctgatgt tgatttatgc
      481 tttttaacat ctacagcaac tgatttgtat gacaatatta aacatttaat tgatcgaggt
      541 attgacggat taattatcgc acaatacata tcatccccgg acgccctaaa taactatcta
      601 aagaaacatc atgtacctta tgtcgtactg gatcaaaatg accatcaagg ctatacagat
      661 tttgttcgga caaatgaata tcaaggtgga caacttgcag cacaacattt aatagaactc
      721 ggtcacaacc atatgataat tgttgcacca tatgacatga tggcgaatat gtcgactcgt
      781 gtcgctggat ttgtcgatac tttgcgcgcg aatcaattgc cagaaccaca aatcgtccat
      841 actgaattat ctaagcgcgg tgggctaacc attgttgatg acatcatggt tcaatctgcc
      901 actgcaatct tcgctattaa cgatgaactc gctattggca ttttacgagg actaattgaa
      961 catggcatca gtatcccgaa agatatctca ttaataggtt atgacgacat tgattatgca
     1021 gcgtacgtct cgccaccttt aactactgtg gcacaaccta taactgatat tggcaaaaca
     1081 tctttaacct tattacttca acgattacag cacttagata aatccattga tatgattgaa
     1141 ttaccaacga ctttaaaaat tcgtgcaaca actggctatc atctttcaaa ctaactacgt
     1201 atcttccgaa atatactcat cattgttagg cccttagcgt tgctttaatg ctgagggttt
     1261 ttaatcataa ttatttcact aagaaattaa aataataatg tatgaatttt taaatatgat
     1321 ttaaacgttt tcagttttta tgaaaacgca tgcattttgc aaataaaaat ggtacgatgg
     1381 cattggtaaa acgttttact aaaaacaaat cgtgaggtgt ataacatgag cattgttgca
     1441 ttacttatcg ggttaggccc cttaattggc tggggcttct tcccaacagt cgcttcaaag
     1501 tttggtggta aacctgtaca tcaaattatc ggtgctactg taggtacgtt aatcttcgct
     1561 attatattag ccgtagtcac atcaagtggc ttccctactg gaaccaattt gctattcgcc
     1621 ttattatcag gtgcaggatg gggattcgga caaatcatta catttaaagc gttcgaatta
     1681 atcggctcat ctcgtgccat gccagtcaca acagcattcc aattattagg cgcatcttta
     1741 tggggtgtct ttgcattagg aaattggcca ggcattggtc ataaaatcat tggatttaca
     1801 gctttagtcg ttattctaat tggtgcacgt atgacagttt ggagtgaacg caaagaagca
     1861 agtaacgcca aaaatttacg tcgtgcagtg gtacttctgt taattggtga atttggatac
     1921 tggttatatt cagctgcacc gcaagcaact tctattgatg gcctaactgc ctttttacct
     1981 caagcaatgg gtatggtaat tgttgcagtc atttatggct ttatgaatat gaaatcagag
     2041 aatccattcc gtaataaaat tacgtggtta caaattattt caggtttctt ctttgcattt
     2101 ggtgccttaa catatcttat ttcagcacaa cctaatatga atggtttagc aactggattt
     2161 attctttctc aaacatccgt tgtgcttgct acattaactg gtatttattt cttaaaacaa
     2221 cataaaactt caaaagaaat ggttattaca attatcggct tagtactcat tttagtagcc
     2281 gcttctgtta cagtatttat aaaataagga gtgtagatgt catgaaaaaa tcagctgttt
     2341 taaatgaaca tatttcaaaa gcaatcgcga caattggtca ttttgattta ttaacgatta
     2401 atgacgctgg catgccaatt ccaaatgatc atcgccgtat cgacctagct gtcacaaaaa
     2461 acttaccacg ctttattgat gtcttagcta cagtgttaga agaaatggaa atccaaaaaa
     2521 tatacttagc agaagaaata aaagaacata accctacaca attgcaacaa attaaacaat
     2581 tgatttcatc ggaaatcgaa atcattttca ttcctcacga agaaatgaaa agtaacttag
     2641 ctcacccatt aaataaaggt aatattcgta ctggtgaaac aacgccctac tctaatattg
     2701 cattagaatc gaatgttact ttttaaaaat tataacttga aaggagcata cacatgacca
     2761 acaaagttgt tattttaggt tcaacgaatg tcgatcaatt tttaacagtt gaaagatatg
     2821 cacaaccagg cgaaacatta catgttgaag aagcacaaaa agcattcggc ggaggtaaag
     2881 gtgccaacca ggctattgcc actgcacgca tgcaagcaga cacaacattt attactaaaa
     2941 ttggcactga tggcgttgct gatttcatct tagaagattt taaagcggct catattgata
     3001 catcatatat tatcaaaaca actgaagcaa aaacgggcca agcctttatc actgtgaatg
     3061 cagaaggaca aaacaccatc tatgtttatg gtggtgcgaa tatgacgatg acacctgaag
     3121 atgttattaa cgcaaaagac gctataatca atgcagactt tgtcgttgca caattagaag
     3181 tacccatccc ggctattata tctgcatttg aaattgccaa ggcacatggt gtgacgacag
     3241 tattaaatcc tgcaccagcg aaagcattac ctaatgaatt attatcatta atcgatatta
     3301 ttgtgccaaa cgaaacagaa gccgaattgt tatctgggat taaagtaact aatgaacaat
     3361 ctatgaaaga caatgccaat tactttttat ctttaggcat taagactgtt ttgattacgc
     3421 taggtaagca aggtacatat tttgctacta aaaatcaaag ccaacacatc gaagcttata
     3481 aagtaaatgc gattgataca actgctgcag gcgacacatt tattggtgca tttgtcagtc
     3541 gcttaaacaa gtcgcaagat aacttagcag atgctattga ttttggtaat aaagcgagct
     3601 cactcactgt acaaaaacac ggcgcgcaag catctattcc tctactagaa gaagtaaatc
     3661 aagtttaaat gaatcaaaca cagctatgat atgaaggttt agcatataac atgcaacatt
     3721 cgtatatcat ggctgtgctt ttttatcttt ataaaacatc atctattaga aataattttc
     3781 cactaaacct atgcttgttg actcatgttt agttataaat gaagtgacaa ttttttgtaa
     3841 tctttttaac ttccaaatta ttgcatataa atatgctata ttaatgataa taattatcaa
     3901 ttaaaaggag gttatgctat gtctaaagaa gctggtcata catttttagc taaattagga
     3961 aaaactcgtc tacgccccgg tggtaaagaa gcaacagatt ggttaataca acaaggggca
     4021 ttttcacaag ataaacaagt gttagaagtg gcatgtaata tgtgcacaac atctatttat
     4081 ctagctcata catatggctg tcacattcaa ggcgttgata taaataagaa agcattagaa
     4141 aaagcacagg aaaatatttc agcagcaggt cttgaatcat atattcaagt tcaacaagcg
     4201 aatgctgtta aattgccctt tgatgacaat caattcgata tcgttttaaa tgaagcaatg
     4261 ttaacaatgt tacccatcgc cataaaggaa aaagcattac gcgagtacta ccgagtctta
     4321 aagcctgggg gtatcttgtt aacacatgat attgtcatcg ttaatgaatc acatgccaca
     4381 catgttgtta aatcattatc tgcagcaatt aatgtcaatg tctcaccgca gacgaaactt
     4441 ggctggttag atttatatca tcaagctggt tttaatcatg tgcattatca tactggtcca
     4501 atgagtttaa tgacaccaaa aggtttaatt tatgacgaag gtattgttgg aactataaag
     4561 attatcaaaa atgctttaaa aaaagaaaat cgaccaatgt tttgtaaaat gtttaaaacg
     4621 atgactaaat tgcgtaaaga tatgaattat attacttttg tcgctaaaaa agaggactaa
     4681 atataatgcc actaactgta ctttgtatct atgtttgact gctactttaa tttctttgtg
     4741 acactaatca tctacttaac aatatcgtta tcgttgatta gtaagtcatc aatttgggtt
     4801 aaagactttc ataaacactc aaacattaac actatactta gttagtggca ttattttttt
     4861 aaaaatttta acatcacggg attggaacag aaatgatatt ttcgcaaaat ttattcctgt
     4921 ccttccccaa cttgcattac ctgtagaatt tcttttcgaa attctttgtg ttgggggccc
     4981 gccattataa cactgtaggt cctactatat taatttatgt ctcgggctct aaccctaaaa
     5041 tacttataat atacctttat aatttatctc catttgatgc tatcacttgt tgataccaat
     5101 agaacgaatc ttttttcaag cgttccatcg tgccatgacc atcattatct cgatcaacat
     5161 atattaaacc atagcgtttc ttcatttcac cggttgtaaa tgaaacaata tcaatgattc
     5221 cccacggtgt ataaccgatt aaatctacac catcttgatc aactgcttct atcgctgctg
     5281 taatatgtgc ttttaaatat tcaatgcgat aatcatcatg aatatgtcca tctaccactt
     5341 catcaactgc accaaaacca ttttccacaa taaaaagtgg taactgataa cgatcatata
     5401 acacattcaa tgtatatctt aagccatctg gatcaatcgc ccaaccccaa tcactcgttg
     5461 cgatatgcgg attctccaca gaatgattca aaccaccgtt gacgatgttg ttttcaactg
     5521 tagtatcaac atcatgtttt acagccgtag acatgtaata actaaagcca atataatcaa
     5581 ccgtgccctg ctgtaaaata ctgtcgtccc cttcttgcca tccaacatca tatccttttc
     5641 gtgccaacat ttttttagca tagcttggat aataacctct cacttggaca tccgggaaaa
     5701 agaaacgtaa gcgattcgca atttgtgctt ccatcatatc tttcggatga cacgaatatg
     5761 gataaatggg tacatgtgaa atcattgttc caatcttaaa cttcggatta atctctttac
     5821 caagacgaac tgctaaagca ctggctaaaa gttcatgatg tgctacttga tacaagactt
     5881 cttcaggatt atcattttct gtcaatgcta ccccagaatt cgtccataaa aagataggat
     5941 ttgatgtgtc catctgatta ttaatttcat taaacgtcat ccaatatgta actttatctt
     6001 tatatctttc aaatacaaca cgcgcaaaat gcacaaaata atcgacaact tctctatttc
     6061 taaatccacc ataatgtttc gctaaatgaa gtggcatctc aaaatgtgat aacgtcacaa
     6121 caggttcaat accttgtgca attaattcat caaagatacg atcataaaag gcaagtcctt
     6181 cttcatttgg cacatcttca tccccattcg gaaagatacg tgtccacgca atcgacgtac
     6241 gtaaacattt caatcccatt tctttaaaca aggcaatatc ttccttataa cgatgataaa
     6301 aatcaatacc ttcatgattt ggataatagt gattgaggtc tatagattct gtaatctgac
     6361 gtgctttgcc atgtgcacca ctcgtcataa catcaattac acttaaccct ttaccacctt
     6421 tttcatatcc accttcaaat tgatttgcgg caagagcgcc accccacata aaattttgcg
     6481 gtaattttgt cattattaca acacactcct agtttttaaa tattttaaaa ataacatctt
     6541 cagtcgtaat gatttcattc attgtcagtt ctacttttcc taaatcggca gagttgctaa
     6601 taacgacaat aacgtcagca ttgtagcctt gttgttgtat atattgctgg tcgaattgca
     6661 acaacgtttg ccctgcttca acatgatcat tttgctttac aaagcacttg aaccctttac
     6721 cttccaagtc aactgtattc acgccaatat gaatgactat gtccacaccg tcttctgatt
     6781 gaataccaac tgcatgctta gttggtacaa tcatagatat taaaccattg aacggtgcga
     6841 ttactttgga ttcttcatga gctttgattg ctaagccttc acctaccatt ttctctctaa
     6901 aaattgaatc tcttacttct ttaagtaaaa tacgacgacc agcgattggt gctttcacta
     6961 aaattggtgt tgttactttt acttgaggtg tataacttgt atcttggcta ttaatattgt
     7021 cagtattctc actctcttcg acattagcat tcttaattaa tcgctgtaat tgctgataca
     7081 cttcatcgac atttccattt aattttatga ctaactgcgc atcattttct gctacagatg
     7141 tcacatcaac tatttgtttc acttcatttg ctgtagaagg aattgtatgt tgcatatgaa
     7201 ttgtaatgca cttcgtatca tacgttgcat caacaatgtt ttcgacacca cctacagctg
     7261 taagaatgtc ttgggcttgt tctttatatt tattgctcat aaatattacc ccctcaactt
     7321 gatagtagaa tgaaaatgta tttacttacc tccaattata attgtaccgg ttcaatttgt
     7381 aaacgccgat acaattataa tattttgtgc tataataatt acagacaaag tgaaacgagg
     7441 acagaatatt gttaaagtat gaacatattg ctaagcaact taatgcgttt atacatcaat
     7501 ctaatttcaa acccggtgat aaattgccaa acgtgacgca attaaaagaa cgttatcaag
     7561 taagtaagag tactatcatt aaagcattag gcttattgga acaagatggt ttgatctatc
     7621 aagcacaagg cagtggtatt tatgtgagaa atattgcaga tgccaatcgt atcaacgtct
     7681 ttaagactaa tggtttctct aaaagtttag gtgaacatcg aatgacaagt aaggtacttg
     7741 tttttaagga gatggcaacg ccacctaaat ctgtacaaga tgaactccaa ttaaatgcag
     7801 atgataccgt ctactattta gagcgattaa gattcgtgga cgatgatgtt ttatgtatcg
     7861 aatattctta ttatcataaa gaaatcgtga aatatttaaa tgatgatatt gctaagggct
     7921 ctatcttcga ttatttagaa tcaaacatga aacttcgtat tggtttttca gatattttct
     7981 ttaatgtaga taaactcact tcaagtgaag cttcattact acaattgtct acaggtgaac
     8041 catgtttacg ttaccaccag actttttata caatgactgg caaacccttt gattcgtctg
     8101 acatcgtatt tcattatcgt catgcacagt tttatattcc tagtaaaaag taataaatac
     8161 ataaaaacgt ctatatccca gttataaact ggagtataga cgttttttta cgataataac
     8221 aatggctcaa attgctatta tcttgcttag gtttttcgtt ttagaagaat attgctacaa
     8281 agacaggcac aactgctaca acaactacac caactaacac taaagctata cttgccattg
     8341 attcttctac aggtcctaat tctttggctg gtgctacacc taatgtgtga ccacttgttc
     8401 caagtgctaa tcctcgggca atagggttag taattcggaa aagcttcaag aatttattac
     8461 ctagggcata aataatgaca ccatttaaaa taactgctaa tgatgttaat tcttttatac
     8521 caccgatacc agctgatact ggtaacgcaa tcgctgtagt tgctgcttga ggtaacattg
     8581 ataaaataac atcattggca aattgtgcta acttcgcaaa agttaaaata attaataacg
     8641 ctacaactgt accgatacca atacctccta tgatacgatg ccaatgttta acaagcactt
     8701 cacgcttttt atataacgga atcgcaaaac agattgttgc cggttctaag aagaagtaaa
     8761 taatgtctcc acctattttg taagtcttat acggaatgcc tgttaaatag aggaaggcca
     8821 caccaaatac catactgaca aatagcggtg cgaataagaa gaaacgatta gttttttcaa
     8881 ataatatggt cgctaagaaa aatggtataa cggataacag tattccgaag taaggtgtgt
     8941 ttagtgctaa gtggttaatc atgagcttgt gcctcctcta ttttgatctt ttttgtgact
     9001 ttgtcacctt tagatctcga agtaactttc ataataattt gtgtgacata gccagtacaa
     9061 ataagtaata gtattgttga gacgattatt agtccaatga ttaaaaatgg tgcttggcta
     9121 atgacaccta aagagttaac aactgagata ccggctggta cgaagagtaa gccaatgtta
     9181 tttgttagtg tcgttcctac tttttcgact tcgcctaact taacagcacc agtacataat
     9241 aatacaaata ataatactaa accgattact gatgcaggca taggaattgg cataaatgat
     9301 tcaattattt tcgatacaaa gagtactaaa gcaattacaa tgacttggtg aaaaaagtgt
     9361 gctggttttg atgcgtcttt ttgttgtttc acgaccattg cctcctacgt ttgatttaac
     9421 taaagtatag atggctcact tcgatttgcg tgatttttag tccgaaatac aaaatatcat
     9481 aggtaaaatg cataaaaaaa aggattactg ttaaagtaat cctatcgacg ctttaaaatc
     9541 tttcataaat gaacgtccaa cttgcatctt gacaccattt gtcaatatta ccatataagt
     9601 gtagttaaac cattgttgca cttctttaat gtgtttcgtg ttaataatat atgaacgatg
     9661 aatacgtata aaataagtgg gattcaatcg tttttcataa cgattcaatg gctctgttgt
     9721 ttcgtattta tgattcgttg tatgtatggt tgtaatacca ttatgtgtgc caatcccaat
     9781 aatattttgt tgctttaaca tgtgaatttt atcgtcaatt tcaacaggta agctttgatc
     9841 aaaattcgcc gacatatcat tcgcaattgc acttgcgtta ttatcatctt tggctttagt
     9901 cgcacgcact ttattgactg cttgttcaat acgtttttga ccaaacggtt tcaaaatata
     9961 gtctgtcgca tttaattcaa atgcctgtac tgcgtattgg tcatgtgcag ttgcaaaaat
    10021 aatcgcaggt ggctctttca tcttttgaat cttagctcct aattcgatcc cattttcatc
    10081 cattaaattg acatctaaaa atataatgtc atattgattg atcagtagtg cttccaatgt
    10141 ttcttttaca ttttctgcct cattaatttc ttcaaaacca ccaatttcat ttaataaata
    10201 tgttaattca ttacgtgcta atggctcatc atctatgatt aatgctttca tatttattcc
    10261 tcctcttgtc tttcataagg aagtacacac caaaaagtgg taccgctcga tgtcgattca
    10321 aattgtaatg ctgcggattt tccaaataat ccttttaggc gtaagtttaa attttctaaa
    10381 gcactaccag ttccagattc tgattctaca gatgtttctc ccaacaaatg cattttatct
    10441 ttagaaatac cctgaccatt atcttgtaca ataatacgta catgtgttgc agtttcttta
    10501 atcactgaca cgtcaatatc gttaccttgc tttcgattcg taaacgcatg tttgatggca
    10561 ttttcaacta aaatttgaat taaaaatggt ggtacaagca catcgcgata tttgtcttca
    10621 acattaatat tgatattaaa tcttcctgga taacgtgctt gttcgagtga taagtatgca
    10681 cgcacttgac ttaactcttt atctaaagta atcgtatgtt gctttgagcc ttgtaaattc
    10741 gctctgaaaa aataactcaa ttctaatagt aactctcgtg ccttttcgct atttattcta
    10801 actaaagctg aaatcgtgtt aattgaattg aagaaaaaat gtggactcac ttgtgcctgt
    10861 aatgacttaa tctcagcatc tttcaataac ttactttgcg tttcggcttc accaagttca
    10921 atttggctac taaaaatatt tgccaatcct tctgcaagtt gacgttccac aaaagttaaa
    10981 tcattagggt ttgtaaaata catcttcaat gtaccgacga tagaaccatg catctcaagt
    11041 ggtatcacga tagctgctct aagcgggcaa ttcggatgac tacaaccaat ctcttcttta
    11101 gtatgcactt ctttcaactt tcctgatttc aatacatctt tagacagact tgttaatatt
    11161 tcatttgttg gtatgtgatg atcactacct gcacctacat gcgataagat ttcatttttg
    11221 cttgtaattg ctacggcaga tactttcatt aaatttttaa taatcatcgc aatttgctgt
    11281 gccgattctc tattcaatcc ttctttaaaa tacggcaatg tctggttcat caattgcagt
    11341 acgtcatgtg tttgaacagc cttcatttgc tcttcttgct ttaatgttga aatgataata
    11401 gacataaaaa tcgccgtacc aacgctatta acaataatca ttggtagtgc aattaatgat
    11461 atgaggtcaa ccgcatatgc tttgtcgtgg gaaaatgtta aaatgctcaa catttgaatc
    11521 atttccataa caattccaat catggcactt ttcgcaatac tcgggtaacg cttgcgtctt
    11581 tgagcttgta agccaaaata accagcaatt ataccgataa atatagatga gataagataa
    11641 acttgtgcat ccgccccacc catatacact ctgaaaatac ctgaaataac gccaacaaat
    11701 agacctacaa aagggccacc aactaatcct gcgacaccta tcgttaatac acgtgtgtta
    11761 gctaaagata catcatcatc taaacggaag tacacacttc ctgacaaact atgttgatga
    11821 tcgatgacga taccagttaa attagacatt aaggcaaaca aactgaaaat aatacataat
    11881 tgccaacgtg ctttccatgt acgtcgacga ttcattaagt ttttaaaata tggaatattc
    11941 atcaacacat aggccaaaat aataattaaa cctacacgct caagtaataa cattgttagc
    12001 gatagcacga tagcacctca gtgaattata attatgttta ttttatcata atagtgcatg
    12061 taaaattcat gaataaatta ttaaaaaaac acgctactat ttaattatct ataccattaa
    12121 attttcatat caatttttat gctaagttga ccatttttct tttcaaataa tgctgttcat
    12181 atcactaaat ttctgttaag tatagcgaaa aactgctgac attactttgc caacagtttc
    12241 taatcgcgtt atttatgata cttttttaaa taaaacatga ttctctaaat gaacatgctc
    12301 atgtgttaac acttcaagtg cttttaatct ctgataaacg agtcgccacg taccacatgc
    12361 ctctatcggt ggttcatagc tagatgttaa atcgctcatt ttcactaaca attgtcccgt
    12421 tgcaatgtga tcagatacta aatcatcaat cactgtatta atatcgttta ctacttcacc
    12481 ttgttcatat ttaattagtt ttggaaaatc aacatcatct tctttttgca tatgctctaa
    12541 catgccactt ttaaatgtat catatgtttc ttttaactcg actaaatatg gatggttagg
    12601 tccatgtact ttcgataatt tcgtcacata aggtgttaaa tttttaaatt cttctctaag
    12661 aggttcatga tatgctgctt gaatatattg aataagtgac gaaacattta aaaatttagg
    12721 gttaagcgaa cctggtgtat tcgtttgttc aacgtcattg agacgctgta ataattcgtt
    12781 caaatctaca tttttctttt ctaaggatgc tgcttctata cttacttgtc caccgcaaca
    12841 aaagtctata cctacgcttc taaaaatatc tgctgctttc ggataatcta ttactatatc
    12901 tgctactatg tcatttttat ttatcattat agacgacctc ctcattttca gtgtatacaa
    12961 tttaatgtat atgaaaatga aaatcaggga aatgtcataa tcatttcata aatagcaaaa
    13021 taaaaccctc aaattatgtt agttcacata gatttgaggg catttattat ttattttagc
    13081 gagtaagtca tatgtgcagt gtttaactta acaatatttt tgaagtctcg cttcttaata
    13141 tctttactaa gtaagatttc cacttcgttt tctgacgttt ggcttaacat tcccataatc
    13201 atgtcgtctt ttagcacgac actagccata ccttcttcaa atacataacc acgatccaga
    13261 ccagttaaaa tgattttgtt tttacctttt aacttcattg agattgaatc cgttaatgta
    13321 atagctgtaa agtataacga aatgttatta aagtcttttg tgaaatacgg tgttaatcta
    13381 taaaaatcat tatttaaatg aattaatgca cttgtttctg cagtatatgc atatttatta
    13441 cgttgattac caacacgcac ttcaaatact tccgtaccta tttggacttc taagaaaaca
    13501 tcccaaattt tttctttagg taaatacgct ttaatatctt ctaacataat gttaaaacga
    13561 tattgtttat tcgcattgac acttaaaggt acatacattg tgtgcttctt gtttcgtgaa
    13621 tgtaatttaa gtctgacatc ataatgtcct tcgccaatca taaattcatt taacgttagc
    13681 tctgtaacaa ttttatgatt atcaaattta aatgctgtca ttttcacttt tggcttagcg
    13741 atatcaatgc cttcaaagta tggttcgcct tcattgaatt gaatgtttaa tttaccatct
    13801 acaatatcaa aacggtagta ttgaccttga cggtaacttt cttctacagt tctatagttg
    13861 tctatatctt tcgctctcgc gtagtgcaac aatggtcgta atttactcat caccaatgca
    13921 tctacacgtt ctggaactgc ttgtataaag tctcctagag cattaatcca ttgtggttgc
    13981 tcttcaagtt taactttaag tgagaagcca ttcgtacgag aaaaactaaa atgacgattt
    14041 aaaaattctg ctaagatttg atctttatga gcttcttcta aatctgcatt taatatttct
    14101 acagcaatca atctcatgac ttcgtaaaag tcttcaggtg aaacatacgc actactcata
    14161 tgttcacctt cacgctttgt agcataataa tacgctttat cacttaacac actgattcga
    14221 tttgcattca aatatgcttt cattgtaaat aattgatctt ctgcactctt taattcttct
    14281 ggaaaataaa tgtcattatc ttttagtaat gctgttctat agattttagt cgggcttaaa
    14341 gtatagataa ttcttgaatt taacaaagta acttcaggtg ctgtttcttt aaacatatat
    14401 tgtggtacac cacgaccatt aacacctttc attttaatca ataagacatc tgagtgatgt
    14461 tcatcaataa atgctgctgc atcctttaaa gtctctttgt ttatatagtc atcggagtcc
    14521 acaaataata caaattcacc ttctgcttgt tttaacgcca catttctagg tttacctgga
    14581 ccaccagaat ttgtttcgag ttgacttact ttcaagttca atttattgcg atacttttca
    14641 actatttgta acgtttgatc tgttgaacag tcatcaacta caaccacttc aaattcagtt
    14701 ttcggaaaat cttgtttcgc aaggctatta agtaattctg ttatatactt ttctgaattg
    14761 tatgttggaa ctattactga aaatttcatc attatacctc tcccactttg actactatat
    14821 aaacttagct accaaataaa tttctgacta aacgctcact tgatcggcca tcttgatatt
    14881 taaaatgttt atctaagaat ggaatgactt tttctccttc ataatcttca ttgtccaagg
    14941 cgtccattaa tgcgtcaaat gattgcacaa ttttacctgg aacaaatgat tcatatggtt
    15001 cataaaaatc acgcgtcgta atataatctt ctaaatcaaa tgcatagaaa atcattggct
    15061 ttttaaatac tgcatattca tatattaaag atgaatagtc actaattaat aaatctgtta
    15121 tgaacagtat atcattaact tctctaaagt cagaaacgtc aacaaaatat tgtttatgtt
    15181 tgtctgcaat attaagtcta tttttcacaa atggatgcat tttaaataat acaaccgcgt
    15241 tatttttttc gcaatatctt gctaaacgtt cgaaatcaat tttgaaaaat gggtaatgtg
    15301 ctgtaccatg accactacct ctaaatgttg gtgcgaaaag aatgactttc ttacctttaa
    15361 taattggtaa ttcatcttcc atctcttgtt tgatctgtgt cgcataagct tcatcaaata
    15421 gtacatcagt acgtggaaca cctgtaggca ctacattttt ctctttaata ccaaatgctt
    15481 cagcgtagaa tggaatatcg gtttcagatg atacataagc ttttgtatag ctacgatgat
    15541 ttaatgaatc aataaatggt ccaccctttt taccagtacg actaaagcca actgttttaa
    15601 aggcaccaac ggcatgccat acttgaataa cttcttgaga acgtctaaaa cgcactgtat
    15661 aaatcaatgg gtgaaagtca tcaacaaaga tgtagtctgc cttcccaagt aaatatggca
    15721 atctaaactt gtcgatgatg ccacgtctat ctgtaatatt cgctttaaaa acagtgtgaa
    15781 tatcatactt tttatctaaa ttttgacgta acatttcgtt atagatgtat tcaaagtttc
    15841 cagacatcgt tggtctagag tctgatgtga acaacaccgt attccctttt ttcaagtgga
    15901 aaaatttcgt cgtattaaat atcgctttaa aaataaattg tcttgtatta aatgattgtt
    15961 tgcggaaata cttacgtaat tctttatatt tacgaacgat ataaatactt ttaacttccg
    16021 gagtcgttac aacaacatca aggacaaatt cattaacatc gctagaaatt tcaggtgtaa
    16081 cagtataaac cgttttcttc gaaatgccgc cttttctaaa ttcttttagg taagtctgca
    16141 ataagaaatt gattttacca ttttgtgttt ctaattcgtt gtattcttct tcttgttctg
    16201 gctttagatt ttgatatgca tcattaatca catctgggtt taactgtgca atataatcaa
    16261 gttcttgctc attcactaat aagtacttat cttcaggtaa gtaataacca ttatctaaga
    16321 tagctacatt gaaacgacaa acgaattgat tcccatctat tttgacatca ttcgccttca
    16381 ttgtacgtgt ctcagttaaa tttcttaata caaaattact atcttctaaa tctaggtttt
    16441 cactatgtcc ttcaacgaat aactgaacac gttcccaata gattttatct atatatatct
    16501 tacttttaac caacgttaat tcatcctttt ctatttacat aatccatttt aatactgttt
    16561 taccccaaga tgtagacagg tctgcttcaa aagcttctgt aagatcatta attgttgcaa
    16621 tttcatattc ttgacctttt aacaacgcta atttatctac aatatctggg tattgaatgt
    16681 ataagtctac aacatcttgg aaatcttttg aaccacttcg actactacca atcaacgtta
    16741 gtcctttttc caatactaga cgtgtattaa cttctactgg gaactcactt acacctaaca
    16801 gtgcaatgct tccttctggt gaaatataat caatcatttg atttattgct gattggctac
    16861 cacgcccacc aacgcactca aatgcatgat caaatgttaa gccttcaggt attttattaa
    16921 taaagaagac atcatcaaca aatgagaagt gactcaattt ataatctgtt ttaccaaaga
    16981 catatatttt agactctgga tataatttac gtaataaaat ggctgtaatg taacctaagt
    17041 taccatcacc ccaaatacca aatgtatttt tatttgaaat agatttcttt tcaaaacgac
    17101 gaatagcatg caaacttact gttacaagct ctgtatatga aataatactt aaatcaatat
    17161 catcaggtag tggtacagct ctatcatgat ttagcaacac aaaatcttgc ataaacccat
    17221 catgtccact tgatctgaag tagctcgatt ttaaatagtt ttcagcaatg acatcgtctt
    17281 tttctgtcgg cgtattcggt accataacta cttttgtacc tttattaaac acacctttac
    17341 tgtcaaatac gacctcacca acaccttcat gaattaaaga cattggcaat ttctgagata
    17401 agacattctc atcacggcta ccagtataat atctttgatc ggcagcacaa attgacatat
    17461 ataaaggacg tacaatgaca tagtcactgt aaatatctac gttgttatac gtaacttcaa
    17521 attgtctagg tgcaactaat tgatatactt gattaatcat cggcaatacc acctcgaata
    17581 atagcattcg ctacttttaa atcgtaaggt gttgttactt taatgttata taactcacct
    17641 tttacaagtc gaaccggttt gtttgtttct acaataatct tacaagcatc agataaaata
    17701 ctcttttgct catcactcaa ctgtgcatag ctttctttta ataaattaat attaaacgat
    17761 tgaggtgttt gaccttggta catttcatta cgcactggaa ttgcatcaat cgtttgatta
    17821 tctttagatg taacaatcgt atctatagca tcaatcactg tatctactgc accgtactct
    17881 aaagcagctt gaatattttc tttaataata cgatgcgtta aaaatggtct aactgcatca
    17941 tgtgtcacaa tgacatcgtc atcgttaata ccatttgttg attcaatatg tttaacgata
    18001 ttcataattg tatcgttacg atcgctacca ccttgaatga cttcaattct ttcatcagaa
    18061 attttgaatt ttctaagtgt atctttcgta tgcgtcatcc attgttgtgg cgtcgcgata
    18121 ataatttttt caaaatcatt aattaaaata aatttttcta atgtatggat taaaatcggt
    18181 ttgttgtcta aatctaaaaa ttgtttaggt aaaggtacgt tacccattct tgagcctata
    18241 cctccagcta gaataccagc gtatttcata aaatacttcc tccattcaac tatatctata
    18301 tttaattatt taaatttcgt tgcattttcc aattgaaaac tcattttaaa atcaaaactc
    18361 t
//
LOCUS       DAHHSC010000034        17893 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.034, whole genome shotgun sequence.
ACCESSION   DAHHSC010000034 DAHHSC010000000
VERSION     DAHHSC010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 17893)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17893)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17893
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.034"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            286..1158
                     /locus_tag="NBI57_000903"
     CDS             286..1158
                     /locus_tag="NBI57_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HCD1821867.1"
                     /translation="MSNVLTEIDSQYPYMTKNEKKIAKFILNSPQKVIKMRSQDLASL
                     LDISTSSVIRFSKKITDGGFHDLKINISKYVPKASSIYNVELMNNESTESLRTKLHTR
                     TTRALNHANNELNDKTIDQICHCLKRSETIFIYGFGASFVVATDLYQKLSRIGLNIQL
                     VQETHIFATLLATHNSNDSVILITNNGTQSEMQSMVKVIDDYHIPIITITSTRDNPVA
                     QASNIVLTYGKTDENEMHMGATTSLFAQMFTIDILYYRYVALNYHASLDFITQSKMAL
                     DNYRKHLSNINFKH"
     gene            complement(1545..2924)
                     /locus_tag="NBI57_000904"
     CDS             complement(1545..2924)
                     /locus_tag="NBI57_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCD1821868.1"
                     /translation="MENNELQRGLSARQIQMIALGGTIGVGLFMGATSTIKWTGPSVI
                     LAYLIAGIFLFLIMRAMGEMIYLNPTTGSFATFASDYIHPAAGYMTAWSNIFQWIVVG
                     MSEVIAVGEYMKFWFPELPTWIPGVIAILLLMAANLFSVKAFGEFEFWFALIKVVTII
                     LMIIAGFGLIFFGFGNGGHAVGISNLWTNGGFMPNGIVGFFFALSIVIGSYQGVELIG
                     ITAGETKDPQKNIVKAVNGVIWRILIFYLGAIFVIVSVYPWNQLGDIGSPFVATFAKI
                     GITFAAGLINFVVLTAAMSGCNSGIFSASRMIYTLAHKGEMPKIFTKIMRNGVPLYTV
                     VAVSLGILIGALLNVILPLYIDGAKSIFVYVYSASILPGMIPWFMILFSHLRFRKLHP
                     EELEGHPFKMPGGAVTNYLTILFLILVLVGMVFNVETRISVLIGVIFLTIVTIYYFIR
                     YNKNNVKAK"
     gene            3204..3560
                     /locus_tag="NBI57_000905"
     CDS             3204..3560
                     /locus_tag="NBI57_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1821869.1"
                     /translation="MLKQILPRATKISILFAVAFFIINYIGMEKPDILYLVGRTIIAT
                     LAFILICLTLFTIINSPERKIKLGTTLPIALIIGIIFGAIFLTVQIGVITGLIIGVIA
                     TFIWELIEKNKGGRSS"
     gene            3557..3742
                     /locus_tag="NBI57_000906"
     CDS             3557..3742
                     /locus_tag="NBI57_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1821870.1"
                     /translation="MTTALIGVIVLILLIISFVPNYRAMKSAKEQGSNATRATIMVGI
                     DIILIVLIIVTLLLKYM"
     gene            complement(3838..4029)
                     /locus_tag="NBI57_000907"
     CDS             complement(3838..4029)
                     /locus_tag="NBI57_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1821871.1"
                     /translation="MMGYIILFFLAGPVILGVGNLVIGPIFNKQTPFRVQVRSFVVGS
                     MIYLILATIGYFLLLQGKL"
     gene            complement(4040..4675)
                     /locus_tag="NBI57_000908"
     CDS             complement(4040..4675)
                     /locus_tag="NBI57_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HCD1821872.1"
                     /translation="MYRAVIFDFDGTIIDTEQHLFNVINKHLEMHNADPISIDFYRSS
                     IGGAATDLHDHLIKAIGSENKDKLYEEHHLTSTTLPMIDTIKSLMAFLKQRHIPMAIA
                     TSSVKAEIMPTFKALGLDDYIEVVVGREDVEQVKPDPELYLSAVQQLNYMPTQCLAIE
                     DSVNGATAAIAAGLDVIVNTNKMTSAQDFSNVDYVAKDIDYDQIVARFFTK"
     gene            complement(4750..5667)
                     /locus_tag="NBI57_000909"
     CDS             complement(4750..5667)
                     /locus_tag="NBI57_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter family protein"
                     /protein_id="HCD1821873.1"
                     /translation="MFQKLSIFATKSFLVWMLVAAVIGFIFPQHVATLGKWVPYLLGI
                     VMLGMGLTITPNDFKMVFKAPRAVIIGVCLQFSIMPTLAFIIAKSFHLPPDIAVGVIL
                     VGCCPGGTSSNVMSYLAKANVALSVSITTVSTLLAPFVTPALIYLFANEWLEVSFLSM
                     LWSVVQVVLIPIALGIVLQIINRKIAEKASTALPIISVVAISLILAIVVGGSKHQILT
                     TGLLIFLVVILHNVLGYTIGYWLARLLKLDRQDQKAVSIEVGMQNSGLAVSLAALHFN
                     PIAAVPGAVFSFIHNITGPILAKYWSKKL"
     gene            5935..6465
                     /locus_tag="NBI57_000910"
     CDS             5935..6465
                     /locus_tag="NBI57_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1821874.1"
                     /translation="MLIDKFETYIINIAGLNDRTTRKKLSKLCKSVQFCDALQFSINK
                     QFNQYVLEISLPKQQLPYFISFLSFHQYSIFQVLSPKKINELLDSDNLYQSAKRFDIN
                     IDGLQDAFIKDKVIDIMNMFQNHTDITYTLNKSHAHIICTPEIFAKLLHTIATRNIDI
                     LSANYRSSSMSKARIS"
     gene            complement(6531..8135)
                     /locus_tag="NBI57_000911"
     CDS             complement(6531..8135)
                     /locus_tag="NBI57_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HCD1821875.1"
                     /translation="MNAIKRFGSAMIVPVLMFAFFGIVLGFATLFKNPTIMGSLADQH
                     TFWFKFWSVIESGGWVIFTHMEVVFVVGLPLSLAKKAPGHAALAALMGYLMFNTFINA
                     ILTQWPHTFGANLEKGVENVPGLKSIAGIATLDTNILGGIIISAIITWIHNRYYSKRL
                     PEMVGVFQGLTFVVTISFFVMLPLAAITCVIWPTVQHGIGSMQHFIIASGYIGVWLYH
                     FLERVLIPTGLHHFIYAPIEVGPVVVNHGLKAEWLQHLNEFAKSTKPLKEQFPYGFML
                     QGNGKVFGCLGIALAMYATTPKENRKKVAALLIPATLTAVVVGITEPLEFTFLFIAPY
                     LFVLHAVLAASMDTLMYAFGVVGNMGGGLLDFISTNWLPLGKEHWGTYVAQVIIGLIF
                     VAIYFFLFRFLILKFDIPLPGRKKTEEEVKLFSKQDYKNKKGDSVDSKRASTGNEYED
                     KAAYYLDGLGGKENIKDVTNCTTRLRLTVYDESKVADTEYFTHQQMAHGLVKSGKSIQ
                     VVVGMTVPQVREAFEQMVEDQSSEDK"
     gene            8465..9229
                     /locus_tag="NBI57_000912"
     CDS             8465..9229
                     /locus_tag="NBI57_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HCD1821876.1"
                     /translation="MFLDEHINRNFDKLNDNDLHIAHFINTHIDECKNMKIQDLAQFT
                     HASNATIHRFTRKLGFDGYSDFKSYLKFESNKTHQLPSNSIDSFKQEIDSTFSYLERV
                     DYQLLTEKMHTASTIYLYGTGRAQLNVASEAQRILLTLHNHVIVLHDEHELKMILNKS
                     EPNDLFFIISLSGETHELLEVTNLLQLRQKYFISVTTMKDNTLAQKANYNVYVSSNTF
                     YLTDGTDYSSFISYHIFFETLVRKYNEYLLHNDISN"
     gene            complement(9287..9793)
                     /locus_tag="NBI57_000913"
     CDS             complement(9287..9793)
                     /locus_tag="NBI57_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HCD1821877.1"
                     /translation="MAKFNVENEHVEVEIEKLYKFSPELVYEAWTKKDLLKQWFMTSA
                     RTNKEIEADVKEGGKYRIVDQQRNGKVNVIEGIYESLVMDEYVKMTIGMPGLSETQDV
                     IEVEFFERETGGTQMLFYYRSLVEKERRFTNLEYKQKKKEYHDAMVHGFELMFDKMYH
                     VIETSTQQ"
     gene            complement(9960..11273)
                     /locus_tag="NBI57_000914"
     CDS             complement(9960..11273)
                     /locus_tag="NBI57_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020444780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC family protein"
                     /protein_id="HCD1821878.1"
                     /translation="MEVHKKSNAWALFPLLLFVALFLGVGIITGDFTSMPLNVAITIT
                     VIVALLMNRKESFAKKVEVFTKGAGHSNIILMMLIFILAGAFSNTAEKMGGVKSTVNL
                     GLSLIPENLIIVGLFVICMFVSISMGTSVGTVAAIAPVGYGFAQATDVPTALAMATVV
                     GGAMFGDNLSMISDTTIAAVRTQHTKMKDKFRVNFKIVLPGAILTIIILYFLTNGISL
                     NHAKNYDYNLIKVVPYILVLVLALLGVNVIIVLIGGTLLAGIIGLIDGSFGWMGLLDA
                     VSKGIISMEDIAMIALLIGGLVGIIQHNGGIEWLLQFVRSKVKSKRGAELGIASLVSV
                     ADIATANNTISIIMSGPLAKNIADEYDVDSRKSASILDIFGGCFQGLLPYSPQVISAA
                     GVAGISPLMLFPYSIYPILLGVCGLIAILFNFPKLKKNTSQEMKH"
     regulatory      complement(11350..11445)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            complement(11498..12067)
                     /locus_tag="NBI57_000915"
     CDS             complement(11498..12067)
                     /locus_tag="NBI57_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1821879.1"
                     /translation="MALIVSRAILFLILALAFGIATVFPEQKLPLYIVFTLFVAVAII
                     MNVWFTHQNVKKHIIKFNKYVAYFLSFVSGLLYIGFVIYTLTIGSYDVLPIIIFAISI
                     VLVQILENILGISIKNTRLSNKQLKVISKQRVQQFKVISLILVSVSIVFIFIALMLKW
                     WLLLVISIIVLIIITVAMIVYEKYYTTPD"
     gene            complement(12554..13435)
                     /locus_tag="NBI57_000916"
     CDS             complement(12554..13435)
                     /locus_tag="NBI57_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCD1821880.1"
                     /translation="MATQDPRTKFKTTEYEKQEQEVPGLQSEMTPAPDCGETSYQGHQ
                     RLQGYKMLVTGGDSAIGRAAAIAYAKEGADVAINYLPSEEQDAQEVRQVIEESGQKAV
                     LIPGDIRDEQFNYDLVEQAYQQLGGLDNVTLVAGHQQYHDDIHGFTTEAFTETFETNV
                     YPLFWTVQKALEYLKPGASITTTSSVQGYNPSPILHDYAASKAAIISLTKSFSEELGP
                     KGIRVNCVAPGPFWSPLQISGGQPQSKIPTFGQKTPLGRAGQPVELCGTYVLLASEES
                     SYTTGQVFGVSGGVQID"
     gene            complement(13546..14667)
                     /locus_tag="NBI57_000917"
     CDS             complement(13546..14667)
                     /locus_tag="NBI57_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 peptidase aminoacylase family protein"
                     /protein_id="HCD1821881.1"
                     /translation="MGLKDQAVTWRRYFHQFPELSDKEFKTTQKIKDILTEHHIRILD
                     LPLMTGLVAEVGQGLSCIAVRADIDALPIQELVEQDFKSENEGVMHACGHDIHMASIL
                     ATAVKLKEIEGTLTGRVKFIFQPAEELGHGAFKIIETDALKDVQAVLGFHNDPSRSVG
                     TFAIKTGAITSAVDRFEFHIKGVGGHAAKPEQCNDPVIVLAQLINSIQSIVSRNLSAF
                     DEAVVTIGQISCGNTWNVIADHAYVQGTVRSFDPVVRKLVETRLQDIADGLAQAYNMK
                     INLNYTHLPGAVMNDEALTHKAIAVAQHVGYKVEMMEQPLTIGEDFSGYSQHFPSVFA
                     LIGSHSEYDLHHPQYKPDERILEKVPEYFVEFVKRLLHE"
     gene            complement(14943..16181)
                     /locus_tag="NBI57_000918"
     CDS             complement(14943..16181)
                     /locus_tag="NBI57_000918"
                     /EC_number="3.5.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazolonepropionase"
                     /protein_id="HCD1821882.1"
                     /translation="MNDLIINHIAELILPKSTDKPLKGKELDELNVVKNGTVVIKDGK
                     IVYAGQHTDDYDATETIDASGKVVSPALVDAHTHLTFGGSREHEMSLKRQGKSYLEIL
                     EMGGGILSTVNATRETSEDDLFKKAEHDLLTMIKHGVLAVESKSGYGLDRENELKQLK
                     VSNRLAEKYDLDMKHTFLGPHAVPKEASSNEAFLEEMIALLPEVKQYADFADIFCETG
                     VFTIEQSQHYMQKAKEAGFKVKIHADEIDPLGGLELAIDEQAISADHLVASSDKGKEK
                     LRNSDTVAVLLPATTFYLGKEDYADARGMLDNNGAIALATDYNPGSSVTNNLQLVMAI
                     AALKLKLSPSEVWNAVTVNAAKAIDINAGTINTGDKANLVIWDAPNHEYIPYHFGINH
                     AEKVIKDGKVIVDNTLSFKA"
     gene            complement(16181..17842)
                     /gene="hutU"
                     /locus_tag="NBI57_000919"
     CDS             complement(16181..17842)
                     /gene="hutU"
                     /locus_tag="NBI57_000919"
                     /EC_number="4.2.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HCD1821883.1"
                     /translation="MRKIQAKKGLSIECKGWEQEAVLRMLYNNLDPEVAERPEDLVVY
                     GGIGKAARNWEAFEAIEKTLRELESDETMLVQSGKPVAVFKTHEEAPRVLISNSVLVP
                     EWANWDHFNELDKKGLIMYGQMTAGSWIYIGSQGIVQGTYETFAELGNQHFNGDLAGT
                     VTLTAGLGGMGGAQPLAITMNHGVAICVDVDETRVDKRIDTKYCDVKTADLDEALKLA
                     EEAKERGEGLSIGLVGNAVDIHQAILEKGFKIDIITDQTSAHDPLNGYVPQGYSVEEA
                     KVLREKDPKKYVELSQASMAKHVELMLEFQKRGAVAFDYGNNIRQVAFNNGVKNAFDF
                     PGFVPAYIRPLFCEGKGPFRFAALSGDPKDIERADEEMRKLFPENEKLLRWLDLAEEK
                     ISYQGLPSRIAWLGYGERAKMGLALNRLVRDGEISAPIVIGRDHLDAGSVASPNRETE
                     SMKDGSDAVGDWAVLNALINTAAGGSWISFHHGGGVGMGYSLHAGMVVVADGSERAER
                     RLERVLTTDPGMGVARHVDAGYDIAIQTAKEKGIHIPMIDKAGDK"
BASE COUNT         5919 a         3556 c         2492 g         5926 t
ORIGIN      
        1 ttactaagat ttaaatagat gagtacataa tgtataaatt gggaatatac agtatgactt
       61 acggtaatat atcaatatat tgatatatta tttgcacatg ggagtatgct attttttatc
      121 tatacatctt aaaaaaattt acagttttac aattcaatat cattaaacaa acaacatatt
      181 cagcttattt ttcattctct tacgttacaa gttgtaaata ccatcgcgtt aaacaacaat
      241 agccaacgct aataaaatat attgcgttaa agtggagtga ttaatatgtc aaacgtacta
      301 acagaaatag atagtcaata tccatatatg actaaaaatg aaaaaaagat agctaaattt
      361 atactaaatt cacctcaaaa ggtgattaaa atgaggtctc aagacttagc aagcttatta
      421 gatattagta catcatcagt cattcgattc agcaagaaaa ttactgatgg cggttttcac
      481 gacttgaaaa taaatatatc taaatatgtt cctaaagcat catcaattta caatgttgaa
      541 ttaatgaata acgaaagcac agaatcttta agaactaaac tccatacacg tactacacgc
      601 gcacttaatc atgcaaataa cgaattaaat gataaaacta ttgatcaaat atgtcattgt
      661 ttaaaacgtt ctgagacgat ttttatatat ggttttggcg catcttttgt agttgctacc
      721 gacttatacc aaaagttatc aagaataggt cttaatattc aacttgttca agaaacacat
      781 atttttgcta cattattagc aactcacaat tcgaacgaca gtgttattct tattactaac
      841 aatggtacgc aaagtgaaat gcaatcaatg gttaaagtta ttgatgacta ccatatacct
      901 ataattacaa ttactagtac aagggataat cccgtagcac aggcatcaaa tattgtttta
      961 acttatggga aaactgatga aaatgagatg catatgggag caacaacttc attatttgct
     1021 caaatgttca ctattgatat tttatattat cgttatgttg cattaaatta tcacgcatca
     1081 ttagatttca taacacaatc taaaatggct ttagataact atcgtaaaca tttatcaaat
     1141 attaacttta aacattaaca gtcttatata aattctttaa tcatgtcata taataattta
     1201 tgcatcattt tcaaagctta catgtctttt atcttgtata acatacgcat atgaccaatg
     1261 atttgataag gccattcaat ttcttatatt aaaaacaaac gcagttgatt acaggattca
     1321 ttccatatat caattgcgtt tgttatttta ataatgctat tttttatttt aaagcccaca
     1381 gtaaaaacta acgataatat tgccacttat aagtttttca cactacaact tcgatttaag
     1441 ctatatttat aaaaaaatgc gcctactcat aacattcttt ggctatttaa ctttaataag
     1501 ctagggccaa atttgtcata aaataagcgc acattataat catactattt tgcttttaca
     1561 ttatttttat tatatctaat aaagtaataa atcgttacaa tcgttaagaa gatgacacca
     1621 attagcactg atattctagt ttcaacatta aataccatac caaccaatac taatattaag
     1681 aataagattg ttaaatagtt agttactgcg ccaccaggca ttttgaatgg gtgaccttcc
     1741 aattcttcag gatgtaattt tctaaaacgt aagtgactga ataaaatcat aaaccaagga
     1801 atcatacctg gtaaaataga cgcactataa acgtatacga aaatactctt cgctccatcg
     1861 atatataaag gtaaaattac gtttaataaa gcaccaatta aaataccaag agatactgct
     1921 acaacagtgt acaatggtac accatttctc atgattttag taaatatttt tggcatttca
     1981 cctttatgag caagtgtata aatcatacga ctcgcactga aaatacctga gttacatcct
     2041 gacattgctg cagttaatac aacaaagtta attaatccag ctgcaaatgt aataccgatt
     2101 ttagcaaatg ttgcaacaaa cggacttcca atgtctccta attgattcca aggatacact
     2161 gaaacaataa caaaaatagc gcctaaatag aaaattaaaa ttctccagat aacaccattc
     2221 actgctttaa cgatattttt ctgaggatct ttagtttcac ctgccgtaat acctatcagt
     2281 tccacacctt ggtatgaacc aattacaatt gataatgcaa agaagaaacc aacaatacca
     2341 tttggcataa atccgccatt tgtccataga ttagaaatac ctaccgcatg gccaccattt
     2401 ccaaaaccaa agaaaataag accaaaacca gcaataatca ttaaaataat tgttacaact
     2461 ttaattaaag caaaccaaaa ttcaaattct ccaaacgctt ttaccgagaa taaattcgct
     2521 gccattaata ataaaatagc aataacacca ggaatccaag ttggcaattc cgggaaccaa
     2581 aacttcatat attctcctac tgcgatgacc tcactcatac caactacaat ccattggaat
     2641 atattactcc atgctgtcat ataacctgct gcaggatgta tataatcact tgcaaatgtt
     2701 gcaaatgatc ctgttgtagg gtttaaataa atcatttccc ccattgctct catgattaaa
     2761 aataaaaaga tacccgcaat taaatatgca aggataactg atgggcctgt ccatttaatt
     2821 gtacttgtgg cacccatgaa aagccccacg ccaatcgtac caccaagtgc aatcatttga
     2881 atttgacggg cactcaatcc cctttgtagt tcattgttct ccataacttc ttaccccatc
     2941 tctgattgat actaaataaa tccataaaaa aacttattgc cgtaattata ctataaatta
     3001 tcgattttgg tacattaata tttgcatacc atgcaatcgt ttacagaact actatagcag
     3061 gcttttgcaa ctttgcaaac aattattttg tgatttttct aaaaaattaa aaaagttgct
     3121 caagtaccgt tgagtcacat acaacttcag ctattctcgg taattatgat ataattttaa
     3181 taatattaaa aaggagtata tcaatgttaa aacaaatact gccacgtgct acaaaaatat
     3241 cgatactctt tgctgtagca tttttcatca taaattatat cggtatggaa aaacctgaca
     3301 tcctatattt agttggaaga actattattg caacacttgc tttcatactc atttgtctta
     3361 cattatttac tattattaat tctccagagc gcaagattaa actcggtacg actttaccga
     3421 ttgcattaat tattggtatc attttcggcg ccatattttt aacggtacaa atcggtgtca
     3481 tcactgggtt aatcatcggt gtgattgcta cgtttatttg ggaattgatt gaaaaaaata
     3541 aaggaggtcg ttcatcatga caacagcatt aattggtgta attgtattaa tcttactaat
     3601 tattagcttt gtgccaaact accgtgctat gaaatcagca aaagaacaag gaagtaatgc
     3661 aacaagagct acaattatgg taggcataga tattatctta attgtactta tcatcgtgac
     3721 attattatta aaatatatgt aactatttaa accaccctat ctcatacata aacatgaacg
     3781 ttctgccatt tcacatacat atggtcgtcc atttaatgag gtaaggtggt tttctcgtta
     3841 aagtttacct tgtaatagta aaaaatagcc aattgttgcg agtattaagt aaatcattga
     3901 accaacaaca aaagatctta cttgcacgcg aaatggtgtc tgtttgttaa atataggacc
     3961 aatcaccaaa tttccaacgc ctaaaattac tggaccagct agaaaaaaca atataatgta
     4021 acccatcatg atacgcctcc tatttcgtaa agaaacgcgc tacaatttga tcgtaatcaa
     4081 tatcttttgc tacataatct acattagaaa agtcctgtgc gcttgtcatt ttattcgtat
     4141 taacaataac atctaatcca gctgcaatcg cggctgttgc accatttaca gaatcttcaa
     4201 tagccaaaca ttgtgtcggc atataattta attgttgtac tgcagataaa tataattcag
     4261 ggtcaggttt aacttgttca acatcttctc taccaacaac tacctctata taatcgtcta
     4321 gacctaatgc tttaaaggtg ggcattattt ccgctttcac actacttgtg gcaattgcca
     4381 taggaatgtg acgttgcttt aaaaatgcca tcaatgattt aatcgtatca atcatcggta
     4441 atgttgtact agtaagatga tgttcttcat aaagtttatc tttattttcc gaaccaatcg
     4501 ctttaattaa atggtcatgc aaatctgtag ctgctcctcc aatagaagaa cgataaaaat
     4561 caatgcttat aggatcggca ttatgcatct ctaaatgttt attaataaca ttaaataaat
     4621 gttgttccgt atctattatt gttccatcga aatcaaatat aactgctcta tacatatatt
     4681 tactcctcta ttttttctct ttatcaaagc ttaacatagt ccgatgacca cttcattcta
     4741 ttagtgcaat tataactttt ttgaccaata ctttgctaaa ataggccctg ttatattatg
     4801 aatgaaacta aacactgcgc ctggtactgc tgcaattgga ttaaaatgca atgctgctaa
     4861 tgacacagct aaaccagagt tctgcattcc aacttcaata ctgactgctt tttgatcttg
     4921 tcgatctaat tttaaaagac gagctaacca atatccaatc gtatacccta atacgttatg
     4981 taaaataact actaaaaata ttaataatcc tgtagttaag atttggtgct tactgccacc
     5041 tacaactatt gctaaaatta atgaaatagc aacaactgat ataatcggca aagctgtaga
     5101 agctttttca gcaattttac gattaataat ttgcaaaaca atacctaaag caattggaat
     5161 taatacaact tgaacaactg accacaacat actcaggaaa gatacttcca accattcatt
     5221 tgcaaataga tatattaacg caggtgtaac gaatggcgct agcaacgtag agaccgtcgt
     5281 aatagaaaca gaaagtgcta cgttagcttt ggctaaataa ctcattacat tacttgatgt
     5341 cccacccgga caacatccaa ctaatattac gccaacagca atatcaggtg gtaaatgaaa
     5401 agactttgca attataaatg ctaatgtggg cataatactg aattgtagac agacaccaat
     5461 aattactgct ctaggtgctt taaagaccat tttgaaatca ttaggtgtaa ttgttaatcc
     5521 catacctaac ataacaatac caagtaaata aggtacccac ttacctaatg tcgcaacatg
     5581 ttgtgggaaa ataaatccaa taacagctgc tactaacatc cataccaaaa aactcttcgt
     5641 ggcaaatata ctcaattttt gaaacattgt taacacatcc tcgtcgataa tattgaaaaa
     5701 tattaccata agaccagtaa ggttacaatt acttttctga aaattaagat tatttagcca
     5761 ttctcttctt attgcaaata ttttatcact gataaacaat tattttaaac ttcattcaaa
     5821 agtcttagtg aataaagaaa aaaagcgtgg taaaatcaac ttgaacaaaa ttaagtcacc
     5881 tttataaata ttaaacgtgt atttaatgcc atgtattcct cgggaggtcc tcacatgtta
     5941 attgataaat tcgaaacata catcatcaac atcgccggtt taaatgaccg tactactaga
     6001 aaaaaattaa gtaagttatg caaaagtgta caattttgtg atgcgttgca attctcaata
     6061 aacaaacagt ttaatcagta tgtattagaa attagcttac caaagcaaca acttccatac
     6121 ttcatttcat ttttaagttt tcatcagtat agtattttcc aagtactttc accaaagaaa
     6181 ataaatgaat tacttgattc tgataattta tatcaatctg caaagcgttt cgatatcaat
     6241 attgatggct tgcaagatgc tttcattaaa gacaaagtca tcgatattat gaatatgttc
     6301 caaaatcaca ctgatatcac ttatacgttg aataagtctc atgcacatat tatatgtacg
     6361 ccagaaatat ttgctaaatt gttacatacg attgcaacta gaaatatcga cattctatct
     6421 gccaattata gatcgtcatc tatgagcaaa gctcgtatat cataataaaa gacacatcta
     6481 tacgatgatc atttacaatg attgcgtata gtgatgtgtc tttttttgta ttatttatct
     6541 tcggacgatt gatcctcaac catttgttca aatgcttcac gaacttgtgg tactgtcata
     6601 cctactacaa cttggatact cttaccactc tttactaatc cgtgtgccat ttgttgatgc
     6661 gtgaaatatt ccgtatccgc aactttactt tcatcataaa cagttaagcg taatcttgtt
     6721 gtacagttag taacatcttt gatattttct ttgccgccta aaccgtcgag gtaatacgca
     6781 gctttgtctt cgtattcatt acctgttgaa gcacgtttag aatctacgct atcacctttt
     6841 ttatttttgt aatcttgctt agaaaataat ttaacttcct cttctgtttt cttacgtcca
     6901 ggtaatggaa tatcaaattt caaaattaag aatctgaata agaagaagta aatcgctaca
     6961 aatatcagac cgataataac ttgtgcaaca taagtacccc aatgttcttt acctaatggc
     7021 aaccagtttg ttgaaataaa gtctaacaag ccaccaccca tgttacctac aacaccaaat
     7081 gcgtacatta aagtatccat tgatgctgct aaaactgcgt gtaaaacaaa taagtatggt
     7141 gcgatgaata agaatgtaaa ttctaatggc tcagtaatac ctacaacgac tgctgttaat
     7201 gttgcaggaa taagtaatgc tgctactttt ttacgatttt ctttaggtgt cgtagcatac
     7261 attgctaaag caatacctaa acatccaaat actttcccat ttccttgaag cataaagcca
     7321 taagggaatt gctcttttaa tggcttagta ctcttagcaa actcatttaa atgttgtaac
     7381 cattcagctt tcaagccatg attgactact actggtccaa cttcaattgg tgcataaata
     7441 aagtgatgta atcctgttgg tattaataca cgttctaaga agtggtacag ccaaacaccg
     7501 atatagcctg aagcgatgat aaagtgttgc attgaaccaa taccgtgctg aacagttggc
     7561 caaataacac aagtaattgc tgctaatggt aacatcacaa aaaatgaaat tgtaaccaca
     7621 aatgttaaac cttggaatac accaaccatt tctggtaatc gtttactgta gtatctattg
     7681 tgaatccacg taataatagc tgaaataata ataccaccta gaatattcgt atccaatgtg
     7741 gcaatacctg caattgattt taaaccaggt acattttcaa cgcctttttc taaattagcg
     7801 ccaaatgtat gtggccattg cgttaaaatg gcatttataa atgtattaaa cattaagtaa
     7861 cccatcaatg ctgcaagcgc tgcatgacct ggtgcttttt tagctaaaga aagtggtaaa
     7921 cctacaacaa atacaacttc catatgcgta aaaataaccc agccaccaga ttctatcact
     7981 gaccaaaatt taaaccaaaa cgtatgttga tctgctaaac tccccatgat tgtaggattt
     8041 ttaaataatg tcgcaaagcc aagcactatc ccaaagaaag cgaacattaa taccggtaca
     8101 atcattgcac taccgaaacg ctttatcgca ttcatcttct attccctcca tatcatcttt
     8161 cctaacaata catctaatta gattcattta taaatagatg tcttactatt taaatataat
     8221 atatagtaaa cgcttacaca cctacaacga cattgacgta ttttgaaagt attttgtata
     8281 atcagattat cttttcatat agtgaaaatt ttttcacgac cttatatatg acatcgttgt
     8341 atttgtaata cattcgtttt aaacgcataa tcaaacctat atcaatacac aaatatatat
     8401 aatgacatac aagattttaa tgtaataacg atctatcaca catttatttt caaggaggtt
     8461 gaatatgttt ttagatgaac acattaatcg aaactttgat aaacttaatg ataatgattt
     8521 acatatcgct cactttatca atacacatat agatgaatgt aaaaatatga aaatacaaga
     8581 tttagcgcaa ttcacacatg cctcgaatgc aaccattcac agatttacac gcaaattagg
     8641 ttttgacggt tatagtgatt ttaaatcgta ccttaaattt gaaagtaata agacacacca
     8701 attaccttct aactctatcg atagtttcaa acaagaaatc gatagtacat tcagttattt
     8761 agaacgcgtt gattaccagc ttcttactga aaaaatgcat acagcttcaa caatatattt
     8821 gtatggtaca gggcgtgcac agttaaacgt tgcttcagag gcacagcgta tattgttaac
     8881 gttacataat catgtcattg tacttcatga tgaacatgaa cttaaaatga ttttgaacaa
     8941 gagtgaacct aatgatttat tctttattat ttcattatca ggtgaaacac atgaattatt
     9001 agaagtaaca aacttgcttc aacttagaca aaaatacttt atttctgtta ctacaatgaa
     9061 agataatacc cttgctcaaa aagcaaatta caacgtctat gtatccagta acacattcta
     9121 cttaactgat ggtacagact attctagctt catcagttat catatattct ttgaaacact
     9181 tgtaagaaaa tataatgagt atttactaca taatgatatt tcaaattaga aaaaaggaag
     9241 ttatgattta atggccttga gcctatcata acttcctttt atcattttat tgttgtgttg
     9301 atgtttcgat aacgtggtac atcttatcaa acatcaattc gaaaccatgc accatggcat
     9361 catgatattc ttttttcttt tgcttgtatt ctaaattagt aaatcgtctt tctttttcaa
     9421 ctaatgaacg ataataaaat agcatttggg tgccacctgt ttcacgttca aaaaattcta
     9481 cctcaatgac atcttgtgtt tcacttagtc caggcatacc gatagtcatc ttaacgtatt
     9541 catccataac taaagattca taaatgcctt caatcacatt tactttgcca ttacgttgtt
     9601 gatctacaat acgatattta ccgccttctt taacgtccgc ttcaatctct ttattcgttc
     9661 tggctgatgt cataaaccat tgtttcaaca aatctttctt tgtccaagct tcgtatacta
     9721 actctggaga aaatttataa agcttttcaa tttcaacttc gacatgttca ttctctacat
     9781 taaattttgc cactgttgtc cacccacttt cgctcttact tttattttaa cgtatttttg
     9841 ctcagttcca aacatagatg atcatcattt ttaaaagatt agcgttatac ggtgagtaca
     9901 acatgatctg ttaatataac aagccacctt acttggctac atcgatatat tgttaagcat
     9961 taatgtttca tttcttgact agtgttcttt tttagctttg gaaaattaaa taaaatcgca
    10021 ataagtccgc atacacctaa taatatagga taaatgctgt atgggaataa cattaatggt
    10081 gaaataccag ctacaccagc cgctgaaatg acttgcgggc tatatggtaa taaaccttgg
    10141 aagcagcctc caaatatatc aagaatactt gctgatttcc ttgaatctac atcatattca
    10201 tctgcaatat ttttagctaa aggacctgac ataataatag agatggtgtt gtttgccgtg
    10261 gcaatatctg cgacacttac caaactcgca attcctaatt ctgcgccacg ctttgatttc
    10321 actttagagc gaacaaattg caacaaccat tcaataccac cattgtgttg aataataccg
    10381 actaaaccac caattagcaa cgcaatcata gcaatatctt ccatgcttat aatacctttg
    10441 gacactgcat ctagtagtcc catccaaccg aatgaaccat ctatgagacc aatgattcca
    10501 gctaataatg ttccgccaat caatacgata atgacattta cacctaataa tgctaatacc
    10561 aatactaaaa tatacggtac aactttaatt agattataat catagttttt agcatgattt
    10621 aaagaaatgc cattcgttaa gaaatacaga ataataatcg ttaaaatagc acctggcaat
    10681 acaattttaa agtttactct gaatttatct ttcattttcg tatgttgtgt tctaaccgca
    10741 gcaattgttg tatctgaaat cattgataga ttatcgccga acattgcacc tccaacaact
    10801 gtagccattg ctagcgcagt tggtacatct gtcgcttgtg caaatccgta accaacaggt
    10861 gctattgcag caacagttcc tacagacgtc cccatagata tagaaacaaa catacaaatc
    10921 acaaacaatc ctacaataat taaattttct gggattaatg atagtcctaa attaactgtc
    10981 gactttacgc cacccatttt ttcagctgta tttgaaaatg cacctgctaa aataaaaatc
    11041 aacatcatta aaataatgtt tgaatggcct gcacctttcg tgaagacctc aactttttta
    11101 gcaaatgatt cttttcgatt cattaataac gccacaatta ccgttatcgt aattgcaaca
    11161 tttaatggca ttgaagtaaa atcacctgtg ataataccta cgcctaaaaa caacgccaca
    11221 aataataaca aggggaataa tgcccaagca ttgctctttt tatgtacttc catccttttt
    11281 acctgctttc caattaaaaa tacctctttc tcacaaacga tgaagaaaga ggttttcatg
    11341 tgctttacct gcttatcttc aaaccattac ggttactgga attggcacat tcgagatgtt
    11401 gccgaggctt catagggcca gtccctccac ctctctagat aagtgatgct tatttacgtt
    11461 tacgttacaa gataatcctt agtacgtcaa tcataaatta atcaggagtc gtataatatt
    11521 tttcataaac aatcattgct actgtaataa taatcaaaac aataatgcta ataacaagta
    11581 aaagccacca tttaagcatt aatgcaataa aaatgaacac gatagacaca cttactaata
    11641 ttaatgatat gactttaaat tgctgaacac gttgcttgga gatgactttc aactgtttgt
    11701 ttgatagacg cgtatttttt atactgattc ccagtatatt ttctaatatt tgaaccaata
    11761 cgatacttat tgcaaatata ataattggta aaacatcata gctccctata gttaatgtat
    11821 aaattacaaa tccaatgtaa agtaaccctg agacaaagga taaaaagtat gcgacgtatt
    11881 tgttaaactt aatgatatgc tttttaacgt tttgatgggt aaaccataca ttcataataa
    11941 tcgcaactgc tacaaataat gtgaatacta tatataatgg taatttttgt tcaggaaaaa
    12001 cagtcgctat tccaaaagct aatgctaaaa tcaaaaataa tatagctcta gatactatta
    12061 atgccataat aacaacccct ttgtttaata tcgagtttgc aaatttacgt ttatcagcat
    12121 ttctatgatc agtacttcta cgggtagcgt ttctatgtaa tttacatcat cttaacatat
    12181 aaatacttcg ctatttaatt gaaaacatat cttattattc tttgtccgtt ctgacattta
    12241 atatctaacc ttaggcattt cacttgttaa taaatttaac tttcttccac taaccgtccc
    12301 taaacccaat cccgcaacag tttttaactt tttcgttgtt gtcctgacat cctcattaag
    12361 aaagtttatt ctgcttaaaa cttataatcc acaccctgag caaacgctcc ttatgacaga
    12421 gtattaaaat aagccgataa agatacacac ctttaccgac tatttaaaat acacttcacc
    12481 aattcatttt aatttaatgg attgaagtaa ctaaattaat attatgctgt tcaattaaaa
    12541 gcttcataca accctaatct atttgcactc caccgctaac accgaacact tgtccagttg
    12601 tataacttga ttcctctgac gctaatagca cataagttcc acataactca acaggttgac
    12661 ctgcacgacc taaaggtgtt ttttgaccaa atgttgggat tttactttga ggttgcccac
    12721 cagaaatttg taatggtgac cagaatggac caggtgctac acagttcact ctaattcctt
    12781 ttggtcctaa ttcttctgaa aaacttttag ttaatgaaat aattgctgct tttgaagcgg
    12841 cataatcatg aagaatagga ctaggattat aaccttgtac agatgatgtc gttgtaattg
    12901 acgcacccgg ttttaaatat tccaatgctt tttgaactgt ccaaaatagc ggatagacat
    12961 tcgtttcaaa tgtttctgta aatgcctcag ttgtaaatcc atgaatatca tcatgatact
    13021 gttgatgtcc agcaactaaa gtaacattat ctaagccacc taattgttga tatgcttgtt
    13081 caacaaggtc atagttgaac tgctcatctc ttatatcacc aggaattaac actgcctttt
    13141 gaccactttc ttcaattact tggcgtactt cttgtgcatc ttgttcttca ctcggaagat
    13201 agttaatcgc tacatctgca ccttctttag catacgcaat tgctgctgca cgccctattg
    13261 ctgagtcacc acctgtgact aacattttat agccttgtaa gcgttgatga ccttggtaag
    13321 acgtttcgcc acaatcaggt gctggcgtca tttcagattg taaacccggt acctcttgtt
    13381 cttgtttttc atattccgtt gttttaaatt ttgttctagg atcttgagtt gccatttttt
    13441 aacatctcct tattcgctta atggttatta tttacccaat cttcctagga acttaatcat
    13501 gattacacta aaaattactt tcttctttat aaaaacaagc tcgaattact catgcaatag
    13561 tctctttaca aattcaacaa aatactcagg tactttttcc agaatccttt catccggttt
    13621 atattgaggg tgatgtaaat catattcact atgagaacca attaacgcaa atacacttgg
    13681 aaaatgttga ctataacctg aaaaatcttc tccaatcgta agcggctgtt ccatcatttc
    13741 caccttatat ccaacatgtt gggctactgc aattgcttta tgcgtcaatg cctcatcatt
    13801 catcacagcg ccaggtaaat gtgtataatt taaattaatt ttcatattat atgcttgagc
    13861 caatccgtcc gcaatatctt gtaatcgtgt ttctacaagc tttcgtacca caggatcaaa
    13921 actacgcact gtgccttgta catacgcatg atcagcaatg acattccaag tattaccaca
    13981 tgatatttgt ccaattgtta ctaccgcttc atcaaacgca gatagatttc tactaactat
    14041 ggattgaata ctattaatca attgcgccaa cacaataact ggatcgttgc attgttctgg
    14101 ctttgcagca tgaccaccca cgcctttaat atgaaactca aagcgatcta ctgctgatgt
    14161 aattgcccct gttttgattg caaatgtacc taccgaacgc gatgggtcat tatgaaaacc
    14221 caatactgct tgtacatctt ttaatgcatc tgtttcaata attttaaaag cgccatgtcc
    14281 tagttcttct gctggttgaa aaatgaattt aacacgccca gtaagagtgc cctcaatttc
    14341 ttttaatttt acagccgtag ccaaaatact agccatgtga atatcatgac cgcacgcatg
    14401 cataacacct tcattttcag atttaaaatc ctgttcaaca agctcttgaa tcggtaaagc
    14461 atcaatgtca gcccgaacag ctatacaact cagaccttgt cccacttcag caaccagccc
    14521 agtcataagc ggtaagtcta atattctaat atgatgttct gttaaaatat ctttaatttt
    14581 ttgtgtagtc ttaaattctt tatcggatag ttctggaaat tgatgaaaat accttctcca
    14641 ggtaacagct tgatctttta atcccatcgg tcattcccct tccttaagtc aatgatatgt
    14701 tgtctaccct acgatgatca tctttgacta ttaaacgatg atttcacaac aatgtactct
    14761 tgttaattac tttcgctaat gatagacagt tgtttaataa tatcgtaaca ctgttgtcaa
    14821 actattctaa cttttataat taagaatcta tacaaaaacg tgttctcgga taaacttgtt
    14881 tttacaaacc acaaaagctc taaacattag tgtaaaccaa tgcttagagc tttctaatta
    14941 ttttatgctt taaaagataa tgtgttatct acgatgacct taccgtcttt aataactttt
    15001 tctgcgtgat tgataccaaa atgatatgga atatattcat gatttggtgc atcccaaatt
    15061 actaaattag ccttatcacc tgtgttaatt gtacccgcgt taatatctat tgctttagca
    15121 gcgttgaccg taacagcatt ccacacttca ctaggcgaaa gctttaattt taatgctgca
    15181 atggccataa caagttgcaa gttattcgtt acactgctgc ctggattata atcagtagct
    15241 aacgcgatgg caccattatt atcaagcata cctctcgcat ctgcataatc ttctttacct
    15301 aaatagaacg tcgttgcagg taagaggaca gctacagtat cactatttcg caacttttct
    15361 tttcctttat cactagaagc tactaagtgg tctgctgata ttgcttgttc atcaattgct
    15421 aattccagtc cgcctaacgg atcaatttca tccgcatgta ttttcacttt aaaacctgct
    15481 tctttggctt tttgcatata atgttgcgat tgttctattg taaatacacc tgtttcacag
    15541 aaaatatccg caaagtctgc atattgtttt acttccggaa gtaacgcaat catttcttct
    15601 aaaaatgcct catttgaact tgcctcttta ggtacagcat gaggccctag gaaagtatgt
    15661 ttcatgtcta aatcatattt ctcagctaaa cgattagaca ctttcaattg cttcagttca
    15721 ttttctctat ctaatccata accactctta ctttcaactg caagcacgcc gtgtttaatc
    15781 atagtaagca aatcatgctc tgctttttta aacaagtcat cttcggatgt ttctctagta
    15841 gcattaacgg tagataatat gccaccaccc atttctaata tttcaaggta agacttacct
    15901 tgacgtttta atgacatctc atgttctcga gatccaccaa atgttaaatg ggtatgagca
    15961 tctactaatg ccggagaaac tacttttcca ctagcatcta tcgtctcagt cgcatcatag
    16021 tcatctgtat gttgtccagc atatacaatt ttgccatctt taatgacaac tgtaccattt
    16081 ttcacaacat ttaattcatc taattcctta cccttcaaag gtttatctgt tgatttcggt
    16141 aaaattaatt ctgctatgtg attaattatt aaatcattca ttatttatca cctgctttat
    16201 caatcattgg aatatgaata cctttttctt tagctgtttg aatagcgatg tcatagccag
    16261 catcaacatg tcgggcaaca cccatacctg gatcagtcgt caatacacgt tccaatcttc
    16321 tttcagcacg ctctgatcca tctgctacaa caaccatacc cgcatgaagt gaatatccca
    16381 tgccaacacc gccaccgtga tggaatgaaa tccatgaacc acctgcagct gtgttaatga
    16441 gtgcattcaa tacagcccaa tcaccaaccg cgtcactgcc atctttcata ctttctgttt
    16501 cacggttagg actagcaact gaaccagcat ctaaatggtc tcgtccaata acaattggtg
    16561 ctgaaatttc accgtcacgt acaagacgat ttaaagctaa gcccattttc gctctttctc
    16621 catagcctaa ccaagcaata cgtgatggta gtccttgata tgaaattttt tcttcagcta
    16681 aatcaagcca tcttaataac ttttcatttt ctgggaaaag tttgcgcatt tcttcatccg
    16741 cacgctcgat atcttttgga tcaccactca acgcagcaaa gcggaatggc cctttacctt
    16801 cacagaataa tggtctaatg taagctggta caaagcctgg gaagtcaaaa gcatttttca
    16861 ctccgttatt gaaggctact tgacgaatat tgttaccata atcaaatgct acagcgccac
    16921 gtttttggaa ttcaagcatt aattcaacat gctttgccat tgaagcttgt gacagttcaa
    16981 catatttttt cggatctttt tcacgcaata ctttcgcttc ttctacagag tatccttgtg
    17041 gcacatatcc atttagcgga tcatgtgcac ttgtttggtc agtaataatg tcaattttaa
    17101 atcctttttc tagaatcgct tgatggatgt ctacagcatt tccaactaac ccgattgata
    17161 atccttctcc acgttctttc gcctcttctg ctaattttaa tgcttcatct aaatcagctg
    17221 ttttaacatc acagtatttc gtatcaattc gcttatcaac acgtgtttca tcaacatcca
    17281 cgcaaattgc taccccatga ttcatagtaa ttgctaacgg ttgcgcacca cccataccac
    17341 ctaaacctgc tgtcagtgta acagtgcctg ctaaatctcc attaaagtgt tgattaccta
    17401 gctcggcaaa tgtctcataa gtaccttgca caataccttg agaaccaata tatatccaac
    17461 taccggctgt catctgtcca tacatgatta aacctttttt atctaattca ttaaaatgat
    17521 cccagtttgc ccattcaggc actaatactg aatttgaaat taatacacgt ggcgcttctt
    17581 catgtgtttt aaatacagca actggctttc ctgattgtac taacattgtc tcatctgatt
    17641 ctaattctcg taacgttttc tctattgctt caaaagcttc ccaattacgt gctgcttttc
    17701 caataccacc ataaacaact aaatcttctg gtctttcagc aacttctggg tctaaattgt
    17761 tgtataacat tctaagtact gcttcttgtt cccaaccttt acactcaata ctcaaacctt
    17821 tttttgcttg aatttttctc ataaaattcg ctcctgttct tttaagaagt taattccact
    17881 aaatttaaaa cgc
//
LOCUS       DAHHSC010000035        17800 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.035, whole genome shotgun sequence.
ACCESSION   DAHHSC010000035 DAHHSC010000000
VERSION     DAHHSC010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 17800)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17800)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17800
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.035"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            complement(<1..927)
                     /locus_tag="NBI57_000920"
     CDS             complement(<1..927)
                     /locus_tag="NBI57_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1821884.1"
                     /translation="MSDIIQFPNSSKKLYKDIKRAEQDQNYDLMYEYIVQYERQFELT
                     EEIAMMKCRMLYETGSFLELREETIVLLKTGIQQYDALMIYYVKSLIGLGQYFEAVEV
                     IHQIIDEVKDHKTRMALHPLKEFAKSKLIEDEKRLTQSLTDFDTLSMREQTHLILKLI
                     DNGHFQFQETVLYILKSNTYSYNLISLMIEYLRFANCTQELTIEKYGMDVTFVPANLN
                     GLEHTTLKEKVIPNVMETLNDGALHIAEEAHHIMNNHSIMIYPIDIETLFETNKWINA
                     YECYFKNMLGLKCELQSIDAFNFIQQLDLNNNS"
     gene            complement(946..1554)
                     /locus_tag="NBI57_000921"
     CDS             complement(946..1554)
                     /locus_tag="NBI57_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HCD1821885.1"
                     /translation="MSKFDEQIIVVPREIIFNNEKNTFNGFLNKNKPEGQNIFDALSQ
                     YEVKRRGDMEEDPSYKQLISYCLLENEHGEILVYERLSGGGEARLHGQSSIGVGGHMN
                     DVPGAESINEVLRVNAQRELEEEVGLSEQDSQNMEYIGFINDDNNEVGKVHIGVVFKI
                     TVSTNDVEAKETDTLRIKWVEKGNIESYDDFETWSALILQDL"
     gene            complement(1696..2052)
                     /gene="rplT"
                     /locus_tag="NBI57_000922"
     CDS             complement(1696..2052)
                     /gene="rplT"
                     /locus_tag="NBI57_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HCD1821886.1"
                     /translation="MPRVKGGTVTRARRKKTIKLAKGYFGSKHTLYKVAKQQVMKSGQ
                     YAFRDRRQRKRDFRKLWITRINAAARQHEMSYSRLMNGLKKAGIDINRKMLSEIAISD
                     EKAFAQLVTKAKDALK"
     gene            complement(2099..2299)
                     /gene="rpmI"
                     /locus_tag="NBI57_000923"
     CDS             complement(2099..2299)
                     /gene="rpmI"
                     /locus_tag="NBI57_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001830767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HCD1821887.1"
                     /translation="MPKMKTHRGAAKRVKRTASGQLKRSRAFTSHLFANKSTKQKRQL
                     RKARLVSKSDMKRVKQLLAYKK"
     gene            complement(2328..2855)
                     /gene="infC"
                     /locus_tag="NBI57_000924"
     CDS             complement(2328..2855)
                     /gene="infC"
                     /locus_tag="NBI57_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HCD1821888.1"
                     /translation="MSTIAKDQTQINDKIRAKELRLIGQDGEQIGVKSKREALEMAER
                     VDLDLVVVAPNAKPPVARIMDYGKFKFEQQKKEKEMKKKQKIINVKEIRLSPTIEEHD
                     FQTKLKNGRKFLTKGDKCKVSIRFRGRAITHKEIGQRVLEKYADECKDIATVEQKPKM
                     DGRQMFIMLAPTAEK"
     misc_feature    complement(2884..3009)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00558"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L20 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00558"
     gene            complement(3084..4577)
                     /locus_tag="NBI57_000925"
     CDS             complement(3084..4577)
                     /locus_tag="NBI57_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCD1821889.1"
                     /translation="MSKVQNESNNVVKRGLKDRHISMIAIGGCIGTGLFVTSGGAIHD
                     AGALGALIGYAIIGIMVFFLMTSLGEMATYLPVSGSFSTYATRFVDPSLGFALGWNYW
                     FNWVVTVAADITIAAQVIQYWTPLQGIPAWAWSALFLVIIFSLNSLSVRVYGESEYWL
                     ALIKVITVIVFIAIGLLTIVGIMGGHVVGFEIFNKGEGPILGGNLGGSLLSILGVFLI
                     AGFSFQGTELIGITAGESENPERAVPKAIKQVFWRILLFYILAIFVIGMLIPYDSSAL
                     MGGSDNVATSPFTLVFKNAGFAFAASFMNAVILTSVLSAGNSGMYASTRMLYSMSKDK
                     LAFEAFGKTNKNGVPYMSLLVTAIIVVIIFVLQSLTKGAYEYIVAASGLTGFIAWVGI
                     AVSHYRFRRAFDKQNYDKSKLKYTAKLFPFGPLFAGFLCIVVIIGQDVDFIKTGDFNF
                     SRFLITYMGIPVFLAFFIYHKLRYKTKKIPLEQVDLRQDVSMDEIKG"
     gene            complement(5003..6940)
                     /gene="thrS"
                     /locus_tag="NBI57_000926"
     CDS             complement(5003..6940)
                     /gene="thrS"
                     /locus_tag="NBI57_000926"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HCD1821890.1"
                     /translation="MEQINIQFPDGNKKAFDKGTTTEDIAQSISPGLRKKAVAGKFNG
                     QLVDLTKPLETDGSIEIVTPGSEEALEVLRHSTAHLMAHAIKRLYGNVKFGVGPVIEG
                     GFYYDFDIDQNISSDDFEQIEKTMKQIVNENMKIERKVVSRDEAKELFSNDEYKLELI
                     DAIPEDENVTLYSQGDFTDLCRGVHVPSTAKIKEFKLLSTAGAYWRGDSNNKMLQRIY
                     GTAFFDKKELKAHLQMLEERKERDHRKIGKELELFTNSQLVGAGLPLWLPNGATIRRE
                     IERYIVDKEVSMGYDHVYTPVLANVDLYKTSGHWDHYQEDMFPPMQLDETESMVLRPM
                     NCPHHMMIYANKPHSYRELPIRIAELGTMHRYEASGAVSGLQRVRGMTLNDSHIFVRP
                     DQIKEEFKRVVNMIIDVYKDFGFEDYSFRLSYRDPEDKEKYFDDDDMWNKAENMLKEA
                     ADELGLSYEEAIGEAAFYGPKLDVQVKTAMGKEETLSTAQLDFLLPERFDLTYIGQDG
                     EHHRPVVIHRGVVSTMERFVAFLTEETKGAFPTWLAPKQVQIIPVNVDLHYDYARQLQ
                     DELKSQGVRVSIDDRNEKMGYKIREAQMQKIPYQIVVGDKEVENNQVNVRQYGSQDQE
                     TVEKDEFIWNLVDEIRLKKHR"
     gene            complement(7138..7260)
                     /locus_tag="NBI57_000927"
     CDS             complement(7138..7260)
                     /locus_tag="NBI57_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1821891.1"
                     /translation="MFDENMVNKLDDYYFIYGICNEYPDQDRYLKQRYLIHKLY"
     gene            complement(7353..8273)
                     /gene="dnaI"
                     /locus_tag="NBI57_000928"
     CDS             complement(7353..8273)
                     /gene="dnaI"
                     /locus_tag="NBI57_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HCD1821892.1"
                     /translation="MKQFKSIINTSQDFEKRIEKIKKEVINDPDVKQFLEAHRAELTN
                     AMIDEDLNVLQEYKDQQKHYDGHKFADCPNFVKGHVPELYVDNNRIKIRYLQCPCKIK
                     YDEERFEAELITSHHMQRDTLNAKLKDIYMNHRDRLDVAMAADDICTAITNGEQVKGL
                     YLYGPFGTGKSFILGAIANQLKSKKVRSTIIYLPEFIRTLKGGFKDGSFEKKLHRVRE
                     ANILMLDDIGAEEVTPWVRDEVIGPLLHYRMVHELPTFFSSNFDYSELEHHLAMTRDG
                     EEKTKAARIIERVKSLSTPYFLSGENFRNN"
     gene            complement(8273..9673)
                     /locus_tag="NBI57_000929"
     CDS             complement(8273..9673)
                     /locus_tag="NBI57_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation and membrane attachment
                     family protein"
                     /protein_id="HCD1821893.1"
                     /translation="MGRQAFEFGLRPKDQFKVMQHFDLNTNHLEVLNRLYTPLIGTQA
                     VGLYHFMTQFVKDSHNETLILSHYIFMNELKINLLEFRQQMDLLEAIGLLKAFVKHDE
                     QETQFVYQLIQPPSAHLFFNDPMLSIFLYSEVEHRRFHELKKYFEYQQIDLSEFKQVT
                     RQFTDVFKVPSTKIDIDTSDIPINEPYQGIDLSNESFDFEMLRQMLGKHFISQDIVTK
                     DAKRLITQLATLYGLTADGMKHVILNSITSGQQLSFEEMRKQARSYYLMEHENQMPKL
                     QVKSPATSSSTGKSSEVNPKPQSDEWFELLEQTSPIDMLASWSESEPTISQKTMVEEL
                     IEREKMSFGVINILLQFVMLKEDMKLPKAYILEIASNWKKKGIKTAKEAYNYAKKVNQ
                     PKNEGSSGNYQKRGSYYGQRNRISKEKTPKWLENRDKPSEEDSAKDNSVDDQQLEQDR
                     QAFLDKLSKKWEEDSR"
     gene            complement(9674..10144)
                     /gene="nrdR"
                     /locus_tag="NBI57_000930"
     CDS             complement(9674..10144)
                     /gene="nrdR"
                     /locus_tag="NBI57_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HCD1821894.1"
                     /translation="MKCPKCNSTQSKVVDSRHADELNAIRRRRECENCGTRFTTFEHI
                     EVSQLIVVKKDGTREQFSREKILNGLVRSCEKRPVRYQQLEDITNKVEWQLRDEGHTE
                     VSSRDIGEHVMNLLMHVDQVSYVRFASVYKEFKDVDQLLASMQGILSENKRSDA"
     gene            complement(10355..11380)
                     /gene="gap"
                     /locus_tag="NBI57_000931"
     CDS             complement(10355..11380)
                     /gene="gap"
                     /locus_tag="NBI57_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HCD1821895.1"
                     /translation="MSTNIAINGMGRIGRMVLRIALQNKNLNVVAINASYPPETIAHL
                     INYDTTHGKYNLKVEPIENGLQVGDHKIKLVADRNPENLPWKELDIDIAIDATGKFNH
                     GDKAIAHIKAGAKKVLLTGPSKGGHVQMVVKGVNDNQLDIEAFDIFSNASCTTNCIGP
                     VAKVLNNQFGIVNGLMTTVHAITNDQKNIDNPHKDLRRARSCNESIIPTSTGAAKALK
                     EVLPELEGKLHGMALRVPTKNVSLVDLVVDLEKEVTAEEVNQAFENAGLEGIIEVEHQ
                     PLVSVDFNTNPNSAIIDAKSTMVMSGNKVKVIAWYDNEWGYSNRVVDVAEQIGALLTS
                     KETVSAS"
     gene            complement(11550..12173)
                     /locus_tag="NBI57_000932"
     CDS             complement(11550..12173)
                     /locus_tag="NBI57_000932"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HCD1821896.1"
                     /translation="MPKVIGLTGGIASGKSTVSELLSVFGFKVVDADKAAREAVKKGS
                     KGLAQVREVFGDEAIDENGEMNRRYMGDLVFNHPEKRLELNAIIHPIVRDIMEEEKQE
                     YLKQGYNVIMDIPLLFENELENTVDEVWVVYTSESIQMDRLMQRNNLSLEDAKARVYS
                     QISIDKKSRMADHVIDNLGDKLELKQNLERLLEEEGYIEKPNYGEED"
     gene            complement(12189..13061)
                     /gene="mutM"
                     /locus_tag="NBI57_000933"
     CDS             complement(12189..13061)
                     /gene="mutM"
                     /locus_tag="NBI57_000933"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HCD1821897.1"
                     /translation="MPELPEVEHVKRGIEPYVINQKIEHVIFSDKVIEGKAQGKETII
                     KGIELDTFKTLSEGYTITNVERRSKYIVFQLDNKREQRTLISHLGMAGGFFIVDELED
                     IMIPNYRKHWHVIFELSNDKKLIYSDIRRFGEIRNVASVASYPSFLEIAPEPFTNEAL
                     TYYLNRIHQQSNKNKPIKQVILDHKVIAGCGNIYACEALFRAGVLPDKKVKDLTHQQQ
                     EMVFYYVREVLEEGIKHGGTSISDYRHADGKTGEMQLHLNVYKQPVCKVCGSQIETKI
                     IATRNSHYCPVCQK"
     gene            complement(13077..15707)
                     /gene="polA"
                     /locus_tag="NBI57_000934"
     CDS             complement(13077..15707)
                     /gene="polA"
                     /locus_tag="NBI57_000934"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HCD1821898.1"
                     /translation="MNKLVLIDGNSLSFRAFYALPLLSNKAGIHTNAVYGFAMLLEKI
                     LKEEKPNHFLVAFDAGKTTFRHEKYSEYKGGRQKTPPELSEQFPYIRQLLDAYHIKRY
                     ELDNYEADDIIGTLSKEADKAGFQTIIITGDRDLTQLATDNVTIYYTKKGVTDVDHYT
                     PDFIAEKYNGLTPNQIIDMKGLMGDTSDNIPGVAGVGEKTAIKLLNQFDTVEGVYEHL
                     DEISGKKLKEKLQNSKEDALMSKELATINVDSPIEVKLEDTLMTHQDEQQEKIELFKK
                     LEFKQLLSDIDQSASVEDAIEKTFEIETSFDNIDFTSLKEAAIHFELDGGNYLRNNIL
                     KFSLFTGEKHIVINADDINNYAELVSWLENPNTKKVVYDAKKTYVASHRLGIDIQNIS
                     FDIMLASYIIDPSRTISDVQSVVSLYGQSFVKDDVSIYGKGKKFKVPEDDVLNPYVAS
                     ITDAIYFAKPNMDKQLEEYNQVELLADLELPLAKILSEMEEIGIFTDVHDLEEMEKEI
                     QEKLDVLIRNIHDAAGEDFNINSPKQLGVVLFETLQLPVIKKTKTGYSTAVDVLEQLQ
                     GEHPIIDYILEYRQLSKLQSTYVEGLQKVISDDQRIHTRFNQTLAQTGRLSSVDPNLQ
                     NIPVRLEEGRKIRKAFKPTSKDSVILSADYSQIELRVLAHITQDESMKEAFINGDDIH
                     TATAMKVFGVEADQVDSLMRRQAKAVNFGIVYGISDYGLSQSLGITRKKAKAFIDDYL
                     ASFPGVKQYMSDIVKDAKALGYVETLLHRRRYIPDITSRNFNLRGFAERTAMNTPIQG
                     SAADIIKLAMVKFAQKMKETTYQAKLLLQVHDELIFEVPKSEVDSFSEFVEEIMENAL
                     QLDVPLKVDSSYGATWYDAK"
     gene            complement(16001..17488)
                     /locus_tag="NBI57_000935"
     CDS             complement(16001..17488)
                     /locus_tag="NBI57_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1821899.1"
                     /translation="MKTIQLHKTTLILIAILLFYTFMGILLPIMHDDLQWFSNYNTDI
                     LKVGFASLNGRYIGNIFEIIAVHVSWLRWLSYGLISMGIIWMIMHITRCKAWTSYYLL
                     AFSLMLILPSAIYADTYGWFAGFYNYATSTLISLFIIYYCINAIIYKEKQPVSVTVLF
                     YVLCFFGQLFMENVTLFHCIILILAFLYEFTANRTLNYKLLFSFMIATIGTIIMFSNP
                     NYRKILFEGSEYQQVSNNQGIFSKFAEMISTSLPYGVIFSQIIILSMIAALIIYLLLR
                     SDRYVHLTILKRRIIMIGFITLPLYYLLFYNQFLLNKNTDIGLVGFVNVIVCGYFAFS
                     IFVGIYLSINDRKTQITLYSLLIAICVSASTLVIVTPIEPGNFLIVYTIHVIILIILL
                     KEFRKYKSINVNFIKGASLVLAVIYLSAFIYVHYEHEKRVQLLKQQIKDHPRQEVYTV
                     ESLPFEHYMHHPSPINKNYQEWFNNYEELPKMTKVKYIPFGSDDS"
BASE COUNT         5583 a         3358 c         2395 g         6464 t
ORIGIN      
        1 actattatta ttcaaatcta attgctgaat aaaattaaat gcatcgatac tttgtaattc
       61 acatttaagt cctaacatat ttttgaaata acattcgtat gcattaatcc atttattcgt
      121 ttcaaacaaa gtttcaatat caattggata aatcatgata gagtgattat tcataatatg
      181 gtgtgcttct tcagcgatat gtaatgcacc atcatttaat gtttccataa cgttaggtat
      241 aactttttct ttcagtgttg tatgttctag cccatttaaa ttagctggta caaaagttac
      301 atccatacca tacttttcaa ttgtcagttc ttgtgtacaa tttgcaaacc ttaaatactc
      361 aatcattaaa ctaatgaggt tataactgta cgtattagat tttaatatat ataataccgt
      421 ttcttgaaat tgaaaatgac cattgtctat taacttcaaa atcaagtgcg tctgttccct
      481 cattgataac gtatcaaaat cagttaatga ctgagtcaat cgtttttcat cttcaattaa
      541 ttttgactta gcaaattctt taagaggatg taatgccatt cttgttttgt gatctttgac
      601 ttcatcaata atttgatgaa ttacttctac cgcttcaaaa tattgtccca aaccaatcaa
      661 acttttcacg taataaatca tcaatgcatc atattgttgt atgccagttt tcaataagac
      721 aattgtttct tctcttaatt caagaaatga ccccgtctca tataacatac ggcatttcat
      781 cattgctatt tcttcagtga gttcaaattg acgttcatat tgcacaatat attcatacat
      841 caggtcatag ttttggtctt gttcagcacg ttttatatct ttatataact ttttcgaaga
      901 gttaggaaac tgaataatat ctgacatata tgtcacctcg tttgattata aatcttgaag
      961 gattaatgcg ctccacgttt cgaaatcatc atatgactct atgttgcctt tttcaaccca
     1021 ttttattcgt aaagtatctg tttctttagc ttctacatca ttcgtactta cagtgatttt
     1081 aaatacaaca ccaatatgta ccttgcccac ttcattatta tcgtcattaa taaaaccgat
     1141 atattccata ttttgtgaat cttgctcact taaacctact tcttcttcta attctctctg
     1201 tgcattaact ctcaatactt cgttaataga ttctgctcct ggaacatcat tcatatgacc
     1261 gcctacacct attgaagatt gtccatgcaa tcgagcttct ccaccgccag ataatcgttc
     1321 atacactaat atctcgccat gctcattttc aagtaaacaa taagaaatga gttgtttata
     1381 tgatggatcc tcttccatat cgccgcgtcg cttaacttca tattgactta gcgcatcaaa
     1441 aatattttga ccttctggtt tattcttatt taaaaaccca ttgaaagtat tcttttcatt
     1501 attaaaaata atttctctag gtactacaat gatttgttca tcaaatttag acatcgtata
     1561 aaactcctca ctttaatgtt tgttctctta aatgcatttt agcaaatatg tcatatttta
     1621 gcgattaaat gtggcatttt gctttttaaa aaataaaaaa gagatgacaa tgccatctct
     1681 tttttataag tgttattatt ttaaagcatc tttagcttta gttactaatt gagcaaatgc
     1741 tttttcgtca gaaattgcga tttctgataa cattttacgg ttaatgtcga taccagcttt
     1801 tttcaaaccg ttcattaaac gtgagtagct catttcatgt tgacgagctg ctgcgttgat
     1861 acgtgtaatc cataatttac ggaagtcacg tttacgttga cgacggtcac ggaaagcata
     1921 ttgacctgat ttcattactt gttgcttagc tactttgtat aatgtatgtt ttgaaccgaa
     1981 gtaaccttta gctaatttaa tcgttttttt acgacgcgct cttgttactg ttccaccttt
     2041 aactcgtggc ataaaaaatt cctccttagg taattcctac cgatttctgt atttgttctt
     2101 attttttgta tgctaataat tgttttacac gtttcatatc gctcttagac actaatctag
     2161 ctttacgtaa ttgacgtttt tgtttagtgc tcttgtttgc gaataagtga gatgtgaaag
     2221 ctcttgaacg ttttaattga cctgaagcag ttcttttaac acgtttagct gctccgcggt
     2281 gagttttcat ttttggcata atataagtcc tcctgtaata gctttaatta tttttcagct
     2341 gttggcgcta acatgataaa catttgacgc ccgtccattt taggtttttg ttcaactgtt
     2401 gctatatctt tgcattcatc tgcatatttt tctagcacac gttgaccaat ttccttatgc
     2461 gtaatggcac gccctctgaa acgaatagat actttacatt tatcgccttt agttaagaat
     2521 ttacgtccgt ttttcaactt agtttggaaa tcatgttcct caattgttgg acttaaacga
     2581 atttctttaa cattgataat tttttgtttc tttttcattt ctttttcttt tttctgttgt
     2641 tcgaatttga atttaccgta atccataatt cttgcaactg gtggtttcgc attcggtgca
     2701 acgaccacta agtctaaatc tacacgttca gccatttcta aagcttcacg ctttgattta
     2761 acaccaattt gttcaccatc ttgaccgatt aaacgtaatt cttttgcacg aattttgtca
     2821 ttgatttgag tttgatcttt tgctatggtt gacacctcca aaatttttac gaaatttgca
     2881 ccaagcaaaa aggaagagca ggtataaaat acccgctctt ccttatacac agttatgtgt
     2941 aatgtgatta acctgccaac tgctttatgc gtcgctacag gtgagaagcg ggtgcttcta
     3001 cttggttcgt ttcgtattca acgttgttaa tcatatcaac aattcacatt taagtcaaca
     3061 ctataactgt aattattttt attttaacct tttatttcat ccattgacac gtcttgacgt
     3121 aaatctactt gttctaatgg aatttttttc gttttatatc gaagcttatg ataaataaag
     3181 aatgctaaaa atactggaat tcccatatac gtaattaaga agcgactaaa attaaaatct
     3241 cctgttttaa taaagtcaac atcttgacca ataattacta caatacataa aaagccagca
     3301 aataatggtc cgaatgggaa taatttagca gtatatttta atttagattt gtcataattt
     3361 tgtttatcaa atgctcttct aaatcgataa tgacttactg ctatacctac ccaagcaata
     3421 aaaccagtta aaccacttgc tgcaacgata tattcgtatg caccttttgt aaggctttgt
     3481 aatacgaaaa taatcactac aatgattgct gtaacgagta acgacatata tggcacaccg
     3541 tttttatttg ttttaccaaa tgcttcaaac gctaatttat ctttactcat tgaatatagc
     3601 attcgggttg aagcatacat acctgagtta cctgctgata acacagacgt taaaatgact
     3661 gcattcataa atgatgctgc aaacgcaaat ccagcatttt taaacactaa tgtgaatgga
     3721 gacgttgcta cattatcact accacccatt aatgcactac tatcataagg aattaacata
     3781 ccgataacaa aaatggctaa aatgtaaaat aataaaattc tccagaatac ttgtttaatt
     3841 gctttcggca cagcacgttc aggattttct gattcaccag ccgtaatacc aattaactca
     3901 gtaccttgga atgagaaacc agcgattaag aatacaccta gaattgataa caaacttcct
     3961 cctaagttgc caccaagaat aggaccttca cctttattaa atatttcaaa tcctacaaca
     4021 tgaccaccca tgattcctac aatcgttaat aaaccaattg caatgaaaac aataactgta
     4081 atcactttta tcaatgccaa ccagtattca ctttcaccat agacgcgaac tgataacgaa
     4141 ttcagactaa aaattataac taaaaacaac gcactccatg cccaagcggg tatgccttgc
     4201 aatggtgtcc aatattgaat gacttgtgct gcaatcgtaa tatctgctgc tacagttact
     4261 acccagttaa accaatagtt ccaaccaagc gcaaacccta aagatggatc aacaaatctt
     4321 gtagcatatg tactaaatga acctgatact ggtaaatacg tagccatttc accaagtgac
     4381 gtcattaaga aaaataccat tattccgata attgcgtatc ctattaatgc acccaaagca
     4441 cctgcatcat gaattgctcc accagaagtt acaaataaac ctgtaccaat acaaccccca
     4501 atcgcaatca tagaaatatg acgatcttta agtccccttt tgacaacatt gttactttca
     4561 ttttgaactt ttgacatata agctcccttt ccatgtttta gaggccaaga aataaataac
     4621 ctttattcac tttcataccc ttttttacag aacatagtga tattaaaagt aaaaaagtgg
     4681 ctacatacct ttaagatatg caaccacttt ggcaattatc tatataaggt agcacatcac
     4741 attctgtgac agtacagttc ctattcgaaa actgtcccaa tagatataat ttatggttta
     4801 tatctatttc ggcatcttta cctttcactt gttcaactta tgtaccataa atacttctga
     4861 caagttacta attaaacatg caacctctaa ctcaatttaa tattttaact aacttgtaat
     4921 atacaggatt catcacgcat aatcaaccct gtaaaacttg atacgcaata aaagttttaa
     4981 agcattttat tgcgacaact gtctatctat gttttttcaa acgaatttca tcaactagat
     5041 tccagataaa ttcatctttt tcaactgttt cttggtcttg cgatccatat tgacgcacat
     5101 tcacttgatt attttcaact tccttatccc caactacgat ttgataaggt attttttgca
     5161 tttgagcttc tctgatttta taacccattt tttcattacg gtcatcaata cttacacgaa
     5221 caccttgaga tttcaattca tcttgcaatt ggcgcgcata atcataatgt aaatcaacgt
     5281 taactggaat gatttgaact tgttttggcg ctaaccaagt tgggaatgca cctttagttt
     5341 cttcagttaa aaatgctaca aatcgttcca ttgttgatac aacaccacga tgaataacaa
     5401 ctggacgatg atgttcacca tcttgaccaa tataagttaa atcaaaacgt tctggtaata
     5461 agaaatcaag ttgtgctgtt gataatgtct cttctttacc catcgctgtt ttaacttgaa
     5521 catctagttt cggaccatag aatgccgctt caccaatcgc ttcttcgtac gataagccaa
     5581 gctcatccgc tgcctcttta agcatatttt cagctttatt ccacatgtca tcatcatcaa
     5641 agtacttttc tttatcttca gggtctctat aacttaatct aaagctataa tcctcgaaac
     5701 caaagtcttt atacacatca ataatcatgt ttacaacgcg tttgaattct tctttaattt
     5761 gatcaggtcg aacaaagata tgtgaatcat ttaaagtcat accacgaaca cgttgtaatc
     5821 ctgatacagc accacttgct tcatatctat gcatcgttcc tagctcagcg atacggatag
     5881 gtaattcacg atatgaatgt ggtttattcg cataaatcat catatgatgt ggacagttca
     5941 ttggacgtaa taccatagat tcagtttcat ctaactgcat tggtgggaac atatcttctt
     6001 gatagtgatc ccagtgacca gatgttttgt ataaatcaac attagcaagt actggtgtat
     6061 aaacgtggtc ataacccatg ctcacctctt tatcaacaat gtaacgttca atttcacgtc
     6121 taattgttgc accgttaggt aaccataatg gcaaaccagc accaactaat tggctatttg
     6181 tgaataattc taactcttta ccaattttac gatgatcacg ttctttacgc tcttctaaca
     6241 tttgtaaatg tgcttttaat tcttttttat caaagaaagc agtaccgtat atacgttgta
     6301 acattttgtt gttactatct ccacgccagt atgcacctgc tgtagataat agtttaaact
     6361 ctttaatttt agctgttgat ggaacgtgaa ctccacgaca taaatcagta aaatcacctt
     6421 gactatataa tgttacattt tcatcttcag gaatcgcgtc gattaattct aatttgtatt
     6481 catcattgct gaataactct ttcgcttcat ctcgtgaaac cacttttcgt tcgattttca
     6541 tattttcgtt aacgatttgt ttcattgttt tttcaatttg ttcaaagtca tcagatgaga
     6601 tgttttggtc aatgtcgaag tcatagtaga atccaccttc tattacagga cctacaccaa
     6661 atttaacatt accatataac cttttaatcg cgtgtgccat taaatgtgca gtagaatgac
     6721 gtaatacctc taacgcttct tcactacctg gtgtcacaat ttcaattgat ccatcagttt
     6781 caagcggttt agttaaatct acaagttgcc cgttaaattt gccggcaaca gcttttttac
     6841 gtaatccagg actaattgat tgtgctatat cttcagtagt agtaccttta tcaaacgcct
     6901 ttttattacc atctggaaat tgaatattaa tttgttccat gttaaaccct cctatttgct
     6961 ttataaatac ttttatctga cctcgttaaa aaattgaaca acaatcatcg ttttaacatt
     7021 aaacctatta acaataaaca gggaatatat gacaccaaca aacatttatg tctgaaaaaa
     7081 acaaaaaatc ccgcccctat acaagggacg agatcgtcgt ggtaccaccc tagttattta
     7141 atacaattta tgtattaaat atctctgctt aagataacgg tcttgatccg ggtattcatt
     7201 acaaatacca taaatgaagt agtaatcatc taatttatta accatattct catcaaacaa
     7261 tggctttctg tgtaatattg attagatcat cttgtcttca atcatttaaa cgatttagat
     7321 ttgtattcat tatacaccaa tcattttaaa attcaattgt ttctgaaatt ttctcctgat
     7381 aaaaagtatg gtgttgacaa agatttgaca cgttcaataa tacgtgctgc tttagtcttc
     7441 tcttcaccat cacgagtcat cgctaaatga tgttccaatt cactatagtc aaaattagaa
     7501 ctaaagaatg ttggtaattc atgaaccatt cgatagtgta gcaaaggtcc aattacctca
     7561 tctctcaccc atggagtcac ttcttcagcc ccaatatcat caagcattaa aatgtttgct
     7621 tctcttacgc gatgtaattt cttttcaaaa gaaccatctt taaagccacc ttttaatgtt
     7681 ctaataaatt ccggtaaata aataattgtc gaacgtacct tcttagattt gagctgattc
     7741 gcaattgcac ctagaataaa agatttacct gtcccaaatg gaccataaag gtaaaggcct
     7801 ttcacttgtt ccccattagt tattgctgta caaatatcat ctgctgccat agctacatca
     7861 agacggtctc gatgattcat ataaatatct ttcaatttgg catttaaagt atctcgttgc
     7921 atatgatgag atgtaattag ctcagcttca aagcgttctt cgtcgtactt gattttacat
     7981 gggcattgta aatagcgtat tttaattcgg ttattatcaa catataactc aggcacatgc
     8041 ccctttacga aatttggaca atcagcaaat ttatgaccgt cataatgttt ttgttgatct
     8101 ttatactctt gtaacacatt taagtcttca tcaatcatag cattcgttaa ttcagctcga
     8161 tgcgcttcca aaaattgctt aacatctggg tcattgatta cttctttttt tatcttttct
     8221 attctttttt caaagtcctg cgacgtgtta attatacttt taaattgctt cattatcgac
     8281 tgtcctcctc ccatttttta gataatttat ctagaaatgc ttgtcgatct tgctctaatt
     8341 gttgatcatc tacgctatta tctttagccg aatcttcttc actaggttta tctctatttt
     8401 ctaaccattt aggtgttttt tcttttgaaa tacgattacg ttgcccatag tatgaaccac
     8461 gcttttggta atttccgcta gaaccctcat ttttaggttg attaactttt ttagcgtaat
     8521 tatatgcttc tttagctgtc ttaatacctt ttttcttcca atttgatgct atttccaaaa
     8581 tatacgcttt aggaagtttc atatcttctt ttaacatgac aaattgcaac aaaatattaa
     8641 tgacgccaaa agacattttt tcacgttcaa ttaattcttc aaccattgtc ttttgcgata
     8701 tagttggttc tgattcagac caagaagcta acatatcaat tggactcgtt tgttcaagta
     8761 actcaaacca ttcatcactt tgtggctttg gattcacttc tgaagatttg cccgtcgaag
     8821 atgatgtagc aggagatttc acctgtaatt taggcatttg attttcgtgt tccattaaat
     8881 aatacgagcg tgcttgttta cgcatttctt caaaggataa ctgttgtcca cttgtaattg
     8941 aatttaaaat aacatgcttc atgccatctg ctgttaaacc atataaagtc gcgagttgtg
     9001 taattaaacg ctttgcatct ttggtaacaa tgtcttgact aataaaatgt ttacctaaca
     9061 tttgtctcaa catttcaaag tcaaaagatt catttgataa atcgatacct tggtacggtt
     9121 cattaatcgg aatatcactt gtatcgatat ctatttttgt agacggcact ttaaaaacat
     9181 cagtaaattg tcttgttacc tgtttaaatt cactcaaatc aatttgttga tactcaaagt
     9241 atttcttcaa ctcatgaaat cgacgatgct cgacttcact atataaaaag attgacaaca
     9301 ttggatcatt aaaaaataaa tgtgctgaag gcggttgaat taattggtaa acaaattgtg
     9361 tttcttgttc atcatgtttg acaaacgcct ttaacaatcc aatcgcttca agtaagtcca
     9421 tttgttgtct aaactctagt aaattaattt taagttcatt cataaaaata taatgagaaa
     9481 gaatcaatgt ttcattatga ctgtctttaa cgaattgagt cataaaatga tataaaccca
     9541 ctgcttgcgt tccaattagc ggtgtataca gtcgattcaa tacctctaaa tgattcgtat
     9601 ttaaatcaaa gtgttgcata actttgaatt gatcctttgg tcttaagccg aattcgaagg
     9661 cttgtcgtcc catttaagca tcactccgtt tgttttcgct taaaatccct tgcatcgatg
     9721 ctaacaattg atcaacatct ttaaattctt tatagactga tgcaaatcta acatatgaaa
     9781 cttgatcaac atgcattaac aagttcataa cgtgttcacc tatatctcgt gaagacactt
     9841 ccgtatgacc ttcatctcgt aattgccatt caaccttgtt tgttatgtct tcaagttgtt
     9901 gatatctaac tggtcgtttc tcacaagaac gcacaagtcc attaagtatt ttttctcttg
     9961 aaaactgctc tcttgtgcca tcttttttca caactataag ctgactaact tcgatatgtt
    10021 caaatgtagt gaaacgtgtt ccacaatttt cacattctct tcgtcttcga atggcattta
    10081 attcatcggc atgccttgaa tctacaactt tagattgtgt agaattacat ttcgggcatt
    10141 tcattacatc accctcttta ttttgattat gcctaattat actataaatc tagagatgaa
    10201 aaaagaatcc ctcaatttaa ttcatttaac caaataatga aacaataaaa aacattatat
    10261 cgttacttat taagtaattt gcatgacaat attattgtat taaaaaataa aaaacctaac
    10321 tccgaagtca gagttaggct ataaattaat tgtattaact tgcacttaca gtttcttttg
    10381 atgtcaaaag tgctccaatt tgctcagcaa catctacaac tctatttgaa taaccccatt
    10441 cattatcata ccaagcaata acttttactt tattccctga catgaccatt gttgattttg
    10501 catcaataat agctgaattt ggattagtat taaaatcaac agacactagt ggttgatgtt
    10561 cgacttctat gataccttct aaacctgcat tttcaaaagc ttggtttact tcttctgcag
    10621 ttacttcttt ttctaaatca acaactaaat caacgagcga tacattcttt gttggtacac
    10681 gtaatgccat gccgtgtaat ttaccttcta attctggtaa tacttctttt aaagctttcg
    10741 ccgcaccagt agaagtagga ataatgcttt cattacatga acgtgcacgt cttaaatctt
    10801 tatgtggatt atcaatattt ttttggtcat ttgtaatagc gtgaacagta gtcattaaac
    10861 cattaactat tccaaactga ttatttaaaa cttttgcaac tggaccaatg caattagtag
    10921 tacatgaagc attactaaaa atgtcaaatg cttctatatc taattggtta tcatttacgc
    10981 ctttaactac catttgaaca tgtccacctt ttgaaggacc agttaacaat acttttttgg
    11041 cacctgcttt aatatgtgcg atggctttat caccatgatt aaatttacca gttgcatcta
    11101 tagcaatatc gatatctaat tctttccatg gcaagttttc aggattgcga tcagcaacca
    11161 atttaatttt atgatcacca acttgcaatc cattttcaat cggttcaact tttagattat
    11221 attttccatg tgttgtatcg taattgatta aatgtgcaat tgtttcgggt ggataactag
    11281 catttatcgc tactacattt aaatttttat tttgtaatgc aatacgtaat accattcttc
    11341 caattctacc cataccatta attgcaatat tcgttgacat taaaagcacc ccttgatttt
    11401 tatgtgttat actttatgga attagtatag catattatat gaattttgaa agcgttttcc
    11461 gttgatttat ttatcttttt tataaaaaat agtcattact tacatacatc aacgcgtacg
    11521 taaagtaatg acttatttat agtgtaatat taatcttctt ctccgtaatt cggcttttca
    11581 atataacctt cttcttctaa caatctctca aggttttgtt ttaattcaag tttatcccct
    11641 aaattatcga taacatgatc ggccattcgg ctttttttat caatagaaat ttggctatag
    11701 acacgtgctt tcgcatcttc taatgacaga ttattacgtt gcattaaacg atccatttgt
    11761 atactttcag aagtgtatac aacccacact tcgtctactg tattttccaa ttcattttca
    11821 aataataatg gaatatccat gattacatta tatccttgtt ttaaatattc ttgcttttct
    11881 tcttccataa tatctcgcac gataggatgt atgatagcat ttaattctaa gcgtttttct
    11941 ggatggttaa acactaaatc acccatataa cgacgattca tctcaccatt ttcatcaatt
    12001 gcttcatcac caaagacttc tcgtacttga gctaaacctt tactcccctt tttaacagct
    12061 tccctggctg ctttatcagc atctactact ttaaaaccga atacggataa gagttctgat
    12121 actgttgatt ttcctgaggc gattccacct gttagaccaa taactttcgg cataatttca
    12181 ctctttcttt atttttgaca tactggacaa taatgactat ttcttgtcgc gatgattttt
    12241 gtttcaattt gacttccaca cactttgcat accggctgct tatatacatt aagatgcaat
    12301 tgcatctcac cagtttttcc atcagcatga cgataatctg aaatacttgt accgccatgc
    12361 ttaatacctt cttctagtac ttctctaaca taataaaaaa ccatttcttg ttgttggtgt
    12421 gttaagtctt ttactttttt atctggtaaa acacctgcac gaaacaacgc ttcacatgcg
    12481 taaatatttc cgcaacctgc gattacttta tgatccaaaa tcacttgttt gattggttta
    12541 ttcttattag actgttgatg aattcgattt aaataatacg tcaatgcttc atttgtaaaa
    12601 ggttcaggcg ctatttctaa aaatgaagga taagatgcta cagacgcaac atttctaatt
    12661 tctccaaaac gacgtatatc tgaataaatt aactttttgt catttgacaa ctcaaaaata
    12721 acatgccaat gcttacgata attaggtatc ataatatctt caagttcatc tacaatgaaa
    12781 aaaccacccg ccatacctaa atgactaatt aatgtacgtt gttctcgttt attatctagc
    12841 tgaaaaacga tatatttact tcttcgttct acatttgtaa tggtatagcc ttccgataaa
    12901 gttttaaaag tatctaattc aattcctttt ataattgttt ccttgccttg agctttacct
    12961 tcgattactt tatccgaaaa tataacgtgt tcaatttttt gatttataac gtagggttca
    13021 attcctcttt ttacatgttc tacttctggt aattcgggca taccattaac ctcactttat
    13081 tttgcatcat accaagttgc accataactt gagtctactt ttaatggaac atctaattgc
    13141 aatgcatttt ccattatctc ttctacaaat tcactaaatg aatctacttc tgacttaggt
    13201 acttcaaaaa ttaattcatc gtgtacttgt aataatagtt tagcttgata tgttgtctct
    13261 ttcatttttt gtgcaaattt aaccattgcc agtttaatga tatctgcagc actgccttgt
    13321 attggcgtat tcatagcagt acgttcagca aagccgcgta aattaaagtt acgactcgta
    13381 atatcaggaa tatagcgtcg acgatgtagc aatgtttcca cgtaacctaa agctttggca
    13441 tcttttacaa tatcagacat atattgtttc acacctggga aactagctaa ataatcatca
    13501 atgaatgctt ttgctttttt acgagtaata cctaaacttt gacttaaacc ataatcactt
    13561 atcccataaa caattccaaa gttaaccgct tttgcttgac gacgcattaa actatcgact
    13621 tgatcagcct ctacaccaaa tactttcata gcagttgctg tatgaatatc atcgccgttg
    13681 ataaatgctt ctttcatact ctcatcttgt gtaatgtgtg ctaatacacg caattcaatt
    13741 tgagaataat ctgctgataa tataacgcta tcttttgaag ttggtttaaa ggcttttctt
    13801 attttacgcc cttcttcaag tctaaccgga atattttgta aattaggatc tacacttgat
    13861 aaacgtccag tttgcgctaa agtttggtta aaacgtgtat ggatacgttg atcatcacta
    13921 attacctttt gcaatccttc aacataagta gactgtaact tagataattg acgatattct
    13981 aaaatataat caatgatagg atgttcacct tgcaattgct ctaagacatc tacagcagta
    14041 gaatatcctg ttttcgtctt tttaataaca ggtaattgta atgtctcaaa caatacaaca
    14101 cctaattgtt taggagaatt tatattaaaa tcttcaccag ctgcatcatg gatatttcga
    14161 atcaagacgt ctaatttttc ttgaatttct ttttccattt cttctaaatc atgaacatct
    14221 gtaaatatac caatttcttc catttcactt aaaatcttag ctagcggtag ctctaaatca
    14281 gctaagagtt ctacctgatt gtattcttct aattgtttat ccatatttgg tttcgcaaag
    14341 taaattgcat cagtaataga agcaacatat ggatttaaaa catcatcttc aggtacctta
    14401 aatttcttac cttttccata tatacttaca tcgtctttca caaaactttg accgtacaat
    14461 gaaacaactg attgaacatc actaatcgta cgagatggat caattatata actggccaac
    14521 ataatatcga aagaaatatt ttgaatatca attcccaatc tatgtgatgc tacatatgtt
    14581 tttttagcat catatacgac ttttttcgta ttcggatttt ctaaccatga aactagttcg
    14641 gcataattat ttatgtcatc cgcattaatt acaatatgtt tctcacctgt aaataaagag
    14701 aattttaaaa tattatttcg caaatagtta ccaccgtcta attcgaaatg gatggccgct
    14761 tctttcaatg aagtaaaatc aatattatca aaagacgttt caatttcaaa tgtcttttct
    14821 attgcatctt caacgcttgc tgattgatca atgtcagaca acaattgttt gaattctaac
    14881 ttcttaaaca attcgatttt ttcttgttgt tcatcttgat gagtcattaa cgtatcttca
    14941 agttttactt caatcggact atctacatta atcgttgcta attctttact cattaatgca
    15001 tcttctttgc tattttgaag tttttctttt aactttttac ctgaaatttc atctaaatgt
    15061 tcatagacac cttctactgt gtcaaattgg tttagcaatt ttattgctgt tttctctcca
    15121 acacctgcaa cacctggtat attatcagaa gtatctccca ttaatccttt catatcaata
    15181 atttgattag gtgttaaccc gttgtatttt tccgcaataa agtcaggtgt ataatgatca
    15241 acatcagtaa cacctttttt agtgtaataa atggttacat tatccgttgc aagttgtgtt
    15301 aaatctcggt ctcccgttat aataattgtc tgaaagcccg ctttatctgc ttctttactt
    15361 aaagttccga taatatcatc tgcctcatag ttatctaatt cataacgttt aatatgataa
    15421 gcatctaata attggcgtat ataaggaaat tgctcactta gttcaggcgg cgttttctgg
    15481 cgtccacctt tatattcact atatttttca tgtctgaaag ttgttttacc tgcatcaaac
    15541 gctactaaaa aatgatttgg cttttcttct tttaaaatct tctctagtaa cattgcaaaa
    15601 ccatatactg cattggtatg aatgcctgct ttgtttgata acaaaggtaa tgcataaaaa
    15661 gctctaaaac ttaagctatt accatcgatt aatactaatt tattcacaat tttaacctcc
    15721 agaactaatt tatatatatt ttagcatatt cgactgtgat gtcgtaatta aagaaactat
    15781 ctcatagagt atttatacac gacctaattt caaataaaac aagtgcctat atagacttag
    15841 ctaaaaaata aaaaagacat aatcatgatt gttcttgatg agcactccat aaagtcaggt
    15901 taaaagtcca acttttgaga tgcacattat catttcatga ttatgtctac tttttaatat
    15961 ttttatgctg ctattttaaa tttttatagg taagaataag ttatgaatcg tctgaaccaa
    16021 atggaatata tttaactttg gtcattttcg gtaattcttc ataattatta aaccactctt
    16081 ggtaattttt attgattggt gacggatgat gcatataatg ttcaaacggc aatgattcca
    16141 ctgtataaac ctcttgtcgt ggatgatctt taatttgttg ttttaataac tgaactcttt
    16201 tttcatgttc ataatgaaca taaataaatg cactaagata tatcacagct aaaactagtg
    16261 atgcaccttt aataaaatta acattgattg acttatattt ccgaaattct tttaataaaa
    16321 taattaaaat gattacatgt atcgtataaa caatcaaaaa attacctggt tctattggag
    16381 taacaatgac tagtgtcgac gccgaaacac atattgcaat aagtaaggaa tataaagtga
    16441 tttgtgtttt acgatcatta atagacaaat aaatacctac aaatatcgaa aacgcaaagt
    16501 aaccacatac aattacgttc acaaaaccaa ccaatccaat atctgtattt ttatttaata
    16561 aaaactgatt gtaaaatagt aaataataaa gcggtaaagt gataaatcct atcataatga
    16621 tacgacgctt taatattgtt aaatgtacat accgatcact tctaagcagt agataaataa
    16681 taagcgctgc aatcatactt aaaattataa tctgactgaa aatgacacca tatggaagtg
    16741 acgttgatat catttctgca aattttgaaa agataccttg attatttgaa acttgttgat
    16801 attctgatcc ttcaaataaa attttgcgat aatttggatt ggaaaacata atgattgtac
    16861 ctatagtagc aatcataaat gaaaacaata atttataatt aagtgttcga tttgcagtaa
    16921 attcatacag aaaagctaaa attaaaatga tacagtggaa taatgtcaca ttctccataa
    16981 ataattgacc gaagaagcat aaaacataga atagtacagt aacacttacc ggctgctttt
    17041 ctttatatat aattgcatta atacaataat aaataataaa gagtgaaatt agtgttgatg
    17101 tcgcataatt ataaaatcct gcaaaccagc catatgtatc tgcataaata gcacttggta
    17161 aaattaacat taaagaaaat gctaacaaat aatagcttgt ccacgcttta caacgtgtaa
    17221 tgtgcataat catccaaatg atgcccatac taatgagacc atatgaaagc caacgtaacc
    17281 agcttacatg tacagctata atttcaaaga tatttccgat atagcgacca ttaagtgatg
    17341 caaatcctac ttttaaaata tcagtattat aattgctaaa ccattgtaaa tcatcgtgca
    17401 tgattggtag taagataccc ataaaagtat aaaacaataa tatcgcaatt aatatcaaag
    17461 ttgtcttgtg taattgaatt gttttcactt tgctaatcct caaatctagt taaattttcc
    17521 tcaacttgta ggtcgaaaaa ttaattcaat atttttaatg tatttctaat tttcacctat
    17581 gcatgtttcc tcaatcaaat tagataaaca aggtatttaa tattactttc aacaatttat
    17641 ctaaatcgcc cctcgtcttt ttctatgacg aatgattaca cttgtgtatt caagtgttca
    17701 aattaagatt tatttccgtt caaataaaac ttattacttt ttaattaagc atctatttta
    17761 agcgcattac taaaatttct ttattataat aaatatatta
//
LOCUS       DAHHSC010000036        17626 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.036, whole genome shotgun sequence.
ACCESSION   DAHHSC010000036 DAHHSC010000000
VERSION     DAHHSC010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 17626)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17626)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17626
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.036"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            complement(<1..229)
                     /locus_tag="NBI57_000936"
     CDS             complement(<1..229)
                     /locus_tag="NBI57_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulokinase"
                     /protein_id="HCD1821900.1"
                     /translation="MSYSIGIDYGTASGRVFLINTTNGQVVSKFVKPYTHGVIESELN
                     GLKIPHTYALQNSNDYLEIMEEGISYIVRESK"
     gene            complement(388..1266)
                     /gene="hchA"
                     /locus_tag="NBI57_000937"
     CDS             complement(388..1266)
                     /gene="hchA"
                     /locus_tag="NBI57_000937"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein deglycase HchA"
                     /protein_id="HCD1821901.1"
                     /translation="MSQDVNELSKQPTPDKAEDNAFFPSPYSLSQYTAPKTDFDGVEH
                     KGAYKDGKWKVLMIAAEERYVLLENGKMFSTGNHPVEMLLPLHHLMEAGFDVDVATLS
                     GYPVKLELWAMPTEDEAVISTYNKLKEKLKQPKKLADVIKNELGPDSDYLSVFIPGGH
                     AAVVGISESEDVQQTLDWALDNDRFIVTLCHGPAALLSAGLNREKSPLEGYSVCVFPD
                     SLDEGANIEIGYLPGRLKWLVADLLTKQGLKVVNDDMTGRTLKDRKLLTGDSPLASNE
                     LGKLAVNEMLNAIQNK"
     gene            complement(1536..2723)
                     /locus_tag="NBI57_000938"
     CDS             complement(1536..2723)
                     /locus_tag="NBI57_000938"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HCD1821902.1"
                     /translation="MVQSLHEFLEENINYLKENGLYNEIDTIEGANGPEIKINGKSYI
                     NLSSNNYLGLATNEDLKSAAKAAIDTHGVGAGAVRTINGTLDLHDELEETLAKFKGTE
                     AAIAYQSGFNCNMAAISAVMNKNDAILSDELNHASIIDGCRLSKAKIIRVNHSDMDDL
                     RAKAKEAVESGQYNKVMYITDGVFSMDGDVAKLPEIVEIAEEFGLLTYVDDAHGSGVM
                     GKGAGTVKHFGLQDKIDFQIGTLSKAIGVVGGYVAGTKELIDWLKAQSRPFLFSTSLA
                     PGDTKAITEAVKKLMDSTELHDKLWDNAQYLKNGLSKLGYDTGESETPITPVIIGDEK
                     TTQEFSKRLKDEGVYVKSIVFPTVPRGTGRVRNMPTAAHTKDMLDEAIAAYEKVGKEM
                     KLI"
     gene            2894..4069
                     /locus_tag="NBI57_000939"
     CDS             2894..4069
                     /locus_tag="NBI57_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HCD1821903.1"
                     /translation="MLDWFQLANNKENKTIQLRRYLHQYPELSFEEFQTHDYIVNQLS
                     QLSCDIETPIGRNGIKATFKGLGTGPTIALRADFDALPVEELNDVPYKSKNPGCMHAC
                     GHDGHTAILLTVAEILDEHKHLLEGNVVLIFQYGEEIMPGGSQEMIDAGCLENVDRIY
                     GTHLWSGYPTGTIHSRAGAIMASPDEFSVTIKGRGGHGAKPHETIDPIVIMAEFILSA
                     QKIISRTIDPVKQAVLSFGMIQAGTTDSVIPDQAFCKGTVRTFDSDIQNHVMDKMDKL
                     LQGLAIANDINYDLNYIKGYLPVHNNEKAYQVIKEATNDLHVRFNESDLMMIGEDFSH
                     YLKVRPGAFFLTGCGNESKGITAPHHNPKFDIDEKSLKYAVAVFLKIIELEQVFKTN"
     gene            complement(4351..5535)
                     /gene="tuf"
                     /locus_tag="NBI57_000940"
     CDS             complement(4351..5535)
                     /gene="tuf"
                     /locus_tag="NBI57_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HCD1821904.1"
                     /translation="MAKEKFDRSKEHANIGTIGHVDHGKTTLTAAIATVLAKNGDSVA
                     QSYDMIDNAPEEKERGITINTSHIEYQTDKRHYAHVDCPGHADYVKNMITGAAQMDGG
                     ILVVSAADGPMPQTREHILLSRNVGVPALVVFLNKVDMVDDEELLELVEMEVRDLLSE
                     YDFPGDDVPVIAGSALKALEGDAQYEEKILELMEAVDTYIPTPERDSDKPFMMPVEDV
                     FSITGRGTVATGRVERGQIKVGEEVEIIGLHDTSKTTVTGVEMFRKLLDYAEAGDNIG
                     ALLRGVAREDVQRGQVLAAPGSITPHTEFKAEVYVLSKDEGGRHTPFFSNYRPQFYFR
                     TTDVTGVVHLPEGTEMVMPGDNVEMTVELIAPIAIEDGTRFSIREGGRTVGSGVVTEI
                     IK"
     gene            complement(5752..7833)
                     /gene="fusA"
                     /locus_tag="NBI57_000941"
     CDS             complement(5752..7833)
                     /gene="fusA"
                     /locus_tag="NBI57_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017804675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HCD1821905.1"
                     /translation="MAREFSLEKTRNIGIMAHIDAGKTTTTERILYYTGRIHKIGETH
                     EGASQMDWMEQEQDRGITITSAATTAAWEGHRVNIIDTPGHVDFTVEVERSLRVLDGA
                     VTVLDAQSGVEPQTETVWRQATTYGVPRIVFVNKMDKLGANFEYSVSTLHDRLQANAA
                     PIQLPIGAEDEFEAIIDLVEMKCFKYTNDLGTEIEEIEIPEDHLDRAEEARASLIEAV
                     AETSDELMEKYLGDEEISVSELKEAIRQATTNVEFYPVLCGTAFKNKGVQLMLDAVID
                     YLPSPLDVKPIIGHRASNPEEEVIAKADDSAEFAALAFKVMTDPYVGKLTFFRVYSGT
                     MTSGSYVKNSTKGKRERVGRLLQMHANSRQEIDTVYSGDIAAAVGLKDTGTGDTLCGE
                     KNDIILESMEFPEPVIHLSVEPKSKADQDKMTQALVKLQEEDPTFHAHTDEETGQVII
                     GGMGELHLDILVDRMKKEFNVECNVGAPMVSYRETFKSSAQVQGKFSRQSGGRGQYGD
                     VHIEFTPNETGAGFEFENAIVGGVVPREYIPSVEAGLKDAMENGVLAGYPLIDVKAKL
                     YDGSYHDVDSSEMAFKIAASLALKEAAKKCDPVILEPMMKVTIEMPEEYMGDIMGDVT
                     SRRGRVDGMEPRGNAQVVNAYVPLSEMFGYATSLRSNTQGRGTYTMYFDHYAEVPKSI
                     AEDIIKKNKGE"
     gene            complement(7956..8426)
                     /gene="rpsG"
                     /locus_tag="NBI57_000942"
     CDS             complement(7956..8426)
                     /gene="rpsG"
                     /locus_tag="NBI57_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013054923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HCD1821906.1"
                     /translation="MPRKGSVPKRDVLPDPIHNSKLVTKLINKIMLDGKRGTAQRILY
                     SAFDLVEQRSGRDALEVFEEAINNIMPVLEVKARRVGGSNYQVPVEVRPERRTTLGLR
                     WLVNYARLRGEKTMEDRLANEILDAANNTGGAVKKREDTHKMAEANKAFAHYRW"
     gene            complement(8492..8905)
                     /gene="rpsL"
                     /locus_tag="NBI57_000943"
     CDS             complement(8492..8905)
                     /gene="rpsL"
                     /locus_tag="NBI57_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001142337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HCD1821907.1"
                     /translation="MPTINQLVRKPRQSKIKKSDSPALNKGFNSKKKKFTDLNSPQKR
                     GVCTRVGTMTPKKPNSALRKYARVRLSNNIEINAYIPGIGHNLQEHSVVLVRGGRVKD
                     LPGVRYHIVRGALDTSGVDGRRQGRSLYGTKKPKN"
     gene            complement(9003..9257)
                     /locus_tag="NBI57_000944"
     CDS             complement(9003..9257)
                     /locus_tag="NBI57_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal L7Ae/L30e/S12e/Gadd45 family protein"
                     /protein_id="HCD1821908.1"
                     /translation="MSKEKVARFNKQHFVVGLKETLKALKKDQVTSLIIAEDVEVYLM
                     TRVLSQINQKNIPVSFFKSKHALGKHVGINVNATIVALIK"
     gene            complement(9394..12990)
                     /gene="rpoC"
                     /locus_tag="NBI57_000945"
     CDS             complement(9394..12990)
                     /gene="rpoC"
                     /locus_tag="NBI57_000945"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HCD1821909.1"
                     /translation="MKIGLASPEKIRSWSFGEVKKPETINYRTLKPEKDGLFCERIFG
                     PTKDWECSCGKYKRVRYKGMVCDRCGVEVTKSKVRRERMGHIELAAPVSHIWYFKGIP
                     SRMGLLLDMSPRALEEVIYFASYVVVDPGPTGLEKKTLLSEAEFRDYYDKYPGQFVAK
                     MGAEGIKDLLEEIDLDEELKLLRDELESATGQRLTRAIKRLEVVESFRNSGNKPSWMI
                     LDVLPIIPPEIRPMVQLDGGRFATSDLNDLYRRVINRNNRLKRLLDLGAPGIIVQNEK
                     RMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSHMLKGKQGRFRQNLLGKRVDYSGRSV
                     IAVGPSLKMYQCGLPKEMALELFKPFVMKELVQREIATNIKNAKSKIERMDDEVWDVL
                     EEVIREHPVLLNRAPTLHRLGIQAFEPTLVEGRAIRLHPLVTTAYNADFDGDQMAVHV
                     PLSKEAQAEARMLMLAAQNILNPKDGKPVVTPSQDMVLGNYYLTLERKDAVNTGAIFN
                     NTNEVLKAYANGFVHLHTRIGVHASSFNNPTFTEEQNKKILATSVGKIIFNEIIPDSF
                     AYINEPTQENLERKTPNRYFIDPTTLGEGGLKEYFENEELIEPFNKKFLGNIIAEVFN
                     RFSITDTSMMLDRMKDLGFKFSSKAGITVGVADIVVLPDKQQILDEHEKLVDRITKQF
                     NRGLITEEERYNAVVEIWTDAKDQIQGELMQSLDKTNPIFMMSDSGARGNASNFTQLA
                     GMRGLMAAPSGKIIELPITSSFREGLTVLEYFISTHGARKGLADTALKTADSGYLTRR
                     LVDVAQDVIVREEDCGTDRGLLVSDIKEGTEMIEPFIERIEGRYSKETIRHPETDEII
                     IRPDELITPEIAKKITDAGIEQMYIRSAFTCNARHGVCEKCYGKNLATGEKVEVGEAV
                     GTIAAQSIGEPGTQLTMRTFHTGGVAGSDITQGLPRIQEIFEARNPKGQAVITEIEGV
                     VEDIKLAKDRQQEIVVKGANETRSYLASGTSRIIVEIGQPVQRGEVLTEGSIEPKNYL
                     SVAGLNATESYLLKEVQKVYRMQGVEIDDKHVEVMVRQMLRKVRIIEAGDTKLLPGSL
                     VDIHNFTDANREAFKHRKRPATAKPVLLGITKASLETESFLSAASFQETTRVLTDAAI
                     KGKRDDLLGLKENVIIGKLIPAGTGMRRYSDVKYEKTAKPVAEVESQTEVTE"
     gene            complement(13154..16705)
                     /gene="rpoB"
                     /locus_tag="NBI57_000946"
     CDS             complement(13154..16705)
                     /gene="rpoB"
                     /locus_tag="NBI57_000946"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499096.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HCD1821910.1"
                     /translation="MAGQVVQYGRHRKRRNYARISEVLELPNLIEIQTKSYEWFLREG
                     LIEMFRDISPIEDFTGNLSLEFVDYRLGEPKYDLEESKNRDATYAAPLRVKVRLIIKE
                     TGEVKEQEVFMGDFPLMTDTGTFVINGAERVIVSQLVRSPSVYFNEKIDKNGRENYDA
                     TIIPNRGAWLEYETDAKDVVYVRIDRTRKLPLTVLLRALGFSSDQEIVDLLGDNEYLR
                     NTLEKDGTENTEQALLEIYERLRPGEPPTVENAKSLLYSRFFDPKRYDLASVGRYKTN
                     KKLHLKHRLFNQKLAEPIVNTETGEIVVEEGTVLDRRKIDEIMDVLESNANSEVFELH
                     GSVIDEPVEIQSIKVYVPNDDEGRTTTVIGNAFPDSEVKCITPADIIASMSYFFNLLS
                     GIGYTDDIDHLGNRRLRSVGELLQNQFRIGLSRMERVVRERMSIQDTESITPQQLINI
                     RPVIASIKEFFGSSQLSQFMDQANPLAELTHKRRLSALGPGGLTRERAQMEVRDVHYS
                     HYGRMCPIETPEGPNIGLINSLSSYARVNEFGFIETPYRKVDLDTHAITDQIDYLTAD
                     EEDSYVVAQANSKLDENGRFMDDEVVCRFRGNNTVMAKEKMDYMDVSPKQVVSAATAC
                     IPFLENDDSNRALMGANMQRQAVPLMNPEAPFVGTGMEHVAARDSGAAITAKHRGRVE
                     HVESNEILVRRLVEENGVEHEGELDRYPLAKFKRSNSGTCYNQRPIVAVGDVVEYNEI
                     LADGPSMELGEMALGRNVVVGFMTWDGYNYEDAVIMSERLVKDDVYTSIHIEEYESEA
                     RDTKLGPEEITRDIPNVSESALKNLDDRGIVYIGAEVKDGDILVGKVTPKGVTELTAE
                     ERLLHAIFGEKAREVRDTSLRVPHGAGGIVLDVKVFNREEGDDTLSPGVNQLVRVYIV
                     QKRKIHVGDKMCGRHGNKGVISKIVPEEDMPYLPDGRPIDIMLNPLGVPSRMNIGQVL
                     ELHLGMAAKNLGIHVASPVFDGANDDDVWSTIEEAGMARDGKTVLYDGRTGEPFDNRI
                     SVGVMYMLKLAHMVDDKLHARSTGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGA
                     AYTLQEILTYKSDDTVGRVKTYEAIVKGENISRPSVPESFRVLMKELQSLGLDVKVMD
                     EQDNEIEMTDVDDDDVVERKVDLQQNDAPETQKEVTD"
     gene            complement(16920..17528)
                     /locus_tag="NBI57_000947"
     CDS             complement(16920..17528)
                     /locus_tag="NBI57_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCD1821911.1"
                     /translation="MSHYYDEDPSVISNEQRIQYQLNHHKIDLITDNGVFSKDKVDYG
                     SDVLVQTFLKAHPPGPSKRIADVGCGYGPIGLMIAKVSPHHSITMLDVNHRALALVEK
                     NKKLNGIDNVIVKESDALSAVEDKSFDFILTNPPIRAGKETVHRIFEQALHRLDSNGE
                     LFVVIQKKQGMPSAKKRMNELFGNVEVVNKDKGYYILRSIKA"
BASE COUNT         5283 a         3457 c         2831 g         6055 t
ORIGIN      
        1 tttttgattc acgtactata tatgatattc cttcttccat aatttctaaa taatcattac
       61 tattttgaag tgcatatgta tgtggtattt tcaaaccatt taattcactt tcaatgacac
      121 catgtgtata tggtttcaca aattttgata ctacttgacc gttagttgta ttaattaaaa
      181 acacacggcc tgaagcagtt ccataatcaa ttccaatgct atacgacatc atgtttcctc
      241 catatcgcta gttgcggtta ttagttttgc aagcgttttc ttaaatcata ctcataatag
      301 gttctatttt acaagtatca cttaaatttt gccaaaaaaa agagtcctca tacatttaca
      361 tatgaggctc ctctaattaa tatttaatta tttattttgt attgcattta acatttcatt
      421 aactgctaat tttcctaact catttgaagc taaaggactg tcacctgtta ataatttacg
      481 atcttttaac gttcttcctg tcatatcgtc gttaactact tttaatcctt gtttagttaa
      541 taaatcagca actaaccatt tcaagcgtcc aggtaaataa cctatttcaa tatttgcacc
      601 ttcatctaat gagtcaggga agacacaaac agagtatcct tctaatggag atttttctct
      661 gttaagccct gctgaaagta gtgctgctgg tccatgacat aatgtaacta taaagcggtc
      721 attgtctaat gcccaatcta atgtttgttg aacgtcctca ctttcagaaa taccaacaac
      781 tgcagcatgt ccgcctggga taaagacaga taaatagtct gaatcaggtc ctaattcatt
      841 tttaatcaca tctgctaatt tttttggctg ttttaatttt tctttcaatt tattataagt
      901 acttataact gcctcgtctt cagttggcat agcccataat tctaatttaa ctggataacc
      961 agataatgtc gcaacatcaa cgtcaaaacc tgcttccatt aaatgatgta aaggtaataa
     1021 catttcaaca ggatgattac ccgtagagaa catttttcca ttttccaata atacatatct
     1081 ctcttcagca gcaatcatca atactttcca tttaccatct ttataggcac ctttgtgttc
     1141 aacaccatca aaatctgttt taggtgctgt atattgacta agggaatatg gtgatgggaa
     1201 aaatgcgtta tcttctgctt tatctggcgt tggttgctta cttaattcat ttacatcttg
     1261 tgacatagtt tattcctact ttctctatat agttactact agtatagaca tatactacac
     1321 actataaaca tgtattacta ttaaaatact cattatcaaa aataatcaaa atgaatttct
     1381 ttgtaaaaaa tacaactaaa ttctacaaga tgcttttaaa attcaaactt caattttggg
     1441 gagcactaaa cattgttata agaacttact gttaataaac taaacattaa aaaaagccca
     1501 cgacaatatt tgccgtggga ataaataaat aaatattaaa tcaacttcat ttcttttcct
     1561 actttttcat aagccgcaat tgcttcatct aacatgtctt ttgtatgtgc agctgtaggc
     1621 atatttctta cacgtcctgt acctcttggt actgttggga aaacgataga tttcacatag
     1681 acaccttcgt cttttaaacg cttactaaat tcttgagttg ttttttcatc accaataatt
     1741 actggtgtaa ttggagtttc tgactcacct gtatcatatc ctaattttga caatccattt
     1801 tttaaatatt gtgcattgtc ccataattta tcatgtaatt cagttgaatc cattaacttt
     1861 ttaactgctt cagttattgc tttggtatcc ccaggtgcta atgatgtaga gaataagaat
     1921 ggtcgtgatt gtgcttttaa ccaatctatt aactcttttg tacctgctac atagccgcca
     1981 acgacaccaa ttgctttaga aagcgtacct atttggaaat cgattttatc ttgtaaacca
     2041 aaatgtttaa ccgtaccagc gcctttaccc ataacacctg aaccatgagc gtcgtcaaca
     2101 taagttaata aaccaaattc ttctgcaatt tctacaattt caggtaattt agccacatca
     2161 ccatccatac taaaaacgcc atcagtgata tacatcactt tattgtattg acctgattca
     2221 actgcttctt ttgctttcgc acgtaaatca tccatgtctg aatggttaac tcgaataatt
     2281 ttagctttag ataagcgaca tccatcaata attgatgcat gattaagctc atctgataaa
     2341 atagcatcat ttttattcat gacagctgaa atagcagcca tattacaatt aaatcctgac
     2401 tgataagcta ttgcagcttc tgttccttta aattttgcta gtgtttcttc taattcgtcg
     2461 tgtaaatcta atgtaccatt gattgtacga acagcgcctg cacctacacc atgtgtatca
     2521 atagctgctt ttgcagctga tttcaaatct tcatttgttg ctagtcctaa ataattattt
     2581 gaagataagt taatgtatga tttcccattg attttgattt ctggtccgtt tgcaccttca
     2641 attgtatcta tttcattata caaaccattt tcttttagat aatttatatt ttcctctaaa
     2701 aactcatgta atgattgaac cacagtatat aatccccctt aagtattgtt tggtaatacc
     2761 tttatcatac agtaattcta cataacttga aagccttatc attcatagat aaaatatctc
     2821 aaagcaatca tttagtacga taatttcata aaatgttata ctataaacat tattttagaa
     2881 agtagtgata agggtgttag attggttcca actagcaaat aataaagaaa ataaaacaat
     2941 tcaactcaga cgttatttac accaatatcc tgagttatct tttgaagaat ttcaaacaca
     3001 tgattatatt gttaaccaat taagccagtt atcttgtgat attgaaactc ctatcggccg
     3061 caatggtatc aaagccacat ttaaaggctt gggcacagga cctaccattg cattaagagc
     3121 tgactttgat gcgttacctg tcgaggaatt aaacgatgtg ccttataagt ctaaaaatcc
     3181 aggatgtatg catgcgtgtg ggcatgatgg tcatacagca attttactta ctgtagcaga
     3241 gattttagat gaacataaac atttattaga aggtaatgtt gtattaatat tccagtacgg
     3301 tgaagaaatc atgccaggtg gttctcaaga aatgatagat gctggatgtc tggaaaatgt
     3361 agatagaatt tacggtacac atttatggag tggttatcct actggaacta ttcattcacg
     3421 tgcgggggca atcatggcct ctccagatga atttagcgtc acaattaaag gtcgtggtgg
     3481 tcatggtgcc aaacctcatg aaaccattga cccaatagta atcatggcag agttcatttt
     3541 aagtgcacaa aaaataattt cacgtacgat cgatccagtt aaacaagctg tactttcatt
     3601 tggaatgatt caagcaggta ccacagatag cgttattcct gatcaagcat tttgtaaagg
     3661 tacagttaga actttcgatt cagatataca gaatcatgta atggacaaaa tggataagct
     3721 tctacaaggt ttagcaatag caaatgatat taactatgat ttgaattata ttaaagggta
     3781 tttgcctgtc cataataacg agaaagcata tcaagttatt aaagaagcaa caaatgactt
     3841 acatgtacgc tttaatgaat cagatttaat gatgattgga gaagattttt cacattattt
     3901 gaaagtacga ccaggtgcat tctttctgac tggttgtggt aatgaaagta aaggtattac
     3961 agcgccacac cataatccca aatttgatat tgatgaaaaa tcattaaaat acgctgtagc
     4021 tgtattttta aaaatcattg aattagagca agtatttaaa acaaattaag catttaaatt
     4081 aattgaaaga tatgaatctg ggacataaac taatgaaatg ctcccttcaa agtagacatt
     4141 gaaaaaatga aaactttgaa gggagcattt tacaatgtcc aagactcaac aattttattt
     4201 aaacagtatt atcagttata agttttatta gtcagtcttt catcaattat tgttcttcta
     4261 aatcgaatag ccattacaaa atttacacat taaaaaaaga cccctcgatt gagggatctt
     4321 ctttttatat aaaatctaag aaattagaaa ttatttaatg atttcagtaa caacgcctga
     4381 tcctacagta cgtccacctt cgcggattga gaaacgagta ccgtcttcaa tcgcgattgg
     4441 agcgattaat tctactgtca tttcaacgtt atcaccaggc attaccattt cagtaccttc
     4501 tggtaagtga acaacaccag ttacgtcagt agtacggaaa tagaattgtg gacgatagtt
     4561 tgagaagaat ggagtgtgac gtccaccttc gtcttttgat aatacgtata cttctgcttt
     4621 aaattcagta tgtggtgtaa ttgaaccagg agcagctaat acttgaccac gttgtacgtc
     4681 ttcacgagca acaccacgta ataatgcacc aatgttgtca ccagcttcag cgtagtctaa
     4741 taatttacgg aacatttcaa cacctgtaac agttgtttta gatgtgtcat gtaaaccgat
     4801 gatttcaact tcttcaccaa ctttgatttg accacgttca acacggcctg tagcaacagt
     4861 accacgacca gtgattgaga atacgtcctc aactggcatc atgaatggtt tgtcagaatc
     4921 acgttctgga gttggaatgt aagtatctac agcttccatt aattctaaga ttttttcttc
     4981 gtattgagca tcgccttcta aagcttttaa tgctgaacca gcgattacag gtacatcgtc
     5041 acctgggaag tcatattcgc ttaataagtc acgaacttcc atttctacta attctaataa
     5101 ttcttcatcg tcaaccatgt caactttgtt taagaatact actaatgctg gtacaccaac
     5161 gttacgtgat aaaagaatgt gttcacgagt ttgtggcatt ggaccgtcag cagcagatac
     5221 tactaagata ccgccgtcca tttgagcagc accagtgatc atgtttttaa cgtagtcagc
     5281 gtgtcctggg cagtcaacgt gagcgtagtg acgtttgtca gtttggtact caatgtgaga
     5341 agtattgatt gtgataccac gttctttttc ttctggagcg ttgtcaatca tgtcatatga
     5401 ttgtgcaact gagtcaccat tttttgctaa tacagtagcg attgctgctg ttaatgttgt
     5461 tttaccatgg tcaacgtgac cgatagtacc gatattggca tgttctttag aacgatcgaa
     5521 tttttctttt gccattataa aatctctcct attctaatta aaaataatat ttaaatttat
     5581 ctctcatgat agtttctcac catcatgaga aattatttat agcgatgcaa aaagaatttt
     5641 acaattcttt tataatgcat tcaccccaaa aaatcaaact ataaagatga tagcttacat
     5701 ttaagctcac cttgtatttt aacctaggct agctagtcaa aacaagttat attattcacc
     5761 tttatttttc ttgataatat cttcagcgat tgattttgga acttcagcat agtgatcgaa
     5821 gtacatagtg taagtaccgc gaccttgagt gtttgaacgt aatgatgttg cataaccgaa
     5881 catttctgaa agtggtacat aagcattaac aacttgtgca ttaccacgag gttccatacc
     5941 atcaacacgt ccacgacgag atgttacgtc acccatgata tcacccatgt actcttcagg
     6001 catttcaata gttactttca tcattggttc taagattaca ggatcacatt ttttagcagc
     6061 ttctttaagt gctaatgatg cagcaatttt gaaggccatt tcagatgaat cgacatcatg
     6121 gtatgaacca tcatataatt tagctttaac atcaattaaa ggataacctg ctaagacacc
     6181 attttccata gcatctttaa gaccagcttc tactgatgga atgtattcac gaggaactac
     6241 accaccaacg atagcgtttt cgaattcgaa acctgcgcct gtttcgtttg gtgtgaattc
     6301 aatgtgaaca tcaccgtatt gaccacgacc accagattga cgagagaatt taccttgaac
     6361 ttgtgcagat gatttgaatg tttcacgata tgaaaccatt ggagcaccta cgttacattc
     6421 aacgttgaat tctttcttca tacggtctac taagatgtct aagtgaagct cacccatacc
     6481 accgatgata acttgtccag tttcttcgtc agtgtgtgca tggaatgttg ggtcttcttc
     6541 ttgtaattta actaaagctt gagtcatttt atcttggtca gctttagatt ttggctctac
     6601 tgataagtga ataactggct ctgggaattc cattgattcc aagataatgt catttttctc
     6661 accacataaa gtatcaccag tacctgtatc tttaagacct accgcagccg cgatatctcc
     6721 agagtataca gtatcgattt cttgacgtga gttagcgtgc atttgtaata aacgacctac
     6781 acgttcacgt ttacctttag tagagttctt aacgtatgaa ccagatgtca ttgtacctga
     6841 atacacacgg aagaatgtta atttaccaac ataagggtca gtcataactt tgaacgctaa
     6901 tgcagcgaat tcagctgaat cgtctgcttt cgcgattact tcttcttcag ggttgctagc
     6961 acggtgacca ataattggtt taacgtctag tggtgaaggt aagtaatcaa ttacagcgtc
     7021 aagcattaat tgaacacctt tgtttttgaa agctgtacca caaagtactg ggtagaattc
     7081 tacgttagta gtagcttggc ggatagcttc ttttaattca gaaactgaaa tttcttcgtc
     7141 accaagatat ttttccatta attcgtcgct agtttctgca actgcttcga ttaagctagc
     7201 acgagcttct tcagctctat ctaagtggtc ttcaggaatt tcaatttctt caatttcagt
     7261 acctaaatca tttgtatatt tgaaacattt catttcaact aagtcaatga ttgcttcgaa
     7321 ttcgtcttcc gcaccaattg gtaattggat tggagcagcg ttagcttgta aacgatcatg
     7381 taatgtactt acagagtatt cgaagttagc acctaatttg tccattttgt ttacaaatac
     7441 gatacgtgga acaccataag ttgtagcctg acgccaaact gtttcagttt gtggttcaac
     7501 acctgattgt gcatcaagta ctgtaactgc tccgtcaagt acacgtaatg aacgttcaac
     7561 ttctacagtg aagtctacgt gtccaggtgt atcgataatg tttacacggt gaccttccca
     7621 agctgctgtt gttgcagcag atgtgatagt aataccacgg tcttgttctt gctccatcca
     7681 gtccatttgt gaagcacctt cgtgtgtttc accaattttg tggatacggc cagtgtaata
     7741 aagaatacgt tcagtcgtag tcgttttacc agcatcaatg tgagccatga taccgatatt
     7801 acgagttttt tctaatgaaa attctctagc catgtatttt ttctccttcc agtttatatt
     7861 actgaataaa tacgatagat atggcgatgc cctaagcatg aacgcttaat gaaaattcat
     7921 taatatagaa cacactcagg gtaaaagctt ttatcttacc aacggtagtg agcaaatgct
     7981 ttgtttgctt cagccatttt gtgagtgtcc tcacgtttct taacggcacc acctgtatta
     8041 tttgctgcat ctaaaatttc gttagctaaa cgatcttcca tcgttttttc accacgaaga
     8101 cgtgcatagt taactaacca acgtaaacct aaagtagtac gacgctctgg acgaacttct
     8161 actggtactt gatagttaga accacctaca cgacgagctt taacttctaa tactggcata
     8221 atgttgttga ttgcttcttc gaatacttct aatgcatcac gaccactgcg ttgttcaact
     8281 aggtcgaatg ctgaataaag aattctttgt gctgttccac gtttaccatc taacataatt
     8341 ttgttaatta atttagttac taacttagag ttatgaattg gatctggtaa tacgtctctt
     8401 ttaggtactg atcctttacg aggcataatg taaatcctcc cttccttata ttaaatattt
     8461 taagtttaag atttaattaa aaactaaatt cttagttttt aggtttctta gttccgtata
     8521 atgaacgacc ttgtctacgt ccgtcaacac ctgaagtatc aagtgctcca cgtacaatat
     8581 ggtaacgcac acctggtaag tcttttacac gtccaccacg tacaagtaca acactgtgtt
     8641 cttgtaagtt atgtccgata ccagggatgt atgcgttaat ttcgatgttg tttgataaac
     8701 gcacacgtgc atatttacgt aacgctgagt taggtttttt aggtgtcatt gtacctacac
     8761 gagtacatac accacgtttt tgtggtgagt ttaagtcagt aaattttttc tttttactgt
     8821 tgaaaccttt atttaaagct ggagaatctg attttttgat tttgctttgt cttggtttac
     8881 gtactaattg gttaatagtt ggcatgtgat atgtcctcct ctcttcactt tttaatccca
     8941 cacatccagg tggttcattt ttaggttaaa taaaatttag taagtaaaac acttactaat
     9001 tctcatttaa tcaatgctac tattgtcgca ttgacgttaa tacctacatg tttacccaaa
     9061 gcatgtttgc ttttgaaaaa agatacaggt atatttttct gattgatttg gcttaacacg
     9121 cgagtcatta aatatacttc aacgtcttca gcaataatca aagatgtaac ttgatctttc
     9181 tttaacgctt taagcgtttc tttaagacca actacaaaat gttgtttgtt aaagcgtgca
     9241 actttttcct tagacaataa tttatcctcc aaagttctgc ttgcatcaac ctttaatatt
     9301 ttaacattga acaagagaat tcgtcaacat taaatcaaat aatttacata aaaataacaa
     9361 gaggctaaag cattagcctc tgttatatac ttgttattcc gttacttcag tttgagattc
     9421 aacttctgca actggtttag ctgttttttc gtattttacg tcgctataac gtctcatacc
     9481 agtaccagct ggaattaact taccaataat tacgttttct ttaagaccta ataagtcatc
     9541 acgcttacct ttaattgctg catctgtaag aactcttgtt gtttcttgga atgatgctgc
     9601 agataagaaa ctttctgttt caagtgatgc tttagtaata ccaagtaata ctggtttagc
     9661 tgttgcagga cgcttacggt gtttaaatgc ttctctattt gcatctgtaa agttatgaat
     9721 atcaactaat gaacctggta ataacttcgt atcacctgct tcgataattc taactttacg
     9781 taacatttgt cgaaccataa cctcaacgtg tttatcgtcg atttctacac cttgcatacg
     9841 gtaaactttt tgtacttctt ttaataagta gctttcagtc gcgtttaatc cagcaacaga
     9901 taagtaattc ttaggttcaa tagaaccttc agttaatact tcaccacgtt gaactggttg
     9961 accgatttct acaataattc ttgaagtacc tgaagcaagg tatgatcttg tttcattagc
    10021 acctttaaca acaatttctt gttgtctatc ttttgctaat ttaatatctt ctacgacacc
    10081 ttcgatttcc gtaattaccg cttgaccttt agggttacgt gcttcgaaaa tctcttgaat
    10141 acgaggaaga ccttgtgtga tatcgctacc tgctacccca cctgtatgga atgtacgcat
    10201 tgtaagctgt gtacctggtt caccgataga ttgggctgca attgtaccaa ctgcttcacc
    10261 aacttcaact ttttcaccag tagcaaggtt tttaccgtaa catttttcac aaacaccatg
    10321 tcgtgcgtta caagtaaatg ctgagcgaat atacatttgt tcaataccag catctgtaat
    10381 tttcttagca atttcaggtg taattaattc atcaggacga atgattattt catcagtttc
    10441 aggatgacga attgtttctt tagaataacg accttcaata cgttcgataa atggttcaat
    10501 catttctgta ccttctttaa tatcagaaac taataaacct ctatcagtac cacagtcttc
    10561 ttcacgaaca ataacatctt gtgccacgtc aacaagacga cgagtaagat atcctgagtc
    10621 agctgtttta agtgctgtat cggcaagacc tttacgtgca ccgtgagttg agatgaagta
    10681 ttcaagtact gttaaacctt cacggaatga agatgtgatt ggtaattcga taatcttacc
    10741 agatggtgcg gccatcaatc cacgcatacc tgctaactgt gtaaagttag atgcgttacc
    10801 acgggcacct gaatcactca tcatgaagat tgggttagtt ttatcaagtg attgcatcaa
    10861 ttcaccttga atttgatctt ttgcatctgt ccaaatttca acaactgcat tatatctttc
    10921 ttcttcagtg attaaaccac ggttgaattg ttttgtaatt ctgtcgacta atttttcatg
    10981 ctcatcaagt atttgttgct tatcaggtaa tactacgata tcagcaacac ctactgtaat
    11041 accagcttta gatgagaatt tgaatcctaa gtctttcata cggtctaaca tcattgatgt
    11101 atcagtgatg ctaaatctgt tgaatacttc tgcaataata ttacctaaga attttttgtt
    11161 gaaaggttca attaattctt cattttcaaa gtattctttt aatccacctt cacctaaagt
    11221 tgtaggatcg atgaaatatc tgtttggtgt ctttctttct aagttttctt gcgtaggttc
    11281 attaatataa gcaaatgaat ctggaatgat ttcattgaat ataattttac ctactgacgt
    11341 agcaagaatc tttttgtttt gttcttcagt aaatgttgga ttattgaacg aacttgcatg
    11401 tacaccaatt ctagtgtgta aatgtacaaa gccatttgca tatgctttta atacttcatt
    11461 tgtattatta aagattgcgc ctgtatttac tgcatctttt ctttctaaag taaggtaata
    11521 gttaccaagt accatatctt gtgatggtgt aactacaggt ttaccatctt tagggttcaa
    11581 gatgttttgt gctgctaaca tcaacattct tgcttcagct tgtgcctctt ttgataaagg
    11641 aacgtgaacc gccatttggt caccgtcaaa gtcagcatta taagctgttg ttacaagtgg
    11701 atgtagacga atcgcacgac cttcaactaa agttggttca aatgcttgaa taccaagtct
    11761 atgaagtgtt ggtgcacggt taagtaatac aggatgttct ctaattactt cttccaatac
    11821 gtcccaaact tcatcatcca tacgttcgat tttactcttc gcatttttaa tgttagttgc
    11881 aatttcacgt tgaactaatt ctttcattac gaatggttta aatagttcaa gtgccatttc
    11941 ttttggtaaa ccacattggt acattttcaa gcttggacct actgcaataa ctgaacgtcc
    12001 tgaatagtcg acacgtttac caagtaagtt ttgacggaaa cgaccttgtt taccttttaa
    12061 catatgagat aaagatttta atggacggtt acctgggcca gtaactggac gaccacgacg
    12121 accattatca attaaagcgt caacggcttc ttgtaacata cgtttttcgt tttgaacgat
    12181 gataccaggt gcacctaaat ctaataaacg tttcaaacga ttatttcgat taattacacg
    12241 acggtataag tcgtttaagt cacttgttgc aaatcgtcca ccatctaatt gaaccattgg
    12301 acgaatttct ggtgggatga ttggaagtac atctaaaatc atccatgaag gtttgttacc
    12361 tgaattacgg aatgattcaa caacttctaa acgtttaatt gcacgagtaa gtctttgacc
    12421 agtagctgat tccaactcat cgcgtaacaa tttaagttct tcgtcaagat caatctcttc
    12481 aagtaaatct ttaatacctt ctgcacccat ttttgcaacg aattgacctg ggtatttatc
    12541 ataataatct ctgaattcag cttcagataa taaagttttc ttttctaaac cagttggacc
    12601 tggatctaca acaacataag aagcaaagta aataacctct tctaatgctc ttggtgacat
    12661 gtcaagtaat aatcccatac gacttggtat acctttgaaa taccaaatgt gagaaactgg
    12721 agcagcaagt tcaatgtgac ccattctttc acgacgtact ttagatttag ttacttcaac
    12781 tccacatctg tcacagacca tgcctttgta gcgaacacgt ttgtatttac cacaactaca
    12841 ttcccagtct tttgtaggtc cgaaaattct ttcacagaat agaccatctt tttcaggttt
    12901 taatgtacgg tagttgattg tttcaggttt tttaacttca ccaaaagacc aagaacggat
    12961 tttttcaggt gaagccaatc ctattttcat ataatggaaa ttatttacat caatcaagga
    13021 gcctacctcc ttcaatttag attagctgtg ctatttgatt gatttgcatt tatttaaaat
    13081 ggcttaaagt actaaactga atcatcaata aaattcagct tagtatcttt ttgcctgttt
    13141 tgtaaattgc gtattaatca gtaacttctt tttgtgtttc aggagcatca ttttgttgta
    13201 aatctacttt gcgttctaca acatcatcgt catcaacgtc tgtcatttcg atttcattat
    13261 cttgctcatc cataactttt acatctaaac ctaaactttg taattctttc atcaatactc
    13321 ggaatgattc tggaacactt ggtctagaga tgttttcacc tttaacaata gcctcgtatg
    13381 ttttcacacg tcctactgta tcatcggatt tgtaagttaa gatttcttgt aatgtgtatg
    13441 cagcaccata tgcttcaagt gcccatacct ccatctcacc gaaacgttgt ccaccgaatt
    13501 gcgctttacc gccaagtggt tgttgtgtaa caagtgaata tggtcctgtt gaacgcgcat
    13561 gtaatttatc atcaaccatg tgcgcaagtt tcaacatgta cattacacct actgaaatac
    13621 ggttatcgaa tggttcacct gtacgtccat cataaagtac agttttacca tcacgagcca
    13681 taccagcttc ttcaattgtt gaccatacat cgtcatcgtt tgcaccgtca aatactggtg
    13741 atgcaacgtg aataccaaga tttttagcag ccatacctaa gtgtagctct aatacttgtc
    13801 cgatgttcat acgagatggt acaccaagag gatttaacat gatatcgatc ggacgtccat
    13861 ctggtaagta aggcatatct tcttcaggaa caatcttaga aatgacacct ttgttaccat
    13921 gtcgaccaca catcttatca ccaacatgaa ttttacgttt ttgaacgata tatacacgta
    13981 ctaattggtt tacaccaggt gataatgtat cgtcgccttc ttcacgattg aatactttta
    14041 catcaagaac gataccgcca gcgccgtgag gtacacgtaa tgaagtatct ctaacttcac
    14101 gtgctttttc accaaagatt gcatgtaaca atctttcttc ggcagttaac tcagttacac
    14161 ctttaggcgt tactttacca actaaaatat ctccatcttt tacttctgca ccaatataaa
    14221 cgataccacg atcgtctaag ttcttaagtg cactttcaga aacattagga atatctcttg
    14281 tgatttcttc aggtcctaac ttagtatcac gtgcttctga ttcatactct tcaatatgaa
    14341 tagaagtata cacgtcatct ttcacaagtc tttcactcat gataacggca tcctcatagt
    14401 tgtaaccgtc ccaagtcatg aaaccaacta ctacgtttct acctaatgcc atttctccta
    14461 attccataga tggtccatct gctaaaatct cgttatactc aacaacatct ccaactgcaa
    14521 cgattggacg ttggttgtaa catgtacctg agtttgaacg tttaaattta gctaatggat
    14581 agcgatctaa ttcaccttca tgctcaacgc cgttctcttc aactagacga cgtacaagaa
    14641 tttcattaga ttcaacatgt tcaacacgac ctctgtgctt agctgtaata gctgcaccag
    14701 aatcacgtgc tgcaacgtgt tccatacctg ttccaacaaa tggtgcttct ggattcatca
    14761 aaggcactgc ttgacgttgc atgttcgcac ccatcaatgc acggtttgag tcatcgtttt
    14821 ctaagaatgg aatacatgct gtcgctgctg aaacaacttg cttcggcgat acatccatat
    14881 aatccatttt ttctttagcc ataactgtat tgttaccacg gaaacgacat acaacttcat
    14941 catccatgaa acgaccattt tcatctaatt tagagtttgc ttgtgctaca acatagctat
    15001 cttcttcgtc agctgttaaa tagtcaattt gatcagtgat agcatgtgta tctaaatcaa
    15061 ctttacgata tggtgtttca ataaagccga attcatttac acgtgcataa cttgataatg
    15121 agttaatcaa tccaatgttt ggtccctcag gtgtttcaat tggacacata cggccatagt
    15181 gagagtagtg aacgtcacgt acttccattt gagcacgttc acgtgttaaa ccaccaggtc
    15241 ctaatgctga tagacgacgt ttatgcgtta actcagctaa tgggtttgct tggtccatga
    15301 attgtgataa ttgagagcta ccaaagaatt ctttaataga tgcaataaca ggtcgaatat
    15361 taattaattg ttgaggtgtg atagactcag tatcttgaat tgacattctt tcacgtacaa
    15421 ctctttccat tcttgataaa ccgatacgga attggttttg tagtaattca cctacagaac
    15481 gtaaacgacg gttacctaaa tggtcaatat catctgtata tccaataccg cttaataagt
    15541 taaagaagta actcattgaa gcaatgatat ctgctggtgt aatgcattta acttctgagt
    15601 cagggaaagc attaccaatt acagttgtcg tacgaccttc atcatcgtta ggaacatata
    15661 ctttaattga ttgaatttct actggctcgt ctataacgct accatgcaat tcaaacactt
    15721 cgctgtttgc atttgattca agtacatcca tgatttcgtc gattttacga cgatcaagca
    15781 ctgtaccttc ttcaactaca atttcaccag tttcagtatt tacaattggc tcagctaatt
    15841 tttgattaaa taaacgatgt tttaaatgta attttttgtt tgttttataa cgacccacgc
    15901 ttgctaagtc atagcgtttt ggatcaaaga aacgtgaata caatagactt ttagcatttt
    15961 caacagttgg tggttcacct ggacgtaaac gttcatagat ttctaataac gcttgttcag
    16021 tgttttcagt gccgtctttc tctaaagtat tacgtaaata ttcattgtca cctaaaaggt
    16081 caacaatttc ttggtcactt gagaaaccta atgcacgtaa caatactgtt aatggtagtt
    16141 tacgtgttct atcaatacgt acgtatacaa catctttagc atctgtttca tattctaacc
    16201 atgcaccacg gtttggaata attgttgcat catagttttc acgaccattt ttgtcgattt
    16261 tttcattgaa ataaacggat ggtgaacgaa ctaattgaga tacgattaca cgttctgcac
    16321 cattgataac gaacgtacct gtatcagtca ttaatgggaa atcacccata aagacttctt
    16381 gttctttaac ttctcctgtt tctttaatga ttagacgcac ttttacacga agaggtgcag
    16441 cataagtagc gtcacggttt ttagattctt ctaaatcata ttttggttct cctaaacggt
    16501 aatccacaaa ctctaatgac aaattaccag taaaatcttc aattggagaa atgtctctaa
    16561 acatttcgat taaaccttct cttaggaacc actcgtaaga tttagtttga atttctatta
    16621 agtttggtaa ttctaatact tctgaaattc tcgcgtagtt tctacgttta cgatgtcttc
    16681 catattggac aacttgacct gccaaacaga ttcacccctc aaaaattatg tgatccgctt
    16741 ttagctaaat accataagca tattaataaa aagacaaaaa gaaaacggca acactaatga
    16801 tgacaccatt ttcgattcta tatttattct taattaactt atctatttaa cgtaaaagta
    16861 cacacttatt gaacataaat ttttacatta tataactata acagaatatc catttcattt
    16921 caagctttta tacttctcag aatgtaatat cctttatctt tatttaccac ttctacattt
    16981 ccaaaaagtt cattcattct tttctttgca gatggcatac cttgcttctt ctgaattaca
    17041 acgaatagtt caccgttcga gtctaatcta tgtaatgctt gctcgaatat acgatgcacg
    17101 gtttctttcc ctgctcttat tggtggattg gttaaaataa aatcaaaact tttgtcttcc
    17161 acagcagaca aagcatcact ttcctttacg atcacattat caataccatt taattttttg
    17221 tttttttcaa ctaaggctag cgctctgtga ttaacatcta gcattgtaat tgaatgatgt
    17281 ggtgatactt tagcaatcat caaaccaatt ggtccgtaac cacaaccaac atcggcaatt
    17341 cgcttacttg gaccaggtgg atgcgctttt aaaaaagttt gaacaagaac atctgaacca
    17401 taatctactt tatctttcga aaacactccg ttatcagtta ttaaatcaat tttatgatgg
    17461 tttaattgat attgaatacg ttgttcattg ctaattacac ttggatcttc atcgtaataa
    17521 tgactcatta ttccacctca tcttcacttt gtaactcgtc attataatta taacatttca
    17581 ctcagtcttt aaatgaataa aaccccgtta tcaaaataac ggggtt
//
LOCUS       DAHHSC010000037        17525 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.037, whole genome shotgun sequence.
ACCESSION   DAHHSC010000037 DAHHSC010000000
VERSION     DAHHSC010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 17525)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17525)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17525
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.037"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            177..947
                     /locus_tag="NBI57_000948"
     CDS             177..947
                     /locus_tag="NBI57_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HCD1821912.1"
                     /translation="MFKKLINKKNTINNYNEELDSSNIPEHIAIIMDGNGRWAKKRKM
                     PRIKGHYEGMQTIKKITRIASDIGVKYLTLYAFSTENWSRPESEVNYIMNLPVNFLKT
                     FLPELIEKNVKVETIGFTDKLPKSTIEAINNAKEKTANNTGLKLIFAINYGGRAELVH
                     SIKNMFDELHQQGLNSDIIDETYINNHLMTKDYPDPELLIRTSGEQRISNFLIWQVSY
                     SEFIFNQKLWPDFDEDELIKCIKIYQSRQRRFGGLSEE"
     gene            954..1736
                     /locus_tag="NBI57_000949"
     CDS             954..1736
                     /locus_tag="NBI57_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HCD1821913.1"
                     /translation="MKVRTLTAIIALIVFLPILLKGGLVLMIFANILALIALKELLNM
                     NMIKFVSVPGLISAVGLIIIMLPQHAGPWVQVIQLKSLIAMSFIVLSYTVLSKNRFSF
                     MDAAFCLMSVAYVGIGFMFFYETRSEGLHYILYAFLIVWLTDTGAYLFGKMMGKHKLW
                     PVISPNKTIEGFIGGLFCSLIVPLAMLYFVDFNMNVWILLGVTLILSLFGQLGDLVES
                     GFKRHFGVKDSGRILPGHGGILDRFDSFMFVLPLLNILLIQS"
     gene            1948..3234
                     /gene="rseP"
                     /locus_tag="NBI57_000950"
     CDS             1948..3234
                     /gene="rseP"
                     /locus_tag="NBI57_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000121122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HCD1821914.1"
                     /translation="MSYLVTIIAFIIVFGVLVTVHEYGHMFFAKRAGIMCPEFAIGMG
                     PKIFSFRKNETLYTIRLLPVGGYVRMAGDGLEEPPVEPGMNVKIKLNEENEITHIILD
                     DHHKFQQIEAIEVKKCDFKDDLFIEGITAYDNERHHFKIARKSFFVENGSLVQIAPRD
                     RQFAHKKPWPKFLTLFAGPLFNFILALVLFIGLAYYQGTPTSTVEQVADKYPAQQAGL
                     QKGDKIVQIGKYKISEFDDVDKALDKVKDNKTTVKFERDGKTKSVELTPKKTERKLTK
                     VSSETKYVLGFQPASEHTLFKPIVYGFESFLKGSTLIFTAVVGMLASIFTGGFSFDML
                     NGPVGIYHNVDSVVKAGIISLIGYTALLSVNLGIMNLIPIPALDGGRILFVIYEAIFR
                     KPVNKKAETTIIAIGAIFMVVIMILVTWNDIRRYFL"
     gene            3254..4957
                     /locus_tag="NBI57_000951"
     CDS             3254..4957
                     /locus_tag="NBI57_000951"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HCD1821915.1"
                     /translation="MKQSKVFIPTMRDVPSEAEAQSHRLLLKSGLIKQSTSGIYSYLP
                     LATRVLNNITAIVRQEMERIDSVEILMPALQQAELWEESGRWGAYGPELMRLQDRHGR
                     QFALGPTHEELVTSIVRNELKSYKQLPMTLFQIQSKFRDEKRPRFGLLRGREFIMKDA
                     YSFHADEASLDQTYQDMYQAYSRIFERVGINARPVVADSGAIGGSHTHEFMALSAIGE
                     DTIVYSKESDYAANIEKAEVVYEPNHKHSTVQPLEKIETPNVKTAQELADFLGRPVDE
                     IVKTMIFKVDGEYIMVLVRGHHEINDIKLKSYFGTDNIELATQDEIVNLVGANPGSLG
                     PVIDKEIKIYADNFVQDLNNLVVGANEDGYHLINVNVGRDFNVDEYGDFRFILEGEKL
                     SDGSGVAHFAEGIEVGQVFKLGTKYSESMNATFLDNQGKAQPLIMGCYGIGISRTLSA
                     IVEQNHDDNGIVWPKSVTPFDLHLISINPKKDDQRELADALYAEFNTKFDVLYDDRQE
                     RAGVKFNDADLIGLPLRIVVGKRASEGIVEVKERLTGDSEEVHIDDLMTVITNKYDNL
                     K"
     gene            5221..9531
                     /locus_tag="NBI57_000952"
     CDS             5221..9531
                     /locus_tag="NBI57_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HCD1821916.1"
                     /translation="MTEQQKFKVLADQIKISNQLDAEILNSGELTRIDVSNKNRTWEF
                     HITLPQFLAHEDYLLFINAIEQEFKDIANVTCRFTVTNGTNQDEHAIKYFGHCIDQTA
                     LSPKVKGQLKQKKLIMSGKVLKVMVSNDIERNHFDKACNGSLIKAFRNCGFDIDKIIF
                     ETNDNDQEQNLASLEAHIQEEDEQSARLATEKLEKMKAEKAKQQDNNESAVDKCQIGK
                     PIQIENIKPIESIIEEEFKVAIEGVIFDINLKELKSGRHIVEIKVTDYTDSLVLKMFT
                     RKNKDDLEHFKALSVGKWVRAQGRIEEDTFIRDLVMMMSDIEEIKKATKKDKAEEKRV
                     EFHLHTAMSQMDGIPNIGAYVKQAADWGHPAIAVTDHNVVQAFPDAHAAAEKHGIKMI
                     YGMEGMLVDDGVPIAYKPQDVVLKDATYVVFDVETTGLSNQYDKIIELAAVKVHNGEI
                     IDKFERFSNPHERLSETIINLTHITDDMLVDAPEIEEVLTEFKEWVGDAIFVAHNASF
                     DMGFIDTGYERLGFGPSTNGVIDTLELSRTINTEYGKHGLNFLAKKYGVELTQHHRAI
                     YDTEATAYIFIKMVQQMKELGVLNHNEINKKLSNEDAYKRARPSHVTLIVQNQQGLKN
                     LFKIVSASLVKYFYRTPRIPRSLLDEYREGLLVGTACDEGELFTAVMQKDQSQVEKIA
                     KYYDFIEIQPPALYQDLIDRELIRDTETLHEIYQRLIHAGDTAGIPVIATGNAHYLFE
                     HDGIARKILIASQPGNPLNRSTLPEAHFRTTDEMLNEFHFLGEEKAHEIVVKNTNELA
                     DRIERVVPIKDELYTPRMEGANEEIRELSYTNARKLYGEDLPQIVIDRLEKELKSIIG
                     NGFAVIYLISQRLVKKSLDDGYLVGSRGSVGSSFVATMTEITEVNPLPPHYICPNCKT
                     SEFFNDGSVGSGFDLPDKTCETCGAPLIKEGQDIPFETFLGFKGDKVPDIDLNFSGEY
                     QPNAHNYTKVLFGEDKVFRAGTIGTVAEKTAFGYVKGYLNDQGIHKRGAEIDRLVKGC
                     TGVKRTTGQHPGGIIVVPDYMDIYDFTPIQYPADDQNSAWMTTHFDFHSIHDNVLKLD
                     ILGHDDPTMIRMLQDLSGIDPKTIPVDDKEVMQIFSTPESLGVTEDEILCKTGTFGVP
                     EFGTGFVRQMLEDTKPTTFSELVQISGLSHGTDVWLGNAQELIKTGICDLSSVIGCRD
                     DIMVYLMYAGLEPSMAFKIMESVRKGKGLTEEMIETMKENEVPDWYLDSCLKIKYMFP
                     KAHAAAYVLMAVRIAYFKVHHPLYYYASYFTIRASDFDLITMIKDKTSIRNTVKDMYS
                     RYMDLGKKEKDVLTVLEIMNEMAHRGYRMQPISLEKSQAFEFIIEGDTLIPPFISVPG
                     LGENVAKRIVEARDDGPFLSKEDLNKKAGLSQKIIEYLDELGSLPNLPDKAQLSIFDM
                     "
     gene            9821..10288
                     /gene="rimP"
                     /locus_tag="NBI57_000953"
     CDS             9821..10288
                     /gene="rimP"
                     /locus_tag="NBI57_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HCD1821917.1"
                     /translation="MSKITEQVEVIVQPIMEDLNFELVDVEYVKEGRDHFLRISIDKE
                     GGVDLNDCTLASEKISEAMDANDPIPEMYYLDVASPGAERPIKKEQDFQNAITKPVFV
                     SLYVPIEGEKEWLGILQEVNNETIVVQVKIKARTKDIEIPRDKIAKARHAVMI"
     gene            10309..11484
                     /gene="nusA"
                     /locus_tag="NBI57_000954"
     CDS             10309..11484
                     /gene="nusA"
                     /locus_tag="NBI57_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HCD1821918.1"
                     /translation="MSSNELLLATEYLEKEKKIPRVVLIDAIEAALITAYKKNYDSAR
                     NVRVELNMDQGTFKVIARKDVVEEVFDDRDEVDLSTALVKNPAYEIGDIYEEDVTPKD
                     FGRVGAQAAKQAVMQRLRDAEREILFEEFIDKEEDILTGIIDRVDHRYVYVNLGRIEA
                     VLSEAERSPNEKYIPNERIKVYVNKVEQTTKGPQIYVSRSHPGLLKRLFEQEVPEIYD
                     GTVIVKSVAREAGDRSKISVFSENNDIDAVGACVGAKGARVEAVVEELGGEKIDIVQW
                     NEDPKVFVKNALSPSQVLEVIVDETNQSTVVVVPDYQLSLAIGKRGQNARLAAKLTGW
                     KIDIKSETDAREVGIYPVVEAEKVTEEDIALEDADTTESTEEVNDVSVETNVEKESE"
     gene            11505..11789
                     /locus_tag="NBI57_000955"
     CDS             11505..11789
                     /locus_tag="NBI57_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HCD1821919.1"
                     /translation="MKKKKIPMRKCILSNEMHPKKDMIRVVVNKEGEIFADVTGKKQG
                     RGAYVSKDVAMVEKAQQKEILEKYFKASKEQLDPVYKEIIRLIYREEIPK"
     gene            11786..12103
                     /locus_tag="NBI57_000956"
     CDS             11786..12103
                     /locus_tag="NBI57_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ family RNA-binding protein"
                     /protein_id="HCD1821920.1"
                     /translation="MSIDQILNFLGLAMRAGKVKTGESVIVNEIKKGNLKLVIVANDA
                     SDNTAKLITDKCKSYKVPFRKFGNRNELGIALGKGERVNVGITDPGFAKKLLSMIDEY
                     HKE"
     gene            12108..14225
                     /gene="infB"
                     /locus_tag="NBI57_000957"
     CDS             12108..14225
                     /gene="infB"
                     /locus_tag="NBI57_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HCD1821921.1"
                     /translation="MSKQRIYEYAKELNLKSKEIIDELKSMNIEVSNHMQALEDDQIK
                     ALDKKFKKEQKNDNKQSTQNNHQKSNNQNQNKGQQKDNKKNQQQNNKGNKGNKKNNRN
                     NKKNNKNNKPQNQPAAPKEIPSKVTYQEGITVGEFADKLNVESSEIIKKLFLLGIVAN
                     INQSLNQETIELIADDYGVEVEEEVVINEEDLSIYFEDEKDDPEAIERPAVVTIMGHV
                     DHGKTTLLDSIRHTKVTAGEAGGITQHIGAYQIENDGKKITFLDTPGHAAFTTMRARG
                     AQVTDITILVVAADDGVMPQTIEAINHAKEAEVPIIVAVNKIDKPTSNPDRVMQELTE
                     YGLIPEDWGGETIFVPLSALSGDGIDDLLEMIGLVAEVQELKANPKNRAVGTVIEAEL
                     DKSRGPSASLLVQNGTLNVGDAIVVGNTYGRIRAMVNDLGQRIKTAGPSTPVEITGIN
                     DVPQAGDRFVVFSDEKQARRIGESRHEASIVQQRQESKNVSLDNLFEQMKQGEMKDLN
                     VIIKGDVQGSVEALAASLMKIDVEGVNVRIIHTAVGAINESDVTLANASNGIIIGFNV
                     RPDSGAKRAAEAENVDMRLHRVIYNVIEEIESAMKGLLDPEFEEQVIGQAEVRQTFKV
                     SKVGTIAGCYVTEGKITRNAGVRIIRDGIVQYEGELDTLKRFKDDAKEVAKGYECGIT
                     IENYNDLKEGDVIEAFEMVEIKR"
     gene            14611..14961
                     /gene="rbfA"
                     /locus_tag="NBI57_000958"
     CDS             14611..14961
                     /gene="rbfA"
                     /locus_tag="NBI57_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HCD1821922.1"
                     /translation="MSSMRAERVGEQMKKELMDIINNKVKDPRVGFITITDVVLTNDL
                     SQAKVFLTVLGNDKEVENTFKALDKAKGFIKSELGSRMRLRIMPELMYEYDQSIEYGN
                     KIERMIQDLHKQDR"
     gene            15130..16047
                     /gene="truB"
                     /locus_tag="NBI57_000959"
     CDS             15130..16047
                     /gene="truB"
                     /locus_tag="NBI57_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HCD1821923.1"
                     /translation="MYNGILPVYKERGLTSHDVVFKLRKILKTKKIGHTGTLDPEVAG
                     VLPVCIGNATRVSDYVMDMGKAYEATVSIGRSTTTEDQTGDTLETKGVHSADFNKDDI
                     DRLLENFKGVIEQIPPMYSSVKVNGKKLYEYARNNETVERPKRKVNIKDIGRISELDF
                     KENECHFKIRVICGKGTYIRTLATDIGVKLGFPAHMSKLTRIESGGFVLKDSLTLEQI
                     KELHEQDSLQNKLFPLEYGLKGLPSIKIKDSHIKKRILNGQKFNKNEFDNKIKDQIVF
                     IDDDSEKVLAIYMVHPTKESEIKPKKVFN"
     gene            16062..17033
                     /gene="ribF"
                     /locus_tag="NBI57_000960"
     CDS             16062..17033
                     /gene="ribF"
                     /locus_tag="NBI57_000960"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HCD1821924.1"
                     /translation="MKVIEVTHPIQSKQYITEDVAMAFGFFDGMHKGHDKVFDILNEI
                     AEARSLKKTVMTFDPHPSVVLNPKRKRTTYLTPLSDKIEKISQHDIDYCIVVNFSSRF
                     ANVSVEDFVENYIIKNNVKEVIAGFDFTFGKFGKGNMTVLQEYDAFNTTIVSKQEIEN
                     EKISTTSIRQDLINGELQKANDALGYIYSIKGTVVQGEKRGRTIGFPTANIQPSDDYL
                     LPRKGVYAVSIEIGTENKLYRGVANIGVKPTFHDPNKAEVVIEVNIFDFEDNIYGERV
                     TVNWHHFLRPEIKFDGIDPLVKQMNDDKSRAKYLLAVDFGDEVAYNI"
     gene            17148..17417
                     /gene="rpsO"
                     /locus_tag="NBI57_000961"
     CDS             17148..17417
                     /gene="rpsO"
                     /locus_tag="NBI57_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011275926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HCD1821925.1"
                     /translation="MAISQERKNEIIKEYRVHETDTGSPEVQIAVLTAEINAVNEHLR
                     THKKDHHSRRGLLKMVGRRRHLLNYLRSKDIQRYRELIKSLGIRR"
BASE COUNT         6354 a         2364 c         3446 g         5361 t
ORIGIN      
        1 gtaattttta acctcagtat gtatgtcaca tttctggtgt aaactgtacc gagtcagact
       61 ttggtacagt ttttttattt gcttattcaa tgcattaaat gagtatgata aaatgataat
      121 gattgtttag taacttatat tatatgacag agatgatcag gctcggagga aagaccatgt
      181 ttaaaaagct aataaataaa aagaacacta taaataatta taatgaagaa ttagactcgt
      241 ctaatatacc tgaacatatc gctattatta tggatggtaa tgggcgatgg gctaagaagc
      301 gaaaaatgcc tagaattaaa ggccattacg aaggtatgca aacaataaaa aaaattacta
      361 ggatagctag tgatattggt gttaagtact taactttata tgccttttcc actgaaaatt
      421 ggtcaagacc tgaaagtgaa gtaaattata ttatgaattt gcctgtcaat ttcttaaaga
      481 cattcttacc ggaactaatt gaaaaaaatg tcaaagttga aacaattgga tttactgata
      541 agttgccaaa atcaacgata gaagcaatta ataatgctaa agaaaagaca gctaataata
      601 ccggcttaaa attaatattt gcaattaatt atggtggcag agcagaactt gttcatagta
      661 ttaaaaatat gtttgacgag cttcatcaac aaggtttaaa tagtgatatc atagatgaaa
      721 catatataaa caatcattta atgacaaaag actatcctga tccagagttg ttaattcgta
      781 cttcaggaga acaaagaata agtaatttct tgatttggca agtttcgtat agtgaattta
      841 tctttaatca aaaattatgg cctgactttg acgaagatga attaattaaa tgtataaaaa
      901 tttatcagtc acgtcaaaga cgctttggcg gattgagtga ggagtagtat agtatgaaag
      961 ttagaacgct gacagctatt attgccttaa tcgtattctt gcctatcttg ttaaaaggcg
     1021 gccttgtgtt aatgatattt gctaatatat tagcattgat tgcattaaaa gaattgttga
     1081 atatgaatat gattaaattt gtttcagttc ctggtttaat tagtgcagtt ggtcttatca
     1141 tcattatgtt gccacaacat gcagggccat gggtacaagt aattcaatta aaaagtttaa
     1201 ttgcaatgag ctttattgta ttaagttata ctgtcttatc taaaaacaga tttagtttta
     1261 tggatgctgc attttgctta atgtctgtgg cttatgtagg cattggtttt atgttctttt
     1321 atgaaacgag atcagaagga ttacattaca tattatatgc ctttttaatt gtttggctta
     1381 cagatacagg ggcttactta tttggtaaaa tgatgggcaa acataagctt tggccagtaa
     1441 taagtccgaa taagacaatc gaaggattca taggtggctt gttctgtagt ttgatagtgc
     1501 ctcttgcaat gttatatttt gtagatttca atatgaatgt atggatatta cttggagtga
     1561 cattgatttt aagtttattt ggtcaattag gtgatttagt ggaatcagga tttaagcgtc
     1621 atttcggcgt taaagactca ggtcgaatac tacctggaca cggtggtatt ttagaccgat
     1681 ttgacagctt tatgtttgtg ttaccattat taaatatttt attaatacaa tcttaatgct
     1741 gagaacaaat caataaacgt aaagaggagt tgctgagata atttaatgaa tctcagaact
     1801 ccttttgaaa attatacgca atattaactt tgaaaattag acgttatatt ttgtgatttg
     1861 tcagtatcat attataatga cttatgttac gtatacagca atcaatttta aaataaaaga
     1921 aatttataaa caatcgaggt gtagcgagtg agctatttag ttacaataat tgcatttatt
     1981 attgtttttg gtgtactagt aactgttcat gaatatggac atatgttttt tgcgaaaaga
     2041 gcaggcatta tgtgtccaga atttgcgatc ggtatggggc caaaaatttt tagttttaga
     2101 aaaaatgaaa cactttacac tattaggtta ttgcctgttg gtggatatgt tcgtatggca
     2161 ggagatggct tagaagagcc accagtcgag cccggtatga acgttaaaat taaactgaat
     2221 gaagaaaatg aaataacaca tatcatatta gatgatcatc ataagtttca acaaattgaa
     2281 gcgatcgaag ttaaaaaatg tgattttaag gatgacttat tcatagaagg tatcactgct
     2341 tatgataatg aaagacatca ttttaaaatt gctagaaagt ctttctttgt tgaaaatggt
     2401 agcttagttc aaattgctcc gagagacaga caatttgcac ataaaaagcc atggccgaaa
     2461 tttttaacat tatttgcggg accgttattt aactttatat tagctttagt cctatttatt
     2521 ggtcttgcat attatcaagg tacacctacg tctactgtag aacaagtcgc agataagtat
     2581 ccagctcaac aagcaggatt acaaaaaggt gataaaattg ttcaaattgg caaatataaa
     2641 atatctgaat ttgatgatgt tgataaggcg ttagataaag ttaaagataa taagacgact
     2701 gttaaatttg aacgtgatgg taaaacgaag tcagttgaat taacacctaa aaagactgaa
     2761 agaaaactga ctaaagtaag ttcagagacg aagtatgttc tcggattcca accagcgagt
     2821 gaacatacac ttttcaaacc aattgtatat ggatttgaaa gctttttgaa aggtagtaca
     2881 ctaattttta cagctgtagt cggtatgttg gctagtatat ttacgggcgg attctcattt
     2941 gatatgttaa atggtccggt tggtatttat cataacgtcg actcagttgt taaagcgggt
     3001 atcattagct taattggtta cactgcgtta ttaagtgtaa acttaggtat tatgaattta
     3061 attcctattc ctgcactaga cggtggtcgt attttatttg ttatatatga agcgattttc
     3121 agaaaaccag ttaataaaaa agcggaaaca acgattattg ctattggtgc cattttcatg
     3181 gtcgttatta tgatattagt aacgtggaat gatattcgac gatatttctt ataatttagg
     3241 aggataaata attatgaagc aatccaaagt ttttatacca acgatgcgtg atgtgccatc
     3301 agaagcagaa gcacaaagtc atcgtttatt attgaaatcg ggtttgataa aacaaagtac
     3361 aagtgggatt tatagttatt taccgctagc aacacgtgtg ttaaataata ttactgcaat
     3421 tgtgcgacaa gaaatggaac gtatcgattc tgttgaaatt ttaatgccag cgttacaaca
     3481 agctgaatta tgggaagaat caggacgttg gggtgcatat ggcccagaat taatgcgttt
     3541 acaagataga catggaagac aatttgcatt aggtccaaca catgaagaat tagttacatc
     3601 aatagtaaga aatgaattga aatcatacaa acaattaccg atgacattat tccaaattca
     3661 atctaaattc cgtgatgaaa agagaccacg ttttggttta cttcgtgggc gtgaatttat
     3721 tatgaaagat gcgtattcat tccatgctga cgaggcatca ttagatcaaa cgtatcaaga
     3781 tatgtatcaa gcgtatagcc gtatttttga gagagttggc attaatgcta gaccagttgt
     3841 agcagattca ggtgctatag gcggtagcca tacacatgaa tttatggcat taagtgctat
     3901 cggtgaggat acaatcgttt acagtaaaga aagtgactat gctgctaata tcgaaaaagc
     3961 agaagtcgtt tacgaaccaa atcataagca ttctactgtg caacctttag aaaaaattga
     4021 aacaccaaat gttaagactg cacaagaatt ggcagacttc ttaggtagac cagtagatga
     4081 aatcgttaaa acgatgattt tcaaagttga tggcgaatat attatggttt tagtgcgtgg
     4141 ccatcatgaa attaatgaca ttaaattaaa atcttatttc ggcacagata atattgaatt
     4201 agcaacacaa gacgaaattg ttaatttagt tggtgcaaat ccgggttcac taggtcctgt
     4261 tattgataaa gaaatcaaaa tttatgcaga taattttgtg caagatttaa ataatttagt
     4321 tgtcggtgct aacgaagatg gctatcactt aattaatgta aatgtaggta gagacttcaa
     4381 cgttgatgaa tatggcgatt tccgttttat tttagaaggc gaaaagttaa gtgatggttc
     4441 aggcgttgca cattttgctg aaggtattga agttggtcaa gtattcaaat tgggtactaa
     4501 gtattcagaa tcaatgaatg ctacattctt agataaccaa ggaaaagctc aacctttaat
     4561 tatgggctgt tacggtattg gaatttctag aacgctaagt gcgattgttg aacaaaatca
     4621 cgatgataat ggaattgttt ggcctaaatc agttactcca tttgatttac atttaatttc
     4681 tattaatcct aagaaagatg atcaacgaga gctagcagat gcactatatg ctgaatttaa
     4741 tactaaattt gatgtgttgt acgatgatcg tcaggaacgt gcaggtgtca aatttaatga
     4801 tgccgattta attggtttac cactgcgaat tgttgttggt aaacgtgcat cggaaggtat
     4861 tgtagaagtt aaagaacgtt taacaggtga tagcgaagaa gttcacattg atgacttaat
     4921 gactgtcatt acaaataaat atgataactt aaaataatta agatcgaatg aattataaga
     4981 gtaggaaaaa gctgaaagaa atctgatgct tatgtcctgc tcttattatt tttgatataa
     5041 tgattattcg atgaaaaatg actgaagaca tagtataatt aaagataaat ttgttttaac
     5101 aatattatga ttagccaaat ataaagcatt taattttcta tcattactat gctcacataa
     5161 tctaaatatt gttcgaacac gtaaaagtaa tttctattta aggtggtaat tgtcttggca
     5221 atgacagagc aacaaaaatt taaagtgctt gctgatcaaa ttaaaatttc aaatcaatta
     5281 gatgctgaaa ttttaaattc aggtgaactg acacgtatag atgtttctaa caaaaacaga
     5341 acatgggaat ttcatattac attaccacaa ttcttagctc atgaagatta tttattattt
     5401 ataaatgcaa tagagcaaga gtttaaagat atcgccaacg ttacatgtcg ttttacggta
     5461 acaaatggca cgaatcaaga tgaacatgca attaaatact ttgggcactg tattgaccaa
     5521 acagctttat ctccaaaagt taaaggtcaa ttgaaacaga aaaagcttat tatgtctgga
     5581 aaagtattaa aagtaatggt atcaaatgac attgaacgta atcattttga taaggcatgt
     5641 aatggaagtc ttatcaaagc gtttagaaat tgtggttttg atatcgataa aatcatattc
     5701 gaaacaaatg ataatgatca agaacaaaac ttagcttctt tagaagcaca tattcaagaa
     5761 gaagacgaac aaagtgcacg attggcaaca gagaaacttg aaaaaatgaa agctgaaaaa
     5821 gcgaaacaac aagataacaa cgaaagtgct gtcgataagt gtcaaattgg taagccgatt
     5881 caaattgaaa atattaaacc aattgaatct attattgagg aagagtttaa agttgcaata
     5941 gagggtgtca tttttgatat aaacttaaaa gaacttaaaa gtggtcgtca tatcgtagaa
     6001 attaaagtga ctgactatac ggactcatta gttttaaaaa tgtttactcg taaaaacaaa
     6061 gatgatttag aacattttaa agcgctaagt gtaggtaaat gggttagggc tcaaggtcgt
     6121 attgaagaag atacatttat tagagattta gttatgatga tgtctgatat tgaagagatt
     6181 aaaaaagcga caaaaaaaga taaggctgaa gaaaagcgtg tagaattcca cttgcatact
     6241 gcaatgagcc aaatggatgg tatacccaat attggtgcgt atgttaaaca ggcagcagac
     6301 tggggacatc cagccattgc ggttacagac cataatgttg tgcaagcatt tccagatgct
     6361 cacgcagcag cggaaaaaca tggcattaaa atgatatacg gtatggaagg tatgttagtt
     6421 gatgatggtg ttccgattgc atacaaacca caagatgtcg tattaaaaga tgctacttat
     6481 gttgtgttcg acgttgagac aactggttta tcaaatcagt atgataaaat catcgagctt
     6541 gcagctgtga aagttcataa cggtgaaatc atcgataagt ttgaaaggtt tagtaatccg
     6601 catgaacgat tatcggaaac gattatcaat ttgacgcata ttactgatga tatgttagta
     6661 gatgcccctg agattgaaga agtacttaca gagtttaaag aatgggttgg cgatgcgata
     6721 ttcgtagcgc ataatgcttc gtttgatatg ggctttatcg atacgggata tgaacgtctt
     6781 gggtttggac catcaacgaa tggtgttatc gatactttag aattatctcg tacgattaat
     6841 actgaatatg gtaaacatgg tttgaatttc ttagctaaaa aatatggcgt agaattaacg
     6901 caacatcacc gtgccattta tgatacagaa gcaacagctt acattttcat aaaaatggtt
     6961 caacaaatga aagaattagg tgtattaaat cataacgaaa tcaacaaaaa actcagtaat
     7021 gaagatgcat ataaacgtgc aagacctagc catgtcacat taattgtaca aaaccaacaa
     7081 ggtcttaaaa atctatttaa aattgtaagt gcatcattgg tgaagtattt ctaccgtaca
     7141 cctcgaattc cacgttcatt gttagatgaa tatcgtgagg gattattggt aggtacagcg
     7201 tgtgatgaag gtgaattatt tacggcagtt atgcagaagg accagagcca agttgaaaaa
     7261 attgccaaat attatgattt tattgaaatt caaccaccgg cactttatca agatttaatt
     7321 gatagagagc ttattagaga tactgaaaca ttacatgaaa tttatcaacg tttaatacat
     7381 gcaggtgaca cagcgggtat acctgttatt gcgacaggaa atgcacacta tttgtttgaa
     7441 catgatggta tcgcacgtaa aattttaata gcatcacaac ccggcaatcc acttaatcgc
     7501 tcaactttac cggaagcaca ttttagaact acagatgaaa tgttaaacga gtttcatttt
     7561 ttaggtgaag aaaaagcgca tgaaattgtt gtgaaaaata caaacgaatt agcagatcga
     7621 attgaacgtg ttgttcctat taaagatgaa ttatacacac cgcgtatgga aggtgctaac
     7681 gaagaaatta gagaactaag ttatacaaat gcgcgtaaac tgtatggtga agacctgcct
     7741 caaatcgtaa ttgatcgatt agaaaaagaa ttaaaaagta ttatcggtaa tggatttgcg
     7801 gtaatttact taatttcgca acgtttagtt aaaaaatcat tagatgatgg atacttagtt
     7861 ggttcccgtg gttcagtagg ttctagtttt gtagcgacaa tgactgagat tactgaagta
     7921 aacccgttac cgccacacta tatttgtccg aactgtaaaa cgagtgaatt tttcaatgat
     7981 ggttcagtag gatcaggatt cgatttacct gataagacgt gtgaaacttg tggagcgcca
     8041 cttattaaag aaggacaaga tattccgttt gaaacatttt taggatttaa gggagataaa
     8101 gttcctgata tcgacttaaa ctttagtggt gaatatcaac cgaatgccca taactacaca
     8161 aaagtattat ttggtgagga taaagtattc cgtgcaggta caattggtac cgttgctgaa
     8221 aagactgctt ttggttatgt taaaggttat ttgaatgatc aaggcatcca caaaagaggc
     8281 gctgaaatag atcgactcgt taaaggatgt acaggtgtta aacgtacaac tggacagcat
     8341 ccaggaggta ttattgtagt acctgattac atggatattt atgattttac gccgatacaa
     8401 tatcctgccg atgatcaaaa ttcagcatgg atgacgacac attttgattt ccattctatt
     8461 catgataatg tattaaaact tgatatactt ggacacgatg atccaacaat gattcgtatg
     8521 cttcaagatt tatcgggcat tgatccaaaa acgatacctg tagatgacaa agaagtcatg
     8581 caaatattta gtacacctga aagtttaggt gttactgaag atgaaatttt atgtaaaaca
     8641 ggtacgtttg gggttccaga attcggtaca gggttcgtgc gtcaaatgtt agaagataca
     8701 aagccaacaa cattttctga attagttcaa atctcaggat tatctcatgg tacagatgtg
     8761 tggttaggca atgctcaaga attaattaaa accggtatat gtgatttatc aagtgtaatt
     8821 ggttgtcgtg atgatatcat ggtttattta atgtatgctg gtttagaacc atcaatggct
     8881 tttaaaataa tggagtcagt acgtaaaggt aaaggtttaa ctgaagaaat gattgaaacg
     8941 atgaaagaaa atgaagtgcc ggattggtat ttagattcat gtcttaaaat taagtacatg
     9001 ttccctaaag cccatgcagc agcatacgtt ttaatggcag tacgtatcgc atatttcaaa
     9061 gtacatcatc cactttatta ctatgcatct tactttacaa ttcgtgcgtc agattttgat
     9121 ttaatcacga tgattaaaga taaaacaagc attcgaaata ctgtaaaaga catgtattct
     9181 cgctatatgg atctaggtaa aaaagaaaaa gacgtattaa cagtcttgga aattatgaat
     9241 gaaatggcgc atcgaggtta tcgaatgcaa ccgattagtt tagaaaagag tcaggcgttc
     9301 gaatttatca ttgaaggcga tacacttatt ccgccgttca tatcagtgcc tgggcttggc
     9361 gaaaacgttg cgaaacgaat tgttgaagct cgtgacgatg gcccattttt atcaaaagaa
     9421 gatttaaaca aaaaagctgg attatctcag aaaattattg agtatttaga tgagttaggc
     9481 tcattaccga atttaccaga taaagctcaa ctttcgatat ttgatatgta aaatgaaata
     9541 atcaaggtat ttatttaatg cgtatggcgt agttaaagaa atacaaaatt ggtgctggac
     9601 ataaaattat gcctgtattt cttttcaacg tcttacgagt ctattcgaat gtaatggtga
     9661 aataaatgaa caaactttta caagaatctc tgattaatag tgaagtcatt tgtttcaagc
     9721 ataaacttat gctataatta agttgcttaa aaattagtga actcaggcag aagagtggga
     9781 gattcccgct cttttctatt tgccaaaaag ggaggcctgt atgagtaaaa ttacagaaca
     9841 agtagaagtg attgttcaac caattatgga agacttgaat tttgaacttg tagacgttga
     9901 atatgtcaaa gagggtagag atcattttct tagaatctct attgataaag aaggtggcgt
     9961 agatttaaat gattgtacgc tagcttctga aaaaataagt gaagctatgg atgcaaatga
    10021 tcctattcct gaaatgtatt atttagacgt agcgtcacct ggtgcagaac gtccaattaa
    10081 aaaagaacaa gatttccaaa atgcaataac taaacctgta tttgtttctt tatatgtacc
    10141 aattgaaggt gaaaaggaat ggttaggcat tttacaagaa gtcaataatg aaacaattgt
    10201 agtacaagtt aaaatcaaag caagaacgaa agatatagag ataccgagag acaaaatagc
    10261 aaaagcacgt cacgcagtta tgatttaacg tgatgaggag gaaaaaacgt gtcaagtaat
    10321 gaattattat tagctactga gtatttagaa aaagaaaaga agattcctag agtagtatta
    10381 attgatgcta ttgaagcagc tttaattact gcatacaaaa agaattatga tagtgcaaga
    10441 aatgtccgtg tggaattaaa tatggatcaa ggtactttca aagttatcgc tcgtaaagat
    10501 gttgttgaag aagtatttga cgacagagat gaagtggatt taagtacagc gcttgttaaa
    10561 aaccctgcat atgaaattgg tgatatatac gaagaagatg taacacctaa agattttggt
    10621 cgtgtaggtg ctcaagcagc gaaacaagca gtaatgcaac gtcttcgtga tgctgaacgt
    10681 gaaattttat ttgaagaatt tatagacaaa gaagaagaca tacttactgg aattattgac
    10741 cgtgttgacc atcgttatgt atatgtgaat ttaggtcgta tcgaagctgt tttatctgaa
    10801 gcagaaagaa gtcctaacga aaaatatatt cctaacgaac gtatcaaagt atatgttaac
    10861 aaagtggaac aaacgacaaa aggtcctcaa atctatgttt ctcgtagcca tccaggttta
    10921 ttaaaacgtt tatttgaaca agaagttcca gaaatttacg atggtactgt aattgttaaa
    10981 tcagtagcac gtgaagctgg cgatcgctct aaaattagtg tcttctctga aaacaatgat
    11041 atagatgctg ttggtgcatg tgttggtgct aaaggcgcac gtgttgaagc tgttgttgaa
    11101 gagctaggtg gcgaaaaaat cgacatcgtt caatggaatg aagatccaaa agtatttgta
    11161 aaaaatgctt taagcccttc tcaagtttta gaagttattg ttgatgaaac aaatcaatct
    11221 acagtagttg ttgttcctga ttatcaattg tcattagcga ttggtaaaag aggacaaaac
    11281 gcacgtctag ctgctaaatt aaccggctgg aaaattgata ttaaatcaga aacagatgcg
    11341 cgtgaagtgg gtatctatcc agtagttgaa gctgaaaaag taactgaaga agatattgct
    11401 ttagaagatg ctgacacaac agaatcaacc gaagaggtaa atgatgtttc agttgaaaca
    11461 aatgtagaga aagaatctga ataataggtt ggagtgaagt atctatgaaa aagaaaaaaa
    11521 ttccgatgcg aaaatgtatt ctttcaaatg aaatgcatcc caaaaaagat atgattcgtg
    11581 ttgttgttaa taaagaaggc gaaatcttcg cggatgttac tggaaagaaa caaggccgtg
    11641 gcgcatatgt ttctaaagat gttgctatgg ttgaaaaagc acaacaaaaa gaaattttag
    11701 aaaaatattt taaagcatct aaagagcaat tggatcctgt ttacaaagaa attattagat
    11761 taatttatag agaagagatt ccaaaatgag tatagatcaa atattaaact ttttaggatt
    11821 agcaatgaga gctggtaaag taaaaacagg tgaatcagtc attgttaatg agattaaaaa
    11881 aggaaatttg aagctcgtta ttgttgcaaa tgatgcgtct gataatacag ctaaattaat
    11941 tacagataaa tgtaagagtt acaaagttcc attcagaaag tttggaaatc gaaatgaatt
    12001 gggaatagca cttggaaaag gtgagcgtgt taatgtaggg attactgacc caggctttgc
    12061 taaaaagttg ctatcaatga tagatgaata tcataaggag tgattatatg agtaaacaaa
    12121 gaatttacga atatgcgaaa gaattaaatc taaagagtaa agagattata gatgagttaa
    12181 aaagcatgaa tatagaggtt tcaaatcata tgcaagcttt ggaagatgac caaattaaag
    12241 cattagataa aaagttcaaa aaagaacaaa agaacgacaa taaacaaagc actcaaaata
    12301 atcaccaaaa atcaaacaat caaaaccaaa ataaagggca acaaaaagat aacaaaaaga
    12361 atcaacaaca aaataataaa ggcaacaaag gcaataaaaa gaataataga aataataaga
    12421 aaaataacaa gaataataaa ccacaaaatc aaccagctgc tccaaaagaa ataccatcaa
    12481 aagtgacata tcaagaaggt attacagtag gcgaatttgc ggataaatta aatgttgaat
    12541 catcagaaat tatcaaaaaa ttattcttac ttggtattgt tgctaatatc aatcaatcat
    12601 taaatcaaga aacaatcgaa ttaattgccg atgattatgg cgttgaggtt gaagaagaag
    12661 ttgtgattaa tgaagaagat ttatcaatct acttcgaaga cgaaaaagat gatccagaag
    12721 caattgagag accagcagtt gtaacaatta tgggacatgt tgaccatggt aaaacgactt
    12781 tattagattc aattcgtcat acaaaagtta cagcaggtga agcaggcgga atcactcaac
    12841 atattggtgc atatcaaatt gaaaacgatg gcaaaaaaat cactttctta gatacaccgg
    12901 gacatgctgc atttacaacg atgcgtgcgc gtggtgcaca agtaacagat attacaattt
    12961 tagtagtagc agctgacgat ggtgttatgc cacaaacaat tgaagcaatt aaccatgcta
    13021 aagaagcaga agtaccaatt attgttgcag taaataaaat tgataaacca acttcaaatc
    13081 ctgatcgagt tatgcaagaa ttaactgaat atggtttaat tcctgaagat tggggcggcg
    13141 aaacaatttt cgttccactt tctgcattaa gtggtgatgg tatcgacgat ttattagaaa
    13201 tgataggatt agttgcagaa gttcaagaac ttaaagcaaa tcctaaaaac cgtgctgttg
    13261 gtacagttat cgaagctgaa ttagataaat cacgtggtcc ttctgcatca ttattagtac
    13321 aaaacggtac attaaatgtt ggtgatgcga ttgtagttgg taatacttac ggccgtatcc
    13381 gtgcaatggt taatgactta ggtcaaagaa tcaaaacggc tggtccatca acgcctgttg
    13441 aaattacagg tattaatgat gtgccacaag ctggggatcg ctttgttgta tttagtgatg
    13501 aaaaacaagc tcgtcgtatt ggtgaatcaa gacacgaagc tagcattgta caacaacgtc
    13561 aagaaagtaa aaatgtttca ttagataacc tgtttgaaca aatgaaacaa ggtgaaatga
    13621 aagatttaaa cgttattatt aaaggtgatg ttcaaggttc tgttgaagct ttagctgcat
    13681 cattaatgaa aattgatgtt gaaggcgtaa atgttcgtat cattcataca gcggttggtg
    13741 caattaatga gtcagacgtg acacttgcta atgcctcaaa tggtattatc attggtttca
    13801 atgttcgtcc agacagtggt gcaaaacgtg ctgcagaagc tgaaaatgtt gatatgcgtt
    13861 tacacagagt tatttataat gttatcgaag aaattgaatc agcgatgaaa ggtttacttg
    13921 atccagaatt tgaagaacaa gttatcggac aagctgaagt tcgtcaaaca ttcaaagttt
    13981 ctaaagttgg tactattgct ggatgttatg ttactgaagg taaaattacg cgaaatgctg
    14041 gtgtacgtat tattcgtgat ggtattgttc aatatgaagg cgaattagat acacttaaac
    14101 gtttcaaaga tgatgctaag gaagttgcaa aaggttatga atgtggtatt acaattgaaa
    14161 actacaatga ccttaaagaa ggcgatgtta tcgaagcatt tgaaatggtt gaaattaagc
    14221 gttaattaaa taaattacaa gctaaaagta tagttaagat tgatatgctc cctataaata
    14281 ttgcactttt taagtgtcta ctttataggg agcatatttg atactagctt ttggtttttt
    14341 attagaatag attacctatt aaaagttacg ttatatggac atgattttgt ataaaatttt
    14401 gtggtggcct agaatgattt ttaatgacaa aatataatgt cgactattat tggaaaattt
    14461 tctgttgaaa tgcctatctt acggcaaact ttatttgatt ttataggctt aatttattaa
    14521 aataacgtgt gagctaaaat aattgtttaa gcattgttac actaaaaaat gcaaataaca
    14581 attgaactta aagataaaga ggtgacaaga atgagcagta tgagagcaga gcgtgttggt
    14641 gaacaaatga agaaggaatt aatggatatc atcaacaata aagtcaaaga tcctcgagtt
    14701 ggttttatta caattacaga tgttgtttta acaaatgatt tatcgcaggc taaagtattt
    14761 ttaactgtat taggtaacga taaagaagta gaaaatacat ttaaagcact tgataaagca
    14821 aaaggcttca ttaagtctga attaggttct agaatgcgat tacgtattat gccggaatta
    14881 atgtatgaat atgatcaatc aatcgaatat ggtaataaaa ttgaacgaat gattcaagat
    14941 ttacacaaac aagatagata atttagtgtt aggtatctgg aaaatgtttg ataatttctt
    15001 aatatcggta tattaacatt aaacagttaa tacatagatg tgtagaaata gttaacattt
    15061 tccagttttt ttatgaataa attcagttga tacgctatta aaatatattt taaaaaagaa
    15121 ggtgactata tgtataatgg gatattacca gtatataaag agcgcggttt aacaagtcat
    15181 gacgttgtat tcaaattgcg taaaatatta aaaactaaaa aaataggtca cacgggtacg
    15241 cttgatcccg aagttgcagg cgtgttaccg gtatgtatag gtaatgcaac gagagttagt
    15301 gattatgtta tggatatggg caaagcttat gaagcaactg tatcgatagg aagaagtaca
    15361 acgactgaag atcaaacggg tgatacattg gaaacaaaag gtgtacactc agcagatttt
    15421 aataaggacg atattgaccg attgttagaa aattttaaag gtgttattga acaaattccg
    15481 ccaatgtatt catcagtcaa ggtgaatggc aaaaaattat atgaatatgc gcgtaataat
    15541 gaaacagttg aaagaccaaa gcgtaaagtt aatattaaag acattgggcg tatatctgaa
    15601 ttagatttta aagaaaatga gtgtcatttt aaaatacgcg tcatctgtgg taaaggtaca
    15661 tatattagaa cgctagcaac tgatattggt gtgaaattag gctttccggc acatatgtcg
    15721 aaattaacac gaatcgagtc tggtggattt gtgttgaaag atagccttac attagaacaa
    15781 ataaaagaac ttcatgagca ggattcattg caaaataaat tgtttccttt agaatatgga
    15841 ttaaagggtt tgccaagcat taaaattaaa gattcgcaca taaaaaaacg tattttaaat
    15901 gggcagaaat ttaataaaaa tgaatttgat aacaaaatta aagaccaaat tgtatttatt
    15961 gatgatgatt cagaaaaagt attagcaatt tatatggtac accctacaaa agaatcagaa
    16021 attaaaccta aaaaagtctt taattaaagg agatagaatt tatgaaagtc atagaagtga
    16081 cacatcctat acaatctaaa cagtatatta cagaggatgt tgcaatggca ttcggatttt
    16141 tcgatggcat gcataaaggt catgacaaag tctttgatat attaaatgaa atagctgagg
    16201 cacgcagttt aaaaaaaacg gtgatgacat ttgatccgca tccgtctgtc gtgttgaatc
    16261 ctaaaagaaa acgaacaacg tatttaacac cactttcaga taaaatcgaa aaaattagcc
    16321 aacatgatat tgattattgt atagtggtta atttttcatc taggtttgct aatgtgagcg
    16381 ttgaagattt tgttgaaaat tatataatta aaaataatgt aaaagaagtc attgctggtt
    16441 ttgattttac ttttggtaaa tttggaaaag gtaatatgac tgttcttcaa gaatatgatg
    16501 cgtttaatac gacaattgtg agtaaacaag aaattgaaaa tgaaaaaatt tctacaactt
    16561 ctattcgtca agatttaatc aatggtgagt tgcaaaaagc gaatgatgct ttaggctata
    16621 tttattctat taaaggcact gtagtgcaag gtgaaaaaag gggaagaact attggcttcc
    16681 caacagctaa cattcaacct agtgatgatt atttgttacc tcgtaaaggt gtttatgctg
    16741 ttagtattga aatcggcact gaaaataaat tatatcgagg ggtagctaac ataggtgtaa
    16801 agccaacatt tcatgatcct aacaaagcag aagttgtcat cgaagtgaat atctttgact
    16861 ttgaggataa tatttatggt gaacgagtga ctgtgaattg gcatcatttc ttacgtcctg
    16921 agattaaatt tgatggtatc gacccattag ttaaacaaat gaacgatgat aaatcgcgtg
    16981 ctaaatattt attagcagtt gattttggtg atgaagtagc ttataatatc tagagttgcg
    17041 tatagttata taaacaatct ataccacacc tttttcttag taggtcgaat ctccaacgcc
    17101 taactcggat taaggagtat tcaaacattt taaggaggaa attgattatg gcaatttcac
    17161 aagaacgtaa aaacgaaatc attaaagaat accgtgtaca cgaaactgat actggttcac
    17221 cagaagtaca aatcgctgta cttactgcag aaatcaacgc agtaaacgaa cacttacgta
    17281 cacacaaaaa agaccaccat tcacgtcgtg gattattaaa aatggtaggt cgtcgtagac
    17341 atttattaaa ctacttacgt agtaaagata ttcaacgtta ccgtgaatta attaaatcac
    17401 ttggtatccg tcgttaatct taatataacg tctttgaggt tggggcatat ttatgttcca
    17461 accttaattt atattaaaaa agctttttac aaatattaac atttattata tgttaagcta
    17521 atatt
//
LOCUS       DAHHSC010000038        17132 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.038, whole genome shotgun sequence.
ACCESSION   DAHHSC010000038 DAHHSC010000000
VERSION     DAHHSC010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 17132)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17132)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17132
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.038"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            complement(142..1020)
                     /locus_tag="NBI57_000962"
     CDS             complement(142..1020)
                     /locus_tag="NBI57_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002445753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE2"
                     /protein_id="HCD1821926.1"
                     /translation="MTEFDLSTREGRWKHFGSVDPIEGTKPTTKNEMTDLQSTHKDFL
                     FEIEEVGIKNLVYPVLVDQYQTAGTFSFSTSLTKDEKGINMSRIIESVEKHYDNGIEL
                     EFNTLYQVLRTLQTNMKQNAAGVDVSGKWFFDRYSPTTNIKAVGNADVTYGLAIDGDK
                     VTRKELTIEATVTTLCPCSKEISEYSAHNQRGVVTVKTYINKDQNIVDDYKNKILDAM
                     EANASSILYPILKRPDEKRVTERAYENPRFVEDLIRLIAADLVEFDWLDGFDIECRNE
                     ESIHQHDAFAKLKYRK"
     gene            complement(1033..1698)
                     /gene="bshB2"
                     /locus_tag="NBI57_000963"
     CDS             complement(1033..1698)
                     /gene="bshB2"
                     /locus_tag="NBI57_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacillithiol biosynthesis deacetylase BshB2"
                     /protein_id="HCD1821927.1"
                     /translation="MTDERHVLVIFPHPDDETFSSAGTLASYIQKGIPVTYACLTLGQ
                     MGRNLGNPPFATRESLPSIRERELEEACKVIGITDLRKMGLRDKTVEFEPYEHIDGMI
                     KSLIDDTNPSLIISFYPGYAVHPDHEATADAVIRTVERMPKEERPRLTLVAFSNDASE
                     ALGEPDIQNDITDFKELKIKAFEAHASQTGPFLKQLASPEIDGKQHSFLTVEPYWTYY
                     FKS"
     gene            complement(1712..2074)
                     /locus_tag="NBI57_000964"
     CDS             complement(1712..2074)
                     /locus_tag="NBI57_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YojF family protein"
                     /protein_id="HCD1821928.1"
                     /translation="MLEPIKEQEVIDLLASFEHKPVYLHVETTNGAYANHFDQRVFNA
                     GTFLRNIQVTYTHAQLKGGNKEPYRIGLKLSNGGWVYVQGLTHFEVNEHDEFLIAGFN
                     YEGQLAAALQISERPFNL"
     gene            2350..3108
                     /gene="nagB"
                     /locus_tag="NBI57_000965"
     CDS             2350..3108
                     /gene="nagB"
                     /locus_tag="NBI57_000965"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HCD1821929.1"
                     /translation="MKVLNLGSKKQASFYVACELYKEMAFNQHCKLGLATGGTMTDLY
                     EQLVKLLNKNQLNVDNVSTFNLDEYVGLTASHPQSYHYYMDDMLFKQYPYFNRKNIHI
                     PNGDADDMNAEASKYNDVLEQQGQRDIQILGIGENGHIGFNEPGTPFDSVTHIVDLTE
                     STIKANSRYFKNEDDVPKQAISMGLANILQAKRIILLAFGEKKRAAITHLLNQEISVD
                     VPATLLHKHPNVEIYLDDEACPKNVAKIHVDEMD"
     gene            3185..3817
                     /gene="hxlA"
                     /locus_tag="NBI57_000966"
     CDS             3185..3817
                     /gene="hxlA"
                     /locus_tag="NBI57_000966"
                     /EC_number="4.1.2.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019834869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hexulose-6-phosphate synthase"
                     /protein_id="HCD1821930.1"
                     /translation="MELQLAIDLLNKEDAAELANKVKDYVDIVEIGTPIIYNEGLPAV
                     KHMADNISNVKVLADMKIMDAADYEVSQAIKFGADVITILGVAEDASIKAAIEEAHKN
                     NKQLLVDMIAVQDLEKRAKELDEMGADYIAVHTGYDLQAEGQSPLESLRTVKSVIKNS
                     KVAVAGGIKPDTIKDIVAESPDLVIVGGGIANADDPVEAAKQCRAAIEGK"
     gene            3819..4367
                     /gene="hxlB"
                     /locus_tag="NBI57_000967"
     CDS             3819..4367
                     /gene="hxlB"
                     /locus_tag="NBI57_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-3-hexuloisomerase"
                     /protein_id="HCD1821931.1"
                     /translation="MAKFSDYQLILDELKMTLSHVEADEFSTFASKILHAEHIFVAGK
                     GRSGFVANSFAMRLNQLGKQAHVVGESTTPAIKSNDVFVIISGSGSTEHLRLLADKAK
                     SVGADIVLITTNKDSAIGNLAGTNIVLPAGTKYDEQGSAQPLGSLFEQASQLFLDSVV
                     MGLMTEMNVTEQTMQQNHANLE"
     gene            4480..5127
                     /locus_tag="NBI57_000968"
     CDS             4480..5127
                     /locus_tag="NBI57_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HCD1821932.1"
                     /translation="MKFDNYIFDFDGTLADTKKCGEVATQSAFKACGLTEPSSKEITH
                     YMGIPIEESFLKLADRPLDEAALAKLIDTFRHTYQSIEKDYIYEFAGITEAITSLYNQ
                     GKKLFVVSSKKSDVLERNLSAIGLNHLITEAVGSDQVSAYKPNPEGIHTIVQRYNLNS
                     QQTVYIGDSTFDVEMAQHAGVPSAAVTWGAHDARSLLHSNPDFIINDPSEINTVL"
     gene            5629..7029
                     /locus_tag="NBI57_000969"
     CDS             5629..7029
                     /locus_tag="NBI57_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCD1821933.1"
                     /translation="MDFNKENINMVDAKKAKKTVVATGIGNAMEWFDFGVYAYTTAYI
                     GANFFSPVENADIRQMLTFAALAIAFLLRPIGGVVFGIIGDKYGRKVVLTSTIILMAF
                     STLTIGLLPSYDQIGLWAPILLLLARVLQGFSTGGEYAGAMTYVAESSPDKRRNSLGS
                     GLEIGTLSGYIAASIMIAVLTFFLTDEQMASFGWRIPFLLGLFLGLFGLYLRRKLEES
                     PVFENDVATQPERDNINFLQIIRFYYKDIFVCFVAVVFFNVTNYMVTAYLPTYLEQVI
                     KLDATTTSVLITCVMAIMIPLALMFGKLADKIGEKKVFLIGTGGLTLFSIIAFMLLHS
                     QSFVVIVIGIFILGFFLSTYEATMPGSLPTMFYSHIRYRTLSVTFNISVSIFGGTTPL
                     VATWLVTKTGDPLAPAYYLTAISVIGFLVITFLHLSTAGKSLKGSYPNVDNEQDRAYY
                     AEHPKEALWWVKERKN"
     gene            7573..8949
                     /locus_tag="NBI57_000970"
     CDS             7573..8949
                     /locus_tag="NBI57_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HCD1821934.1"
                     /translation="MNVILEQLKTHTQNKPNDIALHIDDETITYSQLNARITSAVESL
                     QKYSLNPVVAINMKSPVQSIICYLALHRLHKVPMMMEGKWQSTIHRQLIEKYGIKDVI
                     GDTGLMQNIDSPMFIDSTQLQHYPNLLHIGFTSGTTGLPKAYYRDEDSWLASFEVNEM
                     LMLKNENAIAAPGPLSHSLTLYALLFALSSGRTFIGQTTFHPEKLLNQCHKISSYKVA
                     MFLVPTMIKSLLLVYNNEHTIQSFFSSGDKLHSSIFKKIKNQANDINLIEFFGTSETS
                     FISYNLNQQAPVESVGVLFPNVELKTTNHDHNGIGTICIKSNMMFSGYVSEQCINNDE
                     WFVTNDNGYVKEQYLYLTGRQQDMLIIGGQNIYPAHVERLLTQSSSIDEAIIIGIPNE
                     RFGQIGVLLYSGDVTLTHKNVKQFLKKKVKRYEIPSMIHHVEKMYYTASGKIAREKMM
                     SMYLRGEL"
     gene            8951..10090
                     /locus_tag="NBI57_000971"
     CDS             8951..10090
                     /locus_tag="NBI57_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HCD1821935.1"
                     /translation="MNQAVIVAAKRTAFGKYGGTLKHLEPEQLLKPLFQHFKEKYPEV
                     ISKIDDVVLGNVVGNGGNIARKALLEAGLKDSIPGVTIDRQCGSGLESVQYACRMIQA
                     GAGKVYIAGGVESTSRAPWKIKRPHSVYETALPEFYERASFAPEMSDPSMIQGAENVA
                     KMYDVSRELQDEFAYRSHQLTAENVKNGNISQEILPITVKGEIFNTDESLKSHIPKDN
                     FGRFKPVIKGGTVTAANSCMKNDGAVLLLIMEKDMAYELGFEHGLLFKDGVTVGVDSN
                     FPGIGPVPAISNLLKRNQLTIENIEVIEINEAFSAQVVACQQALNISNTQLNIWGGAL
                     ASGHPYGASGAQLVTRLFYMFDKETMIASMGIGGGLGNAALFTRF"
     gene            10065..10430
                     /locus_tag="NBI57_000972"
     CDS             10065..10430
                     /locus_tag="NBI57_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein VraC"
                     /protein_id="HCD1821936.1"
                     /translation="MQHYLLDSNQRLNVSFSKDSVAAYYQCFNQPYRKEVPPLMCASL
                     WPKFDLFKKYANSELILTKSAINQTQKIEVDTIYVGHLEDIECRQTRNITRYTMALTL
                     TKNDQHVITVTQTFIKAMK"
     gene            10433..10702
                     /locus_tag="NBI57_000973"
     CDS             10433..10702
                     /locus_tag="NBI57_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1821937.1"
                     /translation="MKFNEIWINEYLALVNDDNPIHNEIVPGQLVSQMMLMAMSLETN
                     QCQINYVKPILINENIEFIEQHEHEIIAINDDGEIKIKISLSTKK"
     gene            complement(10903..11070)
                     /locus_tag="NBI57_000974"
     CDS             complement(10903..11070)
                     /locus_tag="NBI57_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein VraX"
                     /protein_id="HCD1821938.1"
                     /translation="MIIYRQYHHEGAPVYEIITKTFQHVSIKCDDSFSDTEIFKLLSL
                     LQDDIDHMKVS"
     gene            complement(11577..12407)
                     /gene="thiD"
                     /locus_tag="NBI57_000975"
     CDS             complement(11577..12407)
                     /gene="thiD"
                     /locus_tag="NBI57_000975"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001185456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HCD1821939.1"
                     /translation="MALKKVLTIAGSDTSAGAGMQADLKTFQELDTYGMVALTAIVTM
                     DKDTWSHDVTPLPMDVFEKQLETALSIGPDAIKTGMLGTEEIIKRAGEVYEASNAQYF
                     VVDPVMVCKGEDEVLNPGNTEAMIKYLLPKATVVTPNLFEAGQLSGLGKLNSIEDMKK
                     AATIIFDKGAQHVIIKGGKALDQDKSYDLYYDGQTFYQLTTDMFQQSYNHGAGCTFAA
                     ATTAYLANGKSPKEAVISAKAFVASAIKNGWKMNDFVGPVDHGAYNRIEHIDVEVTEV
                     "
     gene            12591..13247
                     /locus_tag="NBI57_000976"
     CDS             12591..13247
                     /locus_tag="NBI57_000976"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HCD1821940.1"
                     /translation="MEWSQIFHDITTKHDFKAMHDFLEKEYSTAIVYPDRENIYQAFD
                     LTPFENIKVVILGQDPYHGPNQAHGLAFSVQPNAKFPPSLRNMYKELADDIGCVRQTP
                     HLQDWAREGVLLLNTVLTVRQGEANSHRDIGWETFTDEIIKAVSDYKEHVVFILWGKP
                     AQQKIKLIDTSKHCIIKSVHPSPLSAYRGFFGSKPYSKANAYLESVGKSPINWCESEA
                     "
     gene            13248..13628
                     /locus_tag="NBI57_000977"
     CDS             13248..13628
                     /locus_tag="NBI57_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5327 family protein"
                     /protein_id="HCD1821941.1"
                     /translation="MVNRETLIARIEQELVQAEQAQHDHDFEKHMYAIHILTSLYAST
                     SNTPHIGEQQMNRRIANHNQMPQSQITQPTHQVTAAEIEAMGGKVNTHSAHHHNKSYS
                     QPSNQQQRLATDDDIGNGESIFDF"
     gene            13761..14129
                     /locus_tag="NBI57_000978"
     CDS             13761..14129
                     /locus_tag="NBI57_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HCD1821942.1"
                     /translation="MKLFIILGALNAMMAVGTGAFGAHGLQGKISDHYLSVWEKATTY
                     QMYHGLALLIIGVISGTTSINVNWAGWLIFAGIIFFSGSLYILVLTQIKVLGAITPIG
                     GVLFIIGWIMLIIATFKFAG"
     gene            14250..15734
                     /locus_tag="NBI57_000979"
     CDS             14250..15734
                     /locus_tag="NBI57_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HCD1821943.1"
                     /translation="MEKKNKQIDRGDLKQNLSEKFVWAIAYGSCIGWGAFILPGDWIK
                     QSGPIAASIGIVIGALLMILIAVSYGALVERFPVSGGAFAFSFLSFGRYVSFFSSWFL
                     TFGYVCVVALNATAFSLLVKFLLPDVLNNGKLYTIAGWDVYITEIIIATVLLLVFMLV
                     TIRGASVSGSLQYYFCVAMVIVVLLMFFGSFFGNNFALENLQPLAEPSKGWLVSIVVI
                     VSVAPWAYVGFDNIPQTAEEFNFAPNKTFKLIVYSLLAASLTYVVMILYTGWLSTSHQ
                     SLNGQLWLTGAVTQTAFGYIGLGVLAIAIMMGIFTGLNGFLMSSSRLLFSMGRSGIMP
                     TMFSKLHSKYKTPYVAIIFLVGVSLIAPWLGRTALTWIVDMSSTGVSIAYFITCLSAA
                     KLFSYNKQSNTYAPVYKTFAIIGSFVSFIFLALLLVPGSPAALTAPSYIALLGWLIIG
                     LIFFVIRYPKLKNMDNDELSRLILNRSENEVDDMIEEPEKEKTK"
     gene            complement(15875..16327)
                     /locus_tag="NBI57_000980"
     CDS             complement(15875..16327)
                     /locus_tag="NBI57_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HCD1821944.1"
                     /translation="MFWILNFIFSFLASMFFCVIFDAPRKLYLSCGFVGTCGWMVYTL
                     FFNGFNVHTIYSSFFGSLALGLLSHYMARKQKEPAIIFMVTGIIPLVPGGLAYDATKN
                     LVLLNFSTAINTMLEVTLIAGAIALGLLFADQISKLIVSGFVKSFKRL"
     gene            complement(16340..17104)
                     /locus_tag="NBI57_000981"
     CDS             complement(16340..17104)
                     /locus_tag="NBI57_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter ThrE family protein"
                     /protein_id="HCD1821945.1"
                     /translation="MDINSEEYKQEVLIKDVVMLAARILLESGAEGTRVEDTMTRIAK
                     KLGYSESNSFVTNTVIQFTLHSESFPRIFRITSRDTNLIKISQANKISRQITNNEISL
                     AEAKTQLEKIYVAKRDSSLPFKGFAAAMIAMSFLYLQGGRLIDVLTAILAGSLGYLVT
                     EILDRKLHAQFIPEFIGSLVIGIIAVIGHTLIPTGDLATIIIAAVMPIVPGVLITNAI
                     QDLFGGHMLMFTTKSLEALVTAFGIGAGVGSVLILV"
BASE COUNT         5800 a         2640 c         3119 g         5573 t
ORIGIN      
        1 aatatatcat tgtagagctt aggatattga tttatgcctc aggcacatca aatgagaaga
       61 tttataaaag agatatacaa ctctagaagg tataataaaa acgcgcaact aatgttacgc
      121 gtttgaatta atcatatgat attatttgcg atactttaat ttagcgaaag catcatgttg
      181 atggatagac tcttcattac gacattcgat atcgaaaccg tctaaccaat caaattcaac
      241 taagtccgcg gcaattaaac gaattaagtc ttcgacaaaa cgtggatttt catatgcacg
      301 ctctgtcaca cgtttttcat caggacgttt taaaataggg tatagaattg aacttgcatt
      361 agcttccatt gcatctaaaa ttttgttttt atagtcatca actatgtttt gatctttatt
      421 aatatatgtt ttaacagtga caacaccacg ttggttgtgc gctgaatact cacttatttc
      481 ttttgaacaa gggcatagcg ttgtgacagt tgcttcaata gtaagttctt tacgtgtaac
      541 tttatcaccg tcaattgcta atccataagt gacatcggca ttaccaactg ctttaatatt
      601 tgtggttgga ctatagcggt caaagaacca tttcccagaa acatcaacgc ctgccgcatt
      661 ttgtttcata ttcgtttgta aagtgcgtaa cacctgataa agtgtattaa attcaagttc
      721 aataccatta tcatagtgct tttcaacact ttcaattata cggctcatat taataccttt
      781 ttcgtctttt gttaaacttg ttgaaaaact aaatgtgcca gctgtttgat actggtcaac
      841 aagtacaggg tacactaagt ttttaatacc aacttcttct atttcaaata aaaaatcttt
      901 atgtgtactt tgtaaatctg tcatttcgtt cttagtagta ggtttcgtgc cttcaatagg
      961 atctacggaa ccaaagtgtt tccaacgacc ttctcgtgtc gataaatcaa attcagtcat
     1021 ttttttcctc cgttaagatt taaagtaata tgtccaatat ggttcgactg ttaaaaagct
     1081 gtgttgttta ccatcgattt caggacttgc taattgtttt aaaaatggac ctgtttgaga
     1141 agcatgtgct tcaaatgcct taattttaag ttctttaaaa tctgtaatat cattttgaat
     1201 atcaggttct ccaagagctt cggatgcatc attactgaac gcaactaaag ttaaacgagg
     1261 gcgttcttct ttaggcatgc gttcaaccgt tcgaattaca gcgtctgctg ttgcttcgtg
     1321 atcaggatgt actgcatatc caggataaaa tgaaataatc aatgatggat ttgtatcatc
     1381 gattaaagat ttaatcatac catctatatg ttcgtagggt tcaaattcga cagttttgtc
     1441 acgtaaaccc atttttctta aatcagtaat accaataact ttacaagctt cttctagttc
     1501 acgctcacga atacttggta atgattcgcg tgttgcaaat gggggattac ctaaatttct
     1561 gcccatttgt cctagggtta aacatgcata tgttacaggt atgccttttt ggatataact
     1621 tgctaatgtg cctgcagatg agaaggtttc atcatcagga tgtggaaata ttactaatac
     1681 atgtctttcg tcagtcatgt tgatgcctcc tctataaatt aaatggtcgc tcactaattt
     1741 gaagtgctgc agcgagttga ccttcgtaat taaaacctgc aattaaaaat tcatcatgtt
     1801 cattgacctc aaaatgcgtt agaccttgta cataaaccca accaccattt gatagtttaa
     1861 gaccaatgcg ataaggttct ttattaccac cttttagttg tgcatgcgta tatgttactt
     1921 gtatgtttct taaaaatgta ccagcattaa aaacacgttg atcgaaatgg ttcgcatagg
     1981 ccccatttgt cgtttcaaca tgcagataca caggtttatg ttcaaaagaa gcaagtaaat
     2041 ctataacttc ttgttcttta attggttcca acacgttcac tccttacact atcaatgtgt
     2101 ttatctttct attttactaa aaactattcg ataattgtat acgattgctc aattatttat
     2161 aaattaattt tcatgaaggg taattactca ggattacgta atcatacagc attagttttt
     2221 tacttttaaa aatcaaaaat ttgttggaat ttgaaaagtg ttaaacatta aaaatgatgc
     2281 tatattaatg gtgtatgaat gaattcataa gtttttaaaa tgtattaaat ttgtggaggc
     2341 atgtaaacaa tgaaagtatt aaacttagga tcgaaaaaac aagcatcatt ctatgttgca
     2401 tgtgagttat ataaagagat ggcatttaat cagcactgta aactaggttt agcaactggt
     2461 ggtacaatga cagatttgta tgaacaactt gttaagttat taaataaaaa tcagttaaac
     2521 gtagacaatg tatccacgtt taatttagac gaatatgtag gtttaaccgc atcacatccg
     2581 caaagttatc actattatat ggatgacatg cttttcaaac aatatcctta ttttaataga
     2641 aagaacattc atattccaaa tggagatgcc gatgatatga atgcggaagc gtcaaaatat
     2701 aatgacgttt tagaacaaca aggtcaacgt gatattcaaa ttttaggtat tggtgaaaat
     2761 ggtcatattg gatttaatga acctggtacg ccgtttgata gcgttactca tatcgttgat
     2821 ttgactgaaa gtactattaa ggctaatagt cgatatttta aaaacgaaga tgatgttcca
     2881 aagcaagcca tttcgatggg acttgctaat attcttcaag ccaaacgtat cattttactc
     2941 gcatttggtg aaaagaaacg tgctgctatt acacatttat taaatcagga aatttctgtt
     3001 gatgttccag ccacattact tcacaaacac ccgaatgttg agatatattt agacgacgaa
     3061 gcttgcccga aaaatgttgc gaaaattcat gtcgatgaaa tggattgatt gcaatgttta
     3121 attaagaaat gcctcgggaa aggttccaat agaaagataa aaagcattgg aaggatgatt
     3181 tttagtggaa ttacaattag caattgattt attaaacaaa gaagacgcgg ctgagttagc
     3241 aaataaagta aaagattatg tagatatcgt agaaatcggt acgccaatca tttacaacga
     3301 aggtttacca gcagttaaac atatggcaga caacattagt aatgtaaaag tattagcaga
     3361 catgaaaatt atggatgcag ctgattatga agttagccaa gcaattaaat ttggcgcgga
     3421 tgtaattaca atactaggtg ttgcagaaga tgcatcaatt aaagcagcta ttgaagaagc
     3481 tcataaaaat aataaacaat tactagttga tatgattgct gttcaagatt tagaaaaacg
     3541 tgcaaaagaa ctagatgaaa tgggtgctga ttatattgca gtacacactg gttatgattt
     3601 acaagcagaa gggcaatcac cattagaaag tttaagaacc gttaaatctg ttattaaaaa
     3661 ttctaaagtt gcagtagcag gtggaattaa accagataca attaaagata ttgtcgctga
     3721 aagtcctgat cttgttattg ttggtggcgg aatcgcaaat gcagatgatc cagtagaagc
     3781 tgcaaaacaa tgtcgcgctg caatcgaagg taagtaatat ggctaaattt agtgactatc
     3841 aattaattct agatgaatta aagatgactt tgtcacatgt tgaagcggat gagttttcaa
     3901 cttttgcatc caaaatacta catgctgaac atatatttgt agctggcaaa ggacgttcag
     3961 gattcgtggc gaatagtttt gcaatgcgct taaatcagct cggcaaacag gcacatgttg
     4021 ttggagaatc aacgacacct gcgattaagt cgaatgatgt atttgtaatt atctctggtt
     4081 caggttccac ggaacattta agattattag cagacaaagc aaaatcagta ggtgctgaca
     4141 tcgtattaat tactacaaat aaagattctg caataggcaa tctagctggg acgaacatcg
     4201 ttttgcctgc aggtacaaaa tatgatgaac aaggctcggc acaaccatta ggaagtttgt
     4261 ttgaacaagc atctcaatta tttttagata gtgttgtaat gggattgatg actgaaatga
     4321 atgttacgga acaaacgatg caacaaaatc atgctaattt agaataaaat aaagatagtc
     4381 gataatatga tgcctaggca gaaatattat cgattatttt tttatttaaa taataaatta
     4441 tagtataata tcaataataa acgaataggg gtgttaatat tgaagtttga caattacatt
     4501 tttgattttg atggtacgtt ggcagacacg aaaaaatgtg gtgaagtagc aacacaaagt
     4561 gcatttaaag catgtggctt aacggaacca tcatctaaag aaataacgca ttatatggga
     4621 atacctattg aagaatcatt tttaaaatta gcagaccgac cattagatga agcagcatta
     4681 gcaaagttaa tcgatacatt tagacataca tatcaatcta ttgaaaagga ctatatttat
     4741 gaatttgcgg gtataactga agccattaca agtttgtata accaagggaa aaaacttttc
     4801 gtggtgtcta gtaagaagag tgatgtatta gaaagaaatt tatcggctat tggattaaat
     4861 cacttgatta ccgaagctgt tggatccgat caagtaagtg catataaacc aaatcctgaa
     4921 ggcatacaca caattgtgca acgctacaat ttaaatagcc aacaaacggt ttatattggt
     4981 gattcaacat ttgatgttga gatggcgcaa catgctggtg taccatctgc agcagtaaca
     5041 tggggagcac atgatgcaag gtcattactt cattcaaatc cagattttat aatcaatgat
     5101 ccatcagaaa ttaatacagt attataaaat ttgttaaaac agagaatacc atggttaaaa
     5161 tgcatattca taaatattag attatacata gaaatatttc gctttagatt aggaatgtaa
     5221 aataaatatt tattaataat cgtgtatgtt taaagagtga tgtctgattc gttgatcatt
     5281 tatttttgta caaataaatt aaagtaaaaa caaagttatt gaagtaaatc gaatataaac
     5341 attttaatat gatgaggaaa gtagtgatag catatgactt acatgaagtt gtgtgctatc
     5401 gctattttta gttataattc caaaaagtta atcgttcgat gatttaagaa ttattattgt
     5461 ttaattcaaa tgtatgaggg tataaaatca ttgaatttaa ttcgataaag tgaaattttt
     5521 gaacaaacat acttttgtat ttatataaaa gtttaaattc ttataaattt gacaaaacta
     5581 attaactccg tataattatg aaacatacaa gagggagtgt atgaattcat ggattttaat
     5641 aaagagaata ttaacatggt ggatgcaaag aaagctaaaa aaaccgttgt tgcaaccggt
     5701 atcggtaatg caatggaatg gttcgatttt ggtgtctatg catatacaac tgcgtacatt
     5761 ggagcgaact tcttctctcc agtagagaat gcagacattc gacaaatgtt gactttcgca
     5821 gcattagcca ttgcgttttt attaagacca attggtggtg tggtatttgg tattattggt
     5881 gacaaatatg gacgtaaagt tgtattaaca tctacaatta ttttaatggc attttcaaca
     5941 ttaaccattg gattattgcc aagctatgat caaattggac tttgggcacc aatactatta
     6001 ttgcttgcaa gagtactaca agggttttca acaggtggag agtatgcggg ggcaatgaca
     6061 tatgttgcgg agtcatctcc agataagcgt cgtaactcat taggtagtgg actagaaatt
     6121 gggacattat caggttacat agctgcttca attatgattg cagtattaac attcttttta
     6181 acagatgaac aaatggcatc atttggttgg agaatcccat tcttacttgg tttattccta
     6241 ggattattcg gcttatattt acgtcgtaag cttgaagaat caccagtttt cgaaaatgat
     6301 gttgcaacac aaccagaacg agacaacatt aactttttac aaatcatcag attttattac
     6361 aaagatatat ttgtgtgttt tgtagctgtt gtattcttca atgttacgaa ctatatggta
     6421 actgcatatt taccaaccta tttagaacaa gttattaaat tagatgcaac gacaacaagt
     6481 gtattaatta cttgtgtcat ggcaataatg attccattag cattaatgtt tggtaagtta
     6541 gcggataaaa taggtgaaaa gaaagtattt ctaattggta ctggtgggct aacattattt
     6601 agtatcatcg catttatgtt attacattca caatcatttg ttgtaatagt aatcggtata
     6661 tttatattag gatttttctt atcaacttac gaagcgacaa tgccagggtc gttaccaacg
     6721 atgttttaca gtcatataag atatcgaact ttatcagtaa catttaatat ctctgtttcg
     6781 atatttggtg gtacgacgcc attagttgca acatggttag ttacgaaaac tggagatcca
     6841 ttagcacctg cgtattattt aacagcaatc agtgttattg gctttttagt tattacattc
     6901 ttacatttaa gtacagcagg aaaatctcta aaaggttcgt atccaaatgt agataacgag
     6961 caagatagag cttattatgc agaacaccca aaagaagcat tatggtgggt taaagaacgt
     7021 aagaattaga gattttaata aaaagtataa atcaatcgta tataagcact ttaaagctag
     7081 taggttctgc taactttaaa gtgcttttta aattgagaac tgtaattagc cgtaataaag
     7141 tttttgtata tacataaacc cccactgcaa tgattatcgc aatgggggaa agaggggact
     7201 taaagcatat gtttagcttt gaatacttaa aattctcttg ctattgaaat gttaggatgt
     7261 aaatatgtct tagagtattt tgtccaacgc aattaatatt gagactctaa ccttcaatat
     7321 tattatagag aacacaaact taaatagatt gggtgactta tttgtgtcag ttattgcgat
     7381 tgcgataact tcttttctct atatacatat agtaacgtct tatctaataa aaaacatggt
     7441 actacagtat caaatttatc tagggcttaa gtttgatttt tataataggc aggtttacct
     7501 gataaaaata cttattcatt atataatgtt aacaagatgt attttaaagt ttacattgag
     7561 tgagggatat tgatgaacgt aattttagaa cagttgaaaa cacatactca aaataaacct
     7621 aatgacatag cattacatat cgatgatgaa acaattacat atagtcaact aaatgcccgc
     7681 atcactagcg cagttgaatc tttgcagaaa tattcactta accctgtcgt tgctattaat
     7741 atgaaatcac cggtgcaaag tattatttgt tatttagctt tgcatcgttt acataaagtg
     7801 cctatgatga tggaaggtaa atggcaaagt actatacatc gtcaattgat tgaaaaatat
     7861 ggtattaaag atgtaattgg agatacaggt ctcatgcaga atatagactc accgatgttt
     7921 attgattcaa cgcaattaca gcactacccc aatttattac atattggttt tacttcaggg
     7981 acaactggac tgccaaaagc atattatcgt gatgaagatt catggttggc ttcttttgaa
     8041 gttaatgaaa tgttgatgtt aaaaaatgaa aatgcaatag cagcccctgg accactatcg
     8101 cactcgttaa cattatatgc gttattgttt gctttaagtt ccggtcgtac ttttatagga
     8161 cagaccactt ttcatcctga aaagttactt aatcaatgtc ataaaatatc atcatacaaa
     8221 gttgctatgt ttcttgttcc aacgatgatt aaatcattat tgttagttta caacaatgaa
     8281 catacaatcc aatcattttt tagcagtgga gataagctgc attcttctat ttttaaaaag
     8341 ataaaaaatc aagcaaatga cataaatttg attgaatttt ttggtacatc ggaaaccagt
     8401 tttatcagct ataacttgaa tcagcaagca ccagttgaat cagtaggtgt gctatttcca
     8461 aatgtggaat tgaaaacaac gaatcacgat cacaatggta taggaactat ttgtataaaa
     8521 agtaatatga tgtttagtgg ctatgtaagt gaacaatgta taaataatga tgaatggttt
     8581 gttactaatg ataatggcta tgtaaaagag cagtatttat atttaacggg acgtcaacag
     8641 gatatgttaa ttattggtgg tcaaaatata tatccagcac atgttgaacg ccttttaacg
     8701 caatcttcga gcattgatga agcaattatc atcggtattc caaatgagcg ttttggtcaa
     8761 ataggcgtat tgctttattc tggtgatgtg acacttacac ataaaaatgt aaaacaattt
     8821 ttaaaaaaga aagtgaaacg ctatgaaatt ccatcgatga ttcatcatgt agaaaagatg
     8881 tattacactg caagtggtaa aattgctaga gaaaaaatga tgtcgatgta tttgagaggt
     8941 gaattataat atgaatcaag cagtcatagt tgcagctaaa cgaactgcat ttgggaaata
     9001 tggtggcact ttaaaacatt tagagccaga acaattgctt aaacctttat tccaacattt
     9061 taaagagaag tatccagagg taatatctaa aatagatgat gtagttttag gtaatgttgt
     9121 tgggaatggt ggcaatattg caagaaaagc attgcttgaa gcggggctta aagattcaat
     9181 acctggcgtc acaatcgatc ggcaatgtgg gtctggactt gaaagtgttc aatatgcatg
     9241 tcgcatgatc caagccggag ctggcaaggt atatattgca ggtggtgttg aaagtacaag
     9301 tcgagcacct tggaaaatca aacgaccgca ttctgtgtac gaaacagcat tacctgagtt
     9361 ttatgagcgt gcatcatttg cacctgaaat gagcgaccca tcaatgattc aaggtgctga
     9421 aaatgtggcc aagatgtatg atgtttcaag agaattacaa gatgaatttg cttatcgaag
     9481 tcatcaactg acagcggaaa atgtaaagaa tggaaatatt tctcaggaaa tattacctat
     9541 aaccgttaaa ggagaaatat tcaatactga tgaaagtcta aaatcacata ttccgaaaga
     9601 taactttggc cgatttaagc ccgtaatcaa aggtgggacc gttaccgctg cgaatagttg
     9661 tatgaaaaat gatggtgcag ttttattgct tattatggaa aaagatatgg catacgaatt
     9721 aggtttcgag catggtttat tatttaaaga tggtgttacg gtaggtgttg attctaattt
     9781 tcctggcatt ggtccagtac cagccatttc caacttacta aaaagaaatc aattaacgat
     9841 agaaaatatt gaagtcattg aaattaacga agcgttcagt gcacaggtag ttgcctgcca
     9901 acaagcttta aatatttcaa atacgcaatt aaatatatgg ggtggtgcat tagcatcagg
     9961 tcatccatac ggtgcaagcg gtgcccaatt agtgactcga ttattttata tgtttgacaa
    10021 agagactatg attgcatcta tggggatagg gggaggtcta ggaaatgcag cattatttac
    10081 tcgattctaa ccagcgatta aatgtgtcat tttctaagga tagtgtggct gcatattatc
    10141 agtgttttaa ccaaccttat agaaaagaag taccaccatt aatgtgtgcg tcattatggc
    10201 caaaatttga tttatttaaa aaatatgcaa atagcgaact gattttaaca aaatcagcaa
    10261 ttaatcaaac tcaaaagata gaagtagaca caatatatgt agggcattta gaagatattg
    10321 aatgccgaca gactcgcaat atcacacgtt atacaatggc tttaacatta actaaaaatg
    10381 atcaacatgt cataacggtt acacaaactt ttattaaggc gatgaagtag agatgaagtt
    10441 taatgagata tggataaatg aatatttggc gctcgtaaat gatgataatc caatacataa
    10501 tgagattgtg ccaggacaat tagtgagtca aatgatgctg atggctatgt cattagagac
    10561 aaaccagtgt caaattaact acgttaaacc tattttaata aatgaaaata tcgaattcat
    10621 tgaacaacac gaacacgaaa ttatagcaat taatgacgat ggagagatta aaataaaaat
    10681 ttctttgagc acaaaaaaat aaccgatatt agctgcatga acgcatatta attaggagat
    10741 gaaaggacag ctaatatcag ttatgtattg ttattattat tgggaacaga gatgaatata
    10801 ggttacgttt ctttctttgc acggggatgc attaatctaa aataataata acaactatat
    10861 caatgtttaa taaattctgg attattggaa cgattagtca atttaactaa ctttcatatg
    10921 atctatatcg tcttgtaata aagagagcaa tttgaatatt tcagtatcac taaatgaatc
    10981 gtcacattta attgaaacat gctgaaacgt tttggttata atttcataaa ctggtgcgcc
    11041 ttcatggtga tactgtcgat aaataatcat aacctatatt acctcctttg ctactctatg
    11101 gttatattat aaataacatt tttatgtgtg acatcaacct taagtatcaa ctttttatca
    11161 gacatagaac gtatgattta ctaagactat ttatgtataa aagttctaaa taaatatata
    11221 tttatagagt cgcctggcag tcatttggga aatataacat atatgattag agaggcatct
    11281 atcgcaaaag aatgataatg atagaggtat tgagcatata gatgagttta agttcatctt
    11341 gaaaataaag ggttatttag tcatagatgt agatgtatag gaaatatttg tatgtattgt
    11401 tcgatatgta tgaaattttc aataaaagct aataacgctt atatgtaact ttcaaattta
    11461 aattatatac agagcatgat gattataaaa aaataaccac atcacataaa ttgagttcat
    11521 acccaattta agtggtgtgg ctaataatgt tgatttatag atgaaccgcc taatcgttaa
    11581 acctctgtta cttcaacatc gatatgttca atacggttgt atgcaccgtg atccacagga
    11641 ccaacaaaat cattcatttt ccaaccgttt ttaatagcag aagcgacgaa agctttcgcg
    11701 ctaatcacag cttctttcgg tgacttaccg ttagctaaat atgcagttgt tgccgcagca
    11761 aatgtacaac cagcaccatg gttataactt tgttggaaca tgtctgttgt tagttgataa
    11821 aatgtttgac catcatagta taagtcatac gatttatctt gatctaaagc tttgccacct
    11881 ttaatgatga catgctgtgc gcctttatca aagataattg ttgcagcctt tttcatatct
    11941 tcaattgaat ttaatttacc taatcctgat aattgacccg cttcaaataa gtttggtgtc
    12001 actaccgttg ctttaggtag taaatattta atcatcgcct cagtatttcc aggattaagc
    12061 acttcatctt cgcctttaca aaccatgaca ggatctacta caaaatattg tgcattagat
    12121 gcctcatata cttctccagc acgtttgatt atctcctcag tacctaacat acctgtttta
    12181 atagcatcag gtccgattga taaagccgtt tcaagttgtt tttcaaatac atccattggt
    12241 aatggtgtaa catcgtgtga ccatgtatct ttatccatag taacgatggc agttaaagcg
    12301 accatgccat acgtatctaa ttcttggaac gttttcaaat ctgcttgcat acctgcgcca
    12361 gcacttgtgt cagaaccggc aattgttaaa actttcttta aagccattga gcttcactcc
    12421 tacataataa tattgtattc atcatatcat ttttaaccta attgaaaaat attaagcatt
    12481 caatatttga tgattgttga aatgaatcat tcatactatt gtaacttttg aaaatgtcat
    12541 tcactttaga taagtgtgat atgttaaaat atgtcctgag gtgagattga atggaatggt
    12601 cgcaaatttt tcatgacata acaacgaaac atgactttaa agctatgcat gattttttag
    12661 aaaaagaata ttcgactgca atcgtatacc ctgataggga aaatatatat caagcgtttg
    12721 atttaacacc gtttgaaaat atcaaagttg ttatattagg acaagacccg tatcatggtc
    12781 caaaccaagc acatggatta gcattttcag tgcaacctaa cgcaaaattc cctccatctt
    12841 tacgtaatat gtataaagaa ttagcagatg atattggatg cgttagacaa acaccgcatt
    12901 tacaagattg ggcaagagaa ggcgtcttgt tattgaatac agttttaacc gtaagacagg
    12961 gtgaagcaaa ttctcatcgt gatattggtt gggaaacatt tactgatgaa attattaaag
    13021 cagtgtctga ttataaagaa catgttgtct ttattttgtg ggggaaacct gcacagcaaa
    13081 aaataaagct tatcgataca tctaaacatt gtattataaa atcagtgcat cctagtccac
    13141 tgtctgcata tagaggattc tttggatcaa aaccgtattc caaagcgaat gcctatttag
    13201 agtcagtagg aaaatcacca attaattggt gtgaaagtga ggcgtagatg gtgaatagag
    13261 aaactttaat agcacgaatt gagcaagaat tagtacaagc agagcaggca cagcatgacc
    13321 atgactttga aaaacatatg tatgccatac atatattaac atctttatat gcttcaacat
    13381 caaatacacc acatattggt gaacaacaaa tgaatcgtcg tattgctaac cataatcaaa
    13441 tgccacaatc acaaataacg cagccaactc atcaagtgac agctgctgaa attgaagcga
    13501 tgggtggtaa agtaaatacg cattcagcac atcatcataa taagtcatat tcacaacctt
    13561 caaaccaaca acaaagatta gcgacagatg atgacattgg caatggtgaa tccatatttg
    13621 atttttaaaa agcaacaatg aaacataatt acttaatagc ttgttaagta tgttggttaa
    13681 taatcaagac gcatatactt ttattcgagt gttcggattt aaacatttat taatactgaa
    13741 ttatataagg agaggtagca atgaaattat ttattatttt aggtgcatta aacgcgatga
    13801 tggctgtcgg aacaggtgca tttggtgcgc atggtttaca aggaaaaata agtgatcact
    13861 atttatcagt atgggaaaaa gcaacgacgt atcaaatgta ccatggctta gcattattaa
    13921 ttataggtgt aattagtggt acaacttcaa tcaatgttaa ctgggctggc tggttaatat
    13981 ttgctggtat tattttcttt agtggatcat tatatatttt agtattaact caaattaaag
    14041 ttttaggtgc gattacgcca attggtggcg tattgttcat cattggctgg ataatgttaa
    14101 tcattgcgac attcaaattt gctggttaaa ttttaaaact ttagattacc tatgtaacta
    14161 aacattaaat ttttaataaa aataatcaag aaaaagagtt acaaactcat cttttgggta
    14221 tagaatacct tcgaggtgag tttttattta tggaaaaaaa gaataagcaa atagatagag
    14281 gcgatttaaa acaaaaccta tctgaaaagt ttgtatgggc gattgcatat ggttcatgta
    14341 tcggatgggg cgcattcatc ttaccaggag actggattaa gcagtcaggt ccgattgcag
    14401 catcaattgg tatagttatt ggtgcattat taatgatatt aattgcggtt agttatggcg
    14461 cattagtaga gagatttcca gtatcagggg gcgcgtttgc ctttagtttc ttaagtttcg
    14521 gcagatatgt gagtttcttc tcatcatggt ttttaacttt tggttatgtc tgtgtcgttg
    14581 ctttaaatgc gaccgcattc agtttactag ttaaattctt attgccagat gtcttaaata
    14641 atgggaaact atacaccatt gcgggctggg acgtttatat tacggaaatc attattgcga
    14701 ccgtattact acttgtattc atgctagtaa cgattcgtgg cgcaagtgta tctggatcat
    14761 tacaatatta tttctgtgtg gcgatggtaa tcgtcgtatt attgatgttc tttggttcat
    14821 tctttggtaa taattttgca cttgaaaatt tacaaccgtt agctgaacct agcaaaggat
    14881 ggttagtgtc tattgtggtt attgtatccg tggcaccatg ggcatatgtt ggatttgata
    14941 atattccaca aacagcagaa gagtttaact ttgcaccaaa caagacattt aagcttatcg
    15001 tgtacagttt attagcagca tcattaactt atgttgtcat gattttatac actggttggt
    15061 tatcaacaag tcatcaaagt ttaaatgggc agttgtggtt aacaggtgct gttacacaaa
    15121 cagcatttgg ttatattgga ttaggtgtat tagcaattgc aattatgatg ggtatattta
    15181 ctggtttaaa tggattcttg atgagttcaa gtcgcttgtt attttctatg ggacgttcag
    15241 gtattatgcc aacaatgttt agtaaattac atagtaaata caaaacacca tatgtcgcaa
    15301 tcatattcct agtaggtgtg tcgttaattg caccttggct aggaagaact gcattgactt
    15361 ggattgtaga tatgtcatct acaggtgtat ccattgccta ctttattaca tgtttgtctg
    15421 cagcgaaatt attcagttat aacaaacaaa gtaatacgta tgcaccggtt tacaaaacgt
    15481 ttgctattat cggctcattt gtatcattca ttttcttagc gttgttatta gtgccaggtt
    15541 ctcctgcagc actgactgca ccatcttata ttgcattgct tggatggtta atcatcggtt
    15601 taatattctt tgtgattcga tatcctaaat tgaaaaatat ggataatgat gaattaagtc
    15661 gcttgatttt aaatagaagt gaaaatgaag ttgatgatat gattgaagaa cctgaaaaag
    15721 aaaaaactaa ataataaaag aatcgcacaa tataccttct tcattcggag gcgtatcgtg
    15781 cgattttttg tattataaat tgacatttaa gacgaggcag ctgaacctta tatataattg
    15841 ctaagagttt gggctgagcc atttctaaca atatttataa tcgtttaaaa gatttcacga
    15901 acccagaaac aattaatttg gaaatttggt cggcgaataa taaacctaat gcgatggcgc
    15961 ctgcaataag tgttacttct aacatggtat tgattgctgt gctgaaattt aataagacta
    16021 aattttttgt agcatcgtat gctaatccac ctggtactaa tggaatgata cccgttacca
    16081 taaaaatgat ggcaggttct ttttgtttac gagccatata atgacttaac aagcctaatg
    16141 ctaaactacc aaagaaacta gagtatatag tgtgcacatt aaagccgttg aagaataagg
    16201 tgtaaaccat ccatccacat gtaccaacga aaccacatga tagatataat tttctaggtg
    16261 catcaaaaat gacgcagaag aacattgaag ctaaaaagct aaagataaag tttaagatcc
    16321 aaaacatagt ctgatactcc tatactaaaa ttaatacgct accaacgcca gcaccgatgc
    16381 caaacgcagt aaccaatgct tctaatgatt tcgttgtgaa catcaacatg tgtccaccaa
    16441 ataaatcttg tattgcgttt gttattaata caccaggaac aataggcatg actgccgcaa
    16501 tgataatagt tgccaagtca cctgttggga taagtgtatg tccaataacg gcgataatcc
    16561 caataactaa tgaaccaatg aattctggga taaactgtgc atgtaacttg cgatctaaaa
    16621 tctcagtgac taggtatcct agactacctg ctaatatcgc agttaaaaca tcaatcaatc
    16681 taccaccttg taaatataag aaactcattg caatcattgc tgcagcaaaa cctttaaagg
    16741 gaagactgct gtcacgctta gcaacatata ttttttcaag ttgtgttttt gcttcggcta
    16801 aagaaatttc attatttgta atttgacgcg aaattttatt agcttgcgaa atttttatta
    16861 agtttgtatc tcgagaggta attctaaata ttctaggaaa cgattccgaa tgtaacgtaa
    16921 actggatgac agtgtttgta acaaagctgt tactttcact gtaaccaagt ttttttgcaa
    16981 tacgtgtcat ggtatcttct acacgcgtac cttctgcacc agattctaat agtatgcgag
    17041 cagcaagcat gacaacgtct ttgataagta cctcttgttt gtattcttct gaatttatgt
    17101 ccatttcatc accattgttt ataagaattt aa
//
LOCUS       DAHHSC010000039        16811 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.039, whole genome shotgun sequence.
ACCESSION   DAHHSC010000039 DAHHSC010000000
VERSION     DAHHSC010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 16811)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16811)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16811
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.039"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            66..200
                     /gene="graF"
                     /locus_tag="NBI57_000982"
     CDS             66..200
                     /gene="graF"
                     /locus_tag="NBI57_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance-associated protein GraF"
                     /protein_id="HCD1821946.1"
                     /translation="MSNENQNKKAAEKAKEVEEKLKDKKEEKTEDINQTKQDIQDTLN
                     "
     regulatory      859..959
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            1036..1611
                     /locus_tag="NBI57_000983"
     CDS             1036..1611
                     /locus_tag="NBI57_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HCD1821947.1"
                     /translation="MSKGLKLSEILVTVLISVVFAVIYNLWWFVYNGVQATGLHLEQL
                     TNGVWFMAAIVCYLIIPKPGIALLAEFAAGAGETIIMGRFDIPTIVYAFIQGLACELV
                     FAIFKYQSRSVMVAMLAGFCTAIAAFPIDYFYGYLNEVAGWNLTLFIVFRLISGAVVA
                     GVLSYLLVKALDKTGVTKLFRPAAKEDYDNL"
     gene            1626..3026
                     /locus_tag="NBI57_000984"
     CDS             1626..3026
                     /locus_tag="NBI57_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCD1821948.1"
                     /translation="MLKVSDLRLKYPNGQRKIFDHLNITIQDKEKVLLLGPSGCGKST
                     LLNVLSGIVPNLIELPMKYDELIVDPLSGVIFQDPDSQFCMPKVYEELAFVLENRQVP
                     REDMDALIINALNMVNLNVTPETYIKDLSGGMKQKLAIVETILQQSKTLFLDEPTAML
                     DVQATEDLWTKLIELWEDQTVVIVEHKVKHIWNHVDRVILMDYNGNIIADECPEIILQ
                     KYVHLLSEYGVWHPRAWEFAPSRVDFPTTNSHLLQFKNGRIIRGKSTLLSFSDLEIGL
                     GEWITITGANGSGKTTLLESIMQLIKYQGDVYFENQRLTKIKHAAKHMYLVYQNPELQ
                     FITNSVYDEINIHFNHLSKDQSDDETIQLLKLLDLQNVKDQHPYELSIGQKRRLSVAT
                     ALSSKADIIFLDEPTFGLDSHNTFQLIKLFQKRINLGQSIVMVTHDDEIIERYPSRRL
                     KISDGALLDCDGDTNV"
     gene            3019..3825
                     /locus_tag="NBI57_000985"
     CDS             3019..3825
                     /locus_tag="NBI57_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HCD1821949.1"
                     /translation="MYEQWKVRQSFVDDVNIITKLFLGIILFFFIIFVHNFDFMLYIT
                     ILMLVFLLMFNGTQFKITAIFITISIGFALLSSLFMILYGNGSHTILKFGFIHITTES
                     LVRGLHVSLRTIAISFFGILIALTSQVVMIFYSLMQHLKVKSKVAYAFMAAIRMIPLM
                     ISSLIQLRRSLKMRYQMIDAANYRGLKRFKHLIIPLLSQNIRKAHQLSVAMESKGFRD
                     GPRTYYYNVPFSYKDIIFIIVLIAIITMAYYCSITLPITGIDDVRLGRVG"
     gene            complement(4092..5339)
                     /locus_tag="NBI57_000986"
     CDS             complement(4092..5339)
                     /locus_tag="NBI57_000986"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HCD1821950.1"
                     /translation="MNVLVIGAGGREHALAYKLNQSNLVKQEFVIPGNEAMTPIAEVH
                     TEISESNHQGILDFAKQQNVDWVVIGPEQPLIDGLADILRANGFKVFGPNKQAAQIEG
                     SKLFAKKIMKKYNIPTADYKEVERKKDALTYIENCELPVVVKKDGLAAGKGVIIADTI
                     EAARSAIEIMYGDEEEGTVVFETFLEGEEFSLMTFVNGDLAVPFDCIAQDHKRAFDHD
                     EGPNTGGMGAYCPVPHISDDVLKLTNETIAQPIAKAMLNEGYQFFGVLYIGAILTKDG
                     PKVIEFNARFGDPEAQVLLSRMESDLMQHIIDLDEGKRTEFKWKNESIVGVMLASKGY
                     PDAYEKGHKVSGFDLNENYFVSGLKKQGDTFVTSGGRVILAIGKGDNVQDAQRDAYEK
                     VSQIQSDHLFYRHDIANKALQLK"
     gene            complement(5361..6839)
                     /gene="purH"
                     /locus_tag="NBI57_000987"
     CDS             complement(5361..6839)
                     /gene="purH"
                     /locus_tag="NBI57_000987"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HCD1821951.1"
                     /translation="MKKAILSVSNKTGIVEFAKALTQLNYELYSTGGTKRILDEANVP
                     VRSVSDLTHFPEIMDGRVKTLHPAVHGGILADRNKPQHLNELSEQHIDLIDMVVVNLY
                     PFQQTVANPDVTMDEAIENIDIGGPTMLRAAAKNYKHVTTIVHPADYHEVLTRLRNDS
                     LDESYRQSLMIKVFEHTAEYDEAIVRFFKGDKETLRYGENPQQSAYFVRTSNAKHTIA
                     GAKQLHGKQLSYNNIKDADATLALVKKFDTPAAVAVKHMNPCGVGIGDTIEQAFQHAY
                     EADSQSIFGGIVALNRAVTPELAEQLHSIFLEVIIAPKFTDEALDILKQKKNVRLLEI
                     DMTIDSNEEEFVSVSGGYLVQDKDNYVVPKEEMKVVTEVAPTDEQWEAMLLGWKVVPS
                     VKSNAIILSNNKQTVGIGAGQMNRVGAAKIALERAIEINDHVALVSDGFFPMGDTVEL
                     AAQHGIKAIIQPGGSIKDQDSIDMANKHGIAMVVTGTRHFKH"
     gene            complement(6854..7420)
                     /gene="purN"
                     /locus_tag="NBI57_000988"
     CDS             complement(6854..7420)
                     /gene="purN"
                     /locus_tag="NBI57_000988"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HCD1821952.1"
                     /translation="MVKIAIFASGSGSNFENIVEHVESGKLENIEVTALYTDHQNAFC
                     IDRAKKHDIPVYINEPKQFDSKAAYEQHLVTLLNKDKVEWIILAGYMRLIGPDLLASF
                     EGKILNIHPSLLPKYKGIDAIGQAYHSGDTITGSTVHYVDCGMDTGEIIEQRQCDIRP
                     DDSKEQLEEKVKKLEYELYPSVIAKIVK"
     gene            complement(7423..8451)
                     /locus_tag="NBI57_000989"
     CDS             complement(7423..8451)
                     /locus_tag="NBI57_000989"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HCD1821953.1"
                     /translation="MSKAYEQSGVNIHAGYEAVERMSSHVKRTMRKEVIGGLGGFGAT
                     FDLSQLNMTAPVLVSGTDGVGTKLKLAIDYGKHDSIGIDAVAMCVNDILTTGAEPLYF
                     LDYIATNKVVPEVIEQIVKGISDACVETNTALIGGETAEMGEMYHEGEYDVAGFAVGA
                     VEKDDYVDGSEVKEGQVVIGLASSGIHSNGYSLVRKLINESGIDLASNFDNRPFIDVF
                     LEPTKLYVKPVLALKKEVSIKAMNHITGGGFYENIPRALPAGYAARIDTTSFPTPKIF
                     DWLQQQGNIDTNEMYNIFNMGIGYTVIVDEKDASRALKILAEQNVEAYQIGHIVKNES
                     TAIELLGV"
     gene            complement(8444..9928)
                     /locus_tag="NBI57_000990"
     CDS             complement(8444..9928)
                     /locus_tag="NBI57_000990"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HCD1821954.1"
                     /translation="MFNYSGLNEECGVFGIWNHPEAAQLTYMGLHSLQHRGQEGAGIV
                     VSDQNELKGERGLGLLTEAINDDQMERLKGYQHAIGHVRYATSGNKGIENIQPFLYHF
                     YDMSVGICHNGNLINAKSLRQNLEKQGAIFHSSSDTEVIMHLIRRSKAPTFEEALKES
                     LRKVKGGFTFAILTKDALYGAVDPNAIRPLVVGKMKDGTYILASETCAIDVLGAEFVQ
                     DIHAGEYVVINDKGITVKSYTHHTTTAISAMEYIYFARPDSTIAGKNVHAVRKASGKK
                     LAQESPVNADMVIGVPNSSLSAASGYAEEIGLPYEMGLVKNQYVARTFIQPTQELREQ
                     GVRVKLSAVKDIVDGKNIILVDDSIVRGTTIRRIVKMLKDSGANKVHVRIASPEFMFP
                     SFYGIDVSTTAELISASKSPEEIKDYIGADSLAYLSVDGLIESIGLDYDAPYSGLCVE
                     SFTGDYPAGLYDYEANYKAHLSHRQKQYISKNKHFFDSEGNLNV"
     gene            complement(9907..12096)
                     /gene="purL"
                     /locus_tag="NBI57_000991"
     CDS             complement(9907..12096)
                     /gene="purL"
                     /locus_tag="NBI57_000991"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HCD1821955.1"
                     /translation="MSKFIEPSVEEIKLEKVYQDMGLSDQEYEKVCDILGRQPNFTET
                     GIFSVMWSEHCSYKHSKPFLKQFPTSGEHVLMGPGEGAGVVDIGDNQAVVFKVESHNH
                     PSAIEPYQGAATGVGGIIRDIVSIGARPINLLNSLRFGELDNKQNQRLLKGVVKGIGG
                     YGNCIGIPTTAGEIEFDECYDGNPLVNAMCVGVINHDMIQKGTAKGVGNSVIYVGLKT
                     GRDGIHGATFASEELTEESESKRPSVQIGDPFVGKKLMEATLEAITFDELVGIQDMGA
                     AGLTSSSSEMAAKGGSGLHLRLEQVPTREPGISPYEMMLSETQERMLLVVEKGNEQKF
                     LDLFDKHELDSAVIGEVTDTNRFVLTYDDEVYADIPVEPLADEAPVYILEGEEKDYNT
                     SKNDYTHIDVKDTFFKLLKHPTIASKHYLYDQYDQQVGANTIIKPGLQASVVRVEGTN
                     KAIASTIDGEARYVYNNPYEGGKMVVAEAYRNLIAVGATPLAMTDCLNYGSPEKKEIY
                     QQLIDSTKGMAEACDILKTPVVSGNVSLYNETKGTSIFPTPVVGMVGLIENVNYLNDF
                     EPQVGDKLYLIGDTKDDFGGSQLEKLIYGKVNHEFESLDLSSEVEKGESIKTAIREGL
                     LSHVQTVGKGGLLITLAKLSAHYGLGLKSSIDITNAQLFSETQGRYVVSVKSGKTLNI
                     DNAIEIGLLTDSDNFKVTTPYTEISENVSDIKQIWEGAIAQCLTTQD"
     gene            complement(12089..12760)
                     /gene="purQ"
                     /locus_tag="NBI57_000992"
     CDS             complement(12089..12760)
                     /gene="purQ"
                     /locus_tag="NBI57_000992"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase I"
                     /protein_id="HCD1821956.1"
                     /translation="MKFAVLVFPGSNCDRDMFNAAIKSGVEAEYVDYRETSLSGFDGV
                     LIPGGFSFGDYLRSGAMASVAPIISEVKRLATEGKPVLGVCNGFQILTEIGLLPGALL
                     HNDSHLFISRNEELEIVNNQTAFTNLYEQGEKVIYPVAHGEGHYYCTDEIYQQLKANN
                     QIILKYVNNPNGSYDDIAGIVNEKGNVCGMMPHPERALETLLGTDSGVKLFEAMVKSW
                     REQHV"
     gene            complement(12762..13025)
                     /gene="purS"
                     /locus_tag="NBI57_000993"
     CDS             complement(12762..13025)
                     /gene="purS"
                     /locus_tag="NBI57_000993"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HCD1821957.1"
                     /translation="MKTIELHITLQPQVLDTQGQTLTRAVHDLGYAQVNDIRVGKVLY
                     MTVDEVSDEKVHNIITTLSEKLFANTVIEEYSYKVLDDEKENA"
     gene            complement(13025..13729)
                     /locus_tag="NBI57_000994"
     CDS             complement(13025..13729)
                     /locus_tag="NBI57_000994"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HCD1821958.1"
                     /translation="MTLLYEGKAKRIFSTNQENELRVEYKDEVTAGNGAKKDTMAGKG
                     RLNNQITSIIFKYLQENGIESHFIKQLSETEQLVKPVKIIPLEVVVRNIASGSITKRL
                     GFENGEVFREPLVEFFYKNDALNDPLITDDHVKLLNIASDEDIEILKSKALKINNVLK
                     QLMDAMNLKLVDFKIEFGKTETGQILLADEISPDTCRIWDKATNANFDKDVYRNNTGS
                     LIETYQIFLNKLEDLK"
     gene            complement(13733..14857)
                     /gene="purK"
                     /locus_tag="NBI57_000995"
     CDS             complement(13733..14857)
                     /gene="purK"
                     /locus_tag="NBI57_000995"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HCD1821959.1"
                     /translation="MNFNKLKFGATIGIIGGGQLGKMMAQSAQKMGYKVVVLDPSEDC
                     PCRYVAHEFIQAKYDDEKALNQLGQKCDVITYEFENISAQQLKLLCEKYNIPQGYQAI
                     QLLQDRLTEKETLKSAGTKVVPFISVKESTDIDKAIETLGYPFIVKTRFGGYDGKGQV
                     LINNEKDLQEGFKLIETSECVAEKYLNIKKEVSLTVTRGNNNQITFFPLQENEHRNQI
                     LFKTIVPARIDKTAEAKEQVNKIIQSIHFIGTFTVEFFIDSNNQLYVNEIAPRPHNSG
                     HYSIEACDYSQFDTHILAVTGQSLPNSIELLKPAVMMNLLGKDLDLLENEFNEHPEWH
                     LHIYGKSGRKDSRKMGHMTVLTNDVNQTEQDMYAKFEGSN"
     gene            complement(14844..15326)
                     /gene="purE"
                     /locus_tag="NBI57_000996"
     CDS             complement(14844..15326)
                     /gene="purE"
                     /locus_tag="NBI57_000996"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002465404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HCD1821960.1"
                     /translation="MKVAVIMGSSSDWKIMQESCNMLDYFEIPYEKQVVSAHRTPKMM
                     VQFASEARERGINIIIAGAGGAAHLPGMVASLTTLPVIGVPIETKSLKGIDSLLSIVQ
                     MPGGIPVATTAIGAAGAKNAGILAARMLSIQNPSLVEKLNQYESSLIQKVEDMQNELQ
                     "
     gene            15527..16387
                     /gene="folD"
                     /locus_tag="NBI57_000997"
     CDS             15527..16387
                     /gene="folD"
                     /locus_tag="NBI57_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HCD1821961.1"
                     /translation="MVAKILDGKQIAKDYRQGLQDQVEALKEKGFTPKLSVILVGNDG
                     ASQSYVRSKKKAAEKIGMISEIVHLEETATEEEVLNELNRLNNDDSVSGILVQVPLPK
                     QVSEQKILEAINPEKDVDGFHPINIGKLYIDEQTFVPCTPLGIMEILKHADIDLEAKN
                     AVVIGRSHIVGQPVSKLLLQKNASVTILHSRSKDMASYLKDADVIVSAVGKPSLVTKD
                     VVKEGAVIIDVGNTPDENGKLKGDVDYDAVKEIAGAITPVPGGVGPLTITMVLNNTLL
                     AEKMRRGIDS"
BASE COUNT         5221 a         3218 c         2515 g         5857 t
ORIGIN      
        1 ttaacgaaag tacaaccggg taagatttat atataaataa ttatccaatt tgggaggaat
       61 taatcatgtc taatgaaaat caaaataaaa aagcagcaga aaaagctaaa gaagttgaag
      121 aaaaattaaa agataaaaaa gaagaaaaaa cagaagatat taatcaaaca aaacaagata
      181 tccaagatac actaaattaa tttaaatccc cttacaactt ttagttgtta ggggatttta
      241 tgcatgtgct cggcgcatgt gttcctcttc ttccttacaa cttttagttg ttagggggtt
      301 ttatgcatgt gctcggcgca tgtatccctc ttttacctta caacttttag ttgttagggg
      361 attttatgca tgtgctcggt gcatgtatcc ctcttcttcc ttacaacctt tagttgttag
      421 gggctttttt gcttgcgctc ggtgcatgta tccctctttt accttacaac ttttagttgt
      481 taggggcttt tttgcatgtg ctcggcgcat gtatccctct tttaccttac aacttttagt
      541 tgttaggggc ttttttgcat gtgctcggcg catgtatccc tcttttaccc tacaaactat
      601 aattcgtaag ccattatgca cgggttcttc actgttgtta aagaggtatg attgataagt
      661 atccctcttc atttatcagc tcaatcttat tacatgtcac atatttttat atctttaatt
      721 actaacattc ctactacatc ttcaatttaa atccaatgct aatattataa attttaaatc
      781 gaattaaacg aaatttcatg aagacaacct attgacataa gtttacaaac gtcttacagt
      841 ataattaatt aaataaatcg cacacactag gggtgtttta tactgagatg aggcttgccc
      901 tcaaaccctt tgaacctgat ctagcttgaa ctagcgtagg aaagtgttac tatacatatg
      961 ttttactaat atatattgtg aacgcataac tttcctatgg atggttgtgc gtttttttat
     1021 taggaggatg taaaaatgtc aaaaggttta aagctatctg aaatactagt aaccgtactt
     1081 atttcagtag tattcgcagt tatttacaat ttatggtggt ttgtttacaa tggtgtgcaa
     1141 gctacaggcc ttcacctcga acaattaaca aacggggttt ggttcatggc agcgattgtc
     1201 tgctatctaa ttattccaaa accaggtatt gctttactgg ctgaatttgc tgcaggtgcc
     1261 ggcgaaacta tcattatggg tcgtttcgat attccgacaa tcgtttacgc tttcatacaa
     1321 ggtttagcgt gtgaacttgt ctttgcaatt tttaaatatc aatctcgttc agttatggta
     1381 gctatgttag caggtttttg tacagcaatc gcggctttcc ctatagatta tttctatggt
     1441 tatttaaatg aagttgcagg ttggaactta acattattca tcgtatttag attaattagt
     1501 ggtgcagtag tcgccggcgt cctatcttac cttttagtaa aagctttaga taagacaggc
     1561 gtaacaaaat tatttagacc agctgctaaa gaagattacg acaacttata agaggagcat
     1621 tcagagtgtt aaaagtaagt gatttacgat taaaatatcc aaatggtcag cgtaaaattt
     1681 tcgatcattt aaatatcact attcaagaca aagaaaaagt acttttactc ggtccttctg
     1741 gttgcggtaa aagtacactt ctgaatgtat taagtggtat tgttcctaat ttaattgaat
     1801 tacctatgaa atatgatgaa ctaatcgttg acccattaag tggcgttatt ttccaagacc
     1861 ctgatagcca gttttgtatg ccaaaagtat acgaagaact tgcattcgtt ttagaaaata
     1921 gacaagtacc acgtgaagac atggatgcgt taattatcaa tgctttaaat atggtcaatt
     1981 taaatgttac ccctgaaacg tatatcaaag atttaagtgg cgggatgaaa cagaaattgg
     2041 caattgttga aaccattctt caacaatcaa aaacattgtt tttagatgaa ccgacagcaa
     2101 tgttagatgt tcaagcaaca gaagatttat ggactaaact aattgaactt tgggaagatc
     2161 aaactgttgt aatcgttgaa cataaagtta aacacatctg gaatcatgtc gaccgcgtca
     2221 ttttgatgga ttataacgga aatatcattg ccgatgaatg tcctgaaatc atattacaga
     2281 agtatgttca tttactcagt gaatatggtg tgtggcatcc acgtgcatgg gaattcgcac
     2341 caagtcgtgt tgactttcca acaacaaact cacacttatt acaatttaaa aatggacgta
     2401 ttattcgcgg taaatcaaca ttgctctcat tctcagattt agaaattggt ctaggtgagt
     2461 ggattacaat tacaggggca aatggtagtg gtaaaacaac cttgcttgaa tcaattatgc
     2521 aattgattaa atatcaaggt gatgtttatt ttgaaaatca gcgtttaaca aaaattaaac
     2581 atgcagcaaa acacatgtac ctagtttatc aaaacccaga attacaattt ataacaaatt
     2641 cggtttatga tgaaattaac attcatttta atcacctttc taaagatcaa agtgatgatg
     2701 aaacgataca acttttaaaa cttttagatt tacaaaatgt aaaagatcaa catccttatg
     2761 agttgtctat tggtcaaaaa cgacgcctta gcgtagctac cgcactaagt tctaaagctg
     2821 atattatctt tttagatgaa ccgacatttg gacttgatag ccataataca ttccagttga
     2881 tcaaactttt ccaaaagcga attaatttgg gacaaagtat tgtcatggtt acacatgacg
     2941 atgaaataat tgaacgttat ccatcaagaa gacttaaaat ttcagatggt gcgttactag
     3001 attgtgatgg tgatacgaat gtatgagcaa tggaaagttc ggcaatcatt tgttgatgat
     3061 gtcaacatta taacgaaact atttcttgga attatactat ttttctttat tatatttgtg
     3121 cataatttcg attttatgct ttatatcact atcttaatgt tagttttctt attgatgttt
     3181 aatggtactc agtttaaaat tacagcaatt tttataacta taagtattgg ctttgcatta
     3241 ctctcttccc tgttcatgat tttatacggg aatggtagcc atacaatttt gaaattcgga
     3301 tttatccata ttacaacaga aagtttagtt cgtggtttac atgtatcatt aagaaccatt
     3361 gctatttcat ttttcggtat cttaatcgca cttacatctc aggttgtcat gatattttat
     3421 agtttaatgc aacatttaaa ggttaaatca aaagttgctt atgcattcat ggctgccatt
     3481 cgtatgatac ctttaatgat tagttcttta atacagcttc gcagatcttt aaaaatgcgc
     3541 tatcagatga tagacgcagc aaattataga gggttaaaga gatttaagca tttaattata
     3601 cctctattaa gccaaaatat tagaaaggca catcaattat cagttgcgat ggagtcgaaa
     3661 ggatttagag atggtcctcg tacttactat tacaacgtac ctttctcata taaagatata
     3721 atctttatta tagttttaat tgccattatt actatggcat attattgttc aatcacctta
     3781 cccatcacgg gcatagatga tgttcgtctt ggtcgtgtag gttaaatttt atccataatt
     3841 gtatagatac taaattagaa attcattgta tagaaaaaga aaaccattta ttccatacta
     3901 aacaaaagag gtgccacaaa catgttattt tgttcatcca aacatgttgt ggcgcctctt
     3961 ttgtattaca tatgcataat cacaaatttg gtacagaaat caatgtccta gactctacaa
     4021 ctaaaaaagc tatgaaaaaa actatcgtta tagatttctt catagctaat cttagtattt
     4081 tttaaatata cttatttaag ttgtagtgct ttattcgcaa tgtcatgacg atagaataaa
     4141 tggtcacttt gtatttgaga tactttttcg tatgcgtctc gctgtgcatc ttgtacattg
     4201 tcaccttttc cgatggcaag tataactcta ccacctgaag taacaaaggt atcaccttgc
     4261 ttctttaatc cactaacaaa atagttttca tttaaatcaa agccacttac tttatgccct
     4321 ttttcatatg catcaggata tccttttgat gccaacatga cccctacaat agattcattt
     4381 ttccatttga attcagtacg ttttccttca tctaaatcaa taatatgctg cattaaatca
     4441 ctttccatgc gacttaataa tacttgagct tcaggatcac caaaacgggc attaaattct
     4501 attacttttg gaccatcttt agttaaaata gcaccaatgt ataatacacc gaagaattga
     4561 taaccttcat taagcattgc ctttgcaatg ggttgtgcaa ttgtttcatt tgtaagtttt
     4621 aaaacatcgt cactaatatg tggtactgga caataagccc ccataccacc agtatttggt
     4681 ccttcatcat gatcaaatgc gcgtttatga tcttgtgcaa tacagtcgaa aggtactgct
     4741 aaatcaccat taacaaatgt cattagcgag aactcttcac cttctaaaaa cgtttcaaat
     4801 acaacagtac cttcttcttc atcaccatac ataatctcaa tagcacttct ggctgcttca
     4861 atagtatctg caataataac gcctttccca gcagctaacc catctttctt gacaacaacg
     4921 ggcaattcac agttttcaat atatgttaaa gcatcctttt ttcgctcaac ttctttataa
     4981 tcagcagttg gaatattata ttttttcatt atctttttag caaataattt tgagccttcg
     5041 atttgagctg cttgcttatt tggaccaaac actttgaaac cattcgctcg taaaatgtct
     5101 gctaatccat caattagcgg ctgttctgga cctataacta cccaatcaac attttgctgt
     5161 ttagcaaaat ctagtatccc ttgatgatta gattctgaaa tttcagtgtg tacttcagct
     5221 ataggtgtca ttgcctcatt acctggaatg acaaactctt gtttaactag attcgattga
     5281 ttaagtttat atgcaagtgc atgttctcgt ccaccagcac caattactaa tacattcatt
     5341 tatgttacgc ccctttaaaa ttagtgttta aaatgtcgag tgcctgtgac taccattgca
     5401 ataccatgtt tattagccat atcaattgaa tcttgatctt taatcgaacc acccggttgg
     5461 ataattgcct ttataccatg ttgtgctgca agttcaactg tatctcccat agggaaaaat
     5521 ccatcagata ctaacgctac atgatcattg atttcaatag ctctctctaa cgcaatttta
     5581 gcagcaccga cacgattcat ttgtccagca cctataccta cagtttgttt attattactt
     5641 aaaataattg cattactttt tactgatggt acaactttcc atcctaataa cattgcttcc
     5701 cattgttcat cagtaggtgc tacttctgta acaactttca tttcttcttt tggcacgaca
     5761 taattgtctt tatcttgaac taaatatccg ccagatactg aaacaaactc ttcttcgtta
     5821 ctgtctatag tcatatcaat ttctaataat cttacattct tcttttgttt taaaatatct
     5881 aatgcttcat ctgtaaattt tggtgcaata atgacttcca aaaagatact atgcaattgc
     5941 tctgctaact caggtgttac agctcggttt aatgcaacaa ttccaccaaa tattgattga
     6001 ctatccgctt catacgcatg ttgaaatgct tgttcaatcg tgtcaccgat accaacacca
     6061 catggattca tgtgtttaac cgcaactgca gcaggtgtat caaacttttt aactaaagct
     6121 agtgtagcat ctgcatcttt aatattgtta tagcttaatt gtttcccatg taattgttta
     6181 gcacctgcaa tcgtgtgctt agcattcgaa gttctcacaa aatacgctga ttgttgtgga
     6241 ttttctccat atcttaaagt ttctttatcc cctttaaaga aacgtacaat cgcttcatca
     6301 tattctgcag tatgctcaaa aactttaatc attaatgatt gtctatatga ctcatctaac
     6361 gaatcgtttc ttaatcgcgt caatacttca tgataatctg ccggatgtac aattgttgtt
     6421 acatgtttat agtttttagc tgcagcacgt aacattgttg gaccaccaat atcaatattt
     6481 tcaattgctt cgtccatcgt cacatcaggg tttgcaacag tttgttggaa tggatataaa
     6541 ttaactacta ccatatcaat taaatctata tgttgttctg ataattcatt taaatgctgc
     6601 ggtttatttc gatcagctaa aatgccacca tgaacagccg gatgtaatgt tttaacacgg
     6661 ccatccatta tttctggaaa atgtgttaag tctgaaacag aacgaactgg tacatttgct
     6721 tcatctaata tacgtttagt accaccagtt gaatataatt cataatttaa ttgcgttaac
     6781 gctttagcaa actctacaat acctgtttta tttgatacgc tcaaaatagc tttcttcatc
     6841 acatacatac tccttatttt acaattttag caataacact tggatataac tcatattcca
     6901 atttttttac tttctcttct aattgttctt ttgaatcgtc cggtctaata tcacattgtc
     6961 tctgttcaat aatttctccc gtatccatac cacaatcaac ataatgtact gtcgaaccag
     7021 taatagtatc gccactatga tatgcttggc ctattgcgtc aatcccctta tatttcggca
     7081 atagagatgg atgtatattc aatattttac cttcaaatga agctaataaa tctggaccaa
     7141 ttagacgcat gtagccagct aaaataatcc actctacctt atctttattt aataatgtta
     7201 ctaaatgttg ttcatacgct gcttttgaat caaattgttt tggttcatta atataaacag
     7261 gaatatcgtg cttttttgct ctatctatac aaaacgcatt ttgatgatcc gtatatagcg
     7321 ccgtaacttc aatattttca agttttcctg attcaacatg ctcaactata ttttcaaagt
     7381 tacttcctga acctgatgca aaaatcgcaa ttttaaccat tgttataccc ccaacaattc
     7441 aattgcagtt gactcatttt tcacaatatg accaatttga taagcttcca cattttgttc
     7501 tgctaaaatc ttcaaagcgc gtgatgcatc tttttcatca acgataaccg tatagccaat
     7561 acccatgtta aaaatgttat acatttcatt tgtgtctata ttgccttgtt gttgtaacca
     7621 atcaaatatt tttggcgttg gaaatgatgt agtatcaatt ctagcagcat atccggctgg
     7681 caatgcacgt ggaatatttt cataaaaacc tccaccagta atatgattca ttgccttaat
     7741 agaaacttct ttttttaaag caagtacagg tttgacatat aatttagttg gttctaaaaa
     7801 gacatctata aatggacgat tatcgaagtt tgatgccaaa tcaatgcctg attcattaat
     7861 taatttgcgc actaaactgt atccatttga atgaatgcca cttgacgcaa gtcctataac
     7921 aacttgtccc tctttcactt ctgaaccatc tacatagtca tccttttcaa ctgctccaac
     7981 agcaaatccg gctacatcat attcaccttc gtgatacatt tcacccattt cagcagtctc
     8041 tccaccgata agtgcagtat tcgtttcaac gcatgcatca ctaatacctt taacaatttg
     8101 ttcaataact tcaggaacaa ctttatttgt agcgatgtaa tctaaaaaat acaatggttc
     8161 tgcacctgtc gttaaaatat cattaacaca cattgcaact gcatcgatac ctatcgaatc
     8221 atgtttccca tagtcgatag ctagttttaa tttcgtacct actccgtctg ttccagaaac
     8281 taaaactggc gctgtcatat ttaattgtga taaatcaaat gtagcaccga atccacctaa
     8341 accaccgata acttctttac gcatcgtacg tttaacatga ctagacattc tttctacagc
     8401 ttcataacca gcatgaatat ttacaccaga ttgttcatat gctttagaca tttaaatttc
     8461 cctcgctatc aaaaaagtgt ttgtttttag aaatatattg cttttgtcga tgacttaaat
     8521 gtgctttata atttgcttca taatcatata accctgcagg ataatctcca gtgaaacttt
     8581 ctacacataa gccactatat ggcgcgtcat aatctaaacc aattgattca attaacccat
     8641 ctacagatag atatgctaat gaatcagcgc caatataatc tttaatttct tcaggtgatt
     8701 tgcttgcaga aattaattct gccgtagttg aaacatcgat tccataaaaa cttgggaaca
     8761 taaattccgg tgatgctata cgcacatgca ctttatttgc accagaatct tttaacattt
     8821 tcacaatgcg tcgaattgtc gtaccgcgaa caatggaatc atcaacaaga atgatatttt
     8881 tcccatctac tatatctttt accgcagata acttcactct cacaccttgc tcacgtaatt
     8941 cttgagttgg ttgaataaat gttcttgcaa catattgatt tttaactagt cccatttcat
     9001 atggcaaacc tatttcttca gcataaccac tcgcagctga tagcgatgaa ttgggtacac
     9061 cgatgaccat atcagcattt acagggcttt cttgggctaa ttttttacca gaagctttac
     9121 gtactgcatg gacattttta ccagctattg ttgagtctgg tctagcaaaa taaatatatt
     9181 ccatcgcaga aattgcagtt gtcgtatgat gtgtataaga tttaactgta atacctttat
     9241 cgttaatcac gacatattca cctgcatgaa tatcttgaac aaattctgca cctaacacat
     9301 ctattgcaca tgtttcactt gcaaggatgt atgtaccatc tttcatttta cctacaacaa
     9361 gtggtctgat agcatttgga tctactgcgc catataacgc atctttagtt aatatcgcaa
     9421 atgtaaaacc gcctttaact tttcgcaaac tttctttcaa tgcttcctca aaagtaggag
     9481 ctttacttcg acgtatcaaa tgcataatga cttcagtatc tgaagacgaa tggaagatag
     9541 caccttgttt ttctaaattc tgacgcaatg atttagcatt aatgaggtta ccattatgac
     9601 aaatacctac actcatatca taaaagtgat acagaaacgg ttgaatattt tcaatacctt
     9661 tattacctga agtagcataa cggacgtgac caattgcatg ttgatatcct tttaatcgtt
     9721 ccatttgatc atcgttaatc gcttcagtta gtaagcctaa tcctcgctcg ccttttaatt
     9781 cattttgatc agaaacaact atacctgcac cttcttgacc acgatgttgc aaactatgaa
     9841 gtcccatata tgttagttgc gctgcttcag gatgattcca aataccaaac acgccacatt
     9901 cttcgtttaa tcctgagtag ttaaacattg agcaattgcc ccttcccata tttgtttaat
     9961 atctgaaaca ttttcactaa tctctgtata tggtgttgtt actttgaaat tatcactatc
    10021 tgttaaaagt ccaatttcta ttgcattatc aatatttaaa gttttacctg atttaacaga
    10081 aacaacatat cggccttgtg tctcactaaa caattgtgca tttgttatat ctattgaaga
    10141 ttttaatcct aaaccgtaat gcgcacttag tttagctaag gtaatcagta agccaccttt
    10201 accaactgtt tgaacatgtg ataatagtcc ttcacgaata gcggtcttga ttgattcacc
    10261 tttttcaact tctgaactca aatctaatga ctcaaattca tgattaactt tgccataaat
    10321 taacttttca agttgactac caccaaagtc gtccttagta tcaccgatta aatataattt
    10381 atctccaact tgaggttcaa aatcatttaa ataatttaca ttttcaatca aacctaccat
    10441 tccaacaact ggtgttggga aaatagaagt acctttcgtt tcgttatata aagatacatt
    10501 tccagaaact actggtgtct taagaatgtc gcatgcttct gccatacctt tcgttgaatc
    10561 tatcaactgt tgatagattt ctttcttttc aggagaacca taatttaaac aatctgtcat
    10621 tgctaatggt gttgcaccca cggcaattaa atttcgataa gcttcagcta ctaccatctt
    10681 tccaccttca tatggattgt tatatacata acgcgcttca ccatcaattg ttgaagcaat
    10741 tgccttattt gtgccttcca cacgtactac cgatgcttga agtcctggct taattatcgt
    10801 attggcacca acttgttggt cgtattgatc atataaatag tgtttagatg ctatagtcgg
    10861 atgcttaagt aatttaaaga aagtatcttt aacatcgatg tgtgtataat catttttaga
    10921 agtattataa tctttttctt ctccttctaa aatatataca ggtgcttcat cagctagtgg
    10981 ttcaactgga atgtcagcat aaacttcatc atcatatgtt aaaacaaaac gatttgtatc
    11041 tgtaacttca cctataacag cactatccaa ttcgtgctta tcaaataaat ctaagaattt
    11101 ttgttcatta cctttttcaa caactagtaa catacgttct tgagtttctg aaagcatcat
    11161 ttcataagga gaaatacctg gctcacgtgt tggcacttgt tctaatctca aatgtaaccc
    11221 actaccacct tttgccgcca tttcagacga tgaagatgtt aaaccagcag cacccatatc
    11281 ttgaatacca actaattcat caaatgtaat tgcttcaagt gttgcttcca ttaatttttt
    11341 acctacaaat ggatcaccga tttgtacaga aggtcgttta ctttcgcttt cttccgtcaa
    11401 ttcttcagat gcaaaagtag caccatgaat accatctcga ccagttttca aaccaacata
    11461 aatgaccgaa ttacctacac cttttgctgt gcctttttga atcatgtcgt gattgataac
    11521 accaacacac attgcattaa caagtggatt gccatcataa cattcatcaa attcgatttc
    11581 accagcagtt gttggaatac caatgcagtt accataacct ccgataccct ttacaacccc
    11641 tttaagtaat ctttggtttt gtttattatc taattctcca aatctaagac tgtttaacaa
    11701 attaataggt ctagccccaa tagaaacaat gtcacgaatg attccaccaa cgcctgtagc
    11761 agccccttga tatggttcaa ttgctgatgg atgattgtga gactctactt taaatactac
    11821 ggcttgatta tcacctatat cgactacccc tgcaccttca cctggaccca taagcacatg
    11881 ctcacctgac gtaggaaatt gctttaaaaa cggtttagaa tgtttataag agcaatgttc
    11941 actccacata acagaaaaga tacctgtttc tgtaaagtta ggttgtctgc ctaaaatatc
    12001 gcaaactttt tcatattctt gatcacttaa tcccatatct tgatatactt tttcaagttt
    12061 aatttcttca acgcttggtt cgataaattt agacatgttg ttccctccaa ctttttacca
    12121 tcgcttcaaa taatttcaca ccactatcag tacctaacaa cgtttctaaa gctctttcag
    12181 gatgtggcat catgccacat acattgcctt tttcgttaac aattcctgca atatcatcat
    12241 atgaaccgtt cggattattc acatatttca gaataatttg attgttagct tttaattgtt
    12301 gatatatttc atcagtacaa taataatgac cttcaccgtg agctacagga tatataactt
    12361 tttcaccttg ctcataaaga tttgtaaatg ccgtttgatt attcactatt tctaactctt
    12421 catttctact aataaataaa tgtgaatcgt tatgcaataa tgcaccaggt aataagccta
    12481 tttcagttaa aatttgaaac ccattacaaa cacctaatac tggcttacct tcagttgcaa
    12541 gacgtttaac ttccgaaata atcggcgcta cactagccat tgccccagat cttaagtaat
    12601 ccccgaatga aaatccacca ggaataagta cgccatcaaa tccacttagt gatgtttctc
    12661 tataatctac atattccgct tcaacaccac ttttaatagc agcattaaac atgtctctat
    12721 cacaattcga acctggaaaa acaagaaccg caaatttcat tttatgcatt ctccttttca
    12781 tcatctaaca ctttatagct atattcttca atcactgtat ttgcaaacaa tttttcactt
    12841 agagttgtaa taatgttgtg taccttttca tcactaacct catccactgt catatataat
    12901 acttttccta cacgaatatc attcacttgt gcataaccta agtcatgtac agctcgagta
    12961 agcgtttgtc cttgcgtatc taatacttgt ggttgtaatg tgatatgtag ttcaattgtt
    13021 ttcattattt taaatcctcc aatttgttta aaaatatttg atatgtttca atcagtgatc
    13081 cagtgttatt tctatataca tctttatcaa agtttgcatt ggtagcttta tcccaaattc
    13141 gacatgtatc tggagatatt tcatccgcta acaaaatttg accagtctca gtctttccaa
    13201 attcgatttt aaaatctact aattttaaat tcatagcatc cattaattgt ttcaacacat
    13261 tattaatctt taatgctttg gattttagta tttcaatatc ttcatctgat gctatattga
    13321 gcaatttaac atggtcatcc gttatcaacg gatcatttaa cgcatcattt ttatagaaaa
    13381 attctacaag tggttctcta aaaacttcac cattttcaaa acctaaacgc tttgtaatag
    13441 atccactagc aatattacga acaactactt ctaatggaat tattttcaca ggcttaacta
    13501 attgttctgt ttcagataat tgtttaataa agtgactttc tattccattt tcttgtaaat
    13561 atttaaatat aatagaagta atttgattat ttaatcgccc cttacctgcc attgtgtctt
    13621 tcttagcccc gtttccagca gtaacttcat ctttatattc aactcttaat tcattttctt
    13681 gatttgttga gaaaatgcgc ttcgcttttc cttcatataa taatgtcatg ctttaattac
    13741 tcccctcaaa tttagcgtac atatcttgtt cagtttggtt tacatcattc gttagtacag
    13801 tcatatgccc catttttctg ctatctttac gcccagactt accataaata tgtaagtgcc
    13861 actctggatg ttcattaaat tcattttcca ataaatctaa gtctttacct agtaagttca
    13921 tcatgactgc aggctttaat aattcaattg aatttggtaa tgattgtccg gtaactgcta
    13981 aaatatgagt atcaaattgt gaataatcac atgcttcaat tgaataatgt ccggaattgt
    14041 gaggccttgg tgcaatctcg ttcacataca attggttgtt actatctata aaaaattcca
    14101 ctgtaaatgt tccaatgaaa tgaatcgatt ggataatttt attaacttgc tctttcgcct
    14161 cagctgtttt atctattctc gctggaacaa ttgttttgaa aagtatttga tttctatgct
    14221 cattttcttg taatgggaaa aaagtgattt gattgttgtt tcctcttgta acagtaagag
    14281 atacttcttt cttgatattc aaatattttt cagctacgca ttcactagtt tcaattaatt
    14341 taaaaccttc ttgtaagtct ttttcgttgt taattaaaac ttgacctttg ccatcgtagc
    14401 caccaaatct agtttttaca ataaaaggat atcctaatgt ttcaattgct ttgtcaatat
    14461 ctgtagattc ttttactgaa atgaacggga caactttggt accagcactt tttaatgttt
    14521 ctttttcagt taagcggtct tgtaataact gtatagcttg gtaaccttgc ggaatattgt
    14581 acttttcaca taatagtttt aattgttggg ctgaaatgtt ttcaaattca taagtaatca
    14641 catcacattt ttgtcctaat tgattgagtg ccttttcatc gtcatacttg gcttgtataa
    14701 attcgtgtgc aacgtatcta catggacaat cttcagaagg atccaataca accactttat
    14761 aacccatttt ttgagctgat tgtgccatca tctttccaag ctgaccacca ccaataatgc
    14821 caatagtcgc accaaacttt aatttattga agttcatttt gcatgtcctc cactttttga
    14881 attaacgaag attcatactg atttagtttt tcaactaaag aaggattttg aatacttaac
    14941 attcttgctg caagtatacc tgcgttttta gcacctgctg caccaattgc agtcgttgca
    15001 accggaatac ctcctggcat ttgaacaatt gataataaag aatctatacc ctttaaactt
    15061 tttgtttcaa tcggcacacc aataactggt agcgtcgtta atgatgcaac catacctggt
    15121 aaatgtgccg caccgccagc gcctgcaatg ataatgttta tacctctttc tctcgcttca
    15181 gaagcaaatt gaaccatcat ttttggcgta cgatgtgcgg atactacttg tttttcgtac
    15241 ggaatttcaa aataatccaa catgttacaa ctctcttgca taattttcca atcggaagaa
    15301 ctgcccataa tgactgctac tttcactttg tacacccttt caaaagtttg aattgtgaat
    15361 tactttagtt gtatattata gatatagcat aacaagcaat ttctgctttt tcaatcaaaa
    15421 atcgaacttt attttgattt tttgtttgaa tttacgtctt ttgctatgta aattagtttt
    15481 ataaactaac aaagttagga tattgacaat aggaggagaa gtttttatgg ttgctaaaat
    15541 tttagatggt aaacaaattg ccaaagacta cagacagggg ttacaagatc aagttgaagc
    15601 gctaaaagaa aagggtttta cacctaaatt atccgttata ttagttggta atgatggcgc
    15661 tagtcaaagt tatgttagat caaaaaagaa agcagctgaa aaaattggta tgatttcaga
    15721 aatcgtacat ttggaagaaa cagctactga agaagaagta ttaaacgaac taaatagact
    15781 aaataatgat gattctgtaa gtggtatttt ggtacaagta ccattaccaa aacaagttag
    15841 cgaacagaaa atattagaag caatcaatcc tgaaaaagat gtggacggat ttcatccaat
    15901 aaatataggg aaattatata tcgatgaaca aacttttgta ccttgcacac cgctcggcat
    15961 catggaaata ttaaaacacg ctgatattga tttagaagct aaaaatgcag ttgtaattgg
    16021 acgaagtcat attgtcggac aaccagtttc taagttacta cttcaaaaaa atgcatcagt
    16081 aacaatctta cattctcgtt caaaagatat ggcatcatat ttaaaagatg ctgatgtcat
    16141 tgtcagtgca gttggtaagc ctagtttagt aacaaaagat gtggtcaaag aaggagcagt
    16201 aattatcgat gttggcaata cgccagatga aaatggcaaa ttaaaaggtg acgttgatta
    16261 tgatgcggtt aaagaaattg ctggagctat tacaccagtt cctggtggcg ttggtccatt
    16321 aacaattact atggtattaa ataatacttt gcttgcagaa aaaatgcgtc gaggtattga
    16381 ttcgtaaaga gcctgagaca taaatcaatg ttctatgctc tacaaagtta taatggcagt
    16441 agttgactga acgaaaattc gcttgtaaca agcttttttc aattatagtc cggggcccca
    16501 acaaagaagc tgacgaaaag tcagcttaca ataatgtgca agttggggat gggccccaac
    16561 acagagaatt tcgaaaagaa attctacaga caatgcaagt cagcgaggcc ccaacaaaga
    16621 agctgtgact atgataaagt actactacat agttaatcat tagtggttct ttatcatttt
    16681 cgcctccctt ttcttattgt tttgatacac aaaaatttaa gttcaaactg tcgaataaag
    16741 ttatatttga tttcaaatta tccctaaatt attaatttta caattgtggc agattttcaa
    16801 aataataatt a
//
LOCUS       DAHHSC010000040        16406 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.040, whole genome shotgun sequence.
ACCESSION   DAHHSC010000040 DAHHSC010000000
VERSION     DAHHSC010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 16406)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16406)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16406
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.040"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            <1..1129
                     /gene="mnhD1"
                     /locus_tag="NBI57_000998"
     CDS             <1..1129
                     /gene="mnhD1"
                     /locus_tag="NBI57_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh1 subunit D"
                     /protein_id="HCD1821962.1"
                     /translation="GAFTTGDIFNLFVFFEVFLMSSYFLLVIGSTKIQLQETIKYVLV
                     NVVSSSFFVMGVAILYSVVGTLNLADISNKLANLSAHDSGLVNIVFILFIFVFATKAG
                     VFPMFVWLPSAYYAPPIPIIAFFGALLTKVGVYAIARTLSLFFSDNVSFSHYVILFLA
                     LLTIIFGCVGAVAYANIKKIILYNVMIAVGVILVGVAMMTESGMIGAIYYTLHDMLVK
                     LALFLLIGIMIKITGTADLRQFGGLIKRYPVLGWSFFIAALSLAGIPPLSGFYGKFFI
                     VQSTFERGFYLSGVIVLLSSLVVLYSVIRIFLQGFFGQPKGYDLNNKVDVKYLTTIAI
                     VAVVITVLYGLSADYLYPMVKAGAETFYNPSTYVKAVLGGK"
     gene            1131..1610
                     /gene="mnhE1"
                     /locus_tag="NBI57_000999"
     CDS             1131..1610
                     /gene="mnhE1"
                     /locus_tag="NBI57_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh1 subunit E"
                     /protein_id="HCD1821963.1"
                     /translation="MAVQLVLNFIIAVFWLFVTNSYTTNNFVLGFIFGLVLVYLLHRV
                     LPGRFYVITLYRIIKLVIIFLIELIKANFDVLKIIIKPSIKNEPGFFVYHTDLKKDWQ
                     IVLLSNLITLTPGTVVLGVSDDRTKIYIHAIDFSTKEQEVESIKTSLEKIVREVGEI"
     gene            1610..1903
                     /gene="mnhF1"
                     /locus_tag="NBI57_001000"
     CDS             1610..1903
                     /gene="mnhF1"
                     /locus_tag="NBI57_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh1 subunit F"
                     /protein_id="HCD1821964.1"
                     /translation="MNHNVIIVIALIIVVISMLAMLIRVVLGPSLADRVVALDAIGLQ
                     LMAVIALFSILLNIKYMIVVIMMIGILAFLGTAVFSKFMDKGKVIEHDQNHTD"
     gene            1881..2237
                     /gene="mnhG1"
                     /locus_tag="NBI57_001001"
     CDS             1881..2237
                     /gene="mnhG1"
                     /locus_tag="NBI57_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh1 subunit G"
                     /protein_id="HCD1821965.1"
                     /translation="MIKIILISLALIFVIIGALISALAAIGLLRLEDVYSRAHAAGKA
                     STLGAMSLLFGTFLYFIATQGFVNMQLIVAIIFVLITGPLSSHMIMKAAYNIKTPYTK
                     KTKVDEISEDLKDTKL"
     gene            2486..3640
                     /locus_tag="NBI57_001002"
     CDS             2486..3640
                     /locus_tag="NBI57_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HCD1821966.1"
                     /translation="MHWTIIGGGIQGTAIAQKLLSSGLTTDLLTIIDPHETFCQRFNS
                     YTNRIEMPYLRSPIVHHVHPQPFHLKQFAKQHQYTNAFYGPYQRPELTMFMNHIAHAS
                     KQYQLEDCLVQGLVQTLDKQEDKWHIKLEDGQIITTDCVVIAIGSTNIPFMPDILKDK
                     QNVNHIFEKEHDQVVYDKTDHIVGSGITAAHLALKLLNHDNDKKIHLWLNKDIEIHDF
                     DADPGWLGPKNMSSFLSTKSMPERNAIVQRERHKGSMPHELYLRLKKHIKNGRINVHK
                     TPITQISGGVINTENDSVPYQQIMVATGFEQDFMSQPLIKQLIQNYDAPINECNYPVI
                     SEKLEWIPNLFVAGCFADLELGPFGRNVMGGRKAAERIEQAFLKLQQYSA"
     gene            complement(3696..4070)
                     /locus_tag="NBI57_001003"
     CDS             complement(3696..4070)
                     /locus_tag="NBI57_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HCD1821967.1"
                     /translation="MTHLLETFEMSIDHQEDGLVVISMPVTDKVKQPFGYLHGGASIA
                     LGETACSLGSANLIDTTKFIPLGLEMNANHIHSAKDGRVTATAEIIHRGKSTHVWDIK
                     IKNDKEQLITVMRGTVAIKPLK"
     gene            complement(4089..5405)
                     /locus_tag="NBI57_001004"
     CDS             complement(4089..5405)
                     /locus_tag="NBI57_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter family protein"
                     /protein_id="HCD1821968.1"
                     /translation="MINAVVIAVILMIVLCLCRLNVVISLFISALVGGLISGMSIEKV
                     INVFGKNIVDGAEVALSYALLGGFAALISYSGITDYLVGKIINAIHAENSRWSRVKVK
                     VTIIIALLAMSIMSQNLIPVHIAFIPIVIPPLLSLFNDLKIDRRLIGLIIGFGLCFPY
                     VLLPYGFGQIFHQIIQSGFAKANHPIEFNMIWKAMLIPSMGYIVGLLIGLYVYRKPRE
                     YETRKISDSDNVTELKPYILIVTIVAILATFLVQTFTDSMIFGALAGVLVFFISRAYN
                     WYELDAKFVEGIKIMAYIGVVILTANGFAGVMNATGDIDELVKTLTSITGDNKLFSII
                     MMYVIGLIVTLGIGSSFATIPIIASLFIPFGASIGLDTMALIALIGTASALGDSGSPA
                     SDSTLGPTAGLNVDGQHDHIRDTCVPNFLFYNIPLMIFGTIAAMVL"
     gene            complement(5816..7291)
                     /locus_tag="NBI57_001005"
     CDS             complement(5816..7291)
                     /locus_tag="NBI57_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M17 family metallopeptidase"
                     /protein_id="HCD1821969.1"
                     /translation="MNFKLNNTLSNEINTLIIGIPEHLNQLERISFNHIDITESLERL
                     KHQHIIGSKVGKIYTTAFDVQDQTYRLITVGLGNLKARSYQDMLKIWGHLFQYIKSEH
                     IEDTYLLMDSFISKYDQLSDVLMACGIQSERATYEFDHYKSSKKAPFKTNLNLISESL
                     IELDFIHEGISIGQSINLARDFSNMPPNVLTPQTFAEDIVNHFKNTKVKVDVKDYDTL
                     VSEGFGLLQAVGKGSKHKPRLVTITYNGKDKDVAPIALVGKGITYDSGGYSIKTKNGM
                     ATMKFDMCGAANVVGIIEAASRLQLPVNIVGVLACAENMINEASMKPDDVFTALSGET
                     VEVMNTDAEGRLVLADAVYYANQYQPSVIMDFATLTGAAIVALGDDKAAAFESNSKVI
                     LNDILQISSKVDEMVFELPITATERASINHSDIADLVNHTNGQGKALFAASFVTHFSG
                     QTPHIHFDIAGPATTNKASYNGPKGPTGFMIPTIVQWLKQQ"
     gene            complement(7422..8630)
                     /locus_tag="NBI57_001006"
     CDS             complement(7422..8630)
                     /locus_tag="NBI57_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HCD1821970.1"
                     /translation="MAQDRKKVLVLGAGYAGLQTVTKLQKAISTEEAEITLINKNEYH
                     YEATWLHEASAGTLNYEDVLYPVESVLKKDKVNFVQAEVTKIDRDAKKVETNQGIYDF
                     DILVVALGFVSETFGIEGMKDHAFQIENVITARELSRHIEDKFANYAASKEKDDNDLS
                     ILVGGAGFTGVEFLGELTDRIPELCSKYGVDQNKVKITCVEAAPKMLPMFSEELVNHA
                     VSYLEDRGVEFKIATPIVACNEKGFVVEVDGEKQQLNAGTSVWAAGVRGSKLMEESFE
                     GVKRGRIVTKQDLTINGYDNIFVIGDCSAFIPAGEERPLPTTAQIAMQQGESVAKNIK
                     RILNGESTEEFEYVDRGTVCSLGSHDGVGMVFGKPIAGKKAAFMKKVIDTRAVFKIGG
                     IGLAFKKGKF"
     gene            complement(9084..9443)
                     /locus_tag="NBI57_001007"
     CDS             complement(9084..9443)
                     /locus_tag="NBI57_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly accessory protein"
                     /protein_id="HCD1821971.1"
                     /translation="MPTVILTEAAAYEVKDMLKANEMPDGYLKIKVNGGGCTGLTYGM
                     GAEEAPGENDEVLEYFGLKVLVDKEDAPVLNGTTIDFKQSLMGGGFQIDNPNAIASCG
                     CGSSFRTAKVAGNPENC"
     gene            complement(9456..9692)
                     /locus_tag="NBI57_001008"
     CDS             complement(9456..9692)
                     /locus_tag="NBI57_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YuzB family protein"
                     /protein_id="HCD1821972.1"
                     /translation="MNPIVEFCLSNMAKGGDYVFNQLENDPDVDVLEYGCLTHCGICS
                     AGLYALVNGDIVEGDSPEELLQNIYAHIKETWIF"
     gene            10009..11073
                     /locus_tag="NBI57_001009"
     CDS             10009..11073
                     /locus_tag="NBI57_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HCD1821973.1"
                     /translation="MKNLVLLGGGYGNMRIMSRILTTSLPQDYTVTLVDRMPFHGLKP
                     EFYALAAGTKSDKDVRMKFPNHPQVNTVYGEINDIDLDAQIVSVGNSKIDYDELIIGL
                     GCEDKYHNVPGAEEYTHSIQTLSKARDTFHSISELPEGAKVGIVGAGLSGIELASELR
                     ESRSDLEIYLYDRGPRILRNFPEKLSKYVAKWFAKNNVTVVPNSNINKVEPGKIYNCD
                     EPKDIDLVVWTAGIQPVEVVRNLPIDINSNGRVIVNQYHQVPTYRNVYVVGDCADLPH
                     APSAQLAEVQGDQIADVLKKQWLNEPLPDKMPELKVQGIVGSLGDKQGFAYIMDRTVT
                     GRLASILKSGVLWLYKYHNG"
     gene            complement(11132..11455)
                     /locus_tag="NBI57_001010"
     CDS             complement(11132..11455)
                     /locus_tag="NBI57_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YuzD family protein"
                     /protein_id="HCD1821974.1"
                     /translation="MEHVSVVVYGADVICASCVNAPTSKDIYDWLQPLLKRKYPNISF
                     KYTYIDITKDNDNLTDHDLQFIERIEQDELFYPLITMNDEYVADGYIQTKQITRFIDQ
                     KLVNE"
     gene            11556..11798
                     /locus_tag="NBI57_001011"
     CDS             11556..11798
                     /locus_tag="NBI57_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HCD1821975.1"
                     /translation="MPTEDTTMFDQVAEVIERLRPFLLRDGGDCSLIDVEDGIVKLQL
                     HGACGTCPSSTITLKAGIERALHEEVPGVIEVEQVF"
     gene            complement(12061..13236)
                     /gene="dltD"
                     /locus_tag="NBI57_001012"
     CDS             complement(12061..13236)
                     /gene="dltD"
                     /locus_tag="NBI57_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HCD1821976.1"
                     /translation="MKLKPFLPILISGAVFIVFLLLPASWFTGLVNEKTVEDNRTSLT
                     DQVLKGTLIQDKLYESNKYYPIYGSSELGKDDPFNPAIALNKHNANKKAFLLGAGGST
                     DLINAVELASQYDKLKGKKLTFIISPQWFTNHGLTNQNFDARMSQTQINQMFQQKNMS
                     TELKRRYAQRLLQFPHVHNKEYLKSYAKNPKETKDSYISGFKENQLIKIEAIKSLFAM
                     DKSPLEHVKPATKPDASWDEMKQKAVEIGKADTTSNKFGIRDQYWKLIQESKRKVRRD
                     YEFNVNSPEFQDLELLVKTMRAAGADVQYVSIPSNGVWYDHIGIDKERRQAVYKKIHS
                     TVVDNGGKIYDMTDKDYEKYVISDAVHIGWKGWVYMDEQIAKHMKGEPQPEVDKPKN"
     gene            complement(13233..13469)
                     /gene="dltC"
                     /locus_tag="NBI57_001013"
     CDS             complement(13233..13469)
                     /gene="dltC"
                     /locus_tag="NBI57_001013"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002433359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     2"
                     /protein_id="HCD1821977.1"
                     /translation="MEFREQVLNLLAEVAENDIVKENPDVEIFEEGIIDSFQTVGLLL
                     EIQNKLDIEVSIMDFDRDEWATPNKIVEALEELR"
     gene            complement(13487..14701)
                     /gene="dltB"
                     /locus_tag="NBI57_001014"
     CDS             complement(13487..14701)
                     /gene="dltB"
                     /locus_tag="NBI57_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PG:teichoic acid D-alanyltransferase DltB"
                     /protein_id="HCD1821978.1"
                     /translation="MIPYGDFTFFLIALIALLPVIILGFLGKRSYIYNGVVTAFMIVL
                     IFSSDKHNLFDQKYLSVQLISFIIYVVWQVLLIMFYYHSKPKNNSFSKFVTVMVLSIL
                     PLALVKVLQSTWLGGHQIHFHESKLIEFVGFLGISYVTFKSVQLIMEIRDGSIKEIKV
                     WKLIQFISFFPTISSGPIDRYKRFVKDDKKVPTGNEYRELVLKAIHMIMLGFLYKYIV
                     AYFINTYAIMPLQLDLHGFVNLWLYMYAYSLYLFFDFAGYSLFAIAFSYLFGIKTPPN
                     FDKPFKAKNIKDFWNRWHMTLSFWFRDCIYMRSLFYMSRKKLLKSQFAMSNVAFLINF
                     FIMGIWHGIEVYYIVYGLYHAALFIGYGYYERWRKKHPPRWQNGFTTALSIVITFHFV
                     TFGFLIFSGKLI"
     gene            complement(14698..16155)
                     /gene="dltA"
                     /locus_tag="NBI57_001015"
     CDS             complement(14698..16155)
                     /gene="dltA"
                     /locus_tag="NBI57_001015"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HCD1821979.1"
                     /translation="MTDIINKLQAFADANPQSIAVRHTTDELTYQQLMDESSKLAHRL
                     QGSKKPMILFGHMSPYMIVGMIGAIKAGCGYVPVDTSIPEDRIKMIINKVQPEFVFNT
                     TDESFESLEGEVFTIEDIKTSQDPVIFDSQIKDNDTVYTIFTSGSTGEPKGVQIEYAS
                     LVQFTEWMLELNKSGNKQQWLNQAPFSFDLSVMAIYPCLASGGTLNLVDKNMINKPKL
                     LNEMLTATPINIWVSTPSFMEMCLLLPTLNEEQYGSLNEFFFCGEILPHRAAKALVSR
                     FPSATIYNTYGPTEATVAVTSIQITQEILDQYPTLPVGVERLGARLSTTDDGELVIEG
                     QSVSLGYLKNDQKTAEVFNFDDGIRTYHTGDKAKFENGQWFIQGRIDFQIKLNGYRME
                     LEEIETQLRQSEFVKEAIVVPVYKNDKVIHLIGAIVPTTEVTDNAEMTKNIKNDLKSR
                     LPEYMIPRKFEWMEQLPLTSNGKIDRKKIAEVING"
     gene            complement(16171..16323)
                     /locus_tag="NBI57_001016"
     CDS             complement(16171..16323)
                     /locus_tag="NBI57_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499421.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HCD1821980.1"
                     /translation="MKSKSKQPPNKYVEAFKPYLLTLLYLAIFITLYLIYGSGDTHNN
                     FIYNEF"
BASE COUNT         5318 a         2889 c         2454 g         5745 t
ORIGIN      
        1 tggtgcattc acaactggtg acattttcaa cctattcgta tttttcgaag tatttttaat
       61 gtcatcttat ttcttattag taattggttc tacaaaaatt caactacaag aaacaatcaa
      121 atatgttctt gttaacgttg tatcgtcatc cttcttcgta atgggtgtag cgattttata
      181 ttcagtagta ggaacattga atctagcaga tataagcaat aaattagcta acctttcagc
      241 gcatgacagt ggcttagtca atatcgtttt catactattt atctttgttt ttgctacaaa
      301 agctggcgta ttcccaatgt ttgtttggtt acctagtgca tactatgcac caccaattcc
      361 aatcattgca ttctttggtg ctttgttaac aaaagttggt gtttatgcaa tcgctagaac
      421 attaagccta ttctttagtg ataatgtaag cttttcacat tatgtcattt tattcttagc
      481 actattaacg atcatctttg gttgtgtcgg tgctgttgca tatgccaaca ttaaaaaaat
      541 catcctatac aatgtcatga ttgctgtagg tgtgatctta gtcggcgttg caatgatgac
      601 agagtcaggt atgattgggg ctatttacta tacattgcac gacatgttag taaaacttgc
      661 attattcttg cttatcggaa ttatgattaa aattacaggc actgctgatt taagacaatt
      721 tggtggctta attaaacgat atcctgtact tggttggtca ttctttatcg cagcattaag
      781 tttagctggc attcccccac tcagtggctt ttatggaaaa tttttcattg tccaatcaac
      841 atttgaaaga ggattttatt taagtggtgt catcgtctta ttatcaagtt tagtcgtatt
      901 atattctgtc attcgcatat tcctacaagg ctttttcggt caaccaaaag gatatgatct
      961 taataacaaa gtcgacgtca aatatttaac tacgattgca atcgttgcag ttgtgataac
     1021 tgttttatat ggattgtcag cagattactt atatccaatg gtaaaagctg gtgcagaaac
     1081 attctacaat ccatcaacct acgttaaagc tgtattagga ggtaaataac atggctgtac
     1141 aattagtatt aaactttatt atcgcagtgt tttggttgtt tgtaacaaat agttacacaa
     1201 caaataactt tgtgttaggt ttcatctttg gcctagttct tgtttacctc ttacacagag
     1261 tgttaccagg tagattttat gtcatcacgc tatatcgtat tattaaatta gttattattt
     1321 tcttaatcga actgattaaa gctaattttg atgttttgaa aattattatt aaaccttcga
     1381 ttaaaaatga accaggattc tttgtttatc acacagattt aaaaaaagat tggcaaattg
     1441 tcttgctgtc taatttaatt acattaacac ctggtactgt agtgcttggt gtaagtgatg
     1501 atcgtacaaa aatttatatt catgccatag atttttcaac taaagagcaa gaagtagaaa
     1561 gtattaaaac atcattagag aaaattgtaa gagaggtggg cgaaatataa tgaatcataa
     1621 tgttattatc gttattgcat taatcatagt tgtcatttct atgttagcta tgctcattcg
     1681 cgttgtgcta ggcccatcac ttgccgatcg tgttgtcgca ttagatgcga ttggtcttca
     1741 attaatggca gttatagcat tattcagtat tttattaaat attaaataca tgattgtcgt
     1801 tattatgatg attggtatat tagccttttt aggtactgca gtattctcta aatttatgga
     1861 caaaggtaag gtgattgaac atgatcaaaa tcatactgat tagtcttgca cttatctttg
     1921 ttatcatcgg tgctttaatt agcgccctag cagctatagg attattgaga cttgaagatg
     1981 tatattcacg tgcacatgct gccggaaaag catcaacatt aggtgcaatg tcattactat
     2041 ttggtacttt tttatatttt attgctactc aaggttttgt aaatatgcaa ttaatcgttg
     2101 cgattatctt tgtattaatt acaggtcctc tttcaagtca tatgattatg aaagcagcat
     2161 ataatattaa aacgccttat actaaaaaga ctaaagtcga tgaaatttcg gaagacttaa
     2221 aagacacaaa attgtagatt atataacaaa atcatgaata taaatcaaca acaaacagca
     2281 gtaagatgat tccaaattag gaatgatttt actgctgttt tcttttgaca ttgttacctc
     2341 tttttcaatg attttttctt tgactacatc ttcgccctat ctacgtatat ctctttaatt
     2401 taattgcctt tcatgtcgtt atgtattatg ataataataa tcataaatcg taacgattac
     2461 gctttaaaaa aagagaggtt ttattatgca ttggacaatt atcggcggtg gcatacaggg
     2521 aactgcaatc gcacaaaaac tattatcaag cggattaaca acagacctat taacaatcat
     2581 tgacccacac gaaacttttt gccaaaggtt taactcatat acaaatcgaa tagaaatgcc
     2641 ttatttaaga tcaccgattg tacatcacgt acatccacaa ccgttccatc taaaacaatt
     2701 cgctaaacag caccaatata caaatgcttt ttatggtcct tatcaacgac ctgaattgac
     2761 aatgtttatg aatcatattg cacatgcttc taaacaatat caattagagg attgcttggt
     2821 tcaaggttta gttcaaactt tagataaaca agaagacaaa tggcatatca agttagaaga
     2881 tggacaaatt atcactacag attgcgtcgt tattgcaata ggtagtacaa atattccgtt
     2941 tatgcctgac attttaaaag acaaacagaa tgtaaatcat atcttcgaga aagaacatga
     3001 tcaagtagta tacgataaga ccgatcatat cgttggtagt ggcattactg ctgcacatct
     3061 tgcacttaaa ttgttaaatc atgataacga taaaaagatt catttatggc taaataaaga
     3121 tattgaaata catgactttg atgctgatcc tggttggtta ggtccgaaaa atatgtcttc
     3181 atttttaagt actaaaagca tgcctgaaag aaatgccatt gtacaacgtg aacgtcataa
     3241 aggatcaatg cctcacgaac tgtacttacg ccttaaaaaa catattaaaa atggtcgtat
     3301 aaatgtgcat aaaacaccta tcactcaaat tagtggtggt gtaattaaca ctgaaaatga
     3361 ttctgttcca tatcaacaga ttatggttgc aactggtttt gaacaagatt ttatgtcaca
     3421 accacttatt aagcaattaa tacaaaatta tgatgcacct atcaacgaat gtaattaccc
     3481 tgttatttcc gaaaaattag aatggatacc aaatctattt gtcgcaggat gctttgcaga
     3541 cttagaatta ggaccatttg gtagaaatgt tatgggtggc cgtaaagctg ccgaacgcat
     3601 tgaacaagca tttctaaaat tacaacaata tagcgcataa aaaaactgct agccgtagtt
     3661 atgaatatct cataacattt cagctagcag ttcttttatt ttaaaggttt aatagcaact
     3721 gtaccacgca taactgtaat taattgttct ttgtcattct taatttttat atcccataca
     3781 tgtgtcgact tacctcgatg aataatttca gctgtcgcag taacacgacc atctttagca
     3841 gaatgaatat ggttagcatt catctctaaa cctaatggaa taaatttggt tgtatcaatt
     3901 aaattagcag atcctaatga acatgctgtt tcacctaaag caatcgaagc cccaccatgt
     3961 aaatatccaa atggttgttt tactttatca gtgacaggca tagaaataac aactaaacca
     4021 tcttcctggt gatctattga catctcaaat gtctctaata aatgagtcat attttcacct
     4081 ctttatgctt atagtaccat agcagcaata gtaccgaaaa tcattaaagg aatattataa
     4141 aacaagaagt ttggtacaca tgtatcgcgt atatgatcat gttggccatc aacatttaat
     4201 cccgcagttg gtcctaatgt tgaatcactt gcaggtgaac ctgagtcacc taatgcactc
     4261 gctgttccaa tcaatgcgat taatgccatt gtatctagtc caattgacgc tccaaaagga
     4321 atgaataatg atgcgataat aggaattgtt gcaaatgatg atccaatacc taaagtgaca
     4381 attaaaccta tcacatacat catgataatg ctaaataatt tattatcacc agtaatactt
     4441 gttaaagttt taactaattc atctatatca ccagtagcat tcattacacc agcaaaccca
     4501 tttgctgtta aaataactac accaatataa gccataattt taataccttc aacaaactta
     4561 gcatctaatt cataccaatt atatgcacgt gaaataaaga atacgagtac ccctgccagt
     4621 gcaccaaaaa tcattgaatc tgtaaatgtt tgtactaaaa atgtagctag tattgccaca
     4681 attgttacta ttaagatata tggttttaac tctgtaacat tgtcactatc tgaaatttta
     4741 cgtgtttcat attcacgtgg tttacgatat acatataaac cgataagtaa gccaacaata
     4801 taccccattg aaggaataag cattgctttc caaatcatat taaactcaat tgggtgattc
     4861 gcctttgcaa agccactttg aataatttga tggaaaattt gaccgaatcc atatggtaat
     4921 aacacatacg ggaaacataa accaaaaccg ataatcaaac cgattaaacg tctatctatt
     4981 tttaagtcat taaacagact taacaatggt gggatgacaa ttggaatgaa tgcaatatgt
     5041 acaggaatta agttttgact catgatactc atagctaata atgcaatgat tattgtcact
     5101 tttactttaa ctcttgacca tcgactattt tcagcgtgaa ttgcatttat aatttttcct
     5161 actaaatagt ctgtgatacc actgtatgaa attaatgctg caaatccacc taataatgca
     5221 tagcttaatg ctacctcagc accatcgact atatttttcc caaatacatt tataactttt
     5281 tcaatgctca tgcctgaaat taagccacca actagcgcac tgataaataa gcttataact
     5341 acgtttaatc gacataaaca tagcacaatc attaatatta ctgctattac tactgcattt
     5401 atcatatgat aaccatcctt tatcgctcta tcgaactaaa gcgtttatag actacatagt
     5461 atcagtagct ttaacacctg tcaatagcaa actataccca taatctttat tttttagtta
     5521 attgtctttc ttaaaataat tttcactttc attaaattaa acaattttac aagcctggaa
     5581 caccaatcaa aatcctaagt tctataatgt tatattagta gtcgttgact gaatgagcat
     5641 atgattataa taagtttttg caatgctaat caagttgatt tatgctcaca aggatatgcg
     5701 atttatattt tcttacaaca atgaaaatgc ctgatacaat gcgatccttt atactttcaa
     5761 gagttaaatt agaaatgtat aacaagacac aagttgtatc aggcaaataa ttaaattatt
     5821 gttgttttaa ccattgtact atcgtcggaa tcataaatcc tgttggccct tttggaccat
     5881 tatatgaagc tttattagtc gttgctggac ctgcaatatc gaaatgaatg tgaggtgttt
     5941 gaccactaaa atgtgttaca aaacttgccg caaatagcgc tttaccttgt ccattcgtat
     6001 ggttaactaa atcagcgata tcactgtgat taatacttgc acgttcggtt gcagtaatcg
     6061 gtaattcaaa taccatttca tcgactttag aacttatttg taatatatcg tttaatatca
     6121 ctttactatt cgattcaaat gcagcagctt tatcatcgcc tagtgcaacg atggctgcac
     6181 ccgttaatgt agcaaagtcc ataatcacac taggctgata ttgatttgca taatacacag
     6241 catctgcaag gactaatcta ccttcagcgt ctgtattcat tacttctaca gtttcaccac
     6301 ttaatgctgt aaatacatca tctggcttca ttgatgcttc atttatcata ttttcagcac
     6361 aagcaagcac tccgacaata tttacaggca gttgtaaacg actagccgct tcaatgatac
     6421 caacgacatt cgcagcgcca cacatgtcaa acttcattgt agccatgcca ttcttcgttt
     6481 taatactata accaccagaa tcatacgtta tacctttacc aactaaggca attggtgcta
     6541 catctttgtc tttgccatta tatgtgatgg ttactaatct cggtttatgc ttactacctt
     6601 tacctactgc ttgtaaaagt ccgaatcctt cagaaactaa agtgtcataa tctttaacat
     6661 ctactttgac ctttgtattt ttaaaatgat taacaatatc ttctgcaaat gtttgtggtg
     6721 ttaatacatt cggtggcata ttactaaagt ctcttgccaa attaatggat tggccaatac
     6781 tgataccctc atgaatgaaa tctaattcaa ttaatgattc actaataagg tttaaattcg
     6841 tcttaaacgg tgccttctta cttgatttat aatgatcgaa ttcatatgtt gcacgctcac
     6901 tttgaatacc gcatgccatt aatacatctg ataactgatc atattttgaa ataaatgaat
     6961 ccataagtaa ataagtatct tcaatatgtt ctgactttat gtattggaaa agatgtcccc
     7021 atattttcaa catatcttga taactacgtg cttttaagtt tcctaaacca actgtaatta
     7081 aacgatatgt ttgatcttgt acatcaaatg cagttgtata aatcttccca actttactac
     7141 caataatatg ttgatgtttt agtctttcaa gtgattctgt aatatcgata tgattaaaac
     7201 taatgcgctc taactgattt aaatgttctg gtataccaat aatcaatgta tttatttcgt
     7261 tgcttagtgt gttatttaat ttaaaattca taatgtacct ccttagattt gatatgttac
     7321 tcacttcaaa ttgtacaaca ataaagccct tcagtgacac tgaaggactt tataaatgaa
     7381 taatttaatt ttatgtgttt agctgaaact ttatttaaag cctagaattt accttttttg
     7441 aatgctaaac cgataccacc gattttgaat accgcacgtg tatcaatcac ttttttcatg
     7501 aatgctgctt ttttaccagc gataggttta ccaaatacca tacctacacc gtcatgtgaa
     7561 cctaaagaac aaacagttcc acgatcaaca tattcgaatt cttcagttga ttcaccgttt
     7621 aagatgcgtt taatgttttt agcaacactt tcaccttgtt gcattgcaat ttgtgctgta
     7681 gttggtaatg gacgttcttc tccagctggg ataaacgctg aacagtcacc aataacaaaa
     7741 atgttgtcgt aaccattgat tgttaaatct tgctttgtaa cgatacgtcc acgtttaacg
     7801 ccttcaaatg attcttccat taatttacta ccacgtacac cagctgccca tactgaagta
     7861 cctgcattta attgttgttt ttcaccatct acttcaacta caaaaccttt ttcgttacaa
     7921 gcaacgattg gtgtagcaat tttaaattca acaccgcggt cttctaagta gctaactgcg
     7981 tggttaacta attcttctga gaacattggt aacattttag gtgctgcttc aacacaagtg
     8041 attttaactt tattttgatc cacaccatat ttgctacata attcaggaat tctgtctgtt
     8101 aattcaccta agaattcaac accagtgaat ccagcaccac caactaagat agataaatcg
     8161 ttatcatctt tttcttttga tgctgcatag ttagcaaatt tgtcttcgat atgacgtgat
     8221 aattcacgtg ctgtgataac attttcaatt tggaaagcat gatctttcat accttcgatg
     8281 ccgaatgttt cactaacgaa acctaatgct actactaaaa tatcaaagtc ataaatacct
     8341 tgatttgttt ctaccttttt agcatcacgg tcaatttttg ttacttctgc ttgaacaaag
     8401 ttcactttgt ctttcttcaa gacactttcc acaggatata atacatcttc atagtttagt
     8461 gtacctgctg atgcttcatg taaccatgtt gcttcatagt gatattcatt tttattaata
     8521 agcgtaattt ctgcttcttc tgttgatatc gctttttgca atttagttac agtttgtaaa
     8581 cctgcataac cagcaccaag tacaagtact tttttacgat cttgagccat ttaatttcac
     8641 ctaagctttc atattttttt aaccaaatgc tgataattac tagtaacaac tggtcattac
     8701 tacttttacc acaatgtata ctagtttatc atcattttgt tatttaagtc ttttaaatta
     8761 tgtaaaaacc tgtgatgttc acaaaaaaga catttttaat ccaatactaa ttctatagct
     8821 tttatgttat attttcaagc aagattcagt cagattgtga aaaaattaat taattttttc
     8881 gtagcctgat ttcgattaaa aatgtatccg ttatcacgat catttaaatt actatttata
     8941 gtcagattct taattttcaa taagcttttt aataaaacaa aatatcttta cgtaaaaata
     9001 ggacataatc aaatatgtcc ctaatcataa ttgatatcaa tttgtatccc aaaaattggg
     9061 ttaaccatta aaaagttatt ttattagcaa ttttcaggat tacctgcaac ttttgcagtt
     9121 ctaaatgaac taccacagcc acatgaagca attgcattag gattgtcgat ttggaaaccg
     9181 ccacccatta atgattgctt aaaatcaata gtcgtaccat ttaatacggg tgcatcttct
     9241 ttgtctacta atacttttaa tccaaagtat tctaagactt catcattttc accaggcgct
     9301 tcttctgcac ccataccgta tgttaaacca gtgcacccgc caccattcac tttaattttt
     9361 aaatagccat ctggcatttc atttgcttta agcatatctt ttacttcgta ggcagctgct
     9421 tctgttaata taactgttgg catgataact cctccttaaa aaatccatgt ttcttttata
     9481 tgtgcatata tattttgtaa taattcttcc ggcgaatcac cttcaacaat atcaccattt
     9541 actaaagcat acaacccggc tgaacatata ccacaatgtg tcaggcaacc atactctaac
     9601 acatcgacat ctgggtcatt ttccagttga ttaaaaacat aatctccacc ttttgccatg
     9661 ttagagagac aaaattctac gatcggattc atacttcacc ttcttatttc atttgttaca
     9721 atattatagc attttaaaac tggtatttta acatgatgtg ctcaattacc aacaactgat
     9781 gtttcttatc ccagttatgt aatagtgcct tagttagtac aaatatttat ttgtagtcat
     9841 tttcaaaaaa atatataata agcatataaa tacatattta cttttttagg ggttattgat
     9901 gaatcaaaga cacttgcatt ttataactgt tcattaacaa atatatctat gcactttgat
     9961 cttttcaaaa ttaaagtaaa tattaacata taaaaggggt acttcattat gaaaaactta
    10021 gttttgttag gcggcggata tggtaatatg cgtatcatgt cacgcatttt aactacttct
    10081 ttaccacaag attatacagt cacattagtt gatcgtatgc catttcatgg attgaaacca
    10141 gaattctatg ctttagctgc gggcacgaaa tcagataaag atgttcgcat gaaattccct
    10201 aatcatccac aagtgaatac agtttatggt gaaattaacg acatagattt agatgctcaa
    10261 attgtctcag tcggtaattc taaaattgat tatgatgagc taatcattgg tttaggatgt
    10321 gaagataagt atcataacgt tccaggagcc gaagaatata cacatagtat tcaaacactc
    10381 tcaaaggctc gggatacttt ccatagtatt agtgaactac cagaaggtgc taaagttggt
    10441 atcgttggtg ctggattaag tggcatagaa cttgccagcg aattaagaga aagtagatca
    10501 gacttggaaa tatatcttta tgaccgtggg ccgcgaattt taagaaattt tccagaaaaa
    10561 ttaagtaagt atgttgcgaa atggttcgcc aaaaataatg ttaccgttgt tccaaattca
    10621 aatattaata aagttgaacc tggtaaaata tataactgtg atgaacctaa agatattgat
    10681 ttagttgtat ggacagcagg aattcaacct gttgaagttg ttcgtaactt gccgattgat
    10741 ataaatagta atggacgcgt gatagttaac cagtatcatc aagtaccaac atatcgtaac
    10801 gtctatgtag ttggtgattg tgctgattta ccacatgcgc caagtgctca gttagccgaa
    10861 gttcaaggtg atcaaattgc cgatgtgctt aaaaagcaat ggctaaatga accattacct
    10921 gacaaaatgc cggaactaaa ggtacaaggt atcgttgggt cattaggaga taaacaaggt
    10981 tttgcatata tcatggaccg cactgtaaca ggacgactcg cttctatttt aaaatccggc
    11041 gtactttggt tatataaata tcataatggt taaaattaaa aagcaatgtc agttcacact
    11101 attactgaca ttgctttttg ttatgtgtat attattcatt cacaagcttt tggtctataa
    11161 atcgagtgat ttgctttgtt tgtatataac catctgctac atattcatcg ttcattgtaa
    11221 ttaatggata aaatagttca tcttgttcta ttctttcaat aaattgtaaa tcatgatctg
    11281 ttaagttgtc attatctttt gtaatatcta tatacgtata tttaaatgat atatttgggt
    11341 attttctttt taatagcggc tgtagccagt cataaatatc tttcgatgtt ggcgcattaa
    11401 cgcaacttgc acatataaca tctgccccat atactaccac actcacgtgc tccatatatt
    11461 tatcccccat tggtttgata gatttttatt acactatcta ttataatata ctaataaaga
    11521 ttatcaaatt catctctcga aaggagactt gcctgatgcc tactgaagat acaacgatgt
    11581 ttgatcaagt agcagaagtt attgaacgtc ttcgtccatt tttattacgt gatggtggcg
    11641 actgctcatt gattgacgtt gaagacggta ttgttaaatt acaattacat ggtgcatgtg
    11701 gtacatgccc aagttctaca atcactctta aagctggtat tgagcgtgca ttacacgaag
    11761 aagtgcctgg tgtaatagaa gtagaacaag tattctaata taaagatatt aagttaacag
    11821 aacatattat gaataacaac aaagacagga atactgaatt atttataatc agttattacc
    11881 tgtctttttt cgctgtcaaa ttggactgat gggaaagtta ttttatacta tttatctgat
    11941 taattatttt attattgcca catatccaag cacatattac tattctattc tatgaaattt
    12001 aaaatataaa aatagcacat acgctcaatc aaaatcgttg agttatgtgc tatttgtatt
    12061 ttaattttta ggtttatcta cttcaggttg tggttcacct ttcatatgtt tcgcaatttg
    12121 ctcatccata taaacccaac ctttccaacc gatgtgtacg gcatcactga taacatattt
    12181 ttcataatct ttatcagtca tatcgtaaat tttaccacca ttatctacaa cagtagaatg
    12241 gattttttta taaactgctt gacgacgttc tttatcgata ccaatgtggt cataccatac
    12301 accgtttgat ggaatactta catattgaac atctgcacca gcagcacgca ttgtttttac
    12361 aagtaattct aaatcttgga attctggaga attaacattg aattcgtagt cacgtctaac
    12421 tttacgctta ctttcttgaa ttaatttcca gtattgatct ctaataccaa atttattcga
    12481 tgtagtatca gctttaccaa tttcaactgc tttttgtttc atctcatccc aagaagcgtc
    12541 tggttttgta gcaggtttaa catgttctaa tggagattta tccattgcaa acaatgattt
    12601 aatcgcttct attttaatca attgattctc tttaaaacca gaaatataac tatctttagt
    12661 ttctttaggg tttttagcat aagatttcaa gtattcttta ttgtgtacat gtggaaactg
    12721 taataaacgt tgtgcataac gacgttttaa ttcagtagac atgtttttct gctggaacat
    12781 ttgattaatt tgagtttgag acatacgagc atcaaagttt tgattcgtta aaccatggtt
    12841 tgtaaaccat tgtggtgaaa taataaatgt taatttctta ccttttaatt tatcatactg
    12901 tgatgcaagt tcaactgcgt taattaagtc tgtagaacca ccagcaccta ataagaatgc
    12961 ttttttgttg gcgttatgct tatttaatgc aattgcagga ttaaatgggt catctttacc
    13021 taattcacta gagccgtata taggataata cttgtttgat tcgtataact tatcttgaat
    13081 gagtgtgcct tttagtactt gatctgtcaa tgaagttcta ttatcttcta cagtcttttc
    13141 atttactaat cctgtaaacc aactagcagg taataataga aagacaatga ataccgctcc
    13201 actaattaaa atgggtaaaa aaggttttaa tttcatcgta actcttctaa tgcttcaacg
    13261 attttatttg gtgttgccca ctcatctcta tcaaagtcca taatagatac ttcgatatca
    13321 agtttatttt gaatctctaa taataatcca actgtttgga aagaatcaat aataccttct
    13381 tcaaaaattt ctacgtctgg attttctttt acaatatcat tttctgctac ttctgctaat
    13441 aaatttaata cttgttctct aaattccata attaaattct cctttattat ataagtttac
    13501 ctgagaagat taaaaagcca aatgttacaa agtggaatgt aatcacaatg ctaagtgctg
    13561 ttgtgaaacc attttgccaa cgtggcggat gtttcttacg ccaacgttca taatagccat
    13621 aacctataaa caatgctgca tggtataaac cataaacaat gtaatacact tcgataccat
    13681 gccaaattcc cattatgaag aagttgatta agaatgccac gttagacatt gcaaattgac
    13741 tcttcaataa ttttttacga gacatgtaga ataaagatct catgtaaata caatctctga
    13801 accagaatga taatgtcata tgccatctat tccagaaatc tttaatattt ttcgctttga
    13861 aaggtttatc gaagtttggt ggtgttttaa taccgaataa ataactaaat gctatcgcaa
    13921 ataaactata acctgcaaag tcaaagaata aatataagct gtatgcgtac atatataacc
    13981 acaaattgac aaagccatgt aagtctaatt gtaacggcat gattgcatat gtgttaataa
    14041 agtaagcaac aatatattta tacaagaaac caagcataat catgtgaatt gcttttaata
    14101 ctaattcacg atattcattg cctgttggta cttttttatc gtctttaacg aaacgtttgt
    14161 aacgatcgat tggtccagat gaaatcgttg ggaagaatga aataaattga attaatttcc
    14221 atactttaat ttctttgata gaaccatcac gaatttccat aattaactgc acacttttga
    14281 atgtaacata agaaattcct aagaaaccaa caaattcaat taatttactt tcatggaagt
    14341 gaatctgatg tccacctaac catgtacttt gtaacacttt cacaagtgct aatggcaata
    14401 ttgataaaac cattacagtt acaaattttg aaaatgaatt attttttggt tttgaatgat
    14461 aataaaacat tatcaataaa acttgccata cgacgtaaat aataaaacta attaattgaa
    14521 cacttaaata cttttggtca aacagattat gtttatcaga agaaaagatt aacacaatca
    14581 taaatgctgt aactacgcca ttataaatgt aacttcgctt acctaaaaat ccaagtataa
    14641 tgactggtaa taatgcaatt aaagcaatta agaagaatgt aaaatcacca tatggaatca
    14701 tccgttaatt acctctgcaa ttttctttct gtcaatttta ccatttgaag tcaatggcaa
    14761 ttgttccatc cattcaaact ttctaggaat catatactct ggtaagcgtg atttcaagtc
    14821 atttttaata tttttagtca tttctgcatt gtccgtaact tcagtcgttg gcacaattgc
    14881 accaattaaa tgaataactt tatcattttt atatacaggt acaacaatcg cttcttttac
    14941 gaactcagac tggcgtaatt gtgtttcaat ttcttctaat tccattctgt agccattcaa
    15001 tttgatttgg aaatcaatac gaccttgaat gaaccattga ccattttcaa acttcgcttt
    15061 atcaccagtg tgatatgtac gaataccgtc atcgaaatta aatacttcag ctgttttttg
    15121 gtcatttttt aagtatccta aacttacact ttgaccttca ataacaagtt caccgtcatc
    15181 tgtagtagat aatcttgcgc ctagtctttc aacgccaaca ggtaatgtcg gatattgatc
    15241 taagatttct tgtgtaattt gaatacttgt aactgctacc gtagcttcag ttggaccata
    15301 tgtgttgtaa atcgtcgcac ttgggaaacg gctcactaac gcttttgctg ctctgtgagg
    15361 tagaatttca ccacagaaga agaattcgtt aagactacca tattgttctt cattaagcgt
    15421 tggtaataat aaacacattt ccataaatga tggtgttgat acccaaatgt taatcggtgt
    15481 tgctgttagc atttcattta ataatttagg tttattaatc atgtttttat ctacaagatt
    15541 taatgtaccg cctgatgcta aacatggata aatagccatt acagataaat caaatgaaaa
    15601 tggcgcttgg ttaagccatt gttgtttatt tcctgattta ttaagttcta acatccactc
    15661 agtaaattga actaaacttg catattcaat ttgaacacct ttaggttccc cagtagaacc
    15721 agatgtaaag attgtgtata ctgtgtcgtt atctttaatc tgactatcaa aaattactgg
    15781 gtcttgagat gttttaatat cttcaattgt aaatacttcg ccttctaaac tttcaaatga
    15841 ttcatcagtc gtattaaata caaactctgg ttgaaccttg ttaataatca ttttaatacg
    15901 gtcttcagga attgaagtgt ctacaggtac atatccacat cctgctttaa tggcaccaat
    15961 catcccaaca atcatatatg gtgacatgtg accgaataaa atcatcggtt tcttactacc
    16021 ttgtaatcga tgtgctaatt tactagactc atccattaac tgttgataag ttaattcatc
    16081 agttgtgtgt ctaacagcaa tgctttgtgg atttgcatcc gcaaacgctt gcagcttgtt
    16141 aataatatct gtcatattaa gtctccctca ttagaactca ttataaatga agttattgtg
    16201 tgtgtcgcca ctgccataaa ttaaatataa agtaataaat attgccaaat acaatagtgt
    16261 taataaatat ggtttgaatg cttcaacata tttattaggt ggctgtttac ttttagattt
    16321 catattgcac ctcttaaagt tcttagtaaa aacgccttta taaagaccgt tcaatataaa
    16381 atacgtttta aaatttgttt tttaca
//
LOCUS       DAHHSC010000041        16347 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.041, whole genome shotgun sequence.
ACCESSION   DAHHSC010000041 DAHHSC010000000
VERSION     DAHHSC010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 16347)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16347)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16347
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.041"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            complement(1023..1952)
                     /locus_tag="NBI57_001017"
     CDS             complement(1023..1952)
                     /locus_tag="NBI57_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HCD1821981.1"
                     /translation="MKKLVPLLLALLLLVAACGTGGKQSSDKSNGKLKVVTTNSILYD
                     MAKNVGGDNVDIHSIVPVGQDPHEYEVKPKDIKKLTDADVILYNGLNLETGNGWFEKA
                     LEQAGKSLKDKKVIAVSKDVKPIYLNGEEGNKDKQDPHAWLSLDNGIKYVKTIQQTFI
                     DNDKKHKADYEKQGNKYIAQLEKLNNDSKDKFNDIPKEQRAMITSEGAFKYFSKQYGI
                     TPGYIWEINTEKQGTPEQMRQAIEFVKKHKLKHLLVETSVDKKAMESLSEETKKDIFG
                     EVYTDSIGKEGTKGDSYYKMMKSNIETVHGSMK"
     gene            complement(1949..2785)
                     /locus_tag="NBI57_001018"
     CDS             complement(1949..2785)
                     /locus_tag="NBI57_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HCD1821982.1"
                     /translation="MLEFVEHLFTYQFLNRALITSIIVGIVCGTVGSLIVLRGLSLMG
                     DAMSHAVLPGVALSFLFGIPMFVGALITGMIASIFIGYITSSSKTKPDAAIGISFTAF
                     LASGIIIISLINTTTDLYHILFGNLLAITNSAFLTTIVIGSIVLILIIIFYRPLMIST
                     FDPTFSRMSGLNTTLLHYFVMLLLSLVTVASIQTVGIILVVALLITPASTAFLISKKL
                     YSMMIIASLISVISSIVGLYYSYIYNIPSGATIVLCTFVIYIITLFFTKFTNRKKRGS
                     LT"
     gene            complement(2779..3522)
                     /locus_tag="NBI57_001019"
     CDS             complement(2779..3522)
                     /locus_tag="NBI57_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HCD1821983.1"
                     /translation="MLETKDLNLFLGNKHVLKNISLSIPVRGEIIGIMGPNGAGKSSL
                     IKSLIGEFNATGTKLLYNKPIQQQLQHITYIPQKAHIDLDFPISVEQVILSGCYKEIG
                     WFRRPNKSARDKLKQLLSDLELESLRHRQISELSGGQLQRVLVARALMSESEVYFLDE
                     PFVGIDFSSEKLIMTKIENLKQQGKLILIIHHDLSKAKQYFDRIILLNQTLRYFGDSE
                     EAMSVTRLNETFMSSTDCSDPSQRSNITC"
     gene            3644..4288
                     /locus_tag="NBI57_001020"
     CDS             3644..4288
                     /locus_tag="NBI57_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HCD1821984.1"
                     /translation="MLTEEKEDYLKAILTNNGDKNFVTNKILSQFLNIKPPSVSEMVG
                     RLEKAGYVETKPYKGVRLTEDGLTHTLDIIKRHRLLELFLIEILKYNWEEVHQEAEIL
                     EHRISDLFVERLDSLLNFPETCPHGGVIPRNNEYKEKYITTILNYEPGDIVTIKRVRD
                     KTDLLIYLSSKDISIGNEVEIVSKDEMNKVIIIKRNDNVIIVSYENAMNMFAEK"
     gene            complement(4368..5120)
                     /locus_tag="NBI57_001021"
                     /pseudo
     CDS             complement(4368..5120)
                     /locus_tag="NBI57_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499199.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="M50 family metallopeptidase"
     gene            5356..6120
                     /locus_tag="NBI57_001022"
     CDS             5356..6120
                     /locus_tag="NBI57_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WecB/TagA/CpsF family glycosyltransferase"
                     /protein_id="HCD1821985.1"
                     /translation="MTVEERSNTAKVDILGVDFDNTTMLQMVENIKTFFANQSTNNLF
                     IVTANPEIVNYATTHQAYLELINQASYIVADGTGVVKASHRLKQPLAHRIPGIELMDE
                     CLKIAHVNHQKVFLLGATNEVVEAAQYALQQRYPNISFAHHHGYIDLEDETVVKRIKL
                     FKPDYIFVGMGFPKQEEWIMTHENQFESTVMMGVGGSLEVFAGAKKRAPYIFRKLNIE
                     WIYRALIDWKRIGRLKSIPIFMYKIAKAKRKIKKAK"
     gene            complement(6181..6975)
                     /gene="tagH"
                     /locus_tag="NBI57_001023"
     CDS             complement(6181..6975)
                     /gene="tagH"
                     /locus_tag="NBI57_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acids export ABC transporter
                     ATP-binding subunit TagH"
                     /protein_id="HCD1821986.1"
                     /translation="MNVSVNIKNVTKEYRIYRTNKERMKDALIPKHKNKTFFALDDIS
                     LKAYEGDVIGLVGINGSGKSTLSNIIGGSLSPTVGKVDRNGEVSVIAISAGLSGQLTG
                     IENIEFKMLCMGFKRKEIKAMTPKIIEFSELGEFIYQPVKKYSSGMRAKLGFSINITV
                     NPDILVIDEALSVGDQTFAQKCLDKIYEFKEQNKTIFFVSHNLGQVRQFCTKIAWIEG
                     GKLKDYGELDDVLPKYEAFLNDFKKKSKAEQKEFRNKLDEARFVIK"
     gene            7322..8134
                     /gene="tagG"
                     /locus_tag="NBI57_001024"
     CDS             7322..8134
                     /gene="tagG"
                     /locus_tag="NBI57_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acids export ABC transporter permease
                     subunit TagG"
                     /protein_id="HCD1821987.1"
                     /translation="MSAIGTVFKEHVKNFYLIQRLAQFQVKIINHSNYLGVAWELINP
                     VMQIMVYWMVFGLGIRSNAPIHGVPFVYWLLVGISMWFFINQGILEGTKAITQKFNQV
                     SKMNFPLSIIPTYIVTSRFYGHLGLLLLVIIACMFTGIYPSIHIIQLLIYVPFCFFLT
                     ASVTLLTSTLGVLVRDTQMLMQAILRILFYFSPILWLPKNHGISGLIHEMMKYNPVYF
                     IAESYRAAILYHEWYFMDHWKLMLYNFGIVAIFFAIGAYLHMKYRDQFADFL"
     gene            8233..9336
                     /locus_tag="NBI57_001025"
     CDS             8233..9336
                     /locus_tag="NBI57_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-glycerol glycerophosphotransferase family
                     protein"
                     /protein_id="HCD1821988.1"
                     /translation="MNVLIKKFYHLVVRILSKMITPQVIDKPHIVFMMTFPEDIKPII
                     KALNNSSYQKTVLTTPKQAPYLSELSDDVDVIEMTNRTLVKQIKALKSAQMIIIDNYY
                     LLLGGYNKTSNQHIVQTWHASGALKNFGLTDHQVDVSDKAMVQQYRKVYQATDFYLVG
                     CEQMSQCFKQSLGATEEQMLYFGLPRINKYYTADRATVKAELKDKYGITNKLVLYVPT
                     YREDKADNRAIDKAYFEKCLPGYTLINKLHPSIEDSDIDDVSSIDTSTLMLMSDIIIS
                     DYSSLPIEASLLDIPTIFYVYDEGTYDKVRGLNQFYKAIPDSYKVYTEEDLIMTIQEK
                     EHLLSPLFKDWHKYNTDKSLHQLTEYIDKMVTK"
     gene            9333..10394
                     /locus_tag="NBI57_001026"
     CDS             9333..10394
                     /locus_tag="NBI57_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HCD1821989.1"
                     /translation="MRLTIIIPTCNNEATIRQLLISIESKEHYRILCIDGGSTDQTIP
                     MIERLQRELKHISLIQLQNASIATCINKGLMDIKMTDPHDSDAFMVINPTSIVLPGKL
                     DRLTAAFKNNDNIDMVIGQRAYNYHGEWKLKSADEFIKDNRIVTLTEQPDLLSMMSFD
                     GKLFSAKFAELQCDETLANTYNHAILVKAMQKATDIHLVSQMIVGDNDIDTHATSNDE
                     DFNRYIIEIMKIRQRVMEMLLLPEQRLLYSDMVDRILFNNSLKYYMNEHPAVTHTTIQ
                     LVKDYIMSMQHSDYVSQNMFDIINTVEFIGENWDREIYELWRQTLIQVGINRPTYKKF
                     LIQLKGRKFAHRTKSMLKR"
     gene            10457..10855
                     /gene="tagD"
                     /locus_tag="NBI57_001027"
     CDS             10457..10855
                     /gene="tagD"
                     /locus_tag="NBI57_001027"
                     /EC_number="2.7.7.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate cytidylyltransferase"
                     /protein_id="HCD1821990.1"
                     /translation="MKRVITYGTYDLLHYGHIELLRRAREMGDYLIVALSTDEFNQIK
                     HKKSYYDYEQRKMMLESIRYVDLVIPEKGWGQKEDDVEKFDVDVFVMGHDWEGEFDFL
                     KDKCEVIYLKRTEGISTTKIKQELYGKDAK"
     gene            complement(10972..12267)
                     /gene="pbp4"
                     /locus_tag="NBI57_001028"
     CDS             complement(10972..12267)
                     /gene="pbp4"
                     /locus_tag="NBI57_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein PBP4"
                     /protein_id="HCD1821991.1"
                     /translation="MKNLISIIIILCLTLSIMTPYAQATNSDVTPVQAANQYGYAGLS
                     AAYEPTSAVNVSQTGQLLYQYNIDTKWNPASMTKLMTMYLTLEAVNKGQLSLDDTVTM
                     TNKEYIMSTLPELSNTKLYPGQVWTIADLLQITVSNSSNAAALILAKKVSKNTSDFVD
                     LMNNKAKAIGMKNTHFVNPTGAENSRLRSFAPTKYKDQERTVTTARDYAILDLHVIKE
                     TPKILDFTKQLAPTTHAVTYYTFNFSLEGAKMSLPGTDGLKTGSSDTANYNHTITTKR
                     GKFRINQVIMGAGDYKNLGGEKQRNMMGNALMERSFDQYKYVKILSKGEQRINGKKYY
                     VENDLYDVLPSDFSKKDYKLVVEDGKVHADYPREFINKDYGPPTVEVHQPIIQKANTV
                     AKSMWEEHPLFTIIGGACLVAGLALIVHMIINRLFRKRK"
     gene            12688..14415
                     /locus_tag="NBI57_001029"
     CDS             12688..14415
                     /locus_tag="NBI57_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCD1821992.1"
                     /translation="MKRENPLFFLFKKLSWPVGLIVAAITISSLGSLSGLLVPLFTGR
                     IVDKFSVSHINWNLIALFGGIFVINALLSGLGLYLLSKIGEKIIYAIRSVLWEHIIQL
                     KMPFFDKNESGQLMSRLTDDTKVINEFISQKLPNLLPSIVTLVGSLIMLFILDWKMTL
                     LTFITIPIFVLIMIPLGRIMQKISTSTQSEIANFSGLLGRVLTEMRLVKISNTERLEL
                     DNAHKNLNEIYKLGLKQAKIAAVVQPISGIVMLLTIAIILGFGALEIATGAITAGTLI
                     AMIFYVIQLSMPLINLSTLVTDYKKAVGASSRIYEIMQEPIEPTEALEDSENVLIDDG
                     VLSFEHVDFKYDVKKILDDVSFQIPQGQVSAFVGPSGSGKSTIFNLIERMYEIESGDI
                     KYGLESVYDIPLSKWRRKIGYVMQSNSMMSGTIRDNILYGINRHVSDEELINYAKLAN
                     CHDFIMQFDEGYDTLVGERGLKLSGGQRQRIDIARSFVKNPDILLLDEATANLDSESE
                     LKIQEALETLMEGRTTIVIAHRLSTIKKAGQIIFLDKGQVTGKGTHSELMASHAKYKN
                     FVVSQKLTD"
     gene            14772..16001
                     /locus_tag="NBI57_001030"
     CDS             14772..16001
                     /locus_tag="NBI57_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HCD1821993.1"
                     /translation="MFLLINIIGLIVFLGIAVLFSRDRKNIQWQSIGILVVLNLFLAW
                     FFIYFDWGQKAVRGAANGIAWVVQSAHAGTGFAFASLTNVKMMDMAVAALFPILLIVP
                     LFDILMYFNILPKIIGGIGWLLAKVTRQPKFESFFGIEMMFLGNTEALAVSSEQLKRM
                     NEMRVLTIAMMSMSSVSGAIVGAYVQMVPGELVLTAIPLNIVNAIIVSCLLNPVSVEE
                     KEDIIYSLKNNEVERQPFFSFLGDSVLAAGKLVLIIIAFVISFVALADLFDRFINLIT
                     GLIAGWIGIKGSFGLNQILGVFMYPFALLLGLPYDEAWLVAQQMAKKIVTNEFVVMGE
                     ISKDIASYTPHHRAVITTFLISFANFSTIGMIIGTLKGIVDKKTSDFVSKYVPMMLLS
                     GILVSLLTAAFVGLFAW"
     gene            16256..>16347
                     /locus_tag="NBI57_001031"
     CDS             16256..>16347
                     /locus_tag="NBI57_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1821994.1"
                     /translation="MFCYYKQHKGDNFSIEEVKNIIADNEMKVN"
BASE COUNT         5614 a         2404 c         2806 g         5523 t
ORIGIN      
        1 ccaccataaa atatgaagaa cctgaaaaac tgaagggctt ttcatcaatc ccttttattt
       61 taggggaatt gaatagatag ttttaaacta tacgaattat taatatttga gatttaattg
      121 aaataagttt taaaaattgg aggagataga ttaatcgaag tcatttaaag atgaagataa
      181 gtgcattcac aaaaaatagc cacactcata tgacatcgga tgagtgtggc ttaaggatct
      241 atggggggag gaaaccatag atgtttactt tgataggcca gattaaatat caaagtatgc
      301 gattatttat agcttgatgc aaaagtggta tgcctattaa aagttactgc acatagcttt
      361 taatattccg ttcaaaggaa aggggcatac aattgaacaa tctgtaatag tacttttaac
      421 cagctatgct aaaagtctag tagggagaac agttgtccaa tcacataaga acctctaact
      481 tcgttagtac gattaagaaa agctttttag ttagtatgta atacaattta ttgacgcgcg
      541 tgaatctctt ttataagagt gtgtagggaa tggcgttgta taaattgtat tagaagaact
      601 tctaacgcat ctctgtggtt aaaagagatg aagggaacga cagtttaatt aaaactgcat
      661 aagaacttct agcttttctc tttcgttcaa agagaagcag ctgttcgcag tttaatcaaa
      721 accacataaa gcttttaact ttactctttg atttaaagag tgacaaatgt ttacagttta
      781 attaaaactg cataagaact tctagctttt ctctttcgtt caaagagaag ttctaatacc
      841 accatatcgt gcgatcggga acggtatata tattaatagg agggtaatat atatttaacg
      901 cacgatatgg gactattagc cttcgacttt gttatgttga tgtgtagcct aaaatattgg
      961 agataccaat attttaggtt gcatcaacat caattcatct tacttcatta aaacacagcg
     1021 tgttatttca tgcttccgtg tacagtttca atatttgatt tcatcatttt gtagtaagag
     1081 tcacctttag tgccttcttt accgattgaa tctgtgtaca cttcaccaaa gatatctttc
     1141 ttcgtttctt cagataaact ttccattgct ttcttatcaa cacttgtttc tactaataag
     1201 tgttttaatt tgtgcttttt aacaaactca atagcttgtc tcatttgttc aggtgtacct
     1261 tgtttttcag tgttaatttc ccaaatataa cctggtgtaa taccgtattg ttttgagaag
     1321 tacttgaagg caccttcact tgtaatcatg gcacgttgtt cttttggaat gtcattaaat
     1381 ttgtctttac tgtcattatt taatttttcc aattgagcaa tgtatttgtt accttgcttt
     1441 tcataatctg ctttatgttt tttgtcgtta tcgataaatg tttgttgaat tgtttttacg
     1501 tatttaatac cattatctaa acttaaccat gcgtgtggat cttgtttatc tttgttgcct
     1561 tcttcaccgt ttaaatagat aggtttaaca tcttttgata ctgcgataac ttttttatct
     1621 tttaatgatt taccagcctg ttctaaggct ttttcaaacc aaccgttacc agtctctaaa
     1681 tttaatccgt tgtataaaat aacgtcagcg tcagttaact ttttaatatc tttaggttta
     1741 acttcatatt catgaggatc ttgaccaaca ggtacaatac tatgaatatc gacgttgtct
     1801 ccaccaacat ttttagccat atcatataaa attgaattcg tcgttactac ttttaatttg
     1861 ccatttgact tatcactgct ttgtttacca ccagtaccac atgcagcaac tagaagtaat
     1921 aaggctaata ataaaggtac taattttttc atgttaaact tcctcgtttc tttctattcg
     1981 taaattttgt gaaaaataat gtgatgatat aaattacaaa cgtacaaagt acgattgtcg
     2041 caccactagg aatgttgtaa atatagctgt aataaagtcc gacaattgaa cttatgacac
     2101 ttattaaact tgctataatc atcattgagt atagtttttt actaattaaa aatgctgtag
     2161 atgcaggtgt aattaataat gcaactacaa gaataatacc taccgtttga atacttgcta
     2221 ctgttactaa tgagagtaac aacatcacaa agtaatgtaa taacgtcgta tttagaccac
     2281 tcattctact aaacgttgga tcgaatgtag aaatcattaa tggacgatag aaaataatga
     2341 ttagaataag gacgattgaa ccaatcacaa tagttgttaa aaatgcacta tttgtgattg
     2401 ccagtaaatt accaaacaga atatggtaca aatctgtcgt agtgtttatt aagctaataa
     2461 taataatccc cgaagctaag aaagcggtaa aactaattcc aatagcggcg tcaggtttcg
     2521 ttttactact agatgtgata taaccgataa aaatacttgc gatcatacca gttataagtg
     2581 cgcctacaaa cattggaata ccaaataaga atgatagggc aacaccaggt aatactgcgt
     2641 gactcattgc atctcccatt aatgaaagac cacgtaatac aattaaacta ccaactgtac
     2701 cacaaactat ccctacaata attgaagtta tcaatgctcg attcaagaat tgatatgtaa
     2761 ataaatgttc gacaaactct aacatgttat attgctcctt tgactagggt cactacagtc
     2821 agtgctactc ataaatgttt cgtttaagcg agtgacactc atagcctctt cactatcacc
     2881 aaagtatcgt aatgtttgat ttaatagaat aatgcgatca aagtattgct ttgcttttga
     2941 tagatcatgg tggatgataa gaataagttt tccttgttgt tttaagttct cgatttttgt
     3001 catgattaat ttttcgctac taaaatcaat tccgacaaac ggctcatcta gaaaataaac
     3061 ttcactttcg gacatcaatg ctcttgctac tagcacacgt tgtaattgtc caccacttaa
     3121 ttctgaaatt tgtcgatgac gtaaagattc taattctaaa tcgcttaata actgtttgag
     3181 tttatccctt gctgatttat taggtcgtct aaaccatcca atttctttgt agcaacctga
     3241 taaaatcact tgttccacac ttataggaaa atctaaatca atatgtgctt tttgtggaat
     3301 atatgtaata tgttgcagtt gttgttgtat aggtttgtta tataacaatt tagtaccggt
     3361 agcattaaat tcaccaatta aagacttgat aagggaagat ttaccagcac cattcgggcc
     3421 catgatacca attatttcgc cgcgtactgg tatcgataag gaaatgtttt taagtacatg
     3481 cttattacct aaaaacagat ttaaatcttt tgtttctaac aaacgtttat acctcctaat
     3541 taaaagttta ggctaaccta attaattgta taataaactg agaatattta tcatgtcaag
     3601 taaattcgtg atataatata gacaatgtat gtgaggtgaa agtatgttaa ctgaagaaaa
     3661 agaggactat ttaaaggcaa tccttacgaa taatggcgat aaaaactttg tgacaaataa
     3721 aatcttatct caatttttaa atattaagcc tccatctgta agtgaaatgg taggacgtct
     3781 tgaaaaagca ggctatgttg aaacaaaacc atacaaaggt gttagattaa cagaggatgg
     3841 tttaacgcat acgcttgata tcattaagag acatcgacta ttagaattat ttttaataga
     3901 aatattgaaa tataattggg aagaagtaca tcaagaagca gaaattttag aacatcgaat
     3961 ttcagattta tttgttgaaa ggctggatag cctgttaaat ttcccagaaa cttgcccgca
     4021 cggcggtgtg attcctagaa ataatgaata taaagagaaa tatataacaa cgattttgaa
     4081 ttatgaacct ggtgatatcg ttacaatcaa acgtgtgaga gataagaccg atttgctaat
     4141 atatttgtct agtaaagata tttctattgg taatgaagtg gaaattgtat cgaaagatga
     4201 aatgaataaa gtaattatca ttaaacgtaa tgataatgta attattgtca gttacgaaaa
     4261 tgcaatgaac atgtttgctg aaaaataaaa taaagaagcc ataaagatat ccatgattga
     4321 actgataaag acatatggat aattgcttta ggcttctttt ttattagtta atttatcaag
     4381 tgagtatatt tgagtaaaat attcactgca taaagattga agataatcca gattgtacta
     4441 taaatgaaga taggtacatg actgagttct ttaagtgcac taccatccca ctgtggactc
     4501 ggacgctgga aagtcaattt agcaatcgtc caactagatt gtagaacttc gcctaataat
     4561 acacctaaaa tatattgata actcattgtg acaagtagtt gaatttctac tatattttca
     4621 tcttttaata taaaatacaa catgatagaa attaaagtta taacaacaat gggtgagcct
     4681 tttctagatg ttaaaattaa aaaataaata aatatcaata aataggtaaa tataaagaaa
     4741 ctaggtatct gataatggct cgacgctaaa cctatcaata acataatagg tggcattaaa
     4801 taaccaccaa tcgttgtaag ccattggcct gctagatgtc tagattgtgt aattgcgaat
     4861 ccttattgta atgtctgttg tcgctctcgt ggacttgtta caatgactaa atcttttgca
     4921 cggccaccag cgagtttatt aaacagtaca tgaccaaatt catgtgttaa aacagggata
     4981 tagtttaaaa tgacatctaa atagttcaaa acaggcttat gtctatattg atgaatagca
     5041 atataacaag ctgcaacaat aacgataatg tatatattaa gttgaattgt cgtattaaaa
     5101 aagtttgata aataattcat tgttaacctc atataagata ttaatttaaa gtttgcttag
     5161 cacttattat aaatgatatt ggcatcaata gcgttagact ttagacttac cttagttaaa
     5221 ctaattttaa tttttgaaaa ggtgaatatg tgttaaaata aagcaaaatc atttcgatat
     5281 aaataggatg aatataaata ctgttaatat tgattacact aacataataa tgaaataaga
     5341 taggagattc ctgttatgac tgttgaagaa agatccaata cagccaaagt tgacatttta
     5401 ggggtcgatt ttgataatac aacaatgttg caaatggttg aaaatattaa aacctttttt
     5461 gcaaatcaat caacgaataa tctttttata gtaacagcca accctgaaat agtgaattac
     5521 gcgacgacac atcaagcgta tttagagtta ataaatcaag cgagctatat tgttgctgat
     5581 gggacaggag tagtcaaagc ttcgcatcgt ttaaagcaac ctctagcgca tcgtatacct
     5641 ggtattgagt tgatggatga atgtttgaaa attgctcatg taaatcatca aaaagtattt
     5701 ttgctagggg caactaatga agttgtagaa gcggcacaat atgcattgca acaaagatat
     5761 ccaaacatat cgtttgcaca tcatcacggt tatattgatt tagaagatga gacagtagta
     5821 aaacgaatta aactgtttaa acctgattac atatttgtag gtatgggatt ccctaaacaa
     5881 gaagaatgga ttatgacaca tgaaaaccaa tttgaatcta cagtgatgat gggcgtaggt
     5941 ggttctcttg aagtatttgc tggggctaaa aagagagcgc cttatatatt tagaaaatta
     6001 aacattgaat ggatatatag agcattaata gattggaaac gtattggtag attgaagagt
     6061 attccaatat ttatgtataa aatagccaaa gcaaaaagaa aaataaaaaa agcgaaatag
     6121 tcatgatgat aaaaataaaa ccgaggaaat ccttaaatgg agattctcgg ttttttcggt
     6181 ttatttaata acgaagcggg cctcatcgag tttgtttcta aattcttttt gttcggcttt
     6241 ggatttcttt ttaaaatcgt taaggaaagc ttcatattta ggtaatacat catcaagttc
     6301 accgtaatct tttaactttc cgccttcaat ccaagcaatc ttagtacaaa attgtctcac
     6361 ttggcctaag ttatgactaa cgaaaaagat ggttttgttt tgctctttaa actcgtaaat
     6421 tttatctaaa catttttgtg caaaagtttg gtcacctaca gataaagctt cgtcaatgac
     6481 taagatatct ggattaactg tgatattaat tgaaaaacca agttttgcac gcataccact
     6541 tgaatacttt ttaactggtt gataaataaa ctcaccaagt tcactaaatt caataatctt
     6601 aggtgtcatc gctttaattt cttttcgctt aaagcccata cataacattt taaattcgat
     6661 attttcaatc cctgtaagtt gtccactcaa gccagcacta attgcgataa cgctgacttc
     6721 accattacga tccactttgc caacagtagg cgacaaagaa ccgccaatga tattgctcaa
     6781 cgttgatttg ccggaaccat tgatgccaac aagccctatg acgtcacctt catatgcttt
     6841 taaactaatg tcatctaaag cgaaaaatgt tttgttttta tgtttgggaa tgagcgcatc
     6901 tttcatacgt tctttatttg tacgataaat acgatattct tttgttacat ttttaatgtt
     6961 taccgaaacg ttcatttgta gaccttcctt attcacattt atctagatta taatatacta
     7021 ctcaacagtt gttaaatttt aaaacctgtt gtaaagtgta tagaagattt tgttattatc
     7081 agagttggtg ttttgacaca aaatgttaat catcaatgat aacaatgata tttaaaaact
     7141 aaacttattt caacttacat gattgtatac tataatgtat ttgtaataaa ctaatatttt
     7201 aaagaactag acaataattt tgatagcatc catgtatagt gatagtattt acaacaatta
     7261 ttataatact atttagttaa gtagagaaat agttaaacat ttgaaagtgt ggtttaatgg
     7321 aatgtcagca ataggaacag tttttaaaga acatgtaaag aacttttatt taattcaaag
     7381 actggctcag tttcaagtta aaattatcaa tcatagtaac tatttaggtg tggcttggga
     7441 attaattaac cctgttatgc aaattatggt ttactggatg gtttttggat taggaataag
     7501 aagtaatgca ccaattcatg gtgtaccttt tgtttattgg ttattggttg gtatcagtat
     7561 gtggttcttc atcaaccaag gtattttaga aggtactaaa gcaattacac aaaagtttaa
     7621 tcaagtatcg aaaatgaact tcccgttatc gataataccg acatatattg tgacaagtag
     7681 attttatgga catttaggct tacttttact tgtgataatt gcatgtatgt ttactggtat
     7741 ttatccatca atacatatca ttcaattatt gatatatgta ccgttttgtt ttttcttaac
     7801 tgcctcggtg acgttattaa catcaacact cggtgtgtta gttagagata cacaaatgtt
     7861 aatgcaagca atattaagaa tattatttta cttttcacca attttgtggc taccaaagaa
     7921 ccatggtatc agtggtttaa ttcatgaaat gatgaaatat aatccagttt actttattgc
     7981 tgaatcatac cgtgcagcaa ttttatatca cgaatggtat ttcatggatc attggaaatt
     8041 aatgttatac aatttcggta ttgttgccat tttctttgca attggtgcgt acttacacat
     8101 gaaatataga gatcaatttg cagacttctt gtaatatatt tatatgacga aaccccgcta
     8161 accattaata aatggaagtg gggttcattt ttgtttataa tttaagtaaa taacatatta
     8221 agttggtgta ttatgaacgt tttaataaag aaattttatc atttggtagt tcgaatactt
     8281 tctaaaatga ttacgcctca agtgattgat aaaccgcata tcgtatttat gatgactttt
     8341 ccagaagata ttaagcctat catcaaagca ttaaataatt cgtcgtatca gaaaactgtt
     8401 ttaacaacac caaaacaagc gccttattta tctgaactta gcgacgatgt tgatgtgata
     8461 gaaatgacta atcgaacatt ggtaaaacaa attaaggctt tgaaaagcgc gcagatgatt
     8521 attatcgata attattacct attactaggt ggatataata agacttctaa tcaacacatt
     8581 gttcaaacgt ggcatgcaag tggtgcatta aaaaactttg gattaacaga tcatcaagtc
     8641 gatgtgtctg acaaggcaat ggttcagcag taccgtaaag tttatcaagc gacggatttt
     8701 tacttagtgg gttgtgaaca aatgtcacaa tgttttaaac agtctttagg tgcaacagaa
     8761 gagcaaatgc tgtattttgg gcttccgaga attaataaat attacacagc tgatagagca
     8821 acggttaagg cagagttaaa ggataaatat ggaattacaa ataagttggt attatatgta
     8881 ccaacatata gagaagataa agcagataat agggctattg ataaagctta ttttgaaaaa
     8941 tgtttaccag gatatacact gattaataaa ttacatccat caattgaaga ttcagacatt
     9001 gatgacgtat cttcaatcga cacgtctaca ttaatgctaa tgtcagatat aattattagc
     9061 gactatagtt cgctgccaat agaagctagc ttgttagata ttccaactat attttatgtg
     9121 tatgatgaag gaacatatga taaagtgaga ggcctgaatc aattttacaa agcaataccg
     9181 gatagctaca aagtgtatac tgaagaagat ttaataatga cgatacaaga aaaagaacat
     9241 ctattaagtc cgttatttaa agattggcat aagtataata ctgataaaag tttacatcag
     9301 ctcacagaat atatagataa gatggtgaca aaatgaggct tacgataatc atacctacat
     9361 gtaataatga ggcaacaatt cgacaattgt taatatctat tgagagtaaa gaacactata
     9421 gaatcctttg tattgatggt ggttctactg atcaaacaat tcctatgatt gaacggttac
     9481 aaagagaact caagcatatt tcattaatac aattacaaaa tgcttcgata gctacgtgta
     9541 ttaataaagg tttgatggat atcaaaatga cagatccaca tgatagtgac gcatttatgg
     9601 tcataaatcc aacatcaatc gtattgccag gtaaattaga taggttaact gcagctttca
     9661 aaaataatga taatattgat atggtaatag ggcagcgagc ttacaattac catggtgaat
     9721 ggaaattgaa aagtgctgat gagtttatta aagataatcg aatcgttaca ttaacggaac
     9781 aaccagattt gttatcaatg atgtcttttg acggaaagtt attcagtgct aaatttgctg
     9841 aattacagtg tgacgaaact ttagctaaca catacaatca cgcaatactt gtcaaggcga
     9901 tgcaaaaagc tacggatata catttagttt cacagatgat tgtcggagat aacgatatag
     9961 atacacatgc tacaagtaac gatgaagatt ttaatagata tatcatagaa attatgaaaa
    10021 taagacaacg agtcatggaa atgttactat tacctgaaca aaggctatta tatagtgata
    10081 tggttgatcg tattttattc aataattcat taaaatatta tatgaatgaa cacccagcag
    10141 taacgcacac gacaattcaa ctcgtaaaag actatattat gtctatgcag cattctgatt
    10201 atgtatcgca aaacatgttt gacattataa atacagttga atttattggt gagaattggg
    10261 atagagaaat atacgaattg tggcgacaaa cattaattca agtgggcatt aataggccga
    10321 cttataaaaa attcttgata caacttaaag ggagaaagtt tgcacatcga acaaaatcaa
    10381 tgttaaaacg ataacgtgta tattgatgac cataaactgc aatcctatga tgtgacaata
    10441 tgaggaggat aacttaatga aacgtgtaat aacatatggc acatatgact tacttcacta
    10501 tggtcatatc gaattgcttc gtcgtgcaag agagatgggc gattatttaa tagtagcatt
    10561 atcaacagat gaatttaatc aaattaaaca taaaaaatct tattatgatt atgaacaacg
    10621 aaaaatgatg cttgaatcaa tacgctatgt cgatttagtc attccagaaa agggctgggg
    10681 acaaaaagaa gacgatgtcg aaaaatttga tgtagatgtt tttgttatgg gacatgactg
    10741 ggaaggtgaa ttcgacttct taaaggataa atgtgaagtc atttatttaa aacgtacaga
    10801 aggcatttcg acgactaaaa tcaaacaaga attatatggt aaagatgcta aataaattat
    10861 atagaactat cgatactaaa cgataaatta acttaggtta ttataaaata aatataaaac
    10921 ggacaagttt cgcagcttta taatgtgcaa cttgtccgtt tttagtatgt tttattttct
    10981 ttttctaaat aaacgattga ttatcatatg aacaataagt gctaatccag cgacaaggca
    11041 tgcaccacca atgatagtga ataatggatg ttcttcccac atacttttag caacagtatt
    11101 tgccttttga ataattggct gatgaacttc tacagttgga ggtccataat ctttattaat
    11161 aaattctctt ggatagtccg cgtgtacttt accatcttcg actacaagtt tataatcttt
    11221 tttactaaaa tcacttggta aaacatcgta aagatcattt tcaacataat atttcttacc
    11281 atttatcctt tgctcacctt tagacaatat ttttacatat ttatactgat caaatgagcg
    11341 ttccattaat gcattcccca tcatattacg ttgcttctcg ccaccaaggt ttttatagtc
    11401 tcctgcaccc atgataactt gattaattct aaatttacct cgtttggtag taatcgtatg
    11461 gttgtaattt gctgtatcac ttgatccagt ttttaaacca tctgtaccag gcaaactcat
    11521 ttttgcacct tccaatgaaa agttgaatgt gtaatacgta actgcatgcg ttgttggtgc
    11581 taactgcttt gtaaagtcta atattttagg tgtctcttta atcacgtgta aatctaaaat
    11641 ggcatagtct ctagcagtcg ttacagtacg ttcttggtct ttatactttg ttggtgcaaa
    11701 tgaacgtaat cttgaatttt cagcacccgt tggattgacg aaatgtgtat ttttcattcc
    11761 gatagcttta gctttgttat tcattaaatc aacgaaatcg ctggtgtttt ttgatacctt
    11821 cttagctaaa attaatgccg cggcattact agaattagat actgtaattt gtaataggtc
    11881 tgcgattgtc catacttgtc caggatatag tttcgtatta ctcaactcag gtagtgtaga
    11941 cataatatat tctttgttcg tcattgtgac tgtgtcatca agtgaaagct gccccttatt
    12001 tacagcttcc aatgttaagt acattgtcat taatttagtc atagacgctg gattccactt
    12061 agtatcgata ttgtattgat acagtaattg tccagtttga cttacattaa cagcactcgt
    12121 cggttcgtat gcagccgaca aacctgcata accatattga tttgctgctt gtacaggggt
    12181 tacgtcactg ttagtagctt gtgcatatgg tgtcataata cttaatgtta aacataaaat
    12241 gatgataata gatattaaat ttttcataaa gcgttaatct tcccttttcc aattcttaaa
    12301 tattccctaa aagcaatggt tattcctact tacggaaatc attgctaatt cacttcacct
    12361 taattaaatt gttgaaaata aagttttctg cagttaattt gaaaaataat gcaaatatat
    12421 tacgtgtgta gctaaaggtg ttataatgtt tgtacgaaga gcaaacttac tcaaaagcga
    12481 ttaattttca tgttttaata taaagacttt gagaagttat tacaaaaaat gcaatagaaa
    12541 tattctatca tataaatgtt atgagcggta ttttggggca acactttatt tgatttttaa
    12601 agttttgttg ggagaaagta tatgatagaa atgcatgtat ctatctaaat gaattaacta
    12661 taaatttcaa acagaagagg taaaactatg aaacgagaaa atccattgtt tttcttattt
    12721 aaaaaactat catggccagt gggtcttatc gttgcagcta tcactatttc atcactaggg
    12781 agcttaagtg gactattagt gccactgttt actggacgaa ttgtagataa attttccgtg
    12841 agccatatca attggaatct aatcgcatta tttggtggta tctttgtcat caatgcttta
    12901 ttaagcggat taggtttata tttattaagt aaaattggtg aaaagattat ttatgcgata
    12961 cgctcagttt tatgggagca tatcatacaa ttaaaaatgc cattctttga caaaaatgaa
    13021 agtggtcaat taatgagtcg attaactgac gatacgaaag tgataaatga atttatttca
    13081 caaaagctac ctaacttatt accatcaatc gttacattag ttgggtcact aatcatgtta
    13141 tttattttag attggaaaat gacattatta acatttataa cgataccgat attcgtttta
    13201 attatgattc ctctaggtcg tattatgcaa aagatatcga caagtacaca atctgaaatt
    13261 gcaaacttca gtggtttgtt agggcgtgtc ctaactgaaa tgcgtcttgt taaaatatca
    13321 aatacagagc gtcttgaatt agataatgca cataaaaatt tgaatgaaat atataaatta
    13381 ggtttaaaac aggctaaaat tgcggcagtt gtacaaccaa tttcaggtat agttatgttg
    13441 ctaacaattg caattatttt aggttttggt gcattagaaa ttgcgactgg tgcaatcact
    13501 gcaggtacat taattgcaat gatattttat gttattcagt tatctatgcc tttaatcaat
    13561 ctttccacgt tagttacaga ttataaaaag gcagtcggtg caagtagtag aatatacgaa
    13621 atcatgcaag aacctattga accgacagaa gctcttgaag attctgaaaa tgtattaatt
    13681 gatgacggtg tattgtcatt tgaacatgta gactttaaat atgatgtgaa gaaaatatta
    13741 gatgatgtgt cgttccaaat cccacaaggt caagtgagtg cttttgtagg tccttctggg
    13801 tctggtaaaa gtacgatatt taatctgata gaacgtatgt atgaaattga gtcaggtgat
    13861 attaaatatg gccttgaaag tgtctatgat atcccgttat ctaagtggcg acgcaaaatt
    13921 ggatatgtta tgcaatcaaa ttcgatgatg agtggaacaa ttagagacaa tattttatac
    13981 ggaattaatc gtcatgtttc agatgaagaa cttattaatt atgctaaatt agcgaactgt
    14041 catgatttta tcatgcaatt tgatgaagga tatgacacgc ttgtaggtga acgaggattg
    14101 aaactgtctg gcggacaacg tcaacgtatt gatattgcta gaagttttgt taaaaatcct
    14161 gatattttgt tacttgatga agcaacagct aatctcgata gtgaaagtga attgaaaatt
    14221 caagaagctt tagaaacatt gatggaaggt agaacaacga ttgtcattgc gcatcgtttg
    14281 tctacaatta aaaaagccgg tcaaattata ttcttagaca aaggacaggt aacaggtaaa
    14341 ggtacgcatt cagaactgat ggcatcacat gcgaagtata aaaactttgt agtgtctcaa
    14401 aaattaacag attaatttta tatataagta agcttggagc aaatacacat ataccatcga
    14461 ggaaattaaa gtgtggcaca ttgatggata tagatgttaa taaattgctt caagcttttg
    14521 tctattttaa atcatttgag aagttacgac ataataattc ttaaattaat aaaatcgata
    14581 ttttaagaaa aaaatgctca tggtataata caagttataa gcaaacatac atatattaaa
    14641 tactgtagcc acgagtcata attcttcata ttttacatag caatttaact gattttagag
    14701 tccacggtac agaagtttga tatttcaatg tttctaaatt tttaaaaaat taaatcatag
    14761 gtgggtgcta aatgttttta ttaatcaaca ttattggtct aattgtattt cttggtattg
    14821 cggtattatt ttcaagagat cgcaaaaata tccaatggca atcaattggg atcttagttg
    14881 ttttaaacct gtttttagca tggttcttta tttattttga ttggggtcaa aaagcagtaa
    14941 gaggagcagc caatggtatc gcttgggtag ttcagtcagc gcatgctggt acaggttttg
    15001 catttgcaag tttgacaaat gttaaaatga tggatatggc tgttgcagcc ttattcccaa
    15061 tattattaat agtgccatta tttgatatct taatgtactt taatatttta ccgaaaatta
    15121 ttggaggtat tggttggtta ctagctaaag taacaagaca acctaaattc gagtcattct
    15181 ttgggataga aatgatgttc ttaggaaata ctgaagcatt agccgtatca agtgagcaac
    15241 taaaacgtat gaatgaaatg cgtgtattaa caatcgcaat gatgtcaatg agctctgtat
    15301 ccggagctat tgtaggtgcg tatgtacaaa tggtaccagg agaactggta ctaacggcaa
    15361 ttccactaaa tatcgttaac gcgattattg tgtcatgctt gttgaatcca gtaagtgttg
    15421 aagagaaaga agatattatt tacagtctta aaaacaatga agttgaacgt caaccattct
    15481 tctcattcct tggagattct gtattagcag caggtaaatt agtattaatc atcatcgcat
    15541 ttgttattag ttttgtagcg ttagctgatc tatttgatcg ttttatcaat ttgattacag
    15601 gattgatagc aggatggata ggcataaaag gtagtttcgg tttaaaccaa attttaggtg
    15661 tgtttatgta tccatttgcg ctattactcg gtttacctta tgatgaagcg tggttggtag
    15721 cacaacaaat ggctaagaaa attgttacaa atgaatttgt tgttatgggt gaaatttcta
    15781 aagatattgc atcttataca ccacaccatc gtgcggttat tacaacattc ttaatttcat
    15841 ttgcaaactt ctcaacgatt ggtatgatta tcggtacatt gaaaggcatt gttgataaaa
    15901 agacatcaga ctttgtatct aaatatgtac ctatgatgct attatcaggt atcctagttt
    15961 cattattaac agcagctttc gttggtttat ttgcatggta atatgtcgaa gagtgactat
    16021 gataatacat tttaactaat aaatatgtcc aggcatgtcg tctattgata taggtgagat
    16081 gcttggactt ttttattatt gatataaagg tatttaaata tttttaaagt taccgaaatt
    16141 gaagcattat aaaaaccaag tgcacatggt aatacacttg gcttttatgg gaaatgaata
    16201 ttattgtaca tatgacagta aggactaggt acagtcatag tacttcgagc aaaatttgtt
    16261 ttgttattat aaacaacaca aaggagataa cttctctatt gaagaagtta aaaacattat
    16321 agcagacaat gaaatgaaag taaatta
//
LOCUS       DAHHSC010000042        15952 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.042, whole genome shotgun sequence.
ACCESSION   DAHHSC010000042 DAHHSC010000000
VERSION     DAHHSC010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 15952)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15952)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15952
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.042"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            555..1184
                     /locus_tag="NBI57_001032"
     CDS             555..1184
                     /locus_tag="NBI57_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4887 domain-containing protein"
                     /protein_id="HCD1821995.1"
                     /translation="MSNQNYDYNKNEDGSKKKMSTTAKVVSIATVLLLLGGLVFAIFA
                     YVDHSNKAKERMLNEQKQEQKEKRQKENAEKERKKKQQEEKEQNELDSQANQYQQLPQ
                     QNQYQYVPPQQQAPTKQRPAKEENDDKASKDESKDKDDNASQDKSDDNQKKTDDNKQP
                     AQPKPQPQQPTPKPNNNQQNNQSNQQAKPQAPQQNSQSTTNKQNNANDK"
     gene            1997..3010
                     /locus_tag="NBI57_001033"
     CDS             1997..3010
                     /locus_tag="NBI57_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HCD1821996.1"
                     /translation="MKDLLQAQQKLIPDLIDKMYKRFSILTTISKNQPVGRRSLSEHM
                     DMTERVLRSETDMLKKQDLIKVKPTGMEITAEGEQLISQLKGYFDIYADDNRLSEGIK
                     NKFQIKEVHVVPGDADNSQSVKTELGRQAGQLLEGILQEDAIVAVTGGSTMACVSEAI
                     HLLPYNVFFVPARGGLGENVVFQANTIAASMAQQAGGYYTTMYVPDNVSETTYNTLLL
                     EPSVINTLDKIKQANVILHGIGDALKMAHRRQSPEKVIEQLQHHQAVGEAFGYYFDTQ
                     GQIVHKVKTIGLQLEDLESKDFIFAVAGGKSKGEAIKAYLTIAPKNTVLITDEAAAKI
                     ILE"
     gene            3063..4073
                     /gene="gap"
                     /locus_tag="NBI57_001034"
     CDS             3063..4073
                     /gene="gap"
                     /locus_tag="NBI57_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HCD1821997.1"
                     /translation="MAVKVAINGFGRIGRLAFRRIQEVEGLEVVAVNDLTDDDMLAHL
                     LKYDTMQGRFTGEVEVVDGGFRVNGKEVKSFSEPDASKLPWKDLNIDVVLECTGFYTD
                     KDKAQAHIEAGAKKVLISAPATGDLKTIVFNTNHQELDGSETVVSGASCTTNSLAPVA
                     KVLNDDFGLVEGLMTTIHAYTGDQNTQDAPHRKGDKRRARAAAENIIPNSTGAAKAIG
                     KVIPEIDGKLDGGAQRVPVATGSLTELTVVLEKQDVTVEQVNEAMKNASNESFGYTED
                     EIVSSDVVGMTYGSLFDATQTRVMSVGDRQLVKVAAWYDNEMSYTAQLVRTLAYLAEL
                     SK"
     gene            4212..5402
                     /locus_tag="NBI57_001035"
     CDS             4212..5402
                     /locus_tag="NBI57_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HCD1821998.1"
                     /translation="MAKKIVSDLDLKGKTVLVRADFNVPLKDGEITNDNRIVQALPTI
                     QYIIEQGGKIVLFSHLGKVKEESDKAKLTLRPVAEDLSKKLDKEVVFVPETRGEKLEA
                     AIKDLKEGDVLLVENTRYEDLDGKKESKNDPELGKYWASLGDVFVNDAFGTAHREHAS
                     NVGISTHLETAAGFLMDKEIKFIGGVVNDPHKPVVAILGGAKVSDKINVIKNLVNIAD
                     KIIIGGGMAYTFLKAQGKEIGISLLEEDKIDFAKDLLEKHGDKIVLPVDTKVAKEFSN
                     DAKITVVPSDSIPADQEGMDIGPNTVKLFADELEGAHTVVWNGPMGVFEFSNFAQGTI
                     GVCKAIANLKDAITIIGGGDSAAAAISLGFENDFTHISTGGGASLEYLEGKELPGIKA
                     INNK"
     gene            5524..6285
                     /locus_tag="NBI57_001036"
     CDS             5524..6285
                     /locus_tag="NBI57_001036"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HCD1821999.1"
                     /translation="MRTPIIAGNWKMNKTVQEAKDFVNALPTLPDSKEVESVICAPAI
                     QLDALTTAVKEGKAQGLEIGAQNTYFEDNGAFTGETSPVALADLGVKYVVIGHSERRE
                     LFHETDEEINKKAHAIFKHGMTPIICVGETDEERESGKANDVVGEQVKKAVAGLSEDQ
                     LKSVVIAYEPIWAIGTGKSSTSEDANEMCAFVRQTIADLSSKEVSEATRIQYGGSVKP
                     NNIKEYMAQTDIDGALVGGASLKVEDFVQLLEGAK"
     gene            6288..7805
                     /locus_tag="NBI57_001037"
     CDS             6288..7805
                     /locus_tag="NBI57_001037"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HCD1822000.1"
                     /translation="MAKKPTALIILDGFANRESEHGNAVKLANKPNFDRYYNKYPTTQ
                     IEASGLDVGLPEGQMGNSEVGHMNIGAGRIVYQSLTRINKSIEDGDFFENDVLNNAIA
                     HVNSHDSALHIFGLLSDGGVHSHYKHLFALLELAKKQGVEKVYVHAFLDGRDVDQKSA
                     LKYIEETEAKFNELGIGQFASVSGRYYAMDRDKRWEREEKAYNAIRNFDAPTYATAKE
                     GVEASYNEGLTDEFVVPFIVENQNDGVNDGDAVIFYNFRPDRAAQLSEIFANRAFEGF
                     KVEQVKDLFYATFTKYNDNIDAAIVFEKVDLNNTIGEIAQNNNLTQLRIAETEKYPHV
                     TYFMSGGRNEEFKGERRRLIDSPKVATYDLKPEMSAYEVKDALLEELNKGDLDLIILN
                     FANPDMVGHSGMLEPTIKAIEAVDECLGEVVDKILDMDGYAIITADHGNSDQVLTDDD
                     QPMTTHTTNPVPVIVTKEGVTLRETGRLGDLAPTLLDLLNVEQPEDMTGESLIKH"
     gene            7935..9239
                     /gene="eno"
                     /locus_tag="NBI57_001038"
     CDS             7935..9239
                     /gene="eno"
                     /locus_tag="NBI57_001038"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017466665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HCD1822001.1"
                     /translation="MPIITDVYAREVLDSRGNPTVEVEVLTESGAFGRALVPSGASTG
                     EHEAVELRDGDKSRYLGKGVTKAVENVNEIIAPEIIEGEFSVLDQVSIDKMMIALDGT
                     PNKGKLGANAILGVSIAVARAAADLLGQPLYKYLGGFNGKQLPVPMMNIVNGGSHSDA
                     PIAFQEFMILPVGATTFKESLRWGTEIFHNLKSILSKRGLETAVGDEGGFAPKFEGTE
                     DAVETIIQAIEAAGYKPGEEVFLGFDCASSEFYENGVYDYSKFEGEHGAKRTAAEQVD
                     YLEQLVDKYPIITIEDGMDENDWDGWKQLTERIGDRVQLVGDDLFVTNTEILAKGIEN
                     GIGNSILIKVNQIGTLTETFDAIEMAQKAGYTAVVSHRSGETEDTTIADIAVATNAGQ
                     IKTGSLSRTDRIAKYNQLLRIEDELFETAKYDGIKSFYNLDK"
     gene            9578..10036
                     /locus_tag="NBI57_001039"
     CDS             9578..10036
                     /locus_tag="NBI57_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1822002.1"
                     /translation="MADRTNKEIKTGRFIATASIVFSILLIIHYFVSLDNATAKALLN
                     LTNQNTSDKAIDYILNSFRFTGIMYILAYLAGFITFWNRHTYVWWFMFAVYVSNSLFT
                     LINLSITIQAIKAAHGAYLTLPILIVIIGSVALAIYMLVVSIKRKSTFNR"
     gene            10103..10336
                     /gene="secG"
                     /locus_tag="NBI57_001040"
     CDS             10103..10336
                     /gene="secG"
                     /locus_tag="NBI57_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HCD1822003.1"
                     /translation="MHTFLIVLLIIDCIALITVVLLQEGKSSGLSGAISGGAEQLFGK
                     QKQRGVDLFLNRLTIILSILFFVLMICISYLGM"
     gene            10452..11192
                     /locus_tag="NBI57_001041"
     CDS             10452..11192
                     /locus_tag="NBI57_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylesterase"
                     /protein_id="HCD1822004.1"
                     /translation="MQIKLPKPFFFEEGKRAVLLLHGFTGNSSDVRQLGRFLQKKGYT
                     SYAPQYEGHAAPPEEILKSSPFVWFKDALDGYDYLVEQGYDEIVVAGLSLGGDFALKL
                     SLNRDVKGIVTMCAPMGGKTEGAIYEGFLEYARNFKKYEGKNQETIDNEMDHFKPTET
                     LKELSEALDTIKDQVDEVFDPILVIQVENDNMIDPQSANYIYDHVDSDDKDIKWYSQS
                     GHVITIDKEKEQVFEDIYQFLESLDWSE"
     gene            11226..13598
                     /gene="rnr"
                     /locus_tag="NBI57_001042"
     CDS             11226..13598
                     /gene="rnr"
                     /locus_tag="NBI57_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HCD1822005.1"
                     /translation="MNLKQSIEEIINQPEYEPMSVSDFQDALGLSSADSFRDLIKVLV
                     ELEQSGLIERTKTDRYQKKHSSKGHSKLIKGTLSQNKKGFAFLRPEDEDMEDIFIPPT
                     KINRALDGDTVIVEIHQSKGEHKGKIEGEVKSIEKHSVTQVVGTYSEARHFGFVIPDD
                     KRIMQDIFIPKGQSLGAVDGHKVLVQITKYADGSDNPEGHISAILGHKNDPGVDILSI
                     IYQHGIEIEFPDEVLQEAEAVPDHIENTEIKGRHDLRDELTITIDGADAKDLDDAISV
                     KKLANGNTQLTVSIADVSYYVTEDSALDKEAYDRATSVYLVDRVIPMIPHRLSNGICS
                     LNPHVDRLTLSCRMEIDASGRVVKHEIFDSVIHSDYRMTYDAVNQIITEKDPNIREQY
                     KEITPMLDLAQDLSNRLIQMRKRRGEIDFDISEAKVLVNEVGIPTDVQLRQRGEGERL
                     IESFMLIANETVAEHFSKLNVPFIYRVHEQPKSDRLRQFFDFITNFGIMIKGTGEDIH
                     PTTLQKVQEEVEGRPEQMVISTMMLRSMQQAHYDDVNLGHFGLSAEYYTHFTSPIRRY
                     PDLTVHRLIRKYLIEKSMDNKEVKRWEDKLPELAEHTSKRERRAIEAERDTDELKKAE
                     YMIQHIGDEFEGIVSSVANFGMFIELPNTIEGMVHIANMTDDYYRFEERQMALIGERQ
                     AKVFRIGDTVKVKVTHVDVDERLIDFQIVGMPLPKNDRSQRPARGKTIQAKTRGKSLD
                     KSKSDDKGRKKKGKQRKGKNQRNNDKSGNSKHKPFYKDKSVKKKARRKKK"
     gene            13620..14084
                     /gene="smpB"
                     /locus_tag="NBI57_001043"
     CDS             13620..14084
                     /gene="smpB"
                     /locus_tag="NBI57_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HCD1822006.1"
                     /translation="MAKKKSPGTLAENRKARHDYNIEDTIEAGIVLQGTEIKSIRRGS
                     ANLKDSYAQVKNGEMYLNNMHIAPYEEGNRFNHDPLRSRKLLLHKREIFKLGEQTREI
                     GYSIVPLKLYLKHGHCKVLLGVARGKKKYDKRQALKEKAVKRDVARDMKARY"
     gene            14175..14536
                     /gene="ssrA"
                     /locus_tag="NBI57_001044"
     tmRNA           14175..14536
                     /gene="ssrA"
                     /locus_tag="NBI57_001044"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(14647..15753)
                     /locus_tag="NBI57_001045"
     CDS             complement(14647..15753)
                     /locus_tag="NBI57_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015728767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCD1822007.1"
                     /translation="MSYNLNLSHNIYKDAKLGTYYFRITYYDKSNTRKYITRKGFKQR
                     KDAVKKCNEMMDELEGVGHLNRLPFDKLVEEYIDWYSARRKTSSVKALKTHTNNHLLP
                     YFKSMDVFKMTTQDVMKFQNKKLKEGHSGDYLKKMHVYLVSLLNHAMKFHELKQNVAS
                     LVGNFEIESQKRLNYWTLEQFNQFYEALATQQQKLFFKLLFYSGARKGEIRALTWRDI
                     NFDDDFIHINKTDYHGEVTAPKTKSAIRDIYLPTHMMYDIKDYLIWYKENNIYKDDYV
                     LFGTFYKAYSESTIDRWFTNALKVLDEQLPNGQNFPRIVIHELRHSHASMLVNLGASV
                     MIIAQRLGHSDTTEVYNRYGHLYPSTQKEIVKYL"
     gene            complement(15759..>15952)
                     /locus_tag="NBI57_001046"
     CDS             complement(15759..>15952)
                     /locus_tag="NBI57_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000390815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HCD1822008.1"
                     /translation="AYQRTINAQELVVDYYEDSKAKEFLKDESIDEFLTTISNKYFDL
                     LEYIDDYRVKHDLEKISEE"
BASE COUNT         5562 a         2440 c         3100 g         4850 t
ORIGIN      
        1 gtaccttcga taacgaataa acatctctta aaagtatgtg taaaacgctg catgatacaa
       61 acgaaggtaa aaatttgact ccctttagta gtggacccgt acgttaatcg tgcgggtcgt
      121 ttttatttat ttcttattcc cattatacat caatttaaag cattaatttt ttaaacaaat
      181 ttaagaatac atagtaatat aacaatctaa acataaaaac ttttaacaca acacttaaac
      241 caatgcttta attttcaata cgtagctata atttgttgta aaatcaaaaa ggttaaaatg
      301 ttaattttca aaaaaaggct caaaatatgt ttgatttagt tattaaatgt taagatatat
      361 aagactacta tttctttgta aaaatgaatc cgatttacga gtgagtaata gtgaaggcag
      421 ttttaagttg aagaaggcaa aaagagtaaa tgtttattaa tatttgtaga aactaggtaa
      481 gcaaattagt tgtgaaaatg ttaatggttg cgtgataatt tctatattta aattagtttg
      541 aagtgaggga gagtatgtcg aatcaaaatt acgactacaa taaaaatgaa gatggaagta
      601 agaagaaaat gagtacaaca gcgaaagtag ttagcattgc gacggtattg ctattactcg
      661 gaggattagt atttgcaatt tttgcatatg tagatcattc gaataaagct aaagaacgta
      721 tgttgaacga acaaaagcag gaacaaaaag aaaagcgtca aaaagaaaat gcagaaaaag
      781 agagaaagaa aaagcaacaa gaggaaaaag agcagaatga gctagattca caagcaaacc
      841 aatatcagca attgccacag cagaatcaat atcaatatgt gccacctcag caacaagcac
      901 ctacaaagca acgtcctgct aaagaagaga atgatgataa agcatcaaag gatgagtcga
      961 aagataagga tgacaatgca tctcaagata aatcagatga taatcagaag aaaactgatg
     1021 ataataaaca accagctcag cctaaaccac agccgcaaca accaacacca aagccaaata
     1081 ataatcaaca aaataatcaa tcaaatcagc aagcaaaacc acaagcacca caacaaaata
     1141 gccaatcaac aacaaataaa caaaataatg ctaatgataa gtagtattta gtcaaacaaa
     1201 aatgaaccag tatgacagac aacacaatta attaggttgt ctcgaatatt ggttcttatt
     1261 tttataattg ttaattaggg gagagatgat acttaaatag ttagttgttt attttacgga
     1321 tagtgaaatt tattttgagt gaggtgggac agaaatgata ttttcgcaaa atttatttcg
     1381 tcgtctgacc ccaacttcca cattattgta agctgacttt ccgtcagctt ctgtgttggg
     1441 gccccgccaa cttgcacatt attgtaagct gactttccgt cagcttctgt gttggggccc
     1501 cgccaacttg cacattattg taagctgact ttccgccagc ttctgtgttg gggccccacc
     1561 ccaacttgca ttgcctgtag aaattggaaa tccaatttct ctatgttggg gcccctgact
     1621 ttaattggaa aaagcttgtt acaagtgcat tttcgttcgg tcaactacta ctaatgtgac
     1681 ttttcggatt ctagagcatt gttttatgtc ttagtctcaa ataataagca aatgattagc
     1741 gagtcagtat aagggtatac atttgactac agcgaataaa ataaaacgtt ttgttgaata
     1801 acaaatcgaa aatgtattgc aagcgcttta tcaaattatt tagaaaattt aagttttatg
     1861 cttgcaattt ttgaaataga atagtactat tgcaagtgta aagaggttaa tttttgtccc
     1921 acgcgggact taaaaaggca accactggtt gtgacatatc cttatttaca tttataaata
     1981 taaggaggag gtagtagtga aagacttatt gcaagcacag caaaagctta taccggatct
     2041 catagataaa atgtataaac gtttttctat tcttactact atctcaaaaa atcagcctgt
     2101 cggacgtcga agtttaagcg aacatatgga tatgactgaa cgtgtactgc gttctgaaac
     2161 agatatgctt aagaaacaag atttgataaa agttaagcct accggaatgg aaattacagc
     2221 tgaaggtgag caactgattt cgcaattgaa aggttacttt gatatctatg cagatgataa
     2281 tcgtctgtca gaaggtatta agaataaatt tcaaattaag gaagttcatg ttgttcctgg
     2341 tgatgctgat aatagtcaat ctgttaaaac agaattaggt agacaagcag gtcaattact
     2401 tgaaggcata ttacaagaag acgcgatagt tgctgtaact ggcggatcca cgatggcatg
     2461 tgttagtgaa gcaattcatt tattaccata taatgtattc ttcgtaccag ccagaggtgg
     2521 actaggcgaa aatgttgtct ttcaggcaaa cacaattgca gccagtatgg cacaacaagc
     2581 tggcggttat tatacgacga tgtatgtacc tgataatgtc agtgaaacaa catataacac
     2641 attgttgtta gagccatcag tcataaacac tttagacaaa attaaacaag caaacgttat
     2701 attacacggc attggtgatg cgctgaagat ggcgcatcga cgtcaatcac ctgaaaaggt
     2761 cattgaacaa cttcaacatc atcaagctgt cggagaggca tttggttatt attttgatac
     2821 acaaggtcaa attgtccata aggttaaaac aattggactt caattagaag accttgaatc
     2881 aaaagacttt atttttgcag ttgcaggagg caaatcgaaa ggtgaagcaa ttaaagcata
     2941 cttgacgatt gcacccaaga atacagtgtt aatcactgat gaagccgcag caaagataat
     3001 acttgaataa gagataaaaa gtttaatact ttttaaatat cattttaaag gaggccatta
     3061 taatggcagt aaaagtagca attaatggtt ttggtagaat tggtcgttta gcattcagaa
     3121 gaattcaaga agtagaaggt cttgaagttg tagcagtaaa cgacttaaca gatgacgaca
     3181 tgttagcgca tttattaaaa tatgacacta tgcaaggtcg tttcacaggt gaagtagagg
     3241 tagttgatgg tggtttccgc gtaaatggta aagaagttaa atcattcagt gaaccagatg
     3301 caagcaaatt accttggaaa gacttaaata tcgatgtagt gttagaatgt actggtttct
     3361 acactgataa agataaagca caagctcata ttgaagcagg cgctaaaaaa gtattaatct
     3421 cagcaccagc tactggtgac ttaaaaacaa tcgtattcaa cactaaccac caagagttag
     3481 acggttctga aacagttgtt tcaggtgctt catgtactac aaactcatta gcaccagttg
     3541 ctaaagtttt aaacgatgac tttggtttag ttgaaggttt aatgactaca attcacgctt
     3601 acacaggtga tcaaaataca caagacgcac ctcacagaaa aggtgacaaa cgtcgtgctc
     3661 gtgcagcggc agaaaacatc atccctaact caacaggtgc tgctaaagct atcggtaaag
     3721 ttattcctga aatcgatggt aaattagatg gtggtgcaca acgtgttcct gtagctacag
     3781 gttcattaac tgaattaaca gtagtattag aaaaacaaga cgtaacagtt gaacaagtta
     3841 acgaagctat gaaaaatgct tcaaacgaat cattcggtta cactgaagac gaaatcgttt
     3901 cttcagacgt tgtaggtatg acttacggtt cattattcga cgctacacaa actcgtgtaa
     3961 tgtcagttgg cgaccgtcaa ttagttaaag ttgcagcttg gtatgataac gaaatgtcat
     4021 atactgcaca attagttcgt acattagcat acttagctga actttctaaa taattttagt
     4081 atagttttta ttcaaatacg ctagtgctca gaactattta gcattaatta aagcttatga
     4141 gtaagcgggg agcacaaacg cttctccgct tatttttata taaaatttcc taattacaag
     4201 gaggaaacac catggctaaa aaaattgttt ctgatttaga tcttaaaggt aaaacagtcc
     4261 tagtacgtgc tgattttaac gtacctttaa aagacggtga aattactaat gacaaccgta
     4321 tcgttcaagc tttacctaca attcaataca tcatcgaaca aggtggtaaa atcgtactat
     4381 tttcacattt aggtaaagtg aaagaagaaa gtgataaagc aaaattaact ttacgtccag
     4441 ttgctgaaga cttatctaag aaattagata aagaagttgt tttcgtacca gaaacacgcg
     4501 gcgaaaaact tgaagctgct attaaagacc ttaaagaagg cgacgtatta ttagttgaaa
     4561 atacacgtta tgaagattta gacggtaaaa aagaatctaa aaatgatcca gaattaggta
     4621 aatactgggc atctttaggt gatgtgtttg taaatgatgc ttttggtact gcgcatcgtg
     4681 agcatgcatc taatgttggt atttctacac atttagaaac tgcagctgga ttcttaatgg
     4741 ataaagaaat taagtttatt ggtggcgtag ttaacgatcc acataaacca gttgttgcta
     4801 ttttaggtgg agcaaaagta tctgacaaaa ttaatgtcat caaaaactta gttaacatag
     4861 ctgataaaat tatcatcggc ggaggtatgg cttatacttt cttaaaagcg caaggtaaag
     4921 aaattggtat ttcattatta gaagaagata aaatcgactt cgcaaaagat ttattagaaa
     4981 aacatggtga taaaattgta ttaccagtag acactaaagt tgctaaagaa ttttctaatg
     5041 atgccaaaat cactgtagta ccatctgatt caattccagc agaccaagaa ggtatggata
     5101 ttggaccaaa cactgtaaaa ttatttgcag atgaattaga aggtgcgcac actgttgtat
     5161 ggaatggacc tatgggtgta ttcgagttca gtaactttgc acaaggtaca attggtgtat
     5221 gtaaagcaat tgcaaacctt aaagatgcaa ttacgattat cggtggcggt gattcagctg
     5281 cagcagcaat ctctttaggt tttgaaaatg acttcactca tatttcaact ggtggcggcg
     5341 cgtcattaga gtacctagaa ggtaaagaat tgcctggtat caaagcaatc aataataaat
     5401 aataaagtga tagtttaaag tgatgtggca tgtttgttta acattgttac gggaaaacag
     5461 tcacaagatg acatcgtgtt tcatcacttt tcaaaaatat ttacaaaaca aggagtgtct
     5521 ttaatgagaa caccaattat agctggtaac tggaaaatga acaaaacagt acaagaagca
     5581 aaagacttcg tcaatgcatt accaacatta ccagattcaa aagaagtaga atcagtaatt
     5641 tgtgcaccag caattcaatt agatgcatta actactgcag ttaaagaagg aaaagcacaa
     5701 ggtttagaaa tcggtgctca aaatacgtat ttcgaagata atggtgcgtt cacaggtgaa
     5761 acgtctccag ttgcattagc agatttaggc gttaaatacg ttgttatcgg tcattctgaa
     5821 cgtcgtgaat tattccacga aacagatgaa gaaattaaca aaaaagcgca cgctattttc
     5881 aaacatggaa tgactccaat tatttgtgtt ggtgaaacag acgaagagcg tgaaagtggt
     5941 aaagctaacg atgttgtagg tgagcaagtt aagaaagctg ttgcaggttt atctgaagat
     6001 caacttaaat cagttgtaat tgcttatgag ccaatctggg caatcggaac tggtaaatca
     6061 tcaacatctg aagatgcaaa tgaaatgtgt gcatttgtac gtcaaactat tgctgactta
     6121 tcaagcaaag aagtatcaga agcaactcgt attcaatatg gtggtagtgt taaacctaac
     6181 aacattaaag aatacatggc acaaactgat attgatgggg cattagtagg tggcgcatca
     6241 cttaaagttg aagatttcgt acaattgtta gaaggtgcaa aataatcatg gctaagaaac
     6301 caactgcgtt aattatttta gatggttttg cgaaccgcga aagcgaacat ggtaatgcgg
     6361 taaaattagc aaacaagcct aattttgatc gttattacaa caaatatcca acgactcaaa
     6421 tcgaagcgag tggcttagat gttggactac ctgaaggaca aatgggtaac tcagaagttg
     6481 gtcatatgaa tatcggtgca ggacgtatcg tttatcaaag tttaactcga atcaataaat
     6541 caattgaaga cggtgatttc tttgaaaatg atgttttaaa taatgcaatt gcacacgtga
     6601 attcacatga ttcagcgtta cacatctttg gtttattgtc tgacggtggt gtgcacagtc
     6661 attacaaaca tttatttgct ttgttagaac ttgctaaaaa acaaggtgtt gaaaaagttt
     6721 acgtacacgc atttttagat ggtcgtgacg tagatcaaaa atccgctttg aaatacatcg
     6781 aagagactga agctaaattc aatgaattag gcattggtca atttgcatct gtgtctggtc
     6841 gttattatgc aatggaccgt gacaaacgtt gggaacgtga agaaaaagct tacaatgcta
     6901 ttcgtaattt tgatgcccca acttatgcaa ctgccaaaga aggtgtagaa gcaagctata
     6961 atgagggctt aactgacgaa ttcgtagtac cattcatcgt tgagaatcaa aatgacggtg
     7021 ttaatgatgg agatgcagtg atcttctata atttccgacc tgatagagca gcacaattat
     7081 cggaaatttt tgcgaacaga gcattcgaag gctttaaagt tgaacaagtt aaagacttat
     7141 tctatgcaac attcactaag tataacgaca atatcgatgc ggctatcgtt ttcgaaaaag
     7201 ttgatttaaa taatacaatt ggtgaaattg cacaaaataa caatttaaca caattacgta
     7261 ttgcagaaac tgaaaaatat cctcacgtta cttactttat gagtggtgga cgtaacgagg
     7321 aatttaaagg tgaacgccgt cgtttaattg attcacctaa agttgcaacg tatgacttga
     7381 aaccagaaat gagtgcttat gaagttaaag atgcattatt agaagagtta aataaaggtg
     7441 acttggactt aattatttta aactttgcta accctgatat ggttggacat agtggtatgc
     7501 ttgagccgac aatcaaagca atcgaagcgg ttgatgaatg tttaggtgaa gtcgttgata
     7561 agattttaga catggacggt tatgcaatta ttactgctga ccatggtaac tctgatcaag
     7621 tattgacgga tgatgatcaa ccaatgacta cgcatacaac gaacccagta ccagtgattg
     7681 taacaaaaga aggcgttaca cttcgagaaa ctggtcgctt aggtgactta gcacctacat
     7741 tattagattt attaaatgta gaacaacctg aagacatgac aggtgaatct ttaattaaac
     7801 actaatattg taaaagatgt taagtaaacg cttaatgaca cttatttttt gaaaataata
     7861 gtaatatcat tttgttaaat gaaagaataa agctataata attatagaat aactatttaa
     7921 aggagattat aaacatgcca attattacag atgtttacgc tcgcgaagtc ttagactctc
     7981 gtggtaaccc aactgttgaa gtagaagtat taactgaaag tggcgcattt ggtcgtgcat
     8041 tagtaccatc aggtgcttca actggtgaac acgaagctgt tgaattacgt gatggagaca
     8101 aatcacgtta tttaggtaaa ggtgttacta aagcagttga aaacgttaat gaaatcatcg
     8161 caccagaaat tattgaaggt gaattttcag tattagatca agtatctatt gataaaatga
     8221 tgatcgcatt agacggtact ccaaacaaag gtaaattagg tgcaaatgct attttaggtg
     8281 tatctatcgc agtagcacgt gcagcagctg acttattagg tcaaccactt tacaaatatt
     8341 taggtggatt taatggtaag cagttaccag taccaatgat gaacatcgtt aatggtggtt
     8401 ctcactcaga tgctccaatt gcattccaag aattcatgat tttacctgta ggtgctacaa
     8461 cgttcaaaga atcattacgt tggggtactg aaattttcca caacttaaaa tcaattttaa
     8521 gcaaacgtgg tttagaaact gcagtaggtg acgaaggtgg tttcgctcct aaatttgaag
     8581 gtactgaaga tgctgttgaa acaattatcc aagcaatcga agcagctggt tacaaaccag
     8641 gtgaagaagt attcttagga tttgactgtg catcatcaga attctatgaa aatggtgtat
     8701 atgactacag taagttcgaa ggcgaacacg gtgcaaaacg tacagctgca gaacaagttg
     8761 actacttaga acaattagta gacaaatatc ctatcattac aattgaagac ggtatggacg
     8821 aaaacgactg ggatggttgg aaacaactta cagaacgtat cggtgaccgt gtacaattag
     8881 taggtgacga tttattcgta acaaacactg aaattttagc aaaaggtatt gaaaacggaa
     8941 ttggtaactc aatcttaatt aaagttaacc aaatcggtac attaactgaa acatttgatg
     9001 caatcgaaat ggctcaaaaa gctggttaca cagcagtagt ttctcaccgt tcaggtgaaa
     9061 cagaagatac aacaattgct gatattgctg ttgctacaaa cgctggtcaa attaaaactg
     9121 gttcattatc acgtactgac cgtattgcta aatacaatca attattacgt atcgaagatg
     9181 aattatttga aactgctaaa tatgacggta tcaaatcatt ctataactta gataaataat
     9241 cattttaaac attaagtact gccataattt tagttgagga ttattatgtc ggtataaatt
     9301 gaataaagtt tttgagttca cgcttaaaaa gttcacgctt gtatttataa cctgccacag
     9361 agttgagact gtggtaggtt ttttattttg aagtattatt cataacagac taataatcat
     9421 gaggtaacta ataacacata tttaacttgt attcttaaac tggtataata aatttatgtt
     9481 gaaatgaata ttgtatgaca gggtattcac ttttattaaa aggtaaaatt aaataaaggt
     9541 tttatagaac gtatttaaat atatgaggag taaacaaatg gctgatagaa cgaataaaga
     9601 aattaaaaca ggacgcttta ttgcaactgc atcaatcgta ttctcaatat tattgattat
     9661 tcattacttt gtttcgttgg ataatgcgac tgccaaagca ttacttaatt taacgaatca
     9721 aaacacttca gataaagcga ttgattacat tttaaacagc tttagattca ctggtattat
     9781 gtatattttg gcttatctag caggcttcat cactttttgg aatcgacata cttatgtgtg
     9841 gtggtttatg tttgcagttt atgtatcaaa tagtttgttt acgttgatta atttatcaat
     9901 cacaattcaa gcaataaaag ctgcacacgg tgcgtactta acattgccaa ttttaatcgt
     9961 tattataggt tcggttgcat tagcgattta tatgcttgtt gtttctatca aacgtaaaag
    10021 tacatttaat cgctagaaaa ttgattttaa caataaaaat atgatatact acttgtcgta
    10081 tataaggaac ggaggacaat ttatgcatac atttttaatc gtattattaa tcattgattg
    10141 tattgcatta ataactgttg tactactcca agaaggtaaa agcagtggac tttcaggtgc
    10201 catcagtggt ggtgctgagc agttattcgg taaacaaaaa caacgtggcg tcgatttatt
    10261 cttaaataga ttaacaatta ttttatcaat attatttttt gtacttatga tttgcataag
    10321 ttatcttggt atgtaaggtc cggcgatgta aatgtcgggc ttttttattt ataattaaga
    10381 atgtaatagt ttaacaataa gctatgtaaa atatatagcc tagttaagta tgcaaaggga
    10441 gcgttagatt tatgcagata aaattaccaa aacctttctt ttttgaagaa ggcaaacgtg
    10501 ccgtgttatt attacatgga tttacaggca attcgtctga tgtacgtcaa ttaggtcgat
    10561 ttttacaaaa aaagggatat acttcgtatg caccacaata tgaaggtcac gcggcaccac
    10621 cagaggaaat actgaaatct agtcctttcg tttggtttaa agatgcgtta gatggttatg
    10681 attatcttgt tgaacaaggt tatgatgaaa ttgttgttgc tggtctatca ttaggtgggg
    10741 attttgcttt aaaattaagc ttaaatagag atgtaaaggg tattgtaacg atgtgtgcac
    10801 caatgggtgg caaaactgaa ggtgccattt atgaaggctt tttagagtat gcacgtaatt
    10861 ttaaaaagta tgaaggtaaa aatcaagaga ctattgataa tgaaatggat cattttaaac
    10921 caactgaaac tttaaaagaa ctaagtgaag cattagatac gattaaagat caagttgatg
    10981 aggtatttga cccaatttta gtgattcaag tagaaaacga caatatgatt gatccacaat
    11041 ccgcaaatta tatatatgac catgtagatt ctgatgacaa agacatcaag tggtatagtc
    11101 aatcaggaca tgttattacg attgataaag aaaaagaaca agtatttgaa gatatttatc
    11161 aatttttaga gtcattagac tggtcagaat aaaaagagat tttaacatta gaaaggaggg
    11221 gcataatgaa tttaaagcaa tcaatagaag agattattaa tcaacctgaa tatgaaccta
    11281 tgtcagtgtc agattttcaa gatgcattag gtttaagtag tgccgactca tttagagatt
    11341 taattaaggt gctcgtagaa ttagaacaat caggattaat tgaacgtaca aaaacagaca
    11401 gatatcaaaa aaagcatagt tcaaaaggtc attcaaaatt gataaaggga acgttaagtc
    11461 aaaataaaaa aggctttgca ttcttaagac ctgaagatga ggatatggaa gatatattta
    11521 ttcccccaac aaaaattaat cgtgccttag atggagatac tgtaattgta gaaatacatc
    11581 aatctaaagg tgaacataaa ggtaaaattg aaggggaagt taagtcgatt gagaagcatt
    11641 cagtaactca agttgttggt acgtatagtg aagctagaca tttcggattt gttattccag
    11701 atgataaacg tattatgcaa gatattttca ttcctaaagg acaaagttta ggtgcagtag
    11761 atgggcataa ggtacttgtt caaattacta aatatgctga tggttcagat aatccagaag
    11821 gacatatttc tgctatttta ggacataaaa atgatcctgg cgtagatatt ttatctatta
    11881 tctatcaaca tggcatagaa attgaatttc ctgatgaagt gttacaagaa gctgaagcag
    11941 tacctgatca tattgaaaat actgaaatta aaggccgtca tgatttacgt gatgaattga
    12001 caatcacaat tgatggtgct gatgctaaag acttagatga cgcaattagt gttaaaaagt
    12061 tagcgaacgg taatacgcag ttgactgtaa gtattgctga tgtcagttat tatgtaactg
    12121 aagattcagc tttagataaa gaagcttatg atagagcgac aagtgtatat cttgttgacc
    12181 gtgtgattcc gatgattcca catcgattaa gcaatggtat ttgttcatta aatcctcatg
    12241 ttgatcgttt aacgttaagc tgtcgcatgg aaatcgatgc tagtggtcgc gttgttaaac
    12301 atgaaatttt tgatagtgtt atacattctg attatcgaat gacgtatgat gcggtaaatc
    12361 agattattac tgaaaaggat cctaacattc gcgaacaata taaagaaatt acgcctatgt
    12421 tagatttagc acaagattta tctaatcgtt tgattcaaat gagaaaacga cgtggtgaaa
    12481 tcgattttga tattagtgaa gcaaaagtat tagttaacga agtcggtata ccaacagatg
    12541 ttcaattaag acaacgtggc gagggtgaac gtctaattga atcatttatg ttaattgcaa
    12601 atgaaacagt tgctgaacat tttagtaagt taaatgtacc ttttatttac cgagtgcatg
    12661 agcaacctaa atcagatcgc ttaagacaat tctttgattt tattacaaac tttggcatca
    12721 tgattaaggg cactggcgaa gatattcatc caacaacact tcaaaaggtt caagaagaag
    12781 tagaaggtcg acctgaacaa atggtcattt caacaatgat gttacgttca atgcaacaag
    12841 cgcattatga tgatgtgaac ttgggacatt ttggcttatc agctgaatat tatacgcatt
    12901 ttacatcacc aattagacgt tatcctgatt taacagttca tcgtttaatc cgtaagtatt
    12961 taattgagaa atcaatggat aacaaagaag tgaagcgttg ggaagacaaa ttgcctgagt
    13021 tagctgaaca tacttctaaa cgtgaacgtc gtgctattga ggcagaacgt gatactgatg
    13081 aattgaaaaa agcagaatat atgattcaac atattggtga tgaatttgaa ggtattgtca
    13141 gctcagtagc taacttcggt atgttcattg aattgccaaa tacgatagaa ggtatggttc
    13201 atattgcgaa tatgactgat gattattacc gttttgaaga gcgtcaaatg gcattaattg
    13261 gtgagcgtca agctaaagta tttagaattg gtgacacagt taaggttaaa gtgacgcatg
    13321 ttgatgtaga tgaacgatta attgattttc aaattgttgg aatgccttta cctaaaaatg
    13381 accgctcaca gcgaccagca agaggtaaaa cgattcaagc taaaacgcgt ggcaaatctt
    13441 tagataaatc gaaatctgat gataagggtc gtaagaaaaa aggtaagcaa cgtaaaggta
    13501 aaaaccaacg taataatgat aaatcaggta atagtaagca taagccattt tataaagata
    13561 aaagtgtgaa aaagaaagca cgtcgtaaga aaaaataagc agcaatgagg tgagtatgaa
    13621 tggctaagaa gaaatcacca ggtacattag cggaaaatcg taaagcaaga catgattata
    13681 atattgaaga tacgattgaa gcgggaatcg tattacaagg tacagaaata aaatcgattc
    13741 gccgaggtag tgctaacctt aaagatagtt acgcgcaagt taaaaacggt gaaatgtatt
    13801 tgaataatat gcatatagca ccatacgaag aagggaatcg ttttaatcac gatcctcttc
    13861 gttctcgaaa attattattg cataaacgtg aaatctttaa attaggtgaa caaactcgag
    13921 aaattggtta ttcgattgtg ccgttaaagc tttatttgaa gcatggacat tgtaaagtat
    13981 tacttggtgt tgcacgaggt aagaaaaaat atgataaacg tcaagctttg aaagaaaaag
    14041 cagtcaaacg agatgttgcg cgcgatatga aagcccgtta ttaagcgatt tagttgctta
    14101 atcgggctat atttgatata gttatatgtg cttttgtaaa ttacaaaagt atgatttgtt
    14161 tgatttatta tttcggggac gttcatggat tcgacagggg tcccccgagc tcattaagcg
    14221 tgtcggaggg ttggcttcgt catcaacaca cacagtttat aataactggc aaatcaaaca
    14281 ataatttcgc agtagctgcc taatcgcact ctgcatcgcc taacagcatt tcctatgtgc
    14341 tgttaacgcg attcaacctt aataggatat gctaaacact gccgtttgaa gtctgtttag
    14401 aagaaactta atcaagctag catcatgttg gttgtttatc acttttcatg atgcgaaacc
    14461 tttcgataaa ctacacacgt agaaagatgt gtatcaggac ctctggacgc gggttcaaat
    14521 cccgccgtct ccattatata gtctgcgacc ttagtggttg taggcttttt atttttgagt
    14581 gcacaaaaag tgcacagata gtgcacagga tacaaaaaga cacaaccaat tttagttggc
    14641 agtgccttat aagtatttaa ctatttcttt ctgtgtacta ggatataaat gaccatatcg
    14701 gttatatact tcagtcgtat cgctgtgacc taaacgctga gctataatca ttacactagc
    14761 ccctagatta actagcatag atgcatggct atgtcttaac tcgtgtataa cgattctagg
    14821 gaaattttgt ccgtttggta attgctcatc caacactttt aatgcgttag taaaccaacg
    14881 atcaatagta gactcgctgt aagctttata gaatgtgcca aacaatacat agtcgtcctt
    14941 gtatatgtta ttctctttat accaaattaa ataatctttg atgtcataca tcatgtgagt
    15001 aggtaaatat atatcacgta tggctgattt cgttttaggg gctgtcactt caccgtgata
    15061 gtctgttttg tttatatgga taaagtcatc atcaaagtta atatcgcgcc atgtgagagc
    15121 tctaatttcg ccctttcttg ctccagagta gaacaatagt ttgaaaaata atttttgttg
    15181 ttgtgtagca agcgcctcat agaattgatt gaattgttct aacgtccaat aattcaatcg
    15241 tttctgtgat tctatttcaa aattccctac aagagatgca acattttgtt ttaactcatg
    15301 aaacttcatt gcatgattca gtaatgatac taaatataca tgcatcttct ttaagtagtc
    15361 tccagaatgt ccctctttta atttcttatt ctgaaacttc atcacatctt gtgtagtcat
    15421 tttaaataca tccatagatt taaaataagg tagcaaatgg ttattggtat gtgtctttaa
    15481 tgcttttaca cttgatgtct ttcgacgtgc tgaataccag tctatatatt cttcaacgag
    15541 cttgtcaaaa ggtaatctat taaggtgtcc gactccctct aattcgtcca tcatttcgtt
    15601 acatttcttt actgcatctt tacgttgttt aaacccctta cgtgttatgt acttacgagt
    15661 attgcttttg tcatagtaag tgatacggaa atagtaagtg ccaagtttag cgtctttata
    15721 tatgttgtgg gataagttta agttatatga cattgtattc actcttcact aattttctct
    15781 agatcatgtt taactctata gtcatcaatg tattctaata gatcaaagta tttgttagat
    15841 attgtcgtaa ggaattcatc gatgctctca tcttttaaaa attcttttgc ttttgaatct
    15901 tcataatagt caactactaa ttcttgcgca tttattgttc tttgataagc ga
//
LOCUS       DAHHSC010000043        15949 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.043, whole genome shotgun sequence.
ACCESSION   DAHHSC010000043 DAHHSC010000000
VERSION     DAHHSC010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 15949)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15949)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15949
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.043"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            1..94
                     /locus_tag="NBI57_001047"
                     /pseudo
     CDS             1..94
                     /locus_tag="NBI57_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501402.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
     gene            476..736
                     /locus_tag="NBI57_001048"
     CDS             476..736
                     /locus_tag="NBI57_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1822009.1"
                     /translation="MIIMRGYNERYCSWTNTAIQCDYFNKVRQVKKDGKSDRLSNNIN
                     SNIIYRVLEKCITLYNVCSRCKRALILSEHSKKNIFNIKKAV"
     gene            746..1243
                     /locus_tag="NBI57_001049"
     CDS             746..1243
                     /locus_tag="NBI57_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HCD1822010.1"
                     /translation="MNNRNVYDIEVSDYKGLTYKLEAFRGKVILVVNTATECIYSEQL
                     KKLETLFQKYKDRGFVVLSFPNNNFDNRQPGSNEEILKISREKFGVTFPVLAKISVNG
                     NDEHPLFTHLKNEQPGIFGSPIKWNFTKFIIDRQGNVVNRFLPMEDPLDISTNIEILL
                     QESSS"
     gene            complement(1351..1443)
                     /locus_tag="NBI57_001050"
     CDS             complement(1351..1443)
                     /locus_tag="NBI57_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1822011.1"
                     /translation="MSVINAYSNASLLFFIYLLFIMYQISLSVK"
     gene            1637..3517
                     /locus_tag="NBI57_001051"
     CDS             1637..3517
                     /locus_tag="NBI57_001051"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="assimilatory sulfite reductase (NADPH)
                     flavoprotein subunit"
                     /protein_id="HCD1822012.1"
                     /translation="MKLNTSNSPFTEKQVTEINNLLQTLTESQQQWLSGFLLANSNDT
                     TSDSNQQQLETEVWQQSQISEEQATSTTYMLQNKEPHIEANQRHVTVLYGSESGNAMR
                     LAEIFSERLSDIGHQVVLMSMDEYDTTNIAQLEDLFIITSTHGEGEPPDNAWDFFEFL
                     EDDNAPNLNHVRYSVLALGDQTYEFFCQAGKDVDALLENLGAERICKRVDCDIDYEED
                     AEKWMADIINMIDTTSEGIQSESVISESIKSAKEKKYSKLNPYQAEVLANINLNGTDS
                     NKETRHIEFLLDDFSESYEPGDCIVALPQNDPELVEKLISMLGWDPQSPVPINDRGDT
                     VPIVEALTSHFEFTKLTLPLLKNADIYFDNEELSERIQDESWAREYVINRDFIDLITD
                     FPTIELQPENMYQILRKLPPREYSISSSFMATPDEVHITVGTVRYQAHGRERKGVCSV
                     HFAERIKPGDIVPIYLKKNPNFKFPMKQDIPVIMIGPGTGIAPFRAYLQEREELGMTG
                     KTWLFFGDQHRSSDFLYEEEIEEWLENGNLTRVDLAFSRDQEHKEYVQHRIMEESERF
                     NEWIEQGAAIYICGDEKCMAKDVHQAIKDVLVKERHISQEEAELLLRQMKQQQRYQRD
                     VY"
     gene            3580..4185
                     /locus_tag="NBI57_001052"
     CDS             3580..4185
                     /locus_tag="NBI57_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding protein"
                     /protein_id="HCD1822013.1"
                     /translation="MNMPLMIDLTNKNVVIVGGGVVASRRAQTLNQYVEHMTVISPTI
                     TEKLQNMVDNGVVIWKEKEFEPSDIVDAYLVIAATNEPRVNEAVKQALPEHALFNNVG
                     DASNGNVVFPSALHRDKLTISVSTDGASPKLTKSIMAELEALYPPSYSSYIDFLYTCR
                     QKIKVLDITYNEKQQLLSQIVSQEYLNHDKQAQFLAWLDVR"
     gene            4261..4356
                     /locus_tag="NBI57_001053"
     CDS             4261..4356
                     /locus_tag="NBI57_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1822014.1"
                     /translation="MGGFVNSLIMKDLSDNDMRFKYEYYNREKDT"
     gene            4678..6048
                     /locus_tag="NBI57_001054"
     CDS             4678..6048
                     /locus_tag="NBI57_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CitMHS family transporter"
                     /protein_id="HCD1822015.1"
                     /translation="MNSDNMWLTVMGLIIIISIVGLLIAKKINPVVGMTIIPCLGAMI
                     LGYSVTDLVGFFAKGLDQVINVVIMFIFAIIFFGIMNDSGLFKPLVKRLILMTRGNVV
                     IVCAMTALIGTIAQLDGAGAVTFLLSIPALLPLYKALNMNKYLLILLLALSAAIMNMV
                     PWGGPMARVAAVLKAKSVNELWYGLIPIQIIGFILVMLFAVYLGFKEQKRIKKAKERN
                     ELPQTQDIDVHKLVEVYERDQDVRFPVKGRARTKSWIKWVNTALTLAVILSMLINIAP
                     PEFAFMIGVSLALVINFKSVDEQMERLRAHAPNALMMAAVIIAAGMFLGVLNETGMLK
                     AIATNLIKVIPAEVGPYLHIIVGLLGVPLDLLTSTDAYYFAVLPIVEQTAGQFGVPSV
                     STAYSMVIGNIIGTFVSPFSPALWLAIGLAEANMGTYIKYAFFWIWGFAIVMLVIAML
                     MGIVTI"
     gene            6238..8088
                     /gene="nrdD"
                     /locus_tag="NBI57_001055"
     CDS             6238..8088
                     /gene="nrdD"
                     /locus_tag="NBI57_001055"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HCD1822016.1"
                     /translation="MNQIEEALTGLISKDPAIVNENANKDSDTFSTMRDLTAGIVSKS
                     YALNYLLPKHVADAHQRGDIHFHDLDYHPFQPLTNCCLIDAKNMLHNGFEIGNANVTS
                     PKSIQTASAQLVQIIANVSSSQYGGCTVDRVDELLSTYARHNEEQHRNIAKQFVKESE
                     IDRYVDQQVTKDINDAIESLEYEINTLYTSNGQTPFVTLGFGLGTDHLSRKIQQAILN
                     TRIKGLGKDRTTAIFPKLVFSIKKGTNFSPQDPNYDIKQLALKCSTKRMYPDILNYDK
                     LVEILGDFKAPMGCRSFLPSWKDAEGHFENNGRCNLGVVTLNLPRMALESAGNMTKFW
                     EIFYERIDVLHDALLYRINRLKDAVPNNAPILYKSGAFNYKLKETDDVAELFKNKRAT
                     ISMGYIGLYETATVFYGPDWETSQEAKAFTLEILKEMKRYQTKWTELYDIWFSIYSTP
                     SESLTDRFCRLDQERFGDIKDITDKGYYQNSFHYDVRKDVTPFEKLDFEKDYPYYASG
                     GFIHYCEYPKLQHNLKALEAVWDYSYDKVGYLGTNIPIDHCYECDYDGDFEATEKGFK
                     CPNCGNDNPKTVDVVKRTCGYLGNPVQRPVIKGRHKEICARVKHMKAPKE"
     gene            8085..8621
                     /gene="nrdG"
                     /locus_tag="NBI57_001056"
     CDS             8085..8621
                     /gene="nrdG"
                     /locus_tag="NBI57_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HCD1822017.1"
                     /translation="MTLLDIKQGQGYIAKIESNSFVDGEGVRCSVYVSGCPFNCVGCY
                     NKASQKFRYGEKYTDEILAEILDDCDHDYISGLSLLGGEPFCNLDITLNLVKAFRARF
                     GNTKTIWVWTGFLYEYLANDCTERRELLSYIDVLVDGLFIQHLFKPDLPYKGSLNQRI
                     IDVQQSLSHARMIEYIVS"
     gene            8721..8831
                     /locus_tag="NBI57_001057"
     CDS             8721..8831
                     /locus_tag="NBI57_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1822018.1"
                     /translation="MIEYDLTIILIPILKVAYPFFKKVERKKWYFSGKEV"
     gene            complement(8847..9007)
                     /locus_tag="NBI57_001058"
                     /pseudo
     CDS             complement(8847..9007)
                     /locus_tag="NBI57_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501391.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            9139..10761
                     /locus_tag="NBI57_001059"
     CDS             9139..10761
                     /locus_tag="NBI57_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501390.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BCCT family transporter"
                     /protein_id="HCD1822019.1"
                     /translation="MKKEKVMDWTTFIGTVAVLLFAVIPMMAFPKASEDIITGINSAI
                     SDSIGSIYLFMGLAIFCFVMYIAFGKYGNVTLGKASDKPEFNTFTWAAMLFCAGIGSD
                     ILYWGVIEWAFYYQVPPNGAKSMSDEALQYATQYGMFHWGPIAWAIYVLPALPIGYLV
                     FVKKQPVYKISQACRPILKGQTDKFVGKVVDILFIFGLLGGAATSLALGVPLISAGIE
                     RLTGLDGKNMILRSAILLTITVIFAISSYTGLKKGIQKLSDINVWLSFVLLAFIFIIG
                     PTVFIMETTVTGFGNMLRDFFHMATWLEPFGGIKGRKETNFPQDWTIFYWSWWLVYAP
                     FIGLFIARISKGRRLKEVVLGTIIYGTLGCVLFFGIFGNYAVYLQISGQFNVTQYLNT
                     HGTEATIIEVVHHLPFPSLMIVLFLVSAFLFLATTFDSGSYILAAASQKKVVGEPLRA
                     NRLFWAFALCLLPFSLMLVGGERALEVLKTASILASVPLIVIFIFMMISFLIILGRDR
                     IKLETRAEKLKEVERRSLRIVQVSEEEQDDNL"
     gene            complement(10932..11495)
                     /locus_tag="NBI57_001060"
     CDS             complement(10932..11495)
                     /locus_tag="NBI57_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GbsR/MarR family transcriptional regulator"
                     /protein_id="HCD1822020.1"
                     /translation="MTRSQNKQQQLEQAKDIVINSIGQTMDLYGTNRSVGNLYGTMVF
                     EGSMTLDEMRHQLQMSKPSMSAGVKKLQEFDIVKQQFTRGSRKQHFIAEKDFFIFFRN
                     FFTKKFQREIDINVEAVKDAQAIINPLLESSDLTEAETQEAQKIKAQLDHTHVYYEWL
                     EQLTEAIESGEIFKYFPIPEQPSDSEN"
     gene            complement(11584..11679)
                     /locus_tag="NBI57_001061"
     CDS             complement(11584..11679)
                     /locus_tag="NBI57_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1822021.1"
                     /translation="MKNVSIYKTTQELSFVSSLDFYLTKHLTLML"
     gene            11748..13238
                     /gene="betB"
                     /locus_tag="NBI57_001062"
     CDS             11748..13238
                     /gene="betB"
                     /locus_tag="NBI57_001062"
                     /EC_number="1.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="betaine-aldehyde dehydrogenase"
                     /protein_id="HCD1822022.1"
                     /translation="MELLKHLSQRQYIDGEWVESTNKNTRDIINPYNQEVIFTVSEGT
                     KEDAERAILAARRAFESGEWSQETAEKRGKKVRAIADKIKEHREALARLETLDTGKTL
                     EESYADMDDIHNVFMYFAGLADKDGGEMIDSPIPDTESKIVKEPVGVVTQITPWNYPL
                     LQASWKIAPALATGCSLVMKPSEITPLTTIRVFELMEEVGFPKGTINLILGAGSEVGD
                     VMSGHKEVDLVSFTGGIETGKHIMKNAANNVTNIALELGGKNPNIIFDDADFELAVDQ
                     ALNGGYFHAGQVCSAGSRILVQNSIKDKFEQALIDRVKKIKLGNGFDADTEMGPVIST
                     EHRNKIESYMDVAKAEGATIAVGGKRPDRDDLKDGLFFEPTVITNCDTSMRIVQEEVF
                     GPVVTVEGFETEQEAIQLANDSIYGLAGAVFSKDIGKAQRVANKLKLGTVWINDFHPY
                     FAQAPWGGYKQSGIGRELGKEGLEEYLVSKHILTNTNPQLVNWFSK"
     gene            13499..15208
                     /gene="betA"
                     /locus_tag="NBI57_001063"
     CDS             13499..15208
                     /gene="betA"
                     /locus_tag="NBI57_001063"
                     /EC_number="1.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline dehydrogenase"
                     /protein_id="HCD1822023.1"
                     /translation="MSNKNKSYDYVIIGGGSAGSVLGNRLSEDKDKEVLVLEAGRSDY
                     FWDLFIQMPAALMFPSGNKFYDWIYSTDEEPHMGGRKVAHARGKVLGGSSSINGMIYQ
                     RGNPMDYEGWAEPEGMETWDFAHCLPYFKKLEKTYGAAPYDKFRGHDGPIKLKRGPAT
                     NPLFQSFFDAGVEAGYHKTPDVNGFRQEGFGPFDSQVHRGRRMSASRAYLHPAMKRKN
                     LTVETRAFVTEIHYEGRRATGVTYKKNGKLHTIDANEVILSGGAFNTPQLLQLSGIGD
                     SEFLKSKGIEPRVHLPGVGENFEDHLEVYIQHKCKEPVSLQPSLDIKRMPFIGLQWIF
                     TRTGAAASNHFEGGGFVRSNNEVDYPNLMFHFLPIAVRYDGQKAAVAHGYQVHVGPMY
                     SNSRGSLKIKSKDPFEKPSIRFNYLSTEEDKKEWVEAIRVARNILSQKAMDPFNGGEI
                     SPGPEVQTDEEILDWVRRDGETALHPSCSAKMGPASDPMAVVDPLTMKVHGMENLRVV
                     DASAMPRTTNGNIHAPVLMLAEKAADIIRGRKPLEPQYIDYYKHGVHDENEGAIEVKP
                     YAK"
     gene            15486..15698
                     /locus_tag="NBI57_001064"
     CDS             15486..15698
                     /locus_tag="NBI57_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sterile alpha motif-like domain-containing
                     protein"
                     /protein_id="HCD1822024.1"
                     /translation="MSFYEYIQTFKDDKTPLGELAIWIKEDDSFPKQEKLTENILSYF
                     HQMSNIDHEFLEIVKRSLSLYDQLKS"
BASE COUNT         5565 a         2306 c         3089 g         4989 t
ORIGIN      
        1 gtgtctaaat attggcggtc aatattactt ataaacacga atgttaagta atgtagacaa
       61 tgctgaaaaa acaataagta gaaactctat atagaatgtt cgtttttatt tttaataatt
      121 gaataatcat tattgatgat ttaatatgta ttcggtcgca cacgatacaa agtgacttgt
      181 tttaatgata tgcaataata tgacgtttat cgtcgcgtgt aacaatgtat gagtgtgtca
      241 gtttaatttt aaaagggtta taaggatatt taataagata aaggagggct taataaacaa
      301 tcacatgtga agtttttaag gattatatga gttgggcagc tctttaatcc atagttataa
      361 cgaattttaa attgttagat ctaacgtatg gtatcagtat gtatgagttt aacttaagta
      421 ttaatctata gtttaaagag cggtctaatt gtctttttaa aaatagaaaa taaggatgat
      481 tataatgagg ggttataacg aaagatattg tagctggact aatacagcta tacaatgtga
      541 ttattttaat aaagtaagac aggtgaaaaa agacggtaaa tcggataggt tgagcaacaa
      601 cataaactcg aacattattt atcgtgtgct cgaaaaatgt atcacgctat ataatgtatg
      661 ttcgcggtgt aaacgcgcac ttattttatc agaacattct aaaaagaata tattcaatat
      721 aaaaaaggct gtgtaggggt gtcttatgaa taatagaaat gtgtatgata tcgaagtaag
      781 tgattataaa ggcttaactt ataaattaga agcatttaga ggtaaagtga ttttagttgt
      841 taatactgca acagaatgta tatatagcga acaattgaaa aaactagaga ctttgtttca
      901 aaaatataag gatcgtgggt ttgtagtgtt gagttttccg aataataatt ttgacaatcg
      961 acaaccaggt tctaatgaag aaatcttgaa aattagtcgt gagaaatttg gtgttacatt
     1021 tccagtgcta gctaaaatat ctgtgaacgg gaacgatgaa catccgttat ttacgcattt
     1081 aaagaatgaa caaccaggaa tctttgggtc cccaataaaa tggaatttca caaaatttat
     1141 aatcgatcga caaggcaatg ttgtaaatcg attcttgcca atggaagatc cattggatat
     1201 atcgacaaat atagaaatat tattgcaaga atcttcatct taaatttaaa attgagcgct
     1261 tagtatgcaa ataacacaag agcctcttaa tgatgtacta atagtatgtc attaggaggc
     1321 attttatatt tagaaaatca gtaatttgac ctattttact gaaaggctga tttggtacat
     1381 aataaatagt aaataaataa aaaataacaa cgatgcatta ctgtatgcat taataactga
     1441 cacgacataa tcaaagtttt gtctgaatta ctgataatgt cattcaagca acatgaaagt
     1501 tgtaaatatt tgcaagtata actatcagac acaactcaag tttgttttaa tttataccat
     1561 acatattaaa aaattgacta aactatcgat aaaaaaagag tcaatcatct atttcaagtt
     1621 tattgaggtg aaacatttga aattaaatac atctaatagt ccatttacag aaaaacaagt
     1681 cacagagatt aacaatctgc tgcaaacatt aacagagagc caacaacagt ggcttagtgg
     1741 ttttttacta gctaatagta acgatacgac aagtgatagt aatcaacaac aattagagac
     1801 agaagtgtgg caacaatcac aaatatcaga agaacaagca acttcaacaa cgtatatgtt
     1861 acaaaataaa gagccacata tcgaagctaa tcagcggcat gttacagtgc tatatggttc
     1921 tgaatcaggt aatgccatgc gtttagctga aattttttca gaacgtttaa gtgatatcgg
     1981 acatcaagtt gttttgatgt caatggatga atatgatacg acaaacatcg cacagttaga
     2041 agatttattt attattacgt ctactcatgg tgaaggagaa ccgcctgata atgcatggga
     2101 tttctttgaa tttttagaag acgataacgc acctaattta aatcatgtga gatattcagt
     2161 actagcttta ggtgatcaaa catatgaatt tttctgtcaa gctggtaaag atgtagatgc
     2221 tttactagaa aatctaggcg ctgagcgtat atgtaagcgt gtagattgtg atattgatta
     2281 tgaagaagac gcagaaaagt ggatggcaga catcattaat atgattgata ctacatcaga
     2341 aggtattcaa agtgaatcgg tgataagtga atcaattaag tctgccaaag aaaagaaata
     2401 ttctaaatta aatccatacc aagcagaagt tttagcgaat atcaatttaa atggtaccga
     2461 ttcaaataaa gaaacacgac atatagaatt tttacttgat gattttagtg aatcatatga
     2521 accaggagat tgtatagtag cattaccgca aaacgaccct gaattggttg aaaaactaat
     2581 atccatgtta ggttgggatc cgcaatctcc ggtgccaatt aatgatcgtg gtgatacagt
     2641 tcctattgtt gaagcactaa catcacattt tgaatttact aaattaacat tgccattatt
     2701 gaaaaatgca gatatctatt ttgacaatga agaattatct gaacgtattc aagatgagtc
     2761 atgggcgcgt gaatatgtta taaatcggga ctttatagat ttaataacag attttccaac
     2821 tatagaatta caacctgaga atatgtatca aatccttaga aaattaccac caagagagta
     2881 ttcgatttct agtagtttta tggcaacgcc agatgaagtg catattaccg ttggtacggt
     2941 tcgttatcaa gcacatggac gtgagagaaa aggtgtatgc tcggttcatt ttgctgagcg
     3001 aattaaacca ggcgatatag taccaattta tttgaagaaa aatccgaact tcaaatttcc
     3061 gatgaagcaa gatataccgg ttattatgat tggaccaggt actggaattg ctccttttag
     3121 agcatattta caagaacgtg aagaacttgg tatgactgga aaaacatggt tgttctttgg
     3181 tgatcaacac cgtagttctg actttttata tgaagaagaa atagaagaat ggcttgaaaa
     3241 tggaaactta acacgcgtag atttagcatt ttcaagagac caagaacaca aagaatatgt
     3301 acagcatcgt ataatggaag aaagtgaacg tttcaatgaa tggattgagc aaggcgcagc
     3361 aatctatatt tgtggcgatg aaaaatgtat ggcgaaagat gtccatcaag ccattaaaga
     3421 tgtattggta aaagaacgtc atatttctca agaagaagca gagttattat tgcgacaaat
     3481 gaaacaacaa caacgctatc aacgtgatgt ttattagcga ttggtgttaa atattttaag
     3541 gtgtaatgat gtaaaaagat ataaaggatg ttgctcaaca tgaatatgcc attaatgata
     3601 gatttaacaa ataaaaatgt cgtcatagtt ggtggaggcg tcgttgcaag tcgtcgggca
     3661 caaacattaa atcaatacgt tgaacatatg acggtcatca gtccgacaat cactgaaaaa
     3721 cttcaaaata tggtagataa cggtgtcgtc atatggaaag aaaaagaatt tgaaccaagc
     3781 gatattgtag acgcgtatct agttattgca gcaaccaatg agccacgtgt caatgaagcg
     3841 gtaaaacaag ccttacctga gcatgccctt tttaataatg ttggagatgc atcaaatggc
     3901 aatgttgtat ttccaagtgc actacaccgc gacaagctaa ctatcagtgt atcaactgat
     3961 ggtgcgagtc ctaagttgac aaaatcaatt atggcagagc ttgaggcgtt atatccacca
     4021 tcatacagtt cgtatatcga ctttttatat acttgccgac agaaaataaa agtacttgat
     4081 ataacatata acgaaaagca acagttactg tcacaaattg tgtcacaaga atatttaaat
     4141 catgacaaac aagctcaatt tttagcgtgg ttggatgtaa gataataata gcggaccgtc
     4201 taaccgtcta aggtaagtct tcttatttta actttaacgc ttaatcattg aaattaagac
     4261 atgggtggct ttgtgaatag tctaataatg aaggatttaa gcgataatga tatgcgtttt
     4321 aaatatgaat attacaatag agaaaaagat acgtagaaca aacttaataa aatagatgga
     4381 taaattgaaa tctggttgaa gtcgttacta tcatagcgac ctttagccag attttttgtg
     4441 caatagaaag caataataaa aatgatagat caaaatgaaa tacaggacag gatatacaag
     4501 gattagtcat gccatgttat caagtaggaa aatcaaactt cactattgat agttacgcaa
     4561 aaaagatttt tttgataaaa tgagataact taaatataaa aaaatatatt aattataata
     4621 tttaagttaa agaggggtat tatgtaaatt gtattaaaag tggagggaga aaataatatg
     4681 aatagtgata atatgtggtt aacagtaatg gggctcatta ttattatttc aattgtaggt
     4741 ttactcattg ccaaaaagat aaatccagtt gtaggtatga caatcatacc ttgcttaggg
     4801 gcaatgattt taggatatag tgtgacagat ttggttggat tttttgctaa agggttagat
     4861 caagtcatca acgttgttat tatgtttatc tttgccatta ttttctttgg catcatgaac
     4921 gatagtggtt tattcaagcc gcttgtcaaa cgcttaatat taatgacacg aggcaatgtc
     4981 gtcattgtct gtgcaatgac agctttaatt ggcacaatag cccaattaga tggggccggt
     5041 gcggtaacat ttttgctttc tattcctgca ttattacctt tatataaagc gttaaatatg
     5101 aataaatatt tattgatttt actattagca ttaagcgcgg cgattatgaa catggtacct
     5161 tggggaggtc caatggctcg tgtagctgca gtgttaaaag ccaaaagtgt caatgaatta
     5221 tggtatggat taatacctat tcaaataata ggtttcattc ttgttatgtt gtttgcggta
     5281 tatcttggat ttaaagaaca gaaacgtatc aaaaaagcaa aagagagaaa tgaattaccg
     5341 caaacacaag atatagatgt acataaatta gttgaagtat atgaacgaga tcaagatgta
     5401 aggtttcctg taaaaggacg tgcaagaaca aaatcatgga taaaatgggt gaatacagct
     5461 ttaactttag ctgttattct atcgatgtta ataaatattg cgccacctga atttgcattc
     5521 atgataggtg tctcattggc gcttgttatt aattttaaat cagtggatga acaaatggag
     5581 cgattaagag cgcatgcgcc gaatgcatta atgatggctg cagtgattat tgcagcaggt
     5641 atgtttttag gtgtactaaa tgaaaccggt atgcttaaag cgattgcgac caatttaatc
     5701 aaagtgattc ctgcagaagt aggaccatac ttgcatatta ttgtaggttt acttggcgta
     5761 ccattagatt tactaactag cacagacgct tattattttg ctgtgttacc gattgtcgaa
     5821 caaacagcag ggcaatttgg tgtaccgtct gtatcaacag cttattcaat ggtcataggg
     5881 aatattatag gtacatttgt cagcccattt tcaccagcct tatggttggc aattggttta
     5941 gcagaggcaa acatgggcac gtatattaag tatgcattct tttggatttg gggattcgcc
     6001 atcgttatgt tagtaattgc aatgttgatg ggcattgtga cgatttaagt tgcataaaca
     6061 tgtcgaaatg acagaacaat tttaaaaatt aatgaaataa ttgtgtagaa atatgaattt
     6121 cactaaatgt taataacttt gtgacgtttt agttaacaga ctaataaaaa tttgaaaata
     6181 ctatatatag tggtataacg taatgagtag acacaatata taggaagaag gggtaaaatg
     6241 aatcaaatcg aagaagcatt aacgggtttg atttctaaag atcctgctat tgttaacgaa
     6301 aatgccaaca aagatagtga tacattttca acaatgagag atttaacagc aggtatcgtt
     6361 tctaaatctt acgcattaaa ttatttatta ccaaagcacg ttgcagatgc acatcaaaga
     6421 ggggacatac attttcacga cttagattat catccattcc aaccgttaac aaactgttgt
     6481 ttaatagatg ctaaaaatat gctacataat ggatttgaaa taggcaacgc gaatgtaact
     6541 tcaccaaaat caatacaaac tgcatcagcg cagcttgtac aaattatagc caatgtttcc
     6601 agcagtcaat atggtggctg tacggttgac cgcgttgacg aattacttag tacatatgca
     6661 cgacataatg aagaacaaca tagaaatatc gcaaagcaat ttgtcaaaga atctgaaatt
     6721 gatcgttatg ttgatcaaca agtcactaaa gacatcaatg atgcgattga aagtctagaa
     6781 tatgaaatta ataccttata tacatctaat ggacagacac cttttgtaac attaggattc
     6841 ggcttaggta cagatcattt aagtcgcaaa attcaacaag ctatcttaaa tactcgtatc
     6901 aaaggcttag gaaaagaccg cacgacagcg attttcccaa aacttgtatt ttcaattaaa
     6961 aaaggaacca actttagtcc gcaagatccg aactatgaca ttaaacaact agcattaaag
     7021 tgttcaacga aacgtatgta tccagatatt ttaaattatg acaaactcgt agaaatatta
     7081 ggtgatttca aagcgccaat gggttgtcgt tcatttttac caagttggaa agatgcggaa
     7141 ggtcattttg aaaataatgg tcgttgtaat cttggtgttg ttacacttaa tttacctaga
     7201 atggcattag aatctgccgg taatatgacg aaattctggg aaatctttta tgaacgtatc
     7261 gatgtgttac atgatgcatt actttatcgt ataaatcgtt tgaaagatgc tgtaccgaat
     7321 aacgcaccaa ttttatataa aagtggcgct tttaactata aattaaaaga aacagatgat
     7381 gttgctgagt tatttaaaaa taaacgtgca acgatttcaa tgggctatat agggttgtat
     7441 gaaacagcta ctgttttcta tggtccagac tgggaaacat ctcaagaagc aaaagcattt
     7501 acgcttgaaa ttcttaaaga aatgaaacgt tatcaaacga aatggacaga attatatgac
     7561 atttggttca gtatttacag tacgccgagt gaatcgctaa cggatcgttt ttgtcgttta
     7621 gaccaagaga gatttggaga tattaaagac attacagata aaggatatta tcaaaactct
     7681 ttccattatg atgtacgtaa agatgttaca ccttttgaaa agttagattt tgaaaaagat
     7741 tatccttatt atgcgagtgg tggtttcatt cactattgtg agtatccgaa attgcaacac
     7801 aatttgaaag cactagaagc ggtatgggac tactcttatg acaaagttgg ttacttaggt
     7861 acaaatattc cgattgatca ttgttatgaa tgtgattacg atggagattt tgaagcaact
     7921 gaaaaaggat ttaaatgccc gaactgtggc aatgataatc ctaaaacagt tgatgtcgtt
     7981 aaacgaacat gtggttactt aggcaatcca gttcaacgtc cagtaattaa aggccgtcat
     8041 aaagaaattt gcgcacgagt aaaacatatg aaagcgccta aagaatgaca cttttagaca
     8101 ttaaacaagg acaaggttat attgctaaaa tagaatcaaa tagctttgtt gacggtgaag
     8161 gagtaagatg cagtgtttat gtatcaggat gtccatttaa ttgtgttgga tgttataaca
     8221 aagcctcaca aaagttcaga tatggcgaga aatacactga tgaaatatta gcagaaatat
     8281 tagatgattg cgatcatgat tatatatctg ggctaagtct attaggtggc gaaccatttt
     8341 gtaatttgga tattacatta aatcttgtca aagcatttcg agcacgtttt ggaaatacaa
     8401 agacaatttg ggtatggact ggatttttat atgaatattt agcaaatgat tgtacagaac
     8461 gtcgagagtt attatcatac attgacgttt tagtagatgg tctatttata caacacttat
     8521 tcaaacctga tttaccatat aaaggttctt taaatcaacg cattatagat gtacaacaat
     8581 cactctcgca tgcgcgtatg attgaatata tagttagttg aatatgtatt agaagtcaag
     8641 gtaacattcg ttgccttggc ttctttttag gttaggtaca taattgaaag ttaataaaag
     8701 caattcttta gaaaaatata ttgatagaat atgacctaac aatcattttg ataccaatac
     8761 taaaagttgc atatccgttt tttaaaaaag ttgaaagaaa aaagtggtac tttagtggga
     8821 aggaagtcta actttttggt agcgttttac aataaataaa tattcgttaa aaacgtataa
     8881 atattcttaa atgccgtcct agtaaatttg ttaaattcgt taaatcataa cttaacacag
     8941 ctgttttagc gctattaaag ttttgtttat tacgagaaaa attatataaa tattcaataa
     9001 ttgccaagtt tcaaattgta tgaaatttgc attattatta aatattagtt attgtcaatt
     9061 ttgtgaatca atataattat tacattttga gataaatcga aacaggattc ataaaattaa
     9121 taattagggg gagcacaatt gaaaaaagag aaagttatgg actggacgac ctttataggg
     9181 acagtagctg tacttctttt tgcagttata cctatgatgg cttttccaaa agcaagtgaa
     9241 gatatcatca ctggtattaa tagtgccatt tctgattcaa ttggttcgat atatttattt
     9301 atggggctgg cgattttttg ctttgtaatg tatatcgcat ttggtaaata tggtaatgtc
     9361 acacttggta aagcaagtga taaaccagaa tttaatacat ttacatgggc ggcaatgctg
     9421 ttttgtgcag gcataggctc tgatatttta tactggggcg ttattgaatg ggctttttac
     9481 tatcaagttc caccaaatgg cgcgaaaagt atgagtgatg aagcactcca atatgcgacg
     9541 caatatggta tgttccactg gggaccaatt gcttgggcta tttacgtttt accagcatta
     9601 ccaattggtt atttagtgtt tgttaaaaaa caaccggtgt ataaaattag tcaagcttgt
     9661 cgtccgattt taaaaggtca aacagataaa tttgtaggta aagttgtaga tatcttattt
     9721 atctttggat tgctaggtgg tgcggcaaca tcactagcgt taggtgtgcc attaatttct
     9781 gcaggcatag aaagattaac tggtttagat ggtaaaaata tgattttacg ttcggctatt
     9841 ttattaacga ttacggtcat atttgctatt agttcatata caggattgaa aaaaggtatt
     9901 caaaagttaa gtgatatcaa cgtttggcta tcatttgtac ttttagcctt tatatttatt
     9961 attggaccga ctgtttttat tatggaaacg acagtgacag ggttcggaaa tatgttgaga
    10021 gattttttcc atatggcaac atggttagaa ccattcggtg gtattaaagg tcgaaaagaa
    10081 acgaatttcc cacaagactg gacaatattc tactggtcat ggtggttagt atatgcgcca
    10141 tttatcggtt tatttatcgc tagaatttca aaaggtagac gccttaaaga agtcgtgcta
    10201 ggaacaatta tttatggaac gcttggatgc gtattattct ttggtatttt tggtaactat
    10261 gctgtgtatt tacaaatttc tggacagttt aatgtaacac aatatttaaa tacacatggt
    10321 acagaggcaa ccattattga agtggtgcat catttaccat tcccatcatt gatgattgta
    10381 ctattcttag tatctgcttt cttattctta gcaacaacat ttgattcggg ttcatatatt
    10441 ttagcggcag catctcagaa aaaagtggta ggcgaaccat tacgtgccaa tcgtttattc
    10501 tgggcatttg cattgtgctt attgccattt tcattgatgc tagttggtgg cgaacgtgca
    10561 ttagaagtat tgaaaactgc ttcaatactg gcaagtgtgc cattaattgt tatttttatt
    10621 ttcatgatga tatcattttt aatcatttta gggcgcgata gaattaaact tgaaacgcgt
    10681 gctgaaaaat taaaagaagt tgaacgtcgt tcattgcgaa tcgttcaagt atcagaagaa
    10741 gaacaagacg ataatttata attcaaataa tgatgcataa gattttgaat tttataagta
    10801 tataattgat tgtcatataa aacaaaaacg gtaatttcta tttattgaga tagaaattac
    10861 cgtttatttc gtggacctat tgtattgttt tcatcatgca taatcatcat tgccgctgtc
    10921 cgaggctaat tttaattttc agaatcagaa ggctgttctg gaattgggaa atatttgaaa
    10981 atttcaccgc tttcaatcgc ttcggttaac tgttctaacc attcgtaata aacatgtgta
    11041 tgatcaagct gagctttaat tttttgtgcc tcttgtgttt cagcttcagt taaatcactg
    11101 ctttcaagta atggattgat aatagcttga gcatctttta ctgcttcgac attgatgtca
    11161 atttcacgct gaaatttttt agtgaaaaag tttcggaaaa agatgaaaaa gtctttctcg
    11221 gcgataaaat gttgtttgcg gcttcctctc gtaaattgtt gtttaacaat atcaaattcc
    11281 tgcaatttct taacgccagc actcatactt ggtttgctca tttgcaattg atgacgcatt
    11341 tcatcaagcg tcatactgcc ttcaaacacc atagtgccat ataagtttcc tacacttcta
    11401 ttagtgccat acaaatccat tgtctgtcca attgaattaa ttacaatatc ttttgcttgt
    11461 tctaattgtt gctgtttgtt ctgagaacga gtcatcattg cacctccgta catcattttg
    11521 ttcacgttaa aataaatact aatacattat aaaacctttt ctaaaaaaag acattaaaaa
    11581 tatttaaagc attaaagtta aatgtttcgt taaataaaaa tctaacgaac ttacaaaact
    11641 taattcttga gttgttttgt aaattgacac atttttcatt tctatgctaa cataagtttg
    11701 taaaattcgt taaataaaaa tttaacaaac ttaacggagg ttgttgaatg gaacttttaa
    11761 aacatttatc tcagcgtcaa tatattgatg gtgagtgggt tgaaagcacg aataaaaata
    11821 caagagatat tatcaatcct tacaatcaag aagtgatatt tacggtttct gaagggacaa
    11881 aagaggatgc agaacgtgca atcttagctg caagacgtgc gtttgagtct ggtgaatggt
    11941 cgcaagaaac tgctgaaaaa agaggtaaaa aagtacgtgc catcgcggac aagatcaaag
    12001 aacatcgcga agcgttagca cgattagaaa cattagatac tggaaaaacg ttagaagaat
    12061 catatgcaga tatggatgat attcataatg tgtttatgta ttttgctgga ttagcagata
    12121 aagatggtgg cgaaatgatt gattcaccaa ttccagatac agaaagcaaa attgttaaag
    12181 aaccagtagg tgtagttaca caaattacac cttggaatta tccgttatta caagcatcat
    12241 ggaaaattgc gccagcgctt gctacgggtt gttcactagt tatgaaacca agtgaaatta
    12301 caccattaac aacaatacgt gtttttgaat taatggaaga agttggtttc cctaaaggaa
    12361 caattaatct tattctaggt gcaggttctg aagttggtga cgtaatgtca ggtcataaag
    12421 aggttgacct tgtatcattt acaggtggca ttgagactgg taagcatatt atgaaaaatg
    12481 ctgctaataa tgttacgaat attgccttgg aacttggcgg taaaaatcca aacattatct
    12541 ttgatgatgc tgattttgaa ttggcagtag accaagcgtt aaatggtgga tattttcatg
    12601 caggtcaagt ttgttcagca ggatcaagaa tattagtaca aaacagtatt aaagacaaat
    12661 ttgagcaagc acttattgat cgcgtgaaaa aaatcaaatt aggtaatggt tttgatgctg
    12721 atactgaaat gggaccagtg atttcaacag aacatcgtaa taagatcgaa tcttatatgg
    12781 atgtagctaa agcagaaggc gcaacaattg ctgttggtgg taaacgtcca gatagagacg
    12841 atttaaaaga tggtctattc ttcgagccaa cagtcattac aaattgtgat acgtcaatgc
    12901 gtattgtaca agaagaggtt ttcggacctg tcgttactgt agaaggcttt gaaactgaac
    12961 aagaagcgat tcaattagcg aatgattcta tatatggttt agcaggtgct gtattttcta
    13021 aagatattgg aaaagcacaa cgcgttgcta acaagttgaa acttggaacg gtgtggatta
    13081 atgatttcca tccatatttt gcacaagcgc catggggtgg atacaaacaa tcaggtatcg
    13141 gtagagaatt aggcaaagaa ggcttagaag agtaccttgt ttcaaaacac attttaacaa
    13201 atacaaatcc acaattagtg aattggttta gcaaataaaa attagataag gtgagtgcca
    13261 ttgtaagaac acaagacact cactttgttt tgtataagtg gcgaaatgtt gattgataat
    13321 ttggactaaa cgcaaaatga atcatagatt atttcatgac tgttagtaac aatcgtaaaa
    13381 ggaaaatcga gtgttttggt tagccaagtt tagcaattca acgataacca atcagccact
    13441 aacaaatatt tcatgcaata ctcactttga aatacaacaa actttggagg tcataacgat
    13501 gagtaacaaa aataaatcat atgattatgt catcattgga ggaggcagtg caggttctgt
    13561 actaggtaat cgtctgagtg aagataaaga taaagaagtc ttagtattag aagcgggtcg
    13621 cagtgattat ttttgggatt tatttatcca aatgcctgct gcgttaatgt tcccttcagg
    13681 caataaattt tacgattgga tttattcaac agatgaagaa ccacatatgg gcggtcgtaa
    13741 agtagcacat gcccgaggta aagttttagg tggatcaagt tcgattaatg gcatgattta
    13801 tcaacgtggt aatccaatgg actatgaagg ctgggcagaa ccagaaggta tggaaacttg
    13861 ggattttgcg cactgtttac cgtattttaa aaaattagaa aaaacatacg gtgcagcgcc
    13921 ttatgataaa tttagaggcc atgatggacc aattaagtta aaacgagggc cagcaacgaa
    13981 tcctttattc cagtcattct ttgatgcagg tgttgaagca ggttatcata aaacacctga
    14041 tgtgaatgga tttagacaag aaggttttgg accgttcgat agtcaagtac atcgtggtcg
    14101 ccgaatgtca gcttcaagag catatttaca tccagcgatg aagcgtaaaa acttaaccgt
    14161 tgaaacacgt gcctttgtaa ctgaaattca ttatgaaggt agaagagcaa ctggtgttac
    14221 gtataagaaa aatggcaaac tacataccat cgatgctaat gaagtcattt tgtctggtgg
    14281 ggcattcaat acgccacaat tactacaatt atctggtatc ggtgattcag agttcctaaa
    14341 atcaaaaggc attgagccac gtgttcattt accaggtgtg ggtgaaaact ttgaagatca
    14401 cttagaggta tacattcaac ataaatgtaa ggaacctgta tcattacagc caagcttaga
    14461 tatcaagcgc atgccattta ttggtttaca atggattttc acacgtacag gtgcagcagc
    14521 atctaaccat tttgaaggtg gcggatttgt acgttcaaat aacgaagttg attatcctaa
    14581 cttaatgttc catttcttac caatagctgt aagatatgat ggtcaaaagg cagcggtcgc
    14641 acatggttat caagttcacg taggaccaat gtattcaaac tctcgtggta gcttgaaaat
    14701 caaatctaaa gatccattcg aaaaaccaag tatccgcttt aattatttat caacagaaga
    14761 agataaaaaa gaatgggtag aagcaattcg tgtagcaaga aatatcttat ctcaaaaagc
    14821 aatggatcca ttcaatggtg gagaaatttc accaggacca gaagtgcaaa cagacgaaga
    14881 aattcttgat tgggttcgta gagatggtga aacagcatta cacccatctt gtagtgctaa
    14941 aatgggtcct gcttcagatc caatggcagt agttgacccg ttaacaatga aagtccacgg
    15001 tatggaaaat ttacgtgtag ttgatgcatc cgctatgcca cgtacgacaa atggcaatat
    15061 acacgcacct gttttaatgt tagctgaaaa agcagcagac attatacgtg gcagaaagcc
    15121 tttagagcca caatatattg attattataa gcatggtgtt catgatgaaa atgaaggtgc
    15181 aatcgaagta aaaccatacg ctaaataaat ttatatagta gactcttagt ctggcatcta
    15241 ttgtattggt gccagacttt tttgcgtttt atctaaatga gaagttaatg tgttgtttaa
    15301 aaacggtgtg tgagtaagtg aactgaaaat agagttaatt gtacattgtg atgattaatt
    15361 gacctaattg gcagacatta aaagagtgta caaccttaaa aagttcacta aatagacatt
    15421 taattgtgtt ttcttgaaag ctttcgtggt aggatttata taagtaaatt gagagaagga
    15481 atactatgag cttttacgaa tatatacaaa catttaaaga tgataaaaca ccattaggcg
    15541 aattagcgat ttggattaaa gaagatgatt cattcccaaa acaagagaag ctaacagaaa
    15601 acattttgtc ttattttcat caaatgtcca atatagatca tgagttttta gaaattgtaa
    15661 aaagatcact ttctctgtat gatcaattaa aatcgtaaaa tcagaaataa agttagctaa
    15721 aataaaaggc cggtgatttc acagatgaag ttgtcggcta ttttcatgac taatgttaaa
    15781 taatgatcat gaaattgaag acacaaaaat agtgggcgac tcctgtcccc atttgcatga
    15841 aatattaata ctttttacaa agtcttatct tttaaaaaga agccaaacta tgattgtaat
    15901 taataaatac gggaatgaat tagtcataga tataaagtaa tatgaccaa
//
LOCUS       DAHHSC010000044        15455 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.044, whole genome shotgun sequence.
ACCESSION   DAHHSC010000044 DAHHSC010000000
VERSION     DAHHSC010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 15455)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15455)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15455
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.044"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            complement(185..913)
                     /locus_tag="NBI57_001065"
     CDS             complement(185..913)
                     /locus_tag="NBI57_001065"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HCD1822025.1"
                     /translation="MTKLFIPYIMGNKDLIENATLLSENGADIIEIGVPFSDPVADGP
                     VIMEAGQQAIKQGITIDYIFNQLEKHGDQIKCNYVLMTYYNIICHYGEQAFFEKCRDT
                     GVYGLIIPDLPYELSQRLKQQFSHYGVKIISLVAMTTDDKRIKDIVSHAEGFIYTVTM
                     NATTGQNGAFHPELKRKIESIKAIANVPVVAGFGIRTPQHVADIKEVADGIVIGSEIV
                     KRFKSNTREEIIRYLQSIQQTLNN"
     gene            complement(906..2120)
                     /gene="trpB"
                     /locus_tag="NBI57_001066"
     CDS             complement(906..2120)
                     /gene="trpB"
                     /locus_tag="NBI57_001066"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HCD1822026.1"
                     /translation="MNKQIQTEADELGFFGEYGGQYVPETLMPAIIELKKAYKEAKAD
                     PEFQRELEYYLSEYVGRATPLTYAASYTESLGGAKIYLKREDLNHTGAHKINNALGQA
                     LLAKRMGKKKLVAETGAGQHGVASATVAALFDMELVVFMGSEDIKRQQLNVFRMELLG
                     AKVVAVEEGQGTLSDAVNKALQYWVSHVDDTHYLLGSALGPDPFPTIVRDFQSVIGKE
                     IKSQILKKEGRLPDAIVACIGGGSNAIGTFYPFIKDDVALYGVEAAGQGEDTDKHALA
                     IGKGSPGVLHGTKMYLIQDEGGQVQLAHSISAGLDYPGIGPEHSYYHDIGRVTFENAS
                     DTQAMNALINFTKHEGIIPAIESAHALSYVERLAPTMSKEDIIVVTISGRGDKDMETI
                     RQYMAERGLAND"
     gene            complement(2113..2745)
                     /locus_tag="NBI57_001067"
     CDS             complement(2113..2745)
                     /locus_tag="NBI57_001067"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HCD1822027.1"
                     /translation="MKLKFCGFTSIKDVTAASQLPIDAIGFIHYEKSKRHQTITQIKK
                     LASAVPNHIDKVCVMVNPDLTTIEHVLSNTSINTIQLHGTESIDFIQEIKKKYSSIKI
                     TKALAADENIIQNINKYKGFVDLFIIDTPSVSYGGTGQTYDWTILKHIKDIPYLIAGG
                     INSENIQTVNQLKLSHQGYDLASGIEVNGRKDIEKMTAIVNIVKGDRDNE"
     gene            complement(2745..3527)
                     /gene="trpC"
                     /locus_tag="NBI57_001068"
     CDS             complement(2745..3527)
                     /gene="trpC"
                     /locus_tag="NBI57_001068"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HCD1822028.1"
                     /translation="MTILAEIVKYKQSLLQNGYYQDKLNTLKSVKIQNKKSFINAIEK
                     EPKLAIIAEIKSKSPTVNDLPERDLSQQISDYEKYGANAVSILTDEKYFGGSFERLQA
                     LTTKTTLPVLCKDFIIDPLQIDVAKQAGASMILLIVNILSDKQLKDLYNYAISQNLEV
                     LIEVHDRHELERAYKVNAKLIGVNNRDLKRFVTNVEHTNTILENKKPNHHYISESGIH
                     DASDVRKILHSGIDGLLIGEALMRCDNLSEFLPQLKMQKVKS"
     gene            complement(3529..4527)
                     /gene="trpD"
                     /locus_tag="NBI57_001069"
     CDS             complement(3529..4527)
                     /gene="trpD"
                     /locus_tag="NBI57_001069"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HCD1822029.1"
                     /translation="MTLLTRIKTETILLESDIKELIDILISPSIGTDIKYELLSSYSE
                     REIQQQELTYIVRSLINTMYPHQPCYEGAMCVCGTGGDKSNSFNISTTVAFVVASAGV
                     KVIKHGNKSITSNSGSTDLLNQMNIQTTTVDDTPNQLNEKDLVFIGATESYPIMKYMQ
                     PVRKMIGKPTILNLVGPLINPYHLTYQMVGVFDPTKLKLVAKTIKDLGRKRAIVLHGA
                     NGMDEATLSGDNLIYELTEDGEIKNYTLNATDYGLKHAPNSDFKGGSPEENLAISLNI
                     LNGKDQSSRRDVVLLNAGLSLYVAEKVDTIAEGIELATTLIDNGEALKKYHQMRGE"
     gene            complement(4533..5099)
                     /locus_tag="NBI57_001070"
     CDS             complement(4533..5099)
                     /locus_tag="NBI57_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HCD1822030.1"
                     /translation="MILVVDNYDSFTYNLVDIVAQHTDVIVQYPDDDNVLNQSVDAVI
                     ISPGPGHPLDDQQLMKIISTYQHKPILGICLGAQALTCYYGGEVIKGDKVMHGKVDTL
                     KVISHHQHLLYQDIPEQFSIMRYHSLISNPDNFPEELKITGRTEDCIQSFEHKERPHY
                     GIQYHPESFATDYGVKIITNFINLVKEG"
     gene            complement(5096..6502)
                     /locus_tag="NBI57_001071"
     CDS             complement(5096..6502)
                     /locus_tag="NBI57_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HCD1822031.1"
                     /translation="MDIFYKKIKANVTPEVLAQLHSKKIILESTNQQQTKGRYSVVIF
                     DIYGTLTLDNDVLSVSTLKESYQIIERPYHYLTTKINEDYHNIQDEQLKSLRFISGYV
                     GTCSFDLVRHEFPKLQSIQLEDHKQHDVRLYMVEQVYVFDHYKDELYIIATNQFSNST
                     KSDLENRVNKSIEDLTKIQPFMPTQDFDFKTKEIQSNISEERFIEMIQYFKEKITEGD
                     MFQVVPSRIYKYAHHASQHLNQLSFQLYQNLKRQNPSPYMYYLNIDQPYIVGSSPESF
                     VSVKDQIVTTNPIAGTIQRGETTQIDNENMKQLLNDPKECSEHRMLVDLGRNDIHRVS
                     KIGTSKITKLMVIEKYEHVMHIVSEITGKINQNLSPMTVIANLLPTGTVSGAPKLRAI
                     ERIYEQYPHKRGVYSGGVGYINCNHNLDFALAIRTMMIDEQYINVEAGCGVVYDSIPE
                     KELNETKLKAKSLLEVRP"
     gene            complement(6994..8025)
                     /locus_tag="NBI57_001072"
     CDS             complement(6994..8025)
                     /locus_tag="NBI57_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HCD1822032.1"
                     /translation="MEPILEMIKTLTGINSPSGDTEEAIQFVEKYAKDLGYQTTLTNK
                     GALLITVPGKNDEVQRCITAHVDTLGAMVKEIKEDGRLAIELIGGFTYNAIEGEYCQI
                     KTDAGQIYTGTICLHETSVHVYRNNHEIPRDQKHMEIRIDEVTTSEEDTKSLGISVGD
                     FVSFDPRTVITSSGFIKSRHLDDKASVAMILQLLKKLKEEQIILPHTTQFYISNNEEI
                     GYGANASIDSKIKEYIALDMGALGDGQASDEYTVSICAKDASGPYHKQLKSHLVNLCK
                     INNIPYKVDIYPYYGSDASAALHAGADIRHGLFGAGIESSHAMERTHIDSIKATEKLL
                     YAYCLSPIE"
     gene            8190..9281
                     /locus_tag="NBI57_001073"
     CDS             8190..9281
                     /locus_tag="NBI57_001073"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HCD1822033.1"
                     /translation="MTTVLFVGLGLIGGSLASNIKYHNPNTNIIAYDADTSQLDKAKS
                     IGIINEKCLNYSEAIKKADVIIYATPVAITNKYLSELIDMPTKPGVIVSDTGSTKAMI
                     QQHESNLLKHNIHLVSGHPMAGSHKSGVLNAKKHLFENAYYILVYNEPRNEQAANTLK
                     ELLSPTLAKFIVTTAEEHDYVTSVVSHLPHIVASSLVHVSQKNGQEHHLVNKLAAGGF
                     RDITRIASSNAQMWKDITLSNKTYILEMIRQLKSQFQDLERLIESNDSEKLLSFFAQA
                     KSYRDALPAKQLGGLNTAYDLYVDIPDESGMISKVTYIMSLHNISISNLRILEVREDI
                     YGALKISFKNPTDRERGMQALSDFDCYIQ"
     gene            complement(9418..10680)
                     /locus_tag="NBI57_001074"
     CDS             complement(9418..10680)
                     /locus_tag="NBI57_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HCD1822034.1"
                     /translation="MYNYHLLEDRDVLCIDQKSFFASVSCIEKGLDPLETKLAVVADT
                     KRQGSVILAATPKLKELGIKTGSRLFEIPHRNDIYIINPSMRKYLNVSVAISKIALRY
                     IPPEDLHQYSIDEFFMDVTDSYHRFSSTVHAFCERLKREIYEETGIYCTVGIGSNMLL
                     SKIAMDVEAKHSQNGIAEWRYQDVPTKLWPIQPLRDFWGINRRTEAKLNKRGIFTIGD
                     LAKYPYKFLKKEFGILGVDMHLHANGIDQSKVREKHKISNPSICKSQILMRDYHFDEA
                     KVVMQELIEDVASRVRARKKVARTIHFAFGYSDEGGVHKQYTLKDPTNLEKDIYKVVM
                     HFADKLCNKQALYRTLSISLSQFINEDERQLSLFEDEYQRKRDECLAKTIDQLHLKYG
                     KGIVSKAVSFTEAGTKHGRLGLMAGHKM"
     gene            10828..11013
                     /locus_tag="NBI57_001075"
     CDS             10828..11013
                     /locus_tag="NBI57_001075"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase"
                     /protein_id="HCD1822035.1"
                     /translation="MPIVNVKLLEGRSDEQLKNLVSEVTDAVEKTTGANRQAIHVVIE
                     EMKPNHYGVAGVRKSDQ"
     gene            complement(11101..12084)
                     /locus_tag="NBI57_001076"
     CDS             complement(11101..12084)
                     /locus_tag="NBI57_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HCD1822036.1"
                     /translation="MDKETNDNEYRRQSEHRTSAPKRKKKKKIRKLPIILLIVVILLI
                     ALVVYIVHSYNSGVEYAKKHAKDVKVHQFNGPVKNDGKISILVLGADKAQGGQSRTDS
                     IMVVQYDFINKKMKMMSVMRDIYADIPGYGKHKINSAYALGGPELLRKTLDKNLGINP
                     EYYAVVDFTGFEKMIDELMPEGVPINVEKDMSKNIGVSLKKGNHRLNGKELLGYARFR
                     HDPEGDFGRVRRQQQVMQTLKKEMVNFRTVVKLPKVAGILRGYVNTNIPDSGIFQTGL
                     SFGIRGEKDVKSLTVPIKNSYEDVNTNTDGSALQINKNTNKQAIKDFLDED"
     gene            12221..12730
                     /gene="msrA"
                     /locus_tag="NBI57_001077"
     CDS             12221..12730
                     /gene="msrA"
                     /locus_tag="NBI57_001077"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HCD1822037.1"
                     /translation="MNINTAYFAGGCFWCMTKPFDTFDGIEKVTSGYMGGHIENPTYE
                     QVKSGTSGHLETVEIQYDVALFSYNKLLEIFFSVIDPLDTGGQYQDRGPQYQTAIFYT
                     NDHQKELAETYIEQLKNTINADKAIATKILPASQFYKAEDYHQDFYKKNPERYAEEQK
                     IRQEYKNKQ"
     gene            complement(12933..15455)
                     /gene="mprF"
                     /locus_tag="NBI57_001078"
     CDS             complement(12933..15455)
                     /gene="mprF"
                     /locus_tag="NBI57_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HCD1822038.1"
                     /translation="MNQEVKNKIFSILKITFATALFIFVAITLYRELSGINFKDTLVE
                     FSKINRMSLVLLFIGGGASLVILSMYDVILSRALKMDISLGKVLRVSYIINALNAIVG
                     FGGFIGAGVRAMVYKNYTHDKKKLVHFISLILISMLTGLSLLSLLIVFHVFDASLILD
                     KITWVRWVLYVVSFFLPLFIIYSMVRPPDKNNRFVGLYCTLVSCVEWLAAAVVLYFCG
                     VIVDAHVSFMSFIAIFIIAALSGLVSFIPGGFGAFDLVVLLGFKTLGVPEEKVLLMLL
                     LYRFAYYFVPVIIALILSSFEFGTSAKKYIEGSKYFIPAKDVTSFLMSYQKDIIAKIP
                     SLSLAILVFFTSMIFFVNNLTIVYDALYDGNHLTYYILLAIHTSACLLLLLNVVGIYK
                     QSRRAIIFAMISILLITVATFFTYASYILITWLAIIFVLLIVAFRRARRLKRPVRMRN
                     IVAMLLFSLFILYVNHIFIAGTLYALDIYTIEMHTSVLRYYFWLTILIIAIIIGMIAW
                     LFDYQFSKVRISSKIEDCEEIINQYGGNYLSHLIYSGDKQFFTNENKTAFLMYRYKAS
                     SLVVLGDPLGDENAFDELLEAFYNYAEYLGYDVIFYQVTDQHMPLYHNFGNQFFKLGE
                     EAIIDLTQFSTSGKKRRGFRATLNKFDELNISFEIIEPPFSTEFINELQHVSDLWLDN
                     RQEMHFSVGQFNEEYLSKAPIGVMRNEENEVIAFCSLMPTYFNDAISVDLIRWLPELD
                     LPLMDGLYLHMLLWSKEQGYTKFNMGMATLSNVGQLHYSYLRERLAGRVFEHFNGLYR
                     FQGLRRYKSKYNPNWEPRFLVYRKDNSLWESLSKVMRVIRHK"
BASE COUNT         4943 a         2831 c         2251 g         5430 t
ORIGIN      
        1 taaacgcttt aattgcgtat acttttatag taatatttag atttttgcat acaatttcaa
       61 aaaaagtaat atgaacgttt gggtttgctc atattacttt tgtatcattt ctattcaatt
      121 ttataattca ccgtttttca ctttttcaaa cagtattcgc ctaatttttt taaatcaagt
      181 aaacttaatt attcaatgtt tgttggatag attgtaaata tctaatgatt tcctcacgcg
      241 tgttagattt aaatcgctta acgatttcgc taccaatgac gatgccatct gcaacctctt
      301 ttatatctgc aacatgttgt ggtgttctta taccaaatcc tgcgacaact ggcacattgg
      361 ctatcgcttt aattgactca atttttcgtt ttaattctgg atgaaacgca ccgttttgtc
      421 ctgttgtcgc attcatcgtc acagtataaa taaagccttc cgcatgagat acgatatctt
      481 ttatacgttt gtcatcagta gtcatcgcaa ctaacgatat gattttgacg ccatagtgac
      541 taaattgttg ttttaaacgc tgcgataatt catatggtaa atcaggaata attaagccgt
      601 agacaccagt atctcgacat ttttcaaaaa acgcttgttc tccataatga caaataatat
      661 tataatacgt cattaataca tagttacact taatttgatc accatgtttt tctaattgat
      721 tgaaaatata atctatcgtg atgccttgtt taatcgcttg ttgacctgct tccatgataa
      781 ctggaccatc agcaaccgga tcagagaaag gtacaccaat ttcaattata tctgcaccat
      841 tttcactcaa caatgttgca ttttcaatca aatctttatt gcccataata taaggtataa
      901 ataatttagt catttgcaag acctcgctct gccatatatt gtctaattgt ttccatatct
      961 ttatcgccac gtccagaaat agttactaca ataatatctt ctttcgacat cgtaggcgct
     1021 agtctttcaa cataactcag tgcatgtgca ctttcaattg caggtataat accttcatgt
     1081 tttgtaaagt tgattaaagc attcattgct tgtgtatcac ttgcattttc aaaagttact
     1141 ctaccaatgt cgtggtaata agaatgttca ggcccaatac caggataatc aagtcctgct
     1201 gaaatagaat gtgctagttg cacttgccca ccttcatctt gaattaaata cattttagta
     1261 ccatgtaata cgccaggtga tcctttgcca attgcaagtg catgtttatc agtatcctcg
     1321 ccttgacctg cggcttcaac accgtataat gcaacatcat ctttaataaa tggataaaat
     1381 gtaccgattg catttgagcc accaccgata catgctacaa ttgcatccgg aagtcgacct
     1441 tctttcttca atatctgtga ttttatttct ttaccaatga cactctgaaa atctctaaca
     1501 atcgttggga atgggtctgg acctaatgca gaacctaata aataatgtgt atcatctaca
     1561 tgacttaccc aatattgcaa tgctttatta actgcatccg ataaagtccc ttgaccttct
     1621 tcaactgcca caacctttgc accaagtaat tccattctaa atacattaag ttgttgtctt
     1681 ttaatatctt cacttcccat aaagacaaca agttccatat caaataatgc agcaaccgta
     1741 gcactagcta caccatgttg acccgcacca gtttcagcaa caagcttctt cttgcccatt
     1801 cttttagcaa gcaacgcttg acctaacgca ttattaattt tatgggcgcc tgtatgattt
     1861 agatcctctc gtttcaaata tattttagcg ccacctaggc tttcagtata tgatgcagca
     1921 tatgtaagtg gtgtcgcgcg tcctacatac tctgataaat agtattccag ttctctttga
     1981 aactctgggt ctgcttttgc ctctttataa gcttttttca actcaataat tgctggcatt
     2041 aatgtttctg gaacatattg ccctccatat tcaccaaaga aacctaattc atctgcttct
     2101 gtttgtattt gtttattcat tgtctctatc tcctttcaca atatttacaa ttgctgtcat
     2161 tttttctata tcttttcgcc catttacttc tatacctgat gcaagatcat aaccttgatg
     2221 tgataattta agttgattaa ctgtttgaat attttcagag ttaatgcctc ctgctatcaa
     2281 ataaggtatg tcttttatgt gcttcaaaat agtccagtca tatgtttgac cggtaccacc
     2341 atacgacact gagggtgtgt cgataataaa taaatctacg aaccctttat atttatttat
     2401 gttttggatt atgttttcat ctgcagctaa agctttagtg attttaatgc ttgaatattt
     2461 ctttttaatt tcctgtataa aatcaataga ttctgtgccg tgtaactgta ttgtgttaat
     2521 tgacgtattg cttaatacgt gttcaattgt tgttaaatca ggatttacca tgacacatac
     2581 tttatcgata tgatttggaa cagcagacgc taactttttt atttgggtaa ttgtttgatg
     2641 ccttttactt ttttcataat ggatgaaacc tatcgcatca ataggtaatt gactggccgc
     2701 tgtaacatcc tttattgatg taaagccaca aaatttcaat ttcatcatga cttcaccttt
     2761 tgcattttca gttgtggtaa aaattcagat agattgtcac aacgcataag cgcctcacct
     2821 attagtaagc catcgatacc actatgcaag atttttctta catcagatgc atcgtgaata
     2881 ccactttcag aaatataatg atgattgggt tttttatttt ctaaaatagt atttgtatgt
     2941 tccacatttg tgacaaatcg ttttaagtcc ctgttattta caccaatcaa tttagcatta
     3001 accttatagg cacgttctaa ttcatggcga tcatgtactt caattaacac ttctagattt
     3061 tgcgatatag cgtagttata taaatctttc aattgtttat ccgataagat gttaacgatc
     3121 aataaaatca tagatgcacc agcttgttta gcaacatcaa tttgaagcgg gtctataata
     3181 aagtctttgc ataatacggg taatgttgtt tttgttgtca atgcttgtaa tctttcaaaa
     3241 ctaccaccaa agtacttttc atcagttaaa atggacacgg catttgcacc gtacttttca
     3301 taatctgaga tttgttgcga taaatctcgt tcaggtaagt cattaactgt aggactcttc
     3361 gatttaattt ctgcaataat tgctagcttt ggttctttct caattgcgtt tataaaagat
     3421 tttttattct gaatcttcac acttttcaag gtattaagtt tgtcttgata atagccattt
     3481 tgtaaaagtg actgtttata tttaacaatt tctgctaaaa tcgtcatatt attcacctct
     3541 catttgatgg tattttttca atgcttcacc attatcaatc aatgtagttg caagttctat
     3601 gccttctgcg atggtatcca ctttctctgc aacataaagg cttaaacccg catttagtaa
     3661 gacaacatca cgtcgacttg actgatcttt accattcaag atattaaggg agattgctaa
     3721 attttcttca ggtgaaccgc ctttaaaatc actattcggc gcatgtttca aaccataatc
     3781 agtcgcattt aatgtgtaat ttttgatttc tccatcttca gtcaattcat atatcaaatt
     3841 atcaccagat agtgttgctt catccatacc atttgcacca tgtaaaacga ttgcacgttt
     3901 tctacctaaa tctttaatcg ttttagcaac taactttaac tttgtaggat caaagacgcc
     3961 taccatttga tacgttaagt gatatggatt aattaatgga cccacaaggt ttaatattgt
     4021 aggctttcca atcatttttc taactggttg catatacttc atgattggat atgattcagt
     4081 tgcaccaatg aatacaaggt ctttttcatt taattggtta ggtgtatcat caacagttgt
     4141 tgtttgtatg ttcatttgat ttaacaaatc cgtactacct gaatttgagg taatactttt
     4201 attaccatgt tttataactt ttacgccagc acttgctaca acaaaagcaa cagtcgttga
     4261 aatgttgaaa ctatttgact tgtcaccacc tgtgccgcac acacacatag ccccttcata
     4321 acatggttga tgtggataca ttgtattaat taagctacgt acaatatatg ttaattcttg
     4381 ttgttggatt tctcgctccg aataggaact aagtaattca tatttaatat cagttccaat
     4441 actaggagaa ataagtatat cgattagctc tttaatgtcg ctttcaagta atatagtttc
     4501 agtttttatt cttgttagta atgtcatggt tttcatcctt ccttcactag attaatgaaa
     4561 tttgtaatta ttttgacacc atagtctgta gcaaatgatt caggatggta ctgaataccg
     4621 taatgcggtc tttctttatg ctcaaatgac tgtatacaat cttcggtacg tccagtaatt
     4681 ttcaattctt ctggaaaatt gtcagggtta cttattaatg aatgatatct cataattgaa
     4741 aactgttctg gtatatcttg atataacaga tgttgatgat gcgatataac ctttagtgta
     4801 tcaacttttc cgtgcataac cttgtcgcct ttaatgactt ctccaccgta ataacaagtc
     4861 agtgcctgag cacctaggca aatacctaaa atgggtttgt gttgataggt tgatatgatt
     4921 ttcattaact gttgatcgtc taatggatgc cctggaccag gagatataat aacagcgtcc
     4981 accgattgat tcagcacatt atcatcatca ggatattgaa caatgacgtc agtatgttga
     5041 gcaacaatat ccactaggtt atatgtaaag gaatcataat tatctacaac taagatcatg
     5101 gcctcacctc caataagctt ttagctttca atttcgtttc attcagttct ttttcaggaa
     5161 tagaatcata tacaacgcca caaccagctt ctacgttgat atactgctca tctatcatca
     5221 tcgttcgaat tgctaaagca aaatctaagt tatgattaca atttatgtat ccaacaccac
     5281 cactataaac gcctcgttta tgtggatatt gttcatatat tctttcaatt gcacgtaatt
     5341 ttggtgcacc tgaaacggta cctgttggta ataaattcgc aataactgtc attggtgata
     5401 aattttgatt tattttacct gtgatttcac ttacgatatg cataacatgt tcatattttt
     5461 caataaccat taatttagta atttttgagg taccgatttt acttactcta tgaatatcat
     5521 tacgtcctaa atcaactagc atacgatgtt cgctgcattc ttttggatca ttaagtagtt
     5581 gtttcatatt ctcattatct atttgtgtcg tctcaccacg ttgaatcgta cctgcaatag
     5641 gattagttgt tacaatttga tctttgacac ttacgaaact ttcgggagaa ctaccgacaa
     5701 tatatggttg atcgatatta agataataca tatatggact tgggttttgt cgttttaaat
     5761 tttgatacag ttgaaacgaa agttgattta aatgctgact agcatgatgc gcatatttgt
     5821 aaattcttga tggcacaact tggaacatat ccccttctgt tattttctct ttgaaatact
     5881 gaatcatttc gataaatctt tcttcagaaa tatttgattg aatttcttta gttttaaaat
     5941 caaaatcttg tgtaggcatg aatggttgga ttttagttaa gtcttcgata gacttattaa
     6001 ctcgattctc aagatctgat tttgttgaat ttgaaaattg attcgtcgcg atgatatata
     6061 actcatcttt gtaatggtca aatacataaa cttgttcaac catatataac cttacatcgt
     6121 gctgcttgtg atcttctaat tgtattgatt gcaatttagg aaattcatgt cttactaaat
     6181 caaagctaca cgtcccaaca tatccagata taaaccgtaa tgacttaagt tgctcatctt
     6241 gaatattatg gtagtcttca tttattttag tcgttaaata atggtacggt ctttcaatga
     6301 tttgatacga ttcttttaaa gtgcttactg ataatacatc attatctaaa gttaaagtgc
     6361 cataaatatc aaaaataaca actgaatagc gacctttagt ttgttgttga tttgtacttt
     6421 ccaaaatgat cttcttggaa tgaagttgtg ctaaaacttc gggcgttaca tttgctttta
     6481 tttttttata aaatatatcc attctatacg ctcctttcaa aaaagcgcca gcatccttat
     6541 caatataaag gacgctgacg cgtggtacca cctaaattaa ccgaatattc aaaatattca
     6601 tagttcactc tttactatat ataatttttc tgttgcagac aaaacccaat ttcattaatc
     6661 tcggtgttag ttcacatcat ccactaactt tctgttgcta cctttaatta actactacat
     6721 tttgtctaaa taaaaagaca ccacataacg catatacctg taaggacgcg ttaccgtggt
     6781 gccaccttag ttaacataat tcatgttcac tttcaaaact cattcaattg ttttccaaag
     6841 tccaattcac aagatatggc gtgctaattc ccatcaccca ttagctttct atttgctacg
     6901 tttcatatct gctactattt ctttagaaaa ttctaattat ttttatagtt cattatatta
     6961 cataggtcgt tcacatttgt caacactaat tgtttactca attggtgata agcaatatgc
     7021 atatagtaat ttctctgtcg ctttaataga atcaatatgt gttcgttcca ttgcatgaga
     7081 tgattcaatg ccagcgccaa ataaaccatg tctgatatcc gcaccagcat gtaaagctgc
     7141 tgaagcatct gaaccataat atggatatat gtctacttta tatggaatgt tatttatttt
     7201 gcaaagatta actaggtgcg atttcaattg cttatgatat ggacctgaag catctttggc
     7261 acaaatagaa actgtatatt catccgatgc ttgaccgtct cccaacgcgc ccatatctaa
     7321 tgcaatatat tctttgattt tcgaatcaat tgatgcattt gcaccgtaac ctatttcttc
     7381 gttattagaa atataaaatt gcgttgtatg tggtaatatt atttgctctt cttttaattt
     7441 ctttagtaat tgtagtatca tcgctacgct agctttatca tctaaatgac gagatttaat
     7501 aaaacctgat gacgtgataa ctgtacgtgg atcaaagcta acaaaatcac ctactgaaat
     7561 acctaaactc tttgtatctt cttctgatgt agttacttca tcaattctta tttccatatg
     7621 cttttgatct ctaggtattt catgattatt tctatataca tgaacacttg tttcatgcag
     7681 acaaattgtt cctgtatata tttgaccagc atcagtttta atttggcaat attcaccctc
     7741 aatcgcgtta tacgtgaatc ctccaattaa ttctattgct aagcgaccat cttctttaat
     7801 ttctttaacc attgcaccta aagtatcaac atgagcagta atacagcgtt gtacttcatc
     7861 atttttgcct ggcactgtta ttaataacgc acctttattt gttagtgttg tttgataacc
     7921 caagtctttt gcatattttt cgacaaattg aattgcttct tctgtgtctc ctgaaggact
     7981 attaatacct gttaatgttt taatcatttc taatattggt tccattgatt tacccctccg
     8041 ttgccattgt gttactatac tctttattgt agatgattta tctccgttta tctattaatg
     8101 ctattatcga tttttattta ctattgcact gtgtcaacag ctcatttaca atgttattat
     8161 tctgaaaatt tcgaaataag gtgatttata tgacaacagt tttatttgtt ggacttggat
     8221 taattggtgg aagtcttgct agcaatataa aataccataa ccctaatact aatattattg
     8281 catacgatgc agatacttct cagttagata aagctaaatc aatcggcatt attaatgaaa
     8341 aatgtttaaa ttatagtgaa gctattaaaa aagccgatgt aattatttat gcaacacctg
     8401 ttgctatcac aaataaatat cttagcgagc ttatagatat gccaactaaa cctggtgtta
     8461 ttgtttctga tactggtagt actaaagcaa tgatacagca acacgaatcc aatttattaa
     8521 agcataatat tcatttagtc agtggtcatc caatggctgg tagtcataaa tctggtgtac
     8581 taaatgctaa aaagcactta tttgaaaacg cttattatat tttagtctat aatgagccaa
     8641 gaaatgagca agcagcaaac acgttaaaag aactgttatc acctactctt gctaaattta
     8701 ttgtaactac tgctgaagaa cacgactacg taacaagcgt cgtaagtcat ttacctcata
     8761 tcgttgcatc tagtttagtt catgttagtc aaaagaacgg tcaagaacat catttagtta
     8821 ataaacttgc agctggtggt tttcgtgata tcactcgtat agctagtagt aatgcacaaa
     8881 tgtggaaaga tatcaccttg agtaataaaa cgtatatttt agaaatgatt cgacagctaa
     8941 aaagtcagtt tcaagattta gaaagactaa ttgaaagcaa tgattctgaa aaattgttat
     9001 cattttttgc ccaagctaaa tcgtatcgcg acgcactacc cgctaaacaa ctaggtggac
     9061 taaatactgc gtatgatcta tatgtagata ttccggatga atcaggtatg ataagtaaag
     9121 tgacttatat catgagttta cataacatat ctataagcaa cttaagaatc ttagaagtac
     9181 gcgaagatat atacggtgct ttaaaaatta gtttcaaaaa tcctactgac cgagaacgcg
     9241 gtatgcaagc attgagtgat tttgattgtt atatccaata attattaagt ctcttttgat
     9301 aaattcccat gtattcttag atattattgt gaaatctatt gtgatatatg aaatatttat
     9361 ctttagagac ttattttata cgaatcacag ttatatattc cttaaaccgt atagtcatta
     9421 cattttatgt ccagccatta aacctaatct accatgtttt gtacctgctt ctgtaaacga
     9481 tactgctttg gacacaatac ctttgccgta tttcaaatgt aattggtcta tcgttttagc
     9541 taggcattcg tcacgtttgc gttggtattc atcttcaaac agacttaact gtcgctcatc
     9601 ctcattaata aattgactca aagatatact tagcgtacga tatagtgctt gtttattaca
     9661 taatttatct gcaaaatgca ttactacttt ataaatatct ttttctaagt ttgttggatc
     9721 tttcaaagta tattgcttat gtacaccgcc ttcatcacta tagccaaagg caaaatgtat
     9781 cgttcttgcc actttttttc ttgctcgaac tctgctagca acatcttcaa ttaactcttg
     9841 cattactact tttgcttcat caaaatgata atctctcatt aatatttgac ttttgcatat
     9901 cgatggattg ctgatcttgt gcttttcacg tactttactc tgatctatcc cattcgcatg
     9961 tagatgcata tcaacaccta aaataccgaa ctcttttttt aaaaatttat atggatattt
    10021 cgctaaatct cctatagtaa aaattcctct tttattcaat ttggcttctg ttcgacgatt
    10081 aataccccaa aaatctcgca agggctgaat tggccataat ttcgttggta catcttgata
    10141 tcgccattca gctataccat tttgactatg cttcgcttca acatccatag caattttact
    10201 taataacata ttagaaccaa tgcccacagt acaataaatg cctgtttctt cataaatttc
    10261 acgtttaagt ctttcgcaaa atgcatgtac tgtagaacta aatctatgat agctatcagt
    10321 aacatccata aaaaattcgt caatactata ttggtgtaaa tcttcaggtg gaatataacg
    10381 caatgcaatc ttagaaatag caactgaaac attaagatat ttacgcatac ttggattgat
    10441 aatgtaaata tcatttctat gtggtatttc aaacaatcgc gaccctgtct tgatgcctaa
    10501 ttcttttaat ttaggtgtcg cagccaatat tacagaaccc tgacgcttag tatctgcaac
    10561 aacagctaac tttgtttcta atggatctag ccccttttca atacaagaaa cactcgcaaa
    10621 aaaacttttt tggtcaatac atagaacatc cctatcttct aataaatgat aattatacac
    10681 tgttattcct cccttacttg aaccttatta tatacgaaca cgtgttctat ttcaactatt
    10741 ttagaacaaa tgttctgtta ttttgcaaat gaatttttaa gtattacatg atatactcaa
    10801 attaataatg attaggaggt ttggatgatg ccaatcgtca atgtaaaatt attagaaggt
    10861 cgttcggatg aacaattaaa aaatttagtt agcgaagtaa ctgacgccgt agaaaaaaca
    10921 acgggggcaa atagacaagc aattcacgtt gttatagaag aaatgaaacc aaaccattat
    10981 ggtgtggctg gcgtaagaaa gtcagatcaa taattcttca taagatgcat gccaattaat
    11041 tctttgaaaa cgaacaaggc gacttctatc tgagtatgat agaaatcgcc ttgtttattt
    11101 ttaatcttca tctaaaaagt ctttaatagc ttgtttattt gtgtttttat taatctgtaa
    11161 tgcactacca tcagtatttg tattgacatc ttcgtatgag ttcttgattg gcacagtcaa
    11221 tgacttaaca tctttttcac ctcggatgcc aaaactcaaa cctgtttgga aaatccctga
    11281 atcaggaatg tttgtattca catagcctct taaaatacct gcaacttttg gtaatttaac
    11341 aactgttcta aaattaacca tttctttttt caatgtttgc atcacttgtt gctgacgtcg
    11401 cacgcgtccg aagtcacctt cagggtcgtg acggaatctt gcataaccaa gtaattcttt
    11461 accattcaac ctatggttac cctttttcaa agatacacca atatttttcg acatatcctt
    11521 ttcgacatta attggtacac cttctggcat taattcatca atcattttct caaatccagt
    11581 aaaatcaact actgcataat attcaggatt aattcctaaa tttttatcaa gtgtttttct
    11641 aagtagctct ggaccaccta aagcgtatgc tgaattaatt ttgtgttttc catatcctgg
    11701 aatatctgca taaatatcac gcatgacaga catcattttc atttttttat tgataaagtc
    11761 atattgaaca accatgatag aatctgttct tgattgtcca ccttgtgctt tatctgcacc
    11821 gagtacaaga atagaaattt taccatcatt ttttactggt ccattaaatt gatgtacttt
    11881 aacatctttc gcatgtttct tggcatattc tacaccgcta ttgtaactat gtacaatata
    11941 tacaactaat gcgataagta aaattacaac aatcagaaga atgataggta atttcctaat
    12001 tttcttcttc ttttttcgct taggcgccga agtgcgatgt tcactttgac gtctatattc
    12061 gttgtcatta gtttctttat ccatatttac ctaccttata tcttcaaaaa tagatttttg
    12121 tgtactataa taatcacata ttaacgttat ataaaaacaa ttcaaaaagc aagtgatttc
    12181 ttaataaatc gtactaaggt ctaatgaaag gatgtcaaaa atgaatatta atacagctta
    12241 ttttgccgga ggttgctttt ggtgtatgac gaaaccattt gacacctttg acggcataga
    12301 aaaagtaact tctggatata tgggcggaca tattgaaaat cctacttacg aacaagtaaa
    12361 atcaggtacg agtggtcatt tagaaactgt tgaaattcaa tatgatgttg cattattctc
    12421 atacaataag ttattagaaa tatttttctc agtcattgac ccattagata caggtggtca
    12481 atatcaagac cgtggtcctc aatatcaaac agctattttc tacactaatg atcatcaaaa
    12541 agaactcgct gagacatata tcgagcagct taaaaatacg attaatgctg ataaggcaat
    12601 tgcaacaaaa atattaccag cgtcacaatt ttacaaagcc gaagactatc accaagattt
    12661 ttataagaaa aatccagagc gctatgcaga agaacaaaaa atacgccaag aatacaaaaa
    12721 taagcaataa catttatact tgatgaaact gattgggctg ataataaaag ttaggcgact
    12781 taacttaagc tcatttcaat attaaaatac ggtacagata ggaaacttaa atattcttat
    12841 ctgtaccgtt ttttataaat cattctttat agaaaccaaa aaatccaagc gcttcaggca
    12901 taactgtata cctcttagca cttggatttt aattatttgt gacgtattac acgcattact
    12961 ttagaaagtg attcccaaag cgaattatct ttacgataaa ctaaaaagcg tggttcccaa
    13021 ttcggattat atttagattt ataacgacgt aatccttgga aacgatatag accgttgaaa
    13081 tgttcaaaga cacggcctgc aagtcgttct cttaaatatg aataatgcaa ttgaccaacg
    13141 ttcgataacg ttgccatacc catattaaat tttgtataac cttgttcttt actccaaagt
    13201 aacatgtgca agtataatcc atccattaat ggtaaatcta actctggcaa ccatctaatt
    13261 aaatcgactg aaatggcatc attaaagtat gttggcatta aactacagaa tgcaattact
    13321 tcattttctt catttcgcat tacaccaatt ggcgctttag ataagtattc ttcattaaat
    13381 tgacccacag agaaatgcat ttcctgacga ttatctagcc ataaatcact tacatgttga
    13441 agctcattta taaattcagt tgaaaacggt ggttcaataa tttcgaatga aatattaagt
    13501 tcatcgaatt tgtttaaagt cgctctaaat ccacggcgtt ttttacctga agttgaaaat
    13561 tgcgttaaat caataattgc ttcttcacct aatttgaaaa attggttacc gaaattatga
    13621 tataaaggca tgtgttgatc tgtaacttga tagaatataa catcatagcc taaatactca
    13681 gcgtaattat agaatgcttc taacaattca tcaaaggcat tttcatcacc taacggatct
    13741 ccaagaacca ctaatgaact tgctttataa cgatacatta aaaatgctgt tttattttca
    13801 ttagtgaaaa actgcttgtc accactatat atcaagtgac tcaaataatt accgccgtac
    13861 tgattaataa tctcctcgca atcttcaatt ttagaagaaa tacgtacttt gctaaattga
    13921 taatcaaaca accatgcaat catacctatg atgatagcga tgattaaaat cgtaagccag
    13981 aagtaatagc gcaatacaga tgtatgcatt tcaatcgtat aaatatctaa tgcatataac
    14041 gttccagcaa taaatatatg gttaacatat aaaataaata aactgaataa aagcattgca
    14101 actatatttc tcattcttac tgggcgtttc aacctacgtg ctctacggaa agctacaata
    14161 agcagaacaa aaataatagc taaccatgtt attaaaatat atgaagcgta agtgaagaat
    14221 gtcgccactg tgattaataa aattgaaatc atagcaaaga taatggcacg tctactttgc
    14281 ttataaatac caactacatt cagtaaaagt aataaacaag cactagtatg aattgccaat
    14341 agaatataat acgttaagtg atttccatca tataaagcat cgtaaacaat cgttaagtta
    14401 tttacaaaaa agatcatact tgtaaagaat actaaaattg ctaatgataa tgatggaatt
    14461 ttagcaataa tatccttttg ataagacatt aaaaatgacg taacatcttt agcaggaata
    14521 aagtatttag atccctcaat gtacttctta gctgatgtac caaattcaaa tgatgataaa
    14581 attaatgcaa taattaccgg tacaaaatag tacgcaaaac gatatagaag tagcattaat
    14641 aatacttttt cctcagggac acctaaagtt ttaaatccta gtaatacaac taaatcgaaa
    14701 gcgccgaaac caccaggaat aaagctgact aaacctgata atgcagcaat gataaatatt
    14761 gcaataaagg acatgaatga tacatgagcg tcaacaatta caccacagaa atataataca
    14821 actgcagctg ctaaccattc aacacacgac actaaagtgc agtacaatcc tacaaaacga
    14881 ttgtttttat cgggtggtct aaccattgaa taaataatga ataatggtaa gaaaaatgac
    14941 actacatata atacccatct tacccatgta atcttatcta agattaaaga tgcatcgaaa
    15001 acatggaata caattagcaa tgataataag cttaaacctg tcaacattga aataagtatt
    15061 aaggatataa agtgaactaa tttcttttta tcatgcgtat agtttttata aaccattgct
    15121 ctaacgcctg caccaataaa gccaccgaaa cctacaatcg cattcaatgc attgatgata
    15181 taacttactc ttaaaacttt gcctaaggat atatccattt ttaaagctct agataaaatc
    15241 acatcataca ttgatagaat aacaagcgat gccccaccac caataaatag taacactaag
    15301 gacatacggt taatcttact aaattcaacc aacgtatctt taaagttaat accagataac
    15361 tcccgataca atgtgattgc tacaaaaata aataaagctg tagcaaacgt aatttttaag
    15421 attgaaaata ttttgttttt aacttcctga ttcat
//
LOCUS       DAHHSC010000045        15439 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.045, whole genome shotgun sequence.
ACCESSION   DAHHSC010000045 DAHHSC010000000
VERSION     DAHHSC010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 15439)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15439)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15439
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.045"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            8..1867
                     /locus_tag="NBI57_001079"
     CDS             8..1867
                     /locus_tag="NBI57_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase domain-containing protein"
                     /protein_id="HCD1822039.1"
                     /translation="MPKNKILIYLLSTTLVLPTLVSPTAYADTPQKDTTAKTTSHDSK
                     KSNDDETSKDTTSKDTDKADNNNTSNQDNNDKKFKTIDDSTSDSNNIIDFIYKNLPQT
                     NINQLLTKNKYDDNYSLTTLIQNLFNLNSDISDYEQPRNGEKSTNDSNKNSDNSIKND
                     TDTQSSKQDKADNQKAPKSNNTKPSTSNKQPNSPKPTQPNQSNSQPASDDKANQKSSS
                     KDNQSMSDSALDSILDQYSEDAKKTQKDYASQSKKDKNEKSNTKNPQLPTQDELKHKS
                     KPAQSFNNDVNQKDTRATSLFETDPSISNNDDSGQFNVVDSKDTRQFVKSIAKDAHRI
                     GQDNDIYASVMIAQAILESDSGRSALAKSPNHNLFGIKGAFEGNSVPFNTLEADGNKL
                     YSINAGFRKYPSTKESLKDYSDLIKNGIDGNRTIYKPTWKSEADSYKDATSHLSKTYA
                     TDPNYAKKLNSIIKHYQLTQFDDERMPDLDKYERSIKDYDDSSDEFKPFREVSDSMPY
                     PHGQCTWYVYNRMKQFGTSISGDLGDAHNWNNRAQYRDYQVSHTPKRHAAVVFEAGQF
                     GADQHYGHVAFVEKVNSDGSIVISESNVKGLGIISHRTINAAAAEELSYITGK"
     gene            complement(2132..2692)
                     /locus_tag="NBI57_001080"
     CDS             complement(2132..2692)
                     /locus_tag="NBI57_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HCD1822040.1"
                     /translation="MSRKTALLVLDMQEGIASSVPRIKNIIKANQRAIEAARQHRIPV
                     IFIRLVLDKHFNDVSSSNKVFSTIKAQGYAITEADASTRILEDLAPLEDEPIISKRRF
                     SAFTGSYLEVYLRANDINHLVLTGVSTSGAVLSTALESVDKDYYITVLEDAVGDRSDD
                     KHDFIIEQILSRSCDIESVESWKSSL"
     gene            complement(2861..4768)
                     /gene="sasF"
                     /locus_tag="NBI57_001081"
     CDS             complement(2861..4768)
                     /gene="sasF"
                     /locus_tag="NBI57_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell-wall-anchored protein SasF"
                     /protein_id="HCD1822041.1"
                     /translation="MAKYRGKPFQLYVKLSCSTMMASSIILTNILPYDAQAASEKDTE
                     ISKEILSKQDLLDKVDKAIRQIEQLKQLSASSKAHYKAQLNEAKTASQIDEIIKRANE
                     LDSKENKSSHTEMNGQSDIDSKLDQLLKDLNEVSSNVDRGQQSGEDDLNAMKNDMSQT
                     ATTKYGEKDDKNDEAMVNKALEDLDHLNQQIHKSKDALKDASKDPAVSTTDSNHEVAK
                     TPNNDGSGHVVLNKFLSNEENQSHSNQLTDKLQGSDKINHAMIEKLAKSNASTQHYTY
                     HKLNTLQSLDQRIANTQLPKNQKSDLMSEVNKTKERIKSQRNIILEELARTDDKKYAT
                     QSILESIFNKDEADKILKDIRVDGKTDQQIADQITRHIDQLSLTTSDDLLTSLIDQSQ
                     DKSLLISQILQTKLGKAEADKLAKDWTNKGLSNRQIVDQLKKHFASTGDTSSDDILKA
                     ILNNAKDKKQAIETILATRIERQKAKLLADLITKIETDQNKIFNLVKSALNGKADDLL
                     NLQKRLNQTKKDIDYILSPIVNRPSLLDRLNKNGKTTDLNKLANLMNQGSNLLDSIPD
                     IPTPKPEKTLTLGKGNGLLSGLLNADGNVSLPKAGETIKEHWLPISVIVGAMGVLMIW
                     LSRRNKLKNKA"
     gene            complement(5009..6367)
                     /gene="gtfB"
                     /locus_tag="NBI57_001082"
     CDS             complement(5009..6367)
                     /gene="gtfB"
                     /locus_tag="NBI57_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system glycosylation chaperone
                     GtfB"
                     /protein_id="HCD1822042.1"
                     /translation="MIKLFDYFNDHSRKLYESFKASKLEEDLTIVLNDNGFLPDDVIS
                     PYQFFADNHNTENMKPRFFNQVTVPAFWEIKGNNNSATINDMGRLRGKIFYQSGERPR
                     IVSRVEWFDDQQRVRFVDYYSKNGIKFAQTVYDLNRKAILKKYMTAEGKEVIYENFVT
                     SDVILDWQGKSYFFPSKLAFVLFFIKQLEITEHHFVINSLALPFSVLYNLPSKGSDVL
                     VWQEHCNGNVPGNMQLMCKGDMKRHCNIIIPDKNEYETMLNIADAKVQSRILQGGYLY
                     NYRSRNRYTKEIVILTNSDQLRNIKVLVETLPDFNFHIAAITEMSDKLMQLDQYANVH
                     LYPSINIDRVNELYQLCDIYLDINEGNEILNAVEQAFDYELLILGYRQTAHHAKVTLS
                     EHLFEHNDEITMESKDQLIQMLESLKDQQQFRDALLAQKAHAHEISREQFEQVFKQAL
                     ES"
     gene            complement(6360..7868)
                     /gene="gtfA"
                     /locus_tag="NBI57_001083"
     CDS             complement(6360..7868)
                     /gene="gtfA"
                     /locus_tag="NBI57_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system glycosyltransferase GtfA"
                     /protein_id="HCD1822043.1"
                     /translation="MTIYNINFGIGWASSGVEYAQAYRAKLLRRTNQDTKFVFLDFIQ
                     SENIQTLTQNIGFKDQEVIWLYQYFSDISIAPTTYTLDDIERELGHEISFRERNGKIV
                     RLYFNGKSSFVTCYLQNEQKDVVDRAEFVINSMLVRKDFYSYTRIFSEYYAPADNKAK
                     LYMRQFYNEDGSIAYNEYIDGDSSIYVFEDAVLYNKTEFIAYFLQRLNLTRDDIVILD
                     RASDIGQAVLQHKGDSKVGVVIHADHYSNNMMSEQHILWNNYYEYQFSKAKYIDFFIT
                     ATDIQNHMVCRQFEQYQGYRPRVYTIPVGSIDALSYPKLSRKPYAMISASRLANEKHI
                     DWLVKAVIVAKRQVPELTFDIYGEGSEKTRLRKIIDTHRAQDYIRLLGHVKLDEIYND
                     YELFLSASTSEGFGLTLMEAVGSGLGMIGLNVNYGNPTFIRDGENGYLVPFDTDEDSV
                     DDVIAKLAHAIVMYFNNGPQAPHDISYEVAQQFMTQDIILKWETLVQEVLHD"
     gene            complement(7886..10276)
                     /gene="secA2"
                     /locus_tag="NBI57_001084"
     CDS             complement(7886..10276)
                     /gene="secA2"
                     /locus_tag="NBI57_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system translocase SecA2"
                     /protein_id="HCD1822044.1"
                     /translation="MKHKLDVTINELRLKSIRKIVKRINTWSDEVKSYSDDALKQKTI
                     EFKERLASGVDTLDTLLPEAYAVAREASWRVLGMYPKEVQLIGAIVLHEGNIAEMQTG
                     EGKTLTATMPLYLNALSGKGTYLITTNDYLAKRDFEEMQPLYEWLGLTASLGFVDIVD
                     YEYQKGEKRNIYEHDIIYTTNGRLGFDYLIDNLADSAEGKFLPQLNYGIIDEVDSIIL
                     DAAQTPLVISGAPRLQSNLFHIVKEFVDTLIEDVHFKMKKTKKEIWLLNQGIEAAQSY
                     FNVEDLYSEQAMVLVRNINLALRAQYLFESNVDYFVYNGDIVLIDRITGRMLPGTKLQ
                     AGLHQAIEAKEGMEVSTDKSVMATITFQNLFKLFESFSGMTATGKLGESEFFDLYSKI
                     VVQAPTDKAIQRIDEPDKVFRSVDEKNIAMIHDIVELHETGRPVLLITRTAEAAEYFS
                     KVLFQMDIPNNLLIAQNVAKEAQMIAEAGQIGSMTVATSMAGRGTDIKLGEGVEALGG
                     LAVIIHEHMENSRVDRQLRGRSGRQGDPGSSCIYISLDDYLVKRWSDSNLAENNQLYS
                     LDAQRLSQSNLFNRKVKQIVVKAQRISEEQGVKAREMANEFEKSISIQRDLVYEERNR
                     VLEIDDAENRDFKALAKDVFEMFVNEEKVLTKSRVVEYIYQNLSFQFNKDVACVNFKD
                     KQAVVTFLLEQFEKQLALNRKNMQSAYYYNIFVQKVFLKAIDSCWLEQVDYLQQLKAS
                     VNQRQNGQRNAIFEYHRVALDSFEVMTRNIKKRMVKNICQSMITFDKEGMPVIHFP"
     gene            complement(10266..11225)
                     /gene="asp3"
                     /locus_tag="NBI57_001085"
     CDS             complement(10266..11225)
                     /gene="asp3"
                     /locus_tag="NBI57_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system protein Asp3"
                     /protein_id="HCD1822045.1"
                     /translation="MLKNKTFKVTWNYISQTTFMYGSKVSFSNGEVTFINPLMPSGLP
                     IHEWLMLKQFSKYKSAPSLPILRRGQHYKLHFDFDATPAGSVYFIIIFYNKNGTKLST
                     EIVKSNSITIQYPDEAYAYKIKMMNAASTSLIFRCLTITEMTHQDDLEYKSMRVTKID
                     DNQYGNDRINVIIAEPSDTYPTISNDFLKLFGHVWLVERWMDDDIKENIKQLKDDLQS
                     QDTLTAINLISYGSKSNVFATYVAQHLDCKVYRTSHEDDDLKEWLTEHVPGNNELKDT
                     NVEFYFKEEQDNHLNYMSRLMNPVRFLDYLDVSKLNGGEVNET"
     gene            complement(11203..12771)
                     /gene="asp2"
                     /locus_tag="NBI57_001086"
     CDS             complement(11203..12771)
                     /gene="asp2"
                     /locus_tag="NBI57_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system protein Asp2"
                     /protein_id="HCD1822046.1"
                     /translation="MPRKFRVLQIGGDDLEPIFQHKKGVSWDYFDIGLFEFDSGYVEA
                     IEAIVEAEGRFDFIYIQAPYSETLTNLLQMISEPYNTYVDESFWSVEYEQDENVQKYV
                     VQPLHYRNIEERNNKLEAVSFSGQYGDKVSPKLALVHPNFKVDVVYQGNSELTLSGEF
                     GKEFKPIASWQNNLVYDKDKVIQIWPEFDIDGAVELQYTFRLIQTGADGALIEQIILT
                     DDMLDSPLEIPAKPFDAYISVTVKARGNGTVHLGPIHKRWSRLDMGQFLLGGSRFVDS
                     QRQEFIYYFHPGDMKPPLNVYFSGYRTAEGFEGYYMMKRMNAPFLLIGDPRVEGGSFY
                     IGSSEYEQGIINVIDETLEKLNFKSHELILSGLSMGSFGALYYGAQLNPQAIIVGKPL
                     VNIGTIAEHMRLLRPEEFGTALDVLVSNEGDTSQASIKALNQKFWQTFQKKSLSQTVF
                     AIAYMQHDDYDPHAFQELLPVLTAHQARVMNRSIPGRHNDDSPTIASWFVNFYNIILE
                     DKFGRVQHAEKQNI"
     gene            complement(12761..14314)
                     /gene="asp1"
                     /locus_tag="NBI57_001087"
     CDS             complement(12761..14314)
                     /gene="asp1"
                     /locus_tag="NBI57_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system protein Asp1"
                     /protein_id="HCD1822047.1"
                     /translation="MKYFIPAWYDDQRWWQDTTVPYYQLQNKTEFDDMISLMGMHLEN
                     DLDYQLIVLNHAPNLRTFLHRYDLYETKYSSVFDEIQGFSHHAPQAINYHHLKWPDDV
                     EFVYTPYLLKCVTSEQTYTNIYFSQEGYSIWFEEFERDQLQRRYIFDDRGYLSAIRYF
                     DDQGEASYQQYLTINGDCVLHEDLKNGRVTVSKRYQHHYQQTEYNNMAQLIEEKFQAM
                     IAQQIHEDDHVIVASDARHNRQIANHIPAKLLSYSFFKNRNETVSDEEYQSIVKNAHL
                     IVDSVQLERDLISHQEKYQRENTMIRITPFETRQSPNISSQLMETFIGVWIDGMSDAD
                     LQQMMQRLVDYIAQEDYYRLILLSRHQNDIPMWLRECITSVNEEYQAKQNADVNVSAL
                     MTPEDQDDIIAVKTIHAEHDVVEALRTLRLVIDMSKEPDLYLQISAISAGIPQINGQQ
                     TDYVSDYDNGRIINTVDELDDALNYYLFYLKNWNYAYAYSLKLIDAYASKNIINQLDE
                     LIEGENDAT"
     gene            complement(14325..>15439)
                     /gene="secY2"
                     /locus_tag="NBI57_001088"
     CDS             complement(14325..>15439)
                     /gene="secY2"
                     /locus_tag="NBI57_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="accessory Sec system protein translocase subunit
                     SecY2"
                     /protein_id="HCD1822048.1"
                     /translation="SIVSYNDMQVKHESFFKIAISNMGGDVNTLNIFTLGLVPWLTSM
                     IILMLISYRNMDKYMKQTSLEKHYKERILTLILSVIQSYFVIHEYVSKERVHQDNIYL
                     TILILVTGTMLLVWLADKNSRYGIAGPMPIVMVSIIKSMMHQKMEYIDASHIVIALLI
                     ILVIITLFILLFIELVEVRIPYIDLMNVSATNMKSYLSWKVNPAGSITLMMSISAFVF
                     LKSGIHFILSMFNKSISDDMPMLTFDSPVGISVYLVIQMLLGYFLSRFLINTKQKSKD
                     FLKSGNYFSGVKPGKDTERYLNYQARRVCWFGSALVTVIIGIPLYFTLFVPHLSTEIY
                     FSVQLIVLVYISINIAETIRTYLYFDKYKPFLNQYW"
BASE COUNT         4966 a         2973 c         2160 g         5340 t
ORIGIN      
        1 acgaaatatg cctaaaaata aaattttaat ttatttgcta tcaactacgc tcgtattacc
       61 tactttagtt tcacctaccg cttatgctga tacacctcaa aaagatacta cagctaagac
      121 aacatctcat gattcaaaaa aatctaatga cgatgaaact tctaaggata ctacaagtaa
      181 agatactgat aaagcagaca acaataatac aagtaaccaa gacaataacg acaaaaaatt
      241 taaaactata gacgacagca cttcagactc taacaatatc attgatttta tttataagaa
      301 tttaccacaa accaatataa accaattgct aaccaaaaat aaatacgatg ataattactc
      361 attaacaact ttaatccaaa acttattcaa tttaaattcg gatatttctg attacgaaca
      421 acctcgtaat ggcgaaaagt caacaaatga ttcgaataaa aacagtgaca atagcatcaa
      481 aaatgacact gatacgcaat catctaaaca agataaagca gacaatcaaa aagcacctaa
      541 atcaaacaat acaaaaccaa gtacatctaa taagcaacca aattcgccaa agccaacaca
      601 acctaatcaa tcaaatagtc aaccagcaag tgacgataaa gcaaatcaaa aatcttcatc
      661 gaaagataat caatcaatgt cagattcggc tttagactct attttggatc aatacagtga
      721 agatgcaaag aaaacacaaa aagattatgc atctcaatct aaaaaagaca aaaatgaaaa
      781 atctaataca aagaatccac agttaccaac acaagatgaa ttgaaacata aatctaaacc
      841 tgctcaatca ttcaataacg atgttaatca aaaggataca cgtgcaacat cattattcga
      901 aacagatcct agtatatcta acaatgatga tagcggacaa tttaacgttg ttgactcaaa
      961 agatacacgt caatttgtca aatcaattgc taaagatgca catcgcattg gtcaagataa
     1021 cgatatttat gcgtctgtca tgattgccca agcaatctta gaatctgact caggtcgtag
     1081 tgctttagct aagtcaccaa accataattt attcggtatc aaaggtgctt ttgaagggaa
     1141 ttctgttcct tttaacacat tagaagctga tggtaataaa ttgtatagta ttaatgctgg
     1201 attccgaaaa tatccaagca cgaaagaatc actaaaagat tactctgacc ttattaaaaa
     1261 tggtattgat ggcaatcgaa caatttataa accaacatgg aaatcggaag ccgattctta
     1321 taaagatgca acatcacact tatctaaaac atatgctaca gatccaaact atgctaagaa
     1381 attaaacagt attattaaac actatcaatt aactcagttt gacgatgaac gcatgccaga
     1441 tttagataaa tatgaacgtt ctatcaagga ttatgatgat tcatcagatg aattcaaacc
     1501 tttccgcgag gtatctgata gtatgccata tccacatggc caatgtactt ggtacgtata
     1561 taaccgtatg aaacaatttg gtacatctat ctcaggtgat ttaggtgatg cacataattg
     1621 gaataatcga gctcaatacc gtgattatca agtaagtcat acaccaaaac gtcatgctgc
     1681 tgttgtattt gaggctggac aatttggtgc agatcaacat tacggtcatg tagcatttgt
     1741 tgaaaaagtt aacagtgatg gttctatcgt tatttcagaa tccaatgtta aaggattagg
     1801 tatcatttct catagaacta tcaatgcagc tgccgctgaa gaattatcat atattacagg
     1861 taaataagta ttattaaacc cgcaaaattt ataagtataa acaaggagtt cggacttaaa
     1921 catatttctg ttcataagtc cgatttctta ttcaattaaa cccgaggtat tcagttcgaa
     1981 cgcctcgggt cattttatat aaatatatta ttttatgttc aaatgttcct catcatatcc
     2041 gtttcaattg tcatctcaca cattttataa atatgagcaa atgtacttat tttcaaacat
     2101 tactgcctag ctttaattga cgttatatta actataaact acttttccat gactctacgg
     2161 attcaatgtc acatgagcgt gataaaattt gttcaataat aaagtcatgt ttatcatctg
     2221 atctatcacc aacagcatct tctaaaacag taatataata gtctttatct acactttcta
     2281 atgccgtgct caatacagct ccactcgtag agacacccgt taatactaaa tgattaatat
     2341 catttgcacg taaataaact tccaagtaac tacctgtaaa tgcgctaaag cgtcgcttag
     2401 aaataatcgg ctcatcttct agtggtgcta aatcttcaag tattcgtgta gatgcatctg
     2461 cttcagtaat cgcatatcct tgagctttaa ttgttgaaaa cactttatta ctcgaggaga
     2521 catcattaaa atgcttatct aacactaaac gtatgaaaat gactggtatt cgatgttgtc
     2581 ttgctgcttc aattgctctc tgattcgctt taataatatt ttttattcta ggtacactac
     2641 tcgctatacc ttcttgcata tccaaaacta atagcgccgt ttttcgagac atcttcattc
     2701 tcctttactt ctgtagttct aagtcgttaa attcattata acgttaaaat gatggacaat
     2761 ctattcattg cattttgcat atacttcaca ataatttaag ggggaaataa gacgtcttat
     2821 atacttaaaa aaatatatag atgctcttcc cccaatataa ttatgcttta tttttcaact
     2881 tattgcgtcg tgataaccaa atcattagta cacccattgc accaacaatt acagatatcg
     2941 gcaaccaatg ttcttttatc gtttcccccg ctttaggcaa agatacatta ccatcagcat
     3001 ttaataatcc acttaacaat ccattacctt taccaagtgt taacgtcttt tctggctttg
     3061 gtgtgggtat atctggaata ctgtctaata aatttgatcc ttgattcatt aaatttgcta
     3121 acttatttaa atccgttgtt ttcccatttt tattcaatcg atctagtaaa cttggacgat
     3181 ttactattgg tgataaaata tagtctatat cttttttcgt ttgattgagt ctcttttgta
     3241 aattcaataa atcatccgct ttaccattca atgccgattt aactaaatta aaaattttat
     3301 tttgatctgt ttctatttta gtaattaaat ctgccagtaa ttttgccttt tgtctttcta
     3361 tacgtgttgc taaaatcgtt tcaattgctt gctttttatc tttggcatta ttcaaaattg
     3421 cttttaatat atcatctgaa gacgtgtcgc cagttgatgc aaaatgtttc ttcaattggt
     3481 caacgatttg gcgatttgat aatcctttat tcgtccaatc tttagccaat ttatctgctt
     3541 cagcttttcc taatttcgtt tgtaagattt gagaaatcaa tagcgactta tcttgtgatt
     3601 gatcaatcaa tgacgttaat aaatcatcac tcgttgtcag agatagttga tcaatatgac
     3661 gagtaatttg atctgcaatt tgttgatctg ttttaccatc aacacgtata tcttttagaa
     3721 ttttatctgc ctcgtcttta ttaaatatac tttctaaaat gctttgtgta gcatactttt
     3781 tatcatcagt acgtgcaagt tcttccaaaa taatatttcg ttgacttttt atacgctctt
     3841 tcgtcttatt tacttcgctc attaagtctg atttttgatt tttaggaagt tgcgtatttg
     3901 caatacgttg atctaaagat tgtaacgtat tcagtttatg atatgtgtaa tgttgcgttg
     3961 aggcattact tttagccaat ttttcaatca tagcatgatt aattttatcg cttccttgta
     4021 atttatcagt gagttgatta ctatggcttt gattctcttc atttgaaaga aatttattta
     4081 acacaacatg tccagaacca tcattatttg gcgttttagc tacttcatga ttactatctg
     4141 ttgtagacac tgccggatct ttcgatgcat ctttcaatgc atctttcgat ttgtgtattt
     4201 gctgattcaa atggtctagg tcttctaacg ccttatttac cattgcttca tcatttttat
     4261 catctttttc tccatatttt gttgtagccg tttgtgacat atcatttttc attgcattaa
     4321 gatcgtcctc gccactttgt tgacccctat caacatttga agaaacctca tttaaatctt
     4381 taagcaattg atctaattta ctgtctatat cactttgacc gttcatttca gtgtgagaac
     4441 ttttattttc tttgctatcc aactcattag ctcgttttat gatttcatct atttgcgatg
     4501 ctgttttcgc ttcatttagt tgtgctttat aatgtgcttt agatgaagcc gataactgtt
     4561 ttaattgctc aatttgacga attgctttgt caactttgtc taataaatct tgcttagata
     4621 atatctcttt tgaaatttca gtatcctttt cagatgcagc ttgggcatcg tacggcaaga
     4681 tattcgttaa aatgatactt gacgccatca ttgtcgaaca cgataacttt acatataatt
     4741 gaaacggttt ccctcgatat ttagccatca acatactcct ttctcactta cttccttcaa
     4801 agaattacat actattatat acctgtttac aagaaattta cacttatcta tctagttatt
     4861 gttgttagta attatcaact tattacttag cttatattta agtaaacaaa aaagcatgac
     4921 gtaatatcat attgtccatg tcgctaacat catattacgt caaatctttt aaattaaatg
     4981 atgctttatt ttagactgct ttttcttttt agctttcgag cgcctgttta aaaacttgct
     5041 cgaattgttc acgcgagatt tcgtgtgcat gtgctttttg tgctaataaa gcatctcgaa
     5101 actgttgttg atctttcaaa ctttctaaca tttgtattaa ttggtcttta ctttccattg
     5161 ttatctcatc attatgctca aataagtgct ctgataatgt tactttagca tggtgtgcgg
     5221 tttgacgata acctaaaatc aacaactcat agtcaaacgc ttgttccacc gcatttaaaa
     5281 tttcattacc ctcattgata tcaagataaa tatcacataa ctggtatagt tcatttaccc
     5341 tgtcaatatt aatagatggg tataagtgca cattagcata ttgatcaagt tgcattagct
     5401 tatcagacat ctctgtaata gcagcgatgt gaaaattaaa atctggtaaa gtttcaacca
     5461 ataccttgat gttacgaagt tgatccgagt tagttaatat tacaatttct ttagtatatc
     5521 tattacgact acgatagtta tatagatatc cgccttgtaa aatacgagat tgaacctttg
     5581 cgtctgctat attgagcatc gtttcatatt cgtttttatc tggaataata atattacaat
     5641 gtcgtttcat atcaccttta cacatcaatt gcatatttcc cgggacatta ccattacagt
     5701 gttcttgcca taccaaaaca tcactacctt ttgatggcaa attatataac actgaaaatg
     5761 gtagggctag tgagttaata acgaaatgat gttccgtaat ttcaagttgc ttgataaaaa
     5821 ataatacgaa tgcgagcttt gaagggaaaa agtaagactt cccttgccaa tccaatatga
     5881 catcagatgt tacaaaattt tcataaatca cttctttacc ttctgctgtc atatatttct
     5941 tcaagatcgc tttacgattt aaatcgtaaa cagtttgtgc aaatttaata ccattcttag
     6001 aataataatc gacaaatcgg acacgttgtt ggtcatcaaa ccattcgaca cgactaacaa
     6061 ttctagggcg ctctccactt tgataaaata ttttgcctcg tagacgtccc atatcattaa
     6121 ttgtagccga attgttgtta cctttaattt cccaaaaagc tggtacagta acctgattaa
     6181 aaaatcgtgg tttcatattt tctgtattat gattatctgc aaaaaattga tacggtgata
     6241 taacatcgtc cggtaaaaag ccattgtcat tgagtacaat tgttaaatct tcttccaact
     6301 tactggcttt aaaagactca tataactttc gtgaatgatc gttaaagtaa tcaaataatt
     6361 taatcatgta gcacctcttg aactaatgtt tcccatttta aaataatatc ttgagtcata
     6421 aattgctgtg ccacttcata agagatgtca tgtggtgcct ggggaccatt gttaaaatac
     6481 attacaatgg catgagctag ttttgcgata acatcatcca cactatcttc gtcggtatca
     6541 aaaggtacca agtagccatt ttccccatct cgaataaagg ttgggttacc ataattcaca
     6601 tttaatccaa tcatacctag tcctgagcct accgcttcca ttagtgttaa cccaaaacct
     6661 tcgctagttg atgcagaaag aaataactca taatcattat aaatttcatc aagtttaaca
     6721 tgccctagta aacgaatata atcttgtgcg cggtgtgtat caataatttt acgcagtcgc
     6781 gtcttctcgc taccttctcc ataaatatca aatgttaatt ctggcacttg tcgtttagcc
     6841 acgataaccg ctttgacaag ccaatcaata tgtttctcat ttgctaaacg agatgcacta
     6901 atcatcgcat atggctttct tgataattta ggatatgata acgcatcaat gcttcccacc
     6961 ggaatagtat agacacgtgg acgataacct tgatattgct caaattgtcg acaaaccata
     7021 tgattttgaa tatctgttgc tgtaataaag aaatcaatgt atttagcttt tgaaaattga
     7081 tattcataat aattgttcca tagtatatgc tgctcgctca tcatattatt actataatga
     7141 tcagcatgaa tcacaacacc aactttacta tcacctttat gctgcaaaac agcctgacca
     7201 atatcagaag cgcggtctaa tatgacaata tcgtctcggg ttaaattcaa tcgttgtaaa
     7261 aagtatgcaa taaattccgt tttgttatac aacaccgcat cttcaaacac atatatagag
     7321 ctgtctccat caatatattc gttataagcg atggaaccat cttcattata gaattgtcgc
     7381 atatataatt tcgctttatt atcagctggt gcataatact cagaaaatat acgcgtataa
     7441 ctataaaaat ctttacgtac taacatacta ttaattacaa attctgcacg atccacaaca
     7501 tctttttgtt cattttgtag ataacatgtt acaaatgatg atttcccatt aaaatataga
     7561 cggactatct taccatttct ttctctaaaa ctaatttcat gaccaagctc acgttcaatg
     7621 tcatctaacg tgtacgttgt tggtgctata gaaatatcac taaaatactg atacaaccaa
     7681 ataacttctt gatctttaaa cccaatgttt tgcgttaatg tctgtatgtt ctctgactgt
     7741 ataaaatcta aaaacacaaa tttagtgtct tgatttgtac gtctcaataa tttagcacgg
     7801 taagcttgtg catattcaac accgctactc gcccagccta taccaaagtt tatattatat
     7861 attgtcatgc gctacccctt ttcatttatg gaaaatgtat aactggcata ccctctttat
     7921 caaatgtaat catgctttga caaatatttt tcaccattct ttttttgata tttcgtgtca
     7981 taacttcaaa tgaatctaat gcaactctat ggtattcaaa aatagcgtta cgttgcccat
     8041 tttgtcgttg attaacgctt gcttttaatt gttgtaaata gtcgacttgt tctaaccaac
     8101 atgaatcaat tgctttcaaa aagacttttt gaacgaaaat attataataa tatgcacttt
     8161 gcatgttttt acgattcaaa gctaattgct tttcaaattg ctctaataaa aatgtcacta
     8221 ctgcttgctt atctttaaaa ttaacacaag ccacatcttt attaaattgg aaacttaaat
     8281 tttgataaat atactcgaca acacgcgatt ttgttagcac cttttcctca tttacaaaca
     8341 tttcaaatac atctttagct aacgctttaa aatctcgatt ctcagcatca tctatttcta
     8401 aaactcgatt gcgttcctcg tatacaagat ctcgctgtat actaatgctt ttttcaaatt
     8461 cattagccat ttcacgagct ttaacccctt gttcttccga gatacgctgc gctttaacta
     8521 caatttgctt aactttgcga ttaaacaaat tactttgcga taatcgttgt gcatctaatg
     8581 aatataattg attattttcc gctaaattac tatcgctcca tcgcttaact aaataatcat
     8641 ctagtgaaat atatatacaa gatgatcccg gatccccttg tctaccagaa cgaccacgta
     8701 attgcctgtc tacacggcta ttttccatat gttcatgaat aataacagct aatccaccta
     8761 atgcttcgac accttcacca agtttaatat ctgtgcctcg acctgccata ctagtcgcaa
     8821 cagtcatgga accaatttgc cctgcttcag ctatcatctg cgcttctttt gcaacatttt
     8881 gcgcaatgag taaattatta ggaatatcca tttggaataa tactttcgaa aagtattcag
     8941 ccgcttcagc agttctcgtt atgagtaaaa ccggtcgccc cgtttcatga agttcaacta
     9001 tatcatgaat catcgcgatg tttttctcat caactgaacg aaacacttta tctggttcat
     9061 cgatacgttg aatcgcttta tcagttggtg cttgtacgac tatttttgaa tacaaatcaa
     9121 agaactctga ttcgcctaat tttcctgtag ctgtcatacc tgaaaatgat tcaaaaagtt
     9181 taaataaatt ctggaaggta attgttgcca taacactttt atctgttgaa acctccatac
     9241 cttctttcgc ttcaatagct tggtgaagtc cagcttgcaa cttagttccc ggtaacatac
     9301 gacctgtaat acggtcaatt aaaacaatat caccattata tacaaagtaa tcgacattag
     9361 attcaaacaa atattgtgcg cgcagtgcta aattaatatt acgcactagg accatcgctt
     9421 gttcgctata taaatcttca acattaaagt atgattgtgc cgcttcaata ccttgattta
     9481 acagccatat ttcttttttg gtcttcttca ttttaaaatg cacgtcttca atcaatgtat
     9541 ctacaaactc tttcacaata tgaaatagat ttgattgtaa tcttggtgca cccgaaataa
     9601 ctaatggtgt ttgagcagca tctaaaatga ttgaatccac ttcatcaata ataccgtaat
     9661 ttaattgtgg taaaaatttc ccttccgcac tatcagccaa attatcaatt aaataatcaa
     9721 aaccgagacg tccattagtt gtatatataa tatcatgttc atatatatta cgtttttccc
     9781 ctttttgata ctcataatcc acaatatcaa caaaacctaa tgaagcagtt aaacctaacc
     9841 attcatataa tggttgcatc tcttcaaaat ctcgcttcgc taaataatca ttcgttgtaa
     9901 ttaaatatgt tccttttccc gaaagagcat ttaaatataa aggcatcgtt gccgttaatg
     9961 ttttaccttc gcctgtttgc atctccgcaa tgttaccttc atgcaataca atcgctccga
    10021 ttaactgaac ttctttagga tacataccta atactctcca gctcgcttca cgtgccactg
    10081 cataagcttc aggtaacaat gtatctagtg tatcaactcc tgatgctaaa cgttctttaa
    10141 attctattgt cttttgtttt aacgcatcat cagaatatga tttaacttca tcgctccatg
    10201 tattgatgcg tttcactatt tttctaatcg actttagtct taattcgttt atcgtaacat
    10261 ctagtttatg tttcatttac ttccccacca ttcagtttcg atacatctaa gtaatctaaa
    10321 aatcgtactg gattcattaa acgtgacata taatttagat ggttgtcttg ctcttcttta
    10381 aaataaaact cgacatttgt atcttttagt tcattatttc ctgggacatg ttctgtaagc
    10441 cattctttta aatcatcatc ttcatggctt gtacgatata ctttgcaatc taaatgctga
    10501 gcgacataag ttgcaaaaac atttgacttt gacccataac taatcaaatt aatagccgtt
    10561 agggtatctt gactttgcaa atcatccttt agttgcttaa tattttcctt gatatcgtcg
    10621 tccatccaac gttcaacgag ccaaacatga ccaaacagtt tcaaaaaatc attcgaaata
    10681 gttggatagg tgtcagatgg ttctgcaata atgacattga tcctatcatt tccatattgg
    10741 ttatcgtcta tcttcgtcac ccgcatgctt ttatactcta aatcatcttg atgcgtcatc
    10801 tctgtaattg ttaaacatct aaatattaga ctcgtcgatg ctgcattcat catttttatt
    10861 ttataagcat aggcttcatc aggatattga atcgtaatac tatttgactt tacaatctca
    10921 gtacttagtt ttgtgccatt tttattataa aaaatgatga taaaatacac tgaaccagca
    10981 ggcgttgcat caaaatcaaa atgcaattta taatgctgtc ctctacgcaa aattggtaaa
    11041 cttggcgcac ttttatattt tgaaaattgc tttaacatca accactcatg aatcggtaat
    11101 ccagagggca tcaaaggatt tataaaagtc acttcaccat ttgaaaatga tactttagag
    11161 ccatacataa atgtagtttg tgaaatataa ttccaagtaa ctttaaatgt tttgtttttc
    11221 agcatgttga actctcccaa acttgtcttc caaaataatg ttgtaaaaat taacaaacca
    11281 acttgcaatg gtaggtgaat catcattatg tcgcccagga atactgcgat tcatcactct
    11341 tgcttggtgt gctgtcaata caggtaatag ctcttgaaat gcatgtggat cataatcatc
    11401 atgttgcata tatgctatgg caaaaacagt ttgtgacaat gatttctttt gaaatgtttg
    11461 ccaaaatttt tgatttaatg cctttatcga cgcttgagat gtatcacctt cattagacac
    11521 caggacgtct aatgctgtac cgaactcttc tggtctaagt aatcgcatat gttcagcaat
    11581 cgttccaata ttaacaagtg gtttaccaac aataattgcc tgaggattta actgagcacc
    11641 ataatacaag gcaccaaatg aacccataga taaaccagat aatattaatt catgtgattt
    11701 aaaattcagc ttttctaatg tctcgtcaat aacattgata ataccttgtt catattcaga
    11761 tgaaccgata taaaagctac caccttcaac acgaggatcg ccgataagta aaaacggtgc
    11821 attcatacgt ttcatcatat aatatccttc gaaaccttcc gctgttcgat aaccactaaa
    11881 atatacgttt agtggcggtt tcatatcacc agggtggaaa taataaataa attcctgtcg
    11941 ttgactatct acgaaacgac taccaccaag taaaaattga cccatgtcta atctagacca
    12001 tcgtttgtgt ataggtccta aatgtaccgt cccgttccca cgcgccttaa cagttacact
    12061 tatataagca tcaaatggtt tcgcaggtat ctctaaagga ctgtctaaca tatcatcagt
    12121 taatatgatt tgttcaatta atgcaccatc agcgccagtc tgaatcaatc taaatgtata
    12181 ttgcaactcg accgcaccat caatatcaaa ttctggccat atttgaatga ctttatcttt
    12241 atcgtaaacg agattatttt gccaagatgc gataggttta aattcttttc caaattctcc
    12301 actcaacgtg agctctgaat taccttggta tacgacatct actttaaaat tcggatgcac
    12361 aagtgctaac ttaggggaaa ctttatctcc atactgtcct gagaagctaa ctgcctctaa
    12421 tttattatta cgttcttcaa tattccggta atgtaatggt tgaacaacgt atttttggac
    12481 attttcgtct tgttcatatt caactgacca aaatgattca tcaacatacg tattgtatgg
    12541 ttcgcttatc atttgtaata aattcgttaa tgtctccgag tatggtgctt gaatatagat
    12601 aaaatcaaag cgcccttctg cttcaacaat cgcttcaata gcctctacat aaccactatc
    12661 aaattcaaac aatccaatat cgaagtaatc ccaactcaca ccttttttgt gttgaaaaat
    12721 aggttctaaa tcgtctcctc caatttgcaa aactctaaat ttacgtggca tcattttcac
    12781 cttctattaa ctcatcgagc tgattaataa tattcttaga agcatatgca tctattaatt
    12841 ttaaagaata ggcgtacgca taattccaat ttttcaaata aaataaataa taatttaacg
    12901 catcatctaa ttcatcaact gtatttataa tacggccatt gtcataatca gagacgtaat
    12961 ctgtttgttg accattaatt tgtggaatcc cagcgctaat tgcactaatt tgtaaataca
    13021 aatcaggttc ttttgacata tctatcacaa gtcgcaacgt ccgcaatgct tctacaacat
    13081 catgttcagc atgtatcgtc ttaacagcaa tgatgtcatc ttgatcttca ggtgtcatta
    13141 atgctgaaac attaacatcc gcattctgtt tagcttggta ttcctcattt accgacgtaa
    13201 tacattcacg aagccacatc ggtatgtcat tttgatggcg cgataataaa attaaacggt
    13261 aataatcttc ctgtgcgata taatccacaa gtcgttgcat catttgttgc aaatcagcgt
    13321 cactcatacc atctatccat acacctataa atgtttccat caattgacta cttatattag
    13381 gtgattgtct cgtttcaaat ggtgtgattc gaatcattgt attctcccgc tgatacttct
    13441 cttgatgact aattaaatca cgttctagtt gcacactatc aacaattaaa tgggcattct
    13501 ttacgataga ttgatattcc tcatctgaca cagtttcatt tctattttta aaaaatgaat
    13561 aacttaacaa tttcgctgga atatgattgg ctatttgtcg attgtgccta gcatctgaag
    13621 ccacaatcac atgatcatct tcatgtattt gttgtgcaat cattgcttga aatttttctt
    13681 caattagttg agccatattg ttatattctg tttgttgata gtgatgttga tatctttttg
    13741 atacagtgac tctgccattt ttcaaatctt catgaagtac acaatctcca ttaatcgtta
    13801 aatattgttg gtaagaagcc tctccctgat catcaaaata acgtatcgct gataaataac
    13861 ctctgtcatc aaaaatataa cgccgttgta actgatctct ttcaaattct tcaaaccaaa
    13921 ttgaataccc ttcttgacta aaataaatat ttgtataggt ctgttcactc gtcacacatt
    13981 ttaataaata cggcgtgtac acaaactcaa catcatccgg ccattttaag tgatgataat
    14041 taatcgcttg tggcgcatgg tgactgaatc cttgaatttc atcaaacaca gacgaatact
    14101 ttgtttcata taagtcatat cgatgtaaaa atgttcttaa atttggtgca tgattgagaa
    14161 caatcagttg ataatctaag tcattttcaa ggtgcattcc cattaaacta atcatatcgt
    14221 caaattccgt cttattttgt agttgataat acggcacagt cgtgtcttgc caccatcgtt
    14281 ggtcatcgta ccaagctgga ataaagtatt tcataattac ctccttacca atactggttt
    14341 aaaaatggct tatatttatc aaaatataaa tatgtacgaa ttgtttctgc aatattaata
    14401 ctgatgtaaa ctaatacaat cagttgtact gagaaataaa tttcagtaga taaatgcggt
    14461 acaaacaatg tgaaataaag cggtatacca ataatgactg taactaatgc cgatccaaac
    14521 caacatacgc gtcttgcttg ataatttaaa taacgttctg tatccttacc aggtttaact
    14581 cctgaaaaat aattgccact ctttaagaaa tctttggatt tttgtttagt attgattaaa
    14641 aatctcgata aaaaataacc caataacatt tgaatcacta aatatactga aatacctact
    14701 ggactatcaa atgtcagcat tggcatgtca tctgatatgc ttttattaaa catagataaa
    14761 ataaaatgaa tgccactttt caagaaaaca aatgctgaaa tactcatcat taaagtaata
    14821 ctgcctgcag ggttaacttt ccaagataaa taagatttca tatttgttgc ggaaacgttc
    14881 attaaatcga tatatggtat tctcacttct actaattcaa tgaataataa gataaacaat
    14941 gtgattatca caaggatgat taacaacgca atcacaatat gacttgcatc tatatattcc
    15001 attttttgat gcatcattga tttaataatg ctaaccatta caatcggcat tggtcctgcg
    15061 atgccgtaac gactattttt gtcagctaac caaactaata acatcgttcc agtaaccaaa
    15121 atcaatattg ttaagtaaat attgtcttga tgaacacgtt ctttcgaaac atattcatga
    15181 atcacaaaat aactttgaat cacacttaaa attaatgtta agatacgctc tttataatgc
    15241 ttctcaagac ttgtttgttt catgtattta tccatattcc gataagaaat cagcatcaag
    15301 ataatcattg atgttaacca cggaactaat cctaaagtga aaatgtttaa agtattaacg
    15361 tctccaccca tattagaaat agctatttta aaaaatgact catgttttac ttgcatatcg
    15421 ttataggaaa cgatggaaa
//
LOCUS       DAHHSC010000046        15160 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.046, whole genome shotgun sequence.
ACCESSION   DAHHSC010000046 DAHHSC010000000
VERSION     DAHHSC010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 15160)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15160)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15160
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.046"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            231..434
                     /locus_tag="NBI57_001089"
     CDS             231..434
                     /locus_tag="NBI57_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1822049.1"
                     /translation="MQLSHSVKVAISIYLALIFITFTSYLVIILYTSMTGHDVSHFVL
                     DSQHSHHGSLTQKHLSLPEISFK"
     gene            617..1552
                     /locus_tag="NBI57_001090"
     CDS             617..1552
                     /locus_tag="NBI57_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HCD1822050.1"
                     /translation="MRYLANNHSALRLKGIILAIVGACLWGLGGTVSDFLFKYKNINV
                     DWYVTARLVVSGVFLLIMYKMMQPKRSIFSVFQDRRMLGKLLIFSILGMLVVQYAYMA
                     SINTGNAAIATLLQYIAPVYIIIWFVIRGVAKLTLFDVLAIIMTLLGTFLLLTNGSFS
                     NLVVNPASLFWGILAGVALAFYTIYPSDLLNRFGSILIVGWAMLISGVAMNLRHPIWH
                     IDITKWDISIILFLIFGIIGGTALAFYFFIDSLQYISAKETTLFGTVEPVVAVIASSL
                     WLHVAFKPFQIVGIILIMILILLLSLKRQPEALDE"
     gene            complement(1633..2601)
                     /locus_tag="NBI57_001091"
     CDS             complement(1633..2601)
                     /locus_tag="NBI57_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCD1822051.1"
                     /translation="MRKKWSTLAFGFLVAAYAHIRIKEKRSVKSYMLEQGIRLSRAKR
                     RFMYKEEAMKALEKMAPQTAGEYEGTNYQFKMPVTVDKHYGSTVYNVNDKQDKHQRVV
                     LYAHGGAWFQDPLKIHFEFIDELAETLNAKVIMPVYPKIPHQDYQATYVLFEKLYHDL
                     LNQVADSKQIVVMGDSAGGQIALSFAQLLKEKHIVQPGHIVLISPVLDATMQHPEIPD
                     YLKKDPMVGVDGSVFLAEQWAGDTPLDNYKVSPINGDLDGLGRITLTVGTKEVLYPDA
                     LNLSQLLSAKGIEHDFIPGYYQFHIYPVFPIPERRRFLYQVKNIIN"
     gene            complement(2722..3078)
                     /locus_tag="NBI57_001092"
     CDS             complement(2722..3078)
                     /locus_tag="NBI57_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000240663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HCD1822052.1"
                     /translation="MVKQLNSVEAFREFIHQYPLAVVHVMRDQCSVCHAVLPQIEDLM
                     QSYPNVPLAVINQSQVEAIAGELNIFTVPVDLIFMNGKEMHRQGRFIDMQRFEHHLKQ
                     MNDSVNNDVDEHEYRK"
     gene            complement(3120..3530)
                     /locus_tag="NBI57_001093"
     CDS             complement(3120..3530)
                     /locus_tag="NBI57_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HCD1822053.1"
                     /translation="MTTEGLLVAEKEIEVNGYDIDAMGVVSNIVYIRWFEDLRTAFIN
                     QHMNYSTMINQGISPILMKTEAEYKVPVTIHDKPVGRIYLVKASKMKWVFQFEIVSAH
                     CVHCIGTQTGGFYRLSDKKITSVPQVFQDILATK"
     gene            complement(3910..5976)
                     /locus_tag="NBI57_001094"
     CDS             complement(3910..5976)
                     /locus_tag="NBI57_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIABC"
                     /protein_id="HCD1822054.1"
                     /translation="MFKKLFGQLQRIGKALMLPVAILPAAGILLAFGNAMHNEQLVEI
                     APWLKNDIIVMISSVMEAAGQVVFDNLPLLFAVGTALGLAGGDGVAALAALVGYLIMN
                     ATMGKVLHITIDDIFSYAKGAKELSQAAKEPAHALVLGIPTLQTGVFGGIIMGALAAW
                     CYNKFYNITLPPFLGFFAGKRFVPIVTSVVAIATGVLLSFAWPPIQDGLNSLSNFLLN
                     KNLTLTTFIFGIIERSLIPFGLHHIFYSPFWFEFGSYTNHAGELVRGDQRIWMAQLKD
                     GVPFTAGAFTTGKYPFMMFGLPAAAFAIYKNARPERKKVVGGLMLSAGLTAFLTGITE
                     PLEFSFLFVAPVLYGIHVLLAGTSFLVMHLLGVKIGMTFSGGFIDYILYGLLNWDRSH
                     ALLVIPVGIVYAIVYYFLFDFAIRKFKLKTPGREDEETEIRNSSVAKLPFDVLDAMGG
                     KENIKHLDACITRLRVEVVDKSKVDVAGIKALGASGVLEVGNNMQAIFGPKSDQIKHD
                     MAKIMSGEITKPSETTVTEEMSDEPVHVEALGTTDIYAPGVGQIIPLSEVPDQVFAGK
                     MMGDGIGFIPEKGEIVAPFDGTVKTIFPTKHAIGLESESGVEVLIHIGIDTVKLNGEG
                     FESLINVDEKVTQGQPLMKVNLAYLKAHAPSIVTPMIITNLENKELVIEDVQDADPGK
                     LIMTVK"
     gene            complement(6277..8016)
                     /locus_tag="NBI57_001095"
     CDS             complement(6277..8016)
                     /locus_tag="NBI57_001095"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HCD1822055.1"
                     /translation="MAKIKANEALVKALQAWDIDHLYGIPGDSIDAVVDSLRTVRDQF
                     KFYHVRHEEVASLAAAGYTKLTGKIGVALSIGGPGLIHLLNGMYDAKMDNVPQLILSG
                     QTNSTALGTKAFQETNLQKLCEDVAVYNHQIEKGDNVFEIVNEAIRTAYEQKGVAVVI
                     CPNDLLTEKIKDTTNKPVDTSRPTVVSPKYKDIKKAVKLINKSKKPVMLIGVGAKHAK
                     DELREFIEMAKIPVIHSLPAKTILPDDHPYSIGNLGKIGTKTSYQTMQEADLLIMVGT
                     NYPYVDYLPKKNIKAIQIDTNPKNIGHRFNINVGIVGDSKIALHQLTENIKHVAERPF
                     LNKTLERKAVWDKWMEQDKNNNSKPLRPERLMASINKFIKDDAVISADVGTATVWSTR
                     YLNLGVNNKFIISSWLGTMGCGLPGAMASKIAYPNRQAIAIAGDGAFQMVMQDFATAV
                     QYDLPLTVFVLNNKQLAFIKYEQQAAGELEYAVDFSDMDHAKFAEAAGGKGYTIKSAS
                     EVDAIVEEALAQDVPTIVDVYVDPNAAPLPGKIVNEEALGYGKWAFRSITEDKYLDLD
                     QIPPISVAAKRFL"
     gene            complement(8067..8756)
                     /locus_tag="NBI57_001096"
     CDS             complement(8067..8756)
                     /locus_tag="NBI57_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HCD1822056.1"
                     /translation="MNDYVHALLMILLTVVLYYFAKRLQQKYPNPFLNPALIASLGII
                     FVLLIFGISYNGYMKGGSWINHILNATVVCLAYPLYKNREKIKDNVSIIFASVLTGVM
                     LNFMLVFLTLKAFGYSKDVIVTLLPRSITAAVGIEVSHELGGTDTMTVLFIITTGLIG
                     SILGSMLLRFGRFESSIAKGLTYGNASHAFGTAKALEMDIESGAFSSIGMILTAVISS
                     VLIPVLILLFY"
     gene            complement(8749..9144)
                     /gene="cidA"
                     /locus_tag="NBI57_001097"
     CDS             complement(8749..9144)
                     /gene="cidA"
                     /locus_tag="NBI57_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin-like murein hydrolase modulator CidA"
                     /protein_id="HCD1822057.1"
                     /translation="MHKVQLIIKLLLQLGIIIVITYIGTEIQKIFHLPLAGSIVGLFL
                     FYLLLQFKIVPLTWVEDGANFLLKTMVFFFIPSVVGIMDVASEITLNYILFFAVIIIG
                     TCIVALSSGYIAEKMSVKHKHRKGVDAYE"
     gene            complement(9357..10235)
                     /locus_tag="NBI57_001098"
     CDS             complement(9357..10235)
                     /locus_tag="NBI57_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000351767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCD1822058.1"
                     /translation="MDIKHMKYFIEVVKQGGMTNASKSLYIAQPTISKAIKDIENEMG
                     TPLFDRSKRHLILTDAGQIFYEKSKEIVALYDYLPSEMERLNGLETGHINMGMSAVMN
                     MKILINILGAFHQQYPNVTYNLIENGGKTIEQQIINDEVDIGVTTLPVDHHIFDYTTL
                     DKEDLRLIVSREHRLAKYETVKLEDLAGEDFILFNKDFYLNDKIIENAKNVGFVPNTV
                     AQISQWHVIEDLVTNELGISILPTSISEQLNGDVKLLRIEDAHVHWELGVVWKKDKQL
                     SHATTKWIEFLKDRLG"
     gene            10402..10620
                     /locus_tag="NBI57_001099"
     CDS             10402..10620
                     /locus_tag="NBI57_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sterile alpha motif-like domain-containing
                     protein"
                     /protein_id="HCD1822059.1"
                     /translation="MSFYEFMQSFLGDDTPLGELVDWINQDSNFPREVKSHSEILSYF
                     RTNPCPESISVPVIKRALSVFNQFTNVQ"
     gene            11010..11441
                     /locus_tag="NBI57_001100"
     CDS             11010..11441
                     /locus_tag="NBI57_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HCD1822060.1"
                     /translation="MEYKKILIRLLIAFAVLFSADFTYQSVEQTHQSHAAVNYYSKNQ
                     CTWWAFKRRAQVGKPVSNRWGNAKNWYYNARKSKYATGRTPRKFAVMQSTAGYYGHVA
                     VVEQVYKNGSIKVSEYNFYRPLKYNTRVLSKKAARNFNYIY"
     gene            complement(11580..12857)
                     /locus_tag="NBI57_001101"
     CDS             complement(11580..12857)
                     /locus_tag="NBI57_001101"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HCD1822061.1"
                     /translation="MQSLDKNFRHLSRQQKLQQLVDKQWLSEDQFDILLNHPLIDEEV
                     ANSLIENVIAQGALPVGLLPNIIVDDKAYVVPMMVEEPSVVAAASYGAKLVNQTGGFK
                     TVSSERIMIGQIVFDGVDDTEKLSADIKALEKQIHKIADEAYPSIKARGGGYQRIAID
                     TFPEQQLLSLKVFVDTKDAMGANMLNTILEAITAFLKNESPQSDILMSILSNHATASV
                     VKVQGEIDVKDLARGERTGEEVAKRMERASVLAQVDIHRAATHNKGVMNGIHAVVLAT
                     GNDTRGAEASAHAYASRDGQYRGIATWRYDQKRQRLIGTIEVPMTLAIVGGGTKVLPI
                     AKASLELLNVDSAQELGHVVAAVGLAQNFAACRALVSEGIQQGHMSLQYKSLAIVVGA
                     KGDEIAQVAEALKQEPRANTQVAERILQEIRQQ"
     gene            13112..14278
                     /locus_tag="NBI57_001102"
     CDS             13112..14278
                     /locus_tag="NBI57_001102"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HCD1822062.1"
                     /translation="MTIGIDKINFYVPKYYVDMAKLAEARQVDPNKFLIGIGQTEMAV
                     SPVNQDIVSMGANAAKDIITDEDKKKIGMVIVATESAVDAAKAAAVQIHNLLGIQPFA
                     RCFEMKEACYAATPAIQLAKDYLATRPNEKVLVIATDTARYGLNSGGEPTQGAGAVAM
                     VIAHNPSILALNEDAVAYTEDVYDFWRPTGHKYPLVDGALSKDAYIRSFQQSWNEYAK
                     RQGKSLADFASLCFHVPFTKMGKKALESIIDNADETTQERLRSGYEDAVDYNRYVGNI
                     YTGSLYLSLISLLENRDLQAGETIGLFSYGSGSVGEFYSATLVEGYKDHLDQAAHKAL
                     LNNRTEVSVDAYETFFKRFDDVEFDEEQDAVHEDRHIFYLSNIENNVREYHRPE"
     gene            complement(14443..14964)
                     /locus_tag="NBI57_001103"
     CDS             complement(14443..14964)
                     /locus_tag="NBI57_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HCD1822063.1"
                     /translation="MEYKSYYDSPVGRLELLSDGVSLTAVLFENQQGDGTREENTSLA
                     IFREATQWLDAYFKGDNPEITIPLKPTGSHFQQCVWNELRQVPYGTLTTYGAIAKKVG
                     KVLDKPKMSAQAVGGAVGSNPLSIIVPCHRVVGKTGSLTGFGGTINNKIKLLELENID
                     MSKLYIPKHSTKP"
BASE COUNT         4728 a         2901 c         2324 g         5207 t
ORIGIN      
        1 ttttgtgctg tgtttataaa accgcacact atattcaaaa ataacttatt acacatatat
       61 ttgcaataag ataattaaag taggatatta tttttagttt tctgatagga atgatgatag
      121 tctataggtt gaatctttac tttttttaaa gctaaattta catcaactta acaatggctg
      181 gttatactga agatgaaaat atttagtata acttagtgga ggcgataaag gtgcaattga
      241 gtcattccgt taaagttgca atttctatct atttagcact tatctttata acgttcactt
      301 cttatttagt cattatttta tatacgagta tgactggaca tgatgtatca catttcgtgt
      361 tagatagtca gcattctcat catggatctc ttacgcaaaa acatttgagt cttcctgaaa
      421 tctcatttaa atagtttatc ctctgtgttt caacattcat ttcccatatc gattcattta
      481 tctatcatct agaccactac atcttagatg atttttttat tttctcattt cactctttct
      541 taaaagtcga tataatgaat taaatcatta tcatacaccg acatatttta tgttgttggt
      601 gttaagtttt aaaggggtga gatacttggc gaataatcat tcagctttgc gtttaaaagg
      661 aattatactt gccattgtcg gtgcttgttt atggggatta ggtggtactg tttctgattt
      721 cttgttcaaa tataagaata ttaatgtcga ttggtacgtc actgctcgac ttgtagtcag
      781 tggtgttttc ttacttatta tgtacaaaat gatgcaaccc aaacgttcaa tatttagcgt
      841 attccaagat cgacgtatgt taggcaaatt acttatcttc agtatactgg gcatgttagt
      901 tgtacaatat gcttatatgg catctattaa tacaggtaat gctgcgattg caacattact
      961 acaatacatt gcgccagttt atattattat ttggtttgtc ataagaggcg ttgcaaaact
     1021 aacattattt gatgtgcttg ctattatcat gacactatta ggaacatttt tattattaac
     1081 gaatggttca ttttctaatt tagtcgtcaa tcctgcaagt ttattctggg gtattttagc
     1141 tggtgtagca ctcgcttttt acacaattta tccttcagac ctacttaacc gcttcggttc
     1201 gattctaatt gtcgggtggg caatgcttat ttctggtgtt gcgatgaatt tacgccatcc
     1261 aatttggcac attgatatca ctaaatggga catatcaatt atattatttt taatctttgg
     1321 tattatcggt ggtaccgcac tcgcatttta tttctttatc gacagtttac aatacatatc
     1381 agcgaaagaa acaacattat tcggaactgt tgaacctgtc gtagccgtta tcgcaagcag
     1441 tctatggtta catgtggcat tcaaaccatt tcaaatcgta ggcatcattc ttattatgat
     1501 tttaatttta ttactatcac ttaaaagaca acctgaagca ttagatgaat aagaaaactc
     1561 tgataatcac tttagcaagt aactattttt aacaacgtag ctaccttgta tgtgatatca
     1621 gagtttttta ttttaattaa taatattttt cacttggtat aaaaagcgtc ggcgctctgg
     1681 tatcggaaat actggataaa tatggaattg gtaatatcca ggtataaagt catgttcgat
     1741 accttttgca ctcaacaatt gcgataagtt caaagcatct gggtacagta cttcttttgt
     1801 gccaacagtt aatgtaatac gacctaaacc atctaagtca ccattaattg gtgatacttt
     1861 gtagttatct aaaggtgtgt cccctgccca ttgttcagct aagaacacac tgccatccac
     1921 acctaccatt gggtctttct ttaagtagtc aggaatttca ggatgctgca tcgttgcatc
     1981 taaaactggt gaaattaata caatatgtcc tggttgcaca atatgttttt cttttaacaa
     2041 ttgagcaaat gataaagcaa tttgaccgcc cgcagagtca cccattacaa cgatttgttt
     2101 agaatctgct acttgattca ataaatcatg gtataacttt tcaaaaagca catacgtcgc
     2161 ttgataatct tgatgcggaa tcttcggata tactggcatg atgactttag cattgagtgt
     2221 ttctgcaagt tcatcaataa attcgaaatg aattttgagt gggtcttgga accatgcgcc
     2281 tccatgtgcg tataatacaa cgcgttgatg cttatcttgt ttatcattaa cgttataaac
     2341 ggttgaaccg tagtgcttat ccactgttac tggcatctta aactgataat tggtgccctc
     2401 atattcacct gctgtctgtg gcgccatttt ttctaatgct ttcatcgctt cttctttata
     2461 cataaaacga cgcttagctc tagataatcg tataccttgt tctaacatat aacttttcac
     2521 actgcgtttt tctttaattc taatatgtgc gtatgctgct actaaaaatc caaacgcaag
     2581 tgtagaccat tttttacgca tttgtcgtca ctcctctttg atgatttcta ctatagttct
     2641 actgtaccat gctgattctt attactacca atatttactg ggaaataagt tatgatgtct
     2701 acataatctt agcaatgcta atcatttgcg atattcatgc tcatcgacat cgttattcac
     2761 actatcattc atttgcttaa gatgatgttc aaaacgttgc atatcgataa aacgcccttg
     2821 acgatgcatt tcttttccat tcataaaaat taaatccaca ggtacagtga aaatatttaa
     2881 ttctccagca atagcttcaa cttgactttg attaatcaca gctaatggca cattgggata
     2941 tgattgcatc aagtcttcaa tttgtggtaa aacggcatga cacacgctac actgatcgcg
     3001 catgacatgt acaactgcta acggatattg atgaataaat tcacggaatg cttcgacact
     3061 atttaattgc ttaaccattt cgaacacctt cttacttttt atattttaaa atgaagtcat
     3121 tattttgttg ctaaaatgtc ttgaaacact tgtggcacag aggttatctt cttatcactc
     3181 aatctgtaaa aaccgcctgt ctgtgtacca atacaatgca cgcaatgtgc ggacacaatt
     3241 tcaaactgaa acacccattt catcttgctt gctttaacta agtaaatacg acctactggt
     3301 ttgtcatgta ttgtgacagg tactttatac tctgcttccg ttttcataag tataggtgaa
     3361 atgccttgat tgatcattgt tgagtaattc atgtgctgat taataaacgc tgttctcaaa
     3421 tcttcgaacc atctaatata aacgatatta ctaacgacac ccatcgcatc aatgtcgtaa
     3481 ccattcactt cgatttcttt ctctgcaact aatagacctt ctgttgtcat aatgacaaat
     3541 ctccttatct tacaaataga tttaaaatca tgttaattta tttttattac tttcatgata
     3601 aaaatacaag caattgaact ttattacgga aaatcaaact tttcaatgag actattaatt
     3661 tggctagtcg cgaagatgca tttttctaaa atgtgacaaa aacataagca atgttttcta
     3721 tatctaacac taatatttaa cttattatca tcaacacacc tgctatacat cattgcgcac
     3781 taaaaaggca gtagcttcat tttaagctac tgccttttgt aacgtattct ctttcaactc
     3841 gctacttcgc atacgcacta cgaatgacta aaagttcatt catttgatat gctgtttcat
     3901 ttttaatcat tatttgactg tcataattag cttacctgga tcagcatctt gtacatcttc
     3961 aatgacaagt tctttatttt caagatttgt aataatcatt ggtgtaacga tgcttggtgc
     4021 gtgtgctttc aagtatgcta aattcacttt cattaatggt tggccttgtg ttactttttc
     4081 atcaacgtta atcagacttt cgaatccttc accattcagt ttcactgtat cgataccaat
     4141 atgaataagt acttcgacgc cactttcaga ttctaatcct atcgcatgtt tcgtagggaa
     4201 gattgttttc actgtaccat caaacggtgc tacaatttca cctttttcag ggataaagcc
     4261 aataccatca cccatcattt taccagcgaa tacttgatca ggtacttctg ataatggaat
     4321 gatttgaccg acacctggtg catagatgtc tgttgttcca agtgcttcta cgtgaactgg
     4381 ttcatctgac atttcttcag tcactgtcgt ttcacttggt ttcgtaattt caccactcat
     4441 aatcttggcc atatcatgtt taatttgatc tgattttgga ccaaagatag cttgcatatt
     4501 gtttccaact tctaatacac ctgatgcgcc taaagcttta atacctgcta catctacttt
     4561 tgatttatca accacttcta cgcgcagacg tgtaatacat gcatctaaat gtttaatgtt
     4621 ttcttttcca cccattgcat ctaagacatc aaatggtaat tttgcgacac tagagttacg
     4681 aatttcagtt tcttcatctt cacgacctgg tgttttcaat ttaaacttac gaattgcaaa
     4741 gtcgaataag aagtaataca cgatagcata tacaataccg actggaataa ctaataatgc
     4801 gtgtgaacga tcccagttta ataaaccata taaaatataa tctatgaaac cacctgagaa
     4861 tgtcatacca attttaacgc ctaataaatg cattactaag aatgatgtac cagctaataa
     4921 tacgtgaatt ccataaagta ctggtgctac aaataagaat gaaaattcta atggctcagt
     4981 gataccagtt aaaaatgcag ttaatcctgc tgataacatt aaaccaccca cgactttttt
     5041 gcgttctggt cgtgcatttt tataaatagc aaatgccgcc gctggtaaac caaacatcat
     5101 aaatggatat ttaccagtag taaatgcacc agcagtaaat ggtacgccat ctttcaattg
     5161 tgccatccaa atacgttggt caccacgaac taattcacct gcgtgatttg tataacttcc
     5221 gaattcaaac cagaacggtg aatagaaaat atgatgtaaa ccaaatggaa ttaatgagcg
     5281 ttcaataata ccgaatataa acgttgttaa tgttaaattt ttatttaata agaaattcga
     5341 taaactattt aatccatctt gaattggtgg ccacgcaaag cttaaaagca cacctgttgc
     5401 gattgctacg accgatgtca caatcggtac aaatcgttta cctgcaaaga atcctaaaaa
     5461 tggtggtagt gtaatattat aaaatttgtt gtaacaccat gcggctaaag cacccatgat
     5521 aataccacca aacacacccg tttgtaacgt tggaatacct aatactaaag catgtgctgg
     5581 ttctttcgct gcttgactta attcttttgc ccctttggca tatgagaaaa tgtcatcaat
     5641 tgtaatgtgc aacactttcc ccattgttgc attcataatt aagtaaccta ctagcgctgc
     5701 taatgctgca acaccgtctc ctcctgctaa tccaagtgct gtaccaactg caaataataa
     5761 tggcaagtta tcaaatacaa cttgtcctgc tgcttccatg accgacgaaa tcattacaat
     5821 gatatcgttt tttaaccatg gtgcaatttc tactaattgt tcgttgtgca ttgcgttacc
     5881 aaacgctaat aaaataccag ctgctggtaa aatcgcaaca ggtaacatta atgctttacc
     5941 gatacgttgc aattgtccaa acaatttctt aaacatataa acatccccta tctcaatttc
     6001 cgcttccatg tacaaaaaaa gcaggcatga acaaacaaga ctattctcat agtacggttg
     6061 tttgctcatg cctgcattac cagtaacacg caagtgatat gtaattcata gctattatac
     6121 atatttgtaa aagcgttttc aacaactttt tcacaatttt atcatcaatt cagtacgatc
     6181 atttgtgata aataaacaaa acggcaattc ccattttgtt gaaatagaaa ttaccgtttt
     6241 tatagttcaa ttcaattgtg ataacctttc aatcagttat aagaaacgtt ttgctgcaac
     6301 tgaaataggt ggaatttgat ctaaatctaa atatttatct tcagtaatag atctaaatgc
     6361 ccatttacca taaccaagcg cttcttcatt tacaatttta cctggtaatg gcgcagcatt
     6421 aggatcaaca tatacatcta caatcgttgg tacatcttgt gctaatgcct cttcgactat
     6481 agcatctact tcgctagcac tcttaattgt ataaccttta ccacctgctg cctcagcaaa
     6541 ttttgcatga tccatatcag aaaaatcaac tgcatattct aattcaccag ctgcttgttg
     6601 ttcatattta ataaatgcta actgtttgtt attaagtaca aatacagtta aaggtaaatc
     6661 atattgtact gctgtagcga agtcttgcat taccatttgg aatgcaccgt caccagcaat
     6721 ggcaatcgct tgtctatttg gatatgcaat ttttgatgcc atagctcctg gaagaccgca
     6781 acccattgta cctaaccaac ttgaaatgat gaacttgttg ttcacaccaa gatttaaata
     6841 acgtgtagac caaactgttg cagtaccaac atctgctgaa attactgcat catctttaat
     6901 aaatttgtta attgatgcca ttaaacgttc tggacgtaat ggtttgctat tattattttt
     6961 atcttgttcc atccatttat cccaaaccgc tttacgttct aacgttttgt ttaaaaatgg
     7021 tctttccgca acatgtttaa tattttcagt taactgatgc aatgcaattt tactgtctcc
     7081 aacaattcct acattaatat taaaacgatg tccgatattt ttaggatttg tgtcgatttg
     7141 gattgcttta atatttttct taggtaagta atccacatat ggatagtttg taccaaccat
     7201 aattaataaa tccgcttcct gcattgtttg ataagatgtt ttggtaccaa ttttacctaa
     7261 gttaccgata ctatatggat gatcatccgg caagattgtt ttagctggta atgaatgaat
     7321 gacaggaatt ttagccattt caataaattc acgtagctca tctttcgcat gttttgcacc
     7381 tacaccaatt aacatgacag gctttttact tttattaatt agtttaaccg cttttttgat
     7441 gtctttatat tttggtgata caactgttgg tcttgatgta tctactggtt tattcgttgt
     7501 atctttaatt ttttcagtta ataagtcgtt aggacaaata acaacagcga cacctttttg
     7561 ttcatatgcc gtacgaattg cttcgttaac gatttcaaac acattgtcac ctttttcaat
     7621 ttggtgatta taaacggcta catcttcaca taatttttgt aaatttgttt cttgaaatgc
     7681 tttcgttcca agtgctgtac tattcgtttg tccagataat attaattgcg gtacattatc
     7741 cattttggca tcgtacatac catttaataa atgaattaaa ccagggccac cgatacttaa
     7801 tgccacaccg attttaccag ttaattttgt gtaaccagca gccgctaagc ttgctacttc
     7861 ttcatgacgt acatgataaa atttaaattg atctctcact gtacgtaaac tatcgacaac
     7921 tgcgtcgatt gagtctcctg gaataccata caagtgatct atatcccatg cttgtaatgc
     7981 tttaactaat gcttcatttg cttttatttt tgccattatt actaatagcc tccctttctt
     8041 agtgtctatc attttaaata tctaaattaa tagaataata aaattagaac aggtattaac
     8101 actgaactaa taactgcagt taaaatcatc ccaattgaac taaatgcacc ggattcaata
     8161 tccatttcta gtgctttagc tgtgccaaat gcatgtgacg cattcccata cgttaaacct
     8221 ttggcgatag aagattcaaa tcttccaaat cttaataaca tcgaacctaa aatactaccg
     8281 attaaacccg ttgtgataat aaaaagtacg gtcatcgtat ctgtaccacc tagttcatgt
     8341 gacacttcga tacctactgc ggctgttata gatcggggca ataacgttac aatgacgtct
     8401 ttagaatagc caaatgcttt aagtgttaag aacactaaca tgaaattcag catgacgcca
     8461 gttaatacac ttgcaaaaat gatagagaca ttgtctttaa ttttctctct atttttataa
     8521 agtgggtacg ctaaacatac gaccgttgcg tttaaaatat ggttgatcca actgccacct
     8581 ttcatatacc cgttataact aattccaaag ataagtaaga caaaaataat tcctaaagat
     8641 gcaattaatg ctggattcaa aaatgggttc ggatattttt gttgtaacct tttagcgaaa
     8701 taatataaaa cgacagtcaa caaaatcatt aataaggcgt gcacgtaatc attcataagc
     8761 gtctacacct ttacgatgtt tatgtttaac agacattttt tcagcaatat aacctgaaga
     8821 taatgcaacg atacatgttc ctataataat gactgcgaaa aagagtatat aatttagcgt
     8881 aatttcggaa gcaacatcca taatacctac aactgacggt atgaagaaaa agaccatcgt
     8941 ctttaataaa aagtttgcac cgtcttctac ccaagttagc ggtacaatct taaattgtaa
     9001 tagtaaataa aataaaaata gaccaacaat actgccggct aagggaagat gaaaaatctt
     9061 ttgaatttct gtgccaatat aagtaatcac aatgatgatt cctagttgta gtagtagttt
     9121 gattattaat tggactttgt gcatggcgcc atccctttct aaatatgtct aaaatgttac
     9181 aataactatt ataaagatgg cgtgcacaat ataaaaatac tcttttttta tagtttgtat
     9241 tactattttc tatggaaacg ctctctcaat ttcacttcat tttcacaaaa aaacactcat
     9301 catttcaatg acccacgtaa tgatgcgcct taaataatga gtgctttatt aatatgttag
     9361 cctaaacgat ctttcaaaaa ttctatccat ttcgttgtgg catgacttaa ttgtttatcc
     9421 ttcttccaaa caacacctaa ttcccaatgt acatgcgcat cttcaatgcg tagcagcttc
     9481 acatctccat ttagttgctc tgaaattgat gttggtaaaa tactaatacc taattcattc
     9541 gtaactaaat cttctataac atgccattgt gaaatttgcg ctacagtatt cggaacaaag
     9601 ccaacgtttt tagcattttc aataatttta tcattcaagt aaaagtcttt attaaataaa
     9661 atgaagtctt cacctgctaa atcttcgagt ttaacagttt catattttgc gagtcgatgc
     9721 tctctgctca cgataagtcg caaatcttcc ttatctaggg tagtataatc gaaaatatga
     9781 tgatcgactg gcaaagtggt cacgcctata tctacttcat cattgataat ttgctgctca
     9841 atcgttttgc cgccattttc tattaaatta tatgtaacat ttggatattg ctgatggaat
     9901 gcaccaagaa tattgataag aatcttcata ttcatgactg ccgacatgcc catgtttata
     9961 tgccctgttt ccagtccatt caagcgttcc atttcagatg gtaaataatc atacagtgca
    10021 acaatttctt tacttttctc ataaaaaatt tgacctgcat cggtaagtat taaatgtctt
    10081 ttacttctat caaataaggg cgtgcccatt tcattttcaa tatctttaat tgctttacta
    10141 attgtaggtt gtgcaatata taatgatttg gaggcattag tcatgcctcc ttgcttaacg
    10201 acttcaataa aatatttcat atgtttgata tccactaaca tcatcctaac tatttcaaca
    10261 cgtttatttc attatacaca acttcttaaa cattcaataa atttagattt ataaaattgc
    10321 tttataaaaa gttttatgta aaatacacat tcttttatgg tattatatta atggctcaag
    10381 tttgttattc gaggtgaggt attgagcttt tacgaattta tgcaaagttt tcttggtgat
    10441 gacacaccat taggtgaatt ggttgattgg attaatcaag atagcaattt ccctagagaa
    10501 gtgaagagcc atagtgaaat attgtcatat tttcgaacaa atccatgtcc tgaaagcatc
    10561 tcagtacctg taattaaacg ggcactatca gttttcaacc aattcacaaa tgtacaatga
    10621 tacatgaata catggagtca catcatctca tgttagcgct ctgattgcag ggcgctattt
    10681 ttaatgttat cgctacattt agtatattgt gattgctatt ttctttcagt tatagaacca
    10741 cgtacctttt cttttaaatc attttatatt ttcccactaa tatccgctgt taatcaattc
    10801 tgacatcctt gtatcactat gacaattaat tgttaaatac atgaatttct acattttatg
    10861 aaaaaatcca tttttattac aattcaacac tttatatgac aacttcatta cagttacttt
    10921 tattgttgat tgcttgcatt gttttctaaa aaaattttgt tatcataatt aacgttgaat
    10981 aaagaaaaaa attaacttgg gagataaaaa tggaatataa aaagatacta attcgtttat
    11041 taattgcttt tgcagtactt ttctcagcag atttcactta tcaatccgtc gaacaaacgc
    11101 atcaatcgca tgccgcagtt aattattata gtaaaaacca atgtacatgg tgggcattta
    11161 aacgtcgcgc acaagtcggc aaacctgttt ctaatagatg gggcaatgct aaaaattggt
    11221 attacaatgc acgtaaatca aaatatgcga ctggtcgtac accaagaaaa tttgctgtca
    11281 tgcaatcaac tgcaggatat tatggacatg tcgcagttgt tgaacaagta tataaaaacg
    11341 gtagtattaa agtttcagaa tacaactttt atcgcccatt aaaatacaat acacgtgtac
    11401 taagcaaaaa ggcagcacgt aactttaact atatttacta atcaaaaaac ttctatcacg
    11461 aacgcttcaa tttcctgtat gcgtgtgata gaagttttta ttttttgttc atattaattt
    11521 atctaagcgc tacgatggac tgacttaaac ttttctttaa aatcgctatt cgccatttac
    11581 tattgttgtc taatttcttg taaaatgcgt tcagctactt gtgtattcgc acggggttct
    11641 tgcttcaatg cttcagctac ttgcgcaatt tcatcacctt ttgcacctac aacaatagct
    11701 aaagatttat attgcaagct catatggcct tgctggatac cttcggaaac gagcgcgcga
    11761 catgctgcaa agttctgtgc taaaccaacg gcagcaacta catgacctaa ttcttgtgct
    11821 gaatctacat ttagcaattc taaagaagct ttagcaattg gtaatacttt tgtaccaccg
    11881 ccaacgattg ccaatgtcat aggcacttct attgtaccaa ttaaacgttg acgtttttga
    11941 tcgtatctcc atgttgcaat accacgatac tgtccgtcac gactcgcgta tgcatgcgca
    12001 cttgcttctg caccacgcgt atcatttcct gttgctaaaa caacggcatg tatgccattc
    12061 ataacacctt tattatgtgt tgcagcacga tgaatatcaa cttgtgccaa tacagaagca
    12121 cgttccattc gtttggcaac ctcttctcca gttctctcgc cccttgctaa atctttaacg
    12181 tcaatttcgc cttgaacttt aacaacggac gctgttgcat gattggataa aatactcatt
    12241 aaaatgtcgc tttgtggaga ttcatttttt aaaaatgcag ttatggcctc taaaatcgta
    12301 ttaagcatat tagcgcccat agcatctttc gtatcaacaa atacttttaa agatagtaac
    12361 tgttgctcag gaaatgtatc aatagctata cgttggtaac caccaccacg cgctttaata
    12421 gaaggatatg cctcatccgc aattttatga atttgctttt ctaaagcttt aatgtctgct
    12481 gataattttt cagtatcgtc aacgccatca aagacgattt gacctatcat aatacgttca
    12541 gaagataccg ttttaaatcc gccagtctga ttcactagct ttgcaccata actagctgca
    12601 gcgacaactg aaggctcttc caccatcata ggtacaacat atgccttatc gtccacaatg
    12661 atattcggta ataatccaac gggtaatgca ccttgcgcga tgacattttc aattaaacta
    12721 tttgctactt cctcatcaat taatggatga ttcaataaaa tgtcgaattg atcttctgat
    12781 aaccattgct tatctaccaa ttgttgtaac ttttgttgac gagataaatg tcggaaattc
    12841 ttatctaaac tttgcatgga catactcctt ttacttcaca taatttttta acatttcaat
    12901 cactactatt tttaccataa aataacgtta ttcgtcttaa aattcaattg aataattatt
    12961 gttttaacta taaaataaag caaggtaaat taaaacgctt acaagaaacg tcaaatcatt
    13021 tttaaattta gtgtatttct ttgtataaga taagcatatt ctgatatgat acaagtgttg
    13081 cttttataaa tttgaaagga tgtaaaacct tatgacaata ggtatcgaca aaataaactt
    13141 ttacgttcca aagtactatg tagacatggc taaattagca gaagcacgcc aagtagaccc
    13201 aaacaaattt ttaattggaa ttggtcaaac tgaaatggct gttagtcctg taaaccaaga
    13261 catcgtttca atgggcgcta acgctgctaa ggacattata acagacgaag ataaaaagaa
    13321 aattggtatg gtaattgtgg caactgaatc agcagttgat gctgctaaag cagccgctgt
    13381 tcaaattcac aacttattag gtattcaacc ttttgcacgt tgctttgaaa tgaaagaagc
    13441 ttgttatgct gcaacaccag caattcaatt agctaaagat tatttagcaa ctagaccgaa
    13501 tgaaaaagta ttagttattg ctacagatac agcacgttat ggattgaatt caggcggcga
    13561 gccaacacaa ggtgctggcg cagttgcgat ggttattgca cataatccaa gcattttggc
    13621 attaaatgaa gatgctgttg cttacactga agacgtttat gatttctggc gtccaactgg
    13681 acataaatat ccattagttg atggtgcatt atctaaagat gcttatatcc gctcattcca
    13741 acaaagctgg aatgaatacg caaaacgtca aggtaagtcg ctagctgact tcgcatctct
    13801 atgcttccat gttccattta caaaaatggg taaaaaggca ttagagtcaa tcattgataa
    13861 cgctgatgaa acaactcaag agcgtttacg ttcaggatat gaagatgctg tagattataa
    13921 ccgttatgtc ggtaatattt atactggatc attatattta agcctaatat cattacttga
    13981 aaatcgtgat ttacaagctg gtgaaacaat cggtttattc agttatggct caggttcagt
    14041 tggtgaattt tatagtgcga cattagttga aggctacaaa gatcatttag atcaagctgc
    14101 acataaagca ttattaaata accgtactga agtatctgtt gatgcatatg aaacattctt
    14161 caaacgtttt gatgacgttg aatttgacga agaacaagat gctgttcatg aagatcgtca
    14221 tattttctac ttatcaaata ttgaaaataa cgttcgcgaa tatcacagac cagagtaatc
    14281 tgtgtatttg aaagacattt aaatataacc taaaaacagc agtaagacag cttctcattg
    14341 aaatcgtctt actgctgttc tctatttata acacttcgta ttgaatgaat tcattatgcc
    14401 tatttgacac attattgaaa tttttaatat accccttata cgttacggct ttgtactgtg
    14461 tttaggtata taaagctttg acatatcgat attctccaac tctaacagct taattttatt
    14521 attaatcgtt ccaccgaatc ctgttaagct acccgtttta ccgacaacac gatgacatgg
    14581 cacgataatt gataatggat tacttccgac tgcacctcca accgcttggg ctgacatttt
    14641 tggcttatca agcaccttac ctactttttt ggcaatagca ccatacgttg ttagagtccc
    14701 ataaggaacc tgtcttaatt cattccaaac acactgttga aaatgactac ctgttggctt
    14761 taaaggtatt gtgatttcag gattgtcacc tttaaaatac gcgtctaacc actgtgtcgc
    14821 ctctctaaat atcgctaaag acgtattttc ttccctagta ccatcacctt gttgattttc
    14881 aaacaaaaca gcggtcagac ttaccccatc actcaaaagt tccaatcgtc ctacaggcga
    14941 atcatagtaa ctcttatact ccataaaaat tccccctttt tctaatcata cgccttaatt
    15001 gttaaattag aaaatattca tgttcacatt ttttaaaaat aaataagtcg tcaattacgt
    15061 atataaacac gtaataccag ctatcccttt gctgcaatat acagttacat aacttactac
    15121 acgtgctaac ctattacttt gtaaaccaaa tcttaaatta
//
LOCUS       DAHHSC010000047        15117 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.047, whole genome shotgun sequence.
ACCESSION   DAHHSC010000047 DAHHSC010000000
VERSION     DAHHSC010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 15117)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15117)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15117
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.047"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            156..848
                     /locus_tag="NBI57_001104"
     CDS             156..848
                     /locus_tag="NBI57_001104"
                     /EC_number="2.5.1.n9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heptaprenylglyceryl phosphate synthase"
                     /protein_id="HCD1822064.1"
                     /translation="MYDIKKWRHIFKLDPAKHISDDDLDAICMSQTDAIMIGGTDDVT
                     EDNVIHLMSRIRRYPLPLVLEISNIESVMPGFDFYFVPTVLNSTDVAFHNGTLLEALK
                     TYGHSIDFEEVIFEGYVVCNADSKVAKHTKANTDLTTEDLEAYAQMVNHMYRLPVMYI
                     EYSGIYGDVSKVQAVSEHLTETQLFYGGGISSEQQATEMAAIADTIIVGDIIYKDIKK
                     ALKTVKIKESSK"
     gene            845..3037
                     /gene="pcrA"
                     /locus_tag="NBI57_001105"
     CDS             845..3037
                     /gene="pcrA"
                     /locus_tag="NBI57_001105"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HCD1822065.1"
                     /translation="MNALLNHMNTEQSEAVKTTEGPLLIMAGAGSGKTRVLTHRIAYL
                     LDEKDVSPYNVLAITFTNKAAREMKERVQKLVGDQAEVIWMSTFHSMCVRILRRDADR
                     IGIERNFTIIDPTDQKSVIKDVLKNENIDSKKFEPRMFIGAISNLKNELKTPADAQKE
                     ATDYHSQMVATVYSGYQRQLSRNEALDFDDLIMTTINLFERVPEVLEYYQNKFQYIHV
                     DEYQDTNKAQYTLVKLLASKFKNLCVVGDSDQSIYGWRGADIQNILSFEKDYPEANTI
                     FLEQNYRSTKTILNAANEVIKNNSERKPKGLWTANTNGEKIHYYEAMTERDEAEFVIR
                     EIMKHQRNGKKYQDMAILYRTNAQSRVLEETFMKSNMPYTMVGGQKFYDRKEIKDLLS
                     YLRIIANSNDDISLQRIINVPKRGVGPSSVEKVQNYALQNNISMFDALGEADFIGLSK
                     KVTQECLNFYELIQSLIKEQEFLEIHEIVDEVLQKSGYREMLERENTLESRSRLENID
                     EFMSVPKDYEENTPLEEQSLINFLTDLSLVADIDEADTENGVTLMTMHSAKGLEFPIV
                     FIMGMEESLFPHIRAIKSEDDHEMQEERRICYVAITRAEEVLYITHATSRMLFGRPQS
                     NMPSRFLKEIPESLLENHSSGKRQTIQPKAKPFAKRGFSQRTTSTKKQVSSSDWNVGD
                     KVMHKAWGEGMVSNVNEKNGSIELDIIFKSQGPKRLLAQFAPIEKKED"
     gene            3041..5044
                     /gene="ligA"
                     /locus_tag="NBI57_001106"
     CDS             3041..5044
                     /gene="ligA"
                     /locus_tag="NBI57_001106"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HCD1822066.1"
                     /translation="MADLSSRVNELHDLLNQYSYEYYVEDNPSVPDSEYDKLLHELIK
                     IEEEHPEYKTVDSPTVRVGGEAQASFKKVNHDTPMLSLGNAFNEDDLRKFDQRIREQI
                     GNVEYMCELKIDGLAVSLKYVDGYFVQGLTRGDGTTGEDITENLKTIHAIPLKMKEPL
                     NVEVRGEAYMPRRSFLRLNEEKEKNDEQLFANPRNAAAGSLRQLDSKLTAKRKLSVFI
                     YSVNDFTDFNARSQSEALDELDKLGFTTNKNRARVNNIDGVLEYIEKWTSQRESLPYD
                     IDGIVIKVNDLDQQDEMGFTQKSPRWAIAYKFPAEEVVTKLLDIELSIGRTGVVTPTA
                     ILEPVKVAGTTVSRASLHNEDLIHDRDIRIGDSVVVKKAGDIIPEVVRSIPERRPEDA
                     VTYHMPTHCPSCGHELVRIEGEVALRCINPKCQAQLVEGLIHFVSRQAMNIDGLGTKI
                     IQQLYQSELIKDVADIFYLTEEDLLPLDRMGQKKVDNLLAAIQQAKDNSLENLLFGLG
                     IRHLGVKASQVLAEKYETIDRLLTVTEAELVEIHDIGDKVAQSVVTYLENEDIRALIQ
                     KLKDKHVNMIYKGIKTSDIEGHPEFSGKTIVLTGKLHQMTRNEASKWLASQGAKVTSS
                     VTKNTDVVIAGEDAGSKLTKAQSLGIEIWTEQQFVDKQNELNS"
     gene            5057..6256
                     /locus_tag="NBI57_001107"
     CDS             5057..6256
                     /locus_tag="NBI57_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CamS family sex pheromone protein"
                     /protein_id="HCD1822067.1"
                     /translation="MKRTLVLLITAIFILAACGNHKDDQAGKDNQKHNNSSNQVKEIA
                     TDKNVQGDNYRTLLPFKESQARGLLQDNMANSYNGGDFEDGLLNLSKEVFPTDKYLYQ
                     DGQFLDKKTINAYLNPKYTKREIDKMSEKDKKDKKANENLGLNPSHEGETNPEKIAEK
                     SPAYLSNILEQDFYGGGDTKGKNIKGMTIGLAMNSVYYYKKEKDGPTFSKKLDDSEVK
                     KQGKQMASEILSRLRENDDLKDIPIHFAIYKQSSEDSITPGEFITQATAEKSQTKLNE
                     WHNINEKSALLPSSTAADYDENLNNNFKQFNDNLQSYFSNFTQAVGKVKFVDKKPQRL
                     VVDLPIDYYGQAETIGITQYVTEQANKYFDKIDNYEIRIKDGNQPRALISKTKDDKEP
                     QVHIYSN"
     gene            complement(6345..7883)
                     /gene="putP"
                     /locus_tag="NBI57_001108"
     CDS             complement(6345..7883)
                     /gene="putP"
                     /locus_tag="NBI57_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proline symporter PutP"
                     /protein_id="HCD1822068.1"
                     /translation="MLTMGTALSQQVDANWQTYIMIAVYFLILIVIGFYGYKQATGNL
                     SEYMLGGRSIGPYITALSAGASDMSGWMIMGLPGSVYSTGLSAMWITIGLTLGAYINY
                     FVVAPRLRVYTELAGDAITLPDFFKNRLNDKNNVLKIISGLIIVVFFTLYTHSGFVSG
                     GKLFESAFGLDYHFGLILVAFIVIFYTFFGGYLAVSITDFFQGVIMLIAMVMVPIVAM
                     MNLNGWGTFHDVAAMKPTNLNLFKGLSFIGIISLFSWGLGYFGQPHIIVRFMSIKSHK
                     MLPKARRLGISWMAVGLLGAVAVGLTGIAFVPAYHIKLEDPETLFIVMSQVLFHPLVG
                     GFLLAAILAAIMSTISSQLLVTSSSLTEDFYKLIRGEEKAKTHQKEFVMIGRLSVLVV
                     AIVAIAIAWNPNDTILNLVGNAWAGFGASFSPLVLFALYWKGLTRAGAVSGMVSGALV
                     VIVWIAWIKPLAHINEIFGLYEIIPGFIVSVIVTYVVSKLTKKPGAFVETDLNKVRDI
                     VREK"
     gene            8250..8552
                     /gene="gatC"
                     /locus_tag="NBI57_001109"
     CDS             8250..8552
                     /gene="gatC"
                     /locus_tag="NBI57_001109"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HCD1822069.1"
                     /translation="MTKVTREEVEHIANLARLQISPEETEEMANTLESILDFAKQNDS
                     ADTEGVEPTYHVLDLQNVLREDKAIKGIPQELALKNAKETEDGQFKVPTIMNEEDA"
     gene            8554..10011
                     /gene="gatA"
                     /locus_tag="NBI57_001110"
     CDS             8554..10011
                     /gene="gatA"
                     /locus_tag="NBI57_001110"
                     /EC_number="6.3.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HCD1822070.1"
                     /translation="MSIRYESVENLLTLIKDKKIKPSDVVKDIYDAIEETDPTIKSFL
                     ALDKENAIKKAQELDELQAKDQMDGKLFGIPMGIKDNIITNGLETTCASKMLEGFVPI
                     YESTVMEKLHKENAVLIGKLNMDEFAMGGSTETSYFKKTVNPFDHKAVPGGSSGGSAA
                     AVAAGLVPFSLGSDTGGSIRQPAAYCGVVGMKPTYGRVSRFGLVAFASSLDQIGPLTR
                     NVKDNAIVLEAISGADVNDSTSAPVDDVDFTSEIGKDIKGLKVALPKEYLGEGVADDV
                     KEAVQNAVETLKSLGAVVEEVSLPNTKFGIPSYYVIASSEASSNLSRFDGIRYGYHSK
                     EAHSLEELYKMSRSEGFGKEVKRRIFLGTFALSSGYYDAYYKKSQKVRTLIKNDFDKV
                     FENYDVVVGPTAPTTAFNLGEEIDDPLTMYANDLLTTPVNLAGLPGISVPCGQSNGRP
                     IGLQFIGKPFDEKTLYRVAYQYETQYNLHDVYEKL"
     gene            10024..11451
                     /gene="gatB"
                     /locus_tag="NBI57_001111"
     CDS             10024..11451
                     /gene="gatB"
                     /locus_tag="NBI57_001111"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HCD1822071.1"
                     /translation="MHFETVIGLEVHVELKTDSKMFSPSPAHFGAEPNSNTNVIDLAY
                     PGVLPVVNKRAVDWAMRAAMALNMEIATESKFDRKNYFYPDNPKAYQISQFDQPIGEN
                     GYIDIEVDGETKRIGITRLHMEEDAGKSTHKGEYSLVDLNRQGTPLIEIVSEPDIRSP
                     KEAYAYLEKLRSIIQYTGVSDVKMEEGSLRCDANISLRPYGQEKFGTKAELKNLNSFN
                     YVRKGLEYEEKRQEEELLNGGEIGQETRRFDESTGKTILMRVKEGSDDYRYFPEPDIV
                     PLYIDDAWKERVRQTIPELPDERKAKYVNELGLPAYDAHVLTLTKEMSDFFESTIEHG
                     ADVKLTSNWLMGGVNEYLNKNQVELLDTKLTPENLAGMIKLIEDGTMSSKIAKKVFPE
                     LAAKGGNAKQIMEDNGLVQISDEATLLKFVNEALDNNEQSVEDYKNGKGKAMGFLVGQ
                     IMKASKGQANPQLVNQLLKQELDKR"
     gene            11491..11637
                     /locus_tag="NBI57_001112"
     CDS             11491..11637
                     /locus_tag="NBI57_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1822072.1"
                     /translation="MIADLDAIEGLFISIEVILLLTLFIVHVNGKNINFINALALIIL
                     RQTV"
     gene            12105..13052
                     /locus_tag="NBI57_001113"
     CDS             12105..13052
                     /locus_tag="NBI57_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HCD1822073.1"
                     /translation="MRKRARIIYNPTSGKELFKRELPDALIKLEKAGYETSAYATEKI
                     GDATLEAERAMHENYDVLIAAGGDGTLNEVVNGIAEKPNRPKLGVIPMGTVNDFGRAL
                     HIPNDIMGALDVIIEGHSTKVDIGKMNNRYFINLAAGGQLTQVSYETPSKLKSIVGPF
                     AYYIKGFEMLPQMKAVDLRIEYDGNVFQGEALLFFLGLTNSMAGFEKLVPDAKLDDGY
                     FTLIIVEKSNLAELGHIMTLASRGEHTKHPKVIYEKAKAINISSFTDLQLNVDGEYGG
                     KLPANFLNLERHIDVFAPNDIVNEELINNDHVDDNLIEE"
     gene            13133..14494
                     /gene="rlmD"
                     /locus_tag="NBI57_001114"
     CDS             13133..14494
                     /gene="rlmD"
                     /locus_tag="NBI57_001114"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HCD1822074.1"
                     /translation="MQAIAKNDIKTGTVVDLTHEGHGVVKIDRFPIFIPQALINEQIE
                     YKIIKVKKNFAIGKLLNINTRSENRVAPPCIYYERCGGCQLQHLSYEAQLEMKKEQVI
                     NLFQRKAHFDNSKINDTVGMTDPWRYRNKSQIPVGKNEQNEVIMGFYRQRSHDIIDME
                     SCLIQDSQHQEVMNEVKSILKDLNVSIYQEQLKKGLMRHLVVRTGYHTDEMMIIFVTN
                     GKKWPQKNAVVEKILDAFPNVTSIKQNINDSHSNVIMGRQSITLYGKDTIIDQLTDST
                     FKISDQSFYQINSEQTEKLYNKAIEYAQLTGNEVVLDTYCGIGTIGLYMAPHAKHVYG
                     VEVVPSSIEDAQQNATINQCNNTTFVCGKAEEVILQWKAQGIKPDVVMVDPPRKGCDE
                     TFIQTLLTLEPKRIVYISCNPATQQRDALLLAEKYQLEEVTPVDMFPQTTHVETVALF
                     NLK"
     gene            14664..>15117
                     /locus_tag="NBI57_001115"
     CDS             14664..>15117
                     /locus_tag="NBI57_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3267 domain-containing protein"
                     /protein_id="HCD1822075.1"
                     /translation="MHKIDLTTNNFQMRRFIILQLVIALFVILFTYKWALGVTAVVDQ
                     NIIINLVYGFAGFIILLILHELIHRALFLLFKKDSKPMFNIKKDRILFQTADACFNKW
                     QFSIIMLSPLILLSTGLLILIKVFGYSSLIFMFSMHTAYCFIDILLVAL"
BASE COUNT         5638 a         2207 c         2798 g         4474 t
ORIGIN      
        1 aatggttatc tatctgcact aataaggtag ataatcattg ttttttcacg aaaaaattta
       61 cagagtaaaa gaacttaaaa ttcatattaa gtctttagaa ctcgacactt aaaaatgcta
      121 taatcatatg tatgttaaaa aaggagtttc ggaaaatgta tgacattaaa aaatggcgcc
      181 atatttttaa attagaccca gctaaacata tttcagatga tgatttagat gcgatttgta
      241 tgtctcaaac agatgcaatt atgattggtg gaactgatga cgttactgaa gataatgtca
      301 ttcatttaat gagcagaata agaagatacc cattgccttt agtacttgaa atatcaaata
      361 tcgaaagtgt aatgcctggt tttgattttt attttgtacc tacagtactg aacagtacag
      421 atgttgcatt tcacaatggt acattattag aagcgcttaa aacatatgga catagtatag
      481 attttgaaga agtaatattt gaagggtatg tcgtgtgcaa tgctgatagc aaagtggcaa
      541 aacataccaa agcaaataca gatttaacaa cagaagattt agaagcatat gcccaaatgg
      601 tcaatcatat gtatcgatta ccggttatgt atatagagta tagtggcatt tatggcgacg
      661 tatcaaaggt tcaagctgtc tcagaacatc taacagaaac gcaacttttt tatggtggcg
      721 gtatttcctc agaacaacaa gcgacagaga tggcagctat tgcagataca attatcgtcg
      781 gtgatattat ttataaagat attaaaaaag ctttaaaaac agtaaaaata aaggagtcta
      841 gtaaatgaat gcgttattaa atcatatgaa tacagagcaa agtgaagctg taaagacaac
      901 agaaggacca ttgttaatta tggcaggtgc tggttcaggg aagacacgtg ttttaacaca
      961 tagaattgct tatttattag acgaaaaaga tgtctcacca tacaatgttt tggctattac
     1021 ttttacaaat aaagctgcaa gagaaatgaa agaacgtgtt caaaaattag taggtgatca
     1081 agcagaagtt atttggatgt caacattcca ctcaatgtgt gttcgtattt tacgtcgtga
     1141 tgcagatcga ataggtatag aacgcaattt tacgataatt gatcctacag accaaaaatc
     1201 tgttattaaa gacgtcttaa aaaatgaaaa tattgatagt aaaaagtttg aacctcgtat
     1261 gtttatcggt gcgatcagta atttgaaaaa tgaacttaaa acacctgcag atgctcaaaa
     1321 agaagccaca gattatcact cgcaaatggt agcaacggtt tatagtggat atcaacgcca
     1381 attgtcgcgt aatgaagcgt tagattttga tgaccttatt atgacaacga ttaacttatt
     1441 tgagcgtgta ccagaagttc tagaatatta tcagaacaaa ttccaatata ttcatgtaga
     1501 tgagtatcaa gatactaata aagcacaata tacattagtt aaattattag caagtaagtt
     1561 taaaaactta tgtgttgtag gtgactcaga tcagtcaatt tatggttggc gtggtgctga
     1621 tattcaaaat atcttatcat ttgaaaaaga ctacccagaa gcgaatacaa tctttttaga
     1681 gcaaaattat cgttcgacga aaacgatttt aaatgcggct aacgaagtga ttaaaaataa
     1741 ttctgaacgt aagccaaaag gactgtggac tgcaaatacg aatggtgaga aaattcatta
     1801 ctatgaagca atgacagaac gtgatgaagc ggaatttgta atacgagaaa ttatgaagca
     1861 tcaacgtaat ggtaagaaat atcaagatat ggcaatttta tatagaacaa atgcacaatc
     1921 acgtgtactt gaggaaacat tcatgaaatc taatatgcca tacacaatgg ttggtggcca
     1981 aaagttctat gaccgtaaag aaatcaaaga tttattaagt tatttgcgta taattgccaa
     2041 tagtaatgat gacattagtt tgcaacgtat tattaatgtt ccaaaaagag gtgtaggtcc
     2101 ttcatctgtt gaaaaagttc aaaactatgc acttcaaaac aatatcagta tgtttgatgc
     2161 acttggagaa gctgatttta tcggattgtc aaaaaaggtg acacaagagt gtcttaattt
     2221 ttacgagtta atccaaagct tgataaaaga acaagaattt ttagaaattc atgaaatagt
     2281 tgatgaagta ttacaaaagt ctggctaccg agaaatgctt gaacgtgaaa atacactaga
     2341 atctcgaagt agattagaaa acatcgatga atttatgtca gtaccaaaag actatgagga
     2401 aaatacccca ttagaagaac agtcattaat taacttttta acggatttat cgttagtagc
     2461 tgatattgat gaggcagata ctgaaaatgg cgtaacacta atgacgatgc actcggctaa
     2521 gggccttgaa tttccaatcg tctttataat ggggatggaa gaatctttat tcccacatat
     2581 tagagcgatt aagagtgaag atgatcacga aatgcaagaa gaacgtcgta tttgttatgt
     2641 agcaattaca agggctgaag aggtgttata tatcactcat gcgacatcaa gaatgttatt
     2701 tggtcgccct cagtcgaata tgccatctag atttttaaag gaaattccag aatcactatt
     2761 agaaaatcat tcaagtggca aacgacaaac gatacaacct aaggcaaaac cttttgctaa
     2821 acgcggattt agtcaacgaa caacgtcaac gaaaaaacaa gtatcgtcat ctgattggaa
     2881 tgtaggtgac aaagtgatgc ataaagcctg gggagaaggc atggtgagta atgtaaacga
     2941 gaaaaatggc tcaatcgaac tagatattat ctttaaatca caagggccaa aacgtttgtt
     3001 agcgcaattt gcaccaattg aaaaaaagga ggattaaggg atggctgatt tatcgtctcg
     3061 tgtgaacgag ttacatgatt tattaaatca atacagttat gaatactatg tagaggataa
     3121 tccatctgta ccagatagtg aatatgacaa attacttcat gaactgatta aaatagaaga
     3181 ggagcatcct gagtataaga ctgtagattc tccaacagtt agagttggcg gtgaagccca
     3241 agcctctttc aaaaaagtca accatgacac gccaatgtta agtttaggga atgcatttaa
     3301 tgaggatgat ttgagaaaat tcgaccaacg catacgtgaa caaattggca acgttgaata
     3361 tatgtgcgaa ttaaaaattg atggcttagc agtatcattg aaatatgttg atggatactt
     3421 cgttcaaggt ttaacacgtg gtgatggaac aacaggtgaa gatattaccg aaaatttaaa
     3481 aacaattcat gcgatacctt tgaaaatgaa agaaccatta aatgtagaag ttcgtggtga
     3541 agcatatatg ccgagacgtt catttttacg attaaatgaa gaaaaagaaa aaaatgatga
     3601 gcagttattt gcaaatccaa gaaacgctgc tgcgggatca ttaagacagt tagattctaa
     3661 attaacggca aaacgaaagc taagcgtatt tatatatagt gtcaatgatt tcactgattt
     3721 caatgcgcgt tcgcaaagtg aagcattaga tgagttagat aaattaggtt ttacaacgaa
     3781 taaaaataga gcgcgtgtaa ataatatcga tggtgtttta gagtatattg aaaaatggac
     3841 aagccaaaga gagtcattac cttatgatat tgatgggatt gttattaagg ttaatgattt
     3901 agatcaacag gatgagatgg gattcacaca aaaatctcct agatgggcca ttgcttataa
     3961 atttccagct gaggaagtag taactaaatt attagatatt gaattaagta ttggacgaac
     4021 aggtgtagtc acacctactg ctattttaga accagtaaaa gtagctggta caactgtatc
     4081 aagagcatct ttgcacaatg aggatttaat tcatgacaga gatattcgaa ttggtgatag
     4141 tgttgtagtg aaaaaagcag gtgacatcat acctgaagtt gtacgtagta ttccagaacg
     4201 tagacctgag gatgctgtca catatcatat gccaacccat tgtccaagtt gtggacatga
     4261 attagtacgt attgaaggcg aagtagcact tcgttgcatt aatccaaaat gccaagcaca
     4321 acttgttgaa ggattaattc actttgtatc aagacaagcc atgaatattg atggtttagg
     4381 cactaaaatt attcaacagc tttatcaaag cgaattaatt aaagatgttg ctgatatttt
     4441 ctatttaaca gaagaagatt tattaccttt agatagaatg gggcagaaaa aagttgataa
     4501 tttattagct gccattcaac aagctaagga caactcttta gaaaatttat tatttggtct
     4561 aggtattagg catttaggtg ttaaagcgag ccaagtgtta gcagaaaaat atgaaacgat
     4621 agatcgatta ctaacggtaa ctgaagcgga attagtagaa attcatgata taggtgataa
     4681 agtagcacaa tctgtagtta cttatttaga aaatgaagat attcgtgctt taattcaaaa
     4741 attaaaagat aaacatgtta atatgattta taaaggtatc aaaacatcag atattgaagg
     4801 acatcctgaa tttagtggta aaacgatagt actgactggt aagctacatc aaatgacacg
     4861 caatgaagca tctaaatggc ttgcatcaca aggtgctaaa gttacaagta gcgttactaa
     4921 aaatacagat gtcgttattg ctggtgaaga tgcaggttca aaattaacaa aagcacaaag
     4981 tttaggtatt gaaatttgga cagagcaaca atttgtagat aagcaaaatg aattaaatag
     5041 ttagaggggt atgtcgatga agcgtacatt agtattattg attacagcta tctttatact
     5101 cgctgcttgt ggtaaccata aggatgacca ggctggaaaa gataatcaaa aacataacaa
     5161 tagttcaaat caagtaaaag aaattgctac ggataaaaat gtacaaggtg ataactatcg
     5221 tacattgtta ccatttaaag aaagccaggc aagaggactt ttacaagata acatggcaaa
     5281 tagttataat ggcggcgact ttgaagatgg tttattgaac ttaagtaaag aagtatttcc
     5341 aacagataaa tatttgtatc aagatggtca atttttggac aagaaaacaa ttaatgccta
     5401 tttaaatcct aagtatacaa aacgtgaaat cgataagatg tctgaaaaag ataaaaaaga
     5461 caagaaagcg aatgaaaatt taggacttaa tccatcacac gaaggtgaaa caaatcctga
     5521 aaagattgca gaaaaatcac cagcctattt atctaacatt ttagagcaag atttttatgg
     5581 tggtggagat acaaaaggta agaatattaa aggtatgacg attggtttag ctatgaatag
     5641 tgtttattac tataaaaaag aaaaagatgg accgactttt agtaaaaaac tagatgatag
     5701 cgaagttaaa aagcaaggta aacaaatggc tagtgagata ttatcaaggt tacgtgaaaa
     5761 tgatgattta aaagatatac caattcattt tgcaatttat aagcaatcaa gtgaagattc
     5821 aatcacacca ggtgaattta tcactcaagc gactgcagaa aagagtcaaa caaagcttaa
     5881 tgaatggcat aatatcaatg aaaaatcagc tttattacct tcttcaacag cagcagatta
     5941 tgatgaaaat ttaaataata atttcaagca atttaatgat aatttgcaat catatttttc
     6001 taatttcaca caagcagtag ggaaagttaa atttgttgat aaaaagccac aacgattagt
     6061 agtagattta ccaatcgatt actatggaca agctgaaaca attggtatta cacagtacgt
     6121 tactgaacaa gcgaataaat atttcgataa aatcgataac tatgaaattc gtattaaaga
     6181 tggtaaccaa ccacgtgctt taattagtaa gacaaaagat gacaaagaac cgcaagttca
     6241 tatttacagt aattaattgt aaattaaaga taataaaaag gtaccaatgt tagtcataat
     6301 tttgattaac attagtacct ttttaatttg ttaagactta tgaattattt ttctctaacg
     6361 atgtcacgaa ctttgtttaa gtcagtttca acaaatgcac caggtttttt agtaagttta
     6421 cttacaacat atgtaacgat tacacttaca ataaatccag gaataatttc atataagccg
     6481 aatatttcgt tgatatgtgc caatggttta atccacgcaa tccaaacgat aacgactaag
     6541 gcacctgaaa ccattccact tacagcaccg gcacgtgtca aacctttcca gtaaagtgca
     6601 aatagcacaa gtggactgaa cgatgcacca aatccggccc aagcgttacc tactaagttt
     6661 agaattgtgt cgtttggatt ccatgcaatc gcgatggcaa caattgctac aactaataca
     6721 gataatcttc caatcataac aaattctttt tggtgcgttt tagctttttc ttcaccacga
     6781 attaatttat aaaagtcttc cgttagtgaa ctagatgtta caagtaattg tgaagaaatc
     6841 gtgctcataa ttgcagctag aatcgcagca agtaagaaac cacctacaag aggatggaag
     6901 agtacttgac tcatcacgat gaataatgtc tcaggatctt ctagtttaat atgataagca
     6961 ggtacgaatg caatacctgt taaaccaaca gccacagcgc ctaataaacc aacagccatc
     7021 cagctaatac ctaaacgtct agctttaggt agcatcttgt gtgatttaat agacataaac
     7081 cttacaatga tatgaggttg accgaaataa cctaatcccc atgaaaatag agagataatt
     7141 cctataaatg ataacccttt aaataaattt aaatttgtag gtttcatagc tgctacatca
     7201 tgaaacgttc cccagccgtt taaattcatc atagcaacaa tagggaccat aaccatcgca
     7261 attaacataa tgacaccttg gaagaaatct gtaattgata cagctaaata tccaccaaag
     7321 aaagtataga aaatgacaat gaaagcaact aatattaaac cgaaatgata atctaatcca
     7381 aaagcacttt caaataattt accaccagat acgaaaccag aatgtgtata taatgtaaag
     7441 aatactacga taatcaatcc agaaataatc tttaacacat tatttttatc gtttaaacga
     7501 tttttaaaga aatctggtaa tgtaattgca tctccagcta attcggtata aacacgaagt
     7561 ctaggagcaa caacaaagta atttatataa gcacctaatg ttaaaccgat tgtaatccac
     7621 atagctgata gaccagtgct atagacagaa ccaggtagcc ccataatcat ccatccactc
     7681 atatctgaag ctccagctga taatgcagta atatacggtc caatactacg tccacctaac
     7741 atgtactcgc ttaggttacc agttgcttgc ttgtaaccgt aaaagccaat aacgattagt
     7801 atcaagaagt agacggcaat cataatataa gtttgccaat tggcatctac ttgttgactt
     7861 aatgctgtcc ccattgtaag catgataaac actcccttta tttaatttat aatttagtta
     7921 cttgaaaaca ttatacataa agataaatgt ttagaaaagt aggaaaatta aatttattct
     7981 ttttgaaata cttgcatacc aatataggaa gcgctttcgt attttttgga tatgagcaaa
     8041 tttttataat taaaattgca taatcataat attgaattgg gttgtctaat attaaaagag
     8101 tgttgttttc ataaaaaatg atttgataaa cgaatgataa tgtttataca gtttatatga
     8161 aattaaagta gcacctcata aatacttaga tttttaattg gaaatttgat acaatttagt
     8221 gatgaatgac ttaaaggagg cttttattaa tgacaaaagt aacacgtgaa gaagttgagc
     8281 atatcgcgaa tcttgcaaga cttcaaattt ctcctgaaga aacggaagaa atggccaaca
     8341 cattagaaag cattttagat tttgcaaaac aaaatgatag cgctgataca gaaggcgttg
     8401 aacctacata tcacgtttta gatttacaaa acgttttacg tgaagataaa gcaattaaag
     8461 gtattccgca agaattagct ttgaaaaatg ccaaagaaac agaagatgga caatttaaag
     8521 tgcctacaat catgaatgag gaggacgcgt aagatgagca ttcgctacga atcggttgag
     8581 aatttattaa ctttaataaa agacaaaaaa atcaaaccat ctgatgttgt taaagatata
     8641 tatgatgcaa ttgaagagac tgatccaaca attaagtctt ttctagcgct ggataaagaa
     8701 aatgcaatca aaaaagcgca agaattggat gaattacaag caaaagatca aatggatggc
     8761 aaattatttg gtattccaat gggtataaaa gataacatta ttacaaacgg attagaaaca
     8821 acatgtgcaa gtaaaatgtt agaaggtttt gtgccaattt acgaatctac tgtaatggaa
     8881 aaactacata aagagaatgc cgttttaatc ggtaaattaa atatggatga gtttgcaatg
     8941 ggtggttcaa cagaaacatc ttatttcaaa aaaacagtta acccatttga ccataaagca
     9001 gtaccaggtg gttcatcagg tggatctgca gcagcagttg cagctggctt agtaccattt
     9061 agcttaggtt cagacacagg tggttcaatt agacaaccgg ctgcatattg tggcgttgtc
     9121 ggtatgaaac caacatacgg tcgtgtatct cgatttggat tagttgcttt tgcatcttca
     9181 ttagaccaaa ttggtccatt gactcgaaat gtaaaagata atgcaatcgt attagaagct
     9241 atttctggtg cagatgttaa tgactctaca agtgcaccag ttgatgatgt agactttaca
     9301 tctgaaattg gtaaagatat taaaggatta aaagttgcat tacctaaaga atacttaggt
     9361 gaaggtgtag ctgatgacgt aaaagaagca gttcaaaacg ctgtagaaac tttaaaatct
     9421 ttaggtgctg tcgttgagga agtatcattg ccaaatacta aatttggtat tccatcatat
     9481 tacgtgattg catcatcaga agcttcgtca aacctttctc gttttgacgg aattcgttat
     9541 ggttatcatt ctaaagaagc tcattcatta gaagaattat ataaaatgtc aagatctgaa
     9601 ggtttcggta aagaagtaaa acgtcgtatt ttcttaggta catttgcatt aagttcaggt
     9661 tactacgatg cttactataa aaaatctcaa aaagttagaa cattgattaa aaatgacttt
     9721 gataaagtat tcgaaaatta tgatgtagta gttggtccaa cagcgcctac aactgcgttt
     9781 aatttaggtg aagaaattga tgatccatta acaatgtatg ccaatgattt attaacaaca
     9841 ccagtaaact tagctggatt acctggtatt tctgttcctt gtggacaatc aaatggccga
     9901 ccaatcggtt tacagttcat tggtaaacca ttcgatgaaa aaacgttata tcgtgtcgct
     9961 tatcaatatg aaacacaata caatttacat gacgtttatg aaaaattata aggagtggaa
    10021 atcatgcatt ttgaaacagt tataggactt gaagttcacg tagagttaaa aacggactca
    10081 aaaatgtttt ctccatcacc agcgcatttt ggagcagaac ctaactcaaa tacaaatgtt
    10141 atcgacttag catatccagg tgtcttacca gttgttaata agcgtgcagt agactgggca
    10201 atgcgtgctg caatggcact aaatatggaa atcgcaacag aatctaagtt tgaccgtaag
    10261 aactatttct atccagataa tccaaaagca tatcaaattt ctcaatttga tcaaccaatt
    10321 ggtgaaaatg gatatatcga tatcgaagtc gacggtgaaa caaaacgaat cggtattact
    10381 cgtcttcaca tggaagaaga tgctggtaag tcaacacata aaggtgagta ttcattagtt
    10441 gacttgaacc gtcaaggtac accgctaatt gaaatcgtat ctgaaccaga tattcgttca
    10501 cctaaagaag catatgcata tttagaaaaa ttacgttcaa ttattcaata cactggtgta
    10561 tcagacgtta agatggaaga gggatcttta cgttgtgatg ctaacatctc tttgcgtcca
    10621 tatggtcaag aaaaatttgg tactaaagcc gaattgaaaa acttaaactc atttaactat
    10681 gtacgtaaag gtttagaata tgaagaaaaa cgccaagaag aagaattgtt aaatggtgga
    10741 gaaatcggac aagaaacacg tcgatttgat gaatctacag gtaaaacaat tttaatgcgt
    10801 gttaaagaag gttctgatga ttaccgttac ttcccagagc ctgacattgt acctttatat
    10861 attgatgatg cttggaaaga gcgtgttcgt cagacaattc ctgaattacc agatgagcgt
    10921 aaggctaagt atgtaaatga attaggttta cctgcatacg atgcacacgt attaacattg
    10981 actaaagaaa tgtcagattt ctttgaatca acaattgaac acggtgcaga tgttaaatta
    11041 acatctaact ggttaatggg tggcgtaaac gaatatttaa ataaaaatca agtagaatta
    11101 ttagatacta aattaacacc agaaaattta gcaggtatga ttaaacttat cgaagacgga
    11161 acaatgagca gtaaaattgc gaagaaagtc ttcccagagt tagcagctaa aggtggtaat
    11221 gctaaacaga ttatggaaga taatggctta gttcaaattt ctgatgaagc aacacttcta
    11281 aaatttgtaa atgaagcatt agacaataac gaacaatcag ttgaagatta caaaaatggt
    11341 aaaggcaaag ctatgggctt cttagttggt caaattatga aagcgtctaa aggtcaagct
    11401 aatccacaat tagtaaatca actattaaaa caagaattag ataaaagata atttaaatca
    11461 tcaaactatg aagatttaaa aaataaaccc ttgattgctg acttagatgc aatcgagggt
    11521 ttatttatat ctatagaagt catattactt ttaactttat tcattgtaca tgttaatggt
    11581 aaaaatatta attttattaa tgcgttagct ttaattatat taaggcaaac tgtataataa
    11641 aaaggtataa aacatttttg tataaagaca acattatatt tacaacatca ttttaaaggt
    11701 aaaatagcat aactgacgaa gtctatataa tgaagaacgg caaaaaatgc tgaataaata
    11761 acaagctttg tacatattga gatagtattt gtttaagata caagttggtc tttaacgata
    11821 ttaagaatga tgaaataaga ctgagcctgg gtcataaatt caatgtccta ggcactacaa
    11881 tgttatattg gcagtagttg actgaaagaa aatacgcttg taacaagctt ttttcaattc
    11941 tagtggggcc ccaaacaaag agaatttcga aaggaaattc tacagacaat gcaagttggg
    12001 gtagaacgaa ataaattttg ttaaatatta tttctgtccc actccctatt agacgaaaca
    12061 aagatgaagt taaaatatat gaattttaag tagagggata agatatgagg aaacgtgcta
    12121 gaatcattta taacccgaca tcaggtaaag agctatttaa aagagaatta cctgatgcct
    12181 taataaaatt agaaaaagcg ggatatgaaa cgagtgcata tgcaaccgag aaaataggtg
    12241 atgccacact tgaagcagaa agagctatgc atgaaaatta tgatgtatta atcgctgcag
    12301 gtggtgatgg aacattaaat gaagtagtta atggtatcgc agaaaagcct aatcgtccta
    12361 agctaggtgt cattcctatg ggtactgtta atgactttgg acgtgcattg catataccta
    12421 atgacatcat gggggcactt gatgtcatca ttgaaggtca ttctactaaa gtagatattg
    12481 gtaaaatgaa taatcgatac tttattaatt tagctgcagg cggacaattg acgcaagtct
    12541 cttatgaaac accgagtaaa ttgaaatcta ttgttggtcc atttgcttat tacatcaaag
    12601 gtttcgaaat gttacctcaa atgaaagctg tagatttaag aattgaatat gatggtaatg
    12661 ttttccaagg agaagcatta ttattctttt taggtttaac aaattcaatg gcaggatttg
    12721 aaaaattagt gccggacgct aagttagatg acggctattt tacgttaatt atagttgaaa
    12781 aatcaaacct tgcagaactt ggtcatatta tgactttagc ttcaagagga gaacatacta
    12841 agcatcctaa agttatttat gaaaaagcaa aggcaattaa tatttcatca ttcacagact
    12901 tacaactaaa tgtagatgga gaatatggtg gtaaattacc agcgaatttc ttaaatctag
    12961 aacgtcatat agatgtcttt gcacctaatg atattgtaaa tgaagagtta ataaataatg
    13021 atcatgttga tgacaattta atcgaagaat aaacagaaat agtgaaatat ataatggcag
    13081 gttaggaagc gcataatttt gttcctaacc tgttcatttt ggaggtaaaa ttttgcaagc
    13141 aatagctaaa aatgacataa aaacgggaac agttgttgat ttaacgcatg aaggccatgg
    13201 tgttgtaaaa atagatcgct ttcctatttt cataccacaa gctttaatta atgaacaaat
    13261 tgaatataaa attatcaaag taaaaaagaa ctttgcgata ggtaagttgc ttaatataaa
    13321 tacgagaagt gagaatcgtg ttgcaccgcc atgtatatat tatgaacgtt gtggtgggtg
    13381 ccaacttcaa cacttatcat acgaagcaca acttgaaatg aaaaaggaac aagtgattaa
    13441 tttgtttcag cgaaaagcgc attttgataa ttctaagatt aacgatacag ttggcatgac
    13501 agatccttgg agatatcgaa acaagtccca gattccagtt ggtaagaatg aacagaatga
    13561 agttattatg ggcttttatc gtcaaagaag ccatgacatt attgacatgg aaagttgttt
    13621 aatacaagat tcacaacatc aagaagtgat gaatgaagtt aaatctattt taaaagattt
    13681 aaatgtaagt atttatcaag aacaattgaa aaaaggttta atgagacatt tggttgtaag
    13741 aacaggatat catacagacg aaatgatgat tatttttgta actaatggta aaaagtggcc
    13801 acaaaaaaat gctgttgttg agaagatatt agatgcattt cctaatgtga caagcattaa
    13861 acaaaacatt aatgatagcc actctaatgt tattatggga cgtcaatcta taacgctata
    13921 tggtaaagat acaattattg atcaattaac agatagtaca tttaaaataa gtgatcagtc
    13981 attctaccaa attaattctg aacaaacaga gaaattatat aataaagcaa ttgagtatgc
    14041 gcaattgaca ggtaatgaag ttgtattaga tacctattgt ggtattggaa caatagggct
    14101 atatatggca ccacatgcaa aacatgtata cggtgttgaa gtagtacctt cttcaattga
    14161 agacgcacaa caaaatgcga ctataaacca atgcaataat acaacttttg tctgtgggaa
    14221 agctgaagaa gttattttac aatggaaagc acaagggatt aaaccagatg ttgtaatggt
    14281 tgatccgcca agaaagggct gtgacgaaac gtttattcag actttgctta cattagaacc
    14341 taagcgtatt gtctatattt catgtaatcc cgcaacacag caacgtgatg cattattatt
    14401 agctgaaaaa tatcaacttg aagaggtaac accggtagat atgtttccac aaacaacaca
    14461 tgttgagaca gtggcattat tcaatttgaa atagcgcaca tgatttgaga taaaatttcg
    14521 ccttaataag tgaagaaaga agtcgtatat taaaatttta ttacagccaa tttactttct
    14581 tgatatgaac tttttaaatt aaatgtgaca ttgtatacta tatttaaaga agaataagaa
    14641 tgtcatgatc gggagggttg gtaatgcata aaattgattt aacgacaaat aattttcaaa
    14701 tgcgaaggtt tattatcttg caacttgtta ttgcgttatt cgtgatttta tttacgtata
    14761 agtgggcgtt aggtgtcaca gcggttgtcg atcaaaatat catcatcaat cttgtatatg
    14821 ggtttgctgg attcattatt ttattgattt tacatgaact tatccataga gcattatttc
    14881 ttttatttaa aaaagatagc aaacctatgt ttaatataaa aaaagacaga atcttattcc
    14941 aaacagcaga tgcttgcttc aacaaatggc aattctcaat tattatgctg tcaccactaa
    15001 tcttattaag tactggcttg cttattttaa ttaaggtatt tggttattca tcacttattt
    15061 ttatgtttag tatgcatacc gcttattgct ttatagatat tctgcttgtt gcattaa
//
LOCUS       DAHHSC010000048        14821 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.048, whole genome shotgun sequence.
ACCESSION   DAHHSC010000048 DAHHSC010000000
VERSION     DAHHSC010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 14821)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14821)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14821
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.048"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            complement(147..1145)
                     /gene="sirC"
                     /locus_tag="NBI57_001116"
     CDS             complement(147..1145)
                     /gene="sirC"
                     /locus_tag="NBI57_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphyloferrin B ABC transporter permease
                     subunit SirC"
                     /protein_id="HCD1822076.1"
                     /translation="MTEKINKKDNYHLIFALIFLAIVSVVSMMIGSSFIPLQRVLMYF
                     INPNDSMDQFTLEVLRLPRITLAILAGAALGMSGLMLQNVLKNPIASPDIIGITGGAS
                     LSAVVFIAFFSHLTIHLLPLFAVLGGAVAMMILLVFQTKGQIRPTILIIIGISMQTLF
                     IALVQGLLITTKQLSAAKAYTWLVGSLYGATFKDTIILGMVILAVVPLLFLVIPKMKI
                     SILDDPVAIGLGLHVQRMKLIQLITSTILVSMAISLVGNIGFVGLIAPHIAKTIVRGS
                     YAKKLLMSAMIGAISIVIADLIGRTLFLPKEVPAGVFIAAFGAPFFIYLLLTVKKL"
     gene            complement(1142..2137)
                     /gene="sirB"
                     /locus_tag="NBI57_001117"
     CDS             complement(1142..2137)
                     /gene="sirB"
                     /locus_tag="NBI57_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphyloferrin B ABC transporter permease
                     subunit SirB"
                     /protein_id="HCD1822077.1"
                     /translation="MLLKPKYQIVIAGLCLAIVAILSLMIGNTLVSPGTVIQALFNFD
                     SENDLHDVVTGARASRTIIALLTGAALAVSGLLMQALTRNPIASPGLFGVNAGAVFFV
                     IFSITFIQIQSFKMIVVIAFLGAIVVTILVVALGMFRQTLFSPHRVILAGAAIAMLFT
                     AFTQGILIMNETDLQGLLFWLSGSVSLRNIWDIPWIIPLVLILILIAFSMAAHINILM
                     TSDDIATGLGQNIKLIKWMIIMLISMLAGISVAVAGSIVFVGLIVPNISKRLLPPNYK
                     YLIPFTALAGAILMIISDIVARIIIKPLELPIGVVTAVIGAIVLIYIMKKGRQRL"
     gene            complement(2153..3145)
                     /gene="sirA"
                     /locus_tag="NBI57_001118"
     CDS             complement(2153..3145)
                     /gene="sirA"
                     /locus_tag="NBI57_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphyloferrin B ABC transporter
                     substrate-binding protein SirA"
                     /protein_id="HCD1822078.1"
                     /translation="MNKVIKMLVVTLAFLLVLAGCSGNSNKQSSDNKDKETTSIKHAM
                     GTTEIKGKPKRVVTLYQGATDVAVSLGVKPVGAVESWTQKPKFEYIKNDLKDTKIVGQ
                     EPAPNLEEISKLKPDLIVASKVRNEKVYDQLSKIAPTVSTDTVFKFKDTTKLMGKALG
                     KEKEAEDLLKKYDDKVAAFQKDAKAKYKDAWPLKASVVNFRADHTRIYAGGYAGEILN
                     DLGFKRNKDLQKQVDNGKDIIQLTSKESIPLMNADHIFVVKSDPNAKDAALVKKTESE
                     WTSSKEWKNLDAVKNNQVSDDLDEITWNLAGGYKSSLKLIDDLYEKLNIEKQSK"
     gene            3376..4356
                     /gene="sbnA"
                     /locus_tag="NBI57_001119"
     CDS             3376..4356
                     /gene="sbnA"
                     /locus_tag="NBI57_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000570808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diaminopropionate biosynthesis protein SbnA"
                     /protein_id="HCD1822079.1"
                     /translation="MIEKSQACHDSLLDSVGQTPMVQLHQLFPKHEVFAKLEYMNPGG
                     SMKDRPAKYIIEHGIKHGLITENTHLIESTSGNLGIALAMIAKIKGLKLTCVVDPKIS
                     PTNLKIIKSYGANVEMVEEPDAHGGYLMTRIAKVQELLATIDDAYWINQYANELNWQS
                     HYHGAGTEIVETIKQPIDYFVAPVSTTGSIMGMSRKIKEVHPNAQIVAVDAKGSVIFG
                     DKPINRELPGIGASRVPEILNRSEINQVIHVDDYQSALGCRKLIDYEGIFAGGSTGSI
                     IAAIEQLITSIEEGATIVTILPDRGDRYLDLVYSDTWLEKMKSRQGVKSE"
     gene            4353..5363
                     /gene="sbnB"
                     /locus_tag="NBI57_001120"
     CDS             4353..5363
                     /gene="sbnB"
                     /locus_tag="NBI57_001120"
                     /EC_number="1.5.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-[(2S)-2-amino-2-carboxyethyl]-L-glutamate
                     dehydrogenase SbnB"
                     /protein_id="HCD1822080.1"
                     /translation="MNREMLYLNRSDIEQAGGNHSQVYVDALTEALTAHAHNDFVQPL
                     KPYLRQDPENGHIADRIIAMPSHIGGEHAISGIKWIGSKHDNPSKRNMERASGVIILN
                     DPETNYPIAVMEASLISSMRTAAVSVIAAKHLAKKGFKDLTIIGCGLIGDKQLQSMLE
                     QFDHIKRVFVYDQFSEACARFVDRWQQQRPEINFIATENAKEAVSNGEVVITCTVTDQ
                     PYIEYDWLQKGAFISNISIMDVHKEVFIKADKVVVDDWSQCNREKKTINQLVLEGKFS
                     KEALHAELGQLVTGDIPGREDDDEIILLNPMGMAIEDISSAYFIYQQAQQQNIGTTLN
                     LY"
     gene            5384..7138
                     /gene="sbnC"
                     /locus_tag="NBI57_001121"
     CDS             5384..7138
                     /gene="sbnC"
                     /locus_tag="NBI57_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphyloferrin B biosynthesis protein SbnC"
                     /protein_id="HCD1822081.1"
                     /translation="MQNHTAVNTAQAIILRDLVDALLFEDIAGIVSNSEITKENGQTL
                     LIYERETQQIKIPVYFSALNMFRYESSQPITIEGRVSKQPLTAAEFWQTIANMNCDLS
                     HEWEVARVEEGLTTAATQLAKQLSELDLASHPFVMSEQFASLKDRPFHPLAKEKRGLR
                     EADYQVYQAELNQSFPLMVAAVKKTHMIHGDTANIDELENLTVPIKEQATDMLNDQGL
                     SIDDYVLFPVHPWQYQHILPNVFATEISEKLVVLLPLKFGDYLSSSSMRSLIDIGAPY
                     NHVKVPFAMQSLGALRLTPTRYMKNGEQAEQLLRQLIEKDEALAKYVMVCDETAWWSY
                     MGQDNDIFKDQLGHLTVQLRKYPEVLAKNDTQQLVSMAALAANDRTLYQMICGKDNIS
                     KNDVMTLFEDIAQVFLKVTLSFMQYGALPELHGQNILLSFEDGRVQKCVLRDHDTVRI
                     YKPWLTAHQLSLPKYVVREDTPNTLINEDLETFFAYFQTLAVSVNLYAIIDAIQDLFG
                     VSEHELMSLLKQILKNEVATISWVTTDQLAVRHILFDKQTWPFKQILLPLLYQRDSGG
                     GSMPSGLTTVPNPMVTYD"
     gene            7131..8387
                     /gene="sbnD"
                     /locus_tag="NBI57_001122"
     CDS             7131..8387
                     /gene="sbnD"
                     /locus_tag="NBI57_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphyloferrin B export MFS transporter"
                     /protein_id="HCD1822082.1"
                     /translation="MINQSIWRSNFRILWLSQFIAIAGLTVLVPLLPIYMASLQNLSV
                     VEIQLWSGIAIAAPAVTTMIASPIWGKLGDKISRKWMVLRALLGLAVCLFLMALCTTP
                     LQFVLVRLLQGLFGGVVDASSAFASAEAPAEDRGKVLGRLQSSVSAGSLVGPLIGGVT
                     ASILGFSALLMSIAVITFIVCIFGALKLIETTHMPKSQTPNINKGIRRSFQCLLCTQQ
                     TCRFIIVGVLANFAMYGMLTALSPLASSVNHTAIDDRSVIGFLQSAFWTASILSAPLW
                     GRFNDKSYVKSVYIFATIACGCSAILQGLATNIEFLMAARILQGLTYSALIQSVMFVV
                     VNACHQQLKGTFVGTTNSMLVVGQIIGSLSGAAITSYTTPATTFIVMGVVFAVSSLFL
                     ICSTITNQINDHTLMKLWELKQKSAK"
     gene            8377..10113
                     /gene="sbnE"
                     /locus_tag="NBI57_001123"
     CDS             8377..10113
                     /gene="sbnE"
                     /locus_tag="NBI57_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-2,3-diaminopropanoate--citrate ligase SbnE"
                     /protein_id="HCD1822083.1"
                     /translation="MQNKELIQHAAYAAIERILNEYFREENLYQVPPQNHQWSIQLSE
                     LETLTGEFRYWSAMGHHMYHPEVWLIDGKSKKITTYKEAIARILQHMAQSADNQTAVQ
                     QHMAQIMSDIDNSIHRTARYLQSNTIDYVEDRYIVSEQSLYLGHPFHPTPKSASGFSE
                     ADLEKYAPECHTSFQLHYLAVHQDVLLTRYVEGKEDQVEKVLYQLADIDISEIPKDFI
                     LLPTHPYQINVLRQHPQYMQYSEQGLIKDLGVSGDLVYPTSSVRTVFSKALNIYLKLP
                     IHVKITNFIRTNDLEQIERTIDAAQVIASVKDEVETPHFKLMFEEGYRALLPNPLGQT
                     VEPEMDLLTNSAMIVREGIPNYHADKDIHVLASLFETMPDSPISKLAQVIEQSGLAPE
                     AWLECYLDRTLLPILKLFSNTGISLEAHVQNTLIELKDGIPDVCFVRDLEGICLSRTI
                     ATEKQLVPNVVAASSPVVYAHDEAWHRLKYYVVVNHLGHLVSTIGKATRNEVVLWQLV
                     AHRLMTWKKEYANNAVFVDCVEDLYQTPTIAAKANLMSKLNDCGANPIYTHIPNPICY
                     NKEVSYCESNNS"
     gene            10022..11872
                     /gene="sbnF"
                     /locus_tag="NBI57_001124"
     CDS             10022..11872
                     /gene="sbnF"
                     /locus_tag="NBI57_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-(L-alanin-3-ylcarbamoyl)-2-[(2-
                     aminoethylcarbamoyl)methyl]-2-hydroxypropanoate synthase
                     SbnF"
                     /protein_id="HCD1822084.1"
                     /translation="MIVVQTLFIHIYQIQFVITRRYRIVNQTILNRVKTRVMHQLVSS
                     LIYENIVVYKASYQDGVGHFTIEGHDSEYRFTAEKTHSFDRIRITSPIERVVGDEADT
                     TTDYTQLLREAVFTFPKNDEKLEQFIVELLQTELKDTQSMQYRESNPPATPETFNDYE
                     FYAMEGHQYHPSYKSRLGFTLSDNLKFGPDFVPNVKLQWLAIDKDKVETTVSRNVVVN
                     EMLRQQVGDKTYEHFVQQIEASGKHVNDVEMIPVHPWQFEHVIQVDLAEERLNGTVLW
                     LGESDELYHPQQSIRTMSPIDTTKYYLKVPISITNTSTKRVLAPHTIENAAQITDWLK
                     QIQQQDMYLKDELKTVFLGEVLGQSYLNTQLSPYKQTQVYGALGVIWRENIYHMLIDE
                     EDAIPFNALYASDKDGLPFIEKWIKQYGSEAWTKQFLAVAIRPMIHMLYYHGIAFESH
                     AQNMMLIHENGWPTRIALKDFHDGVRFKREHLSEAASHLTLKPMPEAHKKVNSNSFIE
                     TDDERLVRDFLHDAFFFINIAEIILFIEKQYGIDEQRQWQWVKDIIEAYQEAFPELNN
                     YQHFDLFEPTIQVEKLTTRRLLSDSELRIHHVTNPLGVGGINDATTISET"
     gene            11847..12623
                     /gene="sbnG"
                     /locus_tag="NBI57_001125"
     CDS             11847..12623
                     /gene="sbnG"
                     /locus_tag="NBI57_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphyloferrin B biosynthesis citrate synthase
                     SbnG"
                     /protein_id="HCD1822085.1"
                     /translation="MQQLSLKHRLNNGDSVYGIFNSIPDPLMIEVIAASGYDFVVIDT
                     EHVAINDETLAHLIRAAEAAHIIPIVRVTAVIDRDIIKVLDMGARGIIVPHVKDRETV
                     EHIVKLSRYYPQGLRSLNGGRMARFGRTPLLDAMEMANEHIMVIAMIEDVEGVMAIDD
                     IAQVEGLDMIVEGAADLSQSLGIPWQTRDDQVTSHVQHIFEVVNAHGKHFCALPREDE
                     DIAKWQAQGVQTFILGDDRGKIYRHLSASLATSKQKGDDG"
     gene            12623..13825
                     /gene="sbnH"
                     /locus_tag="NBI57_001126"
     CDS             12623..13825
                     /gene="sbnH"
                     /locus_tag="NBI57_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphyloferrin B biosynthesis decarboxylase
                     SbnH"
                     /protein_id="HCD1822086.1"
                     /translation="MRIVQPVIEQLKAQSHPVCHYIYDLVGLEHHLQHITSSLPSNCQ
                     MYYAMKANSERTILDTISQYVEGFEVASQGEIAKGLAFKPANHIIFGGPGKTDEELRY
                     AVSEGVQRIHVESMHELQRLNAILEDEDKTQHILLRVNLAGPFPNATLHMAGRPTQFG
                     ISEDEVDDVIEAALVMPNIHLDGFHFHSISNNLDSNLHVDVVKLYFKKAKSWSEKHRF
                     PLKHINLGGGIGVNYADLTSQFEWDNFVENFKTLIVEQEMEDVTLNFECGRFIVAHIG
                     YYVTEVLDIKKVHGAWYAILRGGTQQFRLPVSWQHNHPFEIYRYKDNPYSFEKVSISR
                     QDTTLVGQLCTPKDVFAREVQIDAISTGDVIVFKYAGAYGWSISHHDFLSHPHPEFIY
                     LTQTKEDE"
     gene            13829..14593
                     /gene="sbnI"
                     /locus_tag="NBI57_001127"
     CDS             13829..14593
                     /gene="sbnI"
                     /locus_tag="NBI57_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional transcriptional
                     regulator/O-phospho-L-serine synthase SbnI"
                     /protein_id="HCD1822087.1"
                     /translation="MNHIHEHLKLVPVDKIDLHETFEPLRLEKTKSSIEADDFIRHPI
                     LVTAMQHGRYMVIDGVHRYTSLKALGCKKVPVQEIHETQYSISTWQHKVPFGVWWETL
                     QQEHRLPWTTETRQEAPFITMCHGDTEQYLYTKDLGEAHFQVWEKVVASYSGCCSVER
                     IAQGTYPCLSQQDVLMKYQPLSYKEIEAVVHKGETVPAGVTRFNISGRCLNLQVPLAL
                     LKQDDDVEQLRNWKQFLADKFANMRCYTEKVYLVEQ"
BASE COUNT         4939 a         2420 c         2957 g         4505 t
ORIGIN      
        1 gtatataaag tattaatatg taattaagaa agtcgacgct tgttaacaat aacaacaact
       61 atatgtataa aaaaacctcg caactgttag ttaactgaat catttagcta acttcgttgt
      121 gaggtcattt tgttttaata atatcgttat aactttttca cggttaataa taagtatatg
      181 aagaatgggg caccaaaagc agcaataaat acacctgctg gcacttcttt aggcaagaat
      241 aaggtacgcc caattaagtc tgcaataaca attgatatgg caccaatcat tgctgacatt
      301 agtaactttt tagcataact tccgcgaacg attgttttcg cgatatgtgg tgcgattaaa
      361 ccgacaaacc caatgttacc tactaaactg attgccatag atacgagtat agtagaagtg
      421 attaattgga ttagtttcat acgttgtaca tgtaagccta agccaatcgc tacagggtca
      481 tcaagtatag atattttcat ttttggtata acaagaaata acaacggcac aacagctaaa
      541 ataaccatac ccaaaatgat tgtatcttta aacgtagcac cgtaaagact tccgactagc
      601 catgtataag ctttggcagc agataattgc ttcgttgtaa tgagtaatcc ttggacaagc
      661 gcaataaaca acgtttgcat cgaaataccg atgattatga gtattgtcgg gcgtatttgt
      721 cctttcgttt gaaacactaa tagtatcatc attgcaactg cgccacctaa tactgcaaat
      781 agtggaagta aatgtatcgt taaatggctg aaaaatgcaa taaagacaac agcacttaag
      841 ctagcaccac ctgtgatacc gataatatca ggtgaggcaa ttggattttt taatacattt
      901 tgcaacatta aaccactcat tcctagtgcg gcacctgcta aaatcgcaag tgtaatgcga
      961 ggtaagcgta atacttctaa agtgaattga tccatactgt catttggatt tataaagtac
     1021 atcagtacgc gttgtaatgg tataaagctt gaaccaatca tcatacttac cactgaaacg
     1081 atggctaaaa agattaacgc gaagatgaga tggtaattgt cttttttatt aatcttttcg
     1141 gtcataagcg ttgacgtcct ttcttcataa tatagattaa gacaatagcg ccaatgacag
     1201 cggtaacgac accgataggc aactctagtg gcttaattat tatacgagca acaatgtctg
     1261 aaatgatcat taggattgct ccagctaatg cagtaaaagg aattaaatac ttatagtttg
     1321 gtggtaataa tcgtttgcta atattcggta cgataagacc cacaaagacg attgatccag
     1381 ctacggctac cgaaataccg gctaacatac tgatgagcat aataatcatc catttgatta
     1441 attttatgtt ttgaccgagg ccggttgcaa tgtcgtcact tgtcatcaag atgttgatgt
     1501 gtgcagccat gctaaatgca attaaaataa gtatcaatac aagcggaata atccatggga
     1561 tatcccaaat attacgtaat gaaacggagc cacttaacca aaataatagg ccttgtaagt
     1621 ctgtttcgtt cataataagt atgccttgag taaaggctgt aaatagcatc gcaatcgcag
     1681 cacctgccaa aatgacacgg tgaggtgaga atagtgtttg tctaaacata cctagcgcaa
     1741 caactaatat agtaacaaca atggcaccta aaaatgcaat aactacaatc attttaaaag
     1801 attgaatttg gataaatgta atactaaaaa tgacaaaaaa tactgcgcct gcattgacac
     1861 cgaaaagccc tggtgaggct attgggtttc gtgtaagtgc ttgcatcaac aaacctgaga
     1921 cagcaagggc agcaccagtc aataacgcaa tgattgttct cgacgcccgt gcaccagtga
     1981 caacatcatg taaatcgttt tcactatcaa agttgaataa cgcctgtatc accgtacctg
     2041 gtgacacaag cgtatttcca atcattaaac ttaagatagc tactattgca agacataaac
     2101 cagcaataac gatttggtat tttggtttaa gtagcatcgt aaaactcctt aattattttg
     2161 attgtttttc aatatttaac ttttcatata aatcgtcaat aagttttaat gaagatttat
     2221 atccgccagc taagttccaa gtgatttcat ctaaatcatc agatacttgg ttgtttttaa
     2281 ctgcgtctaa atttttccac tctttacttg aagtccattc gctttcagtc tttttaacta
     2341 atgcagcatc tttcgcattt ggatctgatt ttactacaaa aatatgatca gcgttcatta
     2401 atggaatgct ttctttagat gtaagttgga taatatcttt accattatca acttgttttt
     2461 gtaagtcttt attacgtttg aatcctaaat catttaagat ttcaccagca tatccaccag
     2521 cataaattct tgtatgatca gcacggaagt taacaactga agctttcaat ggccatgcat
     2581 ctttatactt tgcttttgca tctttttgga atgcagctac tttatcatcg tactttttaa
     2641 gtaaatcttc agcttctttt tctttcccta aagctttccc cattaactta gttgtatctt
     2701 tgaatttgaa aactgtatca gtagaaactg ttggtgcgat tttagataat tgatcgtaaa
     2761 ctttttcatt tctaactttt gacgcgacaa ttaagtccgg ttttaattta gagatttcct
     2821 ctaagttagg tgcaggttct tgacctacaa tcttagtatc ttttaaatca ttttttatgt
     2881 attcgaattt cggtttttgt gtccatgatt ctacagcacc tacaggttta acacctaaag
     2941 atacagcgac gtcagtggca ccttgatata gcgtaacaac acgctttggt ttccctttaa
     3001 tttcagttgt acccattgca tgtttaattg aagttgtttc cttatctttg ttatcagatg
     3061 attgtttatt tgaattccca ctacatcctg ctaaaacaag taggaaagca agcgtaacaa
     3121 caagcatttt aattacttta ttcattgact aattagcctc cttcgtgatg tatgacaatg
     3181 agaatcatta tcacgattta gtatgaatta aattttttcc taagtcaata aaatatttat
     3241 gatttacatg caacttataa ttatttgaca tataaatgca taaaaaatat aatcctaatt
     3301 acttgatagt gagaatcatt atcaattagg taacacacaa tattatagaa ttttaaattt
     3361 gaggaggaag cgcttttgat tgaaaaaagt caagcatgtc acgattcatt gttagattct
     3421 gtagggcaaa cacctatggt tcaacttcat caactatttc cgaaacatga agtgtttgca
     3481 aagttagagt atatgaatcc tggaggcagc atgaaagatc gacctgccaa gtacatcatt
     3541 gaacatggta ttaaacatgg tttaatcact gagaatacac atttaattga aagtacttct
     3601 ggtaatttag gcattgcgtt ggcaatgata gctaaaatca agggattaaa actcacgtgt
     3661 gttgttgatc ctaaaatatc accaacaaat ttgaaaatta ttaaaagtta tggtgccaat
     3721 gtagaaatgg ttgaagaacc tgatgcacat gggggttatt taatgactcg tattgcaaag
     3781 gtgcaagaac tgttagccac tattgacgat gcatattgga ttaatcaata tgcgaatgag
     3841 ttaaattggc aatcccatta tcatggtgca ggcacagaga ttgttgaaac aattaagcaa
     3901 cctatagatt attttgtcgc gccagtcagc acgacaggta gcattatggg tatgagtaga
     3961 aaaataaaag aagtgcatcc aaacgcacaa attgttgctg ttgatgcgaa agggtcagtc
     4021 atttttggtg acaaacctat taatagagaa ttacctggta tcggtgctag tcgtgtaccc
     4081 gaaatattga atagatcaga aattaatcaa gtgatccatg tagatgatta tcaatctgct
     4141 ttgggctgtc gaaaactgat tgattatgaa ggcatatttg ccggaggttc aacaggctcg
     4201 attattgcag cgattgagca gttgataacg tcaattgaag aaggtgcaac aattgtcacg
     4261 attttaccag atcgaggcga tcgttactta gatttagttt attcagatac atggttagaa
     4321 aaaatgaaat caagacaagg agttaaatca gaatgaatag agagatgttg tatttaaata
     4381 gatcagatat tgaacaagcg ggaggtaatc attcacaagt ttatgtggac gcattaacag
     4441 aagcattaac agcccatgcg cacaatgatt ttgtacaacc gcttaagccg tatttaagac
     4501 aggatcctga aaatggacac atcgcagatc gaattattgc aatgccaagt catatcggtg
     4561 gtgaacacgc aatttcaggt attaagtgga taggtagtaa gcacgacaat ccatcgaaac
     4621 gtaatatgga gcgtgcaagt ggtgtcatta ttttgaatga tccagaaacg aattatccaa
     4681 ttgcagttat ggaagcaagt ttaattagta gtatgcgtac tgcagcagtt tcagtgattg
     4741 cagctaagca tttggctaaa aaaggattta aagacttaac aatcattggt tgcgggctaa
     4801 tcggagacaa gcaattacaa agtatgttag agcaattcga tcatattaaa cgcgtgtttg
     4861 tttacgatca attctctgaa gcatgtgcac gctttgttga tagatggcaa caacagcgtc
     4921 cggaaattaa ttttattgcg acagaaaatg ctaaagaagc agtatcaaat ggtgaagtag
     4981 tcattacatg taccgtaacg gatcaaccat acattgaata tgattggtta caaaagggtg
     5041 catttattag caacatttct atcatggatg tgcataaaga agtctttatt aaagctgaca
     5101 aagtcgtagt agatgactgg tcacaatgta atcgagaaaa gaaaactatt aaccaattgg
     5161 tgttagaagg taaattcagc aaagaagcac ttcatgctga actagggcaa cttgtgacag
     5221 gtgacatacc aggacgtgaa gacgatgatg aaatcatatt acttaatccg atgggtatgg
     5281 ctatcgaaga tatttcaagt gcttatttta tttatcaaca ggcacaacaa caaaatattg
     5341 ggacaacatt gaacctatat taagaatgcg aggtgtctga acattgcaga atcatacagc
     5401 agtcaataca gcacaagcga taatattaag agatttagtt gatgcattat tatttgaaga
     5461 tatagccgga attgtatcga atagtgagat tactaaagaa aacggacaaa cgcttttgat
     5521 atacgaacgt gaaacacaac aaataaagat acctgtttat tttagtgctt taaatatgtt
     5581 tcgttacgaa agttcacaac caattacgat agagggaagg gtgtctaagc aacctttaac
     5641 ggcagctgaa ttttggcaaa caattgctaa tatgaattgt gatctaagtc atgaatggga
     5701 agtggctcgc gttgaagaag gactgactac tgctgccaca cagcttgcta aacaattatc
     5761 agaattagat ttagcgtcac atccttttgt gatgtcagag cagtttgcaa gtttaaaaga
     5821 tcgtccattt catccattag ctaaagaaaa aagaggatta agagaagcgg attatcaagt
     5881 gtatcaagct gaattaaatc aatcatttcc tttaatggtt gcagcagtta aaaagacaca
     5941 tatgattcat ggcgatactg caaatatcga tgaattagaa aatttgacag tacctataaa
     6001 agaacaagcg acagacatgt taaatgatca agggttatca atagatgact atgtactatt
     6061 tccggtacat ccttggcaat atcagcatat tctgccgaac gtctttgcga cagagattag
     6121 tgaaaagttg gttgtactat taccgttaaa atttggagat tatctgtcgt cttcaagtat
     6181 gcgttcatta attgatattg gcgcaccgta taaccatgtc aaagtaccat ttgcaatgca
     6241 gtcattaggc gcattaaggc taacgcctac gcgttacatg aaaaacggag aacaagcaga
     6301 acaattatta cgtcagctta tagaaaaaga tgaagcacta gctaagtatg tcatggtttg
     6361 tgatgaaaca gcttggtggt catatatggg tcaagataat gatattttca aagatcaatt
     6421 aggtcatcta actgttcagc taagaaagta tcccgaagtg ctagccaaaa atgatacgca
     6481 acagctagtg tcaatggcag cactcgcggc aaatgatcgc actttatatc aaatgatttg
     6541 tggaaaagat aatatttcta aaaatgatgt catgacgtta tttgaagata tcgcgcaagt
     6601 ctttttaaag gtaacactat catttatgca atacggcgca ttaccagagt tgcatggtca
     6661 aaatatattg ttgtcatttg aagatggacg tgtacaaaaa tgcgtgttac gtgatcatga
     6721 tactgtcaga atttataaac catggctaac agcacatcag ctttcattgc cgaagtatgt
     6781 cgtcagagaa gatacaccta atacgctaat taatgaggat ttggaaacat tctttgctta
     6841 ttttcaaaca ttagctgtat cggtaaatct atatgccatt attgatgcaa ttcaagattt
     6901 atttggtgta agtgagcatg aacttatgtc gttgttaaaa caaattttaa aaaatgaagt
     6961 ggcaactatt tcctgggtta caactgatca gctagctgtc agacacattt tatttgataa
     7021 acagacgtgg ccattcaaac aaattttatt accattgcta tatcaacgtg atagtggtgg
     7081 aggtagtatg ccttcaggtt taactaccgt accaaatcca atggtgacat atgattaatc
     7141 agtctatatg gcgcagtaac tttcgcattt tatggctcag tcagtttata gcgattgctg
     7201 gactgacagt acttgtgcca ttattgccaa tttatatggc atcactacaa aatctatcag
     7261 tcgtagaaat acagttgtgg agtggtatag cgattgctgc tccagctgta acgacgatga
     7321 tagcttcgcc gatatggggg aagctaggtg ataagatcag ccgaaaatgg atggtcttaa
     7381 gagcgttact tggtttggcg gtatgcttat ttttaatggc attgtgtacg acaccattac
     7441 agtttgtact tgtgaggtta ttgcagggac tatttggtgg tgttgttgat gcatcaagtg
     7501 cgtttgcgag tgcagaggcg ccagctgaag atcgtggaaa ggtattagga agactgcaaa
     7561 gttcagtcag cgcagggtct cttgtggggc cattaattgg cggtgttaca gcttcgatat
     7621 taggttttag tgcgttactg atgagtattg ccgttattac ttttattgtc tgtattttcg
     7681 gtgcattaaa attgattgaa acgacacata tgccaaaatc acaaacacca aatattaata
     7741 aaggtattcg ccgttcattt caatgtctat tatgcacaca acaaacatgt cgatttatta
     7801 tcgttggcgt tttagcaaac tttgctatgt atggcatgct aactgcatta tcaccacttg
     7861 cttcatcagt gaatcataca gcgatagatg accgtagtgt gattggattt ttacagtccg
     7921 cattttggac ggcttcgata ttaagcgcgc ctttatgggg acgctttaat gataaatcat
     7981 atgttaaatc agtatatata tttgccacga ttgcatgtgg ttgtagtgcg atactgcaag
     8041 gtttagcgac gaatatagag tttttaatgg ctgcaagaat acttcaagga ttaacatata
     8101 gtgcattgat tcaaagtgtc atgtttgttg tcgtgaatgc gtgtcatcaa caacttaaag
     8161 gcacatttgt tggaacgacg aacagtatgt tagttgttgg tcaaattatt ggcagtctta
     8221 gtggcgctgc cattacaagt tatactacac cagctactac gtttatcgtt atgggcgtag
     8281 tatttgcagt aagtagttta tttttaattt gttcaaccat cactaatcaa atcaacgatc
     8341 acacattaat gaaattatgg gagttgaaac aaaaaagtgc aaaataaaga attaatacaa
     8401 catgcagcgt atgcggctat cgaacgcatt ttaaatgaat attttagaga agaaaattta
     8461 tatcaagtac cacctcaaaa tcatcaatgg tctatacaat tatcagagct cgaaacttta
     8521 acgggtgaat ttcgctattg gtctgcgatg gggcatcata tgtatcatcc agaggtatgg
     8581 cttatcgatg gaaaaagtaa aaaaataaca acttataaag aagcaattgc gcgtattttg
     8641 caacatatgg ctcaaagtgc agataatcaa acggcagtgc aacaacatat ggcgcaaatt
     8701 atgtctgaca tcgataatag cattcatcgc acggcgcgtt atttgcaaag taacacaata
     8761 gactacgtag aggatcgtta tatcgtttca gaacaatctt tatacttagg tcatccattt
     8821 catccgactc ctaagagtgc aagtgggttt tcagaagcag atttagagaa atatgcaccc
     8881 gaatgtcata catcattcca attgcattat ttagctgtgc atcaagatgt tctgctcacg
     8941 cgctatgtag aaggtaaaga agatcaggtt gagaaagtgt tgtatcaatt agcagacata
     9001 gatatatcag agatacccaa agattttatt ttattaccaa cacatcctta tcaaatcaat
     9061 gtgttgcgac agcatccaca gtatatgcaa tatagtgaac aaggtttaat aaaagacctt
     9121 ggcgtttccg gtgatttagt gtacccgacg tcttcggtta gaactgtatt ttcaaaagca
     9181 ttaaacattt atttaaaatt accgatacac gttaaaatta ctaattttat acgtacgaat
     9241 gatcttgaac aaattgaacg gacaattgat gccgcgcaag ttatcgcatc agtcaaagat
     9301 gaggttgaaa caccccattt taaattgatg tttgaagaag gatatcgtgc attgttaccg
     9361 aatccattag ggcaaacagt tgaacctgaa atggatttat taacaaatag tgccatgatt
     9421 gttcgtgaag ggataccgaa ttaccatgct gataaagata ttcatgtatt ggcgtcatta
     9481 tttgaaacga tgcctgattc accgatctct aagttagcac aagtgattga gcaaagtggt
     9541 ttagcaccag aagcatggct tgaatgttat ttggatcgta cattattgcc gatattaaag
     9601 ttgtttagta acacaggcat tagtctagaa gcacatgtac aaaatacatt aattgaatta
     9661 aaagatggca tacccgacgt atgctttgtc agagatcttg aaggcatttg tctatctaga
     9721 acgattgcta ctgaaaaaca gcttgtgcca aatgttgtgg cagcatcaag ccctgttgta
     9781 tatgcacatg atgaagcatg gcatcgtctt aaatattatg ttgtagtaaa tcacttagga
     9841 catttagtat caactattgg taaagcaact agaaatgaag ttgtgttatg gcaacttgta
     9901 gcgcatcgtc ttatgacttg gaaaaaagaa tacgcgaata acgcagtatt tgttgactgt
     9961 gtagaagatt tatatcaaac gccgaccatt gcggctaaag cgaatttgat gagtaaattg
    10021 aatgattgtg gtgcaaaccc tatttataca catataccaa atccaatttg ttataacaag
    10081 gaggtatcgt attgtgaatc aaacaattct taatcgtgta aagactagag taatgcatca
    10141 actggtatca tcacttattt atgagaatat tgttgtgtat aaagcgtcat atcaagacgg
    10201 tgtcggtcat tttacaatag aaggacatga ttcagagtat cgttttactg ctgaaaagac
    10261 acatagcttt gatcgtatac gtatcacatc accaattgag cgtgtcgtag gagatgaggc
    10321 agatacaaca acagactata cacaattatt gagagaggct gtatttacat ttcctaaaaa
    10381 tgatgaaaag ctagaacaat ttattgttga gttattacag acagaattaa aagatacaca
    10441 aagtatgcag tatcgagaat caaacccacc agcaacacct gagacattta acgactatga
    10501 attttatgcg atggaagggc atcagtatca tccaagttac aaatcacgtt taggatttac
    10561 gttgagtgat aatttgaaat ttggtcctga ttttgtacca aacgttaaac tgcagtggtt
    10621 agctatcgac aaagataaag tagaaacgac ggtatcaaga aatgttgtag ttaacgaaat
    10681 gttacgtcaa caagttggcg ataagactta tgaacatttt gtacagcaaa ttgaagcgtc
    10741 tggcaaacat gtaaatgatg ttgagatgat acctgtacac ccatggcagt ttgaacatgt
    10801 catccaagtt gatttggctg aagaaaggct taatggcaca gtactatggt taggggaaag
    10861 tgatgagcta tatcaccctc aacaatcgat tcgtacgatg tcgccaatag acacgacaaa
    10921 atattattta aaggtaccaa taagtataac gaacacttca acgaaacgag tgttggcgcc
    10981 tcatacaatt gaaaatgcag cgcaaattac ggattggtta aagcagatac agcaacaaga
    11041 tatgtattta aaagatgaat taaagacagt ttttctaggg gaagtcttag gacagtctta
    11101 tttaaataca caactttcgc cttataaaca aactcaagtt tatggtgcgt taggtgttat
    11161 atggcgtgaa aatatatatc atatgttaat cgatgaagag gatgcgatac catttaatgc
    11221 actttatgca agtgataagg atggtttacc attcattgaa aagtggatta aacaatatgg
    11281 ttctgaagct tggacaaagc aatttttagc tgtagcgatt cgtccaatga ttcatatgct
    11341 ttattatcac ggtattgcct ttgaatcgca tgcacaaaat atgatgctca ttcatgaaaa
    11401 tggttggcct acacgtattg ccttaaaaga tttccacgat ggtgttcgtt ttaagcgtga
    11461 gcatttaagt gaagcagctt cacacctgac attaaagcca atgccagaag cacataaaaa
    11521 agtgaatagt aattcattta ttgaaacaga tgacgaacgt ttagtacgcg actttttaca
    11581 tgatgcattt ttctttatta atatcgccga aatcatctta tttattgaaa agcaatatgg
    11641 tatcgatgag cagcgacaat ggcaatgggt taaagacatt atcgaggcgt atcaagaagc
    11701 atttccagag ttgaataact atcaacattt cgatttgttt gaacctacga ttcaagttga
    11761 aaagttaacg acacgtcgat tattaagtga ctccgagtta agaattcatc atgttacaaa
    11821 tccattaggt gtaggaggta tcaatgatgc aacaactatc tctgaaacat agattaaaca
    11881 atggtgattc agtttatggc atttttaatt ctataccgga cccattgatg atcgaggtta
    11941 tcgcagcaag cgggtatgac tttgttgtga ttgatacaga acacgtggcg attaatgatg
    12001 agacactagc gcatttaatt cgtgcagctg aagcagcgca tattatacca attgtacgtg
    12061 tcactgcagt gatagataga gatatcatta aagtgttaga tatgggtgcg agaggtatta
    12121 ttgtgccaca cgttaaagat cgtgagacag ttgagcatat tgtgaaatta agtcgttatt
    12181 acccgcaagg attaagaagt ttgaatggtg gtcgcatggc aagatttgga cgtacaccat
    12241 tacttgatgc aatggagatg gctaatgagc atattatggt gattgccatg atagaagatg
    12301 ttgaaggggt tatggccatt gacgatatag ctcaagtcga aggtttagac atgatagtcg
    12361 aaggtgccgc agatttatcg cagtcacttg gcataccatg gcaaacgcgt gatgatcaag
    12421 taacatcaca tgttcaacat atttttgagg ttgtgaatgc acatggtaaa catttttgtg
    12481 cattaccacg tgaagatgaa gatattgcaa aatggcaggc acaaggtgta caaacattta
    12541 ttttaggtga tgatcgcgga aaaatatatc gccatttaag tgcatctcta gcgacgtcta
    12601 aacagaaagg ggatgatggc taatgcgtat agttcaacct gttattgaac aattaaaagc
    12661 acaatctcat ccagtttgtc attatatcta tgatttagtc ggactggaac atcatttgca
    12721 acatattaca tcgtcattgc cgagtaattg tcaaatgtac tatgcaatga aagcaaatag
    12781 tgaacgaaca atcctagata caattagtca gtatgttgaa ggattcgaag ttgcatctca
    12841 aggtgaaata gcaaaaggtc ttgcttttaa accagcaaat catattattt ttggtggccc
    12901 tggtaagaca gacgaggaac taagatatgc agtaagtgaa ggtgttcagc gtattcatgt
    12961 tgaaagtatg catgaattac aacggctaaa tgccatctta gaagatgaag ataagacaca
    13021 acacatttta ttgcgtgtta atttagcagg accatttccc aatgcaacgt tgcatatggc
    13081 aggacgccca acacaatttg gtatttctga agacgaagtt gatgatgtca ttgaagctgc
    13141 gctagtaatg ccaaatattc atctagatgg ctttcatttt cattctattt ctaacaattt
    13201 agactcgaat ttacatgtcg atgtagtgaa actttatttt aaaaaagcaa aatcatggtc
    13261 tgaaaaacat cgatttccac tcaaacatat caatcttggt ggtggcatag gcgttaacta
    13321 tgcagattta actagccaat ttgagtggga taattttgta gaaaatttta aaacacttat
    13381 cgttgagcaa gaaatggaag atgtgacatt gaactttgaa tgtgggcgct ttattgtggc
    13441 acatattggt tactatgtga cagaagtgct agatattaag aaagtgcatg gtgcttggta
    13501 tgccatttta agaggaggta cgcaacaatt tagactgccg gtatcttggc agcataacca
    13561 tccttttgaa atttatcgtt ataaggacaa tccatattca tttgaaaaag tttcaatttc
    13621 gagacaggac acaacgttag tcggtcaatt atgtacaccg aaagatgtct ttgctagaga
    13681 agtacagata gacgcaatca gtacaggcga cgttattgtt ttcaaatatg caggtgcata
    13741 cggatggtct atttcacatc acgatttctt aagccatcca catcctgaat ttatttattt
    13801 aacgcaaaca aaggaggatg aataactatt gaatcatatt catgaacatt taaaattggt
    13861 accagtagat aagattgatc ttcacgaaac attcgaacct ttaagattgg aaaaaacgaa
    13921 aagtagtatt gaagcagatg attttatacg tcatcctatt ttagtgacag cgatgcaaca
    13981 tggtagatat atggttatag atggtgtgca tcggtataca agtttgaaag cgttaggatg
    14041 taagaaagtt ccagtgcaag aaatccatga aacacaatat tcaattagta catggcaaca
    14101 taaagttcca tttggtgtgt ggtgggaaac gttacaacaa gaacatcgct tgccatggac
    14161 tactgagaca agacaagaag cgccatttat tacgatgtgt catggtgata cagaacaata
    14221 tttgtatacg aaagatttag gcgaagcaca ttttcaagta tgggaaaagg ttgtcgcaag
    14281 ttatagtggt tgttgttctg tagagagaat tgcacaaggt acatatcctt gtctttctca
    14341 acaagatgta ctcatgaagt atcagccatt gagttataag gaaattgaag cggttgttca
    14401 taaaggggaa actgtgccag caggtgtgac acgctttaat atttcaggac gatgtcttaa
    14461 tcttcaagta ccactggcat tacttaaaca agatgatgat gttgaacaac tgcgtaattg
    14521 gaagcagttt ttagcagata agtttgccaa tatgagatgc tatactgaaa aagtatactt
    14581 ggtggagcaa tagttttact gtgatgttga gggaaatatg atgatttagc gtattgatag
    14641 cgaaaatata ataaaacaat atagtgtgga gaacttttga tattttataa atattgaagt
    14701 tctccatttt tgtattttgc atataaaaat taaataaaat aaggtatatt aaggtaaagt
    14761 ataaatatta aataaatggg gaatagacat ggaaaaaaat gtagaaaaat cattcataaa
    14821 g
//
LOCUS       DAHHSC010000049        14660 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.049, whole genome shotgun sequence.
ACCESSION   DAHHSC010000049 DAHHSC010000000
VERSION     DAHHSC010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 14660)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14660)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14660
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.049"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            159..362
                     /locus_tag="NBI57_001128"
     CDS             159..362
                     /locus_tag="NBI57_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000875476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1822088.1"
                     /translation="MKYKHFAIITLITGIVMLTAMYFVPFGYHKIPIFAGMAIIFTVY
                     FSNKKSIDETNIFSKKNRNKNKQ"
     gene            complement(449..610)
                     /locus_tag="NBI57_001129"
     CDS             complement(449..610)
                     /locus_tag="NBI57_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1822089.1"
                     /translation="MLLILELRFWNLEFGFRNSTFGIQNYNEWKILIIACLMTWFCSD
                     FYLMNCFFV"
     gene            complement(647..1387)
                     /locus_tag="NBI57_001130"
     CDS             complement(647..1387)
                     /locus_tag="NBI57_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HCD1822090.1"
                     /translation="MFILRNDLETLKHIIDSSNRITFFTGAGVSVASGVPDFRSMGGL
                     FDEISKDGLSPEYLLSRDYLEDDPEGFINFCHKRLLFVDTKPNIVHDWIAKLERNQQS
                     LGVITQNIDGLHSDAGSQHVDELHGTLNRFYCNACHKSYTKSDVIDRTLKHCDNCGGA
                     IRPDIVLYGEMLDQPTIIRALNKIEHADTLVVLGSSLVVQPAAGLISHFKGDNLIIIN
                     KDRTPYDSDATLVIHDDMVSVVKSLMTE"
     gene            1593..2384
                     /locus_tag="NBI57_001131"
     CDS             1593..2384
                     /locus_tag="NBI57_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCD1822091.1"
                     /translation="MTHRLNKGGNFTLNKHERLDEIAKLVNKKGTIRTNEIVEGLNVS
                     DMTVRRDLIELENKGILTKIHGGARSNSTFQYKEISHKEKHTRQIAEKRYIARKAASL
                     IEDGDTLFFGPGTTVELLAEEVNHHTLTIITNCLPVYKILLEKQTAHFRVYLIGGEMR
                     HITEAFVGEMANAMLEKLRFSKMFFSSNAVNKGAVMTSTLDEAYTQQLALSNSIEKYL
                     LIDHTKVGKEDFTSFCQLNELTAVVMDYEDEEKVETIKTYIEVVD"
     gene            2751..3179
                     /gene="lacA"
                     /locus_tag="NBI57_001132"
     CDS             2751..3179
                     /gene="lacA"
                     /locus_tag="NBI57_001132"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacA"
                     /protein_id="HCD1822092.1"
                     /translation="MAIIIGSDEAGKRLKEVIKSYLLDNKYDVVDVTEGQEVDFVDAT
                     LAVAKDVQSQEGNLGIVIDAFGAGSFMVATKIKGMIAAEVSDERSGYMTRGHNNSRMI
                     TMGSEIVGDTLAKNVVKGFVEGKYDGGRHQIRVDMLNKMC"
     gene            3195..3710
                     /gene="lacB"
                     /locus_tag="NBI57_001133"
     CDS             3195..3710
                     /gene="lacB"
                     /locus_tag="NBI57_001133"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacB"
                     /protein_id="HCD1822093.1"
                     /translation="MKIALGCDHIVTDTKMRVSEFLKSKGHEVIDVGTYDFTRTHYPI
                     FGKKVGEQVVSGNADLGVCICGTGVGINNAVNKVPGVRSALVRDMTSALYAKEELNAN
                     VIGFGGRIIGELLMCDIIDAFINAEYKATEENKKLIAKIKHLETSNADQADPHFFDEF
                     LEKWDRGEYHD"
     gene            3723..4655
                     /gene="lacC"
                     /locus_tag="NBI57_001134"
     CDS             3723..4655
                     /gene="lacC"
                     /locus_tag="NBI57_001134"
                     /EC_number="2.7.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-6-phosphate kinase"
                     /protein_id="HCD1822094.1"
                     /translation="MILTLTLNPSVDISYPLTALKLDDVNRVQEVSKTAGGKGLNVTR
                     VLAQVGEPVLASGFIGGELGQFIAKKLDHADIKHAFYNIKGETRNCIAILHEGQQTEI
                     LEQGPEIDNQEAAGFIKHFEQLLEKVEAVAISGSLPKGLNQDYYAQIIERCQNKGVPV
                     ILDCSGATLQTVLENPYKPTVIKPNISELYQLLNQPLDESLESLKQAVSQPLFEGIEW
                     IIVSLGAQGAFAKHNHTFYRVNIPTINVLNPVGSGDSTVAGITSAILNHENDHDLLKK
                     ANTLGMLNAQEAQTGYVNLNNYDELFNQIEVLEV"
     gene            4659..5639
                     /gene="lacD"
                     /locus_tag="NBI57_001135"
     CDS             4659..5639
                     /gene="lacD"
                     /locus_tag="NBI57_001135"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HCD1822095.1"
                     /translation="MSKSNQKIASIEQLSNNEGIISALAFDQRGALKRMMAKHQTEEP
                     TVAQIEQLKVLVAEELTQYASSILLDPEYGLPASDARNKDCGLLLAYEKTGYDVNAKG
                     RLPDCLVEWSAKRLKEQGANAVKFLLYYDVDDAEEINIQKKAYIERIGSECVAEDIPF
                     FLEVLTYDDNIPDNGSVEFAKVKPRKVNEAMKLFSEPRFNVDVLKVEVPVNMKYVEGF
                     AEGEVVYTKEEAAQHFKDQDAATHLPYIYLSAGVSAELFQETLKFAHEAGAKFNGVLC
                     GRATWSGAVQVYIEQGEDAAREWLRTTGFKNIDDLNKVLKDTATSWKQRK"
     gene            5661..5972
                     /locus_tag="NBI57_001136"
     CDS             5661..5972
                     /locus_tag="NBI57_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HCD1822096.1"
                     /translation="MNREEVQLLGFEIVAFAGDARSKFLEALTAAQAGDFAKADALIE
                     EGNNCIAEAHRAQTSLLAKEAQGDDIAYSVTMMHGQDHLMTTILLKDLMKHLLEFYKR
                     G"
     gene            5978..7690
                     /locus_tag="NBI57_001137"
     CDS             5978..7690
                     /locus_tag="NBI57_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HCD1822097.1"
                     /translation="MMQKLIAQIEKGKPFFEKLSRNIYLRAIRDGFISAMPVILFSSI
                     FLLIAYVPNIFGFKWDKGMEAILMKPYNYTMGLVAFLVAGTTAKSLTDSFNRKLESTN
                     QINFISTMLAAMCGFLFLASDPAKDGGFLSAFMGTKGLLTAFLSAFVTVIVYNFCVKR
                     NITIKMPKEVPPNISQVFKDLIPFSAVIIILYALDLVIRNSFKSNVAEGILKLFEPLF
                     TAADGWIGVTIIFGAFALFWFVGIHGPSIVEPAIAAITYANIEANFKLLQAGEHADKI
                     ITSGTQMFIVTFGGTGATLVVPFMFMWMTKSKRNKAIGRASVVPTFFGVNEPILFGAP
                     LVLNPVFFIPFVLAPIVNVWIFKLFVEVLGMNSFSVNLPWTTPGPLGIIMGTGFGLWS
                     FVLAITLIVVDIIIYYPFLKVYDSEILDEEEGRKESNSDLKEKVAANFDTKKADSILA
                     ASGVSDDAAKASNITEQTNVLVLCAGGGTSGLLANALNKAAEEYHVPVKAAAGGYGAH
                     MDIMKEYQLIILAPQVASNYEDIKQDTDRLGIKLAKTQGAEYIKLTRDGQAALDFVQQ
                     QFEN"
     gene            7708..9120
                     /gene="lacG"
                     /locus_tag="NBI57_001138"
     CDS             7708..9120
                     /gene="lacG"
                     /locus_tag="NBI57_001138"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HCD1822098.1"
                     /translation="MTKTLPEDFIFGGATAAYQAEGATNTDGKGRVAWDTYLEENYWY
                     TAEPASDFYNRYPVDLELSEKFGVNGIRISIAWSRIFPNGYGEVNPKGVEYYHKLFAE
                     CHKRHVEPFVTLHHFDTPEVLHKDGDFLNRKTIDYFVDYAEYCFKEFPEVKYWTTFNE
                     IGPIGDGQYLVGKFPPGIKYDFEKVFQSHHNMMVAHARAVKLFKDGGYQGEIGVVHAL
                     PTKYPFDPSNPEDVRAAELEDIIHNKFILDATYLGKYSRETMEGVQHILSVNGGKLNI
                     TDEDYAILDAAKDLNDFLGINYYMSDWMRGYDGESEITHNATGDKGGSKYQLKGVGQR
                     EFDVDVPRTDWDWMIYPQGLYDQIMRVVKDYPNYHKIYITENGLGYKDEFIESEKTVH
                     DDARIDYVRQHLNVIVDAIKDGANVKGYFIWSLMDVFSWSNGYEKRYGLFYVDFETQE
                     RYPKKSAYWYKELAETKEIK"
     gene            9386..10255
                     /locus_tag="NBI57_001139"
     CDS             9386..10255
                     /locus_tag="NBI57_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000719336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCD1822099.1"
                     /translation="MKKIFVIITTLLAVAIIIGSIITVVFSQRQAQTFKIQQQQFVKK
                     PIPTLFLHGFGGSANSEKFMVKQAEKRGVTKDIITAYVSKDGAVTFKGKLRKDAVNPI
                     VKIELENNRQGYLDKNAAWFKNVLTKLQSEYNFDKFNFVGHSMGNLTFAQYMMTYGND
                     KSLPQLNKQVNIAGTFNGVLNMNEDVNEITVDKDGKPSRMNQPYQQLRVLKDIYKGKG
                     IEVLNIYGDLKDGTHSDGRVSNSSSKSLKYLLGNSPKSYRESKYEGEPAQHSQLHENE
                     NVANELIDFLWKK"
     gene            10533..11534
                     /locus_tag="NBI57_001140"
     CDS             10533..11534
                     /locus_tag="NBI57_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HCD1822100.1"
                     /translation="MQNKQILFNKIPEGMPQEDTFKIEEIDTPKLESDGVLVQTLYIS
                     VDPYMRGRMTKADSYVQPFEIGKPIVSHVVAKVIDSTLADYKKGDVVVGMLPWRIINH
                     VQADQITKVPTTDVPLDLYLSVLGMPGQTAYHGLLDIGQPKAGDTVVVSAASGAVGSV
                     VGQIAKIKGCRVVGIAGGDKKVNYLTETLGFDAGIDYKKEDFAEALAQAVPDGIDVYF
                     ENVGGTVGDEVFKHLNRFARVPVCGAISSYNHPEADIGPRIQGTLIKKQVMMRGFLVS
                     EFANAFKEASEQLATWVQEGKIQSQVTIEDGFENAPHAFKNLFTGDNFGKQVIKVTE"
     gene            11769..12776
                     /locus_tag="NBI57_001141"
     CDS             11769..12776
                     /locus_tag="NBI57_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HCD1822101.1"
                     /translation="MKMIGFEKPFKLEEGNLFKVYEQRKPTPENDDILVKVNSISVNP
                     VDTKQRQMKVTQAPRVLGFDAIGTVEAIGPDVTLFSPGDVVFYAGSPNRQGSNATYQL
                     VSEAIVAKAPHNISANEAVSLPLTGITAYETFFDTFKISHNPSENVGKSVLIINGAGG
                     VGSIATQIAKRYGLTVITTASRQETTEWCEKMGADIVLNHKEDLVRQFKEKEIPLVDY
                     IFCTYNTDLYYNTMIELIKPLGHITTIVAFNEDQDLNALKLKSITFTHEFMFARPIHR
                     TPDMIKQHEYLEDITKNIELGHYQPTTTQVFEGLSPENLYQAHQLLEKQSMIGKLVIN
                     I"
     gene            13084..14646
                     /locus_tag="NBI57_001142"
     CDS             13084..14646
                     /locus_tag="NBI57_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BCCT family transporter"
                     /protein_id="HCD1822102.1"
                     /translation="MKNKSVSLVFWVSLVICTIFVAFGAIFPKQLEKLTQNITTFIAL
                     HFSWYYLLLVLVILFVCVYILFSRYANITLGEEGEDPEFSLPSWFAMLFSAGMGIGLV
                     FWTTAEPISHAFKLTPIHKAGTQSAINDAMQFSFFHWGIHAWAVYGIVALVFAYFSFH
                     KGYPGLVSATLTPLLGEKAMRGPLGGAIDVLAVIATVTGVAATLGFGALQINEGLHFL
                     FNVPSNFTMQVILIVIATILFTWSAWSGIDKGIKTLSNINMLLAFVVLIGLFIVGPTL
                     YILNTFTNGLGNYIANFFSMSLRIPSGGQKFQWLQNWTIFYWAWWISWAPFVGIFIAR
                     VSKGRTIKEFILGVLFVPALVCFIFFAVFGASAIYLQDNHIADIAKAATETATFATLQ
                     HYPLGFVLSLITLLVIMIFFVTSADSATYVLGMLSSRGDINPKSFVKVSWGIIMALFA
                     IIMIYTGGTQAIQNLLIIAALPFSVVIIAMIWSLLKSLSEEKPRNSNKVLIKHRDPDI
                     LEYRSSKHIDEN"
BASE COUNT         5145 a         2153 c         2808 g         4554 t
ORIGIN      
        1 attttattac tcttaaaaag tgaatggagc aagcaaataa gttattatga atttttgtat
       61 tatgcaataa cttacctaag ataataatta tagtattgat aattttataa aatgaagtat
      121 ataatgaatg tagaataaat tgtatagggg ataataacat gaaatataaa cattttgcca
      181 ttattacttt aattacaggc atcgtcatgc tcacggcaat gtactttgta ccatttggat
      241 atcataaaat accaatattt gcaggaatgg cgattatttt tacagtctat tttagtaata
      301 aaaaatctat tgatgaaaca aatatcttta gtaagaaaaa tcgcaataaa aataaacaat
      361 aaaatgagtt gtaagcataa gatttacagt taatttttta cgaatgggcc tttagcgatt
      421 gaaaatgtta taaagtagca tcccaacatt aaacaaaaaa acaattcatc aaataaaaat
      481 cgctacaaaa ccaagtcatt aaacacgcaa taattaaaat tttccactca ttataattct
      541 gaattccaaa tgtcgaattc cgaaaaccaa actccaaatt ccaaaaacgc aactccaaaa
      601 ttaaaagcat ctccctgtta ctcgggaaat gctttttacc tactgattac tctgtcatta
      661 atgattttac aacggaaacc atgtcgtcat gtatgaccaa agtagcgtcg ctatcataag
      721 gtgttcgatc tttattgata ataattaaat tgtcgccttt aaaatgtgat attaatcctg
      781 cggcaggttg tacaacgagt gatgaaccta gtacaacaag ggtgtcagca tgttcaattt
      841 tatttaatgc ccttatgatg gtaggttgat ctaacatttc accgtataat acgatgtccg
      901 gtcgaatggc accaccacaa ttatcacaat gtttcaaagt tctatcaata acatctgact
      961 tcgtataaga tttatgacat gcattacaat aaaaacgatt taacgtgcca tgtaattcat
     1021 caacatgttg acttccagcg tctgagtgca aaccatcgat attttgcgtg atgacaccta
     1081 aagattgttg attacgttct aattttgcaa tccaatcatg aacgatatta ggcttcgtat
     1141 cgacaaatag taaacgctta tggcagaaat tgataaaacc ttcaggatca tcttctaaat
     1201 aatcacggct taacaagtat tctggcgaaa gtccatcttt tgaaatttca tcaaataagc
     1261 cacccattga acggaaatct ggaacgccac ttgcgacaga tacaccagca cctgtgaaaa
     1321 atgtaatacg attcgaacta tctataatat gttttaatgt ctctaaatca tttctcaaaa
     1381 taaacacccc atcaaaataa tgaatgattt ataacgtctt ttgcttttat tatagtatat
     1441 tcattttacg attatcaaaa gcgcagttta aacagttctt tttaaaaatg aattatacat
     1501 ataatcgaac agttaaatta tattagtttt tacgcaattt ttattataga ataagcatgt
     1561 atattaaaat tgatgtctaa acataaagga taatgaccca tcgcctaaat aaaggaggca
     1621 attttacatt gaataaacat gaacgtttgg acgaaattgc taaactagtg aataaaaagg
     1681 gcacgataag aacgaatgaa atcgtcgaag gtttaaatgt gtctgatatg acagttcgaa
     1741 gagatttaat tgagttggaa aataagggca ttttaacgaa gattcatggt ggtgcacgca
     1801 gtaattcaac atttcagtat aaagaaatat cacataaaga aaaacatact cgtcaaattg
     1861 ctgaaaaaag atatatagct aggaaagctg catcattaat tgaagatggg gatactttgt
     1921 ttttcggacc gggaacaaca gtggaactat tagcagaaga agtcaatcat catacgctca
     1981 caattattac gaattgtttg ccggtgtata aaattttgtt agaaaaacaa acagcacatt
     2041 ttcgtgtcta tttaattggt ggtgaaatgc gccatattac agaagcattt gtaggtgaaa
     2101 tggcgaatgc catgttggaa aaactaagat ttagcaagat gttctttagt agtaacgcag
     2161 taaataaagg tgctgtgatg acatctacat tagatgaagc ctatacgcaa caattggcac
     2221 taagtaattc aattgaaaaa tacttgttaa tcgatcatac gaaagttggc aaagaagatt
     2281 ttacatcatt ttgtcagtta aacgaattga ctgcagtggt catggactat gaagatgaag
     2341 aaaaagtaga aacgattaaa acatatattg aagtagtcga ctaaattaaa agtataactg
     2401 cattgttaaa atatgtatct atatgctcat aagtgaatgt gaatttgcat tcatttatgg
     2461 gttatttttt tgcaaattta tgtaaacggg aacatcgaca aagttttact acaatttcaa
     2521 acgaatacct aattaaaaca acaccacaat aggaatgttc acttattgtt tgaaaatatg
     2581 tcataaacaa acagattata aacatattat gttgatttat tgtttgttta tgtttataat
     2641 ttaaacgtaa tcaaattgaa agccttttct caagattttc ataagctaca aataaatatg
     2701 tcatatttta agaaaggtat ttcaaaatta aagtaaaaag gagtcttatt atggcgatta
     2761 ttattggttc agatgaagct ggcaaacgat taaaagaagt catcaaatca tacttattag
     2821 acaataaata tgatgttgtt gacgtaacag aaggacagga agttgacttt gttgatgcaa
     2881 ctttggctgt agcaaaagat gttcaaagtc aagaaggtaa cttaggtatt gttatcgatg
     2941 catttggtgc aggtagcttt atggttgcta caaaaatcaa aggcatgatt gctgctgaag
     3001 tatcagatga acgctctgga tatatgacta gaggccataa caattcaaga atgatcacta
     3061 tgggatctga aattgttgga gacacattag cgaaaaatgt cgttaaaggt tttgttgaag
     3121 gtaaatacga tggtggtaga caccaaatcc gcgtagatat gcttaataaa atgtgctaag
     3181 gaggaaaaat tactatgaag attgcattag gatgcgacca tattgttaca gatacaaaaa
     3241 tgcgtgtatc tgaattttta aaatcaaaag gacatgaagt cattgacgta ggaacatacg
     3301 attttacaag aacacattat ccaatttttg gtaaaaaagt tggcgaacaa gttgttagcg
     3361 gtaatgcaga cttaggtgtt tgtatttgtg gaacaggtgt tggtattaac aatgctgtaa
     3421 ataaagtacc tggcgttcgt tcagcactag tacgtgatat gacatcagcg ttatatgcta
     3481 aagaagaatt aaatgcgaac gttattggct ttggtggacg tattataggt gagttattaa
     3541 tgtgcgatat tatcgatgca tttattaacg ctgaatataa agcaactgaa gagaacaaaa
     3601 aattaatcgc taaaattaaa catttagaaa caagcaatgc agatcaagct gatccacatt
     3661 tctttgatga attcttagaa aaatgggaca gaggcgaata ccacgattaa gggacgacac
     3721 atatgatttt aactttaaca ttaaatccat ccgttgatat ttcatatcca ttaacagcat
     3781 tgaaattaga tgatgttaat cgtgttcaag aagtaagtaa aacagctggt ggtaaaggtt
     3841 taaatgtaac aagagtactt gcgcaagtag gcgagccagt ccttgcgagt ggttttattg
     3901 gcggtgaatt aggtcaattt attgctaaaa aattagatca tgccgacatc aagcatgcat
     3961 tttataacat taagggtgaa acacgaaact gcattgcaat tttacatgaa gggcaacaaa
     4021 cagaaatttt agagcaggga ccggagattg ataatcaaga ggctgcagga tttattaaac
     4081 attttgaaca attattagaa aaagttgaag cagttgctat ttcaggtagc ttacctaaag
     4141 gattaaatca agattattat gcacaaatta ttgaacgatg ccaaaacaaa ggtgtgcctg
     4201 tgatattgga ctgctcgggt gcaactttgc aaacagtttt ggaaaatcca tacaaaccaa
     4261 cagtcattaa accaaacatt tctgaactat atcaattgct taaccagcca cttgatgaaa
     4321 gtttagaaag tttgaaacag gcagtgtcac aacccttatt cgaagggatt gagtggatta
     4381 ttgtgtcttt aggtgcacaa ggtgcattcg ctaagcacaa tcatacattc tatagagtga
     4441 atattccgac aataaacgta ttaaatccgg ttggttcagg tgattctaca gttgctggaa
     4501 ttacttcagc aattctcaat catgaaaacg accacgattt actaaaaaaa gctaatactt
     4561 taggcatgct gaatgcgcaa gaagcacaaa caggatatgt caatttaaat aattatgatg
     4621 aattattcaa tcaaattgaa gttttagagg tgtaaataat gtcaaaatct aatcaaaaaa
     4681 tcgcatcaat tgaacaatta agtaataacg aaggtatcat ttctgcatta gcatttgacc
     4741 aacgtggtgc tttaaaacga atgatggcta aacatcaaac ggaagagcca actgtcgctc
     4801 aaattgaaca attaaaagta ttggttgcgg aagaattaac acaatatgcg tcttcaattt
     4861 tattagatcc agaatacggt ttaccagcat ctgatgcacg taacaaagat tgtggattat
     4921 tgctagctta tgaaaaaact ggttatgacg taaatgctaa aggtcgttta cctgactgtt
     4981 tagtagagtg gtctgcaaaa cgtttaaaag aacaaggtgc aaatgctgtt aaattcttac
     5041 tttactatga tgttgatgat gctgaagaaa ttaacattca aaagaaagca tacattgaaa
     5101 gaatcggttc agaatgtgtt gcagaagaca ttcctttctt cttagaagta ttaacatacg
     5161 atgacaacat tcctgataac ggtagtgttg aattcgcaaa agttaagcct agaaaggtta
     5221 acgaagcaat gaaattattc tcagaaccac gctttaatgt tgatgtattg aaagttgaag
     5281 taccagtaaa catgaagtat gtagaaggct ttgctgaagg tgaagtggtt tatacaaaag
     5341 aggaagcggc acaacacttt aaagatcaag atgcagcaac tcatttacca tacatttact
     5401 taagtgctgg tgtttctgcg gaattattcc aagaaacttt aaaatttgct cacgaagctg
     5461 gtgctaaatt caacggtgta ctttgtggcc gtgctacatg gtctggtgct gttcaagtat
     5521 acattgaaca aggtgaagat gctgctcgtg aatggttacg tacaactggt ttcaaaaata
     5581 ttgatgactt aaataaagta ttaaaagata ctgcgacatc ttggaaacaa agaaaataag
     5641 tataaaaggg gattaatgct atgaatagag aagaagtcca attattaggt tttgaaattg
     5701 ttgcatttgc aggggatgca cgttctaagt ttttagaagc attgacagca gctcaagctg
     5761 gagattttgc aaaagcagat gcattgattg aagaaggaaa caattgcatt gctgaagcgc
     5821 atagagcaca aacaagcctg ttagctaaag aagcgcaagg tgatgatatt gcatacagtg
     5881 taacgatgat gcatggtcaa gaccatttaa tgacaacaat tttactgaaa gatttaatga
     5941 agcatttatt agaattttat aaaagagggt gatgacaatg atgcaaaaat tgatcgcaca
     6001 aattgaaaaa gggaagccat ttttcgagaa attatcacgt aacatatatt taagagccat
     6061 ccgtgatgga tttatttctg caatgcctgt catcttattc tcaagtatct ttctattaat
     6121 tgcatacgtg ccaaatatat ttggttttaa atgggataaa ggcatggaag ccattttaat
     6181 gaaaccatat aactatacga tggggcttgt agcattttta gtagcaggaa cgacagcgaa
     6241 atctttaaca gattcattta accgtaagct agaaagtaca aatcaaatta actttatttc
     6301 tacaatgcta gcagcgatgt gtggattctt gttcttagct tctgatccag ccaaagatgg
     6361 tggattctta agtgcattta tgggaactaa aggtttatta acagcatttt tatcagcatt
     6421 tgtaactgtt attgtttata acttctgtgt gaagcgcaat attacaatta aaatgccgaa
     6481 agaagtaccg ccgaatattt cacaagtatt taaggactta attccatttt cagcggtaat
     6541 catcattctt tatgcattag atttagtcat tcgcaacagc tttaaatcaa atgtagcgga
     6601 aggtatttta aaattattcg aaccattatt tacagcagca gatggatgga ttggtgtcac
     6661 aattatcttt ggtgcctttg cattattctg gtttgtagga attcatggtc catcaatagt
     6721 agagccagca attgcagcca ttacatatgc gaatatcgaa gcgaacttca agttgcttca
     6781 agctggagaa cacgcagata aaattattac atcaggtaca caaatgttta tcgttacttt
     6841 tggaggtacg ggtgcaacat tggtcgtccc gttcatgttt atgtggatga cgaaatctaa
     6901 acgaaacaaa gcgattggac gtgcatcagt tgtaccaaca ttctttggtg taaacgaacc
     6961 aattttattt ggtgcaccac tagtattgaa tcctgtgttc tttattccat ttgtattagc
     7021 accaattgtt aacgtatgga ttttcaaact tttcgttgaa gtgttaggaa tgaatagttt
     7081 tagtgtaaat ttaccttgga caacaccagg tccattgggc attatcatgg gtacaggttt
     7141 tggtttatgg tcattcgtac tagctattac tttgattgtt gtagatatta ttatttacta
     7201 cccattccta aaagtttatg atagtgaaat tcttgatgaa gaagaaggac gtaaagaaag
     7261 taattcagat ttaaaagaaa aagttgcagc aaactttgat acgaaaaaag ctgattcaat
     7321 tttagcggca agtggtgtat cagacgatgc agctaaagct tcaaatatca cagaacaaac
     7381 aaatgtctta gtactttgtg ccggtggtgg tacaagtgga ttattagcca acgcacttaa
     7441 taaagcagct gaagaatatc atgtgccagt gaaagcagca gctggcggat acggcgcaca
     7501 tatggacatt atgaaagagt accaattaat cattttagcg ccacaagttg cgtctaacta
     7561 tgaagatatt aagcaagata cagatagatt aggtattaaa ttagcgaaga ctcaaggtgc
     7621 tgaatatatc aaattgacac gagacggcca agcagccctt gatttcgttc aacaacaatt
     7681 tgaaaattaa gaataggagt ttttcatatg actaaaacat tacctgaaga ttttattttt
     7741 ggtggagcaa cagcagcata tcaagcagaa ggtgcgacaa atacagatgg taaaggtcgt
     7801 gttgcatggg atacgtattt ggaagaaaac tattggtaca ctgcagaacc agcgagtgat
     7861 ttttataaca gatatccagt ggatttagaa ttaagtgaaa aattcggtgt taatggtatc
     7921 cgtatttcaa ttgcatggtc acgtatcttc ccaaatggat atggcgaagt taaccctaaa
     7981 ggtgttgagt attaccataa gttatttgca gagtgtcata aacgtcatgt tgaaccattt
     8041 gtaacattgc atcattttga tacaccagaa gtgttacaca aagatggcga ttttctaaat
     8101 cgtaaaacga ttgactactt tgtagattat gctgaatatt gttttaaaga attcccagaa
     8161 gtaaaatatt ggactacatt caatgaaatt ggacctattg gtgatggtca atatttagta
     8221 ggtaaattcc ctccaggtat taaatatgat tttgaaaaag ttttccaatc acatcacaat
     8281 atgatggttg ctcatgcaag agcagtaaaa ttatttaaag atggcggcta tcaaggagaa
     8341 attggtgttg tacacgcatt gccaacaaaa tatccgttcg atccatcaaa tcctgaagat
     8401 gttcgtgcag cagaattaga agatatcatc cataataaat tcatcttaga tgcaacatat
     8461 ttaggtaagt attctcgtga aacgatggaa ggtgtgcaac atatcttatc tgttaatggc
     8521 ggtaaattaa atattacaga tgaagattat gccattttag atgcagccaa agatttaaac
     8581 gacttcttag gtatcaatta ctacatgagt gactggatga gaggttacga tggagaatct
     8641 gaaatcacgc ataatgcgac aggtgataaa ggcggttcta aataccaact taaaggtgtc
     8701 ggacaacgtg aatttgatgt cgacgtacct cgcacagact gggactggat gatctatcct
     8761 caaggtttat atgatcaaat catgcgtgtt gttaaagatt atcctaacta tcacaagatt
     8821 tatatcactg aaaatggttt aggatataaa gatgaattta ttgaatctga aaaaacagtt
     8881 catgatgatg cacgtattga ttatgtaaga caacatttga acgttattgt agatgctatt
     8941 aaagatggtg caaatgttaa aggttacttt atttggtcat taatggatgt gttctcttgg
     9001 tcaaatggtt atgaaaaacg atatggttta ttctatgtcg attttgagac tcaagaacgt
     9061 tatcctaaga aaagtgctta ttggtacaaa gagttagccg aaacaaaaga aattaaataa
     9121 ttatagatat taaaaggcct atcagtcgag tgctggtagg tctttattta tagaaagaga
     9181 taaattaaac aagtatatac atatttatat attaatcaat gggtgacaca ccattgaaag
     9241 ctaaagaagc aatcaattaa caaacgttaa cggacaactt tccaaagtca tcttaaaagt
     9301 taatgatgta ttcaaaatca actaaaggac atttttaatc acatccttta tacttatata
     9361 aaagacatga atgggagtta aaagtgtgaa gaaaattttt gtaattatta cgacattgtt
     9421 agcggtcgct attattatag gctctattat tacggtggtg tttagtcaac gacaggcaca
     9481 aacatttaaa atacaacaac agcaatttgt taaaaagcca attccaacat tgttcttaca
     9541 tggatttggt ggtagtgcca attctgaaaa gtttatggtg aagcaagcag aaaaaagagg
     9601 ggtaactaaa gatatcatca ctgcttacgt ttcaaaggat ggtgcagtga catttaaagg
     9661 gaaactaaga aaagatgcag tgaatcctat tgtgaaaatt gaacttgaaa ataacagaca
     9721 aggctatttg gataagaatg ctgcgtggtt taaaaatgta ctgactaagt tacaatctga
     9781 atacaatttc gataaattca actttgtcgg tcactcaatg gggaacttaa catttgcaca
     9841 atatatgatg acatatggta acgacaagtc actgccacag ttaaacaaac aagtgaatat
     9901 tgcagggaca tttaatggtg ttttaaatat gaatgaagat gtcaatgaaa ttacagttga
     9961 taaagatggt aaaccaagtc gaatgaatca accttatcag caattacgcg tgctaaaaga
    10021 tatttataaa ggtaaaggta tcgaagtgct aaatatttat ggagatttga aagatggtac
    10081 acattcagac ggcagagtgt ctaatagttc ttcgaaatca ttaaaatatt tactgggaaa
    10141 tagtccgaaa agttatcgag aatctaaata tgaaggtgag cctgcacaac atagtcagct
    10201 tcatgagaat gaaaatgttg ctaatgagtt aatcgacttc ttatggaaaa aataaagcaa
    10261 ctgctagttt tctggggcaa aaaagcttgt aatattagag cttttcaaaa attatctatt
    10321 gtttgagagt ttccataaaa tgtctaagtc gtaacatttt taatttatgt ttacagcaaa
    10381 tataatgtac ttgaagttaa atgaatcagt gcttaagtca aaatataaca actaagaatg
    10441 tatttgcata cagacatcac gaagacacaa ttaaaactta acgcttaagt aacaaataag
    10501 taaaagtttc aaataaagaa aggtgattaa tgatgcaaaa taaacaaatt ctttttaaca
    10561 aaataccaga aggcatgcct caagaggata cattcaaaat tgaagagatt gacacgccta
    10621 aacttgagag cgatggtgta cttgtacaaa cgctgtatat ttcagtggat ccatatatga
    10681 gaggacgtat gactaaggca gattcgtatg tacagccttt tgaaattggt aaaccgattg
    10741 ttagccatgt cgttgcaaaa gtaattgatt cgacactcgc agattataaa aaaggagatg
    10801 tcgttgtagg tatgttgcct tggcgaatca ttaatcatgt ccaagcggat caaattacta
    10861 aagttccaac gacagatgta ccacttgatt tatatttaag tgttttagga atgccaggac
    10921 aaacagcata tcatggtctg cttgatattg gacaacctaa agccggtgat actgttgtgg
    10981 tttcagctgc atctggtgct gtaggttcag ttgtaggtca aattgctaag attaaaggtt
    11041 gccgtgtagt aggtattgcg ggtggcgaca aaaaagtaaa ttatttaaca gagacactgg
    11101 gctttgacgc tggtattgat tataagaaag aggattttgc agaagcgtta gcacaagcag
    11161 taccagatgg tattgatgtt tattttgaaa atgttggtgg tacagttggc gatgaagtat
    11221 ttaagcattt aaatcgtttt gcacgtgtac cagtatgcgg cgcaatttcg tcatataatc
    11281 atcctgaagc agatatcgga ccacgtattc aaggtacgct tattaagaag caagtaatga
    11341 tgagaggctt cttagtaagt gaattcgcta atgcttttaa agaagctagt gaacagctcg
    11401 caacatgggt tcaagaaggt aaaattcagt cacaagtaac cattgaagat ggttttgaaa
    11461 atgcgccaca tgcatttaaa aatttattta ctggcgataa ctttggtaaa caagttatta
    11521 aagtaacgga ataataaagt atacgtttta aaaaactaac tttgttaatt gatcaaaatt
    11581 taataacttt cttatctaaa gcaaatttgt tcagtaacat ggtagaaata agtctaaaat
    11641 gtaatacgaa agtcgtctgt aattatttat atattcaaat gaaaaaggag tcatgatgct
    11701 gcggtataag cacacatggc ttctattttg tataataaga aataagttca taaatgaggt
    11761 gaaacgatat gaaaatgata ggatttgaaa agccctttaa attggaagaa ggaaatttat
    11821 ttaaagtata cgaacaaaga aagccaacgc cagaaaacga tgatatctta gtcaaagtaa
    11881 atagtattag tgtaaatcct gtagatacaa aacaacgtca aatgaaagtc actcaagcac
    11941 ctcgtgtact tgggtttgat gctattggta ctgttgaagc tatcggacca gacgttacat
    12001 tatttagtcc gggtgacgtt gtattttatg ctggatcgcc aaatagacaa ggatcaaatg
    12061 cgacatatca attggtgtca gaagctatag ttgcaaaggc accacacaac ataagtgcta
    12121 atgaagcggt tagtctaccg ctaactggta ttacggcata tgaaacgttt tttgatacat
    12181 ttaaaatttc acataatcct tctgaaaatg taggtaaatc cgtgttaatc attaatggtg
    12241 ccggtggtgt aggcagtata gccactcaaa ttgctaaacg gtatggttta actgtcatta
    12301 caacagcatc acgacaagaa acaacagagt ggtgtgaaaa gatgggcgca gatatcgtat
    12361 tgaatcataa agaggattta gtgcgtcaat ttaaagaaaa agaaattcca ttagtagatt
    12421 atatattttg cacttataat acagacttgt attacaatac tatgattgaa ctgataaaac
    12481 cattagggca tattacgact atcgtagctt ttaatgaaga tcaagattta aatgcattaa
    12541 aattaaaaag tataacattt acacatgaat ttatgtttgc gcgtccaatt catcgaacgc
    12601 ctgatatgat taaacaacat gagtatttag aagatattac gaaaaatata gaattagggc
    12661 attaccaacc aacaacgaca caagtttttg aaggtttatc acctgaaaac ttatatcaag
    12721 cacatcaatt gttggaaaaa caatcaatga ttgggaaatt agtaattaat atatagatta
    12781 gtaagtaaag tcgccttatg cagaaaatac tgtatagggc gacttatttt ttgtgtctag
    12841 cgactattga ttctaaacgg aaatatggca actttacagg tatcaaatca ataatgcact
    12901 agaaaggcat tacttaaata caataaaata ctttgttgct caatgataaa taatttgagt
    12961 gtgttttata tatcattttt aaaagatgag gcattattaa ttgtgtctat ataaaattac
    13021 aaatgattaa aatgttgcag ttatgggtat gaacttacca ttacacagaa agggtttgta
    13081 tttatgaaaa ataaatcagt aagtctagtg ttttgggtat ctttagtgat atgtacgata
    13141 tttgttgctt ttggagcaat atttccgaaa caattagaaa aactcacgca aaacattact
    13201 acatttattg cgctacattt ttcttggtat tatttattgc tcgttttagt tattttattt
    13261 gtctgtgtgt atattttatt ttcgagatac gcaaacatta cattaggtga agaaggcgaa
    13321 gatcctgaat tctcattgcc gtcttggttt gctatgttat ttagtgctgg tatgggaata
    13381 ggacttgtgt tctggacaac ggcagaacca atcagtcatg ccttcaaatt aacgcctatt
    13441 cataaagctg gcacgcaatc tgcaattaat gatgcaatgc agttttcatt tttccattgg
    13501 gggattcatg catgggcagt ctatggtatt gtcgcgcttg tgtttgcata ttttagcttt
    13561 cataaaggat atcctggctt agtcagcgct acattgacgc ctttattagg tgaaaaggca
    13621 atgcgtggac ctttaggtgg tgccattgat gtattggcag tcatagctac agtaacaggc
    13681 gttgctgcaa cattaggttt cggtgcattg caaataaacg aaggtttaca ttttttattt
    13741 aatgtaccga gtaacttcac gatgcaagta atcttgattg tcattgcaac catattattt
    13801 acttggtcag catggtcagg tatcgataaa ggtatcaaaa cgttgagtaa tatcaacatg
    13861 ttacttgctt ttgtagtact tattggtctt tttattgttg ggccaacgct ttacatttta
    13921 aatacattta caaatggtct aggtaattat attgcaaatt tctttagtat gagtttacgt
    13981 attccgtcag gcggtcaaaa attccaatgg ttacaaaatt ggacaatctt ttactgggca
    14041 tggtggatat cttgggcacc atttgtaggt atatttattg cacgtgtgtc aaaaggacgt
    14101 acgattaaag aatttattct aggtgtactt tttgtcccgg cgttagtttg ctttatattc
    14161 tttgcggtat tcggcgcgtc agcaatttac ttacaagata atcatattgc cgatattgct
    14221 aaagcagcga cagaaacagc aacttttgca acgctacaac attacccact aggttttgta
    14281 ttaagcttaa ttactttact tgttattatg attttctttg ttacatcagc ggattcggca
    14341 acttatgttt taggtatgtt gagttcaaga ggtgatatta atccgaagtc atttgtcaaa
    14401 gtgagttggg gtatcattat ggcattattt gcaattatca tgatctacac aggtggtact
    14461 caagcgattc aaaatttatt aatcattgct gcgttaccat tttccgtggt cattatagcg
    14521 atgatatggt cgcttttaaa atctttaagt gaagaaaaac ctagaaatag taataaggtt
    14581 ttaattaaac atcgtgaccc agatatttta gaatatcgtt cttcaaaaca tattgacgaa
    14641 aattaattaa taatgaggtt
//
LOCUS       DAHHSC010000050        14297 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.050, whole genome shotgun sequence.
ACCESSION   DAHHSC010000050 DAHHSC010000000
VERSION     DAHHSC010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 14297)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14297)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14297
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.050"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            341..1132
                     /locus_tag="NBI57_001143"
     CDS             341..1132
                     /locus_tag="NBI57_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001602611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HCD1822103.1"
                     /translation="MEQFYQLGWTLDSAGGASGEAYMAEQDGQKLFLKRNSNPFIAAL
                     SAEGIVPKLVWTKRIETGEVVTAQHWKNGRELSSNEMKQTRVAHLLKKIHNSRPLLSM
                     LKRMEMEPITPEIMLNKINASLSREVLTHHIVRKSLTYLEEHIPSLDSRFFTVVHGDV
                     NHNNWLLSDRDELFLVDWEGAMIADPAIDIGMLLYNYVPQQQWSEWLETYGVQESLNL
                     NKRMKWYTVIQSIGLVQWYEEQKRYKDMNTWLKFLNEVMNSNMFI"
     gene            1147..1791
                     /gene="trmB"
                     /locus_tag="NBI57_001144"
     CDS             1147..1791
                     /gene="trmB"
                     /locus_tag="NBI57_001144"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HCD1822104.1"
                     /translation="MRVRYKPWAEDYLKDHPELVDMEGQHAGKMTEWFDKTQPIHIEI
                     GSGMGQFITTLAAQNPHINYISMEREKSIVYKVLDKVKEMGLTNLKIICNDAIELNEY
                     FKDGEVSRIYLNFSDPWPKNRHAKRRLTYHTFLALYQQILNDEGDLHFKTDNRGLFAY
                     SLESMSQFGMYFTKINLNLHQEDDGSNILTEYEKKFSDKGSRIYRMEAKFHSQK"
     gene            2262..3104
                     /locus_tag="NBI57_001145"
     CDS             2262..3104
                     /locus_tag="NBI57_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HCD1822105.1"
                     /translation="MKIGDISIHYLNGGNTKMDGGAMFGVVPKPLWSKQYNANERNQI
                     NLPTHPILIQTAQYNLIIDAGIGNGKLSEKQLRNFGVDEESHIIADLANYNLTPKDID
                     YVLMTHMHFDHAAGLTDQAGHAIFENAIHVVQQDEWHEFIAPNIRSKSTYWDKNKGDY
                     SNKLILFEKHFEPVPGIKMQHSGGHSFGHTIITIESQGDKAVHMGDIFPTTAHKNPLW
                     VTAYDDYPMQSIREKERMIPYFIQQQYWFLFYHDENYFAVKYSDDGENIDAYILRETL
                     VDNN"
     gene            complement(3229..3540)
                     /locus_tag="NBI57_001146"
     CDS             complement(3229..3540)
                     /locus_tag="NBI57_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HCD1822106.1"
                     /translation="MTKLKYIIPTIIAVAIVIISTISIIQFINRKRYNPVKVLNEVKS
                     YFMNVKGSYIVYEPFVHPETDKYRLVYQGGITTIKNGQNIHYDFYADAYTGEVINIVE
                     R"
     gene            3627..4703
                     /locus_tag="NBI57_001147"
     CDS             3627..4703
                     /locus_tag="NBI57_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HCD1822107.1"
                     /translation="MNINKKVTLQRIQTLTELHGAPGFEEEVKNYMTQQMAPYVDEFI
                     ENRMGGFFGVKKSKNPNAKRVMIAAHMDEIGFMITNITKNGMIQFTNLGGVANDIWQG
                     QRLVIKNRNGDKIIGVVSNIPKHFRTGSEGAPEIKDLTLDIGAQNEDEVRERGIDIGD
                     TIVPHTPFTQLSEHRYSAKAWDNRYGCVLAIEILELLKDIELDVDLYVGANVQEEVGL
                     RGAKASAEMIDPDVAFVVDCSPANDVKGNQPLSGELGKGTLIRIKDGTMILKPVFRDY
                     LLKLVEAHDIEHQYYMSPGGTDGGEIHKANIGIPTAVIGVCARYIHSTDSVFDIRDYF
                     AARSLLSEAICNLDNNQIETLQYK"
     gene            4768..5079
                     /locus_tag="NBI57_001148"
     CDS             4768..5079
                     /locus_tag="NBI57_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HCD1822108.1"
                     /translation="MKQLESEQQFESLKQGATVFEFTAGWCPDCRVIEPDLPELEARY
                     PMFDFVSVDRDKFMDICIENGIMGIPSFLVYKNGELLGSYIGKERKSIEQIDAFLAQY
                     V"
     gene            5179..6036
                     /locus_tag="NBI57_001149"
     CDS             5179..6036
                     /locus_tag="NBI57_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1444 domain-containing protein"
                     /protein_id="HCD1822109.1"
                     /translation="MNTFQMRDKLKERLSHLDVDFKFNREEETLRIYRTDNNKGITIK
                     LNAIVAKYEDKKEKIVDEIVYYVDEAIAQMADKTLESISSSQIMPVIRATSFDKKTKQ
                     GVPFIYDEHTAETAVYYAVDLGKSYRLIDESMLEDLKLTEQQIREMSLFNVRKLSNSY
                     TTDEVKGNIFYFINSNDGYDASRILNTAFLNEIEAQCQGEMLVAVPHQDVLIIADIRN
                     KTGYDVMAHLTMEFFTKGLVPITSLSFGYKQGHLEPIFILGKNNKQKRDPNVIQRLEA
                     NRRKFNKDK"
     gene            6065..6661
                     /locus_tag="NBI57_001150"
     CDS             6065..6661
                     /locus_tag="NBI57_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4479 domain-containing protein"
                     /protein_id="HCD1822110.1"
                     /translation="MNLFYNPKYVGDVAFLQIEPVEGELNYNKKGNVVEITNEGNVVG
                     YNIFEISKDITIEEKGHIKLTDELVNVFQKRISEAGFDYKLNADLSPKFVVGYVETKD
                     KHPDADKLSVLNVNVGNDTLQIVCGAPNVEAGQKVVVAKVGAVMPSGMVIKDAELRGV
                     ASSGMICSMKELNLPNAPEEKGIMVLNDSYEIGQAFFE"
     gene            6682..10506
                     /locus_tag="NBI57_001151"
     CDS             6682..10506
                     /locus_tag="NBI57_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HCD1822111.1"
                     /translation="MSWFDKLFGEDNDSNDDLIHRKKKRRQESQNIDNDHDSLLPQNN
                     DIYSRPRGKFRFPMSVAYENENVEQSADTISDEKEQYHRDYRKQSHDSRSQKRHRRRR
                     NQTTEEQNYSEQRGNSKISQQSIKYKDHSHYHTNKPGTYVSAINGIEKETHKSKTHNM
                     YSNNTNHRAKDSTPDYHKESFKTSEVPSAIFGTMKPKKLENGRIPVSKPSEKVESDKQ
                     KYDKYVAKTQTSQNKQLEQEKQNDSVVKQGTASKSSDENVSSTTKSMPNYSKVDNTIK
                     IENIYASQIVEEIRRERERKVLQKRRFKKALQQKREEHKNEEQDAIQRAIDEMYAKQA
                     ERYVGDSSLNDDSDLTDNSTDASQLHTNGIENETVSNDENKQASIQNEDTNDTHVDES
                     PYNYEEVSLNQVSTTKQLSDDEVTVSNVTSQHQSALQHNVEVNDKDELKNQSRLIADS
                     EEDGATNKEEYSGSQIDDAEFYELNDTEVDEDTTSNIEDNTNRNASEMHVDAPKTQEH
                     AVTESQVNNIDKTVDNEIELAPRHKKDDQTNLNVNSLKTNDVNDGHVVEDSSMNEIEK
                     HNAEITENVQNEAAESEQNVEEKTIENVNPKKQTEKVSTLSKRPFNVVMTPSDKKRMM
                     DRKKHSKVNVPELKPVQSKQAVSESKPASQAAPSSRSDSQESNTNAYKTNNMTSNNVE
                     NNQLIGHAETENDYQNAQQYSEQKPSADSTQTEIFEESQDDNQLENEQVDQSTSSSVS
                     EVSDITEESEETTHPNNTSGQQDNDDQQKDLQPSFSNQNEDTANENRPRTNQQDVATN
                     QAVQTSKPMIRKGPNIKLPSVSLLEEPQVIESDEDWITDKKKELNDALFYFNVPAEVQ
                     DVTEGPSVTRFELSVEKGVKVSRITALQDDIKMALAAKDIRIEAPIPGTSRVGIEVPN
                     QNPTTVNLRSIIESPSFKNAESKLTVAMGYRINNEPLLMDIAKTPHALIAGATGSGKS
                     VCINSILMSLLYKNHPEELRLLLIDPKMVELAPYNGLPHLVAPVITDVKAATQSLKWA
                     VEEMERRYKLFAHYHVRNITAFNKKAPYDERMPKIVIVIDELADLMMMAPQEVEQSIA
                     RIAQKARACGIHMLVATQRPSVNVITGLIKANIPTRIAFMVSSSVDSRTILDSGGAER
                     LLGYGDMLYLGSGMNKPIRVQGTFVSDDEIDDVVDFIKQQREPDYLFEEKELLKKTQT
                     QSQDELFDDVCAFMVNEGHISTSLIQRHFQIGYNRAARIIDQLEQLGYVSSANGSKPR
                     DVYVTEADLNKE"
     gene            10530..11843
                     /locus_tag="NBI57_001152"
     CDS             10530..11843
                     /locus_tag="NBI57_001152"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HCD1822112.1"
                     /translation="MTHYHFVGIKGSGMSSLAQIMHDLGHEVQGSDIENYVFTEVALR
                     NKGIKILPFDANNIKEDMVVIQGNAFASSHEEIVRAHQLKLDVVSYNDFLGQIIDQYT
                     SVAVTGAHGKTSTTGLLSHVMNGDKKTSFLIGDGTGMGLPESDYFAFEACEYRRHFLS
                     YKPDYAIMTNIDFDHPDYFKDINDVFDAFQEMAHNVKKGIIAWGDDEHLRKIEADVPI
                     YYYGFKDSDDIYAQNIQITDKGTAFDVYVDGEFYDHFLSPQYGDHTVLNALAVIAISY
                     LEKLDVTNIKEALETFGGVKRRFNETTIANQVIVDDYAHHPREISATIETARKKYPHK
                     EVVAVFQPHTFSRTQAFLNEFAESLSKADRVFLCEIFGSIRENTGALTIQDLIDKIEG
                     ASLINEDSINVLEQFDNAVVLFMGAGDIQKLQNAYLDKLGMKNAF"
     gene            11917..12408
                     /locus_tag="NBI57_001153"
     CDS             11917..12408
                     /locus_tag="NBI57_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HCD1822113.1"
                     /translation="MDWILPIAGIIAAIAFLILCIGIVAVLNSVKKNLDYVAKTLDGV
                     EGQVQGITRETTDLLHKVNRLTEDIQGKVDRLNSVVDAVKGIGDSVQTLNSSVDRVTN
                     SITHNISQNEDKISQVVQWSNVAMEIADKWQNRHYRRGSANYKANNVATDANHSYTSR
                     VDK"
     gene            12482..13840
                     /locus_tag="NBI57_001154"
     CDS             12482..13840
                     /locus_tag="NBI57_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000193894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1822114.1"
                     /translation="MTQQQNDKRTLKNKHTYQNEPLPNRKDFVVSFITGALVGSALGL
                     YFKNKVYQKADDLKVKEQELSQKFEERKTQLEETVAYTKERVEGFLNKSKNEQAALKA
                     QQAAIKEEASANNLSDTSQEAQEIQEAKREAQAEADKSVAVSNEESKASALKVQQAAI
                     KEEASANNLSDTSQEAQEIQEAKKEAQAETDKSAAVSNEEPKAVALKAQQAAIKEEAS
                     ANNLSDTSQEAQEVQEAKKEAQAEKDSDTSTKDASAAKVEVSKPESQAERLANAAKQK
                     QAKLTPGSKESQLTEALFAEKPVAKNDLKEIPQLVTKKNDVSETETVNIDNKDTVKQK
                     EAKFENGVITRKADEKTTNNTAVDKKSGKQSKKTTPSNKRNASKASTNKTSGQKKQHN
                     KKSSQGAKKQSSSSKLTQKNNQTSNKNSKTTNAKSSNASKTPNAKVEKANSKIEKRTF
                     ND"
     gene            13996..14079
                     /locus_tag="NBI57_001155"
     CDS             13996..14079
                     /locus_tag="NBI57_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1822115.1"
                     /translation="MLFETSLDNNSYIAHFKLKMEKYSIIK"
BASE COUNT         5526 a         1945 c         2667 g         4159 t
ORIGIN      
        1 ttactaagta cgtaaatgtt acagttcata aatttatgct aaccatgaaa atgggatagc
       61 aattattttg aaaactagag tttatcgcct acagagattc aaaaacatgt agttttagac
      121 acggtaacat tatatgtcaa gtaagtgact atagaatgta tggtgtcaat gacagacatt
      181 acgatttaaa attagtcgtg taatgtaggc attggttgag gatttacaat aattatctgg
      241 acataatatt tgtaccagta tggtgttata aattagaata tatggctctt gaaaaaccta
      301 tttctagagc cattttctaa ttgaaagtga ggtggacgag ttggagcagt tttatcaatt
      361 agggtggaca cttgattcag caggtggtgc atctggtgaa gcatatatgg ctgaacaaga
      421 tggacaaaag ttgtttttaa aacgaaattc aaatccattt attgcggcat tatcagcaga
      481 aggtattgtg cccaaattag tatggacgaa acgcatagaa acaggcgagg ttgttacagc
      541 acaacattgg aaaaatgggc gtgaactatc ttcaaacgaa atgaagcaaa caagagttgc
      601 acatttatta aagaagatac acaattctag acctttatta agtatgttaa agcgtatgga
      661 aatggaacct attactcctg agattatgct taataaaatt aatgcctctt tatcaagaga
      721 agttttaaca catcatattg tgagaaaatc attaacctat ttagaagagc atataccgag
      781 tttagattcg cgttttttca ctgttgtaca tggtgatgtg aatcataata attggttatt
      841 atccgatcga gatgaactat ttttagttga ttgggaaggt gcaatgattg cagacccggc
      901 aattgatatc ggaatgctgc tttacaacta tgttccacaa caacaatggt ctgaatggct
      961 tgaaacatat ggtgttcaag agagcttgaa tttaaataaa cgtatgaaat ggtatacggt
     1021 aattcaatct ataggtcttg ttcaatggta tgaagagcaa aaaagatata aggatatgaa
     1081 cacttggctt aaattcctaa atgaagttat gaatagtaac atgtttattt aagaggagaa
     1141 taaaatatga gagttcgata caaaccatgg gctgaagatt atttaaaaga tcatcctgaa
     1201 ttggttgaca tggaaggtca acatgcaggg aaaatgactg aatggtttga taaaacgcaa
     1261 ccgatacaca ttgaaattgg ttcgggtatg gggcagttta ttacaacatt agctgctcaa
     1321 aatcctcata ttaactatat ttcaatggaa cgtgagaaaa gtatcgttta taaagtattg
     1381 gataaagtta aagagatggg tttaacaaat ttaaagatta tttgtaatga cgccattgaa
     1441 ttaaatgaat attttaaaga cggcgaagtt tcgcgtattt atttgaactt ttcagatcca
     1501 tggccaaaaa atcgtcatgc aaaacgacgt ttaacgtatc atacgttttt agcgttgtat
     1561 caacaaatct taaatgatga aggagattta cattttaaaa ctgataacag aggactattt
     1621 gcctacagtt tagaaagtat gtctcaattt ggaatgtatt ttacaaaaat aaatctaaat
     1681 ttacatcaag aagacgatgg cagtaatatt ttaacagaat atgaaaagaa attttcggat
     1741 aaagggtcac gtatttatcg tatggaagcg aaattccatt cacaaaaata aaacacttat
     1801 gagggtatgt acacatacaa tcataagtgt tttttcgtac tataatggtt ctatagataa
     1861 aggttctata ataaatcgga ctgatggaaa aaagtatttt acttttcatc tgtccgattt
     1921 tttgattttg aatataaaaa aagcgcaatt atctctataa ttaaaagtga ctaaacaaat
     1981 aactaaagga gataatgcgc ctataaacaa tgttatacta aatacctttg atttaaaaga
     2041 ataaagcttg cattttactt gtaaatttta attattaatt taaaattaca ggccgatggt
     2101 tatgatgcat ttacatataa tatgattgca ttaatcttta taagtagtta gaagtgtatt
     2161 agtgtggggc gtttcgtttt tgttcgcatt tatgaatagt gatatgatgt aataaataca
     2221 tgctaattgt aataacgaat acagaaagaa taggtgatgt tatgaagatc ggggatatat
     2281 ctattcatta tctaaatggt ggcaatacaa aaatggatgg cggtgcaatg tttggtgttg
     2341 ttccgaagcc gttgtggtca aagcaataca atgcaaatga acgaaatcaa atcaatttac
     2401 cgacacatcc aattttgatt caaacggctc aatataattt gattatagat gcgggtattg
     2461 gtaatggtaa attatctgaa aagcaattac gtaattttgg agtagatgaa gaaagtcata
     2521 taattgctga tttggcaaat tataatttaa cgccaaagga tattgattat gtgctaatga
     2581 cacatatgca ttttgatcat gctgccggtt tgactgatca agcgggacat gcaatttttg
     2641 aaaatgcgat tcatgttgtg caacaagatg agtggcatga gtttattgca cctaatataa
     2701 gaagtaaatc aacttactgg gataagaata aaggcgatta tagtaacaag ttgattttat
     2761 tcgaaaaaca ttttgaaccg gttccgggta tcaagatgca acatagtgga ggtcatagct
     2821 ttggccacac gattattacg attgaaagtc aaggagataa agcagttcat atgggtgata
     2881 tattcccaac tactgcacat aaaaatcctc tatgggtaac ggcatatgat gattatccta
     2941 tgcaatcgat tcgtgaaaaa gaacgcatga taccatattt tattcagcaa caatattggt
     3001 tcttgtttta tcatgatgaa aactactttg ctgtaaaata cagcgatgat ggtgaaaaca
     3061 tagatgcata tattttacgt gaaacattag ttgataataa ctaaaataaa gatgtattac
     3121 taaacaaatt ttcaaaaata aaaaaatgag ccacatccaa tcttactaat tagggtgtgg
     3181 ctcattttta agttttacga tccaaatcaa atatggataa aattcgtatt aacgctctac
     3241 aatgttaatg acttcaccag tatatgcatc tgcataaaaa tcataatgaa tattttgacc
     3301 atttttaata gttgtaattc caccttgata aactaaacgg tatttatcag tttcaggatg
     3361 aacgaatggt tcataaacga tatatgagcc tttgacattc ataaaatatg attttacttc
     3421 attaagtact ttaacgggat tatagcgttt acgattaata aattggatga ttgaaatggt
     3481 agaaataatt acaatggcta ctgcaattat tgttggaatt atatatttca gtttagtcat
     3541 catttatgct cccttgattt caaattcata tcattagttt accatattga agatgatata
     3601 ataacttata aggagtgaga acttttatga acataaataa aaaagtaaca ttacaacgaa
     3661 ttcaaacttt aactgagctt catggggcac caggttttga agaagaagta aaaaattata
     3721 tgactcagca aatggcgccg tatgtagatg aatttattga aaatcgtatg ggtggatttt
     3781 ttggtgtgaa aaaatctaaa aatccaaatg caaaacgtgt aatgattgca gcacatatgg
     3841 atgaaatcgg atttatgatt acaaatatca ctaaaaatgg aatgattcaa ttcacaaatt
     3901 taggtggtgt tgcaaatgat atttggcaag gacaacgctt agtaattaaa aatagaaatg
     3961 gcgataaaat tatcggtgtt gtttctaata tacctaaaca ttttcgtact ggtagtgaag
     4021 gtgcaccgga aattaaagat ttaacattag atataggtgc tcaaaatgaa gatgaggtgc
     4081 gtgagcgcgg aatagatata ggagatacaa ttgtacctca cacgccattc acacagttat
     4141 ctgaacatcg atatagtgct aaagcatggg ataatcgtta tggttgtgtc ttggcaattg
     4201 aaatactaga attattaaaa gatatagaat tagatgtaga cttgtatgtt ggcgcaaatg
     4261 ttcaagaaga ggttggatta cgaggtgcga aagcatctgc agagatgata gacccagacg
     4321 ttgcatttgt agtcgattgt tcacctgcca atgacgttaa aggaaaccaa ccattatctg
     4381 gtgaacttgg taaagggacg ttaattcgca taaaagacgg tacaatgatt ttaaagcctg
     4441 tatttagaga ttatttatta aagttagtag aagcacatga cattgaacat caatactata
     4501 tgtcaccagg tggaacagat ggtggagaaa ttcataaagc taatattggt attccgactg
     4561 cagttattgg tgtatgtgca cgatatattc atagtacaga ctcagtattt gatataagag
     4621 actattttgc agctagatct ttactttcag aagccatttg taatttagat aataatcaaa
     4681 tagaaacatt acaatataaa taatcgggta ataacaacta ttatctctaa atagttatat
     4741 ataatcatta attaaggaga cataaaaatg aaacagcttg aatcagaaca acaatttgaa
     4801 tctttaaaac aaggtgctac agtatttgaa ttcactgcag gctggtgtcc agattgtaga
     4861 gtgatagaac cagatttacc ggaattagaa gcgagatatc ctatgtttga cttcgtatca
     4921 gtagaccgtg acaaatttat ggatatttgt attgaaaatg gtattatggg gattccaagt
     4981 tttctagtat ataaaaatgg tgaactgctt ggaagttata ttggaaaaga acgaaaatca
     5041 attgaacaga tagatgcatt tttagctcaa tatgtgtaat ttagactaga gaaaaacggg
     5101 gtaatactcg tttttctctg ttactatgtg ttgatttatt gtaaactatt ataaggtgcg
     5161 aaattaggag tgttacatat gaataccttt caaatgagag ataaattaaa ggaacgttta
     5221 agccatttag acgttgattt taaatttaat cgtgaagaag aaactttgcg tatttatcga
     5281 acagataata acaaaggtat cacgattaaa cttaacgcta tagtcgcaaa atatgaagat
     5341 aaaaaagaaa aaattgtaga tgaaattgtt tattacgttg atgaagctat tgcacaaatg
     5401 gcagataaaa cccttgagag tatatcatct agtcaaatta tgcctgtcat tagagcgact
     5461 agctttgata aaaaaactaa acaaggtgtt ccttttatct atgatgagca tactgcagaa
     5521 acagcagttt attatgcagt cgatttaggg aaatcatatc gtctaattga cgaaagcatg
     5581 ttagaggatt tgaagttaac tgaacaacaa ataagagaaa tgtctctgtt taatgttaga
     5641 aaattgtcaa attcatatac gactgatgaa gtaaaaggta atatttttta ttttattaac
     5701 tcaaatgacg ggtatgatgc aagtaggata ctaaatactg catttttaaa tgaaattgag
     5761 gcacaatgtc aaggcgaaat gctcgtagca gtgccacacc aagatgtgtt aattattgca
     5821 gatatacgca ataaaacagg atatgatgtg atggcacatt taacaatgga atttttcact
     5881 aaaggtctag ttccaattac atcattatcc tttggatata aacagggtca tcttgaaccg
     5941 atatttattt taggtaaaaa taataaacaa aaaagagatc caaacgtgat tcagcgttta
     6001 gaagcaaatc gtcgtaaatt taataaagat aaatagaaat aattggataa ggagttttgt
     6061 cataatgaat ttattttaca atcctaaata tgtaggagat gtcgcatttt tacaaattga
     6121 accagttgaa ggtgaattaa actacaataa aaaaggtaat gttgttgaaa ttactaatga
     6181 aggtaatgtt gtaggttata atatttttga aatttcaaaa gatataacaa ttgaagaaaa
     6241 aggtcatatt aaattaactg atgaacttgt aaatgtattc caaaagcgta tttcagaagc
     6301 tggttttgat tataaattaa atgctgatct atcaccgaaa tttgtagttg gctacgttga
     6361 aactaaagac aaacatcctg atgcagataa attaagtgta ctaaatgtaa acgttggaaa
     6421 tgacacatta caaattgtat gtggcgcgcc taacgttgaa gctggacaga aagttgttgt
     6481 tgctaaagta ggtgcagtga tgcctagcgg tatggtaatt aaagatgctg aattacgtgg
     6541 tgttgcctca agcggtatga tttgttcaat gaaagaattg aatttaccta atgcacctga
     6601 agaaaaaggt attatggtat taaatgacag ctatgaaatt ggacaagcat tttttgaata
     6661 attaaggaag gtagtgaaaa tatgagctgg tttgataaat tattcggcga agataatgat
     6721 tcaaatgatg acttgattca tagaaagaaa aaaagacgtc aagaatcaca aaatatagat
     6781 aacgatcatg actcattact gcctcaaaat aatgatattt atagtcgtcc gaggggaaaa
     6841 ttccgttttc ctatgagcgt agcttatgaa aatgaaaatg ttgaacaatc tgcagatact
     6901 atttcagatg aaaaagaaca ataccatcga gactatcgca aacaaagcca cgattctcgt
     6961 tcacaaaaac gacatcgccg tagaagaaat caaacaactg aagaacaaaa ttatagtgaa
     7021 caacgtggga attctaaaat atcacagcaa agtataaaat ataaagatca ttcacattac
     7081 catacgaata agccaggtac atatgtttct gcaattaatg gtattgagaa ggaaacgcac
     7141 aagtcaaaaa cacataatat gtattctaat aatacaaatc atcgtgctaa agattcaact
     7201 ccagattatc acaaagaaag tttcaagact tcagaggtac cgtcagctat ttttggcaca
     7261 atgaaaccta aaaagttaga aaatggtcgt atccctgtaa gtaaaccttc agaaaaagtt
     7321 gagtcagata aacaaaaata tgataaatat gtagctaaga cgcaaacgtc tcaaaataaa
     7381 caattagaac aagaaaaaca aaatgatagt gttgtcaaac aaggaactgc atctaaatca
     7441 tctgatgaaa atgtatcatc aacaacaaaa tcaatgccta attattcaaa agttgataat
     7501 actatcaaaa ttgaaaatat ttatgcttca caaattgttg aagaaattag acgtgaacga
     7561 gaacgtaaag tgcttcaaaa gcgtcgattt aaaaaagcgt tgcaacaaaa gcgtgaagaa
     7621 cataaaaacg aagagcaaga tgcaatacaa cgtgcaattg atgaaatgta tgctaaacaa
     7681 gcggaacgct atgttggtga tagttcatta aatgatgata gtgacttaac agataatagt
     7741 acagatgcta gtcagcttca tacaaatggc atagagaatg aaactgtatc aaatgatgaa
     7801 aataaacaag cgtcaataca aaatgaagac actaatgaca ctcatgtaga tgaaagtcca
     7861 tacaattatg aggaagttag tttgaatcaa gtatcgacaa caaaacaatt gtcagatgat
     7921 gaagttacgg tttcgaatgt aacgtctcaa catcaatcag cactacaaca taacgttgaa
     7981 gtaaatgata aagatgaact aaaaaatcaa tccagattaa ttgctgattc agaagaagat
     8041 ggagcaacga ataaagaaga atattcagga agtcaaatcg atgatgcaga attttatgaa
     8101 ttaaatgata cagaagtaga tgaggatact acttcaaata tcgaagataa taccaataga
     8161 aacgcgtctg aaatgcatgt agacgctcct aaaacgcaag agcacgcagt aactgaatct
     8221 caagttaata atatcgataa aacggttgat aatgaaattg aattagcacc gcgtcataaa
     8281 aaagatgacc aaacaaactt aaatgtcaac tcattgaaaa cgaatgatgt gaatgatggt
     8341 catgttgtgg aagattcaag catgaatgaa atagaaaagc ataacgcaga aattacagaa
     8401 aatgtgcaaa acgaagcagc tgaaagtgaa caaaatgtcg aagagaaaac tattgaaaac
     8461 gtaaatccaa agaaacagac tgaaaaggtt tcaactttaa gtaaaagacc atttaatgtt
     8521 gtcatgacgc catctgataa aaagcgtatg atggatcgta aaaagcattc aaaagtcaat
     8581 gtgcctgaat taaagcctgt acaaagtaaa caagctgtga gtgaaagcaa gcctgcgagt
     8641 caagccgcac catcatcaag atctgattca caagagtcaa acacaaatgc atataaaaca
     8701 aataatatga catcaaacaa tgttgagaac aatcaactta ttggtcatgc agaaacagaa
     8761 aatgattatc aaaatgcaca acaatattca gagcagaaac cttctgctga ttcaactcaa
     8821 acggaaatat ttgaagaaag ccaagatgat aatcaattgg aaaatgagca agttgatcaa
     8881 tcaacttcgt cttcagtttc agaagtaagc gacataactg aagaaagcga agaaacaaca
     8941 catccaaaca atactagtgg acaacaagat aatgatgatc aacaaaaaga tttacagcct
     9001 tcattttcaa atcaaaatga agatacagct aatgaaaata gacctcggac gaaccaacaa
     9061 gatgttgcaa caaatcaagc tgtacaaaca tctaagccga tgattcgtaa aggcccaaat
     9121 attaaattgc caagtgtttc attactagaa gaaccacaag ttattgagtc ggacgaggac
     9181 tggattacag ataaaaagaa agaactgaat gacgcattat tttactttaa tgtacctgca
     9241 gaagtacaag atgtaactga aggtccaagt gttacaagat ttgaattatc agttgaaaaa
     9301 ggtgttaaag tttcaagaat tacggcatta caagatgaca ttaaaatggc attggcagcg
     9361 aaagatattc gtatagaagc gcctattcca ggaactagtc gtgttggtat tgaagttccg
     9421 aaccaaaatc caacgacagt caacttacgt tctattattg aatctccaag ttttaaaaat
     9481 gctgaatcta aattaacagt tgcgatgggg tatagaatta ataatgaacc attacttatg
     9541 gatattgcta aaacgccaca cgcactaatt gcaggtgcaa ctggatcagg gaaatcagtt
     9601 tgtatcaata gtattttgat gtctttacta tataaaaatc accctgagga attaagatta
     9661 ttacttatcg atccaaaaat ggttgaatta gctccttata atggtttgcc acatttagtt
     9721 gcaccggtaa ttacagatgt caaagcagct acacagagtt taaaatgggc cgtagaagaa
     9781 atggaacgac gttataagtt atttgcacat taccatgtac gtaatataac agcatttaac
     9841 aaaaaagcac catatgatga aagaatgcca aaaattgtca ttgtaattga tgagttggct
     9901 gatttaatga tgatggctcc gcaagaagtt gagcagtcta ttgctagaat tgctcaaaaa
     9961 gcaagagcat gtggtattca tatgttagta gctacgcaaa gaccatctgt caatgtaatt
    10021 acaggtttaa ttaaagccaa cataccaaca agaattgcat ttatggtatc atcaagtgta
    10081 gattcgagaa cgatattaga cagtggtgga gcagaacgct tgttaggata tggcgatatg
    10141 ttatatcttg gtagcggtat gaataaaccg attagagttc aaggtacatt tgtttctgat
    10201 gacgaaattg atgatgttgt tgattttatc aaacaacaaa gagaaccgga ctatctattt
    10261 gaagaaaaag aattgttgaa aaaaacacaa acacaatcac aagatgaatt atttgatgat
    10321 gtttgtgcat ttatggttaa tgaaggacat atttcaacat cattaatcca aagacatttc
    10381 caaattggct ataatagagc agcaagaatt atcgatcaat tagagcaact cggttatgtt
    10441 tcgagtgcta atggttcaaa accaagggat gtttatgtta cggaagcaga tttaaataaa
    10501 gaataattat gagtaaggag ttttatataa tgacacacta tcattttgtc ggaattaaag
    10561 gttctggcat gagttcatta gcacaaatca tgcatgattt aggacatgaa gttcaaggat
    10621 cggatattga gaactacgta tttacagaag ttgctcttag aaataagggg ataaaaatat
    10681 taccatttga tgctaataac ataaaagaag atatggtagt tatacaaggt aatgcattcg
    10741 cgagtagcca tgaagaaata gtacgtgcac atcaattgaa attagatgtt gtaagttata
    10801 atgatttttt aggacagatt attgatcaat atacttcagt agctgtaact ggtgcacatg
    10861 gtaaaacttc tacaacaggt ttattatcac atgttatgaa tggtgataaa aagacttcat
    10921 ttttaattgg tgatggcaca ggtatgggat tgcctgaaag tgattatttc gcttttgagg
    10981 catgtgaata tagacgtcac tttttaagtt ataaacctga ttacgcaatt atgacaaata
    11041 ttgatttcga tcatcctgat tattttaaag atattaatga tgtttttgat gcattccaag
    11101 aaatggcaca taatgttaaa aaaggtatta ttgcttgggg tgatgatgaa catctacgta
    11161 aaattgaagc agatgttcca atttattatt atggatttaa agattcggat gacatttatg
    11221 ctcaaaatat tcaaattacg gataaaggta ctgcttttga tgtgtatgtg gatggtgagt
    11281 tttatgatca cttcctgtct ccacaatatg gtgaccatac agttttaaat gcattagctg
    11341 taattgcgat tagttattta gagaagctag atgttacaaa tattaaagaa gcattagaaa
    11401 cgtttggtgg tgttaaacgt cgtttcaatg aaactacaat tgcaaatcaa gttattgtag
    11461 atgattatgc acaccatcca agagaaatta gtgctacaat tgaaacagca cgaaagaaat
    11521 atccacataa agaagttgtt gcagtatttc aaccacacac tttctctaga acacaagcat
    11581 ttttaaatga atttgcagaa agtttaagta aagcagatcg tgtattctta tgtgaaattt
    11641 ttggatcaat tagagaaaat actggcgcat taacgataca agatttaatt gataaaattg
    11701 aaggtgcatc gttaattaat gaagattcta ttaatgtatt agaacaattt gataatgctg
    11761 ttgttttatt tatgggtgca ggtgatattc aaaaattaca aaatgcatat ttagataaat
    11821 taggcatgaa aaatgcgttt taatatgttt ataatagagt agtatgggta tttattatta
    11881 atgacattat tacatgttaa ttaggaggcg tttttaatgg attggatttt accaattgct
    11941 ggaattatcg ctgcgattgc attcttaatt ttatgtatcg gtatcgtagc tgtattaaat
    12001 tctgttaaga aaaacttaga ttatgttgca aaaacacttg acggtgtaga aggtcaagtt
    12061 caaggtatta ctcgtgaaac aacagattta cttcataaag taaaccgttt aactgaggat
    12121 atccaaggta aagtagatcg tttaaactca gttgtagatg ctgttaaagg tatcggtgac
    12181 tcagtacaaa cgttaaacag ctctgtagat cgtgtaacaa attcaattac acataatatt
    12241 tctcaaaatg aagataaaat ctcacaagtt gttcaatggt caaatgttgc aatggaaatt
    12301 gcagacaaat ggcaaaatag acactaccgt cgtggaagtg caaattacaa agctaataat
    12361 gtagcaactg atgcaaatca tagctatact tctagagtag ataaataatt aataattaaa
    12421 caaatgcact taaatgaggt tgttacttat ttaagtgcat ttgttatagg aggatataca
    12481 aatgacgcag caacaaaatg ataaaagaac attaaaaaat aaacacactt atcaaaatga
    12541 accactacca aaccgtaaag attttgttgt tagttttata actggcgcgc ttgttggttc
    12601 agctttaggt ttgtatttta aaaataaagt ttatcaaaaa gcagatgatt taaaagtcaa
    12661 agaacaagaa ctgtcgcaaa agtttgaaga aagaaaaacg caacttgaag aaacggttgc
    12721 ctatacaaaa gaacgtgttg aaggattttt aaacaaatct aaaaatgagc aagcggcatt
    12781 aaaggcacaa caagcagcga taaaagaaga agcaagtgca aataatttaa gtgatacatc
    12841 acaagaagca caagagattc aagaagctaa aagagaagca caagcagaag cggataaaag
    12901 tgtggctgta tcaaatgaag agtcaaaagc atcggcatta aaagtacaac aagcagcaat
    12961 aaaagaagaa gcaagtgcaa ataatttaag tgacacgtca caagaggcac aagagattca
    13021 agaagctaaa aaagaagcac aagcagaaac agataaaagt gcagctgtat caaatgaaga
    13081 accaaaagca gtggcattga aagcacaaca agcagcgata aaagaagaag caagtgcaaa
    13141 taatttaagt gatacatcac aagaggcaca agaggttcaa gaagctaaaa aagaagcaca
    13201 agcagagaaa gacagtgaca catcaactaa agatgcaagt gcagcaaagg tagaagtatc
    13261 aaaaccagag tcacaagctg aaagattagc aaacgctgca aaacagaagc aagctaaatt
    13321 aacaccaggt tcaaaagaga gtcaattaac tgaagcgtta tttgcagaaa aaccagttgc
    13381 taaaaatgac ttgaaagaaa ttcctcaatt agttactaaa aagaatgatg tatcagagac
    13441 agagacggtt aatatagata ataaagacac tgttaaacaa aaagaagcta aatttgaaaa
    13501 tggtgttatt acacgtaaag ctgatgaaaa aacaactaat aatacagctg ttgacaagaa
    13561 atcaggtaaa caatctaaaa aaacaacacc ttcaaataaa cgaaatgcat caaaagcatc
    13621 tacaaataaa acttcaggtc agaaaaagca acataataag aaatcatcac aaggtgcaaa
    13681 gaaacaaagt agttcaagta agttaactca aaagaataat caaactagta ataagaattc
    13741 aaaaacaaca aatgctaaat catccaatgc atcaaaaacg ccaaatgcta aagttgaaaa
    13801 agctaatagt aaaatagaga aacgtacatt caatgactaa gattactaaa atcgttgaat
    13861 aatcaatcat agcagtaagc tatgtattat atttttaaaa attgaatttc aaaataaaag
    13921 gtaggcattc aggtgcttac cttttttgat atcatcagtt tattttgaaa ggcaacgcga
    13981 tcatttcatg tatttatgct gtttgaaaca tcgttagata acaatagtta tattgcacat
    14041 tttaagctga agatggaaaa atattcgata atcaaataaa caatgaatct tagaaggtac
    14101 aatgacgttc actaattcaa atatagccgc tcatgttggt gagtgatgtt cactatagat
    14161 ttatattaat atacaaaggt tgttaatttt tattaagcgt taggttgaat gtatgagaat
    14221 tttagattta taatagaaga tagaaacgaa aatttttctt aaaagcagta atgttgactc
    14281 aaaataagct ataataa
//
LOCUS       DAHHSC010000051        14205 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.051, whole genome shotgun sequence.
ACCESSION   DAHHSC010000051 DAHHSC010000000
VERSION     DAHHSC010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 14205)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14205)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14205
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.051"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            complement(17..1213)
                     /locus_tag="NBI57_001156"
     CDS             complement(17..1213)
                     /locus_tag="NBI57_001156"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000933820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HCD1822116.1"
                     /translation="MLNNKRLFTSESVTEGHPDKIADQVSDAILDAILKDDPNARVAC
                     ETTVTTGMALIAGEISTTTYVDIPKVVRETIKEIGYTRAKYGYDYETMAILTAIDEQS
                     PDIAQGVDKALEYRDKDSEEEIEATGAGDQGLMFGYATNETETYMPLAIYLSHQLAKR
                     LSDVRKDGTLNYLRPDGKVQVTVEYDENDNPVRIDTIVVSTQHADDVTLEQIQEDIKA
                     HVIYPTVPENLINEQTKFYINPTGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGCF
                     SGKDPTKVDRSAAYAARYVAKNIVAAGLADQCEVQLAYAIGVAEPVSIAIDTFGTGKV
                     SEGQLVEAVRKHFDLRPAGIIKMLDLKQPIYKQTAAYGHFGRTDVLFPWEKLDKVEEL
                     KDAVKY"
     regulatory      complement(1319..1430)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            1585..3177
                     /gene="pckA"
                     /locus_tag="NBI57_001157"
     CDS             1585..3177
                     /gene="pckA"
                     /locus_tag="NBI57_001157"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HCD1822117.1"
                     /translation="MSVDTYTETTKIDKLLKKPTSHFQLSTTQLYNKILDNNEGVLTE
                     LGAVNASTGKYTGRSPKDKFFVSEPSYRDNIDWGEINQPIDEETFLKLYHKVLDYLDK
                     KDELYVFKGYAGSDKDTMLKLTVINELAWHNLFAKNMFIRPESKEEATKIKPNFTIVS
                     APHFKADPEVDGTKSETFVIISFKHKVILIGGTEYAGEMKKGIFSVMNYLLPMQDIMS
                     MHCSANVGEKGDVALFFGLSGTGKTTLSADPHRKLIGDDEHGWNKNGVFNIEGGCYAK
                     AINLSKEKEPQIFDAIKYGAILENTVVAEDGSVDFEDNRYTENTRAAYPINHIDNIVV
                     PSKAAHPNTIIFLTADAFGVIPPISKLNKDQAMYHFLSGFTSKLAGTERGVTEPEPSF
                     STCFGAPFFPLHPTVYADLLGELIDLHDVDVYLVNTGWTGGKYGVGRRISLHYTRQMV
                     NQAISGKLKNAEYTKDSTFGLSIPVEIEDVPKTILNPINAWSDKEKYKAQAEDLIQRF
                     EKNFEKFGEKVEHIAEKGSFNK"
     gene            complement(3555..4301)
                     /locus_tag="NBI57_001158"
     CDS             complement(3555..4301)
                     /locus_tag="NBI57_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HCD1822118.1"
                     /translation="MPIESFTHQFEEITYLSDDLQVKALMMTPHHEVNRIVVYLRGGK
                     GQVGRVRAGRLMQFSDSQTLVIGPYYRGNNGSEGKDEFYRGDLNDVTQLLRLLHDKYP
                     HAFIHMVGFSRGGLQGLLTFQDLPVTSYTIWGGVSDIDLMYEERVDLRGMLRRMIGHP
                     KKDRAAYEARQAIPNINENSPPILIVHGGKDQQVGIHHAYYLADQLELKGATHETFYQ
                     MAEGHVPRPPAMVETLTYIKEFMKQVELHS"
     gene            complement(4306..4779)
                     /gene="ytkD"
                     /locus_tag="NBI57_001159"
     CDS             complement(4306..4779)
                     /gene="ytkD"
                     /locus_tag="NBI57_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphatase YtkD"
                     /protein_id="HCD1822119.1"
                     /translation="MKFRDKDNRQVNLTFKKDNEIADGNHVLAIPTFKNQLLFTKHNL
                     RGIEFPGGKRERGESSAEAVTRELYEETGAKVKNIHYIAQYTIETHDQTDFVKDVYFI
                     EVESLVSKNDYLETAGPVLFNCINDIELAQRSFLLQDSTILKCVERVQSLGFYQT"
     gene            4845..5102
                     /gene="yidD"
                     /locus_tag="NBI57_001160"
     CDS             4845..5102
                     /gene="yidD"
                     /locus_tag="NBI57_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HCD1822120.1"
                     /translation="MKKIFLAMIHFYQRFISPLTPPTCRFYPTCSEYTREAIQYHGAF
                     KGLYLGIRRILKCHPLHKGGFDPVPLKKDKSASKHSHKHNH"
     gene            complement(5099..6100)
                     /gene="menC"
                     /locus_tag="NBI57_001161"
     CDS             complement(5099..6100)
                     /gene="menC"
                     /locus_tag="NBI57_001161"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HCD1822121.1"
                     /translation="MKLTALHFYKYSEPFKSQIVTPKVTLTHRDCLFIELIDDKGNAY
                     FGECNAFQTDWYDHETIASVKHVIEQWFEDNRNKSFETYEAALKLVDSLENTPAARAT
                     IVMALYQMFHVLPSFSVAYGATASGLSNKQLESLKATKPTRIKLKWTPQIMHQIRVLR
                     ELDFHFQLVIDANESLDRQDFTQLQLLAREQVLYIEEPFKDISMLDEVVDGTIPPIAL
                     DEKATSLLDIINLIELYNVKVVVLKPFRLGGIDKVQTAIDTLKSHGVKVVIGGMYEYG
                     LSRYFTAMLARKGDYPGDVTPAGYYFDQDVVTKSGILKEGRLEFRPPLVDITQLQPY"
     gene            complement(6105..7583)
                     /gene="menE"
                     /locus_tag="NBI57_001162"
     CDS             complement(6105..7583)
                     /gene="menE"
                     /locus_tag="NBI57_001162"
                     /EC_number="6.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate--CoA ligase"
                     /protein_id="HCD1822122.1"
                     /translation="MDFWLYKQAQQNGHHIAITDGQESYTYQNLYCEASLLAKRLKAY
                     QQSRVGLYIDNSIQSIILIHACWLANIEIAMINTRLTPNEMTNQMRSIDVQLIFCTLP
                     LELRGFQIVSLDDIEFAGRDITTNGLLDNTMGIQYDTSNETVVPKESPSNILNTSFNL
                     DDIASIMFTSGTTGPQKAVPQTFRNHYASAIGCKESLGFDRDTNWLSVLPIYHISGLS
                     VLLRAVIEGFTVRIVDKFNAEQILTMIKNERITHISLVPQTLNWLMQQGLHEPYNLQK
                     ILLGGAKLSATMIETALQYNLPIYNSFGMTETCSQFLTATPEMLHARPDTVGMPSANV
                     DVKIKNPNKEGHGELMIKGANVMNGYLYPTDLTGTFENGYFNTGDIAEIDHEGYVMIY
                     DRRKDLIISGGENIYPYQIETVAKQFPGISDAVCVGHPDDTWGQVPKLYFVSESDISK
                     AQLIAYLSKHLAKYKVPKHFEKVDTLPYTSTGKLQRNKLYRG"
     gene            complement(7742..8197)
                     /locus_tag="NBI57_001163"
     CDS             complement(7742..8197)
                     /locus_tag="NBI57_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4909 domain-containing protein"
                     /protein_id="HCD1822123.1"
                     /translation="MKICLMLYLLIFLSSCGTNNKIVGQRFEIQQHNETIGSIYFSAD
                     YAHIRGIEKGTAKYFIDKVGSKRYLFIEYIPDNVLNCKPDFWKTLKYKKDKVTYYVYL
                     IENLDDEVFHLSALQDMNRIPIDIADDVATMGKSPHQNDRMTLKLNKNN"
     gene            8500..9150
                     /locus_tag="NBI57_001164"
     CDS             8500..9150
                     /locus_tag="NBI57_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001795210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excalibur calcium-binding domain-containing
                     protein"
                     /protein_id="HCD1822124.1"
                     /translation="MKITYKYRGDLPLNTENNKNQNQSVKNSERRGMLKGCGGCLISF
                     ILLIILLSACSMMFSNNDNSTSNQSSKTQLTQKDENKNEDKPEEKSETATDEDLQSTE
                     EVPANENTENNQHEIDEITTKDQSDDEINTPNVAEDKSQDDLKDDLKEKQQSSNHHQS
                     TQPKTSPSTETNRQQSFANCKQLRQVYPNGVTADHPAYRPHLDRDKDNRACEPDKN"
     gene            9231..10226
                     /locus_tag="NBI57_001165"
     CDS             9231..10226
                     /locus_tag="NBI57_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4352 domain-containing protein"
                     /protein_id="HCD1822125.1"
                     /translation="MSNQFKSEEERRQWEQFQAFQNQQNQQHQQYGQKKSKKGWFWGC
                     GGCLVLFILIIIGISACTAGVTGNLGGNSSKEANKTHKIGETVKNGDLEVTVNSVETM
                     KSVGPSLAPTNAKGIFVVADVTIKNKGKEALTIDSSMFKLKSGDKTFEADNTGSMSAN
                     QSDNGSIENSFFLQRINPDSTAQGKIVFDVSENIANAKDKKLEVISSLFSVKKITFDL
                     SDAKKTSKAKKDKQDTEVAVASSNSDNVNYEASATTPATTSSADTDSEDSEKSSKDED
                     KQNASKSDKSSVEKSESNEETATVEPMPQSKPTTSEAPPSQNNHNENNMYDASTE"
     gene            10301..10927
                     /locus_tag="NBI57_001166"
     CDS             10301..10927
                     /locus_tag="NBI57_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1822126.1"
                     /translation="MKFKAIVAITLSFSLLTACGANQHKENNSKSNDTTKKTQETDNT
                     TQSNTEKQMTPQEAEDIVRNDYKARGANENQTLNYKTNLERSNEHEYYVEHLVRDAVG
                     TPLKRCAIVNRHNGTIINIFDDMSEKDKEEFEAFKKRSPKYNPGMSDHDKTDGESEDI
                     QHHDNDNNKAIQNDTPDQKAVDKNDKNAVNKEEKHDNHAKNSAETKVK"
     gene            10968..11309
                     /locus_tag="NBI57_001167"
     CDS             10968..11309
                     /locus_tag="NBI57_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3969 family protein"
                     /protein_id="HCD1822127.1"
                     /translation="MITFENIQQLEKYTLMTMHGLFNQLKLGIISIDNAEHTLFTPYM
                     MESLSSLGVKPDIIDLIHKGTELEDFAAFNLSIEDTVTVCLQRTEELLNQYKNVEFND
                     KILINWRIIQE"
     gene            11410..11982
                     /locus_tag="NBI57_001168"
     CDS             11410..11982
                     /locus_tag="NBI57_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000414203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1822128.1"
                     /translation="MEKNEYTAKYNEYSQLLDATYSQAVAYLLNKYGAVTDDYYKEKS
                     YTRFLNGEIKSISKGKYTRASEGLYCHHISEDKFQNLSDLRFISEFKYSYNVQKKENL
                     VYCDLIEHLILHAIITKESYGQFGVAGLCQMIKPTVIDWYISEYTPKPAWMQATKARA
                     YLPRILVEKLLIKIDDMLEGIEIYDFLESR"
     gene            complement(12180..13192)
                     /locus_tag="NBI57_001169"
                     /pseudo
     CDS             complement(12180..13192)
                     /locus_tag="NBI57_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611271.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            13337..13432
                     /gene="pepA1"
                     /locus_tag="NBI57_001170"
     CDS             13337..13432
                     /gene="pepA1"
                     /locus_tag="NBI57_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001801861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin
                     PepA1"
                     /protein_id="HCD1822129.1"
                     /translation="MMLIFVHIIAPVISGCAIAFFSYWLSRRNTK"
     gene            13555..13656
                     /locus_tag="NBI57_001171"
     CDS             13555..13656
                     /locus_tag="NBI57_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1822130.1"
                     /translation="MQGFKEKHQELKKALCQIGLMSSISEVRQLNIA"
     gene            complement(13634..13795)
                     /locus_tag="NBI57_001172"
                     /pseudo
     CDS             complement(13634..13795)
                     /locus_tag="NBI57_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001066885.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(13786..14190)
                     /locus_tag="NBI57_001173"
                     /pseudo
     CDS             complement(13786..14190)
                     /locus_tag="NBI57_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001824512.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         4710 a         2576 c         2242 g         4677 t
ORIGIN      
        1 ctatattaaa ttcatcttaa tattttactg cgtcttttaa ttcttccact ttgtctaatt
       61 tttcccatgg gaataataca tctgtacgtc caaaatgacc ataagcagca gtttgtttgt
      121 aaatcggttg tttcaaatca agcattttaa taatgcctgc aggtcttagg tcaaagtgtt
      181 ttctaactgc ttcgacaagt tgtccttcag aaactttacc tgttccaaat gtatcaattg
      241 caattgacac tggttctgca acaccaatcg catatgccaa ttgtacttca cattgatctg
      301 ctaaacctgc tgcaacaata tttttagcca cataacgtgc agcatatgca gctgagcggt
      361 ctacttttgt aggatcctta ccactgaagc atccgccacc atgacgtgca tagccaccat
      421 acgtatcaac aatgatttta cgtcctgtta atcctgcatc accttgaggt ccaccgatta
      481 caaagcgtcc tgtaggattg atgtagaatt tagtttgttc attaatcaag ttttctggaa
      541 cagttggata aatgacatgc gctttgatgt cttcttgaat ttgttcaagt gtcacatcat
      601 cagcatgttg tgttgatacg acaatcgtat caatacgtac tgggttatca ttttcatcat
      661 attcaacagt gacctgaact ttaccgtctg gtcgtaaata atttaacgta ccatctttac
      721 gcacatcaga taaacgtttt gccaattgat gtgataaata aattgctaga ggcatgtacg
      781 tttctgtttc attcgttgcg taaccaaaca ttaaaccttg gtcacctgca cctgttgctt
      841 caatttcttc ttcgctatct ttatcacgat actctaatgc tttatccacg ccttgtgcaa
      901 tgtcaggtga ttgttcatca atcgcagtta aaattgccat tgtttcataa tcataaccat
      961 attttgctct tgtgtatcca atttctttaa ttgtttctct aacaactttc ggaatatcaa
     1021 catatgttgt tgtagaaatt tcgccggcga tcaatgccat acctgttgta acagttgttt
     1081 cacaagctac acgtgcattt gggtcgtctt ttaaaatagc atctaatatt gcatctgaca
     1141 cttggtcagc gattttatct gggtgtcctt ctgtaacaga ctctgaagta aataatcgtt
     1201 tgttatttaa catagtttgc tcctttaaat ttatattacg aaaattctct ctctgtgagc
     1261 taaataaaaa agaccttcta actattaata tagagagaag gcctaatacg tccattcgct
     1321 cttatcgttc agacctattt gtctgcaaac ggtttggcac ctttctttta taaaaaaaga
     1381 ggttgctggg tttcattggg tccatgtccc tccaccactc aggataagag aatccgttaa
     1441 aaataatagt acctaattaa tgaattaatg tcaatttttc acaaataaat ttacagtaaa
     1501 atattgtaga ttaattatgt taatgtgtta tactaattaa atgtaaaggc ttacatttaa
     1561 attatcgctt tggagggatt taggatgtca gtagacacat acactgaaac aactaaaatt
     1621 gacaaattac tgaaaaaacc aacgtcacat tttcaacttt cgacgacaca actttataat
     1681 aaaatcttag acaataacga aggggtatta acagaacttg gtgctgttaa tgcaagtact
     1741 ggaaaatata ctggtcgttc gcctaaagac aaattttttg tctctgaacc ttcatataga
     1801 gataacattg attggggaga aattaatcaa cctatcgatg aagaaacttt cttgaagtta
     1861 taccataaag tactagacta tttagataaa aaagatgaac tatacgtatt taaaggctac
     1921 gctggtagcg ataaagatac aatgttaaaa cttacagtca tcaatgaatt agcatggcat
     1981 aatttatttg ctaaaaatat gtttattaga cctgaatcaa aagaagaagc tacaaagatt
     2041 aaacctaact tcactatcgt ttctgcacca cattttaaag cagatccaga agttgatggt
     2101 actaaatctg aaacctttgt cattatttca tttaaacaca aagtcatttt aatcggcggt
     2161 actgaatacg ctggtgaaat gaaaaaaggt atcttctctg taatgaatta tctcttaccg
     2221 atgcaagata ttatgagcat gcattgctca gcaaacgttg gtgaaaaagg cgatgttgca
     2281 ttattctttg gtctatctgg cactggtaaa acaaccttat cggctgaccc acaccgtaaa
     2341 ctaatcggtg atgatgaaca cggatggaat aaaaacgggg tctttaatat cgaaggtggc
     2401 tgctatgcaa aagcaattaa tctttccaaa gaaaaagaac cacagatttt tgacgcaatc
     2461 aaatatggtg caattttaga gaacactgta gttgcagaag atggttcagt ggactttgaa
     2521 gacaatcgtt atacagaaaa cacgcgtgcc gcttatccaa ttaatcacat tgacaatatt
     2581 gtagtaccat caaaagcagc acatccaaat acaattattt tcttaactgc ggatgcattt
     2641 ggtgttattc caccgatttc aaagttaaat aaagaccaag caatgtatca tttcttgagt
     2701 ggtttcactt ctaaattagc tggtacagag cgtggtgtga cagaacctga accatcattc
     2761 tcaacatgtt tcggagcacc gttcttcccg ttacacccta ctgtttacgc tgatttatta
     2821 ggtgaactta tcgatttaca tgatgttgat gtttatcttg ttaatactgg atggactggc
     2881 ggaaaatatg gtgtaggacg tagaatcagc ttacattaca cacgtcaaat ggtaaaccaa
     2941 gcgatttctg gcaaattgaa aaatgcagaa tatacaaaag atagtacgtt tggtttaagc
     3001 attcctgtag aaattgaaga tgtaccgaaa acaattttaa atccaattaa tgcttggagc
     3061 gacaaagaga aatataaagc acaagcagaa gatttaattc aacgttttga aaagaacttc
     3121 gaaaaatttg gtgaaaaagt tgaacatatt gctgaaaaag gtagcttcaa caaataaatt
     3181 tgaatactaa ttaaagagcc aaccgttgtt atagtctaac aatggttggc tcctcttatt
     3241 ttatgtgcta aaaatttata ggcaatttta ttacaacaat gcacatttaa ggtgaccttc
     3301 atgccaaact cgcatcactc atttaatgga agcagcacgt cttcacataa agtaccggtt
     3361 ccttacgcta tgcatgttgc cccactttgc ataaagtact tatcgttaac tcaacgcaag
     3421 ttgttcctat tcacaaaaag cacatacagt tatctgcatg gagcacattt tcattgaaag
     3481 cgcatacccc tcattcaacg tatatcacac atcttcaaag cttgatagtt cccatgcgca
     3541 caccacgttt catactagct atgcaactca acttgtttca taaactcttt aatataagtc
     3601 aatgtttcaa ccatcgctgg tggtcttggc acatgtcctt ctgccatttg ataaaatgtt
     3661 tcatgcgtgg caccttttaa ctctagttgg tccgctaaat aatacgcatg atgaatacca
     3721 acttgctggt ctttccctcc atgtacaatt aatattggcg gactgttttc attaatgttt
     3781 ggaatcgctt ggcgtgcctc atatgccgct cgatcttttt tcggatgacc aatcattctt
     3841 cgtagcatgc ctcttaaatc gacacgttct tcatacatta aatcaatatc tgagacacca
     3901 ccccagattg tataacttgt tactggtaag tcttgaaatg tcaacaatcc ttgtaaacca
     3961 cctcgcgaaa aaccaaccat gtggataaat gcatgtggat atttatcatg tagcaacctt
     4021 aataattgcg tcacatcatt taaatcgcca cggtaaaatt cgtctttacc ttcactccca
     4081 ttgttacctc ggtagtatgg cccaatcact aaagtttgac tatctgaaaa ttgcattaat
     4141 ctacctgcgc gcacacgtcc tacttgacct ttgccacctc gcaaataaac tacaatgcga
     4201 tttacttcat gatgtggtgt catcattaaa gcttttactt gtaagtcatc tgacaaatat
     4261 gtaatttctt cgaattgatg cgtaaaagat tcaattggca ttcgtttacg tttgataaaa
     4321 tccaagtgat tgcaccctct ctacgcattt taaaatggta ctatcttgca gtaagaaact
     4381 ccgttgtgcg agttcaatat cattgataca gttaaacaac actggccctg ctgtttctaa
     4441 ataatcgttc ttgcttacca atgattcaac ttcgataaaa tatacatctt ttacaaaatc
     4501 agtttgatca tgtgtttcaa tggtatattg tgctatgtaa tgaatatttt taactttggc
     4561 gcctgtttct tcatataatt cacgggtaac tgcttcagca ctactttccc cgcgttccct
     4621 tttaccacca ggaaattcaa tcccccgtaa attatgtttg gtaaaaagca attgattttt
     4681 aaacgttgga atagctagca catgattgcc atctgctatc tcattatcct ttttaaatgt
     4741 caaattaact tgacgattat ctttatccct aaacttcacg cgcatcacat ccctacattg
     4801 tatgttaata taatagttaa ttactatcgt tggaggcatt aattatgaaa aagatattct
     4861 tggcgatgat tcatttttat caacgtttca tttcgccact cactccacca acttgtcgtt
     4921 tttatccaac atgttcagag tacactagag aagcgattca ataccacggt gctttcaaag
     4981 gcctttattt aggcatccgt cgtattttaa aatgtcatcc gcttcataaa ggtggctttg
     5041 accctgttcc gttaaaaaaa gacaagtcag caagcaagca ttcacataaa cataaccatt
     5101 aatatggttg taattgagtt atatccacta aaggggggcg aaattcgagt cgcccctctt
     5161 ttaatatgcc tgatttagta accacatctt gatcaaaata ataacctgct ggtgtaacat
     5221 ctcctggata atcaccttta cgagcaagca tcgctgtaaa ataacggctt aaaccatatt
     5281 cgtacatgcc gccaataacc acttttacac catgactttt caaagtatca attgccgttt
     5341 gcactttatc aatgccacct agacgaaatg gttttaatac aacaactttc acattgtata
     5401 attctatcaa attaattatg tccaacaacg atgttgcctt ttcatcaagg gctattggag
     5461 gtattgttcc atccactact tcatcaagca tggagatatc tttaaatggc tcttcgatat
     5521 aaagaacctg ttcacgcgct aatagctgta actgtgtgaa atcttgacga tccaatgact
     5581 catttgcatc tataaccaat tgaaagtgaa agtctaattc ccgtaacact ctaatttgat
     5641 gcatgatttg aggcgtccat tttaatttaa ttctggtcgg ctttgttgct ttcaatgact
     5701 ctagttgttt atttgataag ccgctcgctg tcgctccata tgctactgaa aatgaaggca
     5761 gtacatgaaa catttgatac aatgccatga caatagttgc ccttgcagca ggcgtatttt
     5821 ccaatgaatc tactaatttt agtgctgctt catacgtttc aaatgattta tttctattat
     5881 cttcgaacca ttgctcaatt acatgtttca ctgaagcaat tgtttcatga tcataccaat
     5941 ctgtttgaaa agcgttacat tccccgaaat atgcatttcc tttgtcatca atcaattcga
     6001 taaacaaaca atcacgatgc gttaaagtga ctttcggtgt tacaatttgt gacttaaatg
     6061 gctcactata tttataaaaa tgcaaagctg tcaacttcat caaatcatcc tctatacaac
     6121 ttatttctct gtaatttacc cgttgatgta taaggtaaag tatcaacctt ttcaaagtgt
     6181 ttcggtactt tatatttcgc taaatgtttt gataaatatg caatcaattg tgcctttgaa
     6241 atgtcacttt cactgacaaa atataattta ggcacttggc cccaagtatc atcaggatgc
     6301 cctacacata ctgcgtcact gatacctgga aattgcttcg ctaccgtttc aatttgatat
     6361 ggataaatat tttcaccgcc actaataatt aaatctttac gtcggtcata aatcataaca
     6421 taaccttcat gatctatttc agcaatgtca cctgtattaa aataaccatt ttcaaacgta
     6481 cccgttaaat ctgttggata caaatatcca ttcatcacat tggcaccttt aatcattaat
     6541 tctccatgac cttctttatt aggattttta attttaacgt caacattggc acttggcatc
     6601 cctacagtgt caggacgtgc atgcaacatt tccggtgttg ctgttaaaaa ttgcgaacat
     6661 gtctcagtca taccaaatga attataaatt ggcaggttat attgtaatgc cgtctctatc
     6721 atagtggcag ataatttagc accgccgagt aatatttttt gtaaattata aggttcatgt
     6781 aaaccttgtt gcataagcca atttaaagtt tgtggcacaa gcgaaatgtg cgtgattcgt
     6841 tcatttttaa tcatcgttaa aatttgttcg gcattgaatt tatcaacaat gcgcacagta
     6901 aaaccttcaa taacagctct taaaagtaca ctgagacccg aaatatgata aatcggcaag
     6961 acagatagcc aattagtgtc acgatcaaat cccaagctct ctttacatcc gattgcactg
     7021 gcataatgat tacgaaacgt ttgtggcacc gctttttgag ggcccgttgt ccctgatgta
     7081 aacataattg atgcaatgtc atctaaatta aatgatgtat ttaatatgtt ggacggcgac
     7141 tctttcggca ccacagtttc attcgatgta tcatattgga tacccattgt gttgtccaac
     7201 aaaccgttcg ttgtaatatc ccttccagcg aattcaatat catccagcga tacaatttga
     7261 aaccctcgca attccagtgg caaggtacaa aaaatcaatt gtacatcgat tgacctcatc
     7321 tgattcgtca tctcattagg tgtcaacctt gtattaatca tcgcaatttc aatatttgcc
     7381 aaccaacatg catgtattaa aatgatcgat tgaatcgaat tatctatgta tagcccgaca
     7441 cgagattgtt gataagcctt gagtctttta gccaatagac tcgcttcaca gtataaattt
     7501 tgataagtat aagattcttg accgtctgtt atcgcaatat gatgtccatt ttgttgtgct
     7561 tgtttatata accaaaagtc catgcgttat tcctccaaaa tcatttacat tataattata
     7621 acgattttat gacattctag cagtggttat gtttaaaaat ataaaaaagt agacgaattg
     7681 attcattgat atgattgtta taatgctcaa tacatatcgt tatatcattc gtctactatt
     7741 atcagttatt tttatttaat tttagtgtca ttctgtcatt ttgatgtggt gatttaccca
     7801 ttgttgccac atcatctgca atgtcaattg gtatacgatt catgtcttgt aatgcactta
     7861 aatggaatac ttcatcatct aaattttcaa tgagatatac ataatatgtt accttgtcct
     7921 ttttatattt taacgttttc caaaagtccg gcttgcaatt caatacatta tccggaatat
     7981 attcaataaa taagtaacgt ttgctgccta ctttgtctat gaaatatttt gcagtgcctt
     8041 tttctatacc tcttatatgt gcatagtctg ctgaaaagta aatactacct attgtttcat
     8101 tatgttgttg tatttcaaat cgttggccta ctattttatt atttgtgcca caactactta
     8161 aaaaaatcag tagatatagc attaaacata ttttcatccc cttgaatttt aaaaactttt
     8221 tcaaaagcaa cacctctaaa aataaataca atatattata gcaaatagaa attattattt
     8281 tgttaaatta tgttgtcatg ttcgtgtcat tttgagtcgt aatacaactt tacaattatt
     8341 aaattaacac ttctacaatc aaaaaatatt tttaaagttt tgaaaaaaat taaagtattt
     8401 ccactactaa tttggtaaaa tatgattatg tgatatagtg aaagcgtttt acacttaata
     8461 actccctctt aaatgcatcc aggttttatg tagtaaatca tgaaaataac atataaatat
     8521 agaggagatt tacctttgaa tacagagaac aacaagaatc aaaaccaatc tgttaaaaat
     8581 tctgaaagac gcggcatgtt aaaaggatgc ggcggttgcc ttatttcttt tattttatta
     8641 ataatcttat tatcagcctg ttcaatgatg tttagtaata atgacaattc cactagtaat
     8701 caatcatcaa aaacgcaatt aactcaaaaa gatgaaaata aaaatgaaga taagcctgag
     8761 gaaaaatcag aaacagctac agatgaggat ttacaatcaa ccgaagaagt acccgcaaat
     8821 gaaaatactg aaaataatca acatgaaatt gatgaaataa caacaaaaga tcaatcagat
     8881 gatgaaatta acacaccaaa cgttgcagaa gataaatcac aagacgactt gaaagatgat
     8941 ttaaaagaaa agcaacaatc aagtaaccat catcaatcca cgcaacctaa gacctcacca
     9001 tcaactgaaa caaacaggca acaatcattt gctaattgta agcaacttag acaagtttat
     9061 ccgaatggtg tcactgccga tcatccagca tatcgtccac atttagatag agataaagat
     9121 aatcgtgcat gtgaacctga taaaaattaa acaacaagcg aattgaattc aaattgtatt
     9181 tagctttatg cactaatcac atagtaaata atgagggaga ttttttaggc atgagcaatc
     9241 aattcaaaag tgaagaagaa cgaagacaat gggaacaatt ccaagctttc caaaatcaac
     9301 aaaaccaaca gcaccagcaa tacggacaaa agaaatctaa aaaaggatgg ttctggggtt
     9361 gtggtggttg tctagtatta tttattttaa ttatcatcgg tatttcagct tgtacagctg
     9421 gtgtcactgg taaccttggt ggaaatagtt ctaaagaagc gaacaaaacc cataaaatcg
     9481 gtgaaactgt taaaaatggc gaccttgaag tcactgtaaa ttcagtggaa actatgaaat
     9541 ctgtaggacc gtctcttgca ccaacaaacg ctaaaggtat atttgtcgtt gctgatgtga
     9601 cgattaaaaa caaaggtaaa gaagcgttaa caattgatag ttcaatgttt aagttaaaat
     9661 ccggtgataa aacatttgaa gcagataata caggttcaat gtctgctaat caaagtgaca
     9721 atggtagtat agaaaattca tttttcttac agcgtataaa tcctgatagc actgcacaag
     9781 gtaaaattgt tttcgatgtg tcagaaaaca tagccaacgc aaaagataaa aaattagaag
     9841 ttatttctag tttatttagc gtcaagaaga ttacatttga tttatccgat gctaaaaaaa
     9901 catcaaaagc taaaaaagac aagcaagata cagaagtagc tgttgcgagt tcaaatagcg
     9961 ataatgtaaa ttatgaagct tcggctacta cacctgctac aacttctagt gcggatactg
    10021 attctgaaga tagcgaaaag tctagtaaag atgaggataa acagaatgcg tctaaaagtg
    10081 ataaatctag tgtagaaaaa agtgaatcta atgaggaaac tgctactgta gagcccatgc
    10141 cccagagcaa acctaccact agtgaagcac cacctagtca aaataatcac aacgaaaata
    10201 acatgtatga tgcttcaaca gaataaattt tcagggtagc tcagctaccc ttctttttcg
    10261 gtaaaaataa atatacatag caatataagg agattaaaaa atgaaattca aagctatcgt
    10321 tgcaatcaca ttatcatttt cactattaac tgcctgcggt gctaatcaac ataaagaaaa
    10381 taatagtaaa tcaaatgaca ctactaaaaa gacgcaagaa actgacaaca ctacacagtc
    10441 aaatacagaa aagcaaatga caccacaaga agccgaagat atcgttcgaa acgattacaa
    10501 agcaagaggt gctaacgaaa atcaaacttt aaattataaa acaaatcttg aacggagtaa
    10561 tgaacatgaa tattatgttg aacatctagt ccgcgatgca gttggcacac ctttaaaacg
    10621 ttgcgctatt gttaatcgac acaatggtac gattattaat atttttgatg atatgtcaga
    10681 aaaagataaa gaagaatttg aagcatttaa aaagagaagc cctaaataca acccaggtat
    10741 gagtgatcat gataaaacag atggtgagtc agaagacatt caacatcatg acaatgataa
    10801 taacaaagcc attcaaaatg atacaccaga tcaaaaagcc gttgataaaa acgataaaaa
    10861 tgctgttaat aaagaagaaa aacatgataa ccatgcaaaa aattcagcag aaactaaagt
    10921 taaataaggg tatactttga ttaattgtaa tttttaggag aaaaactatg ataacatttg
    10981 agaatataca acaacttgaa aaatacacct taatgactat gcatggtctt tttaatcaac
    11041 ttaaactcgg tattatttca atcgacaatg cagagcatac cctctttaca ccttatatga
    11101 tggaatcact ctcttcccta ggtgtgaaac cagacattat cgatttaatt cacaaaggga
    11161 cagagttaga agactttgca gcatttaatt tatcaattga agatacagtt acagtctgtt
    11221 tacaaagaac tgaagaacta ttaaatcaat acaaaaatgt ggaattcaat gacaaaatat
    11281 taatcaattg gcgtattata caagagtaat agacatataa aagtcgaatg taactacgtg
    11341 agtactgatt ttattctttg taaattacaa gcatttcata tcataaagtt tgaaaagagg
    11401 tatattgaag tggagaaaaa cgaatataca gctaaatata atgaatatag tcaattatta
    11461 gacgctactt actcgcaagc tgtagcatac cttttaaata aatatggcgc tgtaaccgat
    11521 gattattata aggaaaaatc atacacgcga tttttaaatg gagaaatcaa aagtatttca
    11581 aaaggaaaat acactagagc tagcgaagga ttatattgtc atcacataag cgaagacaaa
    11641 ttccaaaact tatctgacct aagattcatt tccgaattta agtactcata caacgttcaa
    11701 aagaaagaaa acttagtgta ctgtgatcta atcgagcatt taattttaca tgcaattatt
    11761 acaaaagaat cctatggcca atttggtgtg gctggattat gtcaaatgat caaaccaaca
    11821 gtcattgatt ggtacattag cgaatatact ccaaaaccag catggatgca agccaccaaa
    11881 gcacgtgcct atttgcctag aatattagta gagaaactac tcattaaaat tgacgatatg
    11941 ttagaaggaa tagaaatata tgatttcctt gagtctagat aaatgattaa tggagattta
    12001 ttttttgctg ttgagatttt gttatagatg tttaaacctg taattaaata tattttataa
    12061 aatagaccac gcatacctat ctataaacgg acaatgttta taaatgagtt tgcatggtct
    12121 tgaattgtat taaatttctt ttggttttaa tagttcactt agtggtatgt caacattttt
    12181 tagacagttt tcatgtgtat atttttaaat gccactggcg tcaaatactg taatgaagaa
    12241 tgtggtctaa aattgttgta ccaatttaca taatcaaata attctgtttc taactgttct
    12301 aagttttcaa attgcatttg ttttacaaat tctgttttca ttgctttcat cgttgcttcc
    12361 gcaactgcgt tatcataagg acaacctttg gtacttaatg aacgttttat tttaaatgtt
    12421 tctaggactt catctatcaa atgattatca aattctttgt ctctatcagt gtggaatagt
    12481 ttgatttgtt caagattatg atttattctg ctgattgctt ttgatactaa attggcgtcc
    12541 ttatttttac ctgcactgta accaacaatt tctctattaa atagatctat aaataaacat
    12601 atgtaatgcc atgttcctcc gacttttaca tatgtcaaat cacttactaa tgtctccatt
    12661 ggttgttctc tattaaaagc gcgattcaaa tgatttttaa ttcgtttttc attagtttct
    12721 atttgatgat ttttgtattt agctttcata taaacagaaa ctagattttt tcataatcga
    12781 cctatctttt gtccagatac agtgagaccc ttgtcattta aatgattttt aattcgtctt
    12841 gtactaaaga cttttctatt agaattaaaa atatttatgg cggcacgttc tacgtttgaa
    12901 tcatctttag tgattttatt atcttttctt tttatagaat cataataggt acttcttggt
    12961 atttttagga ctttacacat tgctggtact gaatattgat gtgcattctt ttgaatgact
    13021 tctatttttg ccccataatc agctcttttt cttcatctgt taagttatct tggtgattga
    13081 atgtaccctt gttttgatgt tactttatcc attttcctaa cgtcaaaggt gttaaatcat
    13141 actcgcgtat aatttcattt cttggcttac cattttcata taatctaacc atttataact
    13201 taaactctga actaaatgtt cttctttctt aaattaacaa aatctactct catagaattt
    13261 gtccaattag atgatataat agtagagtcg cctatctctc aggcgtcaat ttagacgcag
    13321 agaggaggtg tataaggtga tgcttatttt cgttcacatc atagcaccag tcatcagtgg
    13381 ctgtgccatt gcgttttttt cttattggct aagtagacgc aatacaaaat aggtgacata
    13441 tagccgcacc aataaaaatc ccctcactac cgcaaatagt gaggggattg gtgtataagt
    13501 aaatacttat tttcgttatc tcaattatac tgctaatctt tctctttgta aaatatgcaa
    13561 ggttttaaag agaaacatca agaactaaaa aaggctctat gccaaattgg cctgatgagt
    13621 tcaatatcgg aagttaggca actaaacatt gcttagcttc ctttttactt tttggagcgt
    13681 aaagttttga acataataat attcgatttc gcaaatgatt ataacttcct taaccaaaag
    13741 atgtacgttt aattaatttt attttgttgt ttatcccttc taaaggacca tttgattatc
    13801 cttcgtttta aagttaatgc cttctttcca gtcaaactgt gcttgatgac tggcttttgt
    13861 ctcgaatctc gttttgcctt taggcgacaa ccgttgataa cctttcataa aataacgatt
    13921 aaattcatca tgtttaagga tataagctct aaatgtagaa tatgcacagg ttaatccatg
    13981 tttatctttc aaatattgcc aaagaataag cttatagtag aacttttgtt cactagagtc
    14041 tgaaagtaat ttttcgatga taggataata cttgtcaata atagactgac gatttctctt
    14101 tttggttggc tcaaagccat ttaaatattt atcaactgtt cttctataaa cacccatgtg
    14161 tctcgctatt tcacttttgt ttattttcat gtttaagttc tccat
//
LOCUS       DAHHSC010000052        13765 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.052, whole genome shotgun sequence.
ACCESSION   DAHHSC010000052 DAHHSC010000000
VERSION     DAHHSC010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 13765)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13765)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13765
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.052"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            130..423
                     /gene="esxA"
                     /locus_tag="NBI57_001174"
     CDS             130..423
                     /gene="esxA"
                     /locus_tag="NBI57_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WXG100 family type VII secretion effector EsxA"
                     /protein_id="HCD1822131.1"
                     /translation="MAMIKMSPEEIRAKSQSYGQGSDQIRQILSDLTRAQGEIAANWE
                     GQAFSRFEEQFQQLSPKVEKFAQLLEEIKQQLNSTADAVQEQDQQLSNNFGLQ"
     gene            506..3535
                     /gene="esaA"
                     /locus_tag="NBI57_001175"
     CDS             506..3535
                     /gene="esaA"
                     /locus_tag="NBI57_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VII secretion protein EsaA"
                     /protein_id="HCD1822132.1"
                     /translation="MKKKNWIYALIVTLIIIIAIVSMIFFVQTKYGDQSEKGSQSVSN
                     KNNKIHIAIVNEDQPTTYNGKKVELGQAFIKRLANEKNYKFETVTRNVAESGLKNGGY
                     QVMIVIPENFSKLAMQLDAKTPSKISLQYKTAVGQKEEVAKNTEKVVSNVLNDFNKNL
                     VEIYLTSIIDNLHNAQKNVGAIMTREHGVNSKFSNYLLNPINDFPELFTDTLVNSISA
                     NKDITKWFQTYNKSLLSANSDTFRVNTDYNVSTLIEKQNSLFDEHNTAMDKMLQDYKS
                     QKDSVELDNYINALKQMDSQIDQQSSMQDTGKEEYKQTVKENLDKLREIIQSQESPFS
                     KGMIEDYRKQLTESLQDELANNKDLQDALNSIKMNNAQFAENLEKQLHDDIVKEPDTD
                     TTFIYNMSKQDFIAAGLNEGEANKYEAIVKEAKRYKNEYNLKKPLAEHINLTDYDNQV
                     AQDTSSLINDGVKVQRTETIKSNDINQLTVATDPHFNFEGDIKINGKKYDIKDQSVQL
                     DTSNKEYKVEVNGVAKLKKDAEKDFLKDKTMHLQLLFGQANRQDEPNDKKATSVVDVT
                     LNHNLDGRLSKDALSQQLSALSRFDAHYKMYTDTKGREDKPFDNKRLIDMMVDQVIND
                     MESFKDDKVAVLHQIDSMEENSDKLIDDILNNKKNTTKNKEDISKLIDQLENVKKTFA
                     EEPQEPKIDKGKNDEFNTMSSNLDKEISRISEKSTQLLSDTQESKTIADSVSGQLNQL
                     DNNVNKLHATGRALGVRANDLNRQMAKNDKDNELFAKEFKKVLQNSKDGDRQNQALKA
                     FMSNPVQKKNLENVLANNGNTEVISPTLFVLLMYLLSMITAYIFYSYERAKGQMNFIK
                     DDYSSKNHLWNNVITSGVIGTTGLVEGLIVGLIAMNKFHVLAGYRAKFILMVILTMMV
                     FVLINTYLLRQVKSIGMFLMIAALGLYFVAMNNLKAAGQGVTNKISPLSYIDNMFFNY
                     LNAEHPIGLALVILTVLVIIGFVLNMFIKHFKKERLI"
     gene            3535..3993
                     /gene="essA"
                     /locus_tag="NBI57_001176"
     CDS             3535..3993
                     /gene="essA"
                     /locus_tag="NBI57_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VII secretion protein EssA"
                     /protein_id="HCD1822133.1"
                     /translation="MLMNSVIALTFLTASSNNGGLNIDVQQEEEKRINNDLNQYDTTL
                     FNKDSKEVNDAIAKQKKERQQQIKNDMFQNQASHSTRLNETKKVLFSKSNLEKTSESD
                     KSPYIQNKQEKKIFPYILMSVGAFLTLGFVIFSIHKGRRTKNESARKSNI"
     gene            3965..4207
                     /gene="esaB"
                     /locus_tag="NBI57_001177"
     CDS             3965..4207
                     /gene="esaB"
                     /locus_tag="NBI57_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001071606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VII secretion protein EsaB"
                     /protein_id="HCD1822134.1"
                     /translation="MNQHVKVTFDFTNYNYGTYDLAVPAYLPIKNLIALVLDSLDISI
                     FDVNTQIKVMTKGQLLVENDRLIDYQIADGDILKLL"
     gene            4220..5554
                     /gene="essB"
                     /locus_tag="NBI57_001178"
     CDS             4220..5554
                     /gene="essB"
                     /locus_tag="NBI57_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VII secretion protein EssB"
                     /protein_id="HCD1822135.1"
                     /translation="MVKNHNPKNEMQDMLTPLDAEEAAKTKLRLDMREIPKSSIKPEH
                     FHLMYLLEQHSPYFIDAELTELRDSFQIHYDINDNHTPFDNIKSFTKNEKLRYLLNIK
                     NLEEVNRTRYTFVLAPDELFFTRDGLPIAKTRGLQNVVDPLPVSEAEFLTRYKALVIC
                     AFNEKQSFDALVEGNLELHKGTPFETKVIEAATLDLLTAFLDEQYQKQEQDYSQNYAY
                     VRKVGHTVFKWVAIGMTTLSVLLIAFLAFLYFSVMKHNERIEKGYQAFVKDDYTQVLN
                     TYDDLDGKKLDKEALYIYAKSYIQTNKQGLEKDKKENLLNNVTPNSNKDYLLYWMELG
                     QGHLDEAINIATYLDDNDITKLALINKLNEIKNNGDLSNDKRSEETKKYNDKLQDILD
                     KEKQVKDEKAKSEEEKAKAKDEKLKQQEENEKKQKEQAQKDKEKRQEAERKK"
     gene            5576..10015
                     /gene="essC"
                     /locus_tag="NBI57_001179"
     CDS             5576..10015
                     /gene="essC"
                     /locus_tag="NBI57_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VII secretion protein EssC"
                     /protein_id="HCD1822136.1"
                     /translation="MHKLIIKYNKQLKMLNLRDGKTYTISEDERADITLKSLGEVIHL
                     EQNNQGTWQANHTSINKVLVRKGDLDDITLQLYTEADYASFAYPSIQDTMTIGPNAYD
                     DMVIQSLMNAIIIKDFQSIQESQYVRIVHDKNTDVYINYELQEQLTNKAYIGDHIYVE
                     GIWLEVQADGLNVLSQNTVASSLIRLTQEMPHAQADDYNTYHRSPRIIHREPTDDIKI
                     ERPPQPIQKNNTVIWRSIIPPLVMIALTVVIFLVRPIGIYILMMIGMSTVTIVFGITT
                     YFSEKKKYNKDVEKREKDYKDYLDNKSKEINKAIKAQRFSLNYHYPTVAEIKDIVETK
                     APRIYEKTSHHHDFLHYKLGIANVEKSFKLDYQEEEFNQRRDELFDDAKELYEFYTDV
                     EQAPLINDLNHGPIAYIGARHLILEELEKMLIQLSTFHSYHDLEFLFVTREDEVETLK
                     WARWLPHMTLRGQNIRGFVYNQRTRDQILTSIYSMIKERIQAVRERSRSNEQIIFTPQ
                     LVFVITDMSLIIDHVILEYVNQDLSEYGISLIFVEDVIESLPEHVDTIIDIKSRTEGE
                     LITKEKELVQLKFTPENIDNVDKEYIARRLANLIHVEHLKNEIPDSITFLEMYNVKEV
                     DQLDVVNRWRQNETYKTMAVPLGVRGKDDILSLNLHEKAHGPHGLVAGTTGSGKSEII
                     QSYILSLAINFHPHEVAFLLIDYKGGGMANLFKDLVHLVGTITNLDGDEAMRALTSIK
                     AELRKRQRLFGEHDVNHINQYHKLFKEGVATEPMPHLFIISDEFAELKSEQPDFMKEL
                     VSTARIGRSLGIHLILATQKPSGVVDDQIWSNSKFKLALKVQYRQDSNEILKTPDAAD
                     ITLPGRAYLQVGNNEIYELFQSAWSGATYDIEGDKLEVEDKTIYMINDYGQLQAINKD
                     LSGLEDEETKENQTELEAVIDHIESITTRLEIEEVKRPWLPPLPENVYQEDLVETDFR
                     KLWSDDAKEVELTLGLKDVPEEQYQGPMVLQLKKAGHIALIGSPGYGRTTFLHNIIFD
                     VARHHRPDQAHMYLFDFGTNGLMPVTDIPHVADYFTVDQEDKIAKAIRIFNDEIDRRK
                     KILSQYRVTSISEYRKLTGETIPYVFILIDNFDAVKDSPFQEVFENMMIKMTREGLAL
                     DMQVTLTASRANAMKTPMYINMKTRIAMFLYDKSEVSNVVGQQKFAVKDVVGRALLSS
                     DDNVSFHIGQPFKHDETKSYNDQINDEVSAMTEFYKGETPNDIPMMPDEIKYEDYRES
                     LSLPDIVANGALPIGLDYEGVTLQKIKLTEPAMISSENPREIAHIAEIMMKEIDILNE
                     KYAICIADSSGEFKAYRHQVANFAEEREDIKAIHQLMIEDLKQREMDGPFEKDSLYII
                     NDFKTYIDCTYIPEDDVKKLITKGPELGLNILFVGIHKELIDAYDKQIDVARKMINQF
                     SIGIRISDQQFFKFRFIQREPVIKENEAYMVANQAYQKIRWFK"
     gene            10045..10437
                     /gene="esaC"
                     /locus_tag="NBI57_001180"
     CDS             10045..10437
                     /gene="esaC"
                     /locus_tag="NBI57_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VII secretion substrate EsaC"
                     /protein_id="HCD1822137.1"
                     /translation="MNFNDIETMVKSKFKDIKKHAEEIAHEIEVRSGYLRKAEQYKRL
                     EFNLSIALDDVESTAKDVQTAKSSANKDSVSVKGKAPNTLYIEKRNLMKQKLEMLGED
                     IDKNKESLQKAKGIAGEKASEYFNKAMN"
     gene            10453..10767
                     /gene="esxB"
                     /locus_tag="NBI57_001181"
     CDS             10453..10767
                     /gene="esxB"
                     /locus_tag="NBI57_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WXG100 family type VII secretion effector EsxB"
                     /protein_id="HCD1822138.1"
                     /translation="MGGYKGIKADGGKVNQAKQLAAKIAKDIEACQKQTQQLAEYIEG
                     SDWEGQFANKVKDVLLIMAKFQEELVQPMADHQKAIDNLSQNLAKYDTLSIKQGLDRV
                     NP"
     gene            10767..11441
                     /locus_tag="NBI57_001182"
     CDS             10767..11441
                     /locus_tag="NBI57_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5081 family protein"
                     /protein_id="HCD1822139.1"
                     /translation="MKDVKRIDYFSYEELTILGGSKLPLVNFELFDPSNFEEAKVALI
                     EKELVTENDKLTDAGFKVATLVREYISAIVNIRINDMYFAPFSYEKDEYILLSRFKNN
                     GFQIRIINKDIAWWSIVQSYPLLMRQEKSNDWDFKQIDDEILENLNNESIDTIGRVLE
                     IEIYNHQGDPQQSLYNIYEQNDLLLIRYPLKDKVLNVHIGVINTFIRELFGFDTDENH
                     INKAEE"
     gene            11441..11758
                     /locus_tag="NBI57_001183"
     CDS             11441..11758
                     /locus_tag="NBI57_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1822140.1"
                     /translation="MTLSGKISVKAETIAHVVKELESISQKYDEIAQNFGKIAQLNYY
                     SSEKAAHSMENGYSSAATVISGLKGPLSTLGGGVMNSAQKFFEADEHWGTEFAKLYYN
                     IEG"
     gene            11768..13612
                     /locus_tag="NBI57_001184"
     CDS             11768..13612
                     /locus_tag="NBI57_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease YeeF family protein"
                     /protein_id="HCD1822141.1"
                     /translation="MTKDIEYLTADYDNEKSSIQSVIDAIEGQDFLDVDTTMDDAVSD
                     VSSLDEDGAISLTSSVVGPQGSKLMGYYQNELYDYASQLDSKMKEIIDTPFIEDIDKA
                     FKGITNVKLENILIKNGGGHGRDTYGASGKIAKGDAKKSDSDVYSIDEILKSDQEFVK
                     VIDQHYKEMKKEDKKLSKSDFEKMMTQGASCDYMTVAEAEEIEEQKKKEEAIEIAALA
                     GMVVLSCINPVAGAVAIGAYSAYSAANAATGKNIVTGRKLSKEERIMEGLSLIPLPGM
                     GFLKGAGKSLMKLGFKGGEKFAVKTGLQKTMQQAVSRISPKMGMMKNSVLNQSRNFAQ
                     NTHVGQMLSNMRGQATHTVQQSRNWIGQQAQNVKRIVNNGLDKEIAHPFKQQLAPAGM
                     GGIKFAETTTLRNMGQNMKRAVTPQNHVTHGPKDSMVRSEGKHSVSSHEINLSKYVES
                     PNYTKVKYGEQYARLRPKKLKANIEYTTPTGHIYRTDHKGRIKEVYVDNLSLKDGGRN
                     NHAQRTVGGEDRLPDDDGGHLIARMFGGSKDIDNLVAQSKYINRSFKENGDWYKLEKL
                     WEKAIKSGKSIENIKIEVKYKGNSQRPTIFKVEYEINGKRNIRRILNK"
     gene            13623..>13765
                     /locus_tag="NBI57_001185"
     CDS             13623..>13765
                     /locus_tag="NBI57_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF600 family protein"
                     /protein_id="HCD1822142.1"
                     /translation="MTFEEKLSKIYNEIANEISNMIPVEWEKVYTMAYIDDGGGEVFF
                     NYT"
BASE COUNT         5406 a         1898 c         2598 g         3863 t
ORIGIN      
        1 taaaaatact taaaaaacta tatacatata ctaataattt atatattatt tgagtaagga
       61 gcactttttc aaaaaatagt gtccctaaaa agttttgata aacttaaaat attcaggagg
      121 tttctagtta tggcaatgat taagatgagt ccagaggaaa tcagagcaaa atcgcaatct
      181 tacgggcaag gttcagacca aatccgtcaa attttatctg atttaacacg tgcacaaggt
      241 gaaattgcag cgaactggga aggtcaagct ttcagccgtt tcgaagagca attccaacaa
      301 cttagtccta aagtagaaaa atttgcacaa ttattagaag aaattaaaca acaattgaat
      361 agcactgctg atgccgttca agaacaagac caacaacttt ctaataattt cggtttgcaa
      421 taagcattct gaaattggca aagtcacatt ttctaatgtg gctttgctta tcatttttta
      481 agaaaacaac tgaaaggaaa taagcatgaa aaagaaaaat tggatttatg cattaattgt
      541 cactttaatt attataattg ccatagttag tatgatattt tttgttcaaa caaaatatgg
      601 agatcaatca gaaaaaggat cccaaagtgt aagtaataaa aataataaaa tacatatcgc
      661 aattgttaac gaggatcaac caacgacata taatggtaaa aaagttgagc tgggtcaagc
      721 atttattaaa aggttagcaa atgagaaaaa ctataaattt gaaacagtaa caagaaacgt
      781 tgctgagtct ggtttgaaaa atggtggata ccaagtcatg attgttatcc cagaaaactt
      841 ttcaaaattg gcaatgcaat tagacgctaa aacaccatcg aaaatatcgc tacagtataa
      901 aacagctgta ggacaaaaag aagaagtagc taaaaacaca gaaaaagttg taagtaatgt
      961 acttaacgac tttaacaaaa acttagtcga aatttattta acaagcatca ttgataattt
     1021 acataatgca caaaaaaatg ttggcgctat tatgacgcgt gaacatggtg tgaatagtaa
     1081 attctcgaat tacttattaa atccaattaa cgacttcccg gaattattta cagatacgct
     1141 tgtaaattca atttctgcaa acaaagacat tacaaaatgg ttccaaacat acaataaatc
     1201 attattgagt gcgaattcag atacgttcag ggtgaacaca gattataatg tttcgacttt
     1261 aattgaaaaa caaaattcat tatttgacga gcacaataca gcgatggata aaatgttaca
     1321 agattataaa tcgcaaaaag atagtgtgga acttgataac tatatcaatg cattaaaaca
     1381 gatggacagc caaattgatc aacaatcaag tatgcaagat acaggtaaag aagaatataa
     1441 acaaactgtt aaagaaaact tagataaatt aagagaaatc attcaatcac aagagtcacc
     1501 attttcaaaa ggtatgattg aagactaccg taagcaatta acagaatcac tgcaagatga
     1561 gcttgcaaat aacaaagact tacaagatgc gctaaatagc attaaaatga acaatgctca
     1621 attcgctgaa aacttagaaa aacaacttca tgatgatatt gtcaaagaac ctgatacaga
     1681 tacaacattt atctataaca tgtctaaaca agactttata gctgcaggtt taaatgaggg
     1741 tgaagctaat aaatacgaag caattgtcaa agaagcaaaa cgttataaaa acgaatataa
     1801 tttgaaaaaa ccgttagcag aacacattaa tttaacagat tacgataacc aagttgcgca
     1861 agacacaagt agtttgatta atgatggtgt caaagtgcaa cgtactgaaa cgattaaaag
     1921 taatgatatt aatcaattaa ctgttgcaac agatcctcat tttaactttg aaggcgacat
     1981 taaaattaat ggtaaaaaat atgacattaa ggatcaaagt gttcaactcg atacatctaa
     2041 caaggaatat aaagttgaag tcaatggcgt tgctaaattg aaaaaggatg ctgagaaaga
     2101 tttcttaaaa gataaaacaa tgcatttaca attgttattt ggacaagcaa atcgtcaaga
     2161 tgaaccaaat gataagaaag caacgagtgt tgtggatgta acattgaatc ataaccttga
     2221 tggtcgctta tcgaaagatg cattaagcca gcaattgagt gcattatcta ggtttgatgc
     2281 acattataaa atgtacacag atacaaaagg cagagaagat aaaccattcg ataacaaacg
     2341 tttaattgat atgatggttg accaagttat caatgacatg gaaagtttca aagacgataa
     2401 agtagctgtg ttacatcaaa ttgattcaat ggaagaaaac tcagacaaac tgattgatga
     2461 cattttaaat aacaaaaaga atacaacaaa aaataaagaa gatatttcta agctgattga
     2521 tcagttagaa aacgttaaaa agacttttgc tgaagaacca caagaaccaa aaattgacaa
     2581 aggcaaaaat gatgaattca acacaatgtc ttctaactta gataaagaaa ttagcagaat
     2641 ttctgaaaag agcacgcaat tgctatctga tacacaagaa tcaaaaacaa ttgcagattc
     2701 agttagtgga caattaaatc aattagataa taatgtgaat aaactacatg cgacaggtcg
     2761 agcattaggc gtaagagcga atgatttgaa ccgtcaaatg gctaaaaacg ataaagataa
     2821 tgagttattc gctaaagagt ttaaaaaagt attacaaaat tctaaagatg gcgacagaca
     2881 aaaccaagca ttaaaagcat ttatgagtaa tccggttcaa aagaaaaact tagaaaatgt
     2941 tttagctaat aatggtaata cagaggtgat ttcaccgaca ttattcgtat tattgatgta
     3001 tttactatca atgattacag catatatttt ctatagttat gaacgtgcca aaggtcaaat
     3061 gaatttcatt aaagatgatt atagtagtaa aaaccatctt tggaataatg tcattacgtc
     3121 aggtgttatt ggtacaactg gtttggtaga aggattaatt gtcggtttaa ttgcaatgaa
     3181 taagttccat gtattagctg gctatagagc gaaattcatc ttaatggtga ttttaactat
     3241 gatggtcttt gtacttatta atacgtattt actaagacag gtaaaatcta tcggtatgtt
     3301 cttaatgatt gctgcattgg gtctatactt tgtagctatg aataatttga aagcggctgg
     3361 acaaggtgtg actaataaaa tttcaccatt gtcttatatc gataacatgt tcttcaatta
     3421 tttaaatgca gagcatccta taggcttggc actagtaata ttaacagtac ttgtgattat
     3481 tggttttgta ctgaacatgt ttataaaaca ctttaagaaa gagagattaa tctaatgttg
     3541 atgaatagcg tgattgcttt aactttttta acagcatcta gcaataatgg cggacttaat
     3601 attgatgtgc aacaagaaga ggaaaagcga atcaataatg atttaaatca atatgataca
     3661 acgctattta ataaagatag taaagaagtc aacgacgcga ttgctaagca gaaaaaagaa
     3721 cgacaacaac aaataaaaaa tgatatgttt caaaatcaag cgagtcactc gactcgcttg
     3781 aatgaaacta aaaaagtgtt attttccaaa tctaacttag aaaagacttc ggagagtgat
     3841 aaaagcccct atattcaaaa caagcaggag aaaaaaatat tcccgtacat tttgatgtct
     3901 gtaggggctt ttttgacttt aggatttgtc attttttcaa ttcataaagg gagacgaacg
     3961 aaaaatgaat cagcacgtaa aagtaacatt tgattttact aattataatt acggcacata
     4021 tgacttagca gtaccagcat atttaccgat aaaaaattta atagctttag tattggatag
     4081 tttggacatt tcaatatttg atgtcaatac acaaattaaa gtgatgacga aaggtcaatt
     4141 acttgttgaa aatgatcgac tcattgatta tcaaatcgct gatggagata ttttgaagtt
     4201 actataggag gaaaaataga tggttaaaaa tcataaccct aaaaatgaaa tgcaagatat
     4261 gttaacgcct ttagatgctg aagaagcagc taaaacaaaa ttacgcttag atatgagaga
     4321 gattcctaag tcttcaatta aaccagaaca ttttcattta atgtacttat tagaacaaca
     4381 ttctccatat tttatagatg ctgaattaac tgaactacgt gacagtttcc aaatacatta
     4441 tgacattaat gacaatcata caccttttga taatattaaa tcatttacta aaaatgaaaa
     4501 attacgatac ttactcaata tcaaaaattt agaagaagta aatcgtacac gttacacatt
     4561 tgttttggca ccagatgagt tatttttcac aagagatgga ttaccaattg ctaaaacaag
     4621 aggtttacaa aatgttgttg atccattacc ggtgtcagaa gctgaatttt taacaagata
     4681 taaagcactg gttatctgtg cattcaatga gaaacaatca tttgatgctt tagttgaagg
     4741 aaacttagaa ctacataaag gaacgccatt tgaaactaaa gttattgaag cggcaacgtt
     4801 agatttacta acggcatttt tagatgaaca gtatcagaaa caagaacaag attatagtca
     4861 aaattatgca tatgtacgca aagtagggca taccgttttc aaatgggttg ctatcggcat
     4921 gacaacgtta agtgtgttat taattgcatt tttagccttt ttatattttt cagtaatgaa
     4981 gcataatgag cgcattgaaa aaggatacca agcatttgta aaggatgatt atacgcaagt
     5041 actaaatacg tatgatgatt tagatggaaa aaaattagat aaagaggcac tttacattta
     5101 tgccaaaagt tatatccaaa caaataaaca aggtttagaa aaagataaga aagaaaattt
     5161 acttaataac gtgacaccaa attcaaacaa agactactta ttatattgga tggaattagg
     5221 acaaggacat cttgatgaag cgattaatat tgccacttat ttagatgata acgatattac
     5281 aaagttagcg ttgattaata aattaaatga gattaaaaat aacggagatt tatcgaatga
     5341 taaacgttct gaagaaacga aaaagtataa cgataaattg caagatattt tagacaaaga
     5401 aaaacaagtt aaagatgaaa aagcgaaatc tgaagaagag aaagcaaaag cgaaagatga
     5461 gaaattaaag caacaagaag agaacgaaaa gaaacaaaaa gaacaagcac aaaaagataa
     5521 agaaaaacgc caagaagctg aaagaaaaaa atagtatagg actgaggcaa agacaatgca
     5581 taaattgatt ataaaatata acaaacaatt gaagatgctc aatttgcgag atggtaagac
     5641 atatactatt agcgaagacg agcgtgcaga tattacgttg aaatcgttag gcgaagtcat
     5701 tcatttagaa caaaataatc aaggtacttg gcaagcgaat catacttcta ttaataaggt
     5761 gcttgttaga aaaggtgacc ttgatgacat tacattacag ctttatacag aagctgatta
     5821 tgcatcattt gcgtatcctt caattcaaga tacgatgaca attgggccaa atgcgtatga
     5881 tgatatggtt attcaaagct tgatgaatgc catcattatt aaagattttc aatcaataca
     5941 agaatcacaa tacgtacgca ttgtgcacga taaaaataca gatgtgtata ttaactatga
     6001 actacaagag caattaacaa acaaagctta cattggtgat catatttatg ttgaagggat
     6061 atggctcgaa gtacaagctg atggtttaaa tgtattgagt cagaatacag tggcatcgtc
     6121 attaattcgc ttaacacaag agatgccaca tgcacaggca gatgattaca atacgtacca
     6181 tcgttcgcca aggattattc accgtgaacc gaccgatgat attaagattg aaagaccgcc
     6241 acagccaata cagaagaaca atacagtgat atggcgttcc attataccgc cattagtaat
     6301 gattgcttta actgttgtca tctttttagt gagaccaatt ggtatttata ttttaatgat
     6361 gattggtatg agtacagtaa cgatagtatt tggtattaca acgtatttct ctgaaaagaa
     6421 aaagtataac aaagatgttg aaaaacgaga gaaagattac aaagattatt tggataataa
     6481 atctaaagaa attaataaag cgattaaagc acaacgtttt agtttgaatt accattatcc
     6541 aacggttgct gaaattaaag atatcgttga aacgaaagca ccaagaatat atgaaaaaac
     6601 atcgcatcat cacgatttct tacattataa gttaggtatt gcgaatgtag aaaagtcatt
     6661 caaattagat taccaagaag aagaatttaa ccaacgtcgt gatgaactat tcgacgatgc
     6721 taaagaattg tatgaatttt acacagatgt agaacaagca ccattaatca atgatttaaa
     6781 tcatggacca attgcatata ttggtgcacg acatctcatt ttagaagaat tggagaaaat
     6841 gctaatccaa ttgtcaacgt tccatagtta tcatgattta gagtttctat ttgtgacacg
     6901 tgaagatgaa gttgaaacat tgaaatgggc acgttggttg ccacatatga cattgagagg
     6961 tcaaaacatt agagggtttg tttacaatca acgaacacgt gaccaaattt taacgtcaat
     7021 ttatagcatg attaaagaac gtatccaagc tgtgcgtgaa cgcagcagaa gtaatgagca
     7081 aattattttc acaccgcaat tagtgtttgt cattacagat atgtcattaa ttattgatca
     7141 tgtcatttta gaatatgtaa accaagattt atcagaatat ggtatttcat taatctttgt
     7201 tgaagatgtg attgaaagtt tgccagagca tgtagatacc attattgata tcaagtctcg
     7261 tactgaaggc gaactgatta cgaaagaaaa agaattagtt caattgaaat ttacacctga
     7321 aaatattgat aacgttgata aagaatatat cgcgagacgt ttggcgaatt tgatacacgt
     7381 cgaacatttg aaaaatgaaa ttcctgatag tattacattt ttagagatgt ataacgtgaa
     7441 agaagtagat cagcttgatg tggttaatcg atggagacaa aacgaaacat acaaaacgat
     7501 ggcagtacct ttaggtgtaa gaggtaaaga tgatatttta tcattgaact tacatgaaaa
     7561 agcacacggg ccacatggtt tagttgctgg taccactggt tcagggaaat ctgagattat
     7621 ccaatcatac attttatctt tagctattaa ttttcatcca catgaagttg cgttcctatt
     7681 gattgactat aaaggtgggg gtatggcgaa cttatttaaa gatttagtcc atttagttgg
     7741 tacgattaca aacttagatg gcgatgaagc gatgcgtgcc ttaacatcaa tcaaagccga
     7801 attgagaaaa cgtcaacgtt tattcggaga gcatgatgtt aaccatatta atcaatacca
     7861 taagttattt aaagaaggtg ttgcaacaga accaatgcca catttattca ttatttccga
     7921 tgagtttgcc gaattaaaat cagaacaacc tgattttatg aaagaacttg tatcaacggc
     7981 acgtattgga cgttcgttag gtattcattt aatacttgcg acacaaaaac catcgggtgt
     8041 tgttgatgac caaatttggt ctaactctaa atttaaattg gcattaaaag tacaatatag
     8101 acaagacagt aatgaaattt taaaaacacc agatgcagca gacattacat taccaggtcg
     8161 tgcgtattta caagttggta ataatgaaat ttatgaatta ttccaatctg catggagtgg
     8221 tgcaacatat gacatcgaag gcgataaatt agaagttgaa gataagacga tttacatgat
     8281 taatgactat ggtcaacttc aagcaatcaa caaagacttg agtggacttg aagatgaaga
     8341 aacgaaagaa aaccaaaccg agttagaagc ggttatcgat catatcgaat ctattacaac
     8401 acgattagaa attgaagaag ttaagcgtcc atggttacca ccattaccag aaaatgtata
     8461 tcaagaagat ttagtagaaa cagatttcag aaaattatgg tcagatgatg caaaagaagt
     8521 ggaattaaca ttaggactta aagacgtacc agaagaacaa tatcaaggac cgatggtatt
     8581 gcaattgaaa aaagctgggc acatcgcgtt aatcggaagt ccaggatatg gtagaacaac
     8641 gttcttacac aacattattt tcgatgttgc aagacaccat cgtcctgatc aagcacacat
     8701 gtacttgttc gatttcggta ccaatggttt gatgccagtc acagatatac cacatgttgc
     8761 tgattatttt acagtagatc aagaagacaa gattgctaag gcgatacgta tatttaatga
     8821 tgaaatcgat cgtcgtaaga agattttaag tcagtatcgt gttactagta tttctgaata
     8881 tcgaaaatta actggtgaaa caattccgta tgtctttatt cttattgata actttgacgc
     8941 agtaaaagat tcacctttcc aagaagtttt tgaaaatatg atgattaaaa tgacacgtga
     9001 agggctagca ttagacatgc aagtaacctt aactgcttca agagctaacg caatgaaaac
     9061 gccaatgtac attaatatga aaacgcgtat cgccatgttt ttatatgata aatcagaggt
     9121 gtcgaacgta gtagggcaac aaaaatttgc ggttaaagat gtagtgggtc gagcattgtt
     9181 aagtagtgat gacaatgtat cattccatat tggccaacca tttaaacatg atgagactaa
     9241 atcatataat gatcaaatta atgatgaagt atcggcgatg acagaatttt ataaaggtga
     9301 aacaccaaat gatattccta tgatgccaga tgaaattaaa tatgaagatt acagagaatc
     9361 attaagctta ccagatatag ttgccaatgg tgctttacca attggattag attatgaagg
     9421 tgttacacta caaaaaatta aattaactga accagcaatg atttcatcag aaaatccgag
     9481 agaaattgcg catattgccg aaattatgat gaaagaaatt gacatattaa atgaaaaata
     9541 tgcgatttgt atcgcagact caagtggaga gtttaaagct tataggcatc aagtggctaa
     9601 ctttgccgaa gaaagagaag acattaaagc gattcatcaa ctaatgattg aagacttaaa
     9661 acaaagagaa atggacggtc catttgaaaa agattcactt tacattatca atgattttaa
     9721 aacatatatt gattgcacgt atatcccgga agatgatgtt aaaaagctta ttacaaaagg
     9781 accagaactt ggcttgaaca ttttatttgt cggcattcat aaagaattaa tagatgctta
     9841 tgataaacag attgatgttg cacgtaaaat gattaaccaa tttagtatag gtattcgtat
     9901 ttcagaccaa caattcttta aatttagatt tattcaacga gaacctgtta ttaaagaaaa
     9961 cgaagcgtat atggtcgcga atcaagctta tcaaaagatt agatggttta aatagtaatg
    10021 aattaaatag gagggaggta tgttatgaat tttaatgata ttgaaacaat ggtcaagtcg
    10081 aaatttaaag atattaaaaa gcatgctgaa gagattgcgc atgaaattga agttcgttct
    10141 ggatatttaa gaaaagctga acaatataag cgattagaat ttaatttaag tattgcacta
    10201 gatgatgttg aaagcacagc aaaggacgta caaactgcaa aatctagtgc taataaagac
    10261 agtgtatctg ttaaaggaaa agcgcccaat acgttatata tcgaaaaaag aaatttgatg
    10321 aaacaaaagc ttgaaatgtt gggtgaagat atcgataaaa ataaagaatc cctccaaaaa
    10381 gctaagggaa ttgctggcga aaaggcaagt gaatatttta ataaagcaat gaattaatat
    10441 tgaggtgaag atatgggtgg atataaaggg attaaagcag atggtggcaa ggtgaatcaa
    10501 gcgaaacaat tagcggcaaa aatagctaaa gatattgaag catgtcaaaa gcaaacgcaa
    10561 cagctcgctg agtatatcga aggtagtgat tgggaaggac agttcgccaa taaggtgaaa
    10621 gatgtgttac ttattatggc aaagtttcaa gaagaattag tacaaccgat ggctgaccat
    10681 caaaaagcaa ttgataactt aagtcaaaat ctagcgaaat acgatacatt atcaattaag
    10741 caaggacttg atagggtgaa cccatgatga aagatgttaa gcgaatagat tatttttctt
    10801 acgaagaatt aacaatttta ggtggtagta aattgcctct cgtaaatttt gaattgtttg
    10861 atccatcaaa ttttgaagaa gctaaagtgg ctttaattga aaaagaatta gtaacagaga
    10921 atgacaagtt aactgatgca ggttttaaag tggctacatt agtaagagag tatattagcg
    10981 caattgtaaa tattcgaatt aatgatatgt attttgcacc atttagctat gaaaaagatg
    11041 aatatatttt gttaagccgg tttaaaaata atgggtttca aatacgaatt atcaataaag
    11101 acattgcatg gtggtcgatt gtacaatcat atcctttatt gatgagacaa gaaaaatcca
    11161 atgattggga ctttaaacaa attgacgatg aaatattgga gaacttaaat aatgaaagta
    11221 tcgatacgat tgggcgtgtt ttagaaattg aaatctacaa tcatcaaggt gaccctcaac
    11281 aaagtttata taacatttat gaacaaaatg atttgttact cattcgatac ccattaaaag
    11341 ataaagtgct gaatgttcat attggtgtca ttaatacatt tatacgagaa ttatttggat
    11401 tcgatactga tgaaaatcat attaataaag cagaggagta atgacgttga gtggaaaaat
    11461 tagtgttaaa gctgaaacga ttgcacatgt tgtaaaagaa ttggaaagca ttagtcaaaa
    11521 gtatgatgaa atagctcaaa actttggaaa aatagcgcaa ttaaattact acagtagtga
    11581 aaaagctgca cattctatgg aaaatggcta tagtagtgct gcaacagtca ttagtggtct
    11641 caaaggtcca ttgagtacac tcggtggtgg cgtcatgaat tcagcacaaa agttctttga
    11701 agcagatgaa cattggggta cggaatttgc caagctttac tataatattg agggataggt
    11761 gcatgacatg acaaaagata ttgaatatct aacagctgat tatgacaatg aaaagtcatc
    11821 tatccaaagt gtaatagatg caatagaggg tcaagacttc ttagatgtag atacaacaat
    11881 ggatgatgcg gtaagcgatg tcagttcttt agacgaagat ggcgcaatat cattaacaag
    11941 tagtgtagta ggtccacaag gatctaaatt aatggggtat tatcaaaatg agctatatga
    12001 ttatgcatct caattagatt cgaaaatgaa agaaattatt gacacgccat ttatagaaga
    12061 tatagataaa gcattcaaag gtataacgaa tgttaaattg gaaaatatat taataaaaaa
    12121 tggcggtggt catggtagag atacctatgg ggcttctggg aaaattgcaa agggagatgc
    12181 caagaaaagt gacagcgatg tttatagcat cgatgaaata ttaaaatcgg atcaagaatt
    12241 tgtaaaagta attgatcagc attacaaaga aatgaaaaaa gaagataaga aactatctaa
    12301 gagtgatttt gaaaaaatga tgactcaagg tgcttcttgt gattacatga cagtagctga
    12361 agcggaagag atagaagagc aaaagaaaaa agaagaagct atagagattg cagcactagc
    12421 tggtatggta gttttatctt gtattaatcc tgttgctgga gcagtagcta ttggtgctta
    12481 ttccgcttat tcagcagcaa atgcagccac aggaaaaaat attgtaactg gaagaaagct
    12541 atctaaagaa gaacgaatca tggaaggact ttcgcttatt ccattgccag gtatgggctt
    12601 cctcaaaggt gctgggaaaa gtttaatgaa attaggcttc aaaggcggag aaaaatttgc
    12661 agttaaaaca ggattgcaaa agacaatgca acaagcagtt agtcgtattt cacctaaaat
    12721 gggaatgatg aaaaacagtg tgttgaatca atctcgtaac tttgctcaaa atactcatgt
    12781 tggacaaatg ctgagtaaca tgcgtggtca agcaactcat actgttcaac aaagtagaaa
    12841 ttggattgga caacaagcac aaaatgtcaa acgaatagtg aataatggac ttgataaaga
    12901 aatagcacat ccatttaaac aacaacttgc accagcggga atgggtggta taaaatttgc
    12961 tgaaacaact actttgagaa acatgggtca aaacatgaaa cgtgctgtta caccacaaaa
    13021 tcacgtgaca catggtccga aagatagtat ggtgagaagt gaaggtaaac atagtgtaag
    13081 tagccatgaa ataaatttat caaaatatgt tgaatcacca aactacacca aggtcaaata
    13141 tggagagcaa tatgcaagac ttagacctaa gaaactaaaa gcaaatatag aatatacaac
    13201 accaactggt cacatatatc ggactgatca taaagggcgc ataaaagaag tttatgtaga
    13261 caatctttct ctaaaagatg gcggtcgtaa taaccatgca caaagaactg tgggaggaga
    13321 ggatagatta ccagacgatg atggaggtca cttaatcgcc agaatgtttg gtgggtcaaa
    13381 agacatagat aaccttgtag cacaaagtaa atatatcaac cgttcattta aggaaaatgg
    13441 tgattggtac aagttggaaa aactatggga aaaggcaatc aagtctggaa aatcaataga
    13501 aaatattaaa atagaagtta aatataaagg taatagtcaa aggccgacta tatttaaagt
    13561 tgaatatgaa attaatggta aaagaaatat tagaagaata ttaaataagt agaggtgcca
    13621 acatgacatt tgaagagaag cttagcaaaa tatacaatga aattgcgaat gagattagca
    13681 atatgatacc ggtagagtgg gaaaaagtat atacaatggc ttatatagat gatggaggag
    13741 gggaggtatt ctttaattat actaa
//
LOCUS       DAHHSC010000053        13719 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.053, whole genome shotgun sequence.
ACCESSION   DAHHSC010000053 DAHHSC010000000
VERSION     DAHHSC010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 13719)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13719)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13719
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.053"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            complement(<1..160)
                     /locus_tag="NBI57_001186"
     CDS             complement(<1..160)
                     /locus_tag="NBI57_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1822143.1"
                     /translation="MKRIVILENVIVDNPIWLNHVDYLVKSVLLKSIKLRFQEVRKFE
                     ILVKIMNRI"
     gene            complement(288..791)
                     /locus_tag="NBI57_001187"
     CDS             complement(288..791)
                     /locus_tag="NBI57_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HCD1822144.1"
                     /translation="MSKWIIVSDNHTESGVLYQIYEMHPDADVYLHLGDSEFAYDDTE
                     LSLFNRVKGNCDFYPEFENEAVAKYNDVKAFYTHGHLYQVNRTRDLLAEKGLELGCLF
                     AFYGHTHVAKYEYINGVHVINPGSISQSRSSMEETYAEVIIDDQTLHGTINFKNRHHE
                     TISHTTF"
     gene            complement(784..1371)
                     /locus_tag="NBI57_001188"
     CDS             complement(784..1371)
                     /locus_tag="NBI57_001188"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XTP/dITP diphosphatase"
                     /protein_id="HCD1822145.1"
                     /translation="MKEIVIASNNQGKINDFKVIFPDYHVIGISELIPDFDVEETGST
                     FEENAILKSEAAAKALNKTVIADDSGLEVFALNGEPGIYSARYAGENKSDEANIEKLL
                     NKLGNTTDRRAQFVCVISMSGPDMETKVFKGTVSGEIADGKYGENGFGYDPIFYVPKL
                     DKTMAQLSKEQKGQISHRRNAINLLQAFLEGEKNV"
     gene            complement(1383..2183)
                     /gene="racE"
                     /locus_tag="NBI57_001189"
     CDS             complement(1383..2183)
                     /gene="racE"
                     /locus_tag="NBI57_001189"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HCD1822146.1"
                     /translation="MNKPIGVIDSGVGGLTVAKEIMRQLPNETIYYLGDIGRCPYGPR
                     PGEQVKQYTVEIARKLMEFDIKMLVIACNTATAVALEYLQKTLSIPVIGVIEPGARTA
                     IMTTRNQNVLVLGTEGTIKSEAYRTHIKRINPHVEVHGVACPGFVPLVEQMRYSDPTI
                     TSIVIHQTLKRWRNSESDTVILGCTHYPLLYKPIYDYFGGKKTVISSGLETAREVSAL
                     LTFSNEHASYTEHPDHRFFATGDPTHITNIIKEWLNLSVNVERISVND"
     gene            complement(2419..3234)
                     /gene="sdhB"
                     /locus_tag="NBI57_001190"
     CDS             complement(2419..3234)
                     /gene="sdhB"
                     /locus_tag="NBI57_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit"
                     /protein_id="HCD1822147.1"
                     /translation="MTEQSVKNTPQHETQSKPKQKTVKLIIKRQDTSDSKPYEETFEI
                     PYRENLNVIACLMEIRRNPVNIKGEKTTPVVWDMNCLEEVCGACSMVINGRARQSCSA
                     IVDQLEQPIRLEPMNTFPVIRDLQVDRSRMFDNLKRMKAWIPIDGTYDLGPGPRMPEK
                     KRQTAYELSKCMTCGVCLEVCPNVTENNKFVGAQAISQVRLFNLHPTGSMTKDERLNA
                     LMGTGGLQQCGNSQNCVNACPKGIPLTTSIAAMNRETTFHMFKSFFGSDHEVE"
     gene            complement(3234..5000)
                     /gene="sdhA"
                     /locus_tag="NBI57_001191"
     CDS             complement(3234..5000)
                     /gene="sdhA"
                     /locus_tag="NBI57_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HCD1822148.1"
                     /translation="MAEKHLIVVGGGLAGLMSTIKAAEKGAHVDLFSVVPVKRSHSVC
                     AQGGINGAVNTKGEGDSPWIHFDDTVYGGDFLANQPPVKAMTEAAPKIIHLLDRMGVM
                     FNRTNEGLLDFRRFGGTLHHRTAYAGATTGQQLLYALDEQVRAYEVDGLVTKYEGWEF
                     LGIVKGDDDSARGIVAQNMTTAEIETFGSDAVIMATGGPGIIFGKTTNSMINTGSAAS
                     IVYQQGAIYANGEFIQIHPTAIPGDDKLRLMSESARGEGGRIWTYKDGKPWYFLEEKY
                     PDYGNLVPRDIATREIFDVCINQKLGINGENMVYLDLSHKDPHELDVKLGGIIEIYEK
                     FTGDDPRKVPMKIFPAVHYSMGGLYVDYDQMTNIKGLFAAGECDFSQHGGNRLGANSL
                     LSAIYGGTVAGPNAIDYISNIDRSYTDMDESIFEKRKAEEQERFDKLLAMRGTENAYK
                     LHRELGEIMTANVTVVRENEKLLETDKKIVELMKRYEDIDMEDTQTWSNQAVFFTRQL
                     WNMLVLARVITIGAYNRNESRGAHYKPEFPERNDEEWLKTTMASFQGAFEKPQFTYDD
                     VDVSLIPPRKRDYTSKSKGGKK"
     gene            complement(5052..5666)
                     /locus_tag="NBI57_001192"
     CDS             complement(5052..5666)
                     /locus_tag="NBI57_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b558 subunit"
                     /protein_id="HCD1822149.1"
                     /translation="MAQSKNEFYLRRIHSLLGIIPIGAFLVVHLLVNHQATQGAEAFN
                     KASNFMESLPFLIIVEFLFIYIPLLYHGLFGIHIAFTAKENVGHYSIFRNWMFFFQRV
                     SGILTFIFIGIHLWQTRLQKAFYGKEVNYDLMHETLQHPGWAIFYIICIIAVVFHFAN
                     GLWSFLVTWGGLQSPKSQRVFTWVSLIVFLVISYIGVTAIIAFM"
     gene            complement(5990..7771)
                     /gene="uvrC"
                     /locus_tag="NBI57_001193"
     CDS             complement(5990..7771)
                     /gene="uvrC"
                     /locus_tag="NBI57_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499646.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HCD1822150.1"
                     /translation="MEDYKQRIKNKLNVVPMEPGCYLMKDRNDQVIYVGKAKKLRNRL
                     RSYFTGAHDAKTTRLVGEIRRFEFIVTSSETESLLLELNLIKQYQPRYNILLKDDKSY
                     PFIKITKEKYPRLLVTRTVKQGTGKYFGPYPNAYSAQETKKLLDRIYPYRKCDKMPDK
                     LCLYYHIGQCLGPCVYDVDLSKYAQMTKEITDFLNGEDKTILKSLEERMLTASESLDF
                     ERAKEYRDLIQHIQNLTNKQKIMSSDKTIRDVFGYCVDKGWMCIQVFFIRQGNMIKRD
                     TTMIPLQQTEEEEFYTFIGQFYSLNQHILPKEVHVPRNLDKEMIQSVVDTKIVQPARG
                     PKKDMVDLAAHNAKVSLNNKFELISRDESRTIKAIEELGTQMGIQTPIRIEAFDNSNI
                     QGVDPVSAMVTFVDGKPDKKNYRKYKIKTVKGPDDYKSMREVVRRRYSRVLNEGLPLP
                     DLIIVDGGKGHMNGVIDVLQNELGLDIPVAGLQKNDKHQTSELLYGASAEIVPLKKNS
                     QAFYLLHRIQDEVHRFAITFHRQTRQKTGLKSILDDIDGIGNKRKTLLLRSFGSIKKM
                     KEATLEDFKNIGIPENVAKNLHEQLHK"
     gene            7937..8032
                     /locus_tag="NBI57_001194"
     CDS             7937..8032
                     /locus_tag="NBI57_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1822151.1"
                     /translation="MLDYHLTFIDLLIPPFSSRKQYDDLSFNFLY"
     gene            complement(8095..8409)
                     /gene="trxA"
                     /locus_tag="NBI57_001195"
     CDS             complement(8095..8409)
                     /gene="trxA"
                     /locus_tag="NBI57_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HCD1822152.1"
                     /translation="MAIVKVTDADFDSKVESGVQLVDFWATWCGPCKMIAPVLEELAA
                     DYEGKADILKLDVDENPSTAAKYEVMSIPTLIVFKDGQPVDKVVGFQPKENLAEVLDK
                     HL"
     gene            complement(8582..10930)
                     /locus_tag="NBI57_001196"
     CDS             complement(8582..10930)
                     /locus_tag="NBI57_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HCD1822153.1"
                     /translation="MRQKTLDVLEFEKIKSLVANETISDLGLEKVNQMMPATNFETVV
                     FQMEETDEIAQIYNKHRLPSLSGLSKVSAFIHRADIGGVLNVSELNLIKRLIQVQNQF
                     KTFYNQLVEEDEGVKYPILDDKMNQLPVLTDLFHQINETCDTYDLYDNASYELQGIRS
                     KISSTNQRIRQNLDRIVKSQANQKKLSDAIVTVRNERNVIPVKAEYRQDFNGIVHDQS
                     ASGQTLYIEPSSVVEMNNQISRLRHDEAIEKERILTQLTGYVAADKDALLVAEQVMGQ
                     LDFLIAKARYSRSIKGTKPIFKEERTVYLPKAYHPLLNRETVVANTIEFMEDIETVII
                     TGPNTGGKTVTLKTLGLIIVMAQSGLLIPTLDGSQLSVFKNVYCDIGDEQSIEQSLST
                     FSSHMTNIVEILKHADKHSLVLFDELGAGTDPSEGAALAMSILDHVRKIGSLVMATTH
                     YPELKAYSYNREGVMNASVEFDVDTLSPTYKLLMGVPGRSNAFDISKKLGLSLNIINK
                     AKTMIGTDEKEINEMIESLERNYKRVETQRLELDRLVKEAEQVHDDLSKQYQQFQNYE
                     KSLIEEAKEKANQKIKAATKEADDIIKDLRQLREQKGADVKEHELIDKKKRLDDHYEA
                     KSIKQNVQKQKYDKIVAGDEVKVLSYGQKGEVLEIVNDEEAIVQMGIIKMKLPIEDLE
                     KKQKEKVKPTKMVTRQNRQTIKTELDLRGYRYEDALIELDQYLDQAVLSNYEQVYIIH
                     GKGTGALQKGVQQHLKKHKSVSDFRGGMPSEGGFGVTVATLK"
     gene            complement(10940..12652)
                     /gene="polX"
                     /locus_tag="NBI57_001197"
     CDS             complement(10940..12652)
                     /gene="polX"
                     /locus_tag="NBI57_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase/3'-5' exonuclease PolX"
                     /protein_id="HCD1822154.1"
                     /translation="MTKKDVIKLLEQIATYMELKGENTFKISAYRKAAQSLELDERPL
                     DEISDVTELKGIGKGVAEVINDYRETGESQYLQQLQEEVPEGLIPLLKIQGLGSKKIA
                     KLYKELNIVDKASLQVACENGKVSELSGFAKKTEQNILEAVKQLGAKKDRYPIDQMRR
                     LNQEIIDYIDTLNYIDQYSSAGSFRRFKEMSKDLDFIISTDNPKAVQQQLLNIPNKVK
                     EVAVGNTKVSLELAYDDETIGVDFRLIEPRAFYHTLQHFTGSKEHNIRIRQLAKARDE
                     KVSEYGIEQADGTLIQYDSEAKIYEHFNVNFIPPAMREDGSEFDKDLSNIITLDDING
                     DIHMHTTYSDGAFSIRDMVEANIAKGYKFMVITDHSQSLRVANGLQVERLLRQNEEIK
                     ALDKEYSEIDIYSGTEMDILPDGSLDYDDEILAQLDYVIGAIHQSFNQSEEQIMERLA
                     NACRNPYVRHIAHPTGRIIGRRDGYKPNIEQLMALAEETNTVLEINANPHRLDLNAEI
                     VRKYPNVKLTINTDAHHTNHLDFMNYGVATAQKGFVTKDRVINALSREAFKDFIENNI
                     KLKK"
     gene            complement(12725..13246)
                     /locus_tag="NBI57_001198"
     CDS             complement(12725..13246)
                     /locus_tag="NBI57_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HCD1822155.1"
                     /translation="MVIDFIIIIFFVYFVIVGFRRGFWLSMIHLSATIVSLWIASQFY
                     KSIVERLIVFIPYPKTTAFNTTFAFHFNHLQNRFEAIVAFLMITLFCKFILYLIIVTF
                     DKIIAYQNIHIFSRAMGMIVGVFMTIIVLHFTLYLLALYPNEALQHQLKISIVSHSLI
                     FHIPYLSAFTINL"
     gene            complement(13247..13513)
                     /gene="zapA"
                     /locus_tag="NBI57_001199"
     CDS             complement(13247..13513)
                     /gene="zapA"
                     /locus_tag="NBI57_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HCD1822156.1"
                     /translation="MAQFKNKVNVSINDQLFTIVGEDNPEHIRYVAHLVDDKIKELGY
                     KAAGLDTSRKAILTAVNIMHEKVLLEEENRRLKQQIHKLQQREQ"
BASE COUNT         4185 a         2631 c         1900 g         5003 t
ORIGIN      
        1 gtatacgatt catgatttta actaaaattt caaatttcct gacttcttgg aatcttaatt
       61 ttatactttt taataataca cttttaacca aataatcaac atgattcaac catatagggt
      121 tatcaacaat cacattctcc aaaattacaa ttcttttcat aacataaatg tgttattcat
      181 tccaattatc ttataaaatt gtcagaaata cttttaaaca aacattcatg acacgacctc
      241 ctagttaaat cagtctataa actaaaaaaa gccatagcta cttctattta aaaagtagta
      301 tgactgattg tttcgtgatg tcgatttttg aaattgatgg tgccatgtaa agtttgatca
      361 tcaataataa cttcagcata tgtttcttcc attgaacttc tagattgaga tatacttcca
      421 ggattaataa catgaacacc attaatatac tcatattttg ccacatgtgt atgtccataa
      481 aatgcaaaca aacaacctaa ttcaagtcct ttttcagcta ataaatctct tgttcgattg
      541 acttgatata aatgtccatg agtataaaat gctttcacgt cattatattt tgcgaccgct
      601 tcattttcaa attctgggta aaaatcacaa ttgcctttta ctctattaaa taagctaagt
      661 tccgtatcat catacgcgaa ttctgaatct cctaaatgta aatatacatc tgcatctggg
      721 tgcatttcat aaatttgata taaaacgcct gattcagtat ggttatcact cacaataatc
      781 catttagaca tttttttcac cttcaagaaa agcttgaagt aaattaatcg catttcgtct
      841 atgactaatt tgcccttttt gttcttttga aagttgagcc atggttttat ctaatttcgg
      901 tacataaaat atcggatcat atccgaaacc attttcgcca tattttccat ctgcaatttc
      961 acctgaaaca gtacctttaa atacttttgt ttccatatca gggccactca tacttatgac
     1021 acaaacaaat tgcgcacgac gatcagttgt attaccaagc ttatttaata atttttcaat
     1081 atttgcttca tcgcttttat tttcaccagc ataacgtgca gagtatatac ctggctcacc
     1141 atttaatgca aaaacttcta gtccactgtc atcagctatg acagttttat tcaatgcttt
     1201 tgcagcagct tctgatttta atatagcatt ttcttcaaat gttgatcctg tttcttccac
     1261 atcaaaatct ggtattagtt ctgaaatacc tattacgtgg taatctggaa atattacttt
     1321 aaagtcattt attttccctt gattattcga tgcaataaca atctctttca ttaaaaatcc
     1381 tcctagtcat tcactgatat acgttccaca ttgacagata aatttaacca ctctttgata
     1441 atgttagtaa tgtgagtagg atcacctgtt gcaaaaaatc gatgatctgg atgttcagta
     1501 taacttgcat gttcattact aaatgttagc aatgcactaa cttcacgagc cgtttctaat
     1561 ccagacgaaa tcactgtctt tttaccacca aaataatcat agataggttt atagagcaat
     1621 ggatagtggg tacatcctaa aatgacagta tcagactcac tattacgcca acgtttcagt
     1681 gtttgatgaa tgacaatgct tgtaattgtt ggatcactat atctcatttg ttctacaagt
     1741 ggcacaaaac ctggacaggc aacgccatgt acctctacat gtggattgat acgtttaata
     1801 tgcgtacgat atgcttcaga tttaattgtg ccttcagttc ctagtactaa tacattttga
     1861 tttctagtag tcattattgc tgttctagca cctggttcaa ttacaccaat cactgggatt
     1921 gataaggtct tttgtaaata ttctaaagct acagcagttg ctgtattaca agcaatcacg
     1981 agcattttta tatcaaattc cattaattta cgagcgattt caactgtata ttgttttact
     2041 tgttctcctg gtcttggccc atatggacat cgtccaatat cacctaagta ataaatcgtc
     2101 tcatttggca actgacgcat aatttcttta gctactgtca aacctccgac accagagtct
     2161 attacaccta ttggtttatt catattcggt catccttact tttaatttaa tttttagttt
     2221 atcataacta agcattggat tttagtatta tgcactgtgt ttaccatttt tgtcattata
     2281 atatttattt taaatcagcc cactatcata ttgtcatgta atctgcttat taaaaaaatc
     2341 ccttccaagt tattgtgtat ctccattcaa tttaattttg aaaggaacat aacattttaa
     2401 ctcaaaaggg attaattttt attctacttc atggtctgaa ccaaagaatg atttaaacat
     2461 gtggaatgtt gtttctctgt tcattgctgc aatggatgtt gttaatggaa tacctttagg
     2521 gcaagcatta acacagtttt gtgaattacc acactgctgt aagccaccag tacccattaa
     2581 tgcatttaaa cgttcatctt tagtcataga tcctgttggg tgcaaattaa acaaacgaac
     2641 ttgcgagatt gcttgtgcac caacgaattt attattttca gtaacattag gacaaacctc
     2701 taaacataca ccacatgtca tacatttaga taattcataa gctgtttgac gttttttctc
     2761 tggcatacgt ggtcccggac ctaaatcata cgttccatca attgggatcc atgctttcat
     2821 acgttttaag ttatcgaaca ttctagaacg atcaacttgt aagtcacgga taactgggaa
     2881 agtattcatt ggctctaaac gaataggttg ttctaattga tcaacaatcg cagaacaaga
     2941 ttgtcttgca cgaccattga taaccataga acatgctcca catacttctt ctaagcagtt
     3001 catatcccag acaacaggtg ttgttttttc acctttaata ttaactgggt tacgtctaat
     3061 ttccattaaa caagcaatga cgtttaaatt ttcacgatat ggaatttcaa atgtttcttc
     3121 ataaggctta gaatcacttg tatcttgtcg tttaataatt aattttactg ttttttgttt
     3181 cggtttagat tgtgtttcat gttgtggagt gtttttcact gattgttcag tcattatttt
     3241 ttaccccctt tagacttact tgtgtaatca cgtttacgag gtggtattaa actcacatcg
     3301 acgtcatcat aagtaaactg tggtttttca aatgcgcctt ggaatgaggc cattgtcgtt
     3361 tttaaccact cttcatcatt acgctctggg aattctggtt tataatgggc accgcgtgat
     3421 tcgttacggt tatatgcacc aatcgtaata acacgtgcaa gtactaacat gttccatagt
     3481 tgacgggtaa agaataccgc ttggttactc caagtttgag tatcttccat atcaatatct
     3541 tcataacgtt tcatcaattc aacaatcttt ttatctgttt ctaacagttt ttcattttca
     3601 cgaacaacag ttacatttgc tgtcataatt tcaccaagtt cacggtgtaa tttataggca
     3661 ttttctgtac cgcgcatagc taataattta tcaaaacgtt cttgctcttc agctttacgt
     3721 ttttcaaaaa tactttcgtc catatcagta tatgatcgat caatatttga aatataatca
     3781 atcgcgtttg gacctgctac tgtaccacca taaatcgctg ataacaatga attggcacct
     3841 aagcggttac caccatgttg agagaagtca cattctccag ctgcaaataa ccctttaata
     3901 tttgtcattt gatcataatc tacatataga ccacccattg aatagtgaac agctgggaaa
     3961 atcttcattg gtactttgcg tgggtcatca ccagtgaatt tttcataaat ttcaatgata
     4021 ccacctagtt ttacatctaa ctcatgtgga tctttatgtg acaaatcaag atataccatg
     4081 ttttcgccat ttatacctaa tttttggtta atacatacat cgaaaatttc acgcgttgcg
     4141 atatcacgag gtactaagtt accataatca ggatatttct cttctaagaa gtaccaaggc
     4201 ttaccatctt tatatgtcca aattcgtcca ccttcaccac gtgctgattc actcattagt
     4261 cgcagtttat catcaccagg gattgcagta ggatgaattt gaatgaactc accattagca
     4321 taaatagcgc cttgttggta aacaatggaa gccgctgatc ctgtattaat cattgagttt
     4381 gttgttttac cgaaaataat accagggcca cccgttgcca taataactgc atctgaacca
     4441 aatgtttcaa tctcagcagt tgtcatattt tgtgcaacga tacctcttgc actatcatcg
     4501 tcacctttaa ctatgccaag aaattcccat ccttcatact tcgtaactaa tccatctact
     4561 tcatatgcac gaacttgttc atccaatgca tataataatt gttgtccagt tgttgcccct
     4621 gcatatgctg ttctgtggtg taatgtacca ccgaaacgtc taaaatctaa tagaccttca
     4681 tttgttctat tgaacattac acccatacgg tctaataaat gaataatttt aggtgctgcc
     4741 tctgtcatcg ctttaacagg tggttggttt gcaaggaagt cgccaccata cactgtatca
     4801 tcaaagtgaa tccaaggaga atcgccttcc cctttagtat tgaccgcacc attaatgcca
     4861 ccttgggcac aaacagagtg cgaacgcttt actggtacaa ctgagaacaa atctacatgt
     4921 gcaccttttt ctgccgcttt aattgttgac attaagcccg ctaggccacc tccgacaaca
     4981 ataagatgtt tctctgccat aaaaatttca ctcccctaaa ttttcaatct atatttgtta
     5041 aatgcgatgt attacataaa ggcaataatt gcagtaacac caatatacga aataactaaa
     5101 aatacgatta atgaaaccca tgtaaatact cgttgtgatt ttggagattg aagtccaccc
     5161 caagtaacta agaatgacca taagccattt gcaaagtgga acacaacagc aataatacaa
     5221 ataatataaa atattgccca tccaggatgt tgcaatgttt cgtgcattaa atcgtaattc
     5281 acttctttgc cgtaaaatgc tttttgtaaa cgtgtttgcc ataaatggat accaataaag
     5341 ataaatgtta agataccact cactctttgg aagaagaaca tccagtttct aaaaatcgag
     5401 taatgtccaa cattttcttt tgctgtaaat gcaatgtgta taccaaacaa accgtgatat
     5461 aacaacggaa tgtatataaa taaaaattct acaataatta gaaatggtaa tgattccata
     5521 aagttagatg ccttattaaa cgcttcagca ccttgtgttg cttggtgatt cactaataaa
     5581 tgaacgacca aaaatgcacc tattgggata atacctaata acgagtgaat acgtcttaga
     5641 taaaattcat tttttgattg agccaaaagg agtcccccct gtgaacgaat atttaattta
     5701 ttgagctatt tatattaaac gtacgcttaa ccccctaaag tgatatcgtt tctagcgttg
     5761 tcattatcat taagcgaaac attttaaaga caaatacaca ctgtacgatc accaaactgc
     5821 atgtcgaaca atgtaacatt tggattcgat atttaaaatt gcttgtgatg ataaactttc
     5881 tcatttagaa aacgcttcca cgtacattca aaaaaataac tttgttaacc atattgtaac
     5941 attatttcat atattttggg gcatgagaat gattctcacg cccagtaatt tatttatgca
     6001 attgttcatg taggttcttt gcgacgtttt caggaatacc tatattttta aaatcttcaa
     6061 gtgtagcttc cttcattttc ttgattgaac cgaatgaacg caataataat gttttacgtt
     6121 tgttaccgat accatctata tcatcaagta ttgatttcaa gcctgtcttt tgacgtgttt
     6181 gtctatgaaa tgtgattgcg aatctgtgaa cctcatcttg gatacgatgc aacaaataaa
     6241 atgcctggct atttttcttc agtggtacaa tttctgcact agcgccatat aataattcag
     6301 atgtttggtg tttatcattt ttctgcaaac ctgcaacagg gatatcaaga cctaattcgt
     6361 tttgtagcac atcaataacc ccgttcatat gtcctttacc accatcgact attattaaat
     6421 caggtaatgg taatccttcg tttaaaacgc gagaatatcg tcgtcttact acttctctca
     6481 ttgatttgta atcatctgga cctttaaccg ttttgatttt atactttcta taatttttct
     6541 tatctggttt accgtcgaca aatgtaacca ttgctgacac tggatccaca ccttgaatat
     6601 tagaattatc gaatgcttca attctaattg gtgtttgaat tcccatttgt gttccaagtt
     6661 cttcaatagc tttaatcgtt cttgactcat cacgtgatat taattcaaat ttattattta
     6721 aggatacttt agcgttatgt gcagctaggt caaccatatc ttttttggga cctcgcgcgg
     6781 gttgaacgat tttagtgtcc acaacagatt gaatcatttc tttatccaaa ttacgtggta
     6841 catgaacttc cttaggtaaa atatgttggt ttaagctata aaattgtcca ataaatgtat
     6901 aaaattcttc ttcttctgtt tgctgtaatg gaatcatcgt tgtatctcgc tttatcatat
     6961 taccttgtcg tataaagaaa acttggatac acatccatcc tttatcaaca caataaccaa
     7021 agacatcacg aatcgtttta tctgatgaca taattttttg tttgtttgtc agattttgaa
     7081 tatgttgaat taaatctcta tattctttag cccgttcaaa atcaagtgat tcacttgcag
     7141 ttaacattcg ctcttctaaa ctttttaaaa ttgttttgtc ttccccattc agaaaatcag
     7201 taatttcctt cgtcatttgt gcgtatttac tcaaatcaac gtcatataca catggtccta
     7261 aacattgtcc aatatggtaa taaagacata atttatctgg catcttatca catttgcgat
     7321 atggatatat tctgtctaat aactttttag tttcttgagc agaatatgca ttcggatacg
     7381 gtccgaaata tttgccagta ccttgtttta cagttctcgt cactagtagt ctaggatatt
     7441 tctccttcgt aattttaata aatggataac ttttatcatc ctttaataat atattatatc
     7501 ttggttgata ttgttttatc agattcaatt ccagtaaaag tgactctgtt tcacttgacg
     7561 tgacaataaa ctcaaaacga cgtatttcac caaccagtct cgttgtttta gcatcatgag
     7621 cacccgtaaa atatgatctc aatcgatttc ttagcttttt agctttgcca acatatatca
     7681 cttgatcatt acgatctttc attaaatagc agcctggttc cataggtacg acatttaatt
     7741 tatttttaat tcgttgctta tagtcttcca aaaatatccc cccttctaat tttaaaatga
     7801 atgatgctac aaaagtgctt caaatttatc accactaatt ctagctcata atcaacatct
     7861 tcgagtatga cttttcatgc acatcatgta atattgaaca tggttttatc aaaccaactg
     7921 ctacaaatct atcagcatgc tagattatca tttaacgttc atagatttat taatcccacc
     7981 attctcaagt cgtaaacaat atgatgactt atcattcaac tttttatatt aaaaacacaa
     8041 acaaaaactg gagcaattca ttaaagaaat gccccagtcg tcattggtta taacttataa
     8101 atgtttatct aaaacttcag ctaagttttc ttttggttgg aaaccaacaa ctttatcaac
     8161 tggttgaccg tctttaaaga cgattaatgt tggaatactc atcacttcat atttagctgc
     8221 agttgatgga ttttcatcaa catctaattt taaaatgtca gctttacctt catagtcagc
     8281 tgctaattct tctaataccg gagcgatcat tttacatgga ccacaccatg ttgcccaaaa
     8341 atctactagt tgtacaccag attctacttt tgaatcaaaa tctgcatctg ttacttttac
     8401 gattgccata aatgccaatc ctccttaatt attattatga tgttcaaatt atatcagata
     8461 acatgcttca tgtatttcta tttgctcaat ttttatgctt atttcaaata acatgtttga
     8521 aatgtattct tgttgcttta cattatttta actgcagcta tttaatttat caaattataa
     8581 tttattttag tgttgcaacg gtaacgccaa atccaccttc gcttggcata ccacctctaa
     8641 agtcactaac acttttatgc ttcttcaaat gttgttgtac acctttttga agtgcacctg
     8701 tacctttacc atgaatgata taaacttgtt cgtaattact taaaacggct tgatctaaat
     8761 attgatctag ttcaattaaa gcatcctcat aacgatagcc tcgtaagtca agttcagttt
     8821 taattgtttg acgattttga cgtgtaacca ttttcgttgg cttaactttt tctttttgtt
     8881 ttttctctaa atcttcaata ggtaacttca ttttaataat tcccatttga acaattgctt
     8941 cttcatcatt gacaatttct aaaacttcac ccttttgacc gtaagataat acttttactt
     9001 catcaccagc aacaatttta tcgtattttt gcttttgtac attttgcttt atagatttcg
     9061 cttcataatg atcatctaat cgtttcttct tatcaatcaa ttcatgttct ttaacatctg
     9121 cacctttttg ttcacgcaat tgtcttaagt ctttaataat atcgtcagct tcttttgttg
     9181 cagctttaat cttctgattt gctttttctt tcgcttcctc tattagagac ttttcataat
     9241 tttggaattg ttgatactgc ttagataaat catcatgcac ttgctccgct tcttttacaa
     9301 gacggtccag ttctaacctc tgtgtctcta cacgtttata attacgctct aatgattcaa
     9361 tcatttcatt tatttctttt tcatcagtac caatcatcgt cttagcctta ttaataatat
     9421 tcaaactaag acctaacttt ttagaaatgt caaaagcatt tgaacgaccc ggcacaccca
     9481 ttaataactt atacgttgga ctcaaagtat ctacatcaaa ttctacactc gcattcataa
     9541 cgccttctcg attataacta tatgctttaa gttcaggata gtgcgtcgtt gccattacta
     9601 gagaaccaat ttttctaaca tgatctaaaa tgctcattgc taatgcagca ccttcacttg
     9661 gatctgtacc tgcacctaat tcatcaaata aaactaaact atgtttgtct gcatgcttta
     9721 aaatttcaac tatattcgtc atatgagatg aaaaagttga taatgattgt tctattgatt
     9781 gttcatctcc gatatcgcaa tatacatttt taaatacact taactgacta ccatcaagtg
     9841 tgggaatcaa caatcctgat tgagccataa caataattaa acctaatgtt tttaatgtta
     9901 cagttttacc acctgtattc ggtcctgtaa taattaccgt ttcaatatct tccataaatt
     9961 cgatggtatt agctacaaca gtctcacgat ttaataatgg atggtatgct ttaggtaaat
    10021 atacagtacg ttcctcttta aatatcggct ttgttccttt aatacttcta ctatatctcg
    10081 cttttgcgat taaaaaatct aactgcccca tgacttgttc tgccacaagt agtgcatctt
    10141 tgtccgcagc cacataacca gttagttgcg ttaaaatgcg ttctttttca attgcttcgt
    10201 catgacgtaa tcgactaatt tgattattca tttcaacaac tgatgatggc tcaatataca
    10261 atgtttgtcc tgaagcagat tgatcatgta caatcccatt aaaatcttgt cgatattcag
    10321 ctttgacagg tataacgttt ctttcattcc taactgttac aatagcatct gataattttt
    10381 tctgatttgc ttggctttta acaatacggt ccaaattttg tctaatacgt tgattcgtgc
    10441 tagaaatttt acttctaatc ccttgcaatt cataactcgc attatcatat aaatcatacg
    10501 tatcgcatgt ttcatttatt tgatgaaaaa gatcagttaa cacaggtaat tgattcatct
    10561 tgtcatctaa tattgggtat ttaacacctt catcttcttc aaccaattga ttataaaatg
    10621 tcttaaattg attttgtact tgaattaatc tttttatcaa gttaagctct gatacattta
    10681 aaacgccgcc aatatcagcg cgatgaatga atgctgatac tttagataag ccactcaagc
    10741 ttggtaaacg atgcttatta tagatttgag caatctcatc cgtttcttcc atttgaaaaa
    10801 caaccgtttc aaaattagta gctggcatca tttgattgac cttttccaag cctaagtcac
    10861 taatagtttc attggcaacg agtgatttta ttttttcaaa ttctaagacg tctaatgttt
    10921 tttgtctcat aaaatccctc tatttcttaa gttttatatt attttcaata aagtctttaa
    10981 aagcttcacg cgataatgcg ttaatcactc tatcttttgt tacaaatcct ttttgcgcag
    11041 ttgctacgcc ataattcata aaatctaaat gatttgtatg atgcgcatca gtgttaatag
    11101 ttaatttcac atttggatat ttacgaacga tttcagcgtt cagatccagt cgatgtggat
    11161 tggcattaat ttctaatact gtattcgttt cttcagctaa tgccattaat tgttcaatat
    11221 tcggtttata accatctctt ctacctataa tacgccctgt tggatgcgct atatgtcgca
    11281 cgtatggatt gcgacatgca ttagctaatc gttccataat ttgttcttct gattggttaa
    11341 agctttgatg aatagctcca attacataat caagttgtgc taaaatttca tcatcataat
    11401 ccagcgagcc atcaggtaat atatccattt ctgtacctga ataaatatca atttcactat
    11461 attctttatc taaagcctta atttcttcgt tttgtcttaa aagtctttcc acttgtaagc
    11521 cattagcaac acgtaaactt tgtgaatgat cagtaattac catgaattta taaccttttg
    11581 cgatatttgc ttctaccatg tctcgaatag aaaacgcacc atcactatac gttgtatgca
    11641 tatgaatatc accattaata tcatctaatg taatgatatt acttagatct ttatcaaatt
    11701 cgctaccatc ttctcgcata gcaggtggta taaaattcac attaaaatgt tcatatatct
    11761 tggcttcact atcatattga attaatgtac catcagcttg ttcaattcca tattcactta
    11821 ctttttcatc acgtgcttta gcaagttgtc gaattcttat attatgttct tttgacccag
    11881 taaaatgttg caatgtatga taaaaagccc ttggttcaat taatcgaaaa tcgacaccaa
    11941 tcgtttcatc atcatacgct aattctaatg aaacttttgt gttccccact gcaacttctt
    12001 ttactttatt gggaatattt aataattgct gctgcactgc ttttgggtta tcggtactta
    12061 ttatgaaatc taaatctttg ctcatttctt taaaacgacg gaagcttcct gcagatgaat
    12121 attgatcgat ataatttaat gtatctatat aatcaatgat ttcttgatta agtcttctca
    12181 tttgatcaat tggatatcta tctttcttag caccaagttg tttcacagct tctaatatgt
    12241 tttgttccgt tttcttagca aatccgctta attcactaac ttttccattt tcacaagcaa
    12301 cttgaagtga cgctttatca acaatattca actctttata tagcttagca attttcttgc
    12361 ttccaagtcc ttgaattttc aaaagtggaa taagaccttc cggaacttct tcctgtaatt
    12421 gctgtaaata ctgagattca ccggtctcac ggtaatcatt gattacttct gcaacacctt
    12481 taccaatgcc ttttaactcc gttacatcag atatttcatc taatggtcgt tcatctaatt
    12541 caagactttg agctgctttt cgatacgctg atattttaaa agtattttcc ccttttaatt
    12601 ccatataagt agcaatttgt tctaatagtt tgataacatc tttttttgtc ataataacac
    12661 tccataaaaa gaagaccagg acgtatcatt aatatatacc tttgtcctga cctcttatga
    12721 taatttataa attaatggtg aaagccgata aatatgggat gtgaaaaatc aatgaatgac
    12781 tcacaataga tattttaagc tgatgttgta atgcttcgtt aggatataat gccaatagat
    12841 ataacgtaaa gtgtaagaca attatcgtca taaacacacc aactatcatt cccattgcac
    12901 gactgaaaat atgaatgttt tgatacgcta ttattttatc aaaagttacg ataattagat
    12961 ataaaatgaa cttacaaaac aatgtaatca ttaaaaaagc tacaatcgct tcaaatcgat
    13021 tttgtagatg attaaaatga aacgcaaaag ttgtattaaa tgctgttgtt ttaggatatg
    13081 gaataaatac aattaatctt tctacaatag atttgtaaaa ttgactggca atccacaatg
    13141 atacaatcgt tgcactcaaa tgtatcatag ataaccaaaa acctcgtctg aatccaacga
    13201 tgacaaaata cacaaagaaa atgattatga taaaatcaat gaccatttat tgctcacgct
    13261 gctgcaattt gtgaatttgt tgtttcaaac gtcgattttc ttcttctagt agtacttttt
    13321 catgcataat attcacagca gttagtattg cttttcttga agtatctaaa cctgctgctt
    13381 tataccctaa ttcttttatt ttatcatcaa ctaaatgtgc tacatatcgt atgtgctctg
    13441 ggttatcttc cccaacaatt gtaaaaagct gatcattaat tgatacattt accttgtttt
    13501 taaactgtgc catttataat ttctcctgat ccttttttta aaatctaaat tcacgttata
    13561 aaatatgact ggatagtttg tctgaatttg atactaatat tgttatattg caattatgat
    13621 aaaacaacaa cacaatctct atagatgact taatgttctt tttataatga aataatgtaa
    13681 agaattttct attcaatact ttatcatgtt taaattgtg
//
LOCUS       DAHHSC010000054        13698 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.054, whole genome shotgun sequence.
ACCESSION   DAHHSC010000054 DAHHSC010000000
VERSION     DAHHSC010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 13698)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13698)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13698
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.054"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            312..1007
                     /gene="sceD"
                     /locus_tag="NBI57_001200"
     CDS             312..1007
                     /gene="sceD"
                     /locus_tag="NBI57_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase SceD"
                     /protein_id="HCD1822157.1"
                     /translation="MKKTLLASSLAVGLGIVAGNAGHEAHASEADLNKASLAQMAQSN
                     DQTLNQKPIEAGAYNYTFDYEGFTYHFESDGTHFAWNYHATGANGANMSAQAPATNNV
                     EPSAVQANQVQSQEVEAPQNAQTQQPQASTSNNSQVTATPTESKASEGSSVNVNAHLK
                     QIAQRESGGNIHAVNPTSGAAGKYQFLQSTWDSVAPAKYKGVSPANAPESVQDAAAVK
                     LYNTGGAGHWVTA"
     regulatory      1212..1314
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            1394..2083
                     /gene="tenA"
                     /locus_tag="NBI57_001201"
     CDS             1394..2083
                     /gene="tenA"
                     /locus_tag="NBI57_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiaminase II"
                     /protein_id="HCD1822158.1"
                     /translation="MEFSQKLYQAAKPIINDIYEDDFIQKMLSGDIGADALRHYLKAD
                     AAYLKEFTNLYALLIPKMNSMNDVKFLVEQIEFMVEGEVLAHDILAQIVGESYEEIIK
                     TKVWPPSGDHYIKHMYFQAHSRENAIYTIAAMAPCPYIYAELAKRSQSDHKLNREKDT
                     AKWFDFYSTEMDDIINVFEALMNKLAESMSDKELEQVKQVFLESCIHERRFFNMAMTL
                     EQWEFGGKVND"
     gene            2076..2906
                     /gene="thiD"
                     /locus_tag="NBI57_001202"
     CDS             2076..2906
                     /gene="thiD"
                     /locus_tag="NBI57_001202"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HCD1822159.1"
                     /translation="MIKPKIALTIAGTDPTGGAGVMADLKSFHSCGVYGMGVVTSIVA
                     QNTLGVQHIHNLNHQWVDEQLDSVFNDTLPHAIKTGMIATADTMETIRHYLMQHESIP
                     YVIDPVMLAKSGDSLMDNDTKQNLQHTLLPLADVVTPNLPEAEEITGLTIDSEEKIMQ
                     AGRIFINEIGSKGIIIKGGHSNDTDIAKDYLFTNEGVQTFENERFKTKHTHGTGCTFS
                     AVITAELAKGRPLFEAVHKAKKFISMSIQYTPEIGRGRGPVNHFAYLKKEGLDDELSK
                     "
     gene            2890..3681
                     /gene="thiM"
                     /locus_tag="NBI57_001203"
     CDS             2890..3681
                     /gene="thiM"
                     /locus_tag="NBI57_001203"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HCD1822160.1"
                     /translation="MNYLNKIRIENPLTICYTNDVVKNFTANGLLSIGASPAMSEAPE
                     EAEEFYKVAQALLINIGTLTAQNEQDIIAIAQTANEAGLPIVFDPVAVGASTYRKQFC
                     KLLLKSAKVSVIKGNASEILALIDDTATMKGTDSDANLDAVAIAKKAYAIYKTAIVIT
                     GKEDVIVQDNKAIVLANGSPLLARVTGAGCLLGGVIAGFLFRETEPDIEALIEAVSVF
                     NIAAEVAAENENCGGPGTFSPLLLDTLYHLNETTYQQRIRIQEVE"
     gene            3683..4324
                     /locus_tag="NBI57_001204"
     CDS             3683..4324
                     /locus_tag="NBI57_001204"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HCD1822161.1"
                     /translation="MFNQSYLNVYFICGTSDVPSHRTIHEVLEAALKAGITLFQFREK
                     GESALKGNDKLVLAKELQHLCHQYDVPFIVNDDVSLAKEINADGIHVGQDDAKVKEIA
                     QYFTDKIIGLSISDLDEYAKSDLTHVDYIGVGPIYPTPSKHDAHIPVGPEMIATFKEM
                     NPQLPIVAIGGINTNNVAPIVEAGANGISVISAISKSENIEKTVNRFKDFFNN"
     gene            4410..5282
                     /gene="yidC"
                     /locus_tag="NBI57_001205"
     CDS             4410..5282
                     /gene="yidC"
                     /locus_tag="NBI57_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HCD1822162.1"
                     /translation="MKKKALLPLFLGIMVFLAGCDYSKPEKRSGFFYNTFVDPMKNVL
                     DWLGNNLLNDNYGLAIIILVLVIRIILLPFMLSNYKNSHMMRQKMKVAKPEVEKIQEK
                     VKRARTQEEKMAANQELMQVYKKYDMNPIKSMLGCLPMLIQLPIIMGLYFVLKDQLVD
                     GLFKYPHFLWFDLGRPDIWITIIAGVLYFIQAYVSSKTMPDEQRQMGYMMMVISPIMI
                     IWISLSSASALGLYWSVSAAFLVVQTHFANIYYEKVAKKEVQPFIEAYEREHNGGSNK
                     KGKNTQVVSKKKKK"
     gene            complement(6195..6842)
                     /locus_tag="NBI57_001206"
     CDS             complement(6195..6842)
                     /locus_tag="NBI57_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HCD1822163.1"
                     /translation="MQQSDVISAAKKYMESIHQNDYTGHDIAHVYRVTALAKSIAENE
                     GVNDTLVIELACLLHDTVDEKVVDANKQYVELKSFLSSLSLSTEDQEHILFIINNMSY
                     RNGKNDHVTLSLEGQIVRDADRLDAIGAIGVARTFQFAGHFGEPMWTEHMSLDKINDD
                     LVEQLPPSAIKHFFEKLLKLESLMHTDTAKMIAKERHDFMMMYLKQFFTEWNYHD"
     gene            complement(6870..8354)
                     /gene="cls"
                     /locus_tag="NBI57_001207"
     CDS             complement(6870..8354)
                     /gene="cls"
                     /locus_tag="NBI57_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HCD1822164.1"
                     /translation="MIELLSIALKHSNIILNSIFIGAFILNLLFAFTIIFMERRSANS
                     IWAWLLVLVFLPLFGFILYLLLGRQIQRDQIFKIDKEDKKGLELIVDEQLAALKNENF
                     SNSNYQIVKFKEMIQMLLYNNAAFLTTDNDLKIYTDGQEKFDDLIQDIRNATDYIHFQ
                     YYIIQNDELGRTILNELGKKAEQGVEVKILYDDMGSRGLRKKGLRPFRNKSGHAEAFF
                     PSKLPLINLRMNNRNHRKIVVIDGQIGYVGGFNVGDEYLGKSKKFGYWRDTHLRIVGD
                     AVNALQLRFILDWNSQATRDHISYDDRYFPDVNSGGTIGVQIASSGPDEEWEQIKYGY
                     LKMISSAKKSIYIQSPYFIPDQAFLDSIKIAALGGVDVNIMIPNKPDHPFVFWATLKN
                     AASLLDAGVKVFHYDNGFLHSKTLVIDDEIASVGTANMDHRSFTLNFEVNAFIYDQQI
                     AKKLKQAFIDDLAVSSELTKARYAKRSLWIKFKEGISQLLSPIL"
     gene            8520..8813
                     /gene="csoR"
                     /locus_tag="NBI57_001208"
     CDS             8520..8813
                     /gene="csoR"
                     /locus_tag="NBI57_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-sensing transcriptional repressor CsoR"
                     /protein_id="HCD1822165.1"
                     /translation="MTEQDNAHHSEQIKTNLKSRLNRIEGQVRAINRMIEEDVYCDDV
                     LTQIRATRSALNSVAIKLLEQHMKSCIMNKVNQGAQEEAMEELLVTFQKLIKD"
     gene            8825..9034
                     /locus_tag="NBI57_001209"
     CDS             8825..9034
                     /locus_tag="NBI57_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HCD1822166.1"
                     /translation="MIHQNTIYTAGIETEEQVSQLTERISNMIGVHQVNINIIDGQVT
                     VSYETPANLNSIEKEIYDEGYKIVF"
     gene            9162..9299
                     /locus_tag="NBI57_001210"
     CDS             9162..9299
                     /locus_tag="NBI57_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1822167.1"
                     /translation="MKRPEKIQNVVKLLSSLGVNIKKTKSRLDIINTLPASNKVSHEL
                     K"
     gene            complement(9563..10765)
                     /locus_tag="NBI57_001211"
     CDS             complement(9563..10765)
                     /locus_tag="NBI57_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HCD1822168.1"
                     /translation="MNYSSRQQPDKHWLRKVDWVLVATIAVLAIFSVLLINSAMGGGQ
                     YSANFGIRQIFYYILGAIFAGIIMFISPKKIKHYTYLLYFLICLLLIGLLVIPESPIT
                     PIINGAKSWYTFGPISIQPSEFMKIILILALARVVSRHNQFTFNKSFQSDLLLFFKII
                     GVSLVPSILILLQNDLGTTLVLAAIIAGVMLVSGITWRILAPIFITGIVGAMTVILGI
                     LYAPALIENLLGVQLYQMGRINSWLDPYTYSSGDGYHLTESLKAIGSGQLLGKGYNHG
                     EVYIPENHTDFIFSVIGEELGFIGSVILILIFLFLIFHLIRLAAKIEDQFNKIFIVGF
                     VTLLVFHILQNIGMTIQLLPITGIPLPFISYGGSALWSMMTGIGIVLSIYYHEPKRYV
                     DLYHPKSN"
     gene            11083..12153
                     /locus_tag="NBI57_001212"
     CDS             11083..12153
                     /locus_tag="NBI57_001212"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HCD1822169.1"
                     /translation="MTKENICIVFGGKSAEHEVSILTAQNVLNAIDKDKYHVDIIYIT
                     NDGDWRKQNNITAEIKSTDELHLENGEALEISQLLKESSSGQPYDAVFPLLHGPNGED
                     GTIQGLFEVLDVPYVGNGVLSAASSMDKLVMKQLFEHRGLPQLPYISFLRSEYEKYEH
                     NILKLVNDKLNYPVFVKPANLGSSVGISKCNNEAELKEGIKEAFQFDRKLVIEQGVNA
                     REIEVAVLGNDYPEATWPGEVVKDVAFYDYKSKYKDGKVQLQIPADLDEDVQLTLRNM
                     ALEAFKATDCSGLVRADFFVTEDNQIYINETNAMPGFTAFSMYPKLWENMGLSYPELI
                     TKLIELAKERHQDKQKNKYKID"
     gene            12168..13526
                     /locus_tag="NBI57_001213"
     CDS             12168..13526
                     /locus_tag="NBI57_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HCD1822170.1"
                     /translation="MINVTLKQIQSWIPCEIEDQFLNQEINGVTIDSRAISKNMLFIP
                     FKGENVDGHRFVSKALQDGAGAAFYQRGTPIDENVSGPIIWVEDTLTALQQLAQAYLR
                     HVNPKVIAVTGSNGKTTTKDMIESVLHTEFKVKKTQGNYNNEIGLPLTILELDNDTEI
                     SILEMGMSGFHEIEFLSNLAQPDIAVITNIGESHMQDLGSREGIAKAKSEITIGLKDN
                     GTFIYDGDEPLLKPHVKEVENAKCISIGVATDNALVCSVDDRDTTGISFTINNKEHYD
                     LPILGKHNMKNATIAIAVGHELGLTYNTIYQNLKNVSLTGMRMEQHTLENDITVINDA
                     YNASPTSMRAAIDTLGTLTGRRILILGDVLELGENSKEMHIGVGNYLEEKHIDVLYTF
                     GNEAKYIYDSGQQHVEKAQHFNSKDDMIEVLTSDLKAHDRVLVKGSRGMKLEEVVNAL
                     IS"
BASE COUNT         4927 a         2153 c         2470 g         4148 t
ORIGIN      
        1 actcaattaa aaacgactta catgactagt actgttgtgt aggtcgtctt tttgtgtagt
       61 ttttctaata gaaaatagac atagattgca aaacgattac ataaaaaatt gcaaataaaa
      121 aacattttta ctgtcatgac aattctcgat agaatcatat ctgaaattat gggggatttc
      181 gttgtaatca ctgtgtaaaa aacaaggcta tttttttatt ttgttgtaac ctgcacggat
      241 tttatagatg ttacagtagt ctctgtaaaa cttttagaga aacaaattac ttgtaggagg
      301 attttaagat tatgaagaaa acattactcg catcatcatt agcagtaggt ttaggaatcg
      361 tagcaggaaa tgcaggtcac gaagcccatg caagtgaagc ggacttaaat aaagcatctt
      421 tagcgcaaat ggcgcaatca aatgatcaaa cattaaatca aaaaccaatt gaagctgggg
      481 cttataatta tacatttgac tatgaagggt ttacttatca ctttgaatca gatggtacac
      541 actttgcttg gaattaccat gcaacaggtg ctaatggagc aaacatgagt gcacaagcac
      601 ctgcaactaa taatgttgaa ccatcagctg ttcaagctaa tcaagtacaa tcacaagaag
      661 ttgaagcacc acaaaatgct caaactcaac aaccacaagc atcaacatca aacaattcac
      721 aagttactgc aacaccaact gaatcaaaag catcagaagg ttcatcagta aatgtgaatg
      781 ctcatctaaa acaaattgct caacgtgaat caggtggcaa tattcatgct gtaaatccaa
      841 catcaggtgc agctggtaag tatcaattct tacaatcaac ttgggattca gtagcacctg
      901 ctaaatataa aggtgtatca ccagcaaatg ctcctgaaag tgttcaagat gccgcagcag
      961 taaaattata taacactggt ggcgctggac attgggttac tgcataagcc atttatgcat
     1021 agctaatcaa tagttatata agtaactttt agatcggaat atatcgggga attctcgata
     1081 tgttccggtc ttttttattt cgcaaatata cgtcattata gaattattat ttaaagataa
     1141 taattcaact gagcaacaat aattgaaaat gttgaaaccg tttgctaaga ttaatttgca
     1201 attgaatact aaggaactac taggggagcc taatgatatg gctgagatga attgttcaga
     1261 cccttatgac ctgatttggt tagtaccaac gtaggaaagt agttattgaa gtgatggggc
     1321 tagtatagca acattgccat caaactactt tcatgttgga agtagttttt ttatttaggg
     1381 aggatttttt acaatggaat tttcacaaaa attgtaccaa gctgcaaagc caatcattaa
     1441 tgatatttat gaggatgact ttattcagaa gatgttatcc ggagatatag gagctgacgc
     1501 attacgccat tacttaaaag ctgatgctgc gtatttaaaa gaatttacaa acttatatgc
     1561 tttgttaatt ccaaaaatga atagcatgaa tgacgtaaaa tttttagtag agcaaataga
     1621 atttatggtt gaaggtgaag tacttgcgca tgatatttta gcgcaaattg ttggtgaatc
     1681 atacgaagaa atcattaaaa caaaagtttg gcctccaagt ggcgaccatt acataaaaca
     1741 tatgtatttc caagcgcata gtcgtgaaaa tgctatttat acaattgctg caatggcacc
     1801 ttgtccatat atttatgcag aattagctaa gcgctctcaa agtgatcata agcttaatag
     1861 agaaaaagat acggcaaaat ggtttgattt ttatagtact gaaatggatg acataattaa
     1921 cgtatttgag gcattaatga ataaattagc cgaatcaatg tctgacaagg aattggaaca
     1981 agtaaagcaa gtatttttag aaagttgcat acatgagcgt cgatttttca atatggctat
     2041 gacactagaa caatgggaat ttggaggaaa agtaaatgat taaacctaaa atagcattaa
     2101 ccattgcagg tactgatcca acaggtggtg ccggcgtaat ggctgattta aaatcatttc
     2161 attcatgtgg tgtatatggt atgggcgtcg ttacaagtat tgttgctcaa aatacattgg
     2221 gcgtacaaca tattcataat ttaaatcatc aatgggtaga tgaacaactt gatagtgtct
     2281 tcaatgatac cttacctcat gctattaaaa cggggatgat tgctacagca gatactatgg
     2341 aaacgattcg tcattattta atgcaacatg aatctattcc atatgtaatt gatcctgtta
     2401 tgttggcgaa aagcggtgat tcattaatgg ataatgacac aaagcaaaac ttgcagcata
     2461 cgttattgcc attagctgac gtagtaacac cgaatttacc agaagctgaa gaaataacgg
     2521 gactaaccat tgatagtgaa gaaaaaatta tgcaggctgg ccgcatcttt attaatgaga
     2581 ttggtagtaa aggtatcatc attaaaggcg gtcattcaaa tgatactgat atagcaaaag
     2641 attatttatt tactaacgaa ggtgttcaaa catttgaaaa tgaacgattt aaaacaaaac
     2701 atacgcatgg aacagggtgt acattttcag cagttataac ggcagaactt gcaaaaggta
     2761 gaccattatt tgaggctgta cacaaggcta aaaagtttat ttcaatgagt atacaatata
     2821 cacctgaaat cggccgtggt agaggaccag tgaatcattt tgcatattta aagaaagagg
     2881 gattagacga tgaattatct aaataaaata cgtattgaaa acccattaac aatttgttat
     2941 acaaacgatg tagttaaaaa ttttacagcg aatggtttat taagtattgg tgcgagccct
     3001 gccatgagtg aagctcccga agaagctgaa gaattttaca aagttgcaca agcgctatta
     3061 atcaatatcg gtactttaac agcacaaaat gaacaagata ttattgcgat tgctcaaacg
     3121 gcaaatgagg caggcttacc tattgtattt gaccctgtag ctgttggtgc ttctacatat
     3181 cgaaagcaat tttgtaaatt attattgaaa tcagcgaaag tatcagtaat taaaggcaat
     3241 gcatctgaaa tattagcgtt gattgatgat acagcaacta tgaaaggtac agatagtgat
     3301 gctaatcttg atgcggttgc aatagcgaaa aaggcttacg caatatataa aactgcaata
     3361 gtaatcacag gtaaagagga cgttattgtt caagataata aagccatcgt attagctaat
     3421 ggatctccat tattagcacg agtaactgga gctggttgtt tattaggagg cgttattgct
     3481 ggatttttat ttagagaaac agaaccagac atagaagcgt taattgaagc ggtaagcgta
     3541 tttaatattg ctgctgaggt agctgctgaa aatgaaaatt gtggtggtcc tggtacgttt
     3601 tcaccattgt tgcttgatac gttatatcat ttaaatgaaa caacctatca acaacggatt
     3661 cgtattcaag aggtggaata atatgtttaa ccaatcgtat ctaaatgtgt attttatttg
     3721 tgggacctcc gatgttccga gtcatcgaac tattcatgaa gtattagaag cagcattaaa
     3781 ggcagggatt acacttttcc aatttcgaga aaagggtgaa tcagctttaa aaggaaacga
     3841 taaacttgta ttagctaaag aattacagca tctctgtcat caatatgatg tgccttttat
     3901 cgttaatgat gatgtatcct tggcaaaaga aattaacgcg gatggtatac atgtcggtca
     3961 agatgatgcg aaagtcaaag agatagcaca atatttcact gataaaatta ttggacttag
     4021 tattagtgat ttagatgagt atgcaaaatc tgatttaact catgttgatt atataggtgt
     4081 tggaccaatc tatcctacgc cgtcgaagca tgacgcgcat ataccagtag gtccagaaat
     4141 gattgcaacg tttaaagaaa tgaatccgca actaccgatt gtagctattg gcggtatcaa
     4201 tactaataat gtggcgccga ttgtagaagc aggtgccaat ggaatttcag tcatttcagc
     4261 catttctaaa agtgaaaata ttgaaaagac tgttaatcga ttcaaagatt tttttaataa
     4321 ttagttaaat ttttccactt attgtttcat acgtactcat taatgtgata gaataaatcc
     4381 tatgtgaata taatgataga ggtggaaaaa tgaagaaaaa agcgttacta ccattatttt
     4441 taggtattat ggtctttttg gctggttgtg actattctaa acctgaaaaa cgtagtgggt
     4501 ttttctacaa tacattcgta gatccaatga aaaatgtatt ggattggttg ggaaataact
     4561 tattaaacga caattatggt ttagctatta ttatccttgt attggtaatt cgtattattt
     4621 tattaccatt catgttgtca aactataaaa atagtcatat gatgcgtcaa aaaatgaaag
     4681 ttgcaaagcc agaagttgaa aaaattcaag aaaaagtgaa acgtgcgcgt acacaagaag
     4741 aaaaaatggc tgcaaaccaa gaattaatgc aagtatataa aaagtatgac atgaacccga
     4801 ttaagagtat gttgggttgt ttaccaatgc taatccaatt gccaatcatc atgggattat
     4861 actttgtact taaagaccaa cttgtagatg gtttgtttaa atatccacac ttcttatggt
     4921 tcgatttagg acgtcctgat atttggatta caattattgc cggtgtttta tactttatcc
     4981 aagcatatgt atcaagtaaa acgatgccag acgaacaacg gcaaatgggt tacatgatga
     5041 tggtcatttc accaattatg attatctgga tttcattaag ctcagcatca gcacttggtt
     5101 tgtactggtc tgtcagtgcg gcgttccttg tagttcaaac acactttgcg aacatttatt
     5161 atgaaaaagt cgctaaaaaa gaagtacaac ctttcattga agcgtatgaa agagagcaca
     5221 acggcggcag caataaaaaa ggcaaaaaca cacaagtagt gtctaaaaag aaaaagaaat
     5281 aaccttagca agtccaaata atgtgcaccc taaaagttga agataatttt aactttatag
     5341 ggcgcacatt atttttcgct tcaatagcga aacttagtgt cacattatac aatatgtgat
     5401 aaatagaaac agcagtaaga tgatttcaaa tgtgaaagtc atcctactgc tgtttttatt
     5461 tgaaataata ttaacgagat ttcagtatgt aaaaagtggt taataacaga gttgtcattc
     5521 ttcatcgcaa tgtcatcttg acacaatgat tcatcagtgt agcagtatat gtcgcaaatt
     5581 tccacagtaa ttcactaata catgtaacag tgtgaaatac taacatcaca acggcataat
     5641 acacattgca acgtcccaac aacatatcat agaatagtaa cggcatggca tcaacaccaa
     5701 accgggctat aataccataa caccgcaacg ccacatcata gcactacatc aagtcgcatc
     5761 gtagcaccag cacatcaaag taacatggca tcaacatcaa accggtctat aacaccataa
     5821 cagcgcaacg ccacatcata gcactacatc aagtcgcatc gtggtaccag cacatcaaag
     5881 taacatggca tcaacgccaa accggtctat aacaccataa caccgcaatg ccacatcata
     5941 gcactacatc aagacgcatc gtggtaccag cacatcaaag tagcatggca tcgacgccaa
     6001 accggtctat aacaccataa caccgcaatg ccacatcata gcactacatc aagacgcatc
     6061 gtggtaccag cacatcaaag taacatgaca tgacaccaag ctcccacaac aacacatcaa
     6121 agtaacatgg catgacacca agcttccaca acaacacgcc attacatcgc atcatcatac
     6181 tacaacttca atgtctagtc gtgataattc cattccgtaa aaaactgttt caagtacatc
     6241 atcataaagt cgtgacgttc tttagcaatc atcttcgccg tatctgtatg cattaaagat
     6301 tctaacttaa gtaatttttc aaagaaatgt ttaattgcag atggtggcaa ctgttcaact
     6361 aaatcatcat taatcttatc tagtgacata tgttctgtcc acattggttc accaaagtgt
     6421 cctgcaaatt gaaatgttcg tgcaacacct atagcgccta tagcatcaag acgatctgca
     6481 tccctgacaa tttgaccttc taaagataaa gtgacatgat cgtttttgcc attgcgatag
     6541 ctcatattat taataataaa taaaatatgc tcttgatctt cggttgatag tgataaagaa
     6601 gataaaaatg acttcaattc aacatattgt ttgttagcat ctacaacttt ttcgtcaacg
     6661 gtatcatgaa gcaaacatgc gagttcaatg actaaagtat cattaacacc ttcattttca
     6721 gcgattgatt tagctaaagc agtgacacga tatacatgcg caatatcatg gcctgtataa
     6781 tcattttgat gaatagattc catatatttt ttggcagcac taatgacgtc tgattgttgc
     6841 attaaagtta cactcctcat atttctattt tataagatag gtgacaataa ttgtgaaata
     6901 ccttctttaa atttaatcca aagacttcgc ttagcataac gtgcttttgt taattcagaa
     6961 gatactgcta aatcatctat aaaagcttgt tttaattttt tggcaatttg ttggtcataa
     7021 ataaaagcgt tgacttcgaa attcaatgtg aaactgcgat ggtccatatt agctgttccc
     7081 acacttgcaa tttcatcatc tataacaagt gtttttgagt gtaaaaagcc attgtcgtag
     7141 tgaaatactt taacaccggc atctaataag gatgctgcat tttttaaagt agcccaaaaa
     7201 acaaacggat ggtcaggttt attaggaatc atgatattga catcaacacc acctaatgcc
     7261 gcaattttaa tagaatctaa aaaggcttga tcaggtatga aatagggaga ttgaatataa
     7321 atcgattttt tagcagatga aatcattttc aaatagccgt atttaatctg ttcccattct
     7381 tcgtcaggac cactagaagc tatttgaacg ccaattgttc caccagaatt tacatctggg
     7441 aaataacgat catcatagga gatgtggtca cgtgtggctt gtgaattcca atctagaata
     7501 aatcgtaatt gcaatgcatt cactgcatcc ccgacaattc gtaaatgcgt atctcgccaa
     7561 tagccgaatt tttttgattt acctaagtac tcatcaccaa cattaaaacc accaacatat
     7621 ccaatttgcc catctattac aacaattttt cgatggtttc gattgttcat acgcaagtta
     7681 attaaaggta attttgatgg gaaaaatgct tcagcatgtc cacttttatt gcgaaacggg
     7741 cgtaagcctt ttttacgcag tccacgagaa cccatgtcat cataaagaat tttaacttct
     7801 acaccttgtt ccgctttttt accaagttca tttaaaatgg tacgacctaa ttcatcattt
     7861 tgaataatat agtactgaaa atgaatataa tcagtagcat tacggatgtc ttgtattagg
     7921 tcatcaaatt tttcttggcc gtctgtgtat atttttaaat cgttgtctgt tgttaaaaat
     7981 gctgcgttat tatataacaa catttgaatc atttctttaa attttacaat ttgataattg
     8041 gaatttgaaa agttttcatt ttttaaagca gctaattgct catcaacgat taactctaat
     8101 ccttttttat cttccttatc aattttgaaa atttggtcac gttgaatttg tcgtcctaat
     8161 agtaagtata aaatgaagcc gaataaaggc aagaaaacta agactagtaa ccaagcccag
     8221 atagaattgg cagaacgtct ttccatgaaa ataatggtaa aggcgaataa taagtttaaa
     8281 ataaatgcac caataaatat tgaatttaaa ataatattag aatgcttgag tgcaatggat
     8341 aataactcta tcatttgaaa cctcccatcg aaaatcttaa acgagtagta tatcttatta
     8401 attgtatcat gaagcaattt aaatatcatg acaatttagt ttgaaaaaca cactttgtgt
     8461 tgacgttgtg ttttatggga tatagtatag taaggaatgt aaatgaagga gtgaatgcta
     8521 tgactgaaca agataatgca catcattctg aacaaataaa aacgaatctt aaatcacgtt
     8581 taaatcgaat tgaaggacaa gtgagagcga ttaatcgcat gattgaagaa gatgtctatt
     8641 gtgatgatgt ccttacgcaa ataagagcga cacgttcggc gttaaacagt gttgcgataa
     8701 agttattaga acaacatatg aaaagttgta ttatgaataa agttaatcaa ggtgctcagg
     8761 aagaggcaat ggaagagtta ttagtgactt ttcaaaaatt gattaaagac taaaggagtt
     8821 taagatgata catcaaaata cgatttacac agcgggaatt gaaacagaag aacaagtaag
     8881 tcaattgaca gaacgcattt caaatatgat aggtgttcat caagtgaata ttaatataat
     8941 agatggtcaa gtaactgtat cgtatgagac accagcaaat ttgaatagta ttgaaaaaga
     9001 aatctatgat gaaggataca aaattgtatt ttagggtata atgtagagtg cgctatagat
     9061 tttaattttg aaaataaatt aaaaattttg taaatgatgt agtaaaggta tgtcgaatag
     9121 ggtaagataa tattgaaagc gatccaaacg aggtgataat aatgaaacgt cctgaaaaga
     9181 ttcaaaatgt agtcaaacta ttgtcatcat taggtgtgaa tattaaaaaa actaaatctc
     9241 gtttagacat tattaatact ttgccagcat ctaataaagt aagtcacgaa ttaaaataat
     9301 tgactgtgat tgtgaatcac acaccgttaa agctatcgtc ggactattcg atgatagctt
     9361 ttttgactgg atttactaaa atgaaagcaa gtactatata acaaaatccc tctttagagt
     9421 gtacatttta caatgtgtac ttcaaagagg gattgttatt atcgagtaat atctatatat
     9481 gacattttaa atcgacgacg taaatagact atataacgtc atcttgaaaa gttatgatat
     9541 ttgaaactca aaatagttta aattaattac tttttggatg gtataaatcg acatatcgtt
     9601 ttggttcatg ataataaatt gataagacta tacctattcc agtcatcata ctccatagcg
     9661 cactaccacc ataactaata aatggtaatg gaataccagt gattggtaac aactgaattg
     9721 tcataccaat attttgtaaa atatggaaca caagtaaagt gacgaaacca acgataaaga
     9781 ttttgttaaa ttgatcttca attttcgcag ctaatcttat tagatggaag attaaaaata
     9841 aaaatattaa gatcaatatg acagaaccga taaagccaag ttcctctcca atcactgaaa
     9901 agataaagtc agtatgattt tcaggtatat aaacttcacc gtgattgtat cctttaccta
     9961 gtaactgtcc agaaccgata gctttaagtg attcagttaa atgatagcca tcaccactac
    10021 tatatgtata ggggtcaagc catgaattga ttcgtcccat ttgatacagt tggacaccta
    10081 ataaattttc aattaatgcg ggtgcatata gaatacctaa aatgactgtc attgcaccaa
    10141 caatacctgt aataaagata ggtgctaaga tacgccatgt tataccactt actaacatca
    10201 cacctgcaat aatagcagct aatactaatg tagttcctag gtcattttgc agtaatatta
    10261 aaatacttgg tactaacgag acaccaataa ttttgaaaaa taataacaaa tcactttgga
    10321 atgatttatt gaatgtgaat tgattatgtc tagaaacgac acgcgctaat gctaaaatta
    10381 aaataatttt catgaattca gatggctgaa tactgatagg gccaaacgtg taccaacttt
    10441 tggcaccatt gataataggt gtaataggtg actcaggaat aacgagcaag cctattaata
    10501 atagacagat taagaaatac aataaatatg tataatgttt aatcttttta ggtgaaataa
    10561 acatgatgat acctgcaaaa attgcaccta aaatgtaata aaaaatttgt ctgataccga
    10621 aattagcact gtattgacca ccgcccattg ccgagttaat aagcagaaca ctgaaaattg
    10681 ctaaaacagc tatagtggct actaataccc agtctacttt gcgaagccaa tgcttatccg
    10741 gctgttgacg agatgaataa ttcattgcaa actcctttta tactcactaa tgtttatatc
    10801 aattttacat gactttttaa aaattagcta gaatatcaca gtgatatcag ctatagattt
    10861 caatttgaat taggaataaa atagaaggga atattgttct gattataaat gaatcaacat
    10921 agatacagac acataagtcc tcgtttttaa aatgcaaaat agcattaaaa tgtgatacta
    10981 ttaagattca aagatgcgaa taaatcaatt aacaatagga ctaaatcaat attaatttat
    11041 attaaggtag caaaccctga tatatcattg gaggaaaacg aaatgacaaa agaaaatatt
    11101 tgtatcgttt ttggagggaa aagtgcagaa cacgaagtat cgattctgac agcacaaaat
    11161 gtattaaatg caatagataa agacaaatat catgttgata tcatttatat taccaatgat
    11221 ggtgattgga gaaagcaaaa taatattaca gctgaaatta aatctactga tgagcttcat
    11281 ttagaaaatg gagaggcgct tgagatttca cagctattga aagaaagtag ttcaggacaa
    11341 ccatacgatg cagtattccc attattacat ggtcctaatg gtgaagatgg cacgattcaa
    11401 gggctttttg aagttttgga tgtaccatat gtaggaaatg gtgtattgtc agctgcaagt
    11461 tctatggaca aacttgtaat gaaacaatta tttgaacatc gagggttacc acagttacct
    11521 tatattagtt tcttacgttc tgaatatgaa aaatatgaac ataacatttt aaaattagta
    11581 aatgataaat taaattaccc agtctttgtt aaacctgcta acttagggtc aagtgtaggt
    11641 atcagtaaat gtaataatga agcggaactt aaagaaggta ttaaagaagc attccaattt
    11701 gaccgtaagc ttgttataga acaaggcgtt aacgcacgtg aaattgaagt agcagtttta
    11761 ggaaatgact atcctgaagc gacatggcca ggtgaagtcg taaaagatgt cgcgttttac
    11821 gattacaaat caaaatataa agatggtaag gttcaattac aaattccagc tgacttagac
    11881 gaagatgttc aattaacgct tagaaatatg gcattagagg cattcaaagc gacagattgt
    11941 tctggtttag tccgtgctga tttctttgta acagaagaca accaaatata tattaatgaa
    12001 acaaatgcaa tgcctggatt tacggctttc agtatgtatc caaagttatg ggaaaatatg
    12061 ggcttatctt atccagaatt gattacaaaa cttatcgagc ttgctaaaga acgtcaccag
    12121 gataaacaga aaaataaata caaaattgac taactgaggt tgttattatg attaatgtta
    12181 cattaaagca aattcaatca tggattcctt gtgaaattga agatcaattt ttaaatcaag
    12241 agataaatgg agtcacaatt gattcacgag caatttctaa aaatatgtta tttataccat
    12301 ttaaaggtga aaatgttgac ggtcatcgct ttgtctctaa agcattacaa gatggtgctg
    12361 gggctgcttt ttatcaaaga gggacaccta tagatgaaaa tgtaagcggg cctattatat
    12421 gggttgaaga cacattaacg gcattacaac aattggcaca agcttacttg agacatgtaa
    12481 accctaaagt aattgccgtc acagggtcta atggtaaaac aacgactaaa gatatgattg
    12541 aaagtgtatt gcataccgaa tttaaagtta agaaaacgca aggtaattac aataatgaaa
    12601 ttggtttacc tttaactatt ttggaattag ataatgatac tgaaatatca atattggaga
    12661 tggggatgtc aggtttccat gaaattgaat ttctgtcaaa cctcgctcaa ccagatattg
    12721 cagttataac taatattggt gagtcacata tgcaagattt aggttcgcgc gaggggattg
    12781 ctaaagctaa atctgaaatt acaataggtc taaaagataa tggtacgttt atatatgatg
    12841 gcgatgaacc attattgaaa ccacatgtta aagaagttga aaatgcaaaa tgtattagta
    12901 ttggtgttgc tactgataat gcattagttt gttctgttga tgatagagat actacaggta
    12961 tttcatttac gattaataat aaagaacatt acgatctgcc aatattagga aagcataata
    13021 tgaaaaatgc gacgattgcc attgcggttg gtcatgaatt aggtttgaca tataacacaa
    13081 tctatcaaaa tttaaaaaat gtcagcttaa ctggtatgcg tatggagcaa catacattag
    13141 aaaatgatat tactgtgata aatgatgcct ataatgcaag tcctacaagt atgagagcag
    13201 ctattgatac actgggtact ttgacagggc gtcgcattct aattttagga gatgttttag
    13261 aattaggtga aaatagcaaa gaaatgcata tcggtgtagg taattattta gaagaaaagc
    13321 atatagatgt gttgtatacg tttggtaatg aagcgaagta tatttatgat tcgggccagc
    13381 aacatgtcga aaaagcacaa cacttcaatt ctaaagacga tatgatagaa gttttaacaa
    13441 gcgatttaaa agcgcatgac cgtgtattag ttaaaggatc acgtggtatg aagttagaag
    13501 aagttgtaaa tgctttaatt tcatagagat tagtcgagcg accttttact tataaaaatg
    13561 atttgaatta atactaaaag attacaaaga agaggtggtt ttgtgtgtaa atacaaaatt
    13621 gcctttttct ttttatgtta aatctataaa tttgaaacta aatcaaggtt aattctatgt
    13681 acacacttta tataggaa
//
LOCUS       DAHHSC010000055        12859 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.055, whole genome shotgun sequence.
ACCESSION   DAHHSC010000055 DAHHSC010000000
VERSION     DAHHSC010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 12859)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12859)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12859
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.055"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            146..439
                     /locus_tag="NBI57_001214"
                     /pseudo
     CDS             146..439
                     /locus_tag="NBI57_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000277741.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(575..1477)
                     /gene="cvfB"
                     /locus_tag="NBI57_001215"
     CDS             complement(575..1477)
                     /gene="cvfB"
                     /locus_tag="NBI57_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding virulence regulatory protein CvfB"
                     /protein_id="HCD1822171.1"
                     /translation="MALDKDIVGSIEFLEVVGLQGSTYLLKGPNGENVKLNQSEMNDD
                     DELEVGEEYSFFIYPNRSGELFATQNMPDITKDKYDFAKVLKTDRDGARIDVGLPREV
                     LVPWEDLPKVKSLWPQPGDHLLVTLRIDRENHMYGRLASESVVENMFTPVHDDNLKNE
                     VIEAKPYRVLRIGSFLLSESGYKIFVHESERKAEPRLGESVQVRIIGHNDKGELNGSF
                     LPLAHERLDDDGQVIFDLLVEYDGELPFWDKSSPEAIKEVFNMSKGSFKRAIGHLYKQ
                     KIINIETGKITLTKKGWSRIDSKE"
     gene            1624..3225
                     /locus_tag="NBI57_001216"
     CDS             1624..3225
                     /locus_tag="NBI57_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCD1822172.1"
                     /translation="MLQVTDVSLRFGDRKLFEDVNIKFTEGNCYGLIGANGAGKSTFL
                     KILSGELDSQTGHVSLGKNERLAVLKQDHYAYEDERVLDVVIKGHERLYEVMKEKDEI
                     YMKPDFSDEDGIRAAELEGEFAEMNGWNAEADAANLLSGLGIDPTLHDKKMAELENNQ
                     KIKVLLAQSLFGEPDVLLLDEPTNGLDIPAISWLEDFLINFDNTVIVVSHDRHFLNNV
                     CTHIADLDFGKIKVYVGNYDFWYQSSQLAQKMAQEQNKKKEEKMKELQDFIARFSANA
                     SKSKQATSRKKQLEKIELDDIQPSSRRYPFVKFTPEREIGNDLLIVQNLSKTIDGEKV
                     LDNVSFTMNPNDKAILIGDSEIAKTTLLKILAGEMEPDEGSFKWGVTTSLSYFPKDNS
                     EFFEGVNMNLVDWLRQYAPEDEQTETFLRGFLGRMLFSGEEVKKKASVLSGGEKVRCM
                     LSKMMLSSANVLLLDEPTNHLDLESITAVNDGLKSFKGSIIFTSYDFEFINTIANRVI
                     DLNKQGGVSKEIPYEEYLQEIGVLK"
     gene            4287..5492
                     /locus_tag="NBI57_001217"
     CDS             4287..5492
                     /locus_tag="NBI57_001217"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HCD1822173.1"
                     /translation="MVTRSVLKFGGSSVSDFTKIKRIAEMLKERVNQDEQLIVVVSAM
                     GNTTDQLMTNVSTLTKAPKQQELALLLTTGEQQTVSYLSMVLNDIGVNAKAMTGYQAG
                     IKTIGHHLKSKIAQINPQTFEQAFQENDILVVAGFQGINEHQELTTLGRGGSDTTAVA
                     LAVSNQIPCEIYTDVDGVYATDPRLLPKAKRLDIVSYEEMMEMSALGAGVLETRSVEL
                     AKNYNIPLYLGKTLSNVKGTWIMSNEEILEKKAVTGVALDKHMMHVTISYPLPDNQLL
                     TQLFTELEEGAVNVDMISQIVNLDGLQLSFTIKDSDFHQISMILETLKNQYEALAYKI
                     NEHYVKISLIGSGMRDMSGVASKAFLTLIENNIPFYQTTTSEISISYVIDDFNGQQAV
                     EKLYDAFNI"
     gene            5556..6545
                     /locus_tag="NBI57_001218"
     CDS             5556..6545
                     /locus_tag="NBI57_001218"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HCD1822174.1"
                     /translation="MTKLAVVGATGLVGTKMLETLNRKNIPFDELVLFSSARSAGQEV
                     EFQEKTYTVQELTDARASEHFDYVLMSAGGGTSEHFAPLFEKAGAIVIDNSSQWRMAE
                     DIDLIVPEVNEPTFTRGIIANPNCSTIQSVVPLKVLQDAYGLKRVAYTTYQAVSGSGM
                     KGKKDLAEGVNGKAPEAYPHPIYNNVLPHIDVFLENGYTKEEQKMIDETRKILNAPDL
                     KVTATCARVPVQDSHSVEIDVTLDKETTAEDIKALFDQDDRVVLVDNPENNEYPMAIN
                     STNKDEVFVGRIRRDDSLENTFHVWCTSDNLLKGAALNAVQVLEQVMRLKGAN"
     gene            6547..7434
                     /locus_tag="NBI57_001219"
     CDS             6547..7434
                     /locus_tag="NBI57_001219"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HCD1822175.1"
                     /translation="MTHLFEGVGVALTTPFTNNKVNLEALKAHVNFLLENNAQAIIVN
                     GTTAESPTLTTDEKERILKTVIDLVDKRVPVIAGTGTNDTEKSIQASFQAKALGADAI
                     MLITPYYNKTNQRGLVKHFEAITDAVKLPVVLYNVPSRTNMTIEPETVEILSQHPYIV
                     ALKDATNDFEYLEEVKKRIDTNSFALYSGNDDNVVEYYQRGGQGVISVIANVIPKEFQ
                     ALYDAQQSGLDIQDQFKPIGTLLSALSVDINPIPIKALTSYLEFGNYELRLPLVSLED
                     TDTKVLREAYDTFKAGENE"
     gene            7431..8153
                     /locus_tag="NBI57_001220"
     CDS             7431..8153
                     /locus_tag="NBI57_001220"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000698231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HCD1822176.1"
                     /translation="MKILLIGYGAMNQRVARLAEEKGHEIVGVIENTPKATTPYQQYQ
                     HIADVKDADVAIDFSNPNLLFPLLDEEFHLPLVVATTGEKEKLLNKLDELSQNMPVFF
                     SANMSYGVHALTKILAAAVPLLDDFDIELTEAHHNKKVDAPSGTLEKLYDVIVSLKEN
                     VTPVYDRHELNEKRQPQDIGIHSIRGGTIVGEHEVLFAGTDETIQITHRAQSKDIFAN
                     GAIQAAERLVNKPNGFYTFDNL"
     gene            8180..8899
                     /gene="dapD"
                     /locus_tag="NBI57_001221"
     CDS             8180..8899
                     /gene="dapD"
                     /locus_tag="NBI57_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HCD1822177.1"
                     /translation="MVQHLTAEEIIQYISDAKKSTPIKVYLNGNFEGITYPESFKVFG
                     SEQSKVIFCEADDWKPFYEAYGSQFEDIEIEMDRRNSAIPLKDLTNTNARIEPGAFIR
                     EQAIIEDGAVVMMGATINIGAVVGEGTMIDMNATLGGRATTGKNVHVGAGAVLAGVIE
                     PPSASPVIIEDDVLIGANAVILEGVRVGKGAIVAAGAIVTQDVPAGAVVAGTPAKVIK
                     QASEVQDTKKEIVAALRKLND"
     gene            9042..10193
                     /locus_tag="NBI57_001222"
     CDS             9042..10193
                     /locus_tag="NBI57_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HCD1822178.1"
                     /translation="MNELEFVTKHRRHLHQHPELSLHEFETTAYIKAFLDSLNIKYDC
                     PLETGVIAYLEGNGSHTIAYRADIDALPILEENDVPYRSQSDHVMHACGHDGHTTALM
                     LFVQRCKDMQDAGQLPQNVVFIFQPAEETGGGANRLIKAGAFDKYPIEAVFGIHVNPF
                     ADEGIAVIRDEEITASATEYRFFLTGLSSHVADKEQAHSCGEALQHVLTQISQIQQFH
                     LNGLKRNIVHIGHFKAGEAINTVPSNGYLEGTIRTYDIDDLTIVKNQMHKIAESVKLL
                     FNVECEVKFAEGYPPTINSPKLRTQIEDALIKADLNVYDKPTPFLFGEDFSFYGQQLA
                     PAYFVFIGTRNEDKGFVTGLHTSHLNFDEKALINVVNFYENLLNNYKEV"
     gene            10198..11283
                     /locus_tag="NBI57_001223"
     CDS             10198..11283
                     /locus_tag="NBI57_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HCD1822179.1"
                     /translation="MTATWSVNKKIFLQNAITVKNNQPLMAVVKNNAYHYDLEFAVTQ
                     FIHAGIDTFSTTSLREAIQIRQLAPDATIFLMNAVYEFDLVREHQIHMTLPSLTYYYN
                     HKNDLAGIHVHLEFENLLHRSGFKDLNEIKEVLKDHHHNQNAKMIISGLWTHFGYADE
                     FDVSDYNVERSQWMEIVEALLSEGYQFDLIHAQNSASFYREGQILLPHHTHARVGIAL
                     YGSRPYSSLNQHDIVQSLTVKAHVIQVREVQAGDYCGYSFAFEVTKNNTKLAVVDIGY
                     GDGILRTRAKHEALINGKRYPIRALMMSHMFVEVDGNVHAQDEVILYNNDIRIDEYTF
                     KGVGANSEQLSAMNHDSLKKEYISNDC"
     gene            11273..12538
                     /gene="lysA"
                     /locus_tag="NBI57_001224"
     CDS             11273..12538
                     /gene="lysA"
                     /locus_tag="NBI57_001224"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HCD1822180.1"
                     /translation="MTVKYNQNGELTMDGISLKTIAQSFGTPTIVYDELQIREQMRRY
                     HRAFKDSGLKYNISYASKAFTCIQMVKLVAEEDLQLDVVSEGELYTALEAGFEPSRIH
                     FHGNNKTKHEIRYALENNIGYFVIDSLEEIELIDRYANDTVQVVLRVNPGVEAHTHEF
                     IQTGQEDSKFGLSIQYGLAKKAIDKVQQSKHLKLKGVHCHIGSQIEGTEAFIETAKIV
                     LRWLKEQGIQVELLNLGGGFGIKYVEGDESFPIESGIKDITDAIKSEIKVLGIDAPEI
                     GIEPGRSIVGEAGVTLYEVGTIKEIPEINKYVSIDGGMSDHIRTALYDAKYQALLVNR
                     NEEADDSVTIAGKLCESGDIIIKDAKLPSSVKRGDYLAILSTGAYHYSMASNYNQMQK
                     PSVFFLKDGKAREVIKRQSLRQLIINDTK"
     gene            complement(12778..>12859)
                     /locus_tag="NBI57_001225"
     CDS             complement(12778..>12859)
                     /locus_tag="NBI57_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MSA protein"
                     /protein_id="HCD1822181.1"
                     /translation="ILFYTLYLLLILIVLYYLKKQVKHKI"
BASE COUNT         4455 a         2050 c         2352 g         4002 t
ORIGIN      
        1 ggtgccgtct cagccacccc aaccggcaca ttgttgtaag ctgactatat gtcagcttct
       61 gtgttggggc ccctgtcttc gactggcact gctccctcag gagtctcgcc attaatacta
      121 cgtattaaca tgtaatttta ctttgaaata cattaaagcc caaattaatc aaaagctttc
      181 tgaaccagaa acgaaaaaaa tctatagtca tagaaaaatt tatgtagagc ctgtttttgg
      241 atttatgaag gttattttgg gtttcactcg aatgtcagtt cgaggaataa ataaagttaa
      301 acgagagcta ggttttgtat taatggcact taatataagg aaaatagcag ctcaacgagc
      361 tgtacattat aaaatacata tcaaaaaagc taatttccat caaataatta atagaaatca
      421 gcttttttac attgcctaag aacttaatgt cccagcctat tcgtttgaat tatttaattt
      481 tttactttga aaggaatgtt taaaaaacta cttcatctgt ccgacttaat attgaatata
      541 aaaaacacgc atcctacgac acgtgtaaaa atgattattc ttttgagtca attcgactcc
      601 aacctttttt agttaaagtg attttacctg tttctatatt aataatcttc tgtttatata
      661 agtgaccgat tgcacgtttg aatgaacctt tactcatatt gaatacttct ttaatcgctt
      721 cagggcttga tttgtcccag aatggtaatt caccatcata ttcaactagt aaatcaaaga
      781 tgacttggcc gtcatcgtct aaacgttcat gtgcaagtgg taaaaatgaa ccatttaact
      841 cacctttatc attatgcccg ataattctaa cttgaacaga ttcacctaat cttggttcag
      901 ctttacgttc tgattcatgt acgaaaattt tgtaacctga ttcgcttaat aagaagctac
      961 caattcgtaa tacgcggtaa ggtttggctt caatgacttc gttttttaaa ttatcatcgt
     1021 gtacaggtgt aaacatattt tctacaacag attcactcgc taaacgtcca tacatatgat
     1081 tctcacggtc aattcgtaat gtaactagca aatgatcacc aggttgtggc catagtgatt
     1141 tcacttttgg taaatcttcc catggtacta acacttcacg gggtaaacca acatctatac
     1201 gtgccccatc gcgatccgtt ttaagtactt tagcaaaatc atatttatct ttcgtaatat
     1261 caggcatatt ttgagttgca aataattcac ctgaacggtt tggataaatg aagaaactat
     1321 attcttcacc tacttctaat tcatcatcat cgttcatttc tgattggttt aactttacgt
     1381 tttcaccgtt tggtcctttt aaaaggtaag ttgagccttg taaccctact acttcaagga
     1441 attctataga acctactata tctttgtcta atgccatgtt attctccttc gattcttgta
     1501 ttctttatta ttataacatg tgtgagtcat tctatcatta caacttatat gaggtcgaaa
     1561 ctgtgacgat aatatgatat aattcttccg ttagaatatc gaatgaaaaa aaggagaaaa
     1621 tgcatgttac aagtaactga tgtgagttta cgttttggag atcgtaaact atttgaagat
     1681 gtaaatatta aatttacaga aggtaattgt tatggattaa ttggtgcgaa tggtgcaggt
     1741 aaatcaacat tcttaaaaat attatctggt gaattagatt ctcaaacagg acatgtttca
     1801 ttaggtaaaa atgaacgtct agctgtttta aaacaggacc actatgctta tgaagatgaa
     1861 cgcgtgcttg atgttgtaat taaaggtcac gaacgtcttt atgaggttat gaaagaaaaa
     1921 gatgaaatct atatgaagcc agatttcagt gatgaagatg gtatccgtgc tgctgaactt
     1981 gaaggtgaat ttgcagaaat gaatggttgg aatgctgaag ctgatgctgc taacctttta
     2041 tctggtttag gtatcgatcc aactttacac gataaaaaaa tggctgaatt agaaaacaac
     2101 caaaaaatta aagtattatt agcgcaaagt ttattcggtg aaccagacgt actattactg
     2161 gatgagccta ctaacggtct cgatattcca gcaatcagtt ggttagaaga tttcttaatt
     2221 aactttgata atactgttat cgtagtatcg catgaccgtc atttcttaaa taatgtatgt
     2281 actcatatcg ctgatttaga cttcggtaaa attaaagttt atgttggtaa ctatgatttt
     2341 tggtatcaat ctagtcagtt agctcaaaag atggctcaag aacaaaacaa gaaaaaagaa
     2401 gaaaaaatga aagagttaca ggactttatt gcacgtttct cagctaacgc ttctaaatct
     2461 aaacaagcaa caagtcgtaa aaaacaactt gagaaaattg aattagatga tattcaacca
     2521 tcatcaagaa gatatccttt cgttaaattc acgcctgagc gtgagattgg taacgactta
     2581 ttaatcgttc aaaatctttc taaaacaatt gacggcgaaa aagtattaga taatgtatca
     2641 ttcacaatga atccaaatga taaagcgatt ttaattggag atagtgaaat tgcaaaaaca
     2701 acattactta aaatattagc tggcgaaatg gaaccagacg aaggttcatt taaatggggt
     2761 gttactacat cattaagtta cttccctaaa gataactcag agttctttga gggtgtaaat
     2821 atgaatctcg ttgattggtt aagacaatat gctcctgaag atgaacaaac agaaacattt
     2881 ttacgtggtt tcttaggtcg tatgttattt agtggtgaag aagttaagaa aaaagctagt
     2941 gtgctttcag gtggagaaaa agtacgttgt atgctaagta aaatgatgtt atcaagtgcg
     3001 aatgtacttt tacttgacga acctactaac cacttagact tagaaagtat tactgctgtc
     3061 aatgatggtc ttaaatcatt taaaggttct atcatcttta cttcttatga cttcgaattt
     3121 atcaacacga ttgcaaaccg tgttatcgat ttaaataaac aaggcggcgt ttcaaaagaa
     3181 attccatatg aagaatactt gcaagaaatc ggcgttttaa aataaacata atatgtagaa
     3241 tttcttttcg aaaatctctg tattgaggcc cgaccaactt gcacattatt gtaagttggc
     3301 ttactgtcac cttctatatt gggacttatt ccgcaacttg tattgtttgt agaaattagg
     3361 aatccaattt ctctatgtgt tgcaccgaca aggattgaaa aaagctagct acaaagtcat
     3421 tttcattcag tcaactactg tcaatataac attgcagggt ctagggcatt gattcatgcc
     3481 cgagactata ttcaatttaa tagacattaa ctggtcgtga ggctgggaca taaatcccta
     3541 aaaaaacagc agtaagataa ttttcaatta gaaaatatct tactgctgtt ctctatttat
     3601 acaatacttc gtattgaatg gcttcgcttt gcccgtctgg cacattactg taaaattcta
     3661 taaatagaat ttttgatgac gggtcccttc ctagggtgcc gtctcagcct ctttttacat
     3721 tgcctaagaa cttaatatcc cagcctcgtg tcctttttat tttatttttt agcacatcag
     3781 cacagtgaac accccttctc taaattacaa atacagttat caccgcatta aaagagtaca
     3841 tttatttaat gtgcatattg agtatgctat aagtattttg aattttattt ttcaaaaagt
     3901 ctcattatca ccacctttta aaatataagc aacgctataa cttgaatttt caggttattc
     3961 caaaaaatct aaattatcgg tttacattca tatttgtcat atgtataatg aacacatacc
     4021 aatttaataa tattttgatg aggcgcatca atcatgagta aagtttagat tactgtctgc
     4081 taacagctga atttgaaagg gtgcgatgcc gaagcgatta taatagcagt tataatttgt
     4141 tggacttttt ggttaagagc tgagagtttg tcattattta aaaataatgg agtgcatcac
     4201 ttgtatatag attaagagca agttcgcatt ccgaacttgt tcttttttta ttattgtgtg
     4261 cccttcctaa caattaggag gattatatgg taacaagaag tgtgttgaaa tttggcggat
     4321 catccgtcag tgattttaca aaaataaaaa ggatcgctga aatgttaaag gagcgagtca
     4381 atcaagatga acaattaatt gtcgttgtaa gtgctatggg taacacaaca gatcaattaa
     4441 tgacgaatgt atcgaccttg actaaagcac caaaacaaca agaactggca ttattattga
     4501 caaccggaga gcaacaaact gtatcttatt tatcaatggt attaaatgat atcggtgtga
     4561 atgccaaagc aatgactggc tatcaagcgg gtattaaaac cattggccat catttaaaaa
     4621 gtaaaattgc tcaaattaat cctcaaacat ttgaacaagc ctttcaagaa aacgatattt
     4681 tagtagttgc tggatttcaa ggcatcaatg aacatcagga attaacaact ttaggcagag
     4741 gcggttctga tacgaccgct gtggcacttg ctgttagtaa tcaaatacct tgtgaaattt
     4801 ataccgacgt tgatggtgtg tatgccactg acccaagact tttaccaaag gctaaacgac
     4861 tagacatcgt ctcatatgaa gaaatgatgg aaatgagcgc tttaggtgct ggtgtacttg
     4921 aaacaagaag tgttgaatta gctaaaaact ataatatccc tttatattta ggaaaaactt
     4981 tatcgaacgt gaaaggaaca tggattatgt caaatgaaga aatattagag aaaaaagcag
     5041 ttactggtgt ggctttggat aaacatatga tgcatgtaac aattagttat cccctacctg
     5101 acaatcagct acttacccaa ctatttacgg aacttgaaga aggtgctgta aatgttgata
     5161 tgatttcaca aatcgtcaac ttggatgggc tacaactatc cttcacgatt aaagatagtg
     5221 attttcatca aatttctatg attcttgaaa cattaaagaa tcaatatgaa gcattagctt
     5281 ataaaatcaa tgagcattat gtcaaaattt cattaattgg ctcaggcatg cgtgatatgt
     5341 caggtgtggc atcaaaagca tttttgacat taattgaaaa taatatacct ttctaccaaa
     5401 caacaacatc tgaaataagt atttcatacg tcattgatga ttttaatggg caacaagcgg
     5461 tagaaaaact atatgacgca tttaacattt aatggtaaaa tgattgttaa aatattctaa
     5521 aaattggaaa ttattataaa atggagtgac aagttatgac aaagttagca gttgtgggtg
     5581 caacaggatt agtaggcaca aaaatgttgg agacattaaa tcgtaaaaat attcctttcg
     5641 atgaattagt attattttca tcagcacgtt ctgcagggca agaagttgaa tttcaagaaa
     5701 aaacatatac agtacaagaa ttaactgatg ctcgtgctag tgaacatttc gattatgtat
     5761 taatgagtgc tggtggcggt acaagcgaac actttgcccc actctttgaa aaagctggtg
     5821 caatcgttat agacaattca agtcaatggc gtatggcaga agatattgat ttaatcgttc
     5881 cggaagtcaa tgaacctaca tttacaagag gtatcattgc caatccaaac tgctctacga
     5941 ttcaatctgt tgtgccatta aaagtattgc aagatgctta tggtttaaaa cgagtggcat
     6001 atacaacata tcaagctgta tcaggttcag ggatgaaagg taagaaagat ttagctgaag
     6061 gtgtaaatgg taaagcacca gaagcatatc cacatccaat ttataataat gtgttaccgc
     6121 atattgatgt gtttttagaa aacggatata caaaagaaga acaaaaaatg attgatgaga
     6181 cgagaaaaat tttaaatgcg ccagacttaa aagtaacagc aacatgcgca cgtgtgcctg
     6241 ttcaagatag tcatagtgtt gaaattgatg taacgcttga caaagaaacg acagcagaag
     6301 atattaaagc gttatttgat caagatgacc gcgttgtttt agtagacaat ccagagaaca
     6361 atgaatatcc aatggcaatc aattccacta ataaagatga agtgtttgtt ggccgtatac
     6421 gtagagatga ttcattagaa aatactttcc atgtatggtg tacatcagac aatttattaa
     6481 aaggtgctgc attaaatgct gtacaagtat tggaacaagt tatgcgttta aaaggagcga
     6541 attaaaatga cacatttatt tgagggtgtt ggcgttgcac ttacaacccc ttttacaaat
     6601 aacaaagtta atcttgaagc tttgaaagca cacgttaatt ttttactaga aaataatgcc
     6661 caagcaatca tcgttaatgg aactactgct gagagcccta ctttaacaac agatgaaaaa
     6721 gaacgcattc taaaaacagt tattgatctt gtagataaac gtgttcctgt catagctgga
     6781 actggaacta atgatactga aaagtcaatc caagcttcat tccaagctaa agccttaggg
     6841 gctgatgcaa ttatgttaat tacgccctac tacaacaaaa cgaaccaacg tggtttagtc
     6901 aaacactttg aagcgattac agatgctgtg aaattaccag tcgtgctgta caatgttcct
     6961 tcaagaacga acatgacaat tgaaccagaa actgtagaaa tattaagtca acatccttat
     7021 atagttgctt taaaagatgc tacgaatgat tttgagtatt tagaagaagt gaaaaagcga
     7081 attgatacaa attcatttgc attatatagt ggcaatgatg acaacgtcgt cgaatactat
     7141 caacgtggcg gtcaaggggt tatctctgtt attgccaatg tcattcctaa agaatttcaa
     7201 gcgttatacg atgctcaaca aagtggatta gatattcaag atcaatttaa accaatcggc
     7261 acactgttat cagccctatc agttgatatt aacccaattc ctattaaagc tttaacaagt
     7321 tatttagaat ttggaaacta tgaattacgt ttaccattgg ttagcctaga agatacagat
     7381 actaaagtgc ttcgtgaagc atatgacaca tttaaagcgg gtgaaaatga gtgaaaatat
     7441 tactaattgg ctatggtgca atgaatcaac gcgttgctag attagcagaa gaaaaaggac
     7501 atgaaatcgt tggggtcatt gaaaatacac cgaaagcaac aacgccatat caacaatatc
     7561 aacatattgc agatgttaaa gatgccgatg ttgcaataga tttttcaaat ccgaatctat
     7621 tattcccatt attagatgaa gagtttcatt tgccattagt tgtggcaaca actggcgaga
     7681 aagaaaaact acttaataag ttagatgaat tgagtcaaaa tatgcctgta tttttcagcg
     7741 cgaacatgag ttatggcgtt catgcactga ctaaaatttt agcagctgct gttcccctac
     7801 ttgatgattt cgacatcgaa ttgactgagg cacatcataa taaaaaagta gatgcaccaa
     7861 gtggtacgtt agaaaaattg tatgatgtga tcgtatcttt gaaagaaaat gtaacacctg
     7921 tgtatgatag acatgaatta aatgaaaaac gccagccaca agatattggt atacattcta
     7981 ttcgtggagg tacgattgtc ggtgaacatg aagttctatt tgctggcact gatgaaacga
     8041 ttcaaatcac acatcgtgca caatcaaaag atatttttgc gaatggtgct atacaagcag
     8101 cagaacgctt agttaataaa ccaaacggct tttatacgtt tgacaactta taaacatatt
     8161 aaaggagatc gattatttta tggtacaaca tttaacagct gaagaaatta ttcaatatat
     8221 aagtgatgct aaaaagtcta caccaataaa agtatattta aatggtaatt ttgaaggcat
     8281 cacataccca gaaagtttta aagtatttgg ttcagaacaa tctaaagtaa tcttttgtga
     8341 agcggatgat tggaaacctt tttacgaagc atatggtagt caattcgaag atatagaaat
     8401 tgaaatggat cgtcgcaatt ctgccattcc attaaaagac ttaacaaata cgaatgcacg
     8461 tattgaacca ggtgcattta ttagagaaca agccattatt gaagatggtg ctgtcgttat
     8521 gatgggcgca acaattaata ttggcgcagt cgttggcgaa ggtacaatga ttgatatgaa
     8581 tgctactctc ggtggtcgtg ctacaactgg taaaaatgta catgtagggg ctggcgcagt
     8641 attggcaggt gtgattgaac cccctagtgc atcaccagtt ataatcgagg atgatgtatt
     8701 aatcggtgca aatgcagtta ttttagaagg tgtacgtgtt ggtaaaggtg ctattgttgc
     8761 agctggcgcg attgtgacac aagatgtacc agctggtgca gttgttgctg gtacacctgc
     8821 aaaagtgatt aagcaagctt ctgaagtaca agatactaaa aaagagattg tagcagcatt
     8881 aagaaaactg aatgactagt gacgtcaacg tataataatt tcaaggttga gatacgatta
     8941 tgtctcaacc ttattgttta tcattgaata aacttatgat cacaaatttc gaaatacgtt
     9001 acactattta tatagtaatt taacacaaag gattgataat tatgaatgaa ttagaatttg
     9061 ttacgaaaca tcgccgtcat ttacatcaac atcctgaatt aagcttacat gaatttgaaa
     9121 caactgctta tattaaagcg tttttagata gtttaaatat taaatacgat tgcccattgg
     9181 aaactggcgt cattgcatac ttagaaggta atggctcaca tacgatagcg tatagagctg
     9241 atattgatgc gttacctatt ttagaggaaa atgatgtgcc ttatcgcagt caatctgatc
     9301 atgtgatgca tgcttgtgga catgatggtc atacaactgc attaatgctt tttgtacaac
     9361 gttgcaaaga catgcaagac gcaggtcaat taccgcaaaa tgtcgttttc attttccaac
     9421 ctgcagaaga aactggtggc ggtgcaaatc gattaataaa agccggtgcc tttgataagt
     9481 atccaattga agcggtattt ggtattcatg ttaacccatt tgctgatgaa ggcattgcag
     9541 tgataagaga tgaagaaatt acggccagcg caacagagta tcgctttttc ttaacaggcc
     9601 tgtcaagtca tgttgctgat aaagaacaag ctcattcttg tggtgaagca ttacaacatg
     9661 tattaactca aatatcacaa attcaacaat ttcaccttaa cggtttgaaa cgaaatattg
     9721 ttcatattgg tcattttaaa gctggtgaag cgattaacac tgtaccaagt aatggttatt
     9781 tagaaggtac aattcgtaca tatgatattg atgatttaac aatcgttaaa aatcaaatgc
     9841 acaagatagc agaaagtgtc aagcttctgt ttaatgtaga atgtgaagtt aaatttgcag
     9901 aaggttatcc ccctacaatc aatagtccga aattacgtac tcaaatagag gacgccttaa
     9961 taaaagctga tttaaatgtc tatgacaaac caacgccatt cttatttggg gaagatttta
    10021 gtttttatgg tcaacaacta gctccagctt actttgtttt tataggaaca cgaaatgaag
    10081 ataaaggttt tgtaactggt ttgcacacat cacatttaaa ttttgatgaa aaagcgttaa
    10141 taaacgtggt taatttttac gaaaatttat taaataatta caaagaggtg taatacattg
    10201 acagcaacat ggtctgtaaa taagaaaata tttttacaaa atgcaatcac agtcaaaaac
    10261 aatcagccat taatggcagt tgttaaaaat aatgcatatc actatgacct agaatttgct
    10321 gtaactcagt ttatccatgc aggtatagat acatttagca caacatcact acgagaagca
    10381 attcaaatta gacaacttgc tccagatgca acaatctttt taatgaatgc agtttacgag
    10441 tttgatttag tccgtgaaca tcaaatacac atgactttgc cgtcgttgac atattactat
    10501 aaccataaaa atgatttagc aggtattcat gttcacttag aatttgaaaa tttattacat
    10561 cggtctggat ttaaagattt aaacgaaatt aaagaagtat tgaaagatca ccatcataat
    10621 caaaatgcaa aaatgattat tagtggttta tggacccatt ttggatatgc tgatgaattc
    10681 gatgtgtcag attataatgt tgaacgttca caatggatgg aaattgttga agcactttta
    10741 tctgaaggtt atcagttcga cctaatccat gctcaaaata gtgcgagttt ttatcgggaa
    10801 ggacaaatat tactacccca ccatacacat gcgcgtgtag gtattgcgtt atacggttca
    10861 agaccatata gttcactgaa tcaacatgat atagttcagt cattaactgt aaaagcacat
    10921 gttattcaag tgcgcgaagt acaagctggt gattattgcg gttatagctt tgcctttgaa
    10981 gtgactaaaa acaatacaaa attagctgta gttgatatcg gttatggcga tggaatttta
    11041 agaactcgtg ctaaacatga agcacttatc aatggtaaac gctacccgat acgtgcatta
    11101 atgatgagcc atatgtttgt tgaagtagat ggcaatgtac atgcacaaga tgaagttatt
    11161 ctttataata atgatatccg catcgatgaa tataccttta aaggtgttgg tgcaaattct
    11221 gaacaattaa gtgctatgaa tcatgattct ttaaaaaagg agtacatttc aaatgactgt
    11281 taaatataat caaaatggcg aattaacaat ggatggtatt agtttaaaaa cgattgcaca
    11341 aagctttggt acacctacca ttgtttatga tgaactacaa attagagaac agatgcgccg
    11401 ttaccatcgc gcatttaaag atagtggatt aaaatacaat atttcatacg cctcaaaggc
    11461 atttacttgc attcaaatgg tcaaacttgt agctgaggaa gatttacagt tagatgttgt
    11521 ttctgaaggt gaattatata cagctttaga agcaggtttt gaaccgagtc gcatccattt
    11581 ccatggtaac aataaaacga aacatgaaat taggtatgct ttagaaaata atatcggtta
    11641 ttttgttata gattcattag aagaaattga attaatagac cgctatgcta atgatacggt
    11701 tcaagttgta ttacgagtta atccaggtgt tgaagcacat acacacgaat ttattcaaac
    11761 tgggcaagaa gatagtaagt ttggattatc aattcaatat ggcttagcta aaaaagcaat
    11821 tgacaaagtc caacaatcta aacacttaaa attaaaaggt gtacattgtc atattggttc
    11881 acagattgaa ggtacagaag catttattga aactgctaaa attgttttac gttggcttaa
    11941 agagcaaggc attcaagttg aattattaaa ccttggtggt ggctttggta ttaaatatgt
    12001 tgaaggtgac gaaagtttcc ctatcgaaag tggtattaaa gatattacag acgcaataaa
    12061 atccgaaatt aaagttctag gtatagatgc accagaaata ggtattgaac cgggacgatc
    12121 aattgtaggt gaagctggcg ttactttata tgaagttgga accattaaag aaattccaga
    12181 gattaataaa tatgtttcaa tcgatggcgg tatgagtgat catatcagaa ctgcacttta
    12241 tgacgcaaag tatcaagcat tgcttgttaa tagaaatgaa gaagcagatg acagtgtaac
    12301 tatagctgga aaattatgtg agtctggtga tatcattatt aaagacgcta aattaccttc
    12361 atcagtcaaa cgtggagact atcttgctat attatcaact ggtgcatatc attactctat
    12421 ggcatccaat tacaatcaaa tgcaaaagcc ttctgtgttt ttcttaaaag atggcaaagc
    12481 acgtgaagtt ataaagcgac aatcgttaag acaactcatt attaatgata caaaataaaa
    12541 ataaacaaag taatccccga agcacagaaa attatactgt gactcgggga atttttatgc
    12601 attatagatg actgtagaaa acatgcttgg acatatactc taaaaaagga agaacagcac
    12661 atttttagtt agaaagtatt tatttctttt tgtcattaat cctttatatt tagttagccc
    12721 tattcaacta atttaaagca tcatatacat accacacttt aaacaagcat catgaaatta
    12781 gattttatgc tttacttgct ttttcaaata atataaaacg ataagaatta acaacaaata
    12841 taatgtataa aacagaatt
//
LOCUS       DAHHSC010000056        12754 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.056, whole genome shotgun sequence.
ACCESSION   DAHHSC010000056 DAHHSC010000000
VERSION     DAHHSC010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 12754)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12754)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12754
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.056"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            27..944
                     /locus_tag="NBI57_001226"
     CDS             27..944
                     /locus_tag="NBI57_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HCD1822182.1"
                     /translation="MENKYTHGVLFYHEHSGLKNINQGIGEVTTALSSICKHLSIQLS
                     ENEGDIIKYCQEIKTKNYAKDVDILFILGGDGTVNELINGVMSHDLQLPIGILPGGTF
                     NDFTKTLNIAPNHKQASEQMISAQVGTYDVIKINNQYALNFVGLGLIVQNAENVQDGS
                     KDIFGKLSYIGSTVKTLLNPTQFNYQLSIDDKTYSGETTMILTANGPFIGGSRIPLTD
                     LSPQDGELNTFIFNEQSFSILNDIFKKRDSMNWNEITQGIEHIPGKKISLTTDPAMKV
                     DIDGEISLETPIDIEVIPNAIQLLTVNDL"
     gene            complement(1823..2365)
                     /locus_tag="NBI57_001227"
     CDS             complement(1823..2365)
                     /locus_tag="NBI57_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'(3')-deoxyribonucleotidase"
                     /protein_id="HCD1822183.1"
                     /translation="MTRKSIAIDMDEVLADTLGEIIDAVNFRADLGIKMEALNGQKLK
                     HVIPEHDGLITEVLREPGFFRHLKVMPHAQEVVKKLTEHYDVYIATAAMDVPTSFSDK
                     YEWLLEFFPFLDPQHFVFCGRKNIVKADYLIDDNPRQLEIFTGTPIMFTAVHNINDDR
                     FERVNSWKDVEQYFLDNIEK"
     gene            complement(2704..3762)
                     /gene="hisC"
                     /locus_tag="NBI57_001228"
     CDS             complement(2704..3762)
                     /gene="hisC"
                     /locus_tag="NBI57_001228"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HCD1822184.1"
                     /translation="MKEQLNQLSAYQPGLSPRALKEKYGIEGDLYKLASNENLYGPSP
                     KVKEAISAHLDELYYYPETGSPTLKAAISKHLNVDQSRILFGAGLDEVILMISRAVLT
                     PGDTIVTSEATFGQYYHNAIVESANVIQVPLKDGGFDLEGILKEVNEDTSLVWLCNPN
                     NPTGTYFNHESLDSFLSQVPPHVPVIIDEAYFEFVTAEDYPDTLALQQKYDNAFLLRT
                     FSKAYGLAGLRVGYVVASEHAIEKWNIIRPPFNVTRISEYAAVAALEDQQYLKEVTHK
                     NSVERERFYQLPQSEYFLPSQTNFIFVKTKRVNELYEALLNVGCITRPFPTGVRITIG
                     FKEQNDKMLEVLSNFKYE"
     gene            complement(4002..5516)
                     /locus_tag="NBI57_001229"
     CDS             complement(4002..5516)
                     /locus_tag="NBI57_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HCD1822185.1"
                     /translation="MTNFFDILSERKGQLFSTMIEHIQISFIALLIATAIAVPLGILL
                     TKTKTISEIVMNIAAILQTIPSLALLGLMIPLFGIGRVPAIIALVVYALLPILRNTYT
                     GIKEVDPSLIEAAKGIGMKPFRRLTKVELPIAMPVIMAGVRTAMVLIIGTATLAALIG
                     AGGLGDLILLGIDRNNASLILLGAIPAALLAIIFDLILRFMAKVSYKKLLMTLGVIVM
                     IIILAIAIPMFAQKGDKITLAGKLGSEPSIITNMYKILIEEETKNTVEVKDGMGKTAF
                     LFNALKSDDIDGYLEFTGTVLGELTKEPLKSKEEKKVYEQAKQSLEKKYQMTMLKPMK
                     YNNTYALAVKRDFAKQHNIRTIGDLNKVKDQLKPGFTLEFNDRPDGYKAVQKAYNLNL
                     DNIRTMEPKLRYQAINKGNINLIDAYSTDAELKQYDMVVLKDDKHVFPPYQGAPLFKE
                     SFLKKHPEIKKPLNKLENKISDEDMQMMNYKVTVKNEDPYTVAKDYLKAKGLIK"
     gene            complement(5509..6486)
                     /locus_tag="NBI57_001230"
     CDS             complement(5509..6486)
                     /locus_tag="NBI57_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCD1822186.1"
                     /translation="MIKFKNVTKRYGKHVAVDNISFNINEGEFFVLIGPSGCGKTTTL
                     KMINRLIHLSEGYIYFKDKPISDYPVYEMRWDIGYVLQQIALFPHMTIKENIAQVPQM
                     KKWKEKDIDKRVDELLDMVGLEPEKYKNRKPDELSGGQRQRVGVIRALAADPPVILMD
                     EPFSALDPISREKLQDDLIELQTKIKKTIIFVTHDIQEAMKLGDKICLLNEGHIEQID
                     TPEGFKNNPQSEFVKQFMGSHLEDDAPCVEENAIIRDLDIMKPIDEVTSMSAYPIVYD
                     NQPIEVLYQHLSESERVIVMQEDSVGQYVIDRKDIFKYLSQKKEVAQHD"
     gene            complement(6708..8489)
                     /gene="recQ"
                     /locus_tag="NBI57_001231"
     CDS             complement(6708..8489)
                     /gene="recQ"
                     /locus_tag="NBI57_001231"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HCD1822187.1"
                     /translation="MMQQTLSHYFGYETFRPGQEEIISKVLDHRNVLGVLPTGGGKSI
                     CYQVPGLLLGGTTIVISPLISLMKDQVDQLKAMGIQAAFLNSSLTQKEQQRIEKALSN
                     GEIQFLYVAPERFENRYFLNMLQRIKIHLVAFDEAHCISKWGHDFRPSYQNVISKVFT
                     LPQDFTIIALTATATVEVQQDIREKLNIAQTDQIKTSTKRRNLIFKVNPTYQRQKFIL
                     DYIKTHDEDAGIIYCSTRKQVEELQEALESQKIESVIYHAGLSNKEREEAQNDFLFDR
                     VKVVVATNAFGMGIDKSNVRFVIHYNMPGDLESYYQEAGRAGRDGLKSECILLFSERD
                     INLHEYFITVSQADDDYKDKMGEKLTKMIQYTKTKKCLEATIVHYFEPNEKLEECEQC
                     SNCVQQDKSYNMTQEAKMIISCIARMKQQESYSVIIQVLRGESTDYIKYKGYDQISTH
                     GLMKGYTTSELSHLIDELRFKGFLNENDEILMCDTSIKKLLSNEVEVFTTPFKQKATE
                     KVFINTVEGVDRVLFSQLVEVRKKLSDKLTIAPVSIFSDYTLEEFAKRKPASKQDMIN
                     IDGVGSYKLKHYCPAFLETIQNYKAKV"
     gene            complement(8501..10384)
                     /locus_tag="NBI57_001232"
     CDS             complement(8501..10384)
                     /locus_tag="NBI57_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HCD1822188.1"
                     /translation="MGMEAYKIEHLNKSYADKTIFDNLDLSISEGEKIGLVGINGTGK
                     STLLKVIGGIDDDFTANVMHPNQYRIRYSSQKQDLNEDMTVFDAVLSSDTTTLRIIKQ
                     YEQAVQAYADDQSDKLFKRMIDAQDAMDQHDAWDYNAEIKTILSKLGIHDTTKYIKEL
                     SGGQQKRVVLAKTLIEQPDLLLLDEPTNHLDFESISWLINYVKQYPHTVLFVTHDRYF
                     LNEVSTRIIELNRGKLASYPGNYESYIEMRAEREVTLQKQQQKQRALYKEELAWMRAG
                     AKARTTKQQARINRFNDLENEVNQQYKDDKGELNLAYSRLGKQVFELEDLSKAINDKV
                     LFEHLTEIIQKGERIGVVGPNGAGKTTLLNILSGEDQQFEGKLKTGQTVKVAYFKQTD
                     ETLDRDIRMIDYLREESEVAKEKDGTSVSITQLLERFLFPSATHGKKVYKLSGGEQKR
                     LYLLRLLVHQPNVLLLDEPTNDLDTETLTILEDYIHTFGGTVITVSHDRYFLNKVAQS
                     YWFIHDGQMEKIIGTFEDYESYKKSLDKNKSTLKQQSKSYTTVRKKNGLSYKEKLEYE
                     QLMKRIEQAEVRMEEIDVLMIEASADYGKIKELNEEKEQLEIQYDLDITRWSELEEIK
                     EQQ"
     gene            complement(10654..12594)
                     /gene="ltaS"
                     /locus_tag="NBI57_001233"
     CDS             complement(10654..12594)
                     /gene="ltaS"
                     /locus_tag="NBI57_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyglycerol-phosphate lipoteichoic acid
                     synthase LtaS"
                     /protein_id="HCD1822189.1"
                     /translation="MSSQKKKISLFAFFLLTVITITLKTYFSYYVDFSLGVKGLVQNL
                     ILLMNPYSLVALVLSVFLFFKGKKAFWFMFIGGFLLTFLLYANVVYFRFFSDFLTFST
                     LNQVGNVESMGGAVSASFKWYDFVYFIDTLVYLFILIFKTKWLDTKAFSKKFVPVVMA
                     ASVALFFLNLAFAETDRPELLTRTFDHKYLVKYLGPYNFTVYDGVKTIENNQQKALAS
                     EDDLTKVLNYTKQRQTEPNPEYYGVAKKKNIIKIHLESFQTFLINKKVNGKEVTPFLN
                     KLSSGKEQFTYFPNFFHQTGQGKTSDSEFTMDNSLYGLPQGSAFSLKGDNTYQSLPAI
                     LDQKQGYKSDVMHGDYKTFWNRDQVYKHFGIDKFYDATYYDMSDKNVVNLGLKDKIFF
                     KDSANYQAKMKSPFYSHLITLTNHYPFTLDEKDATIEKSNTGDATVDGYIQTARYLDE
                     ALEEYINDLKKKGLYDNSVIMIYGDHYGISENHNNAMEKLLGEKITPAKFTDLNRTGF
                     WIKIPGKSGGINNEYAGQVDVMPTILHLAGIDTKNYLMFGTDLFSKGHNQVVPFRNGD
                     FITKDYKYVNGKIYSNKNNELITTQPADFEKNKKQVEKDLEMSDNVLNGDLFRFYKNP
                     DFKKVNPSKYKYETGPKANSKK"
BASE COUNT         3997 a         2309 c         1800 g         4648 t
ORIGIN      
        1 ccaattttta gaaagtaggc taaactatgg aaaataaata tacacatggt gtcctctttt
       61 accatgaaca cagtggatta aaaaatatta atcaaggtat tggggaagtt acaacagcat
      121 taagttcaat ttgtaagcat ctttctattc aattgagtga aaacgaaggt gacattatta
      181 aatattgcca agaaatcaaa actaaaaact atgcaaaaga tgtagatata ttatttattc
      241 ttggcggtga tggcacggtt aacgaactta taaatggtgt tatgtcacat gaccttcaac
      301 tccctattgg cattttacca ggcggtactt ttaatgattt tacaaaaacg ctaaatatag
      361 cacctaatca taaacaagct agtgaacaaa tgatttctgc acaagttggt acatacgatg
      421 taattaaaat aaataatcaa tatgcactca actttgttgg cttaggtctc attgttcaaa
      481 acgctgaaaa cgtacaagat ggttcaaaag atatatttgg taaattgagt tatattggat
      541 caactgtcaa aacactgcta aatccaacac aatttaatta tcaattatca atagatgata
      601 agacatattc tggcgaaacg accatgatac taacagcaaa tggtccattc attggtggta
      661 gtcgtatacc attgactgat ttgtctcctc aagatggtga actgaacact ttcattttta
      721 atgaacaaag tttcagtatt ttaaatgata tttttaaaaa gcgtgatagc atgaattgga
      781 atgaaattac tcaaggtatt gagcatatac ctggtaaaaa gatttcactt acaacagatc
      841 cagcaatgaa agttgatatt gatggcgaaa tttcattaga aacacctatt gatattgagg
      901 ttataccaaa tgctattcag ttacttactg tcaatgattt gtaagcatcc ggaatgatct
      961 tgacccgata ttatattagg tttggagtgt aatcataaga ttcaacgcac cttatattcg
     1021 tttttattag ctttaagcta ttaccctgtt tcaaaaacag acatataatt ttggcgcatg
     1081 ccactaaaat gattgatgtt gtattttaaa tcatagtggt ttatgcgtct ttttcaaatt
     1141 ctataaaaaa tcggatgacg tgtaatctgc catagattaa cacattcatc cgatttataa
     1201 taatgagata gactaacatt tattgagagc gggacggaaa tgataaagaa cgactaatga
     1261 ttgattatgt agcgattctt tatcattagt caaagctaat gtgtacttaa aaataggagg
     1321 gagtgggaca gaaatgatat tttcgcaaaa tttatttcgt cgtcccaccc caacttgcat
     1381 tgtctgtaga aattgggaat ccaatttctc tgtgttgggg ccccgccggc aaggttgact
     1441 agaattgaaa aaagcttgtt acaagcgcat tttcgttcag tcaactactg ccaatataac
     1501 ttcgtagagc atagaacatt gatttatgtc ccactcccat aagaaatact aatttctaaa
     1561 gaaaaagtat ttctttatgt tggggcccac cccaacttgc attgtctgta gaaattggcg
     1621 ttccaatttc tctatgttgg ggccccgcca acttgcattg tctgtagaaa ttgggaatcc
     1681 aatttctcta tgttggggcc ccgcagtcaa ctactgccaa tatagtgttg tagtgtctaa
     1741 gacataaaag ttcatctcag tcacaattac tttatagcct atccatcatc ttttctacat
     1801 gaaatttttc aagtgatata ttttatttct caatattatc taaaaaatac tgttctacat
     1861 ctttccagct atttacgcgt tcaaatcgat catcattaat attatgcact gctgtaaaca
     1921 taatcggtgt accagtaaaa atttcaagct gtctaggatt gtcatctatt aaataatcag
     1981 ctttaacgat gttttttcta ccacaaaaaa caaaatgctg aggatctaaa aatggaaaga
     2041 actctagtaa ccattcatat ttatcactaa atgatgttgg tacatccatt gctgctgtag
     2101 caatatatac atcataatgt tcagttaatt ttttcacaac ttcttgtgca tgtggcatca
     2161 ctttaagatg tctgaagaag cctggttctc tcaatacttc tgtaattaat ccatcatgtt
     2221 caggaataac atgtttaagt ttttgaccat tcaaagcttc cattttaata cctaaatccg
     2281 ctctaaaatt gacagcatca atgatttctc ctaatgtatc tgccaatact tcatccatat
     2341 caatcgcgat tgatttacgg gtcattttaa tcttctcctc aaaatatcag tattgattca
     2401 gtatagcaaa aaagcaattg atacaaaaca atatcatcta ttgttgcttt ttacactgaa
     2461 tactctcacc tcatcattca cactgcattg agcttgagac ataaatcaat gttcaatgct
     2521 ctacaaagtt atattggcag tagttgactg aacgaaaatg cgcttgtaac aagctttttt
     2581 caattctagt caggggcccc aacacagaga atttcgaaaa gaaattctac aggcaatgca
     2641 agttggggtg ggacgacgaa ataaattttg agaaaatatc atttctgtcc cactccccac
     2701 ttactattcg tatttaaaat ttgataaaac ttctaacatt ttatcatttt gttctttaaa
     2761 accaattgta attctaacac cagttggaaa tggtcgcgta atacacccta catttaaaag
     2821 tgcttcataa agttcattta cccgctttgt ttttacaaat ataaaattcg tttgacttgg
     2881 caagaaatac tcactttgag gtaattgata aaatctttcg cgttcaacac tatttttatg
     2941 tgttacctct tttaaatatt gttgatcttc aagtgctgca actgctgcgt attcagatat
     3001 acgtgtcaca ttaaatggtg gtctaatgat gttccatttt tcaatcgcat gttcacttgc
     3061 taccacatat cctacacgta aacccgctaa tccatacgcc tttgaaaatg tacgtaataa
     3121 gaaagcattg tcatattttt gttgcaaagc aagtgtatcc gggtagtcct ctgctgtcac
     3181 aaattcaaaa taagcttcat ctataattac tggtacatgt ggaggtactt gagataaaaa
     3241 cgaatctaag ctctcatgat taaaatatgt acctgtagga ttatttggat tacataacca
     3301 taccaatgac gtatcttcat taacttcttt taaaatacct tctaaatcga agccaccatc
     3361 ttttaaaggt acttgtatca cattagctga ttcaacaatc gcattgtgat aatattgacc
     3421 gaatgtcgct tcacttgtaa caatagtatc ccctggcgtt aatactgctc tagaaatcat
     3481 taatataact tcatctaatc ccgcaccaaa taaaatgcgt gattgatcta catttaaatg
     3541 tttactaatc gccgctttta atgtcggtga tcctgtttca ggataataat ataactcatc
     3601 taaatgtgct gatatcgctt ctttaacttt aggcgatggt ccatacaaat tttcatttga
     3661 tgcaagttta tataaatctc cttcaatgcc atacttttct ttcaatgccc ttggagataa
     3721 accaggctga tatgctgata gttgattaag ttgttctttc ataataaaaa caacctcctt
     3781 agaattaact gaaattttaa aaaacttata ttcattatat cgctctatgt ttctaaaatg
     3841 caatgctact attttcacat cataatagct tacattgtgt ttaacatgtc atatttttta
     3901 atcattattg catgtttaaa ttgaacataa ttatagattt aaatataaca acgccacata
     3961 agatataatt tggtgttacg catcttatgt ggcgttggtc gttatttgat taaccctttt
     4021 gcttttaaat aatctttcgc aactgtatat gggtcttcat ttttaactgt tactttatag
     4081 ttcatcattt gcatatcttc atcagatatt ttgttttcta gtttgtttaa cggtttctta
     4141 atttctggat gtttctttaa aaagctttct ttaaataatg gtgctccttg atatggtgga
     4201 aatacgtgct tatcatcttt taacacaacc atatcatatt gttttaattc agcgtcagtt
     4261 gaatatgcat ctattaaatt aatattacct ttattgatcg cttgatatct caacttaggt
     4321 tccattgtac gtatgttatc taaatttaaa ttataagcct tttgaacagc tttgtaacca
     4381 tctggacgat cattaaattc caatgtaaat cctggtttaa gttgatcttt aaccttattt
     4441 aaatcaccaa ttgtacgtat attatgttgt ttagcaaaat cacgttttac agctaaagca
     4501 tacgtattgt tatacttcat tggttttaac atagtcattt gatatttctt ttcaagactt
     4561 tgcttagctt gttcataaac ttttttctct tcttttgact tcaatggttc ttttgttaat
     4621 tcacctaaaa ctgttccagt aaactctaaa tacccatcta tatcatcaga ttttaaagca
     4681 ttaaataaaa atgctgtttt ccccatacca tctttcactt ctacagtatt tttggtctct
     4741 tcttctatta aaattttata catatttgta ataatcgaag gctcggagcc aagctttcca
     4801 gctaacgtaa ttttatcacc tttttgtgca aacataggaa tagcgatggc cagtataata
     4861 atcatcacta taacacctaa cgtcatcaat aactttttat aagatacttt agccataaat
     4921 cttaaaatta aatcaaatat aattgccaat aaggctgctg gaattgcacc taataatatc
     4981 aatgacgcat tgttacggtc tatacctaat aaaattaaat ctcctagtcc gcctgcacca
     5041 attaatgctg ctagtgtcgc tgtacctata attaatacca tagccgttct tacaccagcc
     5101 attataacag gcattgctat cggaagttcg actttagtta aacgtctaaa tggtttcata
     5161 cctatacctt tagccgcttc aatgagtgat ggatcaactt ctttaattcc agtatacgta
     5221 ttccttaaaa ttggtaacaa cgcatacact acaagtgcaa taattgctgg cacacgaccg
     5281 ataccaaata aaggaatcat taaacctaat aatgccaacg atggtatggt ttgaagaatt
     5341 gccgcaatat tcattacgat ttcagatatc gttttagtct tcgttaataa aatacctaat
     5401 ggtaccgcaa tagcagttgc aatcaataat gcaataaatg atatttgaat atgttctatc
     5461 attgtcgaaa agagttgccc cttacgttca ctcaatatgt cgaaaaagtt agtcatgttg
     5521 agctacctcc tttttctggg acaaatattt gaagatatct ttcctatcaa taacatattg
     5581 acctacgcta tcttcttgca tgacaatgac acgctcgctc tctgataaat gttgatacaa
     5641 tacttcaatt ggttgattgt cataaacaat tggataagcg ctcatagatg taacctcatc
     5701 gattggtttc ataatatcca agtcacgaat aattgcgttc tcttcaacac atggcgcatc
     5761 atcttctaaa tgactaccca taaattgttt aacaaattca ctttgaggat tatttttaaa
     5821 tccttctggt gtgtcaattt gttcaatatg cccttcattc aaaagacaaa tcttatcacc
     5881 aagtttcatc gcctcttgaa tatcatgtgt aacaaatatg attgtcttct taattttagt
     5941 ttgtaattca attaaatcat cttgaagttt ttctcggctg attgggtcta atgcactaaa
     6001 cggttcatcc attaaaataa ctggtggatc agctgctaac gcacgtataa ctcctacacg
     6061 ttgtcgttgc ccccctgaca attcatcagg ttttctgttt ttatattttt caggttctaa
     6121 tccaaccata tcaagtaatt catctactct tttatctata tctttttctt tccacttttt
     6181 catttgtggc acttgtgcaa tattttcttt gatcgtcata tgtgggaata atgcaatctg
     6241 ctgcaatacg tatccaatat cccaacgcat ttcgtatact ggataatcac ttattggttt
     6301 atctttaaaa taaatataac cttcacttaa gtgaatgagt cgattaatca tttttaatgt
     6361 cgtagttttt ccacaacctg aaggtccaat tagcacaaaa aattcaccct cattaatatt
     6421 gaaactaatg ttatcgacag caacatgttt gccataacgc ttagttacat ttttaaactt
     6481 aatcactttg ccacctcttt ttttctcata gcataaaacc gagattatat gtatgtattc
     6541 cctatttaac cacgtttatt acaattttca aatttaaatg atttatcctt gaactttttt
     6601 aacaaaataa tgaataatag gtaatctcca gttaagaaat agtgttattt taccttgaat
     6661 tcaaaaaaac acccagtaaa acaaggaatg cttactaggt gccttcacta tactttggct
     6721 ttataatttt gaatcgtttc taaaaatgct ggacaataat gttttaattt gtaactacct
     6781 acgccatcaa tattaatcat atcttgtttc gaagcaggct tacgtttagc aaattcctcc
     6841 aacgtgtaat cagaaaatat acttacaggt gctatcgtta atttgtcact taacttttta
     6901 cgaacttcta ccaactgact gaataatact cggtcaaccc cttcaaccgt atttataaat
     6961 actttttcag tcgctttttg cttaaatggt gttgtgaata cttctacttc attactgagt
     7021 aattttttaa ttgaagtatc acacattaat atttcgtcat tttcatttaa gaaccctttg
     7081 aatcttaatt catctattaa gtgacttaat tctgatgttg tgtaaccttt cattaaacca
     7141 tgggttgaaa tttggtcata acctttatac ttaatataat ctgttgactc tcctcttaac
     7201 acttgaatga taacactata actctcttgt tgtttcatac gagcgatgca actaataatc
     7261 atcttagctt cttgtgtcat attatatgat ttatcttgtt gaacacaatt actacattgt
     7321 tcacattctt ctaatttttc attcggttca aaataatgga caattgttgc ttctagacat
     7381 ttttttgttt ttgtatattg aatcatttta gttaactttt cgcccatttt atctttatag
     7441 tcatcatcag cttgagagac tgttataaaa tactcgtgta aattgatatc gcgttcgcta
     7501 aataacaaaa tacattcact tttcaacccg tcacgacctg cacgacccgc ttcttgataa
     7561 taagattcta aatctccagg catattataa tgaataacaa aacgtacatt ggatttatca
     7621 atacccatac caaaagcatt tgtagcaacg actactttaa cacgatcaaa taagaaatca
     7681 ttctgcgctt cttctctttc tttattgctc aaacctgcat gatatataac actttcaatt
     7741 ttctgacttt ctaaggcttc ttgaagctct tcaacttgct tacgtgtaga acaataaata
     7801 atacctgcat cttcatcatg tgttttaata taatccaata taaatttttg acgttgataa
     7861 gtaggattta ctttaaaaat taagtttcta cgcttagtac tcgttttaat ttgatcagtt
     7921 tgagcgatat ttaacttttc tctaatatct tgctgtactt caaccgtggc agttgctgtc
     7981 aacgctatta ttgtaaaatc ttgaggtaac gtaaatactt ttgaaataac attttggtaa
     8041 ctcggcctga aatcatgacc ccatttagaa atacaatgcg cttcatcaaa cgcgactaag
     8101 tgaatcttta tacgctgaag catatttaaa aaatatcggt tttcaaatcg ttctggtgca
     8161 acatacaaaa attgaatttc tccatttgat aatgcttttt caatacgttg ttgctctttt
     8221 tgagtcaaac tactatttaa aaaagcagct tgaattccca tcgcttttaa ttgatccact
     8281 tgatctttca ttaatgatat tagtggactt attacaattg ttgtaccacc taacaataaa
     8341 cctggtactt gatagcatat agacttacct ccaccagttg gtaagacacc aagcacatta
     8401 cgatggtcta atactttgct aataatttct tcttgtcctg gtcgaaatgt ttcataccca
     8461 aagtaatgcg ataatgtttg ttgcatcata aattgacccc ttattgttgt tctttaattt
     8521 cttctaactc actccatctt gtgatgtcta aatcatattg aatttcaagt tgttcttttt
     8581 cttcgtttag ttctttaatt ttcccataat ctgcacttgc ctcaatcatg agcacatcaa
     8641 tttcttccat tcttacttcc gcttgttcta tgcgtttcat caattgttca tattctaatt
     8701 tttctttata tgataaacca tttttcttac gtacagttgt ataagattta gattgttgct
     8761 tcaatgtgga tttattttta tctaatgatt ttttataact ttcataatct tcaaaagttc
     8821 cgataatctt ttccatctga ccatcatgaa taaaccaata tgactgtgca actttattta
     8881 agaagtagcg atcatggctt acggtaatca ctgtaccacc gaaagtatga atataatctt
     8941 caagtattgt taaagtctca gtatctaaat catttgtcgg ttcatctaac aacagaacat
     9001 ttggctggtg tacgagtaga cgtaataaat acaaacgctt ttgctctcca ccagataatt
     9061 tataaacttt tttaccatga gttgcacttg gaaataaaaa tcgttcaaga agttgtgtaa
     9121 tagataccga ggttccatct ttttcttttg cgacctcact ttcttctctt aaataatcaa
     9181 tcatacgaat atctctatcc agggtctcat ctgtttgctt aaaataagct actttaaccg
     9241 tctgcccagt cttcaattta ccttcgaatt gttggtcttc tccactcaaa atatttaaga
     9301 gtgttgtttt accagctcca tttggcccaa caacaccaat acgctcacct ttttgaataa
     9361 tttccgtcag atgttcaaat aatactttat cattaatagc ctttgataag tcttctaatt
     9421 cgaacacttg cttacctaat cttgaataag caagattcaa ttcaccttta tcgtctttat
     9481 attgctggtt aacttcattt tctaggtcat taaatcgatt aattctagct tgttgctttg
     9541 tagtacgagc cttagctcca gccctcatcc aagcaagttc ttccttatat aaagctcgtt
     9601 gcttttgttg ttgcttttga agtgttactt ctctttcagc gcgcatttca atataagatt
     9661 catagttacc aggatatgac gctaacttac ctctgtttag ttcaataatt ctagtggaaa
     9721 cttcatttaa aaaatatcga tcatgggtta cgaataaaac tgtatgagga tattgcttca
     9781 cataattgat caaccagctg attgattcga agtctaaatg gttcgtaggt tcatctaata
     9841 acaataaatc tggttgttct attaatgttt tagcaagtac aacacgtttt tgttgtccgc
     9901 cggataattc tttaatgtat ttagtagtat catgtatacc tagttttgag aggattgttt
     9961 taatttcagc gttatagtcc caagcatcat gttgatccat agcatcttgc gcatctatca
    10021 ttcgcttgaa caatttatca ctttggtcat ccgcataagc ttgtactgcc tgctcatatt
    10081 gcttgatgat gcgtaaagtt gttgtatcag aacttaatac tgcatcaaaa actgtcatat
    10141 cttcattaag gtcctgtttc tgagacgaat atcgaattcg atattgattt ggatgcataa
    10201 cattggctgt aaaatcatca tcaataccac caattacttt taacaacgta cttttccctg
    10261 taccatttat gcccaccaaa cctatttttt caccttctga aattgataaa tctaggttat
    10321 cgaatatagt cttatcggca taagatttat ttaaatgttc aattttatat gcttccatac
    10381 ccatcttcct tcttctgttg aaatctctac gtacttaaat cagttctata cattatacac
    10441 ctttttgaaa tcaaaagaaa tcgataccgt taagtgtcat aatttaatat attgttgctt
    10501 caactataaa taaaacttga taaggctttg atgactatgc taatgatttt tataatatac
    10561 ctttttatca acacatatac tgtaaaacat aatataaaag aacataataa aacccggatt
    10621 gatattttat caatccgagt tcgtgtttaa atattatttt ttagagtttg ctttaggtcc
    10681 tgtttcatat ttatacttcg aaggatttac ctttttgaag tctggatttt tgtagaatct
    10741 aaacaaatca ccattaagca cgttgtcact catttcgaga tccttttcaa cttgcttttt
    10801 attcttttcg aaatcagctg gttgagtagt tatgagttca ttatttttat tagaataaat
    10861 cttaccatta acatatttat aatcttttgt tataaagtca ccatttctga atggaactac
    10921 ttgattatga cctttagaga ataaatcagt accgaacatt aaataattct tcgtatctat
    10981 accagccaaa tgtaaaattg ttggcattac atcgacttga ccagcatatt cattattgat
    11041 accaccagat ttaccaggga ttttaatcca gaaaccagtt ctgtttaaat ctgtaaattt
    11101 agctggtgtg attttttcac ctaatagttt ttccatggca ttgttatggt tttcagagat
    11161 accatagtgg tcaccataaa tcataatcac tgaattgtca tataatcctt ttttcttcaa
    11221 gtcattaata tattcttcta atgcttcgtc taaataacgc gctgtttgaa tataaccatc
    11281 aactgttgca tcacctgtgt ttgacttctc aatagtcgca tccttttcat ctaatgtgaa
    11341 tggatagtgg ttagtcaatg taattaaatg agaatagaat ggtgatttca tcttagcttg
    11401 ataattagca gaatctttaa agaaaatttt gtctttcaag cctaagttta caacgttttt
    11461 atctgacatg tcatagtatg ttgcatcata gaatttatcg ataccaaagt gtttatatac
    11521 ttggtctctg ttccagaatg ttttatagtc accgtgcatg acatcagatt tgtagccttg
    11581 cttttgatct aaaattgctg gtaatgactg atacgtatta tctcctttta atgaaaaggc
    11641 agaaccttgc ggtaaaccgt ataaactgtt atccattgta aattcagagt cagatgtttt
    11701 accttgacct gtttgatgga aaaagttagg gaagtatgtg aattgctctt tcccacttga
    11761 taatttgttt aaaaacggtg ttacttcttt accattaacc tttttattaa ttaagaaggt
    11821 ttggaaactt tctaaatgaa tcttaataat atttttcttc tttgccaccc cataatattc
    11881 tgggttaggc tctgtttgac gttgtttcgt ataatttaat acttttgtta agtcatcttc
    11941 agatgctaac gctttttgtt gattattttc gatagtttta acaccatcgt atactgtaaa
    12001 gttataaggt cctaaatatt tcactaaata tttatggtca aatgtacgtg ttaataattc
    12061 tggtctgtca gtttcagcaa aagctaagtt taagaagaat aatgctactg aagctgccat
    12121 tacgacagga acaaatttct tactaaatgc ttttgtgtct aaccattttg ttttaaatat
    12181 taaaatgaat aagtaaacta acgtatcaat gaaataaaca aagtcatacc atttgaatga
    12241 tgcactaacc gcaccaccca tagattctac gttacctact tggtttaaag tactaaacgt
    12301 taaaaaatca gagaagaatc taaagtacac aacattggca tataatagga acgtcaataa
    12361 gaagccgcct atgaacatga accaaaatgc ttttttgcct ttaaagaata ggaacacact
    12421 taaaaccagt gctactaaac tataaggatt catcaataat attaagtttt gtactaaacc
    12481 tttaacacct aaagaaaaat caacataata agaaaaatac gtcttcaagg taatcgttat
    12541 tacggttaat aagaagaacg caaaaagact aattttcttt ttttgtgaac tcatgattct
    12601 ttcccccgtt atttagataa taaatcttgc gaaagtactt gaataaatta gatattaaca
    12661 ttatgtagtt aattaataag tgcgtttaaa agttaacatt gtcataatta tagtaattac
    12721 tttcagattc aagtttataa ttttcattac ttgt
//
LOCUS       DAHHSC010000057        12256 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.057, whole genome shotgun sequence.
ACCESSION   DAHHSC010000057 DAHHSC010000000
VERSION     DAHHSC010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 12256)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12256)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12256
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.057"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            240..443
                     /locus_tag="NBI57_001234"
     CDS             240..443
                     /locus_tag="NBI57_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sterile alpha motif-like domain-containing
                     protein"
                     /protein_id="HCD1822190.1"
                     /translation="MTFYNFIMGFQNDNTPFGILAEHVSEDKAFPRLEERHQVIRAYV
                     MSNYTDHQLIETTNRAISLYMAN"
     gene            complement(440..676)
                     /locus_tag="NBI57_001235"
     CDS             complement(440..676)
                     /locus_tag="NBI57_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1822191.1"
                     /translation="MDHHNQCNYVNPQNVSLDWECFIISKSEMLLDGVPNELINTWLD
                     KDIITPFSIRNDEINFKTKDIWDALIHHNWYYSN"
     gene            901..1482
                     /locus_tag="NBI57_001236"
     CDS             901..1482
                     /locus_tag="NBI57_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HCD1822192.1"
                     /translation="MAKTLYLMRHGQTLFNFKGLIQGFGDSPLTELGIAQAQKARSYY
                     ETKGINFDLYASSTQERASDTLENVAPNQSYQRFKGLKEWHFGIFEGESVYLFDNLYK
                     PEDLFGDRIVPFKGEARQQVEERIVKTLHDIMSQTKNNALVVSHGTIMGVFLRYCLKL
                     DEALKHNIGNCNILKFEYDNGTFKFVELIDPNL"
     gene            complement(1555..2172)
                     /locus_tag="NBI57_001237"
     CDS             complement(1555..2172)
                     /locus_tag="NBI57_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid transporter"
                     /protein_id="HCD1822193.1"
                     /translation="MVTAIIHGFILAIGLILPLGAQNVFIFNQGANQPKYRYVLPAII
                     TAGLSDSLLIIIAVVGVSIIIMSLPVLQAIIYIVGLIFLMYMAWTIWHDKPSTDGEAQ
                     IISPMKQVSFALSVSLLNPHAILDTIGVIGSSAALYSGSNKIAFTIACISVSWLWFFL
                     LAILGKMVGSIDKTGKLLTIINKISSIIIIIVALMILQKLIQLLF"
     gene            complement(2327..2821)
                     /locus_tag="NBI57_001238"
     CDS             complement(2327..2821)
                     /locus_tag="NBI57_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCD1822194.1"
                     /translation="MIEIKTLTNNDFNEYKRLVSTVNEEFTQDSHYSQTMTDTLIHDI
                     LNQGSPKCIVFGCYENETLIATAALEQIRYVGKEHKSLIKYNFVTNNDKSINSELINF
                     IINYARQNNYESLLTSIVSNNIGAKVFYSALGFDILGFEKNAIKIGNTYFDEHWLFYD
                     LINK"
     gene            complement(3220..3642)
                     /locus_tag="NBI57_001239"
     CDS             complement(3220..3642)
                     /locus_tag="NBI57_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HCD1822195.1"
                     /translation="MAIHYETKATNVGGRKGHVYTDDRALDIDIVPPAQADGKATNPE
                     QLFAAGYASCFNGAFDLILKQNKVRDAHPEVTLTVRLEDDSDSESPKLSVSIDATIKN
                     VISQEEAEKYLQMAHEFCPYSKATQGNINVDLNVNVVD"
     gene            3790..4506
                     /locus_tag="NBI57_001240"
     CDS             3790..4506
                     /locus_tag="NBI57_001240"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HCD1822196.1"
                     /translation="MTHVEVVATIAPQLSIEETLIQKINHRIDAIDVLELRIDQIENV
                     TVDQVAEMITKLKVMQDSFKLLVTYRTKLQGGYGQFINDLYLNLISDLANINGIDMID
                     IEWQADIDIEKHQRIIKHLQQYNKEVVISHHNFESTPPLDELQFIFFKMQKFNPEYVK
                     LAVMPHNKNDVLNLLQAMSTFSDTMDCKVVGISMSKLGLISRTAQGVFGGALTYGCIG
                     EPQAPGQIDVTDLKAQVTLY"
     gene            4589..5128
                     /locus_tag="NBI57_001241"
     CDS             4589..5128
                     /locus_tag="NBI57_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase"
                     /protein_id="HCD1822197.1"
                     /translation="MELQQAIANRRSVKKFKRDMHIDDALLYQAIEKAADAPNHGMRE
                     PWRVVHVPKDRLGDMSKDISKFAFPNELDKQQCHYDAVTKLGGMLLLILKTDPRQRQN
                     DENYFAFGAYAQNLMLLLYEAGIGTCWKSPLYIYDPKVRKTLGIKKDEVLAGFLYLTD
                     LEEDMPKAPRKNRNLITLY"
     gene            complement(5279..5599)
                     /locus_tag="NBI57_001242"
     CDS             complement(5279..5599)
                     /locus_tag="NBI57_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HCD1822198.1"
                     /translation="MQAIKSNESFKSIINSDTPVIVKFEAGWCPDCRAMDLWIDPIVE
                     QYNDYQWYTVNRDELEDVVVENEVMGIPSLLVFKNGDKIAHLHSANAKSPEQVESFLA
                     ETFK"
     gene            5743..6099
                     /locus_tag="NBI57_001243"
     CDS             5743..6099
                     /locus_tag="NBI57_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HCD1822199.1"
                     /translation="MIKFYQYKNCTTCKKAAKFLDEYGVSYEPIDIVQHTPTINEFKT
                     IIANTGVEINKLFNTHGAKYRELDLKNKLQTLSDDEKLELLSSDGMLVKRPLAVMGDK
                     ITLGFKEDQYKETWLA"
     gene            6257..6637
                     /gene="gcvH"
                     /locus_tag="NBI57_001244"
     CDS             6257..6637
                     /gene="gcvH"
                     /locus_tag="NBI57_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001831963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HCD1822200.1"
                     /translation="MAVPNELKYSKEHEWVKVEGNVATIGITEYAQSELGDIVFVELP
                     ETDDEINEGDTFGSVESVKTVSELYAPISGKVVEVNEELEDSPEFVNESPYEKAWMVK
                     VEISDESQLEALLTAEKYSEMIGE"
     gene            6816..7694
                     /locus_tag="NBI57_001245"
     CDS             6816..7694
                     /locus_tag="NBI57_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1963 domain-containing protein"
                     /protein_id="HCD1822201.1"
                     /translation="MLNIQDVSHLSKKEQKAYNRFVESVENGNLPVLPCIEMDLKEMQ
                     EETLSQSKIGGMPFLKSFKDIPLDENNVPMVLLAQINLNDLPEQQELFPVNEGILQFW
                     ISSEDQMYGMSENLKGNNINSRLVYIKEPITDLSLENIQVHLKSLDADNEDIPFSGAF
                     SIEFRLSKQTITCTDYKYDEDVLALWNKVNPSFALKSMFGGYDELMEPVCNTFTAKEP
                     FNQLGGYPYFDQIDPRTNDQELKMYDRVLLQIDSTRDGNSSIIWGDLGIANILVKSTD
                     LEAMKFDDYMYSWDCS"
     gene            8490..8876
                     /locus_tag="NBI57_001246"
     CDS             8490..8876
                     /locus_tag="NBI57_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HCD1822202.1"
                     /translation="MAIVNKVIIVEGKSDKKRVQQVIAEPVNIICTHGTMSIDKLDDM
                     IESLYDKQVFVLADSDDEGDRIRNWFKRYLSESEHIFIDKTYCQVANCPKQYLAHVLS
                     KHGFTCKKETPLLPNINNERLVLVNE"
     gene            8869..9165
                     /locus_tag="NBI57_001247"
     CDS             8869..9165
                     /locus_tag="NBI57_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HCD1822203.1"
                     /translation="MNNSLDIKDVTTFYEEDKHLIFGYTPTCGTCKVSERMLDIANEI
                     LQLPLLKIDLNFYPQFCKDMQIMSTPILLLMNKDKEVKRIYAFKSVTDLLENLK"
     regulatory      9223..9326
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            9415..10440
                     /locus_tag="NBI57_001248"
     CDS             9415..10440
                     /locus_tag="NBI57_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HCD1822204.1"
                     /translation="MIELKEVVKEYRTKNKEVLAVDHVNLSIRAGSIYGVIGFSGAGK
                     STLIRMFNHLEAPTSGEVIIDGDHIGQLSKNGLRAKRQKVSMIFQHFNLLWSRTVLKN
                     IMFPLEIAGVPRRRAKQKALELVELVGLKGREKAYPSELSGGQKQRVGIARALANDPT
                     VLLCDEATSALDPQTTDEILDLLLKIREQQNLTIVLITHEMHVIRRICDEVAVMESGK
                     VIEHGPVTQVFENPQHTVTKRFVKEDLNDDFETSLTELEPLEKDAYIVRLVFAGSTTT
                     EPIVSSLSTAYDIKINILEANIKNTKNGTVGFLVLHIPYISSVDFGKFEKELIERQVK
                     MEVLRHG"
     gene            10433..11128
                     /locus_tag="NBI57_001249"
     CDS             10433..11128
                     /locus_tag="NBI57_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCD1822205.1"
                     /translation="MGKSFSEIINEMITMPNIQWPEVWTAIVETLYMTVVSTIFAFIL
                     GLILGVLLFLSAKGKSIGARLFYSIVSFIVNLFRAIPFIILILLLIPFTSLILGTISG
                     PTGALPALIIGAAPFYARLVEIAFKEIDKGVIEAAWSMGANTWIVIRKVLLPEAMPAL
                     VSGITVTAIALVGSTAVAGVIGASGLGNLAYLTGFTRNQNDVILVSTVFILIIVFIIQ
                     FIGDWITNKLDKR"
     gene            11146..11967
                     /locus_tag="NBI57_001250"
     CDS             11146..11967
                     /locus_tag="NBI57_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HCD1822206.1"
                     /translation="MKKLFGLILVLTFAVVLAACGNGNKSGSDDKKITVGASPAPHAE
                     ILEKAKPLLEKKGYDLDIKTINDYTTPNKLLDKGEIDANYFQHTPYLNTEKKDKGYKI
                     VSAGDVHLEPMAVYSKKYKSLKELPKGATVYVSNNPAEQGRFLKFFVDAGLIKIKKGV
                     KIEDAKFSDITENKKDIKFNNKQSAEFLPKIYQNEDADAVIINSNFAIEQKLNPKKDS
                     IAVESAKDNPYANLIAVKEGHQDDKKIKALIEVLQSKDIQDFINEKYNGAVIPAK"
BASE COUNT         4364 a         1725 c         2133 g         4034 t
ORIGIN      
        1 tagaatgcta aagtttatta attcaaacaa gtgagcatat atagggatgt atagtattaa
       61 tatgaaaaga atatttacat actaaaaact cttaaagttg tataattcgt taatattttt
      121 agaatcagat atcaaaaaaa tgtgcaaaat cacttttgca catttacaaa tactagtttt
      181 ctgataagat taataataac actttaaata gcgcttaata aatgaagggg gcaagtctta
      241 tgacgtttta caatttcatc atgggttttc aaaatgataa cacaccattt ggtatattgg
      301 ccgaacacgt tagtgaagat aaagcattcc ctcgattaga agaaagacac caagtaatta
      361 gagcatatgt gatgtctaat tacacagatc atcaattaat tgaaactaca aatagagcta
      421 ttagcttata tatggcaaat taatttgagt agtaccaatt atgatgtatt agtgcatccc
      481 aaatatcttt tgttttaaag tttatttcat catttcttat cgaaaatggt gtaataatgt
      541 ctttatctaa ccaagtgttg ataagttcat ttggtacacc atctaacaac atttcacttt
      601 tactaattat aaaacattcc cagtcaagtg aaacattttg tggattcaca taattacatt
      661 gattatgatg atccataaac actcactcct ttaaaattct gtactcttca ttgcgtttta
      721 ccccgtcaca ttatctttta aactaaaatc atcattactt atgaaaaaaa tgtacatcaa
      781 aagcaaaggt tttcgctacc gaaaaagttt aaataatggt ttaatatatt tggtactcat
      841 tttaataaaa agagaataca ttttgagcta tcaatacttt ttattgaaga ggtgttattg
      901 ttggctaaaa cgttatattt aatgcgccac ggacaaactt tgtttaattt taagggacta
      961 attcagggat ttggagattc gccgctaaca gaacttggaa ttgctcaagc tcaaaaggca
     1021 cgtagttatt atgaaactaa ggggataaac ttcgatttat atgcatcatc aacgcaagaa
     1081 cgcgcaagtg acacacttga aaatgttgca cctaaccaat cgtatcaacg ttttaaggga
     1141 ctgaaggaat ggcattttgg aatatttgaa ggtgagtcag tctatctatt tgataatcta
     1201 tacaagcctg aggacttatt cggagatcga attgttcctt tcaaaggaga ggcaaggcaa
     1261 caagttgaag aacgcattgt gaaaacttta catgacatta tgtctcaaac aaagaataat
     1321 gcattagtcg tgagtcatgg aacaataatg ggagtatttt taagatattg ccttaaacta
     1381 gatgaagcat taaagcataa tatcggtaat tgtaatatcc tgaaatttga atatgacaat
     1441 ggaacattta aatttgttga gttaattgat ccaaatttat aataaataaa gcgttataca
     1501 tagacacaag tatgtctttt gtataacgct tttaaaatac aactacataa cgctttagaa
     1561 taataattga ataagttttt gtaatatcat tagagcaaca ataataatta ttatgcttga
     1621 tattttattg attattgtta ataattttcc agttttatca attgatccaa ccatttttcc
     1681 taaaattgcg agtaaaaaga accataacca tgaaacgcta atacaagcga ttgtaaacgc
     1741 aattttattg ctgccactat ataatgcagc actactacca attacaccaa ttgtatctaa
     1801 aatagcatgt ggattgagta atgaaactga taaagcaaaa cttacttgtt tcattggaga
     1861 tataatttga gcttctccat ctgttgaggg tttatcatgc caaatggtcc aagccatata
     1921 catcaagaaa attaaaccaa ctatataaat aattgcttga agtacaggta aagacataat
     1981 aatgatagat actcctacca ctgcaataat aataagtaag ctgtctgaca acccggctgt
     2041 aattattgca ggcaatacat atctatattt tggttgatta gctccttggt taaaaataaa
     2101 tacattttgt gcacctaaag gtaaaattag accaatcgct aagataaatc catgaataat
     2161 tgcggttacc attaagtttt gacaccccta aaacatagaa tattctgaaa aaacttattg
     2221 ttatcatatc aaataaaaaa caactactca atagtagagt agttgtatga aaaatagaac
     2281 ctatatgatt tgaatttatg agaaatgtag aatatataaa actgtattac ttattaatca
     2341 aatcataaaa aagccaatgt tcatcgaaat aggtatttcc gattttaatt gcatttttct
     2401 caaaaccaag aatgtcgaat cctagtgcac tatagaaaac tttagcacct atgttgtttg
     2461 acacaattga tgtaagtaaa gattcgtaat tgttctgccg tgcataatta ataatgaaat
     2521 taatgagctc gctattaatc gatttatcgt tattagtaac aaagttgtat ttaattaatg
     2581 atttatgttc ttttccaacg tatcgaattt gttctaaggc agctgttgcg ataagtgttt
     2641 cgttttcata acagccaaat acaatacatt tcggtgaacc ttgatttaaa atgtcatgta
     2701 ttaaggtgtc agtcattgtt tgactataat gtgaatcttg agtgaattct tcattgactg
     2761 tcgaaacaag tctcttatac tcattaaaat cattattcgt taatgtttta atttcaatca
     2821 ttactgacct ccttaatcat tattaagaaa gattttgtac taaaaaaatt tcttaattac
     2881 atattaaaat gtttattaaa attttgcaat cactgattta tatgcacatt cttgtaaatg
     2941 gattatcaat ttaattatat ttatttttaa gttcaggaaa ttatggaatt ggttattgat
     3001 cgacactgga cttttatttg gagacgttat gcgaagaagt caagccagta ttggaaccgg
     3061 tcacatttat gatgactaag ggacaatcat tttcagttaa atattgccag aaatttcagt
     3121 cacgaataga aaaaataacc aaccaaaaat ttttggttgg caaatttgaa ttttatgtga
     3181 attttattaa taacgttgaa taatctcttt aagttaatgc taatctacaa catttacatt
     3241 taaatcgaca ttaatatttc cttgagtcgc ttttgaatat ggacaaaatt catgagccat
     3301 ttgtaaatat ttttcagctt cttcttgaga tataacattt ttaattgtcg catcaattga
     3361 aacacttaat ttaggacttt ctgagtctga atcatcttct agtctcactg ttagtgttac
     3421 ttctggatga gcatcacgca ctttgttttg ctttaaaatt aggtcgaaag caccgttgaa
     3481 gcaagatgca taacctgctg caaataattg ttcggggtta gtagctttac catctgcttg
     3541 agcaggcgga acgatatcaa tatctaacgc tcgatcatca gtataaacat gtcctttacg
     3601 tccgccaaca ttcgtcgctt tagtttcata atgtattgcc attcttataa cctcctaatt
     3661 taaaatacat ttatgcttta cccattcgaa aataccttaa tcattttcat ttatagcatt
     3721 gtttgattga aggataaaaa gttgttgttt acaataaaaa taatgagtat ctgaaatgag
     3781 ggatttacta tgacacatgt ggaagtagta gcgactatcg cgccacaatt atctatcgaa
     3841 gaaactttaa ttcaaaaaat taatcatcgt attgatgcaa tagacgtatt agaattacga
     3901 attgatcaaa ttgaaaatgt cacagttgat caagtggcag aaatgattac aaagctgaag
     3961 gttatgcaag attcattcaa attattagtt acgtatcgta caaagttaca aggtggctat
     4021 gggcaattta taaatgactt gtatcttaat ttaatatcag acttagcaaa tatcaatggc
     4081 atagatatga ttgatataga atggcaagca gatattgaca ttgaaaaaca tcaacgaatc
     4141 attaaacact tgcaacagta taataaagag gtggttatat cacatcataa tttcgaaagt
     4201 acgcctccat tagatgaatt gcaatttata ttttttaaaa tgcaaaaatt caacccagaa
     4261 tacgttaaat tagcagtaat gccacataat aaaaatgatg tgttaaattt attgcaggca
     4321 atgtctacat tttcagatac tatggactgc aaagttgttg gtatttcaat gtctaaactt
     4381 ggactaataa gtagaacggc tcaaggcgtt tttggtggtg cattgactta tggttgtatc
     4441 ggagaaccac aagctccagg acagattgat gttactgatt taaaagcaca agtgacttta
     4501 tactaataaa atgaattagg ttgcttttgt gtaagtgctg tcttataatt gagaagtatt
     4561 atcattgttc gtggaggagc gattttagat ggaattacaa caagcaatag ctaatagaag
     4621 aagtgtgaaa aaatttaaaa gagatatgca catagatgac gcattgctat atcaagcaat
     4681 tgagaaagct gctgatgctc caaatcacgg aatgagggaa ccatggagag ttgtgcatgt
     4741 tccgaaagac agattaggag atatgagtaa ggatatttct aaatttgcat ttcctaatga
     4801 attagataag caacaatgtc attatgatgc agtaacgaaa ctaggtggca tgttattgct
     4861 tattttaaaa acagatccaa gacaacgtca aaatgatgaa aactactttg catttggtgc
     4921 atatgcacaa aatcttatgt tgttacttta tgaagcgggg ataggtacat gttggaaatc
     4981 gccattatat atctatgatc ctaaagtaag aaaaacactt ggtataaaga aagatgaagt
     5041 tcttgctgga ttcttatatt taacggattt agaagaagat atgcctaaag caccacgtaa
     5101 aaatagaaac ttaattacat tatattaata tgtataatta tagaaaacat taataaaagc
     5161 tgagacatga attgatggac atctatcgag ttagagattt aatctaattt actagagtcg
     5221 gtacaatcac agtctcagct ttttattgtg aagtatatac acatttgtat tttagtattt
     5281 atttaaaagt ttctgctaaa aatgattcaa cttgttcagg tgacttagca tttgctgaat
     5341 gaaggtgtgc aattttgtcg ccgtttttaa atactagcaa gctagggata cccataactt
     5401 cattttcaac aactacatct tctaattcat cacgattaac agtataccat tggtaatcat
     5461 tatattgttc tacgattggg tcaatccata aatccatagc acgacagtct gggcaccatc
     5521 ctgcctcaaa tttaacaatt acaggtgtat cgctattaat tatagattta aatgattcat
     5581 tacttttgat tgcttgcatt gtaacaactc ctctagatag tttaataatt tttattatag
     5641 ctaaatttat atcataataa aaaattttag cttcaaaatg aaagcctttt gctatggaaa
     5701 atgatatatt tattttaaat acataaagga ggttgcagtc gcatgattaa attttaccaa
     5761 tataagaatt gtacaacttg taaaaaggca gcaaagtttt tagatgaata tggcgtaagt
     5821 tatgaaccaa ttgatatcgt tcaacataca cctacaataa atgaatttaa aacaataatt
     5881 gcaaatacag gcgtagaaat taataaattg tttaatacac acggtgcgaa atatcgtgag
     5941 cttgatttga aaaataaatt acaaacttta tcagatgatg aaaagttaga gttgttatca
     6001 tctgatggta tgttagtaaa gcgtcctcta gcagtaatgg gcgataagat aacattagga
     6061 tttaaagaag atcaatataa agagacttgg ttagcgtaag tgaaatgtaa acgtttacta
     6121 aatatctcga tatttagatt cattacatgt aaaatgaaat aagctataca attgttaatt
     6181 tttataaata tagttgaata gcatctagcc ttatggcatc attaatgatg taaagattaa
     6241 ttaggagggg attctcttgg cagtaccaaa tgaattgaaa tattcaaaag agcatgaatg
     6301 ggttaaagtt gaaggtaatg tagcaacaat tggaatcaca gaatacgcac aaagcgagtt
     6361 aggtgatatt gttttcgttg aattaccaga aacagatgat gaaattaatg aaggggatac
     6421 gtttggtagc gtagaatcag ttaaaactgt atcagaatta tatgcaccaa tctctggtaa
     6481 agtagttgaa gtcaacgaag aactagaaga tagtcccgaa tttgtaaatg aatctccata
     6541 cgaaaaagca tggatggtaa aagtagaaat tagtgatgaa agtcagcttg aagctttatt
     6601 aacagctgaa aaatattcag aaatgattgg tgaataatca ccgtgtaact ccttaatcta
     6661 agattgagga gtttattttt agtctgagta agtaaataat atgaaagaaa atttattagg
     6721 cactattatt tggagtattg ctacatttta ttattcaaga atgatgaaaa taatgaattt
     6781 agctatttta aaaataaaaa ttgggggaag ttaatatgct aaacattcaa gacgttagtc
     6841 atctttctaa aaaggagcaa aaagcatata accgtttcgt agaatctgta gaaaacggta
     6901 atttaccagt actaccatgt attgaaatgg atttaaaaga gatgcaagaa gaaactttaa
     6961 gccagagtaa gattggtgga atgccatttt taaaatcttt taaagatata ccattagatg
     7021 aaaataatgt accaatggta ttgttagcac agattaattt gaatgatctt ccagaacaac
     7081 aagaattatt tcctgtaaat gaagggatat tgcagttttg gattagttca gaagatcaaa
     7141 tgtatggtat gtctgaaaat ttaaagggaa acaatataaa ctcaaggctt gtttatataa
     7201 aagagccaat tacagattta tcactcgaaa atattcaagt gcatttgaag tcattagatg
     7261 ctgataatga ggatatcccg ttcagtggag cattttctat agaatttaga ttgtcgaaac
     7321 aaactattac atgtactgat tataagtacg atgaggacgt gcttgcattg tggaataaag
     7381 tcaatccatc cttcgcgcta aaatcgatgt ttggtggata tgatgaatta atggaacctg
     7441 tgtgtaacac atttactgct aaggagccat ttaatcaact tggtggttat ccatattttg
     7501 accaaataga tccaagaacg aacgatcaag aactgaaaat gtatgataga gtcttactgc
     7561 aaattgattc tacaagagat ggtaattctt cgattatatg gggtgattta ggtattgcca
     7621 atatcttagt gaaatctact gaccttgagg ctatgaagtt tgatgattac atgtattcat
     7681 gggattgcag ctaagagaat cataaaaata tattacttta ataagtgtag tatctataga
     7741 tcaatctagg gagtgggaca gaaatgatat tttcgcaaaa tttatttcgt cgtcccaccc
     7801 caacttgcat tgtctgtaga aattgagaat ccaatttctc tttgttgggg cccatcccca
     7861 acttgcattg cctgtagaat ttcttttcga aattctctgt gttggggccc ctgactagaa
     7921 ttgaaaaaag cttgttacaa gcgcattttc gttcagtcaa ctactgccaa tataacttcg
     7981 tagagcatag aacattgatt tatgtcccag cctgttttca taagccacag ctaatgtgta
     8041 tttaaaaata ggaatacatg agtaaaactc atgcataaga aatactaatt tctaaagaaa
     8101 aagtatttct ttatgttggg ccccccaact tgcacattat tgtaagctga ctttccgcca
     8161 gcttctgtgt tggggccccg ccaacttgca cattattgta agctgactta ccatcagctt
     8221 ctgtgttggg gccccgctat aattgaaaag tttgttatag gtgtattttc ttttggttaa
     8281 ctattgttaa tataacattg tagattttag gatgttgatt tttgacttag cctttgtctg
     8341 cttatgttaa atgttccagc tagtcaaaca ttgagtttgt aattattata ttaagctagt
     8401 ttaaattttt tgtgatttat gttttattca taccaatttt gtgaaatgat acagtgacag
     8461 atgtttattg attttaaagg gtgatgatga tggctattgt aaataaagtg ataattgttg
     8521 aaggaaaatc tgataaaaaa agggtgcaac aggttattgc agaaccagtc aatattattt
     8581 gtactcatgg aacaatgagt atagataagc ttgatgatat gatagaatca ctgtatgata
     8641 aacaagtttt tgtattagcc gattctgatg acgaaggaga tcgaattaga aattggttta
     8701 aacgttattt gagtgaaagt gaacatatat ttattgataa aacttactgt caagttgcga
     8761 attgccccaa acaatatttg gcgcatgtac tttcaaaaca tggctttact tgtaagaaag
     8821 aaacacctct tttaccgaat ataaataatg aaaggttagt tttagtaaat gaataattca
     8881 ttagacatca aagatgtaac tacattttat gaggaagaca aacatttaat atttggttat
     8941 acaccaacgt gtggtacttg taaggtttca gaaagaatgt tagacattgc taatgaaata
     9001 ttgcagttac cattattgaa aatagattta aacttttatc ctcagttttg taaagatatg
     9061 caaatcatgt ctacgccgat tttattgttg atgaataaag ataaagaagt aaaacgaatt
     9121 tatgcattta aatcggtgac tgatttgtta gaaaatttaa aatagttatt gacgaaattt
     9181 tactaaggtg ttaggattaa tacaaattaa atgatgcgta aactcttatc gagagtggtg
     9241 gagggatgtg ccctacgaag cccggcaacc gtcttatata gaaatggtgc caattcacat
     9301 aaagttttaa cttttgaaga tgagagaaac aatactacta ttgctttctc aatttttcta
     9361 tcgatattga gaaagcattt tttattttat taagcaacac agggaggaat caacgtgatt
     9421 gaattaaaag aagttgttaa agaatatcgg actaaaaata aagaagtcct tgctgtagat
     9481 cacgttaatt tatcgattcg agcaggatcg atttatggcg tcattggttt ttctggagca
     9541 ggaaaaagta ctttgattcg aatgtttaat catttagaag cgcctacatc aggtgaagtt
     9601 attatagatg gagaccatat aggtcaattg tccaaaaatg gattaagagc aaaaagacaa
     9661 aaagtaagta tgatcttcca acattttaat ttgttatggt caaggactgt gttaaaaaat
     9721 attatgtttc cgcttgaaat tgcaggtgtc cctagaagga gagctaagca aaaagcatta
     9781 gaacttgtcg aactcgtcgg tttaaaaggt agagaaaagg cttatccatc agagttatca
     9841 ggtggacaaa agcaacgtgt tgggattgca cgagcgttag ctaatgatcc aacggtcttg
     9901 ctttgtgatg aggcaacaag tgcacttgat ccgcaaacaa cagatgaaat tttagatcta
     9961 ctactaaaaa ttagagaaca acaaaattta acaattgtac taattacgca tgaaatgcat
    10021 gtcattcgtc gtatttgtga tgaagttgca gttatggaaa gtggtaaagt gatagaacat
    10081 ggaccggtga cacaggtttt tgaaaatccg caacacactg tgacaaaacg atttgtgaaa
    10141 gaagatttaa atgatgattt cgaaacatct ttaacagaat tagagccatt agaaaaagat
    10201 gcttatatcg ttagattagt tttcgctggt tcaacaacaa ccgagcctat tgtatcgagt
    10261 ctatcaactg cctatgatat taaaattaat attttagaag caaatattaa aaatacaaaa
    10321 aatggaacag tcggcttttt agttctgcat attccatata tttcaagtgt agatttcgga
    10381 aaattcgaaa aagagttaat tgagcgacaa gttaaaatgg aggtgttaag acatgggtaa
    10441 atcatttagt gaaattataa atgaaatgat tacaatgcct aatattcagt ggccagaagt
    10501 ttggactgca atagtcgaaa cactatacat gacagtcgtc tcaactatat ttgcatttat
    10561 actcggtctt attttaggtg tgttattatt cttgtctgct aaaggtaagt ctatcggtgc
    10621 aaggttattt tattctatcg tttctttcat tgttaactta tttagagcga taccatttat
    10681 tattttaatt ttattattaa ttccatttac aagtttgata cttggaacga taagtggtcc
    10741 gacaggtgcg ttaccagcct tgattattgg cgcagcaccg ttttatgcaa ggctcgtaga
    10801 aattgctttt aaagaaattg ataaaggtgt catcgaagcg gcctggtcaa tgggtgccaa
    10861 tacttggata gtaattcgta aagtcctctt acctgaagcc atgccagcgc tagtgtctgg
    10921 cattacagtt acagcaatcg ctttagttgg ttcaacagca gttgcaggtg taattggtgc
    10981 cagtggttta ggaaatttag catacttaac aggtttcact cgaaatcaaa atgatgtcat
    11041 tttagtatca actgttttta ttttaattat tgtatttata atccaattca ttggggattg
    11101 gattacaaat aaacttgata aacgataaat tggggggttc attttatgaa aaaattattt
    11161 ggtcttattt tagtattaac atttgcagtt gtattagcag cttgcggtaa tggaaacaaa
    11221 agtggcagtg acgataagaa aataacagta ggtgcttcac cagcaccaca tgctgaaatt
    11281 ttagaaaaag caaaaccatt attagagaaa aaaggttatg acctagatat taaaacaatt
    11341 aacgattaca ctacgcctaa taaattacta gataaaggtg aaattgacgc aaactatttc
    11401 caacatacac catatttaaa cacagagaaa aaggataaag gttacaaaat cgtaagtgcc
    11461 ggtgatgttc acttagaacc tatggctgta tactctaaaa agtataaaag tttaaaagaa
    11521 ttaccaaaag gtgcgacagt ttatgtgtct aataatccag ctgaacaagg acgcttctta
    11581 aaattcttcg ttgatgcagg tttaattaag atcaaaaaag gcgtaaaaat tgaagatgct
    11641 aagtttagtg atattacaga gaataaaaaa gatattaagt ttaataataa acaatcagca
    11701 gaattcttac ctaaaattta tcaaaatgaa gacgctgatg ctgttatcat taattcaaac
    11761 tttgcaatcg aacaaaaact aaatcctaaa aaagattcta ttgctgtaga aagtgcgaaa
    11821 gataatcctt atgcaaactt aattgctgtt aaagaaggac atcaagatga taagaaaatc
    11881 aaagcattaa ttgaagtatt acaatctaaa gatattcaag acttcattaa tgaaaaatac
    11941 aacggtgcag ttattcctgc taaataaatc atattaatgg ctcagtaacg tgatgttgct
    12001 gggcttttta atttaaacag gtatttatat gatatttagg aatgagatgc tattacggat
    12061 aaagtaaaat cctatatgca tgcgtgctac tcatttattc ttatttttaa tttcacatgg
    12121 atagtgacta ctacatcata aatcattagt gattctttat tatttcagtc ccactccctc
    12181 aagttaatga tgaatctatt tcgctagtta taaaatgacg tcaaatcaat atcaaaaatt
    12241 atttaagtaa aatgtt
//
LOCUS       DAHHSC010000058        12180 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.058, whole genome shotgun sequence.
ACCESSION   DAHHSC010000058 DAHHSC010000000
VERSION     DAHHSC010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 12180)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12180)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12180
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.058"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            complement(<1..714)
                     /locus_tag="NBI57_001251"
     CDS             complement(<1..714)
                     /locus_tag="NBI57_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HCD1822207.1"
                     /translation="MDIVNIVIMLLIGVFGGFISGLVGVGGAIIIYPAILLLPPLFGA
                     PAYSAYIASGLTSSQVFFSTLSGSLKARKKTEFSPQLVLYMGGGMIIGSMLGAFLANL
                     FDATFVNTVYIIIALLALTLMFIKVKPSSEKSSFNKYLLVIIGLFIGIISGIVGAGGA
                     FIIIPILLVLFKLPMNTVVANSIVIAFISSIGAFVIKLIQGYIPLYDALFLIIGSIIF
                     APIGLKLSKKVPNIIQKWII"
     gene            complement(714..974)
                     /gene="cstR"
                     /locus_tag="NBI57_001252"
     CDS             complement(714..974)
                     /gene="cstR"
                     /locus_tag="NBI57_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="persulfide-sensing transcriptional repressor
                     CstR"
                     /protein_id="HCD1822208.1"
                     /translation="MNYDKKMINRINRIQGQLNGIIKMMEEGKDCKDVITQISASKSS
                     LQRLMGIIISENLIECVKVAADDEESSQELINEAVNLLVKSK"
     gene            1111..2175
                     /gene="cstA"
                     /locus_tag="NBI57_001253"
     CDS             1111..2175
                     /gene="cstA"
                     /locus_tag="NBI57_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001837745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="persulfide response sulfurtransferase CstA"
                     /protein_id="HCD1822209.1"
                     /translation="MKQYGEKFIDEFSKAELEKLAKQGQLIDVRTEEEYALGHINGSI
                     LHPVDEIESFNKEKNKTYYVICRSGNRSANASKYLAKQGYNVINLDGGYKAYEENDSY
                     DTQEEYKSIEIKADRKQFNYRGFQCPGPIVKISQEMKNIEVGDQIEVKVTDPGFPSDI
                     KSWVKQTRHTLVKLDENNNGINAIIQKEKAKDLDINYSAKGTTIVLFSGELDKAVAAL
                     IIANGARAAGKDVTIFFTFWGLNALKKVQTVNVKKQGIAKMFDLMLPQKNIRMPLSKM
                     NMFGLGNMMMRYVMKKKNVDSLPTLINQAIEQNIKLIACTMSMDVMGIQKEELRDEVE
                     YGGVGTYIGATENANHNLFI"
     gene            2206..3540
                     /gene="cstB"
                     /locus_tag="NBI57_001254"
     CDS             2206..3540
                     /gene="cstB"
                     /locus_tag="NBI57_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="persulfide dioxygenase-sulfurtransferase CstB"
                     /protein_id="HCD1822210.1"
                     /translation="MFFKQFYDNHLSQASYLVGCQRTGEAIIIDPVRDLSKYIEVADS
                     EGLTITQATETHIHADFASGIRDVAKRLNANIYVSGEGEDALGYKNMPSKTQFVKHGD
                     IIQVGNVKLEVLHTPGHTPESISFLLTDLGGGSSVPMGLFSGDFIFVGDIGRPDLLEK
                     SVQIKGSTEISAKQMYESVQNIKNLPDYVQIWPGHGAGSPCGKALGAIPISTIGYEKI
                     NNWAFNEIDETKFIESLTSNQPAPPHHFAQMKQVNQFGMNLYQSYDVYPSLDNKRVAF
                     DLRSKEAFHGGHTKGTINIPYNKNFINQIGWYLDFEKDIDLIGDKSTVEKAKHTLQLI
                     GFDKVAGYLLPKSGISTQSVHSADMTGKEEHVLDVRNDEEWNNGHLDQAVNIPHGKLL
                     NENIPFNKEDKIYVHCQSGVRSSIAVGILESKGFENVVNIREGYQDFPESLK"
     gene            3559..4752
                     /gene="sqr"
                     /locus_tag="NBI57_001255"
     CDS             3559..4752
                     /gene="sqr"
                     /locus_tag="NBI57_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II sulfide:quinone oxidoreductase Sqr"
                     /protein_id="HCD1822211.1"
                     /translation="MNKHYQIVIIGGGTAGVTVASRLLRKNQNLKEKIAIIDPADHHY
                     YQPLWTLVGAGVSSLKSSRKDMESVIPEGANWIKQAVSSFQPENNSVILGDNTVVYYD
                     FLVVAPGLQINWSSIKGLKENIGKNGVCSNYSPDYVNETWNQISNFKQGNAIFTHPNT
                     PIKCGGAPMKIMYLAEDYFRKHKIRSNANVIYATPKDALFDVGKYNKELERIVEERNI
                     TVNYNYNLVEIDGDKKVATFEHIKAYDRKTISYDMLHVTPPMGPLDVVKESTLSDSEG
                     WVDVNPTTLQHKSYSNVFALGDASNVPTSKTGAAIRKQAPIVANNLLQVMNNQMLTHH
                     YDGYTSCPIVTGYNRLILAEFDYNKNTKETMPFNQAKERRSMYIFKKDLLPKMYWYGM
                     LKGLI"
     gene            complement(5419..6405)
                     /locus_tag="NBI57_001256"
     CDS             complement(5419..6405)
                     /locus_tag="NBI57_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000662030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HCD1822212.1"
                     /translation="MKENFWSELPRPFFILAPMEDVTDIVFRHVVSEAARPDVFFTEF
                     TNTESFYHPEGIHSVRGRLTFSEDEHPMVAHIWGDKPEQFRETSIQLAKMGFKGIDLN
                     MGCPVANVAKKGKGSGLILRPDVAAEIIQATKAGGLPVSVKTRLGYYEIDEWKDWLKH
                     VFEQDIANLSIHLRTRKEMSKVDAHWELIEAIKNLRDEIAPNTLLTINGDIPDRKTGL
                     ELAEKYGIDGVMIGRGIFHNPFAFEKEPREHTSKELLDLLRLHLSLFNKYEKDEIRQF
                     KSLRRFFKIYVRGIRGASELRHQLMNTQSIAEARALLDEFEAQMDEDVKIEL"
     gene            6714..6917
                     /locus_tag="NBI57_001257"
     CDS             6714..6917
                     /locus_tag="NBI57_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1822213.1"
                     /translation="MEDLKESLKSLGWWDLFFAIPMFLLFAYLPNYNFITIFLNIVII
                     IFFSIGLILTTHIIIDKIKSNTK"
     gene            6966..7262
                     /locus_tag="NBI57_001258"
     CDS             6966..7262
                     /locus_tag="NBI57_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000179892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TfoX/Sxy family protein"
                     /protein_id="HCD1822214.1"
                     /translation="MATKKDVHDLFLNHVNSNAVKTRKMMGEYIIYYDSVVIGGLYDN
                     RLLVKATKSVRHQLQDNTLVSPYPGAKEMILIPDITEAINLTNLFELIKNDLKN"
     gene            7470..8072
                     /locus_tag="NBI57_001259"
     CDS             7470..8072
                     /locus_tag="NBI57_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1822215.1"
                     /translation="MLISLVIPVFALLVIGGIIWVIIEGIVHISKKNKAIDNFLNQVN
                     KVSETYKFATAFLFLILATAGISQFYLYYIVSAFLFWLVILTFGIAGIIFLMPYGLCF
                     LPLYKQKKKKQTFKKYMVYTTIGLSICLGLSLVLVHTTKIYMDEGGVRYYYGSFVMKQ
                     AGGYAYLALAVLSTLLIVAKKATNKNKEIETVDNTNITER"
     gene            8339..11491
                     /locus_tag="NBI57_001260"
     CDS             8339..11491
                     /locus_tag="NBI57_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA2/NAM7 family helicase"
                     /protein_id="HCD1822216.1"
                     /translation="MSQLLNDTLSAWLLIESLSPGEVNFTAEDILSAEHFKNGAKQAQ
                     LQSFDEYFEIWNSERFIISEEKSETGELIFKFYRHCFRYNEINLKIQDIFDDYSDIHN
                     PNGTHCYGYTFNTDKHGKVIVDSIHIPMIMSALKEIEKNKNANIEEKFNDSVEKFFQK
                     VKEILADEPINEFKLKKMDKAYDEYFSVLNSKKDGLFGHYVAIEYVKDSDLPQPEFNS
                     FFISDIEKARKSPNQTLIDYIEGVEESQRIEVDENKEMFDKFLHPSRLPDGRWPSQTE
                     FRLSLMQQLAVNQITSGNERISSVNGPPGTGKTTLLKDIFAHLVVERGKELAKLNNPK
                     DAFVKTKIHETDDKYVYLLKESIAKYKMVVASSNNGAVENISKDLPKIEEIIRNPEKC
                     KFPKYEQNYANLAHELKDFAEIAEDLIGESAWGLFSGVFGKSTNINQVLSHMLKQDAN
                     DIGFAKLLQNENNRMSYNELMSEWQSHQRAFLEELRHVEMLKEESIRAYDVYKNCESF
                     SKIEQVINSEKTSIEEQVYHLDNETLRDNKEIEDLDNRINYIVKQIETLNELIKSIKE
                     SNKGFINKLKAMFNSEEDESYKDHNKEKQQLLTQQLELEKCKKNKHEDLVSKLKEKEK
                     LIKQLTKVQLQLDELNSQLQELEAYRIESKITIPEKDFWSDNNYDERQVTNLWTSDEL
                     QYRRAMLFLRAMILHKLLLIANNTTIYYAINDFKDRRKLIDANPDKVHNAWNVMHLIF
                     PVVSTTFASFKSMYGGIPKDFIDYLFIDEAGQAIPQAAVGALYRSKKVVAVGDPIQIE
                     PVVTLESHLIDNIRKNYHVPEYLVSKEASVQSVADNANQYGFWKSDATDSNQKTWIGI
                     PLWVHRRCLKPMFTIANQIAYNNKMVLPSNITKVGKTGWYDVKGNAVQKQFVKEHGEK
                     VVGLLADDWIEAIKEGKNEPSSFVISPFSAVQQQIKRMLKQQLPTRIDIERTKINQWV
                     DKSIGTVHTFQGKEAQKVYFVIGTDNTQDGAVNWSCEKPNLLNVAVTRAKKEFYVIGD
                     MQRIQMKPFYETIFKERNVK"
     gene            11548..12030
                     /locus_tag="NBI57_001261"
     CDS             11548..12030
                     /locus_tag="NBI57_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1822217.1"
                     /translation="MEKFNNWILNAISGSQTDKNETTEELKGAKFIILYAYSMLVLLA
                     LVISNIFIHILEPKLSITTQIIIVLILIEALIGLRFLKAYDVKRGKDKENKKNSKDFV
                     KLKSILVAILFTSLALTAGTVADIYGFTDLGNTRSDLIVWSIGGIIFGLVCYTMEDKK
                     "
BASE COUNT         4463 a         1703 c         2134 g         3880 t
ORIGIN      
        1 gataatccat ttttgtatta tgttagggac ttttttactt aattttaacc ctattggagc
       61 aaatataata ctccctatta taaggaataa ggcgtcatat aaagggatat aaccttgaat
      121 aagtttgatg acaaaagcac caattgaaga tataaaagca attactatac tattagcgac
      181 tacagtattc attggtaatt tgaataaaac caataatata ggaataataa tgaaggcacc
      241 acctgcacct actatacctg aaataatacc aatgaaaagg ccaatgataa ctaataaata
      301 tttattaaat gaagactttt cggaactagg tttcacttta ataaacatta atgttaatgc
      361 aagtaaagca ataatgatat ataccgtatt tacaaatgta gcatcaaata aatttgctag
      421 aaatgcacct aacatactcc ctataatcat gccgccaccc atataaagaa ctaattgtgg
      481 tgagaactct gttttttttc gagcttttaa tgagccactt aatgtactga aaaagacttg
      541 gctagaagta agacctgatg cgatatatgc gctatatgca ggggctccga ataatggtgg
      601 taataataaa atagctggat aaataatgat agcaccacct acgcctacta gaccagatat
      661 gaacccaccg aataccccaa tgagtaacat gataactata ttaacaatat ccattactta
      721 cttttcacca ataagtttac agcttcatta attaactctt gggagctttc ttcatcatcc
      781 gcagctactt ttacacattc tattaaattc tcactaataa tgatacccat caagcgttgg
      841 agtgaactct ttgatgcact tatttgtgta atgacatctt tacagtcttt tccttcctcc
      901 atcattttaa taattccatt tagttgccct tgtattctat taatacgatt aatcattttt
      961 ttatcataat tcatagtcat acctccactt ttaattgaat aaaaatatat taatagataa
     1021 acacaaatgt gtcaaatacc cctagaggta tttgacaagt tccattcaac cgtttagaat
     1081 acccctacag gtatttttag ggaggttatt atgaaacaat acggagaaaa gtttatcgat
     1141 gaatttagta aagcagaatt ggaaaaacta gccaagcaag ggcaattaat tgatgttaga
     1201 acagaagagg agtatgcatt aggacatatc aatggttcca tacttcatcc tgttgatgag
     1261 attgagtcat tcaataaaga aaaaaataaa acctattatg taatctgtag aagtggtaac
     1321 agaagtgcta atgctagtaa atatttagct aaacaaggtt ataacgttat aaatcttgat
     1381 ggtggttata aagcttatga agaaaacgat agttatgata cacaagaaga atataaaagt
     1441 atagaaatta aagcagatcg taaacaattt aactatcgtg gttttcaatg tccagggcca
     1501 attgtaaaaa ttagtcaaga aatgaagaat attgaagtag gtgaccaaat tgaagtcaaa
     1561 gtcacagacc ctggattccc tagtgatatt aaaagttggg tgaaacaaac aaggcatact
     1621 ttagttaagc ttgatgaaaa taacaatgga attaatgcga ttattcaaaa agaaaaagca
     1681 aaagatttag atataaatta ttctgctaaa ggtactacaa ttgtattatt tagtggagaa
     1741 ttagacaaag ctgtagcagc attgattatt gcaaatggcg ctagagctgc tggaaaagat
     1801 gtaactatct tctttacttt ttgggggctt aatgcattaa aaaaagtgca aacagttaat
     1861 gttaaaaagc aaggtattgc aaaaatgttt gatttaatgt tgccccaaaa gaatatacga
     1921 atgcctcttt ccaaaatgaa tatgtttggt ttaggaaata tgatgatgcg ctacgtaatg
     1981 aaaaagaaaa atgttgattc attaccaaca cttatcaatc aagctattga gcaaaatatc
     2041 aaattaatcg cttgtacgat gagtatggat gtcatgggta ttcagaaaga agaacttaga
     2101 gatgaagttg agtacggtgg tgtaggcact tatattggtg ctactgaaaa tgcgaatcat
     2161 aatttattta tctaattaaa tctattaata aaaggagttg ttatcatgtt ttttaaacag
     2221 ttttacgata atcatttatc tcaagcatca tatttagtgg gttgtcaacg tacaggagag
     2281 gcaataataa tagaccctgt tcgtgattta tcgaaatata tagaagttgc agattctgaa
     2341 ggtttaacaa ttacacaagc tacagaaaca catattcatg ctgattttgc ttcaggaatt
     2401 cgtgatgtgg ctaaacgctt aaatgcaaat atatatgtgt ctggcgaagg tgaagatgca
     2461 ttagggtata aaaatatgcc atcaaaaaca caatttgtta aacatggaga tatcattcaa
     2521 gtaggcaatg ttaaattaga agttctgcat actccaggac acacgcctga aagtattagc
     2581 tttttactca ctgatttagg tggtggttca agtgttccga tgggattatt tagtggtgac
     2641 tttatttttg ttggtgatat aggtagacct gatttattag aaaaatctgt tcaaataaag
     2701 ggttctacag aaattagcgc gaaacaaatg tatgagtccg ttcaaaatat taaaaattta
     2761 ccagactatg ttcaaatctg gccgggtcat ggtgctggaa gcccttgtgg taaagcatta
     2821 ggtgccatac ctatatctac aataggttat gagaaaatta ataactgggc atttaatgaa
     2881 attgatgaga ctaaatttat tgaatcatta acatcaaatc aaccagcacc accgcatcat
     2941 tttgcacaaa tgaaacaagt taatcagttt ggtatgaatt tatatcaatc atatgatgtt
     3001 tatcctagtt tagataataa gagagtagca tttgatcttc gtagcaaaga ggcctttcac
     3061 ggtggccaca caaaaggaac aatcaatata ccatacaaca aaaactttat taatcaaatt
     3121 ggttggtact tagattttga aaaagatata gatttaattg gagataaatc tactgttgag
     3181 aaagcgaaac acactttaca attaattggg tttgataagg tagcaggcta tcttttgcca
     3241 aaatcaggca tttcaaccca gtccgttcat agcgctgata tgacaggtaa agaagaacat
     3301 gtattagacg tacgtaatga tgaagagtgg aataatggac acttagatca agcagttaat
     3361 attccacatg gtaaattatt aaatgaaaat attcctttta ataaagagga taaaatatat
     3421 gtacattgtc agtcaggtgt tagaagttca attgcagtgg gtatattgga aagcaaaggt
     3481 tttgaaaatg tggtgaatat tagagaaggc tatcaagatt ttccagaatc attaaaataa
     3541 tttaaggatg tggaaaaaat gaataagcat tatcaaattg ttattattgg tggcggtaca
     3601 gcaggtgtta ccgtagcatc aagactatta agaaaaaatc aaaacttaaa agagaaaata
     3661 gcaattatag acccagcaga ccatcattac tatcaaccat tatggacgtt ggttggtgca
     3721 ggggtatcta gtttgaaaag ttctcgtaaa gatatggaaa gtgttatacc tgaaggtgct
     3781 aactggataa aacaggctgt ttcaagtttt caacctgaaa ataatagcgt tattttagga
     3841 gataatacag tcgtttatta tgatttttta gtagtagctc caggattaca gattaattgg
     3901 tcttcaatta aaggactaaa agaaaatata ggtaaaaatg gtgtttgctc taactattca
     3961 cctgactatg ttaacgaaac ttggaaccaa atttctaatt ttaaacaagg aaatgccatt
     4021 tttacgcatc caaacactcc tataaagtgt ggaggtgcgc ctatgaaaat tatgtattta
     4081 gctgaagatt attttaggaa acataaaatc cgttctaacg ctaatgtgat atatgcaacg
     4141 ccaaaagatg ctttatttga cgtaggaaaa tataataaag aattagaaag gattgttgaa
     4201 gaaagaaata taacagtcaa ttataattat aaccttgttg aaatcgacgg tgacaaaaaa
     4261 gtggctacat tcgaacatat caaagcatac gatagaaaaa caataagtta tgatatgtta
     4321 catgtaacac cacctatggg tcccttagat gtagtaaaag aaagtacact ttcagatagt
     4381 gagggttggg tagatgttaa cccaaccaca ttacagcata aaagctactc taatgtattt
     4441 gcacttggtg atgcttcaaa tgtacctact tcaaaaacag gcgcagctat tcgtaagcaa
     4501 gcacctatcg tcgctaataa tttattgcaa gtgatgaata atcaaatgtt aacgcatcat
     4561 tatgatggtt atacttcatg ccctattgtt actggatata ataggttaat acttgcagag
     4621 tttgattata ataaaaatac taaagaaaca atgccgttta atcaggccaa agaacgtaga
     4681 agtatgtata tatttaagaa agatttatta cctaaaatgt attggtacgg catgctaaaa
     4741 ggattaatat aataaagtac agaaaacaat aaatttttaa tgaaaaatct tttactataa
     4801 aagattaagt atttaaatga cgtgtcagtg ttgtgtttat atgtcgtgaa tttttagctc
     4861 taaatagtat aagattgaaa aagttgttac tgttttaaat gatcacgatg aagtcattca
     4921 ataagaatga ttatgaaaat agaaacagca gtaagatatt ttctaattga aaatcatctc
     4981 actgctgttt tttaaaggtt tatacctcat cctctaaatt atttaaaaat aattaatggt
     5041 atttgagcac gtttagcgac tttatgactg acattaccaa tttccatttc ttgccagata
     5101 ttcaaaccac gtgtactcaa aatgatagct tggtatgtac ctccaatagt aatttcaata
     5161 actttgtctg ttgaacacta agagcaattt taatttcata atgtgttgta aacatttttt
     5221 ttgattggag tttttttctg agttaaacga tatcctgatg tatttttaat tttgcaccat
     5281 ttccaaaagg ataagtgaca taagtaaaaa ggcatcatcg ggagttatcc tatcaggaaa
     5341 accaagataa tacctaagta gaaagtgttc aatccgtgtt aaattgggaa atatcatcca
     5401 taaactttat tactcatact ataattctat tttaacgtct tcgtccattt gggcttcaaa
     5461 ttcatctaga agtgctcgtg cttctgcaat tgattgtgtg ttcatcaatt gatggcgaag
     5521 ttcgctagcg cctcttatgc cacgcacata gattttaaag aatctacgca agctcttgaa
     5581 ttgtcgtatt tcatcttttt catatttgtt aaacaatgat aaatgcaatc tcaatagatc
     5641 taatagttcc ttgcttgtgt gttcgcgtgg ttctttttca aaagcgaatg gattatggaa
     5701 aatgcctcta ccaatcatga caccatcgat gccatatttt tctgccagtt caagtcctgt
     5761 ttttctatcg ggaatatcac cgttaattgt taacaatgta tttggtgcaa tttcgtcacg
     5821 taaattttta atagcttcga ttaattccca atgtgcatct actttactca tttctttacg
     5881 tgtacgaaga tgaatagata aattggcaat atcttgttcg aagacgtgct tcaaccaatc
     5941 tttccattca tcgatttcat agtagccaag gcgtgtttta acacttaccg gaagcccacc
     6001 tgctttagtt gcttgaataa tttcggcagc gacatccggt cttaagatta agccggaacc
     6061 cttacccttt ttagcaacat ttgctacagg acatcccata tttaagtcaa tgcctttaaa
     6121 gcccatttta gctagttgaa tactcgtttc acggaactgt tctggcttat ctccccatat
     6181 atgagcgacc atcgggtgtt catcttcgct aaaagttaag cgtccgcgca cactatgtat
     6241 gccttcaggg tggtaaaagc tttcagtatt tgtaaattca gtgaaaaaca catccggtct
     6301 agctgcttca cttacaacgt gtcgaaagac gatatctgta acgtcttcca ttggcgccaa
     6361 aataaaaaat ggacgtggta attcactcca aaaattttct ttcataatat atttataccc
     6421 tctttataat tagtatctcg attttttatg catgatgata ttaccacaaa agcctaactt
     6481 atacaaaagg aatttcaata gttgcaacca tttgaaaggg aagtctaaga gtagtctaaa
     6541 ataaatgttg tggtaagttg atcaatacaa agatcaagga ttatagtatt aaattgttca
     6601 ttattaatga tacactactt atgaatatga ttcagaattt tctttggcta cttttacagt
     6661 aaagcgattt tttagttatc ttataacaaa gacaaattta taaaggtgat attatggaag
     6721 atttaaagga atctttaaaa agtttaggtt ggtgggattt attttttgcg atacctatgt
     6781 ttctgctatt cgcatacctt ccaaactata attttataac gatatttctt aacattgtta
     6841 tcattatttt cttttccata ggtttgattt taactacgca tataattata gataaaatta
     6901 agagcaacac gaaatgaatc attaatacga atgtgattaa acataaaact gaaggagcga
     6961 ttacaatggc gactaagaaa gatgtacatg atttattttt aaatcatgtg aattcaaacg
     7021 cggttaagac aagaaagatg atgggagaat atattattta ttatgatagt gtggttatag
     7081 gtggtttgta tgataataga ttattggtta aggcgactaa aagtgtacgt catcaattgc
     7141 aagataatac attagtttcg ccatatccag gtgccaaaga aatgatatta attccagaca
     7201 ttaccgaagc aataaatctc actaatttat ttgagctcat aaaaaatgat ttgaaaaact
     7261 aatcataaat tgccatactc aactattttg aaaagtatca atttcatatt atcaaattat
     7321 tgagtatctc gatttattgt tatacacaaa cagtattttt gctatgatag agttaataaa
     7381 tataataaat tgaataagta actttacaaa actagaatga ttcaattcaa tcacaattat
     7441 ttgaaaatag aggtgagcag gtgaacgata tgttaattag tctcgtaatc ccagtttttg
     7501 ctttgttagt tattggtggt attatttggg tgattataga aggtatagta catatttcaa
     7561 aaaagaataa agcaatcgat aactttttaa accaagttaa taaagtaagt gagacatata
     7621 aattcgctac tgctttttta tttctaatct tggctacggc tggtatttct caattttatc
     7681 tatattatat agtatcagcg tttctttttt ggcttgtcat acttaccttt ggtattgcag
     7741 gtatcatttt tttaatgcca tatggattat gtttcctacc actttataag caaaaaaaga
     7801 aaaaacagac atttaaaaaa tacatggttt acactacgat tggtttgtca atttgtctag
     7861 gcttatctct agttttggtt cacactacga aaatttatat ggacgaaggt ggcgtaagat
     7921 actattacgg tagttttgta atgaaacaag cgggcggtta tgcttattta gctttagcgg
     7981 tactttcaac gttgttaatt gttgcgaaaa aagctacaaa taaaaataaa gaaatcgaaa
     8041 ccgtcgacaa tacaaatata acggaaagat aattaaggga gtgctcattc aggagtgctc
     8101 ttttttgatg tccaaattta gttgcaaatg aaggcataga agaaattttt atagtcattt
     8161 attggataat aaattatggt catatcgatt aattttatta gtgaggattt tacaaacatt
     8221 tgttgaataa atatggtaat gataatatat gaatattttt gaaaaaacac taatatgatt
     8281 aaattatttg ttaaaatagc attaagtaaa aaacaaatag ggaacaaggt gggatttcat
     8341 gagtcaattg ctaaatgata cgttatcggc ttggttgtta attgaatctt taagtccagg
     8401 agaagtaaat tttacagcgg aagatatact ctcagctgaa cattttaaaa atggtgcaaa
     8461 gcaagcacaa cttcaaagtt ttgatgaata ttttgaaata tggaattctg aacgctttat
     8521 tatatcagaa gaaaaatcag agactgggga acttatattt aaattttata gacattgctt
     8581 ccgctataat gaaattaatt tgaaaattca ggatattttt gatgattatt ctgatattca
     8641 taatccaaat gggacacact gttatggtta cacatttaac acagataaac acggcaaagt
     8701 gatagttgat tctatacata ttccgatgat tatgagtgca ttaaaagaaa ttgaaaagaa
     8761 caaaaatgcc aatatagaag aaaaatttaa tgattctgtt gaaaaatttt ttcaaaaagt
     8821 aaaagaaatt ttagcagatg aaccaattaa tgaatttaaa ttgaagaaga tggacaaagc
     8881 ttatgatgag tacttttctg tattaaattc aaagaaagat ggattatttg gacattatgt
     8941 agcaatagaa tatgtgaaag atagtgattt accacagccg gaatttaaca gtttcttcat
     9001 aagtgatatt gagaaagcaa gaaaatctcc caaccaaact ttaattgatt acattgaagg
     9061 tgtagaagaa agtcagcgca tagaagtaga tgaaaataaa gaaatgtttg acaaattttt
     9121 acatccttca cgtttgcctg atggacgatg gccatcacag actgagttta gattgtcttt
     9181 aatgcaacaa cttgctgtaa accaaattac gagtggtaat gaaagaataa gttcagttaa
     9241 tgggccacca gggacaggta agactacttt attaaaagat atatttgctc atctagtagt
     9301 tgaaagaggt aaagagttag ctaaactaaa taatcctaaa gatgcatttg tcaaaacaaa
     9361 aattcatgaa acggatgata aatacgtata cttactaaag gaatctattg ccaaatataa
     9421 gatggtagtc gcatctagta ataatggagc tgttgaaaat atatctaaag atttaccgaa
     9481 aattgaagaa attataagaa atcccgaaaa atgtaaattc cctaaatatg aacagaatta
     9541 tgcaaattta gcacatgaat taaaagattt tgctgaaata gctgaagatt tgattggtga
     9601 aagtgcctgg ggcttatttt ctggagtttt tggtaaaagt actaatatta accaagtatt
     9661 gagtcatatg ttaaaacaag atgcgaatga tattggcttt gctaaattac tacaaaatga
     9721 gaataatcgt atgagttata acgagttaat gagtgaatgg caatcacatc aacgtgcatt
     9781 tttagaagag ttgaggcatg ttgaaatgtt aaaagaagaa tctattagag catatgatgt
     9841 ttataaaaat tgtgagtctt tctctaagat tgaacaggtt attaatagtg aaaaaacaag
     9901 tattgaagaa caggtatatc atttagataa tgaaacgtta cgagacaata aagaaataga
     9961 agatttggat aatcgaatta attatattgt taagcaaata gaaactttaa atgagttaat
    10021 taaatccatc aaagaaagca acaaaggttt tattaacaaa ctgaaagcga tgtttaattc
    10081 agaagaagat gaaagctata aagatcataa taaagagaag caacaattat taacacaaca
    10141 gttagagtta gagaaatgta aaaaaaacaa acatgaagac cttgttagca aactaaaaga
    10201 aaaagagaaa ttaattaaac aattaactaa agtacagttg caattagacg agttaaattc
    10261 acagttacaa gagttagaag catatcgtat tgagtcaaaa attacaattc cagaaaaaga
    10321 tttttggagt gacaacaatt atgatgagcg ccaagttact aatctgtgga cgagtgacga
    10381 acttcaatac agacgtgcca tgctcttttt aagagcaatg atattgcata aattattatt
    10441 gattgctaat aatacaacta tttattatgc gattaatgat tttaaagata gaaggaaatt
    10501 aattgatgca aatccagata aagtacacaa cgcatggaat gtgatgcatt taatatttcc
    10561 agtagttagt acgacgtttg caagctttaa atctatgtat gggggcatac caaaagattt
    10621 catagactac ttatttattg atgaagcagg acaagcaata cctcaagcag ctgtgggagc
    10681 attatatcgt tcaaaaaaag ttgtagctgt aggtgatccg attcaaatag aaccggttgt
    10741 gactttagaa agtcatttaa ttgataacat tcgtaaaaat tatcatgttc cggaatatct
    10801 agtttctaaa gaagcttctg tgcagtctgt tgcagacaac gccaatcaat atggtttttg
    10861 gaaatctgat gctactgata gtaatcaaaa aacctggata ggcatacctt tatgggtgca
    10921 cagacgatgt ttaaaaccta tgttcacgat agctaaccaa atcgcttata ataataaaat
    10981 ggtgttgcca agtaatatta caaaagtagg taaaacaggt tggtatgacg ttaaaggaaa
    11041 cgcagttcaa aaacaatttg tgaaagagca tggtgaaaaa gtagtgggat tattagctga
    11101 tgattggatt gaagcaatta aggaaggtaa aaatgaaccg agctcatttg taatatcgcc
    11161 tttttcagca gtacagcaac agattaaacg tatgttaaag caacaactac cgactagaat
    11221 tgatattgaa cgtacaaaaa ttaatcaatg ggtcgataaa tccattggta ctgttcatac
    11281 ttttcaaggt aaagaggctc agaaggtgta ttttgtaata ggtactgata atacccaaga
    11341 tggtgctgtg aactggtcat gcgaaaaacc aaacttgtta aacgttgcag tgacaagagc
    11401 taagaaagag ttttatgtaa ttggcgacat gcaaagaata cagatgaaac cattttatga
    11461 gacgattttt aaagaaagaa atgtaaaata acatacaaaa aagtatatag aggaagttat
    11521 acattttaaa aggagcaaaa ttgaataatg gagaaattta acaactggat attaaatgca
    11581 ataagtggat ctcaaacaga caagaatgaa acaactgaag aattaaaagg ggcaaaattt
    11641 atcattttat atgcatattc aatgctcgtt ttgcttgcgt tagtaatttc taacatattc
    11701 attcacattt tggagcctaa actatcaatc accactcaaa tcatcatcgt tttgatttta
    11761 attgaagcac taattggact gcgtttcttg aaagcgtacg atgttaagcg tggcaaagat
    11821 aaagaaaata agaaaaatag taaggatttc gttaaactaa aatcaatttt agtagcaatt
    11881 ttatttacat cattggcgct gacagcaggt actgtagctg atatatacgg tttcactgac
    11941 ttaggaaata ctagaagtga tttaatcgtt tggagcatag gtggtattat atttggcctc
    12001 gtatgttaca caatggaaga taaaaaataa cgataaggag ctggcgatta taaagctagc
    12061 tcctttttta acttatatat gtaaagcact atcctaaggg tctttaatca tatgtcaata
    12121 attcctataa ttaatactct taaatattgt ttaaataagt tttaaaattt tacacatatt
//
LOCUS       DAHHSC010000059        12126 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.059, whole genome shotgun sequence.
ACCESSION   DAHHSC010000059 DAHHSC010000000
VERSION     DAHHSC010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 12126)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12126)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12126
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.059"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            complement(99..1172)
                     /locus_tag="NBI57_001262"
     CDS             complement(99..1172)
                     /locus_tag="NBI57_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017328539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HCD1822218.1"
                     /translation="MNIVKLMVIIIYLIIGSALGIIIIPEIANDLGLQNSSFLKNHYV
                     DGIIGSIFMFLIFGVFIRRVTNAIKGLEHFIMRRSAVEILFATIGLIIGLLISVMVSF
                     ILESIGNSIFNHFIPVIITILLCYFGFQFGLKKRDEMLMFLPENIARSMSQHTKSATP
                     KIIDTSAIIDGRILEVIRCGFIDGNILIPQGVINELQIVADSNDSVKREKGKRGLDIL
                     NELYDLDYPTKVIHPTKTHSDIDTMLLKLAKQYHASIITTDFNLNKVCHVHGIKALNV
                     NDLSEAIKPNVHQGDQLHILLTKMGKEPGQAVGYLDDGTMVVVDNAKNLIGSHVNLEV
                     VSLLQTSSGRIVFAKKIEDTVSL"
     gene            complement(1197..2561)
                     /gene="radA"
                     /locus_tag="NBI57_001263"
     CDS             complement(1197..2561)
                     /gene="radA"
                     /locus_tag="NBI57_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HCD1822219.1"
                     /translation="MAKKKVIFECMACGYQSPKWMGKCPNCGAWNQMEEIVEKAANPK
                     HGVKTKELAGKVQKLNSIKHETTPRVLTDSAEFNRVLGGGIVSGSLVLIGGDPGIGKS
                     TLLLQICASLSQKKKVLYITGEESLSQTKLRAERLDEDSSELQVLAETDLEVIYQTVK
                     EEQPDLLVVDSIQTIYHPEISSAPGSVSQVRESTQSLMNIAKQMNIATFIVGHVTKEG
                     QIAGPRLLEHMVDTVLYFEGDEHHAYRILRAVKNRFGSTNEMGIFEMKQSGLKGVNNP
                     SEMFLEERSTNVPGSTIVATMEGTRPLLIEVQALVTPTTFNNPRRMATGIDHNRLSLL
                     MAVLEKKENYLLQQQDAYIKVAGGVKLTEPAVDLSVIVATASSFKDKAVDGLDCYIGE
                     VGLTGEVRRVSRIEQRVQEAAKLGFKRVIIPKNNIGGWTYPEGIQVIGVTTVHEALSF
                     ALHS"
     gene            complement(3046..5502)
                     /locus_tag="NBI57_001264"
     CDS             complement(3046..5502)
                     /locus_tag="NBI57_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HCD1822220.1"
                     /translation="MLFGRLTERAQRVLAHAQEEAIRLNHSNIGTEHLLLGLMKEPEG
                     IAAKVLESFNITEDKVIEEVEKLIGHGQDHVGTLHYTPRAKKVIELSMDEARKLHHNF
                     VGTEHILLGLIRENEGVAARVFANLDLNITKARAQVVKALGNPEMSNKNAQASKSNNT
                     PTLDSLARDLTVIAKDGTLDPVIGRDKEITRVIEVLSRRTKNNPVLIGEPGVGKTAIA
                     EGLAQAIVNNEVPETLKDKRVMSLDMGTVVAGTKYRGEFEERLKKVMEEIQQAGNVIL
                     FIDELHTLVGAGGAEGAIDASNILKPALARGELQCIGATTLDEYRKNIEKDAALERRF
                     QPVQVDEPSVVDTVAILKGLRDRYEAHHRINISDEAIEAAVKLSNRYVSDRFLPDKAI
                     DLIDEASSKVRLKSHTTPNNLKEIEQEIEKVKNEKDAAVHAQEFENAANLRDKQTKLE
                     KQYEEAKNEWKNAQNGMSTSLSEEDIAEVIAGWTGIPLTKINETESEKLLSLEDTLHE
                     RVIGQKDAVNSISKAVRRARAGLKDPKRPIGSFIFLGPTGVGKTELARALAESMFGDD
                     DAMIRVDMSEFMEKHAVSRLVGAPPGYVGHDDGGQLTEKVRRKPYSVILFDEIEKAHP
                     DVFNILLQVLDDGHLTDTKGRTVDFRNTIIIMTSNVGAQELQDQRFAGFGASSDGQDY
                     ETIRKTMLKELKNSFRPEFLNRVDDIIVFHKLTKEELKEIVTMMVNKLTNRLSEQNIN
                     IIVTDKAKDKIAEEGYDPEYGARPLIRAIQKTIEDNLSELILDGNQIEGKKVTVDHDG
                     KEFKYDIAEQTSETKTPSQA"
     gene            complement(5516..6523)
                     /locus_tag="NBI57_001265"
     CDS             complement(5516..6523)
                     /locus_tag="NBI57_001265"
                     /EC_number="2.7.14.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein arginine kinase"
                     /protein_id="HCD1822221.1"
                     /translation="MTHNIHDNISQWMKSNEETPIVMSSRIRLARNLENHVHPLMYAT
                     ENDGFRVINEVQDALPNFELMRLDQMDQQSKMKMVAKHLISPELIKQPAAAVLVNDDE
                     SLSVMINEEDHIRIQAMGTDTTLQALYNQASSIDDELDRSLDISYDEQLGYLTTCPTN
                     IGTGMRASVMLHLPGLSIMKRMTRIAQTINRFGYTIRGIYGEGSQVYGHTYQVSNQLT
                     LGKSELEIIETLTEVVNQIIHDEKQIRQKLDTYNQLETQDRVFRSLGILQNCRMITME
                     EASYRLSEVKLGIDLNYIELQNFKFNELMVAIQSPFLLDEEDDKSVKEKRADILREHI
                     K"
     gene            complement(6513..7079)
                     /locus_tag="NBI57_001266"
     CDS             complement(6513..7079)
                     /locus_tag="NBI57_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000882074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UvrB/UvrC motif-containing protein"
                     /protein_id="HCD1822222.1"
                     /translation="MLCENCQLNEAELKVKVTSKNKTEEKMVCQTCAEGHHPWNQANE
                     QPEYQEHQDNFEEAFVVKQILQHLATKHGINFQEVAFKEEKRCPSCHMTLKDIAHVGK
                     FGCANCYATFKDDIIDIVRRVQGGQFEHVGKTPHSSHKKIALKRKIEEKNEYLKKLIE
                     IQDFEEAAIVRDEIKALKAESEVQHDDA"
     gene            complement(7098..7559)
                     /locus_tag="NBI57_001267"
     CDS             complement(7098..7559)
                     /locus_tag="NBI57_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000551761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HCD1822223.1"
                     /translation="MHNMSDIIEQYIKRLFEESNEDVVEIQRANIAQRFDCVPSQLNY
                     VIKTRFTNEHGYEIESKRGGGGYIRITKIENKDATGYINHLLQLIGPSISQQQAYYII
                     DGLLDKMLINEREAKMIQAVIDRETLSMDMVSRDIIRANILKRLLPVINYY"
     gene            7717..8931
                     /locus_tag="NBI57_001268"
     CDS             7717..8931
                     /locus_tag="NBI57_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HCD1822224.1"
                     /translation="MHILIGIIGIIFFLALAFLFSSDRKNIRWKYVGILLAIQLIFAF
                     ILLKTTMGIKVIGGISNGFNYLLLKAADGVNFVFGGIQYIDPKQPPFFFSVLLPIVFI
                     SAIIGILQYTKILPLIINVLGFLISKINGMGRLESYNAVAAAILGQSEVFISIKKQLP
                     YIPKQRLYTLTASAMSTVSASIIGAYFTLIEPKYVVTAVVLNLFGGFIIASIINPYKV
                     NEEDDKLLVEETEERQQSFFEMLGEYILDGFKVAVIVGAMLIGYIAIIALLNGIVSNI
                     FSTVSGGAISWDFQTLIGFVFAPFAFLVGVPWQDAVQAGSVMATKLLSNEFVAMQALG
                     KLSDLSEHAKGVTSVFLVSFANFSSIGIISGAIKSLNDKKGDTVARFGLKLLFGATLV
                     SFISAAIAGFFI"
     gene            complement(9139..9699)
                     /gene="pdxT"
                     /locus_tag="NBI57_001269"
     CDS             complement(9139..9699)
                     /gene="pdxT"
                     /locus_tag="NBI57_001269"
                     /EC_number="3.5.1.2"
                     /EC_number="4.3.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase glutaminase
                     subunit PdxT"
                     /protein_id="HCD1822225.1"
                     /translation="MKIGVLALQGAVREHIRHIELSGHEGIAVKKVEQLEEIEGLILP
                     GGESTTLRRLMNLYGFKEALQNSTLPMFGTCAGLIVLAQDIVGEEGYLNKLNITVQRN
                     SFGRQVDSFETELDIKGIATDIEGVFIRAPHIEKVGQGVDILCKVNEKIVAVQQGKYL
                     GVSFHPELTDDYRVTDYFINHIVKKA"
     gene            complement(9703..10590)
                     /gene="pdxS"
                     /locus_tag="NBI57_001270"
     CDS             complement(9703..10590)
                     /gene="pdxS"
                     /locus_tag="NBI57_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011303857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase lyase subunit
                     PdxS"
                     /protein_id="HCD1822226.1"
                     /translation="MSKIIGSDRVKRGMAEMQKGGVIMDVVNAEQARIAEEAGAVAVM
                     ALERVPSDIRAAGGVARMANPKIVEEVMNAVSIPVMAKARIGHITEARVLEAMGVDYI
                     DESEVLTPADEEYHLRKDQFTVPFVCGCRNLGEAARRIGEGAAMLRTKGEPGTGNIVE
                     AVRHMRQVNSEVSRLTVMNDDEIMTFAKDIGAPYEILKQIKDNGRLPVVNFAAGGVAT
                     PQDAALMMELGADGVFVGSGIFKSEDPEKFAKAIVQATTHYQDYELIGRLASELGTAM
                     KGLDINQLSLEERMQERGW"
     gene            10694..12076
                     /locus_tag="NBI57_001271"
     CDS             10694..12076
                     /locus_tag="NBI57_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HCD1822227.1"
                     /translation="MKNTLYHQLYEKLKKQIIEGQFKEGDKFYSKRQLSKHLSISQTT
                     VEHAYQLLLDEGYIYSRPRSGYFVSEIESLTILNNQPIPSLFDDDSYKPKASDEAYDY
                     AFNLDEIDTKHFPIELFRKYSKDLYDTNHLNQLRRGHFQGELHLRFQLAFYLFTNRGV
                     ICDPNQIIIGSSTEQLVNQLVDLLYTSTFIIEKPSYPPIKNILDKKQVEYEQIEVEDN
                     GINVDEVIKSQKNIVYITPSHQFPTGYVMDLKKRTQLIQWAQEKEERFIIEDDYDSEF
                     RYFGKPIPAIQGLYSRGEKVIYISTFSKSIFPSCRVAYMVLPYSIMKKYHSQNHIEGN
                     TVPVHMQNLIATFISSGGFERHLNKMRRIYRRKLTYILKRLKPYKEQLDIQGAETGMH
                     FTITVKNGLTLQECLDRANKVKLKLQVYNFDDDQDYKKTPKFILGFGGIDDDALKPHV
                     DALIKSLIIK"
BASE COUNT         3747 a         2283 c         1686 g         4410 t
ORIGIN      
        1 gatatgtttt gcatttattt atagttatac ttcaaattac aaacttcgcc atttcatata
       61 ccttttaata tctattttgt tttcgtcaac tacagttttt ataatgatac tgtatcttcg
      121 atttttttag caaaaacaat tcttcctgaa gatgtttgca ataagctgac tacttctaaa
      181 ttgacatgac tgccaataag atttttagca ttatcaacaa ctaccatcgt accatcatct
      241 agatatccta ctgcctgacc aggctcttta cccatttttg tcagtaaaat atgcagttga
      301 tcaccttgat gtacattagg tttgattgct tctgataaat cattaacatt taatgctttg
      361 ataccatgta catgacaaac tttatttagg ttgaaatctg tcgtaataat acttgcatga
      421 tattgtttag ctagctttaa tagcattgta tcaatatcac tatgtgtttt agttggatgt
      481 ataacctttg taggataatc taaatcatac aattcattta aaatatctaa gcctctttta
      541 cccttttcac gtttaacact gtcatttgaa tctgcaacaa tttgtaattc attaataaca
      601 ccttgcggaa ttaaaatatt gccatcgata aaaccgcaac gaatgacttc taaaatacga
      661 ccatcaataa ttgcgcttgt gtcgataatt tttggcgtag cacttttagt atgttgtgac
      721 atggaacgcg ctatattctc aggtaaaaac attaacattt catctcgttt tttaaggcca
      781 aattggaaac cgaaataaca tagtaatatc gtaattatga caggaatgaa atgattaaaa
      841 atagagttac caattgattc taatataaac gacaccataa cagaaataag taatccgatt
      901 attaaaccta ttgttgcgaa tagtatttca acagcacttc tacgcataat aaaatgttct
      961 aaacctttta tagcgttagt aactcgtcta ataaatacac caaaaattaa gaacataaaa
     1021 atactaccga taatgccatc tacatagtga ttttttaaaa agctggagtt ttgtaatcca
     1081 agatcatttg caatttcagg aataataatt attcctaatg cgctcccaat aattaagtaa
     1141 ataataataa ccattagttt aacgatattc acacaatgtc ctcctttctt gatgttttat
     1201 gaatgaagag caaatgacaa tgcttcatgt acagtagtta cacctattac ttgtatacct
     1261 tcaggatatg tccatccgcc tatattattt ttaggaataa ttacacgttt gaaacctagt
     1321 tttgcagcct cttgcacgcg ttgttctatc cgagatacac gacgtacctc acccgttaaa
     1381 ccaacttctc caatatagca atctaatccg tcgacagctt tatctttaaa gctagatgca
     1441 gttgctacaa ttacacttaa atcaactgct ggctccgtta actttacacc gccagctact
     1501 ttgatataag catcttgttg ttgtaataga taattttctt tcttttccaa aacagccatc
     1561 aacaaactta atcgattatg atcaatccct gttgccattc gtctcggatt gttaaaagtc
     1621 gttggagtta ccagcgcttg aacttctatt aaaagtggtc tggttccctc catggttgca
     1681 acaattgttg aacctggaac atttgttgaa cgttcttcta aaaacatttc agatggatta
     1741 tttacacctt ttaatccact ttgcttcatt tcgaagattc ccatttcatt cgttgaacca
     1801 aaacggtttt taacagctcg caaaattcga tatgcgtggt gttcatcgcc ttcaaaataa
     1861 agcacagtat caaccatgtg ttctagcaat cttgggccag caatttgacc ttctttcgtt
     1921 acatgaccca ctataaaagt tgcaatgttc atttgtttag caatattcat taaactttgt
     1981 gtactttcac gaacttgtga aacagaacct ggcgcagagc tgatttcagg atgatatatt
     2041 gtttgaatcg aatccactac taataaatca ggttgttctt cttttactgt ttgataaata
     2101 acttcaagat ctgtttcagc taatacttgc aattcacttg aatcttcatc taatcgctct
     2161 gcacgtaatt tagtctgact aagcgattct tctccagtaa tatatagtac ttttttcttt
     2221 tgagataacg atgcacaaat ttgtaaaagt aacgttgact taccaatacc tggatcccca
     2281 ccaataagta ctaacgatcc gctcacaata cctccaccta atacacggtt gaattctgct
     2341 gaatctgtta acactctcgg cgttgtttca tgtttaatac tatttaattt ttgtacttta
     2401 cctgctaatt ccttggtttt aactccatgt ttaggattgg ctgctttttc aacaatttcc
     2461 tccatttgat tccaagcgcc acaattagga catttcccca tccatttagg agattgataa
     2521 ccacaagcca tacattcaaa aatcactttt ttcttggcca aaattgcacc tccactttcc
     2581 tttgaacaaa tctattttaa acgcacattc ccattatgac aaattaaatg tgagttacat
     2641 ttgtttgtat tttaacatga ctactaacgc aaactgtttt accagattta ttttttatga
     2701 atattataaa ataaacttac atcatgttga tagtcggtat ctttcaaaga taaaatctta
     2761 ataatttctt agtaaactct tttctctaga tttatcacaa tattatatag acctatttta
     2821 ttttgacgta agttgctagt atcttcaaac aaaaaacctt tataaaaatt cataccttta
     2881 tgctatcgct gtaggctcat taacttgtta catataattc ttaactatcc tttgatgatt
     2941 gttttattag attgtttcgt tgatggatac tttcacgaat ttctatagtt caatgctact
     3001 aaaaaaacag cccaaaactt taatttgttt tggactgttt tataattatg cttgcgatgg
     3061 tgttttagtt tctgaagttt gttcagcaat gtcatattta aactctttac catcatgatc
     3121 tactgtaact ttcttacctt caatttgatt accatctaat attaattcac ttaaattatc
     3181 ttcgatagtt ttttgtatcg ctctaattaa tggtcttgca ccatattctg gatcatatcc
     3241 ttcttctgcg attttgtctt tcgctttatc agtaacaata atatttatgt tttgttcaga
     3301 taatcgattt gttaatttat taaccatcat tgttacaatt tcttttaatt cttcttttgt
     3361 tagtttatgg aatacaatga tatcatctac acggtttaaa aattctggac ggaatgaatt
     3421 ttttaattct tttaacatcg tttttcgaat tgtttcataa tcttgtccat cacttgaagc
     3481 accgaatcca gcaaatcgtt gatcttgtaa ttcttgtgcc ccaacgtttg atgtcattat
     3541 gataattgta tttctgaaat caactgtacg tccttttgta tctgtcaaat gtccatcatc
     3601 taaaacttgt aatagaatat taaatacatc tggatgagct ttttcaattt catcaaataa
     3661 aattacagaa tatggtttac gtctaacttt ttcagttaat tgtccaccat catcatgacc
     3721 aacatatcct ggaggagcac caactaatcg gctcactgcg tgtttttcca taaattcact
     3781 catgtctaca cggatcatcg catcatcatc gccaaacatt gattcagcta aagctctagc
     3841 taattcagtt ttaccaacac cagttggtcc aaggaagata aagctaccaa ttggtcgttt
     3901 aggatctttt aaccctgcac gggcacgtct aaccgcttta ctgattgaat taacagcatc
     3961 tttttgccca ataactctct catgtaatgt atcttctaga ctaagaagtt tttcagattc
     4021 tgtttcattg attttagtta atgggatacc tgtccatcct gcaataactt cagcaatatc
     4081 ttcttctgac aatgaagttg acatgccatt ttgtgcattc ttccattcat ttttagcttc
     4141 ttcatattgc ttttcaagtt ttgtttgttt atcacgcagg ttagcagcat tttcaaactc
     4201 ttgagcatgt actgcggcat ctttttcatt tttaactttt tcaatttctt gttcaatttc
     4261 ttttaaatta ttaggtgtcg tatgactctt aagtcttact ttagaacttg cttcatcaat
     4321 taaatcaatt gctttatctg gtaagaaacg atctgaaacg tatctgttac ttaatttaac
     4381 agctgcttca atagcttcgt ctgaaatatt aatacgatgg tgtgcttcgt aacgatctct
     4441 taatcctttt aaaatagcaa ctgtatctac tactgaaggt tcatcaactt gtacaggttg
     4501 gaaacgacgt tctaaagccg cgtctttttc aatatttttg cgatattcat ctaatgtagt
     4561 agcaccaata cattgtaatt caccacgtgc taatgccggt ttcaaaatat tcgaagcatc
     4621 gatagcacct tcagcaccac cagcaccaac taaagtatgc aactcatcaa taaataggat
     4681 gacattacct gcttgttgga tttcttccat aacctttttc agacgctctt caaattcacc
     4741 acgatattta gtacctgcaa ctactgttcc catatctaaa gacataacac gcttatcttt
     4801 taatgtctct ggtacctcat tattcactat ggcttgcgct aaaccttcag caatagcagt
     4861 tttaccaaca cctggctctc caataagtac aggattgttt ttcgtacgtc tacttaatac
     4921 ttcaattaca cgtgtaattt ctttatcacg tcctataaca ggatctaatg taccgtcttt
     4981 ggcaatgact gttaagtcac gagctaaact atctaaagtt ggagtattat ttgacttact
     5041 agcttgtgca tttttattac tcatttcagg gtttcctaaa gctttcacaa cttgtgcacg
     5101 tgctttagta atatttaaat ctagatttgc aaaaactctt gctgcaacac cttcattttc
     5161 acgaatcaag cctaataaaa tatgttccgt tccaacaaaa ttgtgatgta attttctagc
     5221 ttcatccatc gataattcaa tgactttttt agctctaggt gtataatgca atgtaccaac
     5281 atgatcttga ccatgtccga ttaatttttc aacttcttca attactttat cttcagtgat
     5341 attaaaactt tctaatactt ttgcagcaat tccttcaggt tctttcatta accccaataa
     5401 taggtgttct gttcctatat ttgaatgatt taaacgaatt gcttcttctt gtgcatgtgc
     5461 taatacgcgc tgtgcacgtt cagttaatct accaaataac ataaataatg acctcctact
     5521 ttatatgttc tcttagtata tctgctcgtt tttcttttac agatttgtca tcttcttcat
     5581 ctaataaaaa tggtgactgt atagctacca tcaattcatt aaatttaaag ttttgtaatt
     5641 caatgtaatt taaatctata ccaagtttaa cttcgcttaa tctataagaa gcctcttcca
     5701 tagttatcat tctacagttt tgtaaaatac ctagcgagcg aaaaacacgg tcttgtgttt
     5761 ctaattgatt ataagtgtct aacttttgtc gtatttgttt ttcatcatga atgatttgat
     5821 taacaacttc tgttaatgtt tctatgattt ctaactcaga tttaccaagt gtaagttggt
     5881 tggatacttg ataagtatgt ccataaactt gcgaaccttc accgtaaata cctctgattg
     5941 tatatccaaa acgattaatg gtttgagcaa tccgtgtcat tcttttcata atagatagac
     6001 ctggtaaatg tagcatcacg cttgctctca taccagtacc tatattggta ggacatgtag
     6061 ttaaataacc aagttgttca tcataactta tatcaaggct tcgatctaat tcatcatcaa
     6121 ttgatgaagc ttgattatat aaagcctgta atgtcgtgtc agttcccata gcttgaatac
     6181 gaatatggtc ctcttcattt atcatgacac ttaaagattc atcatcattc actaatactg
     6241 cggctgctgg ttgttttatt agttcaggac taatcaaatg ctttgcaacc attttcattt
     6301 tactttgttg atccatttga tcaagacgca ttaattcaaa atttggcaag gcatcttgta
     6361 cctcatttat aactctaaat ccatcatttt cagtagcata cattagtgga tgcacatgat
     6421 tttctaaatt acgcgctaac cgaattctag aagacataac aattggtgtt tcttcattac
     6481 ttttcatcca ttggctgata ttatcatgaa tattatgcgt catcatgttg cacctcactc
     6541 tcagctttta gtgctttaat ttcatctcta acaatggctg cttcctcaaa atcttggatt
     6601 tcaataagtt ttttcaaata ttcattcttt tcttcgattt ttcgctttaa agctatcttt
     6661 ttatgtgaag aatgtggtgt ctttccaacg tgctcaaatt gtccaccttg aactctgcgg
     6721 acgatatcaa tgatgtcatc tttaaatgtt gcataacaat tagcacaccc aaatttacca
     6781 acatgtgcaa tatctttcaa agtcatatga catgatgggc aacgtttttc ttctttaaac
     6841 gctacttctt gaaaattaat gccatgtttc gtagctaaat gttgtaaaat ttgcttaaca
     6901 acaaatgctt cttcgaaatt atcttgatgt tcttgatatt caggctgttc attagcttga
     6961 ttccacggat ggtgcccctc agcacaagtt tgacacacca ttttttcttc tgttttattt
     7021 ttacttgtaa ctttaacttt taattccgct tcattaagtt gacaattttc acaaagcact
     7081 tcaacacctc atttcattta gtaataattt ataactggta acaaacgttt taaaatattt
     7141 gctctaataa tatctctaga aaccatatcc attgatagcg tttctctatc aataactgct
     7201 tgaatcattt tagcttcacg ttcatttatt aacattttat ctaaaagccc atcaataata
     7261 taataagctt gttgttgaga aatagaaggt ccaatcagct gaagcaaatg attaatataa
     7321 cctgttgcat ctttattttc aattttagtg attcggatgt aaccaccacc accacgttta
     7381 ctttcgattt cataaccatg ttcattagtg aatcgtgttt tgattacata atttaattgt
     7441 gatggtacac aatcaaaacg ctgtgcgata ttcgctctct gaatttcaac gacatcttca
     7501 ttcgactctt caaataaacg tttgatgtat tgttctatga tgtcagacat attgtgcatg
     7561 tatatcaccc ctttttgacc ttctttgact atattatatg acctattttg acctttttca
     7621 attaatttgt ttttatattt tcaaaaattt atgatgttag cccttccatt ttattgtatg
     7681 atagattgta gaaaaaacta aaggaatgag aaatatatgc atattctaat tgggattatc
     7741 ggaattatct tctttttagc acttgcgttt ctatttagct cagacaggaa aaatattcgc
     7801 tggaaatatg taggaatttt acttgcaatt cagcttatct ttgcatttat cttgcttaaa
     7861 actacaatgg gaatcaaggt tattggtggt atttcaaacg gttttaacta cttattatta
     7921 aaagcagcag atggtgttaa ctttgtattc ggtggtattc aatatatcga tcctaagcaa
     7981 ccaccattct tcttcagtgt attattacct atcgtattta tttcagcgat tatcggtatc
     8041 cttcaataca caaagatttt acctttaatc attaatgttt taggattctt aatttctaaa
     8101 attaatggta tgggacgttt agaatcatac aatgcagttg ctgcagcaat tttaggacaa
     8161 tctgaagtat ttatttcaat taaaaaacaa ttaccataca tacctaagca acgtttatac
     8221 acattaacag cttcagcaat gtcgactgtt tctgcttcaa ttatcggtgc atactttacc
     8281 ttaattgaac ctaaatatgt tgtaacggct gttgtcttaa acttatttgg tggtttcatc
     8341 atcgcttcaa ttattaaccc ttataaagtt aatgaagaag atgacaaatt attagttgaa
     8401 gaaactgaag aaagacaaca atcattcttc gaaatgctag gagaatatat tttagatgga
     8461 tttaaagtag ctgtcattgt aggtgcaatg ttaatcggat acattgccat tatcgctcta
     8521 ttaaatggaa ttgtaagcaa tatctttagt acagtttcag gtggcgctat ttcttgggat
     8581 ttccaaacat taattggttt tgtctttgca ccatttgcat tcttagtcgg tgtgccatgg
     8641 caagatgcag ttcaagccgg ttcagttatg gcgacaaaac tattatctaa cgaatttgta
     8701 gcaatgcaag cacttggtaa acttagtgat ttatctgaac acgcaaaagg tgttacatct
     8761 gtattcttag tatcatttgc taactttagc tctattggta ttatttcagg tgctattaaa
     8821 tcacttaatg acaaaaaagg agatacagtt gcccgctttg gtttgaaatt attattcggt
     8881 gcaacactag tatctttcat ttcagctgca atcgctggat tctttattta acaaatacat
     8941 caacatcgaa ttaatccctt caaaattttg actattctca gtctctttga aggggttttt
     9001 tcttatgtat atctttattt tttatcattt ttaattttga cataaaaaat aactcctcca
     9061 aagctaagtt tgaaggagat attttcttct caacaaatct ataaatatca ataattcatt
     9121 gatttagcat acattaagct atgctttttt tacaatatga ttaataaagt aatcagttac
     9181 tctatagtca tctgttaatt caggatggaa tgatacgcct aaatatttac cttgctggac
     9241 agctacaatt ttctcattaa ccttacatag gatatctacg ccttgaccta ctttttcaat
     9301 atgtggcgct cttataaaga caccttcaat atctgtagcg atacctttaa tatctaattc
     9361 tgtttcaaag ctgtcaactt gtctaccgaa tgagtttcgt tgtacagtaa tattcaactt
     9421 gttaaggtat ccttcttcac caactatatc ttgcgctaga actattaatc ctgcgcatgt
     9481 accaaacata ggtaaagttg aattttgtaa agcctcttta aatccatata aattcattaa
     9541 tcgacgtaac gttgtagact cgccaccagg taatattaag ccctcgattt cttctaattg
     9601 ttcaactttt ttaactgcaa taccttcatg accacttaat tcaatatgtc taatatgttc
     9661 acgtactgca ccttgtaatg ctaatacacc tattttcata tcttaccaac cacgctcttg
     9721 catacgttct tctaatgata attgattgat atctaaacct ttcatagcag tgccaagttc
     9781 acttgctaat cttccaatta gttcatagtc ttggtaatgt gttgttgctt gaacaattgc
     9841 tttagcaaat ttttctggat cttctgattt aaaaatacct gatccaacga atacaccgtc
     9901 agcacctaat tccatcatta aagcagcatc ttgaggagtc gcaacgccac cagctgcaaa
     9961 gttaactacc ggtaaacgac cattgtcttt aatttgtttt aaaatttcat aaggcgcacc
    10021 gatatctttc gcaaaagtca taatctcatc atcattcatt acagtcaatc gactaacttc
    10081 tgaattaact tgtctcatat gtcttacagc ttcaacaata ttacctgtac ctggttcacc
    10141 tttagtacgt aacatagcag caccttcacc aattctacgc gcagcttcac ctaaattacg
    10201 acatccacat acaaatggta ctgtaaattg atcttttctt aagtgatatt cctcatctgc
    10261 tggtgttaac acttctgatt catcaatata gtcaacaccc atcgcctcta atactcttgc
    10321 ttcagtgata tgaccaatac gtgctttagc catgactgga atagaaacag cattcattac
    10381 ttcttctaca attttagggt ttgccatacg tgcaacgcca ccagcagctc taatatcaga
    10441 aggtactcgt tctaatgcca taactgctac cgcgcctgct tcttctgcaa ttcttgcttg
    10501 ctcagcatta acgacatcca taataacgcc gcctttttgc atttcagcca tacctctttt
    10561 gactctgtct gatccaataa ttttactcat tatacataaa accccttttc aagttgatta
    10621 aatacattgt aatgtataaa ctgatattta aaaaggtaca aaataagata aatttaggag
    10681 gtcagttata aaattgaaaa acactttata ccatcaactt tatgaaaaat taaaaaaaca
    10741 aataattgaa ggtcaattta aagaaggcga taaattttat tctaaaagac aattaagcaa
    10801 acatttatct atcagccaaa caaccgtaga gcatgcatat caacttctat tagacgaagg
    10861 ttacatttat tcaagacctc gctctggata ttttgtgtcc gaaattgaat ctttaacaat
    10921 tttaaacaat caacctatcc cctctctttt tgatgatgat tcatataaac caaaagcgag
    10981 tgatgaagct tatgattatg cttttaattt ggatgaaata gatacgaaac atttccctat
    11041 agaacttttc agaaagtatt ccaaagatct ttatgataca aatcatttaa atcaattacg
    11101 tagaggacat tttcaaggag aattacattt aagatttcaa ctggcatttt atttatttac
    11161 taatcgtggt gtaatttgtg atcctaatca aattataatt ggctcatcta ccgaacaatt
    11221 agtaaatcaa ttagtcgatt tactttatac ttcaacgttt attatcgaaa aacctagtta
    11281 tccaccaatc aaaaatattt tagataaaaa acaagttgaa tatgaacaaa ttgaagttga
    11341 agataatgga ataaacgtag atgaagttat taaatcacaa aaaaatatag tatatataac
    11401 gccttcccat caatttccaa ctggatatgt gatggactta aaaaaacgaa ctcaattaat
    11461 acaatgggct caagaaaaag aagaacgatt tattatagaa gatgattatg attccgagtt
    11521 tagatatttc ggcaaaccca ttccagcaat tcaaggtcta tattcaagag gagaaaaagt
    11581 gatttatatc agtacatttt caaagtctat ttttcctagc tgtcgagttg catatatggt
    11641 gttaccctat tctataatga aaaaatatca ttctcaaaat cacattgaag gcaacactgt
    11701 acctgttcat atgcaaaacc ttattgcaac gtttatatct tccggtggtt ttgaaaggca
    11761 tttaaataaa atgagacgaa tttatcgtag aaaactaacc tatattttaa aaagattgaa
    11821 accttacaaa gagcagcttg atattcaagg tgctgaaacg ggtatgcatt ttacaattac
    11881 agtaaaaaat ggattaacat tacaagagtg tcttgataga gcaaacaaag taaagttaaa
    11941 attacaagtt tataattttg atgatgatca agactataag aaaaccccta aatttatatt
    12001 aggctttggc ggtatagacg atgatgcttt aaaaccgcat gtagacgctc taataaaatc
    12061 attaattata aagtaacact tctttgataa aagttagtgt tacttatgca atagcttgtc
    12121 tattgt
//
LOCUS       DAHHSC010000060        11877 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.060, whole genome shotgun sequence.
ACCESSION   DAHHSC010000060 DAHHSC010000000
VERSION     DAHHSC010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 11877)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11877)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11877
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.060"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            2..130
                     /locus_tag="NBI57_001272"
     CDS             2..130
                     /locus_tag="NBI57_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1822228.1"
                     /translation="MIKTMSIENMRTYQQRVNNLWIKGVDNVIYCGYQVGKQKINN"
     gene            complement(275..2203)
                     /gene="abc-f"
                     /locus_tag="NBI57_001273"
     CDS             complement(275..2203)
                     /gene="abc-f"
                     /locus_tag="NBI57_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein"
                     /protein_id="HCD1822229.1"
                     /translation="MILLQLNHISKSFDGEDIFTDVDFEVKTGERIGIVGRNGAGKST
                     LMKIIAGVENYDSGNVSKIKNLKLGYLTQQMTLNSNATVFEEMSKPFEHIKRMESLIK
                     EETDWLSKHANDYDSDTYKTHMSRYESLSNQFEQLEGYQYESKIKTVLHGLNFSEEDF
                     NKPINDFSGGQKTRLSLAQMLLNEPDLLLLDEPTNHLDLETTKWLEDYLRYFKGAIVI
                     ISHDRYFLDKIVTQIYDVALGDVKRYVGNYEEFIQQRDLYYQKRMQEYESQQAEIKRL
                     ETFVEKNITRASTSGMAKSRRKILEKMERIDKPMLDAKSANIQFGFDRNTGNDVMHVK
                     NLEIGYQTAITKPISIEVSKGDHIAIIGPNGIGKSTLIKTIANQQKALNGDITFGANL
                     QIGYYDQKQAEFKSSKTILDYVWDQYPLMNEKDIRAVLGRFLFVQDDVKKIINDLSGG
                     EKARLQLALLMLQRDNVLILDEPTNHLDIDSKEMLEQALQHFEGTILFVSHDRYFINQ
                     LANKVFDLTIDGGKMYLGDYQYYIEKVEEAEALKAHQEEQSVNVQAHKKSMEQSSYHN
                     QKEQRREQRKLERQISECENEIETLETTILQIDEQLTQPEVYNNPQKANELAIQKQDS
                     EQKLEHAMSKWEELQQKL"
     gene            2456..3091
                     /locus_tag="NBI57_001274"
     CDS             2456..3091
                     /locus_tag="NBI57_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HCD1822230.1"
                     /translation="MSDQVKIPRATLKRLPLYYRFVSSLKSKGIDRVNSKAISDALQI
                     DSATIRRDFSYFGELGKKGYGYNIDSLLDFFKSELSESDMIKIAIVGVGNLGKALLTY
                     NFSIHDDMTITEAFDVKEDVIGQKIGNVIVKDNDELITTLKKEEIDVVILTTPERVAQ
                     KVADELVQAGVKGILNFTPGRINTPSDVQVHQIDLGIELQSLLFFMKNYSE"
     gene            3395..4474
                     /locus_tag="NBI57_001275"
     CDS             3395..4474
                     /locus_tag="NBI57_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000266877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE family protein"
                     /protein_id="HCD1822231.1"
                     /translation="MWMIFSGLIVGGLLGFVMQRTRFCLTGGFRDMYVQKNNKMFYAL
                     LIAITIQSIGLLILTATDILQIPAHSFPILGTIIGSFIFGIGIVLAGGCATGAWYRAG
                     EGLIGSWIALVLYAVTAAITKTGILKPVMDKINQPTNVNSDMSQTTGIPFWGLVVILT
                     IITIFLVVRTLNNKKVRVAVPKLKQRYTGIRYYLFEKRYHPFIAAIVIGLIALLAWPM
                     SASTGRNDGLGITTPSANLVHFLITGETKFIDWGVFLVLGIFIGSYIAARGSREFKWR
                     LPDKITIRNSAIGGICMGFGASVAGGCSIGNGLVETATMTWQGWIALASMIVGVWTMS
                     HFIFVRPMKKVQQQSAKVKQQTQIV"
     gene            4534..4758
                     /locus_tag="NBI57_001276"
     CDS             4534..4758
                     /locus_tag="NBI57_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HCD1822232.1"
                     /translation="MIHELGTVGMVCPFPLIEAQKKMATLQSGDELKIDFDCTQATEA
                     IPNWAAENGYPITNYEQIDNASWTITIQKV"
     gene            4966..6216
                     /locus_tag="NBI57_001277"
     CDS             4966..6216
                     /locus_tag="NBI57_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HCD1822233.1"
                     /translation="MNLNDTIFMFLCTLLVWLMTPGLSLFYGGLVQSKNALNTVMQSM
                     AAIVLVTFVWITVGFTISFGNGNLWFGNWEYTFLNHVGFATQEDISPHIPFALFMLFQ
                     MMFCTIAISILSGSIAEKMKFIPYLLFVVIWTALVYSPVAHWVWGGGWINKLGVLDFA
                     GGTVVHITSGVSGLVLAIMIGKGNKHSESTPHNLIITLIGGIFVWIGWYGFNVGSAFT
                     FDNIAMLAFTNTVISASAGAIGWLILEYIFKKTTSLLGLLLGALAGLVVITPAAGYVT
                     YLSATIMALIGGICCYIVINYIKVKLKYHDALDAFGIHGVGGIIGAVLTAVFQSKKAN
                     PDIENGFIYTGDIHIILVQILCVTAVVIFSIVMTFIIAKVIKLITPLSVTEQETNIGL
                     DKIVHGEHAYFEGELNRFNKHIRY"
     gene            6399..7349
                     /locus_tag="NBI57_001278"
     CDS             6399..7349
                     /locus_tag="NBI57_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCD1822234.1"
                     /translation="MKNISDIAKLAGVSKSTVSRFLNNGSVSKKTSEKLTRIIAEHDY
                     QPNQFAQSLRARQTHLIGAIIPRMNSYAVDETIKGLAKQCQKYESQLILNYTGLNIEA
                     EIQALETLARSKVDGIVLMATDITERHIEVINKMNVPIVIVGQQHEQLHSIVHDDYKA
                     GQIIGEWIGQQGYQQVEVFSVSEKDIAVGIHRKRGLLDQLAKYQIKPNIHETNFTYVE
                     AQKDVANVLENVEQVDAVVGATDTIALAAYKYYSDKNDVMKPHQIYGFGGDPMTQLVS
                     PSIKTIHYNYFEAGQCAMEEIQQMLKKQDMPYSVTVDVNI"
     gene            7498..8982
                     /locus_tag="NBI57_001279"
     CDS             7498..8982
                     /locus_tag="NBI57_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HCD1822235.1"
                     /translation="MTEWTREERYQRIEDVDTEYFKTLKQQVDQSKFRQQFHIQPETG
                     LLNDPNGLIFYKGKYYVSHQWFPLGAVHGLKYWYNYTSDDLINFKPEGPILNPDTKYD
                     SHGVYSGSAFEYNGHLYYMYTGNHRDNHWQRHASQMIARLKEDGSVEKFPKPVISQQP
                     EGYTSHFRDPKVFKYGEKYYAIIGAQNNDQQGRLLLYNTEDIINWHYLGEINTELDDF
                     GYMWECPDYFNLDNQDVILICPQGIEPKGNQFKNIYQSGYILGKFDIEKLTYEHENFV
                     ELDNGFDFYAPQTFLDEKGRRVLIGWMGLPEIEYPTDNEGWAHCLTIPRVLNVENGQL
                     KQRPYPALEKLRHNKETALGYANKFTRKLHPYEGKQYELIIDILDNDATEVYFELRTS
                     KTSSTLIAYNKRENKITLDRSDSGLLPTNVEGTTRSTILDTPLKQLQIFVDTSSIEIF
                     CNDGERVLTSRIFPTEDALGIKTSTESGQVYLQFTKYDLKDEHK"
     gene            8979..9938
                     /locus_tag="NBI57_001280"
     CDS             8979..9938
                     /locus_tag="NBI57_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase"
                     /protein_id="HCD1822236.1"
                     /translation="MRRLFSIGEALIDFIPNVTNANLKDVQTFTKQIGGAPCNVACTV
                     QKLGQQAYMITQLGNDAFGDSIIETISSIGVDVSKVYRTNEANTALAFVSLTEAGERD
                     FSFYRKPSADMLFEPSFVNDIDVNENDVVHFCSVDLVDSPMRDAHYQLITKTLNANGT
                     VVFDPNVRLPLWDNAEDLRQTIHTFLPLAHIVKVSDEELEFITGIHDENEAIQSLFTG
                     NVTVVIYTKGADGAAVYLKNGINHYHSGYKVKPVDTTGAGDAFIGAVISRILATDVLN
                     LTQLFENEGEEILAFSNRVAAIVTTKYGAINSLPTLQEVEQAF"
     gene            complement(10126..10235)
                     /locus_tag="NBI57_001281"
                     /pseudo
     CDS             complement(10126..10235)
                     /locus_tag="NBI57_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500747.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(10307..11023)
                     /locus_tag="NBI57_001282"
     CDS             complement(10307..11023)
                     /locus_tag="NBI57_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000688500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCD1822237.1"
                     /translation="MKIFICEDDPKQRENMVTIIKNYIMIEEKPMEIALATDNPYEVL
                     EQAKNMNDIGCYFLDIQLSTDINGIKLGSEIRKHDPVGNIIFVTSHSELTYLTFVYKV
                     AAMDFIFKDDPAELRTRIIDCLETAHTRLQLLSKDNSVETIELKRGSNSVYVQYDDIM
                     FFESSTKSHRLIAHLDNRQIEFYGNLKELSQLDDRFFRCHNSFVVNRHNIESIDSKER
                     IVYFKNKEHCYASVRNVKKI"
     gene            complement(11042..>11877)
                     /locus_tag="NBI57_001283"
     CDS             complement(11042..>11877)
                     /locus_tag="NBI57_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HCD1822238.1"
                     /translation="ISFVLFATFAFFYIYSTNTSSNGDSLIPYALVFIGLIIFISVVI
                     LIMSLFTLKEMKYKRNQEEIETYYEYTLKIEAINNEMRKFRHDYVNILTTLSEYIRED
                     DMIGLRAYFNKNIVPMKDNLQMNAIKLNGIENLKVREIKGLITAKILRAQEMNIPISI
                     EIPDEVSSINLNMIDLSRSIGIILDNAIEASTEIDDPIIRVAFIESENSVTFIVMNKC
                     ADDIPRIHELFQESFSTKGEGRGLGLSTLKEIADNADNVLLDTIIENGFFIQKVEIIN
                     N"
BASE COUNT         4000 a         1691 c         2117 g         4069 t
ORIGIN      
        1 tttgataaaa acaatgtcaa tagagaacat gcgtacttat caacaaagag tgaataactt
       61 gtggataaaa ggtgttgata acgtcattta ttgtggatat caggtgggaa aacagaaaat
      121 aaataattga ttagtgttaa tttacttgtg gataactagg ataagtctgt ggatagctat
      181 aatatcagcg tttttattgt gaataaaaaa aattaaaatt tgtaaaaaaa taactctaac
      241 tatagcgaat ttacagttag agttatgtat atttttatag tttttgttgt aattcttccc
      301 atttagacat agcgtgttct aatttttgtt cgctatcttg tttttggata gctaattcgt
      361 ttgctttttg tggattgtta tacacttctg gttgggttaa ttgctcgtct atttgtaaga
      421 tagtagtctc taaagtttct atttcatttt cacactcgga tatttgtcgt tcaagtttgc
      481 gttgttcacg tctttgttct ttttggttat gatacgacga ttgttccata gactttttat
      541 gtgcttgtac gttaactgac tgttcttctt ggtgcgcttt caacgcttcg gcttcctcaa
      601 ctttttcaat gtaatattga taatctccta aatacatctt tccgccatca attgttaaat
      661 caaatacctt gtttgctaat tggttaataa agtaacgatc atgggaaaca aatagtattg
      721 ttccttcaaa atgttggagt gcttgctcta acatttcttt tgaatctata tcgaggtggt
      781 tggtaggttc atctaaaata agtacattgt cacgttgcaa cataagtagt gctagttgta
      841 aacgtgcttt ttcaccacca gataaatcat taattatctt tttaacatcg tcttgtacaa
      901 ataagaaacg tccaagaact gctcgaatat ctttttcatt cattaacgga tattgatccc
      961 acacataatc taaaatcgtt ttactagatt taaattctgc ttgcttttga tcataataac
     1021 caatttgtaa atttgcgccg aaagtaatat cgccattaag cgctttttgt tgattagcaa
     1081 tagttttaat taaggtcgat tttccaatac catttggccc aatgattgct atatgatcgc
     1141 ctttagagac ctctatactg ataggtttgg taattgcagt ttgataaccg atttctaaat
     1201 tttttacatg catgacgtca ttacctgtat tccggtcaaa gccaaattga atatttgcac
     1261 ttttggcatc taacattggt ttatcaatgc gttccatttt ttctaaaatc ttacgtctac
     1321 tttttgccat tccacttgtt gaagcacggg taatattttt ctcaacaaaa gtttctaatc
     1381 gttttatttc tgcttgttga ctttcatatt cttgcattcg tttttgataa tataaatccc
     1441 gttgctgtat aaattcctcg taattaccaa catagcgttt gacatcacct aaagccacat
     1501 cataaatttg agtaactatt ttatctaaaa agtaacgatc atggctgatg atgacgattg
     1561 cacctttaaa ataacgtaga taatcttcaa gccactttgt cgtttctaaa tctaagtggt
     1621 tagtaggttc atctaaaagt aataaatcag gttcgtttaa tagcatttga gctaaagata
     1681 aacgtgtttt ttggccgccg ctaaaatcat tgataggttt attgaaatct tcttcactaa
     1741 aatttaaccc gtgaagtact gttttaattt tactttcata ttgatatcct tctaattgtt
     1801 caaattgatt cgataaagat tcataacggg acatatgtgt tttatatgta tcactatcgt
     1861 aatcatttgc atgttttgat aaccaatctg tttcttcttt gattaagctt tccattcgtt
     1921 taatatgttc aaatggtttt gacatttctt caaaaactgt tgcattagaa tttaaagtca
     1981 tttgttgagt taaataacct agttttaagt ttttaatttt tgacacattt ccagaatcat
     2041 agttttctac accagctata attttcatca atgttgattt accggcgcca tttcttccta
     2101 cgataccaat tcgttcacca gtttttactt caaaatcaac atcagtaaat atatcttcac
     2161 catcgaacga ttttgatata tgattgagtt gtaaaagtat cattcgatct tcacctttcg
     2221 tctttctcaa attttattaa cttatgaaaa aagtcgtaat tattacatta ataaccattt
     2281 tacagtataa aacgccgtct tgaaactaag atatttattt ttaaaattca ataatgtata
     2341 atatttaggt attcattgta atcaattatt tgttttttaa taatctaaca attacgctct
     2401 aactcgtttg aatacataga tataattcat tcaaacacga aggaggaaat agcgaatgag
     2461 tgaccaagtt aaaattcctc gagcaacttt aaaacgtttg ccgttatatt atagatttgt
     2521 cagttcatta aaatctaaag gtatagatcg tgtaaattca aaagcgatta gcgatgcgtt
     2581 acaaattgac tcggcaacaa ttcgtcgtga cttttcatat tttggcgaat taggtaaaaa
     2641 agggtacgga tataatatag atagtttatt ggatttcttt aaatctgaac taagcgagag
     2701 tgacatgatc aaaatcgcaa ttgtcggagt tgggaaccta gggaaagctt tgctcacata
     2761 taacttttca atacatgacg atatgacgat tacagaagcg tttgacgtaa aagaagatgt
     2821 tattggccag aaaataggga acgttattgt aaaagataac gatgaattaa taacaacatt
     2881 gaagaaggaa gaaatagatg ttgtgattct aactacacca gaaagagttg cacagaaagt
     2941 tgcagatgaa ctcgtccaag ctggtgtgaa aggtatttta aacttcactc ctggtagaat
     3001 taatacgcct tcagatgtgc aagtacatca aattgactta ggtatagaat tacagtcatt
     3061 attattcttt atgaaaaatt acagtgaata aatgtgatgt tagctttgaa tgattaacct
     3121 tgttatatct ttaattaatt atagaacagt ctgggactaa gtatgtctca ggctttttct
     3181 gttatgtttg aaaatgaaag gaaaaactgt tgtgttgata aatatagtat aggttaataa
     3241 attaacacgt agagatataa tgatgaatcg taattaatcg ccttccttat atgaatataa
     3301 tataaatatc aaaaatattg tgtataaggt tgaaaacaag aaaatcatca cctataatac
     3361 atactaaact tatcggaaat gaggggttag gattatgtgg atgattttta gtggcttaat
     3421 tgtcggggga ctcttaggtt ttgtcatgca aagaacaaga ttttgtttaa caggtggctt
     3481 tcgagatatg tatgtgcaaa agaataataa gatgttctat gcattattaa tcgctattac
     3541 tattcaaagt ataggattat tgattttgac ggcaacagat attttacaaa ttcctgcaca
     3601 tagttttcca atattgggaa caattatagg ttcttttatt tttggaattg gaatagtatt
     3661 ggctggaggg tgtgcaacag gtgcttggta tcgcgctggt gaagggctaa ttggtagttg
     3721 gattgcatta gtattatatg ctgttactgc agcaatcact aaaacaggga ttttaaagcc
     3781 agtaatggat aaaattaatc aaccaacgaa tgtaaatagt gatatgtctc aaacaactgg
     3841 cattccgttt tggggattag tcgttatatt aactataatc accatttttc tagttgtaag
     3901 aacactcaat aataagaaag ttcgagttgc tgttccaaaa ctaaagcaac gttatacagg
     3961 tattagatat taccttttcg aaaaacgata ccatccattt attgcagcaa ttgtaattgg
     4021 acttatcgca ctcttagctt ggccaatgag tgcatcaact ggaagaaatg acggtttagg
     4081 tataacaacg ccttcagcaa atttagtaca ctttttgatt acaggtgaaa ctaaatttat
     4141 tgattggggt gtctttttag ttctaggaat tttcattggt tcatatattg cagctagagg
     4201 atcaagagaa tttaaatggc gattgccaga caagattaca atacgaaaca gtgccattgg
     4261 tggcatatgt atgggatttg gtgcgtcagt tgctggtggt tgttctatcg gtaacggttt
     4321 ggttgaaacg gcaacgatga cttggcaagg atggattgcg ctagcatcga tgatagttgg
     4381 tgtatggaca atgagtcatt ttatctttgt tcgtccaatg aaaaaagtac aacaacaatc
     4441 cgcaaaggtt aaacagcaaa cgcaaatagt atagaagatt attatgcaaa tgatgttgat
     4501 caaataaaag tgattggaaa aggagaaata attatgatac acgaattagg tacagtagga
     4561 atggtatgtc catttccgtt aattgaagcg caaaagaaaa tggcaacatt gcaatctgga
     4621 gatgaattaa aaattgattt tgattgcacg caagcgacgg aagccattcc aaattgggct
     4681 gcagaaaatg gttatcctat aacaaactat gaacaaattg ataatgcttc atggacaatt
     4741 acaattcaaa aagtttaacg ttatcatttt aacaataaaa tagatattag attctatggc
     4801 tacttccgct aatttaaaag tgagtaagta gtcttttttg ttttagttca tgaaatcatt
     4861 tttatatagt gcggcatatt ttatttcaaa agatgtaata aaacttaacg catttttgct
     4921 ttttataaat tgtcagatta ttatgaaaac aagggagtgg taagtatgaa tcttaacgat
     4981 acgatattta tgtttttgtg tacattatta gtttggttaa tgacaccagg attaagttta
     5041 ttttatggtg ggttagttca atctaaaaat gcgcttaata ctgtcatgca aagtatggca
     5101 gcaattgtgc ttgttacatt tgtatggata acagttggtt ttacaattag ttttgggaat
     5161 gggaatttat ggttcggaaa ttgggaatat acttttctta atcatgtagg ttttgcgact
     5221 caagaagata ttagcccaca tattcctttc gctttgttta tgttatttca aatgatgttt
     5281 tgtacgattg caatttctat tttatctggt tcaatcgctg agaaaatgaa gtttattcct
     5341 tatttattat tcgtagtaat atggactgct cttgtataca gtccagtagc acattgggtt
     5401 tggggcggcg gttggattaa taaactcggt gtattagatt tcgctggagg tacggttgtt
     5461 catattacat caggtgtttc tggtttagta ttagctatta tgattggaaa aggaaacaaa
     5521 cattctgaat caacaccaca taatcttatc attacgttga ttggcggtat attcgtgtgg
     5581 attggttggt atggatttaa tgtaggtagt gcttttacat ttgataatat tgcgatgctt
     5641 gcatttacaa atactgtcat ttcagccagt gcaggtgcta taggttggtt aattttagaa
     5701 tatattttta aaaagacgac aagtttactt ggacttttac tcggtgcatt agcaggatta
     5761 gttgttatta ctcctgcagc aggatatgta acatatctta gtgcaacaat aatggcttta
     5821 ataggaggta tctgttgtta tattgtcatt aattacatca aggtaaaact aaaatatcat
     5881 gatgcattag atgcatttgg tattcatggt gttggtggta ttattggtgc tgttttaaca
     5941 gcagttttcc aaagtaaaaa agccaatcct gacattgaga atggctttat ttatactggt
     6001 gacatacata ttatacttgt acaaatatta tgtgtaacag cagttgtaat ttttagtatc
     6061 gtcatgacgt ttattattgc gaaagtaatt aaattaatta caccattatc tgttacggaa
     6121 caagaaacga atataggatt agacaagatt gttcacggtg aacatgctta ctttgaaggt
     6181 gagctaaata gattcaataa acatattcga tattagaata tatttacata gaatattcat
     6241 tgtcctgaca tttaactaaa ggttgatgtt gggacatttt gttatacaaa agttttattt
     6301 tgaaatcttt ttatgaaaga agcagaaata ttatttaaag cggttacaca tatgctaaaa
     6361 taaggctaag tgtcacaaat aatgataggt gaataagtat gaaaaatata tctgatattg
     6421 ccaaattggc aggcgtttca aaaagcacag tatctagatt tttaaataat ggatctgtca
     6481 gtaaaaaaac aagtgaaaaa ttaacaagaa ttatagcaga acatgactat caaccgaatc
     6541 aatttgctca aagtttaaga gcgagacaaa cacaccttat tggtgccatt atacctagaa
     6601 tgaattcata tgcagtagat gaaacaatca aaggattggc aaaacaatgc caaaaatatg
     6661 aatcacaatt aattttaaat tacacaggtt taaatatcga agcagaaata caagcgcttg
     6721 aaacattagc acgcagtaaa gtagatggta ttgttttaat ggctacagac ataacagaga
     6781 gacatattga agtcattaat aaaatgaatg taccaatcgt tattgttggt caacaacatg
     6841 aacaacttca tagtattgtg catgatgatt ataaagcagg tcaaattata ggcgaatgga
     6901 ttggtcaaca gggatatcaa caagttgaag tgtttagtgt aagtgaaaaa gatattgcag
     6961 ttggtataca tagaaaacgt ggtttacttg accagttagc taaataccaa attaaaccta
     7021 atatacatga aacaaatttt acttatgtgg aagcacaaaa agatgttgca aatgttttgg
     7081 aaaatgtgga gcaagtagat gcggttgttg gagcaactga tacgattgca ttagctgcct
     7141 ataaatatta ttctgataaa aatgatgtta tgaaaccaca tcaaatatat ggttttggtg
     7201 gtgacccaat gacacaatta gtgtctccat cgataaaaac aattcattat aattattttg
     7261 aagctggcca atgcgcgatg gaagagatac aacagatgct taaaaagcaa gatatgccat
     7321 atagcgtcac agtagatgtt aatatttaga cgctgtattt tttaaaacaa atgtggaacc
     7381 gataccatat aactataaat ggataggtta aaagttaaag aacgtaggta aaatttgcta
     7441 taatagaata taaattgtta acagcataaa ttataaaagg aggactgggt aaatattatg
     7501 accgaatgga ctagagaaga acgttatcaa cgaatcgagg acgttgatac tgagtatttt
     7561 aaaacattaa aacaacaagt tgatcaatca aaatttcgtc aacaatttca tatacaacca
     7621 gaaacaggtt tattaaatga tcccaacgga cttatttttt ataaagggaa gtattatgtt
     7681 tcacatcaat ggttcccatt aggcgcagta catggcttaa agtattggta taactacacg
     7741 agtgatgact taataaactt taaacctgaa gggccaatat taaatccaga tactaaatat
     7801 gacagccatg gtgtttatag cggtagcgct tttgaatata acgggcattt atattatatg
     7861 tacacaggaa atcatcgaga taatcattgg caacgacatg cgagtcagat gatcgcacga
     7921 ttgaaagaag acggttcagt tgaaaagttt ccaaagccag taattagcca gcaaccagaa
     7981 ggatatacaa gtcattttag agatcctaaa gtttttaaat atggtgagaa atattatgca
     8041 atcattggcg cacaaaataa tgatcagcaa ggtcgattat tactttataa tactgaagat
     8101 ataattaatt ggcattattt aggtgaaata aatacagagt tagatgattt tggatatatg
     8161 tgggaatgcc cagattactt taatttagat aatcaagatg tcatacttat ttgtccacaa
     8221 ggtattgaac caaaaggcaa tcagttcaaa aatatttatc aaagtggtta tatacttgga
     8281 aagtttgata ttgaaaagtt aacatatgaa catgaaaatt ttgtcgagct tgataatggc
     8341 tttgatttct atgcacctca aacattttta gatgaaaaag gccgacgagt actaattgga
     8401 tggatggggt taccggaaat cgaatatcct actgataatg aaggatgggc ccattgctta
     8461 acaattcctc gtgtattaaa tgtagaaaat ggtcaactta agcaacgtcc atatccagcg
     8521 ttggaaaaat tacgtcacaa taaagagaca gcattaggct acgcaaataa atttactcga
     8581 aaattacatc cgtatgaagg taaacagtat gaattaatca tagatatttt ggataatgat
     8641 gctaccgaag tgtactttga attacgtaca tctaagactt cttcaacatt aattgcttat
     8701 aacaagcgtg aaaataaaat aacattagat cgcagcgaca gtggtttatt gccgacaaat
     8761 gttgaaggta cgacgcgtag tacgatatta gacacgccat taaagcaatt acaaattttt
     8821 gttgatacat ctagtataga aattttctgt aatgatgggg aacgtgtttt gacatctcga
     8881 attttcccga cagaagacgc actaggtatt aaaacatcaa cagaatcagg gcaagtatat
     8941 ttacaattta ctaaatatga tttaaaggat gaacataaat gagacgtcta ttttcaatag
     9001 gagaagcgtt aattgatttt attccaaatg ttacaaatgc gaatttaaaa gatgttcaaa
     9061 catttacgaa gcaaattggt ggcgcgccat gcaatgtagc ttgtacagtt caaaagttag
     9121 gacaacaagc gtatatgatt acacaattag gtaatgatgc atttggagat agcattattg
     9181 aaacgatatc aagtattggt gtagatgtat caaaagtgta tcgcacgaat gaagcaaaca
     9241 ctgcgttagc ttttgtgagt ttgactgaag cgggcgagcg agatttttct ttttatcgta
     9301 agccctctgc tgatatgtta tttgaaccaa gttttgtgaa tgatattgat gtgaatgaaa
     9361 atgatgtagt acatttttgt tctgttgatt tggtcgatag tccgatgaga gatgcacatt
     9421 atcaattgat taccaaaaca ttaaatgcaa atggtactgt cgtatttgat cctaatgtaa
     9481 gattgccatt atgggataac gctgaagatt taagacaaac tattcatacc tttttgccat
     9541 tggcgcatat tgtaaaagtt tcagatgaag aattagaatt tattacaggt attcatgatg
     9601 aaaatgaagc cattcaatca ttatttacag gtaatgttac tgtagtcatt tatacgaagg
     9661 gagcagatgg tgctgccgta tatttgaaaa atggtattaa tcattatcat agtggctata
     9721 aggtaaaacc tgttgataca actggggcag gggatgcttt tattggtgca gttattagca
     9781 ggattttagc aaccgatgtg ttaaatttaa ctcagttatt tgaaaatgaa ggtgaggaaa
     9841 ttttagcgtt tagcaatcgc gttgctgcaa tagtgacaac gaagtatggt gccattaata
     9901 gtttgccaac attacaagag gttgaacaag cattttaaat aattcattta taaaacagtg
     9961 taggaaacaa gttgaatctt gtgctacact gttttttaaa acaaaataag attcacgggg
    10021 taggaaattg tagcatttta tttatggaaa ctgtcaattt caaatttagt atattttgtt
    10081 aaaaattgcg ccataggatt gtagagtgaa atagaaattg ataaattatg tagtaatagg
    10141 aataggtata caaagaaacg ttacatcatt ttttaattca cgcgtcatat ttaattttgt
    10201 tattaaacaa ttaaaaagcc agctatacag tgcatttgct agttatcttg ttaaaatcca
    10261 acaagattta caattgaata cgccgttaac tgactttatt atcttattat atttttttaa
    10321 cgtttctcac cgatgcatag cagtgttctt tatttttaaa atagacaatt cgctctttcg
    10381 aatctataga ttcaatatta tggcgattga cgacaaagct attatgacat ctaaagaaac
    10441 gatcatctaa ttgactcagt tcttttaaat taccataaaa ttcaatttga cggttatcta
    10501 aatgggcaat gagtctgtga gattttgttg atgattcaaa aaacataata tcatcatatt
    10561 gaacatacac tgaattactg ccacgtttta attcaatcgt ttcaacgcta ttatctttag
    10621 acaacaattg taagcgtgta tgtgcagttt ctaaacaatc tataattcga gttcttaatt
    10681 ctgctggatc atctttaaaa ataaaatcca tcgctgcaac tttgtagaca aatgttaaat
    10741 aggtaagttc actgtgactc gtaacgaaaa taatgttacc aactgggtca tgcttacgaa
    10801 tttcactgcc taatttgata ccattaatat cagttgaaag ttgaatatct aaaaagtaac
    10861 agcctatgtc attcatattt ttagcttgct caagcacctc ataaggatta tcagttgcga
    10921 gggcaatttc cataggcttt tcttctatca ttatataatt tttaataatg gtaaccatgt
    10981 tttctctttg ttttggatcg tcttcgcaaa tgaaaatttt catacattca catccttatg
    11041 gctagttgtt aataatttca actttttgaa taaagaaacc attttcgata attgtatcta
    11101 ataagacatt gtctgcatta tcagcaattt cttttaaagt tgatagacct aaaccacgac
    11161 cttcaccttt agtagaaaaa ctttcttgga acaattcatg aatgcgtggt atatcatcag
    11221 cgcatttatt cataacaata aacgttactg aattttcact ttcaataaat gcaacgcgaa
    11281 tgatagggtc atcaatttca gttgatgcct caattgcatt atcaagaata ataccaatac
    11341 tgcgacttaa atcgatcata ttcaagttaa tgctacttac ttcatcgggt atttcgatac
    11401 taattggaat attcatttct tgtgcacgta aaattttcgc agtaattaag ccttttatct
    11461 cacgtacttt aagattctcg ataccattta atttaatagc attcatctgc aaattatctt
    11521 tcataggtac aatattttta ttgaaataag cgcgtagacc aatcatgtca tcttcgcgaa
    11581 tgtattctga aagtgtcgtt aagatattga cataatcatg acggaacttg cgcatttcat
    11641 tattaattgc ttcaatcttt aatgtgtatt cgtaataggt ctcaatttct tcttgatttc
    11701 gtttgtattt catctcttta agggtgaaaa gcgacattat aagtattaca acagagataa
    11761 aaattattaa tcctataaat acaagtgcat atggaattaa gctatcaccg tttgaacttg
    11821 tatttgtaga ataaatataa aaaaatgcaa atgttgcaaa taatacgaat gaaatta
//
LOCUS       DAHHSC010000061        11815 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.061, whole genome shotgun sequence.
ACCESSION   DAHHSC010000061 DAHHSC010000000
VERSION     DAHHSC010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 11815)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11815)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11815
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.061"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            complement(129..326)
                     /locus_tag="NBI57_001284"
     CDS             complement(129..326)
                     /locus_tag="NBI57_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1822239.1"
                     /translation="MNCYDEIFNTIKKLIENKEISSYQINKDTGISYGNINAMRRGER
                     RIENLSLKNAKILYEYAKKVL"
     gene            complement(819..2159)
                     /gene="glnA"
                     /locus_tag="NBI57_001285"
     CDS             complement(819..2159)
                     /gene="glnA"
                     /locus_tag="NBI57_001285"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HCD1822240.1"
                     /translation="MPKRTFTKEDIRKFAEEENVRYLRLQFTDILGTIKNVEVPVSQL
                     EKVLDNEMMFDGSSIEGFVRIEESDMYLHPDLDTWVIFPWTAGQGKVARLICDVYKTD
                     GTPFEGDPRANLKRVLKEMEDLGFTDFNLGPEPEFFLFKLDEKGEPTLELNDDGGYFD
                     LAPTDLGENCRRDIVLELEDMGFDIEASHHEVAPGQHEIDFKYADAVTACDNIQTFKL
                     VVKTIARKHNLHATFMPKPLFGVNGSGMHFNVSLFKGKENAFFDPNTEMGLTETAYQF
                     TAGVLKNARGFTAVCNPLVNSYKRLVPGYEAPCYIAWSGKNRSPLIRVPSSRGLSTRI
                     EVRSVDPAANPYMALAAILEAGLDGIKNKLKVPEPVNQNIYEMNREEREAVGIQDLPS
                     TLYTALKAMRENEVIKKALGNHIYNQFINSKSIEWDYYRTQVSEWERDQYMKQY"
     gene            complement(2178..2546)
                     /locus_tag="NBI57_001286"
     CDS             complement(2178..2546)
                     /locus_tag="NBI57_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HCD1822241.1"
                     /translation="MISNDAIRRNMAVFSMSVVSKLTDLTPRQIRYYETHELIKPERT
                     EGQKRLFSLNDLERLLEIKSLLEKGFNIKGIKQIIYDSQEHLTTDEQEIRKKMIVDAT
                     QKPIGETLPINRGDLSRFIK"
     gene            complement(2789..4027)
                     /locus_tag="NBI57_001287"
     CDS             complement(2789..4027)
                     /locus_tag="NBI57_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HCD1822242.1"
                     /translation="MKDISKIVADVESTLAPYFKEIEETAYINQEKVLNAFHHVKATE
                     SDLQGSTGYGYDDFGRDHLEEIYAQAFKAEDAIVRPQIISGTHAITIALQSLLKHGDE
                     LIYITGSPYDTLLEVIGVNGNGIESLMEHGVSYKDIALKEGKIDIESVLDGVSERTKV
                     IAIQRSKGYDQRPSIPLDEIEKVITRLKNVHPNILIFVDNCYGEFVERREPIECGADL
                     IAGSLIKNPGGGLAKIGGYIAGRKDLIERCGYRLTAPGIGKEAGASLNALLEMYQGFF
                     LAPHVVSQSLKGALFTSLFLEKMNMNTTPKYYEKRTDLIQTVKFETKEQMISFCQSIQ
                     HASPINAHFSPEPSYMPGYEDDVIMAAGTFIQGSSIELSADGPIRPPYEAYVQGGLTY
                     EHVKIAVTRAVNQLKEQGLI"
     gene            complement(4046..5284)
                     /gene="hflX"
                     /locus_tag="NBI57_001288"
     CDS             complement(4046..5284)
                     /gene="hflX"
                     /locus_tag="NBI57_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HCD1822243.1"
                     /translation="MAQQQIHDTKDKLEKAVLVGVHAQDDKQFNFESTMEELSSLSET
                     CQLEVLGQITQNRDRVDRKYYVGKGKIEEIQAFIEFKDIDVVITNDELTTAQSKSLNE
                     ALGVKIIDRTQLILEIFALRARSKEGKLQVELAQLDYLLPRLQGHGKSLSRLGGGIGT
                     RGPGETKLEMDRRHIRTRMNEIKHQLRTVEEHRERYRNKRNQNQVFQVALVGYTNAGK
                     SSWFNVLANEETYEKDQLFATLDPKTRQIQINDGFNLIISDTVGFIQKLPTTLIAAFK
                     STLEEAKGADLLVHVVDSSHPEYRTQYDTVNDLIKQLDMSHISQIVIFNKKDLCDHAS
                     NRPASDLPNVFVSSKNDGDKLLVKTLFIDEIKRQLTYYDETIATNNADRLYFLKQHTL
                     VTELKYDEIENVYRIKGFKK"
     gene            5396..5872
                     /locus_tag="NBI57_001289"
     CDS             5396..5872
                     /locus_tag="NBI57_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HCD1822244.1"
                     /translation="METIYDFVVETNKGVTYKLDAYKGDVMLIVNTASECGFTSQFEG
                     LQSLYEKYKDQGFVILGFPCNQFGGQEPGSGEEAAQNCKLNYGVTFPMHQKIDVKGEH
                     QLPLFRYLTAAQHGFFNEKIKWNFTKFLVDREGNVVKRFAPQKKPVQIEREIEKLL"
     gene            complement(6094..6327)
                     /gene="hfq"
                     /locus_tag="NBI57_001290"
     CDS             complement(6094..6327)
                     /gene="hfq"
                     /locus_tag="NBI57_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HCD1822245.1"
                     /translation="MIANENIQDKALENFKANQTEVTVFFLNGFQMKGVIEEYDKYVV
                     SLNSQGKQHLIYKHAISTYTVETEGQASTESEE"
     gene            complement(6342..7277)
                     /gene="miaA"
                     /locus_tag="NBI57_001291"
     CDS             complement(6342..7277)
                     /gene="miaA"
                     /locus_tag="NBI57_001291"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HCD1822246.1"
                     /translation="MNKNKPFIVVIVGPTASGKTELSIELAKRINGEIISGDSMQVYK
                     HMNIGTAKVTPEEMDGIPHHLIDILNPDDTFSAYEFKRLAEDLITDITNRGKVPIIAG
                     GTGLYIQSLIYNYELEDETVTPAQLSIVKQKLSALEHLDNQQLHDYLAQFDAVSAENI
                     HPNNRQRVLRAIEYYLKTKKLLSNRKKVQQFTENYDTLLLGIEMSRKTLYSRINKRVD
                     IMLDHGLFREVQQLVEQGYESCQSMQAIGYKELIPVINGQMIYEDAVNDLKQHSRQYA
                     KRQMTWFKNKMSVHWLDKENMSLQMMLDEITTQIK"
     gene            complement(7295..8209)
                     /locus_tag="NBI57_001292"
     CDS             complement(7295..8209)
                     /locus_tag="NBI57_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCD1822247.1"
                     /translation="MTEKQFKLTVQDNTNIEVKVNFTDVDSKGIIHIFHGMAEHMERY
                     DKLAHALSKHGFDVIRHNHRGHGINIDESTRGHYDDMKRVIGDAFEVAQTVRGNVDKP
                     YIIIGHSMGSVIARLFVETYPQYVDGLILSGTGMYSLWKGLPTVKVLQLITKIYGAEK
                     RVEWVNQLVSNSFNKKIRPLRTQSDWISSNPIEVDNFIKDPYSGFNVSNQLLYQTAYY
                     MLHTSQLKNMKMLNHAMPILLVSGYDDSLGDYGKGILKLANIYRKAGIKNVKVNLYHH
                     KRHEVLFEKDHDKIWEDLFKWLNQFYKK"
     gene            complement(8359..10032)
                     /locus_tag="NBI57_001293"
     CDS             complement(8359..10032)
                     /locus_tag="NBI57_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase/oxidase"
                     /protein_id="HCD1822248.1"
                     /translation="MALSTFKREHIKKNLRNDEYDLVIIGGGITGAGIALDASERGMK
                     VALVEMQDFAQGTSSRSTKLVHGGLRYLKQFQIGVVAETGKERAIVYENGPHVTTPEW
                     MLLPMHKGGTFGKFSTSIGLGMYDRLAGVKKSERKKMLSKKETLAKEPLVKKEGLKGG
                     GYYVEYRTDDARLTIEVMKRAAEKGAEIINYTKSEHFTYDKNQQVNGVKVIDKLTNEN
                     YTIKAKKVVNAAGPWVDDVRSGDYARNNKKLRLTKGVHVVIDQSKFPLGQAVYFDTEK
                     DGRMIFAIPREGKAYVGTTDTFYDNIKSSPLTTQEDRDYLIDAINYMFPSVNVTDEDI
                     ESTWAGIRPLIYEEGKDPSEISRKDEIWEGKSGLLTIAGGKLTGYRHMAQDIVDLVSK
                     RLKKDYGLTFSPCNTKGLAISGGDVGGSKNFDAFVEQKVDVAKGFGIDEDVARRLASK
                     YGSNVDELFNIAQTSQYHDSKLPLEIYVELVYSIQQEMVYKPNDFLVRRSGKMYFNIK
                     DVLDYKDAVIDIMADMLDYSPAQIEAYTEEVEQAIKEAQHGNNQPAVKE"
     gene            complement(10190..11686)
                     /gene="glpK"
                     /locus_tag="NBI57_001294"
     CDS             complement(10190..11686)
                     /gene="glpK"
                     /locus_tag="NBI57_001294"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HCD1822249.1"
                     /translation="MEKYILSIDQGTTSSRAILFNQKGEIAGVAQREFKQYFPQSGWV
                     EHDANEIWTSVLAVMTEVINENDVRADQIAGIGITNQRETTVVWDKHTGRPIYHAIVW
                     QSRQTQSICSELKQQGYEQTFRDKTGLLLDPYFAGTKVKWILDNVEGAREKAENGDLL
                     FGTIDTWLVWKLSGKAAHITDYSNASRTLMFNIHDLEWDDELLELLTVPKNMLPEVKP
                     SSEIYGKTIDYHFYGQEVPIAGVAGDQQAALFGQACFECGDVKNTYGTGGFMLMNTGD
                     KAVKSESGLLTTIAYGIDGKVNYALEGSIFVSGSAIQWLRDGLRMINSAPQSESYATR
                     VDSTEGVYVVPAFVGLGTPYWDSEARGAIFGLTRGTEKEHFIRATLESLCYQTRDVME
                     AMSKDSGIDVQSLRVDGGAVKNNFIMQFQADIVNTSVERPEIQETTALGAAFLAGLAV
                     GFWESKDDIAKNWKLEEKFDPKMDEGEREKLYRGWKKAVEATQVFKTE"
BASE COUNT         3614 a         2262 c         1626 g         4313 t
ORIGIN      
        1 ttaaaaaaca taagtaaatc actaaacatg atttctttca cttttagaaa acttttaata
       61 ctataaaaac acccactcag tcactagttt gggcagttat tgtatgccta ttgaactcaa
      121 tgcttatatt acaatacctt tttcgcatat tcatataaga tctttgcatt ctttaagctt
      181 aaattttcta ttcttctttc cccacggcgc atagcattaa tattaccgta acttatccca
      241 gtatctttat taatttgata acttgatatc tctttgtttt ctatcaattt tttgattgta
      301 ttgaatattt catcatagca attcataaat tagatgaggc gaaattttta attttttaga
      361 atatcaatag tagtataact aaaatgaaaa taccgatcga taaacaaaaa gatatttttt
      421 gttttgtttc tcttttcata tagtattacc cccttaataa tgcgtagtaa ggtccctctt
      481 ttcggggtct taccttagaa cgttctgcaa atgaattcga tgagaagtaa tatgaatatg
      541 gctattttca agtaatactc aacgttttcg cgacgttctt ttatcgcctc atctcatcac
      601 ctccaaatat attaaaattc atgtgaacta aaatataaaa tggtcttccc cagctttaaa
      661 aaaataaata cataaaacat ttacttgacc aaaacttgac caaatccatt ttttaacaaa
      721 agtggtcaag tgaaatccca tctttccaaa tcaaaaaaga gctaaagcaa aacgctctag
      781 cccttgatat tactgatttc ccatcattgt ttagcgtatt aatattgctt catgtactga
      841 tctctttccc attcagatac ttgagttctg tagtaatccc attcaattga ttttgaatta
      901 ataaattgat tatagatatg atttcctaaa gcttttttaa taacttcatt ttcacgcatt
      961 gcttttaatg cagtataaag tgttgaaggt aagtcttgaa tgcctactgc ttcacgttct
     1021 tcacggttca tttcgtaaat attttggtta actggttctg gaacttttaa tttattttta
     1081 ataccatcta gtccagcttc taagattgca gctaacgcca tgtatgggtt tgcagctgga
     1141 tctactgaac gtacttcgat acgagtagat aatcctcttg aagatggtac acggattaat
     1201 ggtgaacggt ttttaccact ccatgcaata taacatggtg cttcataacc aggtactaaa
     1261 cgtttatatg agtttactaa cgggttacat acagcagtaa atccgcgtgc atttttaagc
     1321 acacctgctg taaattgata tgcagtttcc gttaagccca tttcagtatt tggatcaaag
     1381 aatgcatttt ctttaccttt gaataatgaa acgttaaagt gcataccgct accattcaca
     1441 ccgaataatg gtttaggcat aaatgttgcg tgtaaattat gtttacgtgc gattgtttta
     1501 acaaccaatt taaatgtttg gatattatca catgctgtaa cagcatctgc atatttaaag
     1561 tcaatttcat gttgaccagg ggcaacttca tggtgactag cttcaatatc gaagcccata
     1621 tcctctaatt ctaaaacaat atcacgacga cagttttcac ctaaatctgt aggtgctaaa
     1681 tcgaaatatc caccatcatc attaagttct aaagttggtt cccctttttc atccaactta
     1741 aacaagaaga attctggttc aggccctagg ttaaagtctg tgaagcctaa atcttccatt
     1801 tcttttaata cacgttttaa gtttgcacga ggatcccctt caaatggtgt tccatctgtt
     1861 ttatatacat cacaaattaa acgtgcaact ttaccttgtc cagcagtcca tgggaagatt
     1921 acccaagtat ctaaatctgg atgtaagtac atatctgatt cttcgatacg tacgaaacct
     1981 tcgatagaag aaccgtcaaa catcatttcg ttatcaagta ctttttctaa ttggcttaca
     2041 ggcacttcaa catttttaat tgttcctaaa atatcagtga attgtaatct taaatatctt
     2101 acattttcct cttctgcaaa tttacgaatg tcttctttag tgaaagtacg ttttggcatt
     2161 ttaaaatcct ccaaatttta tttaataaat cgggataaat caccacgatt tattggcaaa
     2221 gtttctccaa taggcttttg cgtggcatct acaatcatct tttttcttat ctcttgttca
     2281 tctgttgtta aatgctcttg tgagtcataa atgatttgtt taatcccttt gatattaaat
     2341 cctttttcta ataatgattt aatttctagt aatctttcca aatcattgag tgagaacaga
     2401 cgtttttgac cttctgttct ttcaggtttg atgagttcat gtgtttcata gtaacgtatt
     2461 tgccttggcg ttaaatccgt taacttactt actacactca tagagaagac agccatattt
     2521 cgtctgattg catcattcga tatcattgtt cctctcctct acttttgaac ttgttaataa
     2581 cttatcataa tttttttagg atttcaaaaa tatgtcaggt tttctaacat aaagcttaaa
     2641 acattgatat aactgcgatt tcgacttcta aaacgacata aaacaatcta caactaaaat
     2701 attttttcaa tttttaaaaa taatctgata aatctccgca aaaaatttca cccaagacac
     2761 attatcattt agggtgaaat ttaatctatt atataagtcc ttgttctttc aactgattaa
     2821 cagctcttgt cacagcaatt ttaacgtgtt catatgttaa tcctccttga acatatgctt
     2881 cataaggagg acgaataggt ccatctgcag ataattcaat cgatgaacct tgaataaacg
     2941 taccagctgc cataataaca tcatcttcgt aaccaggcat ataactaggt tctggactaa
     3001 aatgtgcatt aattggggat gcgtgttgaa tactttgaca aaatgaaatc atttgttctt
     3061 tcgtttcaaa tttaactgtt tgaattaaat cagttcgttt ttcgtagtac ttcggcgttg
     3121 tgttcatatt cattttttct aaaaataaac tagtaaacaa tgcaccttta agactctgac
     3181 tgacaacgtg tggtgctaaa aagaaacctt gatacatttc aagcaatgca tttaatgatg
     3241 cacccgcttc tttaccaata ccaggtgctg tcaatctata accacatcgt tcaattaaat
     3301 cttttctacc agcaatgtat ccaccaatct tagctaaacc accgccaggg tttttaatta
     3361 atgatcctgc tattaaatcg gcaccacatt ctataggttc acgtctttca acaaattccc
     3421 cataacagtt atccacaaat attaaaatat taggatgcac gtttttcaac ctagtaatta
     3481 ccttttcaat ttcatctagc ggaattgaag gtctttgatc atagcctttc gaacgttgaa
     3541 tcgctattac tttggtgcgc tcagaaaccc catctaacac actttcaata tcgatcttac
     3601 cttctttaag tgcaatatct ttatacgata cgccgtgctc cattaaactt tcaataccat
     3661 ttccgtttac gccaatgact tcaagtaaag tgtcatatgg actacccgtt atataaatta
     3721 attcatcacc atgttttaat aaactttgta atgcaatagt aatcgcatgc gtacctgaaa
     3781 taatttgcgg acgaacaatt gcatcttctg ctttaaatgc ctgcgcatat atttcttcta
     3841 aatgatcacg tccaaagtca tcatacccgt atcctgttga tccttgtaga tcactttcgg
     3901 ttgctttgac atgatgaaat gcatttaata ctttttcttg attaatatat gctgtttctt
     3961 caatttcttt aaaatatggt gctaacgttg attcgacgtc agctactatc ttacttatat
     4021 ctttcatttg aatttcgtcc ttttattatt ttttaaatcc ttttatacga taaacatttt
     4081 caatttcatc atatttaagt tcagtcacta atgtatgttg ttttagaaaa tataatcgat
     4141 ctgcattatt cgtcgcaatt gtctcatcat aataagttaa ttgccttttg atttcatcaa
     4201 taaataacgt cttaacaagt aatttatcac catcattttt agaagaaaca aaaacattag
     4261 gcaaatcact tgctggacga tttgatgcat gatcacataa gtccttttta ttaaaaataa
     4321 ctatttgaga aatatgactc atatctaatt gtttgattaa atcattaact gtgtcatact
     4381 gcgtacggta ttcaggatgg ctactatcta cgacatgtac taataaatct gcacctttag
     4441 cctcttctaa agttgattta aaagctgcaa ttaacgtcgt aggtagtttc tgtataaaac
     4501 caacagtatc tgaaataatt aaattaaatc catcatttat ttgaatttgt cgtgttttag
     4561 gatctaacgt tgcaaataat tgatcttttt catacgtctc ttcatttgct aaaacattaa
     4621 accatgatga tttaccagca tttgtataac caactaaagc tacttgaaac acctgatttt
     4681 gatttctttt atttcgatat ctttcgcgat gttcttctac cgtccgcaat tgatgtttaa
     4741 tttcattcat acgagttcga atatgtctgc gatccatctc taacttcgtt tcaccagggc
     4801 ctctagttcc aataccgcca cctaaacgag aaagactttt accatggcct tgcaatctag
     4861 gtaataaata atcaagttgt gctagctcta cttgcaattt accttcttta cttcttgctc
     4921 ttaatgcaaa tatttcaaga atcaactgag ttctatcaat aatttttaca cctaaagctt
     4981 catttagtga tttggattgt gcagtcgtta attcatcatt tgtgatgact acatcaatat
     5041 ctttgaactc aataaatgct tgaatttctt caattttacc tttaccaaca taatatttgc
     5101 gatctacacg atctctgttt tgagtaattt gacccaacac ttcaagttgg caagtctctg
     5161 ataaagatga taattcctcc attgtagact caaaattaaa ttgcttatca tcttgagcat
     5221 gtacaccgac taagacagct ttttctagtt tatctttagt atcatgaatt tgttgctgag
     5281 ccatgttata catctcctta ataaaatcct actcaaagta ttctatcata gagttttagt
     5341 aaagactatt acacgatttt atggtacatt ttaagtaaat cgaaaggatg attcaatgga
     5401 gacaatttat gattttgtag tagaaacaaa taaaggtgtt acttacaaat tagatgcata
     5461 taagggtgac gttatgctaa ttgttaatac agctagcgaa tgtggcttta cttcacaatt
     5521 tgaaggtttg caatcattat acgagaaata caaagatcaa gggtttgtaa ttctaggttt
     5581 cccttgtaat caatttggtg gtcaagaacc tggatcaggc gaagaagcag ctcaaaactg
     5641 taaattaaac tatggtgtta ccttccctat gcatcaaaaa atagacgtaa aaggggaaca
     5701 tcaattacct ttatttagat atttgactgc ggcacaacac ggattcttta atgagaaaat
     5761 caaatggaat ttcactaagt ttttagtgga tcgcgaaggt aacgttgtta aacgatttgc
     5821 acctcaaaaa aaacctgttc aaatcgaaag agaaatagaa aaattattat aaaatatact
     5881 agctcatagc aaataaaacg cttcatataa agtcgtatca tatattgact cgacctttat
     5941 atgaagcgtt tttattacaa acattcctct attgtatctt gtcatttgaa catcatttaa
     6001 ttgatgtcac atatgacttt atttctatat ctatttatca tacttcaaac gttatgtaca
     6061 cgagtcatta aatcgtgccc atatgattac aacttattct tcactttcag tagatgcttg
     6121 accttcagtt tctactgtat aagtgctgat cgcatgtttg taaatcaagt gttgtttgcc
     6181 ttgagaattt aagcttacga catacttgtc gtattcttca ataacacctt tcatttggaa
     6241 accgtttaga aagaatacag ttacttcagt ttggtttgct ttaaaattct ctagtgcttt
     6301 gtcttggatg ttttcgtttg caatcatctg tcggactcct tttacttaat ctgggttgta
     6361 atctcatcta acatcatttg aagtgacata ttttctttat ctaaccaatg aacactcatt
     6421 ttattcttga accatgtcat ttgtcgtttt gcatattggc gtgaatgttg ctttaaatca
     6481 ttgacagcat cttcataaat catttgtccg ttaatcacag gtattaattc tttatatcca
     6541 atagcttgca tactttggca agattcatag ccttgttcaa caagttgttg cacttctcta
     6601 aataatccgt gatccaacat aatatcaaca cgtttattta ttcttgaata taatgtttta
     6661 cgcgacattt caatccctaa taataatgta tcataatttt cagtaaattg ttgcactttc
     6721 ttgcgattac tcaaaagttt ttttgttttt aaataatatt caatagcgcg caacactctt
     6781 tggcggttgt tagggtgaat attttctgca gaaaccgcat caaattgagc taaataatcg
     6841 tgtagttgct gattatctaa atgttctaat gcagataact tttgtttaac tatggataat
     6901 tgtgcaggtg taactgtttc atcttctaat tcataattat atattaatga ttgaatatat
     6961 aagcctgttc cacctgctat gattggaact ttacctctat tcgttatatc agtaattaaa
     7021 tcttctgcta atcgcttgaa ttcatatgct gaaaatgtat catcaggatt caagatatca
     7081 attaaatgat gtggaatacc atccatttct tcaggtgtta cttttgcagt tccaatattc
     7141 atatgtttgt aaacttgcat agaatcgccg cttatgattt caccattgat acgcttcgcg
     7201 agttctatgc taagctctgt tttacctgaa gcagttggcc ccacaattac tacaataaaa
     7261 ggcttatttt tattcatatt taattccact ttctttattt tttataaaat tgattcaacc
     7321 atttaaacaa gtcttcccaa attttgtcat gatctttttc aaataacact tcatgacgtt
     7381 tatgatgata aagattcact ttaacatttt taatgccagc ttttctatat atattcgcca
     7441 attttaaaat ccctttacca taatcaccta aagaatcgtc atatcctgaa actaataata
     7501 taggcatggc atgatttaac attttcatat tttttaattg tgatgtatgt agcatataat
     7561 aggctgtttg atataataat tgatttgaca cattaaatcc actatatgga tctttaataa
     7621 agttatctac ttcaattgga ttactagaaa tccaatcact ttgtgtacgt aatggacgta
     7681 tttttttatt aaaactattt gatactaact ggttaaccca ttcaactcgt ttctcagcac
     7741 cataaatttt tgtaatcagt tgtaacactt taacggttgg taaacctttc cataatgaat
     7801 acataccagt accacttaaa attagaccat caacatattg cggatatgtt tctacaaaca
     7861 atctagctat aacggatccc attgaatgtc cgattataat gtatggttta tcaacattgc
     7921 ctctcactgt ttgcgctact tcaaaggcat caccgataac tcgtttcata tcatcgtaat
     7981 gccctcttgt tgattcatca atattaatac catgtcctcg atgattatga cgtatcacat
     8041 cgaagccatg ctttgaaagt gcatgtgcta atttatcgta acgttccata tgttcagcca
     8101 taccatgaaa tatatgaata attccttttg aatctacatc tgtaaaattc actttaactt
     8161 caatattcgt attatcttgt acagttaatt taaattgttt ttcagtcatc atctcacacc
     8221 tttaataaca acgtttttat attcgctttt attattatca tacatttagt gatgcatagt
     8281 ttcaattgac taatgtatct cgtataaaaa aactgatgcc cttaaaacag gacaccagtt
     8341 tatgattgta caaattaatt attctttaac tgctggttga ttatttccat gttgcgcttc
     8401 tttaattgct tgctcaactt cttcagtata tgcttcaatt tgagctggag agtaatcaag
     8461 catatctgcc ataatatcga tgacagcatc tttataatct aatacatctt taatattgaa
     8521 atacatttta ccagaacgac gaactaagaa atcgttaggt ttgtatacca tttcttgttg
     8581 aatactataa acaagttcta cataaatttc taatggtaac ttgctatcat ggtattgaga
     8641 tgtttgcgca atgttgaaca attcatcaac atttgaacca tatttagatg ctaaacgtct
     8701 tgcaacatct tcatcaatgc cgaatccttt agctacatct actttttgct ctacaaacgc
     8761 atcaaagttc ttgctaccac ctacatcgcc acctgaaatt gccagacctt ttgtattaca
     8821 tggactaaat gttaaaccgt agtctttttt caagcgttta gatactaaat caacaatgtc
     8881 ttgagccatg tgacgatagc ctgttaattt accacctgca atagttaata aacctgattt
     8941 accttcccaa atttcatcct tacgagagat ttcagaaggg tctttgcctt cttcgtaaat
     9001 taatggtcta attcctgccc atgttgattc aatatcttca tctgtaacat taacactagg
     9061 gaacatgtaa ttaatcgcat cgattaaata gtctctgtct tcttgtgtag ttaatggtga
     9121 agatttgata ttgtcataga atgtatctgt agtacctaca tacgcttttc cttcacgtgg
     9181 aattgcaaaa atcattcttc catctttttc agtatcaaag tatactgctt gacctaatgg
     9241 gaattttgat tgatcaataa caacatgtac acctttagtt aaacgtaatt ttttattatt
     9301 gcgtgcataa tcaccacttc taacatcatc aacccatggg cctgctgcat taaccacttt
     9361 tttagcctta attgtataat tttcattagt taatttatct ataactttaa caccatttac
     9421 ttgttgattt ttatcataag tgaagtgttc agatttagta tagttgataa tttctgcgcc
     9481 tttttcagca gcacgcttca taacttcaat agttaaacgc gcatcgtcag tacgatattc
     9541 aacatagtaa ccgccgcctt ttagaccttc ttttttaact aatggttctt tagctaaagt
     9601 ttctttttta gataacattt ttttacgttc agacttctta acacctgcta aacgatcata
     9661 cattcctaaa ccaattgatg ttgagaattt accaaatgtt ccacctttat gcattggtaa
     9721 aagcatccac tctggagtcg taacatgagg cccattttca taaacaatcg cacgttcttt
     9781 accagtttcg gcaactactc caatttggaa ttgttttaag taacgtaaac caccatggac
     9841 taattttgta gatcttgagc ttgttccttg tgcaaagtct tgcatttcaa ctaatgcaac
     9901 tttcattcct ctttcactcg cgtctagtgc aatacctgca cctgtaatac cgccaccaat
     9961 aattactaaa tcatattcat catttcttaa attcttttta atatgttctc tcttaaaagt
    10021 agacaatgcc attccaaaac gcctcctaaa aaatgtaaaa acgagagact tgtcccacat
    10081 aaacaatcct tcaattgtac atgttcgaac aaatctctcg tttctcgatt caaatattaa
    10141 cttaaattga ttgtatcaca atgtttatac aaaagtctaa tctacaagtt tattctgttt
    10201 taaaaacttg tgttgcttca acagcttttt tccaacctct atataatttt tctctttcgc
    10261 cttcatccat tttcggatcg aatttttctt ctaatttcca gtttttagcg atatcatctt
    10321 tactctccca gaatccaact gctaaacctg ctaaaaatgc agcacctaaa gctgtagttt
    10381 cttgaatttc aggtctttca acagaagtat taacaatgtc tgcttggaac tgcataataa
    10441 agttattttt aactgcacca ccatcgacac gtaaactttg gacatcaata ccagagtctt
    10501 ttgacattgc ttccataacg tcacgagttt ggtaacataa tgattctaaa gttgcacgga
    10561 taaagtgctc tttttcagtt ccacgtgtta aaccgaaaat cgcaccacgt gcttcagaat
    10621 cccaatatgg tgttcctaaa cctacaaaag ctggaacaac ataaacaccc tcagtagagt
    10681 caactcgtgt cgcataactt tctgattgtg gtgctgaatt aatcattctt aatccatcac
    10741 gtaaccattg gattgctgaa cccgaaacaa agatggaacc ttcaagcgca taatttactt
    10801 ttccatcaat accataagca attgttgtta ataaaccact ttcagattta accgctttgt
    10861 cacctgtatt cattaacatg aagccaccag ttccatatgt gtttttcacg tcaccacatt
    10921 cgaagcaagc ttgtccaaat aatgctgctt gttgatcacc agctacacca gcgattggta
    10981 cttcttgacc atagaagtgg taatcaattg tcttaccata tatttcactt gaaggtttaa
    11041 cttctggcaa catattttta ggtactgtaa gtagttctaa taactcatcg tcccattcta
    11101 aatcatggat attaaacatt aatgtacgac tcgcatttga ataatcagta atatgcgcag
    11161 cttttcctga taatttccat actaaccaag tatcaatcgt tccaaataat agatcgccat
    11221 tttctgcttt ttctcgtgca ccttcaacat tgtctagaat ccatttaact tttgtacctg
    11281 caaaatacgg atctaaaagt aatcctgtct tatctctaaa tgtttgttca tatccttgtt
    11341 gttttaattc tgaacaaatt gattgtgttt gacgtgattg ccaaacaatt gcgtgataaa
    11401 ttgggcggcc agtatgtttg tcccaaacaa ccgttgtttc acgttggttt gtaataccga
    11461 ttcctgcaat ttgatcagct ctaacatcat tttcattaat tacttccgtc attacagcta
    11521 acacagatgt ccaaatttca tttgcatcat gttcaaccca acctgattgt ggaaaatatt
    11581 gcttaaactc acgttgtgct acccctgcaa tttccccttt ttgattgaat aaaatcgctc
    11641 ttgagcttgt tgttccttgg tctatagata aaatatattt ttccattagt agtatctcct
    11701 ttattaaaaa ataatcgcta acccattgta caaaatctgt taaattttgg cgaaaaaata
    11761 gcataatcca tgaatcaaac atgttaaatt atgctattta cgtttctttt tattt
//
LOCUS       DAHHSC010000062        11742 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.062, whole genome shotgun sequence.
ACCESSION   DAHHSC010000062 DAHHSC010000000
VERSION     DAHHSC010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 11742)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11742)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11742
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.062"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            294..1982
                     /gene="ilvD"
                     /locus_tag="NBI57_001295"
     CDS             294..1982
                     /gene="ilvD"
                     /locus_tag="NBI57_001295"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HCD1822250.1"
                     /translation="MRSDMIKKGDHQAPARSLLHATGALKSPTDMNKPFVAICNSYID
                     IVPGHVHLRELADIAKEAIREAGAIPFEFNTIGVDDGIAMGHIGMRYSLPSREIIADA
                     AETVINAHWFDGVFYIPNCDKITPGMILAAMRTNVPAIFCSGGPMKAGLSAHGKALTL
                     SSMFEAVGAFKEGSISKEEFLDMEQNACPTCGSCAGMFTANSMNCLMEVLGLALPYNG
                     TALAVSDQRREMIRQAAFKLVENIKNDLKPRDIVTREAIDDAFALDMAMGGSTNTVLH
                     TLAIANEAGIDYDLERINAIAKRTPYLSKIAPSSSYSMHDVHEAGGVPAIINELMKKD
                     GTLHPDRITVTGKTLRENNEGKEIKNFDVIHPLDAPYDAQGGLSILFGNIAPKGAVIK
                     VGGVDPSIKTFTGKAICFNSHDEAVEAIDNRTVRAGHVVVIRYEGPKGGPGMPEMLAP
                     TSSIVGRGLGKDVALITDGRFSGATRGIAVGHISPEAASGGPIALIEDGDEITIDLTN
                     RTLNVNQPEDVLARRRESLTPFKAKVKTGYLARYTALVTSANTGGVMQVPENLI"
     gene            2010..3779
                     /gene="ilvB"
                     /locus_tag="NBI57_001296"
     CDS             2010..3779
                     /gene="ilvB"
                     /locus_tag="NBI57_001296"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HCD1822251.1"
                     /translation="MSKTQHEVNQNIDPLKMAESLELEQLNEKTLNDMRSGSEVLVEA
                     LLKENVDYLFGYPGGAVLPLYDTFYDGKIKHILARHEQGAVHAAEGYARVSGKTGVVV
                     VTSGPGATNVMTGITDAHCDSLPLVVFTGQVATPGIGKDAFQEADILSMTSPITKQNY
                     QVKRVEDIPKIVHEAFHVANSGRKGPVVIDFPKDMGVLATNVDLCDEINIPGYEVVTE
                     PENKDIDTFISLLKEAKKPVVLAGAGINQSKSNQLLTQFVNKHQIPTVTTLLGLGAVP
                     YEDTLFLGMGGMHGSYASNMALTECDLLINLGSRFDDRLASKPDAFAPNAKIVHVDID
                     PSEINKVIHVDLGIIADCKRFLECLNDKNVETIEHSDWVKHCQNNKQKHPFKLGEEDQ
                     VFCKPQQTIEYIGKITNGEAIVTTDVGQHQMWAAQFYPFKNHGQWVTSGGLGTMGFGI
                     PSSIGAKLANPDKTVVCFVGDGGFQMTNQEMALLPEYGLDVKIVLINNGTLGMVKQWQ
                     DKFFNQRFSHSVFNGQPDFMKMAEAYGVKGFLIDKPEQLEEQLDAAFAYQGPVLIEVR
                     ISPTEAVTPMVPSGKSNHEMEGL"
     gene            3779..4033
                     /locus_tag="NBI57_001297"
     CDS             3779..4033
                     /locus_tag="NBI57_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HCD1822252.1"
                     /translation="MTRILKLQVADQVSTLNRITSAFVRLQYNIDTLHVTHSEQPGIS
                     NMEIQVDIQDDTSLHILIKKLKQQINVLTVECYDLVDNEA"
     gene            4155..5174
                     /gene="ilvC"
                     /locus_tag="NBI57_001298"
     CDS             4155..5174
                     /gene="ilvC"
                     /locus_tag="NBI57_001298"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001825891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HCD1822253.1"
                     /translation="MEEFIMTTVYYDQDVKTDALQGKKIAVVGYGSQGHAHAQNLKDN
                     GYDVVIGIRPGRSFDKAKEDGFDVFPVAEAVKQADVIMVLLPDEIQGDVYKNEIEPNL
                     EKHNALAFAHGFNIHFGVIQPPADVDVFLVAPKGPGHLVRRTFVEGSAVPSLFGIQQD
                     ASGQARNIALSYAKGIGATRAGVIETTFKEETETDLFGEQAVLCGGVSKLIQSGFETL
                     VEAGYQPELAYFEVLHEMKLIVDLMYEGGMENVRYSISNTAEFGDYVSGPRVITPDVK
                     ENMKAVLTDIQNGNFSNRFIEDNKNGFKEFYKLREEQHGHQIEKVGRELREMMPFIKS
                     KSIEK"
     gene            5204..6733
                     /locus_tag="NBI57_001299"
     CDS             5204..6733
                     /locus_tag="NBI57_001299"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HCD1822254.1"
                     /translation="MSSHIQIFDTTLRDGEQTPGVNFTFDERLRIALQLEKWGVDVIE
                     AGFPASSTGSFKSVQAIAQTLTTTAVCGLARCKKSDIDAVYEATKDAAKPVVHVFIAT
                     SPIHLEHKLKMSQEDVLASIKEHVTYAKQLFDVVQFSPEDATRTELPFLVKCVQTAVD
                     AGATVINIPDTVGYSYHDEYAHIFKTLTESVTSSNEIIYSAHCHDDLGMAVSNSLAAI
                     EGGARRIEGTVNGIGERAGNAALEEVALALYVRNDHYGAQTALNLEETKKTSDLISRY
                     AGIRVPRNKAIVGQNAFSHESGIHQDGVLKHRETYEIMTPQLVGVSTTELPLGKLSGK
                     HAFSEKLKALGYNIDKEAQIDLFKQFKTIADKKKSVSDRDIHAIIQGSEHEHQALYKL
                     ETLQLQYVSSGLQSAVVVVKDKEGHIYQDSSIGTGSIVAIYNAVDRIFQKETELIDYR
                     INSVTEGTDAQAEVHVNLLIEGKTVNGFGIDHDILQASCKAYVEAHAKFAAENVEKVG
                     N"
     gene            6736..7782
                     /gene="leuB"
                     /locus_tag="NBI57_001300"
     CDS             6736..7782
                     /gene="leuB"
                     /locus_tag="NBI57_001300"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HCD1822255.1"
                     /translation="MTYNIVALPGDGIGPEILNGSLSLLEIISNKYNFNYQIEHHEFG
                     GASIDTFGEPLTEKTLNACKRADAILLGAIGGPKWTDPNNRPEQGLLKLRKSLNLFAN
                     IRPTTVVKGASSLSPLKEERVEGTDLVIVRELTSGIYFGEPRHFNNHEALDSLTYTRE
                     EIERIVHVAFKLAASRRGKLTSVDKENVLASSKLWRKVVNEVSQLYPEVTVNHLLVDA
                     CSMHLITNPKQFDVIVCENLFGDILSDEASVIPGSLGLSPSASFSNDGPRLYEPIHGS
                     APDIAGKNVANPFGMILSLAMCLRESLNQPDAADELEQHIYNMIEHGQTTADLGGKLN
                     TTDIFEILSQKLNH"
     gene            7796..9166
                     /gene="leuC"
                     /locus_tag="NBI57_001301"
     CDS             7796..9166
                     /gene="leuC"
                     /locus_tag="NBI57_001301"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HCD1822256.1"
                     /translation="MGQTLFDKVWNRHVLYGKLGEPQLLYIDLHLIHEVTSPQAFEGL
                     RLQNRKLRRPDLTFATLDHNVPTIDIFNIKDEIANKQITTLQKNAIDFGVHIFDMGSD
                     EQGIVHMVGPETGLTQPGKTIVCGDSHTATHGAFGAIAFGIGTSEVEHVFATQTLWQT
                     KPKNLKIDINGTLPTGVYAKDIILHLIKTYGVDFGTGYALEFTGETIKNLSMDGRMTI
                     CNMAIEGGAKYGIIQPDDITFEYVKGRPFADNFAKSVDKWRELYSDDDAIFDRVIELD
                     VSTLEPQVTWGTNPEMGVNFSEPFPEINDINDQRAYDYMGLEPGQKAEDIDLGYVFLG
                     SCTNARLSDLIEASHIVKGNKVHPNITAIVVPGSRTVKKEAEKLGLDTIFKNAGFEWR
                     EPGCSMCLGMNPDQVPEGVHCASTSNRNFEGRQGKGARTHLVSPAMAAAAAIHGKFVD
                     VRKVVV"
     gene            9167..9739
                     /gene="leuD"
                     /locus_tag="NBI57_001302"
     CDS             9167..9739
                     /gene="leuD"
                     /locus_tag="NBI57_001302"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HCD1822257.1"
                     /translation="MAAIKPITTYKGKIVPLFNDNIDTDQIIPKVHLKRISKSGFGPF
                     AFDEWRYLPDGSDNPDFNPNKPQYKGASILITGDNFGCGSSREHAAWALKDYGFHIII
                     AGSFSDIFYMNCTKNAMLPIVLEKSAREHLAQYEEIEIDLPNQTVSSPDKRFHFEIDE
                     TWKNKLVNGLDDIAITLQYESLIEKYEKSL"
     gene            9754..11022
                     /gene="ilvA"
                     /locus_tag="NBI57_001303"
     CDS             9754..11022
                     /gene="ilvA"
                     /locus_tag="NBI57_001303"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase IlvA"
                     /protein_id="HCD1822258.1"
                     /translation="MTVKTTVSTKDIDEAFLRLKDIVKETPLQLDHYLSQKYDCKVYL
                     KREDLQWVRSFKLRGAYNAISVLSDEAKSKGITCASAGNHAQGVAYTAKKLNLNAVIF
                     MPVTTPLQKVNQVKFFGNSNVEVVLTGDTFDHCLAEALTYTSEHQMNFIDPFNNVHTI
                     SGQGTLAKEMLEQSKTDNVNFDYLFAAIGGGGLISGISTYFKTYSPTTKIIGVEPSGA
                     SSMYESVVVNNQVVTLPNIDKFVDGASVARVGDITFEIAKENVDDYVQVDEGAVCSTI
                     LDMYSKQAIVAEPAGALSVSALENYKDHIKGKTVVCVISGGNNDINRMKEIEERSLLY
                     EEMKHYFILNFPQRPGALREFVNDVLGPQDDITKFEYLKKSSQNTGTVIIGIQLKDHD
                     DLIQLKQRVNHFDPSNIYINENKMLYSLLI"
BASE COUNT         3894 a         1994 c         2245 g         3609 t
ORIGIN      
        1 gacaattcta actattaaag tgatatatac cattcacgga aggagtataa taaaatgctt
       61 aatcaatata ctgaacatca accgacaact tcaaatatta ttattttatt atactcttta
      121 ggactcgaac gttagtaaat atttactaaa cgctttaagt cctatttctg tttgaatggg
      181 acttgtaaac gtcccaataa tattgggacg tttttttatg ttttatcttt caattactta
      241 tttttattac tataaaacat gattaatcat taaaatttac gggggaattt actatgcgaa
      301 gcgacatgat caaaaaagga gatcaccaag caccagcaag aagtctttta catgccacgg
      361 gcgcgctaaa aagtccaact gatatgaaca aaccatttgt agctatttgt aactcttata
      421 ttgatattgt tcctggacat gttcacttga gagagcttgc agatatagct aaagaagcaa
      481 ttagagaagc cggtgccatt ccatttgaat tcaatacaat tggtgttgat gatggaatag
      541 ctatgggaca tatcggaatg cgatattctc taccatcacg tgaaattatt gcagatgcag
      601 ctgaaactgt aattaacgct cattggtttg acggcgtatt ttacattcct aattgtgaca
      661 agattacacc cggtatgatt ttagcagcca tgaggacaaa cgtaccagct atcttttgct
      721 ctggtggacc aatgaaagct ggcttatctg cacatggaaa agcattaaca ctttcatcaa
      781 tgtttgaagc agtcggcgca tttaaagaag gatcgatttc taaagaagaa tttttagata
      841 tggaacaaaa tgcctgccct acttgtggtt catgtgctgg gatgtttact gcaaattcaa
      901 tgaactgttt gatggaagtt ttaggtctag cattaccata caacggtact gcacttgcag
      961 tcagtgatca gcgacgcgaa atgattcgcc aagcagcttt taaattagtt gaaaatatta
     1021 aaaatgattt aaaaccacgt gatatcgtta ctcgcgaagc aattgatgat gcatttgcac
     1081 ttgatatggc tatgggtggt tcaacaaaca cggtactgca tacgttagcc attgccaatg
     1141 aagctggtat tgattatgac ttagagcgca ttaatgctat tgccaaacgc acgccatatt
     1201 tatcaaaaat agcacctagt tcatcgtatt caatgcatga tgtgcatgaa gctggtggcg
     1261 tcccagcaat tattaatgaa ttgatgaaga aagatggcac gttacaccca gatagaatca
     1321 cagttactgg caaaacgtta cgtgaaaata acgaaggcaa agaaattaag aactttgatg
     1381 tcattcaccc tcttgatgca ccatatgatg cacaaggcgg tttatctatc ttatttggta
     1441 atatcgcccc taaaggcgca gttattaaag ttggcggcgt tgatccatct atcaaaacat
     1501 ttactgggaa agcaatttgt ttcaattcgc atgatgaagc tgttgaagca atagacaatc
     1561 gtaccgttcg tgcaggccac gtcgttgtca ttagatatga aggacctaaa ggtggaccag
     1621 gtatgcctga aatgttagca cctacttcct ctattgttgg tcgcggctta ggtaaagatg
     1681 ttgcattaat tactgatggg cgtttttccg gtgccacaag aggtattgca gttggtcata
     1741 tttcccctga agctgcatct ggtggaccaa ttgccttaat tgaagatgga gatgagatta
     1801 ctattgattt aacaaatcgt acattaaacg taaaccagcc tgaagatgtt ctagcgcgtc
     1861 gccgagaatc tttaacaccg tttaaagcga aagtaaaaac aggttatcta gctcgttata
     1921 ctgccctagt aactagcgca aatacaggtg gcgtcatgca agtccctgag aatttaattt
     1981 aatttatttt tatattggag atggttaaaa tgtctaaaac tcaacatgaa gtaaaccaaa
     2041 atattgaccc tttaaaaatg gctgaatcac ttgaacttga acaactaaat gaaaaaactt
     2101 taaatgacat gcgttcagga tcagaagtgc tagtagaagc tctacttaaa gaaaatgtgg
     2161 attatttatt cggttatcct ggtggtgccg tactaccttt atacgacaca ttttatgatg
     2221 gtaaaatcaa acatatttta gcaagacacg aacaaggtgc tgttcatgct gcagaaggtt
     2281 atgcacgtgt atctggtaaa actggcgtcg ttgtagttac aagcggtcca ggtgcaacta
     2341 atgtaatgac aggtattacg gatgcacatt gcgactcttt acctctagtt gtattcactg
     2401 gacaagttgc tacaccaggc attggtaaag atgcattcca agaagcggat attctatcta
     2461 tgacttcacc aattacaaaa caaaattatc aagtgaaacg tgttgaagat atccctaaaa
     2521 tcgtacacga agctttccat gtagctaatt ctggacgcaa aggtcctgta gtgattgatt
     2581 ttccaaaaga tatgggtgtt ttagctacaa atgtggattt atgcgacgaa atcaatattc
     2641 caggttatga agttgttaca gaaccagaaa ataaagacat tgacactttc atctcacttt
     2701 taaaagaagc gaaaaagcct gtcgtattag ccggcgcagg tattaatcaa tcaaaatcaa
     2761 atcaattatt aacacagttt gttaataaac atcaaatccc aacagttact actttactag
     2821 gtctaggtgc tgtaccttat gaggatacac tatttttagg tatgggaggt atgcatggtt
     2881 cttatgctag taacatggca ttaactgagt gtgatttact cattaattta ggtagccgct
     2941 tcgatgatag attagcaagc aaacctgatg cctttgcacc taacgccaaa attgtacatg
     3001 tagatattga tccttcagaa atcaataaag ttattcatgt agatttaggt attattgcag
     3061 actgtaaaag atttttagaa tgtttaaatg ataaaaatgt tgagactata gaacacagtg
     3121 actgggttaa acattgtcaa aataataagc agaaacaccc atttaaactt ggtgaagaag
     3181 atcaagtatt ttgtaagcca caacaaacaa tcgaatatat cggcaaaatt acaaatggtg
     3241 aagcaattgt tactacagac gtgggacaac atcaaatgtg ggcagctcaa ttttatccat
     3301 ttaaaaatca cggacaatgg gttacaagcg gtggtttagg aacaatggga ttcggtattc
     3361 cttcgtcaat tggtgccaaa ttagctaatc ctgataaaac agtcgtatgt ttcgtcggtg
     3421 acggtggttt ccaaatgaca aaccaagaaa tggcactttt acccgaatat ggtttagatg
     3481 tcaaaatcgt actaatcaat aatggaacat taggtatggt taaacaatgg caagataagt
     3541 tctttaatca acgcttctca cactcagtat ttaatggtca acctgatttt atgaaaatgg
     3601 cagaagcata tggcgtcaaa ggtttcttaa tcgataagcc agaacaactg gaagaacaat
     3661 tagatgcagc gtttgcttat caaggaccag ttttaattga ggttcgtatt tcccctactg
     3721 aagctgtaac cccaatggtt ccgagtggca aatcaaatca tgaaatggag ggcttataat
     3781 gacaagaatt cttaaattac aagttgcgga tcaagtcagc acgctaaatc gaattacaag
     3841 tgcttttgtt cgcctacaat ataatatcga tacattacat gttacacatt ctgaacaacc
     3901 tgggatttct aacatggaaa ttcaagtcga tattcaagat gatacatcac ttcatatatt
     3961 aattaaaaaa ttaaaacaac aaattaatgt tttaacggtt gaatgctacg accttgttga
     4021 taacgaagct taattttaag acaaaggcaa tgatgcgcta attagttata gatatatcat
     4081 aggctgctag ttaacatctg ccactattac aaagttatat ttcagaattt tcgaaaaaca
     4141 aaacatttaa ttatttggag gaatttatta tgacaacagt ttattatgat caagacgtaa
     4201 aaacggacgc tttacaaggc aaaaaaattg cagtagtagg ttatggatca caaggccacg
     4261 cgcatgcaca aaacttaaaa gacaatggat atgatgtagt catcggtatt cgcccaggtc
     4321 gttcttttga caaggctaaa gaagatggat ttgatgtgtt ccctgttgca gaagcagtta
     4381 agcaagctga tgtaattatg gtgctattac ctgatgaaat tcaaggtgat gtatacaaaa
     4441 acgaaattga accaaattta gaaaaacata atgcgcttgc atttgctcat ggctttaaca
     4501 ttcattttgg tgttattcaa ccaccagctg atgttgatgt atttttagta gctcctaaag
     4561 gaccgggtca tttagttaga cgtacatttg ttgaaggttc tgctgtacca tcactatttg
     4621 gtattcaaca agacgcttca ggtcaagcac gtaatattgc tttaagttat gcaaaaggta
     4681 ttggtgcaac tcgtgcaggt gttattgaaa caacatttaa agaagaaact gagacagatt
     4741 tatttggtga acaagcagta ctttgcggtg gtgtatcgaa attaattcaa agtggctttg
     4801 aaacattagt agaagcgggt tatcaaccag aattagctta ttttgaagta ttacatgaaa
     4861 tgaaattaat cgttgatttg atgtatgaag gcggtatgga aaatgtacgt tactcaattt
     4921 caaatactgc tgaatttggt gactatgttt caggaccacg tgttatcaca ccagatgtta
     4981 aagaaaatat gaaagctgta ttaactgata tccaaaatgg taacttcagt aatcgcttta
     5041 tcgaagacaa taaaaatgga ttcaaagaat tttataaatt acgcgaagaa caacatggtc
     5101 atcaaattga aaaagttggt cgtgaattac gcgaaatgat gccttttatt aaatctaaaa
     5161 gcattgaaaa ataagataga cctacaatga ggagttgtta aatatgagta gtcatattca
     5221 aatttttgat acgacactaa gagacggtga acaaacacca ggagtgaatt ttacttttga
     5281 tgaacgcttg cgtattgcat tgcaattaga aaaatggggt gtagatgtta ttgaagctgg
     5341 atttcctgct tcaagtacag gtagctttaa atctgttcaa gcaattgcac aaacattaac
     5401 aacaacggct gtatgtggtt tagctagatg taaaaaatct gacatcgatg ctgtatatga
     5461 agcaacaaaa gatgcagcga agccggtcgt gcatgttttt atagcaacat cacctattca
     5521 tcttgaacat aaacttaaaa tgtctcaaga agacgtttta gcatctatta aagaacatgt
     5581 cacatacgcg aaacaattat ttgacgttgt tcaattttca cctgaagatg caacgcgtac
     5641 tgaattacca ttcttagtga aatgtgtaca aactgccgtt gacgctggag ctacagttat
     5701 taatattcct gatacagtcg gctacagtta ccatgatgaa tatgcacata ttttcaaaac
     5761 cttaacagaa tctgtaacat cttcaaatga aattatttat agtgctcatt gccatgacga
     5821 tttaggaatg gctgtttcaa atagtttagc tgcaattgaa ggcggtgcga gacgaattga
     5881 aggcactgta aatggtattg gtgaacgagc aggtaatgca gcacttgaag aagtcgcgct
     5941 tgcactatac gttcgaaatg atcattatgg tgctcaaact gcccttaatc tcgaagaaac
     6001 taaaaaaaca tcggatttaa tttcaagata tgcaggtatt cgagtgccta gaaataaagc
     6061 aattgttggc caaaatgcat ttagtcatga atcaggtatt caccaagatg gcgtattaaa
     6121 acatcgtgaa acatatgaaa ttatgacacc tcaacttgtt ggtgtaagca cgactgaact
     6181 tccattagga aaattatctg gtaaacacgc cttctcagag aagttaaaag cattaggtta
     6241 taacattgat aaagaagcgc aaatagattt atttaaacaa ttcaagacca ttgcggacaa
     6301 aaagaaatct gtttcagata gagatattca tgcgattatt caaggttctg agcatgagca
     6361 tcaagcactt tataaattgg aaacactaca actacaatat gtctctagcg gccttcaaag
     6421 tgctgttgtt gttgttaaag ataaagaggg tcatatttac caggattcaa gtattggtac
     6481 tggttcaatc gtagcaattt acaatgcagt tgatcgtatt ttccagaaag aaacagaatt
     6541 aattgattat cgtattaatt ctgtcactga aggtactgat gcccaagcag aagtacatgt
     6601 aaatttattg attgaaggta agactgtcaa tggctttggt attgatcatg atattttaca
     6661 agcctcttgt aaagcatacg tagaagcaca tgctaaattt gcagctgaaa atgttgagaa
     6721 ggtaggtaat taattatgac ttataacatt gttgccctac ctggtgatgg aatcggtcca
     6781 gaaattttga acggatctct atcattgctt gaaattataa gtaataaata taactttaat
     6841 tatcaaatag agcaccacga atttggtggt gcctctattg atacattcgg cgagccttta
     6901 actgagaaaa ccttaaatgc gtgtaaaaga gcagatgcta ttttactggg tgcaatcggt
     6961 ggacctaaat ggacagatcc taacaatcga ccagaacaag gattattaaa attgcgtaaa
     7021 tccttaaatt tatttgcaaa tattcgcccc actaccgttg tcaaaggcgc tagttcttta
     7081 tcacctttaa aggaagaacg cgttgaaggc acagatttag ttatagtccg tgaattgaca
     7141 agtggtattt attttggaga acctagacat tttaataatc acgaggcctt agattctctt
     7201 acttatacaa gagaagaaat agaacgcatt gttcacgtag catttaaatt ggccgcttca
     7261 agacgaggaa aactaacatc agttgataaa gaaaatgtat tagcttctag taaattgtgg
     7321 cgcaaagtcg taaatgaagt aagtcaatta tatccagaag taacagtaaa tcacttactt
     7381 gttgatgctt gtagtatgca tttaatcaca aatccaaaac aatttgacgt catcgtatgt
     7441 gaaaacttat ttggcgatat tttaagtgat gaagcttcag tgattcctgg ttcacttggt
     7501 ttatcacctt ctgctagttt tagtaacgat ggtccaagat tgtatgagcc tattcatgga
     7561 tcagcaccag atattgcagg taaaaacgtt gccaatccat ttggaatgat tctatcttta
     7621 gcgatgtgtt tacgtgaaag cttaaatcaa ccagatgctg cagatgaatt agaacaacat
     7681 atttataaca tgattgaaca tgggcaaacg acagcagatt taggcggcaa attgaatact
     7741 actgatattt tcgaaattct atctcaaaaa ttgaatcact aagggggaga tgtaaatggg
     7801 tcaaacatta tttgacaagg tgtggaacag acatgtgtta tacgggaaat tgggcgaacc
     7861 gcaactatta tacattgatt tacaccttat acatgaagtt acttctcctc aagcatttga
     7921 aggacttagg cttcaaaaca gaaaattaag acgcccagat ttaacatttg caacactcga
     7981 tcacaatgtt cctactattg atattttcaa tattaaagat gaaattgcaa acaaacaaat
     8041 cacaacatta caaaaaaacg ccatagattt tggggtgcat atttttgata tgggctctga
     8101 tgaacaaggt attgttcaca tggtaggacc tgagacagga cttacacagc ccggcaaaac
     8161 aatcgtttgt ggtgactctc acacagcaac acatggagca tttggtgcta ttgcatttgg
     8221 aattggaaca agtgaagttg aacatgtttt cgcaactcaa acgctatggc aaacaaaacc
     8281 caaaaactta aaaatcgata ttaatggtac cttaccaaca ggcgtctatg ctaaggacat
     8341 tattctgcat ttaattaaaa cgtatggtgt tgattttggt acaggctatg ctttggaatt
     8401 tactggcgaa acaattaaaa acctttcaat ggacggtcga atgactattt gtaacatggc
     8461 tatcgaaggt ggtgccaaat acggcataat ccaacctgat gatataacat ttgaatatgt
     8521 taaagggaga ccatttgccg ataacttcgc taaatcagtt gataagtggc gtgagctata
     8581 ttctgatgac gacgcgatat ttgatcgtgt aattgaactt gatgtttcaa cattagaacc
     8641 acaagtgaca tggggaacta atcctgaaat gggtgttaat ttcagtgaac cattccctga
     8701 aatcaatgat atcaacgatc aacgtgcgta tgattatatg gggttagaac caggtcaaaa
     8761 agctgaagac atcgacttag ggtatgtttt tctcggttca tgtacaaatg ctagactatc
     8821 agatttgatt gaagctagtc atattgttaa aggaaataaa gttcatccaa atattacagc
     8881 tattgtcgta ccaggttctc gtacagtaaa aaaagaagca gaaaaattag gtctagatac
     8941 tatctttaaa aatgcaggat ttgaatggcg tgaaccagga tgttcaatgt gtttaggcat
     9001 gaatcctgac caagtacctg agggcgtaca ttgtgcatct acaagtaatc gaaactttga
     9061 aggacgacaa ggcaaaggtg caagaacaca tttagtatcc cctgctatgg cagcagcagc
     9121 agctattcat ggtaaatttg tggacgtaag aaaggtggtt gtttaaatgg cagcaatcaa
     9181 acctattaca acatataaag gtaaaatagt ccctctcttc aacgacaata tcgatacaga
     9241 ccaaatcatt cctaaggtac acttaaagcg tatttcaaaa agtggctttg gtccatttgc
     9301 ttttgatgaa tggcggtact tacctgatgg ttcagataat cctgatttca atcctaacaa
     9361 accacaatat aaaggggctt ctattttaat tactggagat aattttggat gtggttcaag
     9421 tcgtgaacat gctgcttggg ctcttaagga ctatggtttt catattatta ttgcaggaag
     9481 tttcagtgac atattttata tgaattgcac taaaaatgcg atgttgccta tcgttttaga
     9541 aaaaagtgct cgtgaacatc ttgcacaata tgaggaaatt gaaatagatt taccaaatca
     9601 aactgtgtca tcaccagaca agcgtttcca ttttgaaatt gatgaaactt ggaaaaataa
     9661 acttgtaaat ggcttagatg acattgcaat caccctacaa tatgaatcat taatagaaaa
     9721 atatgaaaaa tcactttaag ggagttgaat attatgacag tcaaaacaac agtttctacg
     9781 aaagatatcg atgaagcatt tttaagactt aaagatattg tcaaagaaac acctttacaa
     9841 ttagaccatt acttatctca aaagtatgat tgtaaagttt atttaaaacg agaagattta
     9901 caatgggtac gttcttttaa attaagaggt gcttacaacg ctatttctgt tttatcagat
     9961 gaagctaaaa gtaaaggtat tacatgtgcg agtgcaggta atcatgctca aggtgttgcc
    10021 tatacagcta aaaaacttaa tttaaacgct gttatcttta tgccagtcac tacaccttta
    10081 caaaaggtaa atcaagtaaa gttctttgga aatagtaacg ttgaagttgt actcactggt
    10141 gatacatttg atcactgttt agctgaagct ttaacttata caagtgaaca tcaaatgaac
    10201 tttatagatc cattcaataa tgttcataca atttctggac aaggtacgct tgctaaagaa
    10261 atgctagaac aatcaaagac tgacaatgtt aactttgatt atttatttgc cgctattggt
    10321 ggtggtggat taatttcagg tattagtact tactttaaaa cctattcacc tactacgaaa
    10381 attataggtg ttgaaccttc aggtgcaagt agtatgtatg aatctgttgt cgtaaataat
    10441 caggtagtca cattgcctaa tatcgataaa tttgtggacg gtgcatctgt agctagagtt
    10501 ggcgatatta catttgaaat tgcaaaagaa aatgtagatg attacgttca agtagatgaa
    10561 ggtgcagttt gttctacgat tttagacatg tattcaaaac aagcaattgt agcagaacct
    10621 gctggcgcat taagtgtgag tgctcttgaa aactacaaag accacattaa gggtaaaact
    10681 gttgtttgtg tcattagtgg tggtaataat gatattaatc gtatgaagga aattgaagaa
    10741 cgttctttat tatatgaaga aatgaaacat tattttattt taaacttccc tcaacgccct
    10801 ggtgcattaa gagaatttgt aaatgatgtt ttaggcccac aagacgatat tacaaaattt
    10861 gaatacttaa aaaaatcttc tcaaaataca ggtactgtca ttattggtat tcaacttaaa
    10921 gatcatgatg atttaataca actcaaacaa cgtgtaaatc atttcgatcc ttccaatatt
    10981 tatattaatg aaaataagat gttatattca ttgttaattt aacacatagt aagaaaaaca
    11041 gtcataaatt gatttctaat tgaaatcatc ttatgactgc tttttattat actttacatt
    11101 tctcgtttcg tcagattcaa acgttttcac ttcgccaagc catctttctt tgtgtttgct
    11161 ttttactatg taactttaga cataaaaaaa gagaccttgc ggtctcaatg cggctcatcg
    11221 catccacttt ttgcctggca acgttctact ctagcggaac gtaagttggc taccttcgtc
    11281 gctaagaacc tttcttgact tgtgacaatc gcttgcttct ttcctctcct tcggctctcg
    11341 cttactcatt tagctctact aaactcgttg cactcttttc tcgtttcgtc agattcaaac
    11401 gttttcactt cgccaagcca tctttctttg tgtttgcttt tattttgacg ttttagacat
    11461 aaaaaaagag accttgcggt ctcaacttgc ctggcaacgt tctactctag cggaacgtaa
    11521 gtttgctacc atcgtcgcta aagacctttc ttgacttgtg acaatcgctt gcttctttcc
    11581 tctccttcgg ctctcgctta ctcatttagc tctactaaac tcgttgcgct cttttcttgt
    11641 ttcgtcagat tcaaacgttt tcacttcgcc aagccatctt tctttgtgtt tgcttttatt
    11701 ttgacgtttt agacataaaa aaaagagacc tgcggtctca ac
//
LOCUS       DAHHSC010000063        11652 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.063, whole genome shotgun sequence.
ACCESSION   DAHHSC010000063 DAHHSC010000000
VERSION     DAHHSC010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 11652)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11652)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11652
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.063"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            125..1948
                     /gene="lepA"
                     /locus_tag="NBI57_001304"
     CDS             125..1948
                     /gene="lepA"
                     /locus_tag="NBI57_001304"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HCD1822259.1"
                     /translation="MDNEQRLKRRENIRNFSIIAHIDHGKSTLADRILENTKSVETRD
                     MQDQLLDSMDLERERGITIKLNAVRLKYEAKDGNTYTFHLIDTPGHVDFTYEVSRSLA
                     ACEGAILVVDAAQGIEAQTLANVYLALDNELELLPVINKIDLPAAEPERVKQEIEDMI
                     GLDQDDVVLASAKSNIGIEEILEKIVEVVPAPDGDPEAPLKALIFDSEYDPYRGVISS
                     IRIVDGVVKAGDKIRMMATGKEFEVTEVGINTPKQLPVDELTVGDVGYIIASIKNVDD
                     SRVGDTITLASRPASEPLQGYKKMNPMVYCGLFPIDNKNYNDLREALEKLQLNDASLE
                     FEPESSQALGFGYRTGFLGMLHMEIIQERIEREFGIELIATAPSVIYQCILRDGSEVT
                     VDNPAQMPDRDKIDKIFEPYVRATMMVPNDYVGAVMELCQRKRGQFINMDYLDDIRVN
                     IVYELPLAEVVFDFFDQLKSNTKGYASFDYEFIENKESNLVKMDILLNGDKVDALSFI
                     VHRDFAYERGKALVEKLKTLIPRQQFEVPVQAAIGQKIVARTNIKSMGKNVLAKCYGG
                     DISRKRKLLEKQKAGKAKMKAVGNVEIPQDAFLAVLKMDDE"
     gene            2455..3579
                     /locus_tag="NBI57_001305"
     CDS             2455..3579
                     /locus_tag="NBI57_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HCD1822260.1"
                     /translation="MTVQSAYIHIPFCVRICTYCDFNKYFIQNQPVDEYLDALITEMS
                     TAKYRNLKTMYVGGGTPTALSINQLERLLKAIRDTFTITGEYTFEANPDELTKEKVQL
                     LEKYGVNRISMGVQTFKPELLSVLGRTHNTEDIYTSVLNAKNAGIKSISLDLMYHLPK
                     QTIEDFEQSLDLALDMDIQHISSYGLILEPKTQFYNMYRKGLLKLPNEDLGADMYQLL
                     MSKIEQSPFHQYEISNFALDGHESEHNKVYWFNEEYYGFGAGASGYVDGVRYTNINPV
                     NHYIKAINKESKAILVSNKPSLTERMEEEMFLGLRLNEGVSSSRFKKKFDQSIESVFG
                     QTINNLKEKELIVEKNDAIALTKRGKVIGNEVFEAFLIND"
     gene            3680..4657
                     /gene="hrcA"
                     /locus_tag="NBI57_001306"
     CDS             3680..4657
                     /gene="hrcA"
                     /locus_tag="NBI57_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcription repressor HrcA"
                     /protein_id="HCD1822261.1"
                     /translation="MITDRQLSILNAIVEDYVDFGQPVGSKTLIERHNLNVSPATIRN
                     EMKQLEDLNYIEKTHSSSGRSPSQLGFRYYVNRLLEQTSHQKTNKLRRLNQLLVENQY
                     DVSSALTYFADELSNISQYTTLVVHPNHKQDIINNVHLIRANPNLVIMVIVFSSGHVE
                     HVHLASDIPFSNDKLNTISNFVTNKLTEFNQNLQDDIVSFVQSEQEEIFINKLINTMN
                     NHISNQSNSIYMGGKVKLIDALNESNVSSIQPILQYIESNRIAELLQDISSPNINVKI
                     GNEIDDSLSDISIVTSQYHFDETLKGQIAVIGPTAMHYQNVIQLLNRIW"
     gene            4689..5315
                     /gene="grpE"
                     /locus_tag="NBI57_001307"
     CDS             4689..5315
                     /gene="grpE"
                     /locus_tag="NBI57_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HCD1822262.1"
                     /translation="MTNKDESVEKNTESTVEETNIKQNIDDSVEQAEESKGHLQDEAI
                     EETSDENVIEEIDPKDQKINELQQLADENEEKYLRLYAEFENYKRRIQKENEINKTYQ
                     AQRVLTDILPAIDNIERALQIEGDDETFKSLQKGVQMVHESLINALKDNGLEVIKTEG
                     EAFDPNIHQAVVQDDNPDFESGEITQELQKGYKLKDRVLRPSMVKVNQ"
     gene            5384..7216
                     /gene="dnaK"
                     /locus_tag="NBI57_001308"
     CDS             5384..7216
                     /gene="dnaK"
                     /locus_tag="NBI57_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HCD1822263.1"
                     /translation="MSKIIGIDLGTTNSCVTVLEGDEPKVIQNPEGSRTTPSVVAFKN
                     GETQVGEVAKRQAITNPNTVQSIKRHMGTDYKVDIEGKSYTPQEISAMILQNLKNTAE
                     SYLGEKVDKAVITVPAYFNDAERQATKDAGKIAGLEVERIINEPTAAALAYGLDKTDK
                     DEKVLVFDLGGGTFDVSILELGDGVFEVLSTAGDNKLGGDDFDQVIIDYLVAEFKKEN
                     GVDLSQDKMALQRLKDAAEKAKKDLSGVSQTQISLPFISAGENGPLHLEVNLTRSKFE
                     ELSDSLIRRTMEPTRQAMKDAGLTNSDIDEVILVGGSTRIPAVQEAVKKEIGKEPNKG
                     VNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIEILGGRMNTLIERNTTIPTSK
                     SQIYSTAVDNQPSVDVHVLQGERPMAADNKTLGRFQLTDIPPAERGKPQIEVTFDIDK
                     NGIVNVTAKDLGTNKEQRITIQSSSSLSDEEIDRMVKDAEVNAEADKKRREEVDLRNE
                     ADSLVFQVEKTLTDLGENIGEEDKKSAEEKKDALKTALEGQDIEDIKSKKEELEKVIQ
                     ELSAKVYEQAAQQQQQAQGANAGQNNDSTVEDAEFKEVKDDDKK"
     gene            7352..8491
                     /gene="dnaJ"
                     /locus_tag="NBI57_001309"
     CDS             7352..8491
                     /gene="dnaJ"
                     /locus_tag="NBI57_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HCD1822264.1"
                     /translation="MAKRDYYEVLGISKDASKDEIKKAYRKLSKKYHPDINKEEGADE
                     KFKEISEAYEVLSDDNKRASYDQFGHDGPQGFGGQGFNGSDFGGFSGFGGGGFEDIFS
                     SFFGGGRQRDPNAPQKGDDLQYTMTLTFEEAVFGTTKEISIRKDVTCETCHGDGAKPG
                     TSKKTCSYCNGAGHVAVEQNTILGRVRTEQVCPKCNGSGQEFEEACPTCHGKGTENKT
                     VKLEVKVPEGVDNEQQIRLAGEGSPGVNGGPAGDLYVVFRVKPSETFKRDGDDIYYKL
                     NVSFPQAALGDEIKIPTLNNEVMLTIPAGTQTGKQFRLKEKGIKNVHGYGYGDLYVDI
                     KVVTPTKLTDRQKELMKEFAQLNGEEINEQPSNFKDRAKRFFKGE"
     gene            8495..9433
                     /gene="prmA"
                     /locus_tag="NBI57_001310"
     CDS             8495..9433
                     /gene="prmA"
                     /locus_tag="NBI57_001310"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HCD1822265.1"
                     /translation="MNWTELSIIINHEAVELATNILENHGSNGVVIEDSDDLINQPED
                     KYGEIYALKKEDYPDKGVRLKAYFNEMTYDDKLRQQIKDELLNLDELDQHNIQFSEQI
                     IAETDWENEWKNYFHPFRASKKFTIVPSWETYAKEADEELCIELDPGMAFGTGDHPTT
                     SMCLKAIETYVLPQHSVIDVGTGSGILSIASHLIGVKRIKALDIDEMAVSVAKENFRR
                     NHCETLIEAVPGNLLKDETEKFDIVIANILAHIIDEMIEDAYNTLNEGGYFITSGIIK
                     EKYEGIQSHMERVGFKIISEQHDNGWVCLVGQKVSE"
     gene            9435..10187
                     /locus_tag="NBI57_001311"
     CDS             9435..10187
                     /locus_tag="NBI57_001311"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HCD1822266.1"
                     /translation="MQRYFIDQNADVSQRFFITKKEDIHHITNVMRYDVGSKIILTFN
                     DQTVYLCEIADITSDKIAVDLKEKQNINTELPVDVTICSGLIKADKYEWLLQKATELG
                     ASSFIAVSMERSIVKLNEAKVVKKIERWQKIIKEAAEQSYRLVIPSIQFESNLKLICD
                     TIDNYDYILIAYEEEAKDGELSNFKQTLQQFKAQDKVLMIFGPEGGLSENEISLFSNT
                     STVVGLGPRILRAETAPLYALSAISYEKELMG"
     gene            10194..11540
                     /gene="mtaB"
                     /locus_tag="NBI57_001312"
     CDS             10194..11540
                     /gene="mtaB"
                     /locus_tag="NBI57_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase MtaB"
                     /protein_id="HCD1822267.1"
                     /translation="MSTVAFHTLGCKVNHYETEAIWQLFKEANYERVDFEANADVFVI
                     NTCTVTNTGDKKSRQIIRRAIRQNPDAVICVTGCYAQTSSAEIMEIPGVDVVVGTQDR
                     HKLLGYIDEFRKERQPINGVGNIMKNRKYEELDVPYFTDRTRASLKIQEGCNNFCTFC
                     IIPWARGLMRSRDPEKVVEQATQLVNSGYKEIVLTGIHTGGYGQDLKDYNLAQLLRDL
                     ETINGLERIRISSIEASQLTDEVIDVLERSTKVVRHLHIPLQSGSDTVLKRMRRKYTM
                     DRFSERLTKLHKALPDLAVTSDVIVGFPGETEAEFQETYDFIVKHKFSELHVFPYSPR
                     IGTPAARMDDQIDEEIKNERVHKLITLSNQLGKLYASKFDQDVLEVIPEEQGDTEGTL
                     VGYADNYMKVQFEGDESLIGQIVKVKITQANYPLNEGQAIKVVDFATNKSDREVLV"
BASE COUNT         4394 a         1592 c         2270 g         3396 t
ORIGIN      
        1 aagattgtta taatttcaaa gttcatccaa gattatggcg tttgcattta cctattaaaa
       61 acgttattat atcaaagatg cgaaagataa tacgggttta ttttatgaaa gtgagaagga
      121 taaaatggat aatgagcaac gcttaaaaag aagagagaat ataaggaatt tctcgattat
      181 agcacatatt gaccacggaa aatctacatt ggctgataga attttagaaa ataccaaatc
      241 agttgaaaca agagatatgc aagatcagtt actagattca atggatttag aaagagaacg
      301 tggtattaca atcaaattaa acgcagttcg tttaaagtac gaagctaaag atggaaatac
      361 ttatacattc catttaatcg atacgcctgg acacgtcgat tttacatatg aagtgtcacg
      421 ttctttggca gcttgtgagg gtgcgatttt agtagtagat gcggctcaag gtatcgaagc
      481 acaaacatta gcaaatgttt atttggcatt agataatgag ttagagttat tgcctgttat
      541 taacaaaatt gatttacctg ctgcagaacc tgaacgcgtg aaacaagaaa ttgaagatat
      601 gataggttta gaccaagacg atgttgtttt agcaagtgct aaatctaaca ttggaattga
      661 agagatacta gagaaaatag ttgaagttgt gccagctcca gatggtgacc cagaagcacc
      721 actaaaagca ttaatatttg attctgagta tgatccatat agaggggtaa tttcatcgat
      781 aagaattgta gacggtgttg ttaaagctgg agataaaatt cgaatgatgg ccactggtaa
      841 agagttcgaa gtaacagaag ttggaattaa tacacctaag cagcttccag ttgatgaatt
      901 aacagttggt gatgttggtt atattattgc aagtattaaa aatgttgatg attctagggt
      961 tggtgacacc atcacattag ctagtagacc tgcatcagaa ccattgcaag gttataagaa
     1021 aatgaatcca atggtatatt gcggactgtt cccaatagat aacaaaaatt ataatgattt
     1081 aagagaagca ttagaaaaat tacaattgaa tgatgcatca ttagaatttg agcctgaatc
     1141 gtcacaagca ttaggttttg gttatagaac tggtttctta ggtatgttac acatggaaat
     1201 aattcaagaa agaattgaaa gagaatttgg cattgaatta attgcaacag caccatctgt
     1261 aatttatcaa tgtattttaa gggacggttc agaagtgacg gttgataacc cagcacaaat
     1321 gccagatcgt gataaaattg ataaaatatt tgagccatat gttcgtgcaa ctatgatggt
     1381 tccaaatgac tatgtaggtg cagtaatgga attatgtcaa cgtaaacgtg gacaatttat
     1441 aaatatggac tatttagatg atattcgtgt aaatattgtt tatgaattac ctttagctga
     1501 agttgtattt gatttcttcg atcaacttaa atctaatact aaaggatatg catcatttga
     1561 ttatgaattc atcgaaaata aagaaagtaa tttagttaag atggatattt tattaaacgg
     1621 tgataaagtg gatgcgctaa gcttcatagt tcatagagat tttgcatatg aacgtggtaa
     1681 agcattagtt gaaaaactta aaacgttaat tccaagacag caatttgaag tacctgtaca
     1741 ggctgcaata ggacaaaaaa ttgtagcgcg tacaaatatt aaatcaatgg gtaaaaacgt
     1801 tttagctaaa tgttatggcg gtgacataag ccgtaaacgt aaattacttg aaaaacaaaa
     1861 agcaggtaaa gctaagatga aagcagttgg taatgttgaa attccacagg atgctttctt
     1921 ggctgtattg aaaatggatg acgaataagt ttttaaaaaa tttattaaca atttacaatg
     1981 aataaagttt aataactaaa aagagggagc ctaggataaa ttaacgtcct gggctttaca
     2041 atgttatatt ggcagtcatc gacagagtta aaatgagctt ataacaatgg ggccccaaca
     2101 cagagaattt cgaaaagaaa ttctacaggc aatgcaagtt ggggtgggac aacgaaataa
     2161 attttgcgaa aatatcattt ctgtcccact cccttatgca tgagttttac tcatgtaatt
     2221 ttatttttaa ggacatatta catctggcta atgtgtaaga gccactacat aataaatcat
     2281 tagtggttct ttattatttc tatctcactc cctctaaaca agaataaata tcaaaatgaa
     2341 tcgatatatt agacaaccat tgattaaacg ttaaagttaa aagtaagaat aacagcagat
     2401 agtccaacag gatgtagccg attggataaa cagtctgaga agcggggcat taaaatgacg
     2461 gtacaaagtg catatataca tattccattt tgtgtaagaa tatgtacata ttgtgatttc
     2521 aataaatatt ttatacagaa tcaacctgta gatgagtact tagatgcact aataacagaa
     2581 atgtctacag caaaatatag gaacttaaag accatgtatg taggtggcgg cacaccaacg
     2641 gccctttcta ttaatcagtt ggaaagatta cttaaagcaa tacgtgatac gtttacaatc
     2701 acaggcgagt atacatttga agcaaatcct gatgagttaa ctaaagagaa agtccaacta
     2761 ttagagaaat atggagtaaa tagaatttca atgggcgttc aaacattcaa accggagtta
     2821 ttgtctgttt taggtagaac gcacaatact gaagatattt acacttcggt gttaaatgct
     2881 aaaaacgcag gtattaaatc aatcagttta gatttaatgt atcatttacc gaaacagacg
     2941 attgaagatt ttgaacaaag tttagatcta gctttagata tggatattca acatatttcg
     3001 agttacggct taatacttga acctaaaacc caattttata atatgtatag aaaagggttg
     3061 cttaaacttc ctaatgagga tttaggtgct gacatgtatc agttgttgat gtctaagata
     3121 gaacaatctc ctttccatca atacgaaata tctaattttg cattagatgg ccatgaatca
     3181 gaacataata aggtttactg gtttaatgag gaatattatg gatttggagc aggtgcaagt
     3241 ggttatgtag atggtgtgcg ttatacgaat atcaatccag tgaatcatta tatcaaagct
     3301 ataaataaag aaagtaaagc aattttagta tcaaataaac cttctttgac tgagagaatg
     3361 gaagaagaaa tgtttcttgg gttgcgttta aatgaaggtg tgagtagtag taggttcaaa
     3421 aagaagtttg accaatctat tgaaagtgtc tttggtcaaa caataaataa tttaaaagag
     3481 aaagaattaa ttgtagaaaa gaacgatgcg attgcactaa caaagcgagg gaaagtcata
     3541 ggtaatgagg tttttgaagc tttcctaata aatgattaaa aaaaattgaa atttcgagtc
     3601 tttaacattg acttactttg accaatttga taaattataa ttagcacttg agataagtga
     3661 gtgctaatga ggtgaaaaca tgattacaga taggcaattg agtatattaa acgcaattgt
     3721 tgaggattat gttgattttg gacaacccgt tggttctaaa acactaattg agcgacataa
     3781 cttgaatgtt agtcctgcta caattagaaa tgagatgaaa cagcttgaag atttaaacta
     3841 tatcgaaaag acacatagtt cttcagggcg ttcgccatca caattaggtt ttaggtatta
     3901 tgtcaatcgt ttacttgaac aaacatctca tcaaaaaaca aataaattaa gacgattaaa
     3961 tcaattgtta gttgagaatc aatatgatgt atcatcagca ttgacatatt ttgcagatga
     4021 attatcaaat atatctcaat atacaacttt agttgttcat cctaatcata aacaagatat
     4081 tatcaataat gtacatttga ttcgtgctaa tcctaattta gttataatgg ttatcgtatt
     4141 ttcatcaggt catgttgagc atgtacatct tgcttcagac ataccgttta gtaatgataa
     4201 actaaataca atatcaaatt ttgttactaa caaactaact gagtttaatc agaatttaca
     4261 agatgatatt gtctcttttg ttcaatcaga gcaggaagaa atatttatca ataaattgat
     4321 aaatacgatg aataatcata tttctaatca aagtaatagc atttatatgg gtggaaaggt
     4381 taaacttatt gatgcattga atgaaagtaa tgtatcttca attcagccca ttctacaata
     4441 tatagaatca aatagaattg cagaattatt acaagatata tcttcaccaa atattaatgt
     4501 aaaaattggt aatgaaattg atgacagctt aagtgatatt tcaattgtaa caagtcaata
     4561 tcattttgat gaaacgttaa aaggtcaaat cgctgtgatt ggacctacag ctatgcatta
     4621 tcaaaatgtc attcaattat taaatagaat ttggtaatga tggattagaa agtattggag
     4681 gacagaaaat gacaaataaa gacgaatcag ttgaaaaaaa cactgaatca acagttgaag
     4741 aaacaaacat caaacaaaat attgatgatt cagttgaaca agctgaagaa agtaaaggtc
     4801 atttacaaga tgaagcaata gaagaaacgt ctgacgagaa tgttattgaa gaaatagatc
     4861 caaaagatca aaaaattaat gaacttcaac aattagcaga tgaaaacgaa gagaaatatt
     4921 taaggctcta cgctgagttt gaaaattata agcgtagaat tcaaaaagaa aatgaaataa
     4981 ataaaacata tcaagcacaa cgtgtgttaa cagatatttt accagcaata gacaatatag
     5041 aacgtgcact tcaaattgaa ggtgatgatg agacttttaa atctcttcaa aaaggtgtac
     5101 aaatggtgca tgaaagtttg attaacgcac taaaagataa tggtcttgaa gttattaaaa
     5161 ctgaaggtga agcatttgat ccaaatattc accaagctgt agttcaagat gataaccctg
     5221 attttgaatc tggcgaaatc actcaagaac tacaaaaagg atacaagctt aaagatagag
     5281 tattaagacc atcaatggtc aaagtaaacc aataacttaa atttggcgaa aagacattgt
     5341 ttaaaattaa attaatttaa tgattaaatg gaggaatttt attatgagta aaattattgg
     5401 tatagactta ggtacaacaa attcatgtgt aacagtatta gaaggcgatg agccaaaagt
     5461 aattcaaaac cctgaaggtt cacgtacaac accatctgtt gtagctttca aaaatggaga
     5521 aactcaagtt ggtgaagtag ctaaacgtca agctattaca aacccaaaca ctgttcaatc
     5581 tattaaacgt catatgggta ctgattataa agtagatatt gaaggtaaat catacacacc
     5641 acaagaaatc tcagctatga ttttacaaaa cttaaaaaat acagctgaaa gctatttagg
     5701 tgagaaagtt gacaaagctg taattacagt acctgcatac tttaacgacg ctgaacgtca
     5761 agcaactaaa gatgctggta aaattgctgg tttagaagtt gagcgtatca ttaatgaacc
     5821 aacagctgca gcattagcat atggtttaga taaaactgat aaagatgaaa aagttcttgt
     5881 ttttgactta ggtggcggta catttgacgt atctatccta gaattaggtg acggtgtatt
     5941 cgaagtacta tcaacagccg gtgacaacaa acttggcggt gatgattttg accaagtaat
     6001 tattgactac ctagttgcag aattcaaaaa agaaaatggc gtagacttat ctcaagataa
     6061 aatggcatta caacgtttga aagatgctgc tgaaaaagct aaaaaagact tatcaggtgt
     6121 atcacaaact caaatctcat taccatttat ctcagctggt gaaaacggtc cattacactt
     6181 agaagtaaac ttaactcgtt ctaaatttga agaattatca gattcattaa ttagaagaac
     6241 aatggaacct acacgccaag caatgaaaga cgctggctta acaaactcag atatcgatga
     6301 agttatctta gttggtggat caactcgtat tccagcagta caagaagctg tcaaaaaaga
     6361 aatcggtaaa gaacctaaca aaggtgtaaa cccggacgaa gtagtggcaa tgggagctgc
     6421 aatccaaggt ggcgttatca caggtgacgt taaagacgta gtattattag acgtaacacc
     6481 actatcttta ggtattgaaa ttttaggtgg acgtatgaat acgttaattg aacgtaacac
     6541 tacgattcct acatctaaat ctcaaatcta ttcaacagca gtagataatc aaccatcagt
     6601 agatgtacac gtattacaag gtgaacgtcc aatggctgcg gataataaaa cacttggtag
     6661 attccaattg actgatattc caccagctga acgtggtaaa cctcaaattg aagtaacgtt
     6721 tgatatcgat aaaaacggta ttgtaaatgt aactgcaaaa gacttaggta caaataaaga
     6781 acaaagaatt acaattcaat caagttcttc attatcagac gaagaaatcg accgtatggt
     6841 aaaagatgct gaagttaacg ctgaagcaga taaaaaacgt cgtgaagaag tagacttaag
     6901 aaacgaagct gacagtctag tattccaagt tgaaaaaact ttaactgatt taggcgaaaa
     6961 tattggtgaa gaagataaaa aatctgctga agagaaaaaa gacgctctta aaactgcttt
     7021 agaaggtcaa gatatagaag atattaaatc taaaaaagaa gaacttgaaa aagtgattca
     7081 agaattatca gcaaaagtat atgagcaagc ggctcaacag caacaacaag cacaaggtgc
     7141 aaatgctggt caaaacaacg atagtactgt agaagatgct gaatttaaag aagtaaaaga
     7201 cgacgacaaa aaataattaa acaatacaat tagaatgttg tcatgatctg aaaagtcaaa
     7261 gtcaatagaa cattggcttt ggctttttat ttggatagta tgacatatta cgttaaacta
     7321 tatgagttaa ttaagaaaag gagagatagc tgtggccaaa agagactatt atgaagtttt
     7381 aggcattagt aaagatgctt caaaagatga aatcaaaaaa gcgtatcgaa agctttcgaa
     7441 aaaatatcat ccagatatta acaaagaaga aggtgcagat gaaaagttta aagaaatttc
     7501 tgaagcctat gaagttttaa gtgatgataa taaacgcgca agctatgatc aatttggaca
     7561 tgatggtcca caaggttttg gtggacaagg attcaatggc tctgactttg gcggttttag
     7621 tggcttcggt ggcggcggtt ttgaagatat ctttagctct ttcttcggtg gcggcagaca
     7681 aagagatcca aatgcaccgc aaaaaggtga tgatcttcag tatacaatga cattaacatt
     7741 tgaagaagcg gtatttggta caacaaaaga aatttcaatc cgtaaagatg taacatgcga
     7801 aacatgtcat ggtgatggtg caaagcctgg aacaagtaaa aagacttgta gttactgtaa
     7861 tggcgctggt catgtagctg tagaacaaaa cacaatttta ggtagagttc gtactgaaca
     7921 agtttgtcct aaatgtaatg gaagtggtca agaatttgaa gaagcttgtc caacatgtca
     7981 cggaaaaggt actgaaaata aaacagttaa attagaagtg aaagtacctg aaggtgtcga
     8041 caatgaacaa caaattagat tagctggtga aggctctcca ggcgtgaatg gaggacctgc
     8101 tggtgattta tacgtagtat ttagagttaa accatctgaa acatttaaac gtgatggtga
     8161 tgatatttat tacaaattaa atgttagctt cccacaagca gctttaggcg atgaaataaa
     8221 aataccaaca ttaaataatg aagttatgtt aacaatccct gctggtacac aaacaggtaa
     8281 gcaattccgc ttaaaagaaa agggtataaa aaatgttcat ggctatggat acggagattt
     8341 atatgttgat attaaagtag taacgcctac taaattaaca gatagacaaa aagaactaat
     8401 gaaagaattt gcccaattaa atggtgaaga aattaatgaa caaccttcga attttaaaga
     8461 tagagcaaaa agattcttta agggagaata aagtatgaac tggacagagc tttcaattat
     8521 tattaatcat gaagcagtag aattggctac caatatactt gaaaatcatg gatcaaatgg
     8581 tgtcgtgata gaagattcag atgatttaat taaccaacca gaagataaat acggtgaaat
     8641 ttacgctttg aaaaaagagg attatccaga taagggagta agattgaaag cctattttaa
     8701 tgaaatgact tatgatgata agttgcgaca gcaaattaaa gatgagttat taaatttaga
     8761 tgaacttgat caacataaca ttcaattcag tgagcaaatt attgcagaga cggattggga
     8821 aaatgaatgg aaaaactatt tccatccatt ccgagcgtcg aagaagttca caatagttcc
     8881 tagttgggaa acatatgcta aagaagcgga tgaagagctt tgcattgagc tcgacccagg
     8941 tatggctttt ggaacaggcg atcatccgac tacaagtatg tgtttgaagg caatagaaac
     9001 atatgtattg ccacagcatt cagtaattga tgttggtact ggctcaggta tattaagtat
     9061 tgcaagtcat ctaatcggtg taaaacgtat taaagcgttg gatattgatg aaatggcagt
     9121 gagtgtagct aaagaaaact tcagaagaaa tcattgtgaa acgttaattg aagctgttcc
     9181 aggtaactta ttgaaagacg aaacagaaaa atttgatatt gtaatagcaa atattttagc
     9241 gcatattatt gatgaaatga ttgaagatgc ttataatact ctaaatgaag gcggttattt
     9301 tattacttct ggtattataa aagagaagta tgaaggtata cagtcacata tggagcgtgt
     9361 aggttttaaa attatttcag aacaacatga caatggttgg gtttgtcttg ttggccagaa
     9421 agtgagtgaa taatgtgcaa cgttatttca tagaccaaaa cgctgatgta agtcagcgtt
     9481 tttttattac aaaaaaagaa gatattcatc atataacaaa tgtaatgaga tatgatgttg
     9541 gtagtaaaat tattttaact tttaatgatc aaacagttta tttatgtgaa attgctgaca
     9601 taacaagtga taaaattgca gttgatttaa aagaaaaaca aaatattaat acagaattgc
     9661 cagttgatgt tacgatttgc agtggactaa ttaaagctga caaatatgag tggttgctac
     9721 aaaaagctac tgaattgggt gcttcatcat ttatagctgt gagcatggaa cgctcaattg
     9781 ttaaattaaa cgaggcgaaa gttgtcaaaa agatagagcg ttggcaaaaa ataattaaag
     9841 aagctgctga acaaagctat cgtttagtga ttccatctat tcaattcgag tcgaatttaa
     9901 aattaatttg tgatactatt gataattatg actatattct tattgcatat gaagaggaag
     9961 caaaagatgg tgagttaagc aatttcaagc aaactttaca acaattcaag gctcaggata
    10021 aagtgttgat gatttttggt cctgagggtg gtttgtcaga aaatgaaatt tcattattta
    10081 gtaatactag tacagttgtt ggtcttggac cgagaatttt acgggcagaa actgcgccat
    10141 tgtatgcgtt aagtgcaata agttatgaaa aagaattaat ggggtgaata tttatgtcaa
    10201 cagttgcgtt tcacacatta ggttgtaaag taaaccatta tgaaactgaa gcaatctggc
    10261 aattatttaa agaagcaaac tatgagcgcg ttgactttga agcgaatgct gatgtatttg
    10321 ttattaatac ttgtacagta acgaatacag gtgataaaaa aagtcgtcaa ataattagac
    10381 gtgcaataag acaaaatcct gatgctgtaa tctgtgtaac aggttgttat gcgcaaactt
    10441 catcagctga aattatggaa attcctggtg tcgatgtagt agttggtaca caagatagac
    10501 ataaactatt aggttacatt gatgaattcc gtaaagaacg ccaaccaatt aatggtgttg
    10561 gaaatatcat gaaaaatcgt aaatatgaag aattagatgt tccatatttt acagatagaa
    10621 cacgtgcgtc attaaaaatt caagaaggtt gtaacaactt ctgcacattc tgtattattc
    10681 catgggctcg tggcttaatg cgttcaagag atccggaaaa agtagttgaa caagcgacgc
    10741 aactagtgaa ttcaggatat aaggaaattg tattgacggg aattcataca ggtggatatg
    10801 gtcaagattt aaaagattat aacttggccc aattattacg tgatcttgaa acgattaatg
    10861 gattagaacg aattcgaatt tcttcaattg aagcaagtca acttacagat gaagtaattg
    10921 acgttttaga acgttcaaca aaagttgtgc gtcatttgca tattccatta caatctggtt
    10981 cagatacagt attaaaacgt atgagacgta agtatacaat ggatagattt tcagaacgat
    11041 taacaaaatt gcataaagct ttaccagact tggcagttac gagtgatgta attgttggtt
    11101 tcccaggtga aactgaagca gagttccaag aaacatatga ttttatcgta aagcataagt
    11161 tctctgaact gcatgttttc ccttattctc ctagaattgg cacgccagct gcaagaatgg
    11221 atgaccaaat tgatgaagaa attaaaaatg aacgcgtgca taagttaatt acgctaagca
    11281 atcaactcgg aaagttatat gcgtctaaat ttgatcaaga tgtgcttgaa gtaattcctg
    11341 aggaacaggg tgacacagaa ggtacattag ttggatatgc agataattat atgaaagtac
    11401 aatttgaagg tgacgaatca ctcataggtc aaattgtaaa agttaaaatt acgcaagcaa
    11461 attatccatt aaatgaaggg caagcaatta aagttgttga tttcgcaaca aataaatctg
    11521 atagagaagt tttagtttaa gtattccttg attcatacca taaggttccg tattaaagat
    11581 ataggttttg taacgttgtt ttaacggctt cacaatttac tatataagca actcttctca
    11641 ttaaataaat ta
//
LOCUS       DAHHSC010000064        11504 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.064, whole genome shotgun sequence.
ACCESSION   DAHHSC010000064 DAHHSC010000000
VERSION     DAHHSC010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 11504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11504
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.064"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            67..879
                     /locus_tag="NBI57_001313"
     CDS             67..879
                     /locus_tag="NBI57_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014936986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein"
                     /protein_id="HCD1822268.1"
                     /translation="MEYIKKIALYMSVLLLIIFIGGCGNMKEEQKKEANTNKTDSKEE
                     KIKKSFAKTLDMYPIKNLEELYDKEGYRDGEFEKGDKGMWTIYTDFAKSNKPGELSNE
                     GMVLYLDRNTRTAKGYYFVRTFYRKDKLPDRKNYKVEMKNNKIILLDKVEDPNLKKRI
                     ENFKFFGQYANLKELKNYSNGDVSINENVPSYDVKYKMSNKDENVKQLRSRYNIPTDK
                     SPVLKMHIDGNLKGSSVGDRKLEIDFSKRENSHLSVIDSLDYQPAKVDEDER"
     gene            869..2194
                     /locus_tag="NBI57_001314"
     CDS             869..2194
                     /locus_tag="NBI57_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001581953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1822269.1"
                     /translation="MKDKYKIDPGIIKNNTEETTAISKISYEVENANLYGADSEDITR
                     QIEYLKAKKKFPSNLEYVDSYTDSLNGVTTSAFLNKDTGKVTLGMTGTNIQGEAFKKL
                     KEGEFSRQNVTNALETVKDGYADLKILYSPASDQNYRYANTQEFINKIKSKYDIDFIT
                     GHSLGGRDAVVLGMSNGIPNIVVYNPAPISINSLNPNSPDGKRLLELYKNYKGNITRF
                     VAENDALTENLKKYKHYVFFGNDKVFKNGKGHEMEGFLTEEEQKAIKKELKKLQGYAE
                     ENNKSFVKNSNNAISKLASIELLRANMMTTNGGGLSSSQQKVLESLTALTIAQSFSQL
                     IDDEINQIKKMYNEKKKEFGKNWEDAQKAGKAVGEDLSVNGVLNALDEGQVNESSMVR
                     EPEQMISAKERQLSTIGSSVSNYIMRVRLSINEIVDKDQVLASQIGGLL"
     gene            2191..2514
                     /locus_tag="NBI57_001315"
     CDS             2191..2514
                     /locus_tag="NBI57_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1822270.1"
                     /translation="MMFNQINNKNELEESYESEKKRIENELHNLNELRHRTRKENECS
                     YDVFQYLKHEMNYSEDAQRKMTRNIEAYEQEINEIIRKQEWKLEEYKEDLKKSYEKQL
                     DKLSD"
     gene            2533..2847
                     /locus_tag="NBI57_001316"
     CDS             2533..2847
                     /locus_tag="NBI57_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1822271.1"
                     /translation="MTLIEPDMNLRMPDISTTVETLNLISKMEAQKENIRSVIAPEHK
                     HKYKDIENGLKGEEKVLIEQMAQHCEAFKANFKGAAQGDWVKSAMSEIDSIKDDLKKI
                     NS"
     gene            complement(3136..3336)
                     /locus_tag="NBI57_001317"
     CDS             complement(3136..3336)
                     /locus_tag="NBI57_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001790020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1822272.1"
                     /translation="MNMLFVILVLYVIGIAFILLSVFGSKTEGLSTKHTLYTIGSAII
                     TIAIFISIGYAIQYLTAALYGL"
     gene            3515..4717
                     /locus_tag="NBI57_001318"
     CDS             3515..4717
                     /locus_tag="NBI57_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HCD1822273.1"
                     /translation="MAKIPVTVLSGYLGSGKTTLLNHILQNREGRRIAVIVNDMSEVN
                     IDKDLVADGGGLSRTDEKLVELSNGCICCTLRDDLLKEVERLVKKGGIDQIVIESTGI
                     SEPVPVAQTFSYIDDELGIDLTAICRLDTMVTVVDANRFVHDINSEDLLMDRDQSVDE
                     TDERSIADLLIDQVEFCDVLIINKIDLISEEELAKLEKVLSALQPIAKIIKTTNSEVD
                     LKEVLNTQRFDFEKASESAGWIKELESGGHASHTPETEEYGISSFVYKRRLPFHAKRF
                     NDWLESMSNNVVRSKGIVWLAQYNHVACLLSQAGSSCNIHPVTYWVASMSEAQQTQIL
                     AERQDVAAEWDPEYGDRHTQFVIIGTELDEEKLTKELDACLVNAQEIDADWQQFEDPY
                     QWQIRPAR"
     gene            complement(5376..5438)
                     /locus_tag="NBI57_001319"
     CDS             complement(5376..5438)
                     /locus_tag="NBI57_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014532416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenol-soluble modulin PSM-alpha-4"
                     /protein_id="HCD1822274.1"
                     /translation="MAIVGTIIKIIKAIIDIFAK"
     gene            complement(5502..5570)
                     /locus_tag="NBI57_001320"
     CDS             complement(5502..5570)
                     /locus_tag="NBI57_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014373779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenol-soluble modulin PSM-alpha-3"
                     /protein_id="HCD1822275.1"
                     /translation="MEFVAKLFKFFKDLLGKFLGNN"
     gene            complement(5622..5687)
                     /locus_tag="NBI57_001321"
     CDS             complement(5622..5687)
                     /locus_tag="NBI57_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014373780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenol-soluble modulin PSM-alpha-2"
                     /protein_id="HCD1822276.1"
                     /translation="MGIIAGIIKFIKGLIEKFTGK"
     gene            complement(5719..5784)
                     /locus_tag="NBI57_001322"
     CDS             complement(5719..5784)
                     /locus_tag="NBI57_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014373781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenol-soluble modulin PSM-alpha-1"
                     /protein_id="HCD1822277.1"
                     /translation="MGIIAGIIKVIKSLIEQFTGK"
     gene            6305..7789
                     /locus_tag="NBI57_001323"
     CDS             6305..7789
                     /locus_tag="NBI57_001323"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH dehydrogenase subunit 5"
                     /protein_id="HCD1822278.1"
                     /translation="MLSFQLLFSLFVIALIIALISGLLFLAPVMPMRYIKLHLYILVM
                     PVLFAVIGFFGIHGQHVLGPFKIDRLSWLLAGFVMALGFIIQKFSMRYLLGDHHYRHY
                     FPLFTAITSFASLAWMSEDLRLMALCWGMTLLCLTLLMNVNRFWKVPRESAKLSSMTF
                     LCGWLAFVGAIVTIYIATGEWRVPQHIVHPTWSLLTNVLLVLAVMIPAAQFPFHRWLI
                     ESVTAPTPVSAIMHAGIVNAGGVILTRFAPIFDNGFALSLLLILSSISVLLGSGISLV
                     QVDYKRQLVGSTMSQMGFMLVQCALGVYSAAIIHLILHGIFKATLFLQSGSIVKRFNI
                     PKQASAKDAYGWIVMGRVLAIIVAFLFWMSSDRSAYEVLSALILAWSLLVSWNQMVAF
                     SKGRMARLVGMILIAIVTFIYVITHNYFYDVLQNITTHATTPPTVSVIISVVILIFGS
                     LLSIWVARHRYSKGFAVLYVWLVNLGEARSKAIESHPNYLKKYL"
     gene            7802..10507
                     /locus_tag="NBI57_001324"
     CDS             7802..10507
                     /locus_tag="NBI57_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbcC family protein"
                     /protein_id="HCD1822279.1"
                     /translation="MTTQLNINSVIENAKRVITPLSPISIFAARNPWEGLEADTFEDV
                     AKWLRDVRDVDIFPNKALIESAVARGELDESVFNQLVTDMLLEHHYNIPQHYINLYID
                     NIKTLKDVPASYMNHSNVDVVADLLLEKSKRDMAESYHHYDVRPMSDAIIDEQGEPLS
                     EQVNRQMIKWTKLYIDQFLSSWTMPKREQSFYHAWLHLAQHDHSFTKAQRQVIKGLPN
                     DPEMTIESVLTYFSIDQEDYQAYVEGHLLALPGWAGMLYYRSQQHHFEQHLLTDYLAI
                     RLVVEQLLVGDEFKSVTKDCESRSENWFKQTVASLCYYSDMPSDVLLQHDVNEIQTFI
                     HFAATMNKNVFKNLWLIAWEMTYESQLKQKIKAGHESVAGALDVNQVNVSENDNANQP
                     HSVLLNDTQAVDENNSELNQVGTSTKAQIAFCIDVRSEPFRRHIEAAGPFETIGIAGF
                     FGLPIQKDAVDEQFKHDSLPVMVPPAYRIKEFADRYDMNVYRQQQQTMSSMFYTFKLM
                     KNNVMPSLLLPELSGPFLSLSTIVNSIMPRKSRASLQKIKQKWLKKPETKLTIDREFD
                     RTSDLPVGFTEQEQIDFALQALKLMDLTEAFAPFVVLAGHASHSHNNPHHASLECGAC
                     GGASSGFNAKLLAMICNRPNVRQGLKQSGVYIPETTVFAAAEHHTSTDTLAWVYVPDT
                     LSALALDAYESLNDAMPMISEQANRERLDKLPTIGRVNHPVEEAQRFASDWSEVRPEW
                     GLAKNASFIIGRRQLTKGIDLEGRTFLHNYDWRKDKDGKLLNTIISGPALVAQWINLQ
                     YYASTVAPHFYGSGNKATQTVTSGVGVMQGNASDLMYGLSWQSVMAADRTMYHSPIRL
                     LVVIQAPDYVVARLFANNEHFARKVSNHWLRLMSVNEEGRFKSWI"
     gene            10669..11031
                     /locus_tag="NBI57_001325"
     CDS             10669..11031
                     /locus_tag="NBI57_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2294 domain-containing protein"
                     /protein_id="HCD1822280.1"
                     /translation="MKRTKGEIEAEISKAITQWEKDFLGRGSLSVKSDILRDMVIISL
                     QGILTPAEYRVCSTNEGLLNIKRTRSELVESGEQDLNDIILKITGIKVMSFHSDLSTV
                     TGERIIVFKLEDNLEKHI"
     gene            complement(11260..11502)
                     /locus_tag="NBI57_001326"
     CDS             complement(11260..11502)
                     /locus_tag="NBI57_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001790022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1822281.1"
                     /translation="MNRVIFVYIALIITLVRFFTPIHPSFSNLIYWIFVLYFIPIILC
                     VIGFKAEKLIATMVIIPNFLGILYRLYAYVTHILFM"
BASE COUNT         4033 a         1709 c         2244 g         3518 t
ORIGIN      
        1 actattttaa taagttatat gaaatattca aatgtttata aaattaaagc agagagatgt
       61 gaaatgatgg agtatataaa aaaaattgct ttgtacatga gtgtattact tttaatcatt
      121 tttattgggg gatgtggaaa tatgaaagaa gaacagaaaa aagaagcaaa tacaaataaa
      181 acagattcaa aagaagaaaa aatcaaaaag agttttgcga aaacgttaga tatgtatcca
      241 attaagaatc tcgaggaact atatgacaaa gaaggctatc gagatggcga atttgaaaaa
      301 ggtgacaaag ggatgtggac gatatataca gatttcgcca aaagtaataa accgggtgaa
      361 ctaagtaatg aaggcatggt cttgtactta gatagaaaca cacgcacagc taaggggtac
      421 tactttgtca ggacgtttta tcgcaaggat aaattacctg atagaaaaaa ttataaagtt
      481 gaaatgaaaa ataataaaat tatcttatta gacaaggtag aagatccaaa tctaaaaaag
      541 agaatagaaa actttaaatt tttcggacaa tatgcaaatc ttaaagaatt gaaaaactac
      601 agcaatggtg atgtctcaat taatgagaat gttccaagtt atgacgtgaa atataaaatg
      661 agtaataaag atgaaaatgt taagcaatta agaagtcgtt ataatattcc tactgataaa
      721 tcaccggtat taaaaatgca tattgatggt aatttgaaag gaagttccgt tggagatagg
      781 aagttagaaa ttgatttttc gaaacgtgaa aatagccatt tatcagtaat agattctttg
      841 gattaccagc cagcgaaggt ggatgaagat gaaagataaa tacaaaatag atccagggat
      901 tattaaaaat aatacagagg aaactacagc tatttctaaa atcagctacg aagttgaaaa
      961 tgcgaattta tatggagctg atagtgaaga tataacaaga caaatagaat atttgaaagc
     1021 caaaaaaaaa ttcccttcta atcttgaata tgttgatagt tacactgatt ctcttaatgg
     1081 agtaacaact tctgcttttt taaataaaga tacaggcaaa gtaactcttg ggatgactgg
     1141 gactaatata caaggcgaag cctttaaaaa gttaaaagaa ggtgaatttt caagacaaaa
     1201 tgttaccaat gctttggaaa cagttaaaga tggatatgca gatcttaaaa tattatattc
     1261 tcctgcatct gatcaaaact atagatatgc gaatacacaa gaatttataa ataaaataaa
     1321 aagtaagtat gacattgatt ttattactgg tcattcacta ggtggaagag atgcggtagt
     1381 tcttggaatg agtaatggta ttccgaacat tgtggtttat aatccagctc ctatttctat
     1441 aaatagtttg aatcctaatt ccccagatgg aaaacgattg ttagagttat ataaaaatta
     1501 taaaggtaat attactaggt ttgttgcaga aaatgatgca ttgacagaaa atctgaagaa
     1561 atataagcat tatgtttttt tcggtaatga taaagtcttt aaaaatggta aaggtcatga
     1621 aatggaaggc tttctgaccg aagaagaaca aaaagctata aaaaaagaac ttaaaaaact
     1681 acaaggttat gcagaagaaa ataataagtc atttgtaaag aattcaaata atgctatctc
     1741 taaattagct agtatagaat tacttagagc aaatatgatg actacaaatg gaggaggatt
     1801 atcttcttcg cagcaaaaag ttttagaaag tttaacagct ttaacaattg cgcagtcatt
     1861 cagtcaactg atagacgatg aaattaatca aatcaaaaaa atgtataatg aaaagaaaaa
     1921 ggaatttgga aaaaattggg aagacgctca aaaagctgga aaagctgtag gtgaagattt
     1981 gagtgtaaat ggagttctca atgctttaga tgaaggtcaa gtgaatgaaa gtagtatggt
     2041 aagagaacct gaacaaatga tatctgcaaa agaaagacaa ctttcaacga tagggtcttc
     2101 tgtatcaaat tatattatga gagttagact cagtattaat gaaatcgttg ataaagatca
     2161 agtgcttgca tcacaaatag gtggattact atgatgttta atcaaattaa taataaaaat
     2221 gaattagaag aatcatatga atctgagaaa aaacgtatag agaatgaact gcataattta
     2281 aatgaactta ggcatagaac tcgaaaagaa aatgaatgta gttatgatgt ttttcaatat
     2341 ttgaagcacg aaatgaatta tagtgaagat gcccaaagga aaatgacgag aaatatagaa
     2401 gcgtatgagc aagaaatcaa tgagataatt agaaagcaag aatggaaatt agaagaatat
     2461 aaagaagact taaaaaagtc ttatgaaaag cagttagata aactaagtga ctgataattt
     2521 ggaggagatt aaatgactct aatagaacca gatatgaact taagaatgcc agatataagt
     2581 actacagtag aaacacttaa tctcatatct aaaatggaag cgcaaaaaga aaacattcgt
     2641 tcagttattg cacctgaaca taagcataaa tataaagata ttgaaaacgg attaaaaggt
     2701 gaagaaaaag tattgattga acaaatggcg cagcattgcg aagcttttaa agctaatttt
     2761 aaaggtgcag ctcaaggaga ctgggttaaa agtgcaatgt ctgagataga cagtattaaa
     2821 gatgatttga aaaaaattaa tagctaaact acatatcata ctactaaata aagcgaccaa
     2881 tgttcagtat attcacaact gacactaggc cgcttctttt taatttattt tatttccatg
     2941 taaagtaacg ctctcttaat cgaaatacca cactcacaat tgtaaaaaca caacttaaat
     3001 tactgtcccg cattaaacat atctacattc caaacagcaa caacaaaatc ctcaagtacc
     3061 aaaagctatg accactttat caaaatcaac gcaagttaac ctctcccgaa ctacccgcta
     3121 tagatcatca tcaccttaca aaccataaag cgctgcagtt aagtattgaa tagcatagcc
     3181 aattgaaatg aaaatagcaa tcgttataat ggcactacca attgtatata aagtatgttt
     3241 cgtagataaa ccttccgtct tagaaccaaa aacacttagt agaataaatg caatgccaat
     3301 gacatataaa actaaaatga caaataacat gtttatacac ctcgattctc tttagtgaga
     3361 tatatctttg tctccattat aaagtaaagt gctcaataat ttaattaagt atactatttt
     3421 tttattatcg aagattgcga aagaggaaac gctcctatat aatgtaaaac ggaattattc
     3481 ctatttacaa ataaattgaa gtgagggtat atatatggct aaaattccag ttacggtatt
     3541 aagtggttat ttaggctcgg ggaaaacaac attgttaaat catattttac aaaatcgtga
     3601 aggtcgacgt atcgcggtaa ttgtaaatga tatgagtgaa gttaatatcg ataaagatct
     3661 tgtcgcagat ggtgggggac tatcgcgtac agatgaaaaa ttagtcgaac tctctaatgg
     3721 ttgtatctgt tgtacactta gagatgattt attaaaagaa gttgagcgtt tagtgaaaaa
     3781 aggtggcatc gatcaaattg ttattgagtc aacagggatt tcagagccag tacctgttgc
     3841 acaaactttc tcatatattg atgatgaact tggcattgat cttacagcga tttgccgttt
     3901 agatacaatg gttacagttg ttgatgctaa ccgcttcgta catgacatca actcagaaga
     3961 tttattgatg gatcgtgatc aaagcgttga tgaaacagat gaacgttcga ttgctgattt
     4021 attaattgac caagttgaat tttgtgatgt attgattatt aataaaattg atttaattag
     4081 tgaagaagaa cttgcaaagt tagaaaaagt gttaagcgca ttgcaaccta ttgctaaaat
     4141 tattaagaca acaaattctg aagtagattt aaaagaagtc ttaaatacgc agcgttttga
     4201 ttttgaaaaa gcgagcgagt cagcaggatg gatcaaagaa cttgagtcgg gtggtcatgc
     4261 atcgcatacg cctgaaacag aagaatatgg catatcatca tttgtatata aacgtcgtct
     4321 acctttccat gctaaaaggt tcaatgattg gttagaaagc atgtcaaata atgtcgttcg
     4381 atcaaaaggt atcgtatggt tagcacaata caatcatgta gcatgtttat tatctcaagc
     4441 agggtcatct tgcaatattc acccagttac atattgggtg gctagtatgt ctgaagcgca
     4501 acaaacacaa atattagcag aacgtcaaga tgtcgcagct gaatgggatc cagaatatgg
     4561 cgatcgtcat acacaatttg tcattattgg tacagaatta gatgaagaaa aattaacaaa
     4621 agaactcgat gcatgcttag tcaatgcgca agaaattgat gcagattggc aacaatttga
     4681 agatccatat caatggcaaa ttagaccagc acgataagtt gaatgaagta taatcatttt
     4741 tgaattgtgg cttaattgtt tgcaatttat gacaattaat aaaaagttaa gatgttctta
     4801 ggaacttttt gttttcaatc tatctaaagt gtgatagttt tgataaagca gaaatttgca
     4861 ttttatccat agggctagga catgtatgtg tcttagtcct ttttatattt gcgttgatga
     4921 aaaatgataa aaccatcgta ccaactgtta attgaaagta caattattgt ttaacggtca
     4981 taaaaatgag tgaagtaatt gcagctatca atgtactatt actgcttaca gtcacacgag
     5041 tgtttcagac taaacgtgca acgaaattct gcacgcggcc aatatatctt gcgccagatc
     5101 aagaagatca ttaagatttc gcacgccaaa tcatgaatgt cttagacttc gagtatcaaa
     5161 cataaaacac gccacaaaaa tataacgcgt cagactacaa agatcacgaa tattccttac
     5221 gtaccaccaa gaacatccta tacaaaaaat accaagacca tttatttgca acaaacaaac
     5281 aagactcacc tcacatcaat aaatcaacac aaagcaaagc caccatccct attggtatag
     5341 tggcctgaga attttaagta ttcaattcgc ttaaattatt ttgcgaaaat gtcgataatt
     5401 gctttgatga ttttaatgat agtacctaca atagccatcg ttttgtcctc ctgtatgttg
     5461 atttaacata gtgatgagtt gttgattgtt aatgtttgag attagttgtt acctaaaaat
     5521 ttaccaagta aatctttaaa gaatttgaat aattttgcta cgaattccat gtgaatggcc
     5581 cccttcaaat aagatgttca tatctatgag atttttataa cttacttacc agtgaatttc
     5641 tcaattaatc ctttaatgaa tttaatgatt cctgcaatga tacccatgtg aaagacctcc
     5701 tttgtttgtt atgaaatctt atttaccagt gaattgttcg attaagcttt tgataacttt
     5761 aatgatgcca gcgatgatac ccattaagat tacctccttt gcttatgagt taacttcatt
     5821 gtacatagtt atcttgtgcg taattgattt ttttcctaac ttgaattgtt atgatcataa
     5881 ctgcgaattt tttattttaa aaggacggga aatactgcct aactgtacag gtgcgtttac
     5941 aaaatcttta caatcattgg tttaatagag gttaagctca tgaatttgac atgaagcaga
     6001 aatttaatta tattttatgt tataagttta aaacgaataa cacgttaggt ctcgtctagg
     6061 caaagcatat ttatgaagat gaattgaaca gacagaagta acattttata tacatatgtg
     6121 catgggagca cgcttaaaga gaacactgca acaaggtata tcaacgtacc gtgatgcagt
     6181 gtctagttaa actgaaacct gcaatagtaa attttctgcc attatgtaca gaatctacta
     6241 ttgtaggttt ttatttttat acataagcaa ttatgcgaga gattagaaat aaggaggtta
     6301 tgcagtgtta agttttcaat tgctattttc actgtttgtt attgcgctta tcattgcatt
     6361 gataagtggc ttgttgtttt tagcaccagt tatgccaatg agatatatta aattacattt
     6421 atacatacta gtcatgccag tattatttgc agtcattggc tttttcggta ttcatggtca
     6481 acatgtctta ggtccattta aaatagatcg tttatcttgg ttattagctg gctttgtaat
     6541 ggcgcttggt tttattattc aaaagttttc aatgcgatat ttactaggtg atcatcatta
     6601 tagacattac tttccattgt tcactgcgat tacgtcgttt gcatctttag catggatgtc
     6661 tgaagactta agactgatgg cactctgctg gggtatgaca ttattatgtt taacactgct
     6721 gatgaacgtt aatcgttttt ggaaagtgcc acgtgagtct gcgaaattat caagcatgac
     6781 atttttatgt ggttggcttg cattcgttgg agcaattgta actatttata ttgcgactgg
     6841 tgagtggcgg gtaccacaac atatagtaca tccgacatgg tcattgttga cgaatgtact
     6901 acttgtatta gctgtcatga taccggcagc acaatttcct tttcatcgat ggttgattga
     6961 atctgtaacg gcaccaacgc cagtatcggc aattatgcat gcaggaattg tgaatgcagg
     7021 tggtgttatt ctaactcgtt ttgcgccgat atttgataat ggatttgcgt tatcattatt
     7081 acttatcctt tctagtattt ctgtattgtt aggatcgggt attagcttgg ttcaagttga
     7141 ctacaaaaga cagttagtgg gctctacgat gagtcaaatg ggctttatgt tagttcaatg
     7201 tgcattgggt gtatattcag cagcgattat tcatttaata ttgcacggta tttttaaagc
     7261 aacattattt ttacaatcag gttctatcgt gaagcgattc aatattccaa aacaagcatc
     7321 tgctaaagac gcttatggct ggattgtcat gggacgtgta ttagctatta tcgtggcatt
     7381 tctattttgg atgagtagtg acagaagtgc atatgaagtg ttaagtgcac tcattctagc
     7441 atggtcatta cttgtatctt ggaatcagat ggtagccttt agtaaagggc gcatggcacg
     7501 tctggttggc atgattttga ttgcaattgt gacatttatc tacgtcatca cacacaatta
     7561 tttttacgat gtattacaaa atataacaac acatgcgaca acaccgccta cagtgagtgt
     7621 catcattagt gttgtcattt taatctttgg cagtttatta agtatttggg tggcgcgtca
     7681 tcgatactct aagggttttg cggtattgta cgtgtggtta gttaatctag gtgaagcacg
     7741 ctcgaaagcg atagaaagtc atccgaatta tttgaagaag tatttatagg aggtgaaagg
     7801 tatgacaaca cagttaaata tcaattcagt cattgaaaat gcgaaacgtg ttattacacc
     7861 attatcaccg atttcgattt ttgcagcacg taacccctgg gaaggattag aagcggatac
     7921 gtttgaagat gtcgcaaaat ggttacgtga cgttcgcgat gtggatattt tcccaaataa
     7981 agcattaata gaaagcgctg tggcacgtgg tgaattagat gaaagtgtct ttaatcaact
     8041 tgttactgat atgttacttg aacatcacta caatatcccg caacactaca tcaatcttta
     8101 tattgataac attaaaacat taaaagacgt acctgcatca tatatgaatc attcaaatgt
     8161 tgatgttgtt gctgatctac tattagaaaa atcaaaacgt gatatggctg aatcatatca
     8221 tcactatgat gtacgtccga tgagtgatgc aataatagat gaacaaggtg agccacttag
     8281 cgaacaagtg aatcgtcaaa tgattaaatg gacgaaactt tatatcgatc aatttctatc
     8341 gagttggaca atgccgaagc gtgagcaaag tttttaccat gcatggttgc atttagcgca
     8401 acatgaccat agttttacta aagcacagcg ccaagtgatt aaaggcttac ccaatgatcc
     8461 tgaaatgacg atagagtcag tattaactta tttttcaata gatcaggaag actaccaagc
     8521 ttatgttgaa ggacatcttt tggcgttacc gggttgggca ggtatgttgt attaccgttc
     8581 acaacagcat cactttgaac aacatttgtt aacggattat ttggcaattc ggttagttgt
     8641 cgaacaattg ctagttggtg atgagtttaa gtcagtcact aaagattgtg aaagtagatc
     8701 ggaaaattgg tttaagcaaa ctgttgcatc attgtgttac tacagtgata tgcctagcga
     8761 tgtattacta caacatgacg tcaatgaaat tcaaacgttt attcattttg cagcaactat
     8821 gaataaaaat gtatttaaaa atttatggct aattgcctgg gaaatgacat acgaatctca
     8881 gttaaaacaa aaaattaaag caggtcatga aagtgtggcg ggcgcattag atgtaaacca
     8941 agtaaatgtc tcagaaaatg ataacgctaa tcagccacat tcagtattgt taaatgacac
     9001 acaagcagtt gatgaaaata atagcgagct aaatcaggtg ggcacatcaa cgaaagcgca
     9061 aattgcattt tgtatagatg ttcgttcaga accatttcgt agacatatcg aagcagcagg
     9121 gccctttgaa acgattggta ttgcaggttt ctttggatta cctattcaaa aagatgccgt
     9181 agacgaacaa ttcaaacatg attcattacc tgtcatggta ccgccggcat atcgcattaa
     9241 agaatttgca gaccgctacg atatgaatgt ttatcgacaa cagcaacaga caatgtcatc
     9301 gatgttttac acatttaaat tgatgaaaaa taatgttatg cctagtctgt tattgcctga
     9361 attaagtggg ccatttttaa gcttgagtac cattgtcaat tcgattatgc ctagaaaaag
     9421 tcgcgcgtct ttacaaaaaa taaaacaaaa atggttgaaa aagcctgaaa caaagttaac
     9481 gattgatcgt gagtttgacc gaacatcaga cttacctgtt ggatttactg agcaagagca
     9541 aattgatttc gcgttacaag cgttgaaatt gatggattta accgaagcat ttgcgccgtt
     9601 cgttgtgtta gcaggtcatg ctagtcattc tcacaataat ccacatcatg catcacttga
     9661 atgtggggct tgtggtggcg catcaagcgg ttttaatgct aagttattag cgatgatatg
     9721 taatcgtcca aatgtcagac aaggattaaa acaatcaggt gtgtatattc cagagacaac
     9781 tgtttttgcg gcagcagaac atcatacgtc tactgatacg ttggcatggg tatatgtgcc
     9841 agacacatta tcagctttag ctctagatgc atatgaatca ttgaatgacg cgatgccgat
     9901 gatttctgaa caagcgaatc gcgaacgttt ggacaaactg ccaacgattg gtcgtgtgaa
     9961 tcatccagtg gaagaagcac agcggtttgc aagtgattgg agtgaggtac gtccagaatg
    10021 gggcttggct aaaaatgcat catttataat tggacgacgc caattaacaa aaggcattga
    10081 tttagaaggg cggacatttt tacacaatta tgattggcgt aaagataaag atggcaaatt
    10141 gttaaatacc atcatttctg gtccggcgct tgtggcacaa tggattaatt tacaatatta
    10201 tgcgtcgaca gttgcgccgc atttttacgg aagtgggaat aaagcgacac aaaccgtcac
    10261 gtcaggtgtt ggtgtcatgc aaggtaatgc gagtgatctg atgtatggct tatcatggca
    10321 atctgttatg gctgctgatc ggacgatgta tcattcgcca attcgtttac ttgtcgttat
    10381 tcaggcaccc gactatgttg tagctagact attcgcgaat aatgagcatt tcgctaggaa
    10441 ggtgtctaat cattggctgc gtttaatgag cgttaatgag gaagggcgtt ttaaaagttg
    10501 gatttaacga tgatatgttg cgatgctaag taattaatat atcatcatat ctacaatgtt
    10561 ctagcttctt aaaatatact aaaaatgata aacttaatgt aatagttgat gtcgtgataa
    10621 cgatacaaag atgaagtaat caattttaat attattggag ttagtgatat gaaaagaacg
    10681 aaaggtgaaa tcgaagctga aatcagtaaa gccattacgc aatgggaaaa agatttcctt
    10741 ggcagaggtt ccttgtcagt taaatcagac attttaagag atatggtgat tattagttta
    10801 caaggtatct taacgccagc agaatatcgt gtgtgtagta cgaatgaagg attactaaat
    10861 attaaacgaa cacgttctga attagttgaa tccggtgagc aagatttgaa tgatatcatt
    10921 cttaaaatta caggtatcaa agtgatgagc ttccacagtg atttaagtac agttacaggt
    10981 gaacgtatta tcgtattcaa acttgaggat aatttggaaa agcatattta agaaggttag
    11041 gtatgcttct gctggcttat taaagctata gaagtatgct tggttaaaca gcgcaacgcc
    11101 atatatttgg gtgtgctgtt tttctattac atgatgtgtt tattacgtgg atgaatgcgt
    11161 gggtctgaat atagcccgtg aaaggcgtat gtcataaaat ttgcgaatgg tgacaagtca
    11221 acgcaagcat atttgttgcg agacatgccc actatccttt tacataaaga gtatatgtgt
    11281 gacgtaggca tataatcgat aaagtatacc taaaaaatta ggtataatga ccattgttgc
    11341 aataagtttt tcggccttga aaccgataac gcatagtata ataggaataa aatataatac
    11401 aaatatccag taaatcaaat ttgaaaacga tggatgaata ggggtaaaga atctaactaa
    11461 tgtaatgatt aacgctatat agacaaaaat aactctattg atac
//
LOCUS       DAHHSC010000065        11430 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.065, whole genome shotgun sequence.
ACCESSION   DAHHSC010000065 DAHHSC010000000
VERSION     DAHHSC010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 11430)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11430)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11430
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.065"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            84..2717
                     /gene="clfB"
                     /locus_tag="NBI57_001327"
     CDS             84..2717
                     /gene="clfB"
                     /locus_tag="NBI57_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MSCRAMM family adhesin clumping factor ClfB"
                     /protein_id="HCD1822282.1"
                     /translation="MKKRIDYLSNKQNKYSIRRFTVGTTSVIVGATILFGIGNHQAQA
                     SEQSNDTTQSSKNNASADSEKNNMIETPQLNTTANDTSDISANTNSANVDSTTKPMST
                     QTSNTTTTEPASTNETPQPTAIKNQATAAKMQDQTVPQEANSQVDNKTTNDANSIATN
                     SELKNSQTLDLPQSSPQTISNAQGTSKPSVRTRAVRSLAVAEPVVNAADAKGTNVNDK
                     VTASNFKLEKTTFDPNQSGNTFMAANFTVTDKVKSGDYFTAKLPDSLTGNGDVDYSNS
                     NNTMPIADIKSTNGDVVAKATYDILTKTYTFVFTDYVNNKENINGQFSLPLFTDRAKA
                     PKSGTYDANINIADEMFNNKITYNYSSPIAGIDKPNGANISSQIIGVDTASGQNTYKQ
                     TVFVNPKQRVLGNTWVYIKGYQDKIEESSGKVSATDTKLRIFEVNDTSKLSDSYYADP
                     NDSNLKEVTDQFKNRIYYEHPNVASIKFGDITKTYVVLVEGHYDNTGKNLKTQVIQEN
                     VDPVTNRDYSIFGWNNENVVRYGGGSADGDSAVNPKDPTPGPPVDPEPSPDPEPEPTP
                     DPEPSPDPEPEPSPDPDPDSDSDSDSGSDSDSGSDSDSESDSDSDSDSDSDSDSDSES
                     DSDSESDSDSDSDSDSDSDSDSESDSDSDSDSDSDSDSDSESDSDSESDSESDSDSDS
                     DSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDS
                     DSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDS
                     DSDSDSRVTPPNNEQKAPSNPKGEVNHSNKVSKQHKTDALPETGDKSENTNATLFGAM
                     MALLGSLLLFRKRKQDHKEKA"
     gene            2833..3594
                     /locus_tag="NBI57_001328"
     CDS             2833..3594
                     /locus_tag="NBI57_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HCD1822283.1"
                     /translation="MAYISLNYHSPTIGMHQNLTVILPEDQSFFNNDITAKPLKTLML
                     LHGLSSDETTYMRYTSIERYANEHKLAVIMPNVDHSAYANMAYGHSYYDYILEVYDYV
                     HQIFPLSKKRDDNFIAGHSMGGYGTIKFALTQGDKFAKAVPLSAVFEAQNLMDLEWND
                     FSKEAIIGNLSSVKGTEHDPYYLLDKAVAEDKQIPKLLIMCGKQDFLYQDNLDFIDYL
                     SRKNVPYQFEDGPGDHDYAYWDQAIKRAITWMVND"
     gene            3839..4294
                     /locus_tag="NBI57_001329"
     CDS             3839..4294
                     /locus_tag="NBI57_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HCD1822284.1"
                     /translation="MSTNKNDYEHMLFYFAYKTFITTADEIIEKYGMSRQHHRFLFFI
                     NKLPGITIKSLLEILEISKQGSHATLQKLKEQGLIIEKVLETDRRVKKLYSTDKGDQL
                     IAELNKAQDELLQNIYQQVGSDWYDVMEALAKGRPGFDFIKHLKDEKES"
     gene            complement(4397..4507)
                     /locus_tag="NBI57_001330"
                     /pseudo
     CDS             complement(4397..4507)
                     /locus_tag="NBI57_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501411.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(4577..6001)
                     /locus_tag="NBI57_001331"
     CDS             complement(4577..6001)
                     /locus_tag="NBI57_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HCD1822285.1"
                     /translation="MLSVKKATKFAVANLIIVSTVFSSVTPAIAQSDKSSKDESFAVG
                     NTKNPKNVIFLVGDGMGPSFNTAYRYYKNDSHAKEMTPTAFDSYLKGTNRTYSNDPKQ
                     NITDSAAGGTAFSSGHKTYNGAIGVDSNKQKVKTVLERAKEKGKSTGLVSTAELTDAT
                     PAAYAAHVTSRDDKNEIAKQFYKDKINGKHKVDVLLGGGAKYFGKSNGNLDKKFKKDG
                     YDLATNSKDLSKSDKDKVLGLFADKNMPLAIDASKDEPSLADMQQSALSKLERNKKGF
                     FLMVEGASIDKSAHSNDITGVMSEMEGFEKAFDDAIQYAKKHKDTLVVATADHSTGGL
                     TIGKDKGYEWNPQPIKSMKHSGSYMTEKMAKGEDPEKVINEGYGFEFSQDDMKKVKKE
                     NKKLQKLLKKEKDEKSADVEKQTKALQHAIQKPVNDKSYTGWTSDGHTGEDVNTYAYG
                     PRSESFSGNIENTESAKIIFDIFK"
     gene            6199..6399
                     /locus_tag="NBI57_001332"
     CDS             6199..6399
                     /locus_tag="NBI57_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1822286.1"
                     /translation="MILVMLSPLLIIFFIVLSILEERKRTKKKQLEKEKANTLNQNTN
                     DTESSNQEPSLQQTKEQKDNKG"
     gene            6425..7090
                     /gene="nsaR"
                     /locus_tag="NBI57_001333"
     CDS             6425..7090
                     /gene="nsaR"
                     /locus_tag="NBI57_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nisin susceptibility-associated two-component
                     system response regulator NsaR"
                     /protein_id="HCD1822287.1"
                     /translation="MKILIVEDDFVIAESLASELKKWNYGVIVVEQFDDILSIFNQHQ
                     PQLVLLDINLPTLNGFHWCQEIRKTSNVPIIFISSRIDNMDQIMAIQMGGDDFIEKPF
                     NLSLTIAKIQALLRRTYDLSVANDSLTVKGCTLILDEAKVVYQEQNIQLSLTELQILK
                     LLFQNEDKYVSRTALIEKCWESENFIDDNTLAVNMTRLRKKLNTIGVNDFIITKKNVG
                     YKV"
     gene            7101..7988
                     /gene="nsaS"
                     /locus_tag="NBI57_001334"
     CDS             7101..7988
                     /gene="nsaS"
                     /locus_tag="NBI57_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nisin susceptibility-associated two-component
                     system sensor histidine kinase NsaS"
                     /protein_id="HCD1822288.1"
                     /translation="MTFLKSITQEIAIVIVIFALFGLMFYLYHLPLEAYLLALGVILL
                     LLLIFIGIKYLSFVKTISQQQQIENLETALYQLKNEQIEYKNDVESYFLTWVHQMKTP
                     ITAAQLLLERDEPNVVNRVRQEVIQIDNYTSLALSYLKLLNETSDISVTKISINNIIR
                     PIIMKYSIQFIDQKTKIHYEPCHHEVLTDVRWTSLMIEQLINNALKYARGKDIWIEFD
                     EQSNQLHVKDNGIGISEADLPKIFDKGYSGYNGQRQSNSSGIGLFIVKQISTHTNHPV
                     SVVSKQNEGTTFTIQFPDE"
     gene            8096..8851
                     /locus_tag="NBI57_001335"
     CDS             8096..8851
                     /locus_tag="NBI57_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCD1822289.1"
                     /translation="MLLEVKHVKKVYGKGLNATTALNQMNLSVGAGEFVAIMGESGSG
                     KSTLLNLIASFDGLTEGDIIVDGAHLNNMKNKSKALYRQQMVGFVFQDFNLLPTMTNK
                     ENIMMPLILAGAKRKDIEQRVHQLAVQLHLEGFLNKYPSEISGGQKQRIAIARALVTK
                     PTILLADEPTGALDSKTSKALMMLFQEIHQLEQTILMVTHSNIDASYAERVIFIKDGR
                     LYHEIYRGEESQLAFQQRITDSLALVNGGSVNI"
     gene            8848..10848
                     /locus_tag="NBI57_001336"
     CDS             8848..10848
                     /locus_tag="NBI57_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCD1822290.1"
                     /translation="MKLRLLWHIVRRQFITQRLVIIPFILAVSVLFMIEYTLVSIGLN
                     SYIKQKNDFLVPFIIIANFFMALLTFIFIFYANHFMMSQRRKEFSIFMTLGMTKKSMR
                     LIVVMETILQFVIISVVSIAGGYLLGAIFFLFIQKIMGSEVATLRYYPFDSVAMFITL
                     IIIAVLMGMLLIFNLFSINFQRPITYQHRSDSSVISRWLRYVLIVIGSAALYLGYFIA
                     LQQDTTFGAFFKIWIVIGLVIIGTYAFFVGISEIIISILQQVSKVYYHPRYFFVVVGM
                     RVRLKMNAVSLATITLLCTFLIVTLTMTLTTYRDMNHTITKLITNDYDLSFSDNSKSQ
                     IERQQTIENIKRDIQGSVNAKDFKVYETTLFRASLEKNRAYYKLKQASDDIPIDFIGN
                     EGAIFSRQSVMITAFTAQDYNRYHQNKLHLDNQSIGMITNVPIFKKQSKVGLNNEVFK
                     VKQLDAHALNLMMIQDSMVLIVKDNNQLQKVKGFYAHEQKNSTISINFNVFGKTKLTT
                     SQIQKLSKKYDASINSKSEMLMVWDRLTGGLIFVGGVVSIVLVIGIFLMMYYKQVSEA
                     YANQHNYDIMKKLGLDNGRIAKITRNQMTFLFAIPITVALIHTLISSNIVYTLLNMIG
                     INNHHIFLTSYVLAVIIISFLYMAMYKITSYIYAKVIHQQRN"
BASE COUNT         4083 a         1775 c         1992 g         3580 t
ORIGIN      
        1 atttttttcg aaaagcaatt gatataattc ttatttcatt atacaattta gactaatcta
       61 gaaattgaaa tggagtaata tttttgaaaa aaagaattga ttatttgtcg aataagcaga
      121 ataagtattc gattagacgt tttacagtag gtaccacatc agtaatagta ggggcaacta
      181 tactatttgg gataggcaat catcaagcac aagcttcaga acaatcgaac gatacaacgc
      241 aatcttcgaa aaataatgca agtgcagatt ccgaaaaaaa caatatgata gaaacacctc
      301 aattaaatac aacggctaat gatacatctg atattagtgc aaacacaaac agtgcgaatg
      361 tagatagcac aacaaaacca atgtctacac aaacgagcaa taccactaca acagagccag
      421 cttcaacaaa tgaaacacct caaccgacgg caattaaaaa tcaagcaact gctgcaaaaa
      481 tgcaagatca aactgttcct caagaagcaa attctcaagt agataataaa acaacgaatg
      541 atgctaatag catagcaaca aacagtgagc ttaaaaattc tcaaacatta gatttaccac
      601 aatcatcacc acaaacgatt tccaatgcgc aaggaactag taaaccaagt gttagaacga
      661 gagctgtacg tagtttagct gttgctgaac cggtagtaaa tgctgctgat gctaaaggta
      721 caaatgtaaa tgataaagtt acggcaagta atttcaagtt agaaaagact acatttgacc
      781 ctaatcaaag tggtaacaca tttatggcgg caaattttac agtgacagat aaagtgaaat
      841 caggggatta ttttacagcg aagttaccag atagtttaac tggtaatgga gacgtggatt
      901 attctaattc aaataatacg atgccaattg cagacattaa aagtacgaat ggcgatgttg
      961 tagctaaagc aacatatgat atcttgacta agacgtatac atttgtcttt acagattatg
     1021 taaataataa agaaaatatt aacggacaat tttcattacc tttatttaca gaccgagcaa
     1081 aggcacctaa atcaggaaca tatgatgcga atattaatat tgcggatgaa atgtttaata
     1141 ataaaattac ttataactat agttcgccaa ttgcaggaat tgataaacca aatggcgcga
     1201 acatttcttc tcaaattatt ggtgtagata cagcttcagg tcaaaacaca tacaagcaaa
     1261 cagtatttgt taaccctaag caacgagttt taggtaatac gtgggtgtat attaaaggct
     1321 accaagataa aatcgaagaa agtagcggta aagtaagtgc tacagataca aaactgagaa
     1381 tttttgaagt gaatgataca tctaaattat cagatagcta ctatgcagat ccaaatgact
     1441 ctaaccttaa agaagtaaca gaccaattta aaaatagaat ctattatgag catccaaatg
     1501 tagctagtat taaatttggt gatattacta aaacatatgt agtattagta gaagggcatt
     1561 acgacaatac aggtaagaac ttaaaaactc aggttattca agaaaatgtt gatcctgtaa
     1621 caaatagaga ctacagtatt ttcggttgga ataatgagaa tgttgtacgt tatggtggtg
     1681 gaagtgctga tggtgattca gcagtaaatc cgaaagaccc aactccaggg ccgccggttg
     1741 acccagaacc aagtccagac ccagaaccag aaccaacgcc agatccagaa ccaagtccag
     1801 acccagaacc ggaaccaagc ccagacccgg atccggattc ggattcagac agtgactcag
     1861 gctcagacag cgactcaggt tcagatagcg actcagaatc agatagcgat tcggattcag
     1921 acagtgattc agattcagac agcgactcag aatcagatag cgattcagaa tcagatagcg
     1981 actcagattc agatagcgat tcagattcag atagcgattc agaatcagat agcgattcgg
     2041 attcagacag tgattcagat tcagacagcg actcagaatc agatagcgac tcagaatcag
     2101 atagtgagtc agattcagac agtgactcgg actcagacag tgattcagac tcagatagcg
     2161 attcagactc agatagcgat tcagactcag acagcgattc agattcagac agcgactcag
     2221 actcagacag cgactcagac tcagatagcg actcagactc agacagcgac tcagattcag
     2281 atagcgattc agactcagac agcgactcag actcagacag cgactcagac tcagatagcg
     2341 attcagactc agacagcgac tcagattcag atagcgattc ggactcagac agcgattcag
     2401 attcagacag cgactcagac tcggatagcg attcagattc agacagcgac tcagactcgg
     2461 atagcgactc ggattcagat agtgactccg attcaagagt tacaccacca aataatgaac
     2521 agaaagcacc atcaaatcct aaaggtgaag taaaccattc taataaggta tcaaaacaac
     2581 acaaaactga tgctttacca gaaacaggag ataagagcga aaacacaaat gcaactttat
     2641 ttggtgcaat gatggcatta ttaggatcat tactattgtt tagaaaacgc aagcaagatc
     2701 ataaagaaaa agcgtaaata cttttttagg ccgaatacat ttgtattcgg tttttttgtt
     2761 gaaaatgatt ttaaagtgaa ttgattaagc gtaaaatgtt gataaagtag aattagaaag
     2821 gggtcatgac gtatggctta tatttcatta aactatcatt caccaacaat tggtatgcat
     2881 caaaatttga cagtcatttt accggaagat caaagcttct ttaataacga tataactgct
     2941 aaaccattaa aaactttaat gttgttacat ggattatcaa gtgatgaaac gacatatatg
     3001 agatatacaa gcatagaaag gtatgcgaat gaacacaaat tagctgtgat tatgcccaat
     3061 gtggatcata gcgcatatgc taacatggca tatggtcata gctattatga ttatattttg
     3121 gaagtatatg attatgttca tcaaatattt ccactttcca aaaagcgtga tgacaatttt
     3181 atagcaggtc actctatggg aggatatggc acaattaaat ttgcattaac acaaggggat
     3241 aaatttgcca aagctgtacc attatctgct gtgtttgaag cgcaaaattt aatggatcta
     3301 gagtggaatg atttttcaaa agaggccata attggcaatc tttcaagtgt taaaggaact
     3361 gaacatgatc cgtattattt gctagacaaa gctgtagctg aagataaaca aattccaaaa
     3421 ttgctcatta tgtgtggtaa acaagacttt ttatatcaag acaacttaga ttttatcgat
     3481 tatttatcac gtaaaaatgt tccttatcaa tttgaagatg gaccaggaga tcatgattat
     3541 gcatattggg accaagcgat taagcgtgct ataacatgga tggtgaatga ttaattattt
     3601 cctggaaaat atgtggttgc attaaataca cagagtgaga gatacaaact atttacgcac
     3661 gactaacatt tctgagtgtt taaattaaat ttgtattaat atgattggcg caatttgctg
     3721 atacgcaaaa atgtttctcg tgaaacttag atttagctta tagttttatc atcatttgta
     3781 tgacttacat tataaatttt attataatga ggttaacgct ttgaaaggag tcatcatcat
     3841 gtcgaccaat aaaaacgatt atgagcatat gttgttttat tttgcatata aaacgtttat
     3901 tactaccgct gatgaaatta tagagaagta tggtatgagc cgtcagcatc atcgtttttt
     3961 gttttttatc aataaattac ctggtattac tattaaatca ttactagaaa tattagaaat
     4021 ttctaaacaa ggatcacatg caacacttca aaaattaaaa gagcaaggtc tcattattga
     4081 aaaagtttta gagactgatc gacgtgtcaa aaaattatat tcgacggata aaggcgatca
     4141 actcattgct gaattgaaca aggcgcaaga tgaattattg caaaatatat atcaacaagt
     4201 cggttcggat tggtatgatg tgatggaagc attggctaaa gggcgacctg gctttgattt
     4261 tattaagcat ttgaaagatg aaaaagaaag ctagcatcag aaatgttaaa aatcttcgca
     4321 ttcttaaatt taaaaaatat gtcaaaaagt gtataataaa aacatataat ttaattgaac
     4381 tcagtttcaa cacatcttag aaaggagttt gaatgatgaa aaaattagca gttattttaa
     4441 cattagttgg cggtttatac tacgcattta aaaaatacca agaacgtgtt aaccaagcac
     4501 ctaacattga gtactaaatt aaaccataaa aaattcccga acaccttgtt atagtgctcg
     4561 ggaatttttt tatgctttac ttgaatatat caaatattat ttttgcgctt tctgtatttt
     4621 cgatattacc actaaatgat tctgatctag gtccgtaagc gtaggtatta acatcctcgc
     4681 ctgtatgtcc atcggaagtc caccctgtat aagatttatc atttactggc ttctgaattg
     4741 cgtgttgtaa ggcttttgtt tgtttttcta catctgcaga tttttcgtct ttttcttttt
     4801 taagtaattt ttgcaacttt ttattctctt ttttaacctt tttcatatca tcttgtgaaa
     4861 attcaaagcc ataaccttca ttaataactt tttcagggtc ttcaccttta gccatttttt
     4921 ctgtcatata tgatccagag tgtttcatcg atttaatcgg ttgaggattc cattcgtatc
     4981 ctttatcttt accaattgtt agaccaccag tagagtgatc tgctgttgca acgacaagtg
     5041 tatctttatg tttcttagca tattgaatgg cgtcatcgaa tgctttttca aaaccttcca
     5101 tttcagacat aacgcctgta atatcgttgg aatgcgctga tttatctata gaagcacctt
     5161 cgaccattaa aaagaaacct tttttattac gctcaagctt actaagtgca ctttgttgca
     5221 tatcagctaa tgatggttcg tctttagaag catctattgc aagtggcata tttttatctg
     5281 caaacaaacc aagaacttta tctttatcag attttgataa atccttactg ttcgtggcaa
     5341 ggtcgtaacc atcttttttg aattttttat ctaaattgcc attactttta ccgaaatatt
     5401 tagcgccgcc gcctaataaa acatcaactt tatgctttcc gttgatttta tctttataaa
     5461 attgtttagc gatttcgttt ttatcatctc tagaagtcac gtgtgcagca tatgctgctg
     5521 gtgttgcatc tgttaattca gctgttgaaa caagaccagt cgacttacct ttttcttttg
     5581 cacgttcaag cactgtcttt actttctgct tgttactgtc aacaccgatg gcaccattat
     5641 atgtcttatg gccagaacta aaggctgttc cgccagctgc agaatcagta atattctgtt
     5701 ttgggtcatt tgaatatgta cgatttgtgc cttttaaata tgaatcaaaa gcagtagggg
     5761 tcatttcttt ggcatgcgaa tcatttttat aataacgata agctgtgtta aatgatggac
     5821 ccatgccatc gccaactaaa aagataacat tttttggatt tttagtatta ccaaccgcga
     5881 aactttcatc tttagaactt ttatcggatt gcgcaattgc aggtgtgaca gaactgaaaa
     5941 ctgttgacac gataataagg ttagcaactg caaattttgt ggctttttta actgataaca
     6001 taatacatcc tcctgagtat atgactatgt cttcagtgta aaagaggaat tttgagcaat
     6061 tatgtagttt tagttaaaaa tatgtaaaca gagtgattta gaataacaaa aaatgaatat
     6121 atatgacaat ttgttataga aagcgttaga atagaagcgt gtgaaaatat agaattaaat
     6181 ataatttgag gtggaaaaat gatactagta atgttatctc cattattaat catattcttt
     6241 atagtgttgt ctattttaga ggagcgtaaa cgtacgaaga aaaagcaact cgagaaagaa
     6301 aaagcaaata cactaaatca aaatacaaat gacacggaaa gttcaaatca agagccgtca
     6361 ttgcagcaga ctaaagaaca aaaagataac aaaggataat tcaattgaag gaagaagatt
     6421 atagatgaaa atattaattg ttgaagatga ttttgttata gcagaaagtt tagcatctga
     6481 acttaaaaaa tggaattacg gtgttattgt cgttgaacaa tttgatgata tactgtctat
     6541 ctttaaccaa catcaacctc agcttgtatt gctagatatt aatttgccaa cgttaaatgg
     6601 ttttcattgg tgtcaagaaa tccgaaaaac atctaatgtg ccaattatat ttattagttc
     6661 ccgtattgat aatatggacc aaattatggc aatacaaatg gggggagatg attttatcga
     6721 aaagccattt aacttgtcat taacgattgc caaaattcaa gcattattga gacgaactta
     6781 tgacttgtca gtagctaatg attcattgac ggttaaaggt tgtacattaa tattagatga
     6841 agcaaaagtc gtgtatcaag aacaaaacat acagctatct ttgactgaat tacaaatatt
     6901 aaagttatta tttcaaaatg aagataaata tgtaagtaga actgctttaa ttgaaaaatg
     6961 ttgggaatca gaaaacttca tagatgataa cacattagct gttaacatga cgcgcctgcg
     7021 taaaaaatta aatactattg gcgttaatga ttttatcatt acaaagaaaa atgtcggata
     7081 taaagtatag ggtgaatgca atgacctttc ttaaaagtat tactcaggaa atagcaatag
     7141 tcatagttat ttttgcttta tttggcttaa tgttttacct gtatcatttg ccattagaag
     7201 catatttact agcacttggc gttattttat tattattact catattcata ggtattaaat
     7261 atttaagttt tgtaaaaact ataagccaac aacaacaaat tgaaaactta gaaactgcgt
     7321 tgtatcagct taaaaatgaa caaattgaat ataaaaatga tgtagagagc tactttttaa
     7381 catgggtaca tcaaatgaaa acacccatta ctgcagcaca actgttactt gaaagagatg
     7441 agcctaatgt tgttaatcgt gttcgtcaag aggttattca aattgataac tatacaagtt
     7501 tagcacttag ttatttaaag ttattaaatg aaacttctga tatttctgtc actaaaattt
     7561 cgattaataa tatcattcgc ccaattatta tgaaatattc aatacagttt attgatcaaa
     7621 aaacaaaaat ccattatgaa ccttgtcatc acgaagtatt aactgacgtt agatggacct
     7681 ctttaatgat agaacaatta ataaataatg cacttaagta tgcgagaggt aaagatatat
     7741 ggattgaatt tgatgagcaa tccaatcaat tacacgtaaa agataatggt atcggtatta
     7801 gtgaagcgga cttgcctaaa atatttgata agggctattc aggttataat ggccagcgcc
     7861 aaagtaactc aagtgggatt ggtttattta tcgtaaaaca aatttcaaca cacacaaacc
     7921 atcctgtttc agtcgtatct aaacaaaatg agggtacaac atttacgatt caatttccag
     7981 atgaataaaa actttcaata ttgtaagtat actagtaaca tttttttact aatttaaatg
     8041 ttattagtat ttttttgttt taatatagaa ctaacaaaga aatgaggtgc atgccatgtt
     8101 gctagaagtg aaacatgtaa aaaaggttta tggtaaaggt ttgaatgcta cgacagcact
     8161 taatcaaatg aatttatcag ttggagctgg tgaatttgtt gcaattatgg gtgagtctgg
     8221 gtcagggaag tctacactac taaatttaat tgcttctttt gatggactaa ctgaaggtga
     8281 cattattgtg gatggcgcac atttaaataa tatgaaaaat aaaagtaaag cattgtatcg
     8341 tcaacaaatg gtaggttttg tttttcaaga ttttaatctt ttaccaacaa tgacgaataa
     8401 agaaaatata atgatgccat taattttagc tggtgctaaa cgaaaagata tagaacaaag
     8461 ggtacatcag ttggcagtac aattacattt agagggattc ttaaacaagt atccttctga
     8521 aatctctggg ggtcagaagc aacgcattgc cattgcacgt gcattagtta ctaagccgac
     8581 gattttacta gccgatgaac ctacaggtgc acttgattca aaaacgtcaa aggcattgat
     8641 gatgttattt caagagattc atcaattgga acagacaatt ttaatggtaa ctcattcaaa
     8701 tatcgatgcg tcttatgcag agcgagtcat ttttattaaa gatgggcgtc tatatcatga
     8761 aatatatcgt ggtgaagaaa gtcaattagc ttttcaacaa cgaataacag atagcttagc
     8821 acttgtgaat ggaggaagtg tcaatatatg aagttaagat tgttatggca catagtgcga
     8881 cgtcaattta ttacgcagcg acttgtaatc attccattca ttttagcggt aagtgtacta
     8941 ttcatgattg aatatacgct tgtgtcaatt gggttaaata gctacataaa acagaagaat
     9001 gacttcctag taccatttat tatcatagct aattttttta tggcgctttt aacttttatt
     9061 tttattttct atgcaaatca ctttatgatg tcacaaagac gaaaagagtt tagcattttt
     9121 atgacattgg gcatgaccaa gaaaagtatg cgtttaattg tagtgatgga aactatctta
     9181 caatttgtga taatttcagt cgttagtatt gccggcggat acttacttgg tgcgatattt
     9241 ttcttgttta tacagaaaat aatgggcagt gaagttgcga cgttaaggta ttatccattt
     9301 gactctgtag cgatgtttat tactttgatt atcattgctg tattaatggg catgctactt
     9361 atattcaact tgttcagtat taattttcaa cggccgataa cttatcaaca tcgttccgat
     9421 tctagtgtca tatcacgatg gttgcgttac gttttaattg ttataggaag cgcagcacta
     9481 tatttaggtt actttattgc attacaacaa gatacgacgt ttggtgcctt ttttaaaata
     9541 tggattgtca taggattagt tattatcggt acttatgcat tttttgtagg tataagtgaa
     9601 ataattatta gtatattgca gcaggtatca aaagtttact atcatccacg gtattttttt
     9661 gtggtagttg ggatgcgtgt acgtcttaaa atgaatgcag tcagtcttgc aacaatcact
     9721 ttgctgtgta catttttgat tgtaacgctc acaatgacat taacaaccta tcgtgatatg
     9781 aatcatacca ttacgaaatt gattacgaat gattatgatt tgtcatttag cgacaattct
     9841 aagtcacaaa tagaacgtca acaaacaatt gagaatatta aacgtgatat acagggttcg
     9901 gttaatgcaa aagacttcaa agtatatgaa acgacattat ttagagcttc tttagaaaag
     9961 aatcgtgctt attacaagtt aaaacaggct agtgatgata ttccaataga tttcattggt
    10021 aatgaaggcg cgatattttc aagacaatca gtaatgatta cagcttttac tgcacaagat
    10081 tataatcgat atcaccaaaa caaattacac ttagacaatc aaagtatagg tatgattacg
    10141 aatgtaccta tttttaaaaa gcagtctaag gtcggtctta ataatgaagt ctttaaagtg
    10201 aaacaacttg acgcacatgc gttaaatttg atgatgattc aagatagtat ggttttaatt
    10261 gttaaagata acaatcaatt acaaaaagta aaaggatttt atgcacatga acaaaagaat
    10321 tcgacaatat cgataaactt taatgtattt ggaaagacga aattaacaac atcacaaata
    10381 caaaagctat cgaaaaaata cgatgcgtca ataaattcta aatcagaaat gctaatggtt
    10441 tgggatcgtt tgacaggtgg actaatattt gtaggtggcg tagtatcaat tgtacttgtc
    10501 attggaatat ttctaatgat gtactataaa caagtctcag aagcctatgc aaatcagcac
    10561 aattatgaca ttatgaaaaa acttggttta gataatggaa gaatagcaaa gataacacga
    10621 aatcaaatga ctttcttgtt cgcaatacca ataacagtag cactgattca cacattaatt
    10681 tctagtaaca ttgtatatac gttactaaat atgataggta ttaataacca tcatatattt
    10741 ttaacaagtt acgtactagc tgtcattata atttcgtttc tatatatggc gatgtacaaa
    10801 ataacttcct atatttatgc taaagttatt catcaacaac gtaattaacc atgtttcagt
    10861 gaaatcacaa tcaatcatgt attttcactg aaatatatta tattaaaaag aaacctaaaa
    10921 acattacaaa taacaatacc aaatttgaac cgataagata aaatgtcttg tcggttttta
    10981 atataacaag tgatattttg gaaataccaa aaatatatag attgtttata taaaatgatt
    11041 taaaatgttt tataacaaca tattttatgc aacacataaa atgggtaact aacatcacga
    11101 aatcattgta tttgttgtta cactgatgaa gcgatgtcac aaagaaatgt cgttatcaaa
    11161 cgaagcccga aatacatact gatactcgaa ataatgtaac gataaataac aatgataacg
    11221 attgattact acgcaaaaat atatgtaaat tgacgattta aatgcaattc gtatattttg
    11281 ccaatgattt acacattaaa atatttgaaa attgtcacaa ttcagtctaa tttaaaagga
    11341 agttaattac aaaattcaaa aatcatgcat cgtgtcattt agatattgaa aaagatgagt
    11401 caaatttgtt tcatatattt tccaataaca
//
LOCUS       DAHHSC010000066        11325 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.066, whole genome shotgun sequence.
ACCESSION   DAHHSC010000066 DAHHSC010000000
VERSION     DAHHSC010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 11325)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11325)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11325
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.066"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            85..1161
                     /locus_tag="NBI57_001337"
     CDS             85..1161
                     /locus_tag="NBI57_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide synthase oxygenase"
                     /protein_id="HCD1822291.1"
                     /translation="MLFKEAQAFIENMYKECHYETQIINKRLHDIELEIKETGTYTHT
                     EEELIYGAKMAWRNSNRCIGRLFWDSLNVIDARDVTDEASFLSSITYHITQATNEGKL
                     KPYITIYAPKDGPKIFNNQLIRYAGYDNCGDPAEKEVTRLANHLGWKGKGTNFDVLPL
                     IYQLPNESVKFYEYPTSLIKEVPIEHNHYPKLRKLNLKWYAVPIISNMDLKIGGIVYP
                     TAPFNGWYMVTEIGVRNFIDDYRYNLLEKVADAFEFDTLKNNSFNKDRALVELNYAVY
                     HSFKKEGVSIVDHLTAAKQFELFERNEAQQGRQVTGKWSWLAPPLSPTLTSNYHHGYD
                     NTVKDPNFFYKKKESNANQCPFHH"
     gene            1181..1975
                     /locus_tag="NBI57_001338"
     CDS             1181..1975
                     /locus_tag="NBI57_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HCD1822292.1"
                     /translation="MQLYYLGPKGTFSYLACRQYFSENEATFQPKSNLFEVIKAVADD
                     DTSIGVVPIENSIEGTINIVADALAQQDVFAHGEIRLDINFALYGNGTDSISDIKKVY
                     SIAPAISQTTNYIHQHQFDYDYVDSTIQSLTKIENGVAAIAPLGSGEAYGFTPIDTHI
                     EDYPHNVTRFLVIKNQQQFDQNATSLMFLITPMHDKPGLLASVLNTFALFNINLSWIE
                     SRPLKTQLGMYRFFVQADSAITTDIKKVIAILETLDFKVEMIGAFN"
     gene            2047..2154
                     /locus_tag="NBI57_001339"
     CDS             2047..2154
                     /locus_tag="NBI57_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1822293.1"
                     /translation="MVDFLLFVLFLHDAAVYVSLPLVQAIANVTFYFSF"
     gene            complement(2165..3727)
                     /locus_tag="NBI57_001340"
     CDS             complement(2165..3727)
                     /locus_tag="NBI57_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HCD1822294.1"
                     /translation="MAYFNQHQSMISKRYLTFFSKSKKKKPFSAGQLIGLILGPLLFL
                     LTLLFFHPQDLPWKGVYVLAITLWIATWWITEAIPIAATSLLPIVLLPLGHILTPEQV
                     SSEYGNDIIFLFLGGFILAIAMERWNLHTRVALTIINLIGASTSKILLGFMVATGFLS
                     MFVSNTAAVMIMIPIGLAIIKEAHDLQEANTNQTSIQKFEKSLVLAIGYAGTIGGLGT
                     LIGTPPLIILKGQYMQHFGHEISFAKWMIVGIPTVIVLLGITWLYLRYVAFRHDLKYL
                     PGGQTLIKQKLDELGKMKYEEKVVQTIFVLASLLWITREFLLKKWEVTSSVADGTIAI
                     FISILLFIIPAKNTEKHRRIIDWEVAKELPWGVLILFGGGLALAKGISESGLAKWLGE
                     QLKSLNGVSPILIVIVITIFVLFLTEVTSNTATATMILPILATLSVAVGVHPLLLMAP
                     AAMAANCAYMLPVGTPPNAIIFGSGKISIKQMASVGFWVNLISAIIIILVVYYVMPIV
                     LGIDINQPLPLK"
     gene            complement(3932..5029)
                     /locus_tag="NBI57_001341"
     CDS             complement(3932..5029)
                     /locus_tag="NBI57_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pectate lyase"
                     /protein_id="HCD1822295.1"
                     /translation="MYINMKDYGLTGINKTKDTRAIQRALNRGRCKPTTVYIPKGTYD
                     ICKPLTIYGNTTLLLDNETILRRCHSGPLLKNGHRFGFYRGYNGHSHIHIKGGKFDMN
                     GVSYPYNNTAMCIGHAEDIQLIGVTIKNVVSGHAIDACGINGLYIKSCSFEGFIDYSG
                     ERFYSEAIQLDIQVPGAFPKFGTTDGTITKNVVIEDCYFGPSELPEMGSWNRAIGSHA
                     SRHNRYYENIHIRNNIFEDIQGYALTPLKYKDAFIINNKFINCEGGIRYLGVRDGKNA
                     ADVMTGKDLGSQAGINMNIIGNEFKGSMSKDAIHVRNYNNVKHKDVLIVGNTFNNSTQ
                     SIHLEDIDTVFLSPVEAGIQVTTINIDEIKK"
     gene            5398..5958
                     /locus_tag="NBI57_001342"
     CDS             5398..5958
                     /locus_tag="NBI57_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HCD1822296.1"
                     /translation="MTHRALLVVDYSYDFIADDGLLTCGKPGQNIEDFIVSRINDFNY
                     YQDHIFFLMDLHYLHDIHHPESKLFPPHNIVDTSGRELYGKVGKLYETIKAQPNVHFI
                     DKTRYDSFFGTPLDSLLRERSINQVEIVGVCTDICVLHTAISAYNLGYKISVPAEGVA
                     SFNQKGHEWALAHFKNSLGAEVEQHV"
     gene            6011..6940
                     /locus_tag="NBI57_001343"
     CDS             6011..6940
                     /locus_tag="NBI57_001343"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HCD1822297.1"
                     /translation="MAKTYIFGHKNPDTDAISSAIIMAEFEQLRGNSGAKAYRLGDVS
                     AETQFALDTFNVPAPELLTDDLDGQDVILVDHNEFQQSSDTIASATIKHVIDHHRIAN
                     FETAGPLCYRAEPVGCTATILYKMFRERGFEIKPEIAGLMLSAIISDSLLFKSPTCTQ
                     QDVKAAEELKDIAKVDIQKYGLDMLKAGASTTDKSVEFLLNMDAKSFTMGDYVTRIAQ
                     VNAVDLDEVLNRKEDLEKEMLAVSAQEKYDLFVLVVTDIINSDSKILVVGAEKDKVGE
                     AFNVQLEDDMAFLSGVVSRKKQIVPQITEALTK"
     gene            complement(7133..7306)
                     /locus_tag="NBI57_001344"
     CDS             complement(7133..7306)
                     /locus_tag="NBI57_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1822298.1"
                     /translation="MIVVAVNFKWSFELYMLKVENRAMNRVHNNIYNFNHNEKGRKKT
                     KIYTQHRKYFKKM"
     gene            7358..8737
                     /locus_tag="NBI57_001345"
     CDS             7358..8737
                     /locus_tag="NBI57_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HCD1822299.1"
                     /translation="MNIIEQKFYDSKAFFNTQQTKDISFRKDQLKKLSKAIKSYESDI
                     LEALYTDLGKNKVEAYATEIGITLKSIKNARKELKNWTKTKNVDTPLYLFPTKSYIKK
                     EPYGTVLIIAPFNYPFQLVFEPLIGAIAAGNTAIIKPSELTPNVARVIKRLINETFDA
                     NYIEVIEGGIEETQTLIHLPFDYVFFTGSENVGKIVYQAASENLVPVTLEMGGKSPVI
                     VDETANIKVASERICFGKFTNAGQTCVAPDYILVHESVKDDLITALSKTLREFYGQNI
                     QQSPDYGRIVNLKHYHRLTSLLNSAQMNIVFGGHSDEDERYIEPTLLDHVTSDSAIMQ
                     EEIFGPILPILTYQSLDEAIAFIHQRPKPLSLYLFSEDENATQRVINELSFGGGAIND
                     TLMHLANPKLPFGGVGASGMGRYHGKYSFDTFTHEKSYIFKSTRLESGVHLPPYKGKF
                     KYIKAFFKN"
     gene            complement(8857..9885)
                     /locus_tag="NBI57_001346"
     CDS             complement(8857..9885)
                     /locus_tag="NBI57_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HCD1822300.1"
                     /translation="MTNGYIGSYTKKNGKGIYRFELNENQSRIDLLEIGFELEASTYL
                     VRNNEVLYGINKEGEQCGVASLKIDDNGELHLLNKCLSSKAGTGCYVSISEDKRYLFE
                     AVYGAGIIRMYELNTHTGEIIRLIQELAHDFPTGTHERQDHPHAHYINQTPDGKYVAV
                     TDLGADRIVTYKFDDNGFEFYKESLFKDSDGTRHIEFHDNGKFAYVVHELSNTVSVAE
                     YNDGKFEELERHLTIPENFDGDTKLAAVRLSHDQQFLYVSNRGHDSIAIFKVLDNGQH
                     LELVTITESGGQFPRDFNIASSDDLLVCAHEQGDSVVTVFERSKETGKITLCDNTRVA
                     SEGVCVIF"
     gene            10155..10556
                     /locus_tag="NBI57_001347"
     CDS             10155..10556
                     /locus_tag="NBI57_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YolD-like family protein"
                     /protein_id="HCD1822301.1"
                     /translation="MTSYEEETDYRNVPRELLDNNIPMGRGMIKWAPFATLPEQFETI
                     QQYIIDQNKITRPVLSDDQLAELNIRLHEALQYAQPVEVKFYNNGFVDSVRLTIYRID
                     AINYEIDGYVYNQQQRQKISIFDILDIVLLP"
     gene            complement(10574..10782)
                     /locus_tag="NBI57_001348"
                     /pseudo
     CDS             complement(10574..10782)
                     /locus_tag="NBI57_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000277741.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(11129..11302)
                     /locus_tag="NBI57_001349"
     CDS             complement(11129..11302)
                     /locus_tag="NBI57_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1822302.1"
                     /translation="MWNFIKCVFKFVFSLVAITTLVAGVGVVAFAYIFKKDFEDIERK
                     TKEIISDIESKNN"
BASE COUNT         3848 a         1944 c         1719 g         3814 t
ORIGIN      
        1 atcaaccaac aatttaatga catatacata atttttaaga gtattttaat aatgtagact
       61 ataatataaa gcgaggtgtt gttaatgtta tttaaagagg ctcaagcttt catagaaaac
      121 atgtataaag agtgtcatta tgaaacgcaa attatcaata aacgtttaca tgacattgaa
      181 ctagaaataa aagaaactgg gacatataca catacagaag aagaacttat ttatggtgct
      241 aaaatggctt ggcgtaattc aaatcgttgc attggtcgtt tattttggga ttcgttaaat
      301 gtcattgatg caagagatgt tactgacgaa gcatcgttct tatcatcaat tacttatcat
      361 attacacagg ctacaaatga aggtaaatta aagccgtata ttactatata tgctccaaag
      421 gatggaccta aaattttcaa caatcaatta attcgctatg ctggctatga caattgtggt
      481 gatcctgctg aaaaagaagt tacacgctta gcaaatcact taggttggaa aggaaaaggt
      541 actaattttg acgtgttacc actgatttac caattaccta atgagtcagt taaattttac
      601 gaatatccta cttcattaat taaagaagta cctattgaac ataatcatta tccaaaatta
      661 agaaaattga acttaaaatg gtatgcagtc cctatcattt ccaatatgga cttaaaaatc
      721 ggtggcattg tatatccaac tgcacccttt aacggttggt atatggtaac tgaaattggc
      781 gtacgtaact ttattgatga ttaccgttac aatttactag aaaaagttgc agatgcgttt
      841 gaatttgata cacttaaaaa taattcattt aataaagatc gagcacttgt tgaattgaac
      901 tatgctgtgt atcattcctt taaaaaagaa ggcgtatcaa ttgtcgatca tttgaccgct
      961 gcaaagcaat tcgaactatt cgaacgtaac gaagcacaac aaggtcgtca agttaccgga
     1021 aaatggtctt ggctagcacc gccattatct ccaacattga cgtcaaatta tcatcacgga
     1081 tatgacaata cagtaaaaga tccaaacttt ttctataaaa agaaagaatc aaatgctaac
     1141 cagtgccctt tccatcatta agttagtaga ggtgtagcat atgcaattat attatttagg
     1201 ccctaaaggt acattttcat atttggcttg tcgtcagtat ttttctgaaa atgaagcaac
     1261 ttttcaacca aagtcaaatt tatttgaagt catcaaggca gttgcagatg atgacacatc
     1321 gattggtgtt gtccctattg aaaattctat agaaggaaca atcaacattg tggcagatgc
     1381 acttgcacag caagatgtct ttgcacacgg tgaaatacgt ttagatatta actttgcatt
     1441 atatggtaat ggaacagatt ccatttctga tattaaaaaa gtgtattcta ttgcaccagc
     1501 aatcagtcaa acaacaaatt acatacatca acatcaattt gactatgatt atgtcgacag
     1561 tacaattcaa agtttgacta aaattgaaaa tggcgttgca gcaattgcac ctttaggtag
     1621 tggcgaagct tatggtttta cacctattga tacacatatt gaagactatc cacataacgt
     1681 gacacgtttt ttagttataa aaaatcagca gcaatttgat caaaatgcga catcattgat
     1741 gtttttaatt acaccaatgc atgataaacc tgggctgtta gcaagtgtgc ttaatacatt
     1801 tgctttgttt aatattaatt tatcctggat tgagtctcgt cctttaaaaa cacaattagg
     1861 tatgtatcga tttttcgttc aggctgattc tgcaattaca accgatataa agaaagtcat
     1921 tgctatttta gaaaccttag atttcaaagt agaaatgatt ggtgctttta attaaacttt
     1981 attttctagg ttctacaatt taaataaaat aaagagtcaa ccaaggatta aaataggaat
     2041 ccatccttgg ttgactttct tttgtttgta ttgtttttac acgatgctgc ggtatacgtt
     2101 tcattaccac tcgttcaagc aattgctaat gtaacctttt atttttcgtt ctaatctgca
     2161 attactattt caatggcagt ggttgattta tatcaatacc taaaactata ggcattacat
     2221 aatacacgac taaaataata attattgcac tgattaagtt tacccagaat cctactgatg
     2281 ccatttgttt gatagatatt ttacctgaac caaagataat tgcattcggt ggtgtcccta
     2341 ctggtaacat gtatgcacag ttagccgcca tagctgcagg tgccataagt agtaatggat
     2401 gcactccaac agcaacagac aacgttgcta aaatcggtaa aatcatcgtt gcagttgcag
     2461 tattagatgt cacttcagtt aaaaataaga caaagattgt tatgacaatt acaataagaa
     2521 tcggactaac accatttaat gatttcaact gttcgcctaa ccattttgct aaaccacttt
     2581 cagaaatacc tttcgctaat gctaaaccgc caccaaataa aattaataca ccccaaggga
     2641 gctcttttgc aacttcccag tcaatgatac ggcgatgttt ttcagtattt ttagctggaa
     2701 taataaataa taatattgat ataaaaatag caatcgtacc atctgcaaca gatgacgtaa
     2761 cttcccattt tttcagaaga aactctcttg taatccataa taagctggca agtacaaaga
     2821 tagtttgtac taccttttct tcatacttca ttttgccaag ctcgtctaac ttttgtttaa
     2881 ttaacgtctg accaccaggt aaatatttca aatcatgtct aaacgcaaca tatcttaaat
     2941 agagccaagt aatacctaac aaaacaatga ccgttggaat ccctacaatc atccatttag
     3001 caaaactaat ttcatgtcca aaatgttgca tgtattgtcc ttttaaaata attaatggcg
     3061 gggttccgat taatgtaccc aagccaccaa tcgtacctgc atagccaatt gctaaaacta
     3121 gagatttttc aaacttttga atacttgttt gattcgtatt ggcttcttgt aaatcatgtg
     3181 cttccttaat aattgctaaa ccaatcggaa tcataatcat tacagctgca gtgttcgata
     3241 caaacataga taagaatcct gttgccacca tgaatccaag taatattttt gaagtgcttg
     3301 caccaattaa attaataata gtcaaagcta ctctcgtatg taaattccat ctttccattg
     3361 caattgccaa aataaatcca cctaaaaaca aaaagataat atcattgcca tattcggatg
     3421 atacttgttc tggtgtaagt atgtgaccta atggtaataa cacaattggt aataagctcg
     3481 ttgctgcaat aggaattgct tcagtaatcc accaagtcgc aatccataaa gtaatcgcta
     3541 agacatatac gcctttccaa ggtaagtctt gtggatgaaa gaacaataat gttaatagga
     3601 aaagtaatgg acctaatatt agtccaataa gttgtcccgc actaaacggt ttctttttct
     3661 ttgattttga aaagaatgtt aaataccttt tcgatatcat tgattgatgt tgattgaaat
     3721 aagccataaa tttccccctc atgatgatta tgacaagcta cttcaactca ctatattaga
     3781 gtatagaaag ttgtctatct tgtattatca ttaccgtcaa aatacccctt ttatcagata
     3841 attgggttta ttatagcata tttataccgt tttatgaatg cgctttcaaa aattatctac
     3901 tattcttaat cctaatgtca tgcgaaactt tttacttttt tatttcatct atattgattg
     3961 tagtaacttg aatacccgct tcaacaggac ttaaaaacac tgtatcaata tcttctaaat
     4021 gaattgattg agtcgaatta ttgaatgtat tcccaacgat taatacatct ttatgtttaa
     4081 cattattata attacgtaca tgtatcgcat ctttagacat tgatccttta aattcatttc
     4141 caattatatt catatttatg cctgcttggg aacctaagtc ttttcctgtc atcacatctg
     4201 ctgcattttt accatctcta actcctaaat atctaatacc accctcacag ttaataaact
     4261 tattattaat aatgaaagca tctttatact tcaagggagt taatgcataa ccttgtatat
     4321 cttcaaatat attatttcta atatgaatat tctcatagta acgattatgt ctacttgcat
     4381 gtgagccaat agcacgattc caacttccca tttcgggcaa ttctgaaggt ccaaaataac
     4441 aatcttcgat aacgacattt ttcgttatcg taccatctgt cgttccgaat tttggaaaag
     4501 caccaggtac ttgaatgtct aattgtattg cttcagaata aaagcgttcg ccactatagt
     4561 ctatgaatcc ttcaaatgaa cagcttttaa tatagagtcc gttaatccca caagcatcaa
     4621 ttgcatgacc actcactaca ttcttaatgg tcacacctat taattgaata tcttcagcat
     4681 gcccaatgca catagctgta ttgttataag gatacgatac accattcata tcaaacttgc
     4741 cgcctttaat atgaatatga ctgtgtccat tataaccacg ataaaaacca aagcgatgac
     4801 cattttttaa taaaggacca gaatgacatc ggcgtaaaat agtttcatta tctaacaaaa
     4861 gtgttgtatt gccatatatc gttaatggtt tgcaaatatc atacgtccct ttcggtatat
     4921 aaactgtcgt tggtttacat cttccacgat ttaacgcacg ttgtattgct cgagtatctt
     4981 tagttttgtt tatgcctgtt aacccataat ctttcatatt gatatacata aatcgtcatc
     5041 ctttgtctat tttctaatta ctcatatatt aaccaattat tcaaattata atcgtgttag
     5101 gttgatttag cagatagtca taaatctcgt gatgccatct attagcattt ttatattcaa
     5161 aacatcaaca cttgatatta gttctaagtt tgtcacattt agtattctat tacagctatt
     5221 ttaaaactat cgcaaaaact tcttatatta tttgttgatt gcgtgatttg ctcttccaaa
     5281 aaagttgatt aatctatata cttaatctat atttatatat taaccattag ggttaaaaat
     5341 tactctagca tttatgaata gatgggagtt tattttatta tataggagag atgttgaatg
     5401 acacatcgcg cactattagt tgttgactat tcatatgact ttatcgcaga tgacggctta
     5461 ctaacatgcg gtaaacctgg acaaaatatt gaagatttta ttgtttctcg tatcaatgac
     5521 tttaattatt atcaagacca tatattcttt ttaatggatt tacattattt acatgacatt
     5581 catcatcctg aaagtaaatt attcccacca cacaatatcg tagatacaag tggtagagaa
     5641 ttatacggta aagtaggtaa attatacgaa acaattaaag cgcaacctaa tgtacatttc
     5701 attgataaaa cgcgctatga ttcgttcttt ggtaccccgc ttgatagttt attgagagaa
     5761 agaagtatta atcaagtcga aatcgttggt gtatgtaccg atatttgcgt gttacataca
     5821 gcaatttctg catacaactt aggttataaa atttcagtac ctgctgaggg agtggcttca
     5881 tttaatcaaa aagggcatga atgggcactt gcacatttca aaaactcatt aggtgcagag
     5941 gtagaacaac acgtttaaat cgtgctaaaa taattataaa gaatacaatt tacaagggag
     6001 atatttgaca atggctaaaa catatatttt cggacataag aatccagaca ctgatgcaat
     6061 ttcatctgcg attattatgg cagaatttga acaacttaga ggtaattcag gagccaaagc
     6121 ataccgttta ggtgatgtga gtgcagaaac tcaattcgcg ttagatacat ttaatgtacc
     6181 tgctccggaa ttattaacgg atgatttaga tggtcaagat gttatcttag ttgatcataa
     6241 cgaattccaa caaagttctg atacgattgc ctctgctaca attaagcatg taattgatca
     6301 tcacagaatt gcaaatttcg aaactgctgg ccctttatgt tatcgtgctg aaccagttgg
     6361 ttgtacagct acaattttat acaaaatgtt tagagaacgt ggctttgaaa ttaaacctga
     6421 aattgccggt ttaatgttat cagcaattat ctcagatagc ttacttttca aatcaccaac
     6481 atgtacacaa caagacgtta aagcagctga agaattaaaa gatattgcta aagttgatat
     6541 tcaaaagtac ggcttagata tgttaaaagc aggtgcttca acaactgata aatcagttga
     6601 attcttatta aacatggatg ctaaatcatt tactatgggt gactatgtga ctcgtattgc
     6661 acaagttaat gctgttgacc ttgacgaagt gttaaatcgt aaagaagatt tagaaaaaga
     6721 aatgttagct gtaagtgcac aagaaaaata tgacttattt gtacttgttg ttactgacat
     6781 cattaatagt gattctaaaa ttttagttgt aggtgctgaa aaagataaag tcggcgaagc
     6841 attcaatgtt caattagaag atgacatggc cttcttatct ggtgttgttt ctcgaaaaaa
     6901 acaaatcgta cctcaaatca ctgaagcatt aacaaaataa tactatatta ctgtctaatc
     6961 atagacatgt tgtatttaac taacagttca ttaaagtaga atttatttca ctttcaatga
     7021 actgtttttt atttacgttt gactaattta caaccttttc aatagtagtt ttcattcctt
     7081 tagctatcct aacccacaga ttagtgattt ctatacaatt cctcttttgt ctttacattt
     7141 tcttaaaata tttgcgatgt tgagtataaa tttttgtttt cttcctacct ttttcgttat
     7201 gattaaagtt ataaatatta ttatgtacac gattcatcgc tctattttca actttcaaca
     7261 tatataattc gaaagaccat ttaaaattaa cggccacaac aatcaaatca attaatcact
     7321 ttttccaaaa taatcatata aggaggttct tttcattatg aatatcattg agcaaaaatt
     7381 ttatgacagt aaagcttttt tcaatacaca acaaactaaa gatattagtt ttagaaaaga
     7441 tcaattaaag aagttaagca aagctattaa atcatacgag agcgatattt tagaagcact
     7501 atatacagat ttaggaaaaa ataaagtcga agcttatgct actgaaattg gcataacttt
     7561 gaaaagtatc aaaaatgccc gtaaggaact taaaaactgg actaaaacaa aaaatgtaga
     7621 cacaccttta tatttatttc caacaaaaag ctatatcaaa aaagaacctt atggaacagt
     7681 tttgatcatt gcaccattta actatccttt tcaactagta ttcgaacctt taatcggtgc
     7741 tattgcagca ggtaatacag caattattaa accatctgag ttgacaccaa atgttgcacg
     7801 agtgattaaa cgattaatca atgaaacatt tgatgcaaat tacattgaag ttattgaggg
     7861 aggaattgaa gaaacgcaaa cgttaattca cttacctttt gactatgtct tctttacagg
     7921 aagtgaaaat gtaggcaaaa tcgtttatca agctgccagc gaaaatttag ttcctgtgac
     7981 attagaaatg ggtggtaaat ctccagtcat cgttgatgaa acagcgaata ttaaagttgc
     8041 tagtgagcgc atttgttttg ggaaattcac taatgccggc caaacatgtg ttgcaccaga
     8101 ttacatttta gtacacgaat ctgtaaaaga tgatttaatc acagccctat caaaaacgtt
     8161 gcgtgaattt tatggtcaaa atatacaaca aagtccagat tatggccgca ttgtaaacct
     8221 taaacattat catcgtctga cttcattact taacagtgca caaatgaata ttgtatttgg
     8281 tggtcatagt gatgaggatg aacgttatat agaaccaaca ttgttagatc acgttacaag
     8341 tgattcagca attatgcaag aagaaatttt tggtcctatc ttaccgattt taacgtatca
     8401 gtcattggat gaagcaatag cctttattca ccaaagacca aaacctttga gtttatattt
     8461 atttagcgaa gatgaaaatg ctacacaacg tgtaataaac gagttatcat ttggcggcgg
     8521 cgctattaat gatacattga tgcacctagc gaatcctaaa ttaccatttg gtggtgttgg
     8581 cgcctcaggt atgggacgct atcatggtaa atattcattc gacactttta cacatgaaaa
     8641 aagctacatt ttcaaatcta cacgattaga atcaggtgtc catttaccac catataaagg
     8701 taaatttaaa tacatcaaag ctttctttaa aaattaattt ttgagacaaa aataaaccat
     8761 gcttccataa tttgatgttc ttgaaaacaa taaatttcaa ggtatacaca aattttttgt
     8821 aaacatggtt ttctttttat catgattaat taaagattaa aatatgacac atacaccttc
     8881 agatgctaca cgagtattat cacatagagt aattttacct gtttctttac ttctttcaaa
     8941 aacagttaca actgaatctc cttgctcatg agcacaaact aaaaggtcat ctgatgaggc
     9001 aatattaaaa tctcttggga attgaccacc actttcagta attgttacta gttctaagtg
     9061 ttgaccatta tcaagaactt taaaaattgc aatgctatca tgccctctat tagatacata
     9121 taagaattgt tgatcatgag acaaacgcac tgctgcaagt ttagtatctc catcaaagtt
     9181 ttcaggaatt gttaaatgac gctcgagctc ttcaaattta ccgtcattat attctgcaac
     9241 actcacagta tttgataatt cgtgtacgac ataagcaaat tttccattat catgaaattc
     9301 aatatgtctt gtcccatcac tatctttaaa taaagattct ttataaaatt caaacccgtt
     9361 gtcatcaaat ttataagtaa cgatacgatc agcacctaaa tctgttactg caacgtactt
     9421 accatctgga gtttgattaa tataatgtgc gtgtggatga tcttgtcttt catgtgtacc
     9481 tgttggaaaa tcatgtgcaa gttcttgaat tagacgtata atttcacctg tgtgcgtatt
     9541 taattcatac atacgtatga tgccagcacc atataccgct tcaaataaat atcgtttatc
     9601 ttctgaaatc gatacataac aacctgtacc agcttttgaa gacaaacatt tatttaataa
     9661 atgcaattca ccattgtcat caattttcaa actcgcaaca ccgcattgtt ctccttcttt
     9721 gttgattcca tataaaactt cgttattacg caccaaatat gtagacgctt ctaattcaaa
     9781 tcctatttct aataaatcaa tacgtgactg attttcgttt aattcaaaac gataaatccc
     9841 tttaccattc tttttagtgt aagaaccaat atatccattt gtcattactt ttacccctcc
     9901 atgtcagtct tattttcatt ttattaaatt ttagctagaa ttaacaatca tttatcattt
     9961 ccttaacgct aaaactgttt aactacttta aaagaactat ttgctgtgat atgtcattat
    10021 tcgattttaa ttaatccctc ttttttactt acacgagcat taattattaa cgcgtttatg
    10081 cttccacaaa atttttgttg caaaacagaa cacatgttcg tataatgtaa ttatcagatt
    10141 ggggtgaatt aaggatgaca agttatgaag aagaaactga ttatcgtaac gttccaagag
    10201 aattactaga taacaatata cctatgggaa gaggcatgat taaatgggct ccttttgcaa
    10261 cattacccga acaattcgaa acgattcaac aatacattat cgaccaaaac aaaattacac
    10321 gtcctgtatt atcagatgat caattggctg agctaaacat tcggctacac gaggcgctac
    10381 aatatgccca acctgtagaa gttaaatttt ataataatgg ctttgtagat tcagtacgct
    10441 taaccattta tcgtattgat gctattaatt atgaaattga tggttatgtt tacaatcagc
    10501 aacaacgtca aaaaatttct atctttgata tcttagatat tgtcttgtta ccttaatgct
    10561 ttgaacataa ttaaacagat tgagtatatg catataagat gatttttaac atcatttttg
    10621 gatgatagga tgttgcgcca cgatgatgtc tgaattcatc gaattcgcta tcaggtattg
    10681 tttcaacaat ttcattaaca tatcgtgaaa tatcattttg agggattcta acagaagttt
    10741 ctattggtag tgtagttggg tcatgttata atttttatac ataaggcacc tcgttaattt
    10801 agtttagtgg tatttattaa attataagaa gggacccaac acagaaaatt cattttattg
    10861 aattttacat ttatgtgcaa gttgggcaaa gtgtcttatt ttttaaaagt atttcaaagt
    10921 aaaattacat gttaatacgt agtattaatg gcgagactcc tgagggagca gtgccagtcg
    10981 aagaccaagg ctgagacggc accctaggaa agcgaagcca ttcaatacga agtattgtat
    11041 aaatagagaa cagcagtaag atattttcta attgaaaatt atcttactgc tgttttttag
    11101 ggatttatgt cccagcctct aaatgttatt agttattttt actttcaata tcagaaataa
    11161 tttctttagt ttttctttca atatcttcaa aatctttttt aaagatataa gcaaatgcta
    11221 ctacaccaac accagcaact aatgttgtaa tagcaactaa gctaaatacg aatttaaaca
    11281 cacatttaat aaaattccac atctcaattt cacctctgtt aaatt
//
LOCUS       DAHHSC010000067        11287 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.067, whole genome shotgun sequence.
ACCESSION   DAHHSC010000067 DAHHSC010000000
VERSION     DAHHSC010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 11287)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11287)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11287
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.067"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            118..900
                     /gene="modA"
                     /locus_tag="NBI57_001350"
     CDS             118..900
                     /gene="modA"
                     /locus_tag="NBI57_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HCD1822303.1"
                     /translation="MKMKRFIAIVMALFLVLAGCSNSNDNNESKKDDADNGKKQEIQV
                     AAAASLTDVTKKLASEFKKEHKNADIKFNYGGSGALRKQIESGAPVDVFMSANTKDVD
                     ALKDKNKAHDTYKYAKNSLVLIGDKDSNYTSVKDLKDNDKLALGEVKTVPAGKYAKQY
                     LDNNNLFKEVESKIVYAKDVKQVLNYVEKGNAKQGFVYKTDLYKQNKKIDTVKVIKEV
                     ELKKPITYEAGATSDSKLAKEWMEFLKSDKAKEILKEYHFAA"
     gene            914..1585
                     /gene="modB"
                     /locus_tag="NBI57_001351"
     CDS             914..1585
                     /gene="modB"
                     /locus_tag="NBI57_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HCD1822304.1"
                     /translation="MPDLTPFWISIRVAVISTIIVTVLGIFISKWLYRRKGSWVKVLE
                     SLLILPIVLPPTVLGFILLIIFSPRGPIGQFFANVLHLPVVFTLTGAVIASVIVSFPL
                     MYQHTVQGFRGIDTKMINTARTMGASETKIFLKLILPLAKRSILAGIMMSFARALGEF
                     GATLMVAGYIPNKTNTLPLEIYFLVEQGRENEAWLWVLVLVAFSIVVISTINLLNKDK
                     YKEVD"
     gene            1586..2191
                     /locus_tag="NBI57_001352"
     CDS             1586..2191
                     /locus_tag="NBI57_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCD1822305.1"
                     /translation="MLKINVKYQLKNTLIRINIDDTEPKIYAVRGPSGIGKTTVLNMI
                     AGLRKADEAIIEVNGQLLTDTTKNVNVKIQQRRIGYLFQDYQLFPNMTVYKNITFMAE
                     PSKHIDQLIQTLNINHLMKQYPMTLSGGEAQRVALARALSTKPDLILLDEPFSSLDDT
                     TKDESITLVKRIFNEWQIPIIFVTHSNYEAEQMAHEIITIG"
     gene            2361..3365
                     /locus_tag="NBI57_001353"
     CDS             2361..3365
                     /locus_tag="NBI57_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HCD1822306.1"
                     /translation="MSQERYSRQILFKQIGEIGQSKINQKCALIIGMGALGTHVAEGL
                     VRAGIAKLIIVDRDYIEFSNLQRQTLFTEEDALKMMPKVVAAKKHLLALRSDVDIDGC
                     IAHVDYYFLETHGQDVDVIIDATDNFETRQLINDFAYKHRIPWIYGGVVQSTYTEAAF
                     IPGKTPCFNCLVPQLPALNLTCDTVGVIQPAVTMATSLQLRDAMKVLTEQPIDTKITY
                     GDIWEGSHYSFGFSKMQRSDCTTCGDVPSYPYLNKNEQRYATLCGRDTVQYENASITH
                     DILVQFLKQHQLNYRSNSYMVMFEFKGHRIVAFKGGRFLIHGMTRTSDATHLMNLLFG
                     "
     gene            3396..3902
                     /locus_tag="NBI57_001354"
     CDS             3396..3902
                     /locus_tag="NBI57_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein MoaB"
                     /protein_id="HCD1822307.1"
                     /translation="MGEHQNVKLNRTVKAAVLTVSDTRDFVTDKGGQCVRQLLQADDV
                     EVSDAHYTIVKDEKVAITTQVKKWLEEDIDVIITTGGTGIAQRDVTIEAVKPLLTKEI
                     EGFGELFRYLSYVEDVGTRALLSRAVAGTVNNKLIFSIPGSTGAVKLALEKLIKPELN
                     HLIHELTK"
     gene            complement(3904..4398)
                     /gene="moaC"
                     /locus_tag="NBI57_001355"
     CDS             complement(3904..4398)
                     /gene="moaC"
                     /locus_tag="NBI57_001355"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HCD1822308.1"
                     /translation="MTEFTHINQQGHAKMVDVSDKQITKRTAVAHSSITVNETIFKQI
                     SNNTNTKGNVLNTAQIAGIMAAKNTSTIIPMCHPLPLTGIDVHFSWDETNAPLYTLNI
                     QTTVSTTGKTGVEMEALTAASATALTIYDMTKAVDKGMIIGETYLESKSGGKSGDFQR
                     QSGQ"
     gene            4461..5720
                     /locus_tag="NBI57_001356"
     CDS             4461..5720
                     /locus_tag="NBI57_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HCD1822309.1"
                     /translation="MVVEKRNPIPVKEAIQRIVNQQSSMPAITVALEKSLNHILAEDI
                     VATYDIPRFDKSPYDGFAIRSVDSQGASGQNRIEFKVIDHIGAGSVSDKLVGDHEAVR
                     IMTGAQIPNGADAVVMFEQTIELEDTFTIRKPFSKNENISLKGEETKTGDVVLKKGQV
                     INPGAIAVLATYGYAEVKVIKQPSVAVIATGSELLDVNDVLEDGKIRNSNGPMIRALA
                     EKLGLEVGIYKTQKDDLDSGIQVVKEAMEKHDIVITTGGVSVGDFDYLPEIYKAVKAE
                     VLFNKVAMRPGSVTTVAFADGKYLFGLSGNPSACFTGFELFVKPAVKHMCGALEVFPQ
                     IIKATLMEDFTKANPFTRFIRAKATLTSAGATVVPSGFNKSGAVVAIAHANCMVMLPG
                     GSRGFKAGHTVDIILTESDAAEEELLL"
     gene            5717..6202
                     /gene="mobB"
                     /locus_tag="NBI57_001357"
     CDS             5717..6202
                     /gene="mobB"
                     /locus_tag="NBI57_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein B"
                     /protein_id="HCD1822310.1"
                     /translation="MILQIVGYKKSGKTTLMRHIVSFLKTHGYTVATIKHHGHAKEDI
                     QLQDSDVDHMKHFEAGADQSIVQGFQYQQTVTRVDNQNLTQIIEKSVTIDTNIVLVEG
                     FKNADFEKVVVYRDEEELQVLQQLSNVCYSINVREHEDFTAFEQWLLNKIKNDCDTQL
                     T"
     gene            6216..6662
                     /locus_tag="NBI57_001358"
     CDS             6216..6662
                     /locus_tag="NBI57_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein MoaE"
                     /protein_id="HCD1822311.1"
                     /translation="MKQFEIVIEPIQTEQYREFTINEYQGAVVVFTGHVREWTKGVKT
                     EYLEYEAYIPMAEKKLAQIGDEINEKWPGTITSIVHRIGPLQISDIAVLIAVSSPHRK
                     DAYRANEYAIERIKEIVPIWKKEIWEDGSKWQGHQKGNYEEAKREE"
     gene            6668..6901
                     /gene="moaD"
                     /locus_tag="NBI57_001359"
     CDS             6668..6901
                     /gene="moaD"
                     /locus_tag="NBI57_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin converting factor subunit 1"
                     /protein_id="HCD1822312.1"
                     /translation="MKVLYFAEIKDILQKAQEDIVLEQALTVQQFEDLLFERYPQINN
                     KKFQVAVNEEFVQKSDFIQPNDTVALIPPVSGG"
     gene            6908..7507
                     /gene="mobA"
                     /locus_tag="NBI57_001360"
     CDS             6908..7507
                     /gene="mobA"
                     /locus_tag="NBI57_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase MobA"
                     /protein_id="HCD1822313.1"
                     /translation="MKAIILAGGHSVRFGKPKAFAEVNGETFYSRVIKTLESTNMFNE
                     IIISTNAQLATQFKYPNVVIDDENHNDKGPLAGIYTIMKQHPEEELFFVVSVDTPMIT
                     GKAVSTLYQFLVSHLIENHLDVAAFKEDGRFIPTIAFYSPNALGAITKALHSDNYSFK
                     NIYHELSTDYLDVRDVDAPSYWYKNINYQHDLDALIQKL"
     gene            7527..8549
                     /gene="moaA"
                     /locus_tag="NBI57_001361"
     CDS             7527..8549
                     /gene="moaA"
                     /locus_tag="NBI57_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HCD1822314.1"
                     /translation="MVEQIKDKLGRPIRDLRLSVTDRCNFRCDYCMPKEVFGDDFVFL
                     PKNELLTFDEMARIAKVYAELGVKKIRITGGEPLMRRDLDVLIAKLNQIDGIEDIGLT
                     TNGLLLKKHGQKLYDAGLRRINVSLDAIDDTLFQSINNRNIKATTILEQIDYATSIGL
                     NVKVNVVIQKGINDDQIIPMLEYFKDKHIEIRFIEFMDVGNDNGWDFSKVVTKDEMLT
                     MIEQHFEIDPVEPKYFGEVAKYYRHKDNGVQFGLITSVSQSFCSTCTRARLSSDGKFY
                     GCLFATVDGFNVKAFIRSGVTDEELKEQFKALWQIRDDRYSDERTAQTVANRQRKKIN
                     MNYIGG"
     gene            complement(8815..9000)
                     /locus_tag="NBI57_001362"
     CDS             complement(8815..9000)
                     /locus_tag="NBI57_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001789125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1822315.1"
                     /translation="MFRFISFGQLVVYSTTFIPKKDILQLNNLECDINVKEPLKINYV
                     VIFGSGTEITFSQNLFR"
     gene            9126..9476
                     /gene="sarV"
                     /locus_tag="NBI57_001363"
     CDS             9126..9476
                     /gene="sarV"
                     /locus_tag="NBI57_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator SarV"
                     /protein_id="HCD1822316.1"
                     /translation="MSNKVQRFIEAERELSQLKHWLKTTHKISIEEFVVLFKVYEAEK
                     ISGKELRDTLHFEMLWDTSKIDVIIRKIYKKELISKLRSETDERQVFYFYSTSQKKLL
                     DKITKEIEVLSVTN"
     gene            complement(9578..10789)
                     /locus_tag="NBI57_001364"
     CDS             complement(9578..10789)
                     /locus_tag="NBI57_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCD1822317.1"
                     /translation="MTNQGSLNKSPIFTKSFTINFFVNFIVYLCMYLLLVVIAGYSKQ
                     AFDASDSLAGLVVGLFIVGSLIGRFATGKFVNQIGPKRLLFIGLIALIITQLLYFIDG
                     SLAFLIFVRLINGIATAVVTTATGTIAAYVTPVNRKSEGISLFSLSLVLGTAIGPFLG
                     MLLITKYAIDLLFIICVILGILGLIISLFIKVDFEVTNNKTETDVINKPRFSIHQFIA
                     KEAIPVAVIMLLIGVTYASILTYLQAFALERHLVTAASYFFICYAIASLITRPIAGRL
                     MDDKNENIIVYPAFIMLFLSFVCLISSYQSWLILLAGACLGLGYGNLSSAMQSIAIKV
                     SPPIKYGIATSTFYVGLDAGVGFGPSFLGLFTHMFSYSEIFGFMAALAIITMLVYFLI
                     HGRHVTRNAMN"
     gene            complement(10782..11222)
                     /locus_tag="NBI57_001365"
     CDS             complement(10782..11222)
                     /locus_tag="NBI57_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix DNA-binding protein"
                     /protein_id="HCD1822318.1"
                     /translation="MLSQEFFNSFITIYRPYLKLAEPILEKHNIYYGQWLILRDIAKH
                     QPTTLIEISHRRAIEKPTARKTLKALIENDLITVENSLEDKRQKFLTLTPKGHELYEI
                     VCLDVQKLQQAVVAKTNISQDQMQETINVMNQIHEILLKEAHND"
BASE COUNT         4025 a         1606 c         2191 g         3465 t
ORIGIN      
        1 cttatgcaaa tttgacaatt gtttgaatca atatataaac aggcaacggt tcttttcaaa
       61 tataatagta agtgtataat gaaaatgtaa atattattaa aaatgggggt tcactcaatg
      121 aaaatgaaac gttttatagc tattgtaatg gcattatttt tagtattagc tggttgctct
      181 aattctaacg ataataatga aagtaaaaaa gatgacgcag acaatggtaa gaaacaagag
      241 attcaagttg cagcggcagc aagtttaaca gatgtaacca agaaattagc ttcagaattt
      301 aaaaaagagc ataaaaatgc tgatattaaa tttaactatg gtggatcagg ggcattaaga
      361 aaacaaattg aatcaggcgc acctgttgac gtatttatgt ctgcaaatac taaagatgta
      421 gatgcattaa aagacaagaa taaagcgcat gatacatata aatatgcgaa aaatagtcta
      481 gtattaattg gtgataaaga ttcaaattac acttcagtaa aagacttaaa agacaatgat
      541 aaattagcat taggtgaagt gaaaactgta ccagcaggaa aatatgcgaa acagtattta
      601 gataacaata acttatttaa agaagtcgaa agtaaaatcg tttatgctaa agatgtaaaa
      661 caagtattaa attatgttga aaagggtaat gcgaaacaag gttttgtgta taaaactgac
      721 ttatataaac aaaacaaaaa aattgatact gtaaaagtaa ttaaagaagt agaacttaag
      781 aaaccaatca catacgaagc tggtgctaca tcagatagta aattagcaaa agagtggatg
      841 gaattcttaa aatcagataa agctaaagaa atattaaaag aataccactt tgcagcataa
      901 ggagttgtaa tccatgcctg acttaacacc tttttggata tcaatacgag ttgctgtaat
      961 cagtacgatt attgtaacgg ttttaggtat ttttatatct aaatggttgt atcgtcgtaa
     1021 gggttcgtgg gttaaagtat tggaaagttt attgatatta cctattgttt tgccgccaac
     1081 ggtattaggt tttattctat taatcatctt ctcgccaaga ggaccaatcg gtcaattctt
     1141 tgcgaatgta ctacatttac ctgtagtgtt cactttgaca ggtgctgtga tagcatctgt
     1201 cattgttagt tttccactaa tgtatcaaca tactgtgcaa ggcttcagag gtatagacac
     1261 gaaaatgatt aatacagcta gaacgatggg agcaagtgaa acgaaaattt tcctcaaatt
     1321 aattttacca ttagctaaac gctctatttt agcaggtata atgatgagtt ttgctcgtgc
     1381 attaggtgag tttggtgcta cattaatggt tgcaggatat attccaaata aaacgaatac
     1441 actaccttta gaaatatact tcttagtgga acaaggtaga gaaaatgaag cgtggttatg
     1501 ggtattagtg ctagtcgcat tctctattgt ggttatatct acaattaatt tattgaataa
     1561 agataaatat aaggaggtcg actagatgct taaaatcaat gtgaaatatc aattaaagaa
     1621 cactttaatt cgcatcaata tagatgatac tgaaccaaaa atttatgcag ttcgtggtcc
     1681 atctggcatt ggtaaaacta ctgttttaaa tatgattgcc ggattacgta aagcagatga
     1741 agctattatc gaagtgaatg ggcaattgct tactgatacg acaaaaaacg tgaatgttaa
     1801 aattcaacaa cgacgtattg gatatctgtt tcaagactac caattgtttc ctaatatgac
     1861 ggtttataaa aatattactt ttatggctga accatctaaa cacatcgatc aattaattca
     1921 aactttaaac attaatcatt tgatgaaaca atatcctatg acattgtcag gtggagaggc
     1981 acaacgtgta gcacttgcac gtgcacttag cacgaaacca gatttaattt tattagatga
     2041 acctttttct agtttggatg atactacaaa agatgagagt attacattag ttaaacgtat
     2101 tttcaacgaa tggcaaatac caatcatatt tgtgacacat tcaaactatg aagcagaaca
     2161 aatggctcat gaaattatta caattgggta atcatttatt tgccattaaa gagtttagaa
     2221 cgtatttaaa attgtagaag tgaatgcttc tatcagcatt ttaatgatgt tttaaactct
     2281 tttttagggg cagttttttt gagagacatt gacgcgcgtc atataatgaa agtaatgata
     2341 aaaagaaagg ataacttaat gtgagtcaag aacgttattc aaggcaaatt ttatttaaac
     2401 aaataggtga aataggtcaa agcaaaataa atcaaaaatg tgcgttgatt attggtatgg
     2461 gcgcattagg tacacatgtg gccgaaggac ttgttagagc aggcattgct aaactaatca
     2521 ttgttgatag agattatatt gaatttagta atttacaaag acaaacattg tttactgaag
     2581 aagatgcttt gaaaatgatg cctaaagtgg ttgcagctaa aaagcatttg ctagcgttac
     2641 gtagtgatgt tgatattgat ggttgtattg cccatgtgga ttattatttt ttggaaacac
     2701 atggacagga cgttgacgtt attattgatg caaccgataa ctttgaaaca cgacaactga
     2761 ttaatgattt tgcatataaa catcgtatac cttggattta tggtggtgtt gtacagagta
     2821 catatacaga agctgcattt atacctggta aaacaccttg ctttaactgt ttggtaccac
     2881 aattgccagc attaaattta acatgtgata cagtaggggt cattcaacct gccgtgacga
     2941 tggcaacaag tttacaatta agagatgcga tgaaagtatt aacggaacaa ccaattgaca
     3001 caaaaataac ttatggcgat atttgggaag gtagtcatta ttcatttggt ttcagtaaaa
     3061 tgcaacgttc agactgtaca acttgtggag atgtaccaag ttatccgtat ttaaacaaga
     3121 atgaacaacg ttatgcaaca ttgtgtggta gagacactgt acagtatgaa aatgcatcaa
     3181 ttacacacga cattcttgtt caatttttaa aacaacatca actaaattat cgcagtaatt
     3241 cgtatatggt tatgtttgaa tttaaaggac accgcattgt tgcttttaaa ggtggaaggt
     3301 ttttaataca tggcatgaca cgcacatcag atgccacaca tctaatgaat ttattgtttg
     3361 gataaaaaaa gataagacaa aaggagtgta atattatggg cgaacatcaa aacgttaaat
     3421 tgaatcgtac agttaaagca gccgtactaa cggtatcaga tactagagac tttgttacag
     3481 ataaaggtgg tcaatgcgtg cgccaactat tacaagcaga tgacgttgaa gtgagtgacg
     3541 cacattatac aattgtgaaa gatgaaaaag tagccatcac gacgcaggtg aagaagtggt
     3601 tagaagaaga tattgatgtc atcattacga ctggtggaac aggtattgca caacgtgatg
     3661 tgacgattga agcagtaaaa ccacttttaa ctaaagagat agaaggcttt ggggaattgt
     3721 ttagatattt gagttatgtt gaagatgttg gcacgcgtgc attattgtct cgtgctgtag
     3781 caggtacagt taataataaa ttgatatttt cgattccagg atcaacaggc gcagttaaat
     3841 tagcattaga aaagctcatt aaaccagaat taaatcatct gattcatgag cttacaaaat
     3901 aatttattga cctgattggc gttgaaaatc tccagattta ccgccagact tgctttcaag
     3961 gtaggtttcg ccaataatca tacctttatc aactgctttc gtcatgtcgt aaatggttaa
     4021 agccgttgct gatgcagcgg ttaaagcttc catttcaaca ccggttttgc cagttgtaga
     4081 gacagttgtt tgaatgttta aagtataaag gggtgcattt gtttcatccc agctgaagtg
     4141 aacatctatg ccagtcaatg gtaatggatg gcacatcgga ataattgttg atgtattttt
     4201 ggcagccata ataccagcga tttgagcagt gttcaataca ttgcctttgg tattggtgtt
     4261 attagaaatc tgcttaaaaa tagtttcatt cacagtaatg cttgaatgag cgacagcagt
     4321 tctttttgta atttgtttgt ctgatacatc gaccattttg gcgtggcctt gttgattaat
     4381 atgagtaaac tcagtcattt tacccctcct agtgcatcta gtatatcatg aaaaaataaa
     4441 agttttggag atgattttta atggtagtag aaaaaagaaa cccaatccca gttaaagaag
     4501 caattcaacg tatcgttaat cagcagagtt caatgccggc aattacggta gcacttgaaa
     4561 aaagtctaaa tcatatctta gcagaagata ttgtagctac ttatgatata ccaaggtttg
     4621 ataaatcacc ttatgatggt tttgcaattc gcagtgttga ttcacaaggg gcaagtggtc
     4681 agaatcgcat tgagtttaaa gtgattgatc atattggtgc aggttcagtt tctgataaat
     4741 tagttgggga tcacgaagcg gtgcgtatta tgactggagc acaaatacca aatggcgcag
     4801 atgctgttgt tatgtttgaa caaacgattg aactagaaga tacatttaca attcgtaaac
     4861 cattttcaaa aaatgaaaat atatctttaa aaggtgaaga aacaaagaca ggcgatgttg
     4921 ttctaaaaaa aggacaagta attaacccag gggctatcgc ggtccttgca acatatggct
     4981 atgcagaggt taaagttatt aagcaaccga gtgtcgctgt tattgcaaca ggaagcgaat
     5041 tattagatgt taatgatgta ttagaagatg gaaaaattcg taactctaat ggcccaatga
     5101 ttcgtgcctt agcagaaaaa ttaggtcttg aagttggtat ttacaaaaca caaaaagatg
     5161 atttagatag tggcatccaa gtcgttaaag aagctatgga aaaacacgat atcgttatta
     5221 caacgggtgg agtttctgtt ggagactttg actatttacc tgagatttat aaggctgtaa
     5281 aggcggaagt gttatttaat aaagtagcaa tgcgtcctgg tagcgtaaca acggttgcat
     5341 ttgcagatgg aaagtatttg tttggattat ctggaaatcc atcagcttgt tttacaggat
     5401 ttgaactatt tgtgaaacca gctgttaaac atatgtgtgg cgcactagaa gtcttcccgc
     5461 aaataattaa agcaacatta atggaagatt ttaccaaggc aaacccattc acacgattta
     5521 tacgtgctaa agcaacgtta acaagtgctg gagctactgt agtaccttca ggattcaata
     5581 aatcaggtgc ggttgtagcg attgcacatg ctaactgtat ggtcatgtta ccaggagggt
     5641 cacgtggttt taaagcgggg catacagtag atattatatt gactgaatct gacgctgctg
     5701 aagaggaact tcttttatga ttttacaaat tgtaggttac aaaaagtctg gtaagacaac
     5761 attgatgagg catattgtct ctttcttaaa gacacatggt tatacagttg ctactattaa
     5821 acatcatggg catgctaagg aagatattca attacaggat tcagacgtcg atcacatgaa
     5881 gcattttgaa gcgggggcag atcaaagtat tgtacaaggt tttcaatatc agcaaactgt
     5941 aacacgtgta gataatcaaa atcttactca aattattgag aaatctgtta caattgacac
     6001 caatatcgta ttagttgaag gctttaaaaa tgctgatttt gaaaaagtcg tagtctatcg
     6061 agatgaagaa gagttgcaag tattacaaca attgtcgaat gtttgttata gcattaatgt
     6121 aagggagcat gaagatttta cagcatttga gcaatggtta ttaaataaaa ttaaaaatga
     6181 ttgtgataca caattaacat agaggattga aatgaatgaa acaatttgaa atcgtgatag
     6241 aaccgataca aacagaacaa tatcgtgaat tcactataaa tgaatatcaa ggtgcagtag
     6301 ttgtttttac cggtcatgtt cgcgaatgga ctaaaggcgt caaaacggaa tatttagaat
     6361 atgaagcgta tattccaatg gctgaaaaga aattggcaca aattggagat gaaataaatg
     6421 aaaaatggcc tggaacgata acgagtattg ttcatagaat agggccatta caaatttcag
     6481 atatcgctgt attaattgca gtttcttcac cgcatcgtaa agatgcctat cgagcaaatg
     6541 aatatgcaat tgagcgtata aaagaaattg ttccgatttg gaaaaaagaa atttgggaag
     6601 atggttcaaa atggcaaggg catcaaaaag ggaattatga agaagcgaag agggaggaat
     6661 aagagagatg aaggtacttt acttcgcaga aattaaagat atattacaaa aagcacagga
     6721 agatattgtg cttgaacaag cattgactgt acaacaattt gaagatttat tgtttgaacg
     6781 ttatccgcaa atcaataata aaaagtttca agttgctgta aatgaggaat ttgtacaaaa
     6841 atcagatttc attcaaccta atgatactgt tgcattaatt ccaccggtta gtggaggtta
     6901 agggagcatg aaagcaataa ttcttgcagg tggtcattca gtgcgatttg gtaagcccaa
     6961 agcttttgcg gaagtgaacg gtgagacctt ttatagtaga gtaattaaga cattagaatc
     7021 aacaaatatg ttcaatgaaa ttattattag tacaaatgcg caattggcaa cgcaatttaa
     7081 atatccaaat gttgttatag atgatgagaa tcataatgat aaaggtccat tagcaggaat
     7141 ttatacaatc atgaagcaac atcctgaaga agaattgttt tttgtcgttt ctgttgatac
     7201 accaatgatt actggtaaag ctgtaagcac gttgtatcag tttttagttt ctcatcttat
     7261 tgaaaatcat ttagatgtcg cagcttttaa agaagatgga cgttttattc caacaattgc
     7321 attttatagt ccgaatgcat taggcgctat aactaaagca ctacattctg ataattacag
     7381 ttttaaaaat atatatcatg aattatcaac ggattatttg gatgtcaggg atgtagatgc
     7441 gccctcatat tggtacaaaa atataaatta tcagcatgat ttggacgctt taattcaaaa
     7501 attgtaagct gttaggaggt ccacaaatgg tagaacaaat aaaagataaa ctaggacgtc
     7561 ccatccgtga cttacggtta tctgtgacag atcggtgtaa ctttaggtgt gattattgca
     7621 tgcctaaaga ggtatttgga gatgatttcg tatttttacc taaaaatgaa ctgttaacgt
     7681 ttgatgaaat ggctagaatc gctaaggtat atgcagaatt aggtgtaaaa aaaatacgca
     7741 ttacaggtgg agaaccattg atgcgacgcg atttagatgt acttatagct aaattaaatc
     7801 aaatcgatgg tattgaagat attggtttga ctacaaatgg tttgttatta aaaaagcatg
     7861 gacaaaagtt atatgatgct gggctacgca gaattaatgt cagtttggat gctattgatg
     7921 atacgctatt tcaatcaatc aataatcgta atattaaagc gactacgatt ttagaacaaa
     7981 ttgattacgc gacgtctatt ggtttgaatg taaaagtaaa tgttgttata caaaaaggta
     8041 ttaacgatga tcaaatcata ccaatgcttg aatattttaa agataaacat atagagattc
     8101 gatttataga atttatggat gttggtaatg ataatggatg ggatttcagt aaagttgtaa
     8161 ctaaagatga aatgcttaca atgatagagc agcactttga aatcgatcct gtagaaccaa
     8221 aatattttgg ggaagtagca aaatattatc gccataagga taatggtgtt caatttggtt
     8281 tgattacaag tgtttcacaa tcattttgtt ctacatgtac acgcgcaagg ctgtcatcag
     8341 atgggaagtt ttacggatgt ttatttgcaa ctgtcgatgg atttaacgtt aaagcgttta
     8401 ttcgttctgg cgtgaccgac gaagaattaa aagaacaatt taaagcttta tggcaaataa
     8461 gagatgatcg atattcagat gagagaactg ctcaaacagt tgccaatcgt caacgtaaaa
     8521 agataaacat gaattatatt ggtggttaat gtgtagggac cactacatat taaatcatta
     8581 gagatgtttt aatatttctg tcttactccc taaaatacaa tattatttat taaagtaaaa
     8641 acggtcatat ctatgccaga tttaatagaa atgatcgttt ttaaagtttt tacaagttgg
     8701 cggggcccca acacagaagc tgacagaaag tcagcttaca aaaatgtgca agttggcggg
     8761 gccccaacat agagaatttc aaaaagaaat tctacagaca atgcaagttg gggatcaacg
     8821 aaataaattt tgtgaaaatg ttatttctgt cccactccca aaaatcacta cataattaat
     8881 ctttaagggc tctttaacat tgatgtcaca ctccaagtta ttgagttgta atatatcttt
     8941 tttaggtata aatgttgtcg aataaacaac aagttgtcca aaagatataa atctaaacaa
     9001 gatatagcca gcaatttaat atttgtaata gataaaatgc taagtttgat atataataaa
     9061 tttaagtaat tgtataataa tatgaattac aaacatctaa gaagaaacat aggaggcatc
     9121 atattatgag taataaagtt caacgtttta tagaagcaga aagggagtta agtcagttaa
     9181 agcactggtt aaaaacaaca cataagattt caattgaaga atttgtagta ctttttaaag
     9241 tgtatgaagc tgaaaagatt agcggtaaag aattgaggga tacattacat tttgaaatgc
     9301 tatgggatac aagtaaaatc gatgtgatta tccgtaaaat ctataaaaaa gagcttattt
     9361 ctaaattgcg ttctgaaacg gatgaaagac aagtattcta tttctatagt acttctcaaa
     9421 agaaattgtt agataaaatt actaaagaaa tagaagtgtt aagcgttaca aactaaaaac
     9481 ttaaaaagca tgccaatcag tacatcatat ttgcgtcttg gggacagaca aatgatgaat
     9541 agagattggc atgcttttta tttttgaata taaatattta gttcatggca tttctagtta
     9601 catgacgtcc atgaattaag aagtaaacaa gcatagtaat gattgctaaa gcggccataa
     9661 agccgaagat ttcactatat gaaaacatat gagtaaataa cccaaggaat gatggaccga
     9721 agccgacacc tgcatctaga ccaacgtaaa aagtagatgt cgcgatacca tatttaatcg
     9781 ggggtgagac ttttatcgca atagattgca ttgcagatga taaatttcca taccctaaac
     9841 ctaggcaagc accagcaagt aatattaacc agctttgata gcttgaaatt aagcacacaa
     9901 atgaaaggaa aagcatgata aatgctggat agacaataat attttcattt ttatcatcca
     9961 tcaatctacc agcaataggt ctagtaatta acgatgctat agcatagcaa ataaagaaat
    10021 agcttgctgc agtgactagg tgtcgctcta aagcaaatgc ttgtaaataa gttaggattg
    10081 acgcataggt aacgccaatt aaaagcataa ttacagcaac aggaatggcc tcttttgcaa
    10141 taaattgatg aatactaaat cttggtttat taatgacatc agtttcagtt ttattatttg
    10201 ttacttcgaa atcaactttt ataaataatg agataatgag tccgagtatg cctaatatga
    10261 cacaaataat aaacagtaaa tcaattgcgt attttgtaat aagtaacatg cctagaaatg
    10321 ggccaatcgc tgtacctaat actaaactta aggaaaataa actgatgcct tcactttttc
    10381 tattaacagg ggtaacgtat gccgcaatag tacctgttgc agttgtcaca actgcagttg
    10441 cgataccgtt tatgagacgt acaaagatta aaaaagctaa agatccatca ataaaataaa
    10501 gtaattgcgt gataattaaa gcaattaaac caataaataa taatcgttta ggtccgattt
    10561 gatttacaaa tttacctgta gcaaatcgac caattaaaga cccgacaata aatagtccta
    10621 caactaatcc ggctaatgaa tcggaagcgt cgaaagcttg cttactatag ccagcaataa
    10681 ctacaagtaa caaatacatg cataggtata cgataaagtt gacaaaaaag ttgattgtaa
    10741 aacttttggt gaaaataggt gatttattta atgagccttg attagtcatt gtgtgcctcc
    10801 tttaataata tttcatgaat ttgattcatc acatttatgg tttcttgcat ttgatcttgc
    10861 gaaatgtttg ttttggcaac tactgcttgt tggagctttt gtacatcaag acaaacaatc
    10921 tcatataatt catgcccttt aggtgttaaa gttaaaaact tttgtcgttt atcctctaaa
    10981 ctgttttcta ctgttataag gtcattttct attagagctt ttaaagtttt tcttgcagta
    11041 ggcttttcaa ttgcacgtct atgtgaaatt tcaatgagag tagtgggctg atgtttagcg
    11101 atatcgcgta agattaacca ttgaccataa tatatattgt gtttttccaa aatcggctct
    11161 gctaatttta aataggggcg atatattgtt ataaaactat tgaaaaattc ttgtgatagc
    11221 atagtgacat ctcctaagac aaaatagtta gcttagctaa cctttttaca acaatagtaa
    11281 ttataaa
//
LOCUS       DAHHSC010000068        11182 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.068, whole genome shotgun sequence.
ACCESSION   DAHHSC010000068 DAHHSC010000000
VERSION     DAHHSC010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 11182)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11182)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11182
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.068"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            18..971
                     /locus_tag="NBI57_001366"
     CDS             18..971
                     /locus_tag="NBI57_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1822319.1"
                     /translation="MKKTLGCLLLIMLLVVAGCSFGGNHKLSSKKSEESKQETVKKES
                     EEEKDPDLEKYEEIEKKMKGIKDAPSLDKLDPLMTEKSFTNSKGIQGWKDYKELMGKV
                     ELADYRFTKDSKGSSIKDVDAFFKGKKGIKRKVIETHDDVKQVDYWYVDPDGKKIGNS
                     NTPVFYAEIMTKYKDGKLVYASVEPGSYVIHKDDAIKYDDYSKLKKLSQLTKLDHPKP
                     VPYSVAQIKSFGVPLTSVSFMTHGSKDTKDEVLPALAYFTFSPKNYEDKSNPDPKVLN
                     LVHMDFLNASSDFGNAHFVVLSKYIKEYESNYETASDDSLK"
     gene            complement(1134..1337)
                     /locus_tag="NBI57_001367"
     CDS             complement(1134..1337)
                     /locus_tag="NBI57_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001830141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TM2 domain-containing protein"
                     /protein_id="HCD1822320.1"
                     /translation="MQVNKVIYILLALFLGSFGIHKFYAGKNMQGILHLIFCWTGIPH
                     ILAIISAVITVFKPADEQGNVTL"
     gene            1636..2124
                     /locus_tag="NBI57_001368"
     CDS             1636..2124
                     /locus_tag="NBI57_001368"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HCD1822321.1"
                     /translation="MAIKKLVPASHPILTKKAQAVKTFDDSLKRLLQDLEDTMYAQEA
                     AGLCAPQINQSLQVAIIDMEMEGLLQLVNPKIISQSNETITDLEGSITLPDVYGEVTR
                     SKMIVVESYDVNGNKVELTAHEDVARMILHIIDQMNGIPFTERADRILTDKEVEAYFI
                     ND"
     gene            2117..3052
                     /locus_tag="NBI57_001369"
     CDS             2117..3052
                     /locus_tag="NBI57_001369"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HCD1822322.1"
                     /translation="MTKIIFMGTPDFSTTVLEMLIAEHDVIAVVTQPDRPVGRKRVMT
                     PPPVKKVAMKYDLPVYQPEKLSGSEELEQLLQLDVDLIVTAAFGQLLPESLLALPKLG
                     AINVHASLLPKYRGGAPIHQAIIDGEQETGITIMYMVKKLDAGNIISQQAIKIEENDN
                     VGTMHDKLSVLGADLLKETLPSIIEGTNESVPQDDTQATFASNIRREDERISWNKPGR
                     QVFNQIRGLSPWPVAYTTMDDTNLKIYDAELVETNKINEPGTIIETTKKAIIVATNDN
                     EAVAIKDMQLAGKKRMLAANYLSGAQNTLVGKKLI"
     gene            3049..4356
                     /gene="rsmB"
                     /locus_tag="NBI57_001370"
     CDS             3049..4356
                     /gene="rsmB"
                     /locus_tag="NBI57_001370"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HCD1822323.1"
                     /translation="MIENVRSLAFDTIQDILNEGAYSNLRINEVLSENELNAMDKALF
                     TEIVYGTVKRKYTLDFYLKPFVKTKIKAWVRQLLWMSIYQYVYLDKVPNHAIINEAVE
                     IAKERGGYHNGNVVNGILRTMMRSDLPDFNEIADPKKRMAIEYSMPKWIIDHWATHYG
                     LEETETILQSFLETTSTTVRANLTRASLDDIIEKLQDEGYDVEKDHDLPYCLHIGGQP
                     IIHSRSFKDGFVSIQDKSSMFVAHIMNVDRHDHVLDACSAPGGKACHIAEVLMPEGQV
                     DASDIHDHKIDLINFNIKKLRLTNIKAFQHDATKPYDKTYDKILVDAPCSGLGVMRHK
                     PEIKYTQSKQHIESLVELQLEILENVKNNVKIGGEIIYSTCTIEQLENENVIYTFLKN
                     NKNFEFEPFQHPITGELVKTLQIMPQDFNSDGFFITKIKRKDN"
     gene            4359..5453
                     /gene="rlmN"
                     /locus_tag="NBI57_001371"
     CDS             4359..5453
                     /gene="rlmN"
                     /locus_tag="NBI57_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002435140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HCD1822324.1"
                     /translation="MITAEKKKKNKFLPNFDKQSIYSLRFDEMQNWLVEQGQQKFRAK
                     QIFEWLYQKRVDSIDEMTNLSKDLRQLLKDNFTVTTLTTVVKQESKDGTIKFLFELQD
                     GYTIETVLMRHDYGNSVCVTTQVGCRIGCTFCASTLGGLKRNLEAGEIVSQVLTVQKA
                     LDATEERVSQIVIMGIGEPFENYDEMMDFLRIVNDDNSLNIGARHITVSTSGIIPRIY
                     DFADEDIQINFAVSLHAAKDEVRSRLMPINRAYNVEKLIEAIQYYQEKTNRRVTFEYG
                     LFGGVNDQLEHARELAHLIKGLNCHVNLIPVNHVPERNYVKTAKNDIFKFEKELKRLG
                     INATIRREQGSDIDAACGQLRAKERQVETR"
     gene            5460..6203
                     /locus_tag="NBI57_001372"
     CDS             5460..6203
                     /locus_tag="NBI57_001372"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase Stp1"
                     /protein_id="HCD1822325.1"
                     /translation="MLEAQFFTDTGQHRDKNEDAGGIFYNQTNQQLLVLCDGMGGHKA
                     GEVASKFVTDELKSRFEAENLIEEHQAENWLRNNIKDINFQLYHYAQENAEYKGMGTT
                     CVCALVFEKSVVIANVGDSRAYVINSRQIEQITSDHSFVNHLVLTGQITPEEAFTHPQ
                     RNIITKVMGTDKRVSPDLFIKRLNFYDYLLLNSDGLTDYVKDNEIKRLLVKEGTIEDH
                     GDQLMQLALDNHSKDNVTFILAAIEGDKV"
     gene            6200..8194
                     /gene="pknB"
                     /locus_tag="NBI57_001373"
     CDS             6200..8194
                     /gene="pknB"
                     /locus_tag="NBI57_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein kinase Stk1"
                     /protein_id="HCD1822326.1"
                     /translation="MIGKIINERYKIVDKLGGGGMSTVYLAEDTILNIKVAIKAIFIP
                     PREKEETLKRFEREVHNSSQLSHQNIVSMIDVDEEDDCYYLVMEYIEGPTLSEYIESH
                     GPLSVDTAINFTNQILDGIKHAHDMRIVHRDIKPQNILIDSNKTLKIFDFGIAKALSE
                     TSLTQTNHVLGTVQYFSPEQAKGEATDECTDIYSIGIVLYEMLVGEPPFNGETAVSIA
                     IKHIQDSVPNVTTDVRKDIPQSLSNVILRATEKDKANRYKTIQEMKDDLSSVLHENRA
                     NEDVYELDKMKTIAVPLKKEDLAKHISEHKSNQPKRETTQVPIVNGPAHHQQFQKPEG
                     TVYEPKPKKKSTRKIVLLSLIFSLLMIALVSFVAMAMFGNKYEETPDVIGKSVKEAEQ
                     IFNKNNLKLGKISRSYSDKYPENEIIKTTPNTGERVERGDSVDVVISKGPEKVKMPNV
                     IGLPKEEALQKLKSLGLKDVTIEKVYNNQAPKGYIANQSVTANTEIAIHDSNIKLYES
                     LGIKQVYVEDFEHKSFSKAKKALEEKGFKVESKEEYSDDIDEGDVISQSPKGKSVDEG
                     STISFVVSKGKKSDSSDVKTTTESVDVPYTGKNDKSQKVKVYIKDKDNDGSTEKGSFD
                     ITSDQRIDIPLRIEKGKTASYIVKVDGKTVAEKEVSYDDI"
     gene            8422..9297
                     /gene="rsgA"
                     /locus_tag="NBI57_001374"
     CDS             8422..9297
                     /gene="rsgA"
                     /locus_tag="NBI57_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HCD1822327.1"
                     /translation="MKTGRIVKSISGVYQVDVNGERFNTKPRGLFRKKKFSPVVGDIV
                     EFEVQNINEGYIHQVFERKNELKRPPVSNIDTLVIVMSAVEPNFSTQLLDRFLVIAHS
                     YQLNARVLVTKKDKTPIEKQFEINELLKIYENIGYETEFIGNDDDRKKIVEAWPAGLI
                     VLSGQSGVGKSTFLNHYRPELNLETNDISKSLNRGKHTTRHVELFERQNGYIADTPGF
                     SALDFDHIDKDEIKDYFLELNRYGETCKFRNCNHIKEPNCNVKHQLEIGNIAQFRYDH
                     YLQLFNEISNRKVRY"
     gene            9298..9942
                     /locus_tag="NBI57_001375"
     CDS             9298..9942
                     /locus_tag="NBI57_001375"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HCD1822328.1"
                     /translation="MTKLYPSLLSVDFLDLQHELKRLEEAGVDGVHFDVMDGQFVPNI
                     SIGLPILDAVRKGTTLPIDVHLMIENPEKYIASFAEHGADMISIHVESTPHIHRAIQM
                     IKHLDKKAGVVINPGTPISQIEPILDIVDYVLVMTVNPGFGGQSFIDQCVEKIAGLNA
                     IKMERQLNFDIEVDGGVNTDTAKVCVENGATMLVTGSFFFKQEDYKKVTQALKG"
     gene            9949..10590
                     /locus_tag="NBI57_001376"
     CDS             9949..10590
                     /locus_tag="NBI57_001376"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HCD1822329.1"
                     /translation="MHINLLCSNRHLPQDIWAKSNEGKWGGVDRGALILLKHQIIPFF
                     SVGDFDSVSKEERQLLTEQLQIKPVQAEKADTDLALAVDKAVALGFDSITIYGATGGR
                     LDHFFGAIQLLLKKAYYKHDVHIEVIDQQNKIELLPKGQHTVEKDKSYPYISFIPMTD
                     DVELSLAGFKYNLARQMLNIGSTLTISNEIESLQAKVTVHDGLILQIRSTDLN"
     gene            complement(10971..11159)
                     /locus_tag="NBI57_001377"
     CDS             complement(10971..11159)
                     /locus_tag="NBI57_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014614133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HCD1822330.1"
                     /translation="MGKQCFVTGRKASTGNRRSHALNSTKRRWNANLQKVRILVDGKP
                     KKVWVSARALKSGKVTRV"
BASE COUNT         4151 a         1542 c         2097 g         3392 t
ORIGIN      
        1 ttaagaggag aaagtttatg aaaaagacac tgggatgttt acttttaatt atgcttttag
       61 tcgtagcagg ttgttctttt ggtgggaatc ataaattatc atcaaagaaa tcagaagaat
      121 caaaacaaga aactgtaaaa aaagaatcgg aagaagagaa agatccagat ttagagaaat
      181 atgaagaaat agagaagaaa atgaaaggaa ttaaagatgc gccatctctt gataagttgg
      241 atccattaat gacagaaaag tcgtttacga atagtaaagg gattcaagga tggaaagatt
      301 acaaagaatt aatgggtaaa gtggaacttg cagattatag atttactaaa gattcaaaag
      361 gatcttcaat aaaagatgtt gatgcattct ttaaaggtaa gaaaggtata aaaaggaaag
      421 tgattgaaac acacgatgat gtaaaacaag ttgattattg gtatgtagat ccagatggaa
      481 agaaaattgg caattcaaac acacctgttt tttacgcaga aattatgaca aaatataaag
      541 atggaaagtt agtttatgca tcagtcgaac caggatctta cgtaatacat aaagatgatg
      601 caattaaata tgacgattat tctaagttaa aaaaattaag tcagctaact aaacttgatc
      661 atccaaaacc agttccatat agcgtagctc aaatcaaatc tttcggagta cctttaacaa
      721 gcgtttcatt tatgacacat ggatcaaagg atactaaaga tgaagtgttg ccggcattgg
      781 cctatttcac tttttcacca aaaaattatg aagacaagtc taatccagat ccaaaagttt
      841 taaatttagt acatatggat ttcttaaatg catctagtga ttttggtaac gcacattttg
      901 ttgttttaag taaatatatt aaagagtatg aatcaaacta tgaaacagcg tcagatgatt
      961 ctttaaaata gtatttactg tgtgaaaaat aaatagtgta ctacattaaa taatcgcaat
     1021 aataatcccg ataaacaatc agcattactg cttatcatat agagttcgta ataactataa
     1081 ctctatgatt cgcaaataat aaatgattgt catcgggatt tatttttatc aatttataaa
     1141 gtgacattac cttgttcatc agcaggtttg aaaacagtaa tcactgcact aataattgct
     1201 aaaatgtgtg ggatacctgt ccaacagaat attaagtgta gaataccttg catattcttg
     1261 ccggcataaa atttatgaat accaaaacta cctaagaaca atgctaataa aatataaata
     1321 actttgttta cttgcatttc tttccctcca gttgaattgc ttataatgtc attagcttct
     1381 ctttttatta tacccacttt tagttcaaac attctagttt aagcattccc aatcatctaa
     1441 atttcagtta ttcattcctt acaataaatt taggattaca cttcagttgc attgtattat
     1501 tttacgtgtg aaatatacgt aatgaatcac atgacaatct tcaaattgaa aaatatacat
     1561 tctatgatgt aaggtcgcat ttttaatata tttacgttat aatagtttga tgaattttat
     1621 tgaggagtaa gagttatggc gattaaaaag ttagtaccag catcgcatcc tattttaacg
     1681 aaaaaagcgc aagcagttaa aacatttgat gattcgttaa aaagattatt acaagattta
     1741 gaagatacaa tgtatgcaca agaagctgct ggcttatgtg cacctcaaat taatcagtca
     1801 ttgcaagtgg caattattga tatggaaatg gaaggattat tacaacttgt taatccgaaa
     1861 attattagtc aatcaaatga aacaataaca gacttagaag gttcaattac attgccagat
     1921 gtttacggcg aagtgacaag aagtaaaatg atagttgttg aaagttatga cgtcaatggg
     1981 aacaaagttg aactaactgc acatgaagat gtagcaagaa tgattttgca tattatagat
     2041 caaatgaacg gtatcccttt tacagaacgt gcggaccgta ttttaacaga taaagaagtg
     2101 gaggcatatt ttataaatga ctaaaataat atttatgggt acaccagact tttcaacaac
     2161 tgttttagaa atgcttattg cagaacatga tgtcattgca gtcgtaacgc aaccagatcg
     2221 acctgttgga cgtaaacgtg ttatgacacc accaccagtt aaaaaagttg caatgaaata
     2281 tgatttacct gtatatcagc ctgaaaaatt aagtggatca gaagaattag aacaattgct
     2341 tcaattagat gtagatttaa ttgtaactgc tgcttttgga caattattac ctgaatcatt
     2401 gttggcatta ccaaaacttg gcgcaattaa tgtacatgca tcattgttac caaagtatag
     2461 aggtggtgca ccaattcatc aggcaattat cgatggtgaa caagaaaccg gcataacaat
     2521 tatgtatatg gttaaaaaat tagatgcggg taatattatt tcgcaacaag caattaaaat
     2581 agaagaaaat gataatgtcg gtacgatgca tgataaatta agtgtattag gggcagattt
     2641 attaaaagaa actttaccat ctattataga gggcacaaat gaaagtgtac ctcaagatga
     2701 tacgcaagca acatttgctt ccaatattcg acgcgaagat gagcgaatta gctggaataa
     2761 accaggaaga caagtgttta atcaaattcg tggattatca ccatggccag ttgcttatac
     2821 aactatggat gacactaact tgaaaatata cgatgctgaa ctcgttgaga ctaataagat
     2881 aaacgagcct ggaaccatta tagaaacgac taaaaaagcc attattgttg ctacaaatga
     2941 taatgaagct gttgcaatta aagatatgca attagctggg aaaaagagaa tgttagctgc
     3001 caattattta agtggtgcgc aaaacacact agtagggaag aaacttatat gatcgaaaac
     3061 gtgagaagtc ttgcttttga cacgattcaa gatatattaa atgaaggtgc gtatagtaac
     3121 ttgcgtatca atgaagtgtt gtcagaaaat gaattaaatg caatggataa ggctttattt
     3181 acagaaattg tctacggaac cgttaaaaga aaatatacgt tagattttta tttaaagcct
     3241 tttgtgaaaa caaagattaa ggcatgggtt aggcaattat tatggatgag tatttatcaa
     3301 tatgtatatt tagataaagt tccaaatcat gccattatta atgaagcagt tgaaatagca
     3361 aaagaacgcg gtggctatca taatggtaat gtcgtaaatg gtattttacg tacaatgatg
     3421 cgtagtgact tacctgattt taatgaaatt gcagatccta aaaaaagaat ggcaatcgag
     3481 tatagtatgc cgaagtggat tatagatcat tgggcaacac attatggtct cgaagaaact
     3541 gaaacaattt tacagtcatt tttagaaacg acatcaacaa ctgtgcgtgc caacctgacg
     3601 cgagcatcat tagatgatat tattgaaaag ttgcaagacg aaggttatga cgttgaaaaa
     3661 gatcatgact taccttattg tctccatata ggaggacaac caattattca ttctcgttca
     3721 tttaaagatg gattcgtttc aattcaagat aaaagctcaa tgtttgttgc acacattatg
     3781 aatgtagacc gacatgatca cgtattagat gcatgtagtg cacctggcgg taaagcttgt
     3841 cacattgctg aagttttaat gccagaaggg caagttgacg cttcagatat acatgatcac
     3901 aaaatagact taattaattt taatataaaa aaattacgat taacaaatat taaggctttt
     3961 caacatgatg cgacaaaacc ttatgataaa acatacgata agatacttgt tgatgcacca
     4021 tgtagcggat taggtgtaat gagacataag ccggagatta agtatactca aagcaaacaa
     4081 catattgagt cactagttga attacagctt gaaatattgg aaaatgtaaa aaacaatgta
     4141 aaaataggtg gcgaaatcat ctattcaaca tgtacaattg agcaactaga aaatgaaaac
     4201 gtgatttata cgtttctgaa aaataataaa aacttcgaat ttgaaccgtt tcaacatccg
     4261 ataactggag agttggtcaa aacgttacaa atcatgccgc aagactttaa ttcagatgga
     4321 ttctttatca ctaagataaa aagaaaggac aattaggaat gataactgct gaaaagaaaa
     4381 agaagaataa atttcttcca aattttgaca agcaatcaat atattcattg cgatttgacg
     4441 aaatgcaaaa ctggctcgtt gaacaaggtc aacaaaaatt tcgagcgaaa cagatttttg
     4501 aatggttata tcaaaaaaga gtagattcga ttgatgaaat gacgaactta tcgaaagact
     4561 tacgacagct tttaaaagat aactttactg ttacaacttt aacaactgta gtaaaacaag
     4621 aaagtaaaga cggtacaatt aaattcttat ttgaattaca agatggctat acaattgaaa
     4681 ctgttttaat gagacatgat tatggaaatt cagtatgtgt aacgacacaa gtaggttgtc
     4741 gtatcggatg tacgttttgt gcttctacac ttggcggctt aaaaagaaac cttgaagctg
     4801 gcgaaattgt ttcacaagtt ttaacagttc aaaaagccct tgatgctaca gaagagcgcg
     4861 tatctcaaat tgtcataatg ggtatcggtg aaccatttga aaattatgat gaaatgatgg
     4921 actttttaag aatcgtcaat gatgataaca gtttaaatat tggtgcacgt cacattacag
     4981 tatcaacatc aggtatcatt cctagaatat atgactttgc ggatgaagat atccaaatta
     5041 attttgctgt aagcttacac gccgcaaaag atgaagtgcg atcacgcttg atgccaatta
     5101 accgtgcata taatgttgag aagttaatcg aagcaattca atattatcaa gaaaaaacaa
     5161 atcgtcgtgt tacttttgaa tatggtctgt ttggtggtgt gaatgaccaa ctagaacatg
     5221 caagagaatt agcacattta ataaaaggct taaactgcca tgttaactta attcctgtca
     5281 accatgttcc agaaagaaat tatgtgaaaa cggctaaaaa tgatatcttt aaatttgaaa
     5341 aagaattaaa gagactagga attaatgcca caatacgtcg tgaacaaggt tcggatattg
     5401 acgcagcttg tggtcaatta agagcaaagg aacgacaagt agaaacgagg taaagacaaa
     5461 tgctagaggc acaatttttt actgatactg gacaacatag agataagaat gaagatgcgg
     5521 gtggtatttt ttataatcaa actaatcaac aacttttagt tctgtgtgat ggtatgggcg
     5581 gccataaagc aggagaagtt gcaagtaaat ttgttacaga tgagttgaaa tcccgttttg
     5641 aagcggaaaa tcttatagaa gaacatcaag ctgaaaattg gttgcgtaat aatataaaag
     5701 atataaattt tcagttatat cactatgcac aagaaaatgc agaatataaa ggtatgggta
     5761 caacatgtgt ttgtgcactt gtttttgaaa aatcagttgt gatagcaaat gtcggtgatt
     5821 ctagagccta tgttattaat agtagacaaa ttgaacaaat tactagtgat cactcatttg
     5881 ttaatcatct tgttttaacg ggtcaaatta cgccggaaga agcatttaca catccacaac
     5941 gtaatattat tacgaaggtg atgggcacag ataaacgtgt gagtccagat ttgtttatta
     6001 agcgattaaa tttttatgat tatttattat taaattcaga tggattaact gattatgtta
     6061 aagacaatga aattaagcgt ttgttagtaa aagaaggtac aatagaagat catggtgatc
     6121 aattaatgca attggcatta gataaccatt cgaaagataa cgttactttc atactcgcgg
     6181 ctattgaagg tgataaagta tgataggtaa aataataaat gaacgatata aaattgtaga
     6241 taagcttggc ggcggtggca tgagtaccgt ttatcttgca gaagatacga tacttaacat
     6301 taaagttgca attaaggcga tttttatacc acctagagaa aaagaagaaa cattaaaacg
     6361 ttttgaacga gaagtacata actcatcaca gctatcacat caaaatatag taagtatgat
     6421 cgatgttgat gaagaagatg actgttacta cttagtaatg gaatatattg aaggtccgac
     6481 tttgtctgag tatattgaaa gtcatgggcc attaagtgtt gacacggcga ttaattttac
     6541 gaatcaaata ttggatggca ttaaacatgc gcatgatatg cgtattgtac atagagatat
     6601 taagccacaa aatatattaa ttgacagcaa taaaacgttg aaaatatttg attttggaat
     6661 tgctaaagct ttaagtgaga cgtctttaac tcagactaat catgtgttag gtactgtgca
     6721 gtacttttcg ccagaacaag caaaaggtga ggcaacggat gaatgtacag atatttattc
     6781 tataggtatt gtgttatatg aaatgcttgt tggtgaacca ccctttaatg gagaaactgc
     6841 agttagcatt gcgattaaac atattcagga ttctgtgcca aatgtgacaa cagatgtacg
     6901 taaggatatt ccgcaatctt taagtaatgt cattttacgc gctacagaaa aagacaaagc
     6961 gaatcgttac aaaacaattc aagaaatgaa agatgatttg agtagtgttt tacatgaaaa
     7021 tcgagcgaat gaagatgtct atgaactcga taaaatgaaa acgatagcgg tacctttgaa
     7081 aaaagaagat ctagcaaagc atattagtga acataagtcg aatcaaccta aacgtgaaac
     7141 gacgcaagta cctattgtaa atgggcctgc tcatcatcag caattccaaa agccagaagg
     7201 tacggtgtac gaaccaaaac ctaaaaagaa atcaacacga aagattgtgc tcttatcact
     7261 aatcttttcg ttgttaatga ttgcacttgt ttcttttgtg gcaatggcaa tgtttggtaa
     7321 taaatacgaa gagacacctg atgtaatcgg gaaatctgta aaagaagcag agcaaatatt
     7381 caataaaaac aacctgaaat tgggtaaaat ttctagaagt tatagtgata aatatcctga
     7441 aaatgaaatt attaagacaa ctcctaatac tggtgaacgt gttgaacgtg gtgacagtgt
     7501 tgatgttgtt atatcaaagg gccctgaaaa ggttaaaatg ccaaatgtca ttggtttacc
     7561 taaggaggaa gcattgcaga aattaaaatc gttaggtctt aaagatgtta cgattgaaaa
     7621 agtatataat aatcaagcgc caaaaggata cattgcaaat caaagtgtaa ccgcaaatac
     7681 tgaaatcgct attcatgatt ctaatattaa actatatgaa tctttaggca ttaagcaagt
     7741 ttatgtagaa gactttgagc ataaatcctt tagcaaagct aaaaaagcct tagaagaaaa
     7801 agggtttaaa gttgaaagta aggaagagta tagtgacgat attgatgagg gtgatgtgat
     7861 ttctcaatct cctaaaggaa aatcagtaga tgaggggtca acgatttcat ttgttgtttc
     7921 taaaggtaaa aaaagtgact catcagatgt caaaacgaca actgaatcgg tagatgtacc
     7981 atacactggt aaaaatgata agtcacaaaa agttaaagtt tatattaaag ataaagataa
     8041 tgacggttca actgaaaaag gtagtttcga cattactagt gatcaacgta tagacattcc
     8101 tttaagaatt gaaaaaggaa aaacagcaag ttatattgtt aaagttgacg gtaaaactgt
     8161 agctgaaaaa gaagtcagct atgatgatat ttaaatataa ttgaagtaaa tgtaccgagg
     8221 tttctatttg gaagtctcgg tatttttatg ttgagattgc ggtagtttta aaatgcttaa
     8281 tgtcatcatt tacagttgag cattgtctat caattctgta tcactctcac aatatttata
     8341 tcatttgaaa tgatcattta tatattaatt ttccagtctt tgatataata aatgcagtaa
     8401 aattaatcga gaggtgccat tttgaagaca ggtcgaatag tgaaatcaat tagtggggta
     8461 tatcaagtag acgttaatgg cgaacgtttc aatacaaaac cacgaggatt atttagaaag
     8521 aaaaaatttt caccggtagt tggtgatata gtggaatttg aagtacaaaa cattaacgaa
     8581 ggctatattc atcaagtgtt tgagcggaaa aatgagttga aaagaccacc tgtaagtaat
     8641 atagatacac tagtaattgt aatgagtgct gtcgagccaa atttttcaac gcaattatta
     8701 gatcgatttt tagttattgc acattcgtat cagttaaatg cgagagtttt ggtgactaaa
     8761 aaagataaaa caccaattga aaagcagttc gaaattaatg agttgttgaa aatatatgaa
     8821 aatattggct atgagactga atttattgga aatgatgatg atcgaaaaaa aattgtagaa
     8881 gcttggccag ctggacttat agtacttagt ggtcaatcag gtgtcggtaa gtccactttc
     8941 ttaaatcatt atcgtccaga acttaatctt gagacaaatg atatatcaaa atcattaaat
     9001 cgaggaaagc atactacaag acatgtcgaa ctattcgaac gtcaaaacgg ttatattgca
     9061 gacacacctg gattcagtgc tttagatttt gatcatatag ataaagatga aataaaagat
     9121 tattttcttg aattaaatcg atatggtgaa acatgtaagt ttaggaattg taatcatatc
     9181 aaagaaccta attgtaatgt taagcatcaa ttagagatag ggaatattgc gcaatttaga
     9241 tacgaccatt atttacaact atttaatgaa atttcaaata gaaaggttag atattaaatg
     9301 acaaaactat atccatcatt attatctgtt gattttttgg atttacaaca tgaattaaaa
     9361 cgacttgaag aagcaggtgt cgacggagtt cattttgatg ttatggatgg tcaatttgtg
     9421 cctaatatat ctattggttt accaatatta gatgcagtaa gaaaaggcac aacattacct
     9481 atagacgtac atttgatgat tgaaaatcca gaaaagtata ttgcatcatt tgcagaacat
     9541 ggtgccgata tgatttcaat tcatgtcgaa tcaacgcctc atattcatcg tgctattcaa
     9601 atgattaaac atttagataa aaaagctggt gtagtaatta atcctggtac accaatatca
     9661 caaattgaac ctattttaga cattgttgat tatgtattag tgatgacagt taacccaggg
     9721 tttggtggtc aatcatttat tgatcaatgc gtagaaaaaa tagcgggtct taatgctatt
     9781 aaaatggaac gtcaattaaa ctttgatatt gaagttgatg gaggcgtaaa taccgataca
     9841 gcgaaagttt gtgttgaaaa tggtgctaca atgctagtaa caggttcatt tttctttaaa
     9901 caagaagatt ataaaaaagt cacacaagca ttgaaaggtt gagtgtatat gcatataaat
     9961 ttattatgtt ctaatcgaca cttgccgcaa gatatttggg ccaaaagtaa tgaaggtaaa
    10021 tggggcggcg ttgatagagg tgctttgatt ttattgaagc atcaaattat cccttttttc
    10081 tcagtgggag actttgattc agtcagtaaa gaagaacgcc aacttctaac agaacagtta
    10141 caaatcaaac cagttcaagc tgaaaaagct gatacggatt tagctttagc ggttgataaa
    10201 gctgttgcac ttggatttga tagtattaca atttatggtg caacaggcgg acgattagat
    10261 cacttttttg gggcaattca gttattattg aaaaaagcat attataaaca tgatgttcat
    10321 atagaagtta tcgatcaaca aaataaaatt gaattattgc ctaaaggtca acatacagtt
    10381 gaaaaagata agagttatcc gtacatttca tttataccga tgactgatga tgtagaactt
    10441 tctctagcag gttttaaata taatttagct agacaaatgc ttaatatagg ttctacttta
    10501 actatttcaa atgaaattga gtctttgcaa gcgaaagtaa ctgtacatga tgggttgatt
    10561 ttgcaaatta gaagtacaga tttaaattaa gtatattcaa atttgtgagg atttttaata
    10621 aagtgcatgt gttatttaat aaagtgacgt ttatagatag aatttataac ataataatag
    10681 atgaatcacg ctgtaattaa tgaagattat ggcgtgattt attattggaa ggaaaattgt
    10741 aggtcctagt acattgattt atgtgccaaa atcgatattg aacccataag tccaatttga
    10801 cagagaacca ttattaaacg tattaaccaa attatattga tagttcgaat ttcactatga
    10861 accattatgt attgtttggt aacaaaaaaa gaactaagag gttgagctct tagttcttaa
    10921 gtaaatgtgt cctccacata aggtcacatt ttttatgtgc aatatattta ttaaactcta
    10981 gttactttac cagattttaa agcacgtgca gaaacccaaa cttttttagg tttaccgtca
    11041 actaggattc taactttttg aaggttagcg ttccatctac gtttagtaga gtttaaagcg
    11101 tgtgaacgtc tgttaccagt cgaagcttta cgacctgtta cgaaacattg tttacccata
    11161 tgagtacctc ctttaataaa ta
//
LOCUS       DAHHSC010000069        11087 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.069, whole genome shotgun sequence.
ACCESSION   DAHHSC010000069 DAHHSC010000000
VERSION     DAHHSC010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 11087)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11087)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11087
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.069"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            complement(211..486)
                     /locus_tag="NBI57_001378"
     CDS             complement(211..486)
                     /locus_tag="NBI57_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaN family protein"
                     /protein_id="HCD1822331.1"
                     /translation="MAKQATMKNAALKQLTKDADEILHLIKVQLDNLTLPSCPLYEEV
                     LDTQMFGLQKEVDFAVKLGLVDREDGKQIMLRLEKELSKLHEAFTLV"
     gene            627..1109
                     /locus_tag="NBI57_001379"
     CDS             627..1109
                     /locus_tag="NBI57_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1822332.1"
                     /translation="MIQIKGAVKFPITLDSTTWIFDDRKVSIEDLEKGVFDGSKPIDF
                     EDNHEWNRAILEGQTNPPTLNSEIKYKKRAMLEGSFVINMTPFFKHSEPHDSATTIRL
                     SNEHDHLDIPIDLLPYLFFQFAKDGKRLYSDNGVDSFIYNQEDGYSYRINHVTHIEVI
                     "
     gene            1111..1278
                     /locus_tag="NBI57_001380"
     CDS             1111..1278
                     /locus_tag="NBI57_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaI family protein"
                     /protein_id="HCD1822333.1"
                     /translation="MRQVQCIICDAKVFIDERTTESKRLKNNPIRTFMCDDCKSRLDT
                     PKQRAQHYPLD"
     gene            complement(1418..3265)
                     /gene="typA"
                     /locus_tag="NBI57_001381"
     CDS             complement(1418..3265)
                     /gene="typA"
                     /locus_tag="NBI57_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HCD1822334.1"
                     /translation="MTNKREDVRNIAIIAHVDHGKTTLVDELLKQSGIFRENEHVDER
                     AMDSNDIERERGITILAKNTAVDYKGTRINILDTPGHADFGGEVERIMKMVDGVVLVV
                     DAYEGTMPQTRFVLKKALEQNLKPVVVVNKIDKPSARPEGVVDEVLDLFIELEANDEQ
                     LEFPVVYASAVNGTASLDPEKQDDNLQSLYETIIDYVPAPIDNSDEPLQFQVALLDYN
                     DYVGRIGIGRVFRGKMRVGDNVSLIKLDGTVKNFRVTKIFGYFGLKRLEIEEAQAGDL
                     IAVSGMEDINVGETVTPHDHQEALPVLRIDEPTLEMTFKVNNSPFAGREGDFVTARQI
                     QERLNQQLETDVSLKVSNTDSPDTWVVAGRGELHLSILIENMRREGYELQVSKPQVII
                     KEIDGVMCEPFERVQCEVPQENAGAVIESLGARKGEMVDMTTTDNGLTRLIFNVPARG
                     MIGYTTEFMSMTRGYGIINHTFEEFRPRIKAQIGGRRNGALISMDQGSASTYAILGLE
                     DRGVNFMEPGTEVYEGMIVGEHNRENDLTVNITKTKHQTNVRSATKDQTQTMNRPRIL
                     TLEEALQFINDDELVEVTPESIRLRKKILNKNVREKEAKRIKQMMQENE"
     gene            3367..3558
                     /locus_tag="NBI57_001382"
     CDS             3367..3558
                     /locus_tag="NBI57_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008530239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaF family protein"
                     /protein_id="HCD1822335.1"
                     /translation="MKQKKSKNIFWVFSILAVVFLVLFSFAVGASNVPMMILTFILLV
                     ATFGIGFTTKKKYRENDWL"
     gene            complement(3712..4539)
                     /locus_tag="NBI57_001383"
     CDS             complement(3712..4539)
                     /locus_tag="NBI57_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase"
                     /protein_id="HCD1822336.1"
                     /translation="MALYGFAQGLIQEAGIRIKQLMEQNLTIETKSNPNDLVTNVDKA
                     TEDFIFDTILETYPNHQVLGEEGHGHDIDTSKGTVWVVDPIDGTLNFVHQQENFAISI
                     GIYIDGKPYAGFVYDVMADVLYHAKVGEGAYRGSQPLKPLNDSNLRQSIIGINPNWLT
                     KPILGEIFKEIVNDSRSARAYGSAALEIVSVATGNLEAYMTPRLQPWDFAGGLVILYE
                     VNGQASNLLGEPLTISGPNSILVGNRGLHQEISNDYLEPYHDTLIQLHEQRFKRKSK"
     gene            4696..5310
                     /locus_tag="NBI57_001384"
     CDS             4696..5310
                     /locus_tag="NBI57_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YktB family protein"
                     /protein_id="HCD1822337.1"
                     /translation="MTKYTFKPKDFKAFNVEGLDARMEALNEYIRPQLHELGEYFSDF
                     FTSQTGETFYPHVAKHARRSVNPPKDTWVAFATSKRGYKMLPHFQIGMFEDQLFVMFG
                     IMHEAKDKATRAKVFERKFKAIQQLPDDYRVCLDHMKPDKPFIKDLTDDDLKEAIQRA
                     INVKKGKFFIARAITPQDKRLKSDKAFIAFLEETFDQFLPFYSA"
     gene            5493..6845
                     /locus_tag="NBI57_001385"
     CDS             5493..6845
                     /locus_tag="NBI57_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HCD1822338.1"
                     /translation="MNNKRHSTNEQLSLDEINNTIKFDHRSSNKQKFLSFLGPGLLVA
                     VGYMDPGNWITSMQGGAQYGYTLLFVILISSLSAMLLQSMTVRLGIATGMDLAQMTRH
                     YLSRPIAIIFWIIAELAIIATDIAEVIGSAIALNLLFNIPLIVGALITVLDVFLLLFI
                     MKYGFRKIEAIVGTLIFTVLFIFIFEVYISSPQLNAVLNGFIPHSEIITNNGILYIAL
                     GIIGATIMPHNLYLHSSIVQSRTYSRHNNEEKAQAIKFATIDSNIQLSIAFVVNCLLL
                     VLGASLFFNSNADDLGGFYDLYHALKTEPVLGATMGAIMSTLFAVALLASGQNSTITG
                     TLAGQIVMEGFLRLHIPNWLRRLITRSLAVIPVIVCLIIFKGNAAKIEQLLVFSQVFL
                     SIALPFCLIPLQLATSNKDLMGPFYNKTWVNIISWTLIIILSILNVYLIVQTFQELQS
                     "
     gene            complement(6924..7343)
                     /locus_tag="NBI57_001386"
     CDS             complement(6924..7343)
                     /locus_tag="NBI57_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HCD1822339.1"
                     /translation="MNRKPELIMAWIANSISIIYLLVMGLSYFSLKSGNASQREELAK
                     QLSQNGGNVSLDMLQTTMGALAIILLISTLYGIFATICIKGRRKLSIILFVIAIIVSL
                     MALNLIAIVLWVIVMIMLISKKESKETTHKDDEYIYH"
     gene            complement(7626..8645)
                     /locus_tag="NBI57_001387"
     CDS             complement(7626..8645)
                     /locus_tag="NBI57_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HCD1822340.1"
                     /translation="MTGEQFTQIKRPVSRLTEKVLGWLCWVMLLVLTVITMFIALVSF
                     SNNTSIANLENTLNNNAFIQQLLAGNGYNTTQFVIWLQNGIWAIIVYFIVCLLISFLA
                     LISMNIRILSGFLFLISAIVTIPLVLLIVTLIIPILFFIIAMMIFIRKDKVEMVAPQY
                     YEEYNGPIYDYREPVYERPQPKDDYYDVPKYEKELDKSNTVYDQEQERDKYDQFPKRA
                     VESEYNHDERTEEEPSVLSRQAKYKQKSTEELGIEDDGYYAEPEVDPKELKAQQKREK
                     AEIKAKKKEKRKAYNQRMKERRKNQPSAVSQRRMNFEERRQIYNNDISEERNSSEVKD
                     KKEQE"
     gene            complement(8719..9792)
                     /locus_tag="NBI57_001388"
     CDS             complement(8719..9792)
                     /locus_tag="NBI57_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein"
                     /protein_id="HCD1822341.1"
                     /translation="MKRFLQLIIGALVVGMLCLTLSHWFKSKEQVHTNQKIYVYNWGE
                     YIDPELIKKFEKETGIQVVYETFDSNEAMEAKIRNGGTHYDVAFPSEYTVQKLKRDHL
                     LLPIDHNKVPNIKNLDSDYMNMSFDKGNKYSLPYFFGTVGILYNKEKYPNESFDSWKS
                     LYNPKFKNQILLVDGAREIIGMSLNKLGYNLNDRNSHHLKEAERDLTKLAPQVRGVVG
                     DEITMMLQQNEGNIAVVWSGVAAPLVQEGDKYNYVIPKEGSNLWFDNMVIPKTAQNKE
                     GAYKFMNFLLDAKNNRQNTEFVGYATPNKAARQLLPKEIKDDHRFYPTKKEQERLEVY
                     KDLGPEVLSEYNENFLNFKMSLK"
     gene            complement(9792..10601)
                     /locus_tag="NBI57_001389"
     CDS             complement(9792..10601)
                     /locus_tag="NBI57_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCD1822342.1"
                     /translation="MKWYGKLYIGILLAILYIPIFFLMFYSFNSAGNMIHFEHFTLEH
                     YQSLFQNDHLMSVIFNTIAVALLAASISTVIGTFGAIAIYYLRNKKFKVTLLTLNNVL
                     MVSSDVVIGASFLIMFTTIGHFTGLGLGFWTVLISHIAFCIPIVVIIVLPQLYEMNNN
                     MLNAARDLGATEPQLLSNIIIPNILPSIIGGFFMALTYSLDDFTVSFFVTGNGFSVLS
                     VEVYAMARKGISMEINAISTLLFAVIVLGILGYYLIQYVINKKKLIKRGVK"
     gene            complement(10607..>11087)
                     /locus_tag="NBI57_001390"
     CDS             complement(10607..>11087)
                     /locus_tag="NBI57_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCD1822343.1"
                     /translation="YAFIGLLSHDGVINQFFHLFNLPSFNLLFTTGAFLVVASYIYIP
                     FMILPIFNSMKAIPNNLLQASSDLGASPFYTFRKVIMPLTKEGVMTGVQVTFIPSLSL
                     FMITRLIAGNKVINIGTAIEEQFLTIQNYGMGSTIAIFLIVFMAFILIITKSSNGRG"
BASE COUNT         3716 a         1927 c         1575 g         3869 t
ORIGIN      
        1 tcacaaatat taacaggtct cttgaggtaa tttatagaat atttgtttac ttaatatcgt
       61 gaattgctaa aacccaataa aagtgactga catgtcatca agaagacaac ataacagcca
      121 ctttaatcag taattaaact ataaatataa aatcggcgca gctcaattgt cgatatctca
      181 cgccatttta atatgcttat atattaaacc ttaaacaagt gtaaaagctt catgtaattt
      241 tgaaagttct ttctcaagac gtaacataat ttgtttgcca tcttcgcggt caactaaacc
      301 taatttaaca gcaaaatcaa cttctttttg aagtccaaac atttgtgtat ctagtacttc
      361 ttcatataat gggcatgaag gtaatgttaa attatctagt tgaactttaa tcagatgcaa
      421 gatttcatca gcatctttag tcaattgttt caaagctgca tttttcattg ttgcttgttt
      481 cgccatgaca cgctccccct ctatataaat tctcaagtct tactaaaagt ttatcttact
      541 aaatattaaa aagcaagtag atttaaaatg tgtcgttagc aatttgtgtt aaaataatgc
      601 tatatacatt gatagggagt gggaaaatga tacaaattaa aggagccgta aagtttccta
      661 taacattaga tagtacgact tggattttcg atgatagaaa agttagcatc gaagatttag
      721 aaaaaggtgt atttgatggt tcaaaaccaa tcgactttga agataatcat gaatggaatc
      781 gtgctatcct agaaggacaa actaatccgc ctactttaaa ttcagaaata aaatataaaa
      841 aacgtgccat gttagaaggt tcatttgtca ttaatatgac accatttttc aaacattccg
      901 aaccacatga ctctgcaact actattcgat tatcaaatga gcatgatcat ttagatatac
      961 cgatagatct attaccctat ttatttttcc aatttgcaaa agacggtaaa cgactttaca
     1021 gcgataatgg tgtagatagt tttatttaca atcaagaaga tggttattca tatcgaatca
     1081 accatgtaac tcatattgag gtgatttaat ttgagacaag tgcaatgcat tatctgtgat
     1141 gcaaaagtat ttatagatga gcgcacaact gagtctaaac gacttaaaaa caatcctatt
     1201 cgaacattta tgtgtgatga ttgtaaaagt cgattagaca cacctaaaca acgtgcgcaa
     1261 cactatccgc tcgattaata tctgaataaa caagttagaa atttgaaaat gtgaaagtaa
     1321 caggaaataa tagcatttaa acactttttc aaatattata tgccaaaaga gaagctaagc
     1381 aactttcatc gctcagcttc tcttttatta tgttaaatta ttcgttttct tgcatcattt
     1441 gtttgatacg ctttgcttct ttttcacgaa catttttgtt taaaattttc tttcttaaac
     1501 gtatactttc tggtgtaacc tcaacaagtt catcatcatt aatgaattgt aacgcttctt
     1561 ccaatgttag aatacgcggt ctattcattg tttgtgtttg gtctttcgtt gcagaacgta
     1621 cgttagtttg atgttttgtt ttagtgatgt taacagttaa atcattttca cgattatgtt
     1681 caccaacaat cataccttca taaacttcag taccaggttc catgaagttt acacctctat
     1741 cttcaagtcc caaaatggca taagtacttg cagaaccttg atccattgaa attaatgcac
     1801 cattacgacg accgccaatt tgtgctttaa tacgtggtct aaattcttca aatgtatggt
     1861 taataatacc gtaacctctt gtcattgaca taaattcagt cgtataacca atcataccac
     1921 gagccggtac attaaagatt aaacgtgtaa gtccattatc agttgtagtc atatcaacca
     1981 tttcaccttt acgtgcacct aatgattcaa taacagcacc tgcattttct tgtggcactt
     2041 cacattgcac acgttcaaat ggttcacaca ttacaccatc tatttcttta ataattactt
     2101 gtggttttga aacttgtaat tcataacctt cacgacgcat attttcaata aggattgata
     2161 aatgcaattc accgcgacca gcaactaccc atgtatctgg agaatctgtg ttagaaactt
     2221 tcaaagatac atctgtttct aattgttgat ttaaacgttc ttgaatttga cgtgctgtta
     2281 caaagtcacc ttcacggcca gcaaatggag aattgttaac tttaaatgtc atttcaagag
     2341 taggctcatc aatacgtaga actggcaatg cttcttgatg gtcatgtggt gttacagttt
     2401 caccaacatt aatgtcttcc atacctgaaa cagcaattaa atctccagct tgtgcttctt
     2461 caatttctaa acgttttaat ccaaagtaac caaagatttt agttacacgg aagtttttca
     2521 ctgtaccgtc taatttaatt agtgatacat tatctccgac acgcatttta cctctgaata
     2581 cacgaccaat accaatacgt ccaacataat cattgtagtc caacaatgct acttggaatt
     2641 gtaatggctc atcactgtta tcaattggag ctggtacata atcaataatt gtttcatata
     2701 atgattgtaa attatcatct tgtttttcag gatctaagct agctgttcca tttactgctg
     2761 aagcataaac aacagggaat tctaattgtt catcgtttgc ttctaattca ataaataaat
     2821 ctaaaacttc atctacaaca ccctctggac gtgctgatgg tttatcaatt ttattaacaa
     2881 caacaacagg tttcaggttt tgttctagcg cttttttaag tacaaaacgt gtttgaggca
     2941 ttgtaccttc atacgcatct actactaaga caaccccatc aaccattttc ataatacgtt
     3001 ctacttctcc accaaagtct gcatgtcctg gtgtatccaa aatattaata cgtgtacctt
     3061 tataatcaac agccgtattt ttggctagaa tcgtaattcc acgctctctt tcgatatcgt
     3121 tagagtccat tgcacgttca tcgacatgtt cattttctct gaatatacca gattgtttta
     3181 acaactcatc tactaaagtt gttttaccat ggtcaacgtg agcaataatt gctatattgc
     3241 ggacatcttc tcttttatta gtcatataaa tagtttcctt ctttcttaag taaaatccca
     3301 cttttaatta caactttttt attatatcat ataattgaga aaagataagc aaaaggtggg
     3361 gataggatga aacaaaaaaa atctaaaaat atcttttggg tattttctat attagctgtt
     3421 gttttcttag tattatttag ttttgctgtt ggtgcatcaa atgtaccaat gatgatttta
     3481 acatttatat tactcgttgc aacctttgga attggattta ctacaaagaa aaaatatcga
     3541 gaaaacgatt ggctataagt tatatcgaca ataacacgcg ttgatttaag accacacatg
     3601 ttcattatga acatttaaat ggttttatat tggcgcgtgt ttttattatt taaggtataa
     3661 aagcgtaata tgttacctaa ttgttaggac tacatattac gcttttggta attattttga
     3721 ttttctttta aatcgttgtt catgtaattg tattaacgta tcatggtagg gctctaaata
     3781 atcattgcta atttcttgat ggagacctcg atttccaact aagattgaat ttggaccact
     3841 aattgttaat ggttctccta gtaaatttga agcttgtcca tttacttcat ataaaataac
     3901 caatccgcca gcaaaatccc atggttgaag tcttggcgtc atatatgctt ctaaattacc
     3961 tgtagcaact gaaacgattt caagcgctgc actaccatat gcccttgcac ttctagaatc
     4021 attaacaatt tctttaaaga tttctcctaa aattggttta gttaaccagt tcggattgat
     4081 cccaataatg ctttgtctta gattagaatc attcaatggt ttcaagggtt ggctacctcg
     4141 atatgcgcct tcccctactt tagcatgata taagacgtca gccataacat catatacaaa
     4201 acctgcataa ggtttaccat cgatataaat accaattgaa attgcgaaat tttcttgttg
     4261 atgaacaaaa ttcaatgtac cgtctattgg gtcaacaacc cataccgtac ctttggaagt
     4321 atcgatgtca tgaccatgcc cttcttcacc taatacttga tgattgggat atgtttctaa
     4381 aattgtatca aaaatgaaat cttctgttgc tttatctaca tttgtaacaa ggtcattcgg
     4441 atttgacttt gtttcaattg ttaaattttg ctccatcaat tgtttaattc taattcctgc
     4501 ttcttgaata agtccttggg caaatccata aagtgccata acatcatctc ctaacatctt
     4561 tattatacat caacatttta taaaaataac ttcttatgat aaaaatgaaa atactaattt
     4621 taatgagagc gtcttaagcc gcaattgata aaacatatgc tacaatattt ttaaacacta
     4681 tacaggaggt actctatgac aaaatataca tttaaaccta aagatttcaa agcgttcaac
     4741 gtagaaggct tagacgcacg aatggaagct ttaaacgaat acatacgacc acaactccat
     4801 gaattaggag aatattttag tgacttcttt acaagtcaaa ctggtgaaac attttatcct
     4861 cacgtagcaa aacatgctag aagaagtgtg aatcctccta aagatacatg ggttgctttt
     4921 gctacaagca aaagaggcta taaaatgtta cctcatttcc aaattggtat gtttgaagat
     4981 caactgtttg ttatgtttgg aatcatgcat gaagcaaaag ataaagcaac acgtgcaaaa
     5041 gtttttgaaa gaaaatttaa agctattcaa caattacctg atgattatcg tgtttgctta
     5101 gatcatatga aacctgataa accatttatt aaagatttaa cggatgatga tttaaaagaa
     5161 gcgatacaaa gagccatcaa tgtgaaaaaa ggtaaattct ttatagcgcg tgcaatcaca
     5221 ccacaagata aaagattaaa aagtgacaaa gcatttattg catttttaga agaaaccttc
     5281 gatcagttct taccatttta ttctgcataa ataactttgt ttaaataata gagcacgtaa
     5341 tcacatccat gatttcgtgc tcttttttct taatattaaa tcgaacgttc aacataataa
     5401 ttcatacttt taaaaaaata aaataaattt aggttgacct aaacatttta ttaggttatt
     5461 atattgtcca taagaagtag aggtgagtca aaatgaataa taaacgacat tcaacaaatg
     5521 aacaattaag tttagacgaa ataaacaata caattaaatt cgatcatcgc agttcaaata
     5581 aacagaaatt tttatcattt cttggacctg ggttattagt cgctgttggt tacatggatc
     5641 ccggaaactg gataacatca atgcaaggtg gcgcccaata tggctatact ttgctattcg
     5701 taattcttat ttcaagttta tcagcaatgt tacttcaaag catgacagtg agattaggaa
     5761 tagcaacagg tatggactta gctcaaatga cacgacatta tttatcaaga cctattgcta
     5821 taatcttttg gatcattgca gaactagcaa ttatcgctac agatattgct gaggttattg
     5881 gtagtgctat tgctcttaat ctcctattta acataccttt aatcgtcggt gcactaataa
     5941 ctgtacttga tgtattttta ctactattta taatgaaata tggttttaga aaaattgaag
     6001 ctattgttgg tacattaatt ttcacagtgt tattcatctt tatatttgaa gtctatattt
     6061 catcaccaca gttgaatgct gtgttaaatg gatttatacc acatagtgaa atcattacaa
     6121 ataacggcat tctctatatt gcattaggta ttattggcgc tacaattatg cctcataatt
     6181 tgtacttaca ttcatcaatt gtacaatcta gaacatactc aagacataac aatgaagaaa
     6241 aggcgcaagc gattaaattt gctacgatag attcgaacat tcagttatca atcgcatttg
     6301 tagtcaattg cttattatta gtgttaggag catcactatt tttcaactcg aacgctgacg
     6361 atttaggtgg tttctatgat ttatatcacg ccttaaaaac tgaacctgta ctaggtgcaa
     6421 caatgggtgc aatcatgagt acattatttg cagttgcatt attagcgtca ggtcaaaatt
     6481 caacgattac tggtacttta gcaggacaaa ttgtaatgga aggattttta agattacaca
     6541 taccaaattg gttaagacgt ttaattacac gttcccttgc tgtcattcct gttatcgtat
     6601 gcttaatcat ttttaaaggt aatgcagcta aaattgagca actacttgta ttctctcaag
     6661 tatttttaag tattgctctg ccattctgtt taataccatt acaattagct actagtaata
     6721 aagacttgat gggacctttc tataataaaa catgggttaa tatcatttca tggacactta
     6781 ttattattct aagtatttta aatgtctatt taatcgttca aacattccaa gaactacaaa
     6841 gttaaaaata aaaccattaa aatgttggtt gtatctaata attaacaact taacatttta
     6901 atggtttttt tgctatgaac atattaatga taaatatact catcgtcctt atgtgtagtt
     6961 tcttttgatt cttttttaga aatcaacata atcatcacga taacccataa gacaattgca
     7021 attaaattaa gagccatcaa acttacaatt atcgcgataa caaaaagtat aatcgataat
     7081 tttctacgcc ctttaataca aattgtcgca aatataccat aaagtgttga aattaataaa
     7141 ataattgcta atgcacccat tgttgtctga agcatatcta aagaaacatt gccaccgttc
     7201 tgagataatt gcttcgctaa ttcttcacgt tgtgatgcat taccactttt taaagaaaaa
     7261 taggatagcc ccataactaa taaataaata atactaatac tatttgcaat ccaagccatg
     7321 attaattcag gttttctatt catcttaaaa tcctttcttt gtttaatatt ttaataagta
     7381 tactacgagc ctatagatat acgcacatta aattacttgt atattaacat atttttcatc
     7441 taatttttat aataaacatc tgaattcaga ttgatgataa tttaggtgta tggtaatgag
     7501 aaatcgtcat ttaaaatttt gacacctaaa cactttaatt aaacgttatc gatgattaac
     7561 ctcatataaa aacccagatt aataagcaaa agacctctac ttactaatct gggtatcatc
     7621 aatatttatt cttgctcttt tttgtcctta acttcacttg aattgcgttc ttcagaaata
     7681 tcattgttgt aaatttgacg tcgctcttca aaattcattc gacgttgact aactgcgcta
     7741 ggttgatttt tcctacgttc tttcattcgt tggttataag ctttacgttt ttcttttttc
     7801 ttagctttaa tttcagcttt ttctcgcttt tgttgtgctt tcaattcttt tggatcaact
     7861 tcaggttctg cataataacc atcatcttca atacctagtt cttcagtact tttttgtttg
     7921 tatttagcct gtctggataa tactgatggt tcttcctcag tgcgctcatc atgattatat
     7981 tcactttcaa ctgcacgttt aggaaattga tcatatttat ctctttcctg ttcttgatca
     8041 tatacagtat ttgatttatc caattctttt tcatatttag gcacatcata ataatcatct
     8101 ttcggttggg ggcgctcata cacaggttca cgataatcat aaatcggtcc gttatactct
     8161 tcataatatt gtggcgcaac catttcaact ttatcttttc ttataaatat catcatcgca
     8221 ataataaaga ataatatcgg aatgattaaa gtaacgataa gtaaaactaa aggaatcgtt
     8281 acaattgctg atattaagaa taagaaacct gacaagattc ttatattcat agatatgaga
     8341 gctaaaaatg aaatcaacaa acaaacaata aagtaaacga taatagccca tataccattt
     8401 tgtaaccata ttacaaattg tgttgtatta tagccatttc cagctaataa ttgctggata
     8461 aatgcattat tgtttaatgt attttcaaga ttagctatcg atgtgttatt actgaatgaa
     8521 acaagtgcta taaacatcgt aatgacagta agcactaata acatcaccca acataaccaa
     8581 cctagaactt tttcagttaa tctacttact ggacgtttaa tttgagtaaa ttgttctcca
     8641 gtcattcgtt acaactcctt atagtactta tcccgttatt ataactaaat atacagtaaa
     8701 taactactat ttatgatttt attttaatga cattttgaaa ttcaaaaagt tttcattgta
     8761 ttcacttaaa acttcaggtc ctaaatcttt ataaacttca aggcgttctt gctctttctt
     8821 agtcggataa aaacgatggt cgtctttaat ctctttaggc aacaattgtc gagcagcctt
     8881 gtttggcgtt gcatagccta cgaattctgt attttgcctg ttatttttag catctaataa
     8941 aaaattcata aatttatatg caccctcttt attttgtgcc gtttttggaa ttaccatatt
     9001 gtcgaaccat aaattcgatc cttctttagg aataacataa ttatatttat ccccttcttg
     9061 cactagaggt gctgcaacac cactccaaac aaccgctatg ttaccttcat tttgttgaag
     9121 catcatggta atttcatcac ctacgacacc tcttacttgt ggtgctagtt tggttaaatc
     9181 tcgctctgct tcttttaaat ggtgcgaatt acggtcatta agattatacc caagtttatt
     9241 caaactcatg cctataatct ctctagcacc gtcaactagt aaaatttggt ttttaaattt
     9301 aggattatac aatgacttcc aactatcaaa tgattcattt ggatactttt ctttattata
     9361 taaaatacct acagttccaa agaaataagg taaagaatat ttattgcctt tatcaaatga
     9421 catattcata taatctgaat ctaaattttt aatattaggt accttattat gatctattgg
     9481 taacaataaa tgatctcttt tcaatttttg aactgtatat tcactaggaa aagcaacatc
     9541 ataatgtgta ccgccattgc gaattttggc ttccatcgct tcatttgaat cgaaagtttc
     9601 ataaacgact tgaatgccag tttctttttc aaatttctta attaactctg gatcaatata
     9661 ttcgccccaa ttgtatacgt aaattttttg atttgtatgc acttgttctt tagatttaaa
     9721 ccaatgactt aaagtaagac aaagcatacc cacaactaat gcacctataa tgagttgtaa
     9781 aaaacgtttc attattttac acctcgcttg attagttttt tcttatttat cacgtattga
     9841 atcaaataat atcctagtat tcctaataca ataacagcaa acaataatgt tgaaatcgca
     9901 ttaatttcca tactaattcc ttttctcgcc atagcataaa cttcaactga taacacacta
     9961 aagccattac cagtaacgaa gaaacttact gtgaaatcgt ctagtgaata agttaaagcc
    10021 ataaagaaac ctcctataat agaaggtaaa atattaggaa taataatgtt gcttaataat
    10081 tgtggttcag tcgctcctaa atctcttgca gcatttaaca tattattatt catttcatac
    10141 agttgtggta agacgataat cacaactata ggtatgcaaa atgcaatatg agatattaga
    10201 actgtccaaa atcctaaacc aagaccagta aaatggccaa tcgttgtaaa cataattaag
    10261 aatgatgcac ctatgacaac gtcggatgat accatcaaga cattattcaa tgttagtaaa
    10321 gttactttaa actttttatt tcttaaataa taaatagcaa tggcaccaaa tgtaccaata
    10381 actgtagaaa ttgaggctgc taaaagtgct acagctatcg tattaaaaat gaccgacatt
    10441 aaatgatcat tttgaaataa tgattgataa tgctctaatg taaaatgttc aaagtgaatc
    10501 atattaccag ccgaattgaa tgaatagaac attaaaaaga atattgggat gtataaaatc
    10561 gctaaaagta tcccgatata cagctttcca taccatttca tatgattcac cctctcccat
    10621 tagatgattt tgtaatgatt aaaatgaatg ccataaatac aattaagaat atagctatag
    10681 ttgatcccat accataattt tgaattgtta aaaattgttc ctctattgcc gtacctatat
    10741 ttatgacttt attacctgca attaatcttg taatcataaa taatgaaagt gatggaataa
    10801 aggttacttg aaccccagtc ataacacctt cttttgttaa cggcatgatt acttttctaa
    10861 aagtatagaa aggactggca cctaaatcac ttgaggcctg caataaatta ttaggaattg
    10921 ctttcatgct attaaatata ggtaaaatca taaatggtat ataaatgtaa cttgccacta
    10981 ctaaaaacgc accagttgta aataacaaat tgaatgatgg taaattaaat aagtggaaaa
    11041 attgattaat cacgccatca tgacttaata aacctataaa agcatat
//
LOCUS       DAHHSC010000070        10930 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.070, whole genome shotgun sequence.
ACCESSION   DAHHSC010000070 DAHHSC010000000
VERSION     DAHHSC010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 10930)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10930)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10930
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.070"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            complement(<1..721)
                     /locus_tag="NBI57_001391"
     CDS             complement(<1..721)
                     /locus_tag="NBI57_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein"
                     /protein_id="HCD1822344.1"
                     /translation="MKAHKIFWLNLAAIIIISIVVSGGMFLAMKWEQIHLKDGLKKVL
                     STYPIKNLETLYEIDGHDNPHYENNDQDTWYIESSYSVVGSDELLKEDRMLLKVDKNT
                     HKITGEYDTTTNDRKDATDSTYKSYPVKVVNNKIVFTKDVKDPALKQKIENNQFLIQS
                     GDLTSILNSNDLKVTHDPTTDYYNLSGKLSNDNPNVKQLKRRYNIPSNASTKVELKGM
                     SDLKGNNHQDQKLYFYFSSPGK"
     gene            complement(910..1536)
                     /locus_tag="NBI57_001392"
     CDS             complement(910..1536)
                     /locus_tag="NBI57_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HCD1822345.1"
                     /translation="MAKVLYITAHPFNELVSNSMAAGKAFIETYQQQHPDDEVKHIDL
                     FETYIPVIDKDVLTGWGKMSNGETLTDDEQMKVSRLSDILEEFLSADKYVFVTPMWNL
                     SFPPVVKAYIDAISIAGKTFKYSAEGPQGLLTDKKVLHIQSRGGYYTEGPAADFEMGD
                     RYLRTIMTFLGVPSYETIIIEGHNAEPHKTEEIKATSINNAEKLATTF"
     gene            1745..2323
                     /locus_tag="NBI57_001393"
     CDS             1745..2323
                     /locus_tag="NBI57_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HCD1822346.1"
                     /translation="MTKRPKRILATIIIFLSLLFTIIYIDDIQKWFNQYTDKLTQNHK
                     GQGHSKWEDFFRGSRITETFGKYQHSPFDGKHYGIDFALPKGTPIKAPTNGKVTRIFN
                     NELGGKVLQIAEDNGEYHQWYLHLDKYNVKVGDRVKAGDIIAYSGNTGKQTTGAHLHF
                     QRMKGGVGNAYAEDPKPFIEQLPDGERSLYDL"
     gene            2707..3804
                     /gene="ugpC"
                     /locus_tag="NBI57_001394"
     CDS             2707..3804
                     /gene="ugpC"
                     /locus_tag="NBI57_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HCD1822347.1"
                     /translation="MAELKLEHIKKTYDNNNTVVKDFNLHITDKEFIVFVGPSGCGKS
                     TTLRMVAGLESITSGDFYIDGERMNDVEPKNRDIAMVFQNYALYPHMTVFENMAFGLK
                     LRKVNKKEIEQKVNEAAEILGLTEYLGRKPKALSGGQRQRVALGRAIVRDAKVFLMDE
                     PLSNLDAKLRVQMRTEILKLHKRLNTTTIYVTHDQTEALTMASRIVVLKDGDIMQVGT
                     PREIYDAPNCIFVAQFIGSPAMNMLNATVEMDGLKVGTHHFKLHNKKFEKLKAAGYLD
                     KEIILGIRAEDIHEEPIFIQTSPETQFESEVVVSELLGSEIMVHSTFQGMELISKLDS
                     RTQVMTNDKITLAFDMNKCHFFDEKTGNRIV"
     gene            3817..5088
                     /locus_tag="NBI57_001395"
     CDS             3817..5088
                     /locus_tag="NBI57_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HCD1822348.1"
                     /translation="MSKILKCITLAVVMLLIVTACGPNRSKEDIDKALNKDNSKDKPN
                     QLTMWVDGDKQMAFYKKITDQYTKKTGIKVKLVNIGQNDQLENISLDAPAGKGPDIFF
                     LAHDNTGSAYLQGLAAEIKLSKDELKGFNKQALKAMNYDNKQLALPAIVETTALFYNK
                     KLVKNAPQTLEEVEANAAKLTDSKKKQYGMLFDAKNFYFNYPFLFGNDDYIFKKNGSE
                     YDIHQLGLNSKHVVKNAERLQKWYDKGYLPKAATHDVMIGLFKEGKVGQFVTGPWNIN
                     EYQETFGKDLGVTTLPTDGGKPMKPFLGVRGWYLSEYSKHKYWAKDLMLYITSKDTLQ
                     KYTDEMSEITGRVDVKSSNPNLKVFEKQARHAEPMPNIPEMRQVWEPMGNASIFISNG
                     KNPKQALDEATNDITQNIKILHPSQNDKKGD"
     gene            5091..6359
                     /locus_tag="NBI57_001396"
     CDS             5091..6359
                     /locus_tag="NBI57_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HCD1822349.1"
                     /translation="MTKRNPKLAALLSVIPGLGQFYNKRPIKGTIFFIFFISFISVFY
                     SFLNIGFWGLFTLGTVPKLDDSRVLLAQGIISILLVAFAIMLYVINILDAYRNAERFN
                     RNEEIKDPKARMVATWDKTFPYLLISPGTFLLIFVVVFPLIFMFGVAFTNYNLYNAPP
                     RHTLEWVGLDNFKTLFTIGVWRKTFFSVITWTLVWTLVATTLQIALGLFLAIIVNHPV
                     VKGKKFIRTVLILPWAVPSFVTILIFVALFNDEFGAINNDILQPLLGVAPAWLSDPFW
                     AKVALIGIQVWLGFPFVFALFTGVLQSISSDWYEAADMDGASSWQKFRNITFPHVIYA
                     TAPLLIMQYAGNFNNFNLIYLFNKGGPPVSGQNAGSTDILISWVYNLTFEFNNFNMGA
                     VVSLIIGFIVAIVAFIQFRRTSTFKDEGGL"
     gene            6361..7200
                     /locus_tag="NBI57_001397"
     CDS             6361..7200
                     /locus_tag="NBI57_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HCD1822350.1"
                     /translation="MTKKKNILKAIGIYSFIAMMFVIILYPLLWTFGISLNPGTNLYG
                     AKMIPDNATFKNYAFLLFDDSSQYLTWYKNTLIVASANALFSVIFVTLTAYAFSRYRF
                     VGRKYGLITFLILQMFPVLMAMVAIYILLNTIGLLDSLFGLTLVYIGGSIPMNAFLVK
                     GYFDTIPKELDESAKIDGAGHMRIFLQIMLPLAKPILAVVALFNFMGPFMDFILPKIL
                     LRSPEKFTLAVGLFNFINDKYANNFTVFAAGAIMIAVPIAIVFLFLQRYLVSGLTTGA
                     TKG"
     gene            7274..8350
                     /locus_tag="NBI57_001398"
     CDS             7274..8350
                     /locus_tag="NBI57_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCD1822351.1"
                     /translation="MTIKVGIIGCGGIANGKHMPSLQKVENVEMIAFCDVDISKAASA
                     AEAYGTDNAKVYDDYKALLKDDTIDVIHVCTPNDSHCEITVAGLHAGKHVMCEKPMAK
                     TTAEAQKMIDTAKSTGKKLTIGYQNRFRPDSQFLHQAAQRGDLGDIYFGKAHAIRRRA
                     VPTWGVFLNEEAQGGGPLIDIGTHALDLTLWMMDNYEPESVMGSTFHKLNKQHDAANA
                     WGSWNPDEFTVEDSAFGFIKMKNGATIILESAWAINSLEVDEAKCSLSGTKAGADMKD
                     GLRIHGEDMGTLYTKHVELENKGVDFYEGNEVDEAEEEAKAWIDAVVNDTEPVVKPEQ
                     AMVVTKILEAIYQSAKSGKAIYFE"
     gene            8375..9415
                     /locus_tag="NBI57_001399"
     CDS             8375..9415
                     /locus_tag="NBI57_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCD1822352.1"
                     /translation="MTKLKVGVIGVGGIAQDRHIPALLKLKDTVSLVAVQDINTVQMI
                     DVAKRFNIPQAVETPSELFKLVDAVVICTPNKFHADLSIEALNHGVHVLCEKPMAMTT
                     EECDRMIEAANKNHKLLTVAYHYRHTDVAMTAKKAIEAGVVGKPLVARVQAMRRRKVP
                     GWGVFTNKALQGGGSLIDYGCHLLDLSLWLLGKDMVPHEVLGKTYNQLSKQPNQINDW
                     GTFDHTKFDVDDHVTSYMTFANRASMQFECSWSANIKEDKVHVSLSGEDGGINLFPFE
                     IYEPRFGTIFESKANVEHNEDIAGERQARNFVNACLGIEEIVVKPEEARNVNALIEAI
                     YRSDLDNKSIQL"
     gene            9470..10438
                     /locus_tag="NBI57_001400"
     CDS             9470..10438
                     /locus_tag="NBI57_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HCD1822353.1"
                     /translation="MKIGVFSVLFYDKNFEDMLDYVAESGLDMIEVGTGGNPGDKFCK
                     LDELLENEDKRQAFMKSITDRGLQISGFSCHNNPISPDPIEAKEADETLRKTIRLANL
                     LDVPVVNTFSGIAGSDDTAKKPNWPVTPWPTAYSEIYDYQWNEKLIPYWQDLAEFAKE
                     QDVKIAIELHAGFLVHTPYTMLKLREATNEYIGANLDPSHLWWQGIDPIAAIRILGQA
                     NAIHHFHAKDTYINQENVNMYGLTDMQPYGNVATRAWTFRTVGYGHSPYVWADIISQL
                     IINGYDYVLSIEHEDPIMSVEEGFQKACQTLKSVNIYDKPADMWWA"
     gene            complement(10799..>10930)
                     /locus_tag="NBI57_001401"
     CDS             complement(10799..>10930)
                     /locus_tag="NBI57_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002454321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1822354.1"
                     /translation="LNIIPELIGVLLLTHATYTTILLVPYAYFLYVRIKQEEKLMNI"
BASE COUNT         3685 a         1683 c         2115 g         3447 t
ORIGIN      
        1 tttttccagg acttgaaaaa taaaaataaa gtttctgatc ttgatgatta ttgcctttta
       61 aatcactcat tccctttaat tccacctttg ttgatgcatt actgggaata ttatatctac
      121 gttttaattg tttaacgttt ggattatcat tcgacaactt accagataaa ttataataat
      181 cagtggtagg atcatgtgtg actttcaaat cattgctatt taaaatgctt gttaaatcac
      241 cactttgaat caaaaattga ttgttttcga ttttttgttt cagcgcggga tcttttacgt
      301 cttttgtgaa aacgatttta ttattaacta cttttactgg ataacttttg tatgtcgagt
      361 cagtagcatc ttttctatcg tttgtagttg tgtcatattc accagttatt ttatgtgtgt
      421 tcttatctac ctttaacaac atacggtctt cttttaaaag ctcatctgat ccaacaactg
      481 aataagagga ttctatatac catgtgtctt gatcattatt ttcataatgg ggattatcgt
      541 gaccatcaat ttcataaagc gtttctaagt ttttaatagg atacgtactt agtacttttt
      601 taagaccatc tttcaaatga atttgttccc acttcattgc caaaaacata ccgccactga
      661 ctacaattga aataataata attgctgcta agtttaacca gaaaatttta tgtgctttca
      721 tacattccca ccgtttctca aaatacttca ttaacactat aataatatat tttgaaaaat
      781 atttacatca gtattaaagt gaatatcaaa ttttaaattt atgaaaataa tagatattta
      841 taaaaagcgg aaaagagata caataaaaaa ctgcatgacg ttgaggagtc acacagtgta
      901 attaaaaatt taaaaagttg ttgctaattt ttcagcatta ttaatactag ttgctttaat
      961 ttcttcagtt ttatgcggtt cggcattgtg tccttcaata atgattgttt catatgatgg
     1021 cacacctaag aatgtcataa ttgttcttaa ataacggtca cccatttcaa aatcagcagc
     1081 aggtccttca gtataatatc caccacgtga ttgaatgtgt aatacttttt tgtcagttag
     1141 taaaccttgt ggtccttcag cagaatattt aaaagtttta cctgcaattg aaatagcatc
     1201 aatatatgct ttaactacag gtgggaaaga aaggttccac ataggcgtta caaatacata
     1261 tttatctgca cttaaaaatt cttctaaaat gtcactcaat cttgaaactt tcatttgttc
     1321 atcatcagtt aacgtttcgc cattactcat ttttccccaa ccagttaata catctttgtc
     1381 aataactgga atataagttt caaataaatc aatatgtttc acttcatcat caggatgttg
     1441 ttgttgatat gtttcgataa atgctttacc agccgccata gaatttgata ccagttcatt
     1501 aaaagggtgt gctgtaatat ataatacttt tgccatttga aaattctcct ctgtttctgt
     1561 tattttctta agtataatta ttatactcga tataaaattt aatatcaatc aaaatattca
     1621 aattaccatc attttcttca tctatatttg gcagtactac taaagtatga gtgcatttaa
     1681 ttatgaaata gttgatttag aatatatact taatacccaa aatatatgaa ggatggatgc
     1741 cactatgaca aagcgaccaa aacgtatttt ggcaacaatt atcatttttc tttcactatt
     1801 atttacgatt atttatatag atgacattca aaaatggttt aaccaatata ccgataaatt
     1861 gacacaaaat cataaaggac aaggacactc aaaatgggaa gactttttta gagggagtag
     1921 gattactgag acttttggca aatatcaaca ttcaccattt gatggtaagc attatggcat
     1981 tgattttgca ttgccaaaag gtacaccaat taaagcgccg acgaatggta aagtaacacg
     2041 tatctttaat aatgaattag gcggcaaggt attacagatt gccgaagaca atggagaata
     2101 tcaccagtgg tatctacact tagacaaata taatgtcaaa gtaggtgatc gagttaaagc
     2161 aggtgatatt attgcatatt caggcaatac aggtaaacaa acgacaggcg cacatttaca
     2221 ttttcaaaga atgaagggtg gcgtaggtaa tgcatatgca gaagatccta aaccgtttat
     2281 cgagcagtta cctgatgggg aacgtagcct atatgatttg tagttataga agagtgcccg
     2341 cagtctaaaa aattaagcaa tcattgtgtg agtatgatac ttacataatg gttgcttttt
     2401 tcaatgaaaa tcgtaatgct aagtcatact tgtttgattt agatattact taaaatgtaa
     2461 gacaaggttg ttagcattgg cagtgaaata tcgcacataa aaaaacatta ttgtcacact
     2521 agaaaatagt tgtgtactat atcaattttc tgtataaaag tttaattctg acagtaatgt
     2581 aaacgtttac aatttatgat tgacattaat aatgactgaa tatatgattt atgtaagtat
     2641 ttgtgcaaac gttttcacaa agtgtattgc acaatcaaac tgtaaacaaa gtatgggagg
     2701 cataacatgg cagaactaaa gttagagcat attaaaaaga cgtatgataa caacaatact
     2761 gtagtgaaag attttaatct acatattact gacaaagaat tcattgtatt tgttggacca
     2821 tcgggatgtg gtaaatcaac aacattacga atggttgctg gactagagtc tatcacatct
     2881 ggagattttt atattgatgg ggaacgcatg aacgatgttg aaccaaagaa tagagatatt
     2941 gcgatggtat ttcaaaacta tgcattatat ccacatatga ctgtatttga aaatatggca
     3001 tttgggctaa agctacgtaa agtaaataaa aaagagattg aacaaaaagt caatgaagca
     3061 gctgaaatat taggattaac tgagtatctt ggtcgtaaac caaaagcgtt atctggtgga
     3121 cagcgtcaac gtgttgcttt gggcagagct attgttaggg atgcgaaagt ctttttaatg
     3181 gatgaaccat tatcgaatct tgatgcgaag cttcgagtac aaatgcgcac agaaatattg
     3241 aaattacata agcgacttaa tactacgaca atttatgtta cacatgatca aactgaagca
     3301 ttgacgatgg ctagtcgaat tgttgttttg aaagatggcg acattatgca agtcggcaca
     3361 cctagagaaa tatatgatgc ccctaattgc atatttgtgg cgcaatttat cggctcacca
     3421 gcaatgaata tgttgaatgc tacagttgaa atggacggat tgaaggtagg aacacatcat
     3481 tttaaattac ataataaaaa atttgaaaag ttaaaagctg ctggctactt agacaaggaa
     3541 attattttag gtattcgagc tgaagacatt catgaagaac caatatttat tcaaacttct
     3601 ccagagacac aatttgaatc tgaagtagtt gtatccgagt tgttaggttc agaaatcatg
     3661 gtacatagta cattccaagg aatggaattg atttctaaat tagattcaag aacccaagtg
     3721 atgacgaacg acaagattac actagcattt gatatgaata agtgtcactt ttttgatgaa
     3781 aaaacaggaa atcgtatcgt ctaaggggga gtattcatgt ctaaaatttt aaaatgtatc
     3841 acgttagccg tggtaatgtt attaatcgta actgcatgtg gccctaatcg ttcgaaagaa
     3901 gatattgata aagcattgaa taaagataat tctaaagaca agcctaacca acttacgatg
     3961 tgggtggatg gcgacaagca aatggcgttt tataaaaaaa ttacggatca atatactaaa
     4021 aaaactggca tcaaagtaaa gcttgtaaat attggtcaaa atgatcaact agaaaatatt
     4081 tcgctagacg ctcctgcagg aaaaggtcca gatatctttt tcttagcaca tgataatact
     4141 ggaagtgcct atctacaagg cttagctgct gaaatcaaat tatcaaaaga tgagttgaaa
     4201 ggtttcaata agcaagcact taaagcgatg aattatgaca ataagcaact agcattgcca
     4261 gctatcgtag aaacaaccgc acttttctat aataaaaaat tagtgaaaaa tgcaccgcaa
     4321 acgttagaag aagttgaagc taatgctgcc aagctaactg atagtaaaaa gaaacaatac
     4381 ggtatgttat ttgatgctaa aaatttctat tttaattatc cgtttttatt cggcaatgat
     4441 gattatattt tcaagaaaaa tggcagtgaa tatgatattc atcagctagg actaaattca
     4501 aaacatgtcg tcaaaaatgc tgaacgatta caaaaatggt acgacaaagg gtatcttcct
     4561 aaggcagcaa cacatgatgt catgattggt ctttttaaag aaggaaaagt aggacaattt
     4621 gtcactggac cgtggaacat taatgaatat caagaaacgt ttggtaaaga tttaggagta
     4681 acaacattac ctacagatgg tggcaaacct atgaaaccat ttctcggtgt acgtggttgg
     4741 tatttatctg aatatagtaa acataagtat tgggctaaag atttaatgct gtatatcact
     4801 agtaaagata cattacaaaa atatacagat gaaatgagcg aaattactgg acgtgttgac
     4861 gtgaaatcat ctaatccaaa tttaaaagtg tttgaaaagc aagcacgtca tgctgaaccg
     4921 atgcctaata ttcctgaaat gcgacaagtt tgggaaccga tgggcaatgc aagcatattt
     4981 atctcaaatg gtaagaatcc taaacaagcg ttagatgagg cgacgaatga tataacgcaa
     5041 aatattaaga ttcttcatcc atcacaaaat gataagaaag gagattagtt atgacgaaac
     5101 gtaaccctaa attagcggca ttattatctg ttatacctgg tttgggacag ttttataata
     5161 aaagacccat taaaggtacg atatttttta tctttttcat cagttttatt tctgtatttt
     5221 atagcttttt aaatattggt ttttggggat tgttcacatt agggacagta cctaagttag
     5281 acgattctcg tgtcttactt gcacaaggta ttatttctat cttactcgtt gctttcgcaa
     5341 tcatgctata tgtcattaat attttagatg catatcgtaa tgctgaacga tttaatcgca
     5401 atgaggaaat aaaggatccg aaggcgcgta tggtggcaac atgggacaag acgttcccat
     5461 acttactaat ctcaccaggt acattcttat tgatatttgt agttgtattt ccattaatat
     5521 ttatgtttgg agtagcattt acaaactaca atttatacaa cgcgcctccg agacacacat
     5581 tagaatgggt tggtttagat aactttaaaa cgttattcac aattggcgtt tggcgcaaaa
     5641 catttttcag tgttattact tggacattag tatggacgct tgttgcaacg acacttcaaa
     5701 ttgcattagg gctgtttttg gcaattattg taaatcaccc tgtcgtcaaa ggtaagaaat
     5761 ttatccgtac tgtgttaatc ctaccttggg ctgtaccatc atttgtgaca attttaatat
     5821 ttgtagcgtt atttaatgat gaatttggtg cgataaataa tgatattttg caacctttat
     5881 taggtgtagc accagcatgg ttaagtgatc cgttttgggc aaaagtggca ttaatcggca
     5941 ttcaagtatg gcttggattc ccatttgtct ttgcactgtt cactggagta ctgcaaagta
     6001 tttcatcaga ttggtacgaa gcagcagata tggatggtgc gtctagttgg caaaagttta
     6061 gaaacatcac attcccgcat gtcatttacg ccacagcgcc attgttaatt atgcaatatg
     6121 caggtaattt caataatttt aatcttattt atctatttaa taaaggcggt ccaccagtgt
     6181 cagggcagaa tgctggtagt acagatatct tgatatcttg ggtgtataat ctgacatttg
     6241 agtttaacaa cttcaacatg ggtgcagttg tgtcattaat tattggattt attgttgcta
     6301 ttgtcgcatt tattcaattc agacgtacaa gtacgtttaa agatgaggga ggtttataag
     6361 atgacaaaga agaaaaacat attaaaagca atcggtattt acagttttat agcgatgatg
     6421 tttgtcatca ttttatatcc actactgtgg acatttggca tttcccttaa tccaggtacg
     6481 aacttgtatg gtgccaaaat gataccagac aatgcaacat ttaaaaatta tgcattctta
     6541 ctattcgatg acagtagtca atacctgact tggtataaaa atacgcttat cgtagcatct
     6601 gcaaatgcac tgtttagtgt gatatttgtc acgttaacag catatgcttt ttctagatat
     6661 cgctttgttg gtcgtaaata cgggctgatt acatttttga ttttacaaat gttccctgta
     6721 ttaatggcaa tggtcgcaat ctatattttg ctaaatacaa ttggtttatt agattcttta
     6781 tttggactaa cactggtata tattggtgga tcaataccga tgaatgcctt tttagtgaaa
     6841 ggttacttcg atacgattcc aaaagaactt gatgaatctg ccaaaattga tggtgcaggg
     6901 catatgcgta ttttcttaca aattatgctt ccattagcta agccgatttt agcagttgtt
     6961 gctttgttca attttatggg accatttatg gactttatat tacctaaaat actattaaga
     7021 agtcctgaaa aattcacatt agcagttgga ttgttcaact ttattaatga taagtatgca
     7081 aataatttca cagtgtttgc agcaggggca attatgattg cagtacctat agcaatcgta
     7141 ttcttgttct tgcaacgcta tttagtatca ggtttaacaa caggtgcgac aaaaggttag
     7201 tttgaaatta ggagtggggc agaattgata aagaaccact aatgacgata aagattaaaa
     7261 ggaggacgtt atgatgacga ttaaagttgg aatcattggg tgtggtggta ttgcgaatgg
     7321 caagcacatg ccaagtttac aaaaagttga aaatgttgaa atgatcgcat tttgtgacgt
     7381 agacatttcg aaagcagcga gtgcggcaga agcatacgga actgacaatg caaaggttta
     7441 tgatgattac aaagcattgt taaaagatga cacgattgat gttatccatg tttgtacgcc
     7501 aaatgactcg cattgtgaaa ttactgtagc agggttgcat gccggtaagc atgtgatgtg
     7561 tgaaaaacca atggctaaaa cgacagcaga agctcaaaaa atgatagata cagctaaatc
     7621 aacaggtaaa aaattaacaa taggttatca aaatcgcttc agaccagata gtcaattttt
     7681 acatcaagca gcgcaacgtg gcgacttagg agacatttac ttcggaaagg cacatgccat
     7741 tcgtcgtcgt gcagtaccga catggggtgt ctttctaaac gaagaagccc aaggtggagg
     7801 acctttaatc gatattggta cgcacgcatt agatttaacg ttatggatga tggataatta
     7861 tgaaccagaa tcagtgatgg gttcaacatt ccataaatta aataagcagc atgatgcggc
     7921 aaacgcttgg ggttcatgga atccagatga atttacagtt gaagactctg cgtttggctt
     7981 tattaaaatg aagaacggag cgacgatcat tttagaatcc gcttgggcga ttaattcttt
     8041 agaagtggat gaggcaaaat gttcattatc aggaacgaaa gcaggtgctg atatgaaaga
     8101 tggtctacgt attcatggtg aagatatggg cacactttat accaaacacg ttgaattgga
     8161 aaacaaaggc gtcgactttt atgaaggtaa tgaagtggat gaagctgaag aagaagcaaa
     8221 agcttggatt gatgcagttg taaatgatac tgaaccagtt gtgaaaccgg aacaagcaat
     8281 ggtagttaca aaaattcttg aagcgattta tcagtctgca aaatcaggca aagcaattta
     8341 ctttgaataa catcatacgg taaggaggca catcatgaca aaattaaaag ttggtgtgat
     8401 aggtgttggt ggcattgcac aagaccgtca tattccagca ttgctgaaac tcaaagacac
     8461 agtctcatta gttgcagtac aagatattaa tacagtgcag atgattgatg ttgcgaagcg
     8521 ctttaatata cctcaggcag ttgagacacc tagcgagctg tttaaacttg ttgatgcggt
     8581 ggtcatttgt acacccaata aattccatgc tgatctttct atagaagcat tgaaccatgg
     8641 tgtccatgtc ttatgcgaaa agccaatggc aatgacgacg gaagagtgtg atcgcatgat
     8701 tgaagcggct aataaaaatc acaaattatt aactgtcgct tatcattatc gtcacacaga
     8761 tgtcgctatg actgctaaaa aagcaattga agcaggtgtg gttggtaaac cattagtagc
     8821 acgtgtacaa gcgatgcgta ggcgtaaagt acctgggtgg ggcgttttta ccaataaagc
     8881 gttgcaaggt ggcggtagtt taatcgatta tggttgccac ttgttagact tatctttgtg
     8941 gctattaggt aaagatatgg tgccgcatga agtgctagga aaaacatata atcaattgag
     9001 caaacaaccg aatcaaatta atgattgggg aacatttgat cacactaaat ttgatgtcga
     9061 tgatcatgtt actagttata tgacatttgc caatcgagca agcatgcagt ttgaatgttc
     9121 gtggtctgca aatataaagg aagataaggt acacgttagt ttatcaggag aagatggcgg
     9181 tatcaattta tttccatttg aaatatatga gccccgcttt ggaactattt ttgaaagcaa
     9241 agctaatgtt gagcataacg aagacattgc tggtgagaga caggcgcgta actttgtcaa
     9301 tgcgtgttta ggtatagaag agattgtggt gaaaccggaa gaagcacgca atgtaaatgc
     9361 ccttatagaa gcgatttatc gtagcgatct tgataacaag agcatacaac tttaatgatt
     9421 attatatata atacaaaatt ctcaatataa aaagaaggag tgcttttcaa tgaaaatagg
     9481 tgtattttca gtattatttt acgataaaaa ttttgaagat atgttagatt atgtcgcaga
     9541 atctggattg gatatgattg aagttggaac aggtggtaac ccaggagata aattttgtaa
     9601 gttagatgag ttgttagaaa atgaagacaa gcgacaagca tttatgaagt caatcacaga
     9661 cagaggctta caaataagtg gtttcagttg tcataacaat ccaatttctc cagatccgat
     9721 agaagcgaaa gaagccgatg aaacgttacg taaaacaatc cgtttagcaa atctattaga
     9781 cgtgccagtt gttaatacat tttctggcat tgcagggtca gatgataccg ctaaaaagcc
     9841 taattggcct gttacacctt ggccaacagc ctactctgaa atttatgatt atcagtggaa
     9901 tgaaaagttg ataccatatt ggcaagattt agctgagttt gcaaaagagc aagatgtaaa
     9961 aattgccata gagttacatg caggattttt agttcataca ccatatacga tgttaaagtt
    10021 acgtgaggct acaaatgaat atatcggtgc taacttagat cctagtcatt tatggtggca
    10081 aggtattgat ccaattgctg cgattcgcat attaggccaa gcaaatgcaa ttcatcactt
    10141 ccatgctaaa gatacgtata ttaatcaaga aaatgtaaat atgtatggtc taactgatat
    10201 gcaaccatat ggtaacgttg cgacaagagc atggacattc cgtacagttg gttatggaca
    10261 tagtccatat gtatgggcag atatcataag tcaacttatt attaatggat atgattatgt
    10321 attaagtatt gaacatgaag atcctattat gtcagtagaa gaaggtttcc aaaaagcttg
    10381 tcaaactttg aaatctgtta atatttacga caagccagca gacatgtggt gggcataata
    10441 cgaactcgag gttagtctga agtttgtctg aagtaagact ggtggcagtg ttgaatgaat
    10501 gcatatgtcg ccaagccatt gccaaaaatt tcacacctta aatcatgtca ttgtttgtaa
    10561 agaaggtgta ctttatataa gtgtttagcg atggtcatac ccattcacag taacaatcct
    10621 caccattgaa aagagtatat aaccttttcg atagtgaggt atatgataat aaaaaaagcc
    10681 tgttgtcaca atggtcatag acacgacata ctttaaaggc ttctgaatat aatatttcag
    10741 aacgcacttt aaagatggac gtcgatgtcg actaatgtga tgacaggctt tcatcttttt
    10801 aaatattcat taatttctct tcttgtttaa tacgtacata taagaaatac gcatacggta
    10861 ctaataaaat agttgtatat gttgcgtgtg ttaataataa tacaccgatt aattcaggaa
    10921 tgatgtttaa
//
LOCUS       DAHHSC010000071        10891 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.071, whole genome shotgun sequence.
ACCESSION   DAHHSC010000071 DAHHSC010000000
VERSION     DAHHSC010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 10891)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10891)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10891
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.071"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            89..805
                     /locus_tag="NBI57_001402"
     CDS             89..805
                     /locus_tag="NBI57_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCD1822355.1"
                     /translation="MGRKWNNIKEKKAQKDKNTSRIYAKFGKEIYVAAKSGEPNPESN
                     QALRLVLERAKTYSVPNHIIEKAIDKAKGAGDENFDHLRYEGFGPSGSMLIVDALTNN
                     VNRTASDVRAAFGKNGGNMGVSGSVAYMFDHVATFGIEGKSVDEILETLMEQDVDVND
                     VIDDNGLTIVYAEPDQFAVVQDALRAAGVEEFKVAEFEMLPQTDIELSEADQVTFEKL
                     IDALEDLEDVQNVFHNVDLK"
     gene            805..1278
                     /locus_tag="NBI57_001403"
     CDS             805..1278
                     /locus_tag="NBI57_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HCD1822356.1"
                     /translation="MKSAEQWIDELQLESHPEGGFYRETIREVLKDGRRAPFSSIYFL
                     LTDDNISHFHRIDADEVWYYHAGDSLTIHMINPDGEYTTATLGTDIQNGDVLQYVVPK
                     GTIFASSIEISNTYSLVGCMCQPAFEFKQFELFKQSELITQYPHLKSVIEKYALK"
     gene            1370..1486
                     /locus_tag="NBI57_001404"
     CDS             1370..1486
                     /locus_tag="NBI57_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1822357.1"
                     /translation="MLQLFLMKIALNDFENDKSVLFIFLKNHFYEEMCVLSN"
     gene            1631..2275
                     /locus_tag="NBI57_001405"
     CDS             1631..2275
                     /locus_tag="NBI57_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HCD1822358.1"
                     /translation="MKVKYIDKRHWRRLIDREYTEVKVNNNRFKGIIGLVTMKKVRDP
                     LEVTVVGQNIIVADDNYKWLQILPEKKRYSITVMFDNKGNPLEYYFDINIKNITQKGN
                     ARTVDLCLDVLALPSGEYELVDEDDLMFALESEQITKKQFHEAYMIAHQIMAELENDF
                     KGFQKKIMYCFNKINAKAQKNHQKPQNKTNIEKSKQIKPKQYNQTKNHQQQKKN"
     gene            2391..3257
                     /locus_tag="NBI57_001406"
     CDS             2391..3257
                     /locus_tag="NBI57_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCD1822359.1"
                     /translation="MKIEDYRLLITLDETKTLRKAAEILYISQPAVTQRLKAIENAFG
                     VDIFIRTKKQLITTTEGTMIIEHARDMLKRERLFFDKMQAHIGEVNGTISIGCSSLIG
                     QTLLPEVLSLYNAQFPNVEIQVQVGSTEQIKANHRDYHVMITRGNKVMNLANTHLFND
                     DHYFIFPKNRRDDVTKLPFIEFQADPIYINQIKQWYNDNLEQDYHATITVDQVATCKE
                     MLISGVGVTILPEIMMKNISKEQFEFEKVEIDNEPLIRSTFMSYDPSMLQLPQVDSFV
                     NLMASFVEQPKA"
     gene            3389..4609
                     /locus_tag="NBI57_001407"
     CDS             3389..4609
                     /locus_tag="NBI57_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter"
                     /protein_id="HCD1822360.1"
                     /translation="MDMFAALLQIKNYKLFVANMFLLGMGIAVTVPYLVLFATKDLGM
                     TTNQYGLLLASAAISQFTVNSIIARFSDTHHFNRKIIIILALLMGALGFSIYFFVDTI
                     WLFILLYAIFQGLFAPAMPQLYASARESINVSSSKDRAQFANTVLRSMFSLGFLFGPF
                     IGAQLIGLKGYAGLFGGTISIILFTLVLQVFFYKDLNIKHPISTQQHVEKIAPNMFKD
                     KTLLLPFIAFILLHIGQWMYTMNMPLFVTDYLKENEQHVGYLASLCAGLEVPFMIILG
                     VLSSRLHTRTLLIYGAIFGGLFYFSIGVFKNFYMMLAGQVFLAIFLAVLLGIGISYFQ
                     DILPDFPGYASTLFSNAMVIGQLGGNLLGGAMSHWVGLENVFFVSAASIMLGMILIFF
                     TKNQKITKEDMIST"
     gene            4606..5094
                     /locus_tag="NBI57_001408"
     CDS             4606..5094
                     /locus_tag="NBI57_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF456 domain-containing protein"
                     /protein_id="HCD1822361.1"
                     /translation="MTIILWLLIIAAFMLAFVGLIKPIIPSVLVLWVGFLIYQFGFHN
                     QHLSWVFYVSMALLTILILCADFLANKYFVNRFGGSKFGEYAALIGVVIGCFVLPPFG
                     IIIIPFILVFIVELIQGYSFERAVKVSIGSIVAFLTSSIAQAIIMFIMIVWFFIDALL
                     IN"
     gene            complement(5193..5882)
                     /locus_tag="NBI57_001409"
     CDS             complement(5193..5882)
                     /locus_tag="NBI57_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 family protein"
                     /protein_id="HCD1822362.1"
                     /translation="MKSTAQLTKENNVKSLRLSNTDREIFENYMTYMRSDFRVNPHDT
                     ELIINRILKQLLSAEQHGLLALDFFNHDPKAHAIKELKAMPNETFKNIFKYIYQHIVL
                     LIGIVSFLKGFLGFFMEKNGSNLYFVSFPFSVVVGFFIVFLFIWFSFKTIQLQCFNNS
                     NWIWIFTYLAIILLIVGFFYVFFIPQSLLAFGPYIQVSNWVFIIFSFIVMPIGLRIER
                     NISKKHSHTFL"
     gene            6009..6452
                     /locus_tag="NBI57_001410"
     CDS             6009..6452
                     /locus_tag="NBI57_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCD1822363.1"
                     /translation="MRTLNKDEHNYIKQIANIHETLLSQAESNYKCTKLSIALRYEMI
                     CSRLEHTNDKIYIYENEGQLIAFIWGHFSNEKSMVNIELLYVEPQFRKLGIATQLKIA
                     LEKWAKTMNAKRISSTIHKNNLPMISLNKDLGYQVSHVKMYKDID"
     gene            6519..6914
                     /locus_tag="NBI57_001411"
     CDS             6519..6914
                     /locus_tag="NBI57_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1822364.1"
                     /translation="MKKLIISIMAVMLFLTGCGKSQEKATLEKDIDNLQKENKELKDK
                     KEKLQQEKEKLADKQKDLEKEVKDLKPSKEDNKDDKKDEDKNKDKDKDKEASQDKQSK
                     DQTKSSDKDNHKKPTSTDKDQKANDKHQS"
     gene            7052..7351
                     /locus_tag="NBI57_001412"
     CDS             7052..7351
                     /locus_tag="NBI57_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1822365.1"
                     /translation="MHEQDFRILEGQDITLPELGRELENITGHTIADSTGEIKRVIAH
                     LPNFESDTDTFVATYRLNHQQDFIDATFTALKSDRARLKEVPVHVELISYISKSK"
     gene            7431..7973
                     /locus_tag="NBI57_001413"
     CDS             7431..7973
                     /locus_tag="NBI57_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCD1822366.1"
                     /translation="MIYCETERLILRDWHEDDLLPFQKMNANYDVRKYFPSLLSYRRS
                     ELDMRTMDAVIKDYGIGLFAVEDKESHQWIGFIGLNYIPETSDYPFKELPLYEIGWRL
                     LPEFWGKGLATEGAKATLKLAEEHQIYDVYSFTAEANKASQRVMEKIGMTVYDHFELP
                     NLSKYHLLKRQVRYYINLRK"
     gene            complement(8069..8635)
                     /locus_tag="NBI57_001414"
     CDS             complement(8069..8635)
                     /locus_tag="NBI57_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HCD1822367.1"
                     /translation="MKRIAVYCGASKGHDPSYVQKAYDLGKYFAEQGYELVFGAGSIG
                     IMGAIQDGVLNHGGKAIGVMPKMLDEHEITSQRLTELILVDSMHERKNKMAELADAFV
                     MAPGGAGSLEEFFEMYSWAQIGIHEKPIAIYNINGFFNPLQTMIDHMIEEGFIDPKYR
                     ALAPLCDTKESLIESILNFKPLGTRSYD"
     gene            complement(8637..9095)
                     /locus_tag="NBI57_001415"
     CDS             complement(8637..9095)
                     /locus_tag="NBI57_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HCD1822368.1"
                     /translation="MTHIIIDGDACPVVDSIIDLTTETGIFVTIIRSFSHFSNQLYPP
                     HVSTLYVDDGPDAVDYKIVQLSTKDDIVVTQDYGLASLLVDKVLIVMHHNGKIYNSKN
                     IQQLLDKRYINAQIRKQGGRHKGPPPFTKQDQKVFEQSLLKVIHRIKELD"
     gene            complement(9098..9781)
                     /locus_tag="NBI57_001416"
     CDS             complement(9098..9781)
                     /locus_tag="NBI57_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1822369.1"
                     /translation="MDKKKVIKFMINVLPIVLVPLIVERKRIKQHPDVQKVTDATSKV
                     ASKTSAAISNTASDVKEYVGDKKQDFENKRELKKFAREHDPAYIEKKGEKLAKQNRKD
                     ADKMNKILQKNIEKRHKEEQKAREKNEIQRIKDMKKSQKYEEKAGLTPNKLDEKTEKK
                     GDKLAEKNRKEIAKMNKKLQKNIEKRHKEEQKRQQEADKARIKSFKKYKDYVAKSASQ
                     QNKENNTEA"
     gene            complement(9953..10828)
                     /locus_tag="NBI57_001417"
     CDS             complement(9953..10828)
                     /locus_tag="NBI57_001417"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HCD1822370.1"
                     /translation="MFIIELIKGIILGVVEGLTEFAPVSSTGHMILVDDMWLKSSEFL
                     GSQSAFTFKIVIQLGSVFAAAWVFRERFLEILHIGKHKHVEGENDQQRRSKPRRLNLL
                     HVLVGMVPAGILGLLFDDFIEEHLFSVPTVMIGLFVGAIYMIIADKYSVKVKNPQTVD
                     QINYFQAFVIGISQAVAMWPGFSRSGSTISTGVLMKLNHKAASDFTFIMAVPIMLAAS
                     GLSLLKHYQDIQIADIPFYILGFLAAFTVGLIAIKTFLHLINKIKLIPFAIYRIVLVI
                     FIAILYFGFGIGKGI"
BASE COUNT         3804 a         1538 c         1878 g         3671 t
ORIGIN      
        1 aatgttaatt gtgaaaatgt atataaacat aaaaaaatca tgtataatat atgttgttaa
       61 ttaaacagtt cgaaagcgag atgacattat gggacgtaaa tggaataaca ttaaagaaaa
      121 aaaggcccaa aaagataaaa acacaagtag aatatatgcg aaatttggta aggagattta
      181 tgttgcagca aaatctggtg aacccaatcc agaatctaac caagctttaa ggttggtgct
      241 tgaacgcgct aagacatatt cagtgccgaa tcatattatt gaaaaagcaa tagataaagc
      301 taagggtgct ggagacgaaa actttgatca cctaagatat gaaggatttg gcccaagcgg
      361 atcaatgcta attgttgatg cgttaacaaa taatgtaaat cgtactgcct ctgatgtgcg
      421 agctgctttt ggtaaaaacg gcggtaatat gggtgtatct ggatcagttg cttatatgtt
      481 tgatcatgtg gcaacatttg gtattgaagg aaagtctgtt gacgaaatac ttgaaacatt
      541 aatggaacaa gatgtagatg taaatgatgt gattgacgat aatggattga caatagtcta
      601 tgctgaacca gatcaatttg cagtcgttca agatgcgctt cgtgcagcag gtgttgaaga
      661 atttaaagtt gctgaatttg aaatgttacc tcaaacagat attgaacttt ctgaagcgga
      721 ccaagtaaca tttgaaaaat taatcgatgc attagaagat ttagaagatg tacaaaacgt
      781 attccataat gtggatttga aataatgaaa tcagcagaac aatggattga tgaattgcaa
      841 cttgaatcac atcctgaagg tggtttctat agagagacaa ttcgagaagt attgaaagat
      901 ggacgcagag cgccgtttag tagtatttat tttttactta cagatgacaa tatttcgcat
      961 tttcatcgaa ttgatgctga tgaagtatgg tactatcatg ctggtgattc tctaacaatt
     1021 catatgataa atccggatgg ggaatatacg actgcaacat tgggtactga tatccaaaat
     1081 ggagatgtat tgcaatatgt agtgcctaaa ggaacaattt ttgcttcttc aatcgaaatt
     1141 tcaaatactt atagtttagt aggttgtatg tgtcaaccgg catttgagtt taagcagttt
     1201 gaattgttta agcaatctga attaattaca caatatccgc atcttaaatc agtgattgaa
     1261 aaatatgctt taaaataaaa atgatcaatg aagtggtttg aaggttgtta ataaaccttt
     1321 gagtcacttc atttttatat gtattcttga ttgaatcaga atagatttga tgcttcagct
     1381 gtttttaatg aaaatagcat taaatgattt tgaaaacgat aagagtgtgt tatttatatt
     1441 tttgaaaaat cacttttatg aagaaatgtg tgtactatct aattaatatt gtctatttta
     1501 agtaaattga agcgagttgt agtaaaataa taataaatat tttcatatga ttaacaaaaa
     1561 ctataaaact gtatcatgac atgacatcat ttatgtagtt ttatataata atgacaagga
     1621 gttggtgact gtgaaagtaa agtatataga taaacgtcac tggcgtcgcc taattgatag
     1681 ggaatacaca gaggtaaaag ttaataataa taggtttaag ggtattatag gcttagtcac
     1741 gatgaaaaag gttcgtgatc ctttagaggt gacggtagtt ggacaaaata tcattgtcgc
     1801 agatgacaat tataaatggt tgcaaatact acctgaaaag aaacgttata gtataactgt
     1861 aatgtttgat aataaaggca atccattaga atattatttt gatataaata tcaaaaatat
     1921 aacgcaaaaa ggtaatgcgc gtacagtaga tttatgttta gatgttttag cattaccaag
     1981 tggtgaatat gagttggtag atgaagatga tttaatgttt gcattagaaa gtgagcaaat
     2041 tacaaaaaag caatttcatg aagcatatat gattgcacat caaattatgg cagagttaga
     2101 aaatgatttt aaaggattcc aaaagaaaat catgtactgc tttaataaaa ttaatgcaaa
     2161 ggctcaaaaa aatcatcaaa agccacaaaa taaaactaat attgaaaaaa gcaaacaaat
     2221 aaaacctaag caatataatc aaactaaaaa tcaccaacaa caaaagaaaa actaagtaat
     2281 tcaagctgca gccatgccaa taaaattggt gaaattattc gttagtaatg aactcatgaa
     2341 tgcttgtagc catgaaagtt caataattga ataatttatg gggggaaatt atgaagattg
     2401 aagactatcg tttactaata acattagacg aaacgaaaac gttacgtaaa gcggctgaaa
     2461 ttttatatat atctcaacct gctgttacac aaagactaaa agctattgaa aatgcttttg
     2521 gagtagatat ttttatcaga acaaaaaaac aattgattac aacaactgaa ggaacaatga
     2581 ttattgagca tgcccgtgac atgttgaaaa gagagcgatt attttttgac aaaatgcagg
     2641 cacatattgg tgaagtgaat ggaacaatat caatcgggtg ttcttctttg attggacaaa
     2701 ccttacttcc tgaagttttg agcctatata atgcccaatt tcctaatgtt gaaatacaag
     2761 tgcaagttgg ttcaactgaa caaattaaag caaatcatag agattatcat gttatgataa
     2821 ctcgtggaaa taaggtaatg aatttagcta acacacattt atttaatgat gatcattatt
     2881 ttatttttcc aaaaaataga cgagatgatg ttacaaagtt accatttata gagtttcaag
     2941 ctgatccgat ttatataaat caaataaaac aatggtataa cgataattta gaacaagatt
     3001 accatgcaac tattacagtg gatcaagtag caacttgcaa agaaatgttg attagtggtg
     3061 taggtgttac aattttgccg gaaattatga tgaaaaatat cagcaaagaa caatttgagt
     3121 ttgaaaaagt agaaattgat aatgaaccgc tgattcgttc gacatttatg agttatgatc
     3181 cgagcatgtt gcaattgcca caagttgatt cttttgtaaa tctcatggcg agctttgttg
     3241 aacaaccaaa ggcgtagttt tagactaatt taaggtttgt atttaatttt aaactattcg
     3301 gttaaattga acgtagttgt ttgctaacgc accaacagca aaagagcccc taattaataa
     3361 attaaaaggg gctctaagaa gcggggttat ggatatgttt gcagcgttat tacaaataaa
     3421 gaattataaa ctctttgttg ctaatatgtt tctactaggt atgggtattg cggttacggt
     3481 cccatatctt gttctttttg caactaaaga tttaggtatg acaacaaatc agtatggatt
     3541 acttctagca tctgcagcga ttagccagtt tacagtaaat tcaattattg ctagattttc
     3601 ggatacgcat cactttaata gaaaaattat tattattctc gcattattaa tgggtgcgct
     3661 tggtttttca atatactttt ttgtagatac aatctggtta ttcatattac tatatgcgat
     3721 tttccaagga ttatttgcac cagcaatgcc ccaactttac gcatctgcta gagaatctat
     3781 caatgtttca agctctaaag atagagctca atttgccaac acagtattac gttcaatgtt
     3841 ctcattgggc tttttatttg gtccatttat tggtgcccaa ttaatcggtt taaaaggcta
     3901 tgctggattg tttggtggaa caataagtat cattttattt actttagtac ttcaagtgtt
     3961 tttctataag gatttaaaca ttaaacaccc tattagtacg caacaacatg ttgaaaaaat
     4021 tgctcctaat atgtttaaag acaaaacgct tttattacca tttattgcat ttattttatt
     4081 acacattgga caatggatgt atacgatgaa tatgccttta tttgttactg attatttaaa
     4141 agaaaatgag caacatgtcg gttatttagc tagtttatgt gctggtttag aagtgccatt
     4201 tatgatcatt cttggcgttt tatcatctag attacatact cgaacattgt tgatttatgg
     4261 agcgattttt ggtggtttat tctacttcag cattggggta tttaaaaact tctatatgat
     4321 gttagcagga caggtatttt tagctatttt cttagcggtt cttttaggaa ttggtattag
     4381 ttatttccaa gatatcttac cagattttcc aggatacgcc tcaacactat tttctaatgc
     4441 aatggttatt ggacagttag gcggtaacct attaggtggt gctatgagtc actgggtagg
     4501 tttggaaaat gtattttttg tatcagcagc atcaatcatg ttaggtatga tacttatatt
     4561 ctttactaaa aatcaaaaaa ttacaaaaga ggatatgata tcaacatgac aattatttta
     4621 tggctactta tcatcgctgc cttcatgtta gcatttgttg ggttgattaa gccgattatt
     4681 ccttctgttt tagtattatg ggttggcttt ctaatctatc aatttggctt tcataatcag
     4741 catttatcat gggtgtttta tgtatctatg gcattgctaa caatactaat tttatgtgcg
     4801 gactttttag ctaataaata ttttgtgaat cgcttcggtg gttctaagtt tggagagtat
     4861 gcagctttaa ttggtgtggt tattggatgt tttgttttac cgccatttgg aattattatt
     4921 atacctttta ttttggtatt catagttgaa ttaatacaag gctattcatt tgaaagagca
     4981 gttaaagtaa gtataggttc aatcgtagca tttttaacaa gtagtatagc tcaagcaatc
     5041 attatgttta taatgattgt atggttcttt atagatgctt tattgattaa ttaataaaaa
     5101 gcttattgca aaatatgttt ttcggtaact gtaatttagt gattttatca ttaacagtac
     5161 caaattcgca tatgatgtaa taagcttttt gtttataaaa atgtatgaga atgttttttc
     5221 gaaatatttc tttcaatgcg taatccaata ggcataacta taaatgaaaa tataataaat
     5281 acccaattgc ttacctgaat atatggtcca aaggcaagta atgattgtgg aataaagaat
     5341 acatagaaaa atcctacaat tagtagaatg attgctaaat aggtgaatat ccatatccaa
     5401 tttgaattgt taaaacactg cagttgtatt gttttaaaac taaaccatat aaataaaaag
     5461 acaataaaga atccaacgac tactgaaaac gggaatgaaa caaaatataa attacttcca
     5521 tttttttcca tgaaaaatcc taaaaatcct ttgagaaaac taacaatccc aattaataga
     5581 acgatgtgtt gatagatata tttaaaaata tttttaaatg tttcattagg catcgctttt
     5641 agttctttta ttgcatgtgc ttttgggtcg tgattgaaaa aatctaaggc taataaacca
     5701 tgttgttctg cgcttaataa ttgtttgagt atacggttaa taattaactc tgtatcatga
     5761 gggttgacgc gaaagtcaga gcgcatataa gtcatataat tctcgaagat ttctctatca
     5821 gtattgctta atcttaatga tttaacatta ttttcttttg ttaattgcgc agtacttttc
     5881 attgttactt aagcgctcct ttaaaaatgt ttaattccta attaaaatgg aaatgatttt
     5941 atagtattaa taaagtcaat catatcatat taaacgcata aatataacga ttaatattgg
     6001 agaggaaaat gaggacactt aataaagatg aacataatta tatcaagcaa atagctaata
     6061 tacacgagac attattgtcg caagcagaat ccaactataa atgtactaaa ctgagtattg
     6121 ctcttaggta cgagatgata tgttcaagat tagaacatac aaatgataaa atttatatat
     6181 atgaaaatga aggtcaatta atagcgttta tttggggaca ttttagtaat gaaaaaagta
     6241 tggttaacat tgaactgcta tatgttgaac cacaatttcg caaactggga atagctacgc
     6301 aactgaagat tgcgcttgaa aaatgggcaa aaactatgaa tgcaaagcga ataagcagta
     6361 caattcataa aaataatttg ccaatgatat ctttgaataa agatttaggt tatcaagtga
     6421 gtcatgtgaa aatgtataaa gatattgatt agaattagga ttatgttgct aattcatgtt
     6481 aaaattaaaa aagatttaat gacgttaagg agttttatat gaagaaatta atcatcagta
     6541 ttatggcggt catgctattt ttaacaggtt gtggtaaaag tcaagagaaa gccactctgg
     6601 aaaaggatat cgataattta caaaaagaaa ataaagaatt aaaagacaaa aaagaaaagc
     6661 ttcaacaaga aaaagaaaaa ttagcagata agcaaaaaga ccttgaaaaa gaagtgaaag
     6721 atttaaaacc ttcaaaagaa gataacaagg atgataaaaa agacgaagac aaaaataaag
     6781 acaaagataa agataaagag gcatcacaag ataagcaatc aaaagatcaa actaagtcat
     6841 cggataaaga taatcacaaa aagcctacat caacagacaa agatcaaaaa gctaatgaca
     6901 aacaccaatc ataatcgtat tgcttacttg ttatagatga aaggtacagc gttttaaacc
     6961 ttattttaag ggtatgtatt aattaaaatg tggtcatgat tgaaacagaa cgtaaaaata
     7021 gacaacgtaa ttaataaagg agagaaacgg catgcatgaa caagatttta gaattttaga
     7081 gggtcaagat attactttgc cagaattagg tagagaatta gaaaatatta caggacatac
     7141 gattgctgat tctactggcg aaattaagcg tgtaattgca catttaccaa actttgagtc
     7201 cgatacagat acttttgttg ctacatatcg tttaaaccat caacaagatt ttatagatgc
     7261 aacttttact gcgctgaaat cagatagagc acgtttaaaa gaagtgccag ttcatgttga
     7321 acttataagt tatatttcta aatcaaaata aactgctatc taaaacgcaa agttgatcaa
     7381 aatatcgatt ttgtgttttt tattgagaaa ttatatagga gtgtcaatcg atgatttatt
     7441 gtgaaacaga gcgtttaata ttaagagact ggcatgaaga tgatctgtta ccttttcaaa
     7501 aaatgaatgc gaattatgac gtacgtaaat attttccaag tttattgagt tatcgtcgtt
     7561 cagaattaga tatgagaact atggatgcgg ttattaaaga ttatggcatt ggattatttg
     7621 ctgtagaaga taaagagtcc catcaatgga taggctttat aggtttgaat tatattccag
     7681 aaacaagcga ttatccattt aaagaattac cgctttatga aataggttgg cgcttgttgc
     7741 ctgaattttg gggaaaagga ttagcaactg aaggcgcaaa ggcaacgttg aagttagcag
     7801 aagaacatca aatatacgat gtctatagtt ttacagcaga agcaaataaa gcctcacaac
     7861 gtgtaatgga aaaaattggc atgacagtgt atgatcattt cgaattaccc aatctaagta
     7921 agtatcattt attaaaaagg caagtgcgct attacattaa tcttcgaaag tgaaaaattt
     7981 atacataatc gtaacaaaca cccctaacat tgtttagctg atgataacaa tattaggggt
     8041 gtttgtttat tttttaacct tagaatgatt aatcgtatga acgagtaccc agaggtttga
     8101 aatttaatat tgattctatt aatgattcct tagtgtcgca taacggtgca agagcacgat
     8161 acttaggatc aataaaacct tcctcaatca tatggtcaat cattgtttgt agtggattga
     8221 aaaagccatt aatattataa atggcaatag gcttttcatg gatacctatt tgagcccaac
     8281 tatacatttc gaaaaattct tctagtgaac ctgcgccacc aggagccatg acaaatgcat
     8341 ctgcaagttc tgccatttta tttttacgtt catgcataga atcaactaaa attaattcag
     8401 ttaaacgttg gcttgtgatt tcatgttcat ctaacatttt aggcatgacg ccaatagctt
     8461 tgccgccatg atttaataca ccatcttgaa tggcacccat aatgccaatt gaccctgcac
     8521 caaatactaa ttcataacct tgttcagcaa aatatttacc taaatcgtat gctttttgta
     8581 catatgaagg gtcatgacct ttgcttgcac cacaataaac tgcgattcgt ttcatgttaa
     8641 tccagctcct taattcgatg aatgactttt aatagtgatt gttcaaacac tttttgatct
     8701 tgctttgtaa aaggtggggg acctttgtgg cgaccacctt gttttctaat ttgtgcattt
     8761 atatatcgtt tatctaatag ttgttgaata tttttggaat tgtatatctt cccattatga
     8821 tgcatgacaa ttaagacttt gtcgactaat aaacttgcga gtccataatc ttgagtgact
     8881 acgatatcat ccttcgttga taattgaaca attttgtaat caactgcatc tggtccatca
     8941 tcaacatata atgttgatac atgtggagga tataattggt tcgaaaaatg gctgaagctc
     9001 cgaataattg tcacaaaaat gcctgtctca gttgttaaat ctataataga atcaacaaca
     9061 ggacaagcat ctccatcaat aataatatgt gtcacaatta tgcctctgta ttgttttctt
     9121 tattttgttg agaggcgctt ttggcaacat aatctttata ttttttaaat gacttgatgc
     9181 gtgctttatc agcttcttgt tggcgttttt gttcttcttt gtgtcgtttt tcaatatttt
     9241 tttgtaactt tttattcatt ttagcgattt ctttgcgatt tttttcagct agtttatcgc
     9301 cttttttctc agttttctca tctaatttat taggtgttaa gcctgctttt tcttcgtatt
     9361 tttgtgattt tttcatatct ttaatacgtt gtatttcatt cttttcgcgg gctttttgct
     9421 cttctttatg acgcttttcg atattttttt gaagtatttt attcatttta tcagcgtctt
     9481 tacgattttg tttagctaat ttttcgcctt ttttctcaat ataggcagga tcatgttctc
     9541 tagcaaactt tttaagttca cgtttatttt caaaatcttg ttttttatcg ccgacatatt
     9601 ctttaacatc actcgctgtg ttactgattg ctgcagatgt ttttgaagca actttacttg
     9661 tagcatctgt aactttttgt acgtccggat gttgtttgat acgtttacgt tcaacaatta
     9721 acggtaccaa tacaattggt aatacattaa tcataaattt gatgactttt ttcttatcca
     9781 tagatcttgc ctccataatt actttattaa ttttacatac cctatgatac atcaatataa
     9841 acgatgatag tagtgaatca ctattaagta tttcagatgt tttttaaaag aagacaataa
     9901 aaactgccaa tcaagtggtt ccttaattga cagtctatat tttaaaagga aattaaatac
     9961 ctttaccaat gccaaatccg aagtaaagta tagcaataaa gattactaat acaattctgt
    10021 aaatggcaaa tggaattagt ttgattttgt taattagatg caagaatgtt ttgattgcaa
    10081 ttagtccaac agtaaatgca gctaaaaagc ctaaaatata aaaaggtata tcagcaatct
    10141 gaatatcttg ataatgtttt aataaagata aaccactagc tgctaacata attggaacag
    10201 ccataataaa tgtgaagtcc gatgctgctt tatgatttaa tttcattaat accccagttg
    10261 aaattgttga gcctgaacgg ctgaaaccag gccacatagc tactgcttga gagataccaa
    10321 ttacaaacgc ttggaaataa ttgatttgat ccactgtttg tgggttttta actttaactg
    10381 aatacttatc agcaataatc atatagatag cacctacgaa taagccaatc ataacagttg
    10441 gcacactaaa taaatgttct tcgatgaaat catcaaatag taagcctaaa atacctgctg
    10501 gcaccatacc cactaataca tgtaataaat ttaaacgtct tggctttgaa cgtctttgtt
    10561 gatcgttttc tccttcaaca tgtttgtgtt taccaatatg taaaatctct aagaagcgtt
    10621 cgcggaatac ccatgctgct gcaaagacgg atcctaattg gatgacgatt ttaaatgtaa
    10681 atgctgattg agaacctaaa aattcagatg attttaacca catatcatca actagaatca
    10741 tatgtccagt agaggaaaca ggtgcaaatt ctgttaatcc ttcgacgacc cctaagataa
    10801 taccttttat taattcaatg ataaacataa tgtacccact ttcattactc aatttaattt
    10861 atttaaatat caaaattacc atatcatgat a
//
LOCUS       DAHHSC010000072        10826 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.072, whole genome shotgun sequence.
ACCESSION   DAHHSC010000072 DAHHSC010000000
VERSION     DAHHSC010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 10826)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10826)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10826
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.072"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            262..2451
                     /locus_tag="NBI57_001418"
     CDS             262..2451
                     /locus_tag="NBI57_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HCD1822371.1"
                     /translation="MNNEYPYSADEVLHKAKSYLSADEYEYVLKSYHIAYEAHKGQFR
                     KNGLPYIMHPIQVAGILTEMRLDGPTIVAGFLHDVIEDTPYTFEDVKEMFNEEVARIV
                     DGVTKLKKVKYRSKEEQQAENHRKLFIAIAKDVRVILVKLADRLHNMRTLKAMPREKQ
                     IRISRETLEIYAPLAHRLGINTIKWELEDTALRYIDNVQYFRIVNLMKKKRSEREAYI
                     ETAIDRIRTEMDRMNIEGDINGRPKHIYSIYRKMMKQKKQFDQIFDLLAIRVIVNSIN
                     DCYAILGLVHTLWKPMPGRFKDYIAMPKQNLYQSLHTTVVGPNGDPLEIQIRTFDMHE
                     IAEHGVAAHWAYKEGKKVSEKDQTYQNKLNWLKELAEADHTSSDAQEFMETLKYDLQS
                     DKVYAFTPASDVIELPYGAVPIDFAYAIHSEVGNKMIGAKVNGKIVPIDYILQTGDIV
                     EIRTSKHSYGPSRDWLKIVKSSSAKGKIKSFFKKQDRSSNIEKGRMMVEVEIKEQGFR
                     VEDILTEKNIQVVNEKYNFANEDDLFAAVGFGGVTSLQIVNKLTERQRILDKQRALNE
                     AQEVTKSLPIKDNIITDSGVYVEGLENVLIKLSKCCNPIPGDDIVGYITKGHGIKVHR
                     TDCPNIKNETERLINVEWVKSKDATQKYQVDLEVTAYDRNGLLNEVLQAVSSTAGNLI
                     KVSGRSDIDKNAIINISVMVKNVNDVYRVVEKIKQLGDVYTVTRVWN"
     gene            2463..2915
                     /locus_tag="NBI57_001419"
     CDS             2463..2915
                     /locus_tag="NBI57_001419"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HCD1822372.1"
                     /translation="MKVVVQRVKEASVTNDTLNNQIKKGYCLLVGIGQNSTEQDADVI
                     AKKIANARLFEDDNNKLNFNIQQMNGEILSVSQFTLYADVKKGNRPGFSNSKNPDQAV
                     KIYEYFNDALRAYGLTVKTGEFGTHMNVSINNDGPVTIIYESQDGKIQ"
     gene            2912..3787
                     /locus_tag="NBI57_001420"
     CDS             2912..3787
                     /locus_tag="NBI57_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HCD1822373.1"
                     /translation="MKKIEAWLSKKGLKNKRTLIVVIAFVLFIIFLFLLLNSNSEDSG
                     NITITENAELRTGPNAAYPVIYKVEKGDHFKKIGKVGKWIEVEDTSSNEKGWIAGWHT
                     NLDIVADNTKEKNPLQGKTIVLDPGHGGSDQGASSNTKYKSLEKDYTLKTAKELQRTL
                     EKEGATVKMTRTDDTYVSLENRDIKGDAYLSIHNDALESSNANGMTVYWYHDNQRALA
                     DTLDATIQKKGLLSNRGSRQENYQVLRQTKVPAVLLELGYISNPTDETMIKDQLHRQI
                     LEQAIVDGLKIYFSA"
     gene            4248..5510
                     /locus_tag="NBI57_001421"
     CDS             4248..5510
                     /locus_tag="NBI57_001421"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HCD1822374.1"
                     /translation="MIKIPRGTQDILPEDSKKWRYIENQLDELMTFYNYKEIRTPIFE
                     STDLFARGVGDSTDVVQKEMYTFKDKGDRSITLRPEGTAAVVRSYIEHKMQGNPNQPI
                     KLYYNGPMFRYERKQKGRYRQFNQFGVEAIGAENPSVDAEVLAMVMHIYQSFGLKHLK
                     LVINSVGDMASRKEYNEALVKHFEPVIHEFCSDCQSRLHTNPMRILDCKVDRDKEAIK
                     TAPRITDFLNEESKAYYEQVKAYLDDLGIPYIEDPNLVRGLDYYTHTAFELMMDNPNY
                     DGAITTLCGGGRYNGLLELLDGPSETGIGFALSIERLLLALEEEGIELDIEENLDLFI
                     VTMGDQADRYAVKLLNHLRHNGIKADKDYLQRKIKGQMKQADRLGAKFTIVIGDQELE
                     NNKIDVKNMTTGESETIELDALVEYFKK"
     gene            5526..7292
                     /gene="aspS"
                     /locus_tag="NBI57_001422"
     CDS             5526..7292
                     /gene="aspS"
                     /locus_tag="NBI57_001422"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HCD1822375.1"
                     /translation="MSKRTTYCGLVTEAFLGQEITLKGWVNNRRDLGGLIFVDLRDRE
                     GIVQVVFNPAFSEEALKIAETVRSEYVVEVQGTVTKRDPETVNPKIKTGQVEVQVTNI
                     KVINKSETPPFSINEENVNVDENIRLKYRYLDLRRQELAQTFKMRHQITRSIRQYLDD
                     EGFFDIETPVLTKSTPEGARDYLVPSRVHDGEFYALPQSPQLFKQLLMISGFDKYYQI
                     VKCFRDEDLRADRQPEFTQVDIEMSFVDQEDVMQMGEEMLKKVVKEVKGVEINGAFPR
                     MTYKEAMRRYGSDKPDTRFEMELIDVSQLGRDMDFKVFKDTVENDGEIKAIVAKGAAE
                     QYTRKDMDALTEFVNIYGAKGLAWVKVVEDGLTGPIGRFFETENVETLLTLTGAEAGD
                     LVMFVADKPNVVAQSLGALRVKLAKELGLIDETKLNFLWVTDWPLLEYDEDAKRYVAA
                     HHPFTSPKEADIAKLGTAPEEAEANAYDIVLNGYELGGGSIRIHDGELQEKMFEVLGF
                     TKEQAQEQFGFLLDAFKYGAPPHGGIALGLDRLVMLLTNRTNLRDTIAFPKTASATCL
                     LTNAPGEVSDKQLEELSLRIRH"
     gene            7370..7560
                     /gene="ssrS"
                     /locus_tag="NBI57_001423"
     ncRNA           7370..7560
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="NBI57_001423"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            7625..7753
                     /locus_tag="NBI57_001424"
     CDS             7625..7753
                     /locus_tag="NBI57_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1822376.1"
                     /translation="MGFIAFNKCFLVEFWENNQSDNAKYKILSYYVLMYKSVKEVK"
     gene            7753..8526
                     /locus_tag="NBI57_001425"
     CDS             7753..8526
                     /locus_tag="NBI57_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA threonylcarbamoyladenosine dehydratase"
                     /protein_id="HCD1822377.1"
                     /translation="MKHQFSRNELAIGQQGLDLLKQQTVVILGVGGVGSFAAEALART
                     NIGHIILIDKDDVDITNVNRQLHALTTTVGQSKVSLMEERIKLINPDCKVTPLHMFYT
                     EETYEEIFDNYDIDYFVDASDTIMYKVHLMKECLDRGIKVISSMGAANKTDPTRFTIS
                     DISKTHTDPMAKIIRQKLKKLGIRKGIPVVFSDESPIVIREDVKDIVGDKNAINRKGQ
                     MPPSSNAFVPSVVGLICASYVVNDVLKDIPVRRIKDKGQ"
     gene            complement(8687..9961)
                     /locus_tag="NBI57_001426"
     CDS             complement(8687..9961)
                     /locus_tag="NBI57_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HCD1822378.1"
                     /translation="MSTEPLASRMRPKNIDEIISQQHLVGPKGIIRRMVDTKKLTSMI
                     FYGPPGIGKTSIAKAISGSTQYKFRQLNAVTNTKKDMQLVVEEAKMSGQVILLLDEIH
                     RLDKAKQDFLLPHLENGKIVLIGATTSNPYHAINPAIRSRAQIFELYPLNDEDVRQAL
                     TRAIEDDENGLKTYQPKIDEDAMTYFSTQSQGDVRSALNALELAVLSADNDKDGYRHV
                     TLQDAKDCLQKGAFVSDKDGDMHYDVMSAFQKSIRGSDVNAALHYLARLIEAGDLPTI
                     VRRLLVISYEDIGLASPNAGQRTLAAIESAERLGLPEARIPLSQAVIELCLSPKSNSA
                     MSAIDSALSDIRNGHVGQIPNHLKDGHYQGAKDLGRSIGYKYPHQYVNGYVSQQYLPD
                     KLKNKIYYEPKTTSKSEQQLKEIYNNLLKQRP"
     gene            10046..10468
                     /locus_tag="NBI57_001427"
     CDS             10046..10468
                     /locus_tag="NBI57_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002458938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HCD1822379.1"
                     /translation="MKISTKGRYGLTLMISLAKKEGQGCISLKSIAEENNLSDLYLEQ
                     LVGPLRNAGLIRSVRGAKGGYQLRVPAEEISAGDIIRLLEGPITFVESIESEPPAQKQ
                     LWIRMRDAVRDVLDNTTLKYLAEYVDTSEDLDGYMFYI"
     gene            10568..10750
                     /locus_tag="NBI57_001428"
     CDS             10568..10750
                     /locus_tag="NBI57_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HCD1822380.1"
                     /translation="MADESKFDQFKGNVKETVGNVTDNKELEKEGQQDKATGKAKEVV
                     ENAKNKITDAIDKLKK"
BASE COUNT         3878 a         1512 c         2124 g         3312 t
ORIGIN      
        1 ttcataaatt gtagtatcat agaactaata tttaaaaaat gaaacagtag atttaggtcg
       61 aatttttgta aaagttttaa aagtaggaat agtatacaaa ttaaactcgc tcaagtaaaa
      121 ttaatattac gattaatgac gacaggataa atatttatcg tcgacggacg tatgattggt
      181 gtgggacaaa tactattcaa caagagtacc taaatcattg tttaaggcga agtaataaat
      241 atgaatgggg tgtatcatat aatgaacaac gaatatccat atagtgcaga tgaagttctt
      301 cacaaagcaa aatcatattt gtcagcagat gaatatgagt atgttttaaa aagctatcat
      361 attgcttatg aagcacataa aggtcagttc cgaaaaaacg gattaccata cattatgcat
      421 cctatacaag ttgcaggtat tttaacagaa atgcgattag acggaccgac gattgtcgca
      481 ggttttttgc atgatgtaat tgaagataca ccgtatacat ttgaagatgt aaaagaaatg
      541 ttcaatgaag aagttgctcg aattgttgat ggtgtgacga agcttaaaaa agtaaaatac
      601 cgctcaaaag aagaacaaca agctgaaaat catcgcaagt tatttattgc gattgccaaa
      661 gatgtacgcg taattttggt gaaattagca gacagattac ataatatgcg taccttgaaa
      721 gccatgccgc gtgaaaaaca aattagaatt tctcgagaaa cattagaaat ttatgcacct
      781 ttagcacatc gtcttggtat taatacaatt aaatgggaac tagaagatac ggctcttcgt
      841 tatattgata atgtgcaata ttttagaata gtcaatttaa tgaagaagaa acgtagtgaa
      901 cgtgaagcgt atatcgaaac ggctattgat agaatacgta ctgaaatgga ccgaatgaat
      961 atcgaaggcg atataaatgg tagacctaaa catatttaca gtatttatcg gaaaatgatg
     1021 aagcagaaaa aacaatttga tcaaattttt gatttgttgg cgatacgtgt tattgtcaat
     1081 tctattaatg attgttatgc gatacttggg ttggtgcata cgttatggaa accgatgcca
     1141 ggacgtttta aagattatat tgcaatgcct aaacaaaatt tgtatcagtc attgcatact
     1201 acagtagtag gtccaaatgg agacccgctc gaaatccaaa tacgaacgtt tgatatgcac
     1261 gaaattgctg agcatggtgt tgcagcacac tgggcttaca aagaaggtaa aaaagtaagt
     1321 gaaaaagatc aaacttatca aaataagtta aattggttaa aagaattagc tgaagcggat
     1381 catacatcgt ctgacgctca agaatttatg gaaaccttaa aatatgactt acagagtgac
     1441 aaagtatacg catttacccc agcgagtgat gttattgagt tgccatatgg tgctgtgccg
     1501 attgattttg cttatgcgat tcacagtgaa gtaggtaata agatgattgg tgccaaggtg
     1561 aatggcaaaa ttgtaccaat tgactatatt ttacaaacag gcgatattgt tgaaatacgt
     1621 actagtaaac attcatatgg accaagtcgt gattggttga aaattgttaa atcgtctagt
     1681 gccaaaggta aaattaaaag tttcttcaaa aaacaagatc gttcatctaa tattgaaaaa
     1741 ggccgaatga tggttgaagt tgaaataaaa gagcaaggat ttagagtcga agatattttg
     1801 acagagaaaa atattcaggt tgttaatgaa aaatataact ttgcaaatga agatgattta
     1861 ttcgcagctg taggatttgg cggcgtgaca tccttacaga ttgttaataa attaactgaa
     1921 agacaacgta ttttagataa acaacgtgct ttaaatgaag cacaagaagt tacgaaatca
     1981 ttgcctatta aagacaacat cattactgat agtggtgtct atgtagaagg tttagaaaat
     2041 gtacttatca agttgtcaaa atgttgtaat cctataccag gtgatgatat tgtaggttat
     2101 atcaccaaag gtcacggtat taaagtacat cgcactgatt gcccaaatat taagaacgaa
     2161 actgaacgac taattaatgt tgaatgggta aaatcaaaag acgcaactca aaaatatcag
     2221 gttgatttag aggtaactgc gtatgaccga aatggcttgt tgaatgaagt actacaagct
     2281 gttagctcga cagccggcaa tttaattaaa gtttcaggac gttcagatat tgataaaaat
     2341 gcaataataa atattagtgt catggtgaaa aacgtgaatg atgtttatcg tgtggtagaa
     2401 aagatcaaac aacttggtga tgtttataca gtaacaagag tttggaacta gaggtgcaaa
     2461 atatgaaagt agttgtacaa agagttaaag aagcatcggt gacgaatgat acattaaata
     2521 atcaaatcaa aaaaggatat tgtttattag tcggtatcgg tcagaactct acagagcaag
     2581 atgcagatgt aattgcaaag aaaattgcta atgcaagatt atttgaagat gacaataata
     2641 aattaaactt taatatccaa caaatgaatg gtgaaatact atcagtttca caatttactc
     2701 tctatgcaga tgtaaaaaaa ggtaaccgtc caggtttctc aaattctaaa aatcctgatc
     2761 aagcggtaaa aatttatgag tattttaatg atgcgctacg agcgtatggt cttactgtga
     2821 aaacaggtga atttggaaca cacatgaatg ttagcataaa taatgatggt ccagtcacta
     2881 ttatttatga aagtcaggac ggcaaaattc aatgaaaaaa atagaggcat ggttatctaa
     2941 aaagggtctt aaaaataaac gtactctaat agtagtgatt gcctttgtct tatttatcat
     3001 ctttttattt ttattgctga atagcaatag tgaagatagt gggaacatca cgataactga
     3061 aaatgctgaa ttacgtacag gtccaaacgc tgcgtatcca gtcatatata aagttgagaa
     3121 aggtgaccat tttaaaaaga ttggtaaagt aggtaaatgg attgaagttg aagatacatc
     3181 cagtaatgaa aaaggttgga tagctggatg gcacacaaat ttagatattg tcgcggataa
     3241 tacgaaggag aaaaatcctt tgcaaggtaa aacaatagtg cttgatcctg gtcatggagg
     3301 tagtgaccag ggtgcttcaa gcaatactaa atataaaagt ttagaaaaag actatacgtt
     3361 gaaaacagca aaagaattgc agcgtacttt agaaaaagaa ggcgcaactg ttaagatgac
     3421 aagaacagac gatacatatg tttcactaga aaatcgtgat atcaaaggcg atgcctattt
     3481 gagtatacat aatgatgcat tagaatcatc taatgcaaat ggaatgacag tttattggta
     3541 tcatgataat caaagagctt tagcagatac gttagacgct acgattcaga agaaaggttt
     3601 actttctaat cgcggttcaa gacaagaaaa ttatcaagtg ttaagacaaa caaaagttcc
     3661 tgctgtttta ttagaattag gttatattag taacccaact gatgaaacga tgattaaaga
     3721 tcaattacat agacaaattt tagaacaagc aattgttgat ggacttaaaa tttatttttc
     3781 tgcgtagggc ttgcaaaaat atgtgaaagt agttatcatt gatattgaat tttataacta
     3841 aaaccgttag tattcttgaa atggtaaatg aaataggtag caatctaact aagattgtgt
     3901 aggaatataa tccatagact gaaagattat gctgagtagt ttatatacat tgaacacaag
     3961 aagaggtgct ttatgaaaag taaagccgtt aaacgtacgt taaacgtttt gagtgggttt
     4021 atttaatgca cgcttataaa aagtaatgat gattacaatt aggcatgttt tttaaaccaa
     4081 tgagggtggc aacacggata ttcgtccctt gtgcagtttc aggctgtgca agggcttttt
     4141 cttttgcttt taaaattatg atttatcatg caaagttaag tgatcgtata tagttttagt
     4201 tttaaaaagg taattaaata aaatagtttg ccgagggaga tgtcaaaatg attaaaatac
     4261 ctagagggac gcaggatatt ttacctgaag attcaaagaa atggcgttac attgaaaatc
     4321 aattagatga attaatgaca ttttataatt ataaagaaat aagaacacca atttttgaaa
     4381 gtacagatct ttttgcaaga ggtgttggtg attcaaccga tgtcgtacaa aaagaaatgt
     4441 atacatttaa agataaaggc gatagaagta ttacattaag acctgaggga acagctgcag
     4501 ttgtgcgttc atatattgaa cataaaatgc aaggtaatcc aaaccaacca attaaacttt
     4561 attacaatgg accgatgttt agatatgaac gtaagcaaaa agggcgctat cgtcaattta
     4621 atcaatttgg tgtagaagct attggtgctg aaaatcctag cgtagatgca gaagtattag
     4681 ctatggttat gcatatttat caatcatttg gattaaaaca tttaaagctt gttattaata
     4741 gtgtagggga tatggcgtct cgaaaagaat ataacgaagc gttagtgaaa cactttgaac
     4801 cagtaattca tgaattttgt tcagattgtc aatcacgttt gcatacaaat ccgatgcgaa
     4861 ttttggattg taaagttgac cgtgataaag aagcgattaa gactgcacct agaatcactg
     4921 atttcttaaa tgaggaatct aaggcatatt atgaacaagt aaaagcttat ttagatgatt
     4981 taggtattcc atatattgaa gatcctaact tagttcgtgg attggattat tatacacata
     5041 cagcatttga attaatgatg gataatccta actatgatgg tgccattaca acgctttgtg
     5101 gtggtggccg ttataatggt ttattagaat tgctagatgg tccaagtgaa acaggtattg
     5161 gttttgcgct aagtatagaa cgattattgc ttgcacttga agaagaaggt atcgaattag
     5221 atattgaaga aaacttagat ttattcattg ttacaatggg tgatcaagca gatcgatatg
     5281 ctgtgaagtt attaaatcat ttgagacata atggtattaa agcagataaa gactacttac
     5341 agcgtaaaat taaaggacaa atgaaacaag cagaccgttt aggtgccaag tttacaatcg
     5401 ttattggtga tcaagaatta gaaaataata aaatcgatgt taaaaatatg acaactggtg
     5461 aatctgaaac aattgaatta gacgcattag tcgaatattt taagaagtag agagggcgtt
     5521 aaaatatgag taagagaaca acttattgtg gattagttac tgaagcattt ttaggacaag
     5581 aaattacatt aaaaggatgg gttaacaatc gtcgtgacct aggtggattg attttcgttg
     5641 atttaagaga tagagaagga attgtacaag tcgtgtttaa tcctgcattt tcagaagagg
     5701 cattgaaaat tgctgaaaca gtacgttctg aatatgttgt agaagttcaa ggtacagtta
     5761 cgaagcgtga ccctgaaaca gttaatccta aaattaaaac tggccaagtt gaagtacaag
     5821 ttacaaatat taaagtgatt aataaatctg agacaccacc attttctata aatgaagaaa
     5881 atgttaacgt tgatgaaaat attcgattaa aataccgtta tttagattta cgtcgtcaag
     5941 agttagcgca aacatttaaa atgagacatc aaattacacg ttctattcgt caatatttgg
     6001 atgatgaagg gttctttgac atcgaaacac cagtactaac gaagtcaaca cctgagggtg
     6061 cacgtgacta tttagtacca tctcgtgttc atgatggtga attttatgca ttaccacaat
     6121 caccacaatt atttaagcaa ttattgatga ttagtggatt tgacaaatac taccaaatcg
     6181 taaaatgctt ccgtgacgaa gatttacgtg cagatcgtca acctgaattc acacaagtcg
     6241 atattgaaat gagttttgta gatcaagaag atgtgatgca aatgggtgaa gaaatgctta
     6301 aaaaagttgt taaagaagtt aaaggcgttg aaattaatgg cgctttccca cgcatgacat
     6361 ataaagaagc gatgcgtcgc tatggttctg ataaaccaga tacacgtttt gaaatggaat
     6421 taattgacgt ttctcaatta ggacgtgata tggactttaa agtatttaaa gatactgttg
     6481 aaaatgatgg tgaaattaaa gcaattgtcg ctaaaggtgc agctgaacaa tatactcgta
     6541 aagatatgga tgctttaaca gaatttgtaa acatctatgg tgctaaagga ttagcgtggg
     6601 ttaaagttgt ggaagatggt ttgacaggtc caattggacg tttctttgaa acagaaaatg
     6661 ttgaaacatt acttacatta actggtgctg aagctggtga cttagtaatg tttgttgcag
     6721 acaaaccaaa tgtcgttgca caaagtttag gtgcattacg tgtcaaatta gctaaagaat
     6781 taggtttaat cgatgaaaca aaattaaact tcttatgggt gacagattgg ccattattag
     6841 aatatgatga agatgcgaaa cgttacgttg cagcacatca tccatttaca tctccaaaag
     6901 aagctgatat tgctaagctt ggcactgcgc cagaagaagc tgaggcaaat gcttatgaca
     6961 tagtattaaa tggttatgaa ttaggtggcg gttcaatcag aattcatgat ggtgagttac
     7021 aagaaaaaat gttcgaagtt cttggattta ctaaagaaca agcacaagaa cagttcggct
     7081 tcttactaga tgcatttaaa tatggtgcac caccacatgg cggtattgca ttaggattag
     7141 accgattagt catgttattg actaatagaa caaacttgag agatacaatc gcattcccta
     7201 aaacagcatc tgctacatgt ttattaacga atgcgcctgg tgaagtttct gataaacaat
     7261 tagaagaact ttctttgcga attcgtcact aaattttaaa aaatcaataa actttggttt
     7321 gttataattt cattaatttt aattatgtgg taatataaca tcataagata gtcatctgag
     7381 ttgttcgtaa gtttgctttt taatttgggc ctaacactct ttgatcaagg gagcccaatg
     7441 ggttttcttg cagcgtacac gccttatatg aaggacgtac aaaacaagaa acagggcacc
     7501 cacctgtata taacaggccg aatgatcaag ctattataac tacggcaata cggactctat
     7561 cgatacgcaa gactttgtct tgcgtatttt tatgcgtttc tttaagtttt taataaagaa
     7621 ttagttgggt tttatagcgt ttaataaatg tttcctcgtt gaattttggg aaaataatca
     7681 aagcgataat gctaaatata aaattttaag ttactatgta cttatgtata aatcagtgaa
     7741 agaggtaaaa taatgaaaca tcaattttca agaaatgaac tagcgattgg acaacaaggt
     7801 ttagatttat tgaagcaaca aactgttgtc atacttggcg ttggaggtgt tggttcattt
     7861 gctgctgaag cattagcaag aacgaatatt ggtcatatta tattaataga taaagatgat
     7921 gttgatatta caaatgtaaa cagacaatta cacgctttaa ctacaacggt aggacaaagt
     7981 aaagtttctt tgatggaaga acgtattaaa ttaattaatc cagattgtaa agtcacgccc
     8041 ttacatatgt tttatacaga agagacatat gaagaaattt tcgataacta cgatatagat
     8101 tattttgtag atgcaagtga tacaataatg tacaaagtac acttaatgaa agagtgtctt
     8161 gaccgaggga ttaaagtgat ttccagtatg ggtgctgcaa ataaaacaga tccaacacgt
     8221 tttacaattt cagatatctc taaaacacat accgatccaa tggccaaaat tattcgtcaa
     8281 aaattgaaaa aactaggaat tcgtaaaggg attccagttg tattttcaga tgaaagtcca
     8341 attgtcataa gagaagatgt aaaagatata gttggagata aaaatgctat caatcgaaaa
     8401 ggacaaatgc caccttcttc aaatgccttt gtgccaagtg ttgttggatt aatttgtgct
     8461 agttatgtgg tgaatgacgt attaaaagat attccagttc gtcgcattaa agacaaaggg
     8521 caataattca ttttgaaagt cataattttc aacgtaaggc aagtgtaacc acaccataaa
     8581 actaaaactg actagttcgc aagcatagta gaatatgcct cgtgtactag tcagttttga
     8641 tttgattata gctaaaatat caaaggtaat cgatttaatc ttatttttac ggcctttgtt
     8701 taagtaagtt gttatatatt tctttgagtt gttgttcact tttagatgtc gtttttggtt
     8761 cataataaat tttgttttta agtttatcag gtaaatattg ctgtgaaaca tagccattaa
     8821 catattggtg tggatatttg taaccaatag atcggcctag atctttagca ccttgataat
     8881 gtccatcttt taaatgattt ggaatttgac ccacatgacc gtttctaata tcggacaatg
     8941 cactatcaat ggcactcatt gctgaatttg acttaggtga taagcatagt tcgattactg
     9001 cttggcttag tggaattcta gcttctggta aacctagacg ttctgctgat tcaatagcag
     9061 caagtgttct ctgaccagca ttaggtgagg ctaagcctat atcctcatag ctaattacaa
     9121 gtaatcgtcg aactattgta ggtaaatctc cagcttcaat taatcgtgct aaataatgta
     9181 aagcggcatt gacgtcgcta ccacggatag atttttggaa agcgctcata acatcgtaat
     9241 gcatgtcacc atccttatca cttacaaatg cacctttttg taaacagtct ttagcatctt
     9301 gcaatgtaac atgtcgataa ccgtctttgt cattatctgc gcttaataca gctaattcca
     9361 atgcatttaa cgcactacga acatcacctt ggctttgtgt agaaaagtag gtcatggcat
     9421 cttcatcaat tttgggttga tatgttttca aaccattctc atcatcttct attgcacgag
     9481 ttaacgcttg gcgcacatct tcgtcattta aaggatataa ctcgaaaatt tgcgctcttg
     9541 aacgaatcgc tggattgata gcatgataag gatttgaagt tgtagcacca atcaagacga
     9601 ttttgccatt ttctaaatga ggtaataaaa agtcttgttt agctttatct agtcgatgta
     9661 tttcatctaa caacaagata acttgaccag acattttagc ttcttcaaca acaagttgca
     9721 tatctttttt agtgttagtt acagcattca attgtctgaa tttatattgc gtactgcccg
     9781 aaattgcttt ggcaatactt gttttgccta taccaggtgg accataaaaa atcattgaag
     9841 ttaatttttt tgtgtcaacc attcttctga taatgccttt tggtccaact aaatgttgtt
     9901 gggaaatgat ttcatctata ttttttgggc gcattctcga tgctaatggt tctgtactca
     9961 catttgtcac acctttctta tactatctta actcaaaaaa tgctaaaatg ggaatatcaa
    10021 tattcgaatt aagtgaggtt atacaatgaa aatttctact aaagggagat atggacttac
    10081 attgatgatt tctcttgcta aaaaagaggg gcaaggatgt atatcattaa agtcaattgc
    10141 tgaagaaaat aatttgagtg atttatattt agaacagctt gtaggtcctt taagaaatgc
    10201 ggggttaatt cgaagtgtac gcggtgctaa aggtggatac caattaagag taccagcgga
    10261 agaaatctca gcaggggata ttataagact gttagaaggt ccaattacat ttgttgaaag
    10321 tattgaatca gaaccacctg cgcaaaaaca actatggatt cgcatgagag atgcagtgag
    10381 agatgtttta gataatacaa cattgaaata tttagcggaa tatgtagata caagtgaaga
    10441 tttagacgga tacatgtttt atatttaatc gtataaatac tatactataa cataaaaact
    10501 tcatattata atgtttagcg aacctcctta gtggtatata aatatataca tccaaggagg
    10561 tttcactatg gcagacgaaa gtaagttcga tcaatttaaa ggaaatgtta aagaaacagt
    10621 aggtaacgtt actgataaca aagaattaga aaaagaaggt cagcaagata aagcgactgg
    10681 taaagcaaaa gaagttgttg aaaatgctaa aaacaaaata actgatgcaa ttgataaact
    10741 taaaaaataa gttcaattaa actaatcaaa tataagagga gcgattaata tgagttttat
    10801 ggataaagct aaagacgcag tagaaa
//
LOCUS       DAHHSC010000073        10780 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.073, whole genome shotgun sequence.
ACCESSION   DAHHSC010000073 DAHHSC010000000
VERSION     DAHHSC010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 10780)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10780)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10780
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.073"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            complement(89..177)
                     /locus_tag="NBI57_001429"
     tRNA            complement(89..177)
                     /locus_tag="NBI57_001429"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(140..142),aa:Ser,seq:gga)
     gene            complement(232..303)
                     /locus_tag="NBI57_001430"
     tRNA            complement(232..303)
                     /locus_tag="NBI57_001430"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(268..270),aa:Glu,seq:ttc)
     gene            complement(305..379)
                     /locus_tag="NBI57_001431"
     tRNA            complement(305..379)
                     /locus_tag="NBI57_001431"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(345..347),aa:Asn,seq:gtt)
     gene            complement(397..470)
                     /locus_tag="NBI57_001432"
     tRNA            complement(397..470)
                     /locus_tag="NBI57_001432"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(436..438),aa:Gly,seq:tcc)
     gene            complement(486..561)
                     /locus_tag="NBI57_001433"
     tRNA            complement(486..561)
                     /locus_tag="NBI57_001433"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(526..528),aa:His,seq:gtg)
     gene            complement(575..647)
                     /locus_tag="NBI57_001434"
     tRNA            complement(575..647)
                     /locus_tag="NBI57_001434"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(612..614),aa:Phe,seq:gaa)
     gene            complement(664..739)
                     /locus_tag="NBI57_001435"
     tRNA            complement(664..739)
                     /locus_tag="NBI57_001435"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(704..706),aa:Asp,seq:gtc)
     gene            complement(761..834)
                     /locus_tag="NBI57_001436"
     tRNA            complement(761..834)
                     /locus_tag="NBI57_001436"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(798..800),aa:Met,seq:cat)
     gene            complement(1235..1789)
                     /locus_tag="NBI57_001437"
     CDS             complement(1235..1789)
                     /locus_tag="NBI57_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase family protein"
                     /protein_id="HCD1822381.1"
                     /translation="MTDVIIVHSMHGNSRNHWYQWLEHNLTLEGYDVTLFNFESPEAK
                     TVDQWIEAMTKQINVRKKDTYFVTHGLGSITALKYIEMIDQPIEGFFSIAGFKEDAEN
                     IDLDIDLSNVTIDYDNIKKKVDNFYGLSSKNDKYVSYKETQRLMNALEGNLRIVEDGG
                     HFLEEDGFETFTALQDRMQDYMTR"
     gene            complement(2112..3512)
                     /locus_tag="NBI57_001438"
     CDS             complement(2112..3512)
                     /locus_tag="NBI57_001438"
                     /EC_number="1.3.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoporphyrinogen oxidase"
                     /protein_id="HCD1822382.1"
                     /translation="MTKSVAIIGAGITGLSSAYFLKQQDPNIDVTIFEASNRPGGKIQ
                     SYRKDGYMIELGPESYLGRKTIMTELAKDIGLEQDIVTNTTGQSYIFAKNKLYPIPGG
                     SIMGIPTDIKPFVTTKLISPLGKLRAGLDLIKKPIQMQDGDISVGAFFRARLGNEVLE
                     NLIEPLMGGIYGTDIDKLSLMSTFPNFKEKEEAFGSLIKGMKDEKNKRLKQRQLYPGA
                     PKGQFKQFKHGLSSFIEALEQDVKNKGVTIRYNTSVDDIITSQKQYKIVYSNQQEDVF
                     DGVLVTTPHQVFLNWFGQDPAFDYFKTMDSTTVATVVLAFDEKDIENTYDGTGFVIAR
                     TSDTDITACTWTSKKWPFTTPEGKVLIRAYVGKPGDTVVDDHTDNELVSIVRRDLSQM
                     MTFKGDPEFTIVNRLPKSMPQYHVGHIQQIRQIQAHIKQTYPRLRVTGASFEAVGLPD
                     CITQGKVAAEEVIAEL"
     gene            complement(3536..4459)
                     /gene="hemH"
                     /locus_tag="NBI57_001439"
     CDS             complement(3536..4459)
                     /gene="hemH"
                     /locus_tag="NBI57_001439"
                     /EC_number="4.99.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HCD1822383.1"
                     /translation="MTKKMGLLVMAYGTPYKESDIEPYYTDIRHGKRPSEEELQDLKD
                     RYEFIGGLSPLAGTTDDQADALVSALNKAYADVEFKLYLGLKHISPFIEDAVEQMHND
                     GITEAITVVLAPHYSSFSVGSYDKRADEEAAKYGIQLTHVKHYYEQPKFIEYWTNKVN
                     ETLAQIPEEEHKDTVLVVSAHSLPKGLIEKNNDPYPQELEHTALLIKEQSNIEHIAIG
                     WQSEGNTGTPWLGPDVQDLTRDLYEKHQYKNFIYTPVGFVCEHLEVLYDNDYECKVVC
                     DDIGANYYRPKMPNTHPLFIGAIVDEIKSIF"
     gene            complement(4517..5554)
                     /gene="hemE"
                     /locus_tag="NBI57_001440"
     CDS             complement(4517..5554)
                     /gene="hemE"
                     /locus_tag="NBI57_001440"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HCD1822384.1"
                     /translation="MVHNKNNTILKMIKGEETSHTPVWFMRQAGRSQPEYRKLKEKYS
                     LFDITHQPELCAYVTHLPVDNYHTDAAILYKDIMTPLKPIGVDVEIKSGIGPVIHNPI
                     KTIQDVEKLSQIDPERDVPYVLDTIKLLTEEKLNVPLIGFTGAPFTLASYMIEGGPSK
                     NYNFTKAMMYRDEATWFALMNHLVDVSVKYVTAQVEAGAELIQIFDSWVGALNVEDYR
                     RYIKPHMIRLISEVKEKHDVPVILFGVGASHLINEWNDLPIDVLGLDWRTSINQAQQL
                     GVTKTLQGNLDPSILLAPWNVIEERLKPILDQGMENGKHIFNLGHGVFPEVQPETLRK
                     VSEFVHTYTQR"
     gene            complement(5544..5654)
                     /locus_tag="NBI57_001441"
     CDS             complement(5544..5654)
                     /locus_tag="NBI57_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1822385.1"
                     /translation="MIKCQFYDIYLLKIRTNERYDNIDKNDFNLGGLYGA"
     gene            5817..6320
                     /gene="traP"
                     /locus_tag="NBI57_001442"
     CDS             5817..6320
                     /gene="traP"
                     /locus_tag="NBI57_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction protein TRAP"
                     /protein_id="HCD1822386.1"
                     /translation="MKKLYTSYGTYGFLHQIKINNPTHQLFQFSASDTSVIFEETDGE
                     TVLKSPSIYEVIKEIGEFSEHHFYCAIFIPSTEDHAYQLEKKLISVDDNFRNFGGFKS
                     YRLLRPAKGTTYKIYFGFADRHAYEDFKQSDAFNDHFSKDALSHYFGSSGQHSSYFER
                     YLYPIKE"
     gene            complement(6444..7667)
                     /locus_tag="NBI57_001443"
     CDS             complement(6444..7667)
                     /locus_tag="NBI57_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCD1822387.1"
                     /translation="MHNQATTLFNKRLHALRKEKNYYNKFIFNGHFMVFLLILLGAFI
                     FGYGEWLKHIPTNINFALIAAVIVALTSIFPMRPLLKEADKIFLLPFEKHMSQFMRHA
                     ILYSYFARILIQLIIVIVMFPLFYNINQHNVAFYICFGFSALIFPYVGLRLRWQWYQS
                     GLKTWQVNLISFIIFALTYYLLLAPKWYIVFVMVALPVLIEFLVKKYKPGFLYPWEKM
                     IAIEHRHHMNYYKFVNMFTDVKHLKESAVRRSYLDILLPVPKGSKFNSNAMYLFLFIR
                     SFIRGRDAFNIIFRLVIIAVLLMVWLSYPLVTGVIGSLFVYIILLQMAQFYSQQAYGL
                     WPQVWPVPEEKVIKGYEQFLYRLMFVICTVFAVTFIIKHMTLFYVVLIFYIVGLLTIR
                     SIIKKLKYQETLLRD"
     gene            complement(7660..8400)
                     /locus_tag="NBI57_001444"
     CDS             complement(7660..8400)
                     /locus_tag="NBI57_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCD1822388.1"
                     /translation="MTVKVEQLTGGYGKRPVIKDINFELNKGEIVGLIGLNGAGKSTT
                     IKHMLGLLTPMEGSLSISDININDDIEAYRRKLSYIPESPVIYEELTLEEHIEMTAMA
                     YDIDRDEAMNRAMPLLKTFRLENELKVFPSHFSKGMKQKVMIICAFIVNPELYIIDEP
                     FLGLDPLGIQSMLDLMVEKKNEGRTVLMSTHILATAERYCDRFIILDEGEVVAFGDLE
                     ALRQQTGLHNQTLDDIYIHVTQGGDVHA"
     gene            8534..8956
                     /locus_tag="NBI57_001445"
     CDS             8534..8956
                     /locus_tag="NBI57_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HCD1822389.1"
                     /translation="MSETIFGKILTGEIPSFKVYEDDYVYAFLDISQVTKGHTLLIPK
                     KASANIFETDEETMKHIGAALPKVANAIKRAFNPDGLNIIQNNGEFADQSVFHIHFHL
                     IPRYENDIDGFGYKWETHEDILDNDAKQQIAEQIQAQF"
     gene            9098..9463
                     /locus_tag="NBI57_001446"
     CDS             9098..9463
                     /locus_tag="NBI57_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HCD1822390.1"
                     /translation="MKASRILFGIGVGVAAGFVVALQGRDDKSVKNNTIDRTAPTGSK
                     SELQREFETIKQSFNDILNYGVQIKNESAEFGSSIGGEIKSLLGNFKSDINPNIERLQ
                     SHIENLQNRGEDIGNEISK"
     gene            10198..10755
                     /locus_tag="NBI57_001447"
     CDS             10198..10755
                     /locus_tag="NBI57_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3267 domain-containing protein"
                     /protein_id="HCD1822391.1"
                     /translation="MFLCKRQIDINARFGLPRIAFMSAVATIIMFLVSYEVMYFLSNT
                     PLSDRHFLIFLLLVFMTYPLHKSIHLLFFLPYRKSFKVHKLTKRKWLIFYNTYVNQPV
                     HKFYFCINLILPLIILSAMFVYLTISFPQYGHYFMFLLALNFGISITDLLYLKIIIFS
                     NYGQYIEEHSTGINILKKIKNPYHL"
BASE COUNT         3530 a         1993 c         1550 g         3707 t
ORIGIN      
        1 acaaatttta aattaagtga ctcagataat agacaaaaaa ccttaaaaat agcgcttaaa
       61 atgggtaaaa ccattttaag cgctataacg gagagtgagg gattcgaacc ctcgagacgc
      121 ttgtggcgcc tacacacttt ccaggcgtgc tccttcggcc aactcggaca actctcctca
      181 ataataaata tgtaataaaa aaacagaagc gatatttcac ttctgtttgt atgactccta
      241 cgggactcga acccgtgtta ccgccgtgaa agggcggtgt cttaaccgct tgaccaagga
      301 gccatggctc cacaggtagg actcgaacct acgaccgatc ggttaacagc cgatagctct
      361 accactgagc tactgtggaa taataataga aaataatgga gcgggtgatg ggaatcgaac
      421 ccacaacatc agcttggaag gctgaggttt tgccattaaa ctacacccgc taatgtaaac
      481 gattatgggg cggctgaagg ggatcgaacc ctcgaatgtc ggaaccacaa tccgatgtgt
      541 taaccacttc accacaaccg ccatgaatta agaatggttc aggacagagt cgaactgccg
      601 acacatggag cttcaatcca ttgctctacc aactgagcta ctgaaccgta atggtaataa
      661 taatggcggt ctcgacggga atcgaacccg cgatctcctg cgtgacaggc aggcgtgtta
      721 accgctacac tacgagacct attaaattaa aaactatgta ttgcgggagg cggatttgaa
      781 ccaccgacct tcgggttatg agcccgacga gctaccgaac tgctccatcc cgcgctaata
      841 ttattttgaa ttacctaatt aatataccat atttaaaaac ctaaagtcaa gaactttttt
      901 aatttaattt aaatattatt tcttaaataa ttacttaaat atcgtagcaa catgttctct
      961 gttgaacaca aatattagta tattcatttt tgtagtgtac gtcaacgaca ttttgaaagt
     1021 tttttgtgta aaaaaacgct tcttcttccc ttttataata taagtgtcta atagttgtca
     1081 taattactca tgaactattg aaaaagatgt aggaatcata aattttataa tgcctacacc
     1141 tctaaactat taaaatataa ttataatctt tttcactaaa gtgctatgct tcgtactgac
     1201 acacataaca caatacatag caaccaaaca ttacctatcg tgtcatgtaa tcttgcatcc
     1261 gatcttgcaa cgctgtaaat gtttcgaagc catcctcttc taagaagtgc cctccatctt
     1321 ccacgattcg caagttcccc tctaatgcat tcattaaacg ctgggtttct ttatatgaaa
     1381 cgtatttgtc atttttagaa ctcaatccgt aaaaattgtc aactttcttt ttaatattat
     1441 cgtaatcaat ggttacatta cttaaatcaa tatctaaatc tatattttct gcatcttctt
     1501 taaagcccgc tatactaaaa aagccttcaa tcggctgatc aatcatttca atatatttta
     1561 aagctgtgat tgaacctaaa ccatgtgtta caaaatatgt atcctttttg cgtacattaa
     1621 tttgtttcgt catagcttca atccactgat ccactgtctt cgcttcaggg gattcaaaat
     1681 taaataatgt tacgtcatat ccttctaaag ttaagttatg ctccaaccac tgataccaat
     1741 gatttctact atttccatgc atagaatgta caataattac atctgtcatc tcattctctc
     1801 ctttcaactt actacttctt ttctattttt aaaaaaatga ctgattacct ataattgtaa
     1861 aataaaaaca ccttaattag aaatgttata tcgcaaagtg acatttctaa ttaaagtgta
     1921 ttgtcatcat ttcaatatca ttcaaaaaca gctaaacctt tgtctctgct tcaatttcac
     1981 aaaaataatt cccgctgaaa gtatctatat ttacacatta cttccaccat tatataactt
     2041 aaaaatgact atatttcatc aaacattatc taaagacgtc gcacctacac caacaccatc
     2101 caacaattaa cttacaactc tgcgattact tcttcagcag caactttacc ttgtgtaata
     2161 caatcaggta gtccaaccgc ttcaaaagat gcaccagtta ctctaagtcg tggatatgtt
     2221 tgtttaatat gtgcttgaat ctgtctaatt tgttgaatat gaccgacatg gtactgtggc
     2281 atacttttcg gcaaacgatt gacaattgta aattcaggat cacctttaaa tgtcatcatt
     2341 tgacttaaat ctctacgtac aatcgatact aattcattat ctgtatgatc atcaaccaca
     2401 gtatcacctg gtttacctac atacgcacga atcaaaacct taccttctgg tgtagtaaat
     2461 ggccattttt tcgatgtcca agtacatgcg gtaatgtctg tatcactcgt tctcgcaatc
     2521 acgaagccag taccatcata agtattttca atgtcttttt catcaaatgc caatacaaca
     2581 gttgcaacag tcgtactatc catcgtttta aagtaatcaa atgctggatc ttgtccgaac
     2641 caattcaaaa agacttgatg cggtgttgtc actaataccc catcgaatac atcttcttgt
     2701 tgattactgt aaacaatttt atattgcttt tgagatgtaa taatatcatc cactgacgta
     2761 ttgtagcgta ttgtcacacc tttatttttc acatcttgtt ctaatgcttc aataaatgag
     2821 cttaaaccat gcttaaattg tttgaattgt ccttttggtg cgccaggata taattgtctt
     2881 tgtttcagac gcttattttt ctcatccttc atacctttta tcagacttcc gaatgcctct
     2941 tctttttctt taaaattagg aaacgtactc atcaaactta atttatcaat atcggtacca
     3001 taaataccac ccattaaagg ctcaattaag ttctcaagta cctcattacc taatcttgct
     3061 ctgaaaaatg caccaacaga aatgtcacca tcttgcattt gtataggctt tttgattaaa
     3121 tctaatcctg ctcttaattt accaagtggc gatattaatt ttgtagtaac aaatggttta
     3181 atatctgttg gaatacccat aattgaacca cctggaatcg gatataattt atttttcgca
     3241 aaaatatatg attgtccagt cgtatttgta acaatatctt gttctaatcc aatatctttc
     3301 gctaattctg tcataatcgt ttttctacct aaataagatt caggccctag ttcaatcata
     3361 taaccatctt tacgatacga ttgaatcttt ccccccggac gattcgatgc ttcaaagatg
     3421 gttacatcaa tattaggatc ttgctgtttt aaaaaatatg cacttgataa acctgttatc
     3481 cccgctccta taatagccac tgatttagtc acaacgtttc acgcttcttt cgtcattaaa
     3541 atatagactt gatttcatca acaattgcac cgataaataa tggatgtgta ttcggcattt
     3601 ttggacgata ataattcgca ccaatatcat cgcaaacaac tttacattca taatcattgt
     3661 cataaagcac ctctaaatgc tcacatacaa aacctactgg cgtatatata aagtttttat
     3721 actgatgttt ttcatataaa tcacgtgtta aatcttgtac atctggccct aaccaaggtg
     3781 tacctgtatt accttcagat tgccaaccaa tcgcgatatg ttcaatatta gattgttctt
     3841 taattaaaag cgcagtatgt tctagttctt gtggatatgg atcattattc ttttcgatta
     3901 aaccttttgg caaactatgt gccgaaacaa ctaataccgt gtctttatgt tcctcttccg
     3961 gtatttgagc taatgtttcg ttgactttat tcgtccaata ttcaataaat ttaggttgtt
     4021 cataataatg tttcacatgt gtaagttgaa taccatattt tgcagcttct tcatcagcac
     4081 gtttgtcata tgatcctact gaaaatgaag aataatgtgg tgctagtact accgtgattg
     4141 cttcagtaat accatcattg tgcatttgtt caaccgcatc ttcgataaat ggtgaaatgt
     4201 gttttaatcc taagtatagt ttaaattcaa catctgcata tgctttattt aatgctgaaa
     4261 ctagtgcatc agcttggtca tctgttgtac ctgctaatgg tgataaacca cctataaatt
     4321 catatctatc tttcaaatct tgaagttctt cttcagatgg acgtttacca tgtctaatat
     4381 ctgtataata tggctctatg tcactttctt tataaggtgt gccataagcc ataactaata
     4441 accccatttt tttagtcatt gataatacct tcctttaaat gaattatctt tcatgtgctt
     4501 caatgtaata ctatgattat ctttgtgtat atgtgtgtac gaattcgctt actttacgta
     4561 acgtctctgg ttgcacttct gggaaaacac cgtgtcctaa attaaagatg tgtttaccgt
     4621 tctccatacc ttgatctaat attggtttca atctctcttc aatgacattc catggtgcta
     4681 ataaaattga tggatctaaa ttcccttgta atgttttagt aacgcctaat tgttgagcct
     4741 gattaataga cgttctccaa tctaggccta atacatcaat cggtaaatca ttccattcat
     4801 tgattaaatg actggcacct acaccgaata aaattaccgg cacatcatgt ttttctttaa
     4861 cctcactgat taatcgaatc atatgtggtt taatgtaacg tctgtaatcc tcgacattta
     4921 atgcacctac ccatgaatcg aaaatttgaa tcaattcggc acctgcttcg acttgagctg
     4981 ttacatattt aacagataca tcaactaaat gattcattaa agcaaaccat gttgcttcat
     5041 ctctatacat catcgctttt gtaaaattgt aatttttcga tggtccgcct tcaatcatat
     5101 atgacgctaa tgtaaatggt gccccagtaa atcctattag cggcacattt aacttttctt
     5161 ctgttaaaag tttaattgta tctaatacat atggtacatc tcgttcgggg tctatttgag
     5221 aaagtttctc aacatcttga attgttttga taggattatg aatcactgga ccaatacccg
     5281 atttaatttc tacatcgaca ccaattggct ttaatggtgt cataatatct ttgtataaaa
     5341 ttgctgcatc tgtatgataa ttatcaactg gtaaatgtgt tacataagcg cacaactccg
     5401 gctgatgtgt aatatcgaat agtgaatatt tttctttcaa ttttcgatat tctggttgcg
     5461 aacggccagc ttgtcgcata aaccaaacag gtgtatgtga tgtttcttca cctttgatca
     5521 tttttaaaat tgtattgttt ttattatgca ccataaaggc ctcctaaatt aaaatcattc
     5581 ttatctatat tatcatatcg ctcattcgtt cgtattttca ataaataaat gtcataaaac
     5641 tgacatttaa tcatagaact atttattgta aatttaaatt ctaaagtcca ttattttgta
     5701 tcattacttc taaatatctc gcaagattca ttatagtaat tttaatcaat tattaatagt
     5761 ggtaatgact agtttatcat cgtataataa ataaaaacat aagggggacc tttcatatga
     5821 agaaactata tacatcttat ggcacttatg gatttttaca tcaaataaaa atcaataacc
     5881 cgacccatca actattccaa ttttcagcat cagatacttc agttattttt gaagaaactg
     5941 atggtgagac tgttttaaaa tcaccttcaa tatatgaagt tattaaagaa attggtgaat
     6001 tcagtgaaca tcatttctat tgtgcaatct tcattccttc aacagaagat catgcatatc
     6061 aacttgaaaa gaaactgatt agtgtagacg ataatttcag aaactttggt ggctttaaaa
     6121 gctatcgttt gttaagacct gctaaaggta caacatataa aatttatttc ggatttgctg
     6181 atcgacatgc atacgaagac tttaagcaat ctgatgcctt taatgaccat ttttcaaaag
     6241 acgcattaag tcattacttt ggttcaagcg gacaacattc aagttatttt gaaagatatc
     6301 tatacccaat aaaagaatag actcgaagaa aacttccatt ggttatgctg ttaacgtata
     6361 gatgctaatg taatctgaac gccgtatcat ttggcgttca acattacatg cacctttaca
     6421 acatacatgc caatggaagt tttttagtct cgtaataatg tttcctgata tttcaacttt
     6481 ttaataatac ttctaatcgt taataaacct acaatataga aaatgagtac aacatagaat
     6541 aacgtcatat gttttataat aaatgtcact gcaaacacag tacaaataac aaacattaat
     6601 ctatataaaa actgttcata ccctttgatt accttctctt caggtacagg ccacacttga
     6661 ggccataagc catatgcttg ttgtgaataa aattgtgcca tttgcaacaa tataatatac
     6721 acaaaaagac ttccaataac ccctgtcact aatggatatg ataaccaaac cataagtaaa
     6781 actgcaataa ttactaacct aaagataata ttaaatgcgt ctctccctct tataaagctt
     6841 ctaataaata agaataaata catcgcatta gagttaaatt tactaccctt tggaactggt
     6901 aaaagtatat ctagataact tcttctgact gcagattctt tcaaatgttt tacatcggtg
     6961 aacatattaa caaatttata ataattcata tgatgtcgat gttcgatagc aatcattttc
     7021 tcccaaggat acaaaaagcc tggtttatat tttttaacta aaaattctat taacacaggc
     7081 aaagcaacca tcacaaatac gatgtaccat tttggagcta atagtaagta atatgttaga
     7141 gcaaagatga taaatgatat taaattaact tgccatgttt taagtcccga ttgataccat
     7201 tgccatctta agcgtaaacc aacatatgga aaaattaatg cactgaatcc aaaacaaata
     7261 taaaatgcca cattatgttg attaatattg taaaacaacg ggaacattac aataacaata
     7321 atgagttgga ttaatatgcg cgcaaagtaa ctatataaaa tcgcatggcg cataaattga
     7381 gacatgtgtt tttcaaatgg taataaaaag attttatccg cttcttttag gagtggtcgc
     7441 attggaaaaa tggatgtcaa cgcaacaatc actgctgcta ttaatgcaaa attgatattc
     7501 gttggaatat gttttaacca ttcaccatat ccaaaaataa atgcacccag caaaataagt
     7561 aaaaagacca tgaaatgacc attaaatata aacttattat aataattttt ctctttacga
     7621 agggcatgta atcttttatt aaataatgtg gtcgcttggt tatgcatgta catctccacc
     7681 ttgcgtcaca tgaatatata tatcgtctaa tgtttgatta tgtaagccag tttgttgtct
     7741 caatgcttct aaatctccaa atgcaacgac ttcaccttcg tctagtatga taaaacgatc
     7801 acaataacgt tcagctgttg ctaaaatatg tgtactcatt agaacggttc taccttcgtt
     7861 tttcttttca accattaaat ctaacatgga ttgaattcct aatggatcta gtccaaggaa
     7921 tggttcgtct ataatataca attcgggatt aacgataaac gcacaaataa tcatgacttt
     7981 ttgtttcatc cccttagaaa aatgactcgg aaaaactttc aattcatttt ctaaacggaa
     8041 tgtctttaat aatggcattg ctcgattcat cgcttcatca cgatcaatat catatgccat
     8101 tgctgtcatc tctatatgtt cctctaatgt gagttcttca taaataaccg gtgattccgg
     8161 aatataagat aactttcttc tatatgcctc tatgtcatca ttaatgttga tatctgaaat
     8221 tgatagagat ccttccatag gtgtaagcaa tcctagcata tgtttaatcg ttgtactctt
     8281 accagcgcca ttaaggccaa taagtccaac gatttcgcct ttgtttaatt caaaatttat
     8341 atctttaatt acagggcgtt ttccatatcc acctgtaagc tgttctactt taactgtcat
     8401 aaggcacctc catgacttat attgtaccaa aaattataaa atgctcatat taaatacaca
     8461 tgtcctaata tcgaattttt agcgacaatg ttataatgaa tggtaatact agttgaaaag
     8521 gagtgtagtc atcatgtcag aaacaatttt cggcaaaatt ttaactggag aaattcctag
     8581 ctttaaagta tatgaagacg attatgtcta tgccttttta gatatatcac aagttactaa
     8641 aggacatacg ttattaattc ctaaaaaagc ttctgctaat atctttgaaa ctgatgaaga
     8701 aacaatgaaa catatcggtg cagcattacc taaagtagca aatgctatta agcgtgcatt
     8761 taatcctgat ggtttaaaca ttattcaaaa taatggtgag tttgcagatc aatctgtatt
     8821 tcatattcat ttccacttaa ttcctcgata cgaaaatgat attgatggat ttggttataa
     8881 gtgggaaaca catgaagaca ttttagataa cgatgcaaaa caacaaattg ctgaacaaat
     8941 tcaagcacaa ttttaaatgt atgcttaatc taagctcgaa cgggtataat atgattaata
     9001 ttataacaat tgcgtttgaa gtgataacat caaggttagc aattttaaac aaaatgagtt
     9061 atcaagataa cagatgttaa aagtgaggag aatataaatg aaagcatcac gcattctatt
     9121 cggtatcggt gttggcgtag cagctggttt tgtagttgca cttcaaggac gagacgacaa
     9181 aagtgtcaag aacaacacga tcgatcgtac tgcccctact ggttcaaaat cagaactaca
     9241 acgtgaattt gaaacgatta aacaaagttt taatgacatt ttaaactatg gtgttcaaat
     9301 taaaaacgaa agtgcggaat ttggtagttc aattggtggt gaaattaagt cattacttgg
     9361 aaacttcaaa tctgacatta atcctaatat tgaacgttta cagtcacaca tcgaaaattt
     9421 acaaaatcgt ggcgaggata ttggaaacga aatttctaag tagcacgtta cgttctcgat
     9481 cacaactatt tttattagta acagcatatt tattttttta aattaaatgc caaataaacg
     9541 agatgacatt agaaattaga tatttcttgt catctctttt ttataactca aatgaactta
     9601 tgtttacaaa ttacaggaat acatcgtttg tagtgatttt cgcttaaatc atatttatga
     9661 attgattgaa aacattgctt aggattcatt gtgttatcct tgcactttga ttacgcttta
     9721 cttatatcat tatcgacaaa caacatactt atattttcat tgagccgaac cttatataca
     9781 cattacatat accttacttg cacaaattat taatctggta tttattataa ttacatatca
     9841 ctatattttt agcatttgta taacttagtt ggtcaaaaga tgcttttgca tatgccctgc
     9901 catatccatt ttttatatta ttcaagtttt caaatactca tgtcacacgc gcctttttca
     9961 taagtgatgc tttattagca agaagatgtg ttcgcagaaa tttgttctgc attctacttc
    10021 tacgctagtc aatcagccaa ttttaccaat ctccactttc gcgtttcaaa tcaaacaata
    10081 cgtgtctccc ctttcttctt atataacaat tcttctaaca tgatatgtta ctattgaatt
    10141 actgaacctg agttagttat aatctaactt atattgaaaa gagatgaggc gtaagatatg
    10201 tttttatgta aaagacaaat tgatatcaat gcacgatttg gtttgcctag aattgcattt
    10261 atgagtgcag ttgcaaccat cattatgttt ttagttagtt atgaagtaat gtatttttta
    10321 tctaatacgc cattatcaga tagacatttt ctcatctttt tattacttgt atttatgacg
    10381 tatccattac ataaaagtat acatttatta tttttcttac catatagaaa atcgtttaaa
    10441 gttcataagt taactaaaag aaaatggctt atattctata atacctacgt caatcaacct
    10501 gtacacaaat tttatttttg cattaactta atattgccgt taattatctt atctgcaatg
    10561 ttcgtttatc taacaatttc attcccgcaa tatggacatt attttatgtt cttattggca
    10621 ttgaatttcg gtatttccat tacagattta ttatatttaa aaataattat attttctaat
    10681 tatggacaat atatagaaga acatagtaca ggtattaata ttttgaaaaa aattaaaaat
    10741 ccatatcatt tataacaaaa taattatagc aaggtgttat
//
LOCUS       DAHHSC010000074        10743 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.074, whole genome shotgun sequence.
ACCESSION   DAHHSC010000074 DAHHSC010000000
VERSION     DAHHSC010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 10743)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10743)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10743
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.074"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            <1..608
                     /locus_tag="NBI57_001448"
     CDS             <1..608
                     /locus_tag="NBI57_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1361 domain-containing protein"
                     /protein_id="HCD1822392.1"
                     /translation="YIARIYFLILFIVSLFETKIFQFMTLNLFLAYIPFELSLLLRLF
                     KPNKKYEWPLFIIYSFIFVLLLPNTFYMVTDLIHLNQFQFNFYAGLNLSEWKYFTYLL
                     LGVFLAVYVMILIYFEILTLTSHRWLNRLIVVVLMFLNGFGIYIGRFLRLHSVYFFNE
                     PLHVLNHILESLTLKTAMFVCFMVVMQAAILLFGKGVRLNK"
     gene            605..889
                     /locus_tag="NBI57_001449"
     CDS             605..889
                     /locus_tag="NBI57_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000154460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1822393.1"
                     /translation="MTINIIVLIVSIIVFQLIIGHIWHDIGLSYLRSILLMMLPFGLG
                     VFIQQVSYYERQYPKWQVPQNIKVRLKYIYLATFLEYVVLYLTLFTDILR"
     gene            complement(1044..2417)
                     /locus_tag="NBI57_001450"
     CDS             complement(1044..2417)
                     /locus_tag="NBI57_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HCD1822394.1"
                     /translation="MAIAVLLNRMFRMEHNPLFEYIYQQKEDIDACYFIIPEEDMSSA
                     SDLKAQFYRGTLQRFYQSLHAEKLTPYVMSYDDIISFCKENNISEVVIAGDIMSYHLE
                     EYDILHQRSLFNEARIAVTLIRGNHYFKASKTMNQQGEPYKVFTSFYRKWRPYLRHRD
                     VYHYDLKSFEDFVIASPDDLVFDDIAFGSSQVIEQNKWQHFLDQDIQNYESGRDYLPE
                     VLTSQLSVALAYGLLDIIEIFNDLLARYDEDEANHEAFIRELIFREFYYVLMTQYPET
                     SYQAFKPKYRQIKWSQNEANFNAWCEGQTGFPIIDAAIMELTQTGFMHNRMRMVVSQF
                     LTKDLFIDWTWGEKFFRKHLIDYDAASNIHGWQWSASTGTDAVPYFRMFNPIRQSERF
                     DAKALYIKTYLPIFNQIDAKYLHDTQRNESNLFEQGIELGSHYPRQMVDHQEKRTQVL
                     ATFKALD"
     gene            complement(2503..4056)
                     /locus_tag="NBI57_001451"
     CDS             complement(2503..4056)
                     /locus_tag="NBI57_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DASS family sodium-coupled anion symporter"
                     /protein_id="HCD1822395.1"
                     /translation="MSEEKHVVEHEQQKKEKTKKQYKPFWIVMSFIILIVVLLLPAPS
                     SLPIMAKAVLAILAFAVIMWVTEAVSYPVSATLIIGLMILLLGFSPVQNLGEKLGNPK
                     SGSAILAGSDLLGTNHALSLAFSGYATSAVALVAAALFLAAAMQETNLHKRLALLVLS
                     IVGNKTRNIVIGAIIVSIVLAFFVPSATARAGAVVPILLGMIAAFKVSKDSKLASLLI
                     ITSVQAVSIWNIGIKTAAAQNIVAINFINHQLGFDVSWGEWFLYAAPWSIVMSVALYF
                     IMIKVMPPEINTIEGGKDLIKEELHKLGPVSPREWRLIVISMLLLLFWSTEKVLHPID
                     SASITIIALGVMLMPKIGVMTWKHVENKIPWGTIIVFGVGISLGNVLLKTGAAQWLSD
                     QTFGVLGLKHLPIIATIALITLFNILIHLGFASATSLSSALIPVFISLTSTLHLGDQS
                     IGFVLIQQFVISFGFLLPVSAPQNMLAYGTGTFTVKDFLKAGIPLTIVGYILVIVFSM
                     TYWKWLGLL"
     gene            complement(4339..4626)
                     /locus_tag="NBI57_001452"
     CDS             complement(4339..4626)
                     /locus_tag="NBI57_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1822396.1"
                     /translation="MTNEDKRFEQLRFERKFIVIPYLIYAVIVLLLNIFYSDLKITMT
                     LFGLFFAYNVVILFIAFIKHYKRTLLLSLILTVLSGAAFFGIIYVYGINHF"
     gene            complement(4661..5569)
                     /locus_tag="NBI57_001453"
     CDS             complement(4661..5569)
                     /locus_tag="NBI57_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HCD1822397.1"
                     /translation="MEQIMINHYVHFSRLVQGFWRANEWKMTAKELNYFINELVERGI
                     TTMDHADIYGDYQCESLFGNALDLSPELRNKIQIVTKCGIILPSKQFDFTNGHRYDLS
                     SKHIVKSVEQSLINLNVDYLDSLLIHRPSPLMDPEQVADALTKLVKQGKLKSFGVSNF
                     NHSQYQLLNQYIMKERLHISINQLELSPYHVDSLQDGTMDSMYQNHVQIMAWSPFAGG
                     KIFDKEDIKAQRIMKVVQSIADKYGVSDTAVMIAWLVKIPHSIMPILGTSQLKRIDQA
                     IEGLQLNLDDQSWFDIYTAIIGQDIP"
     gene            complement(5672..6598)
                     /locus_tag="NBI57_001454"
     CDS             complement(5672..6598)
                     /locus_tag="NBI57_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HCD1822398.1"
                     /translation="MKNNKDEKIRISIINGFLGSGKTTLLTHYISELLKNDEKIKIIM
                     NEFGTFDIDSNSISNEIEVHSLINGCVCCDLKQELVYELKAIALKGDVNHVIIEATGI
                     AHPLELLVACQDPQIVNFFEKPIIYGVLDATRFLERHQYTENTVSLMEDQLKLSDMII
                     INKIDLITDDSLEKIDKQLGMICASIPTYKTTYGKVSLEELDLTVKDREISSHHHHHH
                     GIKSMTYTFTGPIDRQLFYQFIMKLPESVLRLKGYVSFRNQPNAIYEFQYAYGLPDYG
                     IIGMQLPLTIVIIGETLDTNHIRNQLDMLQFT"
     gene            6825..7268
                     /gene="mgrA"
                     /locus_tag="NBI57_001455"
     CDS             6825..7268
                     /gene="mgrA"
                     /locus_tag="NBI57_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MgrA"
                     /protein_id="HCD1822399.1"
                     /translation="MSDQHNLKEQLCFSLYNAQRQVNRYYSNKVFKKYNLTYPQFLVL
                     TILWDESPVNVKKVVTELALDTGTVSPLLKRMEQVDLIKRERSEVDQREVFIHLTDKS
                     ETIRPELSNASDKVASASSLSQDEVKELNRLLGKVIHAFDETKEK"
     gene            complement(7395..9068)
                     /locus_tag="NBI57_001456"
     CDS             complement(7395..9068)
                     /locus_tag="NBI57_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding/permease
                     protein"
                     /protein_id="HCD1822400.1"
                     /translation="MKTRLKFQVDKDLLLAIVVGVCGSLVALAMFFLSGYMVTQSALG
                     APLYALMILVVTVKLFGFLRAITRYVERLISHKATFTMLRDIRVQFFGKLVNVIPNVY
                     RKLSSSDLISRMISRVEALQNIYLRVYYPPVVIGLTALVTVIVLAFISIGHALLIMVS
                     MLFTLLIVPWLSSKKARTLKKHAANEQARFLNHFYDYKAGMDELRRFNQINHYRDNLM
                     AKLNHFDKLQLKEQRFLTIYDFILNIIAMLSIFGSLVLGLIQINAGQLNIIYMTSIVL
                     MILTLFEQAVPMTNVAYYKADTDQALHDINEVISVPSTNGKKRLNDKYDATNIYEVKD
                     ASFKYWNQQTYVLSDINFNVNRGEKIAIVGPSGSGKSTLLQIMAGLYQLDSGSVRFEN
                     MDMFEIDDKDKFESLNVLLQSQQLFDGTIRQNLFTDEKDEAVQAIFKQLDLEHLALER
                     QIDLDGHTLSGGEIQRLAITRMLLKDTASTWILDEPTTALDKQNSLKVMDLIEAHAET
                     LIVATHDLTLLSRFETIIVMINGKIVEKGNYQQLLANQGALWNMIQYNA"
     gene            complement(9065..10696)
                     /locus_tag="NBI57_001457"
     CDS             complement(9065..10696)
                     /locus_tag="NBI57_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HCD1822401.1"
                     /translation="MKKLTTILFQYKIFPVLMFLVSTGLGILVITQNILIADFLAKII
                     RHQFQGLWIVLFILLGVLLLRATVQFLNQWLGDTLAFKVKHMLRQRVIYKNNGHPIGE
                     QMTILTENIDGLAPFYKSYLPQVFKSMMVPLIIIIAMFFIHFNTALIMLITAPFIPLF
                     YIIFGLKTRDESKDQMTYLNQFSQRFLNIAKGLVTLKLFNRTEQTEKHIYDDSTQFRT
                     LTMRILRSAFLSGLMLEFISMLGIGLVALEATLSLVVFHNIDFKTAAIAIILAPEFYN
                     VIKDLGQAFHTGKQSEGASDVVFEFLEQPNYNNEFLLKYEENQKPFIQLTDISFRYDD
                     SDRLVLNDLNLEIFKGDQIALVGPSGAGKSTLTHLIAGVYQPTIGTISTNQRDLNIGI
                     LSQQPYIFSASIKENITMFKDIENNTIEEVLDEVGLLDKVQSFTKGINTIIGEGGEML
                     SGGQMRRIELCRLLVMKPDLVIFDEPATGLDIQTEHMIQNVLFQHFKDTTMIVIAHRD
                     NTIRHLQRRLYIENGRLIADDRNISVNITENGDDL"
BASE COUNT         3659 a         1915 c         1507 g         3662 t
ORIGIN      
        1 gatacatcgc tagaatttac tttttaattt tatttatcgt ctcccttttt gagactaaaa
       61 tttttcaatt tatgacttta aatttgtttt tagcatatat accttttgag cttagtctat
      121 tattacgatt attcaaaccg aataaaaagt atgaatggcc actctttatc atatatagtt
      181 ttatatttgt gttactttta ccaaatacat tttatatggt aactgattta atccatttga
      241 accaatttca attcaacttt tacgcaggac taaatttaag tgagtggaaa tactttacct
      301 atttactgct tggtgtcttc ttagcggttt atgtgatgat tttaatctat tttgaaatac
      361 taacattgac ttctcaccgt tggcttaata gattaattgt tgtcgtgcta atgtttttaa
      421 atggattcgg aatttatatt ggtcgtttct tacgcttaca ttccgtttat ttctttaatg
      481 agcccttaca tgttctaaat cacattctag aaagtctaac gcttaaaact gctatgtttg
      541 tatgttttat ggttgtcatg caagcagcaa ttctattatt tggaaaagga gtgcgtttga
      601 acaagtgaca ataaatataa tcgttttaat agtatctatc attgtctttc aattgattat
      661 tggacacata tggcatgata ttggtttaag ttatttaaga agtatcttac tcatgatgct
      721 gccatttgga ctaggtgtat ttatacaaca agtgtcgtat tacgaacgac aatatcctaa
      781 atggcaagtg cctcaaaaca taaaagttag attgaaatat atttatctcg ctacgttttt
      841 agagtatgtc gtactatacc tcactttatt tacggacata ctaagataac atcaatttaa
      901 taagaaaact aaatacaact tatatatttg aggaaagcca atttctatca catcaataat
      961 agaaattggc ttatttatat tgcttaagaa ctttatattc tgctccagat tttactcatg
     1021 ccagatctat caattgagcc gaattagtct agcgctttaa atgtagctaa aacttgtgta
     1081 cgtttttctt gatgatctac catttgtctt ggataatgac tacctaattc aatcccctgt
     1141 tcaaaaaggt tggactcatt gcgttgtgta tcatgcaaat attttgcatc aatttgatta
     1201 aaaatcggaa gatatgtttt gatatacaaa gctttagcat caaagcgttc actctgtctt
     1261 attggattaa acattctaaa atacggcact gcatccgtac ctgtagaagc agaccattgc
     1321 catccatgaa tatttgatgc tgcatcatag tcaataaggt gctttctaaa gaatttttct
     1381 ccccatgtcc aatctataaa taaatctttg gttaaaaatt gcgacacaac cattctcatt
     1441 cgattatgca taaaaccagt ttgtgtcaat tccattattg ctgcatcaat gattggaaat
     1501 cctgtttgcc cttcgcacca tgcattaaaa ttcgcttcat tttgcgacca ttttatctgt
     1561 cgatatttag gtttgaaagc ttggtatgag gtttctggat actgtgtcat taacacataa
     1621 taaaattctc taaaaatgag ttcacgtata aatgcttcat ggtttgcctc atcttcatca
     1681 taacgcgcca ataaatcatt aaaaatttca ataatatcta ataatccata tgctaaagca
     1741 acacttagct gacttgttaa cacttcaggt aaatagtctc ttccgctttc gtaattctgt
     1801 atatcttgat ctaaaaaatg ttgccattta ttctgttcaa ttacttgtga ggatccaaac
     1861 gctatgtcat caaacactaa atcatcaggt gatgcaatga caaagtcttc gaatgatttt
     1921 aaatcatagt gatatacgtc tctatgcctc aagtaaggtc gccattttct atagaaacta
     1981 gtaaaaactt tgtatggctc cccttgttga ttcattgttt tactcgcttt aaagtaatga
     2041 ttcccacgaa ttaaagtaac ggcaatacgt gcttcattga ataaagaacg ttgatgtaaa
     2101 atatcatatt cttcaagatg ataactcata atatcacccg caatcactac ttcagagata
     2161 ttgttctctt tacaaaatga aatgatatcg tcataagaca taacataagg tgtaagcttt
     2221 tctgcgtgca acgattggta aaagcgctgc aaagtaccgc gataaaactg tgctttcaaa
     2281 tcagaagctg aagacatgtc ctcttccgga atgataaaat aacatgcatc aatgtcttct
     2341 ttttgttgat aaatatattc aaataatgga ttgtgttcca ttcgaaacat tcgatttaat
     2401 aacacagcaa ttgccataaa caaaacaccc ttcaattatc tttttatttt attaagcttt
     2461 ttgtcacttt aaaataacct agattcttat ttatattttt aattaaagca aacctaacca
     2521 tttccaataa gtcatactaa aaactatcac tagaatatac cctacaattg tcaatggtat
     2581 acctgccttc aagaaatctt taaccgtaaa agtaccagtg ccataagcca acatattttg
     2641 aggtgcacta acaggtaata agaaaccaaa actaataaca aattgttgaa ttaaaacaaa
     2701 tcctatagac tggtctccta agtgtaacgt agaggttagc gaaataaaaa caggtattaa
     2761 cgctgatgat aaacttgttg cactcgcaaa gcccaaatga atcaatatat taaaaagcgt
     2821 gataagtgca attgtcgcga taataggtaa atgttttaaa cctaaaacac caaaagtttg
     2881 atcacttaac cattgagctg cacctgtttt caaaagaacg ttccctagtg aaatacctac
     2941 accaaacacg ataattgttc cccatggtat tttattttca acatgtttcc atgtcatgac
     3001 accaattttc ggcattaaca taacacctaa agcaataata gtaatggatg cagagtcaat
     3061 cggatgtaat actttttcag ttgaccaaaa cagtaataat aacatcgata taacaattaa
     3121 acgccattca cgtgggctaa cggggccaag tttatgcaat tcttctttta ttaaatcttt
     3181 accaccttct attgtattaa tttctggagg catcacttta atcatgatga aatataaagc
     3241 tacggacata actatggacc aaggcgctgc atataagaac cactcgcccc atgaaacatc
     3301 aaatcctaat tgatggttta taaaattaat cgctacgata ttttgtgctg ccgccgtttt
     3361 gataccaata ttccaaattg acacagcttg tactgaagta attattaata aagacgctaa
     3421 cttgctatct ttggaaactt taaatgccgc aatcataccc agcaagattg gtacaactgc
     3481 ccctgctcta gctgttgcag aaggaacgaa aaatgcaagt acaattgaaa cgataattgc
     3541 tccaataact atatttctag ttttattacc aacaattgat aacactaaaa gagctagtct
     3601 tttatgcaaa ttcgtttctt gcatagcagc agccaaaaat aatgcagctg caacgagagc
     3661 tacagctgaa gttgcatatc cactaaacgc taatgataat gcatgattag ttcctagaag
     3721 gtcacttcca gctaaaatag cactgccact tttcggatta cctagcttct cccctaaatt
     3781 ttgaaccggg ctaaatccta aaagtaatat cattaagcca ataattaaag ttgctgacac
     3841 cggatatgat acagcttccg ttacccacat aataactgca aaagctaaaa tagctagtac
     3901 tgccttagcc attatcggca gacttgaagg tgccgggagt aatagtacaa ctataagtat
     3961 tataaaactc atgacaatcc aaaatggctt gtattgcttt tttgtctttt ctttcttttg
     4021 ttgttcatgt tccactacat gtttttcttc tgacataaca gtcacccctt tgtttaatct
     4081 taatttaatt ataacaattt tttaaaacat acatttttaa tttatgcaaa atacacaatt
     4141 ttatcgaaat attttgtaaa taatattgct aggtgatgag ttatctcggt taattacaaa
     4201 acgatatcta agtttaaata ttagatcata aaaaaactat ctactgaata tcctcatagc
     4261 atcatttttc taagcaaaag ttaaacttag ttagataaaa tgcaaatgag attattgtag
     4321 atagtctctt tttaatattt aaaaatgatt aatgccataa acataaataa ttccaaagaa
     4381 tgccgcgcca ctaagcactg ttaatataag acttagtaac aatgtgcgtt tataatgttt
     4441 aataaatgct atgaacaaaa tgactacatt atacgcaaag aaaagtccga ataatgtcat
     4501 tgttattttc aaatcagaat agaaaatatt taatagtaat acaatgactg cataaattaa
     4561 atacggaata actataaatt tgcgttcaaa tcttaattgt tcgaaacgtt tatcttcgtt
     4621 tgtcatgata tgctccttta tgatttaaaa gtaaataagt ttacggaata tcttgtccga
     4681 taatagcggt gtaaatgtca aaccacgact gatcatctaa attaagttgt agcccttcga
     4741 ttgcttgatc aatacgcttt aactgacttg ttccaagtat cggcatgata ctatgcggta
     4801 tttttactaa ccatgctatc atcacagctg tgtcactcac accatatttg tcagctattg
     4861 attgaacaac tttcataata cgttgcgctt taatatcttc cttgtcgaaa attttaccgc
     4921 ctgcaaaagg actccaagcc ataatttgaa catggttttg atacattgaa tccattgttc
     4981 catcttgtaa actatcaacg tgatatggcg ataattctaa ttgattgatg ctaatatgta
     5041 gtctttcttt cataatatat tgatttaaca attggtattg tgaatgatta aaattcgaca
     5101 ccccgaatga cttcaactta ccttgtttaa caagtttagt taatgcatca gcaacttgtt
     5161 ctggatccat caatggtgaa ggacgatgaa tgagtagact atctaaataa tctacattca
     5221 aattgattaa tgactgttca acagatttca cgatgtgctt actactcaaa tcataacgat
     5281 gtccatttgt aaaatcaaat tgcttagaag gcaaaatgat accacatttc gtaacaattt
     5341 gaattttatt tcttaattcg ggtgataaat ccaaagcatt accaaacagt gattcacatt
     5401 gataatcccc ataaatatca gcatgatcca tcgttgtaat tccacgttca actaattcat
     5461 ttataaaata atttaactct ttcgcagtca tcttccattc atttgcacgc caaaaacctt
     5521 gtacaagcct agaaaaatga acatagtgat taatcattat ttgttccata tctcatccac
     5581 tccatccctc aatgtcacaa aatattcaca ataaaagtat taagaaatta atgtgctatt
     5641 tccgttaaat tgaaacaaca cattttctga tttacgtaaa ttgtagcata tccaattgat
     5701 tacgtatgtg atttgtatct aaagtttcac caataataac aatcgttaat ggtaattgca
     5761 tgccaattat tccatagtct ggtaaaccat atgcatattg aaattcataa attgcatttg
     5821 gttgatttct aaatgacaca taacctttca aacgtagaac agattccggt aatttcatta
     5881 taaattgata aaacaattga cgatcaatcg gacctgtaaa cgtgtaagtc atacttttaa
     5941 tcccatgatg atggtgatga tgagacgata tctctctgtc tttaacagtt aagtccaatt
     6001 cttccaacga aacttttcca taggttgttt tataagttgg aatacttgca caaatcatac
     6061 ctaattgctt atcaattttc tcaagactgt catcagttat aagatcaatt ttattaataa
     6121 taatcatgtc acttagtttc aactgatctt ccatcagcga aactgtattt tcggtatatt
     6181 gatgacgttc taaaaatcga gtcgcatcta atacaccata aataatcggc ttttcaaaga
     6241 aattaacgat ttgcggatct tgacatgcaa ctagtaattc caaaggatgc gcaatgcctg
     6301 tcgcttctat gatgacatga ttaacgtccc cttttaaagc aatggctttt agttcataga
     6361 caagttcttg tttaagatcg caacaaacac aaccattaat caatgaatgg acttcaattt
     6421 catttgaaat actattgcta tcaatatcaa aagtaccgaa ttcattcatg atgattttaa
     6481 ttttctcatc attttttaat aattcactaa tataatgtgt cagtaacgtg gttttaccac
     6541 tacccaaaaa tccgttaatt atggatattc ttattttttc atctttatta tttttcattt
     6601 tatattctct tttctattac agttattttt aaaaatgata acatatatat taaattatct
     6661 ttttgcttga tttaatcaaa aatatcttct aaactttaaa catactttaa ttttagcatg
     6721 gcattgaatc aaagaaagtt gtgaattaat aaacaatcaa ctttttaatg attaccaatc
     6781 cgaaaagaga aaaaaacgga tagtatgtct ggaggagaac tttaatgtct gatcaacata
     6841 atttaaaaga acagctatgc tttagtttgt acaatgctca aagacaagtt aatcgctact
     6901 actctaacaa agtttttaag aagtacaatc taacataccc acaatttctt gtcttaacaa
     6961 ttttatggga tgaatctcct gtaaacgtca agaaagtcgt aactgaatta gcactcgata
     7021 ctggtacagt atcaccatta ttaaaacgaa tggaacaagt agacttaatt aagcgtgaac
     7081 gttccgaagt cgatcaacgt gaagtattta ttcacttgac tgacaaaagt gaaactatta
     7141 gaccagaatt aagtaatgca tctgacaaag tcgcttcagc ttcttcttta tctcaagatg
     7201 aagttaaaga acttaatcgc ttattaggta aagtcattca tgcatttgat gaaacaaagg
     7261 aaaaataatt aacttttgtc atgacaatta aagtaatgtt tagaatttat taagaataga
     7321 aaaacaatta gcacgcgtaa gcttgttagt taaaaaactg cttgaaaggt ttcttagcct
     7381 atcaagcagt ttttttatgc attatattga atcatattcc ataaagcacc ttgattagcg
     7441 agtaattgtt gatagtttcc cttttcaact attttaccat ttatcatcac aatgatggtc
     7501 tcaaaacgtg acaataaagt taaatcgtgt gtagcaacaa ttaatgtttc tgcatgtgct
     7561 tcaattaaat ccataacttt taaactattt tgtttatcta atgcagttgt tggttcatct
     7621 aaaatccatg ttgatgcagt atcttttaat aacatcctcg taatcgctaa acgctgaatt
     7681 tctccgccag ataatgtatg accatctaag tcaatttgac gttctagtgc caaatgttct
     7741 aaatctaatt gcttaaatat tgcttgcacc gcttcatctt tttcatcggt aaataaattt
     7801 tgacgtattg taccatcaaa taattgttga gattgtagca agacatttaa cgattcaaac
     7861 ttatctttgt catctatttc aaacatatcc atattttcga aacgaacaga gccactatct
     7921 aattgatata accctgccat aatttgtagt aatgtacttt ttcctgaacc tgaaggaccc
     7981 acaatcgcaa tcttttcgcc tctattaaca ttaaaattaa tatccgacaa cacatacgtt
     8041 tgctgattcc aatacttaaa actagcatcc ttaacttcat aaatgttcgt tgcatcatac
     8101 ttatcattaa gacgtttttt tccattagta gaaggtacag atatcacttc attaatatcg
     8161 tgcaatgctt ggtcagtatc cgctttataa tacgcgacat ttgtcattgg tacagcttgt
     8221 tcaaataaag ttaagatcat taaaactata ctcgtcatat aaataatatt tagttggcct
     8281 gcattaattt gaattaatcc tagaactaaa ctaccaaaaa tcgaaagcat agcaataata
     8341 tttaatataa aatcataaat cgttaaaaag cgttgctctt taagttgtaa tttatcaaaa
     8401 tgatttaatt tagccatcaa attatctcga taatgattaa tttgattaaa tcgacgtagt
     8461 tcatccatac cagctttata atcataaaaa tgatttaaaa atcgggcctg ttcattagct
     8521 gcatgtttct ttaaagtacg tgcttttttt gagcttaacc aaggaacaat gagtaaagta
     8581 aacaacatgc taaccataat caatagcgca tggccgattg aaatgaacgc caaaactatg
     8641 actgtaacta gcgctgtcaa accgataacg actggtggat aataaacacg taaatatata
     8701 ttttgtaatg cctcaacacg actaatcata cgtgaaatta aatcactaga actcagttta
     8761 cggtaaacat taggaatgac atttactaat ttaccgaaaa actgtacccg aatatcacgt
     8821 agcattgtaa atgtagcttt atgagaaata aggcgctcta cgtatcgagt aatagctctt
     8881 aaaaacccaa acaattttac tgtaacgact aaaatcatca gagcgtatag tggcgcacca
     8941 agtgcacttt gtgtcaccat ataaccactt aagaaaaaca tggcgagcgc aactaaactt
     9001 ccacaaacac caacaactat agctaacaat aaatccttat ctacttgaaa ttttagtcgt
     9061 gttttcataa gtcatcacca ttttctgtta tatttactga aatattgcga tcatcagcaa
     9121 tcagtcttcc attttctata tacaagcgtc gttgtaaatg gcgaattgta ttatctctat
     9181 gtgcaatgac aatcatcgtt gtatctttaa aatgttgaaa cagaacgttc tgaatcatgt
     9241 gttctgtttg aatatctaaa ccagttgcag gctcatcaaa tataacgaga tctggcttca
     9301 taactaaaag acggcaaagt tctatgcgtc tcatctgtcc accagataac atttcgcctc
     9361 cttcacctat tattgtgtta atgccttttg tgaaagattg cactttgtct aataaaccta
     9421 cttcgtctag cacttcttca atagtattat tttctatatc tttaaacatc gtaatattct
     9481 cttttataga agcactgaaa atatatggct gttgactaag tattcctata tttaaatcac
     9541 gctggtttgt acttatagta cctattgttg gctgataaac acctgcaata agatgtgtca
     9601 aagtggattt acctgccccg cttggaccta caagtgcaat ttgatcacct ttaaatattt
     9661 ccaaatttaa atcatttaat accaatctat cagaatcatc atatcgaaat gatatgtctg
     9721 ttaactgaat aaatggcttt tggttttcct catactttaa tagaaattca ttattatagt
     9781 tcggttgttc taaaaactca aacacaacgt cactggcacc ttcactttgt tttccagtat
     9841 ggaacgcttg ccctaagtcc ttaattacat tataaaattc aggcgctaaa ataatcgcaa
     9901 ttgccgcagt tttaaaatca atattatgaa atactactaa gcttagcgtt gcttccaatg
     9961 caaccaatcc aatacctaac atacttataa attcgagcat taatcccgat aaaaaagcac
    10021 tgcgtaaaat gcgcattgtt aaagttctaa actgagtact atcgtcgtaa atatgcttct
    10081 ctgtttgctc tgtacgatta aatagcttta acgtcactaa acctttagca atatttaaaa
    10141 accgttgact aaattgattc aaataagtca tttgatcttt tgactcatct cgcgttttca
    10201 aaccgaaaat aatataaaac aaaggaataa atggtgcagt tattaacata attaatgcgg
    10261 tattgaaatg gatgaaaaac attgcaatga ttatgatgag cggaaccatc attgatttga
    10321 acacttgagg caaataactc ttataaaaag gtgctaaacc atcaatgttt tctgtgagta
    10381 tagtcatttg ttcaccgatt ggatgaccat tatttttata aataacccgc tgtctaagca
    10441 tatgcttaac tttaaatgct aatgtatcac ctaaccattg atttagaaat tgcacagttg
    10501 ctcttaaaag taaaacacct aataaaataa ataatacaat ccataaacct tgaaattgat
    10561 gtcttataat tttagctaaa aaatctgcta ttaaaatatt ttgcgttata acgagtatgc
    10621 cgagaccagt actgaccaag aacatgagta ccggaaaaat tttatattga aacagtattg
    10681 ttgttaattt tttcacaatt atatcaccta aacctattat aaaaggtttt ccttaaaatc
    10741 aaa
//
LOCUS       DAHHSC010000075        10734 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.075, whole genome shotgun sequence.
ACCESSION   DAHHSC010000075 DAHHSC010000000
VERSION     DAHHSC010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 10734)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10734)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10734
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.075"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            182..1666
                     /locus_tag="NBI57_001458"
     CDS             182..1666
                     /locus_tag="NBI57_001458"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000340119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HCD1822402.1"
                     /translation="MDASTLFKKVKVKRVLGSLEQQIDDITTDSRTAREGSIFVASVG
                     YTVDSHKFCQNVADQGCKLVVVNKEQSLPANVTQVVVPDTLRVASILAHTLYDYPSHQ
                     LVTFGVTGTNGKTSIATMIHLIQRKLQKNSAYLGTNGFQINETKTKGANTTPETVSLT
                     KKIKEAVDAGAESMTLEVSSHGLVLGRLRGVEFDVAIFSNLTQDHLDFHGTMEAYGHA
                     KSLLFSQLGEDLSKEKYVVLNNDDSFSEYLRTVTPYEVFSYGIDEEAQFMAKNIQESL
                     QGVSFDFVTPFGTYPVKSPYVGKFNISNIMAAMIAVWSKGTSLETIIKAVENLEPVEG
                     RLEVLDPSLPIDLIIDYAHTADGMNKLIDAVQPFVKQKLIFLVGMAGERDLTKTPEMG
                     RVACRADYVIFTPDNPANDDPKMLTAELAKGATHQNYIEFDDRAEGIKHAIDIAEPGD
                     TVVLASKGREPYQIMPGHIKVPHRDDLIGLEAAYKKFGGGPVDQ"
     gene            1647..1907
                     /locus_tag="NBI57_001459"
     CDS             1647..1907
                     /locus_tag="NBI57_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueH family protein"
                     /protein_id="HCD1822403.1"
                     /translation="MALLINKRFIDEGKTIDVYLFEALNNQIIIAIPDWFWSYQMAMT
                     LDEETCFEAILMQLFVFKEEEEAESIASQLTDWIETYKKEKD"
     gene            1907..3469
                     /locus_tag="NBI57_001460"
     CDS             1907..3469
                     /locus_tag="NBI57_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HCD1822404.1"
                     /translation="MNLKQEVESRKTFAIISHPDAGKTTLTEKLLYFSGAIREAGTVK
                     GKKTGKFATSDWMKVEQERGISVTSSVMQFDYDDYKINILDTPGHEDFSEDTYRTLMA
                     VDSAVMVIDCAKGIEPQTLKLFKVCKMRGIPIFTFINKLDRVGKEPFELLDEIEETLN
                     IETYPMNWPIGMGQSFFGIIDRKSKTIEPFRDEENILHLNDDFELEEDHAITNDSAFE
                     QAIEELMLVEEAGEAFDNDALLSGDLTPVFFGSALANFGVQNFLNAYVDFAPMPNARQ
                     TKEDVEVSPFDDSFSGFIFKIQANMDPKHRDRIAFMRVVSGAFERGMDVTLQRTNKKQ
                     KITRSTSFMADDKETVNHAVAGDIIGLYDTGNYQIGDTLVGGKQTYSFQDLPQFTPEI
                     FMKVSAKNVMKQKHFHKGIEQLVQEGAIQYYKTLHTNQIILGAVGQLQFEVFEHRMKN
                     EYNVDVVMEPVGRKIARWIENEDQITDKMNTSRSILVKDRYDDLVFLFENEFATRWFE
                     EKFLEIKLYSLL"
     gene            3771..4574
                     /locus_tag="NBI57_001461"
     CDS             3771..4574
                     /locus_tag="NBI57_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HCD1822405.1"
                     /translation="MLMDPSLILPYLWVLVVLVFLEGLLAADNAIVMAVMVKHLPPEQ
                     RKKALFYGLLGAFVFRFLALFLISIIANFWFIQAAGAVYLIYMSIKNLWQFFKHPEIE
                     SPEAGDDHHYDESGEEIKASNKSFWGTVLKIEFADIAFAIDSMLAALAIAVTLPKVGI
                     HFGGMDLGQFVVMFLGGMIGVILMRYAATWFVELLNKYPGLEGAAFAIVGWVGVKLVV
                     MVLAHPDIAVLPEHFPHGVLWQSIFWTVLIGLVIIGWLGSVVKNKKSHK"
     gene            4793..7117
                     /locus_tag="NBI57_001462"
     CDS             4793..7117
                     /locus_tag="NBI57_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HCD1822406.1"
                     /translation="MEWTLVDIGKKHVIPKSQYRRKRREFFHNEDREENLNQHQDKQN
                     IDNTTSKKADKQIHKDSIDKHERFKNSLSSHLEQRNRDVNENKAEESKSNQDSKSAYN
                     RDHYLTDDVSKKQNSLDSVDQDTEKSKYYEQNSEATLSTKSTDKVESTEMRKLSSDKN
                     KVGHEEQHVLSKPSEHDKETRIDSESSRTDSDSSMQTEKIKKDSSDGNKSSNLKSEVI
                     SDKSNTVPKLSESDDEVNNQKPLTLPEEQKLKRQQSQNEQTKTYTYGDSEQNDKSNHE
                     NDLSHHTPSISDDKDNVMRENHIVDDNPDNDINTLSLSKIDDDRKLDEKIHVEDKHKQ
                     NADSSETVGYQSQSTASHRSTEKRNISINDHDKLNGQKTNTKTSANNNQKKATSKLNK
                     GRATNNNYSDILKKFWMMYWPKLVILMGIIILIVILNAIFNNVNKNDRMNDNNDADAQ
                     KYTTTMKNANNTVKSVVTVENETSKDSSLPKDKASQDEVGSGVVYKKSGDTLYIVTNA
                     HVVGDKENQKITFSNNKSVVGKVLGKDKWSDLAVVKATSSDSSVKEIAIGDSNNLVLG
                     EPILVVGNPLGVDFKGTVTEGIISGLNRNVPIDFDKDNKYDMLMKAFQIDASVNPGNS
                     GGAVVNREGKLIGVVAAKISMPNVENMSFAIPVNEVQKIVKDLETKGKIDYPDVGVKM
                     KNIASLNSFERQAVKLPGKVKNGVVVDQVDNNGLADQSGLKKGDVITELDGKLLEDDL
                     RFRQIIFSHKDDLKSITAKIYRDGKEKEINIKLK"
     gene            7134..8492
                     /locus_tag="NBI57_001463"
     CDS             7134..8492
                     /locus_tag="NBI57_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HCD1822407.1"
                     /translation="MSIFSQFLKRSSPQQGIVLYYIVAIVIAFLLLNLPYVHKPGVEV
                     NPIDTLFVAVSGISVTGLSPISIVDTYSTFGQLIILVILNIGGIGVMAIGTMLWVVLG
                     KHIGIRERQLIMLDNNKNTMSGTVKLIIDIVKSIFVIELVGAMLLAFYFYRDNPDLKY
                     AIMQGVFVSISATTNGGLDITGKSLIPYAHDYFVQAIVIFLIILGSIGFPVLLEVKAY
                     IQNRVTNFRFSLFTKITTSTYLFLFIVGVLAILLFEHNHAFKGLSWHQSLFYSLFQSA
                     TTRSAGLQTIDVTTLSDPTNIIMGILMFIGSSPSSVGGGIRTTTFAILILFLINFSNN
                     ADKTSIKVYNREVHIMDIQRSFAVFTMATILTFLGMLIISATENGKLTFLQVFFEVMS
                     AFGTCGLSLGVTSDISDISKVVLMVLMFIGRVGLISFIIMIAGRREPDKFHYPKERIQ
                     IG"
     gene            8631..10142
                     /locus_tag="NBI57_001464"
     CDS             8631..10142
                     /locus_tag="NBI57_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HCD1822408.1"
                     /translation="MEKNENINVEILTTSDMHSHFLNGDYGSNIYRAGTYVNQIRAQN
                     HRVILLDSGGSLAGSLAAYYYAIVAPYKRHPMIKLMNRMHYDASGVSPSEFKFGLSFL
                     TRSIALARFPWLSANIEYNVTKEPYFSTPYCIKHFGDLKIAIVGVTADGLMENEYSEM
                     EQDVSIEKTLVASKRWIRYIHEVEEPDFLIVIYHGGLNKISNSTKNKKASSNEAEKLM
                     EELGVIDLMITAHQHQTIVGQDHETYYVQAGQDAKELVHLSINFKKRTTTYDVESIDS
                     KVIDLNEYEEDQELLDLTFYDRKAVAYWSQEIISDKGLMLSVNGLQDLVCQTHPFSQL
                     LHDAIQLAFDNDITCVHVPMNGEKGLSGQIRNEDLYHAYPYPDKPMDMTISGQNIKDI
                     LEYSYSHLDFVNEQLSLTIIDETLCTMWQGFNYEIDMNQEPGQRVMLDQIDLTKSYRV
                     TMTDYCYRNYKNYLKNAIIHESYDETMSTLIAEKLRDPNYRISCSDNFVVKNR"
     gene            complement(10322..10410)
                     /locus_tag="NBI57_001465"
     tRNA            complement(10322..10410)
                     /locus_tag="NBI57_001465"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10373..10375),aa:Ser,seq:gct)
     gene            complement(10413..10487)
                     /locus_tag="NBI57_001466"
     tRNA            complement(10413..10487)
                     /locus_tag="NBI57_001466"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10453..10455),aa:Asn,seq:gtt)
     gene            complement(10629..>10734)
                     /locus_tag="NBI57_001467"
     CDS             complement(10629..>10734)
                     /locus_tag="NBI57_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001619687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="competence protein ComK"
                     /protein_id="HCD1822409.1"
                     /translation="SRMISKNPDQPIDYNKATLNVFEALTRYSLFEDK"
BASE COUNT         3848 a         1451 c         2069 g         3366 t
ORIGIN      
        1 tggttttaat aatttaagat gagccataat tgtaaaacta attcataata ccgtatgttt
       61 tatttttaat agtagaaatt agaaaatgct gattagtagg atataacagt gaaattataa
      121 atttattaac atcaacaaaa cgtgtataat aaacatattg tagaaaaagg agcggttcag
      181 tttggatgca agtacgttgt ttaagaaagt aaaagtaaag cgtgtattgg gttctttaga
      241 acaacaaata gatgatatca ctactgattc acgtacagcg agagaaggta gcatttttgt
      301 cgcttcagtt ggatatactg tagacagtca taagttctgt caaaatgtag ctgatcaagg
      361 gtgtaagttg gtagtggtca ataaagaaca atcattacca gctaacgtaa cacaagtggt
      421 tgtgccggac acattaagag tagctagtat tctagcacac acattatatg attatccgag
      481 tcatcagtta gtgacatttg gtgtaacggg tacaaatggt aaaacttcta ttgcgacgat
      541 gattcattta attcaaagaa agttacaaaa aaatagtgca tatttaggaa ctaatggttt
      601 ccaaattaat gaaacaaaga caaaaggtgc aaatacgaca ccagaaacag tttctttaac
      661 taagaaaatt aaagaagcag ttgatgcagg cgctgaatct atgacattag aagtatcaag
      721 ccatggctta gtattaggac gactgcgagg cgttgaattt gacgttgcaa tattttcaaa
      781 tttaacacaa gaccatttag attttcatgg cacaatggaa gcatacggac acgcgaagtc
      841 tttattgttt agtcaattag gtgaagattt gtcgaaagaa aagtatgtcg tgttaaacaa
      901 tgacgattca ttttctgagt atttaagaac agtgacgcct tatgaagtat ttagttatgg
      961 aattgatgag gaagcccaat ttatggctaa aaatattcaa gaatctttac aaggtgtcag
     1021 ctttgatttt gtaacgcctt ttggaactta cccagtaaaa tcgccttatg ttggtaagtt
     1081 taatatttct aatattatgg cggcaatgat tgcggtgtgg agtaaaggta catctttaga
     1141 aacgattatt aaagctgttg aaaatttaga acctgttgaa gggcgattag aagttttaga
     1201 tccttcgtta cctattgatt taattatcga ttatgcacat acagctgatg gtatgaacaa
     1261 attaatcgat gcagtacagc cttttgtaaa gcaaaagttg atatttttag ttggtatggc
     1321 aggcgaacgt gatttaacta aaacgcctga aatggggcga gttgcctgtc gtgcagatta
     1381 tgtcattttc acaccggata atccggcaaa tgatgacccg aaaatgttaa cggcagaatt
     1441 agccaaaggt gcaacacatc aaaactatat tgaatttgat gatcgtgcag aagggataaa
     1501 acatgcaatt gacatagctg agcctgggga tactgtcgtt ttagcatcaa aaggaagaga
     1561 accatatcaa atcatgccag ggcatattaa ggtgccacat cgagatgatt taattggcct
     1621 tgaagcagct tacaaaaagt tcggtggtgg ccctgttgat caataaaaga tttattgatg
     1681 aaggtaaaac tattgatgtt tatttattcg aagcattaaa taaccagata atcattgcta
     1741 taccagattg gttttggtca tatcagatgg caatgacatt agatgaagaa acttgttttg
     1801 aagcaatact catgcaattg tttgttttta aagaagagga agaggcagaa tcgattgcat
     1861 cacaactaac agattggata gaaacatata aaaaggagaa agactaatga acttaaagca
     1921 agaagttgag tctagaaaga cttttgcgat tatttcacat cccgatgcag ggaaaacaac
     1981 gttaactgaa aaactattgt acttcagtgg tgctattcgt gaagcgggta cagttaaagg
     2041 gaagaagact ggtaaatttg cgacaagtga ctggatgaaa gttgaacaag agcgtggtat
     2101 ttctgtaact agttcagtaa tgcaatttga ttacgatgat tataaaatca atatcttaga
     2161 tacaccagga catgaagact tttcagaaga tacgtataga acattaatgg cagttgacag
     2221 tgctgtcatg gtcatagact gtgcaaaagg tattgaacca caaacattga agttatttaa
     2281 agtttgtaaa atgcgtggta ttccaatctt tacattcatt aataaattag accgagtagg
     2341 taaagaacca tttgaattat tagatgaaat cgaagagaca ttaaatattg aaacataccc
     2401 tatgaattgg ccaattggta tgggacaaag tttctttggc atcattgata gaaagtctaa
     2461 aacaattgaa ccatttagag atgaagaaaa tatattacat ttgaatgatg attttgagtt
     2521 ggaagaagat catgcaatta caaatgatag tgcttttgaa caagcgattg aagaattaat
     2581 gttggttgaa gaagcgggtg aagcctttga taatgacgcg ctgttgagtg gagacttaac
     2641 acctgtattt ttcggttcag ctttagctaa ctttggtgta caaaatttct taaatgcata
     2701 tgttgatttt gcgccaatgc caaatgcgag acaaacaaaa gaagacgttg aagtaagccc
     2761 gtttgatgat tcattttcag gatttatctt taaaattcaa gccaacatgg accctaaaca
     2821 ccgtgataga attgccttta tgcgtgtcgt tagtggtgca tttgaacgtg gtatggatgt
     2881 tactttgcaa cgtactaata aaaagcaaaa gatcacacgt tcaacgtcat ttatggcaga
     2941 cgataaagaa actgtgaatc atgctgtagc aggcgatatc attggactat atgatactgg
     3001 taattatcaa attggagata ctttagttgg tggaaaacaa acctacagtt tccaagattt
     3061 accacaattt acgccagaaa tttttatgaa agtttctgct aaaaacgtca tgaaacagaa
     3121 gcatttccat aaaggtattg aacaattagt acaagaaggt gcgattcaat actataaaac
     3181 attacacaca aaccaaatta ttttaggtgc tgttggtcag ttacaatttg aagttttcga
     3241 acatagaatg aaaaacgaat ataatgttga tgttgttatg gagccagtag gccgtaaaat
     3301 tgcacgttgg attgaaaatg aagaccaaat tacagataag atgaacacat caagatcgat
     3361 tttagtgaaa gatagatatg acgatttagt attcttattt gaaaatgaat ttgcaacaag
     3421 atggtttgaa gagaaattcc ttgaaattaa attgtatagt ttactttaac agctcaattg
     3481 tataatcgaa tttgttacat taaaaataat tgtttcgttg aagaaaaata aattgtatat
     3541 tttaaaagaa aaaggtatac tatgatgtat caaatgaata acctatggca ttttgtcaga
     3601 ggggagtaac ttaagaatca tgaccgtata aatgattcga cactttatcg tcattacgaa
     3661 gatatcttcc ggtaaagtgg gcaatttaaa ttgcttagtg agacctttgc tatttattta
     3721 gcataggtct ttttgtttgt acttaactta tttatttaaa ggagttgtac atgttaatgg
     3781 atccaagttt gatcttacct tatttatggg tacttgtcgt tttagtattt ttagaaggct
     3841 tattagcagc agataacgcg attgttatgg ctgtaatggt taagcactta ccacccgaac
     3901 aacgtaaaaa agctttgttt tacggtttgt taggtgcatt tgtatttaga tttttagcat
     3961 tattcttaat tagtattatc gcgaactttt ggtttattca agctgcagga gcggtttact
     4021 taatttatat gtcaatcaaa aatctgtggc agttctttaa acacccagaa attgaaagtc
     4081 ctgaagctgg agatgatcat cattatgatg aatctggtga agagattaaa gcaagtaaca
     4141 aatcattctg gggaactgtg ttgaaaatag aatttgcaga tatcgcattt gccattgatt
     4201 ctatgcttgc tgctttagct attgctgtaa cacttcctaa agttggtatt cactttggtg
     4261 gtatggactt aggtcagttc gtagtcatgt tcctaggtgg aatgattggt gttattctaa
     4321 tgcgttatgc agcaacatgg tttgtagagc tattaaacaa atatccagga cttgaaggtg
     4381 cagccttcgc gatcgttggt tgggtaggtg ttaaattagt tgtcatggta ttagcgcacc
     4441 cagacatcgc tgtattgcct gagcacttcc cacatggcgt attatggcaa tctattttct
     4501 ggacagtact aattggatta gtaattatcg gttggttagg ttcagttgtt aaaaataaaa
     4561 aatcgcataa ataattgatg tgaagcggac aatcttaatt tagtttaagg ttgtcctttt
     4621 tcatttaatt gagtgattta tgaaaaatgg attttgaaga atgtgaatca aaagatgcga
     4681 tatagtatta agaaaatgtg ccttttatat ttagcatttt ttcaatagaa attatataga
     4741 ttttaaagca aattaggtgt taatgtgtca taatgataag tgattttatt gaatggagtg
     4801 gacattagtg gatattggta aaaaacatgt aattcctaaa agtcagtacc gacgtaagcg
     4861 tcgtgaattc ttccacaacg aagacagaga agaaaattta aatcaacatc aagataaaca
     4921 aaatatagat aatacaacat caaaaaaagc agataagcaa atacataaag attcaattga
     4981 taagcacgaa cgttttaaaa atagtttatc atcgcattta gaacagagaa accgtgatgt
     5041 taatgagaat aaagctgaag aaagtaaaag taatcaggat agtaagtcag catataacag
     5101 agatcattat ttaacagacg atgtatctaa aaaacaaaat tcattagatt cagtggacca
     5161 agatacagag aaatcaaaat attatgagca aaattctgaa gcgactttat caactaaatc
     5221 aaccgataaa gtagaatcaa ctgaaatgag aaagctaagt tcagataaaa acaaagttgg
     5281 tcatgaagag caacatgtac tttctaaacc ttcagaacat gataaagaga ctagaattga
     5341 ttctgagtct tcaagaactg attcagacag ctcgatgcag acagagaaaa taaaaaaaga
     5401 cagttcagat ggaaataaaa gtagtaatct gaaatctgaa gtaatatcag acaaatcaaa
     5461 tacagtacca aaattgtcgg aatctgatga tgaagtaaat aatcagaagc cattaacttt
     5521 accggaagaa cagaaattga aaagacagca aagtcaaaat gagcaaacaa aaacctatac
     5581 atatggtgat agcgaacaaa atgacaagtc taatcatgaa aatgatttaa gtcatcatac
     5641 accatcgata agtgatgata aagataacgt tatgagagaa aatcatattg ttgacgataa
     5701 tcctgataat gatatcaata cactatcatt atcaaaaata gatgacgatc gaaaacttga
     5761 tgaaaaaatt catgttgaag ataaacataa acaaaatgca gactcgtctg aaacggtggg
     5821 atatcaaagt cagtcaactg catctcatcg tagcactgaa aaaagaaata tttctattaa
     5881 tgaccatgat aaattaaacg gtcaaaaaac aaatacaaag acatcggcaa ataataatca
     5941 aaaaaaggct acatcaaaat tgaacaaagg gcgcgctacg aataataatt atagtgacat
     6001 tttgaaaaag ttttggatga tgtattggcc taaattagtt attctaatgg gtattattat
     6061 tctaattgtt attttgaatg ccatttttaa taatgtgaac aaaaatgatc gcatgaatga
     6121 taataatgat gcagatgctc aaaaatatac gacaacgatg aaaaatgcca ataacacagt
     6181 taaatcggtc gttacagttg aaaatgaaac atcaaaagat tcatcattac ctaaagataa
     6241 agcatctcaa gacgaagtgg gatcaggtgt tgtatataaa aaatctggag atacgttata
     6301 tattgttacg aatgcacacg ttgtcggtga taaagaaaat caaaaaataa ctttctcgaa
     6361 taataaaagt gttgttggga aagtgcttgg taaagataaa tggtcagatt tagctgttgt
     6421 taaagcaact tcttcagaca gttcagtgaa agagatagct attggagatt caaataattt
     6481 agtgttagga gagccaatat tagtcgtagg taatccactt ggtgtagact ttaaaggcac
     6541 tgtgacagaa ggtattattt caggtctgaa cagaaatgtt cctattgatt tcgataaaga
     6601 taataaatat gatatgttga tgaaagcttt ccaaattgat gcatcagtaa atccaggtaa
     6661 ctcgggtggt gctgtcgtca atagagaagg aaaattaata ggtgtagttg cagctaaaat
     6721 tagtatgcca aacgttgaaa atatgtcatt tgcaatacct gttaatgaag tacaaaagat
     6781 tgtaaaagat ttagaaacaa aaggtaaaat tgactatccc gatgtaggtg ttaaaatgaa
     6841 gaatattgcc agtctaaata gttttgaaag acaagcagtt aaattgccag gaaaagttaa
     6901 gaacggtgtt gttgtagatc aagttgacaa caatggttta gcagatcaat ctggtctgaa
     6961 aaaaggtgat gtaattactg aattagatgg caaactttta gaagatgatt tacgctttag
     7021 gcagattata tttagtcata aagatgactt gaaatcaatt acagcgaaga tttatagaga
     7081 tggtaaagag aaagaaatta atattaaact aaaataatgt tgaggtgaac aacgtgtcaa
     7141 tttttagcca gtttttaaaa agatcaagcc ctcaacaagg tattgtattg tactatatcg
     7201 tcgcaattgt cattgcattt ttattattaa acttaccgta tgttcataaa ccaggtgtag
     7261 aagtaaatcc aattgacaca ttatttgttg ccgtatccgg aattagtgtt actggattgt
     7321 ctccgataag tattgtcgat acctattcta catttggaca attaattatc ctcgtgatat
     7381 taaatattgg tggaattggc gtcatggcaa ttggtacgat gttatgggtg gtactaggta
     7441 aacatattgg aattagagaa cgtcagttaa ttatgttaga taataacaaa aacacaatga
     7501 gtggtaccgt caaattgatt attgatattg taaaatcaat atttgtaatc gaactcgtag
     7561 gagccatgtt attagcattt tacttttatc gagataatcc agatttaaaa tatgcaatca
     7621 tgcaaggtgt ttttgtttct atttctgcca ctaccaatgg tggattagat attacaggta
     7681 agtcattaat tccttatgca catgattatt ttgtacaagc gatagttata tttttaataa
     7741 ttttaggatc aatcggcttc ccagtattat tagaagttaa agcttatatt caaaataggg
     7801 ttactaattt tagattttca ttatttacta aaattacgac atcaacatat ttattcctat
     7861 ttattgttgg ggtattagcc attctattat ttgaacataa ccatgcgttc aaaggtttaa
     7921 gttggcatca atcgttattc tattcgctgt ttcaatcagc gactacaaga agtgcgggtc
     7981 ttcaaacaat tgatgtgaca acactaagtg accccactaa tattatcatg ggtattttaa
     8041 tgtttatagg atcttcgcca agttcggttg gtggcggtat tcgtacaaca actttcgcta
     8101 ttttaatttt gtttttaatt aactttagta ataatgccga taaaacatcc attaaagttt
     8161 acaatagaga agtacacatt atggatattc aacgttcatt tgcagtattt acaatggcga
     8221 caattttaac atttttagga atgctaatta tatcagctac tgaaaatggt aagcttacat
     8281 ttttacaagt attttttgaa gtcatgtctg catttggaac ttgtggacta tcgcttggtg
     8341 tcacaagtga tattagtgat atttctaagg tcgtactaat ggtactcatg tttataggac
     8401 gtgttggctt aatatcattt atcattatga tagcaggacg tcgagaacca gataaattcc
     8461 attatccaaa agaacgtatt caaataggat aatataatag caatctaagt ttagttaata
     8521 tagattttaa ctggaactta gattgctttt ttagtttgta tttttaactt attttataag
     8581 acgattggtt ttcaaaatgg taaactagta acaatgagag gtgtaacatg atggaaaaaa
     8641 atgaaaacat taatgtagag attttaacta cgtcagatat gcatagtcat ttcttaaatg
     8701 gtgattatgg ttcaaatatt tatagagctg gtacttatgt taaccaaata agagcacaaa
     8761 atcatcgcgt cattttatta gatagtggcg gaagtttagc tggctcgtta gcggcctatt
     8821 attatgctat tgttgcacct tataaacgac atccaatgat aaagttaatg aacagaatgc
     8881 attatgatgc tagcggtgtg agtccaagtg aattcaagtt tggtttatca tttttaactc
     8941 gttcaattgc tttggcacgt tttccatggt tatcagcaaa tattgaatac aatgttacta
     9001 aggagcctta tttttcaact ccatattgta ttaaacattt tggtgactta aaaattgcta
     9061 tcgtaggcgt cacagcagat ggtttaatgg aaaatgagta ttctgaaatg gagcaagatg
     9121 tatctattga aaagacatta gtggcatcaa aacgttggat tagatatatc catgaagttg
     9181 aagagccaga ttttttgatt gtaatttatc atggtggatt gaataaaatt agtaatagta
     9241 cgaaaaataa aaaggcaagt tcgaatgaag ctgaaaaatt aatggaagaa ctcggtgtta
     9301 tagatttaat gattacagct catcagcatc aaacaatagt aggtcaagat catgaaacgt
     9361 attatgttca ggctggtcag gatgccaaag agcttgtaca tctttcgatt aattttaaaa
     9421 agagaacaac aacttatgat gttgaaagca ttgattctaa agtgattgac ttaaatgagt
     9481 atgaagagga tcaagaatta ttagatttaa cattctatga tagaaaagca gtggcttatt
     9541 ggtcacagga aatcataagt gataaaggtt tgatgttatc agtgaatggg ttacaagatt
     9601 tagtctgtca aacacatcca ttttcgcaat tattacatga tgcaattcag cttgcatttg
     9661 ataatgatat aacatgtgtc cacgtgccta tgaacggaga gaaggggttg agtggacaga
     9721 ttcgaaatga agatttgtat catgcatacc catatccaga taagccaatg gatatgacaa
     9781 ttagtggtca aaatatcaaa gatatattgg agtatagtta ttcacattta gattttgtta
     9841 acgagcaatt aagcttaaca attattgatg aaacgttatg tacaatgtgg caaggattca
     9901 attatgagat tgatatgaat caagaacctg ggcaacgagt aatgttagat caaattgatt
     9961 tgactaagag ttatagagtt acaatgactg actattgtta tcgtaactac aagaattatt
    10021 taaaaaatgc tattatacat gaatcgtacg atgaaacaat gagtacatta attgcagaga
    10081 agttaagaga tccgaattat cgtatttcat gtagtgataa ttttgtagtt aaaaacaggt
    10141 aaatattgct atgtaataaa ttaattataa gttcaacaag tataaaaacc tttcttagag
    10201 aagattatct aggaaaggtt tttagaattt taaaattgat ataaaagaac agtgaaattc
    10261 tcattcatta tatataagta aacaaaaaag ctaaaatggt atataccatt ttagcaagta
    10321 acggaaacgg agggattcga accctcgcgc cgctttcgcg acctacaccc ttagcagggg
    10381 cgcctcttca gccaacttga gtacgtttcc aatggctcca caggtaggac tcgaacctac
    10441 gaccgatcgg ttaacagccg atagctctac cactgagcta ctgtggatta atttgtatgt
    10501 gtttgaatat caagcacaaa tattattata acaagattaa gttaatttgc aatagatacc
    10561 ttaaaaaaat atgcagaaca tttacactat atttataaaa cattccgcat attttaaaaa
    10621 taaacaattt atttatcttc aaataaagaa tagcgtgtca atgcttcaaa cacattcaat
    10681 gtggctttat tataatctat tggttggtcg ggattttttg atatcatgcg agat
//
LOCUS       DAHHSC010000076        10536 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.076, whole genome shotgun sequence.
ACCESSION   DAHHSC010000076 DAHHSC010000000
VERSION     DAHHSC010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 10536)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10536)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10536
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.076"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            37..801
                     /locus_tag="NBI57_001468"
     CDS             37..801
                     /locus_tag="NBI57_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCD1822410.1"
                     /translation="MILKFDHIIHYIDQLDRFSFPGDVIKLHSGGYHHKYGTFNKLGY
                     INENYIELLDVENNEKLKKMAKTIEGGVTFATQIVQEKYEQGFKNICLRTNDIEAVKN
                     KLQSEQVEVVGPIQMERDTHKDGKVKWQLLYIMNQDDDEIKPPFFIQWEESDSMRTKK
                     LQKYFQKQFSIETVIVKSKNRSQTVSNWLKWFDMDIVEENDHYTDLILKNDDIYFRIE
                     DGKVSKYHSVIIKDAQATSPYSIFIRGAIYRFEPLV"
     gene            complement(793..1044)
                     /locus_tag="NBI57_001469"
     CDS             complement(793..1044)
                     /locus_tag="NBI57_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1822411.1"
                     /translation="MRTIILSLFIIMNIVAIIMTLSQPLTVNYFSLRVILIFFTFILS
                     IFFILIKSSRLNNILTILSIVLVIIHMGILAHSTYVYLY"
     gene            1433..2698
                     /gene="femX"
                     /locus_tag="NBI57_001470"
                     /gene_synonym="fmhB"
     CDS             1433..2698
                     /gene="femX"
                     /locus_tag="NBI57_001470"
                     /gene_synonym="fmhB"
                     /EC_number="2.3.2.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid II:glycine glycyltransferase"
                     /protein_id="HCD1822412.1"
                     /translation="MEKMHITNQEHDAFVKSHPNGDLLQLTKWAETKKLTGWYARRIA
                     VGRDGEVQGVAQLLFKKVPKLPYTLCYISRGFVVDYSNKEALNALLDSAKEIAKAEKA
                     YAIKIDPDVEVDKGTDALQNLKALGFKHKGFKEGLSKDYIQPRMTMITPIDKNDDELL
                     NSFERRNRSKVRLALKRGTTVERSDREGLKTFAELMKITGERDGFLTRDISYFENIYD
                     ALHEDGDAELFLVKLDPKENIAKVNQELNELHAEIAKWQQKMETSEKQAKKAQNMIND
                     AQNKIAKNEDLKRDLEALEKEHPEGIYLSGALLMFAGSKSYYLYGASSNEFRDFLPNH
                     HMQYTMMKYAREHGATTYDFGGTDNDPDKDSEHYGLWAFKKVWGTYLSEKIGEFDYIL
                     NQPLYQLIEQVKPRLTKAKIKISRKLKRK"
     gene            2815..5982
                     /locus_tag="NBI57_001471"
     CDS             2815..5982
                     /locus_tag="NBI57_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HCD1822413.1"
                     /translation="MIKKLLQFSLGNKFAIFLMVVLVVLGGVYASAKLKLELLPNVQN
                     PVISVTTTMPGATPQSTQDEISSKIDNQVRSLAYVKNVKTQSIQNASIVTVEYENNTD
                     MDKAEEQLKKEIDKIKFKDEVGQPELRRNSMDAFPVLAYSFSNKENDLKKVTKVLNEQ
                     LIPKLQTVDGVQNAQLNGQTNREITLKFKQNELEKYGLTADDVENYLKTATRTTPLGL
                     FQFGDKDKSIVVDGQYQSVDAFKNINIPLTLAGGQGQSQSQSDNKQNSAMSDVNQASP
                     QQNSKASAPNNISGMPTAKLGDLADITVGDVRTSISKTNGKDAVNLQITKAQDANTVQ
                     VAKDVQRKIDTFVDENKDFNVTKTMDTAKPVEKSLYTMVEKASLGTIVAIIVILLFLR
                     NIRTTAISIISIPLSLLMALIALKLSDVSLNILTLGALTVAIGRVIDDSIVVVENIYR
                     RLTDSEEQLKGENLIISATTEVFKPIMSSTLVTIIVFLPLVFVSGSVGEMFRPFALAI
                     AFSLLASLLVSITLVPALAATLFKKGVKRRNKQHQEGLGVVSTTYKKVLHWSLNHKWI
                     VIILSTLILVATIVFGGPRLGTSFISAGDDKFLAITYTPKPGETEQAVLNHAKDVEKY
                     LKQKKHVKTIQYSVGGSSPVDPTGSTNSMAIMVEYDNDTPNFDVEADKVIKHVDGFKH
                     PGEWKNQDLGTGAGNKSVEVTVKGPSMDAIKSTVKDIEQKMKQVKGLANVKSDLSQTY
                     DQYEIKVDQNKAAENGISASQLAMHLNENLPEKTVTTVKENGKTVDVKVKQNKQTDWS
                     EDKLNNITLKKPTGGTIKLGDIATLVKTTTPSKLTQEQGDYATTVSAKVTNKDVGGTT
                     RQVMSKINNLDKPNNVKVNIGGASDDINNAMTQLAFAMLAAIIIVYLILVITFKGGLA
                     PFTILFSLPFTVIGVIIALLITGETISVPSLIGMLMLIGIVVTNAIVLIDRVINNEQQ
                     GMEMKEALIEAGGTRIRPILMTAIATIGALVPLLFGQDSSILISKGLAATVIGGLISS
                     TLLTLVVVPVIYEILFTLKKRFTKR"
     gene            complement(6166..6483)
                     /gene="mspA"
                     /locus_tag="NBI57_001472"
     CDS             complement(6166..6483)
                     /gene="mspA"
                     /locus_tag="NBI57_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane stabilizing protein MspA"
                     /protein_id="HCD1822414.1"
                     /translation="MQFYLILLAILYLIVSFISIFKMEVVFTRILRIIMGVLLLFVLA
                     LTTMSFPKENWWVFIVLLLLVGNVEVTGFKMLKKDLKGVNILNLMSLFIFVIYFILTI
                     VLF"
     gene            complement(6728..6892)
                     /locus_tag="NBI57_001473"
     CDS             complement(6728..6892)
                     /locus_tag="NBI57_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1822415.1"
                     /translation="MSLENQLAELKYDYVRLQGDLEKRESLNLDTSALVRQLKDIENE
                     IRNVRAQMQD"
     gene            7025..7933
                     /locus_tag="NBI57_001474"
     CDS             7025..7933
                     /locus_tag="NBI57_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002487080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HCD1822416.1"
                     /translation="MTEQFTMIILLIALGYFLKRINFIKATDSQVIATLVLNVTLPSL
                     VIVNLNSAELKLSFSILPILMIIYGIVAKMIAVWFFRKYDNHMRGSVGMMTGAMNIGL
                     FAYPLIEVIWPKTGLIYFGMADIGGAFVMFGITYFVGSYFSEGSDQFDFKYLGKKLLQ
                     SVPLVTYIVMFILNINHIHLPHVAIDFFSILSHANMPLSMILLGVMLNFTLERKYIPA
                     TIKYLCLHYGLALVAGLLVHVLLPVSDDMIKTTLLVTWMFPVGVAIISYGIQFKYRTL
                     PFIGMTTNLTIIISIIILYVYQAVFV"
     gene            8340..8471
                     /locus_tag="NBI57_001475"
     CDS             8340..8471
                     /locus_tag="NBI57_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1822417.1"
                     /translation="MYGAYLINEYHQDFKSIANFKVLKRMINVMMCYCVLFLEPRIS"
     gene            complement(8588..9451)
                     /locus_tag="NBI57_001476"
     CDS             complement(8588..9451)
                     /locus_tag="NBI57_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake protein GlcU"
                     /protein_id="HCD1822418.1"
                     /translation="MQFLDFLIALLPALFWGSVVLINVFVGGGPYNQIRGTTLGALIV
                     GLGLLITGFAKFNNPTVIIVGLISGALWAFGQANQLKSISLIGVSNTMPVSTGMQLVG
                     TTLFSVIFLGEWSSMTQIIFGLIAMILLVTGVALTSLKAKNERQSDNPEFKKAMGILI
                     VSTVGYVGFVVLGDIFGVGGTDALFFQSVGMAIGGFILSMNHKTSLKSTALNLLPGVI
                     WGIGNLFMFYSQPKVGVATSFSLSQLLVIVSTLGGIFILGERKDRRQMTGIWAGIIII
                     VIAAIILGNLK"
     gene            complement(9632..10519)
                     /locus_tag="NBI57_001477"
     CDS             complement(9632..10519)
                     /locus_tag="NBI57_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCD1822419.1"
                     /translation="MSIVQLYDITQIKSFIEHSNYESASYLYKLPQQYNEIDVLITDA
                     IESPGVFSIKENDSIKAIILSFAYDKNKFKVIGPFVADNYVLSVDTFETLFKAMTSNQ
                     PDDAVFNFSFEEGIQQYKPLMKVIQASYNFTDYYIEARTRLEEDMHQPNIIPYHKGFY
                     RAFSKLHTTTFKYQAQSPQDIIDSLDDHHHLFLFVSEGLLKGYLYLEIDSQQSIAEIK
                     YFSSHVDYRLKGIAFELLAYALQYAFDNFDIRKVYFKIRNKNNKLIERFNGLGFHINY
                     EYIKFKFESRNVKDQTIPE"
BASE COUNT         3803 a         1531 c         1921 g         3281 t
ORIGIN      
        1 aaataagcgt aattacatgt aaataggggg atactaatga tattgaaatt tgatcacatc
       61 attcattata tagatcagtt agatcggttt agttttccag gagatgttat aaaattacat
      121 tcaggtgggt atcatcataa atatggaaca ttcaataaat taggttatat caatgaaaat
      181 tatattgagc tactggatgt agaaaataat gaaaagttga aaaagatggc aaaaacgata
      241 gaaggtggag tcacttttgc tactcaaatt gttcaagaga agtatgagca aggctttaaa
      301 aatatttgtt tgcgtacaaa tgatatagag gcagttaaaa ataaactaca aagtgagcag
      361 gttgaagtag tagggccgat tcaaatggaa agagatacac ataaagatgg taaggtaaag
      421 tggcaattgc tttatataat gaatcaggat gatgatgaaa ttaagccacc attttttatt
      481 caatgggaag aaagtgattc catgcgtact aaaaaattgc aaaaatattt tcaaaaacaa
      541 ttttcaattg aaactgttat tgtgaaaagt aaaaaccgat cacaaacagt atcgaattgg
      601 ttgaaatggt ttgatatgga cattgtagaa gagaatgacc attacacaga tttgatttta
      661 aaaaatgatg atatttattt tagaattgaa gatggtaaag tttcaaaata tcattcggtt
      721 atcataaaag acgcacaagc aacttcacca tattcaattt ttatcagagg tgctatttat
      781 cgctttgaac cattagtata aatatacgta agtgctatga gcgagaatgc ccatatgaat
      841 aatgacaagc acaatggaaa gaatcgttaa tatattattt aatcgggatg acttaattaa
      901 aatgaaaaag attgataata taaatgtgaa aaagataagt ataacccgta aactaaagta
      961 attcacggtg agaggttgac tcaatgtcat aatgattgca acgatgttca taattataaa
     1021 tagacttaaa ataattgttc tcatatcaaa cacctcattg ttagattatt gacattataa
     1081 caggggtaat tgtatatgaa cattaatgtg gttgcttgag gaaaaattta ttcattgaag
     1141 tcaagttggt tcattttaga aatgaatatc gtgttagatg atgaaagtat attgaagtat
     1201 aggtaactag ttgaaaagta ttaattgtac gataacatta aatttaacac gaaacataga
     1261 tataaaatga ttcacaatta aaatgggtaa atttgaactt gctaaactat taattggagc
     1321 atggacattt caaaaataag agttcaaatc ttacacaagc tctgaatcga cactataaga
     1381 tacaaactgt ataattaaag gtattgttaa atagaaggag atatcataaa tcatggaaaa
     1441 gatgcatatc actaatcagg aacatgacgc atttgttaaa tctcacccaa atggagattt
     1501 attacaatta acgaaatggg cagaaacaaa gaaattaact ggatggtacg cgcgaagaat
     1561 cgctgtaggt cgtgacggtg aagttcaggg tgttgcgcaa ttacttttta aaaaagtacc
     1621 taaattaccg tatacgctat gttatatttc acgtggtttt gttgttgatt atagtaataa
     1681 agaagcgtta aatgcattgt tagacagtgc aaaagaaatt gctaaagctg agaaagcgta
     1741 tgcaattaaa atcgatcctg atgttgaagt tgataaaggt acagatgctt tgcaaaattt
     1801 gaaagcgctt ggttttaaac ataaaggatt taaagaaggt ttatcaaaag actacatcca
     1861 accacgtatg actatgatta caccaattga taaaaatgat gatgagttat taaatagttt
     1921 tgaacgccga aatcgttcaa aagtgcgctt ggctttaaag cgaggtacga cagtagaacg
     1981 atctgataga gaaggtttaa aaacatttgc tgaattaatg aaaatcactg gggaacgcga
     2041 tggcttctta acgcgtgata ttagttactt tgaaaatatt tatgatgcgt tgcatgaaga
     2101 tggagatgct gaactatttt tagtaaagtt ggacccaaaa gaaaatatag cgaaagtaaa
     2161 tcaagaattg aatgaacttc atgccgaaat agctaaatgg cagcagaaga tggaaacatc
     2221 tgaaaagcaa gctaaaaaag cgcaaaatat gattaatgat gcgcaaaata aaattgctaa
     2281 aaatgaagat ttaaaacgag acctagaagc tttagaaaag gaacatcctg aaggtattta
     2341 tctttctggt gcactattaa tgtttgctgg ctcaaaatca tattacttat atggtgcgtc
     2401 ttctaatgaa tttagagatt ttttaccaaa tcaccatatg cagtatacga tgatgaagta
     2461 tgcacgtgaa catggtgcaa caacttacga tttcggtggt acagataatg atccagataa
     2521 agactctgaa cattatggct tatgggcatt taaaaaagtg tggggaacat acttaagtga
     2581 aaagattggt gaatttgatt atatattgaa tcagccattg taccaattaa ttgagcaagt
     2641 taaaccgcgt ttaacaaaag ctaaaattaa aatatctcgt aaattaaaac gaaaatagat
     2701 taacgactga aatctgaacg ctcataagac tgtcatttgc gttcagattt ttttacacaa
     2761 tatagaatgg ttgagtaaaa tatttttgaa tatagtgaaa gagggggaag tactgtgata
     2821 aaaaagctat tacaattttc tttggggaat aagtttgcta tctttttaat ggttgtttta
     2881 gttgtcttgg gcggtgtata cgcgagtgct aaattgaaat tagaattact accaaatgta
     2941 caaaatccag ttatttcagt tacaacaaca atgccgggtg caacgccaca aagtacccaa
     3001 gatgaaataa gtagtaaaat tgacaatcaa gtaagatcgt tggcatatgt gaaaaatgtt
     3061 aaaacgcaat ccatacaaaa tgcttcaatt gtaacagttg aatatgaaaa taatacagat
     3121 atggataaag cagaagaaca gcttaaaaaa gaaatcgata aaattaaatt taaagatgaa
     3181 gttggtcaac cagaattaag acgtaattcg atggacgctt ttccggtttt agcatattca
     3241 ttttcaaata aagagaatga cttgaaaaaa gtaacgaaag tactgaatga acaattaata
     3301 ccaaaattac aaacggtaga tggtgtgcaa aatgcgcaat taaatgggca gacgaaccgt
     3361 gaaatcaccc ttaaatttaa gcaaaatgaa cttgaaaaat atgggttgac tgctgatgat
     3421 gtagaaaact atctaaaaac agcaacaaga acaacaccac ttggattgtt ccaatttggt
     3481 gataaagata aatcaattgt tgttgatggt caatatcaat ctgttgatgc ttttaaaaac
     3541 ataaatattc cattaacgtt ggcaggaggg caagggcaat ctcaatccca aagtgataat
     3601 aaacaaaatt cagccatgtc agacgttaat caggcatcac cgcagcaaaa ttcaaaagcg
     3661 tcagcaccaa ataatataag tggtatgccg acagcgaaac taggagattt agctgatatt
     3721 acagttggtg atgtgcgtac ttctatttct aaaacgaatg gaaaggatgc ggttaatcta
     3781 caaataacta aagctcaaga tgccaataca gttcaagtag ccaaagatgt acaacgtaaa
     3841 attgatacat tcgttgatga aaataaagat tttaatgtca caaaaacaat ggatactgca
     3901 aagcctgttg agaaatcact ttatacgatg gttgaaaaag catcattagg tacaatcgtg
     3961 gcaattatag ttattttgct gtttttaaga aacattcgta cgacggcaat ttctattata
     4021 tccattccgt tatcacttct tatggcgctt attgctctga aattgagtga tgtttcattg
     4081 aatatactaa cgttaggtgc attaacagta gcgattggac gtgtgataga cgattcgatt
     4141 gtagttgttg aaaatattta tcgacgctta acagattcag aagaacaact aaaaggtgaa
     4201 aatttaatta tcagtgcgac aactgaagta tttaaaccaa taatgtcatc gacactagtt
     4261 actattatcg tcttcttacc acttgtgttt gtatcaggtt cagtaggcga aatgtttaga
     4321 ccttttgcat tggctattgc atttagttta ttagcatcgt tattagtgtc aattacactc
     4381 gttccagctt tggcagcaac actatttaaa aaaggcgtta aacgtcgtaa taaacaacat
     4441 caagaaggat taggtgttgt tagtacaact tataaaaaag tattgcattg gtcactaaat
     4501 cataagtgga ttgtaattat attaagtaca ttaattttgg ttgcaactat tgtatttgga
     4561 ggaccgagac taggcactag ctttatttca gcaggtgacg ataaattttt agctattact
     4621 tatacaccga agcctggtga aacggagcaa gcagtgttga atcatgcgaa agatgttgaa
     4681 aaatatttaa aacagaaaaa gcatgtaaaa acaattcaat actcagttgg tggtagtagt
     4741 ccagtagatc caacgggtag tacaaatagt atggcaatca tggttgaata tgataatgac
     4801 acgcctaatt ttgatgtaga agcggataag gttattaaac atgtagatgg ctttaaacat
     4861 cctggagagt ggaaaaatca agatttagga acaggtgcag gtaataaatc tgtagaggtt
     4921 actgtgaaag gcccatcaat ggatgccata aaatcaactg taaaagatat tgaacagaaa
     4981 atgaaacagg ttaaaggact agccaatgtc aaatctgatt tatcgcaaac atatgatcag
     5041 tatgaaatta aagttgatca aaataaagcg gcagaaaatg gtatttctgc aagtcaactt
     5101 gcaatgcact tgaatgaaaa cttaccagaa aaaacagtta cgactgttaa agaaaatggt
     5161 aaaactgttg atgttaaagt caaacaaaat aagcaaacag actggtcaga agataagttg
     5221 aataatataa ctttgaaaaa gccgactggt ggtacgatta aattgggaga tatcgctacg
     5281 ttagttaaaa caacgacacc aagtaaattg acgcaagaac aaggtgatta tgcaacgacg
     5341 gtatctgcta aagtaacaaa taaagatgtg ggtggcacaa cacgacaagt gatgtctaaa
     5401 ataaataatt tggacaaacc gaataatgta aaggttaata tcggtggtgc atcagatgat
     5461 attaacaatg caatgacaca attagccttt gcaatgttag ctgcaatcat tatcgtatat
     5521 ttaatcctag ttattacatt taaaggtggc ttagcgccat ttacaatttt attctcttta
     5581 ccatttacag ttatcggtgt aattattgca ctattaatca caggagaaac aatatcagta
     5641 ccaagtttaa ttggtatgct aatgttaatt ggaatcgtag taacaaatgc cattgtgtta
     5701 atagaccgtg tcattaataa tgagcagcag ggcatggaga tgaaagaagc attaatcgaa
     5761 gcaggcggta ctagaattag accgatatta atgacggcga ttgcaacaat cggtgcatta
     5821 gttcctttgt tatttggtca agatagctcg attcttattt cgaaaggatt agctgctaca
     5881 gttattggtg gtttaatttc atcaacttta ttaacacttg tagttgtacc agtaatatat
     5941 gaaattctct ttactttaaa aaaacgattc actaaacgat agataatata aaagccataa
     6001 aagcggtcat gatattgact acttttatgg ctttttaaaa tttgaaattt tagaccttat
     6061 ttcacgtgcg atttaatcga tgtgttaata aaacaatact ggcattcaac atgtacgata
     6121 ataaaataaa attgcaatta tgttattgca tgttttgttt attatttaga ataatacgat
     6181 ggttaagatg aaatatatga caaagataaa taatgacatt aaattcaaga tgtttacacc
     6241 ttttagatct tttttaagca ttttaaatcc tgtcacttcg acattgccga ctaagagtag
     6301 taagacgata aatacccacc aattctcttt tggaaaactc atcgtcgtta atgctaagac
     6361 gaacaataac aacacaccca taataattct caaaatgcga gtaaatacaa cttccatttt
     6421 aaaaatacta ataaaactaa caattagata aagtattgct agtaaaatca gataaaattg
     6481 catatgataa cttcctctcc gtttcaaagg gttcatgtag tacagcagca tagaaaacat
     6541 aaatagcagt gttgggatag atatgcgtgt atgttgttaa tgaaacacta aatcataaca
     6601 cgacaacatc ttgtgattct agatgtaatt gagcgaaaaa taaagcaatt catcaatata
     6661 acgtattcag agcaatgcta gtcaaattta tgtaacgaaa agcatttatt gcttgaatga
     6721 tagattatta atcttgcatt tgagcgcgaa cgtttctaat ttcattttca atatctttaa
     6781 gttgacgaac aagtgcggaa gtatctaaat tcaaagattc ccgtttttct aagtcacctt
     6841 gaagacgaac ataatcatat ttaagttcgg ctagttggtt ttctaaagac atactataac
     6901 actcctttga aatataatta agtgcattta atgtagatta tagcatgaaa cagtagactt
     6961 ctaaaatatc tgtggagtat gttaaagtta taaattatga agattattga aagaagggat
     7021 aacagtgaca gaacaattta ctatgattat tttattaatt gcgcttggtt actttttgaa
     7081 gcgcattaat tttataaagg caacagatag tcaagtcatt gctacattag ttcttaatgt
     7141 cacactccca tctttagtta ttgtgaattt aaacagtgca gaattaaaat tgtcattctc
     7201 cattttacca attttaatga ttatatatgg tattgtggcg aaaatgattg ctgtctggtt
     7261 ttttagaaaa tatgataatc acatgcgtgg atcagtaggt atgatgactg gtgctatgaa
     7321 tattggattg tttgcatatc cactaattga agtgatatgg cctaaaacgg ggcttattta
     7381 ttttggtatg gctgatatag gtggtgcctt tgtgatgttt ggaattactt actttgtagg
     7441 tagctacttt agtgaaggta gtgatcaatt cgattttaaa tatttaggta agaaattatt
     7501 acagtcagtg ccactcgtta catacattgt aatgtttata ttgaatataa atcacatcca
     7561 tttaccacat gtggcaatcg atttctttag catactttcc catgcaaata tgccgttatc
     7621 aatgattttg ctaggtgtta tgttgaattt tactctggaa agaaagtata tacctgctac
     7681 tattaaatat ttatgtttgc attatggatt agcattagtt gcgggattgt tagtacatgt
     7741 gttgttaccg gtatcagatg atatgattaa gacgacattg ttggtcactt ggatgttccc
     7801 ggttggtgtc gcaattattt cttatggtat tcagtttaaa tatcgaacac tgccatttat
     7861 aggtatgacg accaatttga caatcattat tagtatcatt attttgtacg tgtatcaggc
     7921 agtgtttgtc tagtttacac atttgaaaat aaaatccatt ttagtattcg tagaaaatga
     7981 gagccccctt taccacaaca tgtaaatgca tattgtgaaa agggggcttt tatttatgac
     8041 atagttgttc aacaggccgg gacataaatc aatattctat gctctacgaa gttatattgg
     8101 cagtagttta ctgaacgaaa atgcgcttgt aacaagcttt tttcaattct agtcaggggc
     8161 cccaacacag aagctgacga aaagtcagct tacaataatg tgcaagttgg ggaggggccc
     8221 caacaaagag aaattggatt cccaatttct acagacaatg caagttgggg tgggacgacg
     8281 aaatatattt tgcgaaaata tcatttctgt cccactccct atgcagctat ccacttcata
     8341 tgtatggcgc atacttgata aatgaatatc atcaagattt taaaagtatc gctaatttta
     8401 aagtcttaaa acgcatgatt aatgtcatga tgtgttattg cgttttgttt ttagagccac
     8461 gtatttccta ggacatatag aggaacattc aacgtaaaat tcccggaaat taattttaat
     8521 cattctataa gtgaatggtt attttcagaa gtgattacat ttttacgtta catgagtatt
     8581 taactttcta tttcaaatta cctagaatta tagcagctat cacgataata ataatacctg
     8641 cccaaatacc cgtcatctga cgacgatctt ttctttctcc taaaatgaaa ataccaccta
     8701 atgttgaaac gataacaagt aactgtgata atgagaaact tgtagctaca ccaacttttg
     8761 gttgagaata gaacatgaac aagttaccaa ttccccaaat cacacctggc aatagattaa
     8821 gtgctgttga tttaagtgat gttttatgat tcatggatag gataaagcca ccaattgcca
     8881 taccgacaga ttggaagaac aatgcatcag ttccaccaac accaaagatg tcaccaagta
     8941 caacgaaacc tacatatcca actgtagata caattaaaat acccattgct tttttaaatt
     9001 caggattatc tgattgacgt tcatttttgg ctttaagtga agtaagtgct acaccagtaa
     9061 ctaataatat catggcgatt aaaccaaaga taatttgagt cattgaagac cattcaccta
     9121 aaaagataac gctgaataat gttgtaccaa ctaattgcat acctgtagaa actggcatag
     9181 tatttgatac accgattaaa ctaatagatt taagctgatt cgcttgtcca aacgcccata
     9241 atgcaccaga aataagaccg acaataatta cagtaggatt attgaatttt gcgaaaccag
     9301 taataagtaa acctaatcca acaatgagtg cgcctagcgt cgtaccacga atttggttgt
     9361 aaggtccacc gccgacgaac acattaataa gaacgacact tccccagaat aaagcaggta
     9421 aaagtgcgat taagaaatca agaaattgca agactactca ctctcctata ctatttttgc
     9481 aagtgctccc cttaattgac acttgcatgt tattgtaaag cattctgccc taatagtaaa
     9541 aaacaactac ttataatttt caatatttac aaaaagtaga aaaacccaca atacatgcta
     9601 atgaagtact gtggggtatt tgcttggtat tttattcagg gattgtttga tcttttacgt
     9661 tacgtgattc gaatttgaat ttaatgtact catagttgat atggaaacct agaccattaa
     9721 atcgttcgat gagtttatta tttttattac gaattttaaa ataaactttt ctaatatcaa
     9781 aattatcaaa agcatattgc aatgcatacg caagcaactc gaaagcgata cctttcaaac
     9841 ggtaatctac atgagaactg aagtatttaa tctcggcgat tgactgttgt gaatcaattt
     9901 caaggtataa ataaccttta agtaaacctt cgctaacaaa taaaaacaaa tgatgatggt
     9961 cgtctaaact atcaatgata tcttgcggtg actgtgcctg atatttaaat gtagttgtgt
    10021 gtaatttgct gaaagcacga taaaaccctt tgtgataagg aatgatattt ggttggtgca
    10081 tatcttcttc taatcttgta cgggcttcta tgtaatagtc agtgaagtta taacttgctt
    10141 gaataacttt cattaatggt ttgtattgtt gaatgccttc ttcaaaagaa aagttaaaga
    10201 cggcatcgtc aggttggttc gaagtcattg ctttaaatag cgtttcaaac gtatcgacag
    10261 ataatacata attgtcagcc acgaatgggc ctatgacttt gaatttattt ttatcgtatg
    10321 caaaagacaa tatgattgct ttgattgaat cgttttcttt aatcgaaaat acaccaggtg
    10381 attcaatcgc atcggttatt aatacatcta tttcattgta ctgttgagga agtttatata
    10441 agtatgatgc tgattcataa ttcgaatgtt caatgaacga ttttatttgt gtaatatcat
    10501 ataactgaac tatgctcatg tcattacctc cgtact
//
LOCUS       DAHHSC010000077        10523 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.077, whole genome shotgun sequence.
ACCESSION   DAHHSC010000077 DAHHSC010000000
VERSION     DAHHSC010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 10523)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10523)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10523
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.077"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            complement(189..1364)
                     /locus_tag="NBI57_001478"
     CDS             complement(189..1364)
                     /locus_tag="NBI57_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001625397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HCD1822420.1"
                     /translation="MFIGKNLEYVRKLNALSRKELSEKINVSEQAIWQYETKNMMPEI
                     SKIYDMTSIFNVKSSYFISEQPEELLINSVDKHSIAFRAKNYKVSTKLLNKQYYQAMY
                     LSNLTSYLFSFVTIPDNIILSLINNLDELLNGNLESLNKKESIKEIAKVVRAKILQDE
                     SNEALLFMLEKAGIVIYEKRINDSIDAYSFWSKDQTPFIILGTNRGVAVRRNFDLAHE
                     LGHLVLHRHIQFDLLSPEEYKTIEHEADIFASEFLLPEEAFKKDFDQMTKKSNPDYLA
                     VLKEKWYVSIQAIAMRAYYLGLMSSTQYRYFWASLNKKGYKSKEPLDDVIEMSRPVKM
                     NSLLKLYFDRNILTPQKLLNYLKVDETFLNHLAGINLKLFKDYVNENREYNITNLYK"
     gene            complement(1370..2137)
                     /locus_tag="NBI57_001479"
     CDS             complement(1370..2137)
                     /locus_tag="NBI57_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016170495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1822421.1"
                     /translation="METIKQEVRESIVKGIIAGYKDYVNERNEVKKRMVISDAYAFTK
                     SNHIESQVAKHLENFVKYIKENAGPSWKYLKFIFNKDEEKNNIMFILKNEDYFDEKNV
                     SVGKSLVADKNPKSKNYLEVLMAKNRDINFGTVGEDFEIGHQMTADSILFNIKEGSNR
                     DINSYFLIITYRIDKESKQLAAIKQWLPNPETNSAIMVDDLTELIEKVIVEREDYHID
                     EEELEVLKNDGELELIDVEHAFGISIDEGNDMIESER"
     gene            complement(2294..3829)
                     /locus_tag="NBI57_001480"
     CDS             complement(2294..3829)
                     /locus_tag="NBI57_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HCD1822422.1"
                     /translation="MSNIAFYVVSDVHGYIFPTDFTSRNQYQPMGLLLANHVIEQDRR
                     QYDQSFKIDNGDFLQGSPFCNYLIAHSGSSQPLVDFYNQMAFDFGTLGNHEFNYGLPY
                     LKDTLRRLNYPVLCANIYENDSTLTDNGVQYFQVGDQTVGVIGLTTQFIPHWEQPEHI
                     QSLTFHSAFETLQQHLPEMKRHADIIVVCYHGGFEKDLENGTPTEVLTGENEGYAMLE
                     AFSKDIDIFITGHQHRQIAERFKQTAVIQPGTRGTTVGKVVLSTDEYENVSVESCELL
                     PVIDDSTFTIDEDDQHLRKQLEDWLDYEITTLPYDMTINHAFEARVAPHPFTNFMNYA
                     LLEKSDADVACTALFDSASGFKQVVTMRDVINNYPFPNTFKVLAVSGAKLKEAIERSA
                     EYFDVQNDEVSVSADFLGPKPQHFNYDIYGGVSYTIHVGRPKGQRVSNMMIQGHAVDL
                     KQTYTICVNNYRAVGGGQYDMFIDAPVVKDIQVEGAQLLIDFLSNNNLMRIPQVVDFK
                     VEK"
     gene            complement(3880..5424)
                     /locus_tag="NBI57_001481"
     CDS             complement(3880..5424)
                     /locus_tag="NBI57_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HCD1822423.1"
                     /translation="MKKIYKSLTVSAIVATVSLSALPQSLAITHESQPTKQQRTVLFD
                     RSHGQTAGAADWVSDGAFSDYADSIQKQGYDVKAIDGHSNITEASLKSSKIFVIPEAN
                     IPFKESEQAAIVNYVKQGGNVVFISDHYNADRNLNRIDSSEAMNGYRRGAYEDMSKGM
                     NAEEKSSTAMQGVKSSDWLSTNFGVRFRYNALGDLNTSNIVSSKESFGITEGVKSVSM
                     HAGSTLAITNPEKAKGIVYTPEQLPAKSKWSHAVDQGIYNGGGKAEGPYVAISKVGKG
                     KAAFIGDSSLVEDSSPKYVREDNGEKKKTYDGFKEQDNGKLLNNITAWMSKDSDGKSL
                     KASGLTLDTKTKLLDFERPERSTEPEKEPWSQPPSGYKWYDPTTFKAGSYGSEKGADP
                     QPNTPDDHTPPNQNVKISFDIPQNVSVNEPFEVTIHLKGFEANQTLENLRVGIYKEGG
                     RQIGQFSSKDNDYNPPGYSTLPTVKADENGNATIKINAKVLESMEGSKIRLKLGDKTL
                     ITTDFK"
     gene            5653..6609
                     /locus_tag="NBI57_001482"
     CDS             5653..6609
                     /locus_tag="NBI57_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate/phosphite/phosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HCD1822424.1"
                     /translation="MKNFKYLFVLMLAVIVFAAACGNSSSLDNQKNASNDSDSKSGGY
                     KPKELTVQFVPSQNAGTLEAKAKPLEKLLSKELGIPVKVSVSTNYNTIVEAMKSKKVD
                     VGFLPPTAYTLAHDQKAADLLLQAQRFGVKEDGSASKELVDSYKSEILVKKDSKIKSL
                     KDLKGKKIALQDVTSTAGYTFPLAMLKNEAGINATKDMKIVNVKGHDQAVISLLNGDV
                     DAAAVFNDARNTVKKDQPNVFKDTRILKLTQAIPNDTISVRPDMDKDFQEKLKKAFID
                     IAKSKEGHKIISEVYSHEGYTETKDSNFDIVREYEKLVKDMK"
     gene            6804..7577
                     /gene="phnC"
                     /locus_tag="NBI57_001483"
     CDS             6804..7577
                     /gene="phnC"
                     /locus_tag="NBI57_001483"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HCD1822425.1"
                     /translation="MSQIEFKNVSKVYPNGHVGLKNINLNIEKGEFAVIVGLSGAGKS
                     TLLRSVNRLHDITSGEIFIQGKSITKAHGKALLEMRRNIGMIFQHFNLVKRSSVLRNV
                     LSGRVGYHPTWKMVLGLFPKEDKIKAMDALERVNILDKYNQRSDELSGGQQQRISIAR
                     ALCQESEIILADEPVASLDPLTTKQVMDDLRKINQELGITILINLHFVDLAKEYGTRI
                     IGLRDGEVVYDGPASEATDDVFSEIYGRTIKEDEKLGVN"
     gene            7579..8379
                     /gene="phnE"
                     /locus_tag="NBI57_001484"
     CDS             7579..8379
                     /gene="phnE"
                     /locus_tag="NBI57_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HCD1822426.1"
                     /translation="MPLEIPTKYDSLLKKKVSLKTSFTFMLIIVLIIWSFIYTGFNFG
                     DLMIGIPQIGDLFKQMIPPDFEYLQQITTPMLDTIRMAIVSTVLGSIVSIPIALLCAS
                     NIVHQKWISIPSRFILNIVRTIPDLLLAAIFVAVFGIGQIPGILALFILTICIIGKLL
                     YESLETIDPGPMEAMTAVGANKIKWIVFGVVPQAISSFMSYVLYAFEVNIRASAVLGL
                     VGAGGIGLFYDQTLGLFQYPKTATIILFTLVIVVVIDYISTKVRAHLA"
     gene            8376..9191
                     /gene="phnE"
                     /locus_tag="NBI57_001485"
     CDS             8376..9191
                     /gene="phnE"
                     /locus_tag="NBI57_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HCD1822427.1"
                     /translation="MTQEIAKYNVHTKAHKRKLIKRWLIAIVVLAIIIWAFAGVPSLE
                     LKSKSLEILKSIFSGLFHPDISYIYIPDGEDLLRGLLETFAIAVVGTFIAAIICIPLA
                     FLGANNMVKLRPVSGVSKFILSVIRVFPEIVMALIFIKAVGPGSFSGVLALGIHSVGM
                     LGKLLAEDIEGLDFSAVESLKASGANKIKTLVFAVIPQIMPAFLSLILYRFELNLRSA
                     SILGLIGAGGIGTPLIFAIQTRSWDRVGIILIGLVLMVAIVDLISGSIRKRIV"
     gene            complement(9320..10498)
                     /gene="deoB"
                     /locus_tag="NBI57_001486"
     CDS             complement(9320..10498)
                     /gene="deoB"
                     /locus_tag="NBI57_001486"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HCD1822428.1"
                     /translation="MTRPFNRVHLIVMDSVGIGEAPDAADFKDEGSHTLRHTLEGFDQ
                     TLPNLEKLGLGNIDKLPVVNAVEQPEAYYTKLSEASVGKDTMTGHWEIMGLNIMQPFK
                     VYPNGFPEELIQQIEEMTGRKVVANKPASGTQIIDEWGEHQMKTGDLIVYTSADPVLQ
                     IAAHEDIIPLEELYDICEKVRELTKDPKYLIGRIIARPYVGEPGNFTRTSNRHDYALK
                     PFGKTVLDHLKDGGYDVIAIGKINDIYDGEGVTEAVRTKSNMDGMDQLMKIVKKDFTG
                     ISFLNLVDFDALYGHRRDKPGYAQAIKDFDDRLPELFSNLKEDDLVIITADHGNDPTA
                     PGTDHTREYIPVIMYSPKFKGGHALESDTTFSSIGATIADNFNVTLPDFGKSYLKELK
                     "
BASE COUNT         3336 a         1918 c         1623 g         3646 t
ORIGIN      
        1 ttcaatgaca taatttattc ataattttat atttaattct gaatattctt taatagccta
       61 aaaagcttgt tatatcgcac aaaacacaac attaaaaggt ccttgtcgct acctatcaac
      121 agatgttaca caaaaaaact cgaactgaaa tgatgatctt ttcagctcga gttttaaata
      181 ttggtgcctt atttatatag attcgttata ttatattctc tattttcatt aacataatcc
      241 ttaaagagtt ttaaattaat acctgctaga tgattcaaaa atgtttcatc gacttttaaa
      301 taattcaata atttttgtgg tgtcagtata tttctatcaa aatacaactt taataaacta
      361 ttcattttga caggacgtga catttcaatc acgtcgtcta aaggctcttt acttttatag
      421 ccttttttat ttaatgatgc ccaaaaatat ctgtactgag tactagacat taggcctaga
      481 taataagctc tcattgcaat tgcttgtata gaaacatacc atttttcttt caatacagct
      541 aaataatcag gatttgattt tttggtcatt tgatcaaagt cttttttaaa cgcttcttca
      601 ggtagtaaaa attcagaggc gaaaatatct gcctcatgtt caattgtctt atattcttca
      661 ggagataata agtcgaattg aatatgacga tgtagaacga gatgtcccag ctcatgtgca
      721 agatcaaaat tccttctaac tgctaccccc ctatttgtac ccagtataat aaaaggtgtc
      781 tgatctttac tccaaaaact atatgcgtct atgctatcat taattcgctt ttcgtatata
      841 actatacccg ctttttcaag cataaataat agtgcttcgt tgctttcatc ttgaagaatt
      901 ttagccctca caactttagc aatttctttt atgctttctt ttttatttag tgattctaaa
      961 ttgccattca acaattcatc taaattattt attaaagata aaataatatt atccggtatc
     1021 gttacaaaag aaaataagta gctagttaaa ttagataaat acattgcttg ataatattgc
     1081 ttatttaata gtttcgtaga tactttataa ttttttgctc taaaagctat actatgttta
     1141 tcgacagaat ttattaggag ctcttcgggc tgctcgctaa taaagtagct agatttaaca
     1201 ttaaaaatag atgtcatatc ataaatttta ctaatttctg gcatcatatt ttttgtttca
     1261 tattgccaaa ttgcttgttc actcacattt attttttctg ataattcttt tctacttaga
     1321 gcattaagct ttctcacgta ttctaaattc ttgccaataa acattaactt cacctttcac
     1381 tttcaatcat atcgttacct tcatcgatac tgataccaaa tgcatgttcc acatctatga
     1441 gttctaactc tccatcgttt ttaagtactt ctaactcttc ttcgtcaata tgataatctt
     1501 ctctttctac tattactttt tctattaact ctgtcaaatc atcaaccata attgcagaat
     1561 ttgtttctgg atttggtaac cattgtttga ttgcagctaa ttgctttgat tctttatcaa
     1621 tacgatatgt aataattaaa aaataagaat taatatctct attactgcct tcttttatat
     1681 taaataatat actatctgca gtcatttgat gcccaatttc aaaatcttct ccaacagtac
     1741 caaaattaat gtccctattc ttagccataa gtacctctag gtagttttta ctttttggat
     1801 ttttatcagc aactaaactc ttccctacgc ttacattttt ttcatcaaag taatcttcat
     1861 ttttcaatat aaacatgata ttatttttct cttcatcttt attaaatata aactttaaat
     1921 acttccatga tggtccagca ttttctttaa tatacttaac aaaattttcc aaatgcttag
     1981 caacttgact ttcaatatga ttagatttag taaaggcata ggcatcacta atcaccatac
     2041 gtttcttgac ttcatttctc tcattaacat agtccttata tcccgcgata attcctttaa
     2101 caatagattc tcttacttct tgtttaattg tttccaaatt aatcacttca taatattaag
     2161 ttatataaaa tataagcctc attttttgta aaatcaacat ttttcttaaa gtatattgac
     2221 gtttaagagt ggttagttta aacaaaaaca aaaacttacc cgattaaaat caagtaagtt
     2281 tttcatatat ccgtcacttt tcaactttaa aatcaacaac ttgcgggata cgcatcaaat
     2341 tattatttga taaaaaatca ataagtaatt gtgcgccttc aacttgaata tctttcacaa
     2401 ctggcgcgtc gataaacata tcatactgac caccgccaac tgcacgataa ttatttacac
     2461 aaattgtata tgtctgcttt aaatcaactg cgtgaccttg aatcatcata ttgctcacac
     2521 gttgtccctt tggtcttcca acatgaatgg tataacttac gccaccatat atatcataat
     2581 taaagtgttg tggtttgggt ccaaggaagt ctgcgctcac acttacttca tcattttgca
     2641 cgtcaaaata ttctgctgat cgttcaatgg cttctttaag tttggcacca cttacagcta
     2701 aaactttaaa tgtatttgga aatgggtaat tgttaataac atctcgcatc gtcacgactt
     2761 gcttgaaacc actagcagaa tcaaacaaag ctgtacaggc aacatctgcg tcactttttt
     2821 ctaataaagc gtaattcata aaattagtaa aaggatgcgg tgccacacgt gcctcaaatg
     2881 catgattaat cgtcatatca tatggcaatg tagtaatttc gtaatctaac cagtcctcta
     2941 actgctttcg taaatgttgg tcatcttcat caatagtaaa tgtggaatca tctataacag
     3001 gaagtaattc acatgattca acggatacat tttcatattc atcagtactc aagacaactt
     3061 tgcctacagt tgtacctctc gtaccaggtt gaatcacagc cgtttgctta aatctttcag
     3121 caatttgtcg atgttggtga cccgtaataa agatatctat atctttagaa aacgcttcta
     3181 acatggcata tccttcattt tcacccgtta atacttcggt cggcgtacca ttttctaaat
     3241 ccttttcaaa tccaccatgg taacaaacca caatgatatc tgcatgtcgc ttcatttcag
     3301 gtaagtgttg ttgaagtgtt tcaaaagcac tatgaaacgt aagtgactga atatgctctg
     3361 gttgttccca atggggaata aattgtgtcg ttaaacctat cacaccaacc gtttgatctc
     3421 caacctgaaa atactgcaca ccgttatcag tcaatgtact atcattttca taaatattag
     3481 cgcacaaaac tggataattg agtctgcgta aagtgtcttt taagtatggc aatccataat
     3541 taaattcatg attaccaagc gtaccaaagt cgaatgccat ttgattataa aaatcaacta
     3601 aaggctggct actgccgcta tgcgcgatta agtaattaca aaatggtgac ccttgcaaaa
     3661 aatcaccatt atctatttta aaactttggt catactgcct tctgtcttgt tctataacat
     3721 gattcgctag taacaatccc ataggttgat attgatttct actcgtaaaa tctgttggga
     3781 aaatataacc atgtacgtcg ctcacgacat aaaatgctat gtttgacatc ctcactcact
     3841 ccttcaatca caaacatctt tcttatttct attatatatt tatttgaagt ctgttgtaat
     3901 caaggttttg tcaccgagtt ttaaacgaat ctttgaacct tccatacttt caagtacttt
     3961 agcattgatc ttaattgtag catttccgtt ttcatctgct ttaactgttg gcaaagtact
     4021 gtaacctggt gggttataat cgttatcttt acttgaaaat tgtccgattt gacgtccgcc
     4081 ttctttgtaa ataccaactc taagattttc aagtgtttga tttgcttcaa atccttttaa
     4141 atgtattgtc acttcaaatg gctcatttac agaaacattt tgcgggatat caaatgatat
     4201 ttttacgttc tgatttggtg gcgtatgatc atctggtgtg tttggctgag gatccgcgcc
     4261 tttttcactg ccataactac ctgctttaaa tgttgttggg tcataccatt tataaccact
     4321 cggcggttgt gaccatggct ctttttcagg ctcagttgaa cgctctggtc gttcaaaatc
     4381 aagcaactta gtctttgtat ctaatgttag tccactcgcc ttaagtgatt tcccatcact
     4441 atctttagac atccaagccg ttatattatt taatagctta ccgttgtctt gttctttaaa
     4501 accatcatat gttttcttct tttctccatt atcttctctt acatatttgg gcgaactatc
     4561 ttccacaagt gatgaatcac cgataaatgc tgctttacct tttccaactt tagaaattgc
     4621 tacatagggg ccttctgctt taccgccacc attataaata ccttgatcta cagcatgtga
     4681 ccatttactt ttcgctggca attgttctgg tgtatacaca ataccttttg ctttctctgg
     4741 attagtaatt gctaatgtcg atccggcatg catagagaca gatttcacac cttcagtaat
     4801 accgaaactt tcttttgaag aaacaatatt gctcgtattt aaatcaccta gtgcattata
     4861 tcgaaaacgt acgccaaagt ttgtagataa ccaatctgaa cttttcacac cttgcattgc
     4921 agtagagctt ttttcttctg cattcatacc tttcgacata tcttcatatg ctccacgtcg
     4981 ataaccattc attgcctccg atgaatcaat acgatttaaa tttcggtcag cattgtaatg
     5041 atctgaaata aagacaacat tgccaccttg tttcacatag ttaacaattg ctgcctgttc
     5101 tgattctttg aaaggaatgt tagcctcagg aattacaaat attttggaac ttttcaaact
     5161 tgcttctgtt atgttcgaat gaccatcaat agctttaacg tcataacctt gtttttgtat
     5221 tgaatccgca taatctgaaa atgcaccatc actaacccaa tctgcagcac cagctgtttg
     5281 accatgagaa cgatcgaata ataccgttcg ctgttgcttt gtaggttgcg attcatgcgt
     5341 tatagctaaa gattgcggta aagcacttaa tgataccgtt gcaacaattg cagagacagt
     5401 taatgactta tatatttttt tcattttgtg aggctccttt taaaataaat ttgttcttga
     5461 attataggat aaaaatccgt tgcatatgag caatttaacg aaaagtttac aaaatcttat
     5521 caaactctta aagaaagtta ttaaaattca tttttataaa atactttttc acatttaaat
     5581 gtggtacgct ataagtgtaa tttcattgca tacatattac acgattaaga atgtgaaggg
     5641 gacagttatc aaatgaaaaa ttttaagtat ttatttgtat taatgttagc agtcattgtt
     5701 tttgcagcag catgtggaaa ctcaagttct ttagataatc aaaagaacgc tagtaatgat
     5761 tcggattcta aatcaggagg atacaaacct aaagaattaa cagtacaatt tgtaccttcg
     5821 caaaatgctg gaacattaga ggctaaagca aaaccattag aaaaattact atctaaagaa
     5881 ttaggaattc cagttaaagt gtctgtatca actaactaca atacaattgt tgaagctatg
     5941 aagtctaaaa aagttgatgt tggtttctta ccaccaacgg catacacatt agcacatgat
     6001 caaaaagcag ctgatttatt attacaagca caacgttttg gtgtaaaaga agatggttca
     6061 gcaagtaaag aacttgtaga tagttataaa tcagaaattc ttgttaaaaa agactcaaaa
     6121 attaaaagct tgaaagattt aaaaggtaag aaaattgcct tacaagatgt aacatcaact
     6181 gctggatata cattcccact tgcgatgtta aaaaacgaag caggtattaa tgcaactaaa
     6241 gatatgaaaa ttgtgaatgt taaaggtcat gaccaagcag ttatctcatt attaaatgga
     6301 gatgtagatg ctgcggctgt atttaacgat gcacgtaata ctgtgaaaaa agaccaacca
     6361 aatgtattta aagacacacg aattttaaaa ttaacacaag ctattccgaa tgacacaatt
     6421 tctgtaagac cagatatgga taaagatttc caagaaaaat tgaaaaaagc ttttatagac
     6481 attgctaaat caaaagaagg tcacaaaatt attagcgaag tttattcaca tgaaggatac
     6541 acagaaacga aagattcaaa tttcgacatt gtaagagagt acgaaaaatt agttaaagat
     6601 atgaaataat cattatcgaa tttggtatta aaagctttcg ttcaatagat atattctaga
     6661 taaatattga aaagctaggc gctaaactga aacagatata gaaaggtgtc gctgtacatt
     6721 tgaaaccatt tgtacacaga aacccaatgt ctatgatatt tcagtttacc ttggcttttc
     6781 tttattaaag aaaggtgtca aacatgagtc aaatcgaatt taaaaacgtc agtaaagtct
     6841 atcctaacgg tcatgtaggc ttgaaaaata ttaacttaaa tattgaaaaa ggtgaatttg
     6901 cagttattgt cggactatct ggtgctggga aatccacgtt attaagatct gtaaatcgtt
     6961 tgcatgatat cacgtcaggt gaaattttca tccaaggcaa atccatcact aaagcccatg
     7021 gtaaagcatt attagaaatg cgccgaaata taggtatgat tttccaacat tttaatttag
     7081 ttaaacggtc aagtgtatta cgaaatgtac taagtggacg tgtaggttat caccctactt
     7141 ggaaaatggt attaggttta ttcccaaaag aagacaaaat taaagcaatg gatgcactag
     7201 aacgcgtcaa tatcttagat aaatataatc aacgctctga tgaattatca ggtggccaac
     7261 aacaacgtat atctattgca cgtgcgctat gccaagaatc cgaaattatt cttgcagatg
     7321 aaccagttgc ttcattagac ccattaacaa cgaaacaggt tatggatgat ttaagaaaaa
     7381 tcaaccaaga attaggcatc acaattttaa ttaatttaca ttttgttgac ttggcaaaag
     7441 aatatggcac acgcatcatt ggtttacgtg atggtgaagt tgtctatgat ggtcctgcat
     7501 ctgaagcaac agatgacgta tttagtgaaa tatatggacg tacaattaaa gaagatgaaa
     7561 agctaggagt gaactaacat gcctttagaa atacctacaa agtatgactc ccttttaaag
     7621 aaaaaggttt ctttaaaaac gagttttacc ttcatgttaa tcattgtgct tatcatttgg
     7681 agcttcattt atacggggtt taattttgga gatttaatga taggaatacc tcaaataggt
     7741 gatctattca aacaaatgat tccacctgat ttcgagtatt tacaacaaat tacaacgcca
     7801 atgttagata ccattcgaat ggctatcgta agtacagtat taggtagcat cgtttcaata
     7861 ccaattgcgt tattatgtgc tagcaatatc gttcatcaaa agtggatttc aataccctcg
     7921 cgctttattt taaatatagt tcgtactatt ccagatttgt tattagcagc aatctttgtg
     7981 gctgtatttg gaatcggtca aattccaggg atattagcat tgtttatttt aactatctgt
     8041 attattggaa aattattata tgaatcattg gaaacgatag atccaggtcc aatggaagca
     8101 atgacggctg ttggcgctaa taaaataaaa tggattgttt tcggtgttgt accacaagcc
     8161 atatcgtcat ttatgtcata cgtattatat gcatttgaag taaatatacg agcttcagct
     8221 gtgcttggat tagtcggcgc tggcggtatt ggattgtttt atgatcaaac acttggttta
     8281 tttcaatatc caaaaacagc aacgattatt ttatttactt tagttatcgt cgtcgtcatt
     8341 gattacatca gtacgaaagt gagggcacat ctcgcatgac acaggaaata gcaaaatata
     8401 atgttcacac aaaagcacac aaacgaaaat tgattaaaag atggcttatt gcaattgtcg
     8461 tcttagctat tatcatctgg gcatttgcag gtgtaccaag tttagaactt aaaagtaaat
     8521 cattagaaat cttaaaatcc atattcagcg gattattcca tcctgacatc agctatatct
     8581 atataccaga tggcgaagac ttattacgtg gtttacttga aacctttgcg atagccgttg
     8641 taggtacttt catcgccgca attatctgta ttccattagc atttttaggt gcaaataata
     8701 tggtaaagtt acgcccggtt tcaggtgtta gcaaatttat tttaagtgtt atacgtgtct
     8761 tcccagaaat tgtaatggca cttatattta tcaaagctgt tggcccaggt tcattttcag
     8821 gtgtattagc tttaggtatc cattctgtag gtatgcttgg gaaactttta gctgaagata
     8881 ttgaaggtct agatttcagt gctgtagaat cattaaaggc cagtggtgcg aataagatta
     8941 aaacactcgt atttgcagtc ataccacaaa ttatgcctgc ctttctatca ctcatacttt
     9001 atcgctttga actaaactta cgttcagctt ctatactggg gctaattggg gctggtggta
     9061 tcgggacacc actcatattt gccattcaaa cacgttcttg ggaccgtgta ggtattatat
     9121 taatcggttt agtactaatg gtcgcaattg tcgatttaat ttccggttca atccgaaaac
     9181 gtattgttta acattaaatc aggatactcc taaataagag gtcctaccgt cacgtttctc
     9241 tattataata aaaacagcag tgaagaaaac tattgttata gttaacttca ctgctgtttt
     9301 tataatatct aaatttattc tatttcaatt cctttaaata acttttaccg aaatctggta
     9361 atgttacgtt gaaattatct gctatagttg caccgataga actgaatgta gtatcacttt
     9421 ctagtgcatg accaccttta aatttcggac tgtacataat tactgggata tattctctcg
     9481 tatggtccgt acctggcgct gtcgggtcat taccatggtc tgctgtaata attactaaat
     9541 cgtcttcttt taagttgcta aacagttctg gcaagcgatc atcgaaatct ttaattgctt
     9601 gtgcataacc tggtttatca cgacgatgac cgtataatgc atcaaagtct actaagttta
     9661 agaagctaat acctgtgaaa tctttcttaa caattttcat caattgatcc ataccgtcca
     9721 tgttactctt cgtacgaacc gcttctgtta caccttcacc atcataaatg tcattaattt
     9781 taccgatggc aataacatca taaccaccgt ctttcaaatg atctaagaca gttttaccaa
     9841 atggttttaa cgcatagtca tgtcgattag atgtacgtgt aaagtttcct ggttcaccaa
     9901 catatggacg tgcgataata cgaccaatta aatatttagg gtcttttgtc aactcacgaa
     9961 ccttttcaca aatatcatat aactcttcta atgggataat gtcttcatgt gcagcaattt
    10021 gcaatactgg gtctgcactt gtataaacaa ttaagtcacc agttttcatt tggtgctcgc
    10081 cccactcatc gataatttgc gtacccgatg ccggtttgtt agcaacaact ttacgacctg
    10141 tcatttcttc aatttgttga attaactctt cagggaatcc attagggtat actttaaaag
    10201 gttgcataat atttaatccc ataatttccc agtgaccagt cattgtatct ttaccaactg
    10261 aagcttcact caatttagta tagtatgctt ctggttgttc aactgcattt actactggta
    10321 atttatcgat gttccctaga cctaactttt caaggtttgg taaagtttga tcgaaacctt
    10381 ctaaggtatg tcttaaagta tgtgaacctt catctttaaa atcagctgcg tctggcgctt
    10441 caccaatacc tactgaatcc attacgatta aatgtacacg attaaatggt cttgtcatag
    10501 ctatcactcc caaattttat ata
//
LOCUS       DAHHSC010000078        10514 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.078, whole genome shotgun sequence.
ACCESSION   DAHHSC010000078 DAHHSC010000000
VERSION     DAHHSC010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 10514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10514
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.078"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            <1..629
                     /gene="capA"
                     /locus_tag="NBI57_001487"
     CDS             <1..629
                     /gene="capA"
                     /locus_tag="NBI57_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="capsular polysaccharide type 5/8 biosynthesis
                     protein CapA"
                     /protein_id="HCD1822429.1"
                     /translation="QKNLKILIILPLLFLIISAIVTFFVLSPKYQANTQILVNQTKGD
                     NPQFMAQEVQSNIQLVNTYKEIVKSPRILDEVSKDLNDKYSPSKLSSMLTITNQENTQ
                     LINIQVKSGHKQDSEKIANSFAKVTSKQIPKIMSVDNVSILSKADGTAVKVAPKTVVN
                     LIGAFFLGLVVALIYIFFKVIFDKRIKDEEDVEKELGLPVLGSIQKFN"
     gene            645..1331
                     /gene="cap8B"
                     /locus_tag="NBI57_001488"
     CDS             645..1331
                     /gene="cap8B"
                     /locus_tag="NBI57_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 8 capsular polysaccharide synthesis protein
                     Cap8B"
                     /protein_id="HCD1822430.1"
                     /translation="MSKKENTTTTLFVYEKPKSTISEKFRGIRSNIMFSKANGEVKRL
                     LVTSEKPGAGKSTVVSNVAITYAQAGYKTLVIDGDMRKPTQNYIFNEQNNNGLSSLII
                     GRTTMSEAITSTEIENLDLLTAGPVPPNPSELIGSERFKELVDLFNKRYDIIIVDTPP
                     VNTVTDAQLYARAIKDSLLVIDSEKNDKNEVKKAKALMEKAGSNILGVILNKTKVDKS
                     SSYYHYYGDE"
     gene            1334..2098
                     /gene="cap8C"
                     /locus_tag="NBI57_001489"
     CDS             1334..2098
                     /gene="cap8C"
                     /locus_tag="NBI57_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 8 capsular polysaccharide synthesis protein
                     Cap8C"
                     /protein_id="HCD1822431.1"
                     /translation="MIDIHNHILPNIDDGPTNETEMMDLLKQATTQGVTEIIVTSHHL
                     HPRYTTPIEKVKSCLNHIESLEEVQALNLKFYYGQEIRITDQILNDIDRKVITGINDS
                     RYLLIEFPSNEVPHYTDQLFFELQSKGFVPIIAHPERNKAISQNLDILYDLINKGALS
                     QVTTASLAGISGKKIRKLAIQMIENNLTHFIGSDAHNTEIRPFLMKDLFNDKKLRDYY
                     EDMNGFISNAKLVVDDKKIPKRMPQQDYKQKRWFGL"
     gene            2118..3941
                     /gene="cap8D"
                     /locus_tag="NBI57_001490"
     CDS             2118..3941
                     /gene="cap8D"
                     /locus_tag="NBI57_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 8 capsular polysaccharide synthesis protein
                     Cap8D"
                     /protein_id="HCD1822432.1"
                     /translation="MAHLSVKLRLLILALIDSLIVTFSVFVSYYILEPYFKTYSVKLL
                     ILAAISLFISHHISAFIFNMYHRAWEYASVSELILIVKAVTTSIVITMVVVTIVTGNR
                     PFFRLYLITWMMHLILIGGSRLFWRIYRKYLGGKSFNKKPTLVVGAGQVGSMLIRQML
                     KSDEMKLEPVLAVDDDEHKRNITITEGVKVQGKIADIPELVRKYKIKKIIIAIPTIGQ
                     ERLKEINNICHMDGVELLKMPNIEDVMSGELEVNQLKKVEVEDLLGRDPVELDMDMIS
                     NELTNKTILVTGAGGSIGSEICRQVCNFYPERIILLGHGENSIYLINRELRNRFGKNV
                     DIVPIIADVQNRARMFEIMETYKPYAVYHAAAHKHVPLMEDNPEEAVHNNILGTKNTA
                     EAAKNAEVKKFVMISTDKAVNPPNVMGASKRIAEMIIQSLNDETHRTNFVAVRFGNVL
                     GSRGSVIPLFKSQIEEGGPVTVTHPEMTRYFMTIPEASRLVLQAGALAEGGEVFVLDM
                     GEPVKIVDLARNLIKLSGKKEDDIRITYTGIRPGEKMFEELMNKDEVHPEQVFEKIYR
                     GKVQHMKCNEVEAIIQDIVNDFSKEKIINYANGKKGDNYVR"
     gene            3931..4959
                     /gene="cap8E"
                     /locus_tag="NBI57_001491"
     CDS             3931..4959
                     /gene="cap8E"
                     /locus_tag="NBI57_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 8 capsular polysaccharide synthesis protein
                     Cap8E"
                     /protein_id="HCD1822433.1"
                     /translation="MFDDKILLITGGTGSFGNAVMKRFLDSNIKEIRIFSRDEKKQDD
                     IRKKYNNSKLKFYIGDVRDSQSVETAMRDVDYVFHAAALKQVPSCEFFPVEAVKTNII
                     GTENVLQSAIHQNVKKVICLSTDKAAYPINAMGISKAMMEKVFVAKSRNIRSEQTLIC
                     GTRYGNVMASRGSVIPLFIDKIKAGEPLTITDPDMTRFLMSLEDAVELVVHAFKHAET
                     GDIMVQKAPSSTVGDLATALLELFEADNAIEIIGTRHGEKKAETLLTREEYAQCEDMG
                     DYFRVPADSRDLNYSNYVETGNEKITQSYEYNSDNTHILTVEEIKEKLLTLEYVRNEL
                     NDYKASMR"
     gene            4972..6081
                     /gene="cap8F"
                     /locus_tag="NBI57_001492"
     CDS             4972..6081
                     /gene="cap8F"
                     /locus_tag="NBI57_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 8 capsular polysaccharide synthesis protein
                     Cap8F"
                     /protein_id="HCD1822434.1"
                     /translation="MNIVITGAKGFVGKNLKADLTSTTDHHIFEVHRQTKEEELESAL
                     LKADFIVHLAGVNRPEHDKEFSLGNVSYLDHVLDILTRNTKKPAILLSSSIQATQDNP
                     YGESKLQGEQLLREYAEEYGNTVYIYRWPNLFGKWCKPNYNSVIATFCYKIARNEEIQ
                     VNDRNVELTLNYVDDIVAEIKRAIEGTPTIENGVPTVPNVFKVTLGEIVDLLYKFKQS
                     RLDRTLPKLDNLFEKDLYSTYLSYLPSTDFSYPLLMNVDDRGSFTEFIKTPDRGQVSV
                     NISKPGITKGNHWHHTKNEKFLVVSGKGVIRFRHVNDDEIIEYYVSGDKLEVVDIPVG
                     YTHNIENLGDTDMVTIMWVNEMFDPNQPDTYFLEV"
     gene            6085..7209
                     /gene="cap8G"
                     /locus_tag="NBI57_001493"
     CDS             6085..7209
                     /gene="cap8G"
                     /locus_tag="NBI57_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 8 capsular polysaccharide synthesis protein
                     Cap8G"
                     /protein_id="HCD1822435.1"
                     /translation="MEKLKLMTIVGTRPEIIRLSSTIKACDQYFNQILVHTGQNYDYT
                     LNQIFFDDLELRQPDHYLEAVGSNLGETMGNIIAKTYDVLLREQPDALLILGDTNSCL
                     AAVSAKRLKIPVFHMEAGNRCFDQNVPEEINRKIVDHVSDVNLPYTEHSRRYLLDEGF
                     NKANIFVTGSPMTEVIEAHRDKINHSDVLNKLGLEPQQYILVSAHREENIDNEKNFKS
                     LMNAINDIAKKYKMPVIYSTHPRSWKKIEESKFEFDPLVKQLKPFGFFDYNALQKDAF
                     VVLSDSGTLSEESSILKFPGVLIRTSTERPEVLDKGTVIVGGITYNNLIQSVELAREM
                     QNNNEPMIDAIDYKDTNVSTKVVKIIQSYKDIINRNTWRK"
     gene            7212..7838
                     /locus_tag="NBI57_001494"
     CDS             7212..7838
                     /locus_tag="NBI57_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic acetyltransferase"
                     /protein_id="HCD1822436.1"
                     /translation="MRIAIEKIIGLLKNQSSKESNVKIHRLAYITNSKFDGNNYIDRW
                     CKIRNSHIGEYSYIGFGSDFNNVEVGRYCSISSDVKIGLGKHPTHFFSSSPIFYSNNN
                     PFNIKQKFIDFNDQPSRTTIKNDVWIGANVIIMDGLTINTGAVIAAGSVVTKNVGAYE
                     VVGGVPAKVIKKRFDNKTIEKLLESKWWEKTPDKLKGFSVEYLNKKDT"
     gene            7843..8952
                     /locus_tag="NBI57_001495"
     CDS             7843..8952
                     /locus_tag="NBI57_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HCD1822437.1"
                     /translation="MRILNIVSSNIVQDPRVLKQIETIKGVTNDYKIVGMNNSQATNR
                     RLENLDCNYRLLGSKVDPKNILSKLIKRIRFATGVIREIKAFKPDVIHANDFDVLLMV
                     YLSNYKKANIVYDAHEIYAKNAFINKVPLISKFVESIEKHIVKHRVNAFVTVSHAAKE
                     YYQSKGYKKEANVITNAPILNDSREFKEIENFKEIVYQGQIVMDRGYEEFIIASSAFK
                     QNAPSFIIRGFGPHEEVIKELISYNSENIRLDKPVEVKELVDKLAESNVGVILTKPVS
                     INFEYTVSNKIFECIHAGLPVILSPVKEHIYLNEKYKFGIVLKEVTPLEIEKAVRKLR
                     DNHDLFNHLRQNAIKASKILNWQIESERLVELYKF"
     gene            8966..10132
                     /locus_tag="NBI57_001496"
     CDS             8966..10132
                     /locus_tag="NBI57_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HCD1822438.1"
                     /translation="MKFFVLCAIISMNIFIVISTFTKEVLGFPIEPVYYSTMVGIALI
                     TTVFAIYKIIVTQEIPRGLILLIAICLLYLAFYYFSPDKEEKLAKNNILFFLTWAVPA
                     AISGIYIKYINKATVERFFKLVFFIFSISFIFVILIPKLTGEIPSYINFGLMNYQNAS
                     YLSAFTAGLGIYFIMKGSVKHKWIYVLFTIIDIPIVFIPGGRGGAILLILYGLFAFIL
                     ITFKRGIPIAVKSIMYIFALSISSVLIYFLFTKGSNTRTFSYLQGGTLNLEGTSGRGP
                     IYEKGIYFIQQSPLLGYGPFNYYKLIGNIPHNIIIELILSFGLLGFFIIMICILLLVY
                     KMIRNYDPNTIDLLVMFIAIYPITLLMFSSNYLVVSEFWFVLFYFITKGRRHHG"
     gene            10125..>10514
                     /locus_tag="NBI57_001497"
     CDS             10125..>10514
                     /locus_tag="NBI57_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular biosynthesis protein"
                     /protein_id="HCD1822439.1"
                     /translation="MVKKVFIMDSVKTIIGTLLIALGLQFLAYPIINQRVGNEAFGSI
                     LTIYTIITITSVVLGNTLNNIRLINMNLYKSNHYYWKFVSILLISILIESIALIIVFL
                     YFFNLNTIDIIFLILLNILMCLRIYLNV"
BASE COUNT         3803 a         1428 c         1986 g         3297 t
ORIGIN      
        1 tacaaaaaaa cttgaagatt ttaattattt taccgctatt atttttaatt attagcgcta
       61 ttgttacatt tttcgtctta tcacctaaat atcaagctaa tactcaaatc ttagtgaatc
      121 aaactaaggg tgacaatcct cagtttatgg cacaagaggt tcaaagtaat attcaacttg
      181 taaatacgta taaagaaatt gttaaaagtc ctagaatttt agatgaggtg tcaaaggact
      241 taaatgataa gtattcacca tctaaattgt cgagtatgtt gacaattaca aaccaagaaa
      301 atacgcaact tatcaacatc caagttaaaa gtggtcataa acaagattcg gaaaaaattg
      361 cgaatagctt cgctaaagtt acaagtaaac aaattccgaa gattatgagt gtggataacg
      421 tatcaatttt atctaaagca gacggtacag cagttaaagt cgcaccaaaa actgtagtga
      481 atctaatcgg tgcattcttt ttaggattag ttgtcgcgct tatatatatc ttcttcaaag
      541 taattttcga taagcgaatt aaagatgaag aagatgtaga gaaagaatta ggattgcctg
      601 tattgggttc aattcaaaaa tttaattaag gatggttgct acttatgtca aaaaaggaaa
      661 atacgacaac aacactattt gtatatgaaa aaccaaaatc aacaattagt gaaaagtttc
      721 gaggtatacg ttcaaacatc atgttttcaa aagcaaatgg tgaagtaaag cgcttattgg
      781 ttacttctga aaagcctggt gcaggtaaaa gtacagttgt atcgaatgta gcgattactt
      841 atgcacaagc aggctataag acattagtta ttgatggcga tatgcgtaag ccaacacaaa
      901 actatatttt taatgagcaa aataataatg gactatcaag cttaatcatt ggtcgaacga
      961 ctatgtcaga agcaattacg tcgacagaaa ttgaaaattt agatttgcta acagctggcc
     1021 ctgtacctcc aaatccatct gagttaattg ggtctgaaag gttcaaagaa ttagttgatc
     1081 tgtttaataa acgttacgac attattattg tcgatacacc gccagttaat actgtgactg
     1141 atgcacaact atatgcgcgt gctattaaag atagtctgtt agtaattgat agtgaaaaaa
     1201 atgataaaaa tgaagttaaa aaagcaaaag cacttatgga aaaagcaggc agtaacattc
     1261 taggtgtcat tttgaacaag acaaaggtcg ataaatcttc tagttattat cactattatg
     1321 gagatgaata agtatgattg atattcataa ccatatattg cctaatatcg atgacggtcc
     1381 gacaaatgaa acagagatga tggatctttt aaaacaagcg acaacacaag gtgttacaga
     1441 aatcattgta acatcacatc acttacatcc tcgatatacc acacctatag aaaaagtgaa
     1501 atcatgttta aaccatattg aaagcttaga ggaagtacaa gcactaaatc taaagtttta
     1561 ttatggtcag gaaataagaa ttaccgatca aatccttaat gatattgatc gaaaagttat
     1621 taccggtatt aatgattcac gctatttact aatagaattt ccatcaaatg aagttccaca
     1681 ctatactgat caattatttt tcgaattaca gagtaaaggc tttgtaccga ttattgcaca
     1741 tccagagcgg aataaagcaa taagtcaaaa ccttgacata ctatacgatt taattaacaa
     1801 aggtgcttta agtcaagtga caacggcgtc attagcgggt atttccggta aaaaaattag
     1861 aaaattagca attcaaatga ttgaaaacaa tctgacacat ttcatcggtt cagatgcgca
     1921 taacacagaa atcagaccgt tcttaatgaa agacttattt aatgataaga aattacgtga
     1981 ttattatgaa gatatgaacg gatttattag taatgcgaag ttagttgttg atgataaaaa
     2041 aattcctaaa cgaatgccac aacaagatta taaacagaaa agatggtttg ggttataaac
     2101 agcaaatgag gggttttatg gcacatttat ctgtgaaatt gcggctttta atactagcat
     2161 taatcgattc actgatagtg acattttcag tattcgtaag ttattacatt ttagaaccgt
     2221 atttcaaaac atattctgtc aaattattaa tattggcagc tatatcacta ttcatatcgc
     2281 atcatatttc agcatttatt tttaatatgt atcatcgagc gtgggaatat gccagtgtga
     2341 gtgaattgat tttaattgtt aaagctgtga cgacatctat cgttattacg atggtggtcg
     2401 tgacaattgt tacaggcaat agaccgtttt ttagattgta tttaattact tggatgatgc
     2461 acttgatttt aataggtggc tcaaggttat tttggcgtat ttatcggaaa taccttggag
     2521 gtaagtcatt taataagaag ccaactttag ttgttggtgc tggtcaagta ggttcaatgc
     2581 tgattagaca aatgttgaaa agtgacgaaa tgaaacttga accggtatta gcagtcgatg
     2641 atgacgaaca taaacgcaat atcacaatta ctgagggtgt aaaagtccaa ggtaaaattg
     2701 cggatattcc agaactagtg aggaaatata agattaaaaa aatcatcatt gcaattccaa
     2761 ctattggtca agagcgtttg aaagaaatta ataatatttg ccatatggat ggcgttgagt
     2821 tattgaaaat gccaaatata gaagacgtca tgtctggtga gttagaagtg aatcaactga
     2881 aaaaagttga agtagaagat ttactaggca gagatcctgt tgaattagat atggatatga
     2941 tatcaaatga attgacgaat aaaactattt tagttacggg tgcaggtggt tcaataggat
     3001 cagaaatttg tagacaagtt tgtaatttct atccagaacg tattattcta cttggccatg
     3061 gtgaaaacag tatttattta atcaatcgtg aattgcgaaa tcgcttcgga aaaaatgttg
     3121 atatcgttcc tattatagcg gatgtgcaaa atagagcgcg tatgtttgaa attatggaaa
     3181 cgtataaacc atacgcagtt tatcatgcag cagcacacaa gcacgtgccg ttaatggaag
     3241 acaaccctga agaagcagta cataataata ttttaggtac gaaaaatact gctgaagctg
     3301 ctaaaaatgc agaggtaaag aaattcgtta tgatttctac ggataaagcc gttaatccgc
     3361 ctaatgtcat gggagcttca aagcgaattg cagaaatgat tattcaaagt ttaaatgatg
     3421 aaacgcatcg aacaaatttt gttgcagtga gatttggtaa tgtacttgga tcgagaggat
     3481 ctgtgattcc acttttcaaa agtcaaattg aagaaggtgg gccagttact gtgacacatc
     3541 ctgaaatgac acgttacttt atgacaattc ctgaagcttc tagactagtt ttgcaggcag
     3601 gggcattagc agaaggtggc gaagtatttg tgctagatat gggagaacca gtgaaaattg
     3661 tagatttggc acgtaattta attaagctaa gtggtaaaaa agaagacgac atacgcatta
     3721 cttatacagg gattagaccc ggcgaaaaaa tgtttgaaga gcttatgaat aaagatgagg
     3781 ttcatcctga acaagtattt gaaaaaattt atcgtggcaa agtacaacat atgaaatgta
     3841 atgaagttga agcgattatt caagacatcg tcaatgactt tagtaaagaa aaaattatta
     3901 actatgccaa tggcaaaaag ggagataatt atgttcgatg acaaaatttt attaattact
     3961 gggggcacag gatcattcgg taatgctgtt atgaaacggt ttttagattc taatattaaa
     4021 gaaattcgta ttttttcacg cgatgagaaa aaacaagatg acattcgaaa aaaatataat
     4081 aattcaaaat taaagttcta cattggtgat gtgcgtgata gtcaaagtgt agaaacagca
     4141 atgcgagatg ttgattacgt attccatgca gcagctttaa aacaagtgcc gtcatgtgaa
     4201 ttctttccag ttgaggcagt gaagacaaat attattggta cagaaaatgt cttacaaagt
     4261 gctattcatc aaaatgttaa aaaagtcata tgtttatcta cagataaggc agcgtatcct
     4321 attaatgcta tgggtatttc aaaagcaatg atggaaaaag tattcgtagc caaatcaaga
     4381 aatattcgta gtgaacaaac gcttatttgt ggtacaagat acggtaatgt gatggcttca
     4441 agaggatcag taataccttt gtttatcgac aaaatcaaag ctggagaacc tttaacgatt
     4501 acagatcctg atatgacaag atttttaatg agcttagaag atgcggtaga actagttgtt
     4561 catgcattta agcatgcaga gacaggagat attatggttc aaaaagcacc aagctcaacg
     4621 gtaggggatc ttgcgaccgc attattagaa ttgtttgaag ctgataatgc aattgaaatc
     4681 attggtacgc gacatggaga gaaaaaagca gaaacattgt tgacgagaga agaatacgca
     4741 caatgtgaag atatgggtga ttattttaga gtgccggcag actccagaga tttaaattat
     4801 agtaattatg ttgaaaccgg taacgaaaag attacgcaat cttatgaata taactccgat
     4861 aatacacata ttttaacggt ggaagagata aaagaaaaac ttttaacact agaatatgtt
     4921 agaaacgaat tgaatgatta taaagcttca atgagatagg agagattgac gttgaatatt
     4981 gtaattacag gagcaaaagg ttttgtagga aaaaacttga aagcagattt aacatcaacg
     5041 acagatcatc atattttcga agtacatcga caaactaaag aggaagaatt agagtcagca
     5101 ttgttgaaag cagactttat cgtgcattta gcgggtgtta atcgacctga acatgacaaa
     5161 gaattcagct taggaaacgt gagttattta gatcatgtac ttgatatatt aactagaaat
     5221 acgaaaaagc cagcgatatt attatcgtct tcaatacaag caacacaaga taatccttat
     5281 ggtgagagta agttgcaagg ggaacagcta ttaagagagt atgccgaaga gtatggcaat
     5341 acggtttata tttatcgctg gccaaattta ttcggcaagt ggtgtaagcc gaattataac
     5401 tcagtgatag caacattttg ttacaaaatt gcacgtaacg aagagattca agttaatgat
     5461 cggaatgttg aactaacgct aaactacgtg gatgatatcg tcgctgaaat aaagcgtgct
     5521 attgaaggaa ctccaacgat tgaaaatggt gtacctacag taccaaacgt atttaaagtg
     5581 acattgggag aaattgtaga tttattatac aagttcaaac agtcacgtct cgatcgaaca
     5641 ttgccgaaat tagataactt gtttgaaaaa gatttgtata gtacgtattt aagctatcta
     5701 cctagtacag actttagtta tcccttactt atgaatgtgg atgatagggg ttcttttaca
     5761 gaatttataa aaacaccgga tcgtggtcaa gtttctgtaa atatttctaa accaggtatt
     5821 actaaaggta atcactggca ccatactaaa aacgaaaaat ttctagtcgt atcaggtaaa
     5881 ggggtaattc gttttagaca tgttaatgat gatgaaatca ttgaatatta cgtttctggc
     5941 gataaattag aagttgtaga cataccagta ggatacacac ataatattga aaatttaggc
     6001 gacacagata tggtaactat tatgtgggtg aatgaaatgt ttgatccaaa tcagccagat
     6061 acgtatttct tggaggtata gcgcatggaa aaactgaaat taatgacaat agttggtaca
     6121 aggcctgaaa tcattcgttt atcatcaacg attaaagcat gtgatcaata ttttaatcag
     6181 atattagtac acactggtca aaattatgat tatacattga atcaaatttt ctttgatgat
     6241 ttggaattaa gacaaccgga ccactactta gaggcagttg gaagtaacct tggagaaacg
     6301 atggggaata ttattgcgaa gacatatgac gttttattac gcgaacaacc agatgcactt
     6361 ttaattcttg gtgatacaaa tagttgttta gcagcagtat ctgctaaacg attaaagatt
     6421 cctgtgttcc acatggaagc gggtaataga tgctttgatc agaatgtacc tgaagaaatc
     6481 aatcgtaaaa ttgttgacca tgtcagtgat gtgaatctac cttatacaga acatagcaga
     6541 cggtatttat tagatgaagg cttcaataaa gcgaatatct ttgtgacagg atcaccgatg
     6601 acagaagtga tagaagcgca tcgagataaa attaatcaca gtgacgtttt aaataaacta
     6661 ggattagaac cgcaacaata cattttagta tctgcgcata gagaagagaa tatcgataat
     6721 gaaaagaatt ttaaatcatt aatgaatgcg ataaatgata ttgccaaaaa gtataaaatg
     6781 cctgtgattt attcaacgca tccaagaagt tggaagaaaa ttgaagaaag taaatttgaa
     6841 tttgatccat tagttaaaca gttaaagcca tttggtttct ttgattataa tgcattgcaa
     6901 aaagatgcat ttgttgtgct atcagatagt ggaacattgt cagaagagtc gtctattttg
     6961 aagttccctg gtgtccttat tcgaacttcc acagaaagac cggaagtact agataaaggt
     7021 acggttattg taggtggtat tacctataac aatctaatcc aatccgttga actagcaaga
     7081 gagatgcaaa acaataacga accgatgatt gatgctattg attataaaga cactaacgtt
     7141 tcgacaaagg tagttaaaat tattcaaagc tataaagata ttatcaatcg aaatacttgg
     7201 aggaaatgac gatgaggata gcgattgaaa agataattgg tttgctgaaa aaccagtcct
     7261 ctaaagaatc gaatgttaag attcatcgct tggcgtatat tacaaactca aaatttgatg
     7321 gcaataacta tatagataga tggtgtaaaa tcaggaattc tcacattggt gaatacagtt
     7381 atattggatt tggtagtgat tttaataatg tagaagtagg aagatattgt tcgatatctt
     7441 cggatgtaaa aattgggtta ggaaaacatc ctacacactt ttttagctca tcaccgattt
     7501 tttattctaa taataatcca tttaacataa agcaaaagtt tatagacttt aatgaccaac
     7561 caagccgtac aacaattaaa aatgatgtgt ggattggtgc aaatgtaatt attatggatg
     7621 gattaacaat aaatactggt gcagtcatag cagccggctc agttgttact aaaaatgtag
     7681 gagcatatga ggttgttggt ggggttcctg caaaagtgat taagaagcga tttgacaata
     7741 aaacaattga aaaacttttg gaaagcaagt ggtgggagaa aacgcctgac aaactaaaag
     7801 gattttcggt tgaatattta aataaaaagg atacttaatg atatgagaat tttaaatatt
     7861 gtatcgagta atattgttca agacccaagg gtacttaaac aaatagaaac aattaaaggc
     7921 gttacgaatg attataaaat tgttggaatg aataattcac aagctactaa taggcgattg
     7981 gaaaatttag attgtaatta tcgtttgtta ggtagcaagg tagatcccaa aaatattctt
     8041 tctaaattaa ttaagcgtat aagatttgca acaggtgtta tccgagaaat taaagctttt
     8101 aaacctgacg tgattcatgc aaatgatttc gacgtattat taatggtcta tttaagcaat
     8161 tataaaaaag ctaatattgt ttatgatgcg catgaaatat atgcgaaaaa tgcctttatt
     8221 aataaagttc cacttatttc aaagtttgta gaaagtatag aaaaacacat agtaaaacat
     8281 cgtgttaatg ccttcgtaac agtaagtcat gcagcaaaag aatattatca atctaaagga
     8341 tataagaagg aagcgaatgt tattacgaat gcacctattt taaatgatag cagagaattt
     8401 aaagaaatcg aaaactttaa agaaatcgta tatcaaggtc aaattgtaat ggacagagga
     8461 tatgaagagt ttattattgc ttcatcagct tttaaacaaa atgctccttc attcataatt
     8521 cgagggtttg gtccgcatga agaagtgata aaagaactga ttagttataa ctcggaaaat
     8581 attaggttgg ataaaccagt tgaagtaaaa gaattggttg ataagttagc agaaagtaat
     8641 gttggtgtta tcttgacgaa acctgtatct attaattttg aatatacagt atctaataaa
     8701 atttttgaat gtatacatgc tggtttacca gtaattttat ctcctgtcaa agagcatatt
     8761 tatctcaatg aaaaatataa atttggcatt gttttaaagg aagttacgcc gttagaaatt
     8821 gaaaaggcgg ttagaaaatt aagagataat cacgatttgt ttaatcattt acgtcaaaat
     8881 gcaattaagg cgtctaaaat tttgaattgg caaatagaaa gtgaacgatt agtagaatta
     8941 tataaatttt aaagagaggt aaactatgaa attttttgta ctttgtgcaa ttatcagcat
     9001 gaacatattt atagtaatct ctacatttac taaagaagta ttagggttcc ctatagagcc
     9061 ggtgtattac tcaaccatgg ttggtatagc attaattact acggtgtttg ctatttataa
     9121 gataattgtc acgcaagaaa ttccgcgagg gttaatatta ttaattgcta tatgtttgct
     9181 ttatctagct ttttattatt tttcaccaga taaggaagag aaactagcta aaaataatat
     9241 tctattcttt ttaacatggg cagttccagc ggcaattagt ggtatttata ttaaatatat
     9301 aaacaaggct acggtagaaa gattttttaa attagtattt ttcatatttt ctatttcatt
     9361 tatttttgta attttaatac caaaacttac aggtgagata cctagctata tcaattttgg
     9421 acttatgaac tatcaaaacg cttcgtacct ttcagcattt actgccggat taggcattta
     9481 tttcattatg aaaggttcag tgaaacataa gtggatatat gttctattta caataattga
     9541 tatccctatt gtgtttatac caggagggcg tggaggtgct attttattaa ttctttacgg
     9601 cttatttgca tttatactta ttacgtttaa aagaggaata cctattgcag taaaaagcat
     9661 tatgtatatt tttgcattaa gcatatctag tgtattgatt tactttcttt ttacaaaagg
     9721 ttcgaatact agaacatttt catatctaca aggtggaaca cttaatttag aaggtacttc
     9781 tggaagagga ccgatttatg aaaaaggtat ttactttatt caacaaagtc cgttattagg
     9841 ctatgggcca tttaactatt ataaactaat cggaaatata ccacataaca tcattattga
     9901 gttgattcta tcatttggct tattagggtt ttttatcata atgatttgca ttttgctact
     9961 agtttataaa atgattagga actatgatcc aaacactata gatttactcg ttatgtttat
    10021 agcaatctat ccaatcacat tattaatgtt tagttcaaat tatttagttg taagtgaatt
    10081 ttggtttgtg ttgttctatt ttattacaaa aggacggcgt catcatggtt aagaaagttt
    10141 ttattatgga tagcgtaaag acaataattg gtacgttgct tatagcttta ggattacaat
    10201 ttttagctta tccaattatt aatcaacgag taggtaatga agcgtttggt tctattttaa
    10261 cgatttatac aataataaca atcacgagtg ttgtattagg caatacgctt aacaatatac
    10321 gattaattaa tatgaatcta tacaaatcca atcattacta ctggaaattt gtgtcgatac
    10381 ttttaatttc aattctgatt gagagtatag ctttaattat tgtatttctt tactttttta
    10441 atttgaacac catcgatatt atctttttaa ttctacttaa tattttaatg tgtttaagga
    10501 tttatctgaa tgta
//
LOCUS       DAHHSC010000079        10393 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.079, whole genome shotgun sequence.
ACCESSION   DAHHSC010000079 DAHHSC010000000
VERSION     DAHHSC010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 10393)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10393)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10393
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.079"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            <1..270
                     /locus_tag="NBI57_001498"
     CDS             <1..270
                     /locus_tag="NBI57_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular biosynthesis protein"
                     /protein_id="HCD1822440.1"
                     /translation="FIILGNIGVLFNAVSIMIQTLNTKHASITLQANYMTLHTITFIF
                     ITILMTIAFGLNGFFWTTLFSNIIKYVILNIIGLKSKFINKKDVD"
     gene            271..1476
                     /gene="cap8L"
                     /locus_tag="NBI57_001499"
     CDS             271..1476
                     /gene="cap8L"
                     /locus_tag="NBI57_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 8 capsular polysaccharide synthesis protein
                     Cap8L"
                     /protein_id="HCD1822441.1"
                     /translation="MSEKKILILCQYFYPEYVSSATLPTQLAEDLIANHINVDVMCGW
                     PYEYSNHKQVSKTEMHRGIRIRRLKYSRFNNKSKVGRIINFFSLFSKFVINIPKMLKY
                     DQILVYSNPPILPLIPDVLHRLLKKKYSFVVYDIAPDNAIKTGATRPGSMIDKLMRYI
                     NRHVYKNAENVIVLGTEMKNYLLNHQISKNADNIHVIPNWYDMRQLQDNRIYNDTFKA
                     YREQYDKILLYSGNMGQLQDMETLISFLKLNKDQPQTLTILCGHGKKFADVKTAIEDH
                     RIENVKMFEFLTGTDYADVLKIADVCIASLIKEGVGLGVPSKNYGYLAAKKPLVLIMD
                     KQSDIVQHVEQYDAGIQIDNGDAHAIYNFINTHSSKELHEMGERAHQLFKDKYTREIN
                     TMKYYNLLK"
     gene            1487..2044
                     /gene="cap8M"
                     /locus_tag="NBI57_001500"
     CDS             1487..2044
                     /gene="cap8M"
                     /locus_tag="NBI57_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 8 capsular polysaccharide synthesis protein
                     Cap8M"
                     /protein_id="HCD1822442.1"
                     /translation="MKRLFDVVSSIYGLVVLSPILLITALLIKMESPGPAIFKQKRPT
                     INNELFNIYKFRSMKIDTPNVATDLMDSTSYITKTGKVIRKTSIDELPQLLNVLKGEM
                     SIVGPRPALYNQYELIEKRTKANVHTIRPGVTGLAQVMGRDDITDDQKVAYDHYYLTH
                     QSMMLDMYIIYKTIKNIVTSEGVHH"
     gene            2044..2931
                     /gene="capN"
                     /locus_tag="NBI57_001501"
     CDS             2044..2931
                     /gene="capN"
                     /locus_tag="NBI57_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide type 5/8 biosynthesis
                     epimerase CapN"
                     /protein_id="HCD1822443.1"
                     /translation="MRKNILITGVHGYIGNALKDKLIEQGHQVDQINVRNQLWKSTSF
                     KDYDVLIHTAALVHNNSPQARLSDYMQVNMLLTKQLAQKAKAEDVKQFIFMSTMAVYG
                     KEGQVGKSDQIDTQTPMNPTTNYGISKKFAEQALQELISDSFKVAIVRPPMIYGAHCP
                     GNFQRLMQLSKRLPIIPNINNQRSALYIKHLTAFIDQLISLEVTGVYHPQDSFYFDTS
                     SVMYEIRRQSHRKTVLINMPSVLNKYFNKLSVFRKLFGNLTYSNTLYENNNALEVIPG
                     KMSLVIADIMDETTTKDKA"
     gene            2985..4247
                     /gene="cap8O"
                     /locus_tag="NBI57_001502"
     CDS             2985..4247
                     /gene="cap8O"
                     /locus_tag="NBI57_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 8 capsular polysaccharide synthesis protein
                     Cap8O"
                     /protein_id="HCD1822444.1"
                     /translation="MKLTVVGLGYIGLPTSIMFAKHGVDVLGVDINQQTIDKLQSGQI
                     SIEEPGLQEVYEEVLSSGKLKVSTTPEASDVFIIAVPTPNNDDQYRSCDISLVMRALD
                     SILPFLEKGNTIIVESTIAPKTMDDFVKPVIENLGFTIGEDICLVHCPERVLPGKILE
                     ELVHNNRIIGGVTKACIEAGKYVYRTFVQGEMIETDARTAEMSKLMENTYRDVNIALA
                     NELTKICNNLNINVLDVIEMANKHPRVNIHQPGPGVGGHCLAVDPYFIIAKDPENAKL
                     IQTGREINNSMPAYVVDTTKQIIKALSGNKVTVFGLTYKGDVDDIRESPAFDIYELLN
                     QEPDIEVCAYDPHVELDFVEHDMSHAVKDASLVLILSDHSEFKNLSDSHFDKMKHKVI
                     FDTKNVVKSSFEDVSYYNYGNIFNFIDK"
     gene            4324..5469
                     /gene="cap8P"
                     /locus_tag="NBI57_001503"
     CDS             4324..5469
                     /gene="cap8P"
                     /locus_tag="NBI57_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 8 capsular polysaccharide synthesis protein
                     Cap8P"
                     /protein_id="HCD1822445.1"
                     /translation="MKKIMVIFGTRPEAIKMAPLVKEIDHNGNFEANIVITAQHRDML
                     DSVLSIFDIQADHDLNIMQDQQTLADLTANALAKLDSIINEEQPDMILVHGDTTTTFV
                     GSLAAFYHQIPVGHVEAGLRTHQKYSPFPEELNRVMVSNIAELNFAPTVIAAKNLLFE
                     NKDKERIFITGNTVIDALSTTVQNDFVSTIINKHKGKKVILLTAHRRENIGEPMHQIF
                     KAVRDLADEYKDVVFIYPMHRNPKVRAIAEKYLSGRNRIELIEPLDAIEFHNFTNQSY
                     LVLTDSGGIQEEAPTFGKPVLVLRNHTERPEGVEAGTSRVIGTDYDNIVRNVKQLIED
                     DEAYQRMSQANNPYGDGQASRRICEAIEYYFGLRSDKPDEFVPLRHK"
     gene            complement(5534..5860)
                     /gene="isdI"
                     /locus_tag="NBI57_001504"
     CDS             complement(5534..5860)
                     /gene="isdI"
                     /locus_tag="NBI57_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphylobilin-forming heme oxygenase IsdI"
                     /protein_id="HCD1822446.1"
                     /translation="MFMAENRLQLQKGSAEETIERFYNRQGIETIEGFQQMFVTKTLN
                     TEDTDEVKILTIWESEDSFNNWLNSDVFKEAHKNVRLKSDDDGQQSPILSNKVFKYDI
                     GYHYQK"
     gene            complement(5867..6250)
                     /locus_tag="NBI57_001505"
     CDS             complement(5867..6250)
                     /locus_tag="NBI57_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaN family protein"
                     /protein_id="HCD1822447.1"
                     /translation="MRLILIVIGLIFTALGIAGAVLPLLPTTPFLLVAVFCFARSSDR
                     FYNWLINQKIYKEYVENFYLHRGYTLQQKIKILISLYIVIGFSIYMVDVLAVRVGLII
                     MVIIQTAVLFTFVKTLPKSNHKIEE"
     gene            6677..8164
                     /locus_tag="NBI57_001506"
     CDS             6677..8164
                     /locus_tag="NBI57_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HCD1822448.1"
                     /translation="MAVNVRDYIAENYGLFINGEFVKGSSDETIEVTNPATGETLSHI
                     TRAKDKDVDHAVKVAQEAFESWSLTSKSERAQMLRDIGDKLMAQKDKIAMIETLNNGK
                     PIRETTAIDIPFAARHFHYFASVIETEEGTVNDIDKDTMSIVRHEPIGVVGAVVAWNF
                     PMLLAAWKIAPAIAAGNTIVIQPSSSTPLSLLEVAKIFQEVLPKGVVNILTGKGSESG
                     NAIFNHDGVDKLSFTGSTDVGYQVAEAAAKHLVPATLELGGKSANIILDDANLDLAVE
                     GIQLGILFNQGEVCSAGSRLLVHEKIYDQLVPRLQEAFSNIKVGDPQDEATQMGSQTG
                     KDQLDKIQSYIDAAKESDAQILAGGHRLTENGLDKGFFFEPTLIAVPDNHHKLAQEEI
                     FGPVLTVIKVKDDQEAIDIANDSEYGLAGGVFSQNITRALNIAKAVRTGRIWINTYNQ
                     VPEGAPFGGYKKSGIGRETYKGALSNYQQVKNIYIDTSNALKGLY"
     gene            8811..9770
                     /locus_tag="NBI57_001507"
     CDS             8811..9770
                     /locus_tag="NBI57_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HCD1822449.1"
                     /translation="MNGKKANTINRYKYFHHVNHQKIQQSSKKTLWASLIITLLFTVI
                     EFVGGLVSNSLALLSDSFHMLSDVLALGLSMLAIYFASKKPTARYTFGYLRFEILVAF
                     LNGLALIVISIWILYEAIVRIIYPQPIESGIMFMIASIGLLVNIILTIILVRSLKQED
                     NINIQSALWHFMGDLLNSIGVIVAVVLIYFTGWRIIDPIISIVISLIILRGGYKITRN
                     AWLILMESVPQHLDTDQIMADIKNIDGILDVHEFHLWSITTEHYSLSAHVVLDKKYEG
                     DDYQAIDQVSSLLKEKYGIAHSTLQIENLQLNPLDEPYFDKLT"
     gene            complement(10123..10326)
                     /locus_tag="NBI57_001508"
     CDS             complement(10123..10326)
                     /locus_tag="NBI57_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1822450.1"
                     /translation="MDKESVVASLARNKKIAVETMAGQRYIIERILHTNDEKHIHILK
                     PKDVVLDVDSIKEIDENHLNDAT"
BASE COUNT         3605 a         1526 c         1956 g         3306 t
ORIGIN      
        1 tttattattt taggtaatat aggtgtttta ttcaatgcag tgagtattat gatccaaact
       61 ttaaatacaa aacacgcatc aataacatta caagcgaatt atatgacgct tcacacgatt
      121 acatttatat tcataactat tttaatgaca attgcgtttg gtctaaatgg attcttttgg
      181 acaacgctgt tcagcaacat tattaagtat gtgattttaa atattatagg tttaaagtct
      241 aaattcatta ataaaaagga cgtcgattag atgagtgaaa aaaagatttt gattttatgt
      301 cagtattttt atccggaata tgtatcttct gcgacgttac caactcaatt ggcggaagat
      361 ttaattgcga atcacattaa tgtcgatgtc atgtgtggat ggccatatga atatagtaat
      421 cataaacagg tttctaaaac cgagatgcat cgtggaattc gcattcgacg tctcaagtat
      481 tcgaggttta ataacaaaag taaggttgga aggatcatca atttctttag tttattttca
      541 aaattcgtga ttaatatacc taaaatgttg aaatatgatc agattcttgt ttactctaat
      601 ccaccaatct tgccattaat accagacgtt ttacacagac tgcttaagaa aaaatattct
      661 tttgtggtgt atgatatagc acctgataat gcgattaaga caggtgcaac tcgtccaggt
      721 agcatgattg ataagctgat gcgttacatt aatagacatg tctacaagaa tgctgaaaat
      781 gtcattgtcc ttggtacgga aatgaaaaac tacttactaa atcatcaaat ttctaaaaat
      841 gctgacaata tccatgtgat tcctaactgg tatgacatgc gtcaattaca agacaatcgt
      901 atctataatg acacatttaa agcttaccgt gagcaatacg acaaaatttt attgtatagc
      961 ggtaatatgg ggcagttaca ggatatggag acacttatct catttttaaa attaaataag
     1021 gatcagcctc aaacgttaac aatactttgt ggtcatggta agaaatttgc agatgtcaaa
     1081 acggcaatag aagaccatcg tattgaaaat gttaaaatgt ttgagttttt aacaggtaca
     1141 gactatgctg acgtattaaa aattgcggat gtatgtattg catcgctgat taaagaaggc
     1201 gtcggtttag gcgttccgag caagaattat ggctaccttg cagctaagaa accgttggta
     1261 ctcatcatgg ataagcaatc tgatatcgtt caacatgttg aacaatatga tgcgggtatc
     1321 caaattgata atggcgatgc acatgccatt tataacttca tcaacactca ctcgagtaag
     1381 gaattgcacg agatgggtga gcgcgcacat caactgttta aagataaata tacgagagaa
     1441 attaatacta tgaagtatta caatctgttg aagtgaggag ataattatga agcgattatt
     1501 cgatgtagtg agttcaatat atggtttagt agttttaagt ccgattctgt taattacagc
     1561 attactaatt aaaatggaat cacctggacc agccattttc aaacaaaaaa gaccgacgat
     1621 taataatgaa ttgtttaata tttataagtt tagatcaatg aaaatagaca cacctaatgt
     1681 tgcaactgat ttaatggatt caacatcgta tataacaaag acagggaagg tcattcgtaa
     1741 gacctctatt gatgaattgc cacaattatt gaatgtttta aaaggagaaa tgtcaattgt
     1801 aggtcctaga ccagcgcttt ataatcaata cgaattaatc gaaaaacgta caaaagcgaa
     1861 cgtgcatacg attagaccag gtgtgacagg actagctcaa gtgatgggga gagatgatat
     1921 tactgatgat caaaaagtag cgtatgatca ttattactta acacatcaat ctatgatgct
     1981 tgatatgtat atcatatata aaacaattaa aaatatcgtt acttcagaag gtgtgcatca
     2041 ctaatgagaa aaaatatttt aattacaggc gtacatggat atatcggtaa tgctttaaaa
     2101 gataagctta ttgaacaagg acatcaagta gatcaaatta atgttaggaa tcaattatgg
     2161 aagtcgacct cgttcaaaga ttatgatgtt ttaattcata cagcagcttt ggttcacaac
     2221 aattcacctc aagcaaggct atctgattat atgcaagtga atatgttgct tacgaaacaa
     2281 ttggcacaaa aggctaaagc tgaagacgtt aaacaattta tttttatgag tactatggca
     2341 gtttatggaa aagaaggtca ggttggtaaa tcagatcaaa ttgatacaca aacaccaatg
     2401 aaccctacga ccaactatgg tatttccaaa aagttcgctg aacaagcatt acaagagttg
     2461 attagtgatt cgtttaaagt agcaattgtg agaccaccaa tgatttatgg tgcacattgc
     2521 ccaggaaatt tccaacggtt aatgcaattg tcaaagcgac tgccaatcat tcccaatatt
     2581 aacaatcagc gcagtgcatt atatattaaa catctgacag catttattga tcaattaata
     2641 tcattagaag tgacaggcgt gtatcatcct caagatagtt tttactttga tacatcgtca
     2701 gtaatgtatg aaatacgtcg ccaatcacat cgtaaaacgg tattgatcaa catgccttca
     2761 gtgttaaata agtattttaa taagttgtcg gtctttagaa aattattcgg caatttaaca
     2821 tacagcaata cgttatatga aaataataat gcacttgaag ttattcctgg aaaaatgtca
     2881 cttgttattg cggacatcat ggatgaaacg acaaccaaag ataaggcata agtcatctat
     2941 taaataaaat caacatacaa atcgttttat ttggaggtta tagtatgaag ttaacagtag
     3001 ttggcttagg ttatattggt ttaccaacat caattatgtt tgcaaagcat ggcgtcgatg
     3061 tgcttggtgt tgatattaat cagcaaacga ttgataagtt acaaagtggt caaattagta
     3121 ttgaagaacc tgggttacaa gaggtttatg aagaggtact gtcatcggga aaattgaagg
     3181 tatctacaac gccagaagca tctgatgttt ttatcattgc cgttccgacg ccgaataatg
     3241 atgatcagta ccggtcatgt gacatttcgc tagttatgcg tgcattagat agtattttac
     3301 catttttaga aaaagggaat actattattg tagagtcgac aattgcgcct aaaacgatgg
     3361 atgattttgt aaaaccagtc attgaaaatt taggatttac aataggtgaa gatatttgtt
     3421 tagtgcattg tccagaacgt gtactgccag gaaaaatttt agaagaatta gttcataaca
     3481 atcgtatcat tggcggtgtg actaaagctt gtattgaagc gggtaaatat gtctatcgca
     3541 cattcgttca gggagaaatg attgaaacag atgcacgtac tgctgaaatg agtaagctaa
     3601 tggaaaacac atatagagac gtgaatattg ctttagctaa tgaattaaca aaaatttgca
     3661 ataacttaaa tattaatgta ttagatgtga ttgaaatggc aaacaaacat ccgcgtgtta
     3721 atatccatca acctggtcca ggtgtaggcg gtcattgttt agctgttgat ccgtacttta
     3781 ttattgctaa agaccctgaa aatgcaaagt taattcaaac tggacgtgaa attaataatt
     3841 caatgccggc ctatgttgtt gatacaacga agcaaatcat caaagcgttg agcgggaata
     3901 aagtcacagt atttggttta acttataaag gtgatgttga tgatataaga gaatcgccag
     3961 catttgatat ttatgagcta ttaaatcaag aaccagacat agaagtatgt gcttatgatc
     4021 cacatgttga attagatttt gtggaacatg atatgtcaca tgctgtcaaa gacgcatcgc
     4081 tagtattgat tttaagtgac cactcagaat ttaaaaattt atcggacagt cattttgata
     4141 aaatgaagca taaagtgatt tttgatacaa aaaatgttgt gaaatcatca tttgaagatg
     4201 tatcgtatta taattatggc aatatattta attttatcga caaataaaat gtgtcaaact
     4261 agggcataca tgattaagga aagataagct gtcatgtgtt tgaacttcag agaggataat
     4321 gttatgaaaa aaattatggt tattttcggt acgagacccg aagcaataaa aatggcacca
     4381 ttagtaaaag aaattgatca taatgggaac tttgaagcga acattgtgat tacagcacaa
     4441 catagagata tgttagatag tgtgttaagt atatttgata ttcaagctga tcatgattta
     4501 aatattatgc aagatcaaca aacgttagcg gaccttacgg cgaatgcgct tgctaaactt
     4561 gatagcatca ttaatgagga acagccggat atgattttag tacatggtga tactacaacg
     4621 acttttgtag gaagtttggc agcattttat catcaaattc cggttggaca tgtagaagct
     4681 ggacttcgaa cacatcagaa atactcacca tttcctgaag agttaaatcg agtcatggta
     4741 agtaatattg ctgaattgaa ttttgcgcca acagtaattg cagctaaaaa tttacttttt
     4801 gaaaacaaag acaaagagcg tatctttatt actggaaata cagttattga cgcattgtca
     4861 acaacagttc aaaatgattt tgtttcaacg attattaata aacataaagg caagaaagtt
     4921 attttactaa cagcgcatcg tcgtgaaaat attggggaac cgatgcatca gatttttaaa
     4981 gcagtaagag atttggcaga tgaatataaa gatgttgtct tcatttatcc aatgcatcgt
     5041 aatccaaagg taagagcgat tgccgaaaaa tatttatctg ggagaaatcg gattgaatta
     5101 attgagccat tagatgcgat tgagttccat aattttacaa atcaatcgta cctcgtgctg
     5161 acagattctg gtggtattca agaggaggct cctacatttg gaaaacctgt gttggtatta
     5221 aggaatcata cagagcgtcc cgaaggcgtt gaggcgggaa catcgagagt aattggcaca
     5281 gattatgaca atattgttcg aaatgtgaaa caattgattg aggatgatga agcgtatcaa
     5341 cgtatgagtc aagcgaataa tccatatggt gatggacaag catcacgacg tatttgtgaa
     5401 gcaatagaat attattttgg attgcgctca gacaagccgg atgaattcgt acctttacgt
     5461 cacaaataat aaaaaacccc taatcatgaa gttggtttag acaaccagcg gtgactaggg
     5521 gtttttaata tatttatttt tgatagtggt agccaatatc atatttgaat actttatttg
     5581 ataatattgg actttgctgt ccatcgtcat cactttttaa acgtacattt ttatgagctt
     5641 ctttaaatac atcggaattc aaccaattat taaagctatc ttcagattcc caaatagtta
     5701 agattttaac ttcgtctgta tcctcggtat ttaatgtttt agtgacaaac atttgttgga
     5761 agccttcaat agtttcaatg ccttgtctat tgtaaaaacg ttcaatcgtt tcttccgcac
     5821 tgcctttttg taattgtaat ctattttctg ccataaacat gggcaatcac tcctctattt
     5881 tatgatttga tttgggtaat gtttttacaa atgtaaagag tacagcggtt tgtatgataa
     5941 ccattatgat taatcctaca cggactgcaa gaacatccac catataaatt gaaaaaccta
     6001 ttacaatgta taagctaatt aaaattttaa ttttctgttg tagcgtgtag cctcgatgta
     6061 aataaaagtt ttctacatat tctttataaa ttttttgatt aataagccaa ttgtaaaacc
     6121 gatctgaact tcgagcaaaa caaaaaactg ctacgagtaa aaaaggggtc gttggcagta
     6181 aaggtaatac ggcacctgca ataccaagcg ctgtaaatat taagccaata acgattaaaa
     6241 taagtcgcat tgaaaaaact ccattctagt actaatgcgc atgtaatatt gttttagtaa
     6301 tataactcat gctaaatata atgtgtatga taagtgcaat gactcagtaa aatgaaacgt
     6361 tgttgaatta ttcttgtccc attaacgcat tttaagcgcg actttcataa caaccaaact
     6421 atttaatgag aattattctc aagtattata gttatattat gtgttttatt tttgaaaagt
     6481 gcaatatgtt ttcgaaaata agattatttt tatgtgcaaa aacgacgcaa aagttttaaa
     6541 aatgagactt ctgtgagctg attattttat aaaatgtaaa cgcttactat ataatgtgaa
     6601 tcatatcgtt taaaagcatt attaaatatg atgctaagag atttatatta tagccaataa
     6661 acaaaggaga gataatatgg cagtaaacgt tcgagattat attgcagaga attatggttt
     6721 atttatcaat ggggaatttg ttaaaggtag cagtgacgaa acaatcgaag tgactaatcc
     6781 agcaactgga gaaacactat cacatattac aagagcaaaa gataaagatg tcgatcatgc
     6841 agtcaaagtg gcgcaagagg catttgaatc atggtcatta acttctaaat cagaacgtgc
     6901 acaaatgttg cgtgatattg gtgataaatt aatggcacaa aaagataaaa ttgcaatgat
     6961 tgaaacatta aataatggta aaccgattcg tgagacaaca gcaattgata ttccatttgc
     7021 tgcaagacat ttccattatt tcgcaagtgt tattgaaaca gaagaaggta cagtaaatga
     7081 tattgataaa gacacaatga gtatcgtacg acatgagccg attggcgtcg taggtgctgt
     7141 tgttgcttgg aacttcccaa tgctattagc tgcatggaag attgcgccag ccattgctgc
     7201 aggtaataca attgtgattc aaccttcgtc ttcaacacca ttaagtttat tggaagttgc
     7261 taaaattttc caagaggtat tacctaaagg tgttgtcaat atactaacgg gtaaaggttc
     7321 agaatcaggt aatgcaattt tcaatcatga tggtgtagat aaattatcat ttacgggctc
     7381 aactgatgta ggttatcaag ttgccgaagc tgcagcaaaa catctagtac ccgctacatt
     7441 agagcttggt ggtaaaagcg ccaatatcat attagatgat gctaatttag accttgcagt
     7501 tgaaggtatt cagttaggta ttttattcaa ccaaggtgaa gtatgtagtg caggttctcg
     7561 attattagtt catgaaaaaa tttatgatca attggtgcca cgtttacaag aggcattttc
     7621 aaatattaaa gttggagatc cacaagatga agctacacaa atgggtagtc aaactggtaa
     7681 ggatcaatta gataaaattc aatcatatat tgatgcagca aaagaatcag atgcacaaat
     7741 tttagcaggt ggtcatcgct taactgaaaa tggattagat aaagggttct tctttgagcc
     7801 gacattaatt gctgtgccag acaatcatca caaattagca caagaagaaa tatttggacc
     7861 agtgttaaca gtgattaaag tgaaggacga tcaagaagca attgatatag ctaatgattc
     7921 tgagtatggt ttagcaggcg gtgtattttc tcaaaatatc acacgtgcat taaatattgc
     7981 taaagctgta cgtacaggac gtatttggat taacacttac aaccaagtac cagaaggcgc
     8041 accatttggt ggttataaaa aatcaggtat cggtcgagaa acttataaag gtgcgttaag
     8101 taactatcaa caagttaaaa atatttatat tgatacaagc aatgctttaa aaggtttgta
     8161 ctagaataaa tatcgtttct gaagcgtgtt tgtaggtcag tctagcggta agtcttaaca
     8221 tttaacggcg ttgtttagat tttaagcaaa acaaaatata taggaacacg tatcatgata
     8281 ttaggatata atgactaaaa taatagcagt aggatggttt ttaattgcaa atcatcttac
     8341 tgctgttttt aattatgcta atttgcgatg cagctattat aaggacagag ttgtttatga
     8401 attgtggtga tttagaaata tgaagttcaa tatgcaaagt catcgtttgt tttaatatgt
     8461 ggaacaatca ttaaagttat tgtgattttt tgaacttaat gaaactaaac aataaatttg
     8521 agatactttt ttgtcatttt tatgtaacta acacaataat ctcgtacatt attaaaattt
     8581 tctatatgat agggataaag taaagagcga atgtgctgta aaagtttttc caaggtgata
     8641 ttacataaag gtataaaggg taaagattaa tgagttgtca tgtaaaggac gatgatgtat
     8701 aaatcatggt taattacgga agcattaata ttaatctgag aagctataaa gaattatttt
     8761 taaaagcgac aatattaaat acgacgcatt tatttaggag tggcaaacgt atgaatggga
     8821 aaaaggcaaa tacgataaac agatacaaat attttcatca tgtcaatcat caaaaaattc
     8881 aacaaagttc taaaaagacg ctgtgggcat cactaatcat cacattgtta tttacagtga
     8941 ttgaatttgt cggaggttta gtatctaatt cattggcatt actgtcagat tcatttcata
     9001 tgcttagtga tgtattagca cttggtttat ctatgttggc catttatttt gcaagtaaaa
     9061 agccgactgc acgatacaca tttggatatt taagatttga gatattagtt gcatttttaa
     9121 atggtttagc attaattgta atttcaatct ggattttata tgaagctatt gtacgtatta
     9181 tttatccgca accaattgaa agtggcatta tgtttatgat tgctagtatt ggtttactcg
     9241 ttaatattat tttgacaatt atccttgtaa ggtctttaaa acaagaagac aatatcaata
     9301 ttcaaagtgc attatggcat ttcatgggag acttattgaa ctctattggt gtcatcgttg
     9361 cagttgtatt gatttacttt acaggatggc gcatcatcga cccaatcatt agtattgtaa
     9421 tttcactcat cattttacgt ggtggttata aaattacgcg taatgcgtgg ttaattttaa
     9481 tggaaagtgt gcctcaacat ttggatactg atcaaattat ggcagatatt aaaaacatag
     9541 atggcatatt agatgtacat gaatttcatt tgtggagtat tacaacagag cattattcat
     9601 taagtgccca tgttgtgtta gataaaaaat atgagggtga tgattatcaa gcgattgatc
     9661 aagtatcatc attgttgaaa gaaaaatatg gcattgcaca ttcaacgttg caaattgaaa
     9721 acttgcaatt gaatccatta gatgagccat acttcgacaa attaacataa ataaaacatt
     9781 gtacaggctg ggacataaat caatgttcta tgctctacga agttatattg gcagtagttg
     9841 actgaacgaa aatgcgcttg taacaagctt ttttcaattc tagtcagggg ccccaacaca
     9901 gagaatttcg aaaagaaatt ctacaggcaa tgcaagttgg ggtgtgggcc ccaacacaga
     9961 gaatttcgaa aagaaattct acaggcaatg caagttgggg tgtgggcccc aacaaagaga
    10021 aattggattc ccaatttcta cagacaatgc aagttggggt gggacaacga aataaatttt
    10081 gcgaaaatat catttctgtc ccactcccac gtttgttttg gattatgttg catcatttaa
    10141 atgattttcg tcaatttctt tgatgctatc tacatctaac acgacatctt taggtttcaa
    10201 aatatgaata tgtttttcat catttgtatg taaaatgcgt tctatgatgt atctttgacc
    10261 ggccattgtt tctacagcaa tctttttgtt tctagctaaa cttgctacga cagattcttt
    10321 atccataatg atagccccct atatatatgt ttatttactt ataccctaac atgattttta
    10381 tactctttga aaa
//
LOCUS       DAHHSC010000080        10345 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.080, whole genome shotgun sequence.
ACCESSION   DAHHSC010000080 DAHHSC010000000
VERSION     DAHHSC010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 10345)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10345)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10345
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.080"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            94..669
                     /locus_tag="NBI57_001509"
     CDS             94..669
                     /locus_tag="NBI57_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HCD1822451.1"
                     /translation="MSFHQVEEWFEIFRQFGYLPGFILLYIRAIIPVFPLALYILINI
                     QAYGPILGILISWLGLISGTFTVYLICKRLVNTERMQRIKQRTAVQRLISFIDRQGLI
                     PLFILLCFPFTPNTLINFVASLSHIRPKYYFIVLASSKLVSTIILGYLGKEITTILTH
                     PLRGILMLVVLVVFWIVGKKLEQHFMGSKKE"
     gene            674..1198
                     /gene="lepB"
                     /locus_tag="NBI57_001510"
     CDS             674..1198
                     /gene="lepB"
                     /locus_tag="NBI57_001510"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HCD1822452.1"
                     /translation="MKKVVKYLISLILAIIIVLFVQTFVIVGHVIPNNDMSPTLNKGD
                     RVIVNKIKVTFNQLNNGDIITYRRGNEIYTSRIIAKPGQSMAFRQGQLYRDDRPVDTS
                     YAKNRKIKDFSLRNFKELDGDIIPPNNFVVLNDHDNNQHDSRQFGLIDKKDIIGNISL
                     RYYPFSKWTIQFKS"
     gene            1214..1789
                     /gene="lepB"
                     /locus_tag="NBI57_001511"
     CDS             1214..1789
                     /gene="lepB"
                     /locus_tag="NBI57_001511"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002465318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HCD1822453.1"
                     /translation="MKKELLEWIISIAVAFVILFIVGKFIVTPYTIKGESMDPTLKDG
                     ERVAVNIIGYKTGGLEKGNVVVFHANKNDDYVKRVIGVPGDKVEYKNDTLYVNGKKQD
                     EPYLNYNLKHKQGDYITGTFQVKDLPNANPKSNVIPKGKYLVLGDNREVSKDSRAFGL
                     IDEDQIVGKVSFRFWPFSEFKHNFNPENTKN"
     gene            1949..5422
                     /gene="addB"
                     /locus_tag="NBI57_001512"
     CDS             1949..5422
                     /gene="addB"
                     /locus_tag="NBI57_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddB"
                     /protein_id="HCD1822454.1"
                     /translation="MTLHAYLGRAGTGKSTKMLTEIKQKMKADPLGDPIILIAPTQST
                     FQLEQAFVNDPELNGSLRTEVLHFERLSHRIFQEVGSYSEQKLSKAATEMMIYNIVQE
                     QQKYLKLYQSQAKYYGFSEKLTEQIQDFKKYAVTPEHLEHFIADKNMQTRTKNKLEDI
                     ALIYREFEQRIQNEFITGEDSLQYFIDCMPKSEWLKRADIYIDGFHNFSTIEYLIIKG
                     LIKYAKSVTIILTTDGNHDQFSLFRKPSEVLRHIEEIANELNISIERQYFNQLYRFNN
                     QDLKHLEQEFDVLQINRVACQGHINILESATMREEINEIARRIIVDIRDKQLRYQDIA
                     ILYRDESYAYLFDSILPLYNIPYNIDTKRSMTHHPVMEMIRSLIEVIQSNWQVNPMLR
                     LLKTDVLTASYLKSAYLVDLLENFVLERGIYGKRWLDDELFNVEHFSKMGRKAHKLTE
                     DERNTFEQVVKLKKDVIDKILHFEKQMSQAETVKDFATAFYESMEYFELPNQLMTERD
                     ELDLNGNHEKAEEIDQIWNGLIQILDDLVLVFGDEPMSMERFLEVFDIGLEQLEFVMI
                     PQTLDQVSIGTMDLAKVDNKQHVYLVGMNDGTMPQPVTASSLITDEEKKYFEQQANVE
                     LSPTSDILQMDEAFVCYVAMTRAKGDVTFSYSLMGSSGDDKEISPFLNQIQSLFNQLE
                     ITNIPQYHEVNPLSLMQHAKQTKITLFEALRAWLDDEIVADSWLDAYQVIRDSDHLNQ
                     GLDYLMSALTFDNETVKLGETLSKDLYGKEINASVSRFEGYQQCPFKHYASHGLKLNE
                     RTKYELQNFDLGDIFHSVLKYISERINGDFKQLDLKKIRQLTNEALEEILPKVQFNLL
                     NSSAYYRYLSRRIGAIVETTLSALKYQGTYSKFMPKHFETSFRRKPRTNDELIAQTLT
                     TTQGIPINIRGQIDRIDTYTKNDTSFVNIIDYKSSEGSATLDLTKVYYGMQMQMMTYM
                     DIVLQNKQRLGLTDIVKPGGLLYFHVHEPRIKFKSWSDIDEDKLEQDLIKKFKLSGLV
                     NADQTVIDALDIRLEPKFTSDIVPVGLNKDGSLSKRGSQVADEATIYKFIQHNKENFI
                     ETASNIMDGHTEVAPLKYKQKLPCAFCSYQSVCHVDGMIDSKRYRTVDETINPIEAIQ
                     NININDEFGGE"
     gene            5426..9079
                     /gene="addA"
                     /locus_tag="NBI57_001513"
     CDS             5426..9079
                     /gene="addA"
                     /locus_tag="NBI57_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HCD1822455.1"
                     /translation="MTIPEKPQGVIWTDAQWQSIYATGQDVLVAAAAGSGKTAVLVER
                     IIQKILRDGIDVDRLLVVTFTNLSAREMKHRVDQRIQEASIADPANAHLKNQRIKIHQ
                     AQISTLHSFCLKLIQQHYDVLNIDPNFRTSSEAENILLLEQTIDEVIEQHYDILDPAF
                     IELTEQLSSDRSDDQFRMIIKQLYFFSVANPNPTNWLDQLVTPYEEEAQQAQLIQLLT
                     DLSKVFITAAYDALNKAYDLFSMMDGVDKHLAVIEDERRLMGRVLEGGFIDIPYLTDH
                     EFGARLPNVTAKIKEANEMMVDALEDAKLQYKKYKSLIDKVKNDYFSREADDLKADMQ
                     QLAPRVKYLARIVKDVMSEFNRKKRSKNILDFSDYEHFALQILTNEDGSPSEIAESYR
                     QHFQEILVDEYQDTNRVQEKILSCIKTGDEHNGNLFMVGDVKQSIYKFRQADPSLFIE
                     KYQRFTIDGDGTGRRIDLSQNFRSRKEVLSTTNYIFKHMMDEQVGEVKYDEAAQLYYG
                     APYDESDHPVNLKVLVEADQEHSDLTGSEQEAHFIVEQVKDILEHQKVYDMKTGSYRS
                     ATYKDIVILERSFGQARNLQQAFKNEDIPFHVNSREGYFEQTEVRLVLSFLRAIDNPL
                     QDIYLVGLMRSVIYQFKEDELAQIRILSPNDDYFYQSIVNYINDEAADAILVDKLKMF
                     LSDIQSYQQYSKDHPVYQLIDKFYNDHYVIQYFSGLIGGRGRRANLYGLFNKAIEFEN
                     SSFRGLYQFIRFIDELIERGKDFGEENVVGPNDNVVRMMTIHSSKGLEFPFVIYSGLS
                     KDFNKRDLKQPVILNQQFGLGMDYFDVDKEMAFPSLASVAYKAVAEKELVSEEMRLVY
                     VALTRAKEQLYLIGRVKNDKSLLELEQLSISGEHIAVNERLTSPNPFHLIYSILSKHQ
                     SASIPDDLKFEKDIAQVEDSSRPNVNISIIYFEDVSTETILDNNEYRSVNQLETMQNG
                     NEDVKAQIKHQLDYQYPYVNDTKKPSKQSVSELKRQYETEESGTSYERVRQYRIGFST
                     YERPKFLSEQGKRKANEIGTLMHTVMQHLPFKKERISEVELHQYIDGLIDKHIIEADA
                     KKDIRMDEIMTFINSELYSIIAEAEQVYRELPFVVNQALVDQLPQGDEDVSIIQGMID
                     LIFVKDGVHYFVDYKTDAFNRRRGMTDEEIGTQLKNKYKIQMKYYQNTLQTILNKEVK
                     GYLYFFKFGTLQL"
     gene            9245..10147
                     /locus_tag="NBI57_001514"
     CDS             9245..10147
                     /locus_tag="NBI57_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HCD1822456.1"
                     /translation="MKFLSFKYNDKTSYGVKVKREDAVWDLTQVFADFAEGDFHPKTL
                     LAGLQQNHTLDFQEQVRKAVVAAEDSGKAEDYKISFNDIEFLPPVTPPNNVIAFGRNY
                     KDHANELNHEVEKLYVFTKAASSLTGDNATIPNHKDITDQLDYEGELGIVIGKSGEKI
                     PKALALDYVYGYTIINDITDRKAQSEQDQAFLSKSLTGGCPMGPYIVTKDELPLPENV
                     NIVTKVNNEIRQDGNTGEMILKIDELIEEISKYVALHPGDIIATGTPAGVGAGMQPPK
                     FLQPGDEVKVTIDNIGTLTTYIAK"
BASE COUNT         3757 a         1434 c         1877 g         3277 t
ORIGIN      
        1 attagcatga ttacatgtta atgtttcttt acttagtaat taactttata atgtaagaat
       61 aattatcttc aaccaaagaa agggattgat gatttgtcgt ttcatcaagt agaagaatgg
      121 tttgagatat ttcgacagtt tggttattta cctggattta tattgttata tattagagcg
      181 ataattccag tatttccttt agcactctat attttaatta acattcaagc ttatggacct
      241 attttaggta tattgattag ttggcttgga ttaatttctg gaacatttac agtctatttg
      301 atctgtaaac gattggtgaa cactgagagg atgcagcgaa ttaaacaacg tactgctgtt
      361 caacgcttga ttagttttat tgatcgccaa ggattaatcc cattgtttat tttactttgt
      421 tttcctttta cgccaaatac attaataaat tttgtagcga gtctatctca tattagacct
      481 aaatattatt tcattgtttt ggcatcatca aagttagttt caacaattat tttaggttat
      541 ttaggtaagg aaattactac aattttaacg catcctttaa gagggatatt aatgttagtt
      601 gtgttggttg tattttggat tgttggaaaa aagttagaac agcattttat gggatcgaaa
      661 aaggagtgac atcgtgaaaa aagttgtaaa atatttgatt tcattgatac ttgctattat
      721 cattgtactg ttcgtacaaa cttttgtaat agttggtcat gtcattccga ataatgatat
      781 gtcaccaacc cttaacaaag gggatcgtgt tattgtaaat aaaattaaag ttacatttaa
      841 tcaattgaat aatggtgata tcattacata taggcgtggt aacgagatat atactagtcg
      901 aattattgcc aaacctggtc aatcaatggc gtttcgtcag ggacaattat accgtgatga
      961 ccgaccggtt gacacatctt atgccaagaa cagaaaaatt aaagatttta gtttgcgcaa
     1021 ttttaaagaa ttagatggag atattatacc gcctaacaat tttgttgtgc taaatgatca
     1081 tgataacaat cagcatgatt ctagacaatt tggtttaatt gataaaaagg atattattgg
     1141 taatataagt ttgagatatt atcctttttc aaaatggacg attcagttca aatcttaaaa
     1201 agaggtgtca aaattgaaaa aagaattatt ggaatggatt atttcaattg cagtcgcttt
     1261 tgtcatttta tttatagtag gtaaatttat tgttacacca tatacaatta aaggtgaatc
     1321 aatggatcca actttgaaag atggcgagcg agtagctgta aacattattg gatataaaac
     1381 aggtggtttg gaaaaaggta atgtagttgt cttccatgca aacaaaaatg atgactatgt
     1441 taaacgtgtc atcggtgttc ctggtgataa agtagaatat aaaaatgata cattatatgt
     1501 caatggtaaa aaacaagatg aaccatattt aaactataat ttaaaacata aacaaggtga
     1561 ttacattact gggactttcc aagttaaaga tttaccgaat gcgaatccta aatcaaatgt
     1621 cattccaaaa ggtaaatatt tagttcttgg agataatcgt gaagtaagta aagatagccg
     1681 tgcgtttggc ctcattgatg aagaccaaat tgttggtaaa gtttcattta gattctggcc
     1741 atttagtgaa tttaaacata atttcaatcc tgaaaatact aaaaattaat atgaaacaaa
     1801 tacaacatcg tttgtcggtt ttaatactga taaacgatgt tttattttgt tagtaccaca
     1861 ataaaagcta agttcgaaat gaacttataa taaatcaatc acaatcactt tgtgttaaaa
     1921 tatgtgtcaa aggaagtgag ggtttgtcat gacattacat gcttatttag gtagagcggg
     1981 aacaggtaag tctacgaaaa tgttgaccga aataaaacaa aaaatgaaag cagatccgct
     2041 tggagatcca atcattttaa ttgcgccaac tcaaagtaca tttcaattag aacaagcctt
     2101 tgtcaatgat ccggaattaa atggtagttt aagaacagaa gtgttgcatt ttgaacgatt
     2161 aagtcatcgt attttccaag aagttggtag ttatagcgaa caaaagttat ctaaagctgc
     2221 aacggaaatg atgatttata acattgttca agaacaacaa aagtatttaa aactttatca
     2281 atcacaagca aaatattatg ggtttagtga aaaattaaca gaacaaattc aagattttaa
     2341 aaaatatgca gtaacgcctg aacatttaga acactttatt gctgataaaa atatgcaaac
     2401 tcgaactaaa aataagttag aggatattgc tttaatatac cgtgagttcg aacaacgcat
     2461 tcaaaacgag tttattactg gtgaggattc attacaatat tttattgatt gtatgccgaa
     2521 atcagagtgg ctaaaacgtg ctgatatata tattgatggt tttcacaact tttcaacgat
     2581 tgagtattta ataatcaaag gattaattaa atatgcgaag agtgtcacaa ttatattgac
     2641 gacagatggt aaccacgatc aatttagttt atttagaaaa ccatcggaag tgttacgaca
     2701 tattgaagaa atagcaaatg aactcaatat ttctattgaa cgtcaatatt tcaaccaatt
     2761 atatcgcttc aataatcaag atttaaagca tcttgaacaa gaatttgatg tacttcaaat
     2821 caatcgagtg gcatgtcaag gtcatatcaa tattttagaa tctgcgacta tgagagagga
     2881 aataaatgaa attgcgcgac gtatcatcgt tgatattcgt gataagcaat tacgatatca
     2941 agatattgca attttatatc gtgacgagtc ttatgcttat ttatttgatt ccatattacc
     3001 gctttataat attccttata acattgatac aaagcgttcg atgacacatc atccggtcat
     3061 ggaaatgatt cgttcattga ttgaagttat tcaatctaat tggcaagtga atccaatgct
     3121 acgcttattg aagactgatg tgttaacggc atcatatcta aaaagtgcat acttagttga
     3181 tttacttgaa aattttgtac ttgaacgtgg tatatacggt aaacgttggt tagatgatga
     3241 gctatttaat gtcgaacatt ttagcaaaat ggggcgtaaa gcgcataaac tgaccgaaga
     3301 tgaacgtaac acatttgaac aagtcgttaa gttaaagaaa gatgtcattg ataaaatttt
     3361 acattttgaa aagcaaatgt cacaagcgga aactgtaaaa gactttgcaa ctgcttttta
     3421 tgaaagtatg gaatatttcg aactgccaaa tcaattgatg acagagcgag atgaacttga
     3481 tttaaatggt aatcatgaaa aggcggagga aattgatcaa atatggaatg gcttaattca
     3541 aatccttgac gacttagttc tagtatttgg agatgaacca atgtcgatgg aacgtttctt
     3601 agaagtattt gatattggtt tagaacaatt agaatttgtc atgattccac aaacattaga
     3661 tcaagttagt attggtacga tggatttggc taaagtcgac aataagcaac atgtttactt
     3721 agttggaatg aacgacggca ccatgccaca accagtaact gcatcaagtt taattactga
     3781 tgaagaaaag aaatattttg aacaacaagc aaatgtagag ttgagtccta catcagatat
     3841 tttacagatg gatgaagcat ttgtttgcta tgttgctatg actagagcta agggagatgt
     3901 tacattttct tacagtctaa tgggatcaag tggtgatgat aaggagatca gcccattttt
     3961 aaatcaaatt caatcattgt tcaaccaatt ggaaattact aacattcctc aataccatga
     4021 agttaatcca ttgtcactaa tgcaacatgc taagcaaacc aaaattacat tatttgaagc
     4081 attgcgtgct tggttagatg atgaaattgt ggctgatagt tggttagatg cttatcaagt
     4141 aattagagat agcgatcatt taaatcaagg tttagattat ttaatgtcag cattaacgtt
     4201 tgacaatgaa actgtaaaat taggtgaaac gttgtctaaa gatttatatg gtaaggaaat
     4261 caatgccagt gtatctcgtt ttgaaggtta tcaacaatgc ccatttaaac actatgcttc
     4321 acatggtctg aaactaaatg aacgaacgaa atatgaactt caaaactttg atttaggtga
     4381 tattttccat tccgttttaa aatatatatc tgaacgtatt aatggcgatt ttaaacaatt
     4441 agacctgaaa aaaataagac aattaacgaa tgaagcattg gaagaaattt tacctaaagt
     4501 tcagtttaat ttattaaatt cttcagctta ctatcgttat ttatcaagac gcattggcgc
     4561 tattgtagaa acaacactaa gcgcattaaa atatcaaggc acgtattcaa agtttatgcc
     4621 aaaacatttt gagacaagtt ttagaaggaa accaagaaca aatgacgaat taattgcaca
     4681 aacattaacg acaactcaag gtattccaat taatattaga gggcaaattg accgtatcga
     4741 tacgtataca aagaatgata caagttttgt taatatcatt gactataaat cctctgaagg
     4801 tagtgcgaca cttgatttaa cgaaagtata ttatggtatg caaatgcaaa tgatgacata
     4861 catggatatc gttttacaaa ataaacaacg ccttggatta acagatattg tgaaaccagg
     4921 tggattatta tacttccatg tacatgaacc tagaattaaa tttaaatcat ggtctgatat
     4981 tgatgaagat aaactagaac aagatttaat taaaaagttt aagctgagtg gtttagtgaa
     5041 tgcagaccaa actgttattg atgcattgga tattcgttta gaacctaaat tcacttcaga
     5101 tattgtacca gttggtttga ataaagatgg ctctttgagt aaacgaggca gccaagtggc
     5161 agatgaagca acaatttata aattcattca gcataacaaa gagaatttta tagaaacagc
     5221 ttcaaatatt atggatggac atactgaagt tgcaccatta aagtacaaac aaaaattgcc
     5281 atgtgctttt tgtagttatc aatcggtatg tcatgtagat ggcatgattg atagtaagcg
     5341 atatcgaact gtagatgaaa caataaatcc aattgaagca attcaaaata ttaacattaa
     5401 tgatgaattt gggggtgagt aatagatgac aattccagag aaaccacaag gcgtgatttg
     5461 gactgacgcg caatggcaaa gtatttacgc aactggacaa gatgtacttg ttgcagccgc
     5521 ggcaggttca ggtaaaacag ctgtactagt tgagcgtatt atccaaaaga ttttacgtga
     5581 tggcattgat gtcgatcgac ttttagtcgt aacgtttaca aacttaagcg cacgtgaaat
     5641 gaagcatcgt gtagaccaac gtattcaaga ggcatcgatt gctgatcctg caaatgcaca
     5701 cttgaaaaac caacgcatca aaattcatca agcacaaata tctacacttc atagtttttg
     5761 cttgaaatta attcaacagc attatgatgt attaaatatt gacccgaact ttagaacaag
     5821 cagtgaagct gaaaatattt tattattaga acaaacgata gatgaggtca tagaacaaca
     5881 ttacgatatc cttgatcctg cttttattga attaacagaa caattgtctt cagatagaag
     5941 tgatgatcag tttcgaatga ttattaaaca attgtatttc tttagcgttg caaatccaaa
     6001 tcctacaaat tggttggatc aattggtgac accatacgaa gaagaagcac aacaagcgca
     6061 acttattcaa ctactaacag acttatctaa agtatttatc acagctgcct atgatgcttt
     6121 aaataaggcg tatgatttgt ttagtatgat ggatggcgtc gataaacatt tagctgttat
     6181 agaagatgaa cgacgtttaa tggggcgtgt tttagaaggt ggttttattg atatacctta
     6241 tttaactgat cacgaatttg gcgcgcgttt gcctaatgta acagcgaaaa ttaaagaagc
     6301 aaatgaaatg atggtcgatg ccttagaaga tgctaaactt cagtataaaa aatataaatc
     6361 attaattgat aaagtgaaaa atgattactt ttcaagagaa gctgatgatt tgaaagctga
     6421 tatgcaacaa ttggcgccac gagtaaagta ccttgcgcgt attgtgaaag atgttatgtc
     6481 agaattcaat cgaaaaaagc gtagcaaaaa tattctggat ttttctgatt atgaacattt
     6541 tgcattacaa attttaacta atgaggatgg ttcgccttca gaaattgccg aatcataccg
     6601 tcaacacttt caagaaatat tggtcgatga gtatcaagat acgaaccggg ttcaagagaa
     6661 aatactatct tgcatcaaaa cgggtgatga acataatggt aatttattta tggttggaga
     6721 tgttaagcaa tccatttata aatttagaca agctgatcca agtttattta ttgaaaagta
     6781 tcaacgcttt actatagatg gagatggcac tggacgtcga attgatttgt cgcaaaactt
     6841 ccgttctcga aaagaagtac tgtcaacgac taactatata ttcaaacata tgatggatga
     6901 acaagtcggt gaagtaaaat atgatgaagc ggcacagttg tattatggtg caccatatga
     6961 tgaatcggac catccagtaa acttaaaagt gcttgttgaa gcggatcaag aacatagtga
     7021 tttaactggt agtgaacaag aagcgcattt tatagtagaa caagttaaag atatcttaga
     7081 acatcaaaaa gtttatgata tgaaaacagg aagctataga agtgcgacat acaaagatat
     7141 cgttattcta gaacgcagct ttggacaagc tcgcaattta caacaagcct ttaaaaatga
     7201 agatattcca ttccatgtga atagtcgtga aggttacttt gaacaaacag aagtccgctt
     7261 agtattatca tttttaagag cgatagataa tccattacaa gatatttatt tagttgggtt
     7321 aatgcgctcc gttatatatc agttcaaaga agacgaatta gctcaaatta gaatattgag
     7381 tccaaatgat gactacttct atcaatcgat tgtaaattac attaatgacg aagcagcaga
     7441 tgctatttta gttgataaat taaaaatgtt tttatcagat attcaaagtt accaacaata
     7501 tagtaaagat catccggtgt atcagttaat tgataaattt tataatgatc attatgttat
     7561 tcaatacttt agtggactta ttggtggacg tggacgacgt gcaaaccttt atggtttatt
     7621 taataaagct atcgagtttg agaattcaag ttttagaggt ttatatcaat ttattcgttt
     7681 tatcgatgaa ttgattgaaa gaggcaaaga ttttggtgag gaaaatgtag ttggtccaaa
     7741 cgataatgtt gttagaatga tgacaattca tagtagtaaa ggtctagagt ttccatttgt
     7801 catttattct ggattgtcaa aagattttaa taaacgtgat ttgaaacaac cagttatttt
     7861 aaatcagcaa tttggtctcg gaatggatta ttttgatgtg gataaagaaa tggcatttcc
     7921 atctttagct tcggttgcat ataaagctgt tgccgaaaaa gaacttgtgt cagaagaaat
     7981 gcgattagtc tatgtagcat taacaagagc gaaagaacaa ctttatttaa ttggtagagt
     8041 gaaaaatgat aaatcgttac tagaactaga gcaattgtct atttctggtg agcacattgc
     8101 tgtcaatgaa cgattaactt caccaaatcc gttccatctt atttatagta ttttatctaa
     8161 acatcaatct gcgtcaattc cagatgattt aaaatttgaa aaagatatag cacaagttga
     8221 agatagtagt cgtccgaatg taaatatttc aattatatac tttgaagatg tgtctacaga
     8281 aaccatttta gataataatg aatatcgttc ggttaatcaa ttagaaacta tgcaaaatgg
     8341 taatgaggat gttaaagcac aaattaaaca ccaacttgat tatcaatatc catatgtaaa
     8401 tgatactaaa aagccatcaa aacaatctgt ttctgaattg aaaaggcaat atgaaacaga
     8461 agaaagtggc acaagttacg aacgagtaag acaatatcgt atcggttttt caacgtatga
     8521 acgacctaaa tttctaagtg aacaaggtaa acgaaaagcg aatgaaattg gtacgttaat
     8581 gcatacagtg atgcaacatt taccattcaa aaaagaacgc atatctgaag ttgagttaca
     8641 tcagtatatc gatggattaa tcgataaaca tattatcgaa gcagatgcga aaaaagatat
     8701 ccgtatggat gaaataatga catttatcaa tagtgagtta tattcgatta ttgctgaagc
     8761 agagcaagtt tatcgtgaat taccgtttgt agttaaccaa gcattagttg accaattgcc
     8821 acaaggagac gaagacgtct caattattca aggtatgatt gacttaatct ttgttaaaga
     8881 tggtgtgcat tattttgtag actataaaac cgatgcattt aatcgtcgcc gtgggatgac
     8941 agatgaagaa attggtacac aattaaaaaa taaatataag atacagatga aatattatca
     9001 aaatacgctt caaacgatac ttaataaaga agttaaaggt tatttatact tcttcaaatt
     9061 tggtacattg caactgtagt attttgattt tcaaaagaat aaaaaataat ttcgattaag
     9121 tgcaaagtcc ttgtagcaga atgaacacaa ctcattttca aaattgtctt acttatttat
     9181 ttgttatttg ataacgaaaa aagttataat gtgaattaag ataaagatga ggagttgaga
     9241 atgaatgaaa ttcttatcat tcaagtataa tgacaaaact tcatatggcg ttaaagtaaa
     9301 acgcgaagat gctgtatggg atttaacaca agtatttgct gactttgcag aaggagattt
     9361 ccatcctaaa acattgttag ctggtttaca acaaaatcat actttagatt ttcaagaaca
     9421 agtacgtaaa gcagttgtag cagcagaaga tagcggcaaa gctgaagact ataaaatttc
     9481 atttaatgac attgaattct taccaccagt aacacctccg aataatgtga ttgcttttgg
     9541 tagaaattac aaagatcatg cgaacgaatt aaatcatgaa gtagaaaaat tatatgtatt
     9601 tacaaaagca gcgtcatctt taacaggaga taatgcaaca attccaaatc ataaagatat
     9661 tactgatcaa ttagattatg aaggtgaatt aggtattgtt attggtaagt ctggtgaaaa
     9721 gattccaaaa gcattagctt tagattatgt ttacggctat acaattatta acgatatcac
     9781 tgatcgcaaa gcacaaagtg aacaagatca agcattttta tcaaaaagtt taactggcgg
     9841 ttgcccaatg ggtccttata tcgttactaa agacgaacta ccattacctg aaaatgtaaa
     9901 tattgttaca aaagttaaca atgaaattag acaagatggt aacactggcg aaatgattct
     9961 taaaattgat gaattaatag aagaaatttc aaaatatgtt gcactacatc cgggagatat
    10021 tattgcaact ggtacaccag caggcgttgg tgcaggtatg caaccaccta aatttttaca
    10081 accaggtgat gaagttaaag tgactattga taatattgga acgctgacaa cttatatcgc
    10141 taaataatta tcatttaaaa agctaaccag tctttatata gattggttag ttttttcttg
    10201 cttttctaaa aaggtgttaa agataaatta tttataatgt taccattttg agatgaaagt
    10261 gaaatattga tattaagaag tagttgatta ttttacagca gattcacaat attctaataa
    10321 ggaataaaat gtcatgttct tcctc
//
LOCUS       DAHHSC010000081        10339 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.082, whole genome shotgun sequence.
ACCESSION   DAHHSC010000081 DAHHSC010000000
VERSION     DAHHSC010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 10339)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10339)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10339
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.082"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            345..1799
                     /locus_tag="NBI57_001515"
     CDS             345..1799
                     /locus_tag="NBI57_001515"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HCD1822457.1"
                     /translation="MSDRIRVRYAPSPTGYLHIGNARTALFNYLYAKHYNGDFVIRIE
                     DTDKKRNLEDGETSQFDNLKWLGLDWDESVDKDNGYGPYRQSERQHIYQPLIDQLLAE
                     DKAYKCYMTEEELEAEREAQIARGEMPRYGGQHAHLTEEQRQQFEAEGRQPSIRFRVP
                     QNQTYSFDDMVKGNISFDSNGIGDWVIVKKDGIPTYNFAVAIDDHYMQISDVIRGDDH
                     ISNTPKQIMIYEAFGWEPPRFGHMSLIVNEERKKLSKRDGQILQFIEQYRDLGYLPEA
                     LFNFIALLGWSPEGEEEIFSKEEFIKIFDEKRLSKSPAFFDKQKLAWVNNQYMKQKDT
                     ETVFQLALPHLIKANLIPEVPSEEDLSWGRKLIALYQKEMSYAGEIVPLSEMFFKEMP
                     ALGEEEQQVINGEQVPELMTHLFSKLEALEPFEAAEIKKTIKEVQKETGIKGKQLFMP
                     IRVAVTGQMHGPELPNTIEVLGKEKVLNRLKQYK"
     gene            2222..2869
                     /gene="cysE"
                     /locus_tag="NBI57_001516"
     CDS             2222..2869
                     /gene="cysE"
                     /locus_tag="NBI57_001516"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HCD1822458.1"
                     /translation="MILLKRMRDDIKMVFEQDPAARSTLEVITTYAGLHAVWSHLIAH
                     KLYNQKKYVAARAISQISRFFTGIEIHPGAKIGKRLFIDHGMGVVIGETCTIGDNVTI
                     YQGVTLGGTGKERGKRHPDIGDNVLIAAGAKVLGNIKINSNVNIGANSVVLQSVPSYS
                     TVVGIPGHIVKQDGVRVGKTFDHRHLPDPIYEQIKHLERQLEKTRNGEIQDDYII"
     gene            2853..4253
                     /locus_tag="NBI57_001517"
     CDS             2853..4253
                     /locus_tag="NBI57_001517"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000631968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HCD1822459.1"
                     /translation="MITLYNTLTRQKEVFKPIEPGKVKMYVCGPTVYNYIHIGNARPA
                     INYDVVRRYFEYQGYNVEYVSNFTDVDDKLIKRSQELNQSVPEIAEKYIAAFHEDVGA
                     LNVRKATSNPRVMDHMDDIIQFIKDLVDQGYAYESGGDVYFRTRKFEGYGKLSHQSID
                     DLKVGARIDAGEHKEDALDFTLWKKAKPGEISWNSPFGEGRPGWHIECSVMAFHELGP
                     TIDIHAGGSDLQFPHHENEIAQSEAHNHAPFANYWMHNGFINIDNEKMSKSLGNFILV
                     HDIIKEVDPDVLRFFMISVHYRSPINYNLELVESARSGLERIRNSYQLIEERAQIATN
                     IENQQTYIDQIDAILNRFETVMNDDFNTANAITAWYDLAKLANKYVLENTTSTEVIDK
                     FKAVYQIFSDVLGVPLKSKNADELLDEDVEKLIEERNEARKNKDFARADEIRDMLKSQ
                     NIILEDTPQGVRFKRG"
     gene            4246..4650
                     /locus_tag="NBI57_001518"
     CDS             4246..4650
                     /locus_tag="NBI57_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HCD1822460.1"
                     /translation="MDNQQDNHIKLLNPLTLAYMGDAVLDQYVRTYIVLKLKSKPNKL
                     HQMSKKYVSAKSQAQTLEYLMEQEWFTDEEMDILKRGRNAKSHTKAKNTDVQTYRKSS
                     AIEAVIGFLYLEKREERLEALLNKIITIVNER"
     gene            4658..5404
                     /gene="rlmB"
                     /locus_tag="NBI57_001519"
     CDS             4658..5404
                     /gene="rlmB"
                     /locus_tag="NBI57_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HCD1822461.1"
                     /translation="MEDTVIVGRHAVREAIITGHPINKILIQEGIKKQQINEILKNAK
                     DQKIIVQTVPKSKLDFLANAPHQGVAALIAPYEYADFDQFLKQQKEKEGLSTVLILDG
                     LEDPHNLGSILRTADATGVDGVIIPKRRSVTLTQTVAKASTGAIEHVPVIRVTNLAKT
                     IDELKDNGFWVAGTEANNATDYRNLEADMSLAIVIGSEGQGMSRLVSDKCDFYIKIPM
                     VGHVNSLNASVAASLMMYEVFRKRHDVGEI"
     gene            5404..5928
                     /locus_tag="NBI57_001520"
     CDS             5404..5928
                     /locus_tag="NBI57_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HCD1822462.1"
                     /translation="MKERYLIIDGYNMIGQSPTLSAIAKENLEEARMQLIDAIANYNA
                     VISDEIICVFDAYDQSGVEREYMYHGVKTIFTKEKETADSFIERYVYELYDKHTKHIA
                     VVTSDMSEQHAIFGSGAYRISSREMWRDLKENEIDVSKSLDDISENKPRTRIPLSSEI
                     LAEFEKIRRGHHKK"
     gene            6009..6578
                     /locus_tag="NBI57_001521"
     CDS             6009..6578
                     /locus_tag="NBI57_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HCD1822463.1"
                     /translation="MKYDLTTQDSTIKRNNSINDKDFEKLVMDLQPLIIRRIKTFGFN
                     HYDLEDLYQEILIRMYRSVQTFDFSGEQPFTNYVQCLITSVKYDYLRKYLATNKRMDN
                     LINEYRVTYPCAIKRFDVENNYLNKLAIKELICQFKYLSAFEKDVMYLMCEQYKPREI
                     AQLMHVKEKVIYNAIQRCKNKIKRYFKMI"
     gene            6693..6836
                     /gene="rpmG"
                     /locus_tag="NBI57_001522"
     CDS             6693..6836
                     /gene="rpmG"
                     /locus_tag="NBI57_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002451211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HCD1822464.1"
                     /translation="MRKIPLNCEACGNRNYNVPKQEGSATRLTLKKYCPKCNAHTIHK
                     ESK"
     gene            6892..7074
                     /gene="secE"
                     /locus_tag="NBI57_001523"
     CDS             6892..7074
                     /gene="secE"
                     /locus_tag="NBI57_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499089.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HCD1822465.1"
                     /translation="MAKKESFFKGVKSEMEKTSWPTKEELFKYTVIVVSTVIFFLVFF
                     YALDLGITALKNLLFG"
     gene            7087..7635
                     /gene="nusG"
                     /locus_tag="NBI57_001524"
     CDS             7087..7635
                     /gene="nusG"
                     /locus_tag="NBI57_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001832303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HCD1822466.1"
                     /translation="MSEEVGAKRWYAVHTYSGYENKVKKNLEKRVESMNMTEQIFRVV
                     IPEEEETQVKDGKAKTTVKKTFPGYVLVELIMTDESWYVVRNTPGVTGFVGSAGAGSK
                     PNPLLPEEVRFILKQMGLKEKTIDVELEVGEQVRIKSGPFANQVGEVQEIETDKFKLT
                     VLVDMFGRETPVEVEFDQIEKL"
     gene            7816..8238
                     /gene="rplK"
                     /locus_tag="NBI57_001525"
     CDS             7816..8238
                     /gene="rplK"
                     /locus_tag="NBI57_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012664221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HCD1822467.1"
                     /translation="MAKKVDKVVKLQIPAGKANPAPPVGPALGQAGVNIMGFCKEFNA
                     RTQDQAGLIIPVEISVYEDRSFTFITKTPPAPVLLKKAAGIEKGSGEPNKTKVATVTK
                     DQVREIANSKMQDLNAADEEAAMRIIEGTARSMGIVVE"
     gene            8446..9138
                     /gene="rplA"
                     /locus_tag="NBI57_001526"
     CDS             8446..9138
                     /gene="rplA"
                     /locus_tag="NBI57_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HCD1822468.1"
                     /translation="MAKKGKKYQEAASKVDRTQHYSVEEAIKLAKETSIANFDASVEV
                     AFRLGIDTRKNDQQIRGAVVLPNGTGKSQSVLVFAKGDKIAEAEAAGADYVGEAEYVQ
                     KIQQGWFDFDVVVATPDMMGEVGKLGRVLGPKGLMPNPKTGTVTMDVKKAVEEIKAGK
                     VEYRAEKAGIVHASIGKVSFTDEQLIENFNTLQDVLAKAKPSSAKGTYFKSVAVTTTM
                     GPGVKIDTASFK"
     misc_feature    9226..9372
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            9410..9910
                     /locus_tag="NBI57_001527"
     CDS             9410..9910
                     /locus_tag="NBI57_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HCD1822469.1"
                     /translation="MSAIIEAKKQLVDEIAEVLSNSVSTVIVDYRGLTVAEVTDLRSQ
                     LREAGVEYKVYKNTMVRRAAEKAGIEGLDEFLTGPTAIATSSEDAVAAAKVISGFAKD
                     HEALEIKSGVMEGNVITAEEVKTVGSLPSHDGLVSMLLSVLQAPVRNFAYAVKAIGEQ
                     KEENAE"
     gene            9953..10321
                     /gene="rplL"
                     /locus_tag="NBI57_001528"
     CDS             9953..10321
                     /gene="rplL"
                     /locus_tag="NBI57_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001273587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HCD1822470.1"
                     /translation="MANHEQIIEAIKEMSVLELNDLVKAIEEEFGVTAAAPVAVAGAA
                     GGADAAAEKTEFDVELTSAGSSKIKVVKAVKEATGLGLKDAKELVDGAPKVIKEALPK
                     EEAEKLKEQLEEVGATVELK"
BASE COUNT         3860 a         1406 c         2033 g         3040 t
ORIGIN      
        1 atcaatttta acgtatttga atagttaatt tgaagtttaa ttttacagta cataatattc
       61 atttttaagt cgttgaaatg cttatgtagt gcagtcttga ttcttaggga tagctttttt
      121 aaagtgttga aaaaacaact gctttctatt taaaatttaa gcatattacc cagtgaatta
      181 tgttatgatt aaacaatgaa attattttaa ggtcatgtta aaaagcattg tttcgtttta
      241 acaataagac gagttagaca tttgttatga cggtcgaaga tagcaaatga ataatttata
      301 tgaactaact tgaaaatctg atatttaatt taggagtgaa aaaaatgagc gatcgtataa
      361 gagtaagata tgcaccaagt ccaactggtt atcttcatat tggtaatgca agaacagcat
      421 tattcaatta cttgtatgct aaacattaca acggagattt tgtgattcga attgaagata
      481 ctgataaaaa acgtaattta gaagatggag aaacatcaca atttgataat cttaaatggt
      541 taggattaga ttgggatgag tctgtagata aagacaatgg ctacggacca tatcgtcaat
      601 ctgaacgtca acatatctac caaccattaa tagatcagtt actagcagaa gataaagcat
      661 ataaatgcta tatgacagaa gaagaattag aagctgaacg tgaagcgcaa atcgctcgtg
      721 gtgaaatgcc tcgctatggt ggtcaacatg cgcatttgac tgaagaacaa cgtcaacaat
      781 ttgaagcaga aggacgccaa ccatcaattc gtttccgagt acctcaaaac caaacgtatt
      841 catttgatga tatggtaaaa ggaaatattt catttgattc aaatggtatt ggtgactggg
      901 ttatcgtaaa aaaagatggc attccaacgt acaattttgc agtagctata gatgatcatt
      961 acatgcaaat ttcagatgta attcgtggtg atgatcatat ttcaaacacg cctaaacaaa
     1021 ttatgattta tgaagcattt ggctgggagc cacctcgttt tggtcatatg tcattaattg
     1081 ttaatgaaga acgtaaaaag ttaagtaaac gtgatgggca aattttacaa tttattgagc
     1141 aatatcgtga cttaggttat ttacctgaag cgttatttaa ttttattgcg ttattaggtt
     1201 ggtctcctga aggtgaagaa gaaatctttt ctaaagaaga atttatcaaa atctttgatg
     1261 aaaagcgttt gtcaaaatca ccagcatttt tcgataagca aaaattagca tgggttaata
     1321 accaatatat gaaacaaaaa gatactgaaa cagtattcca attagcatta cctcatttaa
     1381 ttaaagcaaa tttgattcct gaggtgccgt cagaagagga tttatcttgg ggacgcaaat
     1441 taattgcgct ttatcaaaaa gaaatgagtt atgccggtga aattgtacct ttatcagaaa
     1501 tgttctttaa agaaatgcca gctcttggtg aagaagaaca acaagtgatt aatggagagc
     1561 aagtaccaga gttaatgacg cacttattca gtaaattaga agcacttgaa ccatttgaag
     1621 cggctgaaat taaaaagaca attaaagaag ttcaaaaaga aacaggaata aaaggcaagc
     1681 aattatttat gcctattcgt gttgctgtaa caggccaaat gcatggtcct gaattaccaa
     1741 atacaattga agtacttggt aaagaaaaag tgctaaaccg tttaaaacaa tataagtaat
     1801 gaaatagaca agatcaaggt agtatatact tgaattttat aacgtaacca catatgataa
     1861 agttgtatta acaaattaca acaatatgaa taaagtattt tatataaaac gaaggattag
     1921 taattaaatt tatacgatgc agagagtgta cggttgctgt gagtacaacg tagaaattaa
     1981 tgaatgcacc ttcgtaaaat gaattaaata tataatgaga gtgatgagca ttaagttgac
     2041 ttagtttcct tgataatttg gaagcgcccg caatattatt aatgttattc gctaaattca
     2101 gagtggaacc gtgcggaagc gcctctaaca atacaatttg tatgttagtg gtgctttttt
     2161 gatatttaat ttcgcaggta cttcaattat gtatttacgt agttcaatca ttgaggagga
     2221 aatgatcttg ttaaaaagaa tgagagacga tataaaaatg gtatttgagc aggatccagc
     2281 ggcacgttca acattagaag tcattacaac gtatgcaggt ttacatgcag tttggagtca
     2341 tttgattgca cataagttat acaaccaaaa aaaatatgtt gcagcacgcg cgatatctca
     2401 aatttcaaga tttttcacag gtatagaaat ccatccaggt gctaaaattg gaaagcgtct
     2461 atttatagat catggtatgg gcgttgtaat aggagaaaca tgtacaattg gtgataatgt
     2521 gacaatctat caaggcgtga cacttggtgg gacagggaaa gaaagaggga aaagacaccc
     2581 agatatagga gacaatgttt taatagcagc cggtgcgaaa gttttaggaa atattaaaat
     2641 aaattcaaat gtaaatattg gtgcaaattc agttgtttta caatcagttc caagttattc
     2701 aacggttgtt ggtataccag gacatattgt taagcaagat ggtgttcgag ttggaaaaac
     2761 atttgatcat cgccatctac ctgatccaat ttatgaacaa attaagcatt tagaacgaca
     2821 acttgaaaag actaggaatg gagagattca agatgattac attatataat acgcttacac
     2881 gtcaaaaaga agtgttcaag cctatagaac cagggaaagt aaaaatgtat gtatgtggtc
     2941 ctactgtata taactacatt catattggta acgcaagacc agcaattaat tatgacgtag
     3001 tgagacgtta ctttgaatac caaggatata atgtagaata tgtatcaaat tttacagacg
     3061 tagatgataa attaattaaa cgttctcaag aattaaatca gtctgttccc gaaattgcag
     3121 aaaaatatat cgctgctttt catgaagatg ttggtgcgtt aaatgttaga aaagcgactt
     3181 caaatccaag ggtaatggac catatggatg acattattca atttattaaa gatttggtgg
     3241 atcaaggtta tgcatatgaa agtggtggcg atgtttactt tagaacacgt aaatttgaag
     3301 gttatggtaa attaagtcat caatccatag atgacttaaa agtgggtgct cgtatagatg
     3361 caggagagca taaagaagat gcacttgatt ttacattgtg gaaaaaagcg aagcctggcg
     3421 agattagttg gaatagccca tttggtgaag gtagaccagg atggcatata gaatgttctg
     3481 taatggcatt tcatgagcta ggacctacaa ttgatataca tgcgggtggt tcagatttac
     3541 aatttccaca tcatgaaaat gaaatagcac aatcagaagc acataatcat gcgccatttg
     3601 ctaattattg gatgcataat ggtttcatta atattgataa tgaaaaaatg agtaaatcac
     3661 taggtaactt tattttagtt cacgatatta ttaaagaagt tgatccagat gtactaagat
     3721 tctttatgat tagcgtacat tatagaagcc caattaacta taatctagaa ttggtagaat
     3781 cagcacgtag tggactagag cgtattcgca atagttatca attaattgaa gagcgcgcac
     3841 aaattgctac taatattgaa aatcaacaga catatattga tcaaattgat gcgattttaa
     3901 atcgttttga aacagttatg aatgatgatt ttaatacagc taatgcaatt acagcttggt
     3961 atgatttagc aaaacttgcg aataaatatg tactagagaa cacaacatca acagaagtaa
     4021 ttgataaatt taaagcagtt tatcaaattt tcagcgatgt tttaggtgta ccgttaaaat
     4081 ctaaaaatgc agatgaatta ttggatgaag atgttgaaaa attaatcgaa gagcgtaatg
     4141 aagcaaggaa aaacaaagat tttgcacgag cagatgagat tcgagacatg ctgaaatcac
     4201 aaaacattat attagaagac acacctcaag gggttagatt taaacgtgga taatcaacaa
     4261 gataatcaca ttaaattatt gaatccattg accttagcat atatgggaga cgcagtctta
     4321 gatcaatatg tacgtaccta tatcgtttta aagcttaaaa gtaagcctaa taaactacat
     4381 caaatgtcta aaaaatatgt atctgccaaa agtcaggcgc aaacgttaga atatttaatg
     4441 gagcaagaat ggtttacaga cgaagaaatg gatattttga agcgagggcg taacgcgaaa
     4501 agtcatacta aagctaaaaa cactgatgtt caaacatatc gtaaaagttc agcgatagaa
     4561 gcagtgatag gttttcttta tttagaaaaa agagaagaac gattagaggc attattaaat
     4621 aaaataataa caatagtaaa cgaaaggtag tgacgatgtg gaagatacgg ttattgttgg
     4681 taggcatgct gttagagaag cgattattac tgggcatccg ataaataaga tattgattca
     4741 agaaggtatt aaaaagcaac aaattaatga aattttaaaa aatgcaaaag atcaaaaaat
     4801 tattgttcaa actgtaccaa aatctaaatt agatttttta gcaaatgcac cacatcaggg
     4861 tgttgcagcg cttattgcac catatgaata tgctgacttc gatcaatttt taaaacagca
     4921 aaaagaaaaa gaaggtttat cgacagtact tatattagac ggcttagaag acccgcataa
     4981 cttgggatca attttaagaa cagccgatgc aacgggagtt gatggtgtta ttattcctaa
     5041 acgtcgttca gttacactaa cgcaaacagt tgcaaaagcc tcaacaggtg caattgaaca
     5101 tgtaccagtt attcgagtga caaatttagc taaaactatc gatgaactaa aagataatgg
     5161 cttttgggta gctggcactg aagctaataa tgcaacagat tatagaaatc tagaagcgga
     5221 catgtcattg gctattgtaa ttggtagcga aggacagggt atgagtcgcc tagtaagtga
     5281 taaatgcgat ttttatatta agattccaat ggttggacat gtaaacagtt tgaatgcttc
     5341 ggttgcagca agtttaatga tgtacgaagt atttcgaaaa agacatgatg ttggagaaat
     5401 ataatgaaag aacgttactt aatcattgat ggatacaata tgataggaca atcaccaacg
     5461 ctaagcgcca ttgcaaaaga gaatttagaa gaagctagaa tgcaattaat agatgcaatt
     5521 gcaaattata atgcagttat ttcagatgaa attatttgtg ttttcgatgc ttatgaccaa
     5581 tcgggtgttg aaagagaata catgtatcat ggcgttaaaa cgatttttac caaggaaaaa
     5641 gaaacagctg atagtttcat agaacgttat gtttatgaac tttatgacaa gcatactaag
     5701 catattgcag ttgtaacaag tgatatgagt gagcaacatg ctatctttgg atcaggtgca
     5761 tatagaatat catctcgcga aatgtggaga gatttaaaag aaaatgaaat tgatgtgagt
     5821 aaatcattag atgatataag tgaaaacaag ccaagaactc gaattccgtt atcttctgaa
     5881 atccttgcag aatttgaaaa aatacgaaga ggacatcata agaaatgaca tttccgtcat
     5941 cttgaaattt tgaatgtaac aatattaaac taaccttaaa ttttagatag aaggggttag
     6001 tttaatactt gaaatatgat ttgacaactc aagacagtac aatcaaacgt aacaattcaa
     6061 taaatgataa agacttcgaa aagttagtaa tggatctaca accattaatt attcgacgca
     6121 tcaaaacatt tggatttaat cattatgatt tagaagactt atatcaagaa atacttatac
     6181 ggatgtatag gtcggtccaa acatttgatt ttagtggaga gcagcctttc acaaattatg
     6241 ttcaatgttt aattacgtct gtaaagtatg attatttgag aaaatattta gctacaaata
     6301 aaagaatgga taatttgatt aatgaatata gagttacgta tccatgtgca ataaagcgtt
     6361 ttgatgttga aaacaattat ttgaataaat tagcaattaa agagttgatt tgtcagttta
     6421 agtatttgag tgcatttgaa aaagatgtca tgtatttaat gtgtgaacaa tataagccga
     6481 gagaaattgc tcaattgatg catgtaaaag agaaagtgat ttataatgcc atacaacgat
     6541 gtaaaaataa aataaaacgt tatttcaaaa tgatttgaaa agcgccttag gacgtgaatt
     6601 gaattataac gtgttactta ctgatggttt gacatttgtt ataaatttta tgtatagtat
     6661 actggtatta taatgaataa aggtgaatta ttgtgagaaa aataccttta aattgtgaag
     6721 cttgtggcaa tagaaattat aatgttccta agcaagaagg ctcggcaaca agattaacct
     6781 taaagaaata ttgtccaaaa tgtaacgcgc acacaattca taaagaatcg aaataaatac
     6841 attcgaaata atactttgat aatatgttca aaggatttgg aggttgagca gatggctaaa
     6901 aaagaaagtt tctttaaagg cgttaagtct gaaatggaaa aaacaagttg gccgacgaaa
     6961 gaagagctat ttaaatatac tgtaattgta gtttctactg ttatattctt cttagtcttt
     7021 ttctatgcct tagatttagg aattacagca ttgaaaaatt tattatttgg ttagaggagt
     7081 gaagacatgt ctgaagaagt tggcgcaaag cgttggtatg cagtgcatac atattctgga
     7141 tatgaaaata aagttaaaaa gaatttagaa aaaagagtag aatctatgaa tatgactgaa
     7201 caaatcttta gagtagtcat accggaagaa gaagaaactc aagtaaaaga tggcaaagct
     7261 aaaacgactg ttaaaaaaac attccctgga tatgttttag tggaattaat catgacagat
     7321 gaatcatggt atgtggtaag aaatacacca ggcgttactg gttttgtagg ttctgcaggt
     7381 gcagggtcta agccaaatcc attgttacca gaagaagttc gcttcatctt aaaacaaatg
     7441 ggtcttaaag aaaagactat cgatgttgaa ctcgaagttg gcgagcaagt tcgtattaaa
     7501 tcaggtccat ttgcgaatca agttggtgaa gttcaagaaa ttgaaacaga taagtttaag
     7561 ctaacagtat tagtagatat gtttggccga gaaacaccag tagaagttga attcgatcaa
     7621 atagaaaagc tttaattaac aattaaagtt attaaactaa ccaaaagata aaaaagagta
     7681 ttgatttttt aattagaaaa gtgttaaaat tatgtggtcg cgcttttaga gcgcccattt
     7741 cgtcacgaaa tgttaagagt gggagggcaa aactgagccc tgtgaccaca tcacgatatc
     7801 aaggaggtgc acatcgtggc taaaaaagta gataaagttg ttaaattaca aattcctgca
     7861 ggtaaagcga atccagcacc accagttggt ccagcattag gtcaagcagg tgtgaacatc
     7921 atgggattct gtaaagagtt caatgcacgt actcaagatc aagcaggttt aattattccg
     7981 gtagaaatca gtgtttatga agatcgttca tttacattta ttacaaaaac tccaccggct
     8041 ccagtattac ttaaaaaagc agctggtatt gaaaaaggtt caggcgaacc aaacaaaact
     8101 aaagttgcta cagtaactaa agatcaagta cgcgaaattg ctaacagcaa aatgcaagac
     8161 ttaaacgctg ctgacgaaga agcagctatg cgtattatcg aaggtactgc acgtagtatg
     8221 ggtatcgttg tagaataatt ttacgaatat taaatttgat tacatgattt aaacgatgaa
     8281 gcagataaca gagataataa tgatgaatta taaatataat ctgaatgact agattaatga
     8341 ttgatttatt cataagatta attcttctgt tgtctgctct taacttgcat atagcaagta
     8401 atgtgggagg aaattccgct aaaaccacta aaggaggaac tataaatggc taaaaaaggt
     8461 aaaaagtatc aagaagcagc tagtaaagtt gaccgtactc agcactacag tgttgaagaa
     8521 gcaattaaat tagctaaaga aacaagcatt gctaactttg acgcttctgt tgaagttgca
     8581 ttccgtttag gaattgatac acgtaaaaat gaccaacaaa tccgtggtgc agttgtatta
     8641 ccaaacggaa ctggtaaatc acaaagtgta ttagtattcg ctaaaggtga caaaattgct
     8701 gaagctgaag cagcaggtgc tgactatgta ggtgaagcag aatacgttca aaaaatccaa
     8761 caaggttggt tcgacttcga tgtagtagtt gctacaccag acatgatggg tgaagttggt
     8821 aaattaggtc gtgtattagg accaaaaggt ttaatgccaa accctaaaac tggaactgta
     8881 acaatggatg ttaaaaaagc tgttgaagaa atcaaagctg gtaaagtaga atatcgtgct
     8941 gaaaaagctg gtatcgtaca tgcatcaatt ggtaaagttt catttactga tgaacaatta
     9001 attgaaaact tcaatacttt acaagatgta ttagctaaag ctaaaccatc atctgctaaa
     9061 ggtacatact tcaaatctgt tgctgtaact acaacaatgg gtcctggagt taaaattgat
     9121 actgcaagtt tcaaataata aatgatataa acaattacag gctgaaagaa atatctttca
     9181 gtctgtaaaa atatattgac aataagtaat ttccaagtta tattacttat tgtgattatt
     9241 ttacctaaga cagtaggagt tatttataac ttaaaattta tcctgccgag gctaaaattg
     9301 acttgaacgt gatgatctat gatctttcaa gcactttttg ccgtgggtag aaagtgcttt
     9361 ttttattaat tttaaaaaaa gcaccaaaaa tttaaatgga ggtgtctgaa tgtctgctat
     9421 cattgaagct aaaaaacaac tagttgatga aattgctgag gtactatcaa attcagtttc
     9481 aacagtaatc gttgactatc gtggattaac agtagctgaa gttactgact tacgttcaca
     9541 attacgtgaa gctggtgttg agtataaagt atacaaaaac actatggtac gtcgtgcagc
     9601 tgaaaaagct ggtatcgaag gcttagatga attcttaaca ggtcctactg ctattgcaac
     9661 ttcaagtgaa gatgctgtag ctgcagcgaa agtaatttct ggatttgcta aagatcatga
     9721 agcattagaa attaaatcag gcgttatgga aggcaatgtt attacagcag aagaagttaa
     9781 aactgttggt tcattacctt cacacgatgg tcttgtatct atgcttttat cagtattaca
     9841 agctcctgta cgcaacttcg cttatgcggt taaagctatt ggagaacaaa aagaagaaaa
     9901 cgctgaataa tttttagcgt aaaaaaatta aaaataatgg aggaattata aaatggctaa
     9961 tcatgaacaa atcattgaag cgattaaaga aatgtcagta ttagaattaa acgacttagt
    10021 aaaagcaatt gaagaagaat ttggtgtaac tgcagctgct ccagtagcag tagcaggtgc
    10081 agctggtggc gctgacgctg cagcagaaaa aactgaattt gacgttgagt taacttcagc
    10141 tggttcatct aaaatcaaag ttgttaaagc tgttaaagaa gcaactggtt taggattaaa
    10201 agatgctaaa gaattagtag acggagctcc taaagtaatc aaagaagctt tacctaaaga
    10261 agaagctgaa aaacttaaag aacaattaga agaagttgga gctactgtag aattaaaata
    10321 attcaagtat cttaaactt
//
LOCUS       DAHHSC010000082        10314 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.083, whole genome shotgun sequence.
ACCESSION   DAHHSC010000082 DAHHSC010000000
VERSION     DAHHSC010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 10314)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10314)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10314
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.083"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            complement(206..1153)
                     /locus_tag="NBI57_001529"
     CDS             complement(206..1153)
                     /locus_tag="NBI57_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HCD1822471.1"
                     /translation="MRLQKAPLVTSGLVLGLLGLGNLLKDLSLTLNAVCGIFAFLIWI
                     HLLCTMIKYFNNVKEQLNSPLVSSVFTTFFMSGFLGTTYLNTFFSNITFINSLITPIW
                     ILCLVGIMTHMIIFSIKYLKDFSLENVYPSWTVLFIGIAIAGLTAPVSGCFFIGQLTV
                     IYGFVATCIVLPIVFKRLKAFPLQTSIKPNTSTICAPFSLVAAAYVIAFPKANAFIVI
                     IFLILAQIFYFYIIIQLPKLLKEPFSPVFSAFTFPLVISATALKNSLPVLMFPDIWKG
                     LLFIEVLLATVIVLRVFIGYLHFFLKKENQDKFLRNASQ"
     gene            1402..1590
                     /locus_tag="NBI57_001530"
     CDS             1402..1590
                     /locus_tag="NBI57_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HCD1822472.1"
                     /translation="MRNQIQKLLDSDLSSLHISKQTGVPQSTIHRMRKKERSLDNMSL
                     KNAELLYKFANSIFSNEN"
     gene            complement(2058..2131)
                     /locus_tag="NBI57_001531"
     tRNA            complement(2058..2131)
                     /locus_tag="NBI57_001531"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2095..2097),aa:Arg,seq:tct)
     gene            2495..2629
                     /locus_tag="NBI57_001532"
     CDS             2495..2629
                     /locus_tag="NBI57_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-class phenol-soluble modulin"
                     /protein_id="HCD1822473.1"
                     /translation="MEGLFNAIKDTVTAAINNDGAKLGTSIVSIVENGVGLLGKLFGF
                     "
     gene            2686..2820
                     /locus_tag="NBI57_001533"
     CDS             2686..2820
                     /locus_tag="NBI57_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-class phenol-soluble modulin"
                     /protein_id="HCD1822474.1"
                     /translation="MTGLAEAIANTVQAAQQHDSVKLGTSIVDIVANGVGLLGKLFGF
                     "
     gene            2944..3639
                     /locus_tag="NBI57_001534"
     CDS             2944..3639
                     /locus_tag="NBI57_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HCD1822475.1"
                     /translation="MGKLGYKNILIDFDDTIVDFYDAEEWAFHYMANVFNHKATKDDF
                     LTFKKINHQHWEAFQQNKLTKSEVLSERFVNYFKHHQMEVDGHRADVLFRNGLAEAKV
                     KYFDQTLETIVELSKRHDLYIVTNGVTETQKRRLNQTPLHKYIKKIFISEETGYQKPN
                     PEFFNYVFNDIGEDERQHSIIVGDSLTSDILGGINAGIATCWFNFRGFDHNPGIIPDY
                     EINSWKQLNDIVR"
     gene            complement(3748..4188)
                     /locus_tag="NBI57_001535"
     CDS             complement(3748..4188)
                     /locus_tag="NBI57_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCD1822476.1"
                     /translation="MSEIKRLEINYKTDELFENFRAFGNKDLYMVNELNGQMIDASSD
                     SPFYGIFVGDQLGARMALLKKGDVEEIYFPDFEDYILLWKLEVLPKYQNRGYASELID
                     FAKSFNMPIKAIGRNDSKDFFLHHGFTDVEAKNIEGHDVLLWKP"
     gene            4383..5996
                     /gene="bshC"
                     /locus_tag="NBI57_001536"
     CDS             4383..5996
                     /gene="bshC"
                     /locus_tag="NBI57_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000340457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacillithiol biosynthesis cysteine-adding enzyme
                     BshC"
                     /protein_id="HCD1822477.1"
                     /translation="MDCKVVSLNEKDQFIPKIKSSDPVITGLFQYDAAQQTSFEKRMS
                     KENNGREAALANVIREYMSDLKLSNEQELNIQHLANGSKVVIGGQQAGLFGGPLYTFH
                     KIFSIITLSKELTDTHKQQVVPVFWIAGEDHDFDEVNHTFVYNENHGSLHKVKYHTME
                     MPETTVSRYYPDKAELKQTLKTMFIHMKETVHTQGLLEICDRIIDQYDSWTDMFKALL
                     HETFKAYGVLFIDAQFEPLRKMEAPMFKKILKKHQLLDDAFRATQQRTQNQGLNAMIQ
                     TDTNVHLFLHDENMRQLVSYDGKHFKLNKTDKTYIKEEIINIAENQPELFSNNVVTRP
                     LMEEWLFNTVAFVGGPSEIKYWAELKDVFELFDVEMPIVMPRLRITYLNDRIEKLLSK
                     YNIPLEKVLVDGVEGERSKFIREQASHQFIEKVEGMIEQQRRLNKDLLDEVAGNQNNI
                     NLVNKNNEIHIQQYDYLLKRYLLNIERENDISMKQFREIQETLHPMGGLQERIWNPLQ
                     ILNDFGTDVFKPSTYPPLSYTFDHIIIKP"
     gene            6140..6571
                     /gene="mraZ"
                     /locus_tag="NBI57_001537"
     CDS             6140..6571
                     /gene="mraZ"
                     /locus_tag="NBI57_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015900043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HCD1822478.1"
                     /translation="MFMGEYDHQLDTKGRMIIPSKFRYDLNERFIITRGLDKCLFGYT
                     LDEWQQIEEKMKTLPMTKKDARKFMRMFFSGAVEVELDKQGRINIPQNLRKYANLTKE
                     CTVIGVSNRIEIWDRETWNDFYEESEESFEDIAEDLIDFDF"
     gene            6587..7522
                     /gene="rsmH"
                     /locus_tag="NBI57_001538"
     CDS             6587..7522
                     /gene="rsmH"
                     /locus_tag="NBI57_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017724209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HCD1822479.1"
                     /translation="MFHHISVMLNETIDYLNVKENGVYIDCTLGGAGHALYLLNQLND
                     DGRLIAIDQDQTAIDNAKEVLKDHLHKVTFVHSNFRELTQILKDLNIEKVDGIYYDLG
                     VSSPQLDIPERGFSYHHDATLDMRMDQTQELTAYEIVNNWSYEALVKIFYRYGEEKFS
                     KQIARRIEAHREQQPITTTLELVDIIKEGIPAKARRKGGHPAKRVFQALRIAVNDELS
                     AFEDSIEQAIELVKVDGRISVITFHSLEDRLCKQVFQEYEKGPEVPRGLPVIPEAYTP
                     KLKRVNRKPITATEEDLDDNNRARSAKLRVAEILK"
     gene            7536..7937
                     /gene="ftsL"
                     /locus_tag="NBI57_001539"
     CDS             7536..7937
                     /gene="ftsL"
                     /locus_tag="NBI57_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HCD1822480.1"
                     /translation="MAVEKVYQPYDEQVYNSIPKQQPQTKPEKKTVSRKVVVQLTKFE
                     KVLYITLITVIAMLSIYMLSLKMDAYDTRGKIADLDYKIDKQSSENSALQSEIKKNSS
                     YERIYEKAKKQGMSLENDNVKVVRSNGEAKN"
     gene            7918..10152
                     /locus_tag="NBI57_001540"
     CDS             7918..10152
                     /locus_tag="NBI57_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HCD1822481.1"
                     /translation="MAKQKIKIKKNKIGAVLLVGLFGLLFFILVLRISYIMITGHSNG
                     QDLVMKANEKYLVKNAQQPERGKIYDRNGKVLAEDVERYKLVAVIDKKASANSKKPRH
                     VVDKKETAKKLSTVIDMKPEEIEKRLSQKKAFQIEFGRKGTNLTYQDKLKIEKMNLPG
                     ISLLPETERFYPNGNFASHLIGRAQKNPDTGELKGALGVEKIFDSYLSGSKGSLRYIH
                     DIWGYIAPNTKKEKQPKRGDDVHLTIDSNIQVFVEEALDGMVERYQPKDLFAVVMDAK
                     TGEILAYSQRPTFNPETGKDFGKKWANDLYQNTYEPGSTFKSYGLAAAIQEGAFDPDK
                     KYKSGHRDIMGSRISDWNRVGWGEIPMSLGFTYSSNTLMMHLQDLVGADKMKSWYERF
                     GFGKSTKGMFDGEAPGQIGWSNELQQKTSSFGQSTTVTPVQMLQAQSAFFNDGNMLKP
                     WFVNSVENPVSKRQFYKGQKQIAGKPITKDTAEKVEKQLDLVVNSKKSHAANYRIDGY
                     EVEGKTGTAQVAAPNGGGYVKGPNPYFVSFMGDAPKKNPKVIVYAGMSLAQKNDQEAY
                     ELGVSKAFKPIMENTLKYLNVGKSKDDTSNAEYSKVPDVEGQDKQKAIDNVSAKSLEP
                     VTIGSGTQIKAQSIKAGNKVLPHSKVLLLTDGDLTMPDMSGWTKEDVIAFENLTNIKV
                     NLKGSGFVSHQSISKGQKLTEKDKIDVEFSSENVDSNSTNNSDSNSDDKKKSDSKTDK
                     DKSD"
BASE COUNT         3861 a         1426 c         1918 g         3109 t
ORIGIN      
        1 ctgcttatat attatttaaa taaaggttag agtgaaacgt gtttatgaac tagacgttct
       61 agtatagtta ctacagcttt agtcagaagg tatcattgat aagatcatat taaatcaaag
      121 aggcattgat atacactaaa aagaggcaag attacctgcc tcttttttag ttattaaata
      181 tgcgtgttaa ttcttggtaa tagtgttact gagacgcatt acgaagaaat ttatcttgat
      241 tttccttttt taaaaagaag tgaagatatc ctataaagac tctaagtact attacagtgg
      301 ctaataacac ttcgataaac aaaagacctt tccaaatatc tggaaacata agtacaggca
      361 aactgttctt taaagcagtt gctgagatta ctaaagggaa tgtgaaagct gaaaatacgg
      421 gcgaaaaagg ttcttttagt aatttaggca attgtataat gatataaaaa taaaatattt
      481 gagctaatat taaaaatata attacgataa aagcattcgc cttaggaaag gctataacat
      541 atgctgcagc gactaaagaa aatggtgcac aaattgtcga tgtgttcggt ttgattgacg
      601 tctgcaatgg aaatgctttt aatcgcttga aaacaatagg taagacaata caagtagcta
      661 caaagccata tattactgtt aattgaccta tgaaaaaaca tccgctaacg ggtgccgtca
      721 atcctgcgat agcaatacca ataaaaagta cagtccacga aggataaaca ttttcaagtg
      781 aaaaatcttt taaatatttt attgaaaaaa taatcatatg cgtcataatt cccacaaggc
      841 ataaaatcca aataggcgtt attaagctat tgataaaagt tatgttacta aaaaatgtat
      901 ttaaataagt agtacctaaa aagccagaca tgaaaaatgt tgtgaacact gatgaaacta
      961 gaggactgtt taattgttct ttcacattat taaaatattt gatcatagta cataaaaggt
     1021 gaatccaaat caagaaagca aagattccgc aaacagcgtt taaagtaaga gataagtctt
     1081 ttaatagatt acccaggcct aataatccta agactagtcc tgacgttact agaggtgctt
     1141 tttgaagtct catgatttac aacctttctt atgtgatttt cttcactaat tatatcatga
     1201 tagctatggc caattaataa gaagagtgtg tactattacg ttattagagt atgtatattg
     1261 gattgataga cacataattg acatttaaat ctcaaaatta atgatatttg aggtatgctt
     1321 tgcaacctaa atattggaat atgtggaaaa gtaattattt aatttaaatg atgattgaag
     1381 atttacaagg gggtgtacaa aatgagaaat caaattcaaa aactattaga cagtgatttg
     1441 agcagtttac atatatcgaa acaaacagga gttccacaaa gcacaataca cagaatgaga
     1501 aaaaaagaaa gatcattaga caatatgtca ttgaaaaacg ctgaactact ttataaattt
     1561 gccaatagta tatttagcaa tgaaaattaa gaagaataat tagtgagtta aataattaaa
     1621 aaaccacgtc tcgatggacg tggttatttt tttaaatgta tttaattata ggatttcggg
     1681 cccctgaaaa gtccctaaaa ttttgaatag tatcaaaatc tataggaata atataataat
     1741 aaaaatctct acgcatgcaa atggtgagtg ttaaaaatct tggtgtagtg ttggtgtagt
     1801 tcaggtgcag tatagatgaa atttgagcac aaaattactt aattaaaaat gtataaacaa
     1861 gtgtcagacg cgtgtcaatt gtgtgtcaaa aaattctatg acgaataagc atgcttgttt
     1921 taattactgt attttcgggg cttatgcggg ggacgttttg gtgacgcatc atactatatt
     1981 actgaaattc aaaacaaaaa gagccccgta atcacggaac tcttttgttt ggtaatgcgt
     2041 ataaaaatac ctataaacgt cctgggaggg attcgaaccc ccgaccgatg gcttagaagg
     2101 ccattgctct atccagctga gctaccagga cacgtttaac aacacaagaa ttattatatc
     2161 taaatgaact taaattagca ataccttgta aataaaaaat gtttatattt ttcactatta
     2221 tagagctatt tatctaaaaa ggttcaataa gacttaaatg cgaattcagg caacttaatt
     2281 gtgttaaata cagttttgaa tgcctaactg tatttctttt ctctttaaaa tacagttaag
     2341 tacattataa gatgttgtgc ggataaacaa actaattgca tcaaatttat tttaaaataa
     2401 caacaacaaa acgttaagcg aataacattt cggtgattta aaagctacgc acgtttttgt
     2461 tatcttcaaa tttaaatttt aaggagtgtt ttcaatggaa ggtttattta acgcaattaa
     2521 agataccgta actgcagcaa ttaataatga tggcgcaaaa ttgggcacaa gcattgtgag
     2581 catcgttgaa aatggcgtag gtttattagg taaattattc ggattctaat ttcaatatgt
     2641 tatgtaagta atcagtatta tttcaaaggt gagggagaga tttaaatgac tggactagca
     2701 gaagcaatcg caaatactgt gcaagctgca caacaacatg atagtgtgaa attaggcaca
     2761 agtatcgtag acatcgttgc taacggtgtg ggtttactag gtaaattatt tggattctaa
     2821 tataataact aatattcttt aaaataaact gggtgagcat actttaatgt tatgcactca
     2881 gtttatttta tttgcagaaa tttgagcctc tgttaagatt tagatacata gacaatatag
     2941 gagatgggga aattgggata taaaaatatt ttgatagact ttgatgatac aattgttgat
     3001 ttttatgatg cagaagaatg ggcgtttcac tatatggcga atgtttttaa tcataaagca
     3061 acaaaggatg attttttaac atttaaaaaa atcaatcacc aacattggga agcttttcaa
     3121 caaaataaat taacgaagtc tgaagtatta tcagaacgat ttgtgaatta cttcaaacat
     3181 catcaaatgg aagttgatgg gcatcgtgca gatgtgttat ttagaaatgg attagcagaa
     3241 gctaaagtta aatactttga tcaaacatta gaaacaattg tcgaattatc gaaaagacat
     3301 gatttatata ttgttactaa tggtgtaacc gaaacgcaaa agagaaggtt aaatcagacg
     3361 ccgttgcata aatatattaa aaagatattt atatctgagg aaacaggata tcaaaaacct
     3421 aatccggaat tttttaatta tgtttttaat gatattggtg aggatgaaag acagcactcg
     3481 attatagttg gagattcttt aacatctgac attctaggtg gaatcaatgc gggtatagct
     3541 acttgctggt ttaattttag aggatttgat cataatccag gaattatacc tgattatgaa
     3601 attaattcat ggaaacaact aaatgatatt gtacgttaag acaaataatt tgaaaatgat
     3661 ttacttatat tagcttaaga aagtaattgt aaaaacgaca ctacgaaaag atgcattttc
     3721 atgctcgtag tgtcgaatat tattatctta tggtttccac aataagacat catgtccctc
     3781 tatattttta gcttccacat ctgtaaatcc atgatgtaaa aagaaatcct tagaatcatt
     3841 tctgccaatg gctttaattg gcatattgaa actctttgca aaatcaatca attctgaagc
     3901 gtaccctctg ttttgatatt ttggtaatac ttctaacttc cataataata tataatcttc
     3961 aaaatctggg aagtagattt cttcgacatc accttttttt agtaatgcca ttctagctcc
     4021 taattgatct ccgacaaata tgccataaaa tggtgaatct gaacttgcat caatcatttg
     4081 accgtttaac tcattgacca tgtataagtc tttgttgcca aacgctctaa agttttcgaa
     4141 taattcgtca gttttgtaat taatttcaag acgtttgatt tcactcatat ttatctaccc
     4201 cttatttgtt tactactatt attatacatt aaaccacttg tgtttttcat tgatttgaaa
     4261 atgtaaaaca atgaacatta taaattttcc atgattaaat tgattttcaa gccgtaaaat
     4321 tgtcaatcac cggtatagac tttatactat agtttgtaat cgcgtactta aggagaaaaa
     4381 acatggactg taaagtagtt agtttaaatg aaaaagatca gtttatacca aaaataaaga
     4441 gcagtgaccc tgtaataaca ggattatttc aatatgatgc agctcaacaa actagttttg
     4501 aaaaaaggat gtctaaagaa aataatggaa gagaagcggc attagcgaat gttattcgtg
     4561 aatatatgag tgatttaaag ctttcaaatg aacaagaatt aaacatacaa catttagcta
     4621 atggttcaaa agttgtgatt ggtggacaac aagcagggct tttcggggga ccattgtata
     4681 cattccataa aatattttca atcattactt tatctaagga attaacggat acacataagc
     4741 aacaagtagt accagttttt tggattgcag gagaagatca tgatttcgat gaagtgaatc
     4801 atacatttgt ttataacgaa aatcatgggt cgctgcataa ggttaaatat catacaatgg
     4861 agatgccaga gacgacagtc tctagatatt atcctgataa ggctgagttg aaacaaactt
     4921 taaaaacgat gttcattcat atgaaagaaa ctgttcatac acaaggtcta ctggagattt
     4981 gtgacagaat tattgaccaa tatgactcgt ggactgatat gtttaaagca ctactgcatg
     5041 aaacatttaa agcatatggc gttctattta tagatgcgca gtttgagccg ttaagaaaaa
     5101 tggaagcgcc tatgtttaaa aagattttga aaaaacatca gttgcttgat gatgctttta
     5161 gagcaacaca acaacgtact caaaatcaag gcttgaatgc gatgatacaa acagatacaa
     5221 atgttcattt attcttacat gatgaaaata tgcgccaatt agtttcgtat gatggtaagc
     5281 attttaaatt aaataaaaca gataagacat atataaagga agaaattata aatattgcgg
     5341 aaaatcaacc tgaattattt tctaataatg tagtgacaag accattaatg gaagaatggt
     5401 tatttaacac ggtggcattt gttggaggac cgagtgaaat taagtactgg gctgaactaa
     5461 aagatgtatt tgaactattt gatgttgaaa tgcctatcgt gatgccaagg cttagaatta
     5521 cttatttaaa tgaccgtata gaaaaattac tttcgaaata caatattcca ttagaaaaag
     5581 tgttagtcga tggtgttgaa ggagaaagaa gtaagtttat tagagaacaa gcatcacatc
     5641 aatttattga aaaggtagaa ggtatgattg aacaacagcg tcgtctaaac aaagacttat
     5701 tagatgaagt ggcggggaat caaaataata ttaaccttgt gaataaaaat aatgaaattc
     5761 atatacaaca gtatgattat ttgttaaaac gttatctttt aaacattgaa agagaaaacg
     5821 acatcagtat gaagcaattt agagaaattc aagaaacact ccatccaatg ggaggattac
     5881 aagaaagaat atggaatcca cttcaaattt tgaatgattt tgggacagat gtgttcaagc
     5941 cctccaccta tccaccactt tcttacactt ttgatcatat tattataaaa ccttaatata
     6001 ccaagggttt agcccgattt atcttaatga taaatcgggc atttttttgt tttttaaaat
     6061 aaatttcaca aattttgtat aaatagtggt ggatagtggg gagatgtggt aaattatata
     6121 taaggtgagg tgataaaaaa tgttcatggg agaatacgat catcaattag atacaaaagg
     6181 acgtatgatt ataccgtcca agtttcgtta tgacttaaat gagcgtttta ttatcacaag
     6241 aggccttgat aaatgtttat tcggttacac tctagacgaa tggcaacaga ttgaagagaa
     6301 aatgaaaacc ttacctatga caaaaaaaga cgcacgtaag tttatgcgta tgttcttctc
     6361 tggtgctgtt gaagtagaac ttgataagca agggcgtatt aacatccctc aaaacttgag
     6421 gaaatacgct aatttaacta aagaatgtac agtaatcggt gtttcaaatc gtattgagat
     6481 ttgggataga gaaacttgga atgatttcta tgaagaatct gaagaaagtt tcgaagatat
     6541 tgctgaagat ttaatagatt ttgattttta aaatggagga attgaagtgt ttcatcatat
     6601 cagcgttatg ttaaacgaaa ccattgatta tttaaatgta aaagaaaatg gtgtgtacat
     6661 tgactgtacg ctaggtggag cgggacatgc cctttattta ctaaatcaat taaatgacga
     6721 cggaagatta atagcaatcg atcaagacca aactgcaatt gataatgcta aagaggtatt
     6781 aaaggatcat ttgcataagg tgacttttgt tcatagcaac ttccgtgaat taactcaaat
     6841 attaaaagac ttaaacattg aaaaagtaga tggaatttat tacgacttgg gtgtttcaag
     6901 cccacaactc gacattccag aacgaggatt cagttatcac catgacgcaa cattagacat
     6961 gcgtatggac caaacacaag aactaacagc atatgaaatt gttaacaatt ggtcatatga
     7021 agcgttagtg aagatttttt atcgttatgg cgaggagaaa ttttcaaaac agatagctcg
     7081 aagaatcgaa gcacatcgtg aacaacaacc aataacaaca acattagaat tagttgacat
     7141 tataaaagaa ggtattcctg caaaagcaag aagaaaaggc ggacatcctg caaaacgagt
     7201 atttcaagca ctacgaattg cagtaaacga tgaattgtca gcttttgaag attcaataga
     7261 acaagcgatt gaattagtga aagtagacgg caggatttcg gtaatcactt tccattcttt
     7321 agaagatcgt ttatgtaaac aggtgttcca agaatatgaa aaaggtccag aggtaccaag
     7381 aggattgcca gttataccag aagcatatac acctaagtta aagcgtgtta atcgtaaacc
     7441 gattaccgct acagaagaag atttagatga caataacaga gcacgaagcg cgaaattacg
     7501 tgtagctgaa atacttaaat aaggagcaat ttataatggc tgtagaaaaa gtgtaccaac
     7561 catatgacga acaagtttat aatagtatac cgaagcaaca accacaaact aagcccgaaa
     7621 agaagactgt ttcgagaaaa gtggttgtac aattaactaa atttgaaaaa gttttataca
     7681 taactttgat tactgtaatt gctatgttaa gtatttatat gctatcttta aaaatggatg
     7741 cgtatgatac gcgaggaaag attgcagatt tagattataa aatagataaa caatcaagtg
     7801 aaaacagtgc tttacaatct gaaatcaaaa agaattcttc ttatgaacgc atatacgaaa
     7861 aggctaagaa acaggggatg agccttgaga acgataatgt aaaggtagtg cgtagtaatg
     7921 gcgaagcaaa aaattaaaat taaaaaaaat aaaatagggg cagtcctact tgttggttta
     7981 ttcggactgc tcttttttat attggtttta agaatttcat atatcatgat tactggacat
     8041 tctaatggtc aagatttagt catgaaggca aatgaaaagt atttagttaa gaatgcacaa
     8101 caaccagaac gaggaaagat atatgatcgt aatggtaaag tgctagcaga agatgtagaa
     8161 agatataaac ttgttgcagt aatagataaa aaggcgagtg ccaattctaa aaaacctagg
     8221 catgtagttg ataaaaaaga gactgcaaag aaattatcta cagtcattga tatgaagcca
     8281 gaggaaattg aaaagagact tagtcaaaag aaagctttcc aaattgaatt tggacgcaaa
     8341 ggaacaaatt taacgtatca ggacaaattg aaaatagaga aaatgaattt gcctggtatt
     8401 tctttattgc ctgaaacaga acgcttttat ccaaatggca attttgcatc acacttaatt
     8461 ggtagagctc agaaaaatcc ggatactggt gaacttaaag gtgcacttgg agttgaaaag
     8521 atttttgata gttatttaag tggatctaaa ggatcattga gatatattca tgatatttgg
     8581 ggatatatcg caccaaatac taaaaaagag aagcagccta aacgtggtga tgatgtccat
     8641 ttaacaatcg attcaaatat tcaagtattt gttgaagaag ctttagatgg catggttgaa
     8701 agataccagc cgaaagattt atttgcggtt gtcatggatg ccaaaactgg agaaatttta
     8761 gcatacagtc agcgaccaac atttaatcct gaaactggta aagactttgg taaaaagtgg
     8821 gcaaatgacc tttatcaaaa cacatacgag cctggatcaa catttaaatc atatgggtta
     8881 gcagctgcta ttcaagaagg tgcttttgat cctgataaga aatataaatc tggacataga
     8941 gatattatgg gttcacgtat ttcagactgg aatagagtcg gttggggtga aatcccaatg
     9001 tcactcggat ttacttattc atctaataca ttgatgatgc atttacaaga tttagttggt
     9061 gcagacaaaa tgaaatcttg gtatgaacga tttggatttg gaaaatcaac taaaggtatg
     9121 tttgatggag aagcacctgg tcaaattgga tggagtaatg agttgcaaca aaaaacgtca
     9181 tcatttggtc aatcgacaac agtaacacct gttcaaatgt tacaggcgca atcagcgttc
     9241 tttaatgatg gtaatatgtt aaaaccatgg tttgtgaata gcgttgaaaa tcctgttagt
     9301 aaaagacaat tttataaagg tcaaaaacaa atcgcaggca aaccaataac aaaagatact
     9361 gctgaaaaag ttgaaaagca attggattta gttgtgaata gtaagaagag tcacgctgca
     9421 aactatcgta ttgatggtta tgaggtcgaa ggtaagactg gtacagcaca agtcgctgca
     9481 cctaatggtg gtggatacgt taaaggtcca aacccatatt ttgtaagttt tatgggtgac
     9541 gcaccgaaga aaaatcctaa agttattgta tacgctggta tgagcttggc acaaaaaaat
     9601 gaccaagaag cttatgaatt aggtgttagt aaagcgttta aaccaataat ggaaaatact
     9661 ttgaaatatt taaatgtagg taaatcaaaa gatgacacat ctaatgcaga gtatagtaaa
     9721 gtgccagatg ttgaaggtca agacaaacaa aaagctattg ataatgtgag tgcaaaatca
     9781 ttagaaccag ttactatcgg ttctggcaca caaataaaag cacaatctat aaaagcaggg
     9841 aataaagtct tacctcatag taaagtactg ttattaacag acggagactt aactatgcct
     9901 gacatgtcag gatggacgaa agaagatgtc attgcttttg aaaacctaac aaatattaaa
     9961 gtaaatttaa aaggtagcgg ttttgtgtcc caccaatcaa ttagtaaggg acaaaaactt
    10021 actgaaaaag ataaaataga cgtagaattt tcatcagaga atgtagacag caattcgacg
    10081 aataattctg attcaaattc agatgataag aagaaatctg acagtaaaac tgacaaggat
    10141 aagtcggact aacaagaatg gctacattaa ataagggtgt attgtcacta tttgttttta
    10201 ctaaagtaat gctgtgatag aaaaaaacat cttcatttaa tctttataaa taagtgttga
    10261 tagagaattt taaggtagac gaggtcagat taacttgttt gttataggca aact
//
LOCUS       DAHHSC010000083        10293 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.084, whole genome shotgun sequence.
ACCESSION   DAHHSC010000083 DAHHSC010000000
VERSION     DAHHSC010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 10293)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10293)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10293
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.084"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            complement(<1..125)
                     /locus_tag="NBI57_001541"
     CDS             complement(<1..125)
                     /locus_tag="NBI57_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000613248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP synthase"
                     /protein_id="HCD1822482.1"
                     /translation="MIPTLEEIIDKYGNLVDYLKFDVTVEVYEDLLLLRSLSDLN"
     gene            264..554
                     /locus_tag="NBI57_001542"
     CDS             264..554
                     /locus_tag="NBI57_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001036438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1822483.1"
                     /translation="MNKKSKQQEKLYNFIIAKSFQQPVGSTFTYGELRKKYNVVCSTN
                     DQREVGRRFAYWIKYTPGLPFKIVGTKNGSLLYQKIGINPCNNSTPSKGGDC"
     gene            554..2341
                     /locus_tag="NBI57_001543"
     CDS             554..2341
                     /locus_tag="NBI57_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001573627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF927 domain-containing protein"
                     /protein_id="HCD1822484.1"
                     /translation="MSYTLFEIGPFALDSFGWKETIPPKKDGDSEKVVRMASPIIVNA
                     RFSDPITGVEKLIITNNNGKKDIFESDILTTRNLPSLIKYGYSINEKYIRSLSYALQL
                     MRDRLPLSELYEGVGILETPFGYLISLDKVLKSIQFNQSSPSYPIVDSAYDLTPKGTF
                     DNWFNMYIDEVKGHLLLELAVIFGISALVTSFLKHKHEIEFAGILFSFTGQSSTGKST
                     AAALAVSVAGNPTKGNETLFRSWNATRNALEGYLSNNYGIPIVFDELSSTTLRDTTGL
                     LYSIAEGQGRQRSNVHGEVKTPKNWGTSVISTSEYSIFNDSAQNDGLRVRTIEINEQF
                     TTNATNADNIKKAVALNYGHVLPLVAKYLINREDEVIQWFYKEVDWFEAKLKDETNNT
                     GIRMFKRYAVITTSAKILGRVLSTDIDIANIRDYFIDYHTHTVSERSLADKAIDVIIQ
                     FVAQNRGKFSDEGALKNMFENYGLISLKDDHIEVKIIANVFKQMLNNHQFQDVNNVVN
                     ALRDKGFILADRGRQTTKRSVKDNSGKKQSLVFYHLKLDVEFASILGLTKDKSLLQNW
                     TPSNDNKAAKELFKSANEGIGPSGVHEDF"
     gene            2575..3924
                     /locus_tag="NBI57_001544"
     CDS             2575..3924
                     /locus_tag="NBI57_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000815654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCD1822485.1"
                     /translation="MKQVIGYLRQSTMKQQSLAAQKQAIEAIAEKHHIQHINFYSDKQ
                     SGRKDNRSGYRQITQLIQQGQCDILCCYRLNRLHRNLKNALKLIKLCQTYHVHILSVH
                     DGYFDMDQAFDRLKLNIFISLAELESDNIGEQVRNGLQEKAKQGRLITTHAPFGYEYH
                     NGTFIINQNESPTVKAVFNYYIKGHGYKKIAQLLEEDNTYINRQPYQVRNIIINPNYC
                     GRVNNQYGQFDNMFPSIVSTSIYEQAQRLRLQKQTKQTPSDNQLKQKIKCPCCNATLT
                     NMTIRKKNHTLRYYVCPKNMNASRFVCDFKGINAQTLEDKVLEVCRDFYQNQRIYTKI
                     KSAIDKRIKRQRNIEKHHTLTQEQLIEKLAQGIIDAETFREQTQSLRQQPQRTTSING
                     HQIQHIIQNIIQKRFTLNILYPYIETIHITKDKNLIGIYFKNEPLNIVNQTMQSSIA"
     gene            3946..5574
                     /locus_tag="NBI57_001545"
     CDS             3946..5574
                     /locus_tag="NBI57_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001186610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCD1822486.1"
                     /translation="MQQLKTKRVGIYVRVSTEMQSTEGYSIDGQINQIKEYCDFHHFE
                     VKDIYADRGISGKSMNRPELQRMLKDAKEGNIDCVMVYKTNRLARNTSDLLKIVEDLH
                     KQNVEFFSLSERMEVNTSSGKLMLQILASFSEFERNNIVENVFMGQTRRAQEGYYQGN
                     LPLGYDKIPDSKHELMINQHEANIVKYIFECYAKGHGYRKIANALNHKGYVTKKGKPF
                     SISSITYILANPFYIGKIQFAKYKDWSEKRRKGLNDKPVIAEGKHSPIINQDLWDKVQ
                     MRKKQVSQKPQVHGKGTNLLTGIIHCPQCGAPMAASNTTNTLKDGTKKRIRYYSCSNF
                     RNKGSKVCSANSVRADVIEDYVMKQILEIVKSDKVIQRVVTHVNQENQVDGAALHHDI
                     AYKQQQYDEVQIKINNLIKTIEDNPDLTSVIRPSIQKYEKQLNDITNQINQLKNQQNE
                     DKTLFDAKEISKLLQHIFHDIKHIEKSRLKALYLSVIDRIDIKKDGNHKKQFYVTLKL
                     NNEIIKQLFNNKQLDEVHLSTSSLFLPQTLYLTI"
     gene            6096..6446
                     /locus_tag="NBI57_001546"
     CDS             6096..6446
                     /locus_tag="NBI57_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002502216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1822487.1"
                     /translation="MKTITQELKQYITHLFQLSNNEAWECEALEEAAENILPERFIND
                     SPLVHLTLETYTYYNDELHELSIYPFLMYANNQLISVGYLDHFDMDFLYLTDTQNIII
                     DERHLLKQGGQDHE"
     gene            6533..6844
                     /locus_tag="NBI57_001547"
     CDS             6533..6844
                     /locus_tag="NBI57_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002505017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HCD1822488.1"
                     /translation="MKINRYITRGINESIPLDLQILLWHMVEKKDNQPHTDYLHIFKL
                     QEDENILSITHEQEQPTYKLEYHYTNYEKNQNALPKKVYVIREDDVDAFYYVMLLPEE
                     Y"
     gene            6859..7365
                     /locus_tag="NBI57_001548"
     CDS             6859..7365
                     /locus_tag="NBI57_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001092170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1643 domain-containing protein"
                     /protein_id="HCD1822489.1"
                     /translation="MNTIKSTIHTEAIFSDDEQHRYLLKKTWDEKKAACTVITMYPHL
                     DGVLSLDLTTVLILNQLANSERYGAVYLVNLFSNIRTPENLKHIKNPYDEHTDIHLMK
                     AISESDTVILAYGAYAKRPVVIDRVEQVMEMLKPHKKKVKKLINPVTNEIMHPLNPKA
                     RQKWILKS"
     gene            7417..9024
                     /locus_tag="NBI57_001549"
                     /pseudo
     CDS             7417..9024
                     /locus_tag="NBI57_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011274780.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182 family transposase"
     gene            complement(9015..9275)
                     /locus_tag="NBI57_001550"
     CDS             complement(9015..9275)
                     /locus_tag="NBI57_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001549961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3387 domain-containing protein"
                     /protein_id="HCD1822490.1"
                     /translation="MYVVYRASHETAKEALGDKELRAIAHELTKTVKDNMSVDWSKRD
                     SAKAKMRVQVRRLLKKYGYPPDLQKMAVEQVVEQAELMASQQ"
     gene            complement(9247..10233)
                     /locus_tag="NBI57_001551"
     CDS             complement(9247..10233)
                     /locus_tag="NBI57_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001549960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactam sensor/signal transducer MecR1"
                     /protein_id="HCD1822491.1"
                     /translation="MLSSFLMLSIISSLLTICVIFLVRMLYIKYTQNIMSHKIWLLVL
                     VSTLIPLIPFYKISNFTFSKDMMNRNVSDTTSSVSHMLDGQQSSVTKDLAINVNQFET
                     SNITYMILLIWVFGSLLCLFYMIKAFRQIDVIKSSSLESSYLNERLKVCQSKMQFYKK
                     HITISYSSNIDNPMVFGLVKSQIVLPTVVVETMNDKEIEYIILHELSHVKSHDLIFNQ
                     LYVVFKMIFWFNPALYISKTMMDNDCEKVCDRNVLKILNRHEHIRYGESILKCSILKS
                     QHINNVAAQYLLGFNSNIKERVKYIALYDSMPKPNRNKRIVAYIVCSISSFT"
BASE COUNT         3862 a         1767 c         1620 g         3044 t
ORIGIN      
        1 tcatttaaat cgcttaaact tcttaataac aacaaatctt cataaacctc aactgttaca
       61 tcaaatttca aataatcaac taagtttcca tacttatcaa taatttcttc tagcgtaggt
      121 atcaatatat accccccctc cccatttttt gttccgcgtc taatcataat gcttcattta
      181 tgcttattat agcaatcaat tgaagatagt ttaacctata tgatattgat tgactcctta
      241 aaaattattg gaggaatttt aaaatgaata agaaatctaa gcagcaagaa aagctttata
      301 attttataat tgctaaatct tttcaacaac cagttggaag tacgttcact tatggtgaat
      361 taagaaagaa atataatgtg gtttgtagca cgaatgatca acgtgaagta ggacgtcgtt
      421 ttgcttactg gattaagtac acgccaggat taccatttaa aattgtagga acaaaaaatg
      481 gcagtttatt atatcagaaa atagggataa acccatgcaa taatagcacg ccttcgaaag
      541 gaggtgattg ctaatgagtt acacattgtt tgaaattggc ccatttgctt tagattcctt
      601 cggttggaaa gaaacgatac cacctaaaaa agatggtgac agtgaaaaag tggttagaat
      661 ggctagccct attattgtta atgcaagatt ttctgatcca attactggtg ttgaaaagtt
      721 gattattact aacaacaatg gtaaaaaaga catttttgag tctgatatct taacaacacg
      781 aaacttacct tctttaatta aatatggcta cagtattaac gagaagtata ttagatcatt
      841 gagttatgcc ttgcaattga tgcgagatag attaccactt tctgaattat atgagggtgt
      901 aggtatacta gagacacctt ttggctactt gatatcgcta gataaagttc tcaaatctat
      961 tcaatttaat cagtcctcac cttcataccc tattgtagat agtgcttatg acttaactcc
     1021 taaaggtaca tttgataatt ggtttaacat gtatattgac gaggttaaag gtcatcttct
     1081 tttagaattg gcagtcatat ttgggatttc tgccttagtg acaagttttc tgaagcataa
     1141 acatgaaatc gaatttgcag gaattctctt tagttttaca ggtcaatcct caaccggtaa
     1201 gtcaaccgca gctgctttag ctgtatcagt cgctggtaac cctaccaaag gtaatgaaac
     1261 tctttttaga agttggaatg ccacacgaaa cgcacttgaa ggttatttga gtaacaatta
     1321 tggcattcct atagtatttg atgagctttc atctacgaca cttagagata ccacgggttt
     1381 gttatattcc atcgctgaag gtcaaggtcg tcaacgttca aacgttcatg gagaagttaa
     1441 aacgcctaag aattggggta cttctgtcat ttcaacgtca gaatacagta tttttaatga
     1501 ttcagctcaa aacgatggat tacgtgtaag aaccatcgag attaatgaac aattcactac
     1561 aaatgcgacc aatgctgaca atattaaaaa agctgtagca ctaaactatg gtcatgtttt
     1621 accgttagta gctaaatatc ttataaatcg tgaagatgaa gttattcaat ggttttacaa
     1681 ggaagtagat tggtttgaag ctaaattgaa agatgaaaca aacaatacag gtattcgtat
     1741 gtttaagcgt tatgcagtca ttacaacatc tgcgaaaata ttaggtcgtg ttttatctac
     1801 agatattgat atcgctaata taagagacta ctttattgat tatcatactc atacagtctc
     1861 tgaacgctca ttagctgata aagctattga tgtcatcatt caatttgtgg cacaaaacag
     1921 aggtaagttt tcagatgaag gtgctttgaa aaatatgttt gagaactatg gattaatctc
     1981 attaaaagat gaccatatcg aagtaaaaat aattgcgaac gtttttaaac agatgctaaa
     2041 caatcatcaa tttcaagatg ttaataatgt tgtcaacgcc ttaagagata aaggtttcat
     2101 attagctgac cgtggacgcc aaacaactaa aagaagtgtc aaagataata gcggtaaaaa
     2161 acaatcactt gtattttacc atctaaaatt agatgtagag tttgcatcta tcctcggatt
     2221 aactaaagat aagtcgttac ttcaaaattg gacaccgtca aatgataaca aagctgcgaa
     2281 agaacttttt aaatccgcta atgaagggat aggcccttca ggagttcacg aagatttcta
     2341 attgtcttta tcatacaact gtgaagcatc aaactgaata atcgacgtat tgaagataga
     2401 acaaataacg gagtgaaaaa catggatgaa cttatcgaga tattagccga tttggtaatt
     2461 gaagaaatca atcaaacaaa tgactaatat ggacgcccaa catctcattg atgttgggct
     2521 tatatatcaa ttcatccata aatatatatt atcactgtga aaggaggagc cgatatgaaa
     2581 caagtcatag gctatttacg tcaaagtacg atgaaacaac aatctcttgc agcacagaaa
     2641 caagctatcg aagcaatagc cgaaaaacat catattcaac atatcaactt ttatagcgac
     2701 aaacaatcag gtcgcaaaga taatcgtagt gggtatcgac aaataacaca attaattcaa
     2761 caaggacagt gtgacatatt atgctgttat cgtcttaata ggttgcatcg taatctgaaa
     2821 aatgcattaa aactcatcaa attatgtcaa acgtaccatg ttcatatctt aagcgtacat
     2881 gatggttact ttgatatgga tcaagctttc gaccgactca agcttaatat cttcatcagt
     2941 ttagccgaac ttgaatcgga taacattgga gaacaagtca gaaatgggct tcaagaaaaa
     3001 gcaaagcaag gtcgattgat tacaacacat gcaccttttg gttacgaata tcacaacgga
     3061 acattcatca tcaatcaaaa tgagtcacca acggtaaagg ctgtattcaa ttattacatt
     3121 aaaggtcatg gttataagaa aattgcacag ttattagaag aagataacac atatatcaat
     3181 cgacaaccct atcaagttcg taacattatc atcaatccta attattgtgg tcgtgtcaac
     3241 aatcaatatg gccaattcga caatatgttt ccttctattg tttccacaag tatatatgag
     3301 caagcgcaga gacttcgatt gcaaaaacaa accaaacaga caccttcgga taatcaactc
     3361 aaacaaaaaa tcaaatgccc atgttgtaat gcaacactta caaatatgac cattagaaaa
     3421 aagaatcata cattacgtta ctacgtctgt cctaaaaaca tgaatgcttc acgctttgtc
     3481 tgtgatttta aaggcatcaa tgcacaaaca cttgaagata aagtattaga agtgtgccga
     3541 gacttttatc aaaatcaacg catctacaca aaaattaaaa gtgcgattga caaacgcatc
     3601 aaaagacaaa gaaacataga aaaacatcac acattgactc aagaacaact gatagaaaag
     3661 ttggcacaag gcatcattga tgcagaaacg ttcagagagc aaacacaatc attacgtcaa
     3721 caaccgcaac gcactacatc tatcaatggg catcaaatac aacacatcat tcaaaatatt
     3781 attcaaaaac gtttcacgtt aaacatattg tacccctata ttgaaaccat tcacattacg
     3841 aaagataaaa atcttatagg aatctatttc aaaaatgaac cactcaatat tgtcaatcaa
     3901 accatgcaat catcgattgc ataaatgaag aaaggatgta acataatgca acaacttaaa
     3961 acaaaacgtg tcggtatcta tgtacgtgta tcaacagaaa tgcaaagcac agaaggttat
     4021 agtatcgacg gacaaatcaa tcaaatcaaa gaatactgtg acttccatca ttttgaagtt
     4081 aaagatatat acgctgaccg tggtatttca ggtaaatcta tgaacagacc tgagctccaa
     4141 cgtatgttga aagatgcaaa agaaggcaat atcgactgtg ttatggtata caaaacaaac
     4201 cgattagctc gtaatacatc tgatcttctc aaaattgtcg aagatttaca caaacaaaat
     4261 gtcgaatttt tcagtttgtc agagcgtatg gaagtcaata cttcttctgg taaactcatg
     4321 ttacagatac ttgcgagttt ctcagaattc gaacgtaata acattgtcga gaacgtattt
     4381 atgggtcaaa cgagacgtgc ccaagaaggc tattatcaag gcaatttacc actaggttat
     4441 gacaaaatac cagatagtaa acacgagcta atgattaacc aacatgaagc taatattgta
     4501 aaatatatat tcgagtgcta tgccaaagga catggctatc gtaaaattgc caatgcattg
     4561 aatcacaaag gatatgtcac taaaaagggg aaacctttca gtattagttc aatcacatac
     4621 atcttagcta accctttcta tatcggcaaa attcaatttg cgaaatacaa agattggagt
     4681 gaaaaacgtc gtaaagggct gaatgataaa ccagtgatag ctgaaggtaa gcattccccc
     4741 attattaatc aagatttatg ggataaagta caaatgcgta agaaacaagt cagtcaaaaa
     4801 ccccaagtcc atggcaaagg aacgaatctg cttacaggca ttattcactg tccccaatgt
     4861 ggcgcaccta tggcagcaag caataccacg aatactctta aagacgggac taagaaacgt
     4921 attcgttact attcatgtag taattttcgg aacaagggtt ccaaagtatg ttcggcaaac
     4981 agtgtaagag ctgatgtgat tgaagattat gtgatgaagc aaatacttga aatagtcaaa
     5041 agtgataaag tcattcaacg cgtagtaaca cacgtcaatc aagaaaatca agttgatggc
     5101 gctgcacttc atcacgatat tgcttataag caacaacaat atgatgaagt acaaatcaaa
     5161 ataaataact tgattaaaac catcgaggat aatccggact taacatcagt aatcagacca
     5221 agtattcaaa aatatgaaaa gcaactcaat gacattacga atcaaatcaa ccaactcaaa
     5281 aatcaacaaa atgaagataa gactttattt gatgccaaag aaatcagtaa actattacaa
     5341 cacatctttc atgatattaa gcacatagaa aaatctcgac tcaaagcatt gtatctatca
     5401 gtgattgatc gcattgatat taaaaaagat ggtaatcata aaaaacaatt ctatgtcaca
     5461 ctcaaactta ataacgaaat cattaaacaa cttttcaata ataaacaact cgacgaagtg
     5521 catctcagca cttcgtcttt atttttgccc caaacactat atcttactat ttagctaata
     5581 gaatcattgt tataacaata attttcacta tattctttcg cttattttat ctaactattt
     5641 tcaagaattg ctctttatca atttcaataa cacttatcta atgaaacagt tattgtcgta
     5701 attttttcat aactatacaa tgcttatatt gaaattgcta aagaacatcc cacaatgatt
     5761 ttttatcggg aaatgattgg gatttgcaga ttcgaagaaa cagaaggatt gtgcaagcac
     5821 aatcccaaaa tcccgttact ttttataaat aaataatata tataaggaag cagagacaca
     5881 accctatcat caatacttat aaataatgag ttgattgttt gtcaaaatag gcggaatttc
     5941 aggatttcct tggcatacca acgtttgtag cgggttttta tatggaattt tctcttattc
     6001 ccaccgggat tcgtaataaa tgacaaatca aatatatatt atctcaatga tgtatatgaa
     6061 ctacatcact attacactat ctaggaggca ataatatgaa aaccatcact caagaactca
     6121 aacaatatat aacccaccta ttccaactat ctaataacga agcttgggaa tgtgaagcgc
     6181 tggaggaagc agcagaaaat attctaccgg aacgttttat taatgattct ccactggtac
     6241 atctcacact cgagacttat acctactata atgatgaact acatgagcta agtatctatc
     6301 catttctaat gtatgccaat aaccaactca tcagtgtcgg atacttagac cattttgaca
     6361 tggactttct gtaccttaca gatactcaaa atatcattat tgatgaacgt catttactaa
     6421 aacaaggagg tcaggaccat gaataattgg atcatagttg ctcaattatc cgtcacagtt
     6481 atcaacgaaa tcatcgacat catgaaagaa aagcagaagg gagggaaata gcatgaaaat
     6541 caatcgatac atcacaagag gaattaatga aagtattcca ctagaccttc aaatcttact
     6601 ttggcacatg gtagaaaaaa aagataacca gcctcataca gattacctac acatttttaa
     6661 attacaagaa gatgagaata tactctcaat cacacatgaa caagaacaac ccacatacaa
     6721 attggaatat cactatacaa actatgaaaa aaatcaaaat gcattaccta agaaagtcta
     6781 cgtcattcgt gaagatgacg tggacgcttt ttattatgtg atgcttttac cagaagaata
     6841 ctaaaggaag tgacgatgat gaatacaatc aaaagtacga tacacacaga agccatattt
     6901 agcgatgatg aacagcaccg ctatttactc aagaaaacat gggatgaaaa gaaagctgca
     6961 tgcacagtga taacgatgta tcctcattta gacggtgtat tatcactcga tctcacaact
     7021 gttcttatcc tcaaccaatt agccaattca gaacgatacg gtgctgtata tcttgtaaat
     7081 ctattctcta atattagaac acccgaaaac ctcaaacata tcaaaaatcc atacgatgag
     7141 cacactgata ttcatttgat gaaagcgatt agtgaaagtg acacagtgat tcttgcttat
     7201 ggtgcctatg cgaagcgacc agttgttatc gaccgtgtcg aacaagtgat ggaaatgtta
     7261 aaacctcata aaaagaaagt aaaaaagctc atcaatccag taacaaatga aattatgcat
     7321 ccactcaacc ctaaggcacg tcaaaaatgg attttgaaat catagcaaat acatggaggt
     7381 caatatggaa acaaaacaaa tcaacatcgt tttactgtaa atgatattgt tgaaacaatt
     7441 cctgacaatg aattcgacga attcagacat caccgtggtg caacttcgta ccatcctaaa
     7501 atgatgttaa aagtgattct atatgcctac acacaatctg tattctcagg tcgtaaaata
     7561 gaaaaaatgc ttaatgatag catccgaatg atgtggctat cacaaaatca aaaaccttct
     7621 tataaaacaa ttaatcaatt tagagtaaat ccaaaagtag atgctttatt agaatcttta
     7681 tttattcaat tttacagtca gtgtgtaaaa caaaatctta tagatgataa agctattttt
     7741 attgatggta caaaaattga agcaaatgcc aatcgatata catttgtatg gaaaaagagt
     7801 attcaaaacc atgaatcaaa gatgaatgag gattctaaag ccctctacca tgaattggta
     7861 accaataaaa tcataccgga aattaaagaa gatcatgata atgaattaac aaaagaagaa
     7921 atagatttga ttggtagtca cttagataaa gaaatcgaag atttaaacca acatatcaac
     7981 aatgaaaaat gtactaaaac aagaaaacaa atacgtctca aaagaactaa aatcaaaaaa
     8041 tacaaaaagc aaatcaatga ttattttgag cgaaagtatc gatacgaatt tcaaaaatct
     8101 attttaaagg atagaaatag ttattctaag acagattatg atgcgacatt tatgagaatg
     8161 aaagaagatc acatgaaaaa tggacaactt aagccagggt ataatttaca aatagcaaca
     8221 aattcccaat ttgttttatc ttataatgtg tatcaaaatc caacggatac tagaacgatg
     8281 attccatttt taaattcaat tcaagagacc tacggtcatt tacctgaata tattgtagct
     8341 gatgcaggtt atggtagtga atcaaattat aaggcaatta tagatgactt taatcgaacg
     8401 ccactcataa catatggaat gtttataaaa gataaaacta aaaaatataa aagtgacatc
     8461 tttaatactc aaaattggaa ctatgacgaa attaatgacg aattcatttg tccgaataat
     8521 aaacggctag gttttaaaag atatgcctat cgtcatgata agtatggtta taagcgagac
     8581 ttcaaattat atgaatgtga tgattgttca gaatgtcctc tgaaaaatca atgtatgaac
     8641 ttcaattcaa aaacaaacaa aaaaataatg aagaattata actgggaata ttttaaatcc
     8701 caaattaaca aaaagctttc agaaccagaa acaaaaaata tctacagtca aagaaaaatt
     8761 gatgtggaac ctgtttttgg atttatgaag gctattttgg gtttcactcg gatgtctgtc
     8821 cgaggactca ataaagtcaa aagagaactt ggttttgtat taatggcact taatataaga
     8881 aaagtagtag ctcaacgagc tgaaaataat caaaaaattt ataaaaaaga caatttctat
     8941 attatttcaa tagaaattgt cttttttcac ttatccaaga actttatgtc ccggactcat
     9001 tatgatttat ttttttattg ctgacttgcc attaattctg cttgctctac aacttgttcc
     9061 acagccattt tttgaagatc tggtggatag ccatatttct ttaataggcg tctaacttga
     9121 actctcattt tagctttagc actgtctcgt ttagaccaat caacactcat gttatcctta
     9181 actgttttag ttaactcatg tgcaatggct cttaactctt tatcgcccaa agcttcttta
     9241 gctgtttcat gtgaagctcg atatactaca tacaatatac gcaacaatac gcttgtttcg
     9301 attaggttta ggcattgaat cataaagtgc aatatactta acacgttctt taatatttga
     9361 attaaaacct agtaaatatt gtgctgccac attatttatg tgctgagatt ttaaaataga
     9421 gcattttaat atcgattcac cataacgtat atgttcatgg cgattcaaaa tttttaaaac
     9481 gtttctatca catacttttt cacagtcatt gtccatcatt gttttactta tatatagtgc
     9541 aggattaaac cagaatatca ttttaaaaac aacataaagc tggttgaata ttaagtcatg
     9601 acttttcaca tgtgatagtt catgtagaat aatatattca atttctttgt cattcatggt
     9661 ttcgactacg acagttggta gtacaatttg ggatttcact aaaccaaata ccatcggatt
     9721 atcaatgttt gaactataac taattgttat atgctttttg tagaactgca tcttactttg
     9781 acatacttta agtcgttcat taagatatga cgattccaat gacgaacttt taataacatc
     9841 aatttgtcgg aatgccttaa tcatataaaa taagcacaac aaactaccaa atacccatat
     9901 caaaagaatc atatacgtta tatttgaggt ctcaaactga ttaacattaa ttgctaagtc
     9961 tttcgtaaca gatgattgtt gaccatctaa catatgacta accgaagaag tcgtgtcaga
    10021 tacatttcga ttcatcatat cttttgaaaa tgtaaaattc gatattttgt aaaatggtat
    10081 taatggaatt aacgtggaga cgagcactaa taaccaaatc ttatgtgaca taatattttg
    10141 agtatatttt atatagagca ttctcactaa aaaaattaca catatcgtga gcaatgaact
    10201 gattatactt aacattaaaa aagatgataa caccttctac acctccatat cacaaaaatt
    10261 ataacattat tttgacataa atactacatt tgt
//
LOCUS       DAHHSC010000084        10271 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.085, whole genome shotgun sequence.
ACCESSION   DAHHSC010000084 DAHHSC010000000
VERSION     DAHHSC010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 10271)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10271)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10271
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.085"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            60..986
                     /locus_tag="NBI57_001552"
     CDS             60..986
                     /locus_tag="NBI57_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCD1822492.1"
                     /translation="MGKYIFKRFIYMLISLFIIITITFFLMKLMPGSPFNDAKLNAEQ
                     KEILNEKYGLNDPVATQYLHYLKNVVTGDFGNSFQYHNQPVWDLIKPRLLPSFEMGLT
                     AMFIGVILGLILGVAAATKQNSWVDYTTTVISVIAVSVPSFVLAVLLQYVFAVKLRWF
                     PVAGWEGFSTAVLPSLALSAAVLATVARYIRAEMIEVLSSDYILLARAKGNSTMRVLF
                     GHALRNALIPIITIIVPMLASILTGTLTIENIFGVPGLGDQFVRSITTNDFSVIMAIT
                     LLFSTLFIVSIFIVDILYGVIDPRIRVQGGKK"
     gene            986..2056
                     /locus_tag="NBI57_001553"
     CDS             986..2056
                     /locus_tag="NBI57_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCD1822493.1"
                     /translation="MAENKNNLSINDDHSNAAMTHTSDAIASSDFIIRELDLNQEPEM
                     QRESKNFWQDAWAQLKRNKLAVVGMIGLIIIVIFAFIGPVINKHDYAEQNVEHRNLPA
                     KIPVLDKVPFLPFDGKDADGKDAYKAANAKENYWFGTDQLGRDLWTRTWKGAQISLFI
                     GVVAAMLDIFIGVVYGAISGFFGGRVDTIMQRILEVIASIPNLIVVILFVLIFEPSIW
                     TIILAMSITGWLGMSRVVRGEFLKLKNQEFVMASKTLGASKFKLIFKHILPNTLGAIV
                     VTSMFTVPSAIFFEAFLSFIGIGVPAPQTSLGSLVNDGRAMLLIYPHELFIPAMILSL
                     LILFFYLFSDGLRDAFDPKMRK"
     gene            2072..3154
                     /locus_tag="NBI57_001554"
     CDS             2072..3154
                     /locus_tag="NBI57_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCD1822494.1"
                     /translation="MTERILEVNDLHVSFDITAGEVQAVRGVDFYLNKGETLAIVGES
                     GSGKSVTTKAITKLFQGDTGRIKKGEILFLGEDLAKKPENELIKLRGKDISMIFQDPM
                     TSLNPTMQIGKQVMEPLIKHKNYSKAQAKKRALEILNLVGLPNAEKRFKAYPHQFSGG
                     QRQRIVIATALACEPKVLIADEPTTALDVTMQAQILDLMKELQQKIDTAIIFITHDLG
                     VVANIADRVAVMYGGQMVETGDVNEIFYDPKHPYTWGLLSSMPDLSTTNDTPLLAIPG
                     APPDLLHPPKGDAFARRSQYALDIDFKVEPPWFKVSPTHFVKSWLLDARAPKVELPEL
                     VKQRMKPMPNNYEKPLKVERVSFNEK"
     gene            3144..4085
                     /locus_tag="NBI57_001555"
     CDS             3144..4085
                     /locus_tag="NBI57_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCD1822495.1"
                     /translation="MKNDEVLLSIKNLKQYFNAGKKNEVRAIENISFDIYKGETLGLV
                     GESGCGKSTTGKSIIKLNDITSGEILYEGIDIQKIRKRKDLLKFNKKIQMIFQDPYAS
                     LNPRLKVMDIVAEGIDIHHLATDKRDRKKRVYDLLETVGLSKEHANRYPHEFSGGQRQ
                     RIGIARALAVEPEFIIADEPISALDVSIQAQVVNLLLKLQRERGITFLFIAHDLSMVK
                     YISDRIAVMHFGKIVEIGPAEEIYQNPLHDYTKSLLSAIPQPDPESERSRKRFSYIDD
                     EANNHLRQLHEIRPNHFVFSTEEEAAQLRENKLVTQN"
     gene            4104..5759
                     /locus_tag="NBI57_001556"
     CDS             4104..5759
                     /locus_tag="NBI57_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HCD1822496.1"
                     /translation="MTRKFRTLILILIATIALSGCANDDGIYSDKGQVFRKILSSDLT
                     SLDTSLITDEISSEVTAQTFEGLYTLGKGDKPVLGVAKAFPEKSKDGKTLKVKLRSDA
                     KWSNGDKVTAQDFVYAWRKTVDPKTGSEFAYIMGDIKNASDISTGKKPVEQLGIKALN
                     DETLQIELEKPVPYINQLLALNTFAPQNEKVAKKYGKNYGTAADRAVYNGPFKVDDWK
                     QEDKTLLSKNQYYWDKKNVKLDKVNYKVIKDLQAGASLYDTESVDDAVITADQVNKYK
                     DNKGLNFVLTSGTFFVKMNEKQYPDFKNKNLRLAIAQAIDKKGYVDSVKNNGSIPSDT
                     LTAKGIAKAPNGKDYASTMNSPLKYNPKEARAHWDKAKKELGKNEVTFSMNTEDTPDA
                     KISAEYIKSQVEKNLPGVTLKIKQLPFKQRVSLELSNNFEASLSGWSADYPDPMAYLE
                     TMTTGSAQNNTDWGNKEYDQLLKVARTKLALQPNERYENLKKAEEMFLGDAPVAPIYQ
                     KGVAHLTNPQVKGLIYHKFGPNNSLKHVYIDKSIDKETGKKKK"
     gene            5971..7686
                     /locus_tag="NBI57_001557"
     CDS             5971..7686
                     /locus_tag="NBI57_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HCD1822497.1"
                     /translation="MGKLIKYISILLIVVLVLSACGKSSNKDEGVKDATKTETSKHKG
                     GTLNVALTAPPSGVYSSLLNSTHADSVVEGYFNESLLATDKKIRPKAYIASWKDIEPA
                     KKIEFKIKKGIKWHDGNELKIDDWIYSIEVLANKDYEGAYYPSVENIQGAKDYHEGKT
                     DHISGLKKIDDYTMQVTFDKKQENYLTGFITGHLLSKKYLSDVPIKDLAKSDKIRKYP
                     IGIGPYKVKKIVPGEAVQLVKFDDYWQGKPALDKINLKVIDQAQIIKAMEKGDIDVAN
                     DATGAMAKDAKSSNAGLKVLSAPSLDYGLIGFVSHDYDKKANKTGKVRPKYEDKELRK
                     AMLYAIDREKWIKAFFNGYASEINSFVPSMHWIAANPKDLNDYKYDPEKAKKILDKLG
                     YKDRDGDGFREDPKGNKFEINFKHYSGSNPTFEPRTAAIKDFWEKVGLKTNVKLVEFG
                     KYNEDLANASKDMEVYFRSWAGGTDPDPSDLYHTDRPQNEMRTVLPKSDQYLDDALDF
                     EKVGIDEKKRKDIYVKWQKYMNDELPGLPMFQGKSITIVNDKVRNLDIEIGTDQSLYN
                     LTKEA"
     gene            7737..8723
                     /locus_tag="NBI57_001558"
     CDS             7737..8723
                     /locus_tag="NBI57_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCD1822498.1"
                     /translation="MNNVLLEVKDLETSLKINNEWLATVENISFELSKGEVLGIVGES
                     GCGKSILSKSIIKLLPEKISKLSSGEVIFDGKRIDTLNEKQLLDIRGNDIAMIFQEPM
                     TALNPVFTIKNQLVESIKSHKKISKKEANNLAKDLLKKVGIARQDEILNSYPHQLSGG
                     MRQRVMIAMAISCSPKLLIADEPTTALDVTIQAQILDLLKELQKETQMAIMMITHDLS
                     VVAEFCDKVLVMYAGQIVEFGGIKEILHNPKHPYTQKLLSTIPKLKEGQKRLETIEGI
                     VPSIQAFHVNKCRFANRCNKKLDICNNQSPKMHVCEDVIVRCHLYKNEYKEI"
     gene            8726..9706
                     /locus_tag="NBI57_001559"
     CDS             8726..9706
                     /locus_tag="NBI57_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000427767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HCD1822499.1"
                     /translation="MENILEVNQIKKYYKIKTGLLQKTQYVKAVDDVSFSIKKGQTFG
                     LVGESGCGKSTLGKVIIRLEDATSGSIIVNGEDITRLQGKKLRKSRQQYQMIFQDPYA
                     SLNPMQMVGDIISEPILNYKKLPKEEIKKEVLYLLKCVGLSEDAYYKYAHEFSGGQRQ
                     RVGIARALALRPSLIVADEPVSALDVSVQSQVLNLLKDLQEQFKLSYLFIAHDLSVVK
                     HISDVIGVMYLGHIVEIASDKEIYENPKHPYTKALISSIPQIDKHNNNRIILKGELPS
                     PSNPPQGCPFHTRCPIAKDKCKENIPQLKDIGNEHQVACFYVDKVGDLNA"
     gene            9699..>10271
                     /locus_tag="NBI57_001560"
     CDS             9699..>10271
                     /locus_tag="NBI57_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCD1822500.1"
                     /translation="MLKLILKRLGLMIPLLILISIVVFSLAIIQPGDPFSDLQNGKIK
                     QEAINAQREKLGLNDSIPHQYIRWVNHVIHGDLGESIKYKRPVIDVIEERIPNTILLG
                     AMSLIITYIISFALGITSGRYSYSLTDYTVQIFNYLMLAIPSFIAGVFAIFIFSFELQ
                     WFPFQGSVDINLKEGTFEYYMSKIYHTFLPA"
BASE COUNT         3811 a         1408 c         1930 g         3122 t
ORIGIN      
        1 agaaaatttt gaagggaatg gaaatttaga aatcggaatt tgttagagga ggggattaga
       61 tggggaaata tattttcaaa cgatttattt atatgcttat ttctttattt attattatta
      121 caattacatt tttcttaatg aaattaatgc caggttcgcc atttaacgat gctaaattaa
      181 atgctgaaca aaaagaaatt ttaaatgaaa aatatggatt aaatgatcct gtagctacgc
      241 agtatttaca ttatttaaaa aatgttgtta caggcgattt tggtaattca ttccagtatc
      301 ataatcaacc tgtgtgggat ttgattaaac cgagactact accttctttt gaaatgggtc
      361 ttacagcaat gttcatcggt gtgatactgg gacttatttt aggtgttgca gcagctacta
      421 aacaaaattc ttgggttgac tatacaacta cagttatttc agttattgct gtatctgtac
      481 catcttttgt acttgctgta cttttacaat atgtatttgc agttaaatta agatggttcc
      541 cagtagctgg atgggaaggt ttttcgaccg cggtattacc gtcacttgca ttatctgcag
      601 ctgttttagc aactgtcgcc agatacataa gagcagagat gatagaggta ttaagttcag
      661 actatatttt attagcgaga gctaaaggta attcgacaat gcgtgtactt tttggacatg
      721 cacttagaaa tgctttaatt ccaattatta caattatcgt tcccatgtta gcaagtattt
      781 taacaggcac tttaacaatt gaaaatattt ttggggttcc tggattaggg gatcaattcg
      841 tacgttcaat tacaacaaat gatttctcag taatcatggc aatcacacta ttatttagca
      901 cactgtttat cgtttctatt tttattgtag atattttgta cggtgtgata gatccacgaa
      961 ttcgtgttca aggaggtaaa aaataatggc tgaaaataaa aacaatttgt cgattaacga
     1021 cgatcattct aatgcagcta tgacgcatac ctctgacgct atcgcatcat ctgattttat
     1081 tattagagaa ttagacttga atcaggaacc tgaaatgcaa cgagaaagca aaaacttttg
     1141 gcaagatgct tgggctcagt taaaacgaaa taagttagct gttgtcggta tgataggttt
     1201 aattatcatt gtaatatttg cttttatcgg tccagttata aataaacatg attatgctga
     1261 acaaaatgta gaacatagaa atcttccggc aaaaatacct gtattagaca aagttccatt
     1321 tttacctttt gatggtaaag atgcagatgg caaggatgct tataaagcag caaatgctaa
     1381 agaaaattat tggtttggta ctgatcagtt gggtcgagat ttatggacaa gaacatggaa
     1441 aggtgctcaa atttcattgt ttatcggtgt tgttgcagcg atgttagata tttttattgg
     1501 cgttgtatat ggtgcgattt ctggattctt cggtggacgt gtcgatacga ttatgcaacg
     1561 tatacttgaa gtcatagcat ctattccgaa tttaattgtc gtaattttat ttgtattaat
     1621 ttttgaacca tccatttgga caattatatt ggctatgtct atcacaggct ggttaggcat
     1681 gagcagagtt gtacgtggag aatttttaaa attgaaaaat caagagtttg tcatggcttc
     1741 gaaaacattg ggggcttcaa aattcaaatt gatatttaag catattttac ctaatacgtt
     1801 aggtgctatc gtggttacat caatgtttac agtacctagt gctattttct tcgaagcatt
     1861 tttaagtttc attggtatag gtgtacctgc acctcaaaca tcgttagggt cattagtaaa
     1921 tgatgggcgc gcaatgttat taatttatcc acatgaatta tttataccag caatgatttt
     1981 aagtttatta attctattct tttacttatt tagtgatgga ttacgtgatg catttgatcc
     2041 gaaaatgcgt aaataaaagg gggcatagca tatgactgaa agaatattag aagtaaatga
     2101 tttgcatgtt tcctttgata ttacagcagg ggaagtgcag gcagtgagag gtgtagattt
     2161 ttatttaaac aagggggaaa cattggcaat tgttggtgaa tcaggttcag gtaaatctgt
     2221 aacaacaaaa gcaattacaa aattattcca aggggacaca ggaagaatta aaaagggaga
     2281 aattttattt ttaggggaag atttagcaaa aaaacctgaa aatgagttaa ttaaattacg
     2341 tggcaaagat atttcaatga tctttcaaga tccaatgaca tctttaaacc caacgatgca
     2401 aattggtaaa caagtcatgg aaccattaat taagcacaaa aattatagta aagcacaagc
     2461 taaaaagcgc gcattggaaa tactaaatct tgtaggttta ccaaatgcag aaaaaagatt
     2521 taaagcatat cctcatcaat tttcaggtgg acaaaggcaa agaattgtta ttgcaaccgc
     2581 attagcttgt gaacctaaag tgctcattgc tgatgaacca acgactgcat tagacgtaac
     2641 gatgcaggca caaattttag atttaatgaa agaactacaa caaaaaatcg atacagcaat
     2701 tatttttata acgcatgatt taggggttgt tgcgaatatt gctgatagag tggcagttat
     2761 gtatggtggt caaatggttg aaacaggaga tgttaacgaa atattttatg atccaaagca
     2821 tccatataca tggggattat tatcgtcaat gcctgattta tcaacaacaa atgacacacc
     2881 attactagcg attcctggag cgccacctga tttattacac ccacctaaag gtgatgcatt
     2941 tgcgagacgt agtcaatatg cattagatat tgattttaaa gtagaaccac cgtggtttaa
     3001 agtttcaccg acacattttg tgaaatcttg gttattagac gcacgtgcac caaaagttga
     3061 actacccgag ctggtaaaac aacgtatgaa accgatgcct aataattatg aaaaaccact
     3121 caaggtagaa agggtgtcgt tcaatgaaaa atgatgaagt gctattatct attaaaaatt
     3181 taaagcaata ttttaacgca ggaaagaaaa acgaagtgag agcgattgaa aatatttcgt
     3241 ttgatatata caaaggggaa acattaggtt tagtaggaga atcggggtgt ggtaaatcta
     3301 caactggtaa atcaattatt aaacttaatg atattacaag tggagaaatt ttgtatgagg
     3361 gtattgatat acaaaagatt cgtaaacgta aagatttgct taaatttaat aaaaagatac
     3421 agatgatttt tcaagaccca tatgcgtctt taaatcctag gttaaaagta atggatatag
     3481 tagctgaagg tattgatatc catcatttag caactgataa gcgtgaccga aaaaaacgtg
     3541 tctatgattt acttgaaact gttggattaa gtaaagaaca tgccaatcgc tatcctcatg
     3601 aattttcagg tggacaacgc caacgtattg gaattgcccg tgcattagcc gttgaaccag
     3661 aattcattat cgcggacgaa ccaatatcgg cattggatgt ttcaatccaa gctcaagtag
     3721 ttaatttatt attaaaatta caacgtgaaa gagggattac gttcctattt atagctcatg
     3781 atctatcaat ggtgaagtat atttcagatc gtattgcagt catgcatttt gggaaaatag
     3841 ttgaaattgg accggcagaa gaaatttatc aaaatccatt acacgattat actaagtctt
     3901 tattatcagc cattccacaa cctgatcctg aatcagaacg cagtcgcaaa cgatttagtt
     3961 atattgatga tgaagcaaat aatcatttaa gacaattaca tgaaattaga ccgaatcact
     4021 ttgtctttag tactgaagaa gaagcggcac aactacgaga aaataaattg gtgacacaaa
     4081 attaagggga agggggaaat gcaatgacga gaaaatttag aacacttatt ttaattttga
     4141 ttgctacaat tgcattaagt ggttgtgcta atgacgatgg tatttattca gataaaggtc
     4201 aagtattcag aaaaattttg tcatcagact taacatccct tgatacatca ttaataacgg
     4261 atgaaatatc ttctgaagtg actgcgcaaa cattcgaagg tttatacaca ttaggaaaag
     4321 gtgacaaacc ggtgttaggt gttgcgaaag cttttcctga aaagagtaaa gatggtaaaa
     4381 ctttaaaggt taaattaaga agcgatgcta aatggagcaa tggtgacaaa gtgactgcac
     4441 aagactttgt ttatgcttgg agaaaaacag ttgaccctaa aacaggttct gaatttgcat
     4501 acattatggg ggacattaaa aatgcgagtg atattagtac tggtaagaaa cctgtagagc
     4561 aattaggtat caaagcatta aatgatgaaa cattacaaat tgaattagaa aagccggttc
     4621 catatattaa tcaattatta gcacttaata cctttgcacc tcaaaatgaa aaagttgcca
     4681 aaaaatatgg taaaaattac ggtacggcag ctgatagagc ggtatacaat ggtccattta
     4741 aagttgatga ttggaaacaa gaagataaaa ccttactatc taaaaatcag tattattggg
     4801 ataaaaagaa tgtaaaatta gataaagtga attataaagt tattaaagac ttacaagccg
     4861 gtgcatcatt gtatgatact gaatcagtag atgacgcagt tattactgca gatcaagtaa
     4921 ataaatataa agacaacaaa ggattaaact ttgtgttaac gtctgggaca ttttttgtaa
     4981 aaatgaatga aaaacaatat cctgatttta aaaacaaaaa tttaagattg gctatcgcac
     5041 aagcaataga taaaaaagga tacgttgatt cagtgaaaaa caatggctca attccttccg
     5101 atacactaac agccaaagga attgcgaaag cgcctaatgg caaagattat gcgagtacca
     5161 tgaattcgcc tttaaaatat aatcctaaag aagcaagagc acactgggac aaagctaaaa
     5221 aagagttagg taaaaatgaa gtgacatttt caatgaacac agaagataca ccagatgcaa
     5281 aaatatctgc tgaatatatc aaatcgcaag ttgagaaaaa tttaccagga gttactttga
     5341 aaattaagca attaccgttt aaacaaagag tatcactaga actgagtaac aattttgaag
     5401 catcacttag tggttggtct gcagattacc ctgatcctat ggcttattta gaaacaatga
     5461 ccacaggtag cgcacaaaat aatacagact ggggtaataa agaatatgat caattactta
     5521 aagtagcaag aaccaaattg gcacttcaac cgaacgaacg atatgaaaac ttgaaaaaag
     5581 cagaagaaat gttcctagga gatgcaccgg tagcaccaat ttatcaaaaa ggtgttgcac
     5641 atttaacaaa tcctcaagta aaaggattaa tttaccataa atttggtcca aataactcac
     5701 ttaaacatgt atatattgat aaatcgatag ataaagaaac aggtaagaag aaaaaataat
     5761 atgctttgta aattaggctg gagacatatc tccagtcttt ttgtgttgga taaaaacttt
     5821 gggaataaaa atttaaaata agtcgttttt taaattactg aaattgatta aatgcataaa
     5881 taactgaata ttctaaaaat aaacttgtaa taattttttc tatgagtaaa ctaaaaagaa
     5941 aaaattagat tgaaagtagg aggcatatgt atggggaagc taattaaata tatttcaata
     6001 cttcttattg tcgttttagt gttgagtgct tgcggaaaaa gcagtaataa agatgaagga
     6061 gtaaaagatg ctactaaaac ggaaacctca aaacataaag gtggtacctt aaatgtagca
     6121 ttaacagcac cgccaagtgg tgtttattct tcgttattaa atagtacaca tgcagattct
     6181 gtagttgagg gatattttaa cgaaagctta ttagcaactg ataaaaaaat acgtcctaag
     6241 gcatatattg cttcatggaa ggacatcgag ccggctaaga aaatagaatt taaaattaaa
     6301 aaaggtatta aatggcatga tggtaatgaa ttgaaaattg atgattggat ttattcaatt
     6361 gaagtcttag ctaacaagga ctacgaaggt gcttattatc caagtgtaga aaatatccaa
     6421 ggtgcgaaag attatcatga aggaaaaact gatcatatta gcggattgaa gaaaatagat
     6481 gactacacta tgcaggttac atttgataaa aaacaagaaa attacttaac aggatttatt
     6541 actggacatt tattaagtaa aaaatattta tcagatgtac caattaaaga tttagcgaaa
     6601 tcagataaaa tccgaaaata tcctattggt attggaccgt ataaagttaa gaaaatcgtt
     6661 ccaggtgagg ctgttcaact cgttaaattt gatgattatt ggcaaggtaa gcctgcacta
     6721 gacaaaatca atttaaaagt tattgatcaa gctcaaatta ttaaggcaat ggaaaaaggc
     6781 gatattgatg ttgcgaatga tgctaccggt gcaatggcaa aagatgctaa gtcatctaat
     6841 gctggtctca aggtattatc tgcgccaagc ttagactacg gtttaatagg attcgtatct
     6901 catgattacg ataaaaaagc taataaaact ggtaaagtga gaccaaaata tgaagacaaa
     6961 gaattacgta aagcaatgct ttatgcaatt gatagagaaa aatggattaa agcgtttttc
     7021 aatggttacg ctagtgaaat caatagtttt gtaccatcta tgcattggat agcagccaat
     7081 cctaaggacc taaatgatta caaatatgat cctgaaaaag ctaaaaaaat cttagataag
     7141 ttaggttata aagatagaga tggtgacgga tttagagaag atcctaaagg taataaattt
     7201 gagattaact ttaaacatta ttcaggttca aatcctactt ttgaaccaag aactgctgcg
     7261 ataaaagatt tctgggaaaa agttggcttg aaaacaaatg tgaagttagt agaattcggt
     7321 aaatataatg aagacttagc aaatgcatct aaagatatgg aagtgtactt cagatcatgg
     7381 gcaggaggta cagatccaga tccatcagat ttataccaca ctgatagacc tcaaaatgaa
     7441 atgagaacag ttttaccaaa atcagatcaa tatttagatg atgcattaga cttcgaaaaa
     7501 gtaggcattg atgaaaagaa acgtaaagat atttatgtta aatggcaaaa atatatgaat
     7561 gatgagttac ctggattacc aatgttccaa ggtaaatcga taactattgt taacgataaa
     7621 gtacgaaact tagacattga aattggaact gatcaaagtt tatataattt aactaaagaa
     7681 gcttagtaat gtaacctcga cgattttgtc gaggtttact tatatggggg gatgttatga
     7741 ataatgtatt gttagaggtt aaagatttag aaacatcatt aaaaataaat aatgaatggt
     7801 tagcaactgt tgaaaatatt tcttttgaat tatctaaagg agaagttttg ggtatagtag
     7861 gggaatctgg ttgcggtaag tccatattaa gtaagtcaat tattaaatta ttaccagaaa
     7921 agatatctaa actaagtagt ggagaagtta tatttgatgg taaacgaatc gatacgctca
     7981 atgagaagca attgctagat attcgaggaa atgatattgc tatgattttt caagaaccta
     8041 tgactgcttt aaatcctgta tttaccataa aaaatcaact tgtggaatct ataaaatcac
     8101 ataaaaaaat ttctaaaaaa gaagcaaata acttagcaaa agatttacta aaaaaagttg
     8161 gaattgctag acaagatgaa atattaaata gctatcctca tcaattatct ggtggtatga
     8221 gacaaagagt aatgattgca atggccattt catgttctcc taaattatta attgctgatg
     8281 aacctacaac agcattggat gtcacgattc aagcgcaaat attagactta ttaaaagaat
     8341 tgcaaaagga aacgcaaatg gcaattatga tgattacaca tgatttgagt gtagttgctg
     8401 agttttgcga taaagtctta gttatgtatg caggtcaaat tgtagaattt ggaggcataa
     8461 aagaaatact acacaatccg aaacatcctt atacccaaaa attattatca acaattccaa
     8521 aacttaaaga agggcagaaa cgacttgaaa cgatagaagg aattgtgcca tcaatccaag
     8581 catttcacgt taataagtgc agatttgcaa atagatgtaa caaaaaactg gatatttgta
     8641 ataatcaatc tcctaaaatg catgtttgtg aagacgtcat tgtacgttgt catttgtaca
     8701 aaaatgaata taaggagata taagaatgga aaatatttta gaagtcaacc aaataaaaaa
     8761 atactacaaa attaaaactg gattattaca aaaaactcag tacgttaaag ctgttgatga
     8821 cgtatcgttt tcaataaaaa aaggacaaac ttttggatta gtaggagaat cgggttgtgg
     8881 taagtcaacg ttaggtaaag tgattatcag gcttgaagat gcaacttcag gttcaataat
     8941 tgttaatggt gaagatataa caagattaca aggtaaaaaa ctcagaaaat cacgacaaca
     9001 atatcagatg atatttcaag atccgtatgc atcattgaat ccgatgcaaa tggttggaga
     9061 tatcatttca gaacctattt taaattataa aaaattgcca aaagaagaaa taaaaaaaga
     9121 agtactatat ttattaaaat gtgttggcct aagtgaagat gcatattata aatatgcaca
     9181 tgaattttca ggtggacaga gacaaagagt gggaattgca agagcattgg ctttgcgtcc
     9241 gagtttaatt gttgctgatg agcctgtaag tgcattagat gtatctgttc aatctcaagt
     9301 actgaattta ttaaaagatt tacaagagca atttaagtta agctatttat ttatcgcgca
     9361 tgatttaagt gtagtgaaac atattagtga tgtaattgga gttatgtatt taggtcatat
     9421 agttgaaatt gcatctgata aagaaattta tgaaaatccc aaacatccat atacaaaagc
     9481 attaatttca tcaataccac aaattgataa acataataac aatagaatta tattaaaagg
     9541 agaattacct tcgccaagta atccgccgca aggttgtcct tttcatacaa gatgtccgat
     9601 tgcaaaagat aaatgtaaag aaaatatacc acaattaaag gacattggta acgaacatca
     9661 agttgcttgt ttctacgtag ataaagtagg tgatttaaat gcttaaatta atacttaaaa
     9721 ggttagggtt aatgatcccg ttactaattt taatctcaat agttgtattt tcattagcaa
     9781 tcatacaacc aggagatcca ttttcagatt tacaaaacgg taaaataaaa caagaagcaa
     9841 taaatgcaca aagggaaaag ttaggcctca acgactcaat accacatcaa tatattagat
     9901 gggtcaatca tgttatacat ggtgatttag gggagtcaat caaatataag aggccggtaa
     9961 ttgatgttat tgaggaaaga attccaaata caatattact cggtgctatg tcattaatta
    10021 ttacttatat tatctcattt gctttaggaa taacgtcagg tagatattct tacagtttga
    10081 ctgattatac tgtgcaaata tttaattatt tgatgttagc tattccatct tttattgcgg
    10141 gagtatttgc aatttttatt ttttcttttg aattacaatg gtttccgttt caaggttctg
    10201 ttgatattaa ccttaaagaa ggtacttttg aatattatat gagtaaaatt tatcatacat
    10261 ttttgcctgc a
//
LOCUS       DAHHSC010000085        10034 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.086, whole genome shotgun sequence.
ACCESSION   DAHHSC010000085 DAHHSC010000000
VERSION     DAHHSC010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 10034)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10034)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10034
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.086"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            complement(<1..729)
                     /gene="csbB"
                     /locus_tag="NBI57_001561"
     CDS             complement(<1..729)
                     /gene="csbB"
                     /locus_tag="NBI57_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoteichoic acid-specific glycosylation protein
                     CsbB"
                     /protein_id="HCD1822501.1"
                     /translation="MKIRVVIPCFNEGEVITQTHQQLTEILSQDSSVKGYDYNMLFID
                     DGSTDTTIDEMQHLATIDRHVSFISFSRNFGKEAAMIAGYQHSTEFDAVIMIDCDLQH
                     PPEYIPKMVEGFMEGYDQVIAKRDRSGENFSRKTLSHLYYKLVNCFVEEVQFDDGVGD
                     FRLLSQRAVKSIASLEEYNRFSKGLFEWIGYNTKVFTYQNVERQKGESKWSFKKLFNY
                     GIDGLISFNSKPLRMMIYLGLFIFS"
     gene            complement(861..1700)
                     /locus_tag="NBI57_001562"
     CDS             complement(861..1700)
                     /locus_tag="NBI57_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HCD1822502.1"
                     /translation="MLNEIQILNNGYPMPSVGLGVYKISDEDMTKVVNAAIDAGYRAF
                     DTAYFYDNEASLGRALKDNGVDREDLFITTKLWNDYQGYEKTFEYFNKSIENLQTDYL
                     DLFLIHWPCEADGLFLETYKAMEELYEQGKVKAIGVCNFNVHHLEKLMAQSSIKPMVN
                     QIEVHPYFNQQELQEFCDRHDIKVTAWMPLMRNRGLLDNPVIVKIAEKYHKTPAQVVL
                     RWHLAHNRIIIPKSQTPKRIQENIDILDFNLELTEVAEIDALDRNARQGKNPDDVKIG
                     DLK"
     gene            complement(1922..3271)
                     /locus_tag="NBI57_001563"
     CDS             complement(1922..3271)
                     /locus_tag="NBI57_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HCD1822503.1"
                     /translation="METSTIISLIIFILLIALTTVFVGSEFALVKIRATRIEQLADEG
                     NKPAKIVKKMIANLDYYLSACQLGITVTSLGLGWLGEPTFEKLLHPIFEAINLPTALT
                     TTISFAVSFIIVTYLHVVLGELAPKSIAIQHTEKLALVYARPLFYFGNIMKPLIWLMN
                     GSARVIIRMFGVNPDAQTDAMSEEEIKIIINNSYNGGEINQTELAYMQNIFSFDERHA
                     KDIMVPRTQMITLNEPFNVDELLETIKEHQFTRYPITDDGDKDHIKGFINVKEFLTEY
                     ASGKTIKIANYIHELPMISETTRISDALIRMQREHVHMSLIIDEYGGTAGILTMEDIL
                     EEIVGEIRDEFDDDEVNDIVKIDNKTFQVNGRVLLDDLTEEFGIEFDDSEDIDTIGGW
                     LQSRNTNLQKDDYVDTTYDRWVVSEIDNHQIIWVILNYEFNEARPTIGQSDEDEKSE"
     gene            complement(3492..4673)
                     /gene="nagA"
                     /locus_tag="NBI57_001564"
     CDS             complement(3492..4673)
                     /gene="nagA"
                     /locus_tag="NBI57_001564"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HCD1822504.1"
                     /translation="MSELIIYNGKVYTEDGKIDNGYIHVKDGQIVAIGEGDDKAAIDN
                     DTTNKIQVIDAKGHHVLPGFIDIHIHGGYGQDAMDGSYDGLKYLSENLLSEGTTSYLA
                     TTMTQSTDKIDKALTNIAKYEAEQDVHNAAEIVGIHLEGPFISENKVGAQHPQYVVRP
                     FIDKIKHFQETANRLIKIMTFAPEVEGAKEALETYKDDIIFSIGHTVATYEEAVEAVE
                     RGAKHVTHLYNAATPFQHREPGVFGAAWLNDALHTEMIVDGTHSHPASVAIAYRMKGN
                     ERFYLITDAMRAKGMPEGEYDLGGQKVTVQSQQARLANGALAGSILKMNHGLRNLISF
                     TGDTLDHLWRVTSLNQAIALGIDDRKGSIKVNKDADLVILDDDMNVKSTIKQGKVHTF
                     S"
     gene            complement(4981..6939)
                     /locus_tag="NBI57_001565"
     CDS             complement(4981..6939)
                     /locus_tag="NBI57_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HCD1822505.1"
                     /translation="MRVTELLTKDTIAMDLMANDKNGVIDELVNQLDKAGKLSDVASF
                     KEAIHNRESQSTTGIGEGIAIPHAKVAAVKSPAIAFGKSKEGVDYQSLDMQPAHLFFM
                     IAAPEGGAQTHLDALAKLSGILMDENVREKLLHASSPEEVLAIIDEADDEVTKEEEAE
                     AEAQQVATAEQSSKQSNEPYVLAVTACPTGIAHTYMARDALKKQADKMGIKIKVETNG
                     SSGIKNHLTEQDIENATGIIVAADVHVETDRFDGKNVVEVPVADGIKRPEELINKALD
                     TSRKPFVARDGQRKGNSNDSQEKLSPGKAFYKHLMNGVSNMLPLVISGGILMAIVFLF
                     GANSFNPKSSEYNAFAEQLWNIGSKSAFALIIPILSGFIARSIADKPGFASGLVGGML
                     AISGGSGFIGGIIAGFLAGYLTQGVKAMTRKLPQALEGLKPTLIYPLLTVTATGLLMI
                     YVFNPPASWLNHLLLDGLNNLSGSNIVLLGLVIGAMMAIDMGGPFNKAAYVFATGALI
                     EGNAAPITAAMIGGMIPPLAIATAMLIFRRKFTKEQRGSIIPNYVMGMSFITEGAIPF
                     AAADPLRVIPSMMIGSGIGGAIALGLGSRITAPHGGIIVIVGTDGAHLLQTLIALLVG
                     TLVSALIYGLIKPKLTETEIEASKSMDE"
     gene            complement(6945..7865)
                     /gene="pfkB"
                     /locus_tag="NBI57_001566"
     CDS             complement(6945..7865)
                     /gene="pfkB"
                     /locus_tag="NBI57_001566"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HCD1822506.1"
                     /translation="MIYTVTFNPSIDYVIFTNDFKIDGLNRATATYKFAGGKGINVSR
                     VLKTLDVESTALGFAGGFPGKFIIDTLNNSAIQSNFIEVDEDTRINVKLKTGQETEIN
                     APGPHITSTQFEQLLQQIKNTTSEDIVIVAGSVPSSIPSDAYAQIAQITAQTGAKLVV
                     DAEKELAESVLPFHPLFIKPNKDELEVMFNTTVNSDTDVIKYGRLLVDKGAQSVIVSL
                     GGDGAIYIDKEISIKAVNPQGKVVNTVGSGDSTVAGMVAGIASGLTIEKAFQQAVACG
                     TATAFDEDLATRDAIEKIKSQVTISVLDGE"
     gene            complement(7862..8626)
                     /locus_tag="NBI57_001567"
     CDS             complement(7862..8626)
                     /locus_tag="NBI57_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCD1822507.1"
                     /translation="MMIITEKRHELILEELSHKDFLTLQELIDRTGCSASTIRRDLSK
                     LQQLGKLQRVHGGAMLKENRMIEANLTEKLATNLDEKKMIAKIAANQINDNECLFIDA
                     GSSTLELIKYIQAKDIIVVTNGLTHVEALLKKGIKTIMLGGQVKENTLATIGSSAMEI
                     LRRYCFDKAFIGMNGLDIELGLTTPDEQEALVKQTAMSLANQSFVLIDHSKFNKVYFA
                     RVPLLESTTIITSEKALNQESLKEYQQKYHFIGGTL"
     gene            complement(8876..9358)
                     /gene="ybaK"
                     /locus_tag="NBI57_001568"
     CDS             complement(8876..9358)
                     /gene="ybaK"
                     /locus_tag="NBI57_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HCD1822508.1"
                     /translation="MAKNKKTNAMRMLDRAKIKYEVHSFEVPEEHLSGAEVAELIQAN
                     VKTVFKTLVLENTKHEHFVFVIPVSETLDMKKAAALVGEKKLQLMPLDNLKNVTGYIR
                     GGCSPVGMKTLFPTVVDKSCENYSHISVSGGLRTMQITIAVEDLITITKGKIGAVIHE
                     "
     gene            complement(9532..9984)
                     /locus_tag="NBI57_001569"
     CDS             complement(9532..9984)
                     /locus_tag="NBI57_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1822509.1"
                     /translation="MTVKNLFLGFVAVILTVCLIGLLILATNEDALAKVHKTINTLNA
                     INVSTEDTYKKKMDILNIHTAKASEVNENVEKQNHFKHRVNANKSNSFNEQECQVIAD
                     RYADKHINDNYGLERISKTNHGYNYVYSNDNSTSKQHVSISNQGIITK"
BASE COUNT         3097 a         1985 c         1352 g         3600 t
ORIGIN      
        1 tgaaaagata aacaagccaa gataaatcat cattctcaaa ggtttactat taaaggaaat
       61 caatccatca ataccataat taaataactt tttaaaggac cacttagatt cccctttttg
      121 tctctcaaca ttttgatacg taaacacttt agtattatag cctatccatt caaataaccc
      181 ttttgaaaat cgattatatt cttcaagtga tgcaatggat ttaacagctc tttggcttaa
      241 aagtctaaaa tcaccaacac catcatcaaa ttgtacttct tctacaaagc aattaactaa
      301 cttataatac aaatggctta atgttttgcg actaaaattt tcaccacttc tatcacgctt
      361 tgcaatcact tgatcatagc cttccataaa accttcaacc attttcggaa tatattcagg
      421 tggatgttgc aaatcacaat ctatcatgat gactgcatca aattcagtac tatgctggta
      481 acctgcaatc atagctgctt cttttccaaa atttctacta aaagaaataa agctgacatg
      541 cctatctatt gtggcaagat gttgcatttc atctatagtg gtatccgtac ttccatcatc
      601 tatgaaaagc atattataat catagccttt cacactacta tcttgtgaaa gtatttcagt
      661 taattgttga tgtgtttgtg taatgacttc cccttcatta aaacaaggaa tgacgactct
      721 aattttcatc tatgtccatc ctttttattt aaaagatttt gaatgtacct gagtattgtt
      781 ttatcaaata gtaaaaacca actcatttat tttaatgtac acattaaaag gcattcataa
      841 acgtaaaatt taacatccag ttattttaaa tccccaattt tcacatcatc tggattttta
      901 ccttgtcttg catttctatc taaagcatca atttcagcta cttctgttaa ttctaaatta
      961 aaatctaaaa tatctatatt ttcttgaatg cgtttaggtg tctgagattt tggaataata
     1021 attctattgt gtgctaaatg ccaacgtaat acaacttgtg ctggtgtttt atgatatttt
     1081 tcagcaattt taacaatgac agggttgtct agtagtcctc tatttctcat caaaggcatc
     1141 catgcagtca ctttaatatc gtgacgatca caaaattctt gtaattcttg ttggttaaaa
     1201 tatggatgta cctcaatttg attcaccatt ggtttgatac ttgattgagc cattaatttt
     1261 tctagatgat gaacattaaa attacataca cctattgctt ttaccttacc ttgctcgtaa
     1321 agttcttcca tagctttata tgtttctaaa aatagaccat ctgcttcaca aggccaatgt
     1381 attagaaata aatcaagata atcagtttgt aaattttcaa tcgatttgtt gaaatattcg
     1441 aatgttttct cataaccttg atagtcattc cataacttcg ttgttataaa caaatcttct
     1501 ctatcgacgc cattatcctt taatgctcgt cctagtgaag cctcattatc ataaaagtat
     1561 gctgtatcaa acgctctata gcctgcgtca attgcagcat ttacaacttt agtcatatct
     1621 tcgtcagaga ttttataaac acctaaccca actgaaggca tcgggtatcc attatttaat
     1681 atttgtatct cattcaacat ctttatatct ctccaatcta tgtatctttt atatctttac
     1741 attaccctaa atttttcaac aaactcaatt aatgcgaatt atcgctttca ataaaaaata
     1801 ttcattaaat cattaaagat attgagttcc aatactattt tcacttttca ttaattttag
     1861 tcaaaaaaat aaccaaccaa aaatgaatta aatcattctt agttggttta tatattaata
     1921 tctattctga tttttcatct tcatcagact gtccgatagt aggtctcgct tcattaaatt
     1981 catagtttaa tatcacccaa ataatttggt ggttatcgat ttctgaaaca acccagcgat
     2041 cataagttgt atccacgtaa tcatcttttt gtaaattggt attacgagat tgtaaccatc
     2101 cacctatcgt atcaatatcc tcagagtcat caaattctat accgaactct tcagttaaat
     2161 catccaatag tactctgcca tttacttgga atgtcttatt atcaatttta acgatatcat
     2221 tcacttcatc atcatcaaat tcatcacgaa tttctccaac gatttcttct aaaatatctt
     2281 ccatcgttaa aatacctgcc gttccaccat attcatctat aataagactc atatgtacat
     2341 gttcacgttg cattctaatt aatgcatcac tgatacgtgt tgtctctgaa atcattggca
     2401 actcatgtat atagtttgct attttaatcg tttttccaga agcgtattca gttaaaaatt
     2461 ctttgacgtt aataaatcct ttaatgtggt ctttatcacc atcatcagta attggataac
     2521 gcgtaaattg atgttctttt attgtttcta gtaattcgtc tacattaaaa ggttcattta
     2581 gtgtaatcat ttgagttcta ggtaccatta tatcttttgc atgtctttca tcgaatgaaa
     2641 agatattttg catatatgcc aattcagttt ggttgatttc tccaccatta taactattgt
     2701 taataataat tttgatttct tcttctgaca ttgcatcagt ttgggcatca ggatttacac
     2761 caaacattct aataataaca cgtgcagaac cattcatcag ccaaatcaat ggtttcataa
     2821 tgttaccgaa atagaacaat ggtcttgcat atactaaagc aagcttttca gtatgttgaa
     2881 tcgctataga tttaggcgct aattcaccaa gtactacatg caaatacgta acgattataa
     2941 atgacactgc aaacgaaatc gtcgtcgtta atgcagttgg taaattgatt gcttcaaata
     3001 tcgggtgtaa tagcttttca aacgttggtt caccaagcca acctaaccct aaagatgtta
     3061 ctgttatacc taactgacaa gcagaaagat aataatctag attagcaatc atctttttta
     3121 ctattttagc aggtttattt ccttcatctg ctagctgttc aattcttgtt gctctaattt
     3181 ttactaatgc aaattctgaa ccaacaaata cagtggttaa tgcaattaat agaataaata
     3241 taatcaaact aattatggtc gaagtttcca attaattccc tatttctagg gattcacctc
     3301 catgttatat gtgtcactca tgggtaacac gcattcaaat ttatcactat gacttaaatt
     3361 acaactacat gttatcgcct tcccatttag acacccccag aaaaaaatgt tattgcttct
     3421 attttatcat aatataaagt gcttatgtta acagattaaa tctattgcat acatttaatt
     3481 atgattattt attagctaaa tgtgtgaacc ttgccttgtt ttattgtaga ttttacattc
     3541 atatcatcat ctagaataac aagatctgca tccttattta ctttaatact accttttcta
     3601 tcatcgatac ctaatgcaat ggcttgattt aaacttgtta ctcgccataa atgatctaat
     3661 gtatcacctg taaatgatat taagttacgt aacccatgat tcatttttaa aatactacca
     3721 gcaagcgcac catttgcaag acgtgcttgt tgcgattgaa cagttacttt ttgtccaccc
     3781 aaatcatatt ctccttcagg catacctttt gcacgcattg catcggtaat taaataaaaa
     3841 cgttcattac ctttcatacg gtaagcaatt gcaaccgatg ccggatgaga atgtgtgcca
     3901 tcaacaatca tttcggtatg tagagcatca ttcaaccatg ctgctccaaa aacacctggt
     3961 tctctatgtt ggaatggcgt cgctgcatta tataaatgcg tgacatgttt agctcctcgc
     4021 tcaacagctt cgactgcttc ttcgtatgtt gccactgtat gaccaattga aaaaataatg
     4081 tcatctttat acgtttcaag cgcttctttt gcaccttcaa cttcaggtgc aaacgtcata
     4141 atctttatta atctgttagc agtctcttga aaatgtttaa ttttatcgat aaatgggcgt
     4201 acaacgtatt gcggatgttg agcaccaact ttattttcag atataaatgg tccttctaaa
     4261 tgtataccta caatttccgc tgcattgtga acatcttgct ccgcttcata tttagcaata
     4321 tttgtaagtg ctttatctat tttatcagta gattgcgtca ttgtagtggc caagtatgat
     4381 gtcgtccctt cagataacaa attttcggat agatatttta agccatcgta tgacccatcc
     4441 attgcatctt gaccataacc accatgaata tgtatatcaa taaaacctgg taatacatga
     4501 tgacctttag catcaatcac ttgaatttta tttgtcgtat cattatcaat tgctgcttta
     4561 tcatcccctt ctccaattgc aacaatctgt ccatctttca catgaatgta accattatcg
     4621 attttgccat cttcagtata aactttgccg ttatatataa ttaattctga cacgtataaa
     4681 cccttcttta tttataaatt ttcactttaa gacactcata ttttactata tctgcttcgt
     4741 ttggcataac tttttgcaaa atattaaaaa agtctataca tttgtggtgt ctatttcttc
     4801 attcatttat ctaaaaataa aaacaccttc atatcaattc ataaaaactg aattaataga
     4861 aagtgttttt acagctttgt ataagctacc gttaacacga tgtataaaac taacatatat
     4921 tcattgaact tacaacttaa tatgaagctc tttgctaaca atcattttac atcattaaaa
     4981 ctactcgtcc attgattttg aagcttcgat ttctgtttca gttaactttg gtttaataag
     5041 tccataaatt aatgctgaaa ctaatgtacc aactagaagt gcaataagag tttgaagtaa
     5101 gtgtgcacca tcagtaccaa caattacaat aataccacca tgtggcgcag taattcgaga
     5161 acctaagcct aaagcaattg cgccacctat acctgaacca atcatcattg aaggaataac
     5221 acgtaatgga tcggcagctg caaatggaat cgcaccttct gtaataaagg acatacccat
     5281 cacatagtta gggataattg aaccacgttg ttcttttgta aatttacgtc tgaaaattaa
     5341 catcgctgtc gcaattgcta acggtggaat cataccacca atcattgcag ctgtaattgg
     5401 tgctgcatta ccttcaatca acgcacctgt tgcaaaaaca tatgccgctt tgttgaatgg
     5461 accgcccata tcaatcgcca tcatagcgcc aataactaaa cctaataata caatattaga
     5521 acctgataaa ttgtttaatc catctaataa caaatgattt aaccaagatg ctggtggatt
     5581 aaagacataa atcatcaata agcctgtagc tgtcactgtt aatagtggat aaattaatgt
     5641 tggctttaat ccctctaatg cttgtggtaa cttacgtgtc atggctttaa caccttgtgt
     5701 taagtaacct gctaagaaac ctgcaataat accaccaata aatcctgaac cacctgaaat
     5761 tgctaacata ccacctacaa gacctgaagc gaaaccaggt ttatccgcaa tactacgtgc
     5821 aatgaatcca gataaaattg gaatgattag cgcgaatgca cttttactac caatgttcca
     5881 aagctgctct gcaaacgcat tgtactctga gctttttgga ttaaatgaat ttgctccaaa
     5941 taaaaataca attgccatta aaataccacc agatattaca agtggcaaca tgttagaaac
     6001 accgttcatt aagtgtttat agaatgcttt acctgggctt aatttttctt gactgtcatt
     6061 tgagttacct tttctttgac catcacgggc aacaaaaggt ttacgacttg tatctaatgc
     6121 tttattaatt aattcttctg ggcgtttaat accatctgct actggtactt ctacgacatt
     6181 tttaccatcg aagcgatccg tctcaacatg aacatcagca gcaacaatga tacctgttgc
     6241 attttcaata tcttgttcag ttaaatggtt tttaatgccg cttgaaccgt tcgtttctac
     6301 tttaatttta atacccatct tatccgcttg ttttttcaat gcgtcacgtg ccatgtacgt
     6361 atgtgcaata cctgttggac aagcagttac tgctaacaca tatggctcgt tagattgttt
     6421 agatgattgt tctgcagttg caacttgttg tgcttcagct tctgcctctt cttcttttgt
     6481 cacttcatca tcagcctcat ctataatcgc tagtacttct tcaggtgatg aagcatgtaa
     6541 taatttctcg cgtacatttt catccattaa aataccagac aacttagcta aagcatctag
     6601 atgtgtttgg gcgccacctt ctggcgctgc aatcataaag aataaatgag ctggttgcat
     6661 atctaaactt tggtaatcta caccttcttt agatttaccg aatgcgatag ctggagactt
     6721 aactgcggct actttcgcat gtggaatagc aataccttcg ccgataccag ttgtactttg
     6781 tgattctcga ttgtgaatcg cttccttaaa tgacgcgaca tcacttaatt tacctgcttt
     6841 gtctaattga tttactaact catcaataac accatttttg tcatttgcca ttaaatccat
     6901 tgctatcgta tcttttgtta ataactctgt tactctcatt attttcactc cccatcaagt
     6961 acgctaatcg taacttgtga ttttattttt tctatagcgt cccgtgttgc taagtcctca
     7021 tcaaatgccg tggcagtacc gcatgcgact gcttgttgga atgctttttc aatcgttaaa
     7081 cctgaagcaa ttccagccac catgcctgca actgtactat caccagagcc aactgtatta
     7141 accactttcc cttgtggatt aactgcttta atactgattt ctttatcaat ataaatagca
     7201 ccatcaccgc caagcgagac aataacagat tgcgcacctt tatcaactaa caaacgacca
     7261 tatttaataa catctgtgtc tgagttcact gttgtattaa acatcacttc taattcatct
     7321 ttattaggtt taataaatag tggatgaaat ggtaaaacgc tttcagccaa ttctttttca
     7381 gcgtcgacta ctaatttagc acctgtctgt gctgtaattt gtgcaatttg cgcatacgca
     7441 tcgcttggaa tactacttgg tacacttcca gcaacaataa ctatatcttc gcttgttgta
     7501 tttttaattt gttgtaacag ttgttcaaat tgtgttgacg ttatatgagg acccggtgca
     7561 ttgatttctg tttcttgtcc tgtttttaat ttcacattaa tacgtgtatc ttcatcaact
     7621 tcaataaaat tcgattgaat tgcactgtta tttaatgtat ctataatgaa tttcccagga
     7681 aatccacctg caaatcccaa ggcagttgac tcaacatcta atgtctttaa gacgcgcgag
     7741 acattaatac ctttcccccc agcgaattta tatgttgctg ttgctctgtt caaaccatca
     7801 attttaaaat cattcgtaaa aatgacatag tcaattgaag gattgaaagt cactgtataa
     7861 atcataaagt ccctcctata aagtgatact tttgttggta ttcttttaac gattcttgat
     7921 ttaatgcttt ttcagatgtg atgattgtcg tactttctag caaaggtaca cgagcaaaat
     7981 atactttatt aaacttagaa tgatctataa gtacaaatga ttgattggct aatgacattg
     8041 ctgtttgttt aactaatgcc tcttgctcat cgggagtagt taatccaagt tcaatatcta
     8101 atccattcat cccgataaaa gctttatcga aacaatatcg tcttaatatc tccatagcac
     8161 tagaaccaat cgtagcaagt gtattttctt taacttgacc acctagcata attgttttaa
     8221 tacctttttt aagtaaagct tctacatgtg ttaaaccatt ggttaccaca atgatatctt
     8281 tcgcttgaat atatttaatt agctccaatg tagatgaacc agcatcgata aataagcatt
     8341 cattatcgtt gatttgatta gctgctattt tagcaatcat tttcttttca tcaagattcg
     8401 ttgctaattt ttcagttaaa ttcgcctcaa tcatacgatt ttcttttaac attgcaccac
     8461 catgcacacg ttgcaatttc cctaattgtt gtagtttaga taaatctctt cgtattgttg
     8521 aagcactgca accagttcga tctattaatt cttgtaaagt caaaaaatct ttgtgcgaaa
     8581 gttcttctaa tattaactcg tgtctttttt cagtaattat catcatcacc ccatctcact
     8641 ttgtattata atgagtacgc tttcactttt caatcataaa ttaccaaaaa caatcacaaa
     8701 cacccaaatt gtgacgataa aattacattt ttatgttcaa atactcaatt ataatgactg
     8761 tttatgatcg taaaatgcta attcaatcaa aaaggagtgt ggcagaaatg aattttataa
     8821 aaatttcatc tctgccacac tcctaatctt gccccatact ttttgttgtt attaatcatt
     8881 catggataac tgctccaatt ttgcctttag ttattgtaat caaatcctca acagctattg
     8941 tgatttgcat tgttcgaagc ccaccactca cactgatatg actataattt tcacacgatt
     9001 tgtcaacgac tgttggaaac aatgttttca taccaacagg cgaacaccca ccacgaatgt
     9061 atcccgttac atttttcaaa ttatctaaag gcataagctg caatttcttc tctccaacca
     9121 aagcagctgc ctttttcata tctaaagttt cacttactgg gataacaaat acaaaatgtt
     9181 catgttttgt attttctaga acaagcgttt taaatactgt tttaacattt gcttgtataa
     9241 gttctgcgac ttctgcaccc gataaatgtt cttctggtac ctcaaagcta tgaacttcgt
     9301 atttaatttt tgcacgatca agcatacgca tcgcgttcgt tttcttattt ttagccattt
     9361 caacacctca tttatacttc ttcagaacta tattaacatt ttaaaataca ttacggattg
     9421 gttgattttg acataacatt tacatcattt tataagttga tttcaccttt ttaaattaaa
     9481 ttattgcaaa ataaaaacag tatatccaat tagaatacac tgttccatct attatttcgt
     9541 tattatgcct tgatttgaaa tacttacatg ttgcttacta gttgaattat cattggaata
     9601 aacataatta tatccatgat ttgtcttaga aattctttct aaaccataat tatcattgat
     9661 atgcttatct gcataacgat cagcaataac ttggcactct tgttcgttaa aagaatttga
     9721 tttatttgca ttcacacgat gtttaaaatg attttgcttt tccacatttt cattcacttc
     9781 agatgcttta gcagtatgaa tattgagaat atccattttc tttttataag tatcttcagt
     9841 tgatacattt atcgcgttaa gcgtattaat tgttttatgt accttagcaa gcgcatcttc
     9901 atttgttgct aatattaata aaccaattaa acaaacggtt aatattacag caacaaagcc
     9961 taaaaataaa ttttttactg tcatcccctt acccacattt ccttactcat tttttatcat
    10021 cttaacacat taac
//
LOCUS       DAHHSC010000086         9992 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.087, whole genome shotgun sequence.
ACCESSION   DAHHSC010000086 DAHHSC010000000
VERSION     DAHHSC010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 9992)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9992)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9992
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.087"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            <1..243
                     /locus_tag="NBI57_001570"
     CDS             <1..243
                     /locus_tag="NBI57_001570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1822510.1"
                     /translation="SWGEGPNTEADEKSAYNNVQVGDGPQQREIGFPISTDNASWRGP
                     NIEAGGKSVYNNVQVGVGRRNKFCENIISVPLPSKE"
     gene            533..3238
                     /gene="acnA"
                     /locus_tag="NBI57_001571"
     CDS             533..3238
                     /gene="acnA"
                     /locus_tag="NBI57_001571"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HCD1822511.1"
                     /translation="MAANFKEQSKKHFDLNGQSYTYYDLKAVEEQGITKVSNLPYSIR
                     VLLESLLRQEDDFVITDDHIKALSQFGKDGNEGEVPFKPSRVILQDFTGVPAVVDLAS
                     LRKAMDDVGGDITKINPEVPVDLVIDHSVQVDSYANPEALERNMKLEFERNYERYQFL
                     NWATKAFDNYNAVPPATGIVHQVNLEYLASVVHVRDVDGEKTAFPDTLVGTDSHTTMI
                     NGIGVLGWGVGGIEAEAGMLGQPSYFPIPEVIGVRLVNSLPQGATATDLALRVTQELR
                     KKGVVGKFVEFFGPGVQHLPLADRATIANMAPEYGATCGFFPVDDESLKYMKLTGRSD
                     EHIALVKEYLKQNHMFFDVEKEDPNYTDVIELDLSTVEASLSGPKRPQDLIFLSDMKS
                     SFENSVTAPAGNQGHGLDKSEFDKKAEINFKDGSKATMKTGDIAIAAITSCTNTSNPY
                     VMLGAGLVAKKAVEKGLKVPEYVKTSLAPGSKVVTGYLRDAGLQPYLDDLGFNLVGYG
                     CTTCIGNSGPLLPEIEKAIADEDLLVTSVLSGNRNFEGRIHPLVKANYLASPQLVVAY
                     ALAGTVDIDLQNEPIGKGNDGEDVYLKDIWPSIKEVSDTVDSVVTPELFIEEYNNVYN
                     NNELWNEIDVTDQPLYDFDPNSTYIQNPSFFQGLSKEPGTIVPLNGLRVMGKFGDSVT
                     TDHISPAGAIGKDTPAGKYLQDHQVPIREFNSYGSRRGNHEVMVRGTFANIRIKNQLA
                     PGTEGGFTTYWPTNEVMPIFDAAMKYKEDGTGLVVLAGNDYGMGSSRDWAAKGTNLLG
                     VKTVIAQSYERIHRSNLVMMGVLPLEFKKGESADSLGLDGTEEISVNIDENVQPHDYV
                     KVTAKKQDGDLVEFDAMVRFDSLVEMDYYRHGGILQMVLRNKLAQ"
     gene            3418..3885
                     /locus_tag="NBI57_001572"
     CDS             3418..3885
                     /locus_tag="NBI57_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HCD1822512.1"
                     /translation="MIYSITEIEARYAETDKMGVIYHGNYATWFEVARLDYISKLGFS
                     YADMEKQGIISPVTDLNVNYKKSIFYPEKVKVKTWVEKYSRLRSVYKYEIFNEKGELA
                     TTGSTELICIKEDTFKPIRLDRYFPDWHEAYSKVQTLNNEGKTVEIMDGIDSL"
     gene            complement(4092..4388)
                     /locus_tag="NBI57_001573"
     CDS             complement(4092..4388)
                     /locus_tag="NBI57_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HesB/YadR/YfhF family protein"
                     /protein_id="HCD1822513.1"
                     /translation="MQIELTDAAVTWFKNELELPENNKVLVFFVRYGGEFQLKQGFSP
                     AFTVEPKEDVDIGYEQQYDDLNVVVAEKDLWYFEDDHIIVNVVDHEDEISYSTK"
     gene            complement(4764..5372)
                     /gene="plsY"
                     /locus_tag="NBI57_001574"
     CDS             complement(4764..5372)
                     /gene="plsY"
                     /locus_tag="NBI57_001574"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HCD1822514.1"
                     /translation="MMIIVMLLLSYLIGAFPSGFVIGKLFFKKDIRQFGSGNTGATNS
                     FRVLGRPAGFLVTFLDIFKGFITVFFPLWLPVHADGPISTFFTNGLIVGLFAILGHVY
                     PVYLKFQGGKAVATSAGVVLGVNPILLLILAIIFFIVLKIFKYVSLASIVAAICCVIG
                     SLIIQDYILLVVSFLVSIILIIRHRSNIARIFRGEEPKIKWM"
     gene            5578..7569
                     /gene="parE"
                     /locus_tag="NBI57_001575"
     CDS             5578..7569
                     /gene="parE"
                     /locus_tag="NBI57_001575"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HCD1822515.1"
                     /translation="MNKQNNYSDDSIQVLEGLEAVRKRPGMYIGSTDKRGLHHLVYEI
                     VDNSVDEVLNGYGNEIDVTINKDGSISIEDNGRGMPTGIHKSGKPTVEVIFTVLHAGG
                     KFGQGGYKTSGGLHGVGASVVNALSEWLEVEIHRDGSIYHQSFKNGGSPSSGLVKKGK
                     TKKTGTKVTFKPDDTIFKASTSFNFDVLSERLQESAFLLKNLKITLNDLRSGKERQEH
                     YHYEEGIKEFVSYVNEGKEVLHDVATFSGEANGIEVDVAFQYNDQYSESILSFVNNVR
                     TKDGGTHEVGFKTAMTRVFNDYARRINELKTKDKNLDGNDIREGLTAVVSVRIPEELL
                     QFEGQTKSKLGTSEARSAVDSVVADKLPFYLEEKGQLSKSLVKKAIKAQQAREAARKA
                     REDARSGKKNKRKDTLLSGKLTPAQSKNTEKNELYLVEGDSAGGSAKLGRDRKFQAIL
                     PLRGKVINTEKARLEDIFKNEEINTIIHTIGAGVGTDFKIEDSNYNRVIIMTDADTDG
                     AHIQVLLLTFFFKYMKPLVQAGRVFIALPPLYKLEKGKGKTKRVEYAWTDEELNKLQK
                     ELGKGFTLQRYKGLGEMNPEQLWETTMNPETRTLIRVQVEDEVRSSKRVTTLMGDKVQ
                     PRREWIEKHVEFGMQEDQSILDNSEVQVLENDQFDEEEI"
     gene            7569..9971
                     /gene="parC"
                     /locus_tag="NBI57_001576"
     CDS             7569..9971
                     /gene="parC"
                     /locus_tag="NBI57_001576"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HCD1822516.1"
                     /translation="MSEIIQDLSLEDVLGDRFGRYSKYIIQERALPDVRDGLKPVQRR
                     ILYAMYSSGNTHDKNFRKSAKTVGDVIGQYHPHGDSSVYEAMVRLSQDWKLRHVLIEM
                     HGNNGSIDNDPPAAMRYTEAKLSLLAEELLRDINKETVSFIPNYDDTTLEPMVLPSRF
                     PNLLVNGSTGISAGYATDIPPHNLAEVIQATLKYIDNPDITVNQLMKYIKGPDFPTGG
                     IIQGIDGIKKAYESGKGRIIVRSKVEEETLRNGRKQLIITEIPYEVNKSSLVKRIDEL
                     RADKKVDGIVEVRDETDRTGLRIAIELKKDVNSESIKNYLYKNSDLQISYNFNMVAIS
                     DGRPKLMGIRQIIDSYLNHQIEVVANRTKFELDNAEKRMHIVEGLIKALSILDKVIEL
                     IRSSKNKRDAKENLIEVFEFTEEQAEAIVMLQLYRLTNTDIVALEGEHKELEALIKQL
                     RHILDNHDALLNVIKEELNEIKKKFKSERLSLIEAEIEEIKIDKEVMVPSEEVILSMT
                     RHGYIKRTSIRSFNASGVEDIGLKDGDSLLKHQEVNTQDTVLVFTNKGRYLFIPVHKL
                     ADIRWKELGQHVSQIVPIEEDEVVINVFNEKDFNTDAFYVFATQNGMIKKSTVPLFKT
                     TRFNKPLIATKVKENDDLISVMRFEKDQLITVITNKGMSLTYNTSELSDTGLRAAGVK
                     SINLKAEDFVVMTEGVSENDTILMATQRGSLKRISFKILQVAKRAQRGITLLKELKKN
                     PHRIVAAHVVTGEHSQYTLYSKSNEEHGLINDIHKSEQYTNGSFIVDTDDFGEVIDMY
                     IS"
BASE COUNT         3564 a         1463 c         1916 g         3049 t
ORIGIN      
        1 agttggggtg agggccccaa cacagaagct gacgaaaagt cggcttacaa taatgtgcaa
       61 gttggggatg ggccccaaca aagagaaatt ggattcccaa tttctacaga caatgcaagt
      121 tggcggggcc ccaacataga agctggcgga aagtcagttt acaataatgt gcaagttggg
      181 gtgggacgac gaaataaatt ttgcgaaaat atcatttctg tcccactccc atcaaaagaa
      241 tgacattgaa aagaaatatt aaagtttggc gccacaattt tctttctgct caatgaaata
      301 atacgtttga aaattgaatt gaatcattca cgaaattgat aattattctc aattaataaa
      361 aaatacttat tacaccttac ctgtatgata agttttgctt atatactctg attaaaaagt
      421 caaaacctaa attaagcgtt ttctaagcat gatgagttgt ttatcaataa atataataat
      481 acaatttaat caaggcatat aaatataaaa atgtatcaag ggggatcatt aaatggctgc
      541 aaattttaaa gagcaatcaa aaaaacattt tgacttgaat ggccaaagtt atacttacta
      601 tgatttaaaa gctgtagaag agcaaggtat tactaaagtt tccaatttac cttattcaat
      661 tcgtgttttg ttagaatctt tacttcgtca agaagatgat tttgtaatta cagacgatca
      721 tattaaagct ttaagtcagt ttggaaaaga tggaaatgaa ggcgaggtac catttaaacc
      781 ttctcgtgtt attttacaag atttcacagg tgtaccagcc gtagttgatt tagcttcttt
      841 acgtaaagca atggatgacg ttgggggaga tattactaaa attaatccag aagtaccggt
      901 ggatttagtt attgaccact cagttcaagt ggatagctat gcaaatccag aagctcttga
      961 acgtaatatg aaattagaat ttgaacgtaa ctatgaacgt tatcagtttt taaattgggc
     1021 aacgaaagca tttgataatt acaatgcagt tcctcctgca actggaatag ttcaccaagt
     1081 taacttagaa tatttagcaa gtgttgtaca tgttcgtgat gtagatggtg aaaaaactgc
     1141 atttccagat acattagttg gtactgattc acatacaaca atgataaatg gtattggcgt
     1201 actaggatgg ggtgttggtg gtattgaagc tgaagctgga atgcttggac aaccttctta
     1261 tttcccaatt ccagaggtta ttggtgtacg actagtaaat tcattaccac aaggcgcaac
     1321 agcaactgat ttagcgttaa gagtaactca agagctacgt aaaaaaggtg ttgttggtaa
     1381 atttgtggag ttctttggtc caggtgtaca acatttacca ctagcagacc gtgctacaat
     1441 tgcaaacatg gcaccagagt atggagcaac ttgcggattc ttcccagttg atgatgaatc
     1501 tcttaaatat atgaagttaa ctggtagatc agacgaacat atcgcgctag taaaagaata
     1561 tttgaaacaa aaccatatgt tctttgatgt tgagaaagaa gatcctaatt atacagatgt
     1621 tatcgaattg gatttatcaa cagttgaagc atcgctttca ggaccaaaac gtcctcaaga
     1681 tttaattttc ttaagtgata tgaaatcatc atttgaaaat tctgtaacag ctccagcagg
     1741 caaccaagga cacggtttag ataaaagtga atttgataag aaagctgaaa ttaactttaa
     1801 agatggatca aaagctacaa tgaaaacagg tgatattgca atagcagcaa ttacatcatg
     1861 tacaaataca tctaaccctt atgtaatgtt aggtgcaggt ttagttgcta aaaaagcagt
     1921 tgaaaaaggc ttgaaagttc ctgaatacgt taaaacttct ctagcaccag gatcaaaagt
     1981 tgttaccgga tatttaagag atgctggctt acaaccttat ttagatgatt taggcttcaa
     2041 cttggttggt tatggatgta caacttgtat cggtaattca ggtcctttat taccagaaat
     2101 tgaaaaagcg attgctgatg aggacctatt agtgacatct gtattatctg gtaaccgtaa
     2161 ctttgaaggt cgtatccatc ctcttgttaa agccaattac ctagcttcac cacagttagt
     2221 tgttgcttat gcattagctg gaacggttga tattgattta cagaatgaac ctattggtaa
     2281 aggtaatgac ggtgaagatg tatatttgaa agatatttgg ccatcaatta aagaagtttc
     2341 agataccgtt gatagtgttg taacacctga attatttatt gaagaatata ataacgtata
     2401 caataacaac gaattatgga atgagattga tgtaactgat caacctctat atgactttga
     2461 tcctaattca acatacattc aaaatccatc attcttccaa ggattatcta aagaaccggg
     2521 tacgattgtt ccattaaatg gtttacgtgt tatgggtaaa ttcggtgatt ctgtgacaac
     2581 tgaccacatc tctccagcag gtgcaattgg taaagatacg ccagctggta aatatttaca
     2641 agatcatcaa gtgcctattc gtgaatttaa ttcatatggt tcaagacgtg gtaatcacga
     2701 agtaatggtt cgaggtacgt ttgctaatat acgtattaaa aaccaattag cgccaggtac
     2761 tgaaggtggt tttacaactt attggccaac aaatgaagta atgcctatct ttgatgctgc
     2821 aatgaaatat aaagaagatg gtacaggttt agttgtatta gctggtaacg attatggtat
     2881 gggttcatct cgtgactggg cagcaaaagg tacaaactta ttaggtgtta aaacagttat
     2941 tgcacaaagt tatgaacgta tccatcgttc aaatttagtt atgatgggtg tattaccatt
     3001 agagtttaaa aaaggtgaat cagctgattc tcttggtcta gatggtacag aagaaatttc
     3061 tgttaatatt gatgaaaatg ttcaaccaca tgactacgtc aaagttactg ctaagaagca
     3121 agatggtgat ttggtagaat ttgacgctat ggttcgtttt gactcacttg ttgaaatgga
     3181 ttactatcgt cacggtggaa ttttacaaat ggttttaaga aataaattag cgcaataaaa
     3241 aatagatatc acagtaaaat ttcaatcggt atttgaggca ctttgatagt gctcaaaaat
     3301 acttctataa actttatgat ataataggtc ggttcaaaac ctttgtgttc ttggccggcc
     3361 tcttcttgta ttttatgtta gaatgagata taaagttaaa ctgaaagaag gcttaaaatg
     3421 atatatagta ttacagaaat agaagcgcgt tatgctgaaa ctgataagat gggtgtaatt
     3481 tatcacggga attatgcaac ttggtttgaa gttgcgcggt tggattatat atcgaagtta
     3541 ggttttagtt atgctgatat ggaaaaacaa ggaatcattt cacctgtgac tgacctcaat
     3601 gtcaattata aaaagtctat tttttatcca gaaaaagtta aagttaaaac ttgggttgaa
     3661 aaatattcga gattacgttc agtgtataaa tatgagattt ttaatgaaaa aggtgaactt
     3721 gcaactacag gttccacaga attgatttgc attaaagaag atacttttaa gcctatacgg
     3781 ttggatcgtt atttcccaga ttggcatgaa gcttatagta aagtgcaaac gctcaataat
     3841 gaagggaaaa cagtagagat aatggatggt attgattctt tataagttac tgtaaatgtt
     3901 tggagaggca atattaacaa ttaatctata agaaatagcc aatacgttaa cattaatgta
     3961 attagcgtga agataagcta atatatagca aaaacgaact tgttcataca acatcagatg
     4021 tgctaaacca aagtacaact tccgaggttc aggacacatc agaatgtgta aacaagttcg
     4081 ttttatttta gttattttgt ggaataagaa atttcatctt cgtgatcaac tacatttaca
     4141 ataatgtggt catcttcaaa gtaccacaaa tctttttccg ctacgacaac atttaaatcg
     4201 tcatattgtt gttcatagcc aatatcaaca tcttcctttg gttcaactgt aaaagcagga
     4261 ctaaatcctt gcttgagttg gaattcgcca ccatatctta caaaaaacac gagcacttta
     4321 ttattttcag gcaactcaag ttcattttta aaccaagtta ctgctgcatc agtaagttct
     4381 atttgcataa caatgacctc ctatatttat tacagaatgg gagtgggcca acttgtatta
     4441 tttgtggaat ttcttatcga aattctctgt gttggagccc accccaacct gctcattatt
     4501 gtaagctgaa tttccataag cttctatgtt gggacccccg accagaattg aaaaaaccta
     4561 gttataagcg catttcgttc ggttaactac agctaatata acattgtgga gcctatgaca
     4621 ttgatttatg tctcggtctc aaatgttctt gtaaaaaaac taagtatagt tgaattttaa
     4681 gttaaaaaaa ttgtttttgc aaaaataaaa taatccatca actgcaaaaa aatatgcaat
     4741 gatggactaa tgtcaaaaaa ttgttacatc cattttattt taggttcttc gcctctaaaa
     4801 atccttgcga tattagagcg atgtctaatt atcaatatga ttgaaactaa gaaactaacg
     4861 actaataaaa tatagtcttg aatgataagc gagccaatca cacagcaaat tgctgcaacg
     4921 atacttgcta aagaaacata tttaaaaatc ttcaatacaa taaagaagat aattgcaagt
     4981 attagtaaaa gtatcggatt gactcccaag acgacacctg cactagttgc aacagctttg
     5041 ccaccttgga attttaaata aacaggataa acgtgtccaa gtatagcgaa taagccaaca
     5101 attaaaccat ttgtaaaaaa agtactaata gggccatctg cgtgaactgg taaccataaa
     5161 gggaagaaaa cagttatgaa ccctttgaaa atatctagaa atgttaccaa gaatcctgca
     5221 ggacgaccta atactctaaa gctattagta gcgccagtat taccactacc aaattgtcta
     5281 atatcttttt tgaaaaataa ttttccaatt acgaatccac ttgggaaagc gccgataaga
     5341 taacttagta gtaacatgac gattatcatc ataaatatta cacatccttt aatatcttag
     5401 gactatttta tcatattcat ttaaattacg gctaaaaatt ttaaaaacgg ggattaatat
     5461 atggaattaa gctatgaaag ttaattgata cttgcatttt acgctgattt atataagaat
     5521 aactattgta tagttttaaa aacgaacgta cgtttgcagg aggcgaaatc attggcaatg
     5581 aataaacaaa ataattattc agatgattca atacaggttt tagaggggtt agaagcagtt
     5641 cgtaaaagac ctggtatgta tattggatca actgataaac ggggattaca tcatctagta
     5701 tatgaaattg tcgataactc cgtcgatgaa gtattgaatg gttacggtaa cgaaatagat
     5761 gtaacaatta ataaagatgg tagtatttct atagaagata atggacgtgg tatgccaaca
     5821 ggtatacata aatcaggtaa accgacagtc gaagttatct ttactgtttt acatgcagga
     5881 ggtaaatttg gacaaggcgg ctataaaact tcaggtggtc ttcacggtgt tggtgcttca
     5941 gttgtaaatg cattgagtga atggcttgaa gttgaaatcc atcgagatgg tagtatatat
     6001 catcaaagtt ttaaaaacgg tggttcgcca tcttctggtt tagtgaaaaa aggtaaaact
     6061 aagaaaacag gtaccaaagt aacatttaaa cctgatgaca caatttttaa agcatctaca
     6121 tcatttaatt ttgatgtttt aagcgaacga ctacaagagt ctgcgttctt attgaaaaat
     6181 ttaaaaataa cgcttaatga tttacgcagt ggtaaagagc gtcaagagca ttaccattat
     6241 gaagaaggaa tcaaagagtt tgttagttat gtcaatgaag gaaaagaagt tttgcatgac
     6301 gtggctacat tttcaggtga agcaaatggt atagaggtag acgtagcttt ccaatataat
     6361 gatcaatatt cagaaagtat tttaagtttt gtaaataatg tacgtactaa agatggtggt
     6421 acacatgaag ttggttttaa aacagcaatg acacgtgtat ttaatgatta tgcacgtcgt
     6481 attaatgaac ttaaaacaaa agataaaaac ttagatggta atgatattcg tgaaggttta
     6541 acagctgttg tgtctgttcg tattccagaa gaattattac aatttgaagg acaaacgaaa
     6601 tctaaattgg gtacttctga agctagaagt gctgttgatt cagttgttgc agacaaattg
     6661 ccattctatt tagaagaaaa aggacaattg tctaaatcac ttgtgaaaaa agcgattaaa
     6721 gcacaacaag caagggaagc tgcacgtaaa gctcgtgaag atgctcgttc aggtaagaaa
     6781 aacaagcgta aagacacttt gctatctggt aaattaacac ctgcacaaag taaaaacact
     6841 gaaaaaaatg aattgtattt agtcgaaggt gattctgcgg gaggttcagc aaaacttgga
     6901 cgagaccgca aattccaagc gatattacca ttacgtggta aggtaattaa tacagagaaa
     6961 gcacgtctag aagatatttt taaaaatgaa gaaattaata caattatcca cacaatcggg
     7021 gcaggcgttg gtactgactt taaaattgaa gatagtaatt ataatcgtgt aattattatg
     7081 actgatgctg atactgatgg tgcgcatatt caagtgctat tgttaacatt cttcttcaaa
     7141 tatatgaaac cgcttgttca agcaggtcgt gtatttattg ctttacctcc actttataaa
     7201 ttggaaaaag gtaaaggcaa aacaaagcga gttgaatacg cttggacaga cgaagagctt
     7261 aataaattgc aaaaagaact tggtaaaggc ttcacgttac aacgttacaa aggtttgggt
     7321 gagatgaacc ctgaacaatt atgggaaacg acgatgaacc cagaaacacg aactttaatt
     7381 cgtgtacaag ttgaagatga agtgcgttca tctaaacgtg taacaacatt aatgggtgac
     7441 aaagtacaac ctagacgtga atggattgaa aagcatgttg agtttggtat gcaagaggac
     7501 caaagtattt tagataattc tgaagtacaa gtgcttgaaa atgatcaatt tgatgaggag
     7561 gaaatctagt gagtgaaata attcaagatt tatcacttga agatgtttta ggtgatcgct
     7621 ttggaagata tagtaaatat attattcaag agcgtgcatt gccagatgtt cgtgatggtt
     7681 taaaaccagt acaacgtcgt attttatatg caatgtattc aagtggtaat acacacgata
     7741 aaaatttccg taaaagtgcg aaaacagtcg gtgatgttat tggtcaatat catccacatg
     7801 gagactcctc agtgtacgaa gcaatggtcc gtttaagtca agactggaag ttacgacatg
     7861 tcttaataga aatgcatggt aataatggta gtatcgataa tgatccgcca gcggcaatgc
     7921 gttacactga agctaagtta agcttactag ctgaagagtt attacgtgat attaataaag
     7981 agacagtttc tttcattcca aactatgatg atacgacact cgaaccaatg gtattgccat
     8041 caagatttcc taacttacta gtgaatggtt ctacaggtat atctgcaggt tacgcgacag
     8101 atataccacc acataattta gctgaagtga ttcaagcaac acttaaatat attgataatc
     8161 cggatattac agtcaatcaa ttaatgaaat atattaaagg tcctgatttt ccaactggtg
     8221 gtattattca aggtattgat ggtattaaaa aagcttatga atcaggtaaa ggtagaatta
     8281 tagttcgttc taaagttgaa gaagaaactt tacgcaatgg acgtaaacag ttaattatta
     8341 ctgaaattcc atatgaagtg aacaaaagta gcttagtaaa acgtatcgat gaattacgtg
     8401 ctgacaaaaa agtcgatggt atcgttgaag tacgtgatga aactgataga actggtttac
     8461 gaatagcaat tgaattgaaa aaagatgtga acagtgaatc aatcaaaaat tatctttata
     8521 aaaactctga tttacagatt tcatataatt tcaacatggt cgctattagt gatggtcgtc
     8581 caaaattgat gggtattcgt caaattatag atagttattt gaatcatcaa attgaggttg
     8641 ttgcaaatag aacgaagttt gaattagata atgctgaaaa acgtatgcat atcgttgaag
     8701 gtttgattaa agcgttgtca attttagata aagtaattga attgattcgt agctctaaaa
     8761 acaagcgtga cgctaaagaa aaccttatcg aagtattcga gttcacagaa gaacaggctg
     8821 aagcaattgt aatgttacag ttatatcgtt taacaaacac tgacatagtt gcgcttgaag
     8881 gtgaacataa agaacttgaa gcattaatca aacaattacg tcatattctt gataaccatg
     8941 atgcattatt gaatgtcata aaagaagaat tgaatgaaat taaaaagaaa ttcaaatctg
     9001 aacgactgtc tttaattgaa gcagaaattg aagaaattaa aattgacaaa gaagttatgg
     9061 tgcctagtga agaagttatt ttaagtatga cacgtcatgg atatattaaa cgtacttcta
     9121 ttcgtagctt taatgctagc ggtgttgagg atattggttt aaaagatggt gacagtttac
     9181 ttaaacatca agaagtaaat acgcaagata ccgtactagt atttacaaat aaaggtcgtt
     9241 atctatttat accggttcat aaattagcag atattcgttg gaaagaattg gggcaacatg
     9301 tatcacaaat agttcctatc gaagaagatg aagtggttat taatgtcttt aatgaaaagg
     9361 actttaatac agatgcattt tatgtttttg cgactcaaaa tggcatgatt aagaaaagta
     9421 cagtgcctct atttaaaaca acgcgtttta ataaaccttt aattgctact aaagttaaag
     9481 aaaatgatga tttgattagt gttatgcgct ttgaaaaaga tcaattaatt accgtcatta
     9541 ctaataaagg tatgtcatta acgtataata caagtgaact atcagatacc ggattaaggg
     9601 cagctggtgt taaatcaata aatcttaaag ctgaagattt cgttgttatg acagaaggtg
     9661 tttctgaaaa tgatactata ttgatggcca cacaacgcgg ctcgttaaaa cgtattagtt
     9721 ttaaaatctt acaagttgct aaaagagcac aacgtggaat aactttatta aaagaattaa
     9781 agaaaaatcc acatcgtatt gtagctgcac atgtagtgac aggtgaacat agtcaatata
     9841 cattatattc aaaatcaaat gaagaacatg gtttaattaa tgatattcat aaatctgaac
     9901 aatatacaaa tggctcattc attgtagata cagatgattt tggtgaagta atagacatgt
     9961 atattagcta aaaactatat gcaatcacga aa
//
LOCUS       DAHHSC010000087         9808 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.088, whole genome shotgun sequence.
ACCESSION   DAHHSC010000087 DAHHSC010000000
VERSION     DAHHSC010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 9808)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9808)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9808
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.088"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            17..514
                     /locus_tag="NBI57_001577"
     CDS             17..514
                     /locus_tag="NBI57_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCD1822517.1"
                     /translation="MINTERLNLMIPSSSHLIELYNICSHPQANIYTPKGLHNSKLDT
                     QRWIEKWQNHWQQYQFGYFVLVKKIDCSVIGICGYEYRQLKQETVLNLFYKLHPSFEG
                     QGYACEAITAITNFVNYIDQETVKVIRTNKCNQRSINLAERLKFKRDKAMDDIINQGD
                     IVFYK"
     gene            632..1756
                     /locus_tag="NBI57_001578"
     CDS             632..1756
                     /locus_tag="NBI57_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HCD1822518.1"
                     /translation="MHKKYLLLFFLLLSVALLYSCSKQNEKAQNKSVNPNEYAKHKSK
                     MDEHWETYHGQVYHVFYHPLITDPKVAFTGDTHQAKGNNDWMITVSEFKKSLNELYKN
                     NYIIVNPHDVYDLSSHHVKAKKIKLPKGKKPLIISIDDMNYYSYMRHHGYADRLVLDK
                     NKHVVSETTDKKGHATYSDDNDIVPILNKFVKEHPDFSLNGEKGVVALTGYEGVLGYR
                     TNELTSKDYTKNKKAAEEVVRAMKRDGWSFASHSYGHINFEKTSLEGIKRDTKRWKDE
                     VEPIVGKTDMFVFPHGAQDRHTQAYDYLVDEAEFKFIAGVGPNNFTDISATNVYQDRV
                     AIDGLNLFEFKYKLKPFFNPENVYSKQDRQFFKGNRDYEE"
     gene            2060..2878
                     /locus_tag="NBI57_001579"
     CDS             2060..2878
                     /locus_tag="NBI57_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HCD1822519.1"
                     /translation="MNNSEQLIALKESETAFLKYFNKADYELVDFSVVEKLDWKQLNH
                     EDLQQMGERNFWQHEHQIYALRNDFTDQLLRYYSMYPTAATKVAYTGLIIRNNEAAVQ
                     VGLENYAPSLANVQQSLKLFIQFIQQQLRDNVHFVVLGHYQLLDALLDKSLQTPDILS
                     MIEERNLSGLVTYLSTEHPIVQILKENTQQQLNVLEHYIPNDHPALVELKIWERWLHT
                     QGYKDIHLDITAQPPRSYYTGLFIQCHFAENESRVLTGGYYKGSIEGFGLGLTL"
     gene            2896..3510
                     /locus_tag="NBI57_001580"
     CDS             2896..3510
                     /locus_tag="NBI57_001580"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HCD1822520.1"
                     /translation="MLRIAIAKGRLMDSLINYLDVIEYTTLSETLKNRERQLLLSVDN
                     IECILVKGSDVPIYVEQGMADIGIVGSDILDERQYNVNNLLNMPFGACHFAVAAKPET
                     TNYRKIATSYVHTAETYFKSKGIDVELIKLNGSVELACVVDMVDGIVDIVQTGTTLKA
                     NGLVEKQHISDINARLITNKAAYFKKSQLIEQFIRSLEVSIANA"
     gene            3503..4753
                     /locus_tag="NBI57_001581"
     CDS             3503..4753
                     /locus_tag="NBI57_001581"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HCD1822521.1"
                     /translation="MLNAQQFLNQFSLEAPLDESLYPIIRDICQEVKVHGDKALKMYN
                     LTFDHTKTDHLEISHEQIKAAFDTLDEKTKQALQQSYERIKAYQESIKQTNQQLEESV
                     ECYEIYHPLESVGIYVPGGKASYPSTVLMTATLAQVAGVENIVVVTPPQPNGVSQEVL
                     AACYITQVNQVFQVGGAQSIAALTYGTETIPKVDKIVGPGNQFVAYAKKYLFGQVGID
                     QIAGPTEIALIIDDTADLDAIVYDVFAQAEHDELARTYVIGEDAQVLKDLESRIAKAL
                     PNVDRYDIVSKSIANQHYLIHASNFDEACHVMNTIAPEHASIQTVNPQPYIEKVKYVG
                     ALFIGHYSPEVIGDYVAGPSHVLPTNRTARFTNGLSVNDFLTRNTVIHLSKDTFEQIA
                     DSAQHIAHVEALYNHQQSILIRQS"
     gene            4769..5782
                     /locus_tag="NBI57_001582"
     CDS             4769..5782
                     /locus_tag="NBI57_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate aminotransferase family
                     protein"
                     /protein_id="HCD1822522.1"
                     /translation="MIYIDKNESPVTPLDEKTMTSIISATPYNLYPDAAYEQFKEAYA
                     KFYGLSPEQIIAGNGSDELIQKLMLIMPEGPALTLNPDFFMYQAYAAQVNREIAFVDA
                     GSDLTFDLETILTKIDEVQPSFFIMSNPHNPSGKQFDTAFLTAIADKMKALNGYFVID
                     EAYLDYGTAYDVELAPHILRMRTLSKAFGIAGLRLGVLISTAGTIKHIQKIEHPYPLN
                     VFTLNIATYIFRHREETRVFLTMQRQLAEQLKQIFDTHVADKMSVFPSNANFVLTKGS
                     AAHQLGQYVYEQGFKPRFYDEPVMKGYVRYSIATASQLKQLEEIVKEWSAKYDLSKTT
                     KHS"
     gene            5751..6329
                     /gene="hisB"
                     /locus_tag="NBI57_001583"
     CDS             5751..6329
                     /gene="hisB"
                     /locus_tag="NBI57_001583"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HCD1822523.1"
                     /translation="MIYQKQRNTAETQLNISISDDQSPSHINTGVGFLNHMLTLFTFH
                     SGLSLNIEAQGDIDVDDHHVTEDIGIVIGQLLLEMIKDKKHFVRYGTMYIPMDETLAR
                     VVVDISGRPYLSFNASLSKEKVGTFDTELVEEFFRAVVINARLTTHIDLIRGGNTHHE
                     IEAIFKAFSRALGIALTATDDQRVPSSKGVIE"
     gene            6326..6904
                     /gene="hisH"
                     /locus_tag="NBI57_001584"
     CDS             6326..6904
                     /gene="hisH"
                     /locus_tag="NBI57_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HCD1822524.1"
                     /translation="MIVIVDYGLGNISNVKRAIEHLGYEVVVSNTSKIIDQAETIILP
                     GVGHFKDAMSEIKRLNLNAILAKNTDKKMIGICLGMQLMYEHSDEGDASGLGFIPGNI
                     SRIQTEYPVPHLGWNNLVSKHPMLNQDVYFVHSYQAPMSENVIAYAQYGADIPAIVQF
                     NNYIGIQFHPEKSGTYGLQILRQAIQGGFIND"
     gene            6897..7601
                     /locus_tag="NBI57_001585"
     CDS             6897..7601
                     /locus_tag="NBI57_001585"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-((5-
                     phosphoribosylamino)methylideneamino)imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HCD1822525.1"
                     /translation="MIELWPAIDLIGSTSVRLTEGKYDSEEKMSRSAEESIAYYSQFE
                     CVNRIHIVDLIGAKAQYAREFDYIKSLRRLTTKDIEVGGGIRTKSQIMDYFAAGINYC
                     IVGTKGIQDTDWLKEMAHTFPGRIYLSVDAYGEDIKVNGWEEDTELNLFSFVRQLSDI
                     PLGGIIYTDIAKDGKMSGPNFELTGQLVKATTIPVIASGGIRHQQDIQRLASLNVHAA
                     IIGKAAHQASFWEGLE"
     gene            7598..8356
                     /gene="hisF"
                     /locus_tag="NBI57_001586"
     CDS             7598..8356
                     /gene="hisF"
                     /locus_tag="NBI57_001586"
                     /EC_number="4.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000592599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HCD1822526.1"
                     /translation="MIKKRIIPCLDVKDGRVVKGIQFKGLRDIGNPVDLAIYYNEAGA
                     DELVFLDISKTEEGHSLMLEVIEQTASRLFIPLTVGGGIQSLDDITQLLNHGADKVSL
                     NSSALKNPQLIKQASDKFGRQCICIAIDSYYDPERKAHYCCTHGGKKMTNIKVYDWVQ
                     QVEQLGAGELLVTSMGHDGMKQGFDIEHLAKIKSLVNIPIIASGGGGNAQHFVELFNQ
                     TDVSAGLAASILHDRETMVQSIKEVIRQGGIAVR"
     gene            8353..8985
                     /locus_tag="NBI57_001587"
     CDS             8353..8985
                     /locus_tag="NBI57_001587"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HCD1822527.1"
                     /translation="MTKYKIDFSKGLVPAILQDNQTKQVLMLGYMNQEAFDKTIEDGV
                     VCFYSRSKQRLWTKGETSGHTQLVKDIHVDCDNDTILIDVIPNGPTCHTGSQSCFNTE
                     VPFSVQTLAQTVQDSAQSNNEKSYTKYLLTEGIEKITKKYGEEAFEVVIEAIKGDKKA
                     FVSEVADELYHLFVLMHALGVDFSEIEAELARRHHKRNNFKGERQNIEQW"
     gene            9178..9357
                     /locus_tag="NBI57_001588"
     CDS             9178..9357
                     /locus_tag="NBI57_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000837587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1822528.1"
                     /translation="MKSKLSNSGIDVTVKDVEKYMNRYNEVMMGKNGEKAKELCLSLL
                     PINIIVVFTFFVFIL"
BASE COUNT         3555 a         1435 c         1915 g         2903 t
ORIGIN      
        1 aggagggaga catttaatga taaatactga aagattaaat ttaatgattc caagttcctc
       61 gcatttaatt gaactttata atatttgtag tcatccacaa gcaaatatat acactcccaa
      121 aggtttacat aattccaaat tagacacaca acggtggatt gaaaaatggc aaaaccattg
      181 gcaacaatat caatttggtt actttgtatt ggtgaaaaaa atagattgta gtgttattgg
      241 tatttgtgga tatgaatatc gacaattaaa gcaagaaaca gtactaaatt tattttataa
      301 attacatcca agttttgaag gacaagggta cgcatgtgag gctattacag caatcacaaa
      361 ttttgtgaat tatatcgatc aagaaacagt aaaagttatc aggacaaata agtgtaacca
      421 acgttcaata aatttagcag aaaggcttaa attcaagcga gacaaagcta tggacgacat
      481 tatcaatcaa ggagatattg tgttttataa ataaaatact atgacattat ctaaaaaata
      541 aaattaaaat cccaacagtt tcttaaaaag ttatgagcca cataataaaa atgttataat
      601 tgtgcataat aaaacatagg gagaatgttt aatgcataaa aaatatttat tgctattttt
      661 tctgttatta tcagttgcat tgctatatag ctgttccaag caaaatgaga aagctcaaaa
      721 taaatctgtt aatccaaatg agtatgcaaa gcataaatcg aaaatggatg aacattggga
      781 aacatatcac ggccaagttt atcatgtgtt ttatcaccca ttaattacag acccaaaagt
      841 tgcgtttaca ggagatacac accaagctaa gggcaacaat gattggatga ttactgtaag
      901 tgaatttaaa aaatcattga acgaattata taaaaataat tacatcatcg ttaatccaca
      961 tgatgtatac gacttatcat ctcatcatgt aaaagccaaa aaaataaaat tgccgaaagg
     1021 taaaaagcca ctaatcatct caattgatga tatgaattac tatagttaca tgagacacca
     1081 tggttatgct gaccgtttag tactcgataa aaataaacat gttgtttctg aaactacaga
     1141 caaaaaaggt catgctactt attctgatga caatgatatc gtgccaattt taaataaatt
     1201 tgtaaaagag catccagatt tttctttaaa tggtgaaaaa ggtgttgttg ctttaaccgg
     1261 ttatgagggc gtattaggtt atcgcacgaa tgaattaacg agtaaagatt atacaaagaa
     1321 taaaaaggca gctgaggaag ttgttcgcgc aatgaaacgg gatggatggt catttgcgag
     1381 tcattcatat ggtcacatta attttgaaaa aacatcttta gaaggtatta aacgtgatac
     1441 taaaagatgg aaagatgaag ttgaaccaat tgtaggtaaa acagatatgt ttgtatttcc
     1501 acatggcgca caggatagac atacacaagc gtatgattat ttggtagatg aagcagagtt
     1561 taagtttatt gcaggtgtcg gtccaaacaa ttttacagat atatcagcaa cgaacgttta
     1621 ccaagatagg gttgcgattg atggtctaaa cttatttgaa tttaaatata aactcaaacc
     1681 gttttttaat cctgaaaatg tatatagtaa acaagataga caattcttta aaggtaatcg
     1741 ggattatgaa gaatagttaa atttaataaa aagatagaaa gaaaataact ttacataata
     1801 aggcaagcac aatatgtgca agaagtacaa cttcaatata aaaatcttgg tttagcattt
     1861 ttataggcac aaatgtcttg cctttttctt ttgtaaaaat ttaaaaacaa tacaccgaat
     1921 tttctgaaaa aggttgtaat gtattgctaa aaatgataag ttattaatca tattacactt
     1981 tagtgtggta tagaagtaaa gtaaacaacg aaaataccaa gtgattttaa atacagtatt
     2041 gaaagtaaag ggtggtcgag tgaataattc agaacaatta attgcgttaa aagaatcgga
     2101 aacagcattt ttaaaatatt ttaataaagc ggattatgag ttagttgatt ttagtgtggt
     2161 tgaaaagttg gactggaaac aattgaacca tgaggatttg cagcagatgg gtgagcgaaa
     2221 tttttggcaa catgagcatc aaatttatgc acttcgaaat gattttacag atcaattatt
     2281 gcgttattac tcaatgtatc caactgcagc aacaaaagtg gcatacacag gtttaatcat
     2341 tcggaataat gaagcggctg tacaagtggg tttggaaaac tatgcaccat cattagcgaa
     2401 tgtacaacag agcttaaagc tatttattca gtttattcaa cagcagttga gagacaacgt
     2461 gcattttgtt gtattgggac attatcaatt gcttgatgcc ttactcgata aatcgttaca
     2521 aacaccagac atactatcaa tgattgagga acgtaattta tcgggactgg ttacatattt
     2581 gtcgactgaa catccaatcg tgcaaatctt gaaagagaat acgcaacaac aattaaatgt
     2641 attggaacac tatattccaa atgaccatcc agcactcgtt gaattaaaaa tatgggaacg
     2701 ttggttacat acacaaggtt acaaagacat ccatttagat attactgcgc agccacctag
     2761 atcctattac acaggcttat ttattcaatg tcattttgct gaaaatgaat ctcgagtttt
     2821 aacaggtggt tattacaaag gaagcatcga agggtttgga ttaggattaa cactttaagt
     2881 aagggagtat gcacaatgtt aagaatcgcc atagccaaag gacgtctaat ggatagttta
     2941 attaactatt tagatgtaat tgaatatacg acattatcag aaacattaaa aaatagagaa
     3001 cgccaattat tattaagtgt agataatatt gaatgcattt tagtaaaagg aagtgacgtg
     3061 ccaatctatg tggaacaagg aatggccgac ataggcattg ttggtagcga catattagat
     3121 gagcgccaat ataatgttaa taatttgttg aatatgcctt ttggagcatg tcattttgcg
     3181 gttgcagcga aacctgaaac gaccaattat cgtaaaatcg caacgagtta tgttcatact
     3241 gctgaaacat attttaaatc aaaaggtatt gatgtcgaat tgattaaatt gaatggttct
     3301 gttgaattag cttgtgtcgt agatatggta gacggaattg tcgacatcgt tcaaacaggt
     3361 actacgctaa aagcgaacgg actggttgaa aagcaacata ttagtgatat caatgcaaga
     3421 ttaataacta ataaagcagc ttattttaaa aaatcacaat taatagagca atttattcgc
     3481 tctttggagg tgtctattgc caatgcttaa tgcacaacaa tttttaaatc aattttcatt
     3541 agaagcacca ttagatgagt cattgtatcc aattattcgc gatatttgtc aggaagttaa
     3601 agttcatgga gataaggctt tgaaaatgta taatctaaca ttcgatcata cgaaaacaga
     3661 tcatttagaa attagtcatg aacaaattaa agcagcattt gacacattag atgaaaaaac
     3721 aaaacaagca ttacaacaaa gttatgaaag aattaaagca tatcaagaaa gtattaagca
     3781 gacgaatcaa cagttagaag aatcagtgga gtgttatgaa atataccatc cactagaaag
     3841 tgtgggtatt tatgtgcctg gtggcaaagc aagttatcca tcaacggttc taatgacagc
     3901 gactttagca caagtagcgg gtgtagaaaa tattgttgtt gtgacaccac ctcaacctaa
     3961 cggagtatcc caagaggtat tagctgcatg ttatattacg caagttaatc aagtgtttca
     4021 agttggtggt gctcaaagta ttgctgcatt gacttatgga acagaaacga tacctaaagt
     4081 tgataagatt gtaggtccag gtaaccaatt tgttgcatat gccaaaaaat atttatttgg
     4141 acaggtaggt attgatcaaa ttgcaggacc aacagaaata gcactgatta ttgacgacac
     4201 cgcagattta gatgccatcg tatatgatgt atttgcgcaa gcagaacatg atgaattagc
     4261 acgtacatat gtcattggtg aagatgcgca agtccttaaa gatttagaat cacgtattgc
     4321 taaagcattg cctaatgtgg acagatacga cattgtttct aaaagtatcg ctaatcaaca
     4381 ctaccttatc catgctagta attttgatga agcatgccat gtcatgaata caatcgcgcc
     4441 tgaacatgcg tcgattcaaa cagtaaatcc tcaaccatat attgaaaaag tgaaatatgt
     4501 gggtgcattg tttattggac attattcgcc agaggtcata ggagattacg ttgcaggtcc
     4561 aagtcatgta ttacctacaa atagaacagc tagatttacc aatgggttat cggtcaatga
     4621 tttcttaaca cggaacacgg tcatccattt atcaaaagat acgtttgaac aaattgctga
     4681 ttcagcacaa catattgctc atgttgaagc attatacaat caccagcagt ctattttaat
     4741 acgtcagtct taggggagtg taattgaaat gatttatatt gataaaaatg aaagtccagt
     4801 tacgccgttg gatgaaaaaa caatgacgtc tattattagt gcaacgccat ataatttata
     4861 tcctgatgca gcatatgaac aattcaagga agcttatgct aagttttacg gattatcgcc
     4921 tgaacaaatt attgcaggaa atggatctga tgaattgatt caaaagttaa tgctgatcat
     4981 gccagaaggt ccggcattaa cgctaaatcc tgattttttt atgtatcaag catatgcggc
     5041 acaagtaaat cgtgaaattg catttgtaga tgcaggatca gatttaacgt ttgatttgga
     5101 aaccatttta acgaaaatcg atgaagtaca accatcattt tttattatga gtaatccaca
     5161 taacccttca ggcaagcaat ttgatacggc atttttaaca gctattgcag ataagatgaa
     5221 agcattaaac ggatactttg tcattgatga agcatattta gattatggta cggcatatga
     5281 cgtggaactg gcaccacaca tcttaagaat gcgtacatta tcaaaggcgt ttggaattgc
     5341 cggcttaaga ttaggtgtct taattagtac tgctggaacg ataaagcata ttcaaaaaat
     5401 agaacatcca tatccattaa atgtatttac gctaaatatt gcgacttata tttttagaca
     5461 tagagaagag acaagagtat ttttaacgat gcaacgacag ttagctgagc agttaaaaca
     5521 aatatttgat acacatgttg cagataaaat gtcagtattc ccatcaaatg ccaattttgt
     5581 acttactaaa ggttcagcag cgcaccaatt aggacaatac gtatatgaac aaggatttaa
     5641 acctcgcttt tatgatgagc cggtgatgaa gggctatgta agatactcaa ttgcaacagc
     5701 atcgcagtta aagcaattag aagaaattgt taaagaatgg agcgcaaaat atgatttatc
     5761 aaaaacaacg aaacacagct gaaacacaac taaatatttc aatatcagat gaccagtcac
     5821 catcgcatat taatacaggt gtgggctttt taaatcatat gttaacattg tttacatttc
     5881 atagcggtct gtcattaaac attgaggcac aaggtgatat tgacgtagat gatcaccatg
     5941 taactgaaga tatcggcatt gtcattggcc aattgttact tgaaatgatt aaagataaaa
     6001 agcatttcgt tcgttatgga acgatgtaca ttccaatgga tgaaacatta gcacgtgtcg
     6061 ttgtggatat aagtgggcgc ccatacctat cattcaatgc atcattaagt aaagaaaaag
     6121 ttggtacgtt tgatacggag ttagtagaag aattttttag agcggtcgta atcaatgcaa
     6181 gattaacaac gcatattgat ttaattcgtg gaggtaatac acaccatgaa attgaagcta
     6241 tattcaaagc gttttcccgt gcattaggca tagcgctaac tgcaactgat gatcagcgtg
     6301 tgccgtcatc gaaaggtgtg attgaatgat tgtcatcgtt gattatggat tagggaatat
     6361 tagtaatgta aaacgcgcta ttgaacattt agggtatgag gtggttgtct caaatacctc
     6421 aaaaataatc gatcaagcag aaacaatcat attgcccggt gtcggccatt ttaaagatgc
     6481 gatgtcagag ataaaacgat taaatctcaa tgcaatattg gctaagaata ctgataagaa
     6541 gatgattggt atttgtttag gcatgcaatt aatgtatgag catagtgatg aaggcgatgc
     6601 atctggatta gggtttatcc caggaaatat ttcgcgtatc caaacagaat acccagtgcc
     6661 gcacttaggt tggaataatt tagtgagtaa gcatcctatg ttaaatcaag atgtttactt
     6721 cgtacattct taccaagcgc cgatgtcaga aaatgtcatt gcatatgcgc agtatggggc
     6781 tgatattccg gcaattgttc aatttaacaa ttatattggt attcaattcc atcctgaaaa
     6841 aagcggtaca tatgggttac aaattttgcg tcaggcaata caagggggat ttataaatga
     6901 ttgaattatg gccagcgatt gatttgattg ggtcaacaag tgtgaggtta acagagggta
     6961 aatatgatag tgaagaaaaa atgtcgcgtt cggctgaaga aagtattgct tactatagtc
     7021 aatttgaatg tgtgaatcgt attcatatcg tcgacttgat aggtgctaag gcacagtatg
     7081 cccgagagtt tgattatatt aagtcattga ggagattaac aaccaaagat attgaagtag
     7141 gtggtggcat tcgtacgaag tcacaaatca tggactactt tgccgcaggg attaattatt
     7201 gcatagttgg aacgaaaggc attcaagata ctgattggct taaagagatg gcacatacat
     7261 ttccaggtcg tatttatctg tcagttgatg cttatggaga agatattaaa gtgaacggat
     7321 gggaagagga cacagagtta aatttattta gttttgtgag acagttatcg gatatacctc
     7381 ttggaggcat tatatatact gatattgcta aagatggcaa aatgtccgga cctaactttg
     7441 aattaactgg tcaattagta aaggcaacga cgattcctgt cattgcttcc ggtggtatta
     7501 gacatcagca agatattcaa cgattagcgt cgctaaatgt tcacgctgct attataggaa
     7561 aggctgcaca tcaagcatct ttttgggagg ggctagaatg attaaaaaac gtatcattcc
     7621 atgtttagat gtcaaagatg gtcgtgtcgt taaagggatt caatttaaag gattaaggga
     7681 tatagggaat cctgttgatt tagcaatata ttacaatgaa gcgggtgctg atgaattagt
     7741 atttttagac atctctaaga cggaagaggg tcatagctta atgctagaag tgattgaaca
     7801 gacagcgtca cgcttgttta tccctcttac tgtagggggt gggattcaaa gtctcgatga
     7861 tattacccaa ttgttaaatc atggtgcaga taaagtatca ttaaattcaa gtgctttaaa
     7921 aaatccacag ctcattaaac aagcgagtga taaattcggt agacaatgca tctgtatagc
     7981 aattgatagc tattatgatc ctgaaagaaa agcacattat tgttgtacgc atggtggtaa
     8041 aaaaatgaca aatattaaag tatatgactg ggtacagcaa gtagaacagt taggtgcagg
     8101 tgagctcctc gttacaagta tgggacatga tggtatgaaa caaggatttg atattgagca
     8161 tcttgcaaaa ataaaatccc ttgtaaatat tccaatcatt gcttctggtg gtggtggcaa
     8221 tgcacaacac tttgtagaac tatttaatca gacggatgtt tctgcaggtt tagctgcaag
     8281 tatattacat gaccgagaaa cgatggttca atcaattaaa gaagtgatac ggcaaggggg
     8341 tatagcagta agatgaccaa atataaaatt gattttagca aaggtttagt gccagcaatt
     8401 ttacaagata atcaaacaaa acaagtattg atgttgggtt atatgaatca agaagctttt
     8461 gataaaacga tagaagatgg tgtggtatgt ttctattcgc ggtcgaaaca acgtctatgg
     8521 acaaaaggtg aaacatctgg tcatacgcaa cttgttaaag atattcatgt agattgcgac
     8581 aatgatacta ttttaattga tgtcatacca aatggaccaa catgtcatac aggcagtcaa
     8641 agttgtttca acacagaagt tccattttca gtgcaaacat tagcgcagac agttcaagat
     8701 agtgcccaat ccaataatga aaagtcatat acaaaatatt tattaacaga aggtatagaa
     8761 aagattacaa aaaaatacgg tgaagaagct tttgaagtcg taattgaagc aattaaaggt
     8821 gacaaaaaag catttgtaag tgaagtagca gatgaacttt atcatttatt tgtcttgatg
     8881 catgcgctag gcgtcgattt ttcagaaatt gaggcggaat tagcgcgtag acatcataag
     8941 cgcaataact ttaaaggtga acgacaaaat atcgaacagt ggtaaagcaa gtatggacta
     9001 agatataagg aaaaggatca tggcttatac acttacaaat attgtggaaa acgtgacatt
     9061 ttcaagttta aaatacgaca ccaacatatt ttaactatga atgctgtgat ggtactaaag
     9121 ttgcgaactc gttatagata agtagtggat aatcacaata cgaaatcaaa aataattatg
     9181 aaaagtaaat tgagcaactc aggaatagat gtcactgtta aagatgtcga aaagtatatg
     9241 aatcgatata atgaagttat gatgggaaaa aatggcgaaa aagctaaaga gttatgtttg
     9301 tcgttactac ctattaatat catagttgtc tttacattct ttgtatttat actataaata
     9361 caaatatatc tagcctgaaa tagaaatgtc atagcctatt taaaagacaa tctccattag
     9421 aactaagatg tgcatcccga aagttagact aaaaaactaa ctttatgggg tgtattttta
     9481 tgctaatcat cataaattcg agattaagtt aaaggtagtt caagagtaat taaacaataa
     9541 attaaaaata gtaggatact tactttgagg gaagaaaatt aactgtatat atttaatgta
     9601 ggaacaagta ttacggttta tcctgataca atcattgtgg atgggatgat atttttaggt
     9661 ttaaaatacg acaccagcaa acataataac tgtaatagct cataaatctc tccatacagc
     9721 taatctaaaa aaataataca tcattggaat caagacccaa gcatgtaaat attaaaaatc
     9781 acaaaagata tatgtaaaaa agttacaa
//
LOCUS       DAHHSC010000088         9750 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.089, whole genome shotgun sequence.
ACCESSION   DAHHSC010000088 DAHHSC010000000
VERSION     DAHHSC010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 9750)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9750)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9750
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.089"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            343..807
                     /locus_tag="NBI57_001589"
     CDS             343..807
                     /locus_tag="NBI57_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCD1822529.1"
                     /translation="MDRQSFTDLIQTKFKMVRIEAGYTQDTMAQTIGLSKKTLVQIEK
                     ERVLPNWTTCISICALFRDSEVLNSTFGCDPLEIVQTISRNHCAYPNHAPTSDIYWNN
                     IETRNGYILQSNKVSNIYRVLNPDNQPIFGTSKMREAETYFNRNAKEELVHI"
     gene            986..2110
                     /locus_tag="NBI57_001590"
     CDS             986..2110
                     /locus_tag="NBI57_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF445 domain-containing protein"
                     /protein_id="HCD1822530.1"
                     /translation="MNALFIIIFMIVVGAIIGGITNVIAIRMLFHPFKPYYIFKFRVP
                     FTPGLIPKRREEIATKIGQVIEEHLLTETLINEKLKSEQSQQAIESMIQQQLQKLTKD
                     QLSIKQITSQIDIDLEQVLQTNGNQYIESQLNNYYTKHQNQTIASLLPNQLVTFLDQH
                     VDNATDLLCDRARNYLSSAKGTQDINDMLDTFFHEKGKLIGMLQMFMTKESIADRIQQ
                     ELIRLTSHPKARTIVTSLITNEYQTFKDKPLNELLDASQFNEIAENLSVYVTTYASNQ
                     ANKPVVTLMPQFVDYLEGQLSSKLANLIIEKLSIHLSTIMKKVDLRGLIEEQINTFDL
                     DYIEKLIIEIANKELKLIMSLGFILGGIIGFFQGLVAIFV"
     gene            2179..2523
                     /locus_tag="NBI57_001591"
     CDS             2179..2523
                     /locus_tag="NBI57_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002498160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF/YmcA family competence regulator"
                     /protein_id="HCD1822531.1"
                     /translation="MAVNLYDYANQLEQALRESEEYKAIKEAFANVKANEESKKLFDE
                     FRETQINFQQKQMQGEEIAEEDLQKAQEQAQAIEKDENISALMNAEQKMSQVFQEINQ
                     IIVKPLDEIYAD"
     gene            3062..3160
                     /locus_tag="NBI57_001592"
     CDS             3062..3160
                     /locus_tag="NBI57_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1822532.1"
                     /translation="MSVGSLAVLTITEIAFPTEIFEKNQRTLQIKF"
     gene            3451..4647
                     /locus_tag="NBI57_001593"
     CDS             3451..4647
                     /locus_tag="NBI57_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HCD1822533.1"
                     /translation="MVKFIHCSDLHLDSPFKSKSHISPKIFEDVQKSAYESFKNIVDI
                     ALQQDVDFVIIAGDLFDSENRTLRAEIFLKQQFERLQNEQIFVYVCHGNHDPLSSKIS
                     SNWPDNVSVFSNKVETYEAITKSGETIYIHGFSYENRASYENKIDEYPSSQGQKGIHI
                     GVLHGTYSKSSVNERYTEFILEDLNSKLYHYWALGHIHERQQLSDMPVINYSGNIQGR
                     HFNEQGEKGCLLIEGDHLKLKTKFYPTQYIRFEEATIETDKTSKQGLYEVIQNFKEQV
                     REEGKAFYRLTLVINSETLISPQDLLQVEEMITDYEENENQFVYIDELKIQYAQNDES
                     PLVNEFSAELLVDQTVFDKAMSDLYLNPRASKFLDDYGTFDHTALVNRAEEILKAEMR
                     GEQNDN"
     gene            4637..7570
                     /locus_tag="NBI57_001594"
     CDS             4637..7570
                     /locus_tag="NBI57_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCD1822534.1"
                     /translation="MIIKSLEIYGYGQFVQRKIEFNKNFTEIFGENEAGKSTIQAFIH
                     SILFGFPTKKSKEPRLEPRLGNQYGGKLVLILDDGLEIEVERIKGSAQGDVKVYLPNG
                     AVRDDAWLQKKLNYISKKTYQGIFSFDVLGLQDIHRNLNEKQLQDYLLQAGALGSTEF
                     TSMREVINRKKDELYKKSGKNPIINQQIEQLKQLESQIREEAKLETYHRLVDDRDKSS
                     RRLENLKHNLNQLSKMHEEKQKEVALHDHSQEWKSLEQQLNIEPITFPEKGVDRYEKA
                     RAHKQSLERDIGLRNERLAQLKEEATQLEPVKQSDIDAFISLNQQENEIKNKEFELTA
                     IEKDIANKQRDKDELQANIGWSETHHDVDSSEAMKSYVSEQIKNKQEQAAYIKQLERS
                     LEENKIEDNAVHSELDSVEEKIVPEETFEKKKEYSQQVIELNEKENLYSKLKERFEIE
                     QQEKQKRQKLLRTTFILLTLVGIGLTAFSFISNNMLFGIIFAVLTLVFVIGIIMSKSK
                     EVDYSEAITDEIEEIKAQLAILDENYDLDFDLDEQYRIRDHWQQALKNKDILEEKRQY
                     IEGRLNDAKGRHDELQSTVENVKDELYLSSKISNDLIVDSISTMANIKALDQHISDLN
                     QQRQQLVQELDTFYNHAEAVTKSQFVYFNKLSLFHDVQQWLKSAEDTNEKWRINAENT
                     KLVTNELNHLNAQLEENNKEITALFDFINVGTEEDFYQHHEDYQTYTSNLSRFNDLTK
                     YLENQNYSYELSSSLSEKTTAQLEEEDHLLATQVDEYNEQYLEMQAQVSDLSAQINHM
                     ETDTTLANLRHEYHSLKNQLNDIAKDWASLSYLQSLVDEHIKQIKDKRLPQVINEAVE
                     ILKHLTDGRYTMINYNEDSITVKHVNGQLYDPVELSQSTKELLYVALRISLIKVLRPY
                     YPFPLIVDDAFVHFDKKRTEKMLNYLRSLSEHYQVLYFTCVKDNIVPSKEVITLNKIE
                     EGGKR"
     gene            7567..8508
                     /gene="yhaM"
                     /locus_tag="NBI57_001595"
     CDS             7567..8508
                     /gene="yhaM"
                     /locus_tag="NBI57_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exoribonuclease YhaM"
                     /protein_id="HCD1822535.1"
                     /translation="MRNIENLNPGDSVDHFFLVHKATQGVTAQGKDYMTLHLQDKSGE
                     IEAKFWTATKNDMATIKPEEIVHVKGDIINYRGNKQMKVNQIRLATTEDQLKTEQFVD
                     GAPLSPAEIQEEISHYLLDIENANLQRITRHLLKKYQERFYTYPAASSHHHNFASGLS
                     YHVLTMLRIAKSICDIYPLLNKSLLYSGIILHDIGKVRELSGPVATSYTVEGNLLGHI
                     SIASDEVVEAARELNIEGEEIMLLRHMILSHHGKLEYGSPKLPYLKEAEILCYIDNID
                     ARMNMFEKAYKKTDKGQFTDKIFGLENRRFYNPESLD"
     gene            complement(8629..9591)
                     /locus_tag="NBI57_001596"
     CDS             complement(8629..9591)
                     /locus_tag="NBI57_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="foldase"
                     /protein_id="HCD1822536.1"
                     /translation="MKMINKLIVPVTASALLLGACGASATDSKENTLISSKAGDVTVA
                     DTMKKIGKDQIANASFTEMLNKILADKYKNKVNDKKIDEQIEKMQKQYGGKDKFEKAL
                     QQQGLTADKYKENLRTAAYHKELLSDKIKISDSEIKEDSKKASHILIKVKSKKSDKEG
                     LDDKEAKQKAEEIQKEVSKDPSKFGEIAKKESMDTGSAKKDGELGYVLKGQTDKDFEK
                     ALFKLKDGEVSEVVKSSFGYHIIKADKPTDFNSEKQSLKEKLVDQKVQKNPKLLTDAY
                     KDLLKEYDVDFKDRDIKSVVEDKILNPEKLKQGGAQGGQSGMSQ"
BASE COUNT         3607 a         1378 c         1658 g         3107 t
ORIGIN      
        1 atatttgtcg aaaaatagac aatttttaat ctgattttga tgaatttttt atagcgatag
       61 ggtttattat acagttttct ttgtaaattt gatatcatta taaaagtgta agttttatca
      121 aatctagtga ttgtgcataa aattttactt catttacttt cgcttcatta taaagtaaac
      181 ttaactcttg ttgacttgat caaaatttaa tacaaatgtg aaattgactc ttttcatttt
      241 attaaaaata tattaagatg taatctgtgt ttcaagaaat gtgtattatt gcaatttctt
      301 gtgtccggat aaaattcccc aaacagaaag gtagataaca gaatggatag acagagtttt
      361 acagatttaa ttcaaacaaa atttaaaatg gttcgtatag aggctggtta tacgcaagat
      421 actatggcgc aaacaattgg actttctaaa aagactttag tacaaatcga aaaagagaga
      481 gtattaccaa actggacaac ttgtatttca atttgtgcat tattcagaga ctctgaagta
      541 ttaaacagta catttggctg tgatccatta gaaatcgttc aaacaatttc aagaaatcac
      601 tgtgcatatc caaaccatgc accaacaagt gatatttatt ggaataacat tgagactcgt
      661 aatgggtaca ttttacaaag taacaaagta agcaatattt atcgtgtatt aaatccagat
      721 aaccaaccaa ttttcggtac ttctaaaatg agagaagctg aaacatactt taacagaaat
      781 gctaaagaag aattagtaca tatctaggcc tatatacttt taataaataa taaaagcgat
      841 gtaacacacc tgtttcgaga ggtgaggcat cgctttttgt atgtaaacta ataataaatt
      901 gagcagaaag tgaaatgatt aatgaattaa aaaataaaaa tagtggtata atgaataact
      961 atgttgtcac aaaggagtca aaattatgaa tgcactattt attatcatat ttatgattgt
     1021 agtaggggcg ataattgggg gcattactaa tgtaattgca attagaatgc tgtttcaccc
     1081 ctttaaacca tactatatat ttaaatttag agtcccattt acaccaggtt taataccgaa
     1141 aagacgcgaa gaaattgcaa ctaaaattgg ccaagtgatt gaagagcatt tgcttacaga
     1201 aactttaatt aatgaaaaat taaaaagcga gcaatcacag caagcaatag aatctatgat
     1261 tcaacagcag ttacaaaagt tgacgaaaga tcaattgtca ataaaacaaa ttacttctca
     1321 aatcgatatt gatttagaac aagtattaca aactaatgga aatcaatata ttgaatcaca
     1381 attgaataat tattatacaa agcatcaaaa ccaaacaata gcatctttat tgccaaatca
     1441 acttgtaaca tttttagatc agcatgtaga taacgcaaca gacttattat gtgatcgtgc
     1501 aaggaattat ttatcatctg caaaaggcac acaagatatt aatgatatgt tggatacttt
     1561 tttccatgag aaaggtaagt taattggtat gttgcaaatg tttatgacaa aagagagcat
     1621 tgcagatcgc attcaacaag aacttatacg tttaacatct catcctaaag caagaacaat
     1681 tgtgacatcg ttaattacca atgaatatca aacttttaaa gataaaccat tgaatgaact
     1741 gttagatgca tcgcagttta atgaaatcgc tgaaaatcta tcagtgtatg taacaacata
     1801 tgcaagcaat caagcgaata agccagtggt tacgttaatg cctcaattcg ttgattattt
     1861 agaaggtcaa ctctctagca aattagcaaa tttaatcatt gaaaaattat cgattcactt
     1921 atctacaatt atgaaaaaag tggatttacg aggattaatt gaagaacaaa ttaatacgtt
     1981 tgacttagat tatattgaaa aattaattat agaaattgct aacaaagaac taaagttgat
     2041 tatgtcatta ggtttcattt taggtggtat tattggattt ttccaaggtt tggttgcaat
     2101 ctttgtctaa aaaagcgaga tgtggtaaag taatatcgaa cgttttaata aacatcacaa
     2161 caaaggagtg ctataaccat ggcagtaaat ttatatgatt atgcaaatca attagaacaa
     2221 gctttaagag aaagcgaaga atacaaagca atcaaagaag cattcgctaa tgtaaaagct
     2281 aacgaagaat ctaaaaagtt attcgatgag ttccgtgaaa ctcaaattaa cttccaacaa
     2341 aaacaaatgc aaggtgaaga aattgctgaa gaagatttac aaaaagcgca agaacaagcg
     2401 caagcaattg aaaaagatga aaacatctct gcattaatga atgctgaaca aaaaatgagt
     2461 caagtattcc aagaaatcaa ccaaattatc gttaaaccat tagacgaaat ttacgctgac
     2521 taatattaaa ataatatttt tcaatccttt aaatagagga atagactgcc gacagagtcc
     2581 gagactttgt gggtagtttt tttagttttg ataacggaag ttagaggctc tctgtcaaat
     2641 tgggcagatg cgttcactat cggaatttag gctactaaac atgtcctagc tttagctcag
     2701 aatggacgac ggaagctggg caatcaaaca tggctagctc tatgccaaaa cgggcagatg
     2761 agcttgataa cggaagttag aggttctatg tcaaattggg cagatgcatt cagtaacgga
     2821 agttaggcta gtaaacgtgt cctaacttcc tttttgcatt ttgaagtgta aagttttcaa
     2881 cataatacta ttagttcggt catgtatcgg actgatggaa aagcgtttca cttttaatga
     2941 ctcattaaga acggcctgaa aatgtttggc gtattaagtg caatgatagt tttgacattt
     3001 agtttctaat tggtcattac tgccgagcaa atctagtaga gtaatcatgt aaatctttaa
     3061 tgtgtcagtt ggttcactag cggtgttaac aattacggaa attgcatttc cgactgaaat
     3121 ttttgaaaaa aatcaacgca cgctacaaat aaaattttaa actgttataa atgtgtctca
     3181 atttcatatg ttcatcgacg atatgaagcg tattatggta aaatgaagaa ataataaact
     3241 tgttaataaa taaaacatca cgatttgact aaagcacttt attattgtgt agataataat
     3301 tttttaacga aataaaaatg gcgactggtt ttaataaatc agctaatgaa tcactacacc
     3361 tataagtatg aatatagtga ttagaatgct ttgtatagtt ggattttgca aaattgatgt
     3421 tagaaccttt aattaaggag atatggatgt atggttaaat ttattcattg ttcggattta
     3481 catttagata gtcctttcaa atctaagagt catataagcc ctaaaatttt tgaggatgtt
     3541 caaaaaagtg cttatgaaag ttttaaaaat attgtagata ttgcattaca acaagatgtt
     3601 gattttgtaa ttatcgcggg tgatttattt gatagtgaaa acagaacatt acgtgctgaa
     3661 attttcttaa aacagcaatt tgaacgttta caaaatgaac aaatctttgt ttatgtttgt
     3721 catggtaatc acgatccttt atcatcaaag atttcatcaa actggccaga taatgtttct
     3781 gtattttcaa ataaagttga gacgtatgaa gcaattacta aatctggtga aacaatttat
     3841 attcacggat ttagttatga aaatagagca agttatgaga acaagattga tgaatatcca
     3901 tcaagtcaag gccaaaaagg catacatatt ggtgtcttgc atggtacgta tagtaaatct
     3961 tcagttaacg aaagatatac cgagttcatt ttagaagatt taaacagtaa attgtatcat
     4021 tattgggctt taggtcatat acatgaacgt caacaattaa gtgatatgcc tgtaattaac
     4081 tattcaggta atattcaagg tagacatttt aatgagcaag gtgaaaaagg ttgcttatta
     4141 atcgagggtg accacttaaa attaaagact aaattttatc ctacacagta tattagattt
     4201 gaagaagcaa ctattgaaac ggataagaca tctaagcaag gtttatacga ggtcattcaa
     4261 aactttaaag aacaagtgag agaagaagga aaagcctttt atcgtttaac gcttgttatt
     4321 aatagtgaga cattaatttc acctcaagat ttattacaag ttgaagaaat gattacagat
     4381 tatgaagaaa acgaaaatca atttgtatat attgatgagt taaaaataca atatgcacaa
     4441 aatgatgagt cacctttagt taatgaattt tcagcggaat tattagtcga tcaaactgtt
     4501 tttgataaag cgatgtcaga tttatattta aatccaaggg catctaagtt cctagacgat
     4561 tatggaacat tcgaccatac agcattagtt aatcgtgctg aagaaatatt aaaagctgaa
     4621 atgagaggtg aacaaaatga taattaaatc acttgaaatt tatggttacg gtcaatttgt
     4681 tcaacgtaaa attgaattta ataaaaactt cactgaaatt tttggtgaaa atgaagcggg
     4741 taaatcgacg attcaagcat tcatccattc gatattattt ggttttccaa ctaaaaagtc
     4801 taaagagcca agactagaac cacgtctagg taaccaatac ggtggtaaat tagtacttat
     4861 tcttgatgat ggcttagaga ttgaagttga acgaattaaa ggcagtgctc aaggtgatgt
     4921 gaaagtatat ttacctaatg gtgctgtgcg tgatgatgct tggttacaaa agaaacttaa
     4981 ttatatttct aaaaagacat atcaaggtat cttttcattt gatgtactag ggcttcaaga
     5041 cattcataga aatctaaatg aaaaacaatt gcaagattat ttattacaag caggggcttt
     5101 aggatcaact gaattcacgt caatgcgcga agtgattaat cgtaaaaaag atgaattata
     5161 taaaaaatca ggtaaaaatc cgatcattaa tcaacaaatt gagcaattaa aacaactaga
     5221 aagtcaaatt cgtgaagaag caaagctaga aacatatcat cgcttagtag atgatcgaga
     5281 taaatcatca cgtcgattag agaatttaaa gcataattta aatcaattat caaaaatgca
     5341 tgaagaaaaa caaaaagagg ttgctttaca tgatcattca caagaatgga agtctctaga
     5401 acaacagtta aatattgagc caatcacatt cccagaaaaa ggtgtggatc gttacgaaaa
     5461 agcgcgagcg cataagcaat cgttagaaag agatattggt ttaagaaatg agcgtttagc
     5521 tcaacttaaa gaagaagcga ctcaattaga gccagttaaa caatctgata ttgacgcctt
     5581 cattagtttg aatcaacaag aaaatgaaat taaaaataaa gaatttgaac ttactgcaat
     5641 cgaaaaggat attgcgaata aacaacgtga taaagatgaa ttgcaagcaa atattggttg
     5701 gtctgaaacg catcatgacg tagatagttc agaggcaatg aaaagttatg tcagtgagca
     5761 aatcaagaat aaacaagaac aagctgcata cattaaacaa ttagaacgta gtttagaaga
     5821 aaataaaatc gaagataatg cggttcatag cgaactagat tctgttgaag aaaaaatagt
     5881 tcctgaagag acttttgaaa agaaaaaaga atactcacaa caagtcattg aattaaacga
     5941 aaaagagaac ttgtatagca aattgaaaga acgtttcgaa attgagcaac aagaaaaaca
     6001 aaaacgtcaa aaattgttac gaacaacgtt tattctttta acattagtag gtattggttt
     6061 aactgccttc tcatttattt caaacaacat gttgtttggt attattttcg cagtattaac
     6121 tttagtattt gttatcggta tcatcatgtc taaatcaaaa gaagtagatt atagcgaggc
     6181 catcacagat gaaatcgaag aaattaaagc acaactagcc attttagatg aaaattatga
     6241 cttagatttt gatcttgatg agcaatatcg cattcgtgat cactggcaac aagctttgaa
     6301 aaataaagat attttagaag agaaacgtca gtatattgaa ggtcgtttaa atgatgcgaa
     6361 agggcgtcat gatgaattac aatcgacagt tgagaacgtt aaggatgaat tatacttatc
     6421 atctaaaatt tcaaatgatt taattgttga tagtatttct acaatggcga atattaaagc
     6481 attagatcaa catatcagtg atttaaatca acaacgccag caattggttc aagaattaga
     6541 tacattctac aatcatgctg aagctgttac aaaatcacaa tttgtttatt tcaacaaatt
     6601 atctttattc cacgatgtgc aacaatggtt gaaatcagct gaagacacga atgaaaaatg
     6661 gcgtattaat gctgaaaata ccaaacttgt tacaaatgaa ttaaatcatt taaatgctca
     6721 attagaagaa aataataaag aaattacagc actattcgat tttatcaatg ttggtactga
     6781 agaagatttt taccaacatc atgaagatta ccaaacttat actagtaatt tgagtcgttt
     6841 taatgattta actaaatatc ttgagaacca aaactattct tatgaattaa gttctagttt
     6901 aagtgaaaaa acaactgcac aacttgaaga agaagatcat ttattggcta ctcaagttga
     6961 cgaatataat gagcaatatc ttgaaatgca agcacaagtc agtgatttaa gtgcacaaat
     7021 caatcacatg gaaactgata caacgcttgc taatttaaga catgaatatc acagtcttaa
     7081 aaatcaactt aatgatatcg caaaagattg ggcaagttta agttatttac aaagtttagt
     7141 tgatgaacac attaaacaaa ttaaagataa acgtttgcct caagttatta atgaagcggt
     7201 agaaatattg aagcatttaa cagatggcag atatacgatg attaactata atgaagattc
     7261 aattacggtt aaacatgtta atggtcaatt atatgatcct gttgaactaa gtcaatctac
     7321 aaaagaatta ctttatgtag ctttacgtat cagtttaatt aaggtactaa gaccatatta
     7381 tccgttccca ttaattgttg atgatgcatt tgttcatttt gataaaaagc gtactgaaaa
     7441 aatgttgaat tatttacgat cattatcaga acactatcaa gtactatact ttacatgtgt
     7501 aaaagataat attgttccat caaaagaagt gattacatta aacaaaatag aggaaggcgg
     7561 gaaacgatga gaaatataga gaatctaaat cccggagatt cagttgatca ctttttctta
     7621 gtgcataaag ctacacaggg tgtaacagca caaggtaaag attatatgac attacatttg
     7681 caagataaaa gtggtgaaat tgaagcgaaa ttttggacgg ctacaaaaaa tgatatggca
     7741 acaatcaagc ctgaagaaat tgtacatgtt aaaggtgaca tcataaacta tcgcggaaat
     7801 aaacagatga aagtcaacca aattagacta gcgacaactg aagatcaatt aaaaacagaa
     7861 caatttgtag atggtgcacc tttatcaccg gcagaaatac aagaagagat ttctcattat
     7921 ttgctagata ttgaaaatgc taatttacaa cgtatcacac gtcatttatt gaaaaaatat
     7981 caagaacgat tttacacata tccagctgct agttctcatc atcataactt tgcgagtggc
     8041 ttaagctatc atgtattgac gatgttacgt attgcaaaat caatttgtga catttatcca
     8101 ttgttaaaca aaagtttgtt atatagtggt attattttgc atgatattgg taaagttaga
     8161 gaattgagtg gtcctgttgc gacgtcgtat acagtcgaag gtaacttatt aggacacatc
     8221 tcgattgcga gtgatgaagt agttgaagca gctcgtgaat tgaacattga aggagaagaa
     8281 atcatgttgt taagacatat gattttatct catcatggta agttagagta tggttcgcca
     8341 aaactgccat acttaaaaga agcagaaatt ttatgctata tcgataacat cgatgctaga
     8401 atgaatatgt ttgaaaaggc ttataaaaaa actgacaagg gtcagtttac agataaaata
     8461 tttggtcttg aaaatcgtag attctacaat cctgaatcac tcgattaaga attaaaagat
     8521 atcggacaga tgagtatact gaaacatgag ctcctctgtc cgatttttaa tgcgaaaaaa
     8581 ccgagcggcc gtggtatgat ttttgaacca cggtcgctcg gttttgtgtt attggctcat
     8641 gccggattgt ccgccttgtg cgccaccttg tttaagtttt tcagggttta agattttatc
     8701 ttcgacaact gatttaatat cacgatcttt aaagtcaaca tcgtattctt ttaatagatc
     8761 tttgtatgca tcagttaata attttggatt tttttgtact ttctgatcga ctaatttttc
     8821 tttcaggctt tgtttttcac tgttaaagtc tgttggttta tcagctttaa taatatgata
     8881 tccaaagctt gatttaacaa cctctgatac ttcaccatct ttaagcttaa atagtgcttt
     8941 ttcaaaatct ttatcagttt gtcctttaag aacataacct aattcgccat cttttttagc
     9001 tgaaccagta tccattgatt cttttttagc gatttcacca aatttacttg gatcttttga
     9061 aacttctttt tgaatttctt cagctttttg tttcgcttct ttatcgtcta agccttcttt
     9121 gtcgcttttc ttagatttaa ctttaattaa aatgtgtgaa gctttcttgc tgtcttcttt
     9181 aatttcagaa tcagagattt taattttatc tgatagtaat tctttatgat aagcagcagt
     9241 acgtaaattt tctttatatt tatcggctgt taaaccttgc tgttgaaggg ccttttcaaa
     9301 tttatcttta ccgccgtatt gcttttgcat tttttcaatt tgttcgtcaa tcttcttatc
     9361 attaacttta tttttatatt tatcagctaa aattttattt aacatttcag taaatgatgc
     9421 atttgcaatt tgatctttac cgattttttt cattgtatct gcaactgtta catctccagc
     9481 tttagaagaa attaatgtat tttctttaga gtctgtggca ctagcgccac aagcgcctaa
     9541 taataaagca ctagctgtta ccggaacgat taatttgttt atcatcttca tagttgaaac
     9601 tcctttgtaa gttaatcatc gtgaatataa catagtttta tattatcacc aatcaataac
     9661 ccatgacatc agactagagc attaggttta ggggtagaaa ttgatgaaaa taaaaaaaca
     9721 aggtctatat acatttgatt gtaagctatt
//
LOCUS       DAHHSC010000089         9561 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.090, whole genome shotgun sequence.
ACCESSION   DAHHSC010000089 DAHHSC010000000
VERSION     DAHHSC010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 9561)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9561)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9561
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.090"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            <1..267
                     /locus_tag="NBI57_001597"
     CDS             <1..267
                     /locus_tag="NBI57_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-binding protein"
                     /protein_id="HCD1822537.1"
                     /translation="IIPKTPNNDNVIPYVDEAIKIIDESGLHFRVGPLETTVQGNMNE
                     CLILIQSLNERMVELECPSIISQVKFYHVPDGITIETLTEKYDE"
     gene            401..1945
                     /gene="miaB"
                     /locus_tag="NBI57_001598"
     CDS             401..1945
                     /gene="miaB"
                     /locus_tag="NBI57_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HCD1822538.1"
                     /translation="MNEEQRKASSVDVLAERDKKAEKDYSKYFEHVYQPPNLKEAKKR
                     GKQEVRYNRDFQIDEKYRGMGNERTFLIKTYGCQMNAHDTEVIAGILEALGYQATTDI
                     NTADVILINTCAIRENAENKVFSEIGNLKHLKKERPDILIGVCGCMSQEESVVNKILK
                     SYQNVDMIFGTHNIHHLPEILEEAYLSKAMVVEVWSKEGDVIENLPKVREGNIKAWVN
                     IMYGCDKFCTYCIVPFTRGKERSRRPEDIIDEVRELAREGYKEITLLGQNVNSYGKDL
                     QDIEYDLGDLLQAISKIAIPRVRFTTSHPWDFTDHMIDVISEGGNIVPHIHLPVQSGN
                     NAVLKIMGRKYTRESYLDLVKRIKDRIPNVALTTDIIVGYPNESEEQFEETLTLYDEV
                     GFEHAYTYLYSQRDGTPAAKMKDNVPLNVKKERLQRLNKKVGHYSQIAMSKYEGQTVT
                     VLCEGSSKKDDQVLAGYTDKNKLVNFKAPKEMIGKLVEVRIDEAKQYSLNGSFVKEVE
                     PEMVIQ"
     gene            1946..2311
                     /locus_tag="NBI57_001599"
     CDS             1946..2311
                     /locus_tag="NBI57_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RicAFT regulatory complex protein RicA family
                     protein"
                     /protein_id="HCD1822539.1"
                     /translation="MYNKDDVLKQADNIANKIKNLDTIKTYQQIEAQIHQNQTIKTKM
                     DMLKKHQKQAVNFQNYGKQNALEQSEHTIQSIEAEINTLPIVEQFQTSQYEANQLLKM
                     FVSTMETRLNDHNKAKHSD"
     gene            2338..2829
                     /gene="thiW"
                     /locus_tag="NBI57_001600"
     CDS             2338..2829
                     /gene="thiW"
                     /locus_tag="NBI57_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy coupling factor transporter S component
                     ThiW"
                     /protein_id="HCD1822540.1"
                     /translation="MKSRKLAITALLIAINVVLSSIIIIPLGPVKAAPVQHFVNVLSA
                     VIVGPWYGLAQALISSILRVLFGTGTAFAFPGSMIGVLLASMFYIYRKHIFMAAVGEV
                     LGTGVIGSLICIPLAYFLGLQDFFIKPLMITFIVSSAIGSIISYFLLITLKKRGILQR
                     FIK"
     gene            3132..5750
                     /gene="mutS"
                     /locus_tag="NBI57_001601"
     CDS             3132..5750
                     /gene="mutS"
                     /locus_tag="NBI57_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011275903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HCD1822541.1"
                     /translation="MSNVTPMMQQYLKIKSEYQDCLLFFRLGDFYEMFYEDAKEASRV
                     LEITLTKRDAKKENPIPMCGVPYHSADSYIDTLVNNGYKVAICEQMEDPKQTKGMVRR
                     EVVRIVTPGTVMEQGGVDDKQNNYILSFVMNQPEIALSYCDVSTGELKVTHFNDEATL
                     LNEITTINPNEVVINDNISDNLKRQINMVTETITVRETLSSEIYSVNQTEHKLMYQAT
                     QLLLDYIHHTQKRDLSHIEDVVQYAAIDYMKMDFYAKRNLELTESIRLKSKKGTLLWL
                     MDETKTPMGARRLKQWIDRPLISKEQIEARLDIVDEFSAHFIERDTLRTYLNQVYDIE
                     RLVGRVSYGNVNARDLIQLKHSISEIPNIKALLNSMNQNTLVQVNQLEPLDDLLDILE
                     QSLVEEPPISVKDGGLFKVGFNTQLDEYLEASKNGKTWLAELQAKERQRTGIKSLKIS
                     FNKVFGYFIEITRANLQNFEPSEFGYMRKQTLSNAERFITDELKEKEDIILGAEDKAI
                     ELEYQLFVQLREEVKKYTERLQQQAKIISELDCLQSFAEIAQKYNYTRPSFSENKTLE
                     LVESRHPVVERVMDYNDYVPNNCRLDNETFIYLITGPNMSGKSTYMRQVAIISIMAQM
                     GAYVPCKEAVLPIFDQIFTRIGAADDLVSGKSTFMVEMLEAQKALTYATEDSLIIFDE
                     IGRGTSTYDGLALAQAMIEYVAETSHAKTLFSTHYHELTTLDQALPSLKNVHVAANEY
                     KGELIFLHKVKDGAVDDSYGIQVAKLADLPEKVISRAQVILSEFEASAGKKSSISNLK
                     MVENEPEINQENLNLSVEETTDTLSQKDFEQASFDLFENDQESEIELQIKNLNLSNMT
                     PIEALVKLSELQNQLK"
     gene            5763..7772
                     /gene="mutL"
                     /locus_tag="NBI57_001602"
     CDS             5763..7772
                     /gene="mutL"
                     /locus_tag="NBI57_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HCD1822542.1"
                     /translation="MGKIKELQTSLANKIAAGEVVERPSSVVKELLENAIDAGATEIS
                     IEVEESGVQSIRVVDNGSGIEAEDLGLVFHRHATSKLDQDEDLFHIRTLGFRGEALAS
                     ISSVAKVTLKTCTDNANGNEIYVENGEILNHKPAKAKKGTDILVESLFYNTPARLKYI
                     KSLYTELGKITDIVNRMAMSHPDIRIALISDGKTMLSTNGSGRTNEVMAEIYGMKVAR
                     DLVHISGDTSDYHIEGFVAKPEHSRSNKHYISIFINGRYIKNFMLNKAILEGYHTLLT
                     IGRFPICYINIEMDPILVDVNVHPTKLEVRLSKEEQLYQLIVSKIQEAFKDRILIPKN
                     NLDYVPKKNKVLYSFEQQKIEFEQRQNTENNQEKTFSSEESNSKSFMAENQNDEIVIK
                     EDSYNPFVTKTSESLITDDESSGYNNTREKDEDYFKKQQEILQEMDQTFDSNEDASVQ
                     NYENKASDDYYDVNDIKGTKSKDPKRRIPYMEIVGQVHGTYIIAQNEFGMYMIDQHAA
                     QERIKYEYFRDKIGEVTNEVQDLLIPLTFHFSKDEQLVIDQYKNELQQVGIMLEHFGG
                     HDYIVSSYPVWFPKDEVEEIIKDMIELILEEKKVDIKKLREDVAIMMSCKKSIKANHY
                     LQKHEMSDLIDQLREAEDPFTCPHGRPIIINFSKYELEKLFKRVM"
     gene            7778..8320
                     /locus_tag="NBI57_001603"
     CDS             7778..8320
                     /locus_tag="NBI57_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate responsive antiterminator"
                     /protein_id="HCD1822543.1"
                     /translation="MNQVNNNILPAIRNIKDLEKLIKTDYKMCVLLDMHIGHIKSIME
                     LLKQNHIECFIHIDLIKGLSHDEFASEFIIQQYKPKGIVSTKSKVIKKAKSLNTLTIF
                     RVFIIDSQALKRSIDLIKKVEPDFVEVLPGVASKAIHHIQKETNTQVIAGGLINTIDE
                     VNEAVKNGAKYVTTSYDKLW"
     gene            8795..>9561
                     /locus_tag="NBI57_001604"
     CDS             8795..>9561
                     /locus_tag="NBI57_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HCD1822544.1"
                     /translation="MNVYLAEFLGTAILILFGGGVCANVNLKRSAANGADWIVITAGW
                     GLAVTMGVFAVGQFSGAHLNPAVSLALALDGSFDWSLVPGYIVAQMLGAIVGATIVWL
                     MYLPHWKATEEAGAKLGVFSTAPAIKNYFANFLSEIIGTMALTLGILFIGVNKIADGL
                     NPLIVGALIVAIGLSLGGATGYAINPARDLGPRIAHAILPIAGKGGSNWSYAIVPILG
                     PIAGGLLGAVVYAVFYKHTFNIGCAIAIVVVIITLILG"
BASE COUNT         3629 a         1248 c         1793 g         2891 t
ORIGIN      
        1 ataattccta aaacaccaaa caatgacaat gttatacctt acgtagacga ggcgattaaa
       61 ataattgacg aatctggttt gcattttaga gtaggtccgt tagaaacgac agtacaagga
      121 aatatgaatg aatgtttaat tttaatacaa tcattaaatg aacgaatggt ggaacttgaa
      181 tgtccaagta ttattagcca agttaagttt tatcatgtgc cagatggcat cactattgaa
      241 actttaactg aaaaatatga tgaataacat taaaagtgaa gtaaactgga tttgaattgg
      301 cttgttagag atgacgtata actttaactg tttttgcact ttatagttaa atttaatata
      361 attattaaat gatacgggca aatagaaagg attttgtaaa gtgaacgaag aacaaagaaa
      421 agcaagttct gtagatgttt tagctgagag agataagaaa gcagaaaaag attatagtaa
      481 atattttgaa catgtttatc agccgcctaa tttaaaagaa gcgaaaaaaa gaggtaaaca
      541 agaagttcgt tataatagag atttccaaat tgatgaaaaa tatcgcggta tggggaacga
      601 gcgtacattt ttaattaaaa catatggatg tcaaatgaat gcacatgaca ctgaggtcat
      661 tgctggtata cttgaagcat taggctatca agcaacgact gatattaaca ctgcagatgt
      721 tattttaatt aatacatgtg cgattagaga aaatgccgag aacaaagtgt ttagtgaaat
      781 aggtaatttg aagcatttga aaaaagaacg acctgatatt ttaatcggtg tttgtggttg
      841 tatgtcacaa gaagagtcag tagtgaataa aattttaaaa tcgtatcaaa atgtagatat
      901 gatatttggt acacataata ttcatcattt accagaaatt ttagaagaag catacttatc
      961 taaagcaatg gttgttgaag tatggtctaa agaaggagac gttattgaaa atcttccaaa
     1021 agtccgtgaa ggcaacatta aagcatgggt caatattatg tatggttgtg ataagttttg
     1081 tacatattgt attgttccat ttacaagagg taaagaacga agccgtagac ctgaagacat
     1141 tatagatgaa gtacgtgaac ttgctcgtga aggttacaaa gaaataacgc ttttaggtca
     1201 aaatgtaaat tcttatggta aagatttaca ggatatagaa tatgacttag gagatctttt
     1261 acaagcaatt tctaaaatag cgattccaag agttcgtttc acaacaagtc atccttggga
     1321 ctttacagat cacatgattg atgttatttc agagggtggt aatatcgttc ctcatatcca
     1381 cttgccagtt caatctggaa ataatgcagt attaaaaata atgggtagaa aatatacacg
     1441 agaaagttat ttggatttag taaaacgaat caaagataga attcctaatg tagcattaac
     1501 tacagatatt attgtagggt atccaaatga atcagaggaa caatttgaag aaactttaac
     1561 tctgtatgat gaagttggtt ttgaacatgc atatacgtac ttgtattcac aacgtgatgg
     1621 tacgcctgct gctaaaatga aagataatgt acctttaaat gtcaaaaagg aacgattgca
     1681 acgtttgaat aaaaaagttg gtcattattc acaaatagct atgagtaagt acgaaggaca
     1741 aactgtaaca gtactttgtg aaggtagtag taaaaaagat gatcaggttc ttgctggcta
     1801 cactgataaa aataagctag ttaatttcaa agcacctaaa gaaatgattg gtaaactagt
     1861 ggaagtacga atagatgaag ctaaacagta ttcattaaat ggcagttttg taaaggaagt
     1921 agagccggaa atggtgattc aataaatgta taataaagat gacgtgttga aacaagcgga
     1981 taatattgca aataaaatta aaaatttgga tactatcaaa acatatcaac aaattgaagc
     2041 acagattcat cagaaccaaa cgataaagac taaaatggat atgttaaaaa agcatcaaaa
     2101 acaagcagta aactttcaaa attacgggaa acaaaatgcg ctagaacagt cggaacatac
     2161 cattcaaagt atagaagcag aaataaatac attgcccata gttgaacagt ttcaaacttc
     2221 acaatatgaa gcgaatcaat tattgaaaat gtttgtatca acaatggaaa cacgtttaaa
     2281 tgaccataat aaagccaagc atagtgatta attacaaaca aaaaggagaa caatcatatg
     2341 aaatcaagaa aactggctat aactgcactt ttaattgcaa taaatgttgt attaagcagt
     2401 attatcatca ttcctctagg accagttaag gcagcaccag tacagcattt tgtaaatgta
     2461 ttaagtgcgg tcatagtagg tccttggtat ggattagctc aagcgcttat atcatcaatt
     2521 ttaagagttc tttttggtac tggtacagct tttgcatttc caggtagtat gattggagtt
     2581 ttattggcta gtatgtttta catatatcgt aaacatatat tcatggccgc ggtcggtgaa
     2641 gtacttggaa ctggtgtcat cggaagttta atttgtatac cattagcata tttccttggg
     2701 cttcaagact tcttcattaa accgctaatg attacgttca tagtctcaag tgctatcgga
     2761 tctattataa gttatttctt attaattact ctaaaaaaac gtggtattct tcaaaggttt
     2821 ataaaataat tttatgatat atgatttcaa acagagtcat tgttcatttt aatattgaac
     2881 gcccttactt aattaaatgt cttgaatgtt agaattttaa tttaattcta aacatgctat
     2941 tgacacattt tattaggttt gaggcgtttt cttatacatt taagatgctt cgtgattata
     3001 tggtatgaat aggtttgtaa aagtaagtat gttaaaatgt ataagttaaa taattaataa
     3061 ttagaaagct tacaaacgaa tatattgtaa agacaaattt taaaaagaga caagggttgt
     3121 gaagaaataa tatgtctaat gttacaccaa tgatgcagca atatttaaaa ataaaatcag
     3181 aataccaaga ttgcttatta ttttttagac taggtgattt ctatgaaatg ttttatgaag
     3241 atgccaagga ggcatcacgt gtacttgaaa ttactttaac taaaagagat gctaaaaaag
     3301 aaaatccaat tccgatgtgt ggtgttccgt atcattctgc agatagttat atagatacac
     3361 ttgttaataa tggatataaa gtagctattt gtgaacagat ggaagatccg aaacaaacga
     3421 aaggtatggt tagacgtgag gtagtaagaa ttgtgactcc aggaactgtg atggagcaag
     3481 gtggtgtaga tgataaacaa aataactata ttttaagttt tgttatgaat caacctgaaa
     3541 ttgcgcttag ttactgtgat gtttctactg gcgaattaaa ggttacacat tttaatgatg
     3601 aagcgacttt attaaatgaa attacgacga taaaccctaa cgaagttgtt atcaatgaca
     3661 atatttccga taatttaaaa agacaaatta atatggtgac agaaacaata acagtcaggg
     3721 aaacgttatc atcagaaatc tatagtgtga atcaaactga acataaatta atgtatcaag
     3781 cgacacaatt attgctagat tatattcatc atacacaaaa acgtgattta tcgcatatcg
     3841 aggatgttgt tcaatatgca gctatagatt atatgaaaat ggatttttat gctaagagaa
     3901 accttgagtt aacggaaagc attcgattaa aatcaaaaaa aggaacgcta ctttggctaa
     3961 tggacgaaac gaaaacacca atgggagcac gccgcttaaa acaatggata gatagaccac
     4021 taataagtaa agaacaaatt gaagcacgat tagatatcgt tgatgaattt agtgctcatt
     4081 tcatagaaag agacacctta agaacatatc ttaatcaagt gtatgatatt gaacgtcttg
     4141 ttgggcgtgt tagttacgga aatgttaatg cgagagattt aattcaactt aaacattcca
     4201 tttctgaaat accgaatatt aaagcattac taaattctat gaatcagaat acgcttgtac
     4261 aagttaatca actagaaccc cttgatgatt tacttgatat attagaacag agtttagtag
     4321 aagaaccacc aatttcagtt aaagatggcg gactattcaa agttggtttt aatacgcaat
     4381 tagatgaata tcttgaagct tcaaaaaacg gaaaaacatg gttagcagaa ttacaagcca
     4441 aagaaagaca acgtacagga ataaaatcat tgaaaataag ctttaataaa gtgtttggtt
     4501 attttataga aataacacgt gccaacttgc aaaattttga accaagtgaa tttggttata
     4561 tgaggaagca aacgttatcg aatgctgaac gttttataac tgatgaactt aaagaaaaag
     4621 aagatatcat tttaggtgcg gaagacaaag ccatcgaatt agaatatcaa ttatttgttc
     4681 agctacgtga agaagttaaa aaatatactg aacgtttaca acaacaagct aaaattattt
     4741 cagagctaga ttgtttacag agctttgcag aaattgctca aaaatataat tacactaggc
     4801 cttcatttag tgaaaataaa acattagaat tagtggaatc taggcaccca gtagtggaaa
     4861 gagtaatgga ttataatgac tatgtgccta ataattgtcg attagataat gaaacattta
     4921 tatatttaat tacaggtccg aatatgtctg gtaaatcgac atatatgaga caagttgcca
     4981 taattagtat aatggcccaa atgggagctt atgtcccttg taaagaggca gtgttaccta
     5041 tatttgatca aatattcact agaataggtg cggcagatga tttggtttca ggtaagagta
     5101 cgtttatggt agaaatgcta gaagcacaaa aggcattaac ttatgcaaca gaggatagtt
     5161 tgattatttt cgatgaaatt ggacgtggta cttcaacgta tgacggttta gctttagcgc
     5221 aggcaatgat agagtatgta gctgaaacat cgcatgctaa aacgttattt tcaacacatt
     5281 atcatgaatt gacaacatta gatcaagcat taccaagtct aaaaaatgtt cacgtcgctg
     5341 ctaatgaata taaaggtgaa cttatattct tgcataaagt caaagatggt gcagttgacg
     5401 atagttatgg tattcaagtt gcgaaattag ctgatttacc tgaaaaagtt attagcagag
     5461 cacaagtgat cctaagcgag tttgaagcgt ctgctggtaa aaaatcatcg atatcaaatt
     5521 taaaaatggt cgaaaatgaa cctgaaatta atcaagaaaa tttaaactta agtgttgaag
     5581 aaacaactga tactttatct caaaaagact ttgaacaagc atcatttgat ttgtttgaaa
     5641 atgatcaaga aagcgagatt gaactacaaa ttaaaaattt gaatttatct aatatgacac
     5701 caattgaggc attggtgaag ttaagtgaat tacaaaatca attaaaatag aggtgttgca
     5761 aaatggggaa aattaaagaa ctccaaacct cattagcaaa taaaatcgca gcaggtgaag
     5821 tagttgaaag accgagttct gttgtgaaag aactgttgga aaatgctata gatgcaggcg
     5881 ctacagaaat aagcattgaa gtagaggaat ctggcgtcca atctattcgc gtagtcgata
     5941 atggaagcgg aattgaagcg gaagacttag gattagtatt tcatagacat gcgactagta
     6001 aattagatca agatgaagat ttatttcata ttaggacatt aggattccgt ggtgaagcac
     6061 tagccagtat ttcatcagtt gctaaagtaa cattgaagac ttgcacggat aatgctaatg
     6121 gaaatgaaat atatgtagaa aatggtgaaa tattaaatca taagcctgca aaagcgaaaa
     6181 aaggaacaga tatacttgta gaatcattat tttataatac accagcacgt ttaaaatata
     6241 ttaaaagttt atacactgaa ctaggtaaaa taacagatat tgtcaataga atggctatga
     6301 gccatccgga cattcgaata gcactcattt cagatggcaa aacaatgtta agtacaaatg
     6361 gttcaggacg aactaatgaa gtgatggcag agatttatgg gatgaaagtt gcacgagatt
     6421 tagtacatat atctggagat acaagtgatt atcacattga aggttttgtt gcaaagcctg
     6481 aacattctag aagtaataag cactatattt ctatttttat taatggacga tacattaaaa
     6541 actttatgct aaataaagcg attttagaag gctatcatac actcttaaca ataggtaggt
     6601 tcccgatttg ttatattaat attgaaatgg atccaatctt agtagacgta aatgttcatc
     6661 caacaaaact agaagtgcgt ttatcaaaag aagagcaact atatcaattg atagtgagca
     6721 aaatacaaga agcatttaaa gaccgtatat taattcctaa aaataacttg gattatgtgc
     6781 caaaaaaaaa taaagtatta tattcattcg aacaacaaaa aatcgaattt gaacaaagac
     6841 aaaacacaga gaataaccaa gagaagacgt tttcatctga agaaagtaac agtaagtcat
     6901 ttatggcaga aaatcaaaac gatgagatag ttataaaaga agattcatat aatccattcg
     6961 taacgaaaac atctgaaagt ttaataactg atgatgaatc ttctggttat aataatacac
     7021 gtgaaaaaga tgaagactac ttcaaaaaac aacaagaaat tttacaagaa atggatcaaa
     7081 catttgattc aaatgaggat gcatctgtgc aaaattatga gaataaagcg tctgatgatt
     7141 attatgatgt aaacgatatt aaaggaacaa aaagtaaaga ccctaaacga agaattccat
     7201 atatggaaat tgttggccaa gtacatggga cgtatattat tgctcaaaat gaatttggca
     7261 tgtacatgat tgaccagcat gcagctcaag aaagaataaa atatgaatat tttcgagata
     7321 aaataggtga agttaccaat gaagtacaag atttattaat cccgttaaca tttcattttt
     7381 caaaggatga acaattagtt attgatcaat ataaaaatga gcttcaacaa gtaggtatca
     7441 tgttagaaca ttttggtggt catgattata ttgtaagtag ctatccagtt tggttcccta
     7501 aagatgaagt agaagaaatc attaaagata tgattgagct aattttggaa gagaaaaaag
     7561 tagatattaa aaaattacgt gaagatgtag caatcatgat gtcatgtaaa aaatctatta
     7621 aagcgaatca ttatttacaa aaacatgaaa tgtctgattt aattgatcaa ttaagagaag
     7681 cggaagatcc atttacatgt ccacatggtc gtccaataat cattaatttt tcaaaatacg
     7741 aattagaaaa attatttaag cgtgtgatgt agagaggatg aatcaagtga ataacaacat
     7801 attgcctgct ataagaaaca ttaaagattt agagaaactg attaaaacag attataaaat
     7861 gtgtgtgctt ctagatatgc atataggaca tataaaaagt attatggaat tgctgaagca
     7921 aaatcatata gagtgtttta ttcatataga tttgataaaa ggtttaagcc acgatgaatt
     7981 tgcaagtgaa tttattattc agcaatacaa gccaaaaggt atcgtatcga ctaaatctaa
     8041 agtaataaaa aaagctaaat cattaaatac tttaacgatt tttagagtat ttattattga
     8101 tagtcaagca ttgaaacgca gtatagattt gataaaaaaa gtggaacctg attttgttga
     8161 agtacttcca ggtgttgcga gtaaagcgat tcatcatatt cagaaagaaa caaacacaca
     8221 agtcattgca ggtggcctaa ttaatacaat agatgaagtc aatgaagctg ttaaaaatgg
     8281 agcgaaatat gtaacaacta gttatgataa actttggtaa atcataagtc tgcaaaaatt
     8341 tcatttttca cgagatttag tcatataaca taataaaaaa gccgttttca tatgggatat
     8401 gtaataaggt atttaaggta tcatacaaag aataattaaa taacataatt agcacctcta
     8461 aaacgattaa aatcattaaa gttttagagg tgtttattag taaaaaatgc aactagtcat
     8521 aaaaaatatg gctgaagaat gtcaattaaa caaaatggcg tcataaataa attagtgcgg
     8581 attgatattt ctagttttca aattaaacta cttttataaa gacttgaaaa attaaatttg
     8641 acaaaatttt tacaaaaacc attgacaacg ctttcatatc gatagtatca taacaaatat
     8701 aataaaagtt aatacataga atagagacgg gagatttcta cgagccaaac tgctagtgta
     8761 ggaatctctt tgtctttttg ggaggacatt taatatgaat gtatatttag cagaattcct
     8821 aggaactgca atcttaatcc tttttggtgg tggcgtttgt gccaatgtca atttaaagag
     8881 aagtgctgcg aatggtgctg attggattgt catcacagct ggatggggat tagcggttac
     8941 aatgggtgtg tttgctgtcg gtcaattctc aggtgcacat ttaaacccag cggtgtcttt
     9001 agctcttgca ttagacggaa gttttgattg gtcattagtt cctggttata ttgttgctca
     9061 aatgttaggt gcaattgtcg gagcaacaat tgtatggtta atgtacttgc cacattggaa
     9121 agcgacagaa gaagctggcg cgaaattagg tgttttctct acagcaccgg ctattaagaa
     9181 ttactttgcc aactttttaa gtgagattat cggaacaatg gcattaactt taggtatttt
     9241 atttatcggt gtaaacaaaa ttgccgatgg tttaaatcct ttaattgtcg gagcattaat
     9301 tgttgcaatc ggattaagtt taggcggtgc tactggttat gcaatcaacc cagcacgtga
     9361 tttaggtccg agaattgcac atgcgatttt accaatagct ggtaaaggtg gttcaaattg
     9421 gtcatatgca atcgttccta tcttaggacc aattgccggt ggtttattag gtgcagtggt
     9481 atacgctgta ttttataaac atacatttaa tattggttgt gcaattgcaa ttgttgtagt
     9541 tattattact ttgattttag g
//
LOCUS       DAHHSC010000090         9512 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.091, whole genome shotgun sequence.
ACCESSION   DAHHSC010000090 DAHHSC010000000
VERSION     DAHHSC010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 9512)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9512)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9512
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.091"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            120..467
                     /gene="sarR"
                     /locus_tag="NBI57_001605"
     CDS             120..467
                     /gene="sarR"
                     /locus_tag="NBI57_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator SarR"
                     /protein_id="HCD1822545.1"
                     /translation="MSKINDINDLVNATFQVKKFFRDTKKKFNLNYEEIYILNHILRS
                     ESNEISSKEIAKCSEFKPYYLTKALQKLKDLKLLSKKRSLQDERTVIVYVTDTQKANI
                     QKLISELEEYIKN"
     gene            complement(656..1492)
                     /locus_tag="NBI57_001606"
     CDS             complement(656..1492)
                     /locus_tag="NBI57_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreD"
                     /protein_id="HCD1822546.1"
                     /translation="MDEQQWTGQLDLTVFFDGNRSVSRDIFFEKALKVIRPVYLNQST
                     IPTFYIVNVGGGYLDGDRYRMNVNVEDNAKVTLTSQGATKIYKTPSNHVEQYQTFNLK
                     DNAYLEYVADPIIAYENAKFYQHNTFNLNNSSSLFYTDILTPGYSKTGEAFKYQYMHL
                     INEIYIEDELVTYDNLLLNPNKQSINEIGYMEHYSHYGSAYFIHEDVNQKLIDSVYET
                     ISSYSNTFDCRVAISQLPTHGFAVRIFAYRTQIIEKILGTIQSYIAENIYDRKLDFLR
                     KY"
     gene            complement(1492..2106)
                     /gene="ureG"
                     /locus_tag="NBI57_001607"
     CDS             complement(1492..2106)
                     /gene="ureG"
                     /locus_tag="NBI57_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreG"
                     /protein_id="HCD1822547.1"
                     /translation="MANPIKIGIGGPVGAGKTQLIEKVVKRLSKEMSIGVITNDIYTK
                     EDEKILVNSGVLPESRIIGVETGGCPHTAIREDASMNFAAIDELLERHDDIELIFIES
                     GGDNLAATFSPELVDFSIYIIDVAQGEKIPRKGGQGMIKSDFFVINKTDLAPYVGASL
                     EQMAEDTKVFRGKRPFTFTNLKTDEGLDEVIDWIERDTLLKGLS"
     gene            complement(2119..2808)
                     /locus_tag="NBI57_001608"
     CDS             complement(2119..2808)
                     /locus_tag="NBI57_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreF"
                     /protein_id="HCD1822548.1"
                     /translation="MIDHTHLRLFQFCDSQFPTGAFSHSFGLETYIQRNIIHDDHTFI
                     AWLKMFLQEQLTYSDGLAMRLVYDALENDDTQKVLHIDKLMFVQNLPKETRVGAKQMG
                     TRMVKLALELYNSPWIAWYHQQMQDKKAKLNPAICFTMLGHHLGVDIETIIDYYLYQN
                     VSSLTQNAVRAIPLGQTAGQKIVTHMIPYIEETRKQIFELKEADFGMTAPGLELNQMA
                     HENVNVRIFIS"
     gene            complement(2801..3253)
                     /gene="ureE"
                     /locus_tag="NBI57_001609"
     CDS             complement(2801..3253)
                     /gene="ureE"
                     /locus_tag="NBI57_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreE"
                     /protein_id="HCD1822549.1"
                     /translation="MIVEEIQGNIANLSNSEKQKHVEKVYLENSDLVKRIQRVVTDHG
                     TEIGIRLKQPIDLQYGDILYADDHNMIIVDVNSEDLLVIQPRTLQEMGDIAHQLGNRH
                     LPAQFTETEMLVQYDYLVEDLLKSLGIPYVREDRKVNKAFRHIGHSHD"
     gene            complement(3266..4981)
                     /gene="ureC"
                     /locus_tag="NBI57_001610"
     CDS             complement(3266..4981)
                     /gene="ureC"
                     /locus_tag="NBI57_001610"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002447295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit alpha"
                     /protein_id="HCD1822550.1"
                     /translation="MSFKMTQNQYTSLYGPTVGDSIRLGDTNLFAQIEKDYAVYGEEA
                     TFGGGKSIRDGMAQNPRVTRDDVNVADLVISNAVIIDYDKVVKADIGIKNGYIFAIGN
                     AGNPDIMDNVDIIIGSTTDIIAAEGKIVTAGGIDTHVHFINPEQAEVALESGITTHIG
                     GGTGASEGSKATTVTPGPWHIHRMLEAAEGLPINVGFTGKGQATNPTALIEQINAGAI
                     GLKVHEDWGATPSALSHALDVADEFDVQIALHADTLNEAGFMEDTMAAVKDRVLHMYH
                     TEGAGGGHAPDLIKSAAFSNILPSSTNPTLPYTHNTVDEHLDMVMITHHLNAAIPEDI
                     AFADSRIRKETIAAEDVLQDMGVFSMISSDSQAMGRVGEVITRTWQVAHRMKEQRGPL
                     DGDFEHNDNNRIKRYIAKYTINPAITHGISEYVGSIEPGKLADIVLWDPIFFGVKPEL
                     VVKGGLINSAVNGDANGSIPTSEPMKYRKMYGQYGGNLTSTSMTFVSKTAYENGINRA
                     LNLKRMVRPVKNIRQLSKADMKNNSATPKLDVDPQTYEVYVDGEKITSNAATELPLTQ
                     RYFLF"
     gene            complement(4978..5388)
                     /locus_tag="NBI57_001611"
     CDS             complement(4978..5388)
                     /locus_tag="NBI57_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit beta"
                     /protein_id="HCD1822551.1"
                     /translation="MIPGEIITKSTEVEINNHHPETVIEVENTGDRPIQVGSHFHFYE
                     ANAALDFEREMAYGKHLDIPAGAAVRFEPGDKKEVQLVEYAGKRKIFGFRGMVNGPID
                     ESRVYRPTDENDAYAGVFGDNGAENVNKKGGKRS"
     gene            complement(5402..5704)
                     /locus_tag="NBI57_001612"
     CDS             complement(5402..5704)
                     /locus_tag="NBI57_001612"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001832402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit gamma"
                     /protein_id="HCD1822552.1"
                     /translation="MHFTQREQDKLMIVVAAEVARRRKARGLKLNHPEALALISDELL
                     EGARDGKTVAELMSYGRQILNKEDVMDGVEHMITDIEIEATFPDGTKLITVHHPIV"
     gene            5964..6869
                     /gene="yut"
                     /locus_tag="NBI57_001613"
     CDS             5964..6869
                     /gene="yut"
                     /locus_tag="NBI57_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016266007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urea transporter"
                     /protein_id="HCD1822553.1"
                     /translation="MKIIDVLLKNISQVVLISNKWTGLFILIGLFVADWTIGLAAIVG
                     SIIAYTFARFINYSEAEVNDGLAGFNPVLTAIALTIFLDKSGLDIVITMIATLLTLPV
                     AAAVREVLRPYKVPMLTMPFVIVTWFTILLSGQVKFVDTSLKLMPQNIETVNFSNNDR
                     IHFIQSLFEGFSQVFIEASVIGGVCILIGILIASRKAALLAVIASLLSFIIVALLGGN
                     YDDINQGLFGYNFVLMAIALGYTFKTAINPYISTFLGVLLTVVVQLGTTTLLEPFGLP
                     ALTLPFIIVTWILLFAGIKHDKVDA"
     gene            7086..8240
                     /locus_tag="NBI57_001614"
     CDS             7086..8240
                     /locus_tag="NBI57_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000999191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA/acyl-ACP dehydrogenase"
                     /protein_id="HCD1822554.1"
                     /translation="MNDMDNSFLITTEIQRKWIEKFKVIRDTFKAKAEYNDQHSQFPY
                     KNIEWLIKEGYGKLTLPKAYGGEGATIEDMVILQSFLGELDGATALSIGWHVSVVGQI
                     YEQKLWSQDMLEQFAVEINNGALVNRAVSEAEMGSPTRGGRPSTHAVKADDGYILNGV
                     KTYTSMSKALTHIIVAAYIEELESVGFFLVDRNLPGVEIADNWDVLGMRATESHDLIL
                     NDVKVPLKHLVETEKSKAPNGWILHIPSCYLGIAQAARNYAVDFAIQHSPNSIEGTIA
                     TLPTVQQNLGKMETLLLCARQFLWSTAKGYQQYKDDSQIRNPTSASKVMVMNQGLEVI
                     DLAMRIVGAKSLEMNRPLQRYYRDMRAGLHNPPMEDAAYTNIAKSITDTF"
     gene            8454..9362
                     /locus_tag="NBI57_001615"
     CDS             8454..9362
                     /locus_tag="NBI57_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCD1822555.1"
                     /translation="MKKLLLPLIIMLLVLAACGNQGEKNNKAETKSYKMDDGKTVDIP
                     KDPKRIAVVAPTYAGGLKKLGANIVAVNQQVDQSKVLKDKFKGVTKIGDGDVEKVAKE
                     KPDLIIVYSTDKDIKKYQKVAPTVVVDYNKHKYLEQQEMLGKIVGKEDKVKAWKKDWE
                     ETTAKDGKEIKKAIGQDVTVSLFDEFDKKLYTYGDNWGRGGEVLYQAFGLKMQPEQQK
                     LTAKAGWAEVKQEEIEKYAGDYIVSTSEGKPTPGYESTNMWKNLKATKEGHIVKVDAG
                     TYWYNDPYTLDFMRKDLKEKLIKAAK"
BASE COUNT         3191 a         1508 c         1647 g         3166 t
ORIGIN      
        1 aatttttata cttatttgtt tagaatgaac tttataacat agttggatag agtttcgatt
       61 taataaatta catgtgaacc ttgctacaac aagatgtgca tcagaggagt ggtttaataa
      121 tgagtaaaat taatgacatt aatgatttag tcaacgcaac atttcaagtt aagaagtttt
      181 tcagagatac aaaaaagaag ttcaatttga actatgaaga aatttatatt ttaaatcata
      241 ttttaagaag tgagtctaac gaaatctcat ctaaagagat tgctaagtgc tcagagttca
      301 aaccttacta tttaactaaa gctttacaaa agctaaaaga tttaaaattg ttatcaaaga
      361 aaagaagttt acaagacgaa agaacagtta ttgtttatgt tacagataca caaaaagcaa
      421 atattcaaaa actgatttca gaattagaag aatacattaa aaattaaatc aaggttaatt
      481 gcgtttaata acattgaacg ataacaattt attaaaacga agttatttat tcagcattgg
      541 gacataaaat taacttaaaa tttaaatatt gaagatgctt taattaaagt taaagactag
      601 ccatacctta tttcagctta ttaagcttga aacaaggtac actagtcttt ttattttaat
      661 attttcttag aaaatcaagt ttacgatcat aaatattttc tgcgatatag ctttggatgg
      721 ttccaagtat tttctctata atttgtgtgc gataagcaaa aattctaact gcaaagccat
      781 gtgtaggcaa ttgagaaata gcaacacgac aatcgaatgt attgctataa gaactaatgg
      841 tttcataaac tgaatcgatt agtttttggt taacatcttc atgtatgaag taagcagagc
      901 cataatgaga gtaatgttcc atataaccta tttcattgat cgattgttta ttaggattca
      961 ataataaatt atcatatgtg actaactcat cttcaatata aatttcattt attaaatgca
     1021 tatattgata cttgaaggct tcgccagttt ttgaataacc aggagttaaa atatcagtat
     1081 aaaataatga actagaatta ttgagattga acgtattgtg ttgataaaat ttagcatttt
     1141 catatgcgat gattggatca gcgacatatt ctaaatatgc gttatctttt aaattaaaag
     1201 tttgatactg ctcaacatga ttggaaggtg tcttgtatat ttttgttgca ccttgagatg
     1261 tcaatgtcac tttagcgtta tcttcgacat tcacattcat acggtaacga tctccatcta
     1321 aatagccacc acctacattt actatataaa atgtaggaat ggtagattga tttagataaa
     1381 ctggacgtat cactttaagt gctttttcaa agaaaatatc tcttgatact gatcgattgc
     1441 catcgaaaaa cactgttaaa tcaagttgcc cagtccattg ttgttcatcc attatgataa
     1501 tcctttgagt aaagtgtcgc gttcaatcca atcgataact tcatccaaac cttcgtcggt
     1561 ttttaagtta gtaaaagtaa atggacgttt accacgaaat actttagtat cttcagccat
     1621 ttgttctaat gatgcaccta catagggagc taaatcagtt ttgttaatta caaagaaatc
     1681 tgacttaatc ataccttgac cacctttacg tggaatcttt tcaccttgag caacatcgat
     1741 aatatatatt gaaaagtcaa caagttctgg gctaaatgtt gctgctaagt tatcgccacc
     1801 agattctatg aaaattagtt ctatatcgtc atgacgttct aataattcgt ctattgctgc
     1861 aaagttcata gatgcatctt cacgaatcgc agtatgagga catccaccag tttcaacacc
     1921 aatgatacga ctttcaggta gaactcctga atttactaat atcttttcgt cttcttttgt
     1981 atatatatca tttgtaataa cgccgatact catttctttt gaaagacgtt ttacaacttt
     2041 ttcaattaat tgtgttttac ctgcacctac aggaccacca ataccaattt taatcggatt
     2101 tgccacaatt ataacctcct atgatatgaa aattctaaca ttgacgttct catgcgccat
     2161 ttgatttagt tctaaaccag gcgctgtcat gccaaaatct gcttctttta attcgaaaat
     2221 ctgctttctt gtttcttcta tataaggaat catgtgagta actatctttt gaccagcagt
     2281 ttgtccaagt ggaatagcac gaacagcatt ttgagttaaa cttgaaacat tttgatataa
     2341 atagtaatca ataatcgttt caatatctac acctaaatga tggcctagca tagtaaaaca
     2401 aatagctgga tttaactttg ctttcttatc ttgcatttgt tgatgatacc aagcaatcca
     2461 tgggctatta taaagttcta aagccaattt aaccatgcga gtccccattt gttttgcacc
     2521 aacacgtgtt tctttaggta agttttgaac aaacatcagt ttatctatgt gtaatacttt
     2581 ttgtgtatca tcattttcca atgcatcata aactaaacgc atggctaaac catcagaata
     2641 ggtaagttgc tcttgtaaaa acatttttaa ccaagcaata aaagtatgat cgtcatgaat
     2701 tatatttcgt tgaatatatg tttcaagacc aaatgaatga ctgaaagcac ctgttggaaa
     2761 ctgtgaatca cagaactgaa ataatcttaa gtgtgtatga tcaatcatga gaatgcccta
     2821 tatgtctgaa agccttatta actttacggt cttctcgaac atatgggatg cctaaacttt
     2881 ttaataaatc ttcaactaaa taatcatatt gtactagcat ttcagtctct gtaaattgtg
     2941 ctggcaaatg acgatttcct aattgatggg ctatatctcc catttcttgc aatgttcttg
     3001 gttgaatcac taaaagatct tctgaattaa catccacaat aatcatatta tggtcatctg
     3061 cgtataaaat atctccatat tgtaagtcaa taggttgttt taaacgaatg cctatttcag
     3121 tgccatggtc tgtaacgact ctttgaatac gtttaacaag atctgaattt tcaaggtata
     3181 ctttttcgac gtgcttttgt ttttctgaat ttgacaaatt ggcaatattg ccttggattt
     3241 cttcaacaat cattctatgt tcctcctaga ataagaagta tctttgagtt aatggtaact
     3301 cagttgctgc attacttgta attttttctc catctacata tacttcatat gtttgtggat
     3361 caacgtctaa tttaggtgtt gcactgttat ttttcatatc tgctttagat aattgtctaa
     3421 tatttttaac tggacgcacc atgcgtttta aatttaatgc acgattgata ccattttcat
     3481 aagcagtttt agacacgaat gtcattgacg tacttgtaag gtttccgccg tattgaccat
     3541 acattttacg gtacttcatc ggttcagatg taggtataga accatttgca tcgccattta
     3601 cggcagagtt aattaatccg ccctttacaa ctaattcagg tttaacccca aagaaaattg
     3661 ggtcccataa gacaatgtca gctagtttgc ccggctcgat agatcctaca tattcagaaa
     3721 taccatgtgt aattgctggg ttaattgtat atttagcgat ataacgtttg atgcgattat
     3781 tatcattatg ttcaaaatca ccatctaaag gaccacgttg ttctttcatg cgatgtgcta
     3841 cttgccatgt tcgtgtaatt acttcaccta cacggcccat tgcttgtgaa tcggaactaa
     3901 tcatactgaa tacacccata tcttgcagaa catcttctgc tgcaatcgtt tctttacgaa
     3961 tacgtgaatc tgcgaatgcg atatcttcag gaatagccgc atttaaatgg tgagtaatca
     4021 ttaccatatc taaatgttca tctacagtat tatgtgtata aggcaaagtt ggatttgtag
     4081 atgaaggtaa aatatttgaa aatgcagcgg atttaattaa atcaggcgca tgaccgccac
     4141 cagcaccttc agtatggtac atatgaagta cacggtcttt aacagcagcc attgtgtctt
     4201 ccataaatcc tgcttcattt aaagtatctg catgtaatgc aatttgaaca tcaaattcat
     4261 cagcaacatc taatgcatga ctcaaagcag atggtgttgc accccagtct tcatgtactt
     4321 ttaatccaat tgctccggca ttgatttgtt caatgagtgc agttggattt gttgcttgtc
     4381 ctttacctgt aaaaccgaca ttaatcggta aaccttcggc agcttctaac attctatgaa
     4441 tatgccatgg acctggagtt acagttgttg ctttagaacc ttctgaagca ccagtaccac
     4501 caccaatatg agtcgtaata ccactttcta atgcgacctc tgcttgttca ggattaataa
     4561 aatgaacatg agtatcaata ccaccagcag tgacgatttt accttcagcg gcaatgatat
     4621 ctgttgttga acctataata atgtcgacat tatccattat atctgggttg ccggcattac
     4681 ctatggcgaa aatataacca tttttaatgc ctatatcagc tttaaccact ttatcgtaat
     4741 cgataataac ggcattagaa atgacaaggt ctgcaacgtt cacgtcatca cgtgttacac
     4801 gaggattttg cgccataccg tctctaatag atttaccacc accaaaagta gcttcttcac
     4861 cataaaccgc atagtctttt tctatttgag caaatagatt cgtatcacct aaacgaatag
     4921 aatctccaac agttggaccg tataagctcg tatattgatt ttgcgtcatt ttaaagctca
     4981 tgatcttttt cctccttttt tattcacgtt ttcagcaccg ttatctccga atacacctgc
     5041 atatgcatca ttttcatcag ttgggcgata gacacgtgac tcatcgatag gaccattgac
     5101 cataccacga aaaccaaaaa ttttacgttt gccagcatat tcaactaatt gaacttcttt
     5161 tttatcccca ggttcaaatc gaacagctgc tccagctgga atatctaaat gttttccata
     5221 tgccatttca cgttcgaaat ctaatgctgc atttgcttca taaaaatgaa aatgtgagcc
     5281 cacttgaatt ggtcgatctc ctgtattttc aacttcgata actgtttcag gatgatggtt
     5341 attaatttca acctctgtac tttttgtaat aatttctcct ggtatcattt gactgcctcc
     5401 tttaaacaat agggtgatgt actgtgatta acttagtacc atcggggaac gtagcctcga
     5461 tttcgatatc tgtaatcatg tgttcgacac catccatgac atcttctttg tttagaattt
     5521 gtctaccata actcattaac tctgcaacgg tcttaccatc gcgtgcacct tctaataatt
     5581 catcgctgat taaagctaat gcctcaggat gatttagttt caaaccacgt gctttacgac
     5641 gacgtgcaac ttccgccgcc actacaatca ttaatttgtc ttgctctcgt tgtgtaaaat
     5701 gcaaattaaa acccccaatt tcatattaga tacaatttac aaaatttata ttaatcctaa
     5761 ttgttgtgat aaacaagtaa tatacaaagt tcaatgtgta attagaaaat tatattttta
     5821 gcatatccgc tattgaagca aacaatctaa tcgaaaacaa atagcggaat atatttatgt
     5881 aaaaaccaaa atagttttta atataacttt tcatagaata gtagtatatt aatgagtaat
     5941 gattcaaagg aaaggtgaaa gatttgaaga taatagatgt gcttttgaaa aatatatctc
     6001 aggttgtgtt aattagtaat aaatggacag gattatttat cttaatagga ttatttgtag
     6061 ccgattggac aattggatta gcggctattg taggtagcat catcgcctat acttttgcgc
     6121 gttttataaa ttatagtgag gcagaggtta atgatgggtt agccggattt aatccagtgt
     6181 taactgccat tgcgttaaca atctttttag ataagtcagg attagatatt gttataacaa
     6241 tgatagcaac tttattaacg ttaccagttg ctgctgcagt gagagaagtt ttaagaccat
     6301 ataaagttcc gatgctgacg atgccttttg tcattgtgac ttggtttaca attttacttt
     6361 caggacaggt taaatttgta gatacatcgt taaagttaat gcctcaaaac attgaaacgg
     6421 ttaattttag caacaatgat agaatacatt tcattcagtc attatttgaa ggattcagtc
     6481 aagtatttat cgaagcgagt gtaattggtg gcgtatgtat tttaatcggc atattgatag
     6541 catcaagaaa agcagcactc ttagctgtta tagctagttt gttaagcttt atcattgtag
     6601 ctctattagg tggtaattat gatgatatta atcagggatt attcggttat aactttgtat
     6661 taatggcaat cgcactagga tatacattta aaacagcgat taacccttat atttcgactt
     6721 ttttaggtgt gttattaaca gtagtggtgc aactaggtac aacaacattg cttgaaccgt
     6781 ttggcttacc tgcattaaca ttgccattta ttatcgtgac atggatttta ttatttgctg
     6841 gtattaaaca tgacaaagta gatgcttgat agttaaatca aacctaatat tgtttgaata
     6901 tcaccttaaa ccatacagcg aattgtatag tttaaggtgt atttttatgg ataaaattaa
     6961 gtgcatactt aatcgaatta tcagttttct tttaattgtt cacagcacgt tcctttttat
     7021 ttttaaagtc gagtgttaaa ctaggaataa ataatttata ttgtgtgttg tgtggggtga
     7081 ctaatatgaa tgatatggat aattcctttt taataacaac ggaaattcaa agaaaatgga
     7141 ttgaaaaatt caaagtaatt agagatacat ttaaggctaa agctgaatat aatgatcaac
     7201 atagccaatt tccatataaa aatattgaat ggttaattaa agaaggttat ggaaaattaa
     7261 cgttaccaaa agcatatggt ggtgaaggtg cgaccataga agacatggtt attttgcaat
     7321 catttttagg cgaacttgat ggtgccacag cattatctat tggttggcat gtgagtgtcg
     7381 taggacaaat ttatgaacag aaattatggt ctcaagatat gttggagcaa tttgctgttg
     7441 aaattaataa tggtgcatta gttaatagag cagttagtga agctgaaatg ggtagtccaa
     7501 caagaggggg aagaccaagt acacatgctg ttaaagctga tgatgggtat attttaaatg
     7561 gtgtgaagac atatacatca atgagtaaag cactaacaca tattattgtt gctgcttata
     7621 tagaagaatt agaaagtgtt ggttttttct tagtagacag gaatttgcct ggtgttgaaa
     7681 ttgccgataa ttgggacgta ttaggtatgc gagcgacaga aagtcatgat ttaatattaa
     7741 atgatgtaaa agtaccatta aaacatttag tggaaacaga gaaaagtaaa gcgccaaatg
     7801 gttggatatt gcatatacca agctgttatt taggtatagc acaagctgct agaaattatg
     7861 ctgtagactt tgcaatccaa catagcccta atagtattga aggaacgatt gcaacattgc
     7921 caactgtgca acaaaattta gggaaaatgg aaacgctatt gttatgtgcg agacaatttt
     7981 tatggagtac agcaaaaggg tatcaacaat ataaagatga cagtcagata agaaatccaa
     8041 caagtgcgag taaagtgatg gtaatgaatc aagggcttga agtgattgat ttagcgatga
     8101 gaatagttgg tgctaaaagc ttagaaatga atcgaccatt acaacgttac tatcgtgaca
     8161 tgcgtgcagg attacacaat ccacctatgg aagatgcagc atatacaaat attgctaaga
     8221 gtattacaga tacattttaa atttaaaaag gtaaatcgat cgttgattgg cataaagaga
     8281 tgaaatttga cgttcggatt tacccttttt tgtggggatt tatagggatg ttaagatatt
     8341 ttgctaaatt ttttgcgaaa ataaaaaaat aaatagcact agctattgta aatgtatatg
     8401 caatcttgta taattttgga ataattgata atcattttca ataggaggaa attatgaaaa
     8461 aactattatt accattaata attatgttat tagtgttagc tgcgtgtggg aaccaaggtg
     8521 aaaaaaataa caaagctgaa actaaatctt ataaaatgga cgatggcaaa acggtagata
     8581 ttccgaaaga ccctaaacgc attgcagtag ttgcgccaac atatgctggt ggacttaaaa
     8641 aattaggtgc aaacattgta gctgtaaatc aacaagtcga tcaaagcaaa gtattaaaag
     8701 ataaatttaa aggtgttaca aaaattggtg atggcgatgt agaaaaagtt gctaaagaaa
     8761 agccagattt aattattgta tactctactg acaaagatat taaaaaatat caaaaagtag
     8821 caccaacagt agttgttgac tataataagc ataaatattt agaacaacaa gaaatgttag
     8881 ggaaaattgt tggtaaagaa gataaagtaa aagcttggaa gaaagattgg gaagaaacaa
     8941 ctgctaaaga cggtaaagaa attaaaaaag caattggaca agatgtaaca gtgtcattgt
     9001 ttgatgaatt tgataaaaaa ttatacactt acggcgataa ctggggtcgt ggtggagaag
     9061 tattatatca agcatttggt ttgaaaatgc aaccagaaca acaaaagtta actgcaaaag
     9121 caggttgggc tgaagtgaaa caagaagaaa ttgaaaaata tgctggtgat tacattgtga
     9181 gtacaagtga aggtaaacct acaccaggat atgaatcaac aaacatgtgg aagaatttga
     9241 aagctactaa agaaggacat attgttaaag ttgatgctgg tacatactgg tacaacgatc
     9301 cttatacatt agatttcatg cgtaaagatt taaaagaaaa attaattaaa gctgcaaaat
     9361 aattcagcta tataagttag tgaaatgaga gtctgaaaca tatcatcttt tgatattgta
     9421 ttaggctctt atttttatag ctagaaagtt agatatttgt atttttttaa ataataagtg
     9481 ccgttgttat cgttcaattt aattaatgat ag
//
LOCUS       DAHHSC010000091         9133 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.092, whole genome shotgun sequence.
ACCESSION   DAHHSC010000091 DAHHSC010000000
VERSION     DAHHSC010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 9133)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9133)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9133
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.092"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            complement(215..721)
                     /locus_tag="NBI57_001616"
     CDS             complement(215..721)
                     /locus_tag="NBI57_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1822556.1"
                     /translation="MKKLLTASIIACSVVMGVGLVNTSAEAASGNSIDTVKQLIKGDQ
                     SLENVKIGESIKDVLTKYKNPMYSYNEDGTEHYYEFHTKKGMLLVTTDGKKNNGKVTH
                     ISMMYNDANGPTYQAVKNYVGKAVTHTEYSKVAGNFGYIEKGKTTYQFASAPKDKNIK
                     LYRIDLEK"
     gene            complement(857..1657)
                     /locus_tag="NBI57_001617"
     CDS             complement(857..1657)
                     /locus_tag="NBI57_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCD1822557.1"
                     /translation="MDLFTRKDGTSIHYSTLGEGYPIVLIHTVLDNYSVFNKLAAQLA
                     KSFQVVLIDLRGHGYSDKPRHIEIKDFSDDIVELLKYLYIEEVAFVCHEMGGIIGADI
                     SVRYPEFTSSLMLVNPTSIEGELPEERLFRKYAHIIRNWDPEKQDKFLNKRKYYRPRK
                     MNRFLKHVVDTNEISTKEEIQAVKEVFKNADISQTYRNVVVPTKIIAGEFGERTTRLE
                     AKEVADLIQNADFEVYQESSAFPFVEEQERFVEDTASFINKHHDEKHV"
     gene            complement(1641..2360)
                     /locus_tag="NBI57_001618"
     CDS             complement(1641..2360)
                     /locus_tag="NBI57_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HCD1822558.1"
                     /translation="MDLNQIKAVVFDLEGTLLDRVKSREKFIEEQYERFHDYLIHVQL
                     ADFKKAFIELDDDEDNDKPDLYKEIIKRFHVDRLTWKDLFNDFEMHFYRYVFPYYDTL
                     YTLEKLSQKGFQIGVIANGKSKIKQFRLHSLGLMHVINYLSTSETVGFRKPHPKIFED
                     MIDQLGVLPEQIMYVGDDALNDVAPARAMGMVSVWYKQEDAEIEPLEEEVDFTITTVE
                     ELLTILPIKNDNKGENYGSIY"
     gene            complement(2415..3365)
                     /locus_tag="NBI57_001619"
     CDS             complement(2415..3365)
                     /locus_tag="NBI57_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HCD1822559.1"
                     /translation="MTFNKVLLSWIVILIITTSIYLFWQLGDINDVFNQSILINVRLP
                     RLLEALLTGMILTVAGLIFQTVLNNALADSFTLGLASGATFGSGLALFLGLTTLWIPV
                     FSITFSLITLITVLVITSVLSQGYPVRILILSGLMIGALFNSLLYFLILLKPRKLNTI
                     ANYLFGGFGDAEYSNVSIIAITFIIALFGIFIILNQLKLLQLGELKSQSLGLNVQLIT
                     YIALCIASMITAINVAYVGIIGFIGMVIPQLIRKWQWKQSLGRQLALNIVIGGQIMVM
                     ADFIGSHILSPVQIPASIIIALIGIPVLFYMLISQSKRLH"
     gene            complement(3514..4401)
                     /locus_tag="NBI57_001620"
     CDS             complement(3514..4401)
                     /locus_tag="NBI57_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015581799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCD1822560.1"
                     /translation="MKKSLIAFILIFMLVLSGCGMKDNDKQGSDDNGSSKSPYHRIVS
                     LMPSNTEILYELGLGKYIVGVSTVDDYPKDVKEGKKQFDALNLNKEELLKAKPDLILA
                     HESQKATANKVLSSLEKQGIKVVYVKDAQSIDETYNTFKQIGKLTHHDKQAEQLVEET
                     KDNIDKVIDSIPAHHKKSKVFIEVSSKPEIYTAGKHTFFNDMLEKLEAQNVYSDINGW
                     NPVTKESIIKKNPDILISTEAKTRSDYMDIIKKRGGFNKINAVKNTRIEVVNGDEVSR
                     PGPRIDEGLKELRDAIYRK"
     gene            complement(4653..5288)
                     /locus_tag="NBI57_001621"
     CDS             complement(4653..5288)
                     /locus_tag="NBI57_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III domain-containing protein"
                     /protein_id="HCD1822561.1"
                     /translation="MLGTDELYKLLYRHMGPQNWWPADNDIELMLGAILVQNTRWRNA
                     EIALNQIKEHTHFNPNHILELPIETLQSLIHSSGFYKSKTLTIKTLLTWLARHHFNYQ
                     EINERYKGGLRKELLSLKGIGSETADVLLVYIFGRIEFIPDSYTRKIYDKLGYENTKN
                     YDQLKKVVTLPNHFTNQDANEFHALLDVFGKHYFRDKDIKNYDFLEPYFKK"
     gene            complement(5558..7219)
                     /locus_tag="NBI57_001622"
     CDS             complement(5558..7219)
                     /locus_tag="NBI57_001622"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HCD1822562.1"
                     /translation="MNIIDQVKQTLVEEIAASINKAGLADEIPDIKIEVPKDTKNGDY
                     ATNIAMVLTKIAKRNPREIAQAIVDNLDTEKAHVKQIDIAGPGFINFYLDNQYLTAII
                     PEAIEKGDQFGHVNESKGQNVLLEYVSANPTGDLHIGHARNAAVGDALANILTAAGYN
                     VTREYYINDAGNQITNLARSIETRFFEALGDNSYSMPEDGYNGKDIIEIGKDLAEKHP
                     EIKDYSEEARLKEFRKLGVEYEMAKLKNDLAEFNTHFDNWFSETSLYEKGEILEVLAK
                     MKELGYTYEADGATWLRTTDFKDDKDRVLIKNDGTYTYFLPDIAYHFDKVKRGNDILI
                     DLFGADHHGYINRLKASLETFGVDSNRLEIQIMQMVRLMENGKEVKMSKRTGNAITLR
                     EIMDEVGVDAARYFLTMRSPDSHFDFDMELAKEQSQDNPVYYAQYAHARICSILKQAK
                     EQGIEVTAANDFTTITNEKAIELLKKVADFEPTIESAAEHRSAHRITNYIQDLAAHFH
                     KFYNAEKVLTDDIEKTKAHVAMIEAVRITLKNALAMVGVSAPESM"
     gene            complement(7216..7632)
                     /locus_tag="NBI57_001623"
     CDS             complement(7216..7632)
                     /locus_tag="NBI57_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000359087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HCD1822563.1"
                     /translation="MDKKVSIQTKQVLKQHNEKEKFEFTTEGTWQQRQSNFIRYVEQI
                     EDATVNVTIKVDDDSVKLIRKGDINMNLHFVEGQTTTTFYDISAGRIPLEVKTLRILH
                     FVSGDGGKLKIHYELYQDNEKMGSYQYEINYKEIGE"
     gene            complement(7725..7833)
                     /locus_tag="NBI57_001624"
                     /pseudo
     CDS             complement(7725..7833)
                     /locus_tag="NBI57_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499172.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(7908..8918)
                     /gene="adhP"
                     /locus_tag="NBI57_001625"
     CDS             complement(7908..8918)
                     /gene="adhP"
                     /locus_tag="NBI57_001625"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
                     /protein_id="HCD1822564.1"
                     /translation="MRAAVVTKDHKVSIEDKKLRALKPGEALVQTEYCGVCHTDLHVK
                     NADFGDVTGVTLGHEGIGKVIEVAEDVESLKIGDRVSIAWMFESCGRCEYCTTGRETL
                     CRSVKNAGYTVDGAMAEQVIVTADYAVKVPEKLDPAAASSITCAGVTTYKAVKVSNVK
                     PGQWLGVFGIGGLGNLALQYAKNVMGAKIVAFDINDDKLAFAKELGADAIINSKDVDP
                     VAEVMKLTDNKGLDATVVTSVAKTPFNQAVDVVKAGARVVAVGLPVDKMNLDIPRLVL
                     DGIEVVGSLVGTRQDLREAFEFAAENKVTPKVQLRKLEEINDIFEEMENGTITGRMVI
                     KF"
BASE COUNT         2858 a         1759 c         1158 g         3358 t
ORIGIN      
        1 tgattaaagt aatcttctat attttctagt aaaattttat cgttaatacg cattggtagt
       61 actcctttaa aatgaaacat ctttatttat tgtattggtt taatttcccc ttcgcaaatc
      121 ataaaaatta atattcactg tattatgcca tgtaataaaa taaccgacaa gtattgcgat
      181 ttaacttgtc ggttaagcac gattaattta attattattt ttccaaatca atacgatata
      241 attttatgtt tttatctttt ggtgctgagg caaattgata agtcgttttg cctttttcaa
      301 tatatccaaa attaccagca actttgctat attccgtatg tgttactgct ttgccaacat
      361 aatttttaac agcttgatat gttggaccat tagcatcatt atacatcatt gaaatatgag
      421 ttactttacc attgtttttc ttaccatcag tagttactaa taacatacct tttttagtat
      481 ggaattcgta ataatgttca gttccatctt cattgtaaga atacatagga tttttgtact
      541 tagttaaaac atctttaata gattcgccaa ttttcacatt ttctaatgac tgatcaccct
      601 taattaattg tttaacagta tcaatagagt tgccacttgc tgcttcggca ctagtgttca
      661 ctaagcctac tcccattaca acagaacatg caattatact tgccgttagt agctttttca
      721 taagcactct ctccttttat ttatatcgtc ttgtgctcac aaccattata caacaatcat
      781 ttctaaaaca acagtcatat tacaattata ttacaaataa taattactac ttttatattt
      841 cacttatcac taaaaattaa acatgctttt catcgtgatg tttgttgata aatgaagctg
      901 tatcttcgac gaatctttct tgctcttcaa caaatgggaa tgcacttgat tcttgatata
      961 cttcaaagtc cgcattttgg attaaatcag ctacttcttt agcttccaat cttgttgttc
     1021 tttcaccgaa ttctcctgca ataatttttg tcggtactac gacatttcta taagtttgag
     1081 aaatatcagc gtttttgaat acctctttaa ctgcttgaat ttcttcttta gttgatattt
     1141 catttgtatc tacgacatgt ttaaggaatc gattcatttt tctcggacga taatacttac
     1201 gcttatttaa aaatttatct tgtttttcag gatcccagtt tcgaataata tgggcatatt
     1261 ttctaaataa acgttcttcc ggtaattcac cttcaataga tgttggattt accaacataa
     1321 gtgatgatgt aaattcagga taacgtactg aaatatccgc accaatgatt ccacccattt
     1381 catggcatac aaatgcaact tcttcaatgt ataaatattt aagtaattca acaatgtcat
     1441 cagaaaaatc ttttatttca atgtgacgag gtttatcaga atagccatgt ccacgtaaat
     1501 caattaacac aacttgaaat gattttgcta attgtgctgc taatttatta aacacagaat
     1561 aattatcaag tacagtatga atcaatacga taggatagcc ttcacctaat gtactgtaat
     1621 gtatcgatgt tccatctttt ctagtaaata gatccataat tttctccttt attatcattt
     1681 tttattggta aaatggttaa taattcttcc actgttgtaa ttgtaaaatc aacttcttct
     1741 tcgagtggtt caatttcagc atcttcttgt ttataccata cactaaccat gcccatagct
     1801 cgtgctggag ctacatcatt taacgcatca tcgccaacat acataatttg ctcaggtaat
     1861 acccctagtt gatcaatcat atcttcaaaa attttaggat gtggtttacg aaaaccaact
     1921 gtttctgatg ttgataaata attaataaca tgcatcaaac caagtgaatg taatcgaaat
     1981 tgtttaatct tagatttacc atttgcgata acaccaattt gaaagccttt ttgcgatagc
     2041 ttttctagtg tatacaaagt atcgtaataa ggaaatacat aacgataaaa atgcatttca
     2101 aaatcattaa ataagtcttt ccaagttaac ctatctacat ggaaacgttt aatgatttct
     2161 ttatataaat caggtttatc attatcttca tcgtcatcta gctcaataaa tgctttttta
     2221 aaatctgcca gttgaacatg aattaagtag tcatgaaatc gttcatattg ctcttcgata
     2281 aatttctctc gagatttaac tctgtccaac aacgtacctt ctaaatcaaa tacaactgct
     2341 ttaatttgat tcaaatccat cgcttatgcc ctctttatgt ttatagttat ttttatttta
     2401 gcaaatgtca tgtgctagtg taaccgtttc gactgagata ttagcatgta aaataacact
     2461 ggtataccaa ttaatgcaat aataatgctt gccggtattt gtactggtga cagtatatgg
     2521 ctaccaataa aatctgccat aaccattatt tgtcctccaa ttacaatatt caaagccaat
     2581 tgtcttccta atgattgttt ccactgccat tttctaatga gttgtggtat caccatacca
     2641 atgaatccaa tgatgccaac atatgcgaca tttatcgccg ttatcataga agctatacat
     2701 aacgcgatat atgtaatcaa ttgaacattt aagcctagtg actgactttt tagttctcct
     2761 aattgcaata actttagttg attaagaatg ataaatatac caaacagtgc aatgataaat
     2821 gtgattgcta ttatggatac atttgagtat tctgcatcac caaagccacc aaacaaataa
     2881 ttggcaattg tatttaattt gcgaggtttt aataaaatca aaaaatatag aagtgaattg
     2941 aataatgcac caatcattaa accacttaat attaagattc taactggata gccttggctc
     3001 aataccgacg taatgactaa tacagttatt aatgttatca aactaaatgt tattgaaaat
     3061 acaggaatcc ataacgttgt taaacctaaa aataatgcta atcctgaacc aaatgtagca
     3121 ccgcttgcca atcctaatgt aaagctatct gccaatgcat tatttaaaac tgtttgaaat
     3181 ataagacctg caactgttaa tatcatacct gtcaacaatg cttctaataa tctcggtaat
     3241 ctaacattga ttaaaataga ctggttaaat acatcattga tatcccccaa ctgccaaaat
     3301 agatatatgc tagttgttat aatcaatatg actatccagc tcaataatac tttattaaat
     3361 gtcatagcat tctcttttct ttcatcgata attacattca ttatagcaat cacatactcg
     3421 cgttgctatc attcactaaa tatattttaa aaacccctta tctcaaacat gtattttttt
     3481 acatgtagca ataaggggca taattagaat ggtttatttt ctataaattg catctcttaa
     3541 ttcttttaat ccttcatcaa tacgtggacc tggtcttgat acttcatcac catttacaac
     3601 ttcaatacgt gtattcttga cagcattaat tttattgaat ccaccccttt ttttgatgat
     3661 atccatataa tctgatcttg tcttagcttc cgtcgaaatt aatatatctg ggttcttttt
     3721 aataatactt tccttcgtta cagggttcca accattaatg tcactataaa cattttgggc
     3781 ttctaatttt tctaacatat cattaaaaaa tgtatgcttc cctgctgtat atatttcagg
     3841 ctttgatgaa acctcaataa atacttttga ttttttatga tgagcaggaa ttgaatctat
     3901 gactttatcg atattatctt tagtttcctc aacaagttgt tcagcctgct tatcatgatg
     3961 cgttaatttc ccaatttgct taaatgtgtt gtatgtttca tcaattgatt gtgcatcttt
     4021 aacatacact actttgatgc cttgtttctc taatgatgac aatactttat tagcagttgc
     4081 cttttgcgac tcatgcgcaa gaattagatc tggctttgcc tttaaaagtt cctctttatt
     4141 tagattcaaa gcatcaaatt gtttcttacc ctctttcaca tcttttggat aatcatcaac
     4201 cgttgaaaca ccaactatgt atttacctaa tcctaattca tataaaattt cagtattact
     4261 aggcattaac gaaacaattc tatggtacgg cgatttagac gagccattat catcgctacc
     4321 ttgtttatca ttatctttca taccacagcc actcaggaca agcataaaaa tcaaaataaa
     4381 agcaattaac gatttcttca cattatcaca tctccttttt aatcccactt ttcgctttag
     4441 cgattagagg gatttatgca gttgtgcctt agcatccaac tgcgttcctc ttcaccttta
     4501 gtgattagag gcttttatgc agttgctgca caaacaaaca actgtttttt tctttatatg
     4561 cttctattta ttatataagt ccacatacta aaagaaaata gtaaattatt ttttatattt
     4621 catataaact catctatcta acttcacagc gtttactttt taaagtaagg ttctaaaaaa
     4681 tcataattct ttatatcttt gtctctaaag taatgtttac caaatacatc taacagagca
     4741 tgaaattcat tagcatcttg atttgtaaaa tgatttggta atgtgactac tttttttaat
     4801 tgatcataat ttttagtgtt ttcatatcct aatttatcat atatttttct tgtatagcta
     4861 tctggaataa attcaatacg tccgaatata taaacaagta agacatctgc tgtttcactt
     4921 ccaatacctt tcaaagataa taattctttt cttaatccac ctttatatcg ctcattaatc
     4981 tcttgataat tgaaatgatg tcgtgctaac catgttaata atgttttaat cgtcagtgtt
     5041 ttacttttat aaaagccact tgaatgtatc aatgattgta acgtttcaat aggtagttct
     5101 aatatatgat tgggattaaa atgcgtatgt tctttaatct gattcaatgc aatttctgca
     5161 tttcgccatc tagtattttg aactaaaatt gcacctaaca tcagttcaat gtcattatca
     5221 gcaggccacc aattttgcgg tcccatatgc ctatataaca atttatacaa ttcatcagtt
     5281 cctaacattt acaccctcct tttagagccc ctaattaata aattaattag gggctcttat
     5341 gcagttgctg cgcaagcaag ccaactgtat tcctttactc cccttgagct ttagcgattt
     5401 aggctctctt atgcagttgt gcagtagcat ccaactgcgt ccctcttaac cttattcaca
     5461 tactaagcat tccccaatat gtcacatacc taattaaaaa cactccaaca caaagtatct
     5521 ttcgagaaac tctacgttgg agtgtatata aatgttctta cattgattca ggtgcgctta
     5581 caccgaccat tgccaatgca tttttcaatg taattctgac cgcttcaatc atagcaacat
     5641 gtgcttttgt tttttcaata tcatctgtta acactttttc agcattatag aatttatgga
     5701 aatgagcagc caagtcttga atatagttag taattctatg tgctgatcta tgctcagcag
     5761 cactttcaat tgtaggttcg aaatcagcta cttttttcaa caattcaatc gctttttcat
     5821 ttgtaatcgt tgtaaaatca ttcgcagcag tcacttcaat accttgctct ttcgcttgtt
     5881 ttaaaattga acaaatacgc gcatgtgcat attgagcata gtaaactgga ttgtcttgag
     5941 attgctcttt cgctaattcc atatcaaaat caaagtgact atcaggacta cgcatagtta
     6001 agaaataacg tgcagcgtca acgccaactt cgtccataat ttctcttaat gtaatcgcat
     6061 taccagtacg tttactcatc ttcacttctt taccattttc cattaaacga accatttgca
     6121 tgatttgaat ttctaaacga ttactatcta caccaaacgt ttcaagagat gctttcaaac
     6181 gattaatata accatgatga tcagcaccaa ataaatcgat taaaatgtca ttaccacgtt
     6241 taactttatc gaagtggtac gcaatatctg gtaagaaata cgtatatgta ccgtcatttt
     6301 taattaatac tctgtctttg tcgtctttaa aatcagttgt acgtaaccat gtagcgccat
     6361 cagcttcata cgtataacct aattctttca tttttgctaa aacttcaaga atttcgcctt
     6421 tttcatataa agatgtttca ctaaaccaat tatcaaaatg cgtattgaac tctgctaaat
     6481 catttttcaa tttagccatt tcgtattcta cgcctaattt tctaaattct ttcaaacgtg
     6541 cttcttcaga ataatcttta atttcagggt gtttctctgc taaatcttta cctatttcaa
     6601 taatatcttt tccattatag ccatcttctg gcattgaata actattgtca cctaaagctt
     6661 caaagaaacg tgtttcaatc gaacgcgcta agttagtaat ttgattacca gcatcattaa
     6721 tataatattc acgtgttaca ttatagccag ctgcagttaa aatattagct aaagcatcac
     6781 caactgctgc atttctagca tgaccaatat gtaaatctcc tgtagggtta gctgaaacat
     6841 actcaagcaa tacattttga ccttttgatt catttacatg tccaaattga tcaccttttt
     6901 caattgcttc aggaataatt gctgttaaat actgattatc taagtaaaaa ttaatgaatc
     6961 ctggaccagc aatgtcaatt tgttttacat gtgctttttc agtatctaag ttatcaacaa
     7021 tcgcttgagc aatttcacga ggattacgct ttgcaatctt agtcagtacc atcgcaatat
     7081 tagtagcata atctccattt tttgtatctt taggaacttc aattttaata tcaggaatct
     7141 catctgctaa tcctgctttg ttaatacttg ctgcaatttc ttctactaat gtttgtttca
     7201 cttgatcaat aatattcatt cgcctatctc cttatagtta atttcatatt gataagaacc
     7261 cattttttca ttatcttgat ataattcata atgaatcttt agcttgccac cgtctccact
     7321 tacgaaatgt aaaatgcgta atgttttaac ttctagtgga attcgtccag ccgatatatc
     7381 gtaaaaagtt gttgtcgttt gtccttcaac aaaatgcaaa ttcatattaa tgtcgccttt
     7441 acgaatcaac ttaacgctat catcatccac ttttattgta acattaactg ttgcatcctc
     7501 aatttgttct acataccgaa taaagttaga ttgcctttgt tgccaagttc cttcagtagt
     7561 aaattcaaat ttttcttttt cgttgtgctg tttcaacact tgctttgttt gaatacttac
     7621 ttttttatcc aatttactac tcaccttaat cttcgcataa tagaaatatt atagcataaa
     7681 ataaggtcat cttgttatgc cttcttaaag ctaaataggt tcaattaatt aacaattcca
     7741 tacttaatca acgcttcata tatgtaagcc attttataaa atacaaacaa cacctcaaaa
     7801 ccaaaaaaac acgagatttt cactatcttt catgaaaacc tcgtgtttga ttataatgat
     7861 agtgttcaga gcactacctt ctttatctat atagtcagtt gatattttta aaatttaata
     7921 accattctac cagttatagt accattttcc atttcttcaa aaatatcatt gatttcttct
     7981 aattttctta attgaacttt aggtgttact ttattttcag cagcaaattc aaacgcttca
     8041 cgtaagtctt gtcttgtacc aacaagtgaa cctactactt caataccgtc aagcactaat
     8101 cttgggatat ctaagttcat tttatcaaca ggtaaaccaa cggcaacaac tcttgcacca
     8161 gcttttacaa catcaaccgc ttggttaaat ggcgtcttag caactgaagt taccactgtt
     8221 gcatctaatc ctttgttatc agttaatttc ataacttctg caactggatc aacatcttta
     8281 gaattaataa tagcatctgc acctaattct ttcgcgaatg ctaatttatc atcattaatg
     8341 tcgaaggcaa caattttagc ccccataacg tttttagcat attgtaaagc taggttacct
     8401 aaaccaccta taccaaaaac acctaaccat tgaccaggtt ttacattact tacttttaca
     8461 gctttataag ttgtcacacc tgcgcatgta atagaagacg ctgctgctgg atctaatttt
     8521 tcaggtactt tcacagcata gtctgcagta acaataactt gttcagccat tgcaccatct
     8581 actgtataac cagcattttt cacactacgg caaagtgttt cacgacctgt tgtacaatat
     8641 tcacatcttc cacagctttc gaacatccaa gcgatagaca cacggtctcc aatttttaat
     8701 gattctacat cttctgcaac ttcgatgact ttaccaatac cttcatgacc taaagtaacg
     8761 cctgtaacat caccaaaatc agcattctta acatgtaaat cggtatgaca aacaccacaa
     8821 tattccgttt gtaccaatgc ttcaccaggt tttaaagctc ttaacttttt gtcctcaata
     8881 cttactttgt gatctttcgt tacaactgct gctctcataa taatgtcctc cttatagtca
     8941 ctccagtatg tatgatatta attggcgatt actttcaaaa atgaaaacga ataattatag
     9001 atgtaatttt tatttgtaat agacgccaga ttaaaacata ctactgtgac tttgttttta
     9061 gttagatatc aaccgttaac ttttaacgtt tccctagtta atatgttaaa agaaatgaca
     9121 taatcgatat gct
//
LOCUS       DAHHSC010000092         9001 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.093, whole genome shotgun sequence.
ACCESSION   DAHHSC010000092 DAHHSC010000000
VERSION     DAHHSC010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 9001)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9001)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9001
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.093"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            complement(249..2255)
                     /gene="ggt"
                     /locus_tag="NBI57_001626"
     CDS             complement(249..2255)
                     /gene="ggt"
                     /locus_tag="NBI57_001626"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HCD1822565.1"
                     /translation="MVINLNDKQTKTSKEGLISVSHPLAAKIGKDVLDQGGNAMDAVI
                     AIQLALNVVEPFASGIGGGGYLLYYEQSTGSITAFDARETAPEHVDKQFYLDDSGEYK
                     SFFDMTTHGKTVAVPAIPKLFDYIHKRYAKLSLEDLINPAIELAIEGHAANWATEKYS
                     RQQHARLTKYHETAQVFTHENQYWREGDWIVQPELGKTFQILREQGFNAFYKGDIAKQ
                     LVNVVKACGGTIILEDLANYDIQIKAPISATFKDYDIYSMGPSSSGGITVIQILKLLE
                     HVDLPSMGPRSVDYLHHLIQAMHLAYSDRAQYLADDNFHEVPVQSLIDDDYLKARSTL
                     INSNKANIDIEHGVVSDCISHTDVEENHTETTHFCVIDKEGNIASFTTSIGMIYGSGI
                     TIPGYGVLLNTTMDGFDVVDGGINEIAPYKRPLSNMAPTIVMHHGKPILTVGAPGAIS
                     IIASVAQTLINVLVFGMDIQQAIDEPRIYSSHPNRIEWEPQFSQSTILALIARGHAME
                     HKPDAYIGDVHGLQVDLNTRDASGGADDTREGTVIGGDVLSIRKQPLPSPKIYDNDTH
                     RVYFNDMQLPLYAEQVRWMHDKYWVDESVIRIIFPEVSVHIEDLRSYEIAGKNYIDIA
                     WLARKKGYQVTLKDDSLYLTDETYHSVKANTNAYYRYDRDSITR"
     gene            complement(2293..4068)
                     /locus_tag="NBI57_001627"
     CDS             complement(2293..4068)
                     /locus_tag="NBI57_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HCD1822566.1"
                     /translation="MKKIISIAIIVLALVLSGCGVPTKSEVAQKSSKVEVKGERPTIH
                     FLGQASYENDMNIVKDQLENAGFNVKMNIQPDYGSYRTQRQAGNYDIQIDDWMTVFGD
                     PNYAMTALFSSTGSNSLLKDKHVDQLLNKASTQNEADVKQTYKQIEDEVVFDKGYMAP
                     LYGSKKNLVYDNKVLDKNSVGLPNSRALIWQQFDYNNSRERDTRPLVMTQQDGEIPTL
                     DPIRSIAPSVYSINMNMYTRLLLLDENDHLTTKGSLSRDYAVNKDNKAFYFLLRDDDY
                     FAKVVNGQARNTGERVSAEDVKFSLDRARDKKSVPNNNTYNMHKHINDIKILKDEDID
                     QLRKEKDKDDKSIYDKLIKAYNVKSLTTDGQKVNNKDGIYQIVKITTDQSMPREVNYL
                     THSSAGILSKKFVNQVNQEYPKGYGDSSTIPANSDGKNALYASGAYIMTQKNAYQATF
                     QRNPGFNETEKGSYGSPKIKNITLKFNGDPNNALSELRNHSIDMLADVNQKHFDLIKS
                     DKNLSIIRKNGRKSVFLMLNIKKGIFKTHPNLRQAVVNAIDQDQFIKFYRGDKFKIAS
                     PITPLVDTGNEQRQDLEKVEKAINQ"
     gene            complement(4085..5248)
                     /locus_tag="NBI57_001628"
     CDS             complement(4085..5248)
                     /locus_tag="NBI57_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCD1822567.1"
                     /translation="MVKLTTKIASLKLFASYAIATYILVMLTSALNLFKGYVADTFYI
                     AETLLIILTIILIIILTTEQTWKHHDLWRRIVEVLLLLMALTGNVFTLLMFVSIRRYQ
                     RTSQIHSYNGWESFIRKTTRHRIAIIGLLILVYMLTLSIVSQFTFDTTLATKNQFNAL
                     LHGPSLAYPFGTDDFGRDLFTRVVVGTKLTFSISIISVVIAVIFGVLLGTIAGYFNHI
                     DNLIMRILDVVFAIPSLLLAVAIIASFGASIPNLIIALSIGNIPSFARTMRASVLEIK
                     RMEYVDAARITGENTWNIIWRYILPNAIAPMIVRFSLNIGVVVLTTSSLSFLGLGVAP
                     DVAEWGNILRTGSNYLETHSNLAIVPGVCIMFVVLAFNFIGDAVRDALDPRIH"
     gene            complement(5254..6732)
                     /locus_tag="NBI57_001629"
     CDS             complement(5254..6732)
                     /locus_tag="NBI57_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCD1822568.1"
                     /translation="MNCTFRLLTSNKFDMMLCLPIHKCVNLFYTFVYHSNLLKIILQG
                     VIIIITYLESFKSLYHKAFYKFVLSVLSLPIFLYAVIKFFFSAKRKNFYANNSEISEI
                     ERALHQKYKYLSQQKSSTQIHKEALKIFKAQSSNTSSKNIEQAHFSTYFENLLFHKFI
                     MIKVILALPMFILLTIYLQPLVRYIFERIVMAVIVIIGVIVSVFTILYFSPLDAAYSI
                     LGQNATKAQIHQFNVLHHLNEPYFIQLWDTIKGVFTFDLGTTYKGNEVVTKAVGERIP
                     ITIIVAVLALIVALIIAIPIGIISAMKRNSWLDITLMIIALIGLSIPSFWQGLLFILA
                     FSLKLDILPPSYMPEHPISLILPVLVIGTSIAASITRMTRSSVLEVMRSDYVLTAYAK
                     GLSTTQVVIKHILKNAIIPIVTLVGLLVAELLGGSAVTEQVFNINGIGRYIVQKQLIP
                     DIPAVMGGVVYISIVISLANLIIDIFYALIDPKLRSEINERK"
     gene            6689..8281
                     /locus_tag="NBI57_001630"
     CDS             6689..8281
                     /locus_tag="NBI57_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCD1822569.1"
                     /translation="MSNLLEVNSLNVQFNYDETTVQAVKNVSFELRKKHILGIVGESG
                     SGKSITAKSILGLLPDYPDHTLTGEIIFNGQSLNNLSTSALQQIRGKDISMIFQDPLS
                     SLNPRLTIGKQITEVLFQHKRVSKSEAKSMTINILEKVGIKHATRQFDAYPHELSGGM
                     RQRVMIAMALILKPQILIADEPTTALDASTQNQLLQLMKSLYEYTETSIIFITHDLGA
                     VYQFCDDVIVMKDGSVVESGTVESIFKSPQHTYTKRLIDAIPDIHQTRPPRPLNNDIL
                     LKFDRVSVDYTSPSGSLYRAVNDINLAIRKGETLGIVGESGSGKSTLAKTVVGLKEVS
                     EGFIWYNELPLSLFKDDELKSLRQEIQMIFQDPFASINPRFKVIDVIKRPLIIHGKVK
                     DNDDIIKTVVSLLEKVGLDQSFLYRYPHELSGGQRQRVSIARALAVEPKVIVCDEAVS
                     ALDVSIQKDIIELLKQLQLDFGITYLFITHDMGVINEICDRVAVMKNGEIVELNNTED
                     IIKHPQSDYAKQLISEVAVIAK"
     gene            complement(8791..8988)
                     /locus_tag="NBI57_001631"
     CDS             complement(8791..8988)
                     /locus_tag="NBI57_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000794565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCD1822570.1"
                     /translation="MKNNLKIARVSLSMTQKELANKVGVTRQTISLIEKGVHNPSLSL
                     CKNICSVLNKNLDEIFGEKPQ"
BASE COUNT         2883 a         1698 c         1404 g         3016 t
ORIGIN      
        1 cattataaaa acgcctcaca tcattcattt aattgtaccg aatcacatca agttagattt
       61 tagactaact attgtggtgt acatcaaaaa cgaatttgtg gggcgtttta tattttacaa
      121 aggcgactag cgtttcttat aacaacttca acattactac actcaagcat cactctaaag
      181 tttgaaatcg aaccctttcg ttcaacaaaa atcaaaatcc acttcaaact atatatcaaa
      241 tacataaatt atcttgtgat actatctcga tcatatctat agtatgcatt tgtgtttgct
      301 ttcactgaat gatatgtttc atcagttaag tataaactgt catcctttaa ggtaacttga
      361 taacctttct tccgtgccaa ccaggcaata tctatataat tttttccggc gatttcataa
      421 cttcttaaat cttcaatatg cacactaact tcagggaaaa tgattctaat aacactttca
      481 tcaacccaat atttgtcatg catccaccgc acttgttcag catataatgg caactgcata
      541 tcattgaaat atactcgatg ggtgtcatta tcgtatattt tcggactagg taatggttgt
      601 tttctaattg ataatacatc gccacctatc actgtgcctt ccctcgtatc atcagcacct
      661 cccgacgcgt cacgtgtatt caagtcaacc tgtaacccat gtacatctcc aatataggca
      721 tctggtttat gttccattgc atgtccacgt gcaatcaatg ctaatattgt agattgtgaa
      781 aattgaggct cccattcaat gcgattagga tggctactat aaattctagg ttcatctata
      841 gcctgctgaa tatccatgcc aaacactaat acattgatta atgtttgcgc aacactagca
      901 atgatactta tggcaccagg tgcacctact gttaatatcg gcttcccgtg atgcatcaca
      961 atcgttggag ccatgttact tagtggtcgt ttatatggtg caatttcgtt aataccacca
     1021 tctactacat caaagccatc cattgtcgta ttcaataaca caccgtagcc tggaatcgtg
     1081 atacctgaac cataaatcat accaattgat gtcgtaaatg aagcaatatt accttcctta
     1141 tcaatcacac aaaaatgagt tgtttcggta tgattttctt caacatctgt atgactaatg
     1201 caatcagaca caacaccatg ctctatatca atatttgctt tattgctatt aatgagcgta
     1261 ctgcgtgctt ttaaataatc gtcatcaatt aatgactgta caggcacctc atgaaaatta
     1321 tcatccgcca agtattgcgc gcgatcacta tatgctaaat gcatcgcttg tatcaaatga
     1381 tgcaagtaat cgacagatct tggacccata gatggtaaat cgacatgttc taataacttc
     1441 aatatttgaa tgaccgtgat accgccagaa ctagatggtc ccattgaata aatgtcatag
     1501 tctttaaatg tcgcactgat tggcgcttta atctgaatgt cataattggc tagatcctct
     1561 aaaatgattg tcccaccaca tgctttaaca acattgacta attgtttcgc aatgtcacct
     1621 ttataaaatg cattaaaccc ttgttctctt aatatttgaa atgtcttacc taattcgggt
     1681 tgtacaatcc aatcaccttc acgccaatat tgattttcat gcgtaaatac ttgtgccgtt
     1741 tcatgatact ttgtcaatcg tgcgtgttgc tggcgcgaat atttttcagt agcccaattg
     1801 gctgcatgac cttcaatggc tagttcaatt gcaggattaa ttaaatcttc caatgacaat
     1861 ttagcataac gcttgtgaat ataatcaaac agctttggaa ttgctggcac agcgacagtt
     1921 ttaccatgtg tagtcatatc aaaaaatgat ttatattcgc ctgaatcatc tagataaaat
     1981 tgtttgtcta catgttcagg tgctgtctca cgtgcatcaa acgcagttat actgccagta
     2041 ctttgctcat aatatagcaa gtacccgcca ccaccaatac ctgatgcaaa tggttctacc
     2101 acattcaatg ccagttgaat tgcaatcacg gcatccatgg cgttgccacc ttgatctaat
     2161 acatccttac caattttagc cgcaagagga tgtgatacgg aaattaaccc ttctttagat
     2221 gtttttgtct gtttgtcatt taagttaatg accatactat atcctcctac tttctgttaa
     2281 atatttaaaa cattattgat taatggcttt ttctactttt tctaaatctt gacgttgctc
     2341 gttaccagta tcgacaagtg gtgtaatcgg tgatgcaatt ttaaatttat cgccacgata
     2401 aaacttaata aattgatcct gatctatcgc attaactact gcttgtctca agtttggatg
     2461 cgtcttaaat ataccttttt taatatttag cattaaaaag actgacttgc gtccattttt
     2521 gcgaataatg cttaaatttt tatccgactt aattaaatca aaatgttttt gattcacatc
     2581 tgccaacata tcaattgaat gatttctaag ttctgacaat gcattattcg gatcaccatt
     2641 aaacttcaat gtaatatttt taattttagg tgatccataa ctaccttttt ctgtttcgtt
     2701 gaatcctgga ttacgttgaa acgttgcttg atatgcattt ttctgtgtca taatgtatgc
     2761 gccacttgca taaagcgcat tttttccatc tgaatttgca ggaattgtac tgctatcccc
     2821 atatcctttt ggatattctt gatttacttg attaacaaat tttttagata aaatgcctgc
     2881 cgaagagtgt gttaagtaat ttacctctcg aggcatcgat tgatctgtcg taattttaac
     2941 aatttgataa ataccgtctt tattatttac tttttgacca tctgtcgtta acgatttgac
     3001 gttataagct ttaatcaact tatcatagat tgatttatca tccttgtctt tctctttacg
     3061 caactgatcg atgtcctcat cttttaatat cttgatgtca tttatatgtt tgtgcatatt
     3121 gtaagtatta ttgttaggca cagacttttt atcacgtgct ctatctaaag aaaacttaac
     3181 atcttcagcc gatacacgct ctccagtatt acgtgcttgt ccattgacca ctttcgcaaa
     3241 ataatcatca tctcttaaca agaaataaaa tgctttattg tccttattca cagcataatc
     3301 acgacttaac gaacctttcg ttgttaagtg atcattttca tctaataata ataaccttgt
     3361 gtacatattc atattaattg aatatactga cggcgcaatt gaacgtattg gatccaatgt
     3421 aggaatttca ccatcttgtt gtgtcatcac aagtggccgc gtatctcgtt ctctactatt
     3481 gttgtaatca aattgttgcc atattaatgc acgtgaattt ggcaatccaa cactattttt
     3541 atctaacact ttattgtcat atactaaatt cttttttgat ccatataaag gcgccatata
     3601 ccctttatca aatacaactt catcttcaat ttgcttatat gtttgtttaa catctgcttc
     3661 attttgagta gaagctttat ttaacaactg gtctacatgt ttatctttca ataaactatt
     3721 tgatcctgta gaactaaata atgccgtcat agcatagttc gggtcaccaa acactgtcat
     3781 ccagtcatca atttggatat cataattgcc ggcttgacgt tgtgtacgat agctaccata
     3841 atctggttgg atattcatct tcacgttaaa tcctgcattt tccaattgat ctttaacgat
     3901 attcatatca ttttcataac ttgcttgtcc taggaaatgt attgttggtc gctcgccttt
     3961 cacttcaact ttcgatgact tttgagccac ttctgatttc gtagggacac cacaaccact
     4021 taataccaac gctaaaacta taattgcgat actaatgatt ttcttcacat ctatccctac
     4081 ctttttaatg aattcttgga tctagtgcat cacgcactgc atcacctata aaattaaatg
     4141 ctaaaacgac gaacataata caaacaccag gtacaatagc taaattactg tgcgtttcca
     4201 agtagttact accggtacgt aaaatgttgc cccattcagc tacatcaggt gcaacaccaa
     4261 gtcctaggaa acttaaacta cttgttgtta atacaaccac acctatattt aatgaaaaac
     4321 gtacaatcat aggcgcaatc gcattcggta aaatataacg ccatatgata ttccaagtgt
     4381 tttcaccagt gatacgtgct gcatctacat attccatgcg tttaatttct aaaacactgg
     4441 cacgcattgt ccgtgcaaat gatggtatat taccgatact taaagcaata attaaatttg
     4501 gaatacttgc tccaaatgat gcaataattg ccaccgctaa caataatgat ggaattgcaa
     4561 acactacatc taaaattcgc attattaaat tatcaatatg attaaaataa cctgcgatag
     4621 tgcctagtaa cacaccaaaa ataactgcaa taactactga aataattgaa attgaaaatg
     4681 tcagcttcgt tcctacaact acgcgtgtaa ataagtctct accgaaatca tcagtaccaa
     4741 acggataggc tagactcggt ccatgtaaca gtgcattgaa ctgattttta gtagccaatg
     4801 tcgtatcaaa tgtaaattgt gacacaattg ataatgtcag catgtagact aaaataagta
     4861 acccgataat cgcaatacga tgtctagtag tttttcgtat aaacgattcc cacccgttat
     4921 aactatgtat ttgcgatgta cgttggtaac gtctaatact tacaaacatt aataatgtaa
     4981 atacgttgcc tgttaatgcc atcaacaata acaacacttc gacgatacgt cgccataggt
     5041 catgatgctt ccatgtttgt tccgttgtta aaatgataat taaaatgatg gttaaaatga
     5101 ttagcaatgt ttcagcaata tagaacgtat cggccacata acctttaaaa agatttaatg
     5161 cactcgttaa cataactaaa atataagttg ctatagcata acttgcgaat agttttaagg
     5221 aagctatctt tgttgtaagt tttaccatat gcctcacttc ctttcgttaa tttcactacg
     5281 taattttgga tcgattaaag cataaaatat atcaataatt aagtttgcta aagatattac
     5341 aattgatata tatacgaccc cacccatgac tgctgggata tcaggtatta gttgtttttg
     5401 gacgatataa cgaccgatac cattaatgtt aaatacttgt tccgtcactg ctgaaccgcc
     5461 tagtaactct gccactagaa gaccaactaa cgttacaatt ggaataatgg catttttcaa
     5521 aatatgttta ataacaactt gtgtcgtcga taatcctttt gcataagcag ttaaaacata
     5581 atcgctgcgc attacttcaa gtacagaaga ccttgtcata cgcgtgatag aagcagcaat
     5641 acttgttcca atgacaagta caggtaaaat caacgatatt ggatgttctg gcatataaga
     5701 tggtggcaaa atatccaatt tcaatgagaa cgctaaaatg aataatagcc cttgccagaa
     5761 acttggaata gataaaccaa ttaatgcaat tatcattaac gtgatatcaa gccaactatt
     5821 tcgcttcatc gcactgataa taccaattgg tattgcaata attaatgcca caattagcgc
     5881 taatactgcg acaattattg taattggaat tctttcgcca actgctttag tcacaacctc
     5941 attccctttg taagtcgtac ctaagtcaaa ggtaaaaaca cccttgatgg tatcccacaa
     6001 ttgaataaaa taaggttcgt taagatgatg taatacattg aattgatgta tctgtgcctt
     6061 tgttgcattt tgtcccagta tgctataagc cgcatcaagc ggtgaaaaat acagaatggt
     6121 aaacacactg acaataacac caatgatgac aatcacagcc atgacaattc gctcaaaaat
     6181 atatctaact aatggctgta aataaatagt caataagatg aacatcggca aggccaatat
     6241 tactttgatc atgatgaact tatgaaataa taaattttca aagtatgttg aaaaatgtgc
     6301 ttgttcaata ttctttgaac tcgtattaga actttgtgcc ttgaatattt ttaatgcttc
     6361 tttatgtatt tgtgtggatg acttttgctg cgataaatat ttatattttt gatgtaacgc
     6421 tcgttcaatt tctgaaattt cagaattatt agcgtaaaaa ttttttctct tagcagaaaa
     6481 gaaaaacttt atcactgcat ataaaaatat tggcaagctt aataccgata atacaaactt
     6541 gtaaaatgcc ttgtgataca acgacttaaa agattctaaa taagtaatta tgataatcac
     6601 tccttgtaaa ataatcttga gtagattact atgatataca aaagtataga ataaatttac
     6661 acatttgtga atagggaggc acaacatcat gtcaaattta ttagaagtca acagtctgaa
     6721 tgtacaattc aattatgatg aaactacagt tcaagcggta aaaaatgtct ctttcgaatt
     6781 acgaaaaaaa catatcctag gtattgttgg tgaatcagga tcaggaaaaa gtattactgc
     6841 taaatctatt ttagggctac taccagatta tccagatcac acattaacag gagaaattat
     6901 ttttaatggg caatcgttaa ataatttatc aacttcagcg ttacaacaaa ttcgaggtaa
     6961 ggatatttca atgatttttc aagatccact ctcttcgttg aatccaagat taacgattgg
     7021 caaacaaatt acagaagtac tatttcaaca taaacgtgta tctaaatctg aagcaaagtc
     7081 gatgacaata aacattttag aaaaagtagg tataaaacat gcaactcgac aatttgatgc
     7141 ttatccacat gaactttctg gtggtatgcg tcaacgtgtc atgatagcaa tggcattgat
     7201 tttaaagcca caaattttaa tcgcagatga accaacaacg gcattagatg ccagtacaca
     7261 aaatcaatta ctgcagttaa tgaagtccct ttatgagtac acagaaacat ctattatttt
     7321 tatcactcac gatttaggcg ctgtgtatca attttgcgac gatgtgattg taatgaaaga
     7381 tggaagtgtc gttgaaagtg gcacggttga aagtattttt aaatcgccac aacataccta
     7441 tacaaaacgc ttaatagatg cgattcctga tattcatcaa acgcgtccgc caagaccgtt
     7501 aaacaatgat attttattaa aattcgatcg cgtgagcgtg gattacacat caccgagtgg
     7561 cagcctatac cgagcagtta atgatattaa cttggctatt agaaaaggcg aaacattagg
     7621 cattgtcggt gaatcagggt cagggaaatc gacattagct aagacggtcg tcggtctaaa
     7681 ggaagtgtca gaaggcttta tttggtataa cgaattacca ttaagtttat ttaaagatga
     7741 tgaattgaaa tctttacgac aagagataca aatgattttt caagatccat tcgcatctat
     7801 taatccaaga tttaaagtca ttgatgtgat taaacgacca ctaatcattc atgggaaagt
     7861 caaagataat gatgacatta ttaaaactgt cgtatcgttg ttagaaaagg ttggcctaga
     7921 tcaaagtttc ttatatcgct atccacacga attatctggt gggcaacgtc agcgtgtaag
     7981 tatcgcgaga gcacttgctg tagaacctaa agtgattgtt tgcgacgagg cagtgtccgc
     8041 tttagacgtt tcaattcaaa aagatatcat cgagttatta aaacaattac agttagactt
     8101 cggcatcact tatttattca tcacacatga catgggtgtt atcaatgaaa tatgtgatcg
     8161 cgttgcagtt atgaaaaatg gcgaaatcgt tgaactgaat aacacagaag atattatcaa
     8221 acatccgcaa tcagactatg caaagcaact tatttcagaa gtagcagtta ttgctaaata
     8281 aaagtcatgc gttgtgtaac tttatcactg tatggtctgg aataaatcgg gcaacctctg
     8341 atgtgttatg ttagatgact ttgtcatact aactatattg tgctaagtta gggctcggca
     8401 ccatatcaag ttttaagtgc cttgccatga tttaagggtc acacccatac tctgggcatt
     8461 ttgacgccag aataaatccc ccgccactat gtgaagtgcg ggggattatt tatattttat
     8521 tagaatattc agattttcga gtgtgtcaac ttagcttagt caatgtatat ttaacatcaa
     8581 ttactctttt tctttcataa ttaacacatt caaataaatg acatttaata cttttctcga
     8641 ttaattttct tttttaattc tatttttgat ttaataatgc tatatttcgg tattttaaat
     8701 ttataatacg tgtgttttct tttcaaaata atagcagttt ctgagaagaa atatattgtc
     8761 gacttttaga ttatctttca ataagctctt ttattgtggt ttctctccaa atatctcatc
     8821 caaattttta ttcaacaccg aacaaatatt tttacatagt gatagagagg ggttatgtac
     8881 acccttttct ataagagata ttgtttgtct agttactcca actttatttg ctagctcctt
     8941 ttgagtcata gatagtgaaa ctctagctat tttcaaatta tttttcactt ttaattattc
     9001 c
//
LOCUS       DAHHSC010000093         8980 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.094, whole genome shotgun sequence.
ACCESSION   DAHHSC010000093 DAHHSC010000000
VERSION     DAHHSC010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 8980)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8980)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8980
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.094"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            <1..348
                     /locus_tag="NBI57_001632"
     CDS             <1..348
                     /locus_tag="NBI57_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HCD1822571.1"
                     /translation="FRNLEFTCLQECINTADIIATGGGIIESEEAFNFLKNQKNIIWL
                     DCNIDIIYSRINDDPHRPNANNKTIKQLNDLYCSRILRYNEIAFKKFDSHLLSISEIY
                     YELLNLIKASDQY"
     regulatory      335..432
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            507..1598
                     /gene="gcvT"
                     /locus_tag="NBI57_001633"
     CDS             507..1598
                     /gene="gcvT"
                     /locus_tag="NBI57_001633"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HCD1822572.1"
                     /translation="MSSDLKQTPLYQNYVDRGAKIVEFGGWAMPVQFSSIKEEHNAVR
                     YEIGLFDVSHMGEIEVTGKDASQFVQYLLSNDTDNLTTSKALYTALCNEEGGIIDDLV
                     IYKLADDNYLLVVNAANTEKDFNWILKHKEKFDVEVQNVSNQYGQLAIQGPKARDLIN
                     QLVDEDVTEMKMFEFKQGVKLFGANVILSQSGYTGEDGFEIYCNIDDTEKIWDGLLEY
                     NVMPCGLGARDTLRLEAGLPLHGQDLTESITPYEGGIAFASKPLIDADFIGKSVLKDQ
                     KENGAPRRTVGLELLEKGIARTGYEVMDLDGNIIGEVTSGTQSPSSGKSIALAMIKRD
                     EFEMGRELLVQVRKRQLKAKIVKKNQIDK"
     gene            1618..2964
                     /gene="gcvPA"
                     /locus_tag="NBI57_001634"
     CDS             1618..2964
                     /gene="gcvPA"
                     /locus_tag="NBI57_001634"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPA"
                     /protein_id="HCD1822573.1"
                     /translation="MSHRYIPLTEKDKQEMLQTIGAKSIGELFGDVPSDILLNRDLNI
                     AEGEAETTLLRRLNRIASKNITKETHTSFLGAGVYDHYAPSVVDAMISRSEFYTAYTP
                     YQPEISQGELQAIFEFQTLICELTDMDVANSSMYDGMTSFAEACILAFSQTKKNKIVV
                     SKGLHYQALQVLHTYAKTRKEFEVVEIDLDGTVTDLKKLEAAVDDETAAVAVQYPNFY
                     GSIEDLEKIQSFIEDKKALFIVYANPLALGLLTPPGSFGADIVVGDTQPFGIPAQFGG
                     PHCGYFATTKKLMRKVPGRLVGQTQDDEGNRGFVLTLQAREQHIRRDKATSNICSNQA
                     LNALASSIAMSALGKQGIYDIAVQNIEHANYAKQQFIKKGFEVLDGTSFNEFVVKFDK
                     PIQQVNEELVKYNIIGGFDLGVVSDDFKNHMLIAVTELRTKDEIDTFVEKAGELND"
     gene            2957..4429
                     /gene="gcvPB"
                     /locus_tag="NBI57_001635"
     CDS             2957..4429
                     /gene="gcvPB"
                     /locus_tag="NBI57_001635"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HCD1822574.1"
                     /translation="MTSKSSPLIFERSREGRYAYSLPKSDIKTNSVESLLDDKFIRKN
                     KAEFPEVAELDLVRHYTELSNKNFGVDNGFYPLGSCTMKYNPKINEKVARIPGFSESH
                     PLQDEDQVQGSLEIIYSLQEELKEITGMDEVTLQPAAGAHGEWTALMIFKAYHENNGE
                     GHRDEVIVPDSAHGTNPASASFAGFKSVTVKSNERGEVDIDDLKRVVNENTAAIMLTN
                     PNTLGIFEKNIMEIREIVHNAGGLLYYDGANLNAIMDKVRPGDMGFDAVHLNLHKTFT
                     GPHGGGGPGSGPVGVVKELASYLPKPMVIKDGDKFKYDNDIKNSIGRVKPFYGNFGIY
                     LRAYTYIRTMGATGLKEVSEAAVLNANYIKARLSEHFEIPYKQYCKHEFVLSGVRQKE
                     FGVRTLDMAKRLLDFGVHPPTIYFPLNVEEGMMIEPTETESKETLDYFIDTLISIAEE
                     AKNDPDKVLEAPHTTVIDRLDEATAARKPILKFENLKQEK"
     gene            complement(4794..5180)
                     /locus_tag="NBI57_001636"
     CDS             complement(4794..5180)
                     /locus_tag="NBI57_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HCD1822575.1"
                     /translation="MSASLYIAIILVIAIIAYMIVQQILNKRAVKELDQNEFHNGIRK
                     AQVIDVREKVDYDYGHINGSRNIPMTMFRQRFQGLRKDQPVYLCDANGIASYRAARIL
                     KKNGYTDIYMLKGGYKKWTGKIKSKK"
     gene            5338..6168
                     /locus_tag="NBI57_001637"
     CDS             5338..6168
                     /locus_tag="NBI57_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HCD1822576.1"
                     /translation="MTETWNFINTGSKDPYYNMAMDEALLNFVSRGEIDPVIRFYTWN
                     PATLSIGYFQRLQKEIDIDKVKEKGFGLVRRQTGGRGVLHDKELTYSVIVPESHPNMP
                     STVTEAYRVISQGLLEGFKNLGFDTYFAVPKTPEERQKLKQPRSSVCFDAPSWYELVV
                     EGRKIAGSAQTRQKGVILQHGSILQDIDIDELFDMFIYKNERLKLKMKEAFVEKAVAI
                     NDISDEHITISQMEEAFEKGFKKGLNIELKPLELTEAQLAEVEELTEKYRSDEWMFRK
                     "
     gene            complement(6232..6450)
                     /locus_tag="NBI57_001638"
     CDS             complement(6232..6450)
                     /locus_tag="NBI57_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1822577.1"
                     /translation="MRNIIFYLVLIIAAIGLVMNLDAFIFSIVRMLISFAVIAGIIYL
                     IYYFFILTEDQRKYRKAMRKYKRNQRRK"
     gene            complement(6464..7045)
                     /locus_tag="NBI57_001639"
     CDS             complement(6464..7045)
                     /locus_tag="NBI57_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1822578.1"
                     /translation="MKKLVSIVGATLLLAGCGSQNLAPLEEKTTDLREDNHQLKLDIQ
                     ELNQQISDSKSKIKGLEKDKENSKKTASNNTKIKLMNVTSTYYDKVAKALKSYNDIEK
                     DVSKNKGDKNVQSKLNQISNDIQSAHTSYKDAIDGLSLSDDDKKTSKNIDKLNSDLNH
                     AFDDIKNGYQNKDKKQLTKGQQALSKLNLNAKS"
     gene            7150..8211
                     /locus_tag="NBI57_001640"
     CDS             7150..8211
                     /locus_tag="NBI57_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HCD1822579.1"
                     /translation="MSRITQVHRILEQKHLDAIIILSDYNRRYLSGFTGTSGALIISK
                     DKQYLITDFRYIDQATKQAPNYEIINRKSTIIGEIKELLHQENFENIGFEGHHVSYDT
                     YLELNKSRISLISISNTVDKIRDVKDVDEIALIQKAANIVDETYEYILTVVKAGMTEK
                     ELKAILESKMLELGADGPSFDTIVASGHRGALPHGVASDKIIEKGDMITLDFGAYYNG
                     YCSDITRTFAIGEPDPKLKEIYQIVLESQMKAINEIRPGMTGAEADAISRNYLESKGY
                     GKEFGHSLGHGIGLEIHEGPMLARTIQDKLQVNNCVTVEPGVYIEGLGGIRIEDDILI
                     TENGCQVFTKCTKDLIVLT"
     gene            8237..8794
                     /gene="efp"
                     /locus_tag="NBI57_001641"
     CDS             8237..8794
                     /gene="efp"
                     /locus_tag="NBI57_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000626504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HCD1822580.1"
                     /translation="MISVNDFKTGLTISVDNAIWKVIDFQHVKPGKGSAFVRSKLRNL
                     RTGAIQEKTFRAGEKVEPAMIENRRMQYLYADGDNHVFMDNESFEQTELSSDYLKEEL
                     NYLKEGMEVQIQTYEGETIGVELPKTVELTVTETEPGIKGDTATGATKSATVETGYTL
                     NVPLFVNEGDVLIINTGDGSYISRG"
BASE COUNT         3191 a         1214 c         1657 g         2918 t
ORIGIN      
        1 ttcaggaatt tagagttcac atgtttgcaa gaatgtatta acactgcaga tataattgct
       61 actggtggtg gtattattga aagtgaagag gcatttaatt ttttgaaaaa tcaaaaaaac
      121 attatttggt tagattgtaa tattgatatt atatatagtc gaatcaatga tgacccacat
      181 cgacctaatg caaataataa gacaatcaag cagttaaatg acttgtattg ctcgcggatt
      241 ttaagatata atgaaatcgc attcaagaaa tttgatagtc atttgctatc aatttcagaa
      301 atatattatg aattgctaaa tttaataaaa gcgagtgatc agtattagag agaatagagc
      361 gttaagactc tatcgccgaa ggtgcaagta atttattacg aaactctcag gcaaaaggat
      421 aatactgtaa cgcgttcctg aattggtgat ttataaacag ggtagcgatt gctatcctgt
      481 ttttataatt ttaagggggt atttcaatgt caagtgattt aaaacaaaca cctttatatc
      541 aaaattatgt tgatagaggt gcaaaaattg tggaattcgg aggatgggcg atgcctgttc
      601 aattttcaag tattaaagag gagcataatg ctgttcgata cgaaattggc ctgtttgatg
      661 ttagtcatat gggtgaaatt gaagtaacag gtaaagatgc tagtcagttt gtgcaatatt
      721 tattatcaaa tgatactgat aatttaacta cttcaaaagc attatatact gctttatgta
      781 atgaagaagg cggtattatt gatgatttag taatatataa attagctgac gacaattatt
      841 tattagttgt taatgctgct aatactgaaa aagattttaa ttggatttta aaacacaaag
      901 agaaatttga tgttgaagta caaaatgtat caaaccaata tggtcaatta gcaatacaag
      961 gaccaaaagc tagagattta attaatcaat tagttgatga agatgtaact gaaatgaaaa
     1021 tgtttgaatt taaacagggt gtcaaattat ttggagcaaa cgtcatttta tctcagtcag
     1081 gttacacagg tgaagatggg tttgaaattt attgtaatat cgatgatact gaaaaaattt
     1141 gggatggttt attagagtat aatgttatgc catgtgggtt aggcgctcgt gatacattaa
     1201 gattagaggc tggattgcca ttacatggac aagatttaac tgaatcaatt acaccatatg
     1261 aaggtggtat cgcttttgca agtaaaccat taattgatgc tgattttatt ggtaaatctg
     1321 tattaaaaga tcaaaaagaa aatggtgcac caagaagaac agtgggatta gaattacttg
     1381 aaaaaggaat tgcaagaact ggttatgaag ttatggattt agatggaaat attattggag
     1441 aagtaacttc aggaacacag tctccatcat caggaaaatc aattgcactt gcaatgataa
     1501 aaagagatga gtttgaaatg ggtagagagt tgcttgttca agttcgtaag cgtcaattaa
     1561 aagcgaaaat tgttaagaaa aatcaaattg ataaataatt aaaaaggggt gtgcattgtg
     1621 agtcatcgtt atataccttt aactgaaaaa gacaagcaag aaatgttaca aacaattggt
     1681 gcaaaatcta taggagaatt attcggtgat gtaccaagtg acattttatt aaatagagat
     1741 ttaaatattg ctgaaggcga agcggaaaca acgttactta gaagattaaa tcgcattgca
     1801 agcaagaata tcactaaaga aacgcataca tcatttttgg gtgctggtgt ttatgatcat
     1861 tacgcgccat cagttgtgga tgctatgatt tcaagatcag aattttacac agcatatacg
     1921 ccatatcaac cggaaatttc tcaaggtgaa ttacaagcca tttttgagtt ccaaacttta
     1981 atttgtgaat taacagatat ggatgtagca aattcttcta tgtatgatgg gatgactagc
     2041 tttgctgaag catgtatctt agcatttagt caaacaaaga aaaataaaat tgtagtctca
     2101 aaaggtttac attatcaagc attacaagta cttcatactt atgctaagac gcgtaaggaa
     2161 ttcgaagtag tggaaatcga tttagatggc actgttacag atttgaaaaa attagaagca
     2221 gcagtggatg atgaaacagc agctgttgca gtacaatatc caaactttta cggttcaatt
     2281 gaagatcttg aaaagattca aagctttatt gaagataaaa aagcattatt tatcgtatat
     2341 gcaaatccat tagcattggg attacttaca cctccaggtt catttggtgc agatattgtt
     2401 gtaggtgata ctcagccatt cggaattcct gctcaattcg gtgggccaca ttgtggatat
     2461 tttgcaacga cgaaaaagtt aatgcgtaaa gtgccaggta gattagtcgg tcaaacacaa
     2521 gatgatgaag gtaatcgtgg ctttgtatta acattacaag cacgtgaaca acatattcga
     2581 cgcgataaag cgacatctaa tatttgttct aaccaagcat taaatgcttt agcgtcatca
     2641 attgcaatgt cagcacttgg taaacaaggt atctatgata ttgcagttca aaatatagaa
     2701 catgctaact atgctaaaca acaatttatt aaaaaaggtt ttgaagtatt agatggtaca
     2761 tctttcaatg agtttgtagt caaatttgac aaaccaattc aacaagttaa cgaggaatta
     2821 gtgaagtaca atattatcgg tggttttgat ttaggtgttg tttcagacga ctttaaaaat
     2881 cacatgttaa tagctgtaac tgaattaaga acaaaagatg aaatcgatac atttgtagaa
     2941 aaggctggtg agttaaatga ctagtaaatc aagtccatta atttttgaaa gatctcgtga
     3001 aggcagatat gcatattcat taccaaaaag tgatattaaa acaaattctg ttgagtcatt
     3061 gttagatgat aaatttattc gtaaaaacaa agcggagttt cctgaagttg ctgaactaga
     3121 cttagtacgt cattatactg aactttcaaa taaaaatttc ggtgtagaca acggatttta
     3181 tccattgggt tcatgtacga tgaagtataa tcctaaaatt aatgaaaagg ttgctagaat
     3241 accaggattc agtgaatcac atccattaca agatgaagac caagttcaag gttcattaga
     3301 aattatttat agtttgcaag aagaattaaa agaaattact ggtatggatg aggtaacatt
     3361 acaaccagct gctggtgcac atggtgaatg gactgcattg atgatattta aagcttatca
     3421 tgagaataat ggtgaaggtc atcgtgatga agtcattgtg ccagattctg cgcatggtac
     3481 gaatccagcc tcagcttcat ttgcaggatt taaatcagtt actgtaaaat caaacgaacg
     3541 tggcgaagtt gatattgatg acttgaaacg tgttgtaaat gaaaatacag cagctattat
     3601 gttaactaat ccaaatactt taggtatttt cgaaaaaaac attatggaaa tccgtgaaat
     3661 cgtccataat gctggtggtc tattatatta tgatggtgcg aatttaaacg ctattatgga
     3721 caaagttcgc ccaggagata tgggatttga tgctgttcat ttaaacttgc ataaaacatt
     3781 tactggtcca catggtggcg gcggtcctgg ttcaggtcca gtcggtgtag taaaagaact
     3841 agcaagttac ttaccaaagc caatggttat taaagatggc gacaaattta aatatgataa
     3901 tgacattaaa aattctatcg gacgtgtaaa accattttat ggtaactttg gtatttactt
     3961 aagagcttat acgtatattc gaactatggg agcaactgga cttaaagagg tttcggaagc
     4021 agcggttctt aatgcgaatt atattaaagc acgtttatct gaacactttg aaatacctta
     4081 taaacaatat tgtaaacatg agtttgtgtt aagtggtgtg cgtcaaaaag aatttggtgt
     4141 acgtacttta gacatggcta agcgattatt agatttcggt gtacatccac caacaatata
     4201 cttcccatta aatgttgaag aaggtatgat gattgaaccg actgagacag agtctaaaga
     4261 aacacttgat tattttatcg atacattaat tagtattgct gaagaagcta aaaatgatcc
     4321 tgataaagtg ctagaagcac cacatacaac tgtgattgat cgattagacg aagctacagc
     4381 tgctcgtaaa ccaatattaa agtttgaaaa tcttaaacag gaaaaataaa gtattaaaca
     4441 catattccga gaattattat tctaactttg tatgaagatt taaggataat ggtttcaaaa
     4501 tcaattgaaa aagacaattt ctatttgaac aagaaaaata aaccgaagta ataactctta
     4561 gggtttggta ttattctttc atagaaattg tcttttcatt ttttagattg cggtaattga
     4621 atcgtattga aaatgagctg aactttcttt attatgctga aactaagttt aatgatcagt
     4681 gaagctagag aaatatagta acaggctttg ttaaaaaatt aaaatctgag tattgttatt
     4741 gatgatcaat taacactcag attttattaa atcgtatatt aaatttacaa aaactatttt
     4801 ttagacttta tttttccagt ccatttttta tagccgcctt ttaacatata gatatctgta
     4861 tatccattct ttttcaaaat acgagcggct ctatagctag caatcccatt ggcatcacat
     4921 aagtataccg gttgatcttt tcttaatcct tggaatcgtt gcctgaacat tgtcatagga
     4981 atattgcgag acccattaat gtgaccgtag tcatagtcaa ctttctctct aacatcgatg
     5041 acttgagctt ttctaatccc attatggaat tcattttgat ctaattcttt aacagctcgc
     5101 ttgttaagaa tttgttgaac aatcatataa gcaataattg ctataactaa aattattgcg
     5161 atgtacaaac tagcactcat cttgcatcct ccctaattaa acgataatat aattataaga
     5221 ctgttaggtc aaattatcaa aatatttcac taaagaattg caattattat tcatttagtt
     5281 aaattgatta attagcgaat caaatattat acattttgct gataggagtt cttgaatatg
     5341 actgaaactt ggaattttat taatacagga agtaaagacc catattataa tatggcaatg
     5401 gatgaagcgt tattaaattt tgtctctaga ggtgaaattg atccagttat acgtttttac
     5461 acatggaatc ctgcaacatt atcaataggg tatttccaaa ggctacaaaa agaaattgat
     5521 attgataagg ttaaagagaa aggttttggt ctcgtaaggc gtcaaactgg tggccgtggg
     5581 gtgctacatg ataaagagtt aacttacagt gtgattgtgc ctgaatcgca tccgaatatg
     5641 ccttctacag ttacagaagc gtacagagtg atttcacaag gattattaga aggatttaaa
     5701 aacttaggat ttgatactta ttttgcggta cctaaaacgc ctgaagagcg tcaaaaatta
     5761 aaacaacctc gaagttcagt gtgttttgat gcaccaagtt ggtatgagct agttgttgaa
     5821 ggccgtaaga ttgctgggag tgctcaaacg agacaaaagg gcgtaatcct ccaacacggt
     5881 tcgattttac aagatattga tattgatgaa ctgtttgata tgtttattta taaaaatgaa
     5941 agattaaaat taaaaatgaa agaggcattc gttgaaaaag cagttgccat caatgatatt
     6001 tcagatgaac acattactat tagtcaaatg gaagaagctt tcgagaaagg ttttaagaaa
     6061 ggtttaaata tagaattaaa gccacttgaa ttaacagaag ctcaattggc agaagtggaa
     6121 gaattaactg aaaaatatcg ttcagatgaa tggatgttta gaaagtagtt tttagaataa
     6181 aaaatggaag cacgtagact attttaccta caagtgcttc cgttttttta tctattttct
     6241 tctttgattt cttttatact tacgcattgc tttgcgatat ttgcgttggt cttcagttaa
     6301 gatgaagaaa taataaatca gataaataat accagctatt acagcaaagc tgattaacat
     6361 tctgacgatt gaaaaaataa aggcatctag attcattact aatccaatcg cagcaataat
     6421 aagtacaaga taaaatatta tattacgcat taaaagactc ctatcatgat tttgcattta
     6481 agtttaattt tgacaacgct tgttgtcctt ttgtaagttg ttttttatct ttattttgat
     6541 agccattttt aatatcatca aatgcatgat tcaaatcaga gtttaattta tcgatatttt
     6601 tagacgtttt tttatcatca tcactaagtg ataaaccatc gatagcatct ttgtatgaag
     6661 tgtgagcact ttgaatatca ttagaaattt gatttaattt cgattgaaca ttcttatcgc
     6721 ctttgttttt acttacatct ttctcaatat cgttatagga tttcaaagct ttagcaactt
     6781 tgtcgtagta tgttgatgta acattcatca atttaatttt cgtattatta gatgcagttt
     6841 ttttactgtt ttctttatcc ttttcaagcc ctttaatttt agatttagaa tcactaattt
     6901 gttgattaag ttcttgaata tctagtttga gttgatgatt atcttctctt aaatctgttg
     6961 ttttttcttc taatggtgct aaattttgtg atccacatcc agctaacaat aatgttgcgc
     7021 caacaattga aaccaatttt ttcattacta tctccctaaa atcaatatct attatttttt
     7081 atccttatta tgatattatt ttaatgtata aagttgaaat gcacaaacgt ttcactagga
     7141 ggtaataaga tgagcagaat aacacaagtg catcgtattt tagaacaaaa acatttagat
     7201 gcgattatca ttctttcgga ttacaataga agatatttat caggttttac tggtaccagt
     7261 ggagcactta tcatatctaa agataaacaa tatttgataa cagactttag atatattgat
     7321 caagcaacta agcaggcgcc aaattatgaa attattaatc gtaaatctac tattattggt
     7381 gagattaaag aattgctaca ccaagaaaat tttgaaaata taggttttga ggggcatcat
     7441 gtaagttatg atacatacct tgaattaaat aaaagccgta tatcattaat aagcatttct
     7501 aatactgtag ataaaattag agacgtcaaa gatgttgacg aaattgcttt aattcaaaaa
     7561 gcagctaata ttgttgatga aacatatgaa tatattttaa ctgttgtaaa agcaggtatg
     7621 actgaaaaag aattaaaggc aatattagaa agtaaaatgc tagaattagg agcagatgga
     7681 ccatcatttg atacgattgt agcatctggt catagaggtg cattaccaca tggtgttgca
     7741 agtgataaaa ttattgaaaa aggcgacatg attacattag attttggcgc gtattataac
     7801 ggctattgtt cagatattac tagaacattt gctattggag aaccagatcc taaactgaaa
     7861 gaaatatatc aaatagtact tgaatctcaa atgaaagcaa ttaatgagat tagacctggc
     7921 atgactggtg cagaagctga tgccatttca agaaactatt tagagtcaaa agggtatgga
     7981 aaagaatttg gacattcact aggacatggt attggtttag aaatccatga agggccaatg
     8041 ctggctcgta cgatacaaga taaacttcaa gttaacaact gtgttacagt agaacctggt
     8101 gtttatatag aaggtttggg cggtataaga atagaagatg atattttaat tacagaaaat
     8161 ggttgtcaag tctttactaa atgcacaaaa gaccttatag ttttaacata agcgtgtaaa
     8221 atgaggagga aactgaatga tttcggttaa tgattttaaa acaggtttaa caatttctgt
     8281 tgataacgct atttggaaag ttatagactt ccaacatgta aagcctggta aaggttcagc
     8341 attcgttcgt tcaaaattac gtaatttaag aactggtgca attcaagaga aaacgtttag
     8401 agctggtgaa aaagttgaac cagcaatgat tgaaaatcgt cgcatgcaat atttatatgc
     8461 tgacggagat aatcatgtat ttatggataa tgaaagcttt gaacaaacag aactttcaag
     8521 tgattactta aaagaagaat tgaattactt aaaagaaggt atggaagtac aaattcaaac
     8581 atacgaaggt gaaactatcg gtgttgaatt acctaaaact gttgaattaa cagtaactga
     8641 aacagaacct ggtattaaag gtgatactgc aactggtgcc actaaatcgg caactgttga
     8701 aactggttat acattaaatg tacctttatt tgtaaacgaa ggtgacgttt taattatcaa
     8761 cactggtgat ggaagctaca tttcaagagg ataatctcta atttgttaac aaatagcttg
     8821 tattcactat actgatttaa cgtaagatat tctaaataag tctcataaag ctattgccta
     8881 aaatgattat aggttatatg ctgatatgag gctttttatt tttaaaataa tttttaaaat
     8941 ataaaacgaa tcgtctaatt ggtagtgaaa aaacatagac
//
LOCUS       DAHHSC010000094         8965 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.095, whole genome shotgun sequence.
ACCESSION   DAHHSC010000094 DAHHSC010000000
VERSION     DAHHSC010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 8965)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8965)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8965
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.095"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            complement(56..1036)
                     /locus_tag="NBI57_001642"
     CDS             complement(56..1036)
                     /locus_tag="NBI57_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HCD1822581.1"
                     /translation="MLKLNPYKIGFRTIKTAVGMTLGVIISKLLGLDNYASSAILVVL
                     CIKHTKVHSLQAIISRLVSCFLVLFLGSAIFSLLGQSPIVLGIIVLLFIPLTVVLKVQ
                     EGVITSCVILLHVFNAKSIDAHLIVNETLLLLIGLSIAFTMNLMMPSLDKQLDEYKCK
                     IEQQIADIFSKYSYICEKYEDTIAIEFEVLLLNIKKAKSIAFRDVKNHFVRNENSYYH
                     YFDMREEQVELLMRMKPLIESICHKDPLLNDVSSLLKEIGLNVNSNDYTAMRLHNLYE
                     TRLKLDELPLPTTHKALNSRASIIQILNELEEYLQIKSQFGSLKMHSEVA"
     gene            complement(1050..1487)
                     /locus_tag="NBI57_001643"
     CDS             complement(1050..1487)
                     /locus_tag="NBI57_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrxA/BrxB family bacilliredoxin"
                     /protein_id="HCD1822582.1"
                     /translation="MDMNFDLYMNGVVEQARNEIESAGYEQLTTAEDVDKVLKQDGTT
                     LVMINSVCGCAGGIARPAASHALHYDVLPDRLVTVFAGQDKEATQRAREYFEGYAPSS
                     PSFALVKDGKITEMIERHQIEGHDVMNVINQLQTLFNKYCEER"
     gene            complement(1838..3112)
                     /locus_tag="NBI57_001644"
     CDS             complement(1838..3112)
                     /locus_tag="NBI57_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HCD1822583.1"
                     /translation="MEITMPKLGESVHEGTIEQWLVSVGDHIDEYEPLCEVITDKVTA
                     EVPSTISGTITEILVEAGQTVAIDTIICKIETADEKTNETTEEIQAKVDEHTQKSTKK
                     ASATVEQTFTAKQNQPRNNGRFSPVVFKLASEHDIDLSQVVGSGFEGRVTKKDIMSVI
                     ENGGTTAQSDKQVQTKSTSVDTSSNQSSEDNSENSTIPVNGVRKAIAQNMVNSVTEIP
                     HAWMMIEVDATNLVNTRNHYKNSFKNKEGYNLTFFAFFVKAVADALKAYPLLNSSWQG
                     NEIVLHKDINISIAVADENKLYVPVIKHADEKSIKGIAREINTLATKARNKQLTTEDM
                     QGGTFTVNNTGTFGSVSSMGIINHPQAAILQVESIVKKPVVINDMIAIRSMVNLCISI
                     DHRILDGLQTGKFMNHIKQRIEQYTLENTNIY"
     gene            complement(3125..4108)
                     /locus_tag="NBI57_001645"
     CDS             complement(3125..4108)
                     /locus_tag="NBI57_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HCD1822584.1"
                     /translation="MAKLSYLEAIRQAQDLALQQNKDVFILGEDVGKKGGVFGTTQGL
                     QQQYGEDRVIDTPLAESNIVGTAIGAAMVGKRPIAEIQFADFILPATNQIISEAAKMR
                     YRSNNDWQCPLTIRAPFGGGVHGGLYHSQSIESIFASSPGLTIVIPSTPYDAKGLLLS
                     SIESNDPVLYFEHKKAYRFLKEEVPEEYYTVPLGKADVKREGEDLTVFCYGLMVNYCL
                     QAADILAADGINVEVVDLRTVYPLDKETIIDRAKNTGKVLLVTEDNLEGSIMSEVSAI
                     IAEHCLFDLDAPIMRLAAPDVPSMPFSPVLENEIMMNPEKILNKMRELAEF"
     gene            complement(4108..5100)
                     /locus_tag="NBI57_001646"
     CDS             complement(4108..5100)
                     /locus_tag="NBI57_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HCD1822585.1"
                     /translation="MIDYKSLGLSEEDLKVIYKWMDLGRKIDERLWLLNRAGKIPFVV
                     SGQGQEATQIGMAYALEEGDITAPYYRDLAFVTYMGISAYDTFLSAFGKKDDVNSGGK
                     QMPSHFSSRAKNILSQSSPVATQIPHAVGAALALKMDGKKKIATATVGEGSSNQGDFH
                     EGLNFAGVHKLPFVCVIINNKYAISVPDSLQYAAEKLSDRALGYGIHGEQVDGNDPLA
                     MYKAMKEARDRAISGQGSTLIEAVTSRMTAHSSDDDDQYRTKEEREALKKADCNEKFK
                     KELLSAGIIDDAWLAEIEAEHKDIINKATKAAEDAPYPSVEEAYAFVYEEGSLN"
     gene            complement(5116..6537)
                     /gene="lpdA"
                     /locus_tag="NBI57_001647"
     CDS             complement(5116..6537)
                     /gene="lpdA"
                     /locus_tag="NBI57_001647"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HCD1822586.1"
                     /translation="MSEKQYDLVVLGGGTAGYVAAIRASQLGKKVAIVERQLLGGTCL
                     HKGCIPTKSLLKSAEVFQTVKQAAMFGVDVKDANVNFENMLARKEDIINQMYQGVKHL
                     MQHNHIDIYNGTGRILGTSIFSPQSGTISVEYEDGESDLLPNQFVLIATGSSPAELPF
                     LSFDHDKILSSDDILSLKTLPSSIGIIGGGVIGMEFASLMIDLGVDVTVIEAGERILP
                     TESKQASQLLKKSLSARGVKFYEGIKLSENDINVNEDGVTFEISSDIIKVDKVLLSIG
                     RKPNTSDIGLNNTKIKLSTSGHILTNEFQQTEDKHIYAAGDCIGKLQLAHVGSKEGVV
                     AVDHMFEGNPIPVNYNMMPKCIYSQPEIASIGLNIEQAKAEGMKVKSFKVPFKAIGKA
                     VIDSHDANEGYSEMVIDQSTEEIVGINMIGPHVTELINEASLLQFMNGSALELGLTTH
                     AHPSISEVLMELGLKAESRAIHV"
     gene            complement(6688..8367)
                     /gene="recN"
                     /locus_tag="NBI57_001648"
     CDS             complement(6688..8367)
                     /gene="recN"
                     /locus_tag="NBI57_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HCD1822587.1"
                     /translation="MLQTLSIKQFAIIEELEIQFSDGLTVLSGETGSGKSIIIDAIGQ
                     LIGMRASSDFVRHGEKKAVIEGIFDIDESKDAIHILKNMDIDVDEDFLLVKREIFSSG
                     KSLCKINNQTVTLQDLRKVMQELLDIHGQHETQSLLKQKYHLTLLDNYAESRYQDLLD
                     KYHQTFQNYKAKKQELEDLESADQALLQRLDLMKFQLEELSEAHLKEGEIEQLEIDIK
                     RIQNSEKLSLALNNAHMTLTDENAITDRLYELSNHLLTINDIVPNKYDKLKEDIDQFY
                     YILEDAKHELYDEMANTEFDEQVLNEYESRMNLLNNLKRKYGKDISELIAYQEKLNNE
                     INKIENYEQSTSQLREEINALYNQVIEVGQALSKQRRIVARELRDHIVSEIQNLQMKD
                     ANLEISFKKLEEPNIDGIEFVEFLISPNKGEPLKSLNKIASGGELSRIMLALKSIFVK
                     SRGQTAILFDEVDSGVSGQAAQKMAEKMRDIAEYIQVICISHLPQVASMSDHHLLISK
                     SSKDDRTTTQVQELIGDDKVDEIARMISGASVTDLTRENAREMIQHNQRRR"
     gene            complement(8383..8835)
                     /gene="argR"
                     /locus_tag="NBI57_001649"
     CDS             complement(8383..8835)
                     /gene="argR"
                     /locus_tag="NBI57_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ArgR"
                     /protein_id="HCD1822588.1"
                     /translation="MPKKSVRHIKIREIISNEQIETQDELVKRLNDYDLNVTQATVSR
                     DIKELQLIKVPIPSGQYVYSLPNDRKFHPLEKLGRYLMDSFVNIDGTDNLLVLKTLPG
                     NAQSIGAILDQINWEEVLGTICGDDTCLIICRSKEASDEIKSRIFNLL"
BASE COUNT         2706 a         1727 c         1231 g         3301 t
ORIGIN      
        1 gttatcgtat tgcaacgatt actttcaaac atgggtcata cggatggatt attttttaag
       61 ctacttcact atgcattttc aatgaaccaa attgcgattt gatttgtaaa tattcttcta
      121 attcatttaa tatttgaata atacttgctc tcgagttaag cgctttgtgt gttgttggca
      181 atggcagttc atccaatttc aaacgcgtct catacaaatt gtgtaaacgc attgctgtat
      241 agtcattact attcacattt agaccaattt ctttcagcag tgacgaaaca tcatttaaaa
      301 gtggatcttt atgacagata ctttcgatga gcggtttcat tctcattaac aattccactt
      361 gctcttctcg catatcaaaa taatgatagt atgaattttc gtttctaaca aaatgatttt
      421 taacatctcg gaacgcgata gacttcgcct ttttaatatt taaaagtaac acttcaaatt
      481 caatcgcaat ggtatcttca tatttttcac aaatataact atatttacta aaaatatcag
      541 caatttgttg ctcaatttta catttgtatt cgtctagttg tttgtctaaa cttggcatca
      601 ttaaattcat tgtaaatgca atgctcagtc caattaacag taataatgtt tcattaacaa
      661 ttaaatgtgc atcaattgat tttgcattaa aaacatgaag taatataacg caactcgtaa
      721 tgacaccttc ttgtactttt aatacgacag ttaatggtat aaataacaat acgataatac
      781 cgagtacaat tggactctga cctaataaac taaatattgc tgaacctaaa aacaatacta
      841 aaaaacatga tactaatctt gaaataatcg cttgtagcga atgtactttt gtatgtttaa
      901 tacataatac gactaatatg gcgcttgaag cataattatc taaacctaac agcttactaa
      961 taattacacc taaagtcata cccactgctg tttttattgt tctaaatcca atcttgtaag
     1021 gatttaactt taacatgggt tagcgcctct tatctttctt cacaatattt attgaataat
     1081 gtttgtaatt gattaattac gttcatcaca tcatgacctt cgatttgatg tctttcaatc
     1141 atttctgtaa tctttccatc ttttactaat gcaaatgacg gacttgaagg cgcataacct
     1201 tcgaagtatt cacgcgctct ttgtgtcgct tctttatctt gtccagcaaa tactgtcact
     1261 agacgatcag gtaatacgtc ataatgtaaa gcatgtgatg ctgctggtct tgcgatacca
     1321 cctgcacaac cacatacaga attgatcata actagtgttg taccatcttg tttaagaact
     1381 ttgtcaacat cttctgcagt agttaattgc tcatatcccg cagattcaat ttcattcctt
     1441 gcttgttcta caacaccgtt catgtataaa tcgaaattca tgtccataag ttcaatcacc
     1501 tatcccttta tatttaaact atcctcattc tactaattaa taacatattg ttcaataaac
     1561 taatctgaat cacaccaata tttagacaca attttaacaa tataccaaac attattgtgc
     1621 ttaatatcat ggaaactaat ttgttcacct gttttcatta atatgtttcc agtatgatgt
     1681 attattttga ctttactgca aaaatgcatt caaccatgtt gattattgtt ctttatcttt
     1741 ttttgaattt attgcacata ttttattgcc caaaaaaata catccatcga caagaacaag
     1801 ataaaacaag ttgtcgatag atgtatccat gttatcacta atatatattt gtattttcta
     1861 aagtatactg ttcgatacgc tgtttaatat gattcataaa tttaccagtt tgtaaaccat
     1921 ctaaaatgcg atgatctatt gaaatacata aatttaccat acttcgaatt gcaatcatat
     1981 cattaattac tactggcttt ttaacgattg attctacttg taatatcgct gcttgagggt
     2041 gatttataat ccccattgat gatactgaac caaatgtacc agtattatta actgtaaatg
     2101 ttccaccttg catatcttca gttgtcaatt gcttattacg cgcttttgtt gctaaagtat
     2161 taatttctct agctatacct ttgattgact tttcgtctgc atgcttaatc acaggtacgt
     2221 ataatttatt ttcatcagca acagcaattg aaatattaat gtctttatgt aagacaattt
     2281 catttccttg ccagctacta tttaataaag gatatgcttt taaagcatct gctacagctt
     2341 ttacaaagaa agcaaagaac gttagattat atccttcttt atttttaaag ctgtttttat
     2401 aatgatttct cgtattcaca agatttgtag catctacttc aatcatcatc catgcatgtg
     2461 gaatctctgt tacactatta accatatttt gcgcaattgc tttacgcaca ccatttactg
     2521 gtattgtgct gttttcacta ttgtcttcag atgattggtt acttgatgta tctactgatg
     2581 ttgattttgt ttgaacttgt ttgtcagatt gagctgtggt accaccattt tcaataactg
     2641 acattatatc cttcttagtt acacgacctt caaatccact acctacaact tgtgataaat
     2701 caatgtcatg ctctgaagcg agtttaaata caacaggtga aaagcgacca ttattacgag
     2761 gttgattttg tttagcagta aatgtctgtt ccactgttgc actagctttt ttagtagatt
     2821 tctgagtatg ctcatccact tttgcttgta tctcttcagt tgtttcattt gtcttttcat
     2881 cagcagtttc aattttacag ataattgtat caatagctac tgtctgcccc gcttcaacta
     2941 aaatttctgt aattgttcct gatatcgtgg aagggacttc agctgtcact ttatctgtaa
     3001 taacttcaca taatggttca tattcatcaa tatgatcacc aacagaaact aaccattgtt
     3061 caatggtacc ttcatgaaca ctctcaccta acttaggcat tgttatttcc atgactttcc
     3121 ctccctagaa ttctgctaat tcacgcattt tatttaagat tttttctgga ttcatcataa
     3181 tttcattttc taatacagga gaaaatggca tagatggtac atctggagca gctaaacgca
     3241 tgattggtgc atctaaatcg aacaagcaat gctctgcaat aatcgctgac acttctgaca
     3301 taatactacc ttctaaatta tcttcagtta caagtaaaac tttacctgta tttttagcac
     3361 gatcaataat tgtttcttta tctaatggat aaacagttcg taaatcaacg acttcaacgt
     3421 tgataccgtc tgcagctaaa atatccgctg cttgtaaaca ataattgacc attaatccat
     3481 aacaaaatac tgttaaatct tcaccttcac gtttaacatc tgcttttcct aaaggtacag
     3541 tgtaatattc ttctggcact tcttccttta agaaacgata agctttttta tgctcaaagt
     3601 acaatactgg atcatttgat tcgatagatg ataataaaag ccctttagca tcatacggtg
     3661 tggaaggaat aacaattgtt aaacctggtg atgaagcaaa tatactttca atactttgtg
     3721 aatgatatag tcctccgtga acaccgccac caaatggtgc acgaatcgtt aatgggcatt
     3781 gccaatcatt atttgaacga taacgcattt tcgcagcttc actaataatt tgatttgtcg
     3841 caggtaaaat aaaatctgca aattgaattt ctgcaattgg tcttttacct accatagctg
     3901 caccaatggc agttccaaca atatttgact cagctaatgg cgtatcgata actctgtctt
     3961 caccatattg ttgttgtagt ccttgagtag taccaaatac gccacctttt ttaccaacat
     4021 cttcaccaag aataaacaca tctttatttt gttgtaatgc taagtcttgt gcctggcgta
     4081 tcgcctctaa ataagataat ttagccatta gttaagactc ccttcttcgt acacaaatgc
     4141 ataggcttct tcgacacttg gatatggcgc gtcttcagca gcctttgtcg ctttattgat
     4201 gatgtcttta tgctccgctt ctatttctgc caaccaagca tcatcgataa tgccagctga
     4261 aagcaactct tttttgaact tttcattgca gtcagctttt ttaagcgctt cacgctcttc
     4321 tttcgtacga tattggtcgt catcatctga tgaatgagct gtcatacgac ttgttactgc
     4381 ttcaatcaaa gttgaacctt gaccagaaat agctcgatct cttgcttctt tcatcgcttt
     4441 atacattgct aatggatcat taccatctac ttgttcacca tgtataccgt aaccaagtgc
     4501 tctatccgat aatttttcag ctgcgtattg taatgaatca ggtactgaaa ttgcatattt
     4561 attatttata atgacacata caaaaggaag tttgtgtaca cccgcgaagt ttaaaccttc
     4621 atggaagtca ccttggtttg agctaccttc accaacagtt gctgttgcaa ttttcttctt
     4681 accatccatt tttaaagcta aagcagcacc aacagcatgg ggtatttgag ttgctaccgg
     4741 tgaactttga gacaaaatat tcttagctct actactaaag tgtgatggca tttgttttcc
     4801 accagagtta acatcgtctt tctttccaaa cgctgataaa aacgtatcat acgctgagat
     4861 acccatataa gtaacgaaag ctagatctct ataataaggc gctgtaatat caccttcttc
     4921 taatgcgtat gccatcccaa tctgagttgc ttcttgtcct tgaccactta caacaaatgg
     4981 aattttacct gcacggttca ataaccacag tctttcatct atttttctac ctaaatccat
     5041 ccatttatat attactttta ggtcttcttc gctaaggcct aatgatttat aatcaatcat
     5101 gttaaatcct cctatttata cgtgaatagc tctactttct gctttcaatc ctaattccat
     5161 caacacttca gagatggaag gatgtgcgtg tgttgttagt cctaattcta atgccgagcc
     5221 attcatgaac tgtaacagtg atgcctcatt aatcaattct gttacatgtg gaccaatcat
     5281 attaataccc acaatttctt cagttgattg atcaatcacc atttcgctat acccttcgtt
     5341 tgcgtcatgg ctatcaatca ctgctttacc aattgcttta aatggtactt taaaactttt
     5401 aactttcatt ccctctgcct ttgcttgttc aatgtttaaa ccgatagaag caatttcagg
     5461 ttgtgaataa atacacttag gcatcatgtt atagtttact gggattgggt tcccctcaaa
     5521 catatgatca acagccacaa caccttcttt tgatccaaca tgtgccaatt gtaattttcc
     5581 tatacaatca ccagctgcat aaatatgttt atcttcagtt tgttgaaatt cgttcgttaa
     5641 aatatgtcct gatgtagaaa gttttatttt agtgttgttt aaaccaatat ctgatgtgtt
     5701 aggttttcta ccaatcgata gcaacacttt atctacttta attatgtctg aagaaatttc
     5761 aaacgtaaca ccatcttcgt taacatttat atcattttca gaaagtttta ttccctcata
     5821 gaatttaaca ccacgtgctg acaatgattt ttttaatagt tgtgaagctt gtttactttc
     5881 agttggtaaa attctttcac ctgcttctat aactgttacg tcaacaccta aatctatcat
     5941 caatgatgca aattccattc cgataacacc accaccaata ataccaatac ttgatggtaa
     6001 cgtctttaat gataatatat catcgctaga taaaatttta tcatgatcaa atgataagaa
     6061 tggcaactct gcaggcgaag aaccagttgc aattaataca aattggttgg gtaataagtc
     6121 tgattcacca tcttcatatt cgacagaaat tgtgccactt tgaggtgaaa atatagatgt
     6181 acctagaata cgtcccgtgc cattataaat gtcaatgtga ttgtgttgca ttaaatgctt
     6241 tacaccttga tacatttgat taataatgtc ttcttttcgt gccaacatat tttcaaaatt
     6301 aacattagca tctttgacat caacgccaaa cattgctgcc tgttttactg tttgaaatac
     6361 ttcagcagat ttaagcagcg atttagtagg aatacaacct ttatggagac aagtacctcc
     6421 taatagttgt cgttctacta ttgccacttt cttacctaat tgagacgcac gtatcgcagc
     6481 aacatatcct gcagtacctc caccgagaac gactaaatca tattgtttct ctgacatgtt
     6541 cttactccta actaatgata tatatccatt gaaaatttat taatacatag ttttcatgtc
     6601 cattaattac ctattttaca tgattgtcta tttagtttga atgcacataa ataaatccat
     6661 aaatgagtat tcaacacact ttggaaatta tctacgtctt tgattgtgtt gtatcatttc
     6721 tcttgcattt tctcgagtta aatcagtaac acttgcacct gaaatcattc gtgcaatttc
     6781 atctacttta tcatcgccaa ttaactcttg aacttgtgtt gttgtacgat catcttttga
     6841 tgacttcgaa attaataaat gatggtcgct catcgatgca acttgtggca agtgagaaat
     6901 acaaataact tgtatatatt ctgctatatc tcgcattttc tctgccattt tttgtgcggc
     6961 ttgtccagat acacctgagt caacctcatc gaataaaatt gcagtttgac ctctcgattt
     7021 aacaaaaata ctttttaacg ctaacataat tctagaaagt tctccacctg atgcaatttt
     7081 atttaaactt tttaatggtt cccctttatt tggactgatt aaaaattcta caaattcgat
     7141 tccatcaata ttcggttctt ctaatttttt aaatgaaatc tcaagatttg cgtctttcat
     7201 ttgtaagttt tgaatttccg atacaatgtg gtctcttaac tccctagcga ctatacgacg
     7261 ctgctttgat aacgcttgtc caacctctat aacttgatta tacaatgcat ttatttcttc
     7321 tcgtaactgc gatgtacttt gttcatagtt ttcaatttta ttgatttcat tattaagctt
     7381 ttcttgatat gcgattaatt ctgaaatatc ttttccatat ttacgtttta aattattcag
     7441 caaattcata cgagattcat actcgtttaa cacttgttcg tcgaattctg tattagccat
     7501 ttcatcatat aactcatgtt ttgcatcttc taaaatgtaa taaaattgat caatatcttc
     7561 ttttaatttg tcatatttat ttggaactat atcgtttatt gttaacaaat ggttgcttag
     7621 ttcatacaaa cgatcagtga tagcattttc atccgttaat gtcatatgtg cgttattaag
     7681 cgctaagctt aatttttcag agttttgaat gcgtttaata tctatttcaa gttgctctat
     7741 ttcgccttct tttagatgtg cttcagacaa ttcttctaat tggaatttca ttaaatctaa
     7801 acgctgcagc aatgcttggt ctgctgattc taaatcttct aactcttgct ttttggcttt
     7861 ataattttga aaagtttggt gatatttatc caacaaatct tgataacgtg attctgcgta
     7921 attatccaat aatgttaaat ggtatttttg tttcaacaaa gactgcgttt catgttggcc
     7981 atgaatatct aataattctt gcataacttt tcgtaaatct tgtaaagtaa ctgtttgatt
     8041 attaatttta caaagacttt taccagagct aaaaatttcc cgtttaacta ataaaaaatc
     8101 ttcatctaca tcaatatcca tatttttcaa tatatgtata gcatctttac tctcgtcaat
     8161 atcaaatata ccttcgatga cagccttttt ttcaccatgt cttacaaaat cagatgaagc
     8221 tctcattcca attaattgtc caattgcatc tataataatt gacttacctg aacccgtttc
     8281 accacttaaa acagttaaac catcagaaaa ttgaatttct aattcttcaa taatagcaaa
     8341 ttgcttgatt gataaggttt gtaacataaa ctcatcgcat ccttataaca aattgaaaat
     8401 tcttgacttg atttcatcac ttgcctcttt gcttcgacaa ataattaaac aagtatcatc
     8461 accacaaatt gtgcctagta cttcttccca attgatttgg tctaatatag ctccaataga
     8521 ttgtgcatta ccaggtaatg tttttagaac aagtaaatta tcagtaccat ctatattaac
     8581 aaaggaatcc attaaataac gtcccaattt ttctaaagga tggaattttc tatcatttgg
     8641 taaactataa acatattgac ctgaaggtat aggtacttta ataagttgta gttctttaat
     8701 atcacgagaa acagttgctt gagtgacatt taaatcataa tcgtttaatc gtttaactaa
     8761 ttcatcttgt gtctctatct gttcatttga aataatttct ctaattttta tatgcctaac
     8821 cgattttttg ggcacagtaa acacctctga ttacgaatat ttatacattt attttaacac
     8881 atgcactgat ttacgactac taaacacctt tacgtaaaaa gggtaaacat ggtttatcta
     8941 tcttggttat ctatttataa atatt
//
LOCUS       DAHHSC010000095         8909 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.096, whole genome shotgun sequence.
ACCESSION   DAHHSC010000095 DAHHSC010000000
VERSION     DAHHSC010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 8909)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8909)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8909
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.096"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            complement(347..916)
                     /locus_tag="NBI57_001650"
     CDS             complement(347..916)
                     /locus_tag="NBI57_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HCD1822589.1"
                     /translation="MSELTAKQARFVNEYIRTLNVTQSAIKAGYSANSAHVTGCRLLK
                     KPHIKQYIQEQKDKIIDENVLTAKELLHVLTNAAVGDETETKEVVVKRGEYKENPQSG
                     KVQLVYNEHVELIEVPIKPSDRLKARDMLGKYHKLFTDKHDINGNVPIFINIGEWDGD
                     DEELDKAVKDVSNANPNHTVIVDDIPLED"
     gene            complement(913..1125)
                     /locus_tag="NBI57_001651"
     CDS             complement(913..1125)
                     /locus_tag="NBI57_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pathogenicity island family protein"
                     /protein_id="HCD1822590.1"
                     /translation="MADDNIDDHIVKNHLEMIVDRVANDKEFYIFDSLIQGRSFKDIS
                     NVLECSEQSVRLWYETLLDKIVEVIE"
     gene            complement(1257..1784)
                     /locus_tag="NBI57_001652"
     CDS             complement(1257..1784)
                     /locus_tag="NBI57_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017804627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat protein"
                     /protein_id="HCD1822591.1"
                     /translation="MKLSKSKNVLYYRNNGNKLSEYQLLTQFNPAFINKKIKMCEFQI
                     ESMYHMSASTTTCDEIMGVVSVSYPIEKLVIKIIETKAGLQNYKNRSINNTALLKKVL
                     NHYTEKEQKQVVKYMRSNGRYKPYNVIERLQVDLYQASIKQRSERQKQRNTAIENSKI
                     ARVNAYHQSSYVKVV"
     gene            complement(1837..2013)
                     /locus_tag="NBI57_001653"
     CDS             complement(1837..2013)
                     /locus_tag="NBI57_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001160677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1822592.1"
                     /translation="MQFSKTLEAINNAELDENQRLSILEALKEDVNDEEKRNVRDVPT
                     ISDLADKVNIRKNK"
     gene            complement(2013..2591)
                     /locus_tag="NBI57_001654"
     CDS             complement(2013..2591)
                     /locus_tag="NBI57_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000856047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1822593.1"
                     /translation="MKTNHLFEKYSDEVKGYKEEINNLESKIENTTKTIEDLSSKYKE
                     YIKVGNDNEADKTFNKISKLEDEKAKDNKRFEIKKELFNSIKREKLIDLLLNRKNIPE
                     LYQEEAQSLARELEGTIKQFNNVIDKINNMNEEYREDMYKFDSLIDQNEMKKDNLFRQ
                     RYGEVIVLYLNNFLINTKSIRFNAHKKLEVKK"
     gene            complement(2626..2967)
                     /locus_tag="NBI57_001655"
     CDS             complement(2626..2967)
                     /locus_tag="NBI57_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001668138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pathogenicity island protein"
                     /protein_id="HCD1822594.1"
                     /translation="MQSIAEKETYHLPTEHLQVFNVIKNTSNKYITKTKILNQLGYEV
                     NKANNRWLTQVITSLIINYHYPIGYSYKKDTRGYYIIKTQADKIEAIKSIKGLIEGSQ
                     NRLKALEEIEV"
     gene            complement(3417..4058)
                     /locus_tag="NBI57_001656"
     CDS             complement(3417..4058)
                     /locus_tag="NBI57_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012990921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pathogenicity island protein"
                     /protein_id="HCD1822595.1"
                     /translation="MNIETIVNQFETRAGTLLRYYTGLLEHSKVQPCCFKLYNDPFDM
                     VYVMMNSKLFGHVYIKDCKVRQSFELASPKHTEGLIRSIEGHYVGYELHDGKHLSISD
                     MMASHLFEDEYFMYGLQTYAESNNSDVFEYLENGFDTDTLEGIQSSNTDVIANIEMLY
                     QLATGINEPEPELVEGLKLVTEFIQDENATQEDYKALERKLNDLKASYYSLSK"
     gene            complement(4060..4332)
                     /locus_tag="NBI57_001657"
     CDS             complement(4060..4332)
                     /locus_tag="NBI57_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001149387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pathogenicity island protein"
                     /protein_id="HCD1822596.1"
                     /translation="MQAVKEDYNLDEQAQRIGLITGISNEIYYCSISYLSTVYLEYID
                     NTWTAWRESYIPKLNKRTSYKVIASGSFELVLARLKSYLNYIKRSK"
     gene            complement(4319..5122)
                     /locus_tag="NBI57_001658"
     CDS             complement(4319..5122)
                     /locus_tag="NBI57_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA primase/polymerase"
                     /protein_id="HCD1822597.1"
                     /translation="MTGYHVAKHLLSKNIEVIPLNNHKKPTVSFADIDITDEFVEYHS
                     NIYHQTNVLGVLTRGVWCIDIDVDHEDGKNGFDSLKQIPYYEELVTNAQNTLVQTTAS
                     GGKHIIFKKHDNIEYGQKIGYLPSVDIKAHPNNYFVLAGSQTAKGIYTHNGVNVTEYQ
                     GEFEKRIFSKAGNYKQQVLEPYSIRRALPNYSFSHVRVGKGGEGKRAYQRIIDGQSEY
                     RNNDLYKAVSYAIQCNVDIEPLRVLIGDNKNGDVFTERDWEATVRSASR"
     gene            complement(5298..6914)
                     /locus_tag="NBI57_001659"
     CDS             complement(5298..6914)
                     /locus_tag="NBI57_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage resistance protein"
                     /protein_id="HCD1822598.1"
                     /translation="MDEVSLYKKHFEFHSKLDYVSTINLSRIKEISKRINFASISTDR
                     QVFNNKGNAYHREKDNVAGDYISNLTLDYTIKPKEIGLVYGTVNIKTVDKNGEEEKQS
                     TFKTSHFHNYARFIADLISDRVIYSKQLDCFIIVKNNQYEVIDNTNFALTYPVDNKYY
                     IDDFLDVMLELYREHLNVTHNFKIYPYCIAGNDWVYDCQNLTLTEQKLKSDELYSIKY
                     DVDIKDINLEIPRNFFDLVTDNKKSKNNLMLTHAYTMYRKMKLIQAEKWFLLKDFGRS
                     GKGLFMATFEKLMTVNKVNFDSLISGGFESANEWMNFYGADIAHANETGEITKQMMRI
                     LRKIATGETISGRGIGRNAFTFKNNAVLILDTNESVDTGEITANTTRTVKISLKDRPI
                     NETDEERYQIFKPYWDFIQPNGNTSVNASVSFLITSLEYLKENGREFKFKDVTLKYYF
                     NEDELTETQITMIRLLSKQGFILAGDETLQRLIEQDYSSLRYKNAKEDMRKIGISINN
                     QKKIDGVNTKVHKVGNIELFNMALELISEH"
     gene            complement(6959..7282)
                     /locus_tag="NBI57_001660"
     CDS             complement(6959..7282)
                     /locus_tag="NBI57_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001103972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1474 family protein"
                     /protein_id="HCD1822599.1"
                     /translation="MNWEIRNLMCNIEIVKEKLEDVATTHTWFVDGRFTKRSLKTKEE
                     VVNYGLAYNEHRIHNEQVTELMLTYLEELDGLMNKFHEIEKASLSTDQSESNANVQSI
                     YLNKY"
     gene            complement(7283..7666)
                     /locus_tag="NBI57_001661"
     CDS             complement(7283..7666)
                     /locus_tag="NBI57_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000403836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pathogenicity island protein"
                     /protein_id="HCD1822600.1"
                     /translation="MEIKQKYQLSKVVKILEVVLYEEDKFKSDKDYHYQDKAFYEYAL
                     KLVHNGLFNILAELDFEDEAFLILDEVTMTLSDVMKETQHVYRYSVIDEKGEHKHTTD
                     RKGHVIGMLEWALDYIVGNIEVEEL"
     gene            complement(7668..7871)
                     /locus_tag="NBI57_001662"
     CDS             complement(7668..7871)
                     /locus_tag="NBI57_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001231389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pathogenicity island protein"
                     /protein_id="HCD1822601.1"
                     /translation="MRKQVIITKTVVGWYNIKDTQHNLMLNIPPKVFEQYFPDVSKDV
                     QVACLEMDLSKITEIKNKKKVGS"
     gene            complement(7838..8011)
                     /locus_tag="NBI57_001663"
     CDS             complement(7838..8011)
                     /locus_tag="NBI57_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000784884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pathogenicity island protein"
                     /protein_id="HCD1822602.1"
                     /translation="MKMYLTYICLVSLLTILLLAISNMYVAFSVYGMMVTYGFNLTGE
                     ITTCENKLLLQKQ"
     gene            complement(8008..8235)
                     /locus_tag="NBI57_001664"
     CDS             complement(8008..8235)
                     /locus_tag="NBI57_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000164237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCD1822603.1"
                     /translation="MTKLTYPMLYITRKEKGDTQKKVASKLGISPQRYQLKESGKAIF
                     NLNECQILSEMYDMPIDELFSSKIKVEGSRQ"
     gene            complement(8271..8477)
                     /locus_tag="NBI57_001665"
     CDS             complement(8271..8477)
                     /locus_tag="NBI57_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002505677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1822604.1"
                     /translation="MNNKVSNYRRYIGLTQNQMANNLKISVTAYRNKERGITPFKDSE
                     KVFIKNKLIENGIKDITIDDIFFN"
     gene            8698..>8909
                     /locus_tag="NBI57_001666"
     CDS             8698..>8909
                     /locus_tag="NBI57_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000390815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCD1822605.1"
                     /translation="MEEFGEKFTHKAHKSIVSKWEKGLTKPSNERLKEIAKLGNISVH
                     QLIYGDFLGLLESIANEEIKFILDTN"
BASE COUNT         2676 a         1658 c         1101 g         3474 t
ORIGIN      
        1 tataaataga taaagaccat tacctaaatc agatggtaat ttattaacag ggaattcctt
       61 atttatttct aaattatcat agcaacttct atctgtacca tgataacccc ttatatctat
      121 tgttgtgcac atagtaatac tccttatgaa aaacaattgg gtttaattat acaactatac
      181 aagcagatta taaagccttt tacttttaat cactatcata aagtctaata aacgctaaat
      241 cgcataggct tggcgtctat ttacattact cctcaaaccc acctatatca tcaacaattt
      301 ctggtgtcac aagactcaca tctagtttat ctgtaaatat tcctcattaa tcctctaacg
      361 gaatatcatc cacaatcaca gtatgattag gattagcgtt agatacatct ttcactgcct
      421 tatctaattc ctcatcgtct ccgtcccatt caccaatatt aatgaatata gggacattcc
      481 cgttaatatc atgcttatct gtaaataact tatggtattt ccccaacata tcacgagctt
      541 ttaaacgatc actaggtttt attggtacct ctatcagttc aacatgttca ttatagacta
      601 attgtacttt gccactttgt ggattctctt tatattctcc acgctttact acaacttctt
      661 tcgtttctgt ttcatcaccg actgccgcat tcgtaagcac atgtagtaac tcttttgcgg
      721 ttaatacatt ctcatctata atcttatctt tttgttcttg tatatattgc ttgatgtgtg
      781 gcttcttcaa taacctacac cctgtcacat gtgcgctatt tgcgctatag cctgctttta
      841 tggcactttg tgttacatta agtgttctta tatactcatt cacaaaacgc gcttgttttg
      901 ccgttaactc actcattcta tcacctccac aattttatct aataaggttt cataccataa
      961 tcttacagat tgttctgaac actctaagac attgctaata tctttaaaac tacgtccttg
     1021 tattaaagaa tcgaaaatat aaaactcttt atcattagct actcggtcaa caatcatttc
     1081 taagtgattc tttacaatat gatcatcaat gttatcgtct gccatccatt cattggaatt
     1141 ttcatcacct attgaaaaga attcatcagt atttatataa tcatctatta atacatcact
     1201 tctagttcgc tcatgataat cacaaacgaa gtcttttatt tgctttttat ccattgttac
     1261 accactttta catatgaaga ttggtgatat gcatttactc gtgcaatctt actgttttca
     1321 attgctgtat ttctttgttt ttgacgttct gaacgttgtt taatacttgc ttgatacaaa
     1381 tcaacttgta agcgttcaat gacgttgtag ggcttatatc gtccatttga acgcatatat
     1441 tttacaactt gcttctgctc tttttctgta taatgattta gtaccttttt caacaacgcc
     1501 gtattattta tagatctatt tttatagttt tgtaaccctg cttttgtttc aataattttg
     1561 ataactaatt tttcaatcgg atatgagaca gacacgaccc ccattatttc atcacatgtt
     1621 gtggtcgacg cactcatatg gtacatactt tcaatttgga attcacacat cttaattttt
     1681 ttattaataa atgctgggtt aaattgcgtt aatagttgat actcggatag cttattgccg
     1741 ttattacgat aatataaaac gttcttagat ttactcagtt tcatttatcc accccactac
     1801 ttaataaagc caaaccaatt aaggcttggc ttttgtctat ttgttttttc taatatttac
     1861 tttatcagct aaatctgaaa tagttggaac atctcttacg tttctttttt cctcatcgtt
     1921 cacatcttct ttaagtgctt ctaaaataga taagcgttgg ttttcatcta attcagcatt
     1981 gtttattgct tctaatgttt tactaaattg cattatttct taacctccaa ttttttgtgt
     2041 gcattgaaac gaattgattt tgtattaata agaaagttgt tgaggtaaag tacaatcact
     2101 tcgccatatc gttgtctaaa taaattatct tttttcattt cattttgatc tatcaaggaa
     2161 tcgaacttat acatatcttc tcgatattct tcattcatat tgttgatttt atcaataaca
     2221 ttattaaact gtttgattgt accttctaat tcacgcgcta aactttgtgc ttcctcttga
     2281 tataactcag gaatattctt cctattcaac aataagtcta tgagtttttc gcgcttaatg
     2341 ctgttaaata attccttttt aatttcaaat cttttattat cttttgcttt ctcatcttct
     2401 aattttgaaa tcttattaaa cgtcttatca gcctcattat cgttgccaac ttttatatac
     2461 tctttatatt tggaagatag atcttcaata gtttttgtag tattttcaat tttagattct
     2521 aaattattaa tttcttcttt gtagcctttt acttcatcgg aatatttttc aaataaatga
     2581 tttgttttca tttatgttac cctctttcat ttcatagtta tcatgttaca cttcaatttc
     2641 ttctagggct ttcaaacggt tctgactgcc ctcaattaag cccttaatac ttttgatagc
     2701 ttctatctta tcagcttgtg ttttaatgat gtaatagcct ctagtatctt ttttatagct
     2761 atatccgata ggataatgat aattaatgat taagcttgta atgacttgtg ttaaccatct
     2821 attgttagcc ttattcactt catatcccaa ttgatttaag attttagttt tagtaatata
     2881 cttattggac gtatttttta tcacattgaa aacttgcagg tgttcggtgg gtaaatgata
     2941 cgtctctttt tctgcgatac tttgcatttc tacacctctt tcttttaatt atttcatacc
     3001 taaattatac catttttaca ggtccaaaac aaacttacgt tcgctttata gcgcgtttta
     3061 tcaattgttt agcttatcat atataacact tataaaatca tgttataaac ttaacgttag
     3121 gcttttcaca ttaacctaat atagaactta agttcggtaa aataacacga acaaacagcg
     3181 aacaaactta acttttaggc ctatgccaaa aacacaaact ttagcttgta ttagcgttaa
     3241 caaagttcgc acaccttgca caaatcttgc cattttttca attctcaaag actgtatacc
     3301 ttccgatttt aaaagccaac accttccgaa aaccttacca ttttaaactg ctatacctcg
     3361 tataaaatcg tagtatttta ttaggagcca cacactacat gtgacccctc ataacattat
     3421 ttactcaagc tatagtaaga cgcttttaga tcattcaatt tacgttctaa agccttgtaa
     3481 tcctcttgcg tcgcattctc atcttgtata aactcagtta ccaacttcaa tccctcaact
     3541 aactctggtt ctggttcatt gattcccgta gctaactgat acaacatttc aatattcgct
     3601 atcacatcag tattacttga ttgaatgccc tcaagtgtat ctgtatcaaa tccattttct
     3661 aggtactcaa acacatcact attatttgat tctgcatatg tttgtaatcc atacataaaa
     3721 tactcatctt caaacaaatg actagccatc atatcactaa tagaaagatg tttaccgtca
     3781 tgtaattcat aacctacata atgcccctct atgcttctta taagcccctc agtgtgctta
     3841 ggtgacgcta attcaaatga ttgccttact ttacaatctt taatatatac atgaccgaat
     3901 agcttgctgt tcatcatcac gtataccata tcaaatggat cattgtataa cttaaagcaa
     3961 cacggttgca ctttactatg ttctaataat cctgtgtagt accttagtaa cgtgcctgct
     4021 cgtgtttcaa attggtttac aatagtttct atgttcatat cacttactcc tttttatata
     4081 atttaaataa ctctttaatc tagctagcac taattcaaaa cttcctgaag ctataacttt
     4141 gtaacttgtt cttttattta atttaggaat ataactctca cgccatgcag tccaagtgtt
     4201 atcaatatat tctaaataaa ccgttgataa ataacttatt gaacaatagt atatttcgtt
     4261 agatatacca gttattaaac caatcctttg agcttgttcg tctaaattgt aatcctcttt
     4321 aacggcttgc acttctaaca gtcgcctccc aatctctctc cgtaaataca tcgccgtttt
     4381 tattatctcc aatcaataca cgtaacggct caatatctac gttacattga atcgcataac
     4441 ttactgcttt atataaatca ttgttcctat attcactttg accgtctata atgcgttgat
     4501 atgcacgttt tccttctcca cctttgccaa ctcttacgtg actaaaacta taattaggta
     4561 gtgctcgtcg aatggaatat ggctctaata cttgttgttt gtaattacca gctttagaaa
     4621 atattcgttt ctcaaactct ccttgatact cagttacatt gacaccgtta tgagtgtata
     4681 tacctttagc tgtttgacta cctgcaagca caaaataatt attgggatgt gctttgatat
     4741 caacagatgg taaataacct atcttctgtc cgtattcgat attgtcatgc tttttgaata
     4801 tgatatgttt cccaccactt gccgttgtct gtactaatgt attttgtgca ttggtaacaa
     4861 gttcttcgta atatggtatt tgtttcaaac tatcgaaacc attcttacca tcttcatgat
     4921 ctacatcaat gtcgatacac catacacctc gtgttaatac gcccaataca ttggtttgat
     4981 gataaatatt agaatgatat tcaacgaatt catcagtaat atctatatca gcaaatgaaa
     5041 cagttggctt tttgtgatta tttagtggta taacttcaat attcttactt aataagtgtt
     5101 tcgctacatg ataacctgtc attgtatacc tcctttgtag gtagccagta actctaataa
     5161 ctcttatttt tacctatatc atcaacaaca ttaaaagtta tcgattaatg ttacagtaat
     5221 aagagttata taggttacca attgttataa cgccattttt agagttacta tataagttac
     5281 caagagttac agtaacctta gtgttcagaa attagttcta aagccatgtt aaataattca
     5341 atgtttccaa ctttatgaac ttttgtattt accccgtcta ttttcttttg attattgata
     5401 ctaatgccaa ttttcctcat atcttcttta gcgttcttgt aacgtaaact tgagtaatct
     5461 tgttctatta agcgttgtaa tgtttcatca cctgctaata taaagccttg ttttgataac
     5521 aatctgatca tagtaatttg agtttcagtc aattcatctt cattaaaata atacttgagc
     5581 gttacatctt taaatttaaa ttctcgccca ttttctttta aatattccaa actcgttatt
     5641 aagaatgaca cagacgcatt aactgaagtg ttgccattag gttgtataaa atcccaatac
     5701 ggcttaaata tctgataacg ttcttcatca gtttcattta taggtctatc ttttagtgat
     5761 attttaactg ttcgtgttgt attagctgta atttcaccag tatcgacact ttcatttgta
     5821 tctagtatta atacggcgtt atttttaaat gtaaatgcgt ttcttccaat gccacgtcca
     5881 gaaattgttt cacctgttgc tatttttctt aatatgcgca tcatttgttt agtgatttca
     5941 cctgtctcat tagcatgagc tatatctgca ccgtaaaaat tcatccactc atttgccgat
     6001 tcaaaaccac cagaaataag gctatcaaaa ttaactttgt tcactgtcat caatttttca
     6061 aatgtagcca taaacaaacc ttttccagaa cgaccaaaat ctttaagtaa aaaccacttt
     6121 tctgcttgta tcaatttcat ttttcgatac attgtataag cgtgtgttag cattaaattg
     6181 tttttactct ttttattgtc agttactaaa tcaaagaagt ttctgggtat ttctaaattg
     6241 atatctttaa tatctacgtc atatttaatt gagtagagct catcactttt taatttttgt
     6301 tctgtaagcg ttaaattttg gcaatcatat acccagtcat tacctgcaat gcaatatgga
     6361 taaatcttaa agttatgagt tacatttaaa tgttcgcggt aaagctctaa catcacatct
     6421 aagaaatcat caatatagta cttgttatca actggatagg ttaacgcaaa gtttgtattg
     6481 tctatcactt catactggtt attcttaact ataataaagc agtctagttg ttttgaataa
     6541 atgaccctgt cagaaattag atcagctata aaacgtgcat agttatgaaa atgactagtt
     6601 ttaaacgtag attgtttttc ttcttcacca tttttatcaa cagtcttgat attgacggtt
     6661 ccataaacaa gcccaatttc ttttggtttt atggtataat ctaaagtaag attactaata
     6721 taatcacccg caacattatc tttttctcgg tgatatgcat ttcctttgtt attaaaaact
     6781 tgtctatctg ttgagattga tgcaaagttt atacgcttgc ttatctcttt tatcctagat
     6841 agattaatcg ttgaaacata atctaattta gaatgaaatt cgaaatgttt tttataaagt
     6901 gatacttcgt ccatgtagtc atcctttcga tatcatttta tttttgttaa tatatttact
     6961 agtatttatt taaataaata ctttgtacat ttgcgttact ttcgctttgg tcggtggaga
     7021 gtgacgcttt ttctatttca tgaaacttat tcattaaacc gtctaattct tccaaataag
     7081 taagcattaa ttctgtaact tgttcgttgt gaattctatg ttcgttatac gctagaccgt
     7141 aattaaccac ttcttcttta gtttttaacg atctcttcgt aaatcgtcca tctacaaacc
     7201 atgtatgtgt agtcgctaca tcttccaact tttcttttac tatctctata ttgcacatta
     7261 aatttctaat ctcccaattc atttataatt cctccacttc aatatttccc acaatgtaat
     7321 ctaatgccca ctctaacatt ccaatcacgt gtcctttgcg atctgttgta tgtttatgtt
     7381 cacctttttc gtctatgaca ctataacggt aaacgtgttg tgtttctttc atgacatcac
     7441 ttagcgtcat tgttacttca tcaagaatta aaaatgcttc atcttcaaaa tctaattcag
     7501 caagaatatt gaacaatcca ttatgaacta attttaaagc atattcataa aatgctttat
     7561 cctgataatg atagtcctta tcggatttaa acttatcttc ctcgtataat actacttcta
     7621 atattttaac cacttttgat aattgatatt tttgtttgat ttccatctta actacctact
     7681 tttttcttat ttttaatttc tgtaattttt gataaatcca tttctaaaca cgcaacttga
     7741 acatctttac taacatcagg aaagtactgt tcaaatactt ttggcggtat atttaacatt
     7801 aaattatgtt gagtatcttt aatgttgtac cagccaacta ctgtttttgt aataataact
     7861 tgttttcgca cgttgtaatc tctcctgtta aattaaatcc ataagttacc atcatgccgt
     7921 acacactaaa agcgacatac atgttagata ttgctagtaa taatattgtt aacaatgaaa
     7981 ctaagcagat ataagttaag tacattttca ttgccttgac ccctctactt taatttttga
     8041 tgaaaataac tcatcaattg gcatgtcata catttctgaa agaatctgac actcatttaa
     8101 attaaatatt gctttaccac tttcctttaa ctggtagcgt tgtggactaa taccaagttt
     8161 gctagcaact ttcttttgcg tgtcaccttt ttcttttcta gtaatgtata acattggata
     8221 agttagtttt gtcattctaa tcactcctaa aggaaacttt atgctactta ttaattaaaa
     8281 aaaatatcat caatagtaat gtctttgata ccattttcta ttaatttatt ttttataaac
     8341 accttctctg aatctttaaa tggtgttatt cctctttctt tatttctata agcagtaact
     8401 gaaattttta aattattagc catttgattt tgtgttaaac caatatacct tctgtaattg
     8461 cttactttat tattcatcaa aaccacctcc ttgaaacttt atgctacaat catataacct
     8521 ctccttttac ttgtcaacac tttttatgat actttatgaa acttataatt ccactaaagc
     8581 taaattaatg atagaatact aacataaata ttcatcatta aggaggcgtt tagtatttca
     8641 caacaatcaa ttttagctaa taatataaaa aaaattagaa aagaattagg tcttacgatg
     8701 gaagagtttg gtgaaaaatt cacacataaa gctcataaat ctattgtttc aaaatgggaa
     8761 aaagggctaa caaaaccaag taatgaaaga ttaaaagaga ttgctaagtt agggaatata
     8821 agtgtacatc aattaatata cggtgatttt ctagggttgt tagaaagtat tgcaaacgag
     8881 gaaataaagt ttattttaga cacaaatat
//
LOCUS       DAHHSC010000096         8889 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.097, whole genome shotgun sequence.
ACCESSION   DAHHSC010000096 DAHHSC010000000
VERSION     DAHHSC010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 8889)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8889)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8889
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.097"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            83..541
                     /locus_tag="NBI57_001667"
     CDS             83..541
                     /locus_tag="NBI57_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1822606.1"
                     /translation="MKKLLITIVIIVLIGALGFAIYAAINHSTSSSNNQEQENKSTHK
                     KTETEENDQQDDSADKTDKNQNNGNNDTNDNQPSSPSNVPNNHSTVPSNTQPAQPKDS
                     NSNKNNTHGGGSQSSTNNQNNQQQNTPPANKSNNTNTPSKQTPQAQSPKN"
     gene            781..2655
                     /locus_tag="NBI57_001668"
     CDS             781..2655
                     /locus_tag="NBI57_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HCD1822607.1"
                     /translation="MLDRHLKLLQFFIKNPSKHISSNEIAEHVNVSNRTVRNDIHVIN
                     SNFMDDIIVSIKSKGYQLNTSQYTLETITERYTHIQSYKEKLLLSMAYQLLMHNKSQT
                     LQQLEQDYLLSKTVLNDYFVRIQQWCQKFNIVLTIKKKQGIVVDGTDNDITNAIIHLN
                     QLSSGHVHVEDLILNELPDSHQRMISHIIQETLQQHSIETTDIQIQQLLIHLILIIKR
                     KQSLSELDTLNHDAKLISQSCIKQINERLGYDLNEKVINMFSFFIAYHFNKLDIGIER
                     IFIQSYIDRLIELMQQRIHVPFNEDSILQQNLYNHFSKAYLRITQNIYLNNPLVIEIK
                     KLYPFVFNTLFEAIDKLAIDTDIEMSEDEIAFLTIHFQAAIERRTKTQLNVVIACYYG
                     LGVSNFLETKINNLSEELSVINTIKLESITHYHFDNVDLLITTHDIPKQTLNILPKHL
                     TTIKVAPLFSEDDRHKIRHVVKQKQNPVQAHHHMDTVNFLVVNTEQKSRHTVQIFEEA
                     QKILQAHHAIVEGYIESALEREKSSSTYIGNFMAIPHGDPEKVLQSHVLIFRTKDVFP
                     WRQHDVKLVFFLAISHKDKAFTKQMMQLIANLDDDSVNHLCSLDDHSLKQQLFEYLQE
                     "
     gene            2731..4683
                     /locus_tag="NBI57_001669"
     CDS             2731..4683
                     /locus_tag="NBI57_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIABC"
                     /protein_id="HCD1822608.1"
                     /translation="MKIVAITSCPNGIAHTYMAQEKLEQVAKEMGVDIKVETQGGVGA
                     ENVLTTQDIEEADGVIIAADKQVDLSRFVGKRLINENVREGIHNPRGLIQRIINQDAP
                     IYQSETNYHSKDRGKSKSGIQMVYQHLMNGVSFMVPFIVVGGLLIAIALTLGGETTSK
                     GLVIPDDSFWKSIENIGSLAFKFMVPILAGYIAVSIADKPGLVPGMIGGAIAADGSFY
                     GSDAGAGFLGGIVAGFLAGYIAKWIKDIKVPKAMAPIMPIIIIPIISSVVVGLIFIFL
                     IGAPISNIFEALTTWLKSMQGANIIILAMIIGAMIAFDMGGPVNKVAFLFGSALIAEG
                     NYAVMGMVAVAVCTPPIGLGLATFVQKYKFNHSEREMGKASFTMGLFGITEGAIPFAA
                     QDPLRIIPANIIGAMIASVIAAIGGVGDRVAHGGPIVAVLGGIDHVLWFIFAVIVGSL
                     VTMTTVLLLKRNTPVIAVDAPAQHTQLHDTDITQHDTEVDNVDKTAKHSHLNKPSHVF
                     DQQTMIITDHDMSRNEAIDMLIHKLKICRYVEHTSHLKNAILEREMESTTAIGMNVAI
                     PHAKSDVVKQPIVAVMKNNHGVKWDSLDGSLPQLIFLIAVPNNSQDTHLKILQRLSKA
                     LMNDETRQSLINANSTTEIYNLLMKI"
     gene            4697..5635
                     /gene="manA"
                     /locus_tag="NBI57_001670"
     CDS             4697..5635
                     /gene="manA"
                     /locus_tag="NBI57_001670"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HCD1822609.1"
                     /translation="MALFLQPVFKDRIWGGHALKAFNYDIPNETTGECWAISAHPNGP
                     NTIINGPYKDMTLDQLWSQHRELFDNDSRDSFPLLTKVLDANDKLSVQVHPDDDYALK
                     HEGELGKTECWYILDAAPGAEIIYGVHAHNKQTLIDMIDNHEFDSLFKRIPVKPGDFY
                     YVPAGTVHAIGSGILILETQQSSDTTYRIYDYDRRDQNGQLRDLHLEQSKDVIELGNH
                     EPNTTPITTHIDTHTVTQFVDNQYFAVYKWDIHGDLKFNKPHAYCLVTIIDGTGTLTV
                     NDKHYDIQKGSSFILTTEDEDIHFVGNISAIVSHPG"
     gene            complement(5747..8728)
                     /locus_tag="NBI57_001671"
     CDS             complement(5747..8728)
                     /locus_tag="NBI57_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
                     /protein_id="HCD1822610.1"
                     /translation="MKNAFKLFKMDLKKVAKTPAVWIILAGLAILPSFYAWFNLWAMW
                     DPYGNTGHIKVAVVNEDKGDTIRGKKVNVGNTMVNTLKKNKSFDWQFVSREKADHEIK
                     MGKYFAGIYIPSKFTHEITGTLRKQPQKADVEFKVNQKINAVASKLTDTGSSVVVEKA
                     NEQFNKTVTRALLEEANKAGLTIEENVPTINKIKNAVYSADKALPKINDFANKIVYLN
                     NHQADLDKYANDFRKLGNYKGDILDAQKKLNEVNGAIPQLNEKAKLILALNNYMPKIE
                     KALNFAADDVPAQFPKINQGLNIASQGIDQANGQLNDAKGFVTQVRSRVGDYQDAIRR
                     AQDLNRRNQQQIPQNSAANNETSNSSPAAGNGVASTPPSAPSGDTAPNNNVTQNTAPN
                     SNNAPVSTTPQSTSGKKDGQSFADITTTQVSTANENTQNITDKDVKSMEAALTGSLLS
                     LSNNLDTQAKAAQKDSQALRNISYGILASDKPSDFRESLDNVKSGLEYTTQYNQQFID
                     TLKEIEKNENVDLSKEIDKVKAANNRINESLRLVNQLSNALKNGSSGTAEATKLLDQL
                     SKLDSSLSSFRDYVKKDLNSSLVSISQRIMDELNKGQTALSNVQSKLNTIDQVINSGQ
                     AILKNGKTRIDRLQTVLPSIEQQYISAVKNAQANFPKVKSDVAKAANFVRNDLPQLEQ
                     RLTNATASVNKNLPTLLNGYDQAVGLLNKNQPQAKKALSDLADFSQNKLPDVEKDLKK
                     ANKIFKKLDKDDAVDKLIDTLKNDLKKQAGIIANPINKKTVDVFPVKDYGSGMTPFYT
                     ALSVWVGALLMVSLLTVDNKHKSLESVLTTRQVFLGKAGFFIMLGMLQALIVSVGDLL
                     ILKAGVESPVLFVLITIFCSIIFNSIVYTCVSLLGNPGKAIAIVLLVLQIAGGGGTFP
                     IQTTPQFFQNISPYLPFTYAIDSLRETVGGIVPEILITKLIILTLFGIGFFVVGLILK
                     PVTDPLMKRVSEKVDQSNVTE"
BASE COUNT         2902 a         1594 c         1455 g         2938 t
ORIGIN      
        1 tttaaaatga ttatggcaat attgaaataa attatttagc ttcacgtatc aactactttt
       61 cattaacatg gaggcgttat atttgaaaaa acttcttatt accatcgtaa ttatcgtatt
      121 aatcggagca cttgggttcg caatttatgc tgcaataaat cattctacat catcaagtaa
      181 taaccaagaa caagaaaata aatctacgca taaaaaaaca gaaaccgaag aaaatgatca
      241 acaagatgat agtgcagaca aaacagataa aaatcaaaat aatggcaata acgatactaa
      301 tgacaatcaa ccatcttcac cgtcaaatgt tcctaacaac cattcaacag taccatcgaa
      361 tacgcaacca gctcagccaa aagactcaaa ttcaaataaa aataatactc acggtggtgg
      421 cagtcaatcg tcaacaaaca accaaaataa tcaacaacag aacacaccac ctgctaataa
      481 atcaaataat accaatacgc cttctaaaca aacacctcaa gcacaatcac ctaaaaatta
      541 atcatttgcc tgatacgtcc acaagtacca gatgcataaa ttcagaaaaa cagcagtgag
      601 atatttttct atttgaaata tcttactgct gttctttatt tgcataattc gcactctgtt
      661 aaaatcatat aaaagttcgc tttaatgtca ttgacgtatg tataagctct catgtttaat
      721 aatattaata cacctttaaa aacgcttaca cgaaaaattt agaaaggagg tacatgttaa
      781 atgcttgata gacacttaaa actactccag ttttttatta aaaatccatc aaagcacatt
      841 agttctaatg aaattgctga acatgtcaat gtttcaaacc gcacagttcg caatgatatt
      901 catgttatca atagcaattt tatggatgat atcatcgtaa gtattaaatc taaaggatat
      961 caattaaata catcgcagta taccttagaa acgataactg aacgttacac gcatattcaa
     1021 tcatataaag aaaaactatt attatctatg gcgtatcaac tgctaatgca caataaatcg
     1081 cagacattgc aacaactaga gcaagattat ctattatcta aaactgtgct caacgactat
     1141 ttcgttcgaa ttcaacaatg gtgtcaaaaa tttaatatcg ttttaacaat caagaaaaaa
     1201 caaggtattg tggtcgatgg aacagataat gatatcacta atgccatcat ccatttaaat
     1261 caattatcat cagggcatgt acatgttgaa gacctaattt taaatgaatt acctgattca
     1321 caccaaagga tgatttcgca tattatacaa gagacacttc aacaacatag tattgaaaca
     1381 actgatattc aaatacaaca gcttttaatt catctcattt taatcattaa acgtaagcaa
     1441 tctttaagtg agctagacac gttaaaccat gatgcaaaat tgatatctca atcttgcatt
     1501 aagcaaatca acgaaagatt aggttatgac ttgaacgaaa aagtaatcaa tatgttttca
     1561 ttctttattg cttatcattt taataaactc gatattggta tagaacgtat atttatacaa
     1621 agttatattg atcgcttaat cgaattgatg cagcaacgta ttcatgtacc tttcaatgaa
     1681 gattcgattt tacaacaaaa tttatataat cattttagta aagcatattt acgtatcact
     1741 caaaacattt atttaaacaa tcctttagtt atagagatta aaaaactcta tccctttgtg
     1801 tttaacacac tttttgaagc gattgataaa ttagcaattg atactgatat agagatgagc
     1861 gaagatgaaa ttgcattttt aacaattcat tttcaagctg ccatcgagcg tcgtaccaaa
     1921 acacaattaa atgtagtcat tgcttgttat tatggtctag gtgtgtcgaa ttttttagaa
     1981 actaaaataa ataacttatc cgaagagttg tcagtaataa atacgattaa actagaaagt
     2041 ataacacact atcactttga caatgtagat ttattaataa cgacccatga tattccaaaa
     2101 caaacgctaa acatacttcc taagcacctg acgactatca aggttgcacc attattttct
     2161 gaagatgatc gtcacaaaat cagacacgtt gtgaagcaaa agcaaaatcc ggttcaagca
     2221 catcatcata tggacactgt caatttcctt gtcgtaaata ctgaacaaaa atcacggcat
     2281 actgtgcaaa tttttgaaga agctcaaaaa atattacaag cacatcatgc gattgtagaa
     2341 ggatatatcg aatcagcttt agagcgtgag aaatcatctt ctacatacat aggtaatttt
     2401 atggcgattc ctcatggaga tcctgaaaaa gttttacaat cacatgtgct tatttttcgc
     2461 acaaaagatg tttttccttg gcgacaacac gatgtcaaac ttgtcttttt cttagcgatt
     2521 tcgcataaag acaaagcttt tacaaaacaa atgatgcaat taattgctaa cttggatgat
     2581 gattcagtga accatttatg tagtttagat gatcatagtt taaagcaaca gttatttgaa
     2641 tacttacaag aataattgcc actagtaacg gtaattattc tttttttagt atcgagaata
     2701 cgcttacaat atagaaaagg agatgatttt atgaaaattg tagctattac ctcatgccca
     2761 aatggcattg cacatacata tatggctcaa gaaaagcttg agcaggtagc aaaagaaatg
     2821 ggtgtggata ttaaagtaga gacacagggc ggtgttggtg ctgaaaatgt tttaactacg
     2881 caagacatcg aagaagctga cggtgtcata attgcagctg ataaacaagt tgatctatcc
     2941 cgatttgttg gtaaacggtt gattaatgaa aatgttcgcg aagggattca taatccgaga
     3001 ggtctaattc aacgtatcat taatcaagat gcgcctattt atcaatctga aacaaattat
     3061 cattcgaaag atcgcggtaa gtctaaaagt ggtattcaaa tggtgtatca acatttaatg
     3121 aacggtgtat cgtttatggt tccttttatc gtagttggtg gactccttat cgccatcgcg
     3181 ctgactctag gcggtgaaac gacatcaaaa ggattagtca tcccagatga ttcattttgg
     3241 aaatccattg aaaacattgg tagtttagcc tttaaattta tggttccaat actagctgga
     3301 tatatcgctg ttagtatcgc tgacaagccc ggtcttgttc ctggtatgat tggcggcgct
     3361 atcgctgctg acggtagttt ttatggcagt gatgcaggcg caggattttt aggtggtatt
     3421 gttgctggtt tcttagcagg ttatatcgca aagtggatta aagatattaa agttccaaaa
     3481 gcaatggcac caattatgcc tatcattatt attcccatta tttcatcggt cgttgtagga
     3541 ttgatattta tatttttaat cggtgcacca atttctaata tttttgaggc attaacaacc
     3601 tggttaaaaa gtatgcaagg tgctaatatt attatacttg cgatgattat aggtgcgatg
     3661 atcgccttcg atatgggcgg tccagtaaac aaggtagcat ttttatttgg ctcagcactt
     3721 attgctgaag gtaattacgc cgttatgggt atggtcgcag ttgctgtatg tacaccacca
     3781 atcggtttag gtttagcaac gtttgttcaa aagtataaat tcaatcattc cgaaagagaa
     3841 atgggtaagg cttccttcac tatgggacta tttggtatta ctgaaggtgc tattcctttc
     3901 gcagcccaag atccattgcg cattatacct gcaaacatca ttggtgcaat gattgcatca
     3961 gtcattgcag ccattggtgg tgtcggcgat agagtcgcac atggaggtcc aatcgtggct
     4021 gtattaggtg gtattgatca tgtattatgg tttatctttg cagtgatagt aggtagtctt
     4081 gtcacaatga ctactgtact attattaaaa cgcaatacac cagttatagc agtagatgca
     4141 ccagcgcaac acacgcaact tcatgataca gatattacac aacatgatac agaggttgac
     4201 aatgtagata aaaccgcgaa acattcacat ctcaataaac cttctcatgt ttttgatcaa
     4261 caaactatga taattactga tcacgacatg tctcgaaacg aagcaattga tatgttgatt
     4321 cataaactta aaatttgccg ttatgtagag cacacatcgc atttaaaaaa tgcaatacta
     4381 gaacgtgaaa tggagtctac gacagctatt ggcatgaacg tggccattcc acatgcaaag
     4441 tctgatgttg taaaacaacc gattgtagct gttatgaaaa ataatcatgg tgttaaatgg
     4501 gacagtctag atggtagtct acctcaattg atattcttaa ttgccgtacc aaacaacagt
     4561 caggatacac atttaaaaat acttcagcgc ttatctaaag cgttaatgaa tgatgagaca
     4621 cgccaatcac taattaatgc aaacagtact acagaaattt ataacttatt aatgaaaata
     4681 tagaggagtt ttaactatgg cattattttt acaacccgtt tttaaagaca gaatttgggg
     4741 cggtcatgca ttaaaagcat ttaactatga tatccctaat gaaacaactg gtgaatgctg
     4801 ggcaatttct gcacatccaa atggtcctaa tacaattatc aatggtccat acaaggatat
     4861 gaccctagat caactttggt cacaacatcg tgaattattc gataatgatt cacgagatag
     4921 tttccctctg ctaactaaag tattagatgc caatgataaa ttatctgttc aagttcatcc
     4981 agatgatgac tacgctttaa aacacgaagg cgaactaggt aaaacagaat gttggtatat
     5041 tttagatgca gccccaggtg ctgaaattat atatggcgta catgcacata ataaacagac
     5101 gttaatcgac atgattgata atcatgaatt tgacagtctt ttcaaacgta tacctgttaa
     5161 gcccggtgac ttttattatg ttcctgctgg cactgtccat gccattggtt cgggtatttt
     5221 aattttagag acacaacaat catccgatac gacataccgt atttatgact atgatagacg
     5281 agatcaaaat ggtcaattgc gtgatttgca cttagagcaa agtaaagatg tgattgaatt
     5341 aggcaatcat gaaccaaata ctactcctat aacaactcac atcgacacac acactgtaac
     5401 tcaatttgta gataatcaat attttgcagt atataaatgg gatattcatg gagatttaaa
     5461 attcaataaa ccacatgctt attgcttagt tactattatc gatggtactg gtactttaac
     5521 agtaaacgat aagcactatg acatccaaaa aggttctagc tttattttaa ctacggaaga
     5581 cgaggatatt cactttgtcg gtaatattag tgctatagta agtcatcctg gataatagca
     5641 atgataacaa ccgtcccata aaagacagaa attactatct cttctgtcta aaaacataaa
     5701 aatccacaca ttaaggagct ataaccctaa tgtgtggatt taatttttat tctgtaacgt
     5761 tactttggtc aactttttca gatacgcgct tcatcaatgg atctgttaca ggttttaaaa
     5821 ttaaacctac aacgaagaat cctatgccaa ataacgttaa tataattaat tttgtaatta
     5881 ggatttccgg aacaataccg cctactgttt cacgtaatga atcaattgca tacgtaaatg
     5941 gtaagtatgg cgaaatgttt tggaaaaatt gtggcgtagt ttgaattggg aatgttcctc
     6001 caccacctgc aatttgtaat acaagcaata cgattgcaat tgctttacct gggttaccaa
     6061 gtaatgatac gcacgtatat acgattgagt tgaaaataat cgaacagaaa atcgttataa
     6121 gtacaaataa tacaggtgac tcaactcctg cttttaggat taacaaatct ccaaccgata
     6181 caatgagtgc ttgcaacata ccaagcatta taaagaatcc tgccttacct aagaacactt
     6241 gtcttgtcgt taagactgac tctagactct tatgtttatt atcaaccgtt aataaactta
     6301 ccatcaagag tgcacctacc catactgaca gtgcagtata gaatggtgtc atacctgaac
     6361 cataatcctt aactgggaaa acatcaacag tcttcttatt aataggattt gcaataatac
     6421 ccgcttgctt tttcaaatca ttcttaagtg tgtcgattaa tttgtcgact gcatcatctt
     6481 tgtctaattt cttgaaaatt ttattcgctt ttttcaaatc tttttcaaca tcaggcaatt
     6541 tattttgaga aaaatcagct aaatctgata aagccttttt cgcttgtggc tgatttttat
     6601 ttagtaatcc taccgcttga tcataaccat ttaataacgt tggtaaattt ttattcacac
     6661 ttgctgtcgc attagttaat cgctgttcca actgtggtaa gtcattgcgc acaaagttag
     6721 cagctttagc tacatcactt ttcactttcg ggaagtttgc ttgagcattt ttaacagcac
     6781 taatgtattg ttgttcaata cttggtaata ctgtttgtaa gcgatcaata cgtgttttac
     6841 cattttttaa aatagcttgt ccactgttaa tgacttgatc aattgtattt aatttagact
     6901 gaacattgga taatgcagtt tgccctttgt tcaattcatc cataatacgt tgtgatattg
     6961 atactaaaga gctgttaaga tcttttttaa cataatctct aaatgatgat aatgatgaat
     7021 ctagttttga aagttgatct agtaatttag tagcttcagc agttcctgaa ctaccattct
     7081 ttaatgcatt gcttaattga ttaactaacc ttaatgattc attaattcga ttattagctg
     7141 cttttacctt atcaatttct tttgataaat caacattttc attcttctca atctctttta
     7201 atgtatcgat aaattgttga ttatattgcg ttgtgtattc taaaccggac ttaacattat
     7261 ctaaagactc tctaaaatca gaaggcttgt ccgatgctaa aatgccatac gaaatattac
     7321 gtaatgcctg actatctttt tgtgcggctt tcgcttgggt atctaaatta tttgataatg
     7381 ataataaaga gcccgttaat gccgcttcca ttgatttaac atctttatct gtaatgtttt
     7441 gtgtgttctc gttagctgtg ctgacttgtg ttgttgttat atctgcaaaa ctttgaccat
     7501 cttttttccc gcttgtactt tgtggtgtag tcgatacagg cgcattatta ctatttggtg
     7561 cggtattttg cgtaacatta ttatttggtg cagtatcgcc acttggtgca cttggtggcg
     7621 ttgatgctac accattacca gctgcaggtg aactatttga tgtttcgttg ttcgccgcgc
     7681 tattttgagg aatctgttgc tggtttcttc gatttaaatc ttgcgcgcgt cgaattgcat
     7741 cttgataatc accgactcta cttctaactt gtgtgacgaa gcctttggca tcatttaact
     7801 gtccattagc ttgatcaatc ccttgactcg caatgttaag tccttgatta attttaggga
     7861 actgcgctgg cacgtcatca gctgcaaaat ttaacgcttt ttcaattttc ggcatataat
     7921 tatttaaagc taatatcaac ttagcctttt cattaagttg cggaatagca ccattgactt
     7981 cgtttaattt tttctgagca tctaaaatat cacctttata atttcctagt tttctaaaat
     8041 cattggcata tttatctaaa tccgcttggt ggttattcaa atacacaatt ttattcgcaa
     8101 agtcattaat cttaggtaaa gctttatctg ctgaatatac cgcatttttt atcttgttaa
     8161 ttgtcggcac attttcttca atagttaaac ctgctttgtt agcttcttct aataatgctc
     8221 gagttactgt tttattaaat tgttcattcg ctttttcaac gacaactgac gaaccagtat
     8281 ctgttagctt agacgcaaca gcgttaatct tctgattcac cttaaattct acatctgctt
     8341 tttgaggctg cttacgtagt gtcccggtaa tttcatgtgt aaacttagat gggatgtaaa
     8401 tacctgcaaa atatttaccc atttttatct catgatcagc tttctctcta cttacaaact
     8461 gccaatcaaa acttttattt ttcttgagtg tattaaccat cgtattaccg acattaactt
     8521 ttttccctct gattgtgtcg cctttatctt cattaacgac tgcgaccttg atgtgtcccg
     8581 tgttgccata tggatcccac attgcccata agttaaacca agcgtagaac gatggcaaaa
     8641 tagctaagcc tgctaagata atccacacag ctggcgtctt agctactttc ttcagatcca
     8701 ttttaaataa tttaaatgcg ttcttcattg tcacactcct atgtaggaat tattcatatt
     8761 ttttatatat tttttgtaaa ttaatttatt tttgcgttgt gaattagtat aatcaattta
     8821 ctggaagata tttagtcgat tgatacctat caactatttt cagcatacga taaattataa
     8881 caaatcata
//
LOCUS       DAHHSC010000097         8858 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.098, whole genome shotgun sequence.
ACCESSION   DAHHSC010000097 DAHHSC010000000
VERSION     DAHHSC010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 8858)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8858)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8858
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.098"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            12..1517
                     /locus_tag="NBI57_001672"
     CDS             12..1517
                     /locus_tag="NBI57_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001813848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1822611.1"
                     /translation="MSKKLKIIIPIIIVLLLIGGIAWGVYAFFANTPKNTYLKSEQQT
                     AKMYKDYFNDRFENEVKFQEKMKDNSFLSSLELSADASDEIVKGLGIPKSVVNASKIK
                     MSYGHDPKKEKSMINLEPTIADSALGKFQLAADKDKHYFESPLFKGKYSVNNSDLLST
                     YSKLTGEDEETAKENGITNQQLNLNTLFSNAQAQQSDYSKIAEKYSELIVDKLDDDNF
                     DKGKKEEIKVNGEKYKVRPVTLTLSRADTKKITLAVLEEAKKDKDLKKLMEEQGATKD
                     YDKDIKKAIDDVKETKKDEFAKIQSKIYTEKHTIVKREITITDKENNKTKIKGTNTLE
                     DDKLKLDYALDFDQDKYTYAEAKYTIKGVSSKEKDNKYSDKYEFGKKTEYDESKIKLD
                     NQEKVDGTKRQDKGKITVALDKYSDENEFTFENNIDSDVKNNTQKSTLNIGIKYAEEP
                     INFILKSSTKLKADIDFDDSGAKDFNSLSSKDREKLEKEIEKMAAKCLNQF"
     gene            1602..2768
                     /locus_tag="NBI57_001673"
     CDS             1602..2768
                     /locus_tag="NBI57_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCD1822612.1"
                     /translation="MKTIHLFRIYHSFLLKKWYLIIYLLFILAALLITLTTIQHVTED
                     DNHFNIGVVDKDQSSETKLILNSIGKGSNLGKNVSIKAYDDKQAHTLLKKHKLQGYFV
                     FDKGMTKAFYKQGELPISVYTYDQQSMKSVVLSQLTDSVYQRLMRSMGGILAFQDLAP
                     KASHSDSINVMTDLLITGLNRSGAFNLEPIHLYDTGSYYAITGFLATVFIFALSLFTV
                     LKMNQDTVLKARLKMFHFSKERLLIIRALITWFYTMLWSIVGVVWIVFSIPNTFELYN
                     WPTLAIHLSYYVTFLILWLLLIELLTTGLLNSISKVILAIVILVLSGLTIPTIFLQHI
                     ANGVFNIQPFAVVTNQLLEIILNNYILELHPSFYLSFIALLIINLAVLVWRYRQ"
     gene            2765..3685
                     /locus_tag="NBI57_001674"
     CDS             2765..3685
                     /locus_tag="NBI57_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCD1822613.1"
                     /translation="MKPYIQLVLFKQWLQYILLVTTIVIALVLIGIGYRVAHDNFKIP
                     ITIQDLDQTTASKSFVNKIKQSDYVTIKKVDEDESYIEDDVTKKEAILSMQIPKGFSQ
                     KLKENRLKETIQLYGRDDFIGGIAIEIVSSSLYEQQIPNIIYEHLEDMKQHQSIDAIN
                     KSYHKHTPESKIKFVSLTKQAQHSISISLIFAVILFVSAVQVVLHYRLNQQAALQRLS
                     QYHLSRFKLYSTYVMTHTILLLLVLLAVSLYLSQPLSLIFYLKSLLLILIYEIGIVFI
                     LFHIQTISHRLFMTFIYALAMGIVYLIIFM"
     gene            3699..4358
                     /locus_tag="NBI57_001675"
     CDS             3699..4358
                     /locus_tag="NBI57_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCD1822614.1"
                     /translation="MIEINNLSKRYRNKQIFNHLTMSFDSNRLTVLLGDNGAGKSTLL
                     RMIAGIEKANDGTINYFGEKWNQRQIQNHIGYVPQDIALFEHMTVAENIKFFKSLCKN
                     PINDTTINEYLQQLNFDDTSAKVSTLSGGNKRKINILVGLLGQPRILILDEPTVGIDL
                     KSRHDIHQLLNIMKSKCLIILTTHHLDEVEALADDIKLIGQDPFYQHVLEDKQWAYTY
                     Y"
     gene            complement(4410..5360)
                     /gene="lytM"
                     /locus_tag="NBI57_001676"
     CDS             complement(4410..5360)
                     /gene="lytM"
                     /locus_tag="NBI57_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine-glycine endopeptidase LytM"
                     /protein_id="HCD1822615.1"
                     /translation="MKKLTAAAIATMGFATFTMAHQADAAETTNTQQAHTLMSTQSQD
                     VSYGTYYTIDSNGDYHHTPDGNWNQAMFDNKEYSYTFVDAQGHTHYFYNCYPKNANAN
                     GSGQTYVNPATAGDNNDYTASQSQQHINQYGYQSNVGPDASYYSHSNNNQAYNSHDGN
                     GKVNYPNGTSNQNGGSASKATASGHAKDASWLTSRKQLQPYGQYHGGGAHYGVDYAMP
                     ENSPVYSLTDGTVVQAGWSNYGGGNQVTIKEANSNNYQWYMHNNRLTVSAGDKVKAGD
                     QIAYSGSTGNSTAPHVHFQRMSGGIGNQYAVDPTSYLQSR"
     gene            complement(5686..6678)
                     /locus_tag="NBI57_001677"
     CDS             complement(5686..6678)
                     /locus_tag="NBI57_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="linear amide C-N hydrolase"
                     /protein_id="HCD1822616.1"
                     /translation="MCTGFTIQTLNNQVLLGRTMDYDYPLDGSPAVTPRNYRWTSRTG
                     TTGQTQYGFIGTGTDMEGFIYGDGVNEHGVAISTQYFRGYSSYGSTHKADAMNITQNE
                     IVTWILGYTTSIEDMKQQASQIHVVAVYLNDIGEVPPLHYHVSDATGHSVEVSFKEGE
                     VVIKDNPIGVLTNHPDLDWHYSNLRQYINISPYPATAKLLEGVTIEPLGNEAGTFGLP
                     GGFTSTERFVRMAFMKANIAQNNDKEMDLMNAFYLLDAVNIPIGIVRPHDADNHYTMY
                     QTVINLTTRTLYIKYYGSNEIVALKLTDDLINRKDMTIFKPEKHITIRKLNDNQ"
     gene            6912..8288
                     /locus_tag="NBI57_001678"
     CDS             6912..8288
                     /locus_tag="NBI57_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCD1822617.1"
                     /translation="MNNTQSSPRSNIIIAIMLSALTYWLFAQSFINIGPLVGQTYQTS
                     PAVLNLSISLTSFATGIFMVAAGDIADKIGQLRMTYMGLIISMFASLLLIISDITALL
                     IIGRILQGLSAAILLPSTVGVLNNQFKGEHLRRAISYLMISTVGGIGLAGVIGGLIAT
                     NFGWQMNFIISIVIAFIAILLLKGTPEKVSQHSHRHPFDYKGMSIFAVMIGSFTLLLT
                     QGFEQGWFSTFSFICLSIFIITTLIFIIIERRHEVPFIDFSVLRNRPFIGAFLNNFVL
                     NSGLGVTVVFFIYAQTHLGLSAAQSGLVTLPYAIVAVAMIRLGEKATLRFGGKLMLII
                     GPLFPVIGITIISMTQLSASQYVIAVIIGFVICAIGNGLVATPGLTIAIFSMPNEKVG
                     LATGLYKMSGTLGGAFGIALSTTVFSMLQLNYAPSVAATVTFIVSIVLMILGSLSAYM
                     IIPKTVKS"
     gene            complement(8416..8808)
                     /locus_tag="NBI57_001679"
     CDS             complement(8416..8808)
                     /locus_tag="NBI57_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1822618.1"
                     /translation="MQFKLKEEEIISFLELKYPEKEFEYGRLLVGQHKRDDLDVYYFG
                     DTFLMCTIISFKTFEIKETVELSYDAVNRIVLKDGWLFRKMRIETMQKVLKYGTSKLM
                     LTDFQKENYNKYIQGQKQRVIFENGHFV"
BASE COUNT         2957 a         1526 c         1401 g         2974 t
ORIGIN      
        1 gaggaagcaa aatgtctaaa aagttaaaaa ttataattcc tattattatt gtcttattat
       61 taataggtgg aatcgcatgg ggagtttatg cattttttgc aaacacaccg aaaaatacat
      121 acttaaaaag cgaacaacaa actgcaaaaa tgtataaaga ttattttaat gaccgttttg
      181 aaaatgaagt gaagttccaa gaaaagatga aagataattc gtttttatct tcattagaat
      241 taagcgcaga tgcatcagat gaaattgtta aggggcttgg tattcctaaa tctgttgtta
      301 atgcttcgaa aattaaaatg tcatatggac atgatcctaa aaaagagaaa tcaatgatta
      361 atcttgaacc aacaatcgca gactctgcat tagggaaatt ccagttagct gcagataaag
      421 ataagcatta tttcgaatca ccattattta aagggaaata tagtgttaat aattctgatt
      481 tattatcaac gtattcaaaa cttacaggtg aagatgaaga aacagcaaaa gaaaatggta
      541 ttacaaacca acaactaaat ttaaatactc tttttagtaa tgctcaagca caacaaagtg
      601 actacagcaa aattgccgaa aaatattccg aacttattgt cgacaaatta gatgacgata
      661 attttgataa aggtaaaaaa gaagaaatta aggttaatgg tgaaaagtac aaagttagac
      721 ctgtcacgtt aacacttagc agagctgaca ctaaaaaaat tacattagct gtattagaag
      781 aagctaaaaa ggataaagac cttaaaaaat taatggaaga acaaggtgct acaaaagact
      841 atgataagga catcaaaaaa gcaattgacg atgtcaaaga aactaaaaag gatgaatttg
      901 ctaaaattca atctaaaatt tataccgaaa aacatacgat tgtaaaacga gaaattacta
      961 ttacagacaa agaaaataat aaaactaaaa tcaaaggtac taatacttta gaagacgata
     1021 agttaaaact agattacgca cttgatttcg atcaagataa atacacgtat gctgaagcga
     1081 aatatacaat taaaggcgta tcttctaagg aaaaagacaa taaatacagc gataaatacg
     1141 aatttggtaa aaagacagaa tatgatgaat caaaaatcaa attagataac caagaaaaag
     1201 tagatggcac aaaacgtcaa gataaaggta aaatcactgt cgcattagat aaatatagcg
     1261 acgaaaatga attcactttt gaaaataata tagattctga cgtaaaaaat aacactcaga
     1321 aatctacgtt aaatatcggc atcaaatatg ctgaagaacc aattaatttc attttaaaat
     1381 ctagcacaaa attgaaagca gatattgatt ttgatgatag tggtgcgaaa gatttcaata
     1441 gtctatcttc aaaagaccgt gaaaaacttg aaaaagaaat cgaaaaaatg gcggcaaaat
     1501 gtttgaatca attttaaaaa aggcatctaa ataactaaaa tataaaaata agattgaaag
     1561 gtcatcttgc ctttcaatct tatcattgga ggagtggtta aatgaaaaca atacatttgt
     1621 ttcgcatcta ccactctttt ttattgaaaa agtggtattt gattatctat ttactattta
     1681 tcttagcggc actccttatt acattaacga cgatccaaca tgtaacagaa gatgacaatc
     1741 attttaatat aggtgtcgta gataaagatc aatcaagtga aacgaaatta atcttaaact
     1801 ctattggtaa agggagtaac ctaggaaaaa atgtaagcat taaagcatat gatgataagc
     1861 aagcacatac tttgttaaaa aaacataaac ttcaaggcta ttttgttttt gataaaggta
     1921 tgaccaaggc attttataaa caaggcgaac taccaatttc agtatataca tatgatcaac
     1981 aatccatgaa aagtgtcgtg ctatctcagc taacagattc tgtttaccaa cgtcttatgc
     2041 gatcaatggg tggcatctta gcttttcaag acttagcacc gaaagcatca cattctgaca
     2101 gtatcaatgt tatgactgat ttgctgatta caggattaaa ccgttcaggt gcatttaact
     2161 tagaaccgat tcatttatac gatacgggca gttattatgc aattacagga tttttagcaa
     2221 cggtattcat ctttgcacta tctttattta cagttttgaa aatgaatcaa gatactgtat
     2281 tgaaagcgcg attgaaaatg tttcattttt ctaaagagcg tttattaatc attcgtgcgt
     2341 tgattacatg gttttatact atgttatgga gtatcgttgg tgtagtttgg attgtgttca
     2401 gtattccgaa tacctttgaa ttatataatt ggccaacgtt agccattcat ttaagctatt
     2461 atgttacctt tttaatactg tggttattac tcatcgaact tttaacaaca ggcttgttga
     2521 atagtattag taaagtcata ttagctattg tcatcctggt gttatctggt ttaacaatac
     2581 ctacgatttt tttacaacat atagcaaatg gcgttttcaa tattcaaccg ttcgcagttg
     2641 taacaaatca attgttagaa attattttaa acaactacat tttagaactg caccctagtt
     2701 tctatcttag cttcatcgca cttttgataa ttaatttagc tgtattggtt tggaggtatc
     2761 gtcaatgaag ccttatatac aacttgtttt gttcaagcaa tggttacaat acatcttgct
     2821 cgtaacaacc attgtcatcg cactcgtact tattggtatc ggttaccgtg tagcacatga
     2881 caacttcaaa ataccgatta ccattcaaga tttagaccaa accactgcat caaaatcatt
     2941 cgtcaataaa attaaacaat ctgactatgt aactattaaa aaagtcgatg aagatgaaag
     3001 ctatattgaa gatgatgtta ctaaaaagga agctatttta agcatgcaaa ttcctaaagg
     3061 tttctctcaa aaattaaaag agaaccgttt aaaagaaacg atacagttat atggtagaga
     3121 tgactttata ggtggtattg ctatagaaat tgttagtagt tcattatatg aacagcaaat
     3181 tcctaacatt atttatgaac accttgagga tatgaaacag catcaatcca tcgatgctat
     3241 caacaagtcc tatcataaac atacacctga atctaaaatc aaatttgtgt cgcttactaa
     3301 acaagcacaa cactctattt caattagctt aatctttgcg gtgattttat ttgttagcgc
     3361 tgttcaagta gtacttcatt atcgtttaaa ccaacaagca gcattgcaac gattatcaca
     3421 atatcattta agccgtttca aactatatag tacttatgta atgacacata cgatcttgtt
     3481 attgttggta ctattggcag ttagtctata tttgtctcaa ccactcagct taatatttta
     3541 cttaaaatca ttgttactta tattgattta tgagataggt atcgtcttta tcttattcca
     3601 tattcaaaca ataagtcatc gtttattcat gacatttata tatgcacttg ctatgggtat
     3661 cgtatacttg attattttca tgtaaaggag cgtaactgat gatagaaatt aataatcttt
     3721 caaagcgtta ccgtaacaaa cagattttca atcatttaac tatgtccttt gatagtaatc
     3781 gtttaaccgt attacttggt gataatggtg ctggaaaatc aacattactt cgtatgattg
     3841 ctggtattga aaaagctaat gatggaacta tcaactattt cggcgaaaaa tggaatcaaa
     3901 gacaaataca aaatcacatc ggttatgtgc cacaagacat tgcgttattt gaacacatga
     3961 cagtggctga aaacattaaa ttttttaaat cactttgtaa aaatccaatt aatgatacaa
     4021 ctatcaacga atatttacag caattaaact ttgatgatac gtctgccaaa gtatctacat
     4081 tgtccggtgg aaataaacgt aaaattaata tattagtagg tttactaggt caacctcgaa
     4141 ttctcatttt agatgaaccg acagttggta ttgatttaaa atctagacat gacatccacc
     4201 aactacttaa catcatgaaa tctaaatgtt taattatatt aactacccat catttagatg
     4261 aagtggaagc acttgcagat gatatcaagt taattggcca agatcctttt tatcaacatg
     4321 ttttagagga caagcaatgg gcttatacct attattaaaa cgaaaaaatc ccaagttgcg
     4381 tatgatattg taacttggga tttactgtat tatctacttt gcaagtatga cgttgggtct
     4441 actgcatatt gattaccgat gccaccagac atacgttgga agtgtacgtg aggcgctgtt
     4501 gaattacccg tactacctga atatgcaatt tggtcaccag ctttgacttt atcaccagct
     4561 gaaacagtta aacgattatt atgcatatac cattggtagt tattactgtt cgcttcttta
     4621 atagttactt gattgccgcc accatagtta ctccaacctg cttgtactac tgtaccatca
     4681 gttaatgagt aaactggtga attttcaggc attgcatagt cgacaccgta atgcgcacca
     4741 ccaccgtgat attgtccata tggttgtagt tgtttacgac ttgttaacca gcttgcgtct
     4801 ttcgcatgac cactagctgt cgctttactt gctgatccac cattttgatt agatgtgcca
     4861 ttaggataat tgacctttcc attaccatca tggctgttat acgcttggtt gttgttacta
     4921 tgtgaataat agctcgcgtc tggacctaca tttgattgat aaccatattg attaatatgc
     4981 tgttggcttt gactcgctgt gtagtcattg ttatctcctg ctgttgctgg attcacatat
     5041 gtttggccgc ttccattggc atttgcattt tttggataac agttataaaa ataatgcgta
     5101 tgtccttgag catctacgaa tgtatagcta tattctttat tatcaaacat tgcttgattc
     5161 cagttaccat caggtgtgtg atgataatcc ccattagaat caattgtata ataagtacca
     5221 taagatacgt cttgtgattg tgttgacatt agtgtatgtg cttgttgggt gtttgtcgtt
     5281 tctgctgcat ctgcttgatg cgccattgta aatgtagcga agcccatcgt tgcaatcgct
     5341 gctgctgtta attttttcat gtataaaaca tcctccatta aagttaaagt tagttttcaa
     5401 ttaaactgta ctgcacatac taaaagaatt agacaactga gtaaaggatt taattctcat
     5461 tttccaacta tttaatattc ccgaaatgtt ttactaaact cattacattg tcattacaaa
     5521 atagccatac attgatatta aaatgacatc tctcactgca ttcgcttaag cttttataaa
     5581 ttttcaaaat taacaactaa tcattcgtcc atgttcgcgg ttcaacgcta atgtatgata
     5641 gtaatcatcc atcaaatcat atcaaccaaa ttccattctc aatcgctatt gattgtcatt
     5701 caactttcta atagtgatat gcttctcagg cttaaaaatc gtcatatctt ttctattaat
     5761 taaatcatct gtgagcttta atgctactat ttcattgctg ccataatact taatatataa
     5821 cgttcttgta gttaaattta ttacggtctg atacatcgta tagtgattgt cagcatcatg
     5881 cggacgtaca attccaatcg gtatatttac cgcatctaat aaataaaatg cattcattaa
     5941 atccatttct ttatcattgt tttgagcaat gtttgctttc ataaatgcca ttctcacaaa
     6001 gcgctcagtt gaagtaaatc cacctggcaa tccaaatgta cctgcttcat tgcctaaagg
     6061 ttcaatcgtt acaccttcca ataactttgc tgttgctgga taaggagaaa tattgatata
     6121 ttgtcttaaa ttactataat gccaatctaa gtctggatga tttgttaaga caccaatagg
     6181 gttatctttt ataaccactt ccccctcttt aaatgaaact tcgactgaat gtccagttgc
     6241 atcggaaaca tgataatgca atggcggaac ttcaccgatg tcatttaaat ataccgctac
     6301 aacatgtatt tgggatgctt gttgtttcat atcttcaatg cttgttgtat atcccaaaat
     6361 ccatgtcaca atttcatttt gcgtaatatt catcgcgtcc gctttgtgtg ttgagccata
     6421 tgaactataa cctcggaaat attgtgtcga aatagcaacg ccatgttcat taacaccatc
     6481 accataaata aaaccttcca tatctgttcc tgtaccaata aagccatatt gcgtttggcc
     6541 tgtcgtgcca gtgcgagatg tccaacgata atttctaggc gtcactgctg gcgaaccatc
     6601 taatggataa tcataatcca tcgtgcgtcc aagaagtact tgattattta aagtttgtat
     6661 tgtgaatcct gtgcacattg ttctcactcc tctgtacctt catttacttt aatcactttc
     6721 aaataaagct gtttcactta aacatactat aaaaaatcaa tcatacaagc aattaattga
     6781 tattcattct caataactgt ggtatgatat gtaaggaaat catgacttat gtgtgagtga
     6841 acgatcatct atacatccgt tcacttcatc tcatgacttt ctatatttaa tttttacaag
     6901 gagtgacatc tgtgaataac acacaatctt caccacgcag taatattatt attgcgatta
     6961 tgttgtctgc attaacatat tggttgtttg cacaatcatt tattaatata ggacctctcg
     7021 ttggtcaaac gtatcaaacc tctcctgccg tgttaaattt atctattagt ttaacttcct
     7081 tcgccacagg tatcttcatg gtggctgcag gtgatattgc tgataaaata ggacaactga
     7141 gaatgacata catgggtctc ataatcagta tgtttgcatc tcttctatta attatatcgg
     7201 acatcactgc actgctcatc atcggtagaa ttttacaagg tctatcagca gctatcttgt
     7261 taccttcaac agttggcgtt ttaaataatc aatttaaagg agaacattta agacgagcga
     7321 ttagttatct aatgattagt actgttgggg gcatcggcct agctggtgtt atcggcggtt
     7381 taattgctac aaatttcgga tggcaaatga atttcatcat tagtatagtc attgctttca
     7441 ttgccatatt acttctaaaa ggcacacctg aaaaagtaag tcaacatagc caccgtcatc
     7501 cgttcgatta caaaggtatg tcgattttcg ctgttatgat tggtagcttt acattattgt
     7561 taacacaagg attcgaacaa ggttggttta gtacattctc attcatttgt ctgagcattt
     7621 ttatcatcac tacgctgata ttcatcatca tcgaacgtcg acatgaagta ccttttattg
     7681 atttctcagt attacgcaac cgtccgttca ttggtgcatt tttaaataac tttgttttaa
     7741 atagcggtct aggcgttaca gtggtctttt tcatatatgc tcaaacacac cttggtttat
     7801 cagctgcgca atctggactt gttacattgc catatgccat tgtggcagtt gcgatgattc
     7861 gtttaggtga aaaagcaaca ttacgtttcg gtggcaaatt gatgctcatc attggtccgt
     7921 tgttccctgt catcggcatc actattatta gcatgactca attatcggca tcacaatatg
     7981 tcattgcagt tatcattggt ttcgtcattt gcgcaattgg aaatggttta gtcgcaacac
     8041 ccggccttac aattgcaatt ttcagtatgc caaatgaaaa agttggttta gctacaggat
     8101 tatataaaat gagtggtaca ttaggtggcg cctttggtat agcactaagt actacagttt
     8161 tcagtatgtt acaactaaac tatgcaccaa gtgtagctgc aaccgtaaca tttatcgtca
     8221 gcattgtatt gatgatcctt ggctcattgt ctgcatacat gatcattcca aaaacagtta
     8281 aatcttaaat ataatagaag aattatgttt cgaaatatct ttatcacttt aaaatgatat
     8341 acaagaaatt caagaaaaat aagcgaagaa cgtttttctc tgttcttcgc ttactctaat
     8401 tatattcact atcaattaga caaaatggcc attttcaaat atcacgcgtt gtttctgacc
     8461 ttgaatatat ttattataat tctctttttg aaaatcagtt aacattaatt tagatgtacc
     8521 gtattttaac actttttgca ttgtttctat tctcattttt ctaaataacc atccatcttt
     8581 taacacaata cgattaacag catcatatga taattctact gtttctttaa tttcaaatgt
     8641 cttgaatgaa ataatcgtgc acattaaaaa cgtatcacca aagtaataaa catctaaatc
     8701 atcacgttta tgttgtccaa ctaacaaacg accatattcg aactcttttt ctggatattt
     8761 caattctaaa aaactaataa tctcttcttc ttttaatttg aattgcattt aaaaacatcc
     8821 tctcttaagt tttaacaagc cttaattaaa aatttttt
//
LOCUS       DAHHSC010000098         8851 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.099, whole genome shotgun sequence.
ACCESSION   DAHHSC010000098 DAHHSC010000000
VERSION     DAHHSC010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 8851)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8851)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8851
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.099"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            complement(12..1013)
                     /locus_tag="NBI57_001680"
     CDS             complement(12..1013)
                     /locus_tag="NBI57_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000390648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HCD1822619.1"
                     /translation="MEEFKQHYKGLIDESLTCQDKVELIKKCEKYTDEVIRKDVLPED
                     IVDIHKNYILTLNLTREDVFKTLDVLQEIVKGFGYSYRDYQRLVDKLQVHDKEIDLAS
                     SLQQTMLKTDIPQFDSIQIGVISVAAQKVSGDYFNLIDHNDGTMSFAVADVIGKGIPA
                     ALAMSMIKFGMDSYGHSQLPSDGLKRLNRVVEKNINQNMFVTMFYGLYEEMNHLLYCS
                     SAGHEPGYIYRAEKEEFEEISVRGRVLGISSQTRYQQQEIPIYLDDLIIILTDGVTEA
                     RNSEGTFIDKQKLLEYIKKHKHMHPQDIVQIIYEAILKLQNPNKKDDMTILIIKRVN"
     gene            complement(1362..1724)
                     /locus_tag="NBI57_001681"
     CDS             complement(1362..1724)
                     /locus_tag="NBI57_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HCD1822620.1"
                     /translation="MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVA
                     AITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIRTLDKKRLKEKLTYLSDDKMKEV
                     DNALMISLGLNAVAHQKN"
     gene            complement(1721..1891)
                     /gene="mazE"
                     /locus_tag="NBI57_001682"
     CDS             complement(1721..1891)
                     /gene="mazE"
                     /locus_tag="NBI57_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin MazE"
                     /protein_id="HCD1822621.1"
                     /translation="MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDC
                     NETYLSSNSTNE"
     gene            complement(1976..3124)
                     /gene="alr"
                     /locus_tag="NBI57_001683"
     CDS             complement(1976..3124)
                     /gene="alr"
                     /locus_tag="NBI57_001683"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HCD1822622.1"
                     /translation="MSDKYYRSAYMNVDLNAVASNFKVFSTLHPNKTVMAVVKANAYG
                     LGSVKVARHLMENGATFFAVATLDEAIELRMHGITAKILVLGVLPAKDIDKAIQHRVA
                     LTVPSKQWLKEAIKNISGEQEKKLWLHIKLDTGMGRLGIKDTNTYQEVIEIIQQYEQL
                     VFEGVFTHFACADEPGDMTTEQYQRFKDMVNEAIKPEYIHCQNSAGSLLMDCQFCNAI
                     RPGISLYGYYPSEYVQQKVKVHLKPSVQLIANVVQTKTLQAGESVSYGATYTATDPTT
                     IALLPIGYADGYLRIMQGSFVNVNGHQCEVIGRVCMDQTIVKVPDQVKAGDSVILIDN
                     HRESPQSVEVVAEKQHTINYEVLCNLSRRLPRIYHDGDQRFVTNELLK"
     gene            complement(3190..3549)
                     /locus_tag="NBI57_001684"
     CDS             complement(3190..3549)
                     /locus_tag="NBI57_001684"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HCD1822623.1"
                     /translation="MIHGIGVDLIEIDRIKVLYSKQPKLVERILTKNEQHKFNNFTHE
                     QRKIEFLAGRFATKEAFSKALGTGLGKHVAFNDIDCYNDELGKPKIDYEGFIVHVSIS
                     HTEHYAMSQVVLEKSAF"
     gene            complement(3553..4044)
                     /locus_tag="NBI57_001685"
     CDS             complement(3553..4044)
                     /locus_tag="NBI57_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HCD1822624.1"
                     /translation="MRDYNFMSPHAKKVMRLSAVLFWIPIALVLLIAFNILNWLFWEI
                     LDNNISIVSSVIIILLVAVFTLVIAPVYRYKHCRYAFEKHHLRVRNGILFLDEKVIPY
                     FRIQNIDIDVGPIMRRYRLATLTLYTAGGQAEIELIDRSEARRLKRWLNNERHLENNK
                     NEE"
     gene            complement(4031..5614)
                     /locus_tag="NBI57_001686"
     CDS             complement(4031..5614)
                     /locus_tag="NBI57_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HCD1822625.1"
                     /translation="MSEPQKLHPISYFNGIINAIKQNIVVFFIFIIFQLKDFDYTNPE
                     SYLWIGIVFVFFLISYISQIVGIMNTRYWIEDNYFILTTGIFNKKRKELNIKRIQSVD
                     MTQGVVNQIIGGVDLQIKTPSDGIVLSVISKKQGEYLERYIDQLQTELKTEVAQKSSA
                     EKNDNCNHDIEGNVEDIAEGRLDEVQNTDSMLNQKNKRIPIYQMNFKESLFMAMTSGA
                     IGVTLAALVPIYGAVRELIPWSKLNHEISQWVQIVSISVLIIILLVLIVAYIIGTIIT
                     MIRFYGFSVMLENEQLKIEYGLFNKKKVTVPTKRLQAVVERQSYIRRLFGYTAIHFII
                     TSDFENKKIEDDDNDSGSVPILPFIKRDKAYQIIQNLVPDLSYQQVNEGMPLSGFHRH
                     FLIPSIIVIAMAGIGCYFWNQLLLLFTIITIVIIGLFVIKGYLYVKHAGFKMTEEQIT
                     IQNFELLKRNIYYFKAHHIIGFDTWQHTLLKRNNLNNFSFIVAKGVGAQSINLKYARS
                     EDANKLKSWYLRGENDEGL"
     gene            complement(5607..6086)
                     /locus_tag="NBI57_001687"
     CDS             complement(5607..6086)
                     /locus_tag="NBI57_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1822626.1"
                     /translation="MSDYTKLTKSPKSALLYYYITNGLEFILSVAVYVIFYFIWLRFE
                     WPHYLLYILLVLGALSTLKLFIKPLWQYHCRFYKVDQLSVQYRTSFFIYKEETSRIER
                     LQYLSIKSNPISKVLNLYKVGFMTAGHTIYLPMMSHDDVKIIEARTMSNLRGVESDV"
     gene            complement(6294..6854)
                     /locus_tag="NBI57_001688"
     CDS             complement(6294..6854)
                     /locus_tag="NBI57_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit C"
                     /protein_id="HCD1822627.1"
                     /translation="MNTIRNSICLTIITMVLCGFLFPLAITLIGQIFFYQQANGSLIT
                     YDNRIVGSKLIGQHWTETRYFHGRPSAVDYNMNPEKLYKNGVSSGGSNESNGNTELIA
                     RMKHHVKFGNSNVTIDAATSSGSGLDPHITVENALKQAPRIADARHVSTSRVADLIQH
                     RKQRGVLTNDYVNVLELNIALDKMKD"
     gene            complement(6874..>8851)
                     /gene="kdpB"
                     /locus_tag="NBI57_001689"
     CDS             complement(6874..>8851)
                     /gene="kdpB"
                     /locus_tag="NBI57_001689"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000852430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
                     /protein_id="HCD1822628.1"
                     /translation="KMSFYKLNPKQLIKNPIMFVVEVGMVLTLILICFPDIFGTSYLS
                     RGYLITIFIILLITILFANFSEAFAEGRGKAQADSLRQAQSNLTARLIEENGAYRIVN
                     ATELKAGQNIRVENGETIPADGVVINGLATVDESAITGESAPVIKESGGDFDGVIGGT
                     LVTSDWLEIRVESEAGTSFLDKMIALVEGAERNKTPNEIALFTLLTTLTIIFLVVIVT
                     LYPIASYLHLILPIAMLIALTVCLIPTTIGGLLSAIGIAGMDRVTQFNVLAKSGRAVE
                     VCGDVDVMILDKTGTITYGNRIASEFLPVNQQMLEKLIVAAYMSSIYDDTPEGKSIVR
                     LAKQMYINELPKDIDGTYKPFTAETRMSGIITNEISVFKGAPNSMINLVKQQQGNIPL
                     NIESLCMDVSSKGGTPLIVIENNVMLGVIYLKDVIKDGLVERFTELRKMGIETVMCTG
                     DNALTAATIAKEAGVDRFVAECKPEDKIKVIKDEQAKGHIVAMTGDGTNDAPALAQAN
                     IGLAMNSGTISAKEAANLIDLDSNPTKLIEVVKIGKQLLMTRGALTTFSLANDVAKYF
                     AILPALMMSTIPEMTSLNIMHLSSPKSAIISALIFNALIIVALIPIAMKGVKVKGYSI
                     DRIFINNMLIYGLGGLIVPFLGIKLIDMIVQFFV"
BASE COUNT         2795 a         1717 c         1209 g         3130 t
ORIGIN      
        1 tcttttttaa attaatttac tctttttata atcaaaatag tcatatcatc ttttttattt
       61 gggttttgaa gctttaaaat tgcttcatag ataatttgaa caatatcttg tgggtgcata
      121 tgtttatgtt ttttaatata ttctaaaagt ttttgtttat ctataaaggt accttcacta
      181 tttctagctt cagtcacacc atccgttaaa atgataatta aatcatcaag gtatatagga
      241 atttcttgtt gttgatatcg tgtttgtgaa ctgattccta acactctacc tctaactgaa
      301 atttcttcaa attcttcttt ttcagcgcga taaatatatc caggctcatg accagctgaa
      361 ctacaataca ataaatggtt catttcttca tataaaccat aaaacattgt gacgaacata
      421 ttttgattaa tattcttttc aacaacacga tttaaacgtt ttaagccatc actcggtaat
      481 tgtgagtgtc cataagaatc catgccaaac tttatcatac tcattgctaa agcagctggt
      541 atacctttcc caatgacatc tgcaacagca aagctcattg tgccatcgtt atggtcaatt
      601 aaattaaaat aatctccact tactttttgt gctgccactg aaataacgcc aatttgaata
      661 ctatcaaatt gtggaatatc tgttttaagc attgtttgtt gtaagctaga agctaagtct
      721 atctctttat cgtgaacttg aagtttatct accaatcttt gataatctcg ataactataa
      781 ccaaagcctt taacgatttc ttgtaagaca tctaatgtct tgaacacatc ttcacgcgtt
      841 aagtttaacg tcaatatata gtttttgtga atatcgacaa tgtcttcagg caagacgtcc
      901 ttacgaatca cttcgtcagt gtatttctca cactttttta tcaattctac tttatcttgg
      961 cacgttaaac tttcatcaat taaaccctta taatgttgct taaattcttc cacgattagt
     1021 tgcctcctca cttcccttac aaagcaaatg cttaagcaag gataattatg attcacttac
     1081 taacatttat ttaattgcac ttatcaattt tgtcgtttgc aattttgctg tagatgatac
     1141 ttttaagcta aaaaaacttt aggatggaat acctaaagcc taaaaaactt aagacatcct
     1201 tttataataa aaaatatgaa tatcataact ggtatttgct aaaatctatg tttataagaa
     1261 aattcttcgt acagaattta gaaatagtta taattattat aaagttattg tcttttatat
     1321 caatatttta tttatctctg aaaaatacat ataatagacg cctaattttt ctggtgagct
     1381 actgcattca gccctaaact aatcattagt gcattatcta cttctttcat tttatcatcg
     1441 gataagtacg tcagtttttc tttcaatcgt tttttatcaa gtgtacgaat ttgttctaat
     1501 aatataactg agtctttatc caatttatac tttttctttt caatctctac atgtgtcggt
     1561 attttcgctt tattaatcct accagttatt gccgcaacaa taactgtagg actatattta
     1621 ttaccagtat cattttgaat tatgactaca ggtctgactc ccccttgttc agatccctgt
     1681 actggtgata aatctgctaa ataaacatct cctcgtctaa tcattcattc gttgaattag
     1741 aagataaata tgtttcgttg caatcgcatg cttcacactc tatcggaaaa gcttcgttcg
     1801 ctagggagag atttaaatca gccatttgtg aatatccttc ttttaaagat tgttctaagc
     1861 tatgacttct attttgacta aaagataaca tatgagaacc tccaattgat tccaaaagtt
     1921 attaaatgac ctttaattac tctaatgata acaaatttta tatgcactga ccatattatt
     1981 ttaacaattc atttgttaca aaacgttgat caccatcatg atagattcgc ggcaaacgtc
     2041 tcgacaagtt acaaagcact tcataattaa tagtatgttg cttttcagct accacctcta
     2101 ccgactgtgg actttctcta tgattatcta ttaaaatcac cgaatctcca gctttaactt
     2161 gatctggcac tttaacaatt gtctgatcca tacatacgcg accaataact tcgcattgat
     2221 gaccatttac atttacaaag ctaccttgca ttatgcgtaa atagccatct gcatatccaa
     2281 taggtaacaa agctattgta gttgggtcag tagctgtata agttgcacca taacttacag
     2341 actcacccgc ttgtagcgtc tttgtttgaa ctacattagc aattaattgc acacttggtt
     2401 taaggtgtac tttaactttt tgctgtacat actctgatgg ataatatcca taaagggaaa
     2461 ttcctggtct tattgcatta cagaattggc aatccattaa tagagagcct gctgagttct
     2521 gacaatgtat atattcaggt ttaattgctt cattgaccat atctttaaaa cgttgatatt
     2581 gttcagttgt catatctcct ggttcgtcag cacaggcaaa gtgtgtaaac acgccttcaa
     2641 atacaagttg ctcatattgt tgaatgattt caatcacttc ttgatacgta ttagtatctt
     2701 taatacctaa acgtcccatt cctgtatcta atttaatgtg caaccataac tttttctctt
     2761 gctcaccaga aatgttttta attgcttctt tcaaccactg tttagacgga accgttaagg
     2821 caactctgtg ttgtatcgct ttatcaatat ctttagctgg taacacacct aagactaaaa
     2881 ttttagcagt aatcccatgc attctaagtt ctatcgcttc atctaacgtt gctacagcaa
     2941 aaaatgtggc gccattttcc attaaatgac gtgctacttt aacactacct agtccatagg
     3001 cattggcttt aacgacagcc atcactgttt tatttggatg caatgtactg aatactttga
     3061 aatttgatgc aacagcgttt aaatctacat tcatatacgc agatctataa tatttatccg
     3121 acatattact tcctcctgta attcccacac gttttaaaac tagatcttaa ttatcattgt
     3181 ataacaaatt taaaatgctg acttttctaa aacaacttgg ctcatcgcat aatgctcagt
     3241 gtgtgagata ctaacatgta cgatgaaccc ttcgtaatca atctttggtt tgccaagttc
     3301 gtcgttgtaa cagtctatat cgttaaaagc tacatgtttt cctaagcctg tgcctaatgc
     3361 tttactgaac gcttcttttg tagcaaacct gccagctaaa aattcaattt tacgttgctc
     3421 atgtgtgaaa ttgttgaatt tgtgctgttc atttttagtt aaaatccgct caaccaattt
     3481 tggctgctta ctatataaca cttttattcg atcgatttcg attaaatcta caccaattcc
     3541 atgtatcatt gattattcct catttttatt gttttctaaa tgtctctcgt tatttaacca
     3601 tcgtttcaat ctacgtgctt cacttctatc tattaattca atttcagctt gaccacccgc
     3661 tgtataaagg gtcaaagttg ctaatcgata gcgtctcatt ataggaccaa catcgatatc
     3721 aatattttga atacgaaaat atggtattac tttttcatcc aaaaataaaa tgccgtttcg
     3781 tacacgcaaa tggtgttttt caaatgcata tctgcagtgc ttatatcgat atactggcgc
     3841 tatgacaagc gtgaaaacag caacaagtaa tattataatc acactactaa caatggatat
     3901 attgttatct aatatctccc aaaatagcca gttcaaaata ttaaatgcga ttaaaagtac
     3961 aagcgctatg ggtatccaaa acagcacagc acttaacctc atcacttttt tagcgtgtgg
     4021 tgacataaaa ttataatccc tcatcatttt cacctcttaa ataccatgat ttcaatttat
     4081 ttgcatcttc acttcttgcg tattttaggt taatcgattg ggctcccaca cctttagcaa
     4141 caataaagct aaaattattt aaattgttgc gtttaagtaa tgtatgttgc caagtgtcaa
     4201 atcctataat gtgatgcgct ttaaaataat aaatatttcg tttcaatagc tcgaaattct
     4261 ggatagtaat ttgttcttct gtcattttaa aacccgcatg tttgacataa agatatcctt
     4321 tgatcacaaa taaaccaata atgactattg ttataatcgt aaataacaat aataattgat
     4381 tccaaaaata acagccaata cctgccatag ctatgacaat aatactaggt attaaaaagt
     4441 gtctgtggaa acctgacaaa ggcatacctt cattaacttg ttgataagat aaatctggta
     4501 ctaaattctg gataatttga tatgctttgt ctcgtttaat aaacggcaat atcggcacac
     4561 tacctgaatc attgtcatca tcttcgattt ttttattttc aaaatcactt gtgatgataa
     4621 aatgtattgc tgtgtagcca aataatcttc gtatatatga ctgacgttca acaacagctt
     4681 gcaatcgttt cgttggtaca gttactttct tcttgttaaa gagaccatat tcaattttaa
     4741 gttgctcatt ttcaagcatc accgaaaagc cataaaatct tatcattgtt ataatcgttc
     4801 caataatata tgccactatt aatactagta aaatgatgat taatactgaa atacttacaa
     4861 tttgaaccca ttgactaatt tcatgattta gcttcgacca tgggatcaac tctcttacag
     4921 ccccgtaaat cggtactaaa gctgctaacg ttacaccaat ggcgccactg gtcattgcca
     4981 taaatagtga ttctttaaaa ttcatctgat atataggaat gcgtttattt ttctgattaa
     5041 gcatactatc agtgttctgc acttcatcta agcgaccttc tgcgatgtct tccacattac
     5101 cttcaatgtc atgattacag ttgtcattct tctcagcact agacttttgc gccacttctg
     5161 tcttcaactc tgtttgcaat tgatcaatat atcgttcaag atattcacct tgttttttcg
     5221 aaataacact taagacaata ccatcacttg gtgttttgat ttgtaaatcc acaccaccaa
     5281 taatttgatt gacgacccct tgtgtcatat caacggactg aatacgtttt atatttaatt
     5341 ctttacgctt tttattaaaa atacctgttg ttaaaatgaa ataattatcc tcaatccaat
     5401 atcgcgtgtt cataattccg acaatttgag aaatgtatga tattaaaaag aatacaaata
     5461 caatacctat ccataaatat gattcgggat tcgtataatc aaaatctttc aattgaaaga
     5521 taatgaaaat aaaaaagacg actatgtttt gtttgatagc attgattatg ccattaaaat
     5581 atgaaatcgg atgtaatttt tgaggttcag acatcacttt caacccctct caaattcgac
     5641 atagttctcg cttcgattat tttaacatcg tcatgagaca tcatcggtaa ataaatagta
     5701 tgacctgcag tcataaatcc aactttatac aaattaagca ctttactaat tggattagat
     5761 ttaatcgaca agtattgtaa acgttcaatt cgactcgttt cttctttata tataaaaaat
     5821 gatgtacgat attgtacact tagttgatca actttataaa agcgacaatg atattgccat
     5881 aaaggcttaa taaataattt taatgtactc agagcaccta aaaccaacaa aatataaagt
     5941 aagtaatgtg gccattcaaa tcttaaccat ataaaataaa aaatgacata cacagctaca
     6001 ctcaatataa attctaagcc attcgtaatg tagtaataca acaatgctga cttaggactc
     6061 ttagtcaact tagtataatc tgacataacc cctctcccca aataaaaaat tatacggatt
     6121 tataatctat ttcattttat ttttatatga tgataattat agcatatgga atatttcatg
     6181 ctaatttatt cttcctaaag gtacatctaa aaatttaatt aagcagaaag tgcttgaatt
     6241 gctaaaaaga caccatgtta taattttatc aacatgatgc ctttcatcta taatcaatct
     6301 ttcatcttat caagagcgat atttagttca agcacattca cataatcatt tgttaacaca
     6361 ccacgctgct tacgatgttg aatcaagtcg gccactcttg aagtagatac atgacgagca
     6421 tcagcaatac gaggtgcttg cttcaatgca ttttcgaccg taatatgcgg atctaagccc
     6481 gacccagaac ttgttgcagc atctattgtt acatttgaat tcccaaattt aacatgatgt
     6541 ttcatgcgtg ctattaattc ggtgtttcca ttcgattcat tacttccacc tgaagatacg
     6601 ccgtttttat ataatttttc aggattcata ttataatcaa ctgcactcgg tctcccgtga
     6661 aaatatcgtg tctctgtcca gtgctgtcca atcaattttg atccaactat acgattgtca
     6721 tacgtaatta aactgccatt tgcttgttga taaaaaaata tttgaccaat taacgtgata
     6781 gctaacggga ataaaaatcc acataatacc atagttatta tcgttaaaca aatactattt
     6841 cttatcgtat tcatggtaca ggctccttcc tctttacaca aaaaattgta caatcatatc
     6901 tattaattta atgcctaaaa acgggacgat taatccacct aatccataaa tcaacatatt
     6961 atttataaag attctatcaa tgctgtaacc ctttactttt acacctttca tggcaattgg
     7021 aattaaggca acaatgatta atgcattgaa tatcaaagct gatataattg cagatttagg
     7081 tgacgataaa tgcataatat ttagtgaagt catttctgga attgttgaca tcattagtgc
     7141 aggtaaaatt gcaaagtatt ttgctacgtc attagccaaa ctaaatgtcg ttaatgcacc
     7201 tctcgtcatt aataattgtt tgcctatttt tacaacctct attaactttg taggattcga
     7261 atctaaatca attagattag ctgcctcttt agcactaatt gtccctgagt tcatagctaa
     7321 tcctatattc gcttgtgcta gcgcaggtgc atcatttgta ccatctcctg tcatcgcaac
     7381 aatatggcct ttcgcttgtt catctttgat gactttaatt ttatcttcgg gtttacactc
     7441 tgcaacaaat ctatcaaccc cggcttcttt tgcaattgta gctgctgtta aagcattatc
     7501 acctgtacac ataactgttt caatccccat ttttctcaat tcagtaaatc gttctacaag
     7561 accatcttta atcacatctt ttaaataaat cacgccaagc atgacattgt tttcaatgac
     7621 tattaatggt gtgccacctt tactcgatac atccatacag agagactcaa tgttaagagg
     7681 aatattgcct tgttgttgtt tgacaagatt tatcatacta ttaggtgcac ctttgaatac
     7741 cgatatttca tttgtaatga ttccgctcat tctagtttca gctgtaaaag gcttatatgt
     7801 gccatcaatg tctttaggca gctcatttat atacatctgc ttcgctaatc gtacaatact
     7861 ttttccttct ggcgtatcat cgtagattga tgacatataa gcagcgacta tcaatttttc
     7921 cagcatttgt tgattcactg gtaaaaattc actagcgatt cgattgccat aagtgattgt
     7981 ccctgtcttg tctaaaatca ttacatcgac atctccacat acttctacag cacgcccact
     8041 tttcgctaat acattgaatt gagtaacacg atccatgcct gcaataccaa tcgccgataa
     8101 caaaccaccg attgtcgttg gtattaaaca tactgttaac gcaatgagca tcgcaatagg
     8161 taaaattaaa tgcaggtaag atgctattgg atataacgtt acaataacga ctaaaaatat
     8221 aattgttaac gttgttaata atgtaaaaag tgcaatttca tttggtgttt tatttctttc
     8281 cgccccttca actaaggcaa tcattttatc taaaaaagat gtacccgctt cactctcaac
     8341 acgtatttct aaccaatcag atgttacaag tgtaccgcca atgactccat caaaatcgcc
     8401 acctgattct tttatcacag gtgcagactc accagtaatt gcagattcat caacggttgc
     8461 taatccattt attacaacgc catcagcagg gattgtttct ccattttcta cccgaatatt
     8521 ttgtccggct tttaactctg tggcgttcac tatccgatac gcaccatttt cttctatcaa
     8581 tcgagcagtt aaatttgatt gtgcttgtct taaactatca gcttgcgctt ttccacgacc
     8641 ttcagcaaag gcttctgaaa aattagcaaa caatatagtt attaataata tgataaaaat
     8701 tgtaatcaaa taacctcgcg atagatagct agttccaaat atgtcaggaa aacatattaa
     8761 tatcaacgtt aaaaccattc caacctcaac gacaaacatt atcggatttt ttattaattg
     8821 tttaggattc agcttataaa aactcatttt c
//
LOCUS       DAHHSC010000099         8771 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.100, whole genome shotgun sequence.
ACCESSION   DAHHSC010000099 DAHHSC010000000
VERSION     DAHHSC010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 8771)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8771)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8771
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.100"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            135..1325
                     /locus_tag="NBI57_001690"
     CDS             135..1325
                     /locus_tag="NBI57_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine--oxo-acid transaminase"
                     /protein_id="HCD1822629.1"
                     /translation="MTKSEKIIELTNHYGAHNYLPLPIVISEAEGVWVKDPEGNKYMD
                     MLSAYSAVNQGHRHPKIIQALKDQADKVTLVSRAFHSDNLGEWYEKICKLAGKDKALP
                     MNTGAEAVETALKAARRWAYDVKGIEPNKAEIIAFNGNFHGRTMAPVSLSSEAEYQRG
                     YGPLLDGFRKVDFGDVDALKAAINENTAAVLVEPIQGEAGINIPPEGYLKAIRELCDE
                     HNVLFIADEIQAGLGRSGKLFATDWDNVKPDVYILGKALGGGVFPISVVLADKEVLDV
                     FTPGSHGSTFGGNPLACAASIAALDVIVDEDLPGRSLELGDYFKEQLKQIDHPSIKEV
                     RGRGLFIGVELNESARPYCEALKEEGLLCKETHDTVIRFAPPLIITKEELDLALEKIR
                     HVFQ"
     gene            1434..2678
                     /locus_tag="NBI57_001691"
     CDS             1434..2678
                     /locus_tag="NBI57_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HCD1822630.1"
                     /translation="MTENNNLVTSTQGIIKEALHKLGFDEGMYDLIKEPLRMLQVRIP
                     VRMDDGTVKTFTGYRAQHNDAVGPTKGGVRFHPDVDEEEVKALSMWMTLKCGIVNLPY
                     GGGKGGIVCDPRQMSIHEVERLSRGYVRAISQFVGPNKDIPAPDVFTNSQIMAWMMDE
                     YSALDKFNSPGFITGKPIVLGGSHGRDRSTALGVVIAIEQAAKRRNMQIEGAKVVIQG
                     FGNAGSFLAKFLYDLGAKIVGISDAYGALHDPNGLDIDYLLDRRDSFGTVTNLFEETI
                     SNKELFELDCDILVPAAISNQITEDNAHDIKASIVVEAANGPTTPEATRILTERGILL
                     VPDVLASAGGVTVSYFEWVQNNQGYYWSEEEVNEKLREKLEAAFDTIYELSQNRKIDM
                     RLAAYIIGIKRTAEAARYRGWA"
     gene            complement(3205..4134)
                     /locus_tag="NBI57_001692"
     CDS             complement(3205..4134)
                     /locus_tag="NBI57_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HCD1822631.1"
                     /translation="MTNSSKSFTKFMAASAVFTMGFLSVPTAGAEQTNQIANKPQAIQ
                     WHTNLTNERFTTIAHRGASGYAPEHTFQAYDKSHNELKASYIEIDLQRTKDGHLVAMH
                     DETVNRTTNGHGKVEDYTLDELKQLDAGSWFNKKYPKYARASYKNAKVPTLDEILERY
                     GPNANYYIETKSPDVYPGMEEQLLASLKKHHLLNNNKLKNGHVMIQSFSDESLKKIHR
                     QNKHVPLVKLVDKGELQQFNDQRLKEIRSYAIGLGPDYTDLTEQNTHHLKDLGFIVHP
                     YTVNEKADMLRLNKYGVDGVFTNFADKYKEVIK"
     gene            complement(4376..5755)
                     /gene="argH"
                     /locus_tag="NBI57_001693"
     CDS             complement(4376..5755)
                     /gene="argH"
                     /locus_tag="NBI57_001693"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HCD1822632.1"
                     /translation="MSNKAWGGRFEVQPEEWVDDFNASITFDQTLIDQDIEGSIAHAT
                     MLANQGIISQQDSEQIIQGLKSIQHDYHQDQIQFSASLEDIHLNIEHELIKRIGDAGG
                     KLHTGRSRNDQVATDMHLYTKKQVQDIIALIKSLQSVIVDIASNNVDTIMPGYTHLQR
                     AQPISFAHHIMTYFWMLQRDQQRFEDSLKRIDINPLGAAALSGTTYPIDRHETTALLN
                     FGSLYENSLDAVSDRDYIIETLHNISLTMVHLSRFAEEIIFWSTDEAKFITLSDAFST
                     GSSIMPQKKNPDMAELIRGKVGRTTGHLMSMLMTLKGLPLAYNKDMQEDKEGLFDAVH
                     TIKGSLRIFEGMIQTMTINKERLNQTVKEDFSNATELADYLVTKNIPFRTAHEIVGKI
                     VLECIQQGHYLLDVPLATYQQHHSSIDADIYDYLQPENCLKRRQSYGSTGQSSVKQQL
                     DVAKQLLSQ"
     gene            complement(5745..6950)
                     /locus_tag="NBI57_001694"
     CDS             complement(5745..6950)
                     /locus_tag="NBI57_001694"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HCD1822633.1"
                     /translation="MKEKIVLAYSGGLDTSVAVQWLIDKGYDVVACCLDVGEGKDLDI
                     VYKKALDMGAVECHIIDATKEFSDEYVSYAIKGNLMYENAYPLVSALSRPLIAKKLVE
                     IAEKTNSVGIAHGCTGKGNDQVRFEVAIKALNPSLKAFAPVREWAWSREEEIDYAIKH
                     NIPVSINHDSPYSIDQNLWGRANECGILEDPYAAPPEDAFDLTNALEETPDTADEIIL
                     TFDKGIPVQIDGKTYELDDLILTLNALAGKHGIGRIDHVENRLVGIKSREIYEAPAAE
                     VILKAHKALETITLTKDVAHFKPIIEKQFAEQLYNGLWFSPLTDSLKLFIDSTQQYVS
                     GDVRIKLFKGNAIVNGRKSPYTLYDEKLATYTKEDAFNQDAAVGFIDIYGLPTQVNAM
                     LHGGYSNEQ"
     gene            7301..8632
                     /locus_tag="NBI57_001695"
     CDS             7301..8632
                     /locus_tag="NBI57_001695"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HCD1822634.1"
                     /translation="MTHIQLDFSKTLEFFGEHELKQQQEIVKSIHKTIHEGTGAGSDF
                     LGWVDLPVDYDKEEFSRIVEASKRIKENSDVLVVIGIGGSYLGARAAIEMLTSSFRNS
                     NEYPEIVFVGNHLSSTYTKELVDYLADKDFSVNVISKSGTTTEPAVAFRLFKQLVEER
                     YGKEEAQKRIFATTDKEKGALKQLATNEGYETFIVPDDVGGRYSVLTAVGLLPIATAG
                     INIEAMMIGAAKAREELSSDKLEDNIAYQYATIRNILYAKGYTTEMLINYEPSMQYFN
                     EWWKQLFGESEGKDFKGIYPSSANYTTDLHSLGQYVQEGRRFLFETVVKVNHPKYDIT
                     IEKDSDDLDGLNYLAGKTIDEVNTKAFEGTLLAHTDGGVPNMVVNIPQLDEETFGYVV
                     YFFELACAMSGYQLGVNPFNQPGVEAYKQNMFALLGKPGFEDLKKELEERL"
BASE COUNT         2897 a         1398 c         1592 g         2884 t
ORIGIN      
        1 aatagacaaa tttaatgaaa cttagtgatt atttcatctt ttttggtgga aaagtatctg
       61 ctttgaaaac taatgtaatt atttggataa tgtttcatgt ataatagtta taaatgatga
      121 aaaggggaga tatgatgact aaatctgaaa aaattattga gttaacaaat cattacggag
      181 cacataatta tttaccattg ccaattgtca tttcagaagc tgaaggggta tgggttaaag
      241 atcctgaagg caataaatat atggatatgt tatctgcata ttccgctgtt aaccaaggtc
      301 atagacatcc gaaaattatt caagcattaa aagatcaagc tgataaagtg actctagttt
      361 cacgtgcttt tcatagtgat aacttaggtg aatggtacga aaaaatttgt aaactggcag
      421 gtaaagataa agctttacca atgaatacag gtgctgaagc agtagaaaca gctttgaaag
      481 cagcacgacg ctgggcatac gatgttaaag gaattgagcc aaataaagca gaaatcattg
      541 catttaatgg taacttccat ggtcgaacaa tggcgccagt ttcattatct tcagaagcag
      601 aataccaacg tggttatggt ccgttattag atggatttag aaaagttgat tttggagatg
      661 tagatgcatt gaaagctgca attaatgaaa atactgcagc agttttagta gaaccaattc
      721 aaggtgaagc gggtataaat ataccgccag aaggatattt gaaagcaatt agagaattat
      781 gtgatgaaca taatgtctta tttattgctg acgaaatcca agcaggatta ggtcgttcgg
      841 gtaaattatt tgctacggat tgggataatg taaaacctga tgtctatatt ttaggtaaag
      901 cactaggtgg tggtgtcttc ccaatttctg ttgtattagc agataaagaa gtattagatg
      961 tctttacacc tggctcacat ggttcaacat ttggtggtaa tccacttgct tgtgctgcat
     1021 caattgctgc attagatgtt atcgttgatg aggatttacc agggcgctct ttagaattag
     1081 gagattattt taaagaacaa ttaaagcaaa ttgatcatcc atcaattaaa gaagtccgtg
     1141 gacgtggttt gtttataggt gtggaactta atgaaagtgc tagaccatat tgtgaagctt
     1201 tgaaagaaga aggcttatta tgtaaagaaa cgcatgatac tgtcattcgt tttgcaccac
     1261 cattaattat tactaaagaa gaattggacc ttgcacttga aaaaataaga catgtatttc
     1321 aataatgtca aaaagttgta acaatcagtt tgtaagggct ttaaaaagga atacgttatg
     1381 ttacaatatg tttgaaagcg aaatcattag gtaaagaaga ggcgaaaagg atcatgactg
     1441 agaacaataa tttagtaact tctactcaag gaattattaa agaagcattg cataaattgg
     1501 gatttgacga aggaatgtac gatttaatta aagaaccttt aagaatgtta caagtgcgta
     1561 tccctgtacg aatggatgat ggcacagtta aaacattcac aggttaccgt gcgcaacata
     1621 atgatgctgt tggaccaaca aaagggggcg tgcgtttcca cccagatgtt gatgaagaag
     1681 aagtaaaagc attatcaatg tggatgactt tgaaatgtgg cattgtaaac ttaccatacg
     1741 gtggtggtaa gggtggtatc gtttgtgatc cacgtcaaat gagcattcat gaagttgaac
     1801 gtttatcacg cggatatgta agagcaattt cacaattcgt aggtccgaac aaagatattc
     1861 cagcaccaga tgtatttaca aactcacaaa ttatggcttg gatgatggat gaatatagtg
     1921 cattagataa atttaattca ccaggtttca tcacaggtaa accaattgta ttgggtggtt
     1981 ctcatggacg cgacagatca actgcactag gtgtagttat tgcaattgaa caagctgcaa
     2041 aacgtcgtaa tatgcaaatt gaaggtgcca aggttgttat tcaaggtttc ggtaatgccg
     2101 gaagtttctt agctaaattc ttatatgatt taggtgcaaa aattgtaggt atctctgatg
     2161 cttacggtgc attacacgat ccaaatggct tagatataga ttatttatta gaccgtcgtg
     2221 atagttttgg tacggtaaca aatttatttg aagaaacaat ctcaaataaa gaattgtttg
     2281 aattagattg tgacatttta gtaccagcgg ctatttcaaa ccaaattaca gaagacaatg
     2341 cacatgatat taaagctagt atcgttgttg aagctgctaa tggacctaca acaccagaag
     2401 caacacgtat tttaactgaa cgtggtatat tattagttcc agacgtatta gcaagtgctg
     2461 gtggtgtaac ggtttcttac ttcgaatggg tacaaaataa tcaaggttat tattggtctg
     2521 aagaagaagt aaatgaaaag ctacgtgaaa aattagaagc ggcatttgac acgatttacg
     2581 aattgtctca aaatcgaaaa atagatatga gacttgcagc atatatcata ggtattaaac
     2641 gtacagcaga agcagctaga tatcgtggtt gggcataatt aattatcata tgtgatttaa
     2701 cgagcttggg acagaaaaca aagccctaag ctcgttaatt ttattttagc agcagttgac
     2761 tgtaaaacaa tgcccgtgta acacgctctt ttcaaaaata gtcggggccc caacacagag
     2821 gctggtggaa agtcagctta caatagtgtg caagttggct ggcccccaac actgagaatt
     2881 tttaattaaa ccaggctggg acataaatca atgttctatg ctctacgaag ttatattggc
     2941 agtagttgac tgaacgaaaa tgcgcttgta acaagctttt ttcaatttta gtcaggggcc
     3001 ccaacacaga gaatttcgaa aagaaattct acagacaatg caagttggcg aggccccaac
     3061 actgagaatt tctaattaaa cgctcaacta tatatagttg atcaaaaaga cagcagtagg
     3121 ataatttcaa tttggaaata tcttactgct gttttttata gtaatagcta tatttttgta
     3181 tttatgtttt ctagtttaac aatactactt aatgacttct ttatatttat cagcgaaatt
     3241 tgtaaagaca ccatcaacgc catatttatt taatcgtaac atatcagctt tttcattcac
     3301 tgtataagga tgtactataa atcctaagtc ttttaaatga tgggtatttt gttcagttaa
     3361 atctgtataa tcaggaccta atccaatcgc ataagagcgt atctctttta agcgttggtc
     3421 gttaaattgt tgtagttcac ctttatcaac taattttact aatggcacat gcttattttg
     3481 acgatgaatt ttctttaaac tttcgtcaga aaatgattga atcattacat gtccattttt
     3541 taatttattg ttatttaaaa ggtgatgctt tttcaatgaa gctaataatt gttcttccat
     3601 tcctgggtat acatcaggtg actttgtttc aatataatag tttgcattcg ggccataacg
     3661 ttctaaaatt tcatctaaag tgggtacttt agcattttta taacttgctc ttgcgtattt
     3721 tggatatttt ttattaaacc aacttcctgc atctaactgt tttaattcat caagggtata
     3781 atcctcaact ttaccgtgtc catttgttgt acggttaaca gtttcatcat gcatagcaac
     3841 taaatgtcca tctttggtac gttgtaaatc aatttcgata taagatgctt ttaactcatt
     3901 atgactctta tcatatgctt gaaacgtatg ctctggtgca tagccacttg cgccacgatg
     3961 tgcgatagta gtgaatcgct cattcgttaa atttgtatgc cattgaatag cctgaggttt
     4021 atttgcaatt tgatttgttt gttcagcgcc agcagtaggt actgataaaa atcccatagt
     4081 aaaaacagca gaagcagcca taaatttagt gaagcttttc gaagagttag tcatagtctt
     4141 tatcctttca tctctttatt tgtagtctaa caattttaaa atactacaaa aacctgcttg
     4201 tgaaaacgct ttacacattt cttttacaat ttataacatt tctgtaatat aaatttaaaa
     4261 gaattatcaa acattttagg aatcttaaaa atgtgatgtt caacaagacg aagagagcat
     4321 taaaataccc acaatgtaat tttaatagac attgtgggta ggtagattaa cgtatttatt
     4381 gtgatagtaa ttgtttagca acatcaagtt gttgtttgac cgatgattgc cccgttgacc
     4441 cgtaactttg acgtcgtttc aagcaatttt caggctgcaa ataatcgtaa atatcggcat
     4501 cgatactaga atgatgttgt tgatatgttg ctaaaggaac atctaataaa taatgacctt
     4561 gttgtataca ttctaagacg atttttccta caatttcatg tgcagttcta aatggaatat
     4621 ttttagttac taaataatct gctagttccg ttgcatttga aaaatcttct ttaacagttt
     4681 gattgagtcg ttctttatta attgtcatcg tttgaatcat accttcgaaa atacgtaaag
     4741 aacctttaat tgtatggaca gcatcgaata aaccttcttt atcttcctgc atatctttgt
     4801 tatatgctag aggtaatcct tttaaagtca taagcatgct cattaaatga ccagtcgttc
     4861 gaccaacttt acctctaatt aattctgcca tatcaggatt tttcttttgt ggcataatag
     4921 atgagccagt tgaaaatgca tctgataatg taatgaattt agcttcgtct gtggaccaga
     4981 aaataatttc ctctgcaaag cgtgataagt gaaccatcgt taaagaaata ttatgcaatg
     5041 tttcaataat atagtctctg tcactaacag catctaggct attctcatag agactgccaa
     5101 agttcaacaa tgctgttgtc tcgtgtctat cgatagggta tgtggtacca cttaaggctg
     5161 ctgcacctaa aggattaata tcgattcgtt ttaaactatc ttcaaatcgt tgttggtctc
     5221 gttgtaacat ccaaaaataa gtcataatat gatgtgcaaa tgaaattggc tgtgcacgct
     5281 gtaaatgagt ataaccaggc ataattgtat caacattatt ggaagcgatg tctacaatta
     5341 cactttgtaa cgacttaatt aatgcgatga tatcttgcac ttgtttctta gtgtacaagt
     5401 gcatatctgt tgcaacttga tcgtttctac tgcgtccagt atgcaactta ccaccagcat
     5461 caccaatacg tttaattaat tcatgttcaa tatttaaatg aatatcttct aatgatgcac
     5521 taaattgaat ttgatcttga tgataatcat gttgaataga ttttagtcct tgtataattt
     5581 gttcgctgtc ttgttgacta ataatgcctt gattcgcaag catagttgca tgcgcaatgc
     5641 tgccttcgat atcttgatct atgagcgttt ggtcaaaagt aatggatgcg ttaaagtcgt
     5701 caacccactc ttcaggttgt acttcaaatc taccgcccca agctttattg ctcattgcta
     5761 tagcctccat gcaacatcgc atttacttga gtaggtaaac catagatatc gataaagcca
     5821 acagcagcgt cttgattaaa tgcatcttct tttgtataag ttgctaattt ttcatcatat
     5881 aatgtgtaag gtgattttct accattcacg atggcattac ctttgaataa tttaattctg
     5941 acatcaccac ttacgtattg ctgagtacta tcaataaata atttcaagct atcagttaaa
     6001 ggtgagaacc aaagtccatt gtatagttgt tcagcaaatt gcttctcaat gattggttta
     6061 aagtgtgcga catctttagt taacgtaatc gtttctaatg ctttatgcgc ttttaaaata
     6121 acttctgcag caggtgcctc ataaatttct cttgatttga tacctacaag tctattttct
     6181 acatggtcaa ttcttccgat accatgctta ccagctaatg cattcaacgt taaaattaaa
     6241 tcgtctaatt catatgtttt gccatcaatt tgaactggga tgcctttatc aaacgttaaa
     6301 atgatttcat cagcagtatc tggtgtttct tctaaagcat ttgttagatc gaacgcatcc
     6361 tctggtggcg cagcataagg atcttctaaa ataccacatt cattcgctct gccccataga
     6421 ttttgatcga tagaataagg tgaatcatgg ttgattgata cagggatatt atgtttaatt
     6481 gcataatcga tttcttcttc acgactccat gcccactcac gtacaggtgc gaatgctttc
     6541 aatgatgggt ttaatgcttt aatggcaact tcgaaacgta cttggtcatt ccctttacca
     6601 gtacaaccat gtgcaatacc tactgaattt gttttctcag caatctctac taatttttta
     6661 gcgattaatg gtcttgataa agctgaaact aatggatatg cattttcata cattaaattt
     6721 cctttgattg cataacttac atactcatca ctaaattctt ttgttgcatc aataatatga
     6781 cattcaactg ctcccatatc taaagctttt ttataaacga tgtctaaatc tttaccttca
     6841 ccaacatcta ggcaacaagc tacaacgtcg tatcctttgt cgataagcca ttgaacggcc
     6901 acacttgtat ctagtcctcc tgaatatgct aaaacaattt tctctttcat aaaattcacc
     6961 tcattgtagt tatctgaata tttcgtatga acacatgata gcaaactttt aatgaaaata
     7021 aaaccattat tttacatatt tattcataaa atgcgataga gatagttaga gttgtaagta
     7081 taaatgctta tttatcaatg tttaaaagta ttttaaaaaa catgcaaatt gtataaaaaa
     7141 tgtttgagtg tttgacgcca gtttgaaatg tttgttgaaa aataggtgtg aaacgtcaca
     7201 taaatatcaa ttttattgat taagtacaaa cttgtgaatt gaatcgcaaa cgtaaactta
     7261 gtaaaattaa gatattaaat gaaacaggag gcttttatag atgactcata ttcaattaga
     7321 ttttagtaaa acgttagaat ttttcggtga acacgaatta aaacaacaac aagaaattgt
     7381 taaatcaatt cacaaaacaa ttcatgaagg tactggtgca ggtagtgact tcttaggctg
     7441 ggttgattta ccagttgatt acgacaaaga agaattttca agaattgttg aagcatcaaa
     7501 acgcattaaa gaaaattctg atgttttagt agtcatcggt attggtggtt cttacttagg
     7561 tgcacgtgca gcaatcgaaa tgttaacgtc atcatttaga aacagcaatg aataccctga
     7621 aattgtattt gttggtaatc acttatcatc aacatatacg aaagagttag ttgattattt
     7681 agcagacaaa gatttctctg taaacgttat ttctaaatct ggtacaacta cagaaccagc
     7741 agttgcattt agattgttca aacaattagt tgaagaaaga tacggtaaag aagaagcaca
     7801 aaaacgtata tttgcaacaa cggataaaga aaaaggtgct ttaaaacagt tggctacaaa
     7861 cgaaggttat gaaacgttta tcgtacctga tgatgtaggt ggaagatatt ctgttttaac
     7921 agcagtagga ttattaccaa ttgcaacagc tggaattaac atcgaagcta tgatgattgg
     7981 tgctgcaaaa gcacgtgaag aattatcttc agataaatta gaagacaaca ttgcatacca
     8041 atatgcgaca attcgaaaca ttttatatgc aaaaggttat acaacagaaa tgttgattaa
     8101 ctatgaacca tctatgcaat actttaatga atggtggaaa caattatttg gtgaatcaga
     8161 aggtaaagac ttcaaaggta tctatccttc aagtgccaac tacacaactg atttacattc
     8221 tttaggtcaa tatgtacaag aaggccgtcg tttcttattc gaaacagtgg taaaagtaaa
     8281 tcatcctaaa tatgatatta ctattgaaaa agatagtgat gatctagacg gattaaatta
     8341 tttggctggt aaaacaatcg acgaagttaa cacaaaagca ttcgaaggta cattattagc
     8401 gcatactgat ggtggtgttc ctaacatggt agtgaacatt ccacaattag atgaagaaac
     8461 tttcggttat gtcgtatact tcttcgaact tgcttgtgca atgagtggat accaattagg
     8521 tgtaaatcca tttaaccaac ctggtgtaga agcatataaa caaaacatgt tcgcattatt
     8581 aggtaaacct ggttttgaag acttgaaaaa agaattagaa gagcgtttat aaaatacatt
     8641 acttcaatga ttagtgaagt ttgaaaagat agaactagac gttaactatt taaaacatat
     8701 tttcgaggtt gtcattacaa atgtaaaaat gtaatgacaa cctcgttttt atttatatgc
     8761 aagaactagg t
//
LOCUS       DAHHSC010000100         8662 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.101, whole genome shotgun sequence.
ACCESSION   DAHHSC010000100 DAHHSC010000000
VERSION     DAHHSC010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 8662)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8662)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8662
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.101"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            complement(259..408)
                     /gene="rpmG"
                     /locus_tag="NBI57_001696"
     CDS             complement(259..408)
                     /gene="rpmG"
                     /locus_tag="NBI57_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001265708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HCD1822635.1"
                     /translation="MRVNVTLACTECGDRNYITTKNKRNNPERIEMKKYCPRLNKYTL
                     HRETK"
     gene            complement(499..2016)
                     /locus_tag="NBI57_001697"
     CDS             complement(499..2016)
                     /locus_tag="NBI57_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000082539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase"
                     /protein_id="HCD1822636.1"
                     /translation="MSQQDKKLTGVFGHPVSDRENSMTAGPRGPLLMQDIYFLEQMSQ
                     FDREVIPERRMHAKGSGAFGTFTVTKDITKYTNAKIFSEIGKQTEMFARFSTVAGERG
                     AADAERDIRGFALKFYTEEGNWDLVGNNTPVFFFRDPKLFVSLNRAVKRDPRTNMRDA
                     QNNWDFWTGLPEALHQVTILMSDRGIPKDLRHMHGFGSHTYSMYNDSGERVWVKFHFR
                     TQQGIENLTDEEAAEIIATDRDSSQRDLFEAIEKGDYPKWTMYIQVMTEEQAKNHKDN
                     PFDLTKVWYHDEYPLIEVGEFELNRNPDNYFMDVEQAAFAPTNIIPGLDFSPDKMLQG
                     RLFSYGDAQRYRLGVNHWQIPVNQPKGVGIENICPFSRDGQMRVVDNNQGGGTHYYPN
                     NHGKFDSQPEYKKPPFPTDGYGYEYNQRQDDDNYFEQPGKLFRLQSEDAKERIFTNTA
                     NAMEGVTDDVKRRHIRHCYKADPEYGKGVAKALGIDINSIDLETENDETYENFEK"
     gene            2220..3674
                     /locus_tag="NBI57_001698"
     CDS             2220..3674
                     /locus_tag="NBI57_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCD1822637.1"
                     /translation="MEDNKMNRSLNSRHISMIAIGGAIGTGLFVATGNIISQAGPGGA
                     ILAYLVIGVMLYFLMSSIGELATFYPVSGSFSSYSTRFIDSSLGFTMGWLYWALWSLV
                     TSVDVIVASNVLYFWDTFKFFHPITWSLIFITILLLLNIFSVKSFGETEFWLSLIKVL
                     TIIVFVIFGFLMIFGILGGHTYGFENYTKGQAPFVGGISGFLGVLLVAGFSVGGTEVV
                     AVTAGESDDPKKSMPKAIKQVFWRILLFYVLSIAVIGAIIPYTDPSLLRASSSISQSP
                     FTIVFDRVGIAFAASVINAVILTSLLSAANSGVYTTGRMLYSLSSDKKAPQFLSKLNK
                     TTKLPLRALLTTYAVVVIVIIYANFNSNAVFNLLEIIGSMIIVVWGSSIWSQIRLRQA
                     IKKQGQDPNKVLPYKAPFYPLGPIIVITTLLFLLFGGSVEYILKDQWLNAFKNFLPLI
                     ILALIYFIHKIIHKTKFVKLETINLKPHDYDNQK"
     gene            3813..3917
                     /locus_tag="NBI57_001699"
     CDS             3813..3917
                     /locus_tag="NBI57_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1822638.1"
                     /translation="MLPFFILEILHIVYNIDNNNFSGVKFNDWTNKFI"
     gene            3892..4206
                     /locus_tag="NBI57_001700"
     CDS             3892..4206
                     /locus_tag="NBI57_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1822639.1"
                     /translation="MIGQTNLFDDVLKQDERFVIVVQALEEKNGQLLKRTLREYPGLN
                     HKQMNDLFTHLKELFSEESFAENQSAFSITVYTNLDYTADQIYAHVKRFRGKHDWTQT
                     AK"
     gene            complement(4499..5302)
                     /locus_tag="NBI57_001701"
     CDS             complement(4499..5302)
                     /locus_tag="NBI57_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HCD1822640.1"
                     /translation="MTNYKVVVLDMDDTLLNSDNVISEETANYLTAIQDEGYYVVLAS
                     GRPTEGMIPTARDLKLPEHHSYIISYNGSKTINMTNEEVEVSKSIGKQDFDEIVDYCR
                     DRGFFVLTYHDGQIIYDSEHEYMNIEAELTGLPMKRVDDIKAYIQGDVPKVMGVDYVA
                     NITEARIDLNGVFNDNVDATTSKPFFLEFMAKDVSKGNAIKALCHKLGYSVDQVIAFG
                     DSMNDKSMFEVAGLAIAMGNASDELKQYADEVTLDHNENGIPHALKKLL"
     gene            complement(5360..6274)
                     /locus_tag="NBI57_001702"
     CDS             complement(5360..6274)
                     /locus_tag="NBI57_001702"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HCD1822641.1"
                     /translation="MSNVLELTIPASTANLGVGFDSIGMALDKFLHLSVKETSGTKWE
                     YIFHDDASKQLPTDETNFIYHVAQQVASKYSVDLPILCIEMRSDIPLARGLGSSASAL
                     VGAIYIANYFGDIQLSKHEVLQLATEIEGHPDNVAPTIYGGLIAGFYNDVSKETSVAH
                     IDIPDVDVIVTIPTYELKTEASRRALPQKLTHSEAVKSSAISNTMICALAQHNYELAG
                     KLMQQDGFHEPYRQHLIAEFDEVKTIASQHNAYATVISGAGPTILIFSRKENSGELVR
                     SLNSQVVSCHSELVDINISGVKERIVYQ"
     gene            complement(6276..7337)
                     /locus_tag="NBI57_001703"
     CDS             complement(6276..7337)
                     /locus_tag="NBI57_001703"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HCD1822642.1"
                     /translation="MRRWQGLVEEFKAHLPVNENTPKLTLNEGNTPLIHCENMSKILG
                     IDLYVKYEGANPTGSFKDRGMVMAVTKAKEQGKKIVICASTGNTSASAAAYAARAGLK
                     AIVVIPEGKIALGKLSQAVMYGAEIVSIEGNFDEALEIVKEIAKSGEIELVNSVNPFR
                     IEGQKTGSFEIVQQLDGEAPDILAIPVGNAGNITAYWKGFKEYHEAKGSQLPKMFGFQ
                     AEGASPIVQNKVIKNPETIATAIRIGNPASWDKATNALKESNGLIDSVTDDEILEAYQ
                     LMTTKEGVFSEPASNASIAGLIKLHRQGKLPQGKKVVAILTGNGLKDPDTAISLLDNP
                     IKPLQNDKDSIIDYIKGAL"
     gene            complement(7343..8623)
                     /locus_tag="NBI57_001704"
     CDS             complement(7343..8623)
                     /locus_tag="NBI57_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HCD1822643.1"
                     /translation="MKKLNIALLGLGTVGSGVVKIIEENRQQIQDTLNKDIVIKHILV
                     RDKSKKRPLNISQYHLTEDVNEILNDDSLDIIVEVMGGIEPTVDWLRTVLKNKKHVIT
                     ANKDLLAVHLKLLEDLAEENGVALKFEASVAGGIPIVNAINNGLNANNISKFMGILNG
                     TSNFILSKMTKEQTTFEEALDEAKRLGFAEADPTDDVEGVDAARKVVITSYLSFNQVI
                     KLNDVKRRGISGVTLTDINVADQLGYKIKLIGKGIYENGKVNASVEPTLIDKKHQLAA
                     VEDEYNAIYVIGDAVGDTMFYGKGAGSLATGSAVVSDLLNVALFFESDLHTLPPHFEL
                     KTDKTREMMDSDAEINIKEKSNFFVVVNHVKGSIENFENELKAILPFHRSLRVANYDN
                     QSYAAVIVGLESSPEELITKHGYEVDKVYPVEGV"
BASE COUNT         2676 a         1631 c         1233 g         3122 t
ORIGIN      
        1 gaaattttaa attctactat attaaatact ctttcaagca ttcgctaaca aagtgacttt
       61 ataaaatcag taatctattt ccttctataa atattctcaa attttaataa agtaacaact
      121 aatacaatca tatattgttc taccgatgaa actcaatcca ttaatcactc ttcacaaaaa
      181 tttcataaaa aagcgagctc cccaatataa taggtaagcc cgctttattt tcaaatcgtc
      241 gtatttgaat gataagaatt acttagtttc acgatgtaac gtatatttgt ttaatcttgg
      301 gcaatatttt ttcatttcaa tacgctcagg attattacgt ttatttttag tagtgatata
      361 gttacgatcg ccacattctg tgcatgctaa tgttacgttt acgcgcacgt taaacaccct
      421 ctcttgttag gaatctttac gatttattat gatatcataa actgctcaac tacgcaatat
      481 agaaactaca tatcaaattt atttttcaaa gttttcgtat gtttcatcat tttcagtttc
      541 aagatcaata gaatttatat caatacctaa tgcttttgca acacctttac catattctgg
      601 gtcagctttg taacaatgac gaatatgacg tcgtttaaca tcatccgtta cgccttccat
      661 tgcatttgct gtatttgtaa aaattctttc tttagcgtcc tctgattgta atctaaacaa
      721 tttacctggt tgttcaaaat aattatcatc atcttgacgt tgattatatt catagccgta
      781 tccatcagtt gggaatggtg gctttttata ttcaggttga gaatcaaatt taccatggtt
      841 atttggataa taatgtgttc ctccaccttg gttattgtca actacgcgca tttgaccatc
      901 tctactaaaa ggacaaatat tttcaatacc aacaccttta ggttggttta caggaatctg
      961 ccaatgatta actcctaatc gatatctttg cgcatcgcca tatgagaata aacgcccttg
     1021 caacattttg tctggagaaa aatctaatcc tggaataata ttagttggtg caaacgcagc
     1081 ttgttcaaca tccataaagt aattatctgg atttctattt aattcaaact ctccaacttc
     1141 aattagagga tactcatcgt gataccatac ttttgttaaa tcaaatggat tatctttatg
     1201 gtttttagct tgttcctcag tcattacttg aatatacatt gtccattttg gataatcacc
     1261 tttttcaatg gcttcgaata aatcgcgttg agatgaatca cgatctgtag ctataatttc
     1321 agcagcttct tcatcagtta agttttcaat accttgttgc gttctaaaat ggaatttaac
     1381 ccaaacacgt tcaccagaat cattatacat agagtatgtg tgagaaccga acccatgcat
     1441 atgacgtaaa tctttaggaa tccctctatc tgacattaag atcgttactt ggtgcaatgc
     1501 ttctggaaga cccgtccaga aatcccagtt attttgtgca tctctcatat ttgttctagg
     1561 atctcgtttc accgcacgat ttaaactaac aaataacttt ggatctctaa agaagaatac
     1621 tggtgtgtta ttccctacta aatcccagtt cccttcttca gtgtagaact ttaacgcaaa
     1681 tcctcgaatg tcacgctccg catcagcagc accacgttct cctgctacag tagagaaacg
     1741 ggcaaacatt tcggtttgct tacctatttc agagaatatt ttagcattcg tatattttgt
     1801 tatatcttta gttacagtaa atgtcccaaa tgcaccagaa cctttggcat gcattcgacg
     1861 ttctggtatt acttctctat caaattgaga catttgctct aaaaagtaaa tatcttgcat
     1921 taaaagaggt cccctaggcc ctgctgtcat actattttct cggtctgata ctggatgccc
     1981 aaaaacacca gttaactttt tgtcttgttg tgacatagtc atccctccac aatttataat
     2041 aattataatt taatacatat tataatttaa ccccatcaaa attacaatta aacatgctca
     2101 agatgattaa aaaatcagaa aattttaatt ttttcgcttt acagtactaa atagttattg
     2161 aatagtacac ttaaaaagct tacaataatg ggtatattta aatagatggg agactaaata
     2221 tggaagataa taaaatgaac cgtagtctta actcaagaca catttccatg attgctatag
     2281 gtggtgcaat tgggactggt ctatttgtag ctactggtaa tatcatttct caagctggtc
     2341 ctggaggcgc tatactcgct tatcttgtta ttggtgtcat gctatatttc ttaatgtcat
     2401 caattggaga gttggcaaca ttttatccag tatcaggttc attcagctct tattcaacac
     2461 gctttattga ctcatctctt ggctttacca tgggatggtt gtattgggca ttgtggtcat
     2521 tagttacaag tgttgatgtc atagtagcgt caaatgtgct ttatttctgg gacacattta
     2581 aatttttcca ccccattact tggagcttaa tctttattac aattttacta ttattaaaca
     2641 ttttttctgt aaaatcattt ggagaaactg agttttggtt atcattgatt aaagtgttaa
     2701 caattatcgt attcgttatt tttggctttt taatgatttt cggtatctta ggtggtcata
     2761 catatggatt tgaaaactat acaaaaggcc aagcaccgtt tgttggtggt atctctggtt
     2821 tcttaggcgt attattagtc gccggatttt cggttggtgg tacagaagta gtagcagtaa
     2881 ctgctggtga atcagatgac cctaaaaagt ctatgcctaa ggcaattaaa caagtatttt
     2941 ggcgtattct tttattctat gtcttatcaa ttgcagtaat tggtgcaatt attccgtaca
     3001 cagatccatc attattaaga gcaagtagtt caataagtca aagcccattt acaattgtat
     3061 tcgatagagt aggcatagcc tttgcagcat cagtaatcaa cgcggttatt ttaacttcat
     3121 tattatccgc tgcaaattca ggtgtttata caacaggcag aatgttgtat tccttaagtt
     3181 cagacaaaaa agcaccccaa tttttaagta aattaaacaa gacaactaag ttacctttaa
     3241 gagcattatt aactacttat gcagtcgttg ttattgttat tatttatgca aactttaatt
     3301 caaatgccgt ttttaattta cttgaaatta ttggttcaat gattatagtt gtttggggat
     3361 caagcatttg gtcacaaata cgattgcgac aagctattaa aaaacaaggt caagacccta
     3421 ataaggtcct accatataaa gcaccttttt atccattagg accaatcatt gtcatcacta
     3481 cactattatt cttgctattt ggtggctcag ttgaatatat tttaaaagat caatggttaa
     3541 atgcttttaa aaacttttta cctttaatca ttctagcgtt gatttacttt attcataaaa
     3601 tcattcacaa aacaaaattt gtaaagctag aaacaattaa tttaaaacca cacgattatg
     3661 acaatcaaaa ataaataaat tcttacacgg cagatgcttt agattatcta tagcatttgc
     3721 cgctttttaa tggtgtaaag cagatatact ttaatatctt tttctagtat aaagtgtatt
     3781 aactagtatt ctgtttatat tttattatta ccatgttacc attctttatt ttagaaattt
     3841 tgcatatcgt gtataatata gataacaata acttttcagg ggtgaaattc aatgattgga
     3901 caaacaaatt tatttgatga tgtactaaag caagatgaaa gatttgttat tgtagttcaa
     3961 gcattagaag aaaagaacgg acaattatta aagagaactt taagggaata tcccggttta
     4021 aaccataaac aaatgaatga tttatttacg cacttaaagg aattattttc cgaagaatca
     4081 tttgctgaaa accaatcagc gttcagtatt acagtttata caaatttaga ttatactgct
     4141 gaccaaatat atgctcatgt aaaacgtttc agaggtaagc atgactggac acaaacagct
     4201 aaataaaatc aagatgcttg cagtgatttt aacaattaat gttagattgt cactgcattt
     4261 tttaatgcat tcgttattat tcagaatgat caaatttatt tacctacctt ccgtgactag
     4321 ttaatacttg atttattcaa aaaatataca tttactctat tcgtttgata tcattactaa
     4381 tctaaaatat gtatttcaaa tcctccatag tataactgaa gtcattttca cataaataaa
     4441 aagccttaac cacaagatat cttattcaaa tatcatgtgt taaggcttat tttaaaattt
     4501 ataacaattt tttgagcgca tgtggaatac cattttcatt atggtccaac gtaacttcat
     4561 ctgcatattg cttaagttca tctgatgcat tccccatagc aatagctaga cctgcgactt
     4621 caaacattga tttatcattc atactatcac caaaagcaat gacttgatcc accgaatatc
     4681 ccaatttgtg acataacgct ttaattgcat taccttttga aacatctttg gccataaatt
     4741 ctaagaagaa tggcttactt gtcgtagcat ctacattatc attgaacaca ccattcaaat
     4801 caattctagc ttctgtaata ttcgctacat aatctacacc catgaccttg ggtacatcgc
     4861 cttgaatata cgctttgata tcatcaacac gtttcatcgg taaacctgtt aattctgctt
     4921 caatattcat atactcatgt tcgctgtcgt aaataatttg accatcatga tatgtaagaa
     4981 cgaaaaagcc tctgtctcga caataatcta caatttcatc gaaatcttgc ttaccaatcg
     5041 atttacttac ttctacttct tcattagtca tgttaatcgt tttactaccg ttataactaa
     5101 taatatagct atgatgttca ggtaatttta aatctctagc agttggaatc ataccttcag
     5161 taggtctacc agatgctaaa acaacataat aaccttcatc ttgaattgct gttaaataat
     5221 ttgcagtttc ttctgatatc acattatctg aatttagcaa tgtgtcatcc atatctaaaa
     5281 cgacaacttt ataatttgtc atatcgcatt aaatctcctc tctattctgc atttaatact
     5341 attttacaat ataaagcgtc tattggtata caattcgttc tttaacacca ctgatattaa
     5401 tatcgaccaa ttcagaatgg catgatacta cctgactatt taaagagcga accaattccc
     5461 cactattttc tttacgacta aatattaaaa tagttggtcc agcaccacta attacagttg
     5521 cataggcatt atgttgacta gcaattgttt tcacttcatc aaattcagca attaaatgct
     5581 gacgatacgg ttcatgaaag ccatcttgtt gcatgagttt acctgctaat tcataattgt
     5641 gctgtgctaa tgcacaaatc attgtattac taattgcact actttttacc gcttcactat
     5701 gtgttaattt ttgtggtaaa gcacgtcttg atgcttctgt ttttagttca taagttggta
     5761 tcgttacaat cacatccacg tctggtatgt cgatatgtgc aactgacgtt tctttcgaga
     5821 catcattata aaatccagcg attaaaccac cataaatggt cggcgcaaca ttatcaggat
     5881 gtccttcgat ttcagtcgct aattgtaata cctcatgttt agacagttgg atatcaccaa
     5941 aataatttgc gatatatata gctcctacta aagcagaagc tgacgaacct aaccctcttg
     6001 ccaatggaat atcacttctc atttcgatac ataaaatagg caagtcaaca ctatatttag
     6061 aagcaacttg ttgtgctaca tgataaataa agtttgtttc gtcagtaggc aattgcttag
     6121 atgcatcatc atggaaaata tattcccatt ttgtccctga tgtttccttt acagacagat
     6181 gcaaaaattt atctaaagcc atacctatag aatcaaagcc aactccaagg ttggctgttg
     6241 atgcaggaat tgttaactcc aaaacattcg acatgttata aagctccttt aatataatcg
     6301 ataatgctat ctttatcatt ttgcaatggc tttatcggat tatctagtag tgaaatagca
     6361 gtatcaggat cttttaatcc attaccagtt aaaatagcaa ctactttttt accttgaggt
     6421 aatttacctt gtctatgcaa tttaattaaa cctgcaatag aagcattact tgctggttca
     6481 ctaaagacac cttctttagt tgtcattaac tgatatgctt ctagaatttc atcatcagta
     6541 acactatcta ttaatccatt tgattcttta agagcattag tcgccttatc ccaactagca
     6601 ggattaccaa ttcgaatagc agttgcaatc gtttcaggat ttttaatgac tttattttga
     6661 acaattggtg atgcgccttc agcttggaag ccaaacattt tcggcaattg tgatccttta
     6721 gcttcatgat attctttaaa gcctttccaa tatgcagtaa tattacctgc attacctaca
     6781 ggaatcgcta aaatatcagg tgcttcaccg tctaattgtt gtacaatttc aaatgagcct
     6841 gtcttttgcc cttcgattct aaatggattg acagagttta caagctctat ttcgccactt
     6901 tttgcaattt cttttacaat ttctaaagct tcatcaaagt ttccttcaat agaaacgatt
     6961 tctgcaccat acattactgc ttgcgacaat ttacctaatg caattttacc ttctggtatt
     7021 acgacgatag cttttaaacc tgctctcgct gcatatgctg ctgcagacgc tgatgtattt
     7081 ccagtcgaag cgcatattac aattttctta ccttgctctt ttgcttttgt cacagccatt
     7141 accataccgc gatctttaaa tgaacctgtc ggattggcac cttcatactt cacatataaa
     7201 tctataccta gtattttaga catattttca caatgaatga gtggtgtatt tccctcgttc
     7261 aatgttaatt ttggtgtatt ttcatttact ggtaaatgtg ctttaaactc ttctactaat
     7321 ccttgccatc ttctcattat aattaaactc cttctactgg gtatactttg tcaacttcgt
     7381 atccatgctt agtgattaat tcttccggtg atgattccaa tccaactata acagcggcat
     7441 atgattgatt atcgtaattt gcaactctta atgatcggtg aaatggtaat attgccttta
     7501 actcattttc aaaattttca attgaacctt tgacatgatt cactactaca aagaaattgg
     7561 atttttcttt aatattaatt tctgcatctg aatccatcat ttcccttgtt ttatctgtct
     7621 ttaattcaaa atgtggtggc aatgtgtgta aatctgattc aaagaataat gctacattca
     7681 ataaatcact gacaacggca ctacctgttg ctaaactgcc tgctcctttt ccataaaaca
     7741 tcgtgtcacc aacggcatca ccaataacat aaatcgcgtt atattcatcc tctacagctg
     7801 ctaattgatg ctttttatca attaacgttg gttctaccga tgcattaact ttgccatttt
     7861 catatattcc cttaccaatc aatttaattt tataccccag ttgatcggct acattaatat
     7921 cagttaaagt tacgccacta attcctcttc gtttaacgtc gtttaattta atgacttggt
     7981 taaatgataa atatgatgta atgacaactt tacgcgctgc atctacccct tctacatcat
     8041 cagttggatc cgcttcagca aaaccaagtc ttttcgcttc atcaagtgct tcctcaaatg
     8101 tcgtttgctc tttagtcatt ttagataaaa taaaattaga ggtaccattt aaaattccca
     8161 taaattttga aatattattc gcattcaaac cattatttat ggcatttacg atcggaatac
     8221 cacctgctac actcgcttca aactttaaag ctacaccatt ttcttctgct aaatcttcta
     8281 aaagtttaag atgtactgct aataaatctt tatttgcggt aataacatgt tttttatttt
     8341 taagtactgt tcttaaccaa tctacagttg gttcaattcc tcccatgact tcaacgataa
     8401 tatctaatga atcatcattt aaaatttcat taacatcttc agttaaatga tattggctaa
     8461 tatttagcgg tctcttttta gatttatctc gaacaagaat atgctttatg acaatatctt
     8521 tatttaatgt atcttgaatt tgctgtcggt tctcttcgat gattttaaca acaccagatc
     8581 cgacagtacc taatcctaat aatgctatat ttaatttttt cattttatgt acctccgttt
     8641 gtgcttttga gaaaaacaaa tg
//
LOCUS       DAHHSC010000101         8537 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.102, whole genome shotgun sequence.
ACCESSION   DAHHSC010000101 DAHHSC010000000
VERSION     DAHHSC010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 8537)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8537)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8537
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.102"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            181..1266
                     /locus_tag="NBI57_001705"
     CDS             181..1266
                     /locus_tag="NBI57_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HCD1822644.1"
                     /translation="MLNKVWFRTGIALIMLFILIKLFMEVHEVFTPIATIIGSVFLPF
                     LISGFLFYICLPFQNLLEKVGFPRWASITTIMLALFAIIGLIVAFVAPIIISNINNLI
                     SQTPALQKEAEQIIKFALAQMDKLPEDVTSRITNMVKSMGDGVTNILSNSLQYITSLI
                     STIFLLIMVPFFLIYMLKDHERFIPAVAKFFKGERKVFFVDLLTDLNFTLKSYIQGQV
                     TVSVILGIFLYIGYSIIDLPYIPLLVLFAGVANLIPFLGSWLSFAPAAILGIIDSPTT
                     FIWVCIITLIAHQLEGNIITPNVMGKSLSIHPLTIIVVILAAGDLGGFTLVLIAVPLY
                     AVLKTVVSNIFKYRQRIIDKANSNVKD"
     gene            complement(1463..2233)
                     /gene="fabI"
                     /locus_tag="NBI57_001706"
     CDS             complement(1463..2233)
                     /gene="fabI"
                     /locus_tag="NBI57_001706"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HCD1822645.1"
                     /translation="MLNLENKTYVIMGIANKRSIAFGVAKVLDQLGAKLVFTYRKERS
                     RKELEKLLEQLNQPEAHLYQIDVQSDEEVINGFEQIGKDVGNIDGVYHSIAFANMEDL
                     RGRFSETSREGFLLAQDISSYSLTIVAHEAKKLMPEGGSIVATTYLGGEFAVQNYNVM
                     GVAKASLEANVKYLALDLGPDNIRVNAISAGPIRTLSAKGVGGFNTILKEIEERAPLK
                     RNVDQVEVGKTAAYLLSDLSSGVTGENIHVDSGFHAIK"
     gene            complement(2511..4355)
                     /locus_tag="NBI57_001707"
     CDS             complement(2511..4355)
                     /locus_tag="NBI57_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation:proton antiporter family
                     protein"
                     /protein_id="HCD1822646.1"
                     /translation="MEFLSLVIVVLAAFLTPIIVNRLNINFLPVVVAEILMGIVIGNS
                     FLNIVERDSILNILSTLGFIFLMFLSGLEIDFKAFKKDKRARQGQNDDESSIPGHLNL
                     ALTVFAFIMIISILLAYVFKWLGLVDDVLLMVIIISTISLGVVVPTLKEMNIMRTTIG
                     QFILLVAVLADLVTMILLTVYGAINGQGGSTIWLIGILVVFTAISYILGVQFKRMSFL
                     QKLMDGTTQIGIRAVFALIILLVALAEGVGAENILGAFLAGVVVSLLNPDEEMVEKLD
                     SFGYGFFIPIFFIMVGVDLNIPSLIKEPKLLIIIPILIVAFIISKLIPVMFIRRWFDM
                     KTTIASAFLLTSTLSLVIAAAKISERLNAISAETSGILILSAVITCVFVPIIFKKLFP
                     VPDEFNRKIEVSLIGKNQLTIPIAQNLTSQLYDVTLYYRKDLSDRRQLSDDITMIEIA
                     DYEQDVLERLGLFDRDIVVCATNDDDINRKVAKLAKAHQVERVICRLESTTDDTELVD
                     SGIEIFSSYLSNKILLKGLIETPNMLNLLSNVETSLYEIQMLNYKYENIQLRNFPFGG
                     DIIFVRIIRNNESIVPHGDTQLRYGDRLIVTGAKEYVDELKQELEFYF"
     gene            complement(4365..5750)
                     /gene="mgtE"
                     /locus_tag="NBI57_001708"
     CDS             complement(4365..5750)
                     /gene="mgtE"
                     /locus_tag="NBI57_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HCD1822647.1"
                     /translation="MSMNTDEKERVQEELYDETLLDQYLENDDIDQFRDEFLALHTYE
                     QSEYFEDTTDENRQKIFQYLSPEEVANFFDQLDIDDDEYELLFDKMNATYASHILEEM
                     SYDNAVDILNELTKPKVASLLTLMNKDDANEIKALLHYDEDTAGGIMTTEYLSLKAHT
                     PVKEALLLVKAQAPDAETIYVIFVVDDDGKLVGVLSLRDLIVAENDAYIEDIMNERVI
                     SVNVADDQEDVAQVMRDYDFMAVPVIDYQEHLLGIITIDDILDVMDEEASEDYSRLAG
                     VSDIDSTNDSIIKTALKRLPWLIILTFLGMITATILGRFEKTLENVALLAAFIPIISG
                     MSGNSGTQSLAVSVRNITTGEINEQSKFRIALREAGSGVLSGVVCSTILFTIIVAIYH
                     QPLLALIVAGSLTCAMTVGTFVGSMIPLLMNKLNIDPAVASGPFITTINDIISMLIYF
                     GLATSFMAYLI"
     gene            complement(5771..6625)
                     /locus_tag="NBI57_001709"
     CDS             complement(5771..6625)
                     /locus_tag="NBI57_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HCD1822648.1"
                     /translation="MKFKYHISQQETVKAFLARHDFSKKTVSAIKNNGALIVNDEPVT
                     VRKQLMPNDILEIHLPREIPSVNLIPYARKLEVLYEDAFIIIVTKPNNQNCTPSREHP
                     HESLIEQVLYHCQEHGENINPHIVTRLDRNTTGIVIFAKYGHIHHLFSKVNLKKIYTC
                     LVYGKTHTSGIIEANIRRSKDSIITREVASDGKYAKTSYEVINQNDKYSLCKVHLHTG
                     RTHQIRVHFQHIGHPIVGDSLYDGFHDKIHGQVLQCTQIYFVHPINKNNIYITIDYKQ
                     LLKLFNQL"
     gene            complement(6622..7431)
                     /locus_tag="NBI57_001710"
     CDS             complement(6622..7431)
                     /locus_tag="NBI57_001710"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HCD1822649.1"
                     /translation="MRYTILTKGDSKSNALKHKMMNYMKDFRMIEDSENPEIVISVGG
                     DGTLLQAFHQYSHMLSKVAFVGVHTGHLGFYADWLPHEVEKLIIEINNSEFQVIEYPL
                     LEIIMRYNDNGYETRYLALNEATMKTENGSTLVVDVNLRGKHFERFRGDGLCVSTPSG
                     STAYNKALGGALIHPSLEAMQITEIASINNRVFRTVGSPLVLPKHHTCLISPVNHDTI
                     RMTIDHVSIKHKNVNSIQYRVANEKVRFARFRPFPFWKRVHDSFISSDEER"
     gene            complement(7448..8083)
                     /locus_tag="NBI57_001711"
     CDS             complement(7448..8083)
                     /locus_tag="NBI57_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HCD1822650.1"
                     /translation="MNQWDQFLTPYKQAVDELKVKLKGMRKQYEVGEQASPIEFVTGR
                     VKPIASIIDKANKRQIPFDRLREEMYDIAGLRMMCQFVEDIDVVVNILRQRKDFKVIE
                     ERDYIRNTKESGYRSYHVIIEYPIETLQGQKFILAEIQIRTLAMNFWATIEHTLRYKY
                     DGAYPDEIQHRLERAAEAAYLLDEEMSEIKDEIQEAQKYYTQKRSKKHEND"
     gene            complement(8100..8447)
                     /locus_tag="NBI57_001712"
     CDS             complement(8100..8447)
                     /locus_tag="NBI57_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1822651.1"
                     /translation="MRLYINEIKIKDDILYCYTEDSIKGLSEVGQMLVDSDNYAFAYT
                     LDDGKAYAYLIFVQETWTMLHENTTKKIIINDELELTEFHQELTYILDNIKGNNNYGK
                     EFVATVEETFDIE"
BASE COUNT         2791 a         1564 c         1181 g         3001 t
ORIGIN      
        1 atccaattgc agacttacga gtcattgaaa agtctcacaa actattgaaa gtaaatatct
       61 taaaatagaa agtgaatggt taatttaagt atatttaaaa aatattaacc tttttaagca
      121 ctgctattta ggatatacta aataataact aagtttagaa aaataggagg aacatcgttt
      181 atgttaaaca aggtttggtt ccgaactggc atcgctctga ttatgctgtt cattctcatc
      241 aaactattta tggaagtgca cgaagtattt actccaatag ctacaatcat cggttctgta
      301 tttcttccat ttttaattag tggtttttta ttttatatct gtctaccttt tcaaaactta
      361 cttgaaaaag tcggttttcc aagatgggct agtataacaa caatcatgtt agctttgttt
      421 gctattatcg gcttaattgt tgcgtttgtt gcaccaatca tcatttcaaa tatcaacaat
      481 ttaattagtc agacacctgc cctacaaaaa gaagcagagc aaattattaa attcgcacta
      541 gctcaaatgg ataaattacc tgaagatgta acaagtagaa ttacaaatat ggttaaatca
      601 atgggtgatg gcgttactaa tattttatct aattcattac agtacattac atcattaatt
      661 tcaaccatct tcttattgat tatggttcca ttcttcttaa tttatatgtt aaaagaccat
      721 gaaagattta tcccagctgt tgctaaattt ttcaaaggtg aacgtaaagt atttttcgta
      781 gatttattaa ctgatttaaa ctttacatta aaatcttaca ttcaaggtca agtaactgtc
      841 agtgttattt taggtatatt cttatatatc ggttattcaa ttattgactt accatatatt
      901 cctttattag ttctatttgc tggtgtcgca aacttaattc ctttcttagg ttcatggtta
      961 tcatttgcac ctgcagcaat actcggtatt atagatagtc ctactacatt tatatgggtt
     1021 tgtatcatta cattaattgc acaccaactt gaaggtaata tcattacgcc aaatgtaatg
     1081 ggtaaatctt taagtatcca tcctttaaca attatcgttg ttattttagc agcaggagat
     1141 ttaggtggct ttacattagt tctgattgca gtgccattat atgctgtact taaaacggtt
     1201 gttagtaata ttttcaaata ccgccaacgc attattgata aagcaaacag taatgttaag
     1261 gactaattct gtggatgtct tttaagaata taagatacta tctcctcaaa agttgaaact
     1321 acagcttttg aggagatttt tttgtgcata aaaaaaatca gtcagatgaa atatcaaata
     1381 attttccatc agtccgatta ttataaaagc aaaaaagctt tgctcacata tatgataacg
     1441 tgaacaaagc tgttgaatga tattatttaa ttgcgtggaa tccgctatct acatgaatat
     1501 tttcacctgt aacgccactt gataagtcac ttaataagta agccgctgtt ttacctactt
     1561 ctacttgatc aacgttacgt tttaaaggtg cacgctcttc gatttcttta agaattgtat
     1621 tgaaaccacc cacacctttt gcacttaatg tacggattgg accagctgaa attgcattaa
     1681 cgcgaatatt atcaggacct aagtctaatg ctaaatattt aacatttgct tctaagctcg
     1741 ctttagcaac acccatcaca ttataatttt gaactgcgaa ttcgccacct aaatatgttg
     1801 ttgcaacaat gctaccacct tctggcatta attttttagc ttcatgagcc acaattgtta
     1861 atgagtaaga actaatgtct tgagctaaca agaagccttc acgtgaagtt tcagaaaagc
     1921 gtccgcgtaa gtcttccata ttagcaaatg cgattgaatg atatacacca tcaatattgc
     1981 caacatcttt accaatttgc tcaaaaccat taataacctc ttcatcgctt tgaacatcaa
     2041 tttgatataa gtgcgcttct ggttgattta attgttctaa taatttttca agctctttac
     2101 ggctacgttc tttacggtaa gtaaatacta atttagcacc taattgatct aaaactttag
     2161 cgacaccaaa agcaatacta cgcttattag cgattcccat gatgacatat gttttgtttt
     2221 caagatttaa catgtaagat aactccttat aataattaat ttaacggata gaatatctaa
     2281 tcccagtttt aactaacttt ttattcgtca atacatttta acactattat gactttttaa
     2341 taaagttatt taaacgtatt acaacgataa atgtttgatt tagtcctttc tgtatcaaac
     2401 ttgatttacg taaaaagcat ttatttatca aaatcatata catactagca ttttaaaatc
     2461 attaatttta cgtcgtacta aaatacgtct gcattttaat atcattattg ttaaaaataa
     2521 aattctaact cttgcttcaa ttcatcaacg tattctttag caccggtaac aattaagcga
     2581 tctccatatc gcaattgtgt atctccatgc ggaacaatcg actcattatt acggataata
     2641 cgcacgaaga tgatgtctcc tccgaatggg aaattacgta attgaatatt ttcatattta
     2701 taatttaaca tttgaatttc atatagtgac gtttcaacat tacttaataa attcaacatg
     2761 ttaggtgttt caattaaacc ttttaataag attttattac ttaagtagct actgaaaatt
     2821 tcaatacctg aatcaactaa ctctgtatcg tccgttgtgc tttcaagtct gcaaatgaca
     2881 cgctcaactt gatgtgcttt ggctaattta gcaacttttc ggttaatatc atcgtcattc
     2941 gtagcacaaa caactatgtc tcggtcaaac agacctagtc gttctaaaac atcttgttca
     3001 taatcagcaa tttctatcat cgtgatatca tctgacaatt gacgacgatc actcaagtct
     3061 ttgcgataat ataatgtcac gtcatataac tgagatgtta aattttgcgc tataggaatc
     3121 gttaattgat ttttaccaat taaactaact tcaattttac ggttaaactc atctggaact
     3181 ggaaacagtt ttttgaaaat aatcggaacg aatacacatg taatgactgc gcttaaaatt
     3241 aatatacctg acgtttcagc tgaaatagca tttaatcttt ctgaaatttt ggctgcagct
     3301 atcacgagcg ataatgttga tgttaataaa aatgctgatg caatcgttgt tttcatatca
     3361 aaccaacgtc gaataaacat gactggaatt aattttgaaa tgataaatgc aacgattaaa
     3421 atcggtatga taattagtaa tttcggttct ttaattaatg aaggtatgtt taaatctaca
     3481 ccaaccatta taaagaaaat aggaataaag aacccataac caaatgagtc taacttttca
     3541 accatttctt catctggatt taataatgaa acaacgacac ctgctaagaa tgcacctaat
     3601 atattttctg cgccaactcc ctctgctagg gctactaata atattattaa tgcaaatacc
     3661 gcacgaatac cgatttgcgt cgtaccatcc atcaattttt gtaaaaatga cattctttta
     3721 aattgaacac ctaaaatata tgaaattgct gtgaaaacaa ctaatatacc tattaaccat
     3781 attgtactgc cgccttgacc attgattgcg ccatagaccg ttaataaaat catagttact
     3841 aagtccgcaa gtactgctac taataggata aattgcccta tagttgttct cataatattc
     3901 atttctttta aagttggaac aactacgcct aaggaaatag ttgaaatgat aatgaccatt
     3961 aataacacat catccactaa tccaagccat ttaaatacat acgctaaaag aatcgaaata
     4021 atcataataa atgcaaatac agttaacgct agattaagat gccctggaat tgaggattca
     4081 tcatcatttt gtccttgacg tgcgcgttta tcttttttaa aagctttaaa atcaatttct
     4141 aaaccactta aaaacattaa aaagataaag cctaacgttg ataaaatatt tagaattgaa
     4201 tccctttcta ctatatttag aaatgaattt ccaatcacaa tccccatcaa aatttctgca
     4261 acaacaactg gcaagaaatt aatatttaat cgattgacaa ttattggagt taaaaacgct
     4321 gctaaaacaa caataactaa agataaaaac tccataactc ctccttaaat taagtaagcc
     4381 ataaatgatg tagctaaacc aaaataaatc aacatactaa taatatcatt aattgttgta
     4441 ataaatggtc cactagccac tgctggatcg atatttaatt tattcatcaa taatggaatc
     4501 atcgaaccta caaacgtccc caccgtcatc gcacaagtta aacttcctgc aacgattaat
     4561 gctaaaagtg gctgatgata tattgcaaca ataattgtaa ataatattgt tgaacataca
     4621 acgcccgata atacaccact tcccgcttct cttaatgcaa ttctaaattt actttgctca
     4681 ttaatttccc ctgtcgtaat gttacgaact gaaacggcta aagattgtgt acctgaattt
     4741 cctgacatac cactaataat aggaataaac gctgcgagta gcgctacatt ttctaatgtt
     4801 ttttcaaatc tccctaaaat tgtcgcagta atcattccta aaaatgttaa aataatcaac
     4861 catggtaaac gttttaatgc tgttttaatg attgaatcat tagtcgaatc gatatctgat
     4921 accccggcta aacgagagta gtcttcacta gcctcttcat ccataacgtc taaaatatca
     4981 tcaatcgtga tgataccaag caaatgttct tggtaatcta taacaggtac agccatgaaa
     5041 tcatagtctc tcataacttg agcaacatct tcttggtcgt ctgctacatt cacactaatg
     5101 acacgttcat tcataatatc ttcaatataa gcatcatttt cagctacaat taaatctctt
     5161 agtgataaaa cacctactaa tttaccatca tcatcaacga caaatataac atatattgtt
     5221 tctgcgtctg gcgcttgcgc tttgaccaat aataacgctt ctttaacagg cgtatgcgct
     5281 ttaagtgata aatactccgt cgtcataata ccgccggccg tatcctcatc atagtgaagt
     5341 aatgctttga tttcattcgc gtcatcttta ttcatcaatg ttaaaagact agcaactttt
     5401 ggtttagtca actcatttaa aatatctact gcattatcgt atgacatttc ttctaatatg
     5461 tgacttgcgt atgtcgcatt cattttatca aatagcaact catattcatc gtcatcaata
     5521 tctaattgat caaagaaatt tgcaacctct tcaggtgata aatattgaaa aatcttttgt
     5581 ctattttcat cggtagtatc ttcaaaatac tcactttgtt catatgtgtg taatgctaga
     5641 aattcatctc taaattgatc aatatcatca ttttctaagt attgatctaa taatgtctca
     5701 tcatataatt cctcttgaac acgctctttt tcatctgtgt tcattgacat acttacaccc
     5761 cctgtgtgaa ttagagttga ttgaataatt taagtaattg cttataatca attgtaatat
     5821 aaatattgtt cttattgatt ggatgaacaa aatatatttg cgtacattgc agtacttgac
     5881 catgaatttt gtcatgaaaa ccatcataca aagaatctcc cacaattgga tgcccaatat
     5941 gttgaaaatg tacacgaatt tgatgtgtac gtcccgtatg caaatgaact ttgcataaac
     6001 tgtatttatc attctgattt attacttcat aagatgtttt agcgtattta ccatccgagg
     6061 caacttctct agttataata ctatcctttg accgtctaat attagcttca ataataccag
     6121 atgtatgggt tttgccatat acaaggcaag tatatatttt tttcaagttt actttagaaa
     6181 ataaatgatg gatatgtcca tatttagcga atatcacaat accagttgta ttacgatcta
     6241 gacgcgtaac aatatgtggg ttaatatttt caccatgttc ctgacaatga tatagtactt
     6301 gttcgattaa actttcatga ggatgttctc tcgaaggcgt acaattttga ttgtttggtt
     6361 tagtaactat gatgataaaa gcatcttcat acaatacttc tagcttacga gcataaggta
     6421 ttaaattaac actcggtatt tctcgcggta aatgaatttc taatatatca tttggcatta
     6481 attgcttacg tactgtgact ggttcatcat taacaattaa agcgccatta tttttaatgg
     6541 cgctcactgt cttcttagaa aaatcatgtc gtgctaaaaa agctttaaca gtttcttgtt
     6601 gtgatatatg atacttaaat ttcatcgttc ttcatcactt gatatgaaag aatcgtgtac
     6661 acgtttccag aacgggaatg gtctaaaacg tgcaaacctc actttttcat ttgctacacg
     6721 gtattgtatt gaattaacat ttttatgttt gatactaaca tgatctatcg tcattctaat
     6781 ggtatcatga ttaactggtg atattaaaca tgtatgatgc tttggtaata caagtggtga
     6841 tcctaccgtt ctaaacacac gattatttat tgaggcaatt tctgtaattt gcattgcttc
     6901 aagtgaagga tgtatcagtg cgccacctag cgctttgtta taagccgttg aacccgaagg
     6961 tgttgataca cataatccat cgcctctaaa tcgctcaaag tgtttccctc ttaagttaac
     7021 atccacaaca agtgttgagc cattttcagt tttcatcgtt gcttcattta atgctaaata
     7081 ccttgtttca tagccgttgt cgttgtatct cataataatt tcaagcaatg gatattcaat
     7141 gacctgaaac tctgaattat taatttcgat gattaatttt tcaacttcat gaggtaacca
     7201 atccgcataa aatcctaaat gacctgtatg aactccaaca aatgccactt ttgataacat
     7261 gtggctatac tgatggaatg cttgtagtaa tgtaccatca ccaccaactg aaataacaat
     7321 ttcaggattt tcactatcct caatcatgcg aaaatctttc atatagttca tcattttatg
     7381 ctttaaggca ttagacttgg agtcaccttt agttaaaatt gtataacgca tgatttataa
     7441 cacctcgtta atcattttca tgttttttag aacgtttttg cgtgtaatat ttttgagctt
     7501 cctgaatttc atctttaatt tcagacatct cttcatcaag taaatacgct gcttctgccg
     7561 ctctttccaa acgatgttga atttcatccg gataagcacc atcatattta tatcgtaaag
     7621 tatgttcaat cgttgcccag aaattcattg ctaatgtacg aatctgaatc tcagccaata
     7681 taaatttttg gccttgtaat gtttcaattg gatattcaat aatgacatga tacgagcggt
     7741 aaccactttc tttagtgtta cgaatataat ctcgttcttc aattacttta aaatcttttc
     7801 tttgtcttaa aatattgacg acaacatcaa tatcttctac aaattggcac atcattctta
     7861 aaccagcgat atcgtacatt tcttctctta acctatcaaa tggtatttgt cgtttgtttg
     7921 ccttatctat aatactagcg atcggtttaa cacgaccagt aacaaattct attggcgacg
     7981 cttgttcacc aacttcatat tgtttgcgca tgcctttaag tttcactttc aactcatcaa
     8041 ccgcttgctt ataaggtgtt aagaactgat cccattgatt catagtgctt caccccgctt
     8101 tattcaatgt cgaatgtttc ttcaacggtt gcaacaaatt ccttaccata attattattc
     8161 ccttttatgt tgtctaaaat ataagtaagt tcttggtgga attcagtcaa ttctagttca
     8221 tcattgataa taattttttt agtcgtgttt tcatgcaaca tcgtccatgt ttcttgtacg
     8281 aaaatgagat aagcatacgc tttaccatca tctaatgtat acgcaaaggc ataattatca
     8341 ctatcaacga gcatttgtcc tacttcagat aatcctttaa tagaatcttc tgtataacaa
     8401 taaagtatgt catctttaat tttaatttca ttaatatata aacgcatgct taatcctcct
     8461 cttattccat aacattttaa cacaattcaa taatacaaaa cacttacgaa cactattttt
     8521 aatggtcaaa ttaacat
//
LOCUS       DAHHSC010000102         8505 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.103, whole genome shotgun sequence.
ACCESSION   DAHHSC010000102 DAHHSC010000000
VERSION     DAHHSC010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 8505)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8505)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8505
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.103"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            125..1654
                     /gene="aur"
                     /locus_tag="NBI57_001713"
     CDS             125..1654
                     /gene="aur"
                     /locus_tag="NBI57_001713"
                     /EC_number="3.4.24.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001225028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloproteinase aureolysin"
                     /protein_id="HCD1822652.1"
                     /translation="MRKFSRYAFTSMAAVTLLSTLSPAALAIDSKNKPANSDIKFEVT
                     QKSDAVKALKELPKSENVKNIYQDYAVTDVKTDKKGFTHYTLQPSVDGVHAPDKEVKV
                     HADKSGKVVLINGDTDAKKVKPTNKVTLSKDDAADKAFKAVKIDKHKAKNLKDKVIKE
                     NKVEIDGDSNKYVYNVELITVTPEISHWKVKIDAQTGEILEKMNLVKEAAETGKGKGV
                     LGDTKDININSIDGGFSLEDLTHQGKLSAFSFNDQTGQATLITNEDENFVKDEQRAGV
                     DANYYAKQTYDYYKDTFGRESYDNQGSPIVSLTHVNNYGGQDNRNNAAWIGDKMIYGD
                     GDGRTFTSLSGANDVVAHELTHGVTQETANLEYKDQSGALNESFSDVFGYFVDDEDFL
                     MGEDVYTPGKEGDALRSMSNPEQFGQPAHMKDYVFTEKDNGGVHTNSGIPNKAAYNVI
                     QAIGKSKSEQIYYRALTEYLTSNSNFKDCKDALYQAAKDLYDEQTAEQVYEAWNEVGV
                     E"
     gene            2021..2470
                     /locus_tag="NBI57_001714"
     CDS             2021..2470
                     /locus_tag="NBI57_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HCD1822653.1"
                     /translation="MKKSKRLEIVSTIVKKHKIYKKEQIISYIEEYFGVRYSATTIAK
                     DLKELNIYRVPIDCETWIYKAINNQTEQEMREKFRHYCEHEVLSSIINGSYIIVKTSP
                     GFAQGINYFIDQLNIEEILGTVSGNDTTLILTASNDMAEYVYAKLFK"
     gene            2473..2640
                     /locus_tag="NBI57_001715"
                     /pseudo
     CDS             2473..2640
                     /locus_tag="NBI57_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501420.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2817..4052
                     /gene="arcA"
                     /locus_tag="NBI57_001716"
     CDS             2817..4052
                     /gene="arcA"
                     /locus_tag="NBI57_001716"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HCD1822654.1"
                     /translation="MTDGPIKVNSEIGALKTVLLKRPGKELENLVPDYLDGLLFDDIP
                     YLEVAQKEHDHFAQVLREEGVEVLYLEKLAAESIENPQVRSEFIDDVLAESKKTILGH
                     EEEIKTLFATLSNQELVDKIMSGVRKEEINPKCTHLVEYMDDKYPFYLDPMPNLYFTR
                     DPQASIGHGITINRMFWRARRRESIFIQYIVKHHPRFKDANIPIWLDRDCPFNIEGGD
                     ELVLSKDVLAIGVSERTSAQAIEKLARRIFENPQATFKKVVAIEIPTSRTFMHLDTVF
                     TMIDYDKFTMHSAILKAEGNMNIFIIEYDDVNKDIAIKQSSHLKDTLEDVLGIDDIQF
                     IPTGNGDVIDGAREQWNDGSNTLCIRPGVVVTYDRNYVSNDLLRQKGIKVIEISGSEL
                     VRGRGGPRCMSQPLFREDI"
     gene            4085..5095
                     /gene="argF"
                     /locus_tag="NBI57_001717"
     CDS             4085..5095
                     /gene="argF"
                     /locus_tag="NBI57_001717"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HCD1822655.1"
                     /translation="MTEIQKPYDLKGRSLLKESDFTKAEFEGLIDFAITLKEYKKNGI
                     KHHYLSGKNIALLFEKNSTRTRAAFTVASIDLGAHPEFLGKNDIQLGKKESVEDTAKV
                     LGRMFDGIEFRGFSQQAVEDLAKFSGVPVWNGLTDDWHPTQMLADFMTIKENFGYLEG
                     INLTYVGDGRNNIAHSLMVAGAMLGVNVRICTPKSLNPKEAYVDIAKEKASQYGGSIM
                     ITDNIAEAVENTDAIYTDVWVSMGEESEFEQRINLLKDYQVNQQMFDLTGKDSTIFLH
                     CLPAFHDTNTLYGQEIYEKYGLAEMEVTDQIFRSEHSKVFDQAENRMHTIKAVMAATL
                     GS"
     gene            5186..6616
                     /gene="arcD"
                     /locus_tag="NBI57_001718"
     CDS             5186..6616
                     /gene="arcD"
                     /locus_tag="NBI57_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine-ornithine antiporter"
                     /protein_id="HCD1822656.1"
                     /translation="MNESGDNKLSKSSLIGLVIGSMIGGGAFNIMSDMGGKAGGLAII
                     IGWIITAIGMISLAFVFQNLTNERPELDGGIYSYAQAGFGDFVGFISAWGYWFSAFLG
                     NVAYATLLMSAVGNFFPIFKGGNTLPSIIVASLLLWGVHFLILKGVETAAFINSIVTV
                     AKLIPILLVIICMIIAFNFDTFKTGFFGMTSEGVLPFSWASTMSQVKSTMLVTVWVFI
                     GIEGAVIFSSRAKNKKDVGSATVIGLISVLIIYFLLTVLAQGVILQNHISQLDSPSMA
                     QVLATIVGGWGSTLVNIGLIISVLGAWLGWTLLAGELPFIVAKDGLFPKWFAKENKNG
                     APVNALLITNILVQLFLISMLFTQSAYQFAFSLASSAILYPYMFSAFYQVKYTLEHRQ
                     QATTKQWTIGIIASIYAIWLIYAAGINYLLLTMLLYIPALLVYSIVQKNNQTRLIKSD
                     YILFMIIIVLAVIGLIKLLMGTINVF"
     gene            6633..7574
                     /gene="arcC"
                     /locus_tag="NBI57_001719"
     CDS             6633..7574
                     /gene="arcC"
                     /locus_tag="NBI57_001719"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HCD1822657.1"
                     /translation="MKEKIVIALGGNAIQTKEATAEAQQTAIRRAMQNLKPLFDSPAR
                     IVISHGNGPQIGSLLIQQAKSNSDTTPAMPLDTCGAMSQGMIGYWLETEINRILTEMN
                     SDRTVGTIVTRVEVDKDDPRFNNPTKPIGPFYTKEEVEELQKEQPDSVFKEDAGRGYR
                     KVVASPLPQSILEHQLIRTLADGKNIVIACGGGGIPVIKKENTYEGVEAVIDKDFASE
                     KLATLIEADTLMILTNVENVFINFNEPNQQQIDDIDVATLKKYAAQGKFAEGSMLPKI
                     EAAIRFVESGENKKVIITNLEQAYEALIGNKGTHIHM"
     gene            7673..8377
                     /locus_tag="NBI57_001720"
     CDS             7673..8377
                     /locus_tag="NBI57_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HCD1822658.1"
                     /translation="MTENFILGRNNKLEHELKALADYINIPYSILQPYQSECFVRHYT
                     KGQVIYFSPQESSNIYFLIEGNIIREHYNQNGDVYRYFNKEQVLFPISNLFHPKEVNE
                     LCTALTDCTVLGLPRELMAFLCKANDDIFLTLFALINDNEQQHMNYNMALTSKFAKDR
                     IIKLLCHLCQTVGYDQDEFYEIKQFLTIQLMSDMAGISRETAGHIIHELKDEKLVVKD
                     HKNWLVSKHLFNDVCV"
BASE COUNT         3116 a         1182 c         1640 g         2567 t
ORIGIN      
        1 atcaataaat aaaatcaacg aacaaaaagt atcgaaataa atagaaataa tcatttactt
       61 ttctgaaaaa ttaaattaat attttattta taagctgttt ttaagatttc aggaggaatg
      121 aaatgtgagg aaattttcaa gatatgcatt tacaagtatg gcagcagtaa cattgttgag
      181 cactttatca ccagcagcat tagcgattga ttcaaaaaat aaaccagcta attctgatat
      241 taaatttgag gtgactcaaa agagtgatgc ggtcaaagca ttaaaagaat tgcctaaatc
      301 cgaaaatgta aaaaatattt atcaagatta cgctgttact gatgtaaaaa ctgataaaaa
      361 aggatttacg cattatacat tgcaaccgag tgttgatggt gttcatgcac ctgacaaaga
      421 agtgaaagta cacgcagaca aatcaggaaa agtcgtttta atcaatgggg atactgatgc
      481 gaagaaagta aagccaacga ataaagtgac attaagtaaa gatgacgcag ccgacaaagc
      541 atttaaagca gttaagattg ataagcataa agcgaaaaat cttaaagata aagtcattaa
      601 agaaaacaaa gttgaaatcg atggtgacag taataaatac gtttataatg ttgagttaat
      661 tacagtgaca ccagaaattt cacattggaa agttaaaatt gatgctcaaa ctggcgaaat
      721 tttagaaaaa atgaacttag ttaaagaagc tgcagaaact ggtaaaggaa aaggtgtact
      781 tggcgataca aaagatatca atatcaatag tattgacggt ggatttagcc tagaagattt
      841 aacgcatcaa ggtaaattat cagcatttag ctttaatgat caaacaggtc aagcaacatt
      901 gattactaat gaagatgaaa acttcgtaaa agatgagcaa cgtgctggcg tagatgcaaa
      961 ttattacgct aaacaaacat atgattatta caaagacaca tttggtcgtg aatcatatga
     1021 caaccaaggt agtccaattg tttcattaac gcatgttaat aactacggtg gtcaagataa
     1081 cagaaataat gccgcatgga ttggtgacaa aatgatctat ggtgatggtg atggtcgcac
     1141 attcacaagt ttatcgggtg caaatgacgt agtggcacac gaattaacac acggtgtgac
     1201 acaagagact gcgaacttag aatataagga ccagtcaggc gctctaaatg aaagcttttc
     1261 agatgttttt ggatactttg tagatgacga ggatttctta atgggtgaag atgtctacac
     1321 acctggaaaa gagggggacg ctttacgcag catgtcaaac ccagaacaat ttggtcaacc
     1381 agctcatatg aaagactatg tattcactga aaaagataat ggtggcgtac atacgaattc
     1441 tggaattcca aataaagcag cttataacgt gattcaagca atagggaaat ctaaatcaga
     1501 acaaatttac taccgagcat taacggaata cttaacaagt aattcaaact tcaaagattg
     1561 taaagatgca ttataccaag cggctaaaga tttatatgac gagcaaacag ctgaacaggt
     1621 gtatgaagca tggaatgaag taggcgtgga gtaaaaatat ataaacaaga agaagtaatg
     1681 ttaaacactt ataaataatt aaattttaaa tacagcttat atatggggta tacctacatt
     1741 gataattgac tttgtaggta taccctctct ttgtaattag atgagaggag tgtgagggtt
     1801 gtctgccgaa agactactcg gcagtctaaa atcattacaa gtagtagata tgtgataatt
     1861 aaatgctgac ttagaataca aaattcattt taaaagttgt cacaaaaaat ttacatgtat
     1921 ttttattatc ttttgcaaaa caaagtgtta aattataaat gaaacatgca tgaatttatt
     1981 ttttaataca agaaacgtaa ctaccaaagg agtttacaat atgaagaaaa gtaaacgatt
     2041 agaaattgtt tctacaatag ttaaaaagca taagatttat aaaaaagaac aaatcatttc
     2101 atatattgaa gaatattttg gtgtaagata tagtgcaaca acaattgcta aagacttgaa
     2161 agaacttaat atatatcgtg tccctatcga ttgtgaaaca tggatttata aagctattaa
     2221 taatcaaaca gaacaagaga tgagagaaaa gtttagacac tattgtgaac atgaagttct
     2281 aagttcaatc atcaatggtt catacattat cgttaaaacc tcacctggtt tcgcccaagg
     2341 cataaactat tttatcgatc agctaaatat agaagagata ttaggtacgg tgagtggaaa
     2401 tgacactaca ttaatcttaa ctgcctcaaa tgatatggca gaatacgtat atgcaaaatt
     2461 atttaaatag acatgtatca aatgaataat aaaaattagt ttcgtatcac gtgtacacaa
     2521 gttagttacc aaatattaac ttgtgtacgc gtttttttat ggaaagaaag aattcatagt
     2581 cattcatttg actgtataaa aaactttata caacgtgttt ttgtgggtct ttttgaataa
     2641 aaaatgtata ttttgaccca aaacaccttt atttatatat aaaaatccat tattatgtat
     2701 tgtataacaa aaagatatga aattttcgac tttctttatg tgaatataat cacatgtaag
     2761 cgtttgaaga ttgtctatac tctaaatgaa ttcaaagata aaaggaggaa atagacatga
     2821 cagatggtcc aattaaagta aatagcgaaa ttggagcttt aaaaactgtg ttacttaagc
     2881 gtcctggaaa agaattagaa aatttagtac ctgattattt agatggatta ctatttgatg
     2941 atattccata tttagaagta gctcaaaaag agcatgacca ttttgcgcag gtgctaagag
     3001 aagagggtgt tgaagtactt taccttgaga agttagcagc tgaaagtatt gaaaatcctc
     3061 aagtaagaag tgaatttatt gatgatgtat tagcagagtc taaaaaaaca atattaggtc
     3121 atgaagaaga aattaagaca ttatttgcga cactttctaa tcaagaactt gtagataaaa
     3181 taatgtcagg ggtacgtaag gaagaaatta atccgaaatg tacacatcta gtagagtaca
     3241 tggatgataa gtatccattc tatttagatc caatgccaaa cctttatttt actagagatc
     3301 cacaagcctc aataggacac ggtataacaa tcaatcggat gttctggaga gcacgacgac
     3361 gagaatcaat atttattcaa tatattgtaa agcatcatcc tagatttaaa gatgcgaata
     3421 ttccaatctg gttagatcga gattgcccat tcaatattga aggcggcgat gaacttgttt
     3481 tatctaaaga tgtcttggct ataggcgttt cagaacgtac atctgcacaa gctattgaaa
     3541 agttagcgcg acgtattttt gaaaatccgc aggcgacgtt taaaaaagta gtagcaattg
     3601 aaattccaac tagtcgaact tttatgcact tagatacagt atttacaatg atagattatg
     3661 acaaatttac aatgcattca gccattttaa aggcagaagg caatatgaat atatttatta
     3721 ttgaatatga tgacgtaaat aaagatattg ccatcaaaca atctagtcat ttaaaagata
     3781 ctttagaaga cgtactaggt atagatgata tccaattcat tccaacagga aatggtgatg
     3841 tcattgatgg tgctagagag caatggaatg atggctcaaa tacattatgt ataagaccag
     3901 gcgttgtagt gacttacgat agaaactatg tatcgaatga tttattgaga caaaaaggca
     3961 taaaagtcat tgaaatatct ggtagcgagc tggtacgtgg acgtgggggc cctagatgta
     4021 tgagtcaacc actattcaga gaagacattt aacgtaataa attatagaag cgagggtgaa
     4081 aataatgaca gaaattcaaa aaccgtatga tttaaaaggc agatcattat taaaagaaag
     4141 tgattttacc aaagcagaat tcgaaggact tattgatttt gcaattacat taaaagagta
     4201 taagaaaaac ggtattaagc atcactactt atctggaaaa aatattgcac tactattcga
     4261 aaagaattcg acgagaacgc gtgcagcgtt tacagttgcg tctattgatt taggtgcgca
     4321 tccagaattt ttagggaaaa atgatattca attaggcaaa aaagaatctg tagaggatac
     4381 tgcgaaagta ttaggtagaa tgttcgatgg tattgaattc cgtggttttt cacaacaagc
     4441 tgttgaagat ttagcgaagt tctctggtgt accggtgtgg aatggattaa cagacgattg
     4501 gcatcctaca caaatgttag ctgattttat gacaataaaa gagaattttg gatatctaga
     4561 aggaataaac ttaacttacg ttggagatgg acgtaataat attgcgcatt cattaatggt
     4621 agcaggtgct atgttaggtg ttaatgtaag aatttgtaca cctaaatcat taaatccaaa
     4681 agaggcatat gttgatattg caaaagaaaa agcgagtcaa tatggtggtt caatcatgat
     4741 tacggataat attgcagaag cagttgaaaa tacagatgct atatatacag atgtttgggt
     4801 atcgatgggt gaagaaagtg aatttgaaca acgtattaat ttattaaaag actatcaagt
     4861 gaatcaacag atgtttgatt taacaggtaa agattcaacg atattcttac attgtttacc
     4921 agcattccat gatacaaata cactttatgg acaagaaatt tatgaaaaat atggattagc
     4981 tgaaatggaa gttacagacc aaatctttag aagtgaacat tcaaaagtgt ttgatcaagc
     5041 tgaaaataga atgcatacaa ttaaggcagt aatggcagca acattgggga gttaatcact
     5101 aaatggaacg atatgaatat gatgtgtctg atgatataag tgtcatgtac agacacctca
     5161 tattggtatt aaaggagaaa tgaatatgaa cgaatcagga gataacaaac tcagtaaatc
     5221 ttctttaatt ggactagtta taggatccat gattggtggc ggtgcgttca atataatgtc
     5281 tgatatgggc ggtaaagccg gtggattagc cattattatt ggttggatta ttacagctat
     5341 aggaatgatt tcattagcgt tcgtatttca aaatttaacc aatgaacggc cggagctaga
     5401 cggtggtatt tatagttatg ctcaagcagg atttggcgat tttgtaggat ttatcagtgc
     5461 ttggggatat tggttctcag cgtttttagg caatgttgcc tatgcaacac tattgatgtc
     5521 agcagtaggt aactttttcc cgatttttaa aggaggcaac acattaccaa gtattattgt
     5581 cgcctcgtta ctactctggg gtgtccattt cttgatttta aaaggcgttg aaacagcagc
     5641 atttatcaat agtattgtta ctgttgcaaa gttaatcccg attttacttg taatcatatg
     5701 catgataatt gcattcaatt ttgacacttt taaaacaggc tttttcggta tgacgtcaga
     5761 gggtgtattg ccatttagtt gggcgagcac aatgagccaa gttaaaagta cgatgctagt
     5821 gacagtttgg gtgtttatcg gtatcgaagg tgcagtaatt ttttctagta gagctaaaaa
     5881 taaaaaagat gtaggtagtg ccacggttat aggacttata tcagttttaa ttatctattt
     5941 cttattaact gtattagctc aaggcgtgat tttgcaaaat catatttctc aattagattc
     6001 gccaagtatg gcacaggtgc ttgcaactat tgtaggtggt tggggatcta cacttgtaaa
     6061 tattggttta attatttcgg tactaggtgc atggttagga tggacattgc ttgctggtga
     6121 attacctttc attgttgcaa aagatggatt atttccaaag tggtttgcta aagaaaataa
     6181 aaatggagca cctgtaaatg cactgcttat taccaatata ttagtacaat tatttttaat
     6241 aagtatgcta tttacacaga gtgcgtatca atttgcattt tcactagcat caagtgctat
     6301 tttataccct tacatgttca gtgcatttta ccaagttaaa tacactttag agcatcgaca
     6361 gcaagcaact actaaacaat ggacgattgg catcatagcc tcaatttatg ctatatggct
     6421 tatatatgca gcaggtatca attacttatt attgacgatg ttactttata ttccagctct
     6481 tcttgtttat tcaatcgttc aaaagaataa tcagacacgt ttgattaaat cagactatat
     6541 tctttttatg attattatcg tacttgcagt tatcggatta attaagttat tgatgggaac
     6601 gataaatgtt ttttaaaagg agcgacaaaa atatgaaaga gaaaattgtc attgcattag
     6661 gcggtaatgc gatacagaca aaagaagcaa cagctgaagc acaacaaaca gctattagac
     6721 gtgcgatgca aaaccttaaa cctttatttg attcaccagc gcgtattgtc atttcacatg
     6781 gtaatggccc acaaattgga agtttattaa tccaacaagc taaatcgaac agtgacacaa
     6841 cgccggcaat gccattggat acttgtggtg caatgtcaca gggtatgata ggctattggt
     6901 tggaaactga aatcaatcgc attttaactg aaatgaatag tgatagaact gtaggcacaa
     6961 tcgttacacg tgtggaagta gataaagatg atccacgatt caataaccca accaaaccaa
     7021 ttggtccttt ttatacgaaa gaagaagttg aagaattaca aaaagaacag ccagactcag
     7081 tctttaaaga agatgcagga cgtggttata gaaaagtagt tgcgtcacca ctacctcaat
     7141 ctatactaga acaccagtta attcgaactt tagcagacgg taaaaatatt gtcattgcat
     7201 gcggtggtgg cggtattcca gttataaaaa aagaaaatac ctatgaaggt gttgaagcgg
     7261 ttatagataa agattttgct agtgagaaat tagcaacgct gattgaagca gataccttaa
     7321 tgattcttac gaatgtagaa aatgtattta ttaactttaa tgaacctaat caacaacaaa
     7381 tcgatgatat tgatgtagca acactgaaaa aatacgcggc acaaggtaag tttgcggaag
     7441 gatcgatgtt gccaaaaata gaagctgcga tacgatttgt tgaaagtggg gaaaacaaaa
     7501 aagttatcat taccaattta gagcaggcat acgaagcttt gattggtaat aaaggtacac
     7561 acattcacat gtagcacttt atcacgcgac aaaacattaa atatgtttct ccgttgattc
     7621 aaatgaaaaa gttgtctgct gacactttgc aaggtttgaa ggagtttaac ttatgacaga
     7681 aaactttatt ttgggtagaa ataataaatt agaacatgaa ctaaaggcat tagcagatta
     7741 cattaatatc ccatatagta tattacaacc atatcaaagt gaatgttttg tcagacatta
     7801 tacgaaaggc caagttattt atttttcgcc acaagaaagt agcaatattt actttttaat
     7861 tgaaggtaat attattagag aacattacaa tcaaaatgga gatgtatatc gttattttaa
     7921 taaagagcaa gtattatttc caatcagtaa cttatttcat ccgaaagagg ttaacgaatt
     7981 gtgtacagca ttaaccgatt gtacagttct tggattgcct agagaattga tggctttttt
     8041 gtgcaaagct aatgatgata tatttttgac actttttgca ttaataaatg ataatgagca
     8101 gcaacacatg aactataaca tggcattaac aagtaaattt gctaaagatc gaattattaa
     8161 attgttatgt catctatgtc agacagtagg gtacgatcaa gatgaatttt atgaaatcaa
     8221 acagttttta actattcaac tcatgagtga tatggctggg atttcccggg aaacagctgg
     8281 tcatattatt catgaactta aagatgaaaa acttgttgtt aaagatcata aaaattggtt
     8341 agtaagcaaa catttattca atgatgtatg tgtttaatat acaatgtaaa atgaataagt
     8401 tgaacatgag gtctaacgta catttatacg ttaggccttt tttgctagca tgatgaataa
     8461 tttaaaatgt tagttaaatt tgattgttga aattacagta aaatt
//
LOCUS       DAHHSC010000103         8378 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.104, whole genome shotgun sequence.
ACCESSION   DAHHSC010000103 DAHHSC010000000
VERSION     DAHHSC010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 8378)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8378)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8378
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.104"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            <1..382
                     /locus_tag="NBI57_001721"
     CDS             <1..382
                     /locus_tag="NBI57_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YisL family protein"
                     /protein_id="HCD1822659.1"
                     /translation="LHILSWVLAIILFIATYLNISKNQGGSPFFKPLHMILRLFMLLT
                     LISGFWILIQSFMNGGANHMLLTLKMLCGVAVVGLMEVSIAKRKRHEQSHKMFWITMA
                     LIIITMVLGVILPLGPISKLFGIG"
     gene            complement(554..1870)
                     /locus_tag="NBI57_001722"
     CDS             complement(554..1870)
                     /locus_tag="NBI57_001722"
                     /EC_number="1.8.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-disulfide reductase"
                     /protein_id="HCD1822660.1"
                     /translation="MPKIVVVGAVAGGATCASQIRRLDKESDIIIFEKDRDMSFANCA
                     LPYVIGEVVEDRKYALAYTPEKFYDRKQITVKTYHEVIAINDERQTVTVLNRKTNEQF
                     EESYDKLILSPGASANSLGFESDITFTLRNLEDTDAIDQFIKANQVDKVLVIGAGYVS
                     LEVLENLYERGLHPTLIHRSDKINKLMDADMNQPILDELDKREIPYRLNEEIDAINGN
                     EITFKSGKVEHYDMIIEGVGTHPNSKFIESSNIKLDRKGFIPVNDKFETNVPNIYAIG
                     DIATSHYRHVDLPASVPLAWGAHRAASIVAEQIAGNDTIEFKGFLGNNIVKFFDYTFA
                     SVGVKPNELKQFDYKMVEVTQGAHANYYPGNSPLHLRVYYDTSNRQILRAAAVGKEGA
                     DKRIDVLSMAMMNQLTVDELTEFEVAYAPPYSHPKDLINMIGYKAK"
     gene            complement(1922..2746)
                     /locus_tag="NBI57_001723"
     CDS             complement(1922..2746)
                     /locus_tag="NBI57_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HCD1822661.1"
                     /translation="MQPHLICLDLDGTLLNDNKEISSYTKQVLNELQQRGHQIMIATG
                     RPYRASQMYYHELNLTTPIVNFNGAYVHHPKDKNFKTCHEILDLGIAQNIIQGLQQYQ
                     VSNIIAEVKDYVFINNHDPRLFEGFSMGNPRIQTGNLLVHLKESPTSILIEAEESKIP
                     EIKNMLTHFYADHIEHRRWGAPFPVIEIVKLGINKARGIEQVRQFLNIDRNNIIAFGD
                     EDNDIEMIEYARHGVAMENGLQELKDVANNITFNNNEDGIGRYLNDFFNLNIRYYC"
     gene            2860..3168
                     /locus_tag="NBI57_001724"
     CDS             2860..3168
                     /locus_tag="NBI57_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002465312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HCD1822662.1"
                     /translation="MEEALKDSILGALEMVIDPELGIDIVNLGLVYKVNVDDEGVCTV
                     DMTLTSMGCPMGPQIIDQVKTVLAEIPEIQDTEVNIVWSPPWTKDMMSRYAKIALGVS
                     "
     gene            3716..5530
                     /locus_tag="NBI57_001725"
     CDS             3716..5530
                     /locus_tag="NBI57_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HCD1822663.1"
                     /translation="MNKTKGFTKYKKMRYMPGLDGLRAIAVLGIIIYHLNKQWLTGGF
                     LGVDTFFVISGYLITSLLLKEYDDTGIIKLKSFWIRRLKRLLPAVIVLLMVVGTATLL
                     LKSDNIIRVKHDIIAAIFYVSNWWYIAKDVNYFEQFSFMPLKHLWSLAIEEQFYIFFP
                     VILVTLLLTIKKRYKIGFIFWGVSIISLGLMMFIYSINGDHSRVYFGTDTRLQTLLLG
                     VILAFLWPPFKLKNDPPKVVKYVIDSIGSLSFIVLILLFFIINDETNWIYDGGFYLIS
                     ILTLFIIASVVHPSTWIAKIFSNPVLVFIGKRSYSLYLWHFAVISFVHSYYVDGQIPV
                     YVYFIDISLTIIFAELSYRFIETPFRKEGIKALNWRPSYIPQFIRMAIVVTLLIPFML
                     ILVGAFNKYGKDIIGEKANSFDTTIEDNYLMRIAPIDNIHIDGLVSEKKKESSDVYNN
                     IKPLLIGDSVMVDIGESFKSSVPKSRIDGKVGRQLYQTLPLVKANYSQYKKSSDQVVL
                     ELGTNGDFTVKQLDDLLNQFGKAKIYLVNTRVPRIYEANVNRLLADAAKRKSNVTLID
                     WNKRSQGHSEYFAPDGVHLEYKGVLALKDEILKALKKK"
     gene            5733..8342
                     /gene="clpB"
                     /locus_tag="NBI57_001726"
     CDS             5733..8342
                     /gene="clpB"
                     /locus_tag="NBI57_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HCD1822664.1"
                     /translation="MDINKMTYAVQSALQQAVELSQQHKLQNIEIEAILSAALNESES
                     LYKSILERANIEVDQLNKAYEDKLNTYASVEGDNIQYGQYISQQANQLITKAESYMKE
                     YEDEYISMEHILRSAMDIDQTTKHYINNKVEVIKEIIKKVRGGNHVTSQNPEVNYEAL
                     AKYGRDLVEEVRQGKMDPVIGRDEEIRNTIRILSRKTKNNPVLIGEPGVGKTAIVEGL
                     AQRIVKKDVPESLLDKTVFELDLSALVAGAKYRGEFEERLKAVLKEVKESDGRIILFI
                     DEIHMLVGAGKTDGAMDAGNMLKPMLARGELHCIGATTLNEYREYIEKDSALERRFQK
                     VAVSEPDVEDTISILRGLKERYEVYHGVRIQDRALVAAAELSDRYITDRFLPDKAIDL
                     VDQACATIRTEMGSNPTELDQVNRRVMQLEIEESALKNESDNASKQRLQELQEELANE
                     KEKQAALQSRVESEKEKIANLQEKRAQLDESRQALEDAQTNNNLEKAAELQYGTIPQL
                     EKELRELEDNFQDEQGEDTDRMIREVVTDEEIGDIVSQWTGIPVSKLVETEREKLLHL
                     SDILHKRVVGQDKAVDLVSDAVVRARAGIKDPNRPIGSFLFLGPTGVGKTELAKSLAA
                     SLFDSEKHMIRIDMSEYMEKHAVSRLIGAPPGYIGHDEGGQLTEAVRRNPYSVILLDE
                     VEKAHTDVFNVLLQILDEGRLTDSKGRSVDFKNTIIIMTSNIGSQVLLENVKETGEIT
                     ESTEKAVMTNLNAYFKPEILNRMDDIVLFKPLSIDDMSMIVDKILTQLNIRLLEQRIS
                     IEVSDDAKAWLGQEAYEPQYGARPLKRFVQRQIETPLARMMIKEGFPEGTTIKVNLNS
                     DNNLTFNVEKIHE"
BASE COUNT         2908 a         1198 c         1508 g         2764 t
ORIGIN      
        1 tttacatata ttaagttggg tattagcgat tattttattt atcgctacat acttaaacat
       61 ttcaaaaaat caaggcggat caccattttt caaaccgttg cacatgattt tacgcttatt
      121 tatgctgttg acgttaattt caggattttg gatattaatt cagtcattta tgaatggcgg
      181 ggcaaatcat atgttgctta cattgaaaat gctgtgtggt gttgcagtag ttggattgat
      241 ggaagtgtcg attgctaaaa gaaagagaca tgaacaaagt cacaaaatgt tttggataac
      301 aatggcatta attatcatca caatggtatt aggtgtcatt ctaccgttag ggcctatatc
      361 aaaattattc ggtattggct aattgataag acatatagta tatgacataa ggcatggaca
      421 attattagtc caagacctta tgtctttttt gattctaaaa atgtttaaac ataaaaaaag
      481 gactggatag tagtaccagt ccaacataat tctaacctag acaattaaga ttagattgaa
      541 tcgcatgatt catttattta gctttgtaac caatcatatt gattaaatct ttagggtggc
      601 tatatggtgg tgcataagcc acttcaaact cagttaactc atctacagtt agctggttca
      661 tcattgccat cgatagtaca tcaatacgtt tatctgcacc ttcttttcct actgcagctg
      721 ctcttaaaat ctgacggttt gaagtgtcat aatataccct taagtgtaaa ggggaatttc
      781 ctgggtaata attcgcgtgt gcaccttgag tgacttccac cattttatag tcaaattgct
      841 ttagttcatt tggtttaacg ccgacactcg caaatgtata atcaaagaac ttcacaatat
      901 tgttgcctaa gaagcctttg aattcaatag tgtcatttcc agcaatttgt tcggcaacaa
      961 tacttgctgc acggtgagcg ccccaagcta aaggaacact agccggtaga tcgacatgtc
     1021 gataatgtga tgttgcaata tcgcctattg cataaatgtt tggaacattt gtttcaaatt
     1081 tatcgtttac cggtatgaaa ccttttcgat caagtttgat atttgaactt tcgataaatt
     1141 ttgaattggg gtgagtaccg acaccttcaa taatcatatc gtaatgttca acttttcctg
     1201 atttaaatgt aatttcattt ccattgatag catcaatttc ctcatttaaa cggtatggaa
     1261 tctcccgctt atctaattca tcaagtatag gttgattcat gtcagcatcc attaatttat
     1321 ttatcttatc agatcgatga attaaagtag ggtgtaaacc acgttcgtaa agattttcaa
     1381 gaacttctaa tgaaacatac cctgcaccta taaccaatac tttatcaact tgatttgctt
     1441 tgatgaattg atcgatagca tcagtgtctt ctaaatttct aagtgtaaat gtaatatcac
     1501 tttcaaagcc aaggctattt gcacttgcac cagggcttaa aatgagttta tcgtaagatt
     1561 cttcaaattg ttcgttggtc tttctattta atacagttac agtttgtctt tcatcattga
     1621 ttgcaataac ttcatgataa gtttttactg taatttgctt tctatcataa aatttttcag
     1681 gtgtatacgc taaagcatat tttctatctt caacaacttc gccaatgaca taaggcaatg
     1741 cacaattagc aaagctcata tcacgatctt tttcaaaaat aataatgtca ctttctttat
     1801 ctaaacgtcg aatttggctg gcacatgttg caccgccagc gactgctccg actacgacta
     1861 ttttgggcat cgtaacttac ctcctattgt tttaaaatat tataaaatga ttagttataa
     1921 attaacagta atatctaata tttaaattaa agaaatcatt caaatatcga ccaatgccat
     1981 cttcattatt gttgaatgta atattgttcg ctacatcttt aagttcttgc aaaccatttt
     2041 ccatagcaac accatggcgg gcgtactcaa tcatttcaat atcattatct tcatcaccga
     2101 atgcaataat attatttcgg tcaatattta aaaattgtct aacttgctca atgcctcttg
     2161 ctttattaat accaagtttt acaatttcaa tgacagggaa tggtgcgccc cagcgtcgat
     2221 gctcaatatg atcggcataa aaatgagtaa gcatattttt gatttcaggt attttacttt
     2281 cttcggcttc aattaaaatt gaggtagggg attctttcaa gtggacaagt aaattaccag
     2341 tttgaattct tggattaccc attgaaaaac cttcaaataa tcttggatca tgattgttaa
     2401 tgaaaacata atctttcact tctgctataa tattcgatac ttgatattgt tgtaatcctt
     2461 gaataatgtt ttgtgcgatg cctaaatcta aaatttcatg gcaagttttg aagtttttat
     2521 ctttagggtg atgtacgtaa gcgccattaa aattaacaat tggtgtcgtt aaatttaatt
     2581 catgataata catttgactt gcacgataag gtctgccagt cgcaatcata atttggtgtc
     2641 cacgttgttg taattcattt aatacttgtt tagtatatga tgaaatttct ttgttatcgt
     2701 ttaataatgt tccgtctaag tctagacata ttaaatgtgg ttgcatatct ctctcctcat
     2761 aagttgctta gttcatatag atacatcata gcattttatt ggcgttttgt cggatatttg
     2821 atatattatg aataatatga cattaaagag gtgatatgga tggaagaggc attgaaagat
     2881 agtatcttag gtgcattaga aatggtaatt gaccctgaat taggaattga tatcgttaat
     2941 ttaggtttag tatacaaagt gaatgttgat gatgaaggcg tatgtacagt tgatatgact
     3001 ttaacatcaa tgggatgtcc aatgggacct caaattattg atcaagttaa aacagtatta
     3061 gcagagattc ctgaaattca ggatactgaa gtgaatatcg tatggagtcc accttggaca
     3121 aaagatatga tgtcacgtta cgctaagatt gcacttggtg tgagctaaca taaacgtgca
     3181 ttgcctgtag aatttctttt agaaattctc tgtgttgggt ccccgcagtc aatataacat
     3241 tgtagagtat aattcattgt gaataagctc ccttcaaagt agacattgaa aaatgaaaat
     3301 tttgaaggga gccttattta tgttctaggt tctgatttca ttttgaaaat ttatatctaa
     3361 cttgtttcaa aaaatatgaa ttaagacagt gacttgcact tccaagagtg cacaattcat
     3421 tttctagttg aatattttct tgaaaatttt tataataaat tgaatttata ttacagttat
     3481 attacaatac ataatacttc atagttttta agttgccgag ttgcattaca aattggattt
     3541 cgaagtaaga atcatcattg gtaatttttt taatttaaca gtattttagt caatatgatt
     3601 ttgatgatgt cgtaattcaa taatatacat atgcatttca atattctttt aaacagcata
     3661 actaaattta attaagcatc taagaagaac agttttaaaa gggaagaatg caaaaatgaa
     3721 caaaacaaag ggttttacaa agtataagaa aatgagatat atgccagggc tcgatggttt
     3781 gagggcaatc gctgttctag gaattattat ttaccactta aacaagcaat ggttgacagg
     3841 tggcttttta ggtgtggata cattttttgt gatctctggt tatttaatta caagcttatt
     3901 actcaaagag tatgatgaca caggtatcat taaattgaaa agcttttgga tacgtcgttt
     3961 aaaacgttta ttaccagcag tcatagtttt attaatggtt gtagggacag caaccttatt
     4021 attaaaatca gataatatca ttagggttaa acatgatatt attgctgcga tattttatgt
     4081 atcaaactgg tggtatatag caaaagatgt taattatttt gagcaatttt catttatgcc
     4141 attaaagcat ttatggtctt tagcaattga agaacagttt tacatatttt tcccagttat
     4201 tttggttaca ttattgttaa caattaaaaa gcgatacaaa ataggattta ttttttgggg
     4261 agtatcaata atttctttag ggttaatgat gtttatctac agtattaatg gggatcattc
     4321 acgagtgtat tttggtacag atactagatt acagacattg ttactgggtg ttattttagc
     4381 ttttttatgg ccaccgttta aattgaaaaa tgatccacct aaagttgtaa aatatgttat
     4441 tgatagcata ggtagtttat catttatagt acttatatta ttatttttca ttattaatga
     4501 tgagacgaat tggatatatg atggtggttt ctatttaata tccatattaa cgttatttat
     4561 tattgccagt gtcgttcatc catctacatg gatagcgaag atattttcaa atccagtgtt
     4621 agtatttatc gggaaaaggt cttatagttt atatttatgg cattttgcag taattagttt
     4681 cgtacatagt tactatgtag atggacagat acctgtatat gtgtacttta tagatataag
     4741 tttaacaatt atatttgcag agctatcata tcgctttata gaaactccat ttagaaaaga
     4801 aggtattaaa gctttaaatt ggcgaccttc ttatatacca caatttataa gaatggcaat
     4861 tgtagtaacc ttgttaattc catttatgtt gattttagta ggtgcattca ataaatatgg
     4921 taaagacatt attggagaaa aagcgaatag ctttgatacc actattgaag ataattattt
     4981 aatgcggata gcaccaattg ataacattca cattgatggc ttagtaagtg agaagaaaaa
     5041 ggaatcttcc gacgtatata ataatattaa acctctttta atcggtgatt cagtaatggt
     5101 tgatatcggt gagtcattta agtcatcagt tcctaagtct agaattgatg gaaaagtagg
     5161 gcgtcaattg tatcaaacct tacctttagt taaagcgaat tattcacaat ataaaaaatc
     5221 atctgatcaa gtcgtattag aattaggtac aaatggcgac tttactgtca aacagctcga
     5281 cgatttactt aatcaatttg gaaaagccaa gatttattta gttaatacac gtgttccaag
     5341 aatttatgag gcaaatgtaa atcgattatt agctgacgcg gcgaaacgaa agtccaatgt
     5401 cacattaatt gattggaata agcgatcaca aggacatagt gaatattttg caccagacgg
     5461 tgtacattta gagtacaaag gagtcctagc tttaaaagat gaaatattaa aagcacttaa
     5521 aaagaaataa catatgttta ataagtctag ttaatgtgta acgtaacatt agctagattt
     5581 ttttattcaa aaaaatattt acaaatatta ggaaatttaa gtgtaaaaga gttgataaat
     5641 gattatattg ggactataat ataattaagg tcaaagaaag tcaaagtcaa aagtgcaact
     5701 tgaccaactc tacaaattag aggtgaaatt atatggatat aaataaaatg acatatgctg
     5761 ttcaaagtgc tttacaacaa gcagttgaac tgagtcagca acataaatta caaaatatag
     5821 aaattgaggc aattttaagc gctgccttaa atgaaagtga aagcttatat aaaagtattt
     5881 tagaacgagc aaatattgag gtagatcaat taaacaaagc ttatgaagac aaactaaaca
     5941 cgtatgcatc tgtagaaggt gacaatatac aatatggtca atatattagc caacaagcaa
     6001 accaattgat aactaaggct gaatcataca tgaaagaata tgaagatgaa tatatttcaa
     6061 tggagcatat tttacgttcg gcaatggaca ttgatcaaac aacaaaacat tatataaata
     6121 ataaagtaga agttatcaaa gaaattatta aaaaagtaag agggggaaat cacgtgacat
     6181 cacaaaatcc agaagttaat tacgaagcat tagctaaata tggccgcgac ttagtagaag
     6241 aagttagaca aggtaaaatg gatcctgtta taggaagaga tgaagaaatt cgaaatacga
     6301 ttcgtatttt aagtcgtaaa actaaaaaca accctgtgct cattggtgaa ccaggtgttg
     6361 gtaaaactgc aattgttgaa ggattagcgc aacgtatagt taagaaagat gtgccagaat
     6421 cattattaga taaaactgtt tttgagttag atttaagcgc attagtagcg ggcgctaaat
     6481 atcgtggtga atttgaagag agattaaaag cagtcctaaa agaagttaaa gagtctgatg
     6541 gtagaattat attatttatt gatgaaatcc atatgcttgt aggtgctggt aaaacagatg
     6601 gtgccatgga tgcaggcaac atgctaaaac caatgttagc acgaggagag ttacattgta
     6661 ttggtgcaac aactttaaat gaatatcgag aatatattga aaaagattcg gcattagagc
     6721 gtcgtttcca aaaagtagca gttagtgagc ctgatgttga agatacaatt tcaattttac
     6781 gtggtttaaa agaacgatat gaagtgtatc atggtgtgcg tattcaagat agagccttag
     6841 ttgctgccgc tgaattgtct gatcgttaca tcactgatcg ttttttacca gataaagcga
     6901 ttgatttagt tgaccaagca tgtgcaacaa ttcgtacgga aatgggatca aatccaactg
     6961 aattggatca agttaataga cgtgtcatgc aattagaaat tgaagaaagc gcacttaaaa
     7021 atgaatctga caatgcgagc aaacagagat tacaagaact acaagaagag cttgccaatg
     7081 aaaaagagaa acaagcagca cttcaatctc gtgtagaatc agaaaaagaa aaaatagcaa
     7141 atttacaaga aaaacgtgcg caactagatg aaagtagaca agcgttggaa gatgcacaaa
     7201 caaataacaa tttagaaaaa gctgctgaac tacaatatgg aacaattcct caattggaaa
     7261 aagaacttag agaattagag gataatttcc aagatgagca aggtgaagat acagatcgaa
     7321 tgattcgtga agttgtaaca gacgaagaaa ttggcgatat tgtcagccaa tggacaggca
     7381 taccagtttc aaaattagtt gaaacagaac gtgaaaaatt acttcactta agtgacatct
     7441 tgcataaacg tgttgtaggt caagataaag cggttgacct ggtttcagat gcagtagtta
     7501 gagcaagagc aggtattaaa gatccaaaca gacctattgg tagtttctta ttcctaggtc
     7561 caactggagt aggtaaaact gaattagcta aatcattagc tgcatcatta tttgattctg
     7621 aaaaacatat gattcgtatt gatatgagtg aatatatgga aaaacatgca gtatcaagat
     7681 tgataggggc acctccagga tatattggac atgatgaagg gggtcaatta actgaagcgg
     7741 ttcgtcgtaa tccatactca gttatcttat tagatgaggt tgaaaaagcg catactgacg
     7801 tctttaatgt attattgcaa attttagatg aaggccgttt aactgattct aaaggacgta
     7861 gcgttgattt taaaaatact attattatta tgacaagtaa tattggatct caagttttat
     7921 tagaaaacgt aaaagagact ggtgaaatta cagaatcaac agaaaaagct gttatgacaa
     7981 atttaaatgc atatttcaaa ccagaaattt tgaatcgtat ggatgacatc gtattattta
     8041 aacctctatc tattgatgac atgagtatga ttgtagacaa aatattaacg caattaaata
     8101 taagattatt agaacagcga atctcaattg aagtttctga tgatgctaaa gcttggctag
     8161 gtcaagaagc ttatgaacct caatacggtg caagaccatt aaaacgtttt gtacaacgcc
     8221 aaattgaaac accattagca cgtatgatga ttaaagaggg attcccagaa ggtacaacga
     8281 ttaaagttaa tttaaattca gacaataact taacgtttaa tgttgaaaaa attcatgaat
     8341 aaaattgaac caagaatgtg atgattaatc gcattctt
//
LOCUS       DAHHSC010000104         8317 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.105, whole genome shotgun sequence.
ACCESSION   DAHHSC010000104 DAHHSC010000000
VERSION     DAHHSC010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 8317)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8317)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8317
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.105"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            95..3049
                     /gene="fdhF"
                     /locus_tag="NBI57_001727"
     CDS             95..3049
                     /gene="fdhF"
                     /locus_tag="NBI57_001727"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HCD1822665.1"
                     /translation="MQEHLVVTLDGKDYLVEPGTNLLEFIKSQDTFVPSICYNESMGP
                     IQTCDTCTVEIDGKIERSCSTVIDRPMTVNTVNNDVKDAQKEALDRILEKHMLYCTVC
                     DYNNGDCEIHNTMDAWGLQHQTYEYKEKPYEKDYGPFYRYDPNQCILCGRCVEACQDI
                     EVNETIRIDWDREHPRVIWDNDVPINESSCVSCGQCATVCPCNAMMEVNMEGNAGYMT
                     DTEPGSLAAMIDLTKKAEPGYGPLFAISDSEAEMRKERIKKTKTVCTYCGVGCSFEVW
                     TKDREILKVQPSHDSPANKIATCVKGKFSWGHINSDQRLTKPLVRKNGEFHEVEWDEA
                     LNVIADNFTSIKEKYGPDALSFISSSKATNEESYLMQKLARQVIGTNNVDNCSRYCQA
                     PATKGLFRTVGHGGDSGSIEDLEKAAMSVLIGTNTAEAHPVIASRMKRAQKLFGQKIH
                     VFDIRKHEMAERADRFYQPKPGTDLAWLSAVTKYIIDHDLHDKAFIDEWVDDFDEYYK
                     SLETFTMAFAEEATGIPESELIKFAEECAKAESVVICWAMGITQQDIGSDSSTAISNL
                     LLVTGNYRRPGTGAYPLRGHNNVQGCSDMGSMPDKITGYQSIEADDIRAKFEKEYGVK
                     LNPKAGKDNHEMVEGIHDGEVHSLYLYGEDTGIVDSNINFVQAAFEKLDFMVVQDEFL
                     TFTATYADVVLPASPSLEKDGTFTNTERRIQRLYQALEPLGDSKPDWKIFQAIANRLG
                     FDWNYKHPSEIMDEVARLTPLYAGVSYDRLEGFNSLQWPVQPDGTDEPILYLEGFNFD
                     NGKAKLFPLSFDNYFKQDEIYDIHVNNGRLLEHFHEGNMTYQTPMIKYKVPRAFVEIS
                     PELAEDRGIHEGAEVKLISETGEAVLQVHVTDRVKGKEIYIPLNNDAMENGDLGAINL
                     LTNSDVDQYTDTPSYKRTSCRLEVITKRGKSPLNPNNFRVNKKRHPQYSVQVQKKWER
                     SDYVFPGNQVDK"
     gene            3049..3522
                     /locus_tag="NBI57_001728"
     CDS             3049..3522
                     /locus_tag="NBI57_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1641 domain-containing protein"
                     /protein_id="HCD1822666.1"
                     /translation="MAERISSKIRRLEKSEEQIKLESLNEVTEAIAANKDSILKAIKL
                     IKTLDDAKLLDALNGAIRGRQVIINKFAVELNKDIYTGLLSNMASMVFLLGELNVSDL
                     SDFLNKVNKGLHVANQASPNAKTTIRSLLGVLKDDDMNRSLTYMLNMLKGMSREE"
     gene            3750..3983
                     /locus_tag="NBI57_001729"
     CDS             3750..3983
                     /locus_tag="NBI57_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1822667.1"
                     /translation="MLKTSAVISNTLLVIGLVCLFMMKLVLAITFFAVSLSISLVVFN
                     MMFRDRTTMKVIVNASFLIVILAIVVAYFLLSK"
     gene            4005..4781
                     /locus_tag="NBI57_001730"
     CDS             4005..4781
                     /locus_tag="NBI57_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000739218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosaminidase"
                     /protein_id="HCD1822668.1"
                     /translation="MKKNFKLRISTLLLIVILVVFAVLLIVNETKLFKNDVNYSFDEA
                     VSMQQGKGIVQTKEEDGKFVEANNNEIAKAMTISHKDNDMKYMDITEKVPMSESEVNQ
                     LLKGKGILENRGKVFLEAQEKYEVNVIYLVSHALVETGNGKSELAKGIKDGKKRYYNF
                     FGIGAFDSSAVRSGKSYAEKEQWTSPDKAIIGGAKFIRNEYFENNQLNLYQMRWNPEN
                     PAQHQYASDIRWADKIAKLMDKSYKQFGIKKDDIRQTYYK"
     gene            5092..6216
                     /locus_tag="NBI57_001731"
     CDS             5092..6216
                     /locus_tag="NBI57_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HCD1822669.1"
                     /translation="MKIAIIGAGIGGLTAAALLQEQGHTIKVFEKNESVKEIGAGIGI
                     GDNVLKKLGNHDLAKGIKNAGQILSTMTVLDDKDRLLTTVKLKSNTLNVTLPRQTLID
                     IIKSYVKDDVIFTNHEVTHIDNETDKVTIHFAEQESEAFDLCIGADGIHSKVRQSVNA
                     DSKVLYQGYTCFRGLIDDIDLKHPDCAKEYWGRKGRVGIVPLLNNQAYWFITINSKEN
                     NHKYSSFGKPHLQAYFNHYPNEVREILDKQSETGILLHNIYDLKPLKSFVYGRTILLG
                     DAAHATTPNMGQGAGQAMEDAIVLVNCFNAYDFEKALQRYDKIRVKHTAKVIKRSRKI
                     GKIAQYRSRLFVAVRNRIMKMMPNALAAGQTKFLYKSKEK"
     gene            complement(6308..7261)
                     /locus_tag="NBI57_001732"
     CDS             complement(6308..7261)
                     /locus_tag="NBI57_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase family protein"
                     /protein_id="HCD1822670.1"
                     /translation="MEKVYVAGAIPEVGLKLLQEHFEVEMYEGKGLVDKDTLIKGVKN
                     ATALISLLSTNVDKDVIDAGKDLKIIANYGAGFNNIDIEYAREKSIDVTNTPKASTNA
                     TADLTIGLVLAVARRIVEGDQLSRTTGFDGWAPLFFRGREVSGKTIGIIGLGEIGSAV
                     ARRARAFDMDVLYTGPNRKEEKEREIGAKYVDLDTLLKNADFITINAAYNPKMHHLID
                     TEQFKMMKSTVYLINASRGPIVHEQALVQALKDNEIEGAALDVYEFEPDITDDLKSLN
                     NVVLTPHIGNATFEARDMMSKIVANAAISAVQGEKPQFVVN"
     gene            complement(7622..8122)
                     /locus_tag="NBI57_001733"
     CDS             complement(7622..8122)
                     /locus_tag="NBI57_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HCD1822671.1"
                     /translation="MKKLVTATTLTAGIGTALVGQAHHADAAENYTNYNNYNYNTTQT
                     TTTTTTTTTTSSISHSGNLYTAGQCTWYVYDKVGGEIGSTWGNANNWAAAAQGAGFTV
                     NHTPSKGAILQSSEGPFGHVAYVESVNSDGSVTISEMNYSGGPFSVSSRTISASEAGN
                     YNYIHI"
BASE COUNT         2903 a         1189 c         1603 g         2622 t
ORIGIN      
        1 tctgaaagat gtgtggggca tcgttatttt aggtggatat gagcaattta ttaaaagtca
       61 tttacggaaa atatatatag acggggtgag taatatgcaa gaacatttgg tggttacact
      121 tgatggaaaa gattatcttg tagaaccagg tacgaattta cttgaattta ttaaatcaca
      181 agatactttt gtaccttcaa tttgttataa cgagtcgatg ggaccaattc aaacatgtga
      241 tacatgtact gttgagattg acggtaaaat tgaacgttca tgtagtacgg tgattgatcg
      301 tccaatgact gtaaatactg tgaacaatga tgtgaaagat gctcaaaaag aagcgcttga
      361 tcgaatttta gaaaagcata tgctgtattg tacagtatgt gattataata atggtgattg
      421 tgaaattcat aatacgatgg atgcatgggg gcttcagcat caaacgtatg aatataaaga
      481 gaagccatat gaaaaggatt atggcccatt ttatcgttat gatccaaatc aatgtatctt
      541 atgtgggcgt tgtgtagaag catgtcaaga tatcgaagtg aatgaaacta ttagaattga
      601 ttgggatcgt gaacatccac gtgttatttg ggataatgat gtaccaatta atgaatcgtc
      661 atgtgtatct tgtggtcaat gcgcaactgt atgtccatgt aatgccatga tggaagtgaa
      721 tatggaaggt aatgcgggtt atatgactga tacggaacct ggttcattag cagcaatgat
      781 tgatttaact aaaaaagcag aacctggtta tggtccacta tttgcaatct cagattcaga
      841 agcagaaatg cgtaaagagc gtattaagaa aactaaaaca gtgtgtactt attgtggtgt
      901 gggttgttca tttgaagttt ggactaaaga tagagaaatt ttaaaagtac aaccatcaca
      961 tgattctcca gcaaataaaa ttgcgacttg tgttaaaggt aagttttcat ggggacatat
     1021 taattcagat caacgcttaa ctaaaccact agtaaggaaa aatggtgagt tccatgaagt
     1081 agaatgggat gaagctttaa atgtcattgc agataatttt acatctatta aagaaaagta
     1141 tggcccagat gcactatcat tcatttcttc ttctaaagcg acgaatgaag aatcgtattt
     1201 aatgcaaaaa ttagcaagac aagttattgg cacaaataac gttgataact gttcaagata
     1261 ttgccaagca cctgcaacaa aaggcttatt tagaacggtt ggacacggcg gtgactcagg
     1321 tagtattgaa gatttagaaa aagcggcaat gtctgtattg ataggtacca atacagccga
     1381 agctcatcca gttatcgcat cacgcatgaa acgtgctcaa aaattatttg gacaaaaaat
     1441 acatgtattt gatattagaa aacatgaaat ggcagaacgt gcggatcgtt tttatcaacc
     1501 taaaccaggt acggatttag cgtggttaag tgcagtgact aagtatatta ttgatcatga
     1561 tttacacgat aaagcattta ttgatgagtg ggtagatgat tttgatgaat attacaaatc
     1621 attagaaaca tttacaatgg cttttgctga agaagcaaca ggtattcctg aatcagaatt
     1681 gattaaattt gctgaagaat gtgctaaagc tgaatctgtt gtaatttgtt gggcaatggg
     1741 tattacacaa caagacattg gtagtgactc aagtacagcg atttcaaact tattattagt
     1801 aacaggtaat tatcgtcgtc ctggtactgg tgcgtatcca ttacgtggac ataataatgt
     1861 tcaaggatgt agtgatatgg gaagtatgcc tgataagatt actggttatc aaagtattga
     1921 agcggatgat attcgcgcta aattcgaaaa agaatatggc gttaaattga atccaaaagc
     1981 tggtaaagat aatcatgaaa tggtagaagg tatacatgac ggagaagtac actcattgta
     2041 cttatatggt gaagatacgg gtattgtgga ttcaaatatt aattttgtac aagctgcgtt
     2101 tgaaaaatta gatttcatgg tagtccaaga tgaattttta acattcacag caacatacgc
     2161 agatgttgta ttgccagcaa gtccttcact tgaaaaagac ggtactttta caaataccga
     2221 acgtcgtatt caacgtttat accaagcatt agaacctctt ggtgattcaa aacctgactg
     2281 gaaaattttc caagcaattg ctaatagatt aggatttgat tggaattaca agcatcctag
     2341 tgaaattatg gatgaagtag cacgcttaac acctctatat gctggggtaa gttatgatcg
     2401 tttagaagga ttcaatagtt tacaatggcc agtacaacct gatggtactg atgagcctat
     2461 tctatactta gaaggattca attttgataa tggtaaagca aaattattcc cattatcatt
     2521 tgataattac tttaagcaag atgaaattta tgatattcat gtaaacaatg gtcgattgtt
     2581 agagcacttc catgaaggta atatgactta tcaaacacca atgattaaat acaaagtgcc
     2641 acgtgcattt gttgaaattt ctccagagct tgctgaagat agaggcattc atgaaggtgc
     2701 agaagttaag ctgatttctg aaacaggaga agcggtatta caagttcacg tgacagatcg
     2761 tgttaaaggt aaagaaatct atattccatt aaataatgat gcgatggaaa atggagatct
     2821 aggtgcaatt aacttattaa ctaatagtga tgttgatcaa tacacggata caccatctta
     2881 taaacgtact agctgtcgtt tggaggtaat tacgaaacgt ggtaagtcac cgttgaaccc
     2941 taataatttc cgtgtcaata aaaaacgtca tccgcagtac agtgttcaag tacagaaaaa
     3001 atgggaacgt tcagattatg ttttcccggg aaatcaggtg gataaataat ggctgagaga
     3061 atatcttcta aaattagacg cttagaaaaa tcagaagaac aaattaagtt agaaagttta
     3121 aatgaagtta cagaggcaat tgcagcgaat aaagatagta tattaaaagc aatcaagctt
     3181 attaaaacat tagatgatgc aaaactatta gatgctttaa atggtgcaat tcgaggacgt
     3241 caagtcatca taaataaatt tgcagttgaa cttaataaag atatttatac agggctatta
     3301 tctaatatgg cttcaatggt atttttatta ggtgaactca atgtatcaga tttaagcgac
     3361 ttcctaaata aagttaataa aggattacac gttgccaatc aagcgagtcc aaatgctaag
     3421 acaactataa gaagtctatt gggcgtattg aaagatgacg acatgaaccg tagtttaaca
     3481 tacatgctta atatgctaaa aggtatgtct cgagaagaat aacattacaa tgaaaaagtg
     3541 tttctacatg caagcagtgt aggagcactt tttttaattt ttgtaaaaat taaaaagggt
     3601 gtttttgatg gaataaatga ataatattga aaaacaaaaa aatcacgtgc tattgtgaat
     3661 caatcatcga caggcctatg aaacatcatg tattattatg attatgtaag ttatttaaat
     3721 aaaaagggtg agtgcatatt aattcgagaa tgttaaaaac gtcagctgta atttctaata
     3781 cattattagt cataggttta gtctgtctat ttatgatgaa attagtgtta gctattactt
     3841 tttttgctgt ttcattatca attagtctag ttgtctttaa tatgatgttc agagatagaa
     3901 caacgatgaa agtaatcgtt aacgcttctt ttttaatcgt aatattagcg attgtagtgg
     3961 catatttttt attgtctaag taagaaaaat gaggttttgt ctatatgaag aagaatttca
     4021 agttacgcat ttcaacgcta ctattgatag ttattttagt tgtttttgct gtattactca
     4081 tcgtgaatga aactaaattg tttaaaaatg atgtgaatta ctcttttgat gaggctgttt
     4141 caatgcaaca agggaaaggt attgtacaga caaaagaaga ggatggtaaa tttgttgaag
     4201 caaataataa tgaaattgct aaagcaatga ctatttcaca taaagacaat gatatgaagt
     4261 atatggatat cacagaaaaa gtgccaatgt cggaatctga agttaaccaa ttgctaaaag
     4321 gtaaggggat tttagaaaat cgagggaaag tttttctaga agctcaagaa aaatatgagg
     4381 ttaatgtcat ttatcttgtt agccatgcat tagtagaaac aggtaacggc aaatcagaat
     4441 tagcaaaagg cattaaagat gggaaaaaac gctattacaa cttttttggt ataggagcat
     4501 tcgatagtag tgctgttcgt agtgggaaaa gttatgctga aaaggaacaa tggacatcac
     4561 cagataaggc gattattggt ggtgcaaagt tcattcgtaa tgaatatttt gaaaacaatc
     4621 aactgaattt atatcaaatg cgatggaatc cagaaaatcc tgcgcaacat caatatgcga
     4681 gtgacattcg atgggcagat aaaattgcca aattaatgga taaatcctat aagcagtttg
     4741 gtataaagaa agatgatatt agacaaacat attataaata agacatcggt gcttaaagga
     4801 gctggaacaa tttattgttt cgagctcctt tagcgcattc tgagtgtgtt agttaaatgg
     4861 attttaacct aacaaaaaac gctatatagc atcaaatatg ctatatccca catcattgtt
     4921 acaaatgtac atgatgtaaa tgaatattgc tgtctaaatg tgcatgtaat gtacaatggt
     4981 gcagataata cacttaagtc cttaaaaatg aaacgttagt tccaagagtc atttttaaac
     5041 aatagtgcat gtgataaaat agaaaagaat gaaaaatata gaggtgacaa tatgaagata
     5101 gcaattatag gtgcaggcat cggtggatta acagctgctg cattattaca agaacaaggt
     5161 catactatta aagtctttga aaaaaatgag tcagttaaag aaattggcgc tgggattggt
     5221 atcggagata atgtgcttaa aaaactaggt aatcatgact tagctaaagg tattaaaaat
     5281 gctgggcaaa tcttatctac aatgacagtg ttagatgaca aagatcgcct gttaactact
     5341 gttaaattaa aaagtaatac attgaatgtg acgttaccac gccaaacatt aattgacatt
     5401 attaaatctt atgtaaaaga tgatgtaata tttacaaatc atgaagttac gcatatagat
     5461 aatgagacag ataaagttac catacatttc gcggaacaag agagtgaagc atttgattta
     5521 tgtattggtg ctgatggaat tcattctaaa gtgagacaat ctgtaaatgc tgacagtaaa
     5581 gtattatatc aagggtatac atgctttaga ggtttaattg atgatattga tttaaagcat
     5641 ccggattgtg caaaagaata ctggggaaga aaaggaagag taggtattgt tccgttatta
     5701 aataatcaag catattggtt cattacaatt aactcgaagg aaaacaatca taaatatagt
     5761 tcgtttggta aacctcattt gcaagcatac tttaatcact atccaaatga agttagagag
     5821 atcttggaca aacaaagtga aacaggtatc ttattgcata atatttatga tttgaaacca
     5881 ctcaaatctt ttgtttatgg tcgtactatt ttactaggag atgcagcaca tgcgacaacg
     5941 cctaatatgg ggcaaggtgc tggacaagca atggaagatg ctatcgtatt agtaaattgt
     6001 tttaacgcat acgactttga aaaagcatta cagcgttatg ataaaatacg tgtcaaacat
     6061 actgcaaaag taattaagcg ttctagaaaa atcggtaaaa ttgcccaata tcgtagtcgt
     6121 ttatttgttg cagttagaaa tcgtattatg aaaatgatgc caaatgcatt agcagctgga
     6181 caaactaaat tcttatataa atcgaaagag aaataataca acaatatgaa aacccccgta
     6241 tgttgaaacg agagctcaac atatgggggt tcttgttttt ataatgttat tataataaat
     6301 tcaattatta gttaacgaca aattgtggtt tctcaccttg aacggcacta attgcagcat
     6361 tagcaacaat tttagacatc atgtcacgtg cttcaaatgt agcattacca atatgcggtg
     6421 ttaatactac attattaagt gattttaagt catcggtaat atctggttca aattcatata
     6481 catcaagtgc agcaccttca atttcattat ctttcaatgc ttgcactagt gcttgttcgt
     6541 gcacgattgg accacgagag gcattgatta aatacaccgt agatttcatc attttaaatt
     6601 gttctgtatc aattaaatga tgcattttag gattataagc agcgttgata gtgataaaat
     6661 ctgcattctt taatagtgta tctaaatcta catattttgc accgatttct cgttcttttt
     6721 cttctttgcg attaggtcca gtgtatagca catccatgtc aaatgctctt gcacgacgag
     6781 ctactgcact accaatttca cctaaaccga taatgccgat tgttttccca gatacttctc
     6841 tacctctgaa aaataaaggt gcccatccgt caaatccagt tgtacgtgat aattggtccc
     6901 cttcaacaat acgacgagct actgcaagta ctaatccaat tgttaaatca gcagtcgcgt
     6961 ttgttgatgc tttaggtgtg tttgtaacat ctatactttt ttctcgggca tactcgatat
     7021 caatattatt aaaaccagcg ccatagttgg caatgatttt taagtcttta ccagcatcga
     7081 taacatcttt atcaacgttt gtagataata aactaattaa ggcagtcgcg tttttaacac
     7141 ctttaattaa agtgtcttta tcgactaatc ctttaccttc atacatttca acttcaaaat
     7201 gttcttgtaa aagttttaac cctacttctg gtattgcacc agcaacataa actttttcca
     7261 taaaagatca ctccttttat cttagtatag tagaagatta gacagtatac aactatgtca
     7321 tgatgtcttg tgtatcaatg atgtaagcgc gtacttttga tggaggtgat ataacttagg
     7381 cactgtagaa ctatgaattt tgtaatgtgg aaaaactgga tcaattaaat tagataacgt
     7441 agttttaaag ttaatagtat tagaaaaaat taatattata aatatggggg aaatataaat
     7501 aagtaggtgg caacgaaaaa tagcaaaaaa agagcttctc ctataaagga aagctcaaag
     7561 ttttttgatg acatatgtac tagaattaag tttcaagaca atatgtatca tcgtgtttat
     7621 attaaatatg gatgtagttg tagttacctg cttcacttgc agaaatagtt ctagaactta
     7681 ctgagaaagg tccgccacta taattcattt ctgaaattgt aactgaacca tcactgttta
     7741 cactttctac atatgcaacg tgaccaaatg gtccttcaga agattgtagg atagcgcctt
     7801 tagaaggtgt atgatttact gtgaatccag caccttgtgc agcagcagcc caattattag
     7861 catttcccca agtagaaccg atttctccgc ctactttatc atatacatac caagtacatt
     7921 gtcctgcagt gtataagtta ccagaatgtg aaattgatga tgtagttgtc gtagttgttg
     7981 tagtcgttgt agtttgagtc gtgttgtagt tatagttgtt gtaatttgta taattttcag
     8041 cagcatctgc atgatgtgct tgacctacta atgctgtacc gattcctgct gttaacgtag
     8101 ttgctgttac taattttttc atgaataaag tcctccaaag ttctatatct ttttttataa
     8161 ataaaacgta gcgactgttt tattctcaca tctcgaattg atgacaatag ttactttaac
     8221 aaaattaatg cttcttgtgg ggaatgttat tgatttgtaa aagaataaaa aaactttgac
     8281 taattttgta ataaaaatta gtcaaagtta caatgag
//
LOCUS       DAHHSC010000105         8299 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.106, whole genome shotgun sequence.
ACCESSION   DAHHSC010000105 DAHHSC010000000
VERSION     DAHHSC010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 8299)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8299)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8299
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.106"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            126..959
                     /locus_tag="NBI57_001734"
     CDS             126..959
                     /locus_tag="NBI57_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HCD1822672.1"
                     /translation="MNKTVKDLILVVLGSFIFAAGVNAFIISGNLGEGGVTGLAIILY
                     YAFHISPAITNFLVNAVLIAIGYKFLSKRSMYLTILVTILISIFLSLTESWQVETGNS
                     IVNAIFGGVSVGLGIGVIILAGGTTAGTTILARIATKYLDVSTPYALLFFDMIVVAIS
                     LTVIPLDKVLVTVISLYIGTKVMEYVIEGLNTKKAMTIISTNPDKLAKAIDEQIGRGL
                     TILNGHGYYTREEKDVLYVVISKTQVSKAKRLIKQIDKDAFLVIHDVRDVYGNGFLAD
                     E"
     gene            1241..2038
                     /locus_tag="NBI57_001735"
     CDS             1241..2038
                     /locus_tag="NBI57_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCD1822673.1"
                     /translation="MNRLHGQQVKIGYGDNTIINKLDVEIPDGKVTSIIGPNGCGKST
                     LLKALSRLLAVKEGEVFLDGENIHTQSTKEIAKKIAILPQSPEVADGLTVGELVSYGR
                     FPHQKGFGRLTAEDKKEIDWAMEVTGTDTFRHRSINDLSGGQRQRVWIAMALAQRTDI
                     IFLDEPTTYLDICHQLEILELVQKLNQEQGCTIVMVLHDINQAIRFSDHLIAMKEGDI
                     IATGSTEDVLTQEILEKVFNIDVVLSKDPKTGKPLLVTYDLCRRAYS"
     gene            2074..3078
                     /locus_tag="NBI57_001736"
     CDS             2074..3078
                     /locus_tag="NBI57_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HCD1822674.1"
                     /translation="MTNRENPTPLKFLSYIIGLSMILLITLFISTLIGDAKIQASTII
                     EAIFNYNPSNQQQNIINEIRIPRNIAAVIVGMALAVSGAIIQGVTRNGLADPALIGLN
                     SGASFALALTYAVLPNTSFLILMFAGFLGAILGGAIVLMIGRSRRDGFNPMRIILAGA
                     AVSAMLTALSQGIALAFRLNQTVTFWTAGGVSGTTWSHLKWAIPLIGIALFIILTISK
                     QLTILNLGESLAKGLGQNVTMIRGICLIIAMILAGIAVAIAGQVAFVGLMVPHIARFL
                     IGTDYAKILPLTALLGGILVLVADVIARYLGEAPVGAIISFIGVPYFLYLVKKGGRSI
                     "
     gene            3075..4091
                     /locus_tag="NBI57_001737"
     CDS             3075..4091
                     /locus_tag="NBI57_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HCD1822675.1"
                     /translation="MISSNNKRRQLIALAVFSILLFLGCTWSITSGEYNIPVERFFKT
                     LIGQGDAIDELILLDFRLPRMMITILAGAALSISGAIVQSVTKNPIAEPGILGINAGG
                     GFAIALFIAIGKINADNFVYVLPLISILGGITTALIIFIFSFNKNEGVTPASMVLIGV
                     GLQTALYGGSITIMSKFDDKQSDFIAAWFAGNIWGDEWPFVIAFLPWVLIIIPYLLFK
                     SNTLNIIHTGDNIARGLGVRLSRERLILFFIAVMLSSAAVAVAGSISFIGLMGPHIAK
                     RIVGPRHQLFLPIAILVGACLLVIADTIGKIVLQPGGVPAGIVVAIIGAPYFLYLMYK
                     TKNV"
     gene            4325..5293
                     /gene="dhaK"
                     /locus_tag="NBI57_001738"
     CDS             4325..5293
                     /gene="dhaK"
                     /locus_tag="NBI57_001738"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HCD1822676.1"
                     /translation="MMKKLINKKETFLTDMLEGLLIAHPELDLIANTVIVKKAKKEHG
                     VAIVSGGGSGHEPAHAGFVAEGMLDAAVCGEVFTSPTPDKILEAIKAVDTGDGVLLVV
                     KNYAGDVMNFEMAQELAEMEGINVQTVIVRDDIAVTNEVQRRGVAGTVFVHKLAGYLA
                     EKGYSLTEIKSRVEALLPEIKSIGMAIEPPLVPTTGKYGFDIEDDKMEIGIGIHGEKG
                     IHREEVKDIDHIVGTLLDELYKEVTANDVILMVNGMGGTPLSELNIVTKYIQQNLAAR
                     TVNVAKWFVGDYMTSLDMQGFSITIVPNKPEYLEAFLAPTTSQYFK"
     gene            5335..5919
                     /gene="dhaL"
                     /locus_tag="NBI57_001739"
     CDS             5335..5919
                     /gene="dhaL"
                     /locus_tag="NBI57_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit L"
                     /protein_id="HCD1822677.1"
                     /translation="MKVNDMKARLLNLEETFKKHESELTELDRAIGDGDHGVNMVRGF
                     SSLKDKLDDSSMQSLFKSTGMALMSNVGGASGPLYGFSFVKMSAVTKDDMDNQDFITL
                     IQAFAEAVESRGKVTLNEKTMYDVVARAAEKLENGETLTFNDLQQLADNTKDMVATKG
                     RAAYFGEESKGYIDPGAQSMVYILNALIGDEDNA"
     gene            5912..6274
                     /locus_tag="NBI57_001740"
     CDS             5912..6274
                     /locus_tag="NBI57_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase
                     phosphotransferase subunit DhaM"
                     /protein_id="HCD1822678.1"
                     /translation="MPKIILVSHSKEIASGTKPLLKQMAGDVDIIPIGGLPDGSIGTS
                     FDIIQEVLTKLEDDALCFYDIGSSEMNVDMAIEMYDGNHRVLKVDAPIVEGSFIAAVK
                     LSIGGSIDDALAEIKQSF"
     gene            6390..6887
                     /locus_tag="NBI57_001741"
     CDS             6390..6887
                     /locus_tag="NBI57_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1822679.1"
                     /translation="MQHLIKKHVLNGEFDLVRQLMSETDFMEFEEAYISSAHEVESMM
                     FYTCILDMIKYEESSEMHDLAFLLLVYPLSEYEGALDSAYYHADASIKLTDGKEVKSL
                     LQMLLLHAIPTPVISDKKAFDIAKQILKLDPNNNVARNVLKDTAKRMDNVVVDINELH
                     QRNAR"
     gene            7344..>8299
                     /locus_tag="NBI57_001742"
     CDS             7344..>8299
                     /locus_tag="NBI57_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1822680.1"
                     /translation="MMMNKEATKIGFAYVGIVVGAGFSTGQEVMQFFTKYGLWAYLGV
                     IISGFILAFIGRQVAKIGTAFEATNHESTLQYVFGEKFSKVFDYILIFFLFGIAVTMI
                     AGAGATFEESYNIPTWLGALIMTLAIYITLLLDFNKIVRALGIVTPFLIVLVVLIAGV
                     YLFKGHVSLAEVNQVVPEASIWKGIWFGTIYGGLAFSVGFSTIVAIGGDTEKRTVSGA
                     GAMYGGIIYTVLLALINFALQSEYPTIKNASIPTLTLANNIHPLIATVLSVIMLAVMY
                     NTILGLMYSFAARFTEPYSKNYHIFIIIMMVAGYLLSFVGFA"
BASE COUNT         2758 a         1156 c         1632 g         2753 t
ORIGIN      
        1 gctattcttc atataaatta gttaaatagt aaatctttgt tagaactctt tccatagagt
       61 tgtgacagca tttttgttgt gctactattt ttatagtcta ataaatatat aaaggggatg
      121 gtttcgtgaa taaaacggtt aaagatttaa tactagttgt cttaggttca tttatctttg
      181 ctgcaggtgt aaatgcattt attatttctg gtaacttagg tgaaggcggg gttacaggtt
      241 tagcaattat tttatattat gcgtttcata tttcaccagc catcactaac ttcttggtca
      301 acgcagtatt gattgccata ggttataaat ttttgagtaa gagaagtatg tacttaacta
      361 ttcttgtaac aattcttatt tcaatatttt tgagtttaac agaatcatgg caagtagaaa
      421 ctggaaacag cattgtgaat gccatttttg gtggtgtaag cgttggacta ggaatcggag
      481 taattatcct tgcaggcggt acaacagcag gtacaacaat tttggcgaga attgcaacga
      541 aatacctcga tgtaagcacg ccatatgctt tgcttttctt cgatatgatc gttgttgcaa
      601 tttcacttac agttattcca cttgataaag tattagtaac agtaatatca ctttatatag
      661 gaacaaaagt gatggaatat gtcatagaag gtttaaacac taaaaaagct atgacgatta
      721 tttcaactaa tcccgacaaa cttgccaaag caatagacga gcaaattgga agaggtttaa
      781 ccattttaaa cggacatggc tattatacgc gtgaagaaaa agatgtctta tacgttgtta
      841 tttctaaaac acaagtttca aaagcaaagc gattaattaa acaaatcgat aaagatgcat
      901 tcctcgtaat tcatgatgta agagatgtct atggtaatgg ctttcttgca gatgaataaa
      961 taaatggtat gagcacacat acttaaatag aagtccacgg acaagttttt gaactatgaa
     1021 gacttatctg tgggcgtttt ttattttata aaagtaatat acaagacatg acaaatcgag
     1081 ctatccaatt taaaaagtaa tgttagtcaa taagattgaa aaatgttata atgatgttca
     1141 tgataatcat tatcaattgg gatgcctttg aaaattgata atttaaaaat agaaattatt
     1201 ttttataaac agaaagaatt ttattgaaag tagggaaatt atgaatcgtt tgcatggaca
     1261 acaagttaaa attggttacg gggataacac gattataaat aaattagatg ttgaaatacc
     1321 agatggcaaa gtgacgtcaa tcattggtcc taacggctgc gggaaatcta ctttgctaaa
     1381 ggcattgtca cgtttattgg cagttaaaga aggcgaagta tttttagatg gtgaaaatat
     1441 tcatacacaa tctacgaaag agattgcaaa aaaaatagcc attttacctc aatcacctga
     1501 agtagcagat ggcttaactg ttggggaatt agtttcatat ggtcgttttc cacatcaaaa
     1561 aggatttggt agattaactg ctgaggataa gaaagaaatt gattgggcaa tggaagttac
     1621 aggaactgat acattccgac accgttcaat caatgattta agtggtggtc aaagacaacg
     1681 tgtttggatt gcaatggcat tagcacaaag aactgatatt atctttttag acgaaccaac
     1741 aacatattta gatatctgtc atcaattaga aatactagaa ttagttcaga agctaaatca
     1801 ggaacaaggt tgtacaattg tcatggttct tcatgatatc aaccaagcga ttcgtttctc
     1861 agatcatctt attgcgatga aagaagggga tatcatcgct acaggttcaa cagaagacgt
     1921 attaacacag gaaatattag aaaaagtttt taatattgat gttgttttaa gtaaagatcc
     1981 taaaactgga aaacctttac tggtaactta tgacttatgt cgcagagctt attcttaatt
     2041 aagtaagtta atatgataaa aaggacaatt aacatgacaa atagagagaa cccaacgcca
     2101 ttgaagtttt tatcctatat tataggttta agtatgatac tactaatcac actatttatt
     2161 tctacattaa taggtgacgc caaaattcaa gcctctacaa ttatagaggc tatttttaat
     2221 tataatccta gcaatcaaca gcaaaacatc atcaatgaga ttaggattcc cagaaatata
     2281 gcagcagtaa ttgtaggtat ggcgcttgca gtttctggtg cgattataca aggtgttact
     2341 cgtaatggtc ttgctgatcc ggcgctcata ggtttaaatt caggtgcttc atttgcttta
     2401 gcattaacat atgcagtttt accaaacact tcatttttaa tattgatgtt tgctggattt
     2461 ttaggtgcta ttctaggagg tgctattgta ttaatgatag gccgatctag acgtgatgga
     2521 tttaatccga tgcgtattat tttagcgggt gcagcagtaa gtgctatgtt aacagcgcta
     2581 agtcaaggta ttgcattagc ttttagacta aatcaaacag taacattttg gactgctgga
     2641 ggcgtttcag gcacaacatg gtcacacctt aagtgggcaa ttccattaat tggtattgcg
     2701 ttattcatta tattaacaat tagtaaacaa cttaccattt taaatcttgg tgaatcatta
     2761 gctaaaggtt taggtcaaaa tgtaacaatg atcagaggca tatgtttaat tattgctatg
     2821 attctagcag gtattgcagt tgctatcgct ggacaagttg catttgtagg tttgatggta
     2881 cctcatatag caagattttt aattggaact gattatgcta aaattctacc attaacagcc
     2941 ttgttaggtg ggatactcgt gcttgttgcc gatgtgatag cacgatattt aggagaagcg
     3001 cctgttggtg caatcatttc atttatcggt gttccttact ttttatattt agttaaaaaa
     3061 ggaggacgct caatatgatt agttcaaata ataaacgcag acaattgata gcactggctg
     3121 tttttagcat tctactattt ctaggttgta cttggagtat tacctcaggt gaatacaaca
     3181 tacctgttga aagatttttc aaaactttaa ttggacaagg tgatgccatt gatgagttaa
     3241 tcttattaga tttcaggtta cctcggatga tgattactat tttggctggc gcagcgctta
     3301 gtattagtgg tgcaatagtg caaagtgtca caaaaaatcc aatagctgaa ccaggtatat
     3361 taggtattaa cgcaggtggc ggatttgcaa tcgcattatt tattgcaatt ggtaaaatta
     3421 atgctgacaa ctttgtttat gtactgccgt taataagtat actaggtggt atcaccactg
     3481 cattgattat ttttattttc agttttaata aaaatgaagg tgttacacct gcgagtatgg
     3541 tattaatagg tgtaggttta caaacagcat tatatggtgg ctcaattaca attatgtcaa
     3601 aatttgatga taagcaatct gatttcatcg ctgcttggtt tgcaggtaat atttggggtg
     3661 acgaatggcc atttgtcatt gcatttttac cgtgggtgtt gattattatt ccttacttac
     3721 tatttaaatc gaatacacta aatattattc atacgggtga taatattgca cgaggtctag
     3781 gtgtaaggtt aagcagagaa cgtttaatat tattctttat cgcagtgatg ttatcatctg
     3841 ctgctgtagc agtagcaggt tcaatttcgt ttatcggatt aatgggtccg catattgcca
     3901 aacgtatcgt tggaccacgt caccagttgt ttttaccaat tgccatttta gtaggggcat
     3961 gtttacttgt tatagctgat acaattggca aaattgtatt acaaccaggt ggggttccag
     4021 caggtattgt cgtagcaatt attggtgcac cgtatttctt atatttaatg tacaaaacga
     4081 aaaatgtata gtgtcaaagg acacaactta ttgctatgaa aggcacttta ttataaggct
     4141 tttcatagca ttttttattt aatgagccac tcaagactat ttattttttc aataatgaac
     4201 cattaagtta tcaagaggat cttatcaaaa atatatttga taacggtatc aggttaattc
     4261 tttatgatag cgcattcatt tattctgttt tatactatga ctgataatac caaggaggta
     4321 caacatgatg aaaaagttaa tcaataaaaa agaaacattt ttaactgata tgcttgaagg
     4381 attgttaatt gcgcacccag agttagatct gattgctaat acagttattg taaaaaaagc
     4441 taagaaagaa catggtgtag caatagtctc tggaggtgga agcggacatg aacctgcgca
     4501 tgccggtttt gttgcagaag gtatgctaga tgcagcggtt tgtggcgaag tatttacatc
     4561 acctacacct gataaaatat tagaagctat taaagcagta gatactggtg atggtgtatt
     4621 actagttgta aaaaactatg caggtgacgt gatgaatttc gaaatggcac aagagcttgc
     4681 agaaatggaa ggtataaatg ttcaaactgt tattgttcgt gacgacattg ctgtgacaaa
     4741 cgaagtacaa cgtcgtggtg ttgcaggaac agtgtttgtt cataagcttg ccggttatct
     4801 tgctgaaaaa ggttattcat taacagagat aaaatcgcgt gtagaagcgt tgttacctga
     4861 aattaaaagt attggtatgg caattgagcc accgcttgtt ccaactactg gaaaatatgg
     4921 ctttgatatt gaagacgaca aaatggaaat cggtattggt atacatggtg aaaaaggtat
     4981 tcatagggaa gaagtaaagg atattgatca tattgttgga acattgttag acgaattgta
     5041 taaagaagtt actgccaatg atgtcatatt aatggtaaat ggtatgggtg gtacgccgtt
     5101 atctgaatta aatatcgtaa ctaaatatat tcaacaaaat ttagctgcaa gaacggttaa
     5161 tgttgctaaa tggtttgttg gtgattatat gacatcttta gacatgcaag gtttttctat
     5221 aactatcgtg cctaataaac cagaatattt ggaagcattt ttagcaccaa caacaagtca
     5281 atactttaaa taagaagtga atatgaataa atatacattt atgaggtggc acaaatgaaa
     5341 gtgaatgata tgaaagcacg tttattaaat ttagaagaaa cgtttaaaaa acatgaatct
     5401 gaattaactg aattagatcg agcaattggt gatggtgacc acggggttaa catggttcgt
     5461 gggtttagta gtcttaaaga caaacttgat gatagctcaa tgcaatcatt gttcaaatcg
     5521 actggtatgg cattgatgtc aaatgttggg ggtgcatcag gaccactgta tggctttagc
     5581 tttgttaaaa tgtctgcagt caccaaagat gatatggata atcaagattt cattacacta
     5641 attcaggcat ttgccgaagc ggttgaatca cgtggtaaag ttactttaaa tgaaaagaca
     5701 atgtatgatg tagtagcgcg agcagcagag aagcttgaaa atggtgaaac tttaacattc
     5761 aatgatttac agcaattagc agataataca aaagatatgg tagcaacgaa aggtagagct
     5821 gcatattttg gagaagaatc aaaaggttat attgatccag gtgctcaaag tatggtttat
     5881 attttaaacg ctttgattgg agatgaagat aatgcctaaa attatacttg ttagccacag
     5941 taaagaaatt gcaagtggta caaaaccttt gttaaagcaa atggcaggtg acgttgatat
     6001 tataccaatc gggggattac cagatggttc aattggaact tcatttgata tcatccaaga
     6061 agttttgact aaattagagg atgatgcatt gtgtttttac gatattggat cttcagaaat
     6121 gaatgtagat atggcaattg aaatgtatga tggtaatcat cgtgtgttaa aagttgatgc
     6181 accaattgtt gaaggcagtt ttatcgcagc agtaaagcta tcaatcggcg gttcaattga
     6241 tgatgcatta gcagaaatca aacaatcatt ttagttaaaa tttactaata atgaaaaatg
     6301 taaacctttt tcaaatgaaa ctttataaaa aatatgatag tatatatgta aatgtttaat
     6361 aaaatctgga gaaataggag gacattgcca tgcaacacct tataaaaaaa catgtattga
     6421 atggcgagtt tgatttagta cgacaattga tgtccgaaac agattttatg gaatttgaag
     6481 aagcatatat ttcaagtgcg catgaagtag aaagtatgat gttttataca tgtattttag
     6541 atatgattaa gtacgaagaa tcatctgaaa tgcatgactt agcattttta ttgcttgtgt
     6601 atccactaag tgaatatgaa ggtgctttgg attctgctta ttatcatgca gacgcttcca
     6661 taaaacttac tgacggcaaa gaagttaaaa gtttgttaca aatgttatta ttgcatgcga
     6721 taccaacacc tgttatttca gataagaagg cttttgatat cgccaagcaa attttaaaat
     6781 tagatcctaa taataatgtt gctcgtaacg tcttaaaaga cactgccaaa cgtatggaca
     6841 acgttgttgt tgatataaat gaattacacc aacgtaatgc acgttaatta catttcaatt
     6901 atattagctt aataatagtt ttaacatttg gttgggttgg gacatatgtt ccagcctttt
     6961 ttaatactta aaaactaacg aagtatactt gtgtgcacaa atggttttta tacaacattt
     7021 tataaattta tacattttaa taaagaacat acgatatatg gtttaaacct tgttaactga
     7081 gagattttga tatgtattct tcgaaattta actaaattta cgaaattcaa gaagcacaat
     7141 aattaatcaa ttttcctata caaaagttcg tatgactgca ttataaaagc ataaatttat
     7201 aattttttta aatgtcattg aacgtgataa tgtgaatgga ttgagcaatt ttgaaaaagt
     7261 gaaaaataac ctatgcgact tgcaattaat tttcagtacg ttataatgca cactgtgcaa
     7321 aattaaggag gtctattatt cacatgatga tgaataaaga agcaacaaaa attggatttg
     7381 cctacgtcgg cattgtagtg ggcgcaggat tttcaactgg acaagaagtt atgcaatttt
     7441 tcactaaata tggcttgtgg gcttatttag gtgttattat atctggtttt attttagctt
     7501 ttattgggcg ccaagtagca aaaattggta ctgcctttga agcgacaaac catgaatcaa
     7561 cattacaata cgtattcggt gaaaagttta gtaaagtctt tgattatatt ttaatcttct
     7621 tcttatttgg tatagctgta accatgatag ctggtgcagg cgcaacattt gaagaaagtt
     7681 ataacatacc tacatggcta ggtgctttaa ttatgacatt agcgatttat attacgttgc
     7741 tattagactt taataaaata gtacgtgcac ttggtatcgt tacaccattt ttaattgttt
     7801 tagttgtatt aatcgctggc gtttatttat ttaaaggtca tgtttcatta gcagaagtta
     7861 accaagtagt gcctgaagca agtatttgga agggaatctg gtttggtaca atatatggtg
     7921 gattagcttt ttctgtaggt tttagtacca tcgtagcaat cggtggggat acagaaaagc
     7981 gtacagtgtc aggtgcaggc gcgatgtatg gtggtattat ctatactgta ttactagcat
     8041 tgatcaactt tgcattgcaa agtgaatatc caactattaa aaatgcctca attccaacat
     8101 tgacgttagc aaataatatc catcctttaa tagcaacagt gttatctgtt attatgctgg
     8161 cggttatgta taatactatt ctaggactaa tgtattcatt tgcagcacgt tttacagaac
     8221 catacagtaa aaattatcat atctttatta ttataatgat ggtagcaggt tatttattaa
     8281 gtttcgtagg atttgctga
//
LOCUS       DAHHSC010000106         8273 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.107, whole genome shotgun sequence.
ACCESSION   DAHHSC010000106 DAHHSC010000000
VERSION     DAHHSC010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 8273)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8273)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8273
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.107"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            97..723
                     /locus_tag="NBI57_001743"
     CDS             97..723
                     /locus_tag="NBI57_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkyA family protein"
                     /protein_id="HCD1822681.1"
                     /translation="MKFGKTIAVVLASSVLLAGCTTDKKEIKAYLKQVDKIKDDEEPI
                     KTVGKKIAELDEKKKKLTEDVNSKDTAVRGKAVKDLIKNADDRLKEFEKEEDAIKKSE
                     QDFKKAKSHVDNIDNDVKRKEVKQLDDVLKEKYKLHGEYAKAYKKAVNSEKTLFKYLN
                     QNDATQQGVNEKSKAIEQNYKKLKEVSDKYTKVLNKVGKEKQDVDQFK"
     gene            894..2006
                     /gene="pdhA"
                     /locus_tag="NBI57_001744"
     CDS             894..2006
                     /gene="pdhA"
                     /locus_tag="NBI57_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002467471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HCD1822682.1"
                     /translation="MAPKLQAQFDAVKVLNDTQSKFEMVQILDENGNVVNEDLVPDLT
                     DEQLVELMERMVWTRILDQRSISLNRQGRLGFYAPTAGQEASQLASQYALEKEDYILP
                     GYRDVPQIIWHGLPLTEAFLFSRGHFKGNQFPEGVNALSPQIIIGAQYIQAAGVAFAL
                     KKRGKNAVAITYTGDGGSSQGDFYEGINFAAAYKAPAIFVIQNNNYAISTPRSKQTAA
                     ETLAQKAIAVGIPGIQVDGMDALAVYQATKEARDRAVAGEGPTLIETMTYRYGPHTMA
                     GDDPTRYRTSDEDAEWEKKDPLVRFRKFLENKGLWNEDKENEVIERAKADIKAAIKEA
                     DNTEKQTVTSLMEIMYEDMPQNLAEQYEIYKEKESK"
     gene            2010..2987
                     /locus_tag="NBI57_001745"
     CDS             2010..2987
                     /locus_tag="NBI57_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017637695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HCD1822683.1"
                     /translation="MAQMTMVQAINDALKTELKNDQDVLIFGEDVGVNGGVFRVTEGL
                     QKEFGEDRVFDTPLAESGIGGLAMGLAVEGFRPVMEVQFLGFVFEVFDAIAGQIARTR
                     FRSGGTKTAPVTIRSPFGGGVHTPELHADNLEGILAQSPGLKVVIPSGPYDAKGLLIS
                     SIRSNDPVVYLEHMKLYRSFREEVPEEEYTIDIGKANVKKEGNDISIITYGAMVQESM
                     KAAEELEKDGYSVEVIDLRTVQPIDVDTIVASVEKTGRAVVVQEAQRQAGVGAAVVAE
                     LSERAILSLEAPIGRVAAADTIYPFTQAENVWLPNKNDIIEKAKETLEF"
     gene            3078..4370
                     /locus_tag="NBI57_001746"
     CDS             3078..4370
                     /locus_tag="NBI57_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HCD1822684.1"
                     /translation="MAFEFRLPDIGEGIHEGEIVKWFVKAGDTIEEDDVLAEVQNDKS
                     VVEIPSPVSGTVEEVMVEEGTVAVVGDVIVKIDAPDAEDMQFKGHDDDSSSKEEPAKE
                     EAPAEQAPVATQTEEVDENRTVKAMPSVRKYAREKGVNIKAVSGSGKNGRITKEDVDA
                     YLNGGAPTASNESAASATSEEVAETPAAPAAVSLEGDFPETTEKIPAMRRAIAKAMVN
                     SKHTAPHVTLMDEIDVQALWDHRKKFKEIAAEQGTKLTFLPYVVKALVSALKKYPALN
                     TSFNEEAGEIVHKHYWNIGIAADTDRGLLVPVVKHADRKSIFQISDEINELAVKARDG
                     KLTADEMKGATCTISNIGSAGGQWFTPVINHPEVAILGIGRIAQKPIVKDGEIVAAPV
                     LALSLSFDHRQIDGATGQNAMNHIKRLLNNPELLLMEG"
     gene            4374..5780
                     /gene="lpdA"
                     /locus_tag="NBI57_001747"
     CDS             4374..5780
                     /gene="lpdA"
                     /locus_tag="NBI57_001747"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009383646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HCD1822685.1"
                     /translation="MVVGDFPIETDTIVIGAGPGGYVAAIRAAQLGQKVTIVEKGNLG
                     GVCLNVGCIPSKALLHASHRFVEAQHSENLGVIAESVSLNFQKVQEFKSSVVNKLTGG
                     VEGLLKGNKVNIVKGEAYFVDNNSLRVMDEKSAQTYNFKNAIIATGSRPIEIPNFKFG
                     KRVIDSTGALNLQEVPGKLVVVGGGYIGSELGTAFANFGSEVTILEGAKDILGGFEKQ
                     MTQPVKKGMKEKGVEIVTEAMAKSAEETDNGVKVTYEAKGEEKTIEADYVLVTVGRRP
                     NTDELGLEELGVKFADRGLLEVDKQSRTSISNIYAIGDIVPGLPLAHKASYEAKVAAE
                     AIDGQAAEVDYIGMPAVCFTEPELATVGYSEAQAKEEGLAIKASKFPYAANGRALSLD
                     DTNGFVKLITLKEDDTLIGAQVVGTGASDIISELGLAIEAGMNAEDIALTIHAHPTLG
                     EMTMEAAEKAIGYPIHTM"
     gene            5948..6223
                     /locus_tag="NBI57_001748"
     CDS             5948..6223
                     /locus_tag="NBI57_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HCD1822686.1"
                     /translation="MEYEYPIDLDWSNEEMISVINFFNHVEKYYESGVTAGDFMGAYK
                     RFKEIVPAKAEEKQIFNTFEKSSGYNSYKAVQDVKTHSEEQRVTAKK"
     gene            6367..6906
                     /locus_tag="NBI57_001749"
     CDS             6367..6906
                     /locus_tag="NBI57_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCD1822687.1"
                     /translation="MNIGNKIKNLRRIKNLTQEELAERTDLSKGYISQIESEHASPSM
                     ETFLNIIEVLGTTPSEFFKDSENEKVLYKKEEQVIYDEYDEGYILNWLVSKSNEYDME
                     PLILTLKPGASYKNFNPSESDTFIYCMSGQITLNLGKEIYQAQEEDVLYFKARDNHRL
                     SNESNNETRILIVATASYL"
     gene            6919..8013
                     /locus_tag="NBI57_001750"
     CDS             6919..8013
                     /locus_tag="NBI57_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCD1822688.1"
                     /translation="MEPLLSLKSVSKSYDDLNILDDIDIDIESGYFYTLLGPSGCGKT
                     TILKLIAGFEYPDSGEVIYQNKPIGNLPPNKRKVNTVFQDYALFPHLNVYDNIAFGLK
                     LKKLSKTEIDQKVTEALKLVKLSGYEKRNINEMSGGQKQRVAIARAIVNEPEILLLDE
                     SLSALDLKLRTEMQYELRELQSRLGITFIFVTHDQEEALALSDFLFVLKDGKIQQFGT
                     PTDIYDEPVNRFVADFIGESNIVEGRMVRDYVVNIYGQDFECVDMGIPENKKVEVVIR
                     PEDISLIKAEEGLFKATVDSMLFRGVHYEICCIDNKGYEWVIQTTKKAEVGSEVGLYF
                     DPEAIHIMVPGETEEEFDKRIESYEEVDNA"
     gene            8006..>8273
                     /locus_tag="NBI57_001751"
     CDS             8006..>8273
                     /locus_tag="NBI57_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCD1822689.1"
                     /translation="MRNTNKFLLIPYLLWMVIFIIVPVVLLIYFSFLDINGHFSFTNY
                     QQIFTTKYLKMFAYSILYAALITIITLAISYPAAYYITRSKFQNI"
BASE COUNT         3029 a         1196 c         1631 g         2417 t
ORIGIN      
        1 cttagtaatg aagatgtgta atgtaattgt ttaaaattga tttccaagca gattttattt
       61 atcatttaat ttaaatagca agtggaggta caagtaatga aatttggaaa aacaatcgca
      121 gtagtattag catctagtgt cttgcttgca ggatgtacta cggataaaaa agaaattaag
      181 gcatatttaa agcaagtgga taaaattaaa gacgatgaag aaccaattaa aactgttggt
      241 aagaaaattg ctgaattaga tgagaaaaag aaaaaattaa ctgaagatgt caatagtaaa
      301 gatacagcag ttcgcggtaa agcagtaaaa gatttaatta aaaatgccga tgatcgtcta
      361 aaggaatttg aaaaagaaga agacgcaatt aagaagtctg aacaagactt taagaaagca
      421 aaaagtcacg ttgataacat tgataatgat gttaaacgta aagaagtaaa acaattagat
      481 gatgtattaa aagaaaaata caaattgcat ggtgaatacg cgaaagcata taaaaaggct
      541 gtaaactcag agaaaacatt atttaaatat ttaaatcaaa atgacgcgac acaacaaggt
      601 gttaacgaaa aatcaaaagc aatagaacag aactataaaa agttaaaaga agtatcagat
      661 aagtatacga aagtactaaa taaggttggt aaagaaaagc aagacgttga tcaatttaaa
      721 taattaatat aatacagatg gtaggaaaca actaatacag ttcctattat ctgtatcttt
      781 ttttattaaa acagaacttt ttcaaatggt ttaacagtcc catttatttg tggtacaatt
      841 agtaaggata aaatgaattt ctatacaatt atgggaaagg tatggtgaat tgaatggctc
      901 ctaagttaca agcccaattc gatgcagtaa aagttttaaa tgatactcaa tcgaaatttg
      961 aaatggttca aattttggat gagaatggta acgtcgtaaa tgaagactta gtacctgatc
     1021 ttacggatga acaattagta gaattaatgg aaagaatggt atggactcgt atccttgatc
     1081 aacgttctat ctcattaaac agacaaggac gtttaggttt ctatgcacca actgctggtc
     1141 aagaagcatc acaattagcg tcacaatacg ctttagaaaa agaagattac attttaccgg
     1201 gatacagaga tgttcctcaa attatttggc atggtttacc attaactgaa gctttcttat
     1261 tctcaagagg tcacttcaaa ggaaatcaat tccctgaagg cgttaatgca ttgagcccac
     1321 aaattattat cggtgcacaa tacattcaag ctgctggtgt tgcatttgca cttaaaaaac
     1381 gtggtaaaaa tgcagttgca atcacttaca ctggtgacgg tggttcttca caaggtgatt
     1441 tctacgaagg tattaacttt gcagcagctt ataaagcacc tgcaattttc gttattcaaa
     1501 acaataacta tgcaatttca acaccaagaa gcaagcaaac tgctgctgaa acattagctc
     1561 aaaaagcaat tgctgtaggt attcctggta tccaagttga tggtatggat gcgttagctg
     1621 tatatcaagc aactaaagaa gcacgtgacc gcgcagttgc aggtgaaggt ccaacattaa
     1681 ttgaaactat gacatatcgt tatggtcctc atacaatggc tggtgacgat ccaactcgtt
     1741 acagaacttc agacgaagat gctgaatggg agaaaaaaga cccattagta cgtttccgta
     1801 aattccttga aaacaaaggt ttatggaatg aagacaaaga aaatgaagtt attgaacgtg
     1861 caaaagctga tattaaagca gcaattaaag aggctgataa cactgaaaaa caaactgtta
     1921 cttctctaat ggaaattatg tatgaagata tgcctcaaaa cttagcagaa caatatgaaa
     1981 tttacaaaga gaaggagtcg aagtaagcca tggcacaaat gacaatggtt caagcgatta
     2041 atgatgcgct taaaactgaa cttaaaaatg accaagatgt tttaattttt ggtgaagacg
     2101 ttggtgttaa cggcggtgtt ttccgtgtta ctgaaggact acaaaaagaa tttggtgaag
     2161 atagagtatt cgatacacct ttagctgaat caggtattgg tggtttagcg atgggtcttg
     2221 cagttgaagg attccgtccg gttatggaag tacaattctt aggtttcgta ttcgaagtat
     2281 ttgatgcgat tgctggacaa attgcacgta ctcgtttccg ttcaggcggt actaaaactg
     2341 cacctgtaac aattcgtagc ccatttggtg gtggcgtaca cacaccagaa ttacacgcag
     2401 ataacttaga aggtatttta gctcaatctc caggtctaaa ggttgttatt ccttcaggcc
     2461 catacgatgc gaaaggttta ttaatttctt ctattagaag taatgaccca gtcgtatact
     2521 tagagcatat gaaattgtat cgttcattcc gtgaagaagt acctgaagaa gaatatacaa
     2581 ttgacattgg taaggctaat gtgaaaaaag aaggtaatga catttcaatc atcacatacg
     2641 gtgcaatggt tcaagaatca atgaaagctg cagaagaact tgaaaaagat ggttattctg
     2701 ttgaagtaat tgacttacgt actgttcaac caatcgatgt tgacacaatt gtagcttcag
     2761 ttgaaaaaac tggtcgtgca gttgtagttc aagaagcaca acgtcaagct ggtgttggtg
     2821 cagcagttgt agctgaatta agtgaacgtg caatcctttc attagaagca cctattggaa
     2881 gagttgcagc agcagataca atttatccat tcactcaagc tgaaaatgtt tggttaccaa
     2941 acaaaaatga catcatcgaa aaagcaaaag aaactttaga attttaatac attttaaaag
     3001 ttaacgaagt tagcgtattt tagtctcatt gattaaaatg aaatgtttaa tttacgaaat
     3061 cttaggaggg caaaaacgtg gcatttgaat ttagattacc cgatatcggg gaaggtatcc
     3121 acgaaggtga aattgtaaaa tggtttgtta aagctggaga tactattgaa gaagacgatg
     3181 ttttagctga ggtacaaaac gataaatcag tagtagaaat cccatcacca gtatctggta
     3241 ctgtagaaga agttatggta gaagaaggta cagtagctgt agttggtgac gttattgtta
     3301 aaatcgatgc acctgatgca gaagatatgc aatttaaagg tcatgatgat gattcatcat
     3361 ctaaagaaga acctgcgaaa gaggaagcgc cagcagagca agcacctgta gctactcaaa
     3421 ctgaagaagt agatgaaaac agaactgtta aagcaatgcc ttcagtacgt aaatacgcac
     3481 gtgaaaaagg tgttaacatt aaagcagttt ctggatctgg taaaaatggt cgtattacaa
     3541 aagaagatgt agatgcatac ttaaatggtg gtgcaccaac agcttcaaat gaatcagctg
     3601 cttcagctac aagtgaagaa gttgctgaaa ctcctgcagc acctgcagca gtatcattag
     3661 aaggcgactt cccagaaaca actgaaaaaa tccctgctat gcgtagagca attgcgaaag
     3721 caatggttaa ctctaagcat actgcacctc atgtaacatt aatggatgaa attgatgttc
     3781 aagcattatg ggatcaccgt aagaaattta aagaaatcgc agctgaacaa ggtactaagt
     3841 taacattctt accttatgtt gttaaagcac ttgtttctgc attgaaaaaa tacccagcac
     3901 ttaacacttc attcaatgaa gaagctggtg aaattgtgca taaacattat tggaatatcg
     3961 gtattgcggc ggacactgat aggggtttat tagtacctgt agtgaaacat gctgatcgta
     4021 agtctatttt ccaaatttca gatgaaatta atgaattagc tgttaaagca cgtgatggta
     4081 aattaacagc cgatgaaatg aaaggtgcaa catgcacaat cagtaatatc ggttcagctg
     4141 gtggacaatg gttcactcca gttatcaatc acccagaagt agcaatctta ggaattggcc
     4201 gtattgctca aaaacctatc gttaaagatg gagaaattgt tgcagcacca gtattagcat
     4261 tatcattaag ctttgaccac agacaaattg atggtgcaac tggccaaaat gcaatgaatc
     4321 acattaaacg tttattaaat aatccagaat tattattaat ggaggggtaa aacatggtag
     4381 ttggagattt cccaattgaa acagatacta tagtaatcgg agcaggtcct ggtggatacg
     4441 ttgcagcaat tcgtgcagct caattaggac aaaaagtaac aatcgttgag aaaggtaatc
     4501 ttggtggtgt ttgcttaaac gtaggatgta ttccttcaaa agcattacta catgcttctc
     4561 accgttttgt tgaagcacaa cattctgaaa acttaggtgt tattgctgaa agtgtttctt
     4621 taaacttcca aaaagttcaa gaattcaaat catcagttgt taataaatta actggtggtg
     4681 ttgaaggctt acttaaaggt aacaaagtta acatcgttaa aggtgaagca tatttcgtag
     4741 ataacaatag cttacgtgtt atggacgaaa agagcgcaca aacatacaac tttaaaaatg
     4801 caatcattgc aacaggttca agaccaattg aaattcctaa tttcaaattc ggtaaacgtg
     4861 ttatcgactc aacaggtgct ttaaacttac aagaagtacc aggtaaatta gttgtagttg
     4921 gtggaggata cattggatca gaattaggta cagcatttgc taactttggt tcagaagtaa
     4981 ccatccttga aggtgctaaa gatatcttag gtggcttcga aaaacaaatg acacaacctg
     5041 ttaaaaaagg tatgaaagaa aaaggtgttg aaatcgttac tgaagctatg gctaaatcag
     5101 ctgaagaaac agataacgga gttaaagtta cttatgaagc taaaggcgaa gagaaaacaa
     5161 tcgaagctga ttatgtatta gtaactgtag gtcgtcgtcc aaacacagac gaattaggcc
     5221 tagaagaatt aggtgttaaa ttcgctgacc gtggattatt agaagttgat aaacaaagcc
     5281 gtacgtctat cagcaatatc tatgcaattg gtgatatcgt tccaggttta ccacttgctc
     5341 acaaagctag ctatgaagct aaagttgctg ctgaagcaat tgatggtcaa gctgctgaag
     5401 ttgattacat tggtatgcca gcagtatgct ttactgaacc agaattagct acagttggtt
     5461 attcagaagc gcaagctaaa gaagaaggtt tagcaattaa agcttctaaa ttcccatatg
     5521 cagcaaatgg tcgtgcatta tcattagatg atactaacgg atttgttaaa cttattacac
     5581 ttaaagaaga tgatacttta atcggtgctc aagtagttgg tactggtgca tcagatatta
     5641 tctctgaatt aggtttagca attgaagctg gtatgaatgc tgaagatatc gcattaacaa
     5701 tccatgcaca tccaacatta ggtgagatga ctatggaagc agcagaaaaa gctatcggat
     5761 acccaatcca tacaatgtaa taactgatta tctataaaga ttcagtcatt aaaagctgta
     5821 gcatatgcta cggctttttt gttttaggta aagtaatgta aggaaattga tttgagatat
     5881 cgttaacatg tgacatgcat gttatactag cgatgctaac aaaagaattg aaatggaggg
     5941 ttcaacaatg gaatatgagt atccaattga tttagactgg agtaatgaag agatgatttc
     6001 agtgataaat ttctttaatc atgtagagaa gtattatgaa tccggcgtga cggcaggcga
     6061 ctttatgggt gcatataaaa gatttaaaga aattgtgcct gctaaagcag aggaaaaaca
     6121 aatttttaat actttcgaaa aaagtagtgg ctataatagt tacaaagcag ttcaagatgt
     6181 aaaaactcac tctgaagaac aaagagtaac agctaaaaaa taattcgttc gaaattaaca
     6241 caatttaata ggattttttc tttaaaacta ttgctaataa agctatattt tgataccttt
     6301 atcaagtgtt aaacaaaatg tttgataaaa gtaaacttaa tatagctttt ttaggtggaa
     6361 aaataaatga acataggtaa taaaattaaa aatcttagaa gaattaaaaa tttaacgcaa
     6421 gaagaacttg ctgaacgtac agacttatcg aaaggctaca tttcacaaat agaaagtgaa
     6481 catgcctcac caagtatgga aactttctta aatattatag aggtgttagg aacgacgcca
     6541 agtgaatttt ttaaagacag tgaaaatgaa aaagtattat acaagaagga agaacaagtt
     6601 atttatgatg agtatgatga aggttatata ttaaattggt tagtttcaaa gtcaaatgaa
     6661 tatgatatgg agccattaat attaacttta aagcctggag catcatataa aaattttaat
     6721 ccatcagagt ctgatacgtt tatttattgt atgtcaggtc agataacact taatttaggc
     6781 aaagagatat atcaagcaca agaagaagac gttttgtatt ttaaagcacg agataatcat
     6841 cgtttgtcaa acgaatcaaa caatgaaaca cgaatactta ttgtagcgac agcttcatat
     6901 ttataggggg gatcttattt ggaaccgtta ttatcattaa aatcagttag taaaagctat
     6961 gatgatctta atatcttaga tgacatagat attgatattg aatcaggata cttttataca
     7021 ttattaggtc cttcaggttg tggtaaaaca acaattttaa aattaattgc agggtttgaa
     7081 tatcctgaca gtggtgaagt gatttatcaa aacaaaccaa ttggtaattt accaccaaat
     7141 aaacgtaaag tgaatacagt ctttcaagat tatgcattat ttccacactt aaacgtatat
     7201 gataatatcg cttttggttt gaaattaaaa aaattatcaa aaaccgaaat tgatcaaaaa
     7261 gtaactgagg cattaaaatt agtaaaactt tcaggttatg aaaaaagaaa tattaatgaa
     7321 atgagtggcg gacaaaagca acgtgttgca attgcacgtg ctatcgtaaa tgaaccagaa
     7381 atattattgt tagatgaatc tttatccgca ttagatttga aattgcgtac tgaaatgcaa
     7441 tatgaattac gagaattgca atctagatta ggtattacat ttatatttgt aacacatgat
     7501 caagaagaag cgttagcatt aagtgacttt ctttttgtat taaaagatgg gaaaattcaa
     7561 caatttggca caccaacaga tatatatgac gaaccagtga atcgatttgt agctgatttt
     7621 attggagaat ctaatattgt tgaaggacgc atggttagag attatgtcgt gaatatttat
     7681 gggcaagatt tcgaatgtgt cgatatgggt attcctgaaa ataaaaaagt agaagtcgtt
     7741 attcgaccag aagatatatc attaatcaaa gctgaagaag gattatttaa agcaactgtt
     7801 gattctatgt tatttagagg ggtccactat gaaatatgtt gtatagacaa taaaggttat
     7861 gaatgggtaa tacaaacgac taaaaaagct gaagtaggca gtgaagttgg tctttatttt
     7921 gatcctgaag ccattcatat tatggttcct ggagaaacag aagaagaatt tgataaacgt
     7981 attgaaagct atgaggaagt agacaatgcg taatactaat aaatttctct taatcccgta
     8041 tttactatgg atggttatat ttattattgt accagttgta ttactcattt atttttcatt
     8101 tttagatatc aatggacatt ttagtttcac gaattatcaa caaattttta ctacaaaata
     8161 tttgaaaatg tttgcatatt caattttata tgccgcttta ataacaatta ttacgttggc
     8221 tatcagttat ccagctgcct attatattac tcgttcgaaa tttcaaaata tct
//
LOCUS       DAHHSC010000107         8255 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.108, whole genome shotgun sequence.
ACCESSION   DAHHSC010000107 DAHHSC010000000
VERSION     DAHHSC010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 8255)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8255)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8255
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.108"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            104..715
                     /locus_tag="NBI57_001752"
     CDS             104..715
                     /locus_tag="NBI57_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin"
                     /protein_id="HCD1822690.1"
                     /translation="MFKKYDSKNSIVLKSILSLGIIYGGTFGIYPKADASTQNSSSVQ
                     DKQLQKVEEVPNNSEKALVKKLYDRYSKDTINGKSNKSRNWVYSERPLNENQVRIHLE
                     GTYTVADRVYTPKRNITLNKEVVTLKELDHIIRFAHISYGLYMGEHLPKGNIVINTKD
                     GGKYTLESHKELQKDRENVKINTADIKNVTFKLVKSVNDIEQV"
     gene            1084..1452
                     /locus_tag="NBI57_001753"
     CDS             1084..1452
                     /locus_tag="NBI57_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HCD1822691.1"
                     /translation="MKTILKTITYLALTIIGAYAALFILKTIDSHGITDQFNPLVKED
                     DSYVKTTEVSTRMDDQLRSYTQSAFNKEGKETQLMYTATFDVKPHRYLKITHKGHHVE
                     TFEEVEKEEVPKKALDKLSR"
     gene            1633..2205
                     /locus_tag="NBI57_001754"
     CDS             1633..2205
                     /locus_tag="NBI57_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HCD1822692.1"
                     /translation="MKLKSLAVLSMSAVVLTACGNDTPKDETKSTESNTNQDTNTTKD
                     VIALKDVKTSPEDAVKKAEETYKGQKLKGISFENSNGEWAYKVTQQKSGEESEVLVAD
                     KNKKVINKKTEKEDTVNENDNFKYSDAIDYKKAIKEGQKEFDGDIKEWSLEKDDGKLV
                     YNIDLKKGNKKQEVTVDAKNGKVLKSEQDH"
     gene            complement(2342..2605)
                     /locus_tag="NBI57_001755"
     CDS             complement(2342..2605)
                     /locus_tag="NBI57_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCD1822693.1"
                     /translation="MNMHILYNLRTKHNLEIDELAQQLNEKYGTKYEAHQIWEWENHH
                     HEPKFKDAMHLADFFDAPYEMFLESKVKEYQKHLEEVDIRMDK"
     gene            2905..3156
                     /locus_tag="NBI57_001756"
     CDS             2905..3156
                     /locus_tag="NBI57_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HCD1822694.1"
                     /translation="MFGFIGMLIVGGLIGWAAGAIMGKDIPGGILGNIIAGIIGSWVG
                     GKLFGQWGPELGSIYILPALIGSIILIAIVTLILRAMRK"
     gene            complement(3195..3404)
                     /locus_tag="NBI57_001757"
     CDS             complement(3195..3404)
                     /locus_tag="NBI57_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1822695.1"
                     /translation="MTTQSFIFMNIILLDGATQRHPFFICQILAFLFPKFPHINLLLF
                     YPILSTFSNIIIHIDHFHANKKDDN"
     gene            3582..4163
                     /locus_tag="NBI57_001758"
     CDS             3582..4163
                     /locus_tag="NBI57_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HCD1822696.1"
                     /translation="MTIYLVRHGESKSNYDNKHFRSYFCGQLDVPLTDTGTKSADDLC
                     DYFKEKQIKHVYVSDLLRTQQTFEHIFPYDIASTTTPLLRERSLGVFEGEYKDEISAN
                     PKYEKYFNDPNFKDFRHSFSQKAPEGESYEDVYQRVEHFMNHVVNEDTQKDDIVIVAH
                     QVVIRCLMVYFNKVSREEAVDLKVENCKPYIIE"
     gene            complement(4229..4612)
                     /locus_tag="NBI57_001759"
     CDS             complement(4229..4612)
                     /locus_tag="NBI57_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1822697.1"
                     /translation="MKVRITFIILAILSTIVCLFLAAMHPTGPNTVTFEQPYLFTLNI
                     ILMVLVALPSLVLAIYDYMSFRISSAILQFLGAISWFFLSLILSLTQFTPFTLASFIT
                     SIILFISAIITLAIGGKSVDKNDSH"
     gene            complement(4882..5508)
                     /locus_tag="NBI57_001760"
     CDS             complement(4882..5508)
                     /locus_tag="NBI57_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NDxxF motif lipoprotein"
                     /protein_id="HCD1822698.1"
                     /translation="MKKRLLLSTFLASALILTGCASDQSDNEDHHTSTGIHAPKSAKK
                     LETKDIFNSDKKNSDISDAEMKQAIEKYLSVNSDILDNKYIMQHKLDKQIDSQTKVTE
                     KQAETLSHLSNLAVKNDLHFKKFVTENNIPKEYKKPVELMMNYFKALNSTIANVDEDI
                     EKLSYQPQNKINVVDVPTKYAGDVNKKQQDKIKDFLKSKGIKSDVIDK"
     gene            complement(5581..5799)
                     /locus_tag="NBI57_001761"
                     /pseudo
     CDS             complement(5581..5799)
                     /locus_tag="NBI57_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887771.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(5952..7475)
                     /gene="ahpF"
                     /locus_tag="NBI57_001762"
     CDS             complement(5952..7475)
                     /gene="ahpF"
                     /locus_tag="NBI57_001762"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HCD1822699.1"
                     /translation="MLNADLKQQLKQLLELMEGNVEFVASLGSDEKSKELKELLTEIS
                     DMSPRLSLSEKSLKRTPSFSVNRPGEETGVTFAGIPLGHEFNSLVLAILQVSGRAPKE
                     KQSIIDQIKNLEGSFHFETFISLTCQKCPDVVQALNLMSVINPNITHSMIDGAVFREE
                     SENIMAVPAVFLNGEEFGNGRMTIQDILSKLGSTADASEFENKEPYDVLIVGGGPASG
                     SAAIYTARKGLRTGIVADRIGGQVNDTAGIENFITVKETTGSEFSSNLAAHIDQYDID
                     AMTGIRATDIEKTDEAIKVTLENGAVLESKTVIIATGAGWRKLNIPGEEQLINKGVAF
                     CPHCDGPLFENKDVAVIGGGNSGVEAAIDLAGIVNHVTLFEFASELKADNVLQDRLRS
                     LSNVDIKTNAKTTEVVGEDHVTGIRYEDMSTGEEHLLNLDGIFVQIGLLPNTSWLKDA
                     VELNERGEIVIDRNNNTNVPGIFAAGDVTDQKNKQIIISMGAGANAALNAFDYIIRN"
     gene            complement(7491..8060)
                     /gene="ahpC"
                     /locus_tag="NBI57_001763"
     CDS             complement(7491..8060)
                     /gene="ahpC"
                     /locus_tag="NBI57_001763"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002445177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HCD1822700.1"
                     /translation="MSLINKEILPFTAQAFDPKKDQFKEVTQEDLKGSWSVVCFYPAD
                     FSFVCPTELEDLQNQYEELQKLGVNVFSVSTDTHFVHKAWHDHSDAISKITYTMIGDP
                     SQTITRNFDVLDEATGLAQRGTFIIDPDGVVQASEINADGIGRDASTLAHKIKAAQYV
                     RKNPGEVCPAKWEEGAKTLQPGLDLVGKI"
BASE COUNT         2885 a         1202 c         1415 g         2753 t
ORIGIN      
        1 aaattaaagt taatcaagag ttaagatgaa tttaattcat gaacacgtct attattttta
       61 taattgtagc aaataaagct ttacatcaag gaggtaatta aatatgttca aaaaatatga
      121 ctcaaaaaat tcaatcgtat taaaatctat tctatcgcta ggtatcatct atgggggaac
      181 atttggaata tatccaaaag cagacgcgtc aacacaaaat tcctcaagtg tacaagataa
      241 acaattacaa aaagttgaag aagtaccaaa taattcagaa aaagctttgg ttaaaaaact
      301 ttacgataga tacagcaagg atacaataaa tggaaaatct aataaatcta ggaattgggt
      361 ttattcagag agacctttaa atgaaaacca agttcgtata catttagaag gaacatacac
      421 agttgctgat agagtatata cacctaagag aaatattact cttaataaag aagttgtcac
      481 tttaaaggaa ttggatcata tcataagatt tgctcatatt tcttatggct tatatatggg
      541 agaacatttg cctaaaggta acatcgtcat aaatacaaaa gatggcggta aatatacatt
      601 agagtcgcat aaagagctac aaaaagatag ggaaaatgta aaaattaata cagccgatat
      661 aaaaaatgta actttcaaac ttgtgaaaag tgttaatgac attgaacaag tttgaaatta
      721 agttgaatta gtatatacag tgttttattg ctaatacttt gaaagttagg tatctaaagg
      781 tgcctagctt tctttgttat gattagcact atcatataga aaattcttta atgacgttta
      841 tgccgaaatc tacaaatata atttctcttt tatggttaat caatatgttt ttacattcga
      901 caaccttaaa attaacttct ttcaattcca taataaagtc tctataaaat aacttagttt
      961 aaaaacgatt cgtatctttc aggttcaaat accatcattt tctcctaata cttacacttt
     1021 aattacaatt atgtaagttg ttttcagagg catcctattt tatattttgt cagaggtgat
     1081 cttttgaaaa cgattttaaa aacaataaca tatcttgcac ttactatcat tggcgcttat
     1141 gctgctttat tcattttaaa aacaatagac tctcatggta taacagatca atttaaccca
     1201 ttagtaaagg aagatgattc ttatgttaaa acgacagagg tgtctactag aatggatgat
     1261 caactccgaa gttatactca aagtgctttt aataaagaag ggaaagagac gcaattaatg
     1321 tatactgcta catttgatgt taaaccgcat agatacttga aaattacaca taaaggtcat
     1381 catgtagaaa cttttgaaga agttgaaaag gaagaagtac caaagaaagc attagacaaa
     1441 ctgagtcgat aatagcatgc ttatattaca tggttcattt ataaaaggag tacgaacgaa
     1501 agtaacgcat gacgattaat ttaaaaatat ttgtaataat tatgaataaa attaaaaaca
     1561 aggggtaata caatctatat agcatataag cttttgttat gagtttcaaa aataggaaga
     1621 gagagtgata ttatgaaatt aaaatcatta gcagtgttat caatgtcagc ggtggtgctt
     1681 actgcatgtg gcaatgatac tccaaaagat gaaacaaaat caacagagtc aaatactaat
     1741 caagacacta atacaacaaa agatgttatt gctttaaaag atgttaaaac aagcccagaa
     1801 gatgctgtga aaaaagctga agaaacatac aaaggccaaa agttgaaagg aatttcattt
     1861 gaaaattcta atggtgaatg ggcttataaa gtgacgcaac aaaaatctgg tgaagagtca
     1921 gaagtacttg ttgctgataa aaataaaaaa gtgattaaca aaaagactga aaaagaagat
     1981 acagtgaatg aaaatgataa ctttaaatat agcgatgcta tagattacaa aaaagccatt
     2041 aaagaaggac aaaaagaatt tgatggtgat attaaagaat ggtcacttga aaaagatgat
     2101 ggcaaacttg tttacaatat cgatttgaaa aaaggtaata aaaaacaaga agttactgtt
     2161 gatgctaaga acggtaaagt attaaagagt gagcaagatc actaaaaaga ttcgttgcat
     2221 taacgcttgt aaaatgtctt aattcatatt aattaataaa ataccctcac aatacttatg
     2281 cataagtcct gtttgacgtg aaaggatata tgccaaacgt gttgtgaggg tttaattgca
     2341 tctatttatc catgcgaata tcgacttctt ctaaatgttt ctgatattct ttaaccttac
     2401 tttctaaaaa catttcatat ggtgcatcaa agaagtcagc taaatgcatg gcatctttaa
     2461 atttaggttc atgatgatga ttctcccatt cccaaatttg atgtgcttca tatttagtac
     2521 catatttctc atttaattgc tgtgctaatt cgtcaatttc taaattatgt ttagttcgta
     2581 agttatataa aatatgcata ttcattgttc attaacctcg cttcttgatt taaaattcaa
     2641 agatgctcta ataatatgtg tatattctag tactataaca taaaataatc aagctatgaa
     2701 ttcggcagaa aaaataatgt aaatgtatat gatattgcca gtacagagac tttaaataac
     2761 gatagctaca ttgaataaaa ttgatattca attactactt ttaaaaatat ttggataaaa
     2821 ataatttgaa tttgttttag aattgtaaat aaggggtact acttagaata acgcaaaata
     2881 aatagaaaaa ggagactgaa aattatgttt ggatttattg gaatgttaat tgtcggtggc
     2941 ttaattggat gggctgctgg tgctattatg ggtaaagata tcccaggtgg tattttaggc
     3001 aatattatcg caggtattat tggatcatgg gtaggtggca aactattcgg acaatggggt
     3061 cctgaattag gaagtattta catcttgcca gcattaattg gttcaattat cttaattgca
     3121 atcgtaacgt taattttaag agctatgcgt aaataataaa aaaattcaat tggaaattga
     3181 aataaagatg gcattcagtt gtcatctttt ttatttgcgt gaaaatggtc aatatgtatt
     3241 ataatatttg aaaatgtaga taagatagga taaaaaagta ataaattaat atgaggaaat
     3301 tttggaaata aaaaagcgag tatttgacaa ataaaaaaag gatgacgttg ggttgcgcca
     3361 tccaggagta ttatgttcat gaatataaaa gactgtgtag tcatttaaga actacaccca
     3421 tatagtaaca catcacttga ttaaagacaa tactattttt agagtcatta atttaaaata
     3481 tgaattaatt ctttttttag aatagaatat taagattgat atcgaattag tagtcaaagt
     3541 gttatggtag atatgaaata cataaggtaa ggagtaatat tatgacgatt tatttagtta
     3601 gacatggcga atcaaaatcg aattatgata ataaacattt tcgatcttat ttttgtggac
     3661 aattagatgt gccgttaacg gatactggca caaaaagtgc agacgattta tgtgattatt
     3721 ttaaagagaa acagattaaa catgtatatg tttcagactt attaagaaca cagcaaacgt
     3781 ttgaacatat ttttccatat gacattgcat caacgactac ccctctatta agagaacgtt
     3841 cacttggcgt atttgagggt gaatataaag atgaaatcag tgcgaatccg aaatatgaaa
     3901 aatatttcaa tgatccaaac tttaaagact ttcgtcatag tttttcacaa aaagcgcctg
     3961 aaggagaaag ttatgaagat gtatatcaac gcgtagaaca ttttatgaat catgttgtca
     4021 atgaagatac acaaaaagat gatattgtca ttgttgcaca tcaagttgtc attcgttgtt
     4081 tgatggttta ttttaacaaa gtttcaaggg aagaagctgt ggatttaaag gtagaaaatt
     4141 gcaaaccata tatcattgaa tagaagtata gaggttctga aaagctgtgt tttatagtgg
     4201 ccttcagaac ctttttggca tttggaattt aatgggaatc attcttatca acagacttac
     4261 caccaatagc taatgtgata attgcgctta tgaacaaaat aattgaagtt ataaatgacg
     4321 ctaatgtaaa aggtgtaaat tgtgtgagcg ataatatcaa tgataaaaag aaccaagaga
     4381 tagcccctag aaattgtaaa atagcagaag aaattctaaa actcatataa tcatatatag
     4441 ctaaaacaag tgaaggtaat gcgactaata ccattaaaat gatgttgagg gtgaataaat
     4501 atggctgttc aaaagttact gtgtttggtc ctgttggatg cattgctgct aagaacaagc
     4561 aaacaatcgt cgataaaatt gctaaaataa taaaagtaat tcgaactttc atcatgacca
     4621 tcctttgttt atagagtcaa tataagtatg aaatatgtta gggatatagt caaatgcgtc
     4681 aactaatggg tgattttggc atagatagag aatttaaggc aattaaaaaa gcatcaaaca
     4741 gtaatatgct gcttgatgca aaatggtaag gtattggtta aattgtattg tctcaaagat
     4801 tagtcacttt taaagataaa gaattgtcat gaattaaagc tcatgtaatg atgtgttaca
     4861 tgtcgcaatg atggctttca gttatttatc gataacatca ctcttgatac ctttagattt
     4921 taagaaatct ttaattttat cttgttgctt tttattaaca tcaccggcat attttgttgg
     4981 cacgtcgact acattgattt tattttgcgg ttgatagcta agcttttcaa tatcttcatc
     5041 aacattggcg attgtactat ttaaagcttt gaagtaattc atcattaatt caacgggttt
     5101 cttatattct ttaggaatat tgttttcagt gacaaatttc ttgaaatgca aatcgttttt
     5161 aacagctaag ttagataagt ggctaagtgt ttctgcttgt ttttcagtca cttttgtttg
     5221 actgtcaatt tgtttatcta gtttatgttg cataatatat ttgttatcaa gtatatcgct
     5281 atttacagac aaatactttt ctatagcttg cttcatctct gcatcactaa tatcactatt
     5341 tttcttatct gagttaaaga tatcttttgt ttctaatttt ttagcgcttt taggtgcatg
     5401 gatgccagta cttgtgtgat gatcttcgtt atcagattga tcggacgcgc aacctgtaag
     5461 aattaatgca gatgctaaaa atgtacttag tagtaatctc tttttcataa tgtaatataa
     5521 ctccttagtt tatctttaat tgaaaaaata tgtattcatg tttaatagag taacattgaa
     5581 ttagtttgga atgtcacgat gaccttgcaa tgaccataga cgcaaatgat tacgtgcatg
     5641 agttgctttt tctatcaata atgcgtcgtt ttgaacaatg ttaaggatag cattatctat
     5701 aaataactgc ataattggtt gtatcaattt agatgtaggt atcgtacgta aaagcataat
     5761 gatttcgttc acatactttt ccttctcaat atcattttca tattgatttg tttgcgagag
     5821 gtacatactt taagcattat cgcacatctc gttgtatata ttaagtttat cataacatga
     5881 ttttatgtcg ggataaaaaa ataacagcat cttaacaaat gtaagatact gtcagtgaaa
     5941 ctgtgaaaac tttagtttct gataatatag tcaaaggcat ttaatgctgc atttgcacca
     6001 gcgcccattg aaatgataat ttgtttgttc ttctgatctg tgacatcgcc agcagcaaat
     6061 attccaggta cgttagtatt attgttacga tcaataacaa tttcaccacg ttcgtttaat
     6121 tcaacagcat cttttaacca tgatgtgttt ggaagtaaac caatttgaac aaagatacca
     6181 tctaagttaa gtagatgttc ttcgccggtg ctcatgtctt cgtaacgtat acctgtaaca
     6241 tggtcttctc cgacaacttc agtagttttg gcatttgttt tgatatcaac atttgataaa
     6301 gaacgtaaac gatcttgtaa cacgttgtct gcttttaatt cgctagcgaa ttcgaataat
     6361 gtaacatgat taacgatacc agcaaggtca attgctgctt caaccccaga gttaccgcca
     6421 ccgataactg ctacgtcttt attttcgaat agaggtccgt cacagtgagg gcagaatgca
     6481 acacctttat taatcaattg ctcttcacct ggaatgttta gcttacgcca acctgcacca
     6541 gtagcaataa tgactgtttt actttctaag acagcaccgt tttctaacgt aactttaatt
     6601 gcttcgtcag tcttttcgat atctgtagca cgtatacctg tcattgcatc aatgtcatat
     6661 tgatcaatgt gcgctgctaa gttagaagaa aattcagaac cagttgtttc tttaacagta
     6721 atgaagttct caataccagc agtatcatta acttggccac cgatacgatc agcaactata
     6781 ccagtacgta aacctttacg tgctgtgtaa atcgctgcac taccactagc aggaccacca
     6841 ccaacgatta agacatcata aggttcttta ttttcaaact cagatgcatc tgccgtactg
     6901 cctagtttcg aaagaatatc ttggattgtc atacgaccat tgccaaattc ttcgccgttt
     6961 aaaaagacag cagggactgc catgatgttt tcagattctt cacggaacac tgcaccatca
     7021 atcatagaat gcgtgatgtt agggttgatc acactcatta agttaagtgc ttgaacgaca
     7081 tcaggacatt tttgacacgt taaactaatg aatgtttcaa aatggaatga accttctaag
     7141 tttttaattt ggtcaataat tgactgtttt tctttaggtg cacgaccact aacctgtaaa
     7201 attgctaaaa caagtgagtt aaactcgtga cctaatggaa ttcctgcaaa tgttacacct
     7261 gtttcttcgc caggacggtt gactgagaaa cttggtgtac gttttaaaga cttttcagaa
     7321 agtgaaagtc taggtgacat atcagaaatt tctgttaata attctttgag ttccttagat
     7381 ttttcatctg aaccaaggct ggcaacgaat tcaacgttgc cctccattag ttctaataat
     7441 tgtttaagtt gttgttttaa atcagcatta agcatggttg taatgcctcc ttagatttta
     7501 cctactaaat ctaaaccagg ttgcaatgtt ttagcgcctt cttcccattt agctgggcat
     7561 acttcgccag ggtttttacg aacatattga gctgctttga ttttgtgagc taatgtacta
     7621 gcgtcacggc caattccgtc agcgttaatt tcagatgctt gtacaacacc gtctgggtcg
     7681 ataatgaatg taccacgttg agctaaacca gtagcttcat ctaatacatc aaaattacga
     7741 gtgattgttt gtgatgggtc accaatcata gtgtaagtga ttttgctaat tgcatctgaa
     7801 tggtcatgcc atgctttgtg tacgaagtga gtatcagttg atactgagaa tacatttacg
     7861 cctaattttt gtaattcttc atattggttt tgtaagtctt ctaattcagt tggacaaacg
     7921 aatgagaagt cagcaggata gaagcatact acgctccaag aaccttttaa atcttcttgt
     7981 gtaacttctt taaattgatc tttttttgga tcgaaagctt gcgctgtaaa tggtaagatt
     8041 tctttgttaa ttaatgacat aaatatcttc ctcctaagaa tttaagtatg aattagaact
     8101 atcaattgat tgtgcttaat tataataatt ctaatctctt agttagcatt attacatttt
     8161 gatccagaat agtcaactgg ataactttgt aaagtgaacg attactttta aaataaagaa
     8221 agataatata aagtgctttg ataatggatt ttgta
//
LOCUS       DAHHSC010000108         8184 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.109, whole genome shotgun sequence.
ACCESSION   DAHHSC010000108 DAHHSC010000000
VERSION     DAHHSC010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 8184)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8184)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8184
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.109"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            56..6616
                     /gene="sasC"
                     /locus_tag="NBI57_001764"
     CDS             56..6616
                     /gene="sasC"
                     /locus_tag="NBI57_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored repetitive surface protein SasC"
                     /protein_id="HCD1822701.1"
                     /translation="MNLLKKNKYSIRKYKVGIFSTLIGTVLLLSNPNGAQALTTDNNV
                     QSDTNQATPVNSQDTNVANNRGLANSAQNTPNQSATTNQSTNQALVNHNNGSIANQAT
                     PTSVQSSTPSAQNNNHTDGNTTATETVSNANNKDVVSNNTTLNVPNKTNENGSGGHLT
                     LKEIQEDVRHSSDKPELVAIAEQASNRPKKRSRRAAPADPNATPADPAAAAAGNGGAP
                     VAITAPYTPTTDPNANNAGQNAPNEVLSFDDNGIRPSTNRSVPSVTVVDNLPGFTLIN
                     GGKVGVFSHAMVRTSMFDSGDAKNYQAQGNVIALGRIKGNDTNDHGDFNGIEKSLTVN
                     PNSELIFEFNTMTTKNYQGVTNLIIKNADNDTVIAEKSVAYGPIWRLFKVPENVSHLK
                     IQFVPKNDAITDARGIYQLRDGYKYYDFVDSIGLHSGSHVYVERRTMEPTATNNKEFT
                     VTTSLKNNGNFGASFNTDDFVYKVQLPEGVEYVNNSLTKDFPSSNSGVDMNDFNVTYD
                     AANRVITIKSTGGGSGNSPARLMPDKILDLKYKLRVNNVPTPRTVTFNDTLTYKTYTQ
                     DFINSPAESHTVSTNPYTIDIIMNKDALQAEVDRRIQQADYTFASLDIFNDLKRRAQT
                     ILDENRNNVPLNKRVSQADIDSLTNQMQHTLIRSVDAENAVNKKVDQMEDLVNQNDEL
                     TDEEKQAAIQVIEEHKNEIIGNIGDQTTDDGVTRIKDQGIQTLSGDTATPVVKPNAKK
                     AIRDKATKQREIINATPDATEDEIQDALNQLATDETDAIDNVTNATTNADVEIAKNNG
                     INTIGAVVPQVTHKQAARDAINQATATKRQQINSNREATQEEKNAALNELTQATNHAL
                     EQINQATTNADVDNAKGDGLNAINPIAPVTVVKQAARDAVSHDAQQHIAEINANPDAT
                     QEERQAAIDKVNAAVTAANTNILNANTNADVEQVKTNAIQGIQAITPATKVKTDAKNA
                     IDKSAETQHNTIFNNNDATLEEQQAAQQLLDQAVATAKQNINAADTNQEVAQAKDQGT
                     QNIVVIQPATQVKTDARNAVNDKAREAITNINATPGATREEKQEAINRVNTLKNRALT
                     DIGVTSTTAMVNSIRDDAVNQIGAVQPHVTKKQTATGVLNDLATAKKQKINQNTNATT
                     EEKQVALNQVDQELATAINNINQADTNAEVDQAQQLGTKAINAIQPNIVKKPAALAQI
                     NQHYNAKLAEINATPDATNDEKNAAINTLNQDRQQAIESIKQANTNAEVDQAATVAEN
                     NIDAVQVDVVKKQAARDKITAEVAKRIEAVKQTPNATDEEKQAAVNQINQLKDQAINQ
                     INQNQTNDQVDTTTNQAVNAIDNVEAEVVIKPKAIADIEKAVKEKQQQIDNSLDSTDN
                     EKEVASQALAKEKEKALAAIDQAQTNSQVNQAATNGVSAIKIIQPETKVKPAAREKIN
                     QKANELRAKINQDKEATAEERQVALDKINEFVNQAMTDITNNRTNQQVDDTTSQALDS
                     IALVAPEHIVRAAARDAVKQQYEAKKQEIEQAEHATDEEKQVALNQLANNEKLALQNI
                     NQAVTNNDVKRVETNGIATLKGVQPHIVIKPEAQQAIKATAENQVESIKDTPHATVDE
                     LDEANQLISDTLKQAQQEIENTNQDAAVTDVRNQTIKAIEQIKPKVRRKRAALDSIEE
                     NNKNQLDAIRNTLDTTQDERDVAIDTLNKIVNTIKNDIAQNKTNAEVDRTETDGNDNI
                     KVILPKVQVKPAARQSVGVKAEAQNALIDQSDLSTEEERLAAKHLVEQALNQAIDQIN
                     HADKTAQVNQDSIDAQNIISKIKPATTVKATALQQIQNIATNKINLIKANNEATDEEQ
                     NIAIAQVEKELIKAKQQIASAVTNADVAYLLHDEKNEIREIEPVINRKASAREQLTTL
                     FNDKKQAIEANIQATVEERNSILAQLQNIYDTAIGQIDQDRSNAQVDKTASLNLQTIH
                     DLDVHPIKKPDAEKTINDDLARVTALVQNYRKVSNRNKADALKAITALKLQMDEELKT
                     ARTNADVDAVLKRFNVALSDIEAVITEKENSLLRIDNIAQQTYAKFKAIATPEQLAKV
                     KVLIDQYVADGNRMIDEDATLNDIKQHTQFIVDEILAIKLPAEATKVSPKEIQPAPKV
                     CTPIKKEETHESRKVEKELPNTGSEGMDLPLKEFALITGAALLARRRTKNEKES"
     gene            complement(6733..8001)
                     /locus_tag="NBI57_001765"
     CDS             complement(6733..8001)
                     /locus_tag="NBI57_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HCD1822702.1"
                     /translation="MYQTIIIGGGPSGLMAAVAASEQSSSVLLIEKKKGLGRKLKISG
                     GGRCNVTNRLPYAEIIKNIPGNGKFLYSPFSIFDNESIIDFFESRGVKLKEEDHGRMF
                     PVSNKAQDVVDTLVTTIERQHVTIKEEEAVSRIEVNTDQTFTVHTQNNSYESHSLVIA
                     TGGTSVPQTGSTGDGYKFAQDLGHTITELFPTEVPITSAEPFIKSNRLKGLSLKDVEL
                     SVLKKNGKKRISHQMDMLFTHFGISGPAALRCSQFVYKEQKNQKTQHISMAIDAFPEL
                     NHEQLKQHITSLLSDTPDKIIKNSLHGLIEERYLLFMLEQAGIDENTTSHHLSNQQLN
                     DLVNMFKGFVFKVNGTLPIDKAFVTGGGVSLKEIQPKTMMSKLVPGLFLCGEVLDIHG
                     YTGGYNITSALVTGHVAGLYAGHYSHTSME"
BASE COUNT         3220 a         1376 c         1453 g         2135 t
ORIGIN      
        1 tttagcataa ttctaaacaa cacagcagag aacagacaac caggaggaaa atgaaatgaa
       61 tttgttaaag aaaaataaat atagtattag gaagtataaa gtaggcatat tctctacttt
      121 aatcggaaca gttttattac tttcaaaccc aaatggtgca caagccttaa ctacggataa
      181 taatgtacaa agcgatacta atcaagccac acctgtaaat tcacaagata caaatgttgc
      241 taataataga ggtttagcaa atagtgctca gaatacacct aatcaatctg caacaactaa
      301 tcaatcaacg aatcaagcat tggttaatca taataatggt agtatagcaa atcaagctac
      361 gccaacatca gtgcaatcaa gtacgccttc agcacaaaac aataatcata cagatggcaa
      421 tacaacagca actgagacag tgtcaaacgc taataataag gatgtagtgt cgaataatac
      481 cacattaaat gtacctaata aaacaaatga aaatggttca ggaggacatc taactttaaa
      541 ggaaattcaa gaagatgttc gtcattcttc agataaacca gagctagttg caattgctga
      601 acaagcatct aatagaccga aaaagagaag tagacgtgcg gcaccggcag atcctaatgc
      661 aactccagca gatccagcgg ctgcagcggc aggaaacggt ggtgcaccag tagcaattac
      721 agcgccatac acgccgacaa ctgatcctaa tgccaataat gcaggacaaa atgcacctaa
      781 cgaagtgctg tcttttgatg acaatggtat tagaccaagt accaaccgtt ctgtaccatc
      841 agtaacagtt gttgataatt taccaggctt tacacttatc aatggtggta aagtaggtgt
      901 gtttagtcat gcgatggtta gaacaagtat gtttgattca ggagatgcta agaactatca
      961 agcacaagga aatgtaattg cattaggtcg tattaaggga aatgatacaa atgatcatgg
     1021 tgattttaat ggaattgaaa aatctttaac agtaaatcca aattctgaat taattttcga
     1081 atttaatacg atgactacaa aaaactatca aggtgtgaca aatttaataa ttaaaaatgc
     1141 tgataatgat actgttattg cagaaaaatc agtagcttat ggtccgattt ggcgtttgtt
     1201 taaagtgcct gaaaatgtta gtcacttaaa aattcaattt gtacctaaaa atgatgcaat
     1261 aacagatgcg cgtggcattt atcaactaag agacggttat aaatactatg actttgttga
     1321 ctcaattggt ctacattctg gttcgcatgt ctatgttgaa agacgaacaa tggaacccac
     1381 agctactaat aataaagaat ttacagtcac aacttcatta aagaataatg gtaacttcgg
     1441 tgcttcattt aatacagatg attttgtata taaagttcaa ttacctgaag gggtagaata
     1501 tgttaataac tcattgacta aagattttcc aagcagtaat tcaggtgttg atatgaatga
     1561 ttttaatgtg acgtatgatg cggcgaatcg agtgataaca attaaaagta ctggtggagg
     1621 ttcaggtaac tcgccggcac gattaatgcc tgataaaata ttggatttaa agtataagtt
     1681 gcgtgttaat aatgtaccga caccaagaac agtaacattt aatgatacat taacatataa
     1741 aacatataca caagatttta ttaattcacc tgctgaaagt catactgtaa gtacaaatcc
     1801 atatacaatc gatatcatca tgaataaaga tgcattacaa gccgaggttg atagacgcat
     1861 tcaacaagct gattatacat ttgcatcatt agatatcttt aatgatctta aaagacgtgc
     1921 acaaacgatt ttagatgaaa atcgtaacaa tgtaccatta aataaaagag tttctcaagc
     1981 agatattgat tcattaacta atcaaatgca acatacgtta attcgaagtg ttgatgctga
     2041 aaatgcagtt aataaaaaag ttgaccaaat ggaagattta gttaatcaaa atgatgaatt
     2101 gacagatgaa gaaaaacaag cagcaataca agttatcgag gaacataaaa atgaaataat
     2161 tggtaatatt ggtgaccaaa cgactgatga tggcgttact agaatcaaag atcaaggtat
     2221 acagacctta agtggggata ctgcaacacc ggttgttaaa ccaaatgcta aaaaagcaat
     2281 acgtgataaa gcaacgaaac aaagggaaat tatcaatgca acaccagatg ctactgaaga
     2341 cgagattcaa gatgcactaa atcaattagc tacggatgaa acagatgcta ttgataatgt
     2401 tacgaatgct actacaaatg ctgacgttga aatagctaaa aataatggca tcaatactat
     2461 tggagcagtt gttcctcaag tgacacataa acaagctgca agagatgcaa ttaaccaagc
     2521 aacagcaacg aaaagacaac aaataaatag taatagagaa gcaactcagg aagagaaaaa
     2581 tgcagcattg aacgaattaa ctcaagcaac caaccatgct ttagaacaaa tcaatcaagc
     2641 aacaacaaat gctgatgttg ataacgccaa aggagatggt ctaaatgcca ttaatccaat
     2701 tgctcctgta actgttgtta agcaagctgc aagggatgct gtatcacatg atgcacaaca
     2761 acatatcgca gaaatcaatg ctaatcctga tgcgactcaa gaagaaagac aagcagcaat
     2821 tgacaaagtg aatgctgctg taactgcagc aaacacaaac attttaaacg ctaataccaa
     2881 tgctgatgtt gaacaagtaa agacaaatgc gattcaagga atacaagcaa ttacaccagc
     2941 tacaaaagta aaaacagatg caaaaaatgc catcgataaa agtgcggaaa cgcaacataa
     3001 tacgatattt aataataatg atgcgacgct cgaagaacaa caagcagcac aacaattact
     3061 tgatcaagct gtagccacag cgaagcaaaa tattaatgca gcagatacga atcaagaagt
     3121 tgcacaagca aaagatcagg gcacacaaaa tatagttgtg attcaaccgg caacacaagt
     3181 taaaacggat gctcgcaatg ctgtaaatga taaagctcga gaggcgataa caaatatcaa
     3241 tgctacacct ggcgcgactc gagaagagaa acaagaagcg ataaatcgtg tcaatacact
     3301 taaaaataga gcattaactg atattggtgt gacgtctact actgcgatgg tcaatagtat
     3361 tagagacgat gcagtcaatc aaatcggcgc agttcaaccg catgtaacga agaaacaaac
     3421 tgctacaggt gtattaaatg atttagcaac tgctaaaaag caaaaaatta atcaaaacac
     3481 aaatgcaaca actgaagaaa agcaagtggc tttaaatcaa gtggatcaag agttagcaac
     3541 ggcaattaat aatataaatc aagctgatac aaatgcggaa gtagatcaag cgcaacaatt
     3601 aggtacaaaa gcaattaatg cgattcagcc aaatattgtt aaaaaacctg cagcattagc
     3661 acaaatcaat cagcattata atgctaaatt agctgaaatc aatgctacac cagatgcaac
     3721 gaatgatgag aaaaatgctg cgatcaatac tttaaatcaa gacagacaac aagctattga
     3781 aagtattaaa caagctaaca caaatgcaga agtagaccaa gctgcgacag tagcagagaa
     3841 taatatcgat gctgttcaag ttgatgtagt aaaaaaacaa gcagcgcgag ataaaatcac
     3901 tgctgaagtg gcgaagcgta ttgaagcggt taaacaaaca cctaatgcaa ctgacgaaga
     3961 aaagcaggct gctgttaatc aaatcaatca acttaaagat caagcaatta atcaaattaa
     4021 tcaaaaccaa acaaatgatc aggtagacac aactacaaat caagcggtaa atgctataga
     4081 taatgttgaa gctgaagtag taattaaacc aaaggcaatt gcagatattg aaaaagctgt
     4141 taaagaaaag caacagcaaa ttgataatag tcttgattca acagataatg agaaagaagt
     4201 tgcttcacaa gcattagcta aagaaaaaga aaaagcactt gcagctattg accaagctca
     4261 aacgaatagt caggtgaatc aagcagcaac aaatggtgta tcagcgatta aaattattca
     4321 acctgaaaca aaagttaaac cagctgcacg tgaaaaaatc aatcaaaaag cgaatgaatt
     4381 acgtgctaag attaatcagg ataaagaagc aacagcagaa gaaagacaag tagcactaga
     4441 taaaatcaat gaatttgtaa atcaagccat gacagatatt acgaataata gaacaaatca
     4501 acaagttgat gatacaacaa gtcaagcgct tgatagcatt gctttagtag cgcctgaaca
     4561 tattgttaga gcagctgcta gagatgcagt aaagcaacaa tatgaggcta aaaagcaaga
     4621 aatagagcaa gctgaacatg cgactgatga agaaaaacaa gttgctttaa atcaattagc
     4681 gaataatgaa aaacttgcat tacaaaacat caatcaagca gtaacgaata atgatgtgaa
     4741 acgtgttgaa acaaatggca ttgctacact aaaaggtgta caacctcata ttgtaattaa
     4801 gcctgaagca caacaagcaa taaaagcaac tgcagaaaat caagtagaat caataaaaga
     4861 tacaccacat gcaacagttg atgaattaga tgaagcgaat caattaatta gcgacacact
     4921 caaacaagcg caacaagaaa tagaaaatac aaatcaagat gctgctgtta ctgatgttag
     4981 aaatcaaaca atcaaggcaa ttgagcaaat aaaacctaaa gtaagacgta aacgagctgc
     5041 gcttgatagc attgaagaaa ataataaaaa tcaactcgat gcaatccgaa atacgttgga
     5101 tactactcaa gatgaaagag atgttgctat tgatacttta aataaaattg taaatacaat
     5161 taaaaatgac attgcacaaa acaaaacgaa tgcagaagtg gatcgaactg agactgatgg
     5221 caacgacaac atcaaagtga ttttacctaa agttcaagtt aaaccagcag cgcgtcaatc
     5281 tgttggtgta aaagccgaag ctcaaaatgc actaatcgat caaagcgatt tatcaactga
     5341 agaagaaaga ctagctgcta aacatttagt agaacaagca cttaatcagg ctattgatca
     5401 gatcaatcat gcagataaga ctgcccaagt taatcaagat agtatagatg ctcaaaatat
     5461 tatttcaaaa attaaaccag cgacaacagt taaagcaaca gcattacaac aaattcaaaa
     5521 tatcgctaca aataaaatta atttaattaa agcaaataac gaagcgacag atgaagaaca
     5581 aaatattgca atagcacaag ttgaaaaaga gttaattaaa gctaaacaac aaattgctag
     5641 tgcagtgact aatgctgatg tggcatattt attgcatgat gagaaaaacg aaattcgtga
     5701 aatcgaacct gttattaaca gaaaggcgtc tgctcgagaa caattgacaa cattattcaa
     5761 cgataaaaaa caagcaattg aagcgaatat tcaagcaacg gtagaagaaa gaaatagtat
     5821 attagcacag ttacaaaata tttatgacac tgctattgga caaattgatc aagatcgtag
     5881 caatgcacaa gttgataaaa cagcatcatt aaatctacaa acaatacatg atttagatgt
     5941 acatcctatt aaaaagccag atgctgaaaa aacgattaat gatgatcttg cacgcgtcac
     6001 tgctttagtg caaaattatc gaaaagtaag taatcgtaat aaggctgatg cattaaaagc
     6061 tataactgct ttaaaattac aaatggatga agaattaaaa acagcacgca ctaatgctga
     6121 tgttgatgca gttttaaaac gatttaatgt tgcattaagc gatatagaag cagtaattac
     6181 tgaaaaagaa aatagcttac tgcgaattga taacattgct caacaaacat atgcgaaatt
     6241 caaagcgatc gcaacaccag aacaattagc taaagtaaaa gtattaattg atcaatatgt
     6301 tgcagatggc aatagaatga ttgatgaaga tgcgacatta aatgacatca aacaacacac
     6361 gcaattcatt gttgatgaaa ttttagcaat taaattacca gctgaagcga cgaaagtatc
     6421 accaaaagaa attcagccag ctccaaaagt ttgtacgcct attaaaaaag aagagacaca
     6481 tgaatcgcgc aaagttgaaa aagaacttcc aaatacaggt tctgaaggaa tggatttacc
     6541 attgaaagaa tttgcactga ttacaggtgc ggctttgtta gctagaagac gtactaaaaa
     6601 cgaaaaagaa tcataattaa cgaaatgacc ttaatataac gacacttttt agtgactcag
     6661 ttatattaag gtcattttta attgaatgaa aaagttgatc tactaaagag aatcgaacca
     6721 aattttatac tattattcca ttgatgtatg tgagtaatga ccggcatata atccagcgac
     6781 atgtcctgtt acgagtgcac ttgtaatatt ataaccacca gtataaccat gtatatctaa
     6841 tacttcacca cataaaaata atcccggaac taatttagac atcattgttt taggttgaat
     6901 ttctttaagt gacacaccac cacctgtgac aaatgcctta tctataggta atgtcccgtt
     6961 taccttaaat acaaaccctt taaacatatt tactaagtcg ttcaattgtt gatttgataa
     7021 gtgatgtgat gtggtatttt catcgattcc agcttgttcc agcatgaaca gtaagtagcg
     7081 ctcttcaatt agaccatgca aactgttttt aatgatttta tctggtgtgt ccgataataa
     7141 tgatgtgatg tgttgtttta attgttcatg gtttaattca ggaaatgcat cgattgccat
     7201 agaaatgtgc tgtgtctttt gatttttttg ttctttataa acaaactgac tacatcttaa
     7261 tgcagctgga ccactgatac caaaatgagt aaataacata tccatttgat gactgatgcg
     7321 ttttttacca tttttcttaa gtactgacaa ttcaacatct tttaaactta aaccttttag
     7381 acgattggat ttgatgaaag gttcagctga tgtaattgga acttcggtcg ggaataactc
     7441 agtaatggta tgacctaaat cttgtgcgaa cttataacca tcaccagttg aaccagtttg
     7501 agggacactt gtaccacctg tagcaatcac tagcgaatgg ctttcataac tattattttg
     7561 agtatgtaca gtgaaagttt ggtctgtatt aacttcgatt ctactaacag cttcttcttc
     7621 tttaatcgtt acatgttggc gttcgatagt tgtcactaat gtatcaacca cgtcttgtgc
     7681 tttgttggaa actggaaaca tacgcccgtg atcttcttct tttaatttaa cacccctaga
     7741 ctcaaaaaaa tctatgatgg attcattatc aaaaattgaa aagggactat ataaaaattt
     7801 cccatttcca ggaatgttct taataatttc agcatatggt aatcgattag ttacgttaca
     7861 tctaccgcca ccagatattt tgagtttacg acctagacct ttcttttttt caatgagtaa
     7921 cacactgcta ctttgttcgc ttgcagctac tgccgccatt aagccgctag gtccgcctcc
     7981 gataataatt gtttgataca tatacgcttc ctccaaattt gttactggac attcaattgc
     8041 tattttatag tactttttta aactttgcta tatctcttgt gttatgagat gacaattatg
     8101 ttgaatatat ttataatgtg aacttcataa aaaatgaaga tgtatgtgta tgataagaat
     8161 gatttttcta ctgttatgcg atgt
//
LOCUS       DAHHSC010000109         8165 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.110, whole genome shotgun sequence.
ACCESSION   DAHHSC010000109 DAHHSC010000000
VERSION     DAHHSC010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 8165)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8165)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8165
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.110"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            complement(220..408)
                     /locus_tag="NBI57_001766"
     CDS             complement(220..408)
                     /locus_tag="NBI57_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1822703.1"
                     /translation="MNKDTVLDKGYVIATILNVFFLLGLIFISHFENLYILILYVILM
                     GINAIYLVVKVLNFKKNN"
     gene            complement(979..1125)
                     /locus_tag="NBI57_001767"
     CDS             complement(979..1125)
                     /locus_tag="NBI57_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001092934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1822704.1"
                     /translation="MNTKILTGMTGSSLERKINNFINDNQIEVIDIKFSSSVFYFGVM
                     IIYK"
     gene            complement(1130..1455)
                     /locus_tag="NBI57_001768"
                     /pseudo
     CDS             complement(1130..1455)
                     /locus_tag="NBI57_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002483623.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
     gene            complement(1448..2392)
                     /locus_tag="NBI57_001769"
     CDS             complement(1448..2392)
                     /locus_tag="NBI57_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001132443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HCD1822705.1"
                     /translation="MPNFEKYNLSQVKTERFYQLPKYLFEDAYFKKMSAEAKIMYALL
                     KDRFELSIQNEWVDRNNNIYFIFSNKHLCEYLGYGEQKIIKLKKELVNFNLLTQERVG
                     LNKPNRLYLLKPNYDIEASLTKELPNSQFQNNESGSSRTVNLSGQELPNSQSNDTELN
                     NTNYIENEDNDTYDMNDIISNTLINNNHHSNHTNHQKTEFNNDALKFQVLEELPQQLQ
                     DYLSKFEIREIRIIKSVLLKGKKSFNTSHDTYYRLEDVEFEIVSVLKRFKAMLLQKNE
                     TVEAMQGYLMQSIKAELEETHALYMRRKNMKQYNIFNQ"
     gene            2836..3606
                     /locus_tag="NBI57_001770"
     CDS             2836..3606
                     /locus_tag="NBI57_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001813127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HCD1822706.1"
                     /translation="MKTIKMVADELNVTKQTIVNNAKNLNISFKKENGINYINDNDCL
                     KIIEKITKKERTMQNKESIKKERFNENTAEFEKHIYNNSNSFESLKTKVNELEKQIEI
                     FETRAKNDEKYIENLTKQLDQQNSNVNTLNKLLENQQILALESNKKIQKLENQLEEER
                     QLNYSFDTATNDRQSINPQEATFTEEYQNSNQQQKKIQPIEAELNHKGITKAKKDDSA
                     NEDVLKRDDKLSEEIESGPEDREVETPKKGFWSRLFGS"
     gene            3710..4418
                     /locus_tag="NBI57_001771"
                     /pseudo
     CDS             3710..4418
                     /locus_tag="NBI57_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009384749.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
     gene            5129..5806
                     /locus_tag="NBI57_001772"
     CDS             5129..5806
                     /locus_tag="NBI57_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000559653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1822707.1"
                     /translation="MIACISLGISIFALGISIYTVFYQRKKDNNLLRPQLFLESQNKQ
                     TVDYIGINLDIGGYEKLQEYDVLLTFINTTQVRIIDLSVSIDVRKNDTFDYLLSKALE
                     EKENNFDIEKDMFGNDTYGWDKVFGLEETTKEKISVLDGGDSFELRLPSIFSALLLGA
                     MYLNRNDFTESNFTIDFDVKILYNHDFANKGKYVCVNKTVSVNITYERAIQGPWVVYD
                     SIIFDKL"
     gene            6864..7676
                     /locus_tag="NBI57_001773"
     CDS             6864..7676
                     /locus_tag="NBI57_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1822708.1"
                     /translation="MSLKDIEFWMSLYDILKDVWFLILIPIVISIVKLLNKNDLYQTR
                     NVEKIKMVLKNTRLFILITIGALIYLVSMIFFSLSAYPKDEVMEYISKWDNIGSFTIL
                     IIMYAMLFPIVTIPFLGERRNKRFFLEKNNNEVIEREIIDRVYINGKDKLILKDGEGI
                     QTEKNVTDIDNLTFKIQDKKSWIAMEDIQKIFLKFQDRSKKKRISIFIFICSIPTVYL
                     AWNLPSIIDMFTKLQKWTDIFMYISFFTLPLILIIELYYVVYIVYKSLFKKV"
BASE COUNT         3116 a          982 c         1220 g         2847 t
ORIGIN      
        1 ctccaagttt agttaaggct tcttctcaac gcacaataaa ttttctcggc ataaatgcgt
       61 cgtctatttt ttattatctt catgataatc atatttttct tccatctcgt caaaatcatc
      121 atctttatag aaaaacaaaa agtaagcaac aacaccaata ggaataaaaa aacagtgtga
      181 aaacaagaag aaactcgttt caaaatcctc tcaaatattc taattattct ttttgaaatt
      241 caaaacctta acaaccaaat aaatagcatt tattcccatc aaaataacat acagaattaa
      301 tatatataaa ttttcaaaat gagatataaa aattaatcca agtaaaaaga aaacatttaa
      361 aatagttgct ataacgtaac ctttatctaa cactgtgtct ttattcaaaa taaagactcc
      421 ttttttaaaa aactttatca caagacttac tcaaattata aataccttta ttttgaattt
      481 taagggggta ttttaaagat ttaggggtaa atcatatagt tttcctacat aatctcttaa
      541 aacgcaaata tgagccaaat acatatatgc tattttcaaa aacaaaattt taaataaaac
      601 caatgactat ttttttaaac actgcccatt tacatgcaaa ttaaaagtta gcataaaatc
      661 ctttaaaact tcccccatgt ttttctaaat tcctgcaaga cattttttat caaaatcgag
      721 aatttttatt ttttctctaa gaactccgta actacgtaag agcataaggg aaatttgacc
      781 ccatgtatac atggggtatc tggcaaagcc agacaattca aagcttcaac aatattttta
      841 tgtggggtca taaaaagctt aagtgtcaca cttttgtgac actacataca gcatttgtca
      901 caaaaataag acagtgcttt tttataaaaa aacagtaaaa aaagacatca gtcatcacta
      961 atgtcttaaa ctctttaatt atttataaat aatcattacc ccaaaataaa atacagaact
     1021 tgaaaactta atatcaatga cttcgatttg gttatcatta ataaaattat tgatttttcg
     1081 ttctaaagaa cttccagtca ttccagttaa aattttagtg ttcatatttt taactcctta
     1141 aattttttaa ataataattt tgcttttcga aattccaaat tgctgtaatc gaatgtgctt
     1201 tttcttcttc gtctgtcgtt ttatcttcat cacttgtatt aatcaaatta ccatcttcgg
     1261 catcgtctaa gtttaaaatt ttatgctttt ctttaagtaa tccaccataa cttaacatac
     1321 gttttcgata caaacctttt ctagatcact taaaatttct tgattttttt cgtcgtcatc
     1381 agttaaaaag tcagatgatt taacggaata ttttgatgtc tctttgattg ttataaaatc
     1441 atttgaatta ttgattaaag atattatatt gtttcatgtt tttacggcgc atataaaggg
     1501 catgtgtttc ttcaagttca gctttaatgg attgcattaa ataaccttgc atagcttcaa
     1561 cggtttcatt cttttgtagc aacatcgctt taaaacgttt caatacactt acaatttcaa
     1621 actcaacatc ttctaaacga taataagtat catgtgaagt attaaatgac tttttccctt
     1681 tgagtaatac gcttttaata atccgaattt ctctaatttc aaatttactt aaatagtctt
     1741 gaagttgttg tggcaattct tcgagtactt ggaacttaag ggcgtcatta ttaaattcag
     1801 ttttttgatg atttgtatga ttcgaatgat gattattatt tattaaagta ttagatataa
     1861 tatcattcat atcatacgta tcattatcct cattttctat ataattagta ttatttaact
     1921 cagtatcatt agactgtgaa tttggaagtt cttgaccact taaattcaca gttctagaac
     1981 ttccagattc attgttctgg aactgtgaat ttggaagttc cttggtaaga cttgcttcaa
     2041 tatcataatt tggttttaat aaatataatc tatttggttt attaaggcca acacgctctt
     2101 gagttaataa attaaaattt actaactctt ttttcaattt tatgattttt tgttctccat
     2161 aacctaagta ctcgcacaaa tgtttattac tgaaaataaa gtaaatgtta ttatttctat
     2221 ctacccattc attttggatg gataattcga aacgatcttt taataatgca tacataattt
     2281 tggcttctgc tgacattttc ttaaaatatg catcttcgaa caaatattta ggcagttggt
     2341 aaaatctttc agtttttact tgtgataaat tgtatttctc aaaattgggc ataataaaaa
     2401 cctcatttcc actttttgga tagaggtaat aaaacaattt ttacgtaggt tatatgtttt
     2461 gtacttgcac ttatatgatt agatatatat aatacaagta tcaaaaaatt atataaacag
     2521 acgaatgcga atcgttttat taccgagagt cgatgctacg ccaatagtat cgactctttt
     2581 aatattcaca aagtaatatt aacactttta agattgtata taaagctttt taagtttagt
     2641 tgaaagaaca ttgtaacttt aaaatgataa aaattcacta atttagcacc taattagccg
     2701 ttaaaacctt ctataggtgg ctaattaggt gctaaattag tgaaataggt agtaaattta
     2761 gatatcgaaa gatgaaaaaa tatttgttag gtggtaaaat ttaggtacca atttatgtac
     2821 ctaaggatga atgttatgaa aactattaaa atggttgctg atgaattgaa tgtaactaaa
     2881 caaactattg ttaataatgc taaaaactta aatatatctt ttaaaaaaga aaatggaatt
     2941 aattatatta atgataatga ttgtttaaaa attatagaaa agatcactaa gaaagaaagg
     3001 acaatgcaaa ataaagaatc aataaaaaaa gaaagattta atgaaaatac tgcagagttt
     3061 gaaaagcata tatacaataa ttcaaatagt tttgagtcat taaaaacaaa agtaaatgaa
     3121 ttagaaaagc aaatagaaat ctttgaaact agagctaaaa atgatgaaaa atatattgag
     3181 aatttaacta aacaactaga tcaacaaaat agcaatgtta atacattaaa taaactgttg
     3241 gagaatcaac aaatattagc tttagaaagt aataaaaaga ttcaaaaact agaaaaccaa
     3301 ttagaagaag aaagacagct taattactct tttgatacag cgactaatga tagacaaagc
     3361 attaatccgc aagaagcaac ttttactgaa gaatatcaaa atagcaacca gcaacaaaag
     3421 aaaatacaac caatagaagc agaattaaat cataagggca taactaaagc gaaaaaggat
     3481 gacagtgcaa acgaagacgt attaaagaga gatgataaac tatctgaaga aatagagagt
     3541 ggacctgagg acagggaagt agaaacacct aaaaaggggt tttggagtcg tttgtttggt
     3601 agctaaaata attaattagg cactaaaagt ttataacttt tagtgccttt tatatagtga
     3661 gaacttctgg taatcaaagt tatgtccagt ttgtaattgg agacgttcta tgaaaaatag
     3721 ttatcaatcc caaaaagtga ttgaagaagt gtttaaagaa aagccgaatt ctcgttggtt
     3781 gtttcttaca ttatcaatac gaaatgcaat aggtggagat acgttagaac aaaatttaac
     3841 acatttaact gagtcgttta gaagattatt taaatataaa aaaatcagta aaaatttaat
     3901 tggctttatg cattcaactg aagtaacagt taataaaaat gacggtagtt ataatcaaca
     3961 tatgcacgtt ttgttgtgtg ttgaaaatgc gtattttaga aagaaaaaat atattacaca
     4021 aacagaattg gttgatttat ggcaacaagc attaaaagta aattatcgac ctgtcgtaaa
     4081 tattaaagcg atcaaaccaa aaaggagata aagatattgc gtctgctatc aaggagacat
     4141 caaaatattc cgttaaatca tctgactttt taactgatag cgatgaaaaa aatcaagaaa
     4201 ttatcagtga tctagaaaaa ggtttgtatc gaaaacgtat gttaagttat ggtagattgc
     4261 ttaaacaaaa gcataaaatt ttaaatttag ataatgttga agatggtaat ttaattaata
     4321 caagtgacga agataaaaca acagacgaag aggaaaaagc acattcaatt acagcaattt
     4381 ggaatttcga aaagcaaaat tattatttaa gacattagtg atgactgatg tcttttttat
     4441 tgattttttt gtaaaaaagt actgtcttat ttttgtgaca aatgctgtat gtagtgtctc
     4501 aaaactgtga caaccgcaat atattgtgtc acaaaagcgt gacactacag ctttttatga
     4561 tatcacttta aaattactta aaacccttag aatgtctggc tttgccagac ctatcatttt
     4621 tgaatgatag caaattctcc ttatgctctt acagagttct tagagataaa ttaaaaattc
     4681 tcgatttttg ataaaaaacg ctctgcagga atttagaaaa acacgggaaa gttttaaagg
     4741 attttatgct aatttttaat ttgcatgtaa atggacagtg ttacaaaata gtcattgttt
     4801 ttgctgaaat ttttgtttgt gaaattagca tatatgtatt tggctcatat tttcgtttta
     4861 aaagcgtata taagttttgg atagaaaaaa tatatgattt acccctaaac ctttaaaatg
     4921 ccccttaaaa ttcaaaataa gggcatttaa cattgtaata aggattgcta ttttttataa
     4981 aaggagagat atcatgaaga ttaaaacttt cgatactttt actcatcgtc aataaagcaa
     5041 ttggaataaa tttttaataa tttgactgct tattcttcta aaaaaattga tatgattatt
     5101 taaactaatt taaaggagga ttattgattt gattgcttgt atttctttag gaatatctat
     5161 ttttgcttta ggtatttcaa tatatactgt gttttatcag agaaaaaaag ataataatct
     5221 tttaagacct caactttttt tagaatctca aaataagcaa actgtagatt acataggtat
     5281 taatttagat attggaggtt acgaaaagct acaggaatat gatgttttat taacatttat
     5341 taatacaacg caagtaagaa ttatagattt gtctgtatct attgatgtga ggaaaaatga
     5401 tacttttgat tatttattaa gtaaagcatt agaagaaaaa gaaaacaatt ttgatataga
     5461 aaaagatatg tttggtaatg atacttatgg ttgggataaa gtttttggtt tggaagaaac
     5521 gacaaaagaa aaaatatcag ttcttgatgg cggagattca ttcgaattga gattaccaag
     5581 tattttttca gctttattat taggtgccat gtatttaaat agaaatgact ttacagaatc
     5641 taactttaca atagattttg atgtaaaaat tttatataat catgattttg ctaataaagg
     5701 taaatatgtt tgtgtaaata agactgtttc agtaaatatc acttatgaaa gagcgattca
     5761 aggaccatgg gttgtttatg attctattat ttttgataaa ttgtgaataa tgataaagcg
     5821 ttttttggaa cgagtgaaac gagtttcttc ttgtcttgat actatataga aataacaagc
     5881 tattataaat agtataacgc tataaaaagg gaatgcgtct acgaagataa acataaatat
     5941 gtttgatttt aaagtgatcg taataaatga tttgtataaa atcatattta gaaaatgtaa
     6001 tccaatcttg tttattaacc aaattaaaat gagatataag ttgggaaaat tgataaatac
     6061 atgttctatt ttcatcaggt aggtaacaat attttatggt tttattttga acacttcttt
     6121 tgttatagat acatcttaaa tatagtgtaa aaattagtgc ttttaacatt tgatttgata
     6181 attttttctt acatggtatg aaaaaattat atgtatatta tgagcgtttt agtaaaaacg
     6241 tgaaaatcag taaaaatctt gtaagagcat aaggggaatt tgtacccatt tgaaaatggg
     6301 tgatctggcg aagccagata tttcaaggga tctagtattt tttgatgtgg gggccaaaat
     6361 tttgttagtg tcacaaaagt gtgacacctt tttaatgaga aaagttgggt tattttggct
     6421 aacaaagaac tgaaaattag aaatgtagat agtgaattgc tagattgatt aagaggatta
     6481 gctaaagaga aaaacatatc gagaaatgaa ttaatatcag aaacattaga acagctatat
     6541 ccactggaaa gttgtcaaaa atcatatgca gaacaatcat atcagcaagc gcaaaataca
     6601 aaaaatttaa aagcagttgc gagtaaacaa gacaaaattt tagcgttact tgaatcagta
     6661 gattattaaa catgataaca acagttaaaa aagtttgagc tattttatag ttaaaatgta
     6721 ttgaaattca aaacattcat gcgtggaata aataacaaag atatatccat tttaacgcca
     6781 aaaggaaact tgattgagga tataaataaa ggtataaaag ataaggatta acaatttatt
     6841 tatatcataa tgaggtgtga aaattgagtt taaaggacat agaattttgg atgagtttat
     6901 acgatattct taaagatgtg tggtttttaa ttttaattcc tattgttatt agtattgtaa
     6961 aactattgaa taaaaatgac ttatatcaga ctcgaaatgt tgagaaaata aagatggtat
     7021 taaaaaatac gcggttattt attttgatta ctattggagc actgatatat ttggtgtcga
     7081 tgatattttt ttcattgagt gcctatccta aagacgaagt tatggaatat atatctaaat
     7141 gggataacat tggttcattc accattttaa tcattatgta tgccatgttg tttccaatag
     7201 ttacaattcc ttttcttgga gaacgtagaa ataagcgatt ctttttagaa aagaataata
     7261 acgaggttat agaaagagaa atcattgacc gtgtctatat caacggaaaa gataaattaa
     7321 ttttaaaaga tggcgaaggt attcaaactg aaaagaatgt tacagatata gataatttaa
     7381 catttaaaat acaagataaa aaatcatgga tagcaatgga agatatacaa aaaatctttt
     7441 taaaatttca agacagaagc aaaaaaaaga gaatctccat ttttattttt atatgtagta
     7501 ttccaacagt ttatttagct tggaatttac cgagcataat tgatatgttt actaaattac
     7561 aaaaatggac agatatattt atgtatatca gtttttttac tttgccattg atactgatca
     7621 tagaattata ttacgttgtc tacattgttt ataaaagttt atttaaaaag gtctaatttt
     7681 gataattgaa aaatagtcgt gtaagttaca caagtgtaat taacacaagt ataatttaca
     7741 cttgtgtata aagcatatat gagatgtaag ttatttaaac attatgatga gttacataaa
     7801 gttatattca gagaaaataa aggaacagta gtaaaactca aaaaggttta tcaacaagag
     7861 ataccttaat aagcggatca attctactaa tggtaatagt agtttggtgg gattgggatg
     7921 ttttaattaa gtatttttca gattctttat tactgtctag atctcttact ctataaatac
     7981 ctaatttatt tttgaaaaga ctttatactg aactagggat aaattgaaca ggtgatttta
     8041 taattaagat ggtgatgcca tatacttttt tatgtataac tacaaagaat tggagagaat
     8101 ggttttcata attagttctt atgggtagtg attttgtata gaatacgtat actttgtaaa
     8161 attga
//
LOCUS       DAHHSC010000110         8046 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.111, whole genome shotgun sequence.
ACCESSION   DAHHSC010000110 DAHHSC010000000
VERSION     DAHHSC010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 8046)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8046)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8046
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.111"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            <1..88
                     /locus_tag="NBI57_001774"
     CDS             <1..88
                     /locus_tag="NBI57_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1822709.1"
                     /translation="IDVYRVGRENIVIAVYNEHIKLLFILIS"
     gene            complement(166..1671)
                     /locus_tag="NBI57_001775"
     CDS             complement(166..1671)
                     /locus_tag="NBI57_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HCD1822710.1"
                     /translation="MTQQNSHGNQIQDIPQTGFFGHPRGLGVLFFVEFWERFSYYGMR
                     ALLIFYMYFAVTDNGLGIDKTTAMSIMSVYGSLIYMTSIPGGWIADRITGTRGATLLG
                     AVFIIIGHICLSLPFALIGLFTSMFFIIIGSGLMKPNISNIVGRLYPENDRRMDAGFV
                     IFYMSVNMGALLSPIILQHFVNVKNFHGGFLIAAVGMALGLVWYVLFNRKNLGSVGMK
                     PTNPLTPAEKKKYGLIIGSVVLAIVLIIVIGALTNSLSFNLVSNTVLVLGIALPIIYF
                     TLIIRSKDVTDTERSRVKAFIPLFILGMVFWAIQEQGSNVLNIYGIEHSDMKLNLFGW
                     KTNFGEAIFQSINPLFILLLAPIISLLWQKLGTKQPSLPVKFAIGTFLAGASYILIGI
                     VGYASGSSNFSVNWVILSYIICVIGELCLSPTGNSAAVKLAPKAFNAQMMSIWYLTNA
                     SAQAINGTLVKLIEPLGQTNYFIFLGVVAIIVTTIVLAFSPLIIKAMKGIR"
     gene            complement(2012..2512)
                     /gene="queF"
                     /locus_tag="NBI57_001776"
     CDS             complement(2012..2512)
                     /gene="queF"
                     /locus_tag="NBI57_001776"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HCD1822711.1"
                     /translation="MAHGRQQDELQDITLLGNQDNTYNFDYRPDVLESFDNKHQGRDY
                     FVKFNCPEFTSLCPITGQPDFATIYISYIPNVKMVESKSLKLYLFSFRNHGDFHEDCM
                     NIIMNDLIELMDPHYIEVWGKFTPRGGISIDPYTNYGRPNSKYEKMAEHRLMNHDLYP
                     EKIDNR"
     gene            complement(2532..3398)
                     /locus_tag="NBI57_001777"
     CDS             complement(2532..3398)
                     /locus_tag="NBI57_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001594836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HCD1822712.1"
                     /translation="MNPKVKGIIAILISAIGFSFMSVFFRLAGDLPVFQKSLARNLVA
                     MFIPLFFLYKYHQPMFGRLSSQPLLITRSTLGLIGVLLNIYAIDHMVLSDADSLMKLN
                     PFWTILLSIVFLHEKVRKYQITAMIIAILGMLLIVKPEFSSSMIPSLAGLFSGIFAAS
                     AYTCVRALSSREAPYTIVFYFSLFSVIVLIPFTAYTYEPMSQMQILYLLGAGLAAAVG
                     QIGVTLAYSFAAAKDISIFTYASIIFTAILGFVLFGESPDFYATLGYVVIIGASYYMF
                     EKARRDAKIIKK"
     gene            4199..4597
                     /gene="nrdI"
                     /locus_tag="NBI57_001778"
     CDS             4199..4597
                     /gene="nrdI"
                     /locus_tag="NBI57_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HCD1822713.1"
                     /translation="MKIIYFSFTGNVRRFIKRTELENTLEITAENCMEPVHEPFIIVT
                     GTIGFGEVPEPVQSFLEVNHQYIRGVAASGNRNWGLNFAKAGRTISEEYNVPLLMKFE
                     LHGKNKDVIEFKNKVGNFNENHGREKVQSY"
     gene            4560..6665
                     /gene="nrdE"
                     /locus_tag="NBI57_001779"
     CDS             4560..6665
                     /gene="nrdE"
                     /locus_tag="NBI57_001779"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000855513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HCD1822714.1"
                     /translation="MKTMEEKKYNHIELNNEVTKRREDGFFSLEKDQEALVAYLEEVK
                     DKTIFFDTEIERLRYLVDNDFYFNVFDIYSEADLIEITDYAKSISFNFASYMSASKFF
                     KDYALKTNDKSQYLEDYNQHVAIVALYLANGNKAQAKQFISAMVEQRYQPATPTFLNA
                     GRARRGELVSCFLLEVDDSLNSINFIDSTAKQLSKIGGGVAINLSKLRARGEAIKGIK
                     GVAKGVLPIAKSLEGGFSYADQLGQRPGAGAVYLNIFHYDVEEFLDTKKVNADEDLRL
                     STISTGLIVPSKFFDLAKEGKDFYMFAPHTVKEEYGVTLDDIDLEKYYDDMVANPNVE
                     KKKKNAREMLNLIAQTQLQSGYPYLMFKDNANRVHPNSNIGQIKMSNLCTEIFQLQET
                     SIINDYGIEDEIKRDISCNLGSLNIVNVMESGKFRDSVHSGMDALTVVSDVANIQNAP
                     GVRKANSELHSVGLGVMNLHGYLAKNKIGYESEEAKDFANIFFMMMNFYSIERSMEIA
                     KERGIKYQDFEKSDYANGKYFEFYTTQEFEPQFEKVRELFDGMAIPTSEDWKKLQQDV
                     EQYGLYHAYRLAIAPTQSISYVQNATSSVMPIVDQIERRTYGNAETFYPMPFLSPQTM
                     WYYKSAFNTDQMKLIDLIATIQTHIDQGISTILYVNSEISTRELARLYVYAHYKGLKS
                     LYYTRNKLLSVEECTSCSI"
     gene            6783..7754
                     /gene="nrdF"
                     /locus_tag="NBI57_001780"
     CDS             6783..7754
                     /gene="nrdF"
                     /locus_tag="NBI57_001780"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HCD1822715.1"
                     /translation="MIAVNWNTQEDMTNMFWRQNISQMWVETEFKVSKDIASWKTLSE
                     AEQDTFKKALAGLTGLDTHQADDGMPLVMLHTTDLRKKAVYSFMAMMEQIHAKSYSHI
                     FTTLLPSSETNYLLDEWVLEEPHLKYKSDKIVANYHKLWGKEASIYDQYMARVTSVFL
                     ETFLFFSGFYYPLYLAGQGKMTTSGEIIRKILLDESIHGVFTGLDAQHLRNELSESEK
                     QKADQEMYKLLNDLYLNEESYTKMLYDDLGITEDVLNYVKYNGNKALSNLGFEPYFEE
                     REFNPIIENALDTTTKNHDFFSVKGDGYVLALNVEALQDDDFVFDNK"
     gene            7935..>8046
                     /locus_tag="NBI57_001781"
     CDS             7935..>8046
                     /locus_tag="NBI57_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1822716.1"
                     /translation="MFYFTLSKQNTHSEKLNFSLIANINKIDTKLFERLLK"
BASE COUNT         2934 a         1206 c         1348 g         2558 t
ORIGIN      
        1 cattgatgtc tatagagtgg gacgagaaaa tatagttata gctgtctata acgagcatat
       61 taagctttta tttatactga tatcttgaat ttaattaata gaaacctata aaaaaacagt
      121 aagccattta aatgacttac tgttttttga attaggccaa caatattaac gtataccttt
      181 catcgctttg atgattaaag gtgagaatgc taatacaatt gttgtaacaa taattgcaac
      241 aacacctagg aaaataaagt aatttgtttg acctagtggt tctattaact taactaaagt
      301 accattgatt gcttgtgcag aagcgttagt taagtaccaa atactcatca tttgggcatt
      361 aaatgcttta ggtgctaact taacagcagc actattaccc gttggtgata agcatagctc
      421 accgataaca caaataatgt acgataaaat aacccagtta actgagaagt ttgatgaacc
      481 tgatgcataa cctacaatac caattagtat gtatgacgca cctgctaaga acgtaccaat
      541 tgcaaatttt actggcaggc taggttgttt agttccaagc ttttgccata aaagtgaaat
      601 aattggagct aataataaaa taaataatgg gttaattgat tggaagatcg cttcaccaaa
      661 gtttgttttc caaccaaata agtttaattt catatctgaa tgttcaattc catatatgtt
      721 taatacatta gacccttgtt cttgaatagc ccagaacacc attccaagaa taaataatgg
      781 aataaatgct ttaacacgag aacgttcagt atcagtgaca tctttacttc taataattaa
      841 agtgaagtaa atgattggta atgcaatacc taatactaaa acagtattac taactaagtt
      901 aaatgataat gagttagtta atgcaccaat aacgataatt aatacaattg ctaagacaac
      961 acttccgata ataagaccat actttttctt ttcagctggt gtcaatgggt tagtaggttt
     1021 cataccaacg ctacctaagt ttttgcggtt gaaaagtaca taccatacta aacctaatgc
     1081 cataccaact gctgcaatca agaatccgcc gtggaagttt ttaacattaa caaagtgttg
     1141 caaaataata ggtgataata atgcacccat attaactgac atatagaaaa taacaaaacc
     1201 tgcatccata cgtctatcat tttcaggata taaacggcca acgatatttg aaatgtttgg
     1261 cttcattaaa cctgaaccaa taatgatgaa gaacattgat gtgaataagc cgattaatgc
     1321 aaatggtaag cttaaacaaa tatgtccgat aataataaag actgcaccta ataaagtagc
     1381 gcctctagtg cctgtaattc tgtcagcaat ccatccgcct ggtattgatg tcatatagat
     1441 taatgaacca taaactgaca taattgacat agctgttgtt ttatcaattc caaggccatt
     1501 atctgttacg gcaaagtaca tgtagaaaat gagtagggca cgcatgccat aataactaaa
     1561 cctttcccag aactctacaa agaagagtac gcctagtcct cgaggatgcc cgaaaaatcc
     1621 tgtttgaggt atgtcttgaa tttgatttcc atgggagttt tgttgtgtca tgtatacatc
     1681 ccatcctttc ttcccctaat acaatagttt aagtaaattt gattgtgcct tatcaactaa
     1741 atattccaac aaatcacaaa aaaataaaaa tattgagaat attctgttat taagcgcgca
     1801 caattaatta gagataatat aaaaatatac ttattgacgt taaagtgcaa taggtttcta
     1861 taaaaatgtt gtttaattcg tgtactaata ttcttttggt gtactttggt ataaaatgtt
     1921 attttaatgt aacctattat gtatgctaaa tgaaaaacac accaataagt taggaaaatc
     1981 aacttattgg tgtgtttatt aaatgataca tttaacgatt atctattttt tcgggatata
     2041 aatcatgatt catcaaacga tgctcagcca ttttttcata tttagaattt ggacgtccat
     2101 agtttgtata aggatcaata gaaattccac cacgtggtgt gaacttgccc cagacttcaa
     2161 tataatgtgg gtccataagc tctatcaaat cattcataat aatattcata caatcttcgt
     2221 gaaaatcacc gtgatttctg aaactaaata agtataattt caaagatttt gattcaacca
     2281 ttttaacatt tggaatatat gaaatataga tagttgcaaa atctggttgc ccagtaattg
     2341 gacataatga tgtaaattct ggacagttga attttacgaa atagtcacga ccttgatgct
     2401 tattatcaaa cgattctaat acatcaggac gatagtcaaa attgtaagta ttgtcttgat
     2461 ttcctaataa agttatatct tgtaattcat cttgttgacg gccatgtgcc atataaagcg
     2521 ctcctttaaa tttatttttt tattattttg gcgtctcggc gtgctttttc aaacatgtaa
     2581 taacttgcac cgataataac gacgtaacct aatgttgcat agaaatctgg agattctccg
     2641 aatagaacaa atccaagtat tgctgtgaaa attatagatg catacgtaaa aatagaaata
     2701 tcttttgctg ctgcaaaact atatgctaaa gtaacaccaa tttgacccac agcggcagct
     2761 aagccagccc ctaatagata aagtatttgc atctgactca ttggttcata agtatatgca
     2821 gtgaaaggta ttaaaacgat gacagaaaat aaggagaagt aaaatactat agtatatggt
     2881 gcttctcttg aactaagtgc tcgaacacat gtatatgctg atgctgcaaa aatacctgag
     2941 aataagccag ctaatgatgg aatcatagat gatgaaaatt caggtttcac tattaaaagc
     3001 atacctaaaa tagcaattat cattgctgta atttgatact tccttacctt ttcatgtaag
     3061 aaaacaatgc ttaataaaat cgtccagaaa ggattgagtt tcattaatga atcggcatca
     3121 ctaagtacca tatgatcaat ggcataaata tttaacaata caccaataag tccaagtgtt
     3181 gatcgtgtta ttaataaggg ttgacttgaa agtctgccaa acattggctg atggtattta
     3241 tataaaaaaa ataatggaat aaacattgct actaagtttc gtgctaatga tttttgaaaa
     3301 acaggaaggt cacctgcaag tctgaaaaac actgacataa aactgaaacc aatagccgaa
     3361 attaaaatgg caatgatacc ttttacttta ggattcaatt ttatcgcctc ttttatataa
     3421 aattaacgta tttatattag cataaaacaa catgttgtgc ataaatagtt gaaatttact
     3481 ataaaaagac tataatagac tgtaccgaac aaacgttctg tgtttatttg tcggaataat
     3541 agggcattac acttttatga atgtttgtgt tattacataa aacaaatatc aattcagtat
     3601 caagctaata agctttttct tgatttctgt tgatacaatt gagattgaca cagatttaaa
     3661 aaaatcaagt gatatctact aaaaaatttt tttaaatttg ttcaagtttt tctaatttag
     3721 tattggtgcc tagttggaac gttttacgaa cattcgatta gaaaatggca ctttaaatca
     3781 tagtgtgtct tatgtataat gaaacacata atatagtgtt ggtgaaacga aaaagacaca
     3841 atatcttgtg ttttgtatgc aaatgcttta tttatgaaga aattacattt aaaagtaatt
     3901 taacacagaa atttaatagt tattatcaat taatagtcat atttttagaa aatgtactga
     3961 gcaaatggaa gatatccaat gatgtaaata ctacatatag tgatttttat acattcaacc
     4021 catataagct actattttct caaatataaa tctatgcaat tggtttacat ttgagaaaat
     4081 aagtagcttc attatagtta atacaatgct gagataacca tagtaaccat gttgttaaag
     4141 cattttttaa ttggaatgac tactttattt aaaagggttg aagaaagaag gtgatccaat
     4201 gaaaataata tatttttcat ttactggaaa tgtccgtcgt tttattaaga gaacagaact
     4261 tgaaaatacg cttgagatta cagcagaaaa ttgtatggaa ccagttcatg aaccgtttat
     4321 tatcgttact ggcactattg gatttggaga agtaccagaa cccgttcaat cttttttaga
     4381 agttaatcat caatacatca gaggtgtggc agctagcggt aatcgaaatt ggggactaaa
     4441 tttcgcaaaa gcgggtcgca cgatatcaga agagtataat gtccctttat taatgaagtt
     4501 tgagttacat ggaaaaaaca aagacgttat tgaatttaag aacaaggtgg gtaattttaa
     4561 tgaaaaccat ggaagagaaa aagtacaatc atattgaatt aaataatgag gtcactaaac
     4621 gaagagaaga tggattcttt agtttagaaa aagaccaaga agctttagta gcttatttag
     4681 aagaagtaaa agacaaaaca atcttcttcg atactgaaat cgagcgttta cgttatttag
     4741 tagacaacga tttttatttc aatgtgtttg atatttatag tgaagcggat ctaattgaaa
     4801 tcactgatta tgcaaaatca atctcgttta attttgcaag ttatatgtca gctagtaaat
     4861 ttttcaaaga ttacgctttg aaaacaaatg ataaaagtca atacttagaa gactataatc
     4921 aacacgttgc cattgttgct ttatacctag caaatggtaa taaagcacaa gctaaacaat
     4981 ttatttctgc tatggttgaa caaagatatc aaccagcgac accaacattt ttaaacgcag
     5041 gccgtgcgcg tcgtggtgag ctagtgtcat gtttcttatt agaagtggat gacagcttaa
     5101 attcaattaa ctttattgat tcaactgcaa aacaattaag taaaattggg ggcggcgttg
     5161 caattaactt atctaaattg cgtgcacgtg gtgaagcaat taaaggaatt aaaggcgtag
     5221 cgaaaggcgt tttacctatt gctaagtcac ttgaaggtgg ctttagctat gcagatcaac
     5281 ttggtcaacg ccctggtgct ggtgctgtgt acttaaatat cttccattat gatgtagaag
     5341 aatttttaga tactaaaaaa gtaaatgcgg atgaagattt acgtttatct acaatatcaa
     5401 ctggtttaat tgttccatct aaattcttcg atttagctaa agaaggtaag gacttttata
     5461 tgtttgcacc tcatacagtt aaagaagaat atggtgtgac attagacgat atcgatttag
     5521 aaaaatatta tgatgacatg gttgcaaacc caaatgttga gaaaaagaaa aagaatgcgc
     5581 gtgaaatgtt gaatttaatt gcgcaaacac aattacaatc aggttatcca tatttaatgt
     5641 ttaaagataa tgctaacaga gtgcatccga attcaaacat tggacaaatt aaaatgagta
     5701 acttatgtac ggaaattttc caactacaag aaacttcaat tattaatgac tatggtattg
     5761 aagacgaaat taaacgtgat atttcttgta acttgggctc attaaatatt gttaatgtaa
     5821 tggaaagcgg aaaattcaga gattcagttc actctggtat ggacgcatta actgttgtga
     5881 gtgatgtagc aaatattcaa aatgcaccag gagttagaaa agctaacagt gaattacatt
     5941 cagttggtct tggtgtgatg aatttacacg gttacctagc aaaaaataaa attggttatg
     6001 agtcagaaga agcaaaagat tttgcaaata tcttctttat gatgatgaat ttctactcaa
     6061 tcgaacgttc aatggaaatc gctaaagagc gtggtatcaa atatcaagac tttgaaaagt
     6121 ctgattatgc taatggcaaa tatttcgagt tctatacaac tcaagaattt gaacctcaat
     6181 tcgaaaaagt acgtgaatta ttcgatggta tggctattcc tacttctgag gattggaaga
     6241 aactacaaca agatgttgaa caatatggtt tatatcatgc atatagatta gcaattgctc
     6301 caacacaaag tatttcttat gttcaaaatg caacaagttc tgtaatgcca atcgttgacc
     6361 aaattgaacg tcgtacttat ggtaatgcgg aaacatttta ccctatgcca ttcttatcac
     6421 cacaaacaat gtggtactac aaatcagcat tcaatactga tcagatgaaa ttaatcgatt
     6481 taattgcgac aattcaaacg catattgacc aaggtatctc aacgatcctt tatgttaatt
     6541 ctgaaatttc tacacgtgag ttagcaagat tatatgtata tgcgcactat aaaggattaa
     6601 aatcacttta ctatactaga aataaattat taagtgtaga agaatgtaca agttgttcta
     6661 tctaacaatt aaatgttgaa aatgacaaac agctaatcat ctggtctgaa ttagcagatg
     6721 attagactgc tatgtctgta tttgtcaatt attgagtaac attacaggag gaaattatat
     6781 tcatgatagc tgttaattgg aacacacaag aagatatgac gaatatgttt tggagacaaa
     6841 atatatctca aatgtgggtt gaaacagaat ttaaagtatc aaaagacatt gcaagttgga
     6901 agactttatc tgaagctgaa caagacacat ttaaaaaagc attagctggt ttaacaggct
     6961 tagatacaca tcaagcagat gatggcatgc ctttagttat gctacatacg actgacttaa
     7021 ggaaaaaagc agtttattca tttatggcga tgatggagca aatacacgcg aaaagctatt
     7081 cacatatttt cacaacacta ttaccatcta gtgaaacaaa ctacctatta gatgaatggg
     7141 ttttagagga accccattta aaatataaat ctgataaaat tgttgctaat tatcacaaac
     7201 tttggggtaa agaagcttcg atatacgacc aatatatggc cagagttacg agtgtatttt
     7261 tagaaacatt cttattcttc tcaggtttct attatccact atatcttgct ggtcaaggga
     7321 aaatgacgac atcaggtgaa atcattcgta aaattctttt agatgaatct attcatggtg
     7381 tatttaccgg tttagatgca cagcatttac gaaatgaact atctgaaagt gagaaacaaa
     7441 aagcagatca agaaatgtat aaattgctaa atgacttgta tttaaatgaa gagtcataca
     7501 caaaaatgtt atacgatgat cttggaatca ctgaagatgt gctaaactat gttaaatata
     7561 atggaaacaa agcactttca aacttaggct ttgaacctta ttttgaggaa cgtgaattta
     7621 acccaatcat tgagaatgcc ttagatacaa caactaaaaa ccatgacttc ttctcagtaa
     7681 aaggtgatgg ttatgtatta gcattaaacg tagaagcatt acaagatgat gactttgtat
     7741 ttgacaacaa ataacaatta aattaaaaga ccttcacatg taaagggaaa tagcgattcg
     7801 tttcgtcttg tctcctacat gttgaaggtc ttttctctat gcttaaaagc gtatgcatat
     7861 tttgaaaaag taaaaattgt atttaatgat gtcaatgagg ctagaaagta tacaagatat
     7921 tgcattacaa aagggtgttc tattttactt taagcaaaca aaatacacat tccgaaaaat
     7981 taaatttcag tttaattgca aatatcaata aaattgacac taaattattt gaaaggctat
     8041 tgaaat
//
LOCUS       DAHHSC010000111         7837 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.112, whole genome shotgun sequence.
ACCESSION   DAHHSC010000111 DAHHSC010000000
VERSION     DAHHSC010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 7837)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7837)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7837
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.112"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            126..2222
                     /gene="pnp"
                     /locus_tag="NBI57_001782"
     CDS             126..2222
                     /gene="pnp"
                     /locus_tag="NBI57_001782"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HCD1822717.1"
                     /translation="MSQEKKVFKTEWAGRSLTIETGQLAKQANGAVLVRYGDTVVLST
                     ATASKEPRDGDFFPLTVNYEEKMYAAGKIPGGFKKREGRPGDDATLTARLIDRPIRPL
                     FPKGYKHDVQIMNMVLSADPDCSPQMAAMIGSSMALSVSDIPFQGPIAGVNVGYIDGK
                     YIINPTVEEKEVSRLDLEVAGHKDAVNMVEAGASEITEQEMLEAIFFGHEEIQRLVDF
                     QQQIVDHIQPVKQEFIPAERDEALVERVKSLTEEKGLKETVLTFDKQQRDENLDNLKE
                     EIVNEFIDEEDPENELLIKEVYAILNELVKEEVRRLIADEKIRPDGRKPDEIRPLDSE
                     VGILPRTHGSGLFTRGQTQALSVLTLGALGDYQLIDGLGPEEEKRFMHHYNFPNFSVG
                     ETGPVRAPGRREIGHGALGERALKYIIPDTADFPYTIRIVSEVLESNGSSSQASICGS
                     TLALMDAGVPIKAPVAGIAMGLVTREDSYTILTDIQGMEDALGDMDFKVAGTKEGITA
                     IQMDIKIDGLTREIIEEALEQARRGRLEIMNHMLQTIDQPRTELSAYAPKVVTMTIKP
                     DKIRDVIGPGGKKINEIIDETGVKLDIEQDGTIFIGAVDQAMINRAREIIEEITREAE
                     VGQTYQATVKRIEKYGAFVGLFPGKDALLHISQISKNRIEKVEDVLKIGDTIEVKITE
                     IDKQGRVNASHRALEE"
     gene            2458..4131
                     /locus_tag="NBI57_001783"
     CDS             2458..4131
                     /locus_tag="NBI57_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002455407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HCD1822718.1"
                     /translation="MSLIKKKNKDIRIIPLGGVGEIAKNMYIVEVDDEMFMLDAGLMF
                     PEDEMLGIDIVIPDISYVLENKDKLKGIFLTHGHEHAIGAVSYVLEQLDAPVYGSKLT
                     IALIKENMKARNIDKKVRYYTVNNDSIMRFKNVNISFFNTTHSIPDSLGVCIHTSYGA
                     IVYTGEFKFDQSLHGHYAPDIKRMAEIGEEGVFVLISDSTEAEKPGYNTPENVIEHHM
                     YDAFAKVRGRLIVSCYASNFIRIQQVLNIASKLNRKVSFLGRSLESSFNIARKMGYFD
                     IPKDLLIPITEVDNYPKNEVIIIATGMQGEPVEALSQMAQHKHKIMNIEEGDSVFLAI
                     TASANMEVIIANTLNELVRAGAHIIPNNKKIHASSHGCMEELKMMINIMKPEYFIPVQ
                     GEFKMQIAHAKLAAEAGVAPEKIFLVEKGDVINYNGKDMILNEKVNSGNILIDGIGIG
                     DVGNIVLRDRHLLAEDGIFIAVVTLDPKNRRIAAGPEIQSRGFVYVRESEDLLREAEE
                     KVREIVEAGLQEKRIEWSEIKQNMRDQISKLLFESTKRRPMIIPVISEI"
     gene            4388..6754
                     /locus_tag="NBI57_001784"
     CDS             4388..6754
                     /locus_tag="NBI57_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HCD1822719.1"
                     /translation="MAQAKKKSTAKKKTASKKRTNSRKKKNDNPIRYVIAILVVVLMV
                     LGVFQLGIIGRLIDSFFNYLFGYSRYLTYILVLLATGFITYSKRIPKTRRTAGSIVLQ
                     IALLFVSQLVFHFNSGIKAEREPVLSYVYQSYQHSHFPNFGGGVLGFYLLELSVPLIS
                     LFGVCIITILLLCSSVILLTNHQHRDVAKVALENIKAWFGSFNEKMSERNQEKQLKRE
                     EKARLKEEQKARQNEQPQIKDVSDFTEVPQERDIPIYGHTENESKSQCQPSRKKRVFD
                     AENSSNNIVNHQADQQEQLTEQTHNSVESENTIEEAGEVTNVSYVVPPLTLLNQPAKQ
                     KATSKAEVQRKGQVLENTLKDFGVNAKVTQIKIGPAVTQYEIQPAQGVKVSKIVNLHN
                     DIALALAAKDVRIEAPIPGRSAVGIEVPNEKISLVSLKEVLDEKFPSNNKLEVGLGRD
                     ISGDPITVPLNEMPHLLVAGSTGSGKSVCINGIITSILLNAKPHEVKLMLIDPKMVEL
                     NVYNGIPHLLIPVVTNPHKAAQALEKIVAEMERRYDLFQHSSTRNIKGYNELIRKQNQ
                     ELDEKQPELPYIVVIVDELADLMMVAGKEVENAIQRITQMARAAGIHLIVATQRPSVD
                     VITGIIKNNIPSRIAFAVSSQTDSRTIIGTGGAEKLLGKGDMLYVGNGDSSQTRIQGA
                     FLSDQEVQDVVNYVVEQQQANYVKEMEPDAPVDKSEMKSEDALYDEAYLFVVEQQKAS
                     TSLLQRQFRIGYNRASRLMDDLERNQVIGPQKGSKPRQVLIDLNNDEV"
     gene            6759..7472
                     /locus_tag="NBI57_001785"
     CDS             6759..7472
                     /locus_tag="NBI57_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCD1822720.1"
                     /translation="MSEMNAVYNVKQYILNLIKQNKLEYGDQLPSNLSIARELNVKTD
                     DVYEAIQALITEQVIKDNFEEGTSVKSLPPFFYPLNELISIGQMIKNAGFECGTEYLN
                     FDEQPATMLDANLLSVEEGYPVTIIERLRTADGEPVVYCLDKIAKKELTCTEYQMSNG
                     SILSAIKEQSNHNICYADTEIEAVNYEPRISEVLNASPHEGLILLKITHYNELDEPIL
                     YSLNYMKNSLVQFKITRKI"
     gene            7503..>7837
                     /locus_tag="NBI57_001786"
     CDS             7503..>7837
                     /locus_tag="NBI57_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HCD1822721.1"
                     /translation="MSRQSQPNIHIKVSPTTKFKTTTIVFKFMAPLEYDTITARSLLS
                     KLLVRATKKWPTDKSFNNHLADLYGAYVNSTISKFKDQHVITFSLEIVNERYLRNGES
                     LFNQGLDLL"
BASE COUNT         2842 a         1098 c         1540 g         2357 t
ORIGIN      
        1 ctattttgta aataatagat ggatatcccg ctctcttttt tccaattcaa tattttataa
       61 ctaatattaa aatacgataa taaatgatat gatataacta ttagattcaa gagaggagat
      121 ttataatgtc tcaagaaaag aaagttttta aaactgaatg ggcaggaaga tctttaacga
      181 ttgaaacagg gcaattagct aaacaagcaa atggcgctgt attggttcgt tatggagata
      241 cagtcgtgtt atcgacggca actgcatcaa aagaacctcg tgatggagat ttcttcccat
      301 taacagtgaa ctacgaagaa aaaatgtacg ctgcgggtaa aattcctggt ggatttaaaa
      361 agagagaagg acgtcctggt gacgatgcaa cattaactgc gcgattaatt gatagaccaa
      421 ttagaccttt attccctaaa gggtataagc atgatgttca aattatgaac atggtattaa
      481 gtgcagatcc tgattgttca ccacaaatgg ctgcaatgat tggttcatct atggcgctta
      541 gtgtgtcgga tattccattc caagggccaa tcgccggtgt aaatgtgggt tatattgatg
      601 gtaaatatat cattaaccca acagtagaag aaaaagaagt ttctcgttta gaccttgaag
      661 tagctggtca taaagatgca gtaaacatgg tagaggcagg cgctagtgag attactgaac
      721 aagaaatgtt agaggcgatt ttctttggtc atgaagagat tcaacgttta gttgatttcc
      781 aacaacaaat cgtcgaccac attcaacctg ttaaacaaga atttattcca gcagagcgtg
      841 atgaagcgct agttgaacgt gtgaaatctt taaccgaaga aaagggactt aaagaaacag
      901 ttttaacatt tgataaacaa caacgagatg aaaatcttga taacttaaaa gaagaaatcg
      961 tcaatgaatt tatcgatgaa gaagatccag agaatgaatt acttattaaa gaagtttatg
     1021 caattttaaa tgaattagtg aaagaagaag ttcgacgttt aattgcagat gaaaaaatta
     1081 gaccagacgg ccgtaaacct gatgaaatcc gtccattaga ttctgaagtt ggtattttac
     1141 ctagaacgca tggttcaggt ctatttacac gtggtcagac tcaagcactt tcagttttaa
     1201 cattaggtgc tttaggcgat tatcaattaa ttgatggttt aggacctgaa gaagaaaaaa
     1261 gattcatgca tcattataac ttcccgaact tttcagtagg tgaaactggt ccagtacgtg
     1321 cgccaggtcg tcgtgaaatt ggacatggtg cgttaggtga aagagcatta aaatatatta
     1381 ttcctgatac tgctgatttc ccatatacaa ttcgtattgt aagtgaggta cttgaatcaa
     1441 acggttcatc atctcaagcg tcaatttgtg gatcaacctt agcattaatg gatgcgggcg
     1501 taccgattaa agcaccagtt gctggtattg ctatgggcct tgttacacgt gaagatagct
     1561 atacgatttt aactgatatc caaggtatgg aagatgcatt aggtgatatg gactttaaag
     1621 tcgctggtac taaagaaggt attacagcaa tccaaatgga tattaaaatt gacggtttaa
     1681 cgcgtgaaat tatcgaagag gctctagaac aagcgagacg tggtcgttta gaaataatga
     1741 atcatatgtt acaaacaatt gatcaaccac gtactgaatt aagtgcttac gcgccaaaag
     1801 ttgtaactat gacaattaaa ccagataaga ttagagatgt tatcggacct ggtggtaaaa
     1861 aaattaacga aattattgat gaaacaggcg ttaaattaga tattgaacaa gatggtacta
     1921 tctttattgg ggccgttgat caagctatga taaatcgtgc tcgtgaaatc attgaggaaa
     1981 ttacacgtga agcggaagta ggtcaaactt atcaagccac tgttaaacgt attgaaaaat
     2041 acggtgcgtt tgtaggccta ttcccgggta aagatgcgtt gcttcacatt tcacaaattt
     2101 caaaaaatag aattgaaaaa gtggaagatg ttttaaaaat cggtgacaca attgaagtta
     2161 agattactga aattgataaa caaggtcgag taaatgcatc acacagagca ttagaagaat
     2221 aatatttaaa gtcatatgac gacaatgtat cgtcatgtga tttttttatg ccatttttta
     2281 cgaagtgatc cattttgaat ttgttgtatt gaacatttta aaacgcttta ttattttgag
     2341 tgcaactgtt aattatcctg tatgtatagt gattaatagt gtacatcaag tgttttttaa
     2401 cttataatga atagtgagtt tatatatgga cgggtaacaa atttaggagg taagattttg
     2461 agtttaataa agaaaaagaa taaagatatt cgcattatac cattaggcgg tgttggcgaa
     2521 attgctaaaa atatgtatat cgttgaagta gacgatgaaa tgtttatgtt agatgctgga
     2581 cttatgtttc cagaagacga aatgctaggt attgatattg ttataccaga catttcatac
     2641 gtacttgaaa ataaagataa attgaagggt atattcctta cacacggaca tgagcacgcg
     2701 attggtgcag tgagttatgt tttagaacaa ttagatgcac cagtatatgg atctaaattg
     2761 acaatagcgt taattaaaga aaatatgaaa gcccgtaata ttgataaaaa agttcgctac
     2821 tacacagtta acaatgattc aattatgaga ttcaaaaacg tgaatattag tttctttaat
     2881 acgacacaca gtattcctga tagtttaggt gtttgtattc acacttcata tggtgccatt
     2941 gtgtatacag gtgaatttaa gtttgaccaa agtttacatg gacattatgc accagatatt
     3001 aaacgtatgg cagagattgg tgaagaaggc gtatttgtct taatcagtga ttctactgag
     3061 gcagagaaac ctggatataa tactccggaa aatgtaattg aacatcatat gtatgatgct
     3121 tttgcaaaag tgcgaggtcg cttgattgtt tcatgttatg cttcgaactt tatacgtatt
     3181 cagcaagttt taaatattgc tagcaagcta aatcgtaaag tgtcattttt aggaagatca
     3241 cttgaaagtt catttaatat tgctcgtaaa atggggtatt tcgacattcc taaagatttg
     3301 ctaattccta taacagaagt tgataattat cctaaaaatg aagtgataat tatagctact
     3361 ggtatgcaag gagaacctgt agaagcctta agtcaaatgg cgcaacataa gcataaaatt
     3421 atgaatatcg aagaaggcga ttctgtattt ttagcaatta cggcttctgc taatatggaa
     3481 gttatcattg cgaatacatt aaatgagctt gtacgtgctg gcgcacatat tattccaaat
     3541 aacaagaaga ttcatgcttc aagtcatggt tgcatggaag aattaaaaat gatgattaat
     3601 attatgaaac ctgaatactt tattcctgta caaggtgaat ttaaaatgca gatagcacat
     3661 gcgaagctag cagctgaagc aggtgttgca ccggaaaaga ttttccttgt ggaaaaagga
     3721 gatgtcatta attacaacgg taaagatatg atattaaatg aaaaggtaaa ttcgggaaat
     3781 attttaatag atggtattgg tattggggat gtaggaaata tcgtgttgag agaccgtcat
     3841 cttttagcag aagatggtat ctttattgct gtagtaacgt tagatcctaa aaatagacgt
     3901 atagctgcgg gtcctgaaat tcaatctcgt gggtttgtat atgtacgtga aagtgaagac
     3961 ttattacgtg aagcagaaga gaaagtacgt gaaatagtag aagctggttt acaagaaaaa
     4021 cgcatagaat ggtctgaaat taagcaaaat atgcgtgatc aaattagtaa actattattc
     4081 gaaagtacaa aacgtcgtcc tatgattatt ccagtaattt ctgaaattta atcaaaaagt
     4141 cattaacata aaagaggtca gaacaagtca ccgaaatata atggttgtca tggacaattt
     4201 acttatattt tatgatggtc aattgaaagg ggtaacgatt aatctgttat cttaagtaaa
     4261 ttgatacata gatgatattg ttctaacctc tttcatcgtc tgtttggact acatattcta
     4321 aacatcaaat aggaaattat atataataac gtcgttttaa ctaaggcaac ataaggaggt
     4381 gcgtcaattg gcacaagcaa aaaagaaatc gacagctaag aaaaaaacag catcaaaaaa
     4441 aagaacaaat tcaaggaaaa agaagaatga taatccgata cgttatgtca tagctatttt
     4501 agtagttgta ttaatggtgt tgggtgtttt ccaattagga ataatcggtc gtctaattga
     4561 cagcttcttt aattatttat ttgggtacag tagatattta acatatattt tagtactctt
     4621 agcaactggt tttattacat actctaaacg tattcctaaa actagacgaa cggctggttc
     4681 gattgtattg caaattgcat tgctatttgt atcacagtta gtttttcatt ttaatagtgg
     4741 tatcaaagct gaaagagaac ctgtactttc ttatgtatat cagtcatacc aacacagtca
     4801 ttttccaaat tttggtggcg gtgtattagg tttttattta ttagagttaa gcgtaccttt
     4861 aatttcatta tttggtgtat gtattattac tattttatta ttatgctcaa gtgttatttt
     4921 attaacaaac catcaacatc gtgatgttgc aaaagttgca ctggaaaata taaaagcttg
     4981 gtttggttca tttaatgaaa aaatgtcgga aagaaaccaa gaaaaacaat tgaagcgtga
     5041 agaaaaagcg agacttaaag aagaacaaaa ggcacgtcaa aatgaacagc cacaaataaa
     5101 agatgtgagt gattttacgg aagtgcctca agaaagagat attccaattt atgggcatac
     5161 tgaaaatgaa agtaaaagcc agtgtcaacc aagtcgaaaa aaacgagtgt ttgatgcaga
     5221 gaatagttcg aataacatcg taaatcatca agcagatcag caagagcaat taacagaaca
     5281 aactcataac agtgttgaaa gtgaaaacac tattgaagaa gctggtgaag ttacgaatgt
     5341 atcgtatgtt gttccaccgt taactttact taatcaacct gcaaaacaaa aagcaacatc
     5401 taaagctgaa gtacaacgta aaggacaagt actagagaat acattaaaag attttggggt
     5461 aaatgcaaaa gtgacacaaa ttaaaattgg tcctgcagta actcaatatg aaattcaacc
     5521 agctcaaggg gttaaagtga gtaaaattgt aaacttgcat aatgatattg cattagcttt
     5581 agcagcaaaa gatgttagaa tcgaagcacc aatacctggt cgctctgcag taggtattga
     5641 agtgccaaat gagaaaattt cattagtttc actaaaagaa gttttagatg aaaaattccc
     5701 gtctaataat aaactagaag ttggattagg aagagatata tcaggtgatc caattactgt
     5761 tccactaaat gaaatgccac acttattggt ggcaggatcg acgggtagtg gtaaatctgt
     5821 ttgtataaat ggtattatta caagtatttt attaaatgct aagccgcatg aagttaaact
     5881 tatgttaatc gatccgaaaa tggttgaact aaatgtttat aacggaattc cacatttatt
     5941 aattccggtt gttacaaatc ctcataaagc tgctcaagct ttagaaaaaa ttgtagctga
     6001 gatggaaaga cgttatgatt tattccaaca ttcatcaact agaaatatta aaggttataa
     6061 cgaattaatc cgtaagcaaa atcaagaatt agatgagaag caaccagaat taccttatat
     6121 cgttgttatt gtagatgagc ttgcagattt aatgatggta gctggtaaag aagttgaaaa
     6181 tgcgattcaa cgtatcacac aaatggcacg tgcagcaggt atacatttga ttgtagcaac
     6241 acaaagacct tctgtggatg taattacagg tatcattaaa aataacattc catctagaat
     6301 tgcttttgct gtgagttctc aaacagattc aagaactatt attggtactg gcggcgcaga
     6361 aaagttactt ggtaaaggtg acatgttata cgttggaaat ggtgattcat cacaaacacg
     6421 tattcaaggg gcgtttttaa gtgaccaaga ggtgcaagat gttgtaaatt atgtagtaga
     6481 acaacaacag gcaaattatg taaaagaaat ggaaccagat gcaccagtgg ataaatcgga
     6541 aatgaaaagt gaagatgctt tatatgatga agcgtatttg tttgttgttg aacaacaaaa
     6601 ggcaagtaca tcattgttac aacgccaatt tagaattggt tataatagag catctaggtt
     6661 gatggatgat ttagaacgca atcaggtaat cggtccacaa aaaggaagca agcctagaca
     6721 agttttaata gatcttaata atgacgaggt gtaaaaaaat gtcagaaatg aatgcggtat
     6781 ataacgttaa acaatacatt ttaaatttga ttaagcaaaa taaattggaa tatggtgacc
     6841 aacttccaag taatttatca attgccagag aattaaatgt aaaaaccgac gatgtttatg
     6901 aagcaattca ggcattgatt actgaacaag tcattaaaga taattttgaa gagggcacaa
     6961 gtgttaaatc actgccccct ttcttttatc cattgaatga acttataagt attgggcaaa
     7021 tgattaaaaa tgcaggattt gaatgcggaa ctgaatactt aaattttgat gagcaaccag
     7081 caactatgtt agatgcaaat ttgttgagcg ttgaagaagg atatccagta accattatag
     7141 aacgattacg aactgccgat ggagaaccgg tcgtctattg tttagataaa attgctaaaa
     7201 aagaattaac atgtacagag tatcaaatga gcaatggatc gatactaagt gcgataaaag
     7261 aacaaagtaa tcataatatt tgttacgctg atacagaaat tgaagcggta aattatgaac
     7321 ctcgaatatc cgaagtactg aatgcttcgc cacacgaagg tttgatttta ttaaaaatta
     7381 cgcactacaa tgaattagat gaacctattt tgtattcatt aaattatatg aaaaatagct
     7441 tagttcaatt taaaatcact agaaaaatat agatgtaaga agatatagag gaggctatat
     7501 atttgagtag acaatctcaa ccaaatatac atatcaaagt ttcaccaaca actaaattta
     7561 aaacaactac tatagttttt aaatttatgg cacctttaga atatgacaca attacagcta
     7621 gatcattatt aagcaaatta ttagttcgag caactaagaa atggccaacc gataagtcgt
     7681 ttaataatca cttagccgat ttatatggtg cgtatgtgaa tagtacaatt tcaaaattca
     7741 aagatcagca tgtcattaca ttttcattag aaattgttaa tgaacgttat ttaagaaacg
     7801 gtgaatcatt atttaatcaa ggattagatt tattaca
//
LOCUS       DAHHSC010000112         7720 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.113, whole genome shotgun sequence.
ACCESSION   DAHHSC010000112 DAHHSC010000000
VERSION     DAHHSC010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 7720)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7720)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7720
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.113"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            complement(194..1021)
                     /locus_tag="NBI57_001787"
     CDS             complement(194..1021)
                     /locus_tag="NBI57_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCD1822722.1"
                     /translation="MWKWEAENDAKGVVVIAHNILEHTGRYAYVITMLRRNGYHVIMG
                     DLPGQGQTSRANKGQIENFQTYHESLLDWLKIANEYKIPTYVLGVGLGGLILLNLLEK
                     VELPIEGMMLISPMLELQKNGKNRKDKLVSNIGKISKDTRFNVGVEPKDLTRNLEIVE
                     ETVNDGLMLKKATYHWYNTINETMKDTMAHIHDIQPMPTLLMYGTKDLIVDTRAIDEF
                     KEKYQTPELYFKAWQGFYHEVHNEPERDEVMRYILTFLNNSVNTMGFIVEDDEIVEI"
     gene            complement(1024..1143)
                     /locus_tag="NBI57_001788"
     CDS             complement(1024..1143)
                     /locus_tag="NBI57_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1822723.1"
                     /translation="MMLISFIYNNMDNNNTINRNLHSEFAIIMLLKINEKGAV"
     gene            1255..2256
                     /locus_tag="NBI57_001789"
     CDS             1255..2256
                     /locus_tag="NBI57_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline dehydrogenase"
                     /protein_id="HCD1822724.1"
                     /translation="MALLKNFFIGLSNNSFLNNAAKKVGPRLGANKVVAGNTIPELIN
                     TIEYLNDKNIAVTVDNLGEFVGTVEESNHAKEQILTIMDALHQHGVKAHMSVKLSQLG
                     AEFDLELAYQNLREILLKANTYNNMHINIDTEKYASLQQIVQVLDRLKGEFRNVGTVI
                     QAYLYDSHKLVDKYQDLRLRLVKGAYKENESIAFQSKEDVDANYIKIIEQRLLNARNF
                     TSIATHDHRIINHVKQFMKENHIEKDRMEFQMLYGFRSELAEEIANEGYNFTIYVPYG
                     DDWFAYFMRRLAERPQNLSLAVKEFVKPAGLKRVGIIAALGATVMLGLSTIKKLCRK"
     gene            complement(2378..2842)
                     /locus_tag="NBI57_001790"
     CDS             complement(2378..2842)
                     /locus_tag="NBI57_001790"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HCD1822725.1"
                     /translation="MNFEGKLIGKDLKVAIVVSRFNDFITGRLLEGAKDTLIRHDVNE
                     DNIDVAFVPGAFEIPLVAKKLASSGNYDAIITLGCVIRGATSHYDYVCNEVAKGVSKV
                     NDQTNVPVIFGILTTESIEQAVERAGTKAGNKGAEAAVSAIEMANLLKSIKA"
     gene            complement(2855..4036)
                     /gene="ribB"
                     /locus_tag="NBI57_001791"
     CDS             complement(2855..4036)
                     /gene="ribB"
                     /locus_tag="NBI57_001791"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HCD1822726.1"
                     /translation="MQFDNIDSALMALKNGETIIVVDDENRENEGDLVAVTEWMNDNT
                     INFMAKEARGLICAPVSKDIAQRLDLVQMVDDNSDIFGTQFTVSIDHVDTTTGISAYE
                     RTLTAKKLIDPSSEAKDFNRPGHLFPLVAQDKGVLARNGHTEAAVDLAKLTGAKPAGV
                     ICEIMNDDGTMAKGQDLQNFKEKHQLKMITIDDLIEYRKKLEPEIEFKAKVKMPTDFG
                     TFDMYGFKATYTDEEIVVLTKGAIRQHENVRLHSACLTGDIFHSQRCDCGAQLESSMK
                     YINEHGGMIIYLPQEGRGIGLLNKLRAYELIEQGYDTVTANLALGFDEDLRDYHIAAQ
                     ILKYFNIEHINLLSNNPSKFEGLKQYGIDIAERIEVIVPETVHNHDYMVTKKIKMGHL
                     I"
     gene            complement(4047..4679)
                     /locus_tag="NBI57_001792"
     CDS             complement(4047..4679)
                     /locus_tag="NBI57_001792"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HCD1822727.1"
                     /translation="MFTGIVEEIGVVKSVQIRQSVRTIEIEAHKITADMHIGDSISVN
                     GACLTVIDFDQTSFTVQVIKSTENKTYLADVKRQSEVNLERAMSGNGRFGGHFVLGHV
                     DELGTVSKINETANAKIITIQCSQHINNQLVKQGSITVDGVSLTVFDKHDNSFDIHLI
                     PETRRSTILSSKKLGDKVHLETDVLFKYVENILNKDKDQLSVDKLRAFGF"
     gene            complement(4686..5729)
                     /gene="ribD"
                     /locus_tag="NBI57_001793"
     CDS             complement(4686..5729)
                     /gene="ribD"
                     /locus_tag="NBI57_001793"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015581873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HCD1822728.1"
                     /translation="MSQFMDYAIQLANMVQGQTGVNPPVGAVVVNEGRIVGIGAHLRK
                     GDKHAEVQALDMAQQNAEGATIYITLEPCSHFGSTPPCVNKIIDCKIAKVVYATKDNS
                     LDTHGDETLRAHGIEVECVDDERASQLYQDFFKAKAKQLPQITVKVSASLDGKQANDN
                     GQSQWITNKEVKQDVYKLRHRHDAVLTGRRTVELDDPQYTTRIQDGKNPIKVILSKSG
                     NIHFNQQIYQDESTPIWIYTENPNLTSNQTHIEIIYLKSCDLTTILHNLYKRGVGTLL
                     VEAGPTTTSEFLQSNYIDEFILYYAPKLIGGSGNYQFYQTNDVIEIPDANQFEIVHSE
                     LLNQNVKLTLRKK"
     regulatory      complement(5856..5991)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(6210..7712)
                     /locus_tag="NBI57_001794"
     CDS             complement(6210..7712)
                     /locus_tag="NBI57_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HCD1822729.1"
                     /translation="MRVAIIGMGTAGVSVLRELVKHPKFNQLDIDLYDDKVNMGQGVP
                     FQNDSSELLINMPSKKMSLNLDDETEFWKWYKQQTDFNFDEPAYLPRFVFGHYMKSYL
                     SMFTKKYPNISTNYNKVQEIYTNSNIDETNLTYYICTTNSEQSWQAYDYVFLTCGTFA
                     YHDPYNLKGKKGYIATPYPTYNTLDEVNELDDIAIIGTGLASLDVVRYVAAHHPKLPI
                     TMTSRSAHLPSVRGTMIDVTFKYLTKDKLNDIKKHHFGNAPLDTIVSLFLKECAEYDI
                     DFKKLVHRRTGNHIADLKYDLARPTEMGIFQSMIEHLKENLNWIWNSLSIEDQHQFNQ
                     KYSKMIQLNSNPMPPRTAELIIELIENKSLILKKDLEDVKHDGKLYYFSYKNQESVDM
                     YNVVINATGAKSHLNELDQDDQLIKNLENRQIVQAHPMGGIQIIPETNQVISPRFGTL
                     TNMIAIGQMTNGVNKLRNGVKMIVEQVAHTVSQLYDALESNEQQQRSYNQ"
BASE COUNT         2455 a         1423 c         1078 g         2764 t
ORIGIN      
        1 attaattagt tagccttaaa tttaacaaat taaatcaaac cgaatgaggc taaattggtt
       61 atttttcaag atatgatttg tataaatcaa aacatttctt acaaatcaaa tgtgtataaa
      121 atgtgatata cattctcaac cttaaaaaag taaaaaaaaa gatgagcatc tactgtctca
      181 tctttttaga atattaaatt tctacaattt cgtcatcttc aacaataaag cccattgtat
      241 tgacgctgtt atttaagaaa gtcagaatat aacgcattac ttcatcacgt tctggctcat
      301 tgtgaacctc gtggtaaaaa ccttgccaag ctttaaaata taattcaggt gtttgatatt
      361 tttctttaaa ctcatcaatt gccctagtat caacaattaa atccttcgtt ccatacatta
      421 atagcgttgg cattggttga atgtcatgaa tatgagccat cgtatctttc atcgtctcat
      481 taattgtatt ataccaatga tacgttgctt tttttaacat taaaccatcg ttaactgttt
      541 cttcaacaat ttctaaatta cgtgttaaat cttttggttc tacaccaaca ttaaaacgtg
      601 tgtcttttga aattttacct atatttgaaa caagtttatc tttacgattt tttccattct
      661 tttgaagttc tagcatagga gaaattaaca tcatcccctc gattggcaat tctacttttt
      721 caagtaaatt taataaaatc aaaccgccaa gtcctacccc taatacataa gtaggaattt
      781 tatattcatt agctatcttt aaccaatcta gcaaactttc gtgatacgtt tgaaagtttt
      841 caatttgtcc tttattagct cttgaagttt gaccttgacc aggcaaatct cccataatca
      901 catgatagcc atttcttctt aacatcgtaa taacatatgc atatcttccc gtatgttcta
      961 atatattatg agcaataaca acgacgcctt tcgcatcatt ttcagcttcc cacttccaca
     1021 ttattatact gccccttttt cattaatctt caataacata attatagcaa attcactatg
     1081 tagatttcta tttatagtat tattgttgtc catattatta tatataaatg aaatcaacat
     1141 caataatagt gtaattatac ataattattt ttgattgttt tttatgaaaa cgctttctcg
     1201 aatatttttt tcatgctaaa cttattgtaa acacaagggt ttggaggagt agcaatggca
     1261 ctattaaaga atttttttat cggattatct aataatagtt ttttaaacaa cgcagcaaaa
     1321 aaagtgggcc cacgtttggg cgccaataaa gtcgttgccg gaaatacaat tccagagtta
     1381 attaatacaa tcgaatactt aaatgacaag aatatcgctg ttacggtaga caatttaggg
     1441 gaatttgtcg gtacagttga agaaagtaat catgctaaag aacaaatttt aacaattatg
     1501 gacgcgcttc atcaacatgg cgtaaaggca catatgtctg ttaaattgag tcagttaggt
     1561 gcagaattcg acttagaatt agcttaccaa aatttaagag aaattttact taaagcaaat
     1621 acttacaaca atatgcatat aaatattgat actgaaaaat atgctagcct gcaacaaatt
     1681 gttcaagttt tagatcgctt aaaaggcgaa tttagaaatg ttggtactgt aattcaagca
     1741 tatttatacg atagccacaa attagttgat aagtaccaag atttacgatt acgtttggtt
     1801 aaaggtgcat ataaagaaaa cgaatcaatt gcatttcaat ctaaggaaga cgtagatgca
     1861 aattacatca aaataattga acaacgtttg ttaaacgcac gcaatttcac ttcaattgca
     1921 acacatgacc atcgcatcat taatcatgta aaacaattta tgaaagaaaa tcacattgaa
     1981 aaagatcgta tggaattcca aatgctctat ggttttagat cagagttagc agaagaaatc
     2041 gcaaatgaag gctataattt cactatttat gtaccttatg gcgatgattg gtttgcgtat
     2101 tttatgagaa gattagcaga acgcccacaa aacctatctc ttgctgtaaa agaatttgtg
     2161 aaacctgctg gcttaaaacg tgttggcata attgcagctt taggagctac agttatgtta
     2221 ggtttaagta caattaaaaa attatgccgt aaatagagca tggcataaac aataatttag
     2281 gagtctggaa caataatcaa tgttctaggc tcctaaatgt tatattggca gttctgaaac
     2341 atttataaat ctttgattat gaactcacaa cagcgctcta tgcttttata gattttaata
     2401 aattagccat ttcaattgca cttactgctg cttcggcacc tttattacca gctttcgtac
     2461 ctgctctttc cacagcttgt tcaatacttt cagtcgttaa aataccaaat atgactggta
     2521 cattagtttg atcatttact ttagaaacac ctttcgcgac ttcattacaa acataatcat
     2581 aatgagacgt agcaccgcga attacgcatc ctaatgtaat tattgcatca taatttcctg
     2641 atgaggctaa ttttttagct actaaaggaa tttcaaacgc accaggaaca aatgctacat
     2701 caatattgtc ttcattaaca tcatgtcgaa tcaaagtatc ttttgcacct tcaagtaatc
     2761 ttccagtgat aaaatcatta aatcgactaa ctacgattgc aactttcaaa tcttttccaa
     2821 ttaatttacc ttcaaaattc atgttaaagt cctcctatat taaatgaccc atttttattt
     2881 ttttcgttac catataatca tgattatgta ccgtttctgg tacgataact tcaattcttt
     2941 ctgcaatatc aatgccatat tgttttaatc cctcaaattt acttggatta ttacttaata
     3001 aattgatatg ttcgatgtta aaatatttta aaatctgtgc agcaatatga taatctcgta
     3061 aatcttcatc aaaacctaat gctaaatttg cagttactgt atcatatcct tgctcaatta
     3121 attcatatgc gcgtaatttg tttaacaatc ctatgccacg accttcttga ggtagataaa
     3181 taatcatgcc accatgttca ttgatatact tcatagacga ttcaagttga gcaccacaat
     3241 cacaacgttg actatggaaa atatcgcctg taaggcacgc agaatgtaag cgtacatttt
     3301 catgttgtcg aattgcacct tttgtcagta caactatctc ttcatctgtg tatgtcgctt
     3361 taaaaccata catatcaaat gttccgaaat ctgtaggcat tttcactttt gccttaaatt
     3421 caatttctgg ttctaatttt ttacgatatt caattaaatc atcaatcgta atcatcttta
     3481 attgatgttt ttctttaaaa ttttgtaaat cttgtccttt cgccatcgtg ccgtcatcat
     3541 tcataatctc acaaatgaca ccagcgggct tggcaccagt aagtttagct aaatcaacag
     3601 ccgcttctgt gtgtccattt ctagctaata cgcctttatc ttgtgctact aatggaaata
     3661 aatgaccagg acgattaaaa tctttagctt cactactagg atcaatgagc tttttggcag
     3721 tcaatgtacg ttcataagca ctaattcctg ttgttgtatc tacatgatca atactcactg
     3781 taaattgcgt accaaagatg tcggagttat catcaaccat ttgtaccaaa tccaaacgtt
     3841 gtgcaatatc tttagacact ggtgcgcata ttaatcccct tgcttctttc gccataaaat
     3901 taatggtatt atcgttcatc cattcagtaa ccgctactaa atcaccttca ttttcacgat
     3961 tctcatcatc tactacaata attgtttctc cattttttaa agccattaaa gcactgtcaa
     4021 tattatcgaa ttgcatgcta cccctcctaa aaaccaaatg ctcttaattt atctacagat
     4081 aattggtctt tatctttatt taaaatattt tcaacatatt taaacaaaac gtctgtttct
     4141 aaatgtactt tatctcctaa ttttttggat gataaaatcg ttgaacgcct cgtttctgga
     4201 ataagatgaa tgtcaaaact gttatcatgc ttatcaaata ccgttagact tacaccatcc
     4261 acagtaatag acccttgctt aactaactga ttattaatat gttggctaca ttgaatcgta
     4321 ataattttgg cattggctgt ttcatttatt tttgaaactg ttcctagttc atctacatga
     4381 ccgaggacaa aatgtccacc aaacctaccg ttaccactca tggcacgctc taaatttact
     4441 tctgattgtc gcttaacatc tgctaaatag gttttatttt cagtgctttt aattacttga
     4501 acagtaaaag atgtctgatc aaaatcaatc actgttaaac atgcaccatt aacactgatg
     4561 gaatcaccaa tatgcatatc tgccgtaatc ttatgtgctt cgatttcaat cgtcctgact
     4621 gattgacgaa tttgaacact tttaacgaca cctatttctt caacgatgcc agtaaacatg
     4681 catcatcact tctttcgtaa agttaattta acattttgat ttaataactc ggaatgaaca
     4741 atttcaaatt ggttcgcatc tggtatctca atcacatcat ttgtttgata aaattgataa
     4801 tttccagatc cgccaattaa tttcggggca taatagagaa taaattcatc tatataatta
     4861 gattggagaa attctgaagt agtggttgga cctgcctcga ctagcaaagt tccaactcct
     4921 cttttatata aattgtgaag aattgttgtt aaatcacaag acttcaagta aataatttca
     4981 atatgtgttt gattgcttgt taaatttgga ttttcagtat atatccaaat tggtgttgat
     5041 tcatcttgat aaatttgctg attaaaatga atattcccag acttagacaa tattactttt
     5101 atagggtttt ttccatcttg aatacgtgta gtatattgtg gatcatctaa ttcaactgta
     5161 cgtcttccag ttaacactgc gtcgtgtcga tgtcttaact tatagacatc ttgtttaacc
     5221 tctttgttag taatccattg actttgtcca ttatcattcg cttgtttacc atctaaactt
     5281 gcagatactt tcactgtaat ttgtggcagt tgctttgctt ttgctttaaa aaagtcttgg
     5341 tataattgtg atgcccgttc atcatcaacg cattcaacct caataccgtg agcccgtaac
     5401 gtctcatcac catgtgtgtc taacgaattg tcttttgttg cgtatactac ttttgctatc
     5461 ttacaatcaa taattttgtt aacacagggt ggtgttgaac caaaatgact acatggctct
     5521 aacgtaatat aaatcgtcgc accttcagca ttttgttgtg ccatatcaag tgcttgaacc
     5581 tccgcatgct tgtcaccttt tctcaagtgt gcaccaatac caacaatcct accttcatta
     5641 actacaacag cgccaacggg tggattaaca cctgtttgac cttgtaccat atttgcaagt
     5701 tgaatcgcat aatccataaa ttgactcaaa tgatcacctc tataaacaaa aatcctcaca
     5761 tcatgaatta agatgcaagg agaaaaattt atcgttaaat aagcctattt gtacacattt
     5821 ttacaaatac gctacattat ctttgtcgat aattaacatt ctttctccca tccagacttt
     5881 aactgtcggc tctagaatct cactagatca gccactaata tgaaacatat tagcaggtcg
     5941 caggctttat ttactgccgg ttgggaattt caccctgccc cgaaagaatt atatatgaaa
     6001 ttgttataga ttatttgagt acgtagtatg tcaactacat ttaaaatgat actatatgtt
     6061 ttctgaaaaa acaattaatg acggttttaa tttaatataa tctgagtact ataggcatct
     6121 cattgatatg attcttacta acagacatta aaatcaaacc ttcaattcgt ctctatagag
     6181 cgttctcttt attatcttct agttacaaat tattgattgt aactgcgctg ttgttgctca
     6241 ttcgattcta aagcatcata taattgagat actgtatgcg caacttgttc tactatcatt
     6301 ttcacaccat ttcgtagttt attaacaccg tttgtcattt gacctatcgc aatcatattt
     6361 gttaatgttc caaaccttgg actaataact tgattggttt ccggtatgat ttgtatgcct
     6421 cccattggat gtgcttgtac aatttgtcta ttttcaagat ttttaattaa ttgatcatct
     6481 tgatccaatt catttaaatg actttttgca cctgtcgcgt taatgacaac attatacatg
     6541 tctactgatt cttggttttt gtatgaaaaa taatacaact tgccatcatg tttcacatct
     6601 tctaaatctt ttttcaaaat taaagactta ttttctatta attcaataat tagttcagca
     6661 gttcttggag gcattggatt tgaatttaat tgaatcatct ttgagtattt ttgattaaat
     6721 tgatgttgat cttcaatact taagctattc catatccaat ttaaattctc tttcaaatgt
     6781 tcaatcatac tttggaaaat gcccatttct gttggacgcg ctaaatcata cttcaaatct
     6841 gcaatatgat ttcctgtacg tctatgtact aattttttaa aatcaatgtc atattcagca
     6901 cattctttta aaaataaaga aactatagta tcaagcggtg cattgccgaa atgatgtttt
     6961 ttaatgtcat ttaatttgtc tttagttaag tacttgaatg tcacgtctat cattgtacct
     7021 cttacacttg gtaaatgagc agaacgactc gtcatagtaa ttggtaattt cggatgatga
     7081 gcagcaacat aacggacaac atctaaactg gcaaggcctg taccaataat cgcaatatcg
     7141 tccagttcat ttacttcgtc taacgtatta tatgttggat aaggcgtagc gatatatcct
     7201 tttttaccct ttaagttata tggatcatgg taggcaaatg taccacatgt taaaaataca
     7261 taatcgtacg cttgccatga ttgttctgaa tttgtagtac atatgtaata agttaaattc
     7321 gtttcatcga tattagaatt tgtataaatc tcttgaactt tattataatt agttgatata
     7381 tttggatatt ttttcgtgaa catagataaa taagatttca tataatgtcc gaatacaaat
     7441 ctcggtaaat atgcaggttc atcaaaatta aaatcagttt gttgtttata ccacttccaa
     7501 aattcagtct catcatctaa atttaaactc atctttttcg aaggcatatt aattagcagc
     7561 tcagaactat cattttgaaa tggtacgccc tgtcccatat ttactttatc atcgtataaa
     7621 tctatatcta attgattaaa cttcgggtgc ttaactaact ctctcaatac acttacacca
     7681 gcagttccca tgcctattat tgctacacgc atatatcatc
//
LOCUS       DAHHSC010000113         7638 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.114, whole genome shotgun sequence.
ACCESSION   DAHHSC010000113 DAHHSC010000000
VERSION     DAHHSC010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 7638)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7638)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7638
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.114"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            complement(59..376)
                     /locus_tag="NBI57_001795"
     CDS             complement(59..376)
                     /locus_tag="NBI57_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HCD1822730.1"
                     /translation="MKSMVEMQREVDEYIGQFKTGYFSPLANLARLTEEVGELAREIN
                     HTYGEKKKKDSEEANTIKAELGDNLFVLLCLANSMGIDMTESFNETMEKFNTRDKNRF
                     ERK"
     gene            complement(712..1392)
                     /locus_tag="NBI57_001796"
     CDS             complement(712..1392)
                     /locus_tag="NBI57_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002456509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HCD1822731.1"
                     /translation="MIIYFVILMVLPLWAQHKVKSNYEKYSQVRSTSGKTGREVALEI
                     LHANGIYDVEVVKGEGFLTDHYDPKKKVVSLSPANYDRPSVAGTAIAAHEVGHAIQDH
                     QGYWFLRFRAALVPVANLGSSLSYMIIMLGIILTAIGSAFGSTALWIGAGLMSLAVLF
                     SIVTLPVEFDASSRAMKQITALNIVNEKEYKHARKVLSAAAMTYVAATAVAVAELVRI
                     ILIARSSD"
     gene            complement(1464..2051)
                     /locus_tag="NBI57_001797"
     CDS             complement(1464..2051)
                     /locus_tag="NBI57_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1405 domain-containing protein"
                     /protein_id="HCD1822732.1"
                     /translation="MTINTFWQYTLYQRSWLMMLLICNILGMIYGYIWYGEQLSHTPW
                     HFKIFVPDSPTAILYLVISISLILIQKQNSIIEALAFVTLFKYGIWAVIMNILFIIEQ
                     GDITVNGLVLMFSHSIMAVQAIYFYPRFKRSMIGISVAMIWVFLNDYIDYFHLQFPYY
                     DFITTHVWQIGVLSCCLSVFGLLLYIELNKLLKCK"
     gene            complement(2041..2616)
                     /locus_tag="NBI57_001798"
     CDS             complement(2041..2616)
                     /locus_tag="NBI57_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpiB family protein"
                     /protein_id="HCD1822733.1"
                     /translation="MSETLNQIKESFIEYLLFQYRFKSRIAVWVLNYIKVNEAKLANI
                     HFVDTKINHHETLEIAEVGSHASAIQFTKRNIKLMNTNEIFDYIANHNCAFDIQIHFA
                     NVSKREQRLDDLIVAQLTESPSYQTYLHDLNSMAIDRHKHALLIDYLLHNIDLSLQMN
                     EKQRFYQLTQILNTLKLVNKHNQFEDLADDD"
     gene            complement(2630..3874)
                     /locus_tag="NBI57_001799"
     CDS             complement(2630..3874)
                     /locus_tag="NBI57_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000389522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1822734.1"
                     /translation="MEDIYKLIDDINLQKLENLDSRVNEAITTDNDDALFILGETLYN
                     FGLMPQGLEVFRVLYHKYPDESELLIYFIEGLMSENQTDEALEYLSYVEPSPEKLMLE
                     ADLYQQINMMEVAIDKLQEALELEPNDPIIHFALAEMLYYDGQYLRATSEYETVLETG
                     EYQVNGVNLFSRMADCSLQSGNYSDAIRLYDEINEDEMTSEDYLKKAISYDKNDITQE
                     AIKIMTTLLSKDPDYIQGYLYLQSLYENEKNYPDAIETGKEGLRLSQFYKELMYTTGC
                     LEIEHGDANEGVLLLKQALEVDNAYQESLLILSDLYRNEEDYEAIIELLTYVDEEDLD
                     PTFMWHLAFAYGQEERDKEAQHFFELAYPTMKTNIDFMSDYYFYLIEIGQKEQAITIL
                     NQLLELEPSNENWHDELSRLQS"
     gene            complement(3881..5179)
                     /gene="aroA"
                     /locus_tag="NBI57_001800"
     CDS             complement(3881..5179)
                     /gene="aroA"
                     /locus_tag="NBI57_001800"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HCD1822735.1"
                     /translation="MVSEQIIDISGPLKGEIEVPGDKSMTHRAIMLASLAEGTSNIYK
                     PLLGEDCRRTMDIFRLLGVDIKEDEDKLVVNSPGYKAFKTPHQVLYTGNSGTTTRLLA
                     GLLSGLGIESVLSGDVSIGKRPMDRVLRPLKLMDANIEGIEDNYTPLIIKPSVIKGIN
                     YQMEVASAQVKSAILFASLFSNDTTVIKELDVSRNHTETMFRHFNIPIEAERLSITTT
                     PDAIQHIKPADFHVPGDISSAAFFIVAALITPESDVTIHNVGINPTRSGIIDIVEKMG
                     GNIQLFNQTTGAEPTASIRIQYTPMLQPITIEGELVPKAIDELPVIALLCTQAVGTST
                     IKDAEELKVKETNRIDTTADMLNLLGFELQPTNDGLIIHPSEFKTNATVDSLTDHRIG
                     MMLAVASLLSSEPVKIKQFDAVNVSFPGFLPKLKLLENEG"
     gene            complement(5189..6253)
                     /locus_tag="NBI57_001801"
     CDS             complement(5189..6253)
                     /locus_tag="NBI57_001801"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HCD1822736.1"
                     /translation="MKLQTTYPSNNYPIFVEHGAIDHISTYIDQFDQSFILIDEHVNQ
                     YFADKFDDILSYENVHKVIIPAGEKTKTFEQYQETLEYILSHHVTRNTAIIAVGGGAT
                     GDFAGFVAATLLRGVHFIQVPTTILAHDSSVGGKVGINSKQGKNLIGAFYRPTAVIYD
                     LDFLKTLPFEQILSGYAEVYKHALLNGESTTQEIEQHFKDREILQSLNGMDKYIAKGI
                     ETKLDIVVADEKEQGVRKFLNLGHTFGHAVEYNHKIAHGHAVMIGIIYQFIVANILFN
                     SNHDIQHYINYLTKLGYPLETITDIDFETIYQYMLSDKKNDKQGVQMVLIKHFGDIVV
                     QHIDQTTLQHACEQLKTYFK"
     gene            complement(6279..7445)
                     /gene="aroC"
                     /locus_tag="NBI57_001802"
     CDS             complement(6279..7445)
                     /gene="aroC"
                     /locus_tag="NBI57_001802"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HCD1822737.1"
                     /translation="MRYLTSGESHGPQLTVIVEGIPANLEIKVEDINKEMFKRQGGYG
                     RGRRMQIEKDTVEIVSGVRNGYTLGSPITMVVTNDDFTHWRKIMGAAPISEEERENMK
                     RTITKPRPGHADLVGGMKYNHRDLRNVLERSSARETAARVAVGALCKVLLQQLDIDIY
                     SRVVEIGGIKDKDFYDSETFKANLDRNDVRVIDDSIAQAMRDKIDEAKNEGDSIGGVV
                     QVVVENMPVGVGSYVHYDRKLDGKIAQGVVSINAFKGVSFGEGFKAAEKPGSEIQDEI
                     LYNSEIGYYRGSNHLGGLEGGMSNGMPIIVNGVMKPIPTLYKPLNSVDINTKEDFKAT
                     IERSDSCAVPAASIVCEHVVAFEIAKALLEEFQSNHIEQLQQQIADRRQLNVEF"
BASE COUNT         2450 a         1431 c         1034 g         2723 t
ORIGIN      
        1 ttcattggtc tatgttttta atcttaatca aatccaaata aagcatatat ttttaaattc
       61 actttctttc gaatcgattt ttatctcttg tattaaactt ttccattgtt tcattaaagc
      121 tctctgtcat atctattccc attgaattcg ctaaacataa caacacaaat aaattatcac
      181 ctaattctgc tttaatcgta tttgcttcct ctgaatcttt cttctttttt tcaccatagg
      241 tatgatttat ttcacgtgca agttcgccca cttcttcagt caatctagct aagttagcta
      301 atggtgaaaa atatcctgtt ttaaattgtc caatatattc atcaacttca cgttgcattt
      361 ctaccattga tttcatttct acgttctcct tatattgcat ttctaatata gtatatatca
      421 atttgaagtc tcatgcatgt ttagtgcaat ctactgactc tagctaaact agatgacaag
      481 ttgtttattt tagaagttta ataattcagt ttatataaat gtaatgcatt cctaactaaa
      541 ttaaatcaat tgaaattggg attataactt tatgattcgt accactacaa taaaataata
      601 taatgaataa tctaccatta gaaaaataag cacaaaaaaa ctagcaatca cacaaaatgt
      661 gattagctag ttaataagtg tctaatttaa gttaattgtt aatctataag attaatcact
      721 tgaacgcgca atcaaaataa tacgtacaag ctctgctaca gcgacagcag ttgctgcaac
      781 ataagtcatt gctgctgcag ataatacttt acgcgcatgc ttgtattctt tttcatttac
      841 aatgttcaat gccgtaattt gtttcatcgc tcttgaactc gcatcaaact caactggtaa
      901 cgtaacaatt gagaataata ccgctaatga cattaaacca gcaccaatcc ataaagcagt
      961 tgaaccaaat gcactaccta tcgctgttaa gataatacct aacatgatga tcatataact
     1021 taatgaactc cctaggtttg caacaggtac taatgctgct ctgaatctta agaaccaata
     1081 tccttggtga tcttgaatgg catgaccaac ttcgtgggct gcaattgcag ttccagcaac
     1141 tgatggtctg tcatagtttg caggagatag tgaaacaact ttctttttag gatcgtaatg
     1201 atctgttaag aatccttcac ctttaacaac ttcgacatca taaataccgt ttgcatgtaa
     1261 aatttctaat gcaacttcac gacccgtttt accactagtt gatctaactt gtgaatattt
     1321 ctcatagtta gatttaactt tgtgttgtgc ccataaagga agcaccatta atattacgaa
     1381 ataaattatc atagtaaaaa ttgaagacaa taaactcact ctcctttata aatattttac
     1441 tgtcatttgc cgtttttatc aaatcattta cactttaata atttgtttaa ttcaatataa
     1501 agcaaaagtc caaaaacact tagacaacat gataatacac caatttgcca cacatgtgta
     1561 gttataaaat cataatatgg aaattgaagg tgaaaatagt caatataatc attcaaaaac
     1621 acccaaatca tcgctacact gattccaatc atagaacgtt taaacctagg atagaagtaa
     1681 attgcctgaa cagccattat actgtgggaa aacattaata ccaaaccatt tactgtaata
     1741 tcaccttgtt caataataaa taatatattc attataactg cccaaatccc atatttgaat
     1801 aatgttacaa atgccagtgc ttcgataata ctattttgtt tttgaattaa tatcaatgag
     1861 atagaaataa ctaagtataa tattgcagtt gggctatctg gaacaaaaat cttaaaatgc
     1921 cacggcgtat gacttaattg ttcaccatac catatataac cataaatcat ccctaatata
     1981 ttacaaatga gtagcatcat taaccaagaa cgttgataaa gtgtatattg ccaaaatgta
     2041 ttaatcgtca tctgctaagt cctcaaattg attatgttta tttactagct tgagtgtatt
     2101 taaaatttgc gttagttgat aaaaacgttg cttttcattc atctgtaaac ttaaatcaat
     2161 attgtgtaac aagtaatcta ttaataacgc atgtttatgc cgatctatag ccatactatt
     2221 taagtcatga agataagttt gataactggg cgattccgtt agttgagcaa ctattaaatc
     2281 atctagcctt tgctcacgct ttgacacgtt tgcgaagtga atttgaatat caaaagcaca
     2341 gttatgatta gcgatataat caaatatttc atttgtattc attaacttta tattacgctt
     2401 agtaaattga attgcagaag cgtgacttcc cacttctgca atttctaatg tttcatgatg
     2461 attaattttt gtatctacaa aatgaatgtt tgccaatttc gcctcattca cttttatata
     2521 gttaagcacc caaactgcaa tacgcgactt aaatcgatat tgaaaaagta aatattcaat
     2581 aaaactttct ttaatttgat tgagtgtctc tgacatcaaa taccccattt taagattgca
     2641 atcttgataa ttcgtcatgc caattttcgt tacttggctc tagttccaac aattgattta
     2701 aaatagtaat tgcttgttcc ttttgaccaa tttcaattaa atagaaataa taatcactca
     2761 taaaatcaat atttgttttc atcgttggat atgctaattc aaagaaatgt tgagcttctt
     2821 tatctcgctc ttcttgacca taggcgaacg ctaaatgcca catgaatgta ggatccaaat
     2881 cttcttcatc tacatatgtt aataattcaa tgattgcttc ataatcttct tcattacgat
     2941 ataaatcgct taaaatcaat aaagattctt ggtaagcatt atcaacctct aatgcttgtt
     3001 ttaacaataa tacaccttca ttagcatcgc cgtgttctat ttccaaacat ccagttgtat
     3061 acattaactc tttataaaat tgacttagtc gtaatccttc tttacccgtc tcaatggcat
     3121 ctggataatt tttttcattt tcatataatg attgtaaata caagtagcct tgaatataat
     3181 caggatcttt agaaagtaat gtagtcatta ttttaattgc ttcttgagtg atgtcatttt
     3241 tatcgtaaga aatggctttt ttgagataat cttctgaagt catttcatct tcattaattt
     3301 catcgtataa gcgaatcgca tcactatagt taccactttg taaactacaa tctgccatac
     3361 gagagaataa gtttacacca ttaacttgat attcaccagt ttctaaaacg gtttcgtatt
     3421 cagaggtagc acgtaaatat tgaccatcat aatataacat ttcagccaat gcaaaatgga
     3481 ttattggatc atttggctct agttcaagtg cttcttgtaa tttatcaata gcaacttcca
     3541 tcatattaat ttgttgatat aaatctgctt ctaacatcaa cttttcaggt gatggttcaa
     3601 cataacttaa atattctaac gcttcgtcag tttgattttc agacattaaa ccttcaataa
     3661 aataaatcag caattcactt tcgtctggat atttgtgata taacacgcgg aatacttcca
     3721 aaccttgtgg cattaatcca aaattgtaaa gtgtctctcc tagaataaat aatgcgtcat
     3781 cgttgtcagt agttattgct tcattaacac gagagtctaa attttctagt ttttgtagat
     3841 tgatatcgtc tattaattta tagatatctt ccattttata ttatccctca ttttctaaaa
     3901 gctttagttt tggtaaaaat cctggaaatg atacatttac agcatcaaat tgtttgattt
     3961 tgacaggctc gcttgaaagt agagaagcaa ctgcaagcat cattcctatt cgatgatcag
     4021 ttaaactatc aactgttgca tttgttttaa attctgacgg atgaataatc aatccatcat
     4081 tagttggttg taattcaaac cctaacaagt ttaacatatc agccgttgta tcaattctat
     4141 ttgtttcttt tacttttaat tcctcggcat ctttaattgt actcgtgcca actgcttgtg
     4201 tacaaagtaa tgctattaca ggcagttcat caattgcttt tggaactaat tctccttcga
     4261 ttgttattgg ttgaagcatt ggtgtgtatt gaatacgaat agaagcagta ggttcagcac
     4321 cagttgtttg attgaaaagt tggatattac cgcccatttt ttcaacaata tcaataatac
     4381 ctgaacgtgt tggattgatt ccaacattat gaattgttac atcactttct ggtgtgataa
     4441 gtgctgcaac aataaagaac gctgcagatg aaatatcgcc aggaacatga aaatctgcag
     4501 gtttaatatg ttgaatggca tcaggggttg tagttattga taacctttct gcttcaattg
     4561 gaatattaaa atgtctgaac atcgtctcag tatgatttcg acttacatct aattccttta
     4621 ttaccgttgt atcatttgaa aacaaacttg caaataaaat ggcacttttt acttgtgcac
     4681 ttgcaacttc catttgataa tttatacctt ttatgacaga tggcttaata attaatggtg
     4741 tataattatc ttcaatacct tcaatattcg catccataag tttcaatggt ctcaagacac
     4801 gatccattgg ccttttacca attgaaacat cgccagacaa aacactttca atacctaaac
     4861 cacttaacaa accagctaac aatcgagtcg ttgtgccaga gtttccagta tataaaactt
     4921 gatgaggtgt tttaaaagct ttatatccag gtgaattcac aaccaattta tcttcatctt
     4981 ctttaatatc tacgcctaat aatcggaata tatccattgt acgacgacaa tcttcaccaa
     5041 gtaatggttt ataaatattg gaagttcctt cagctaaaga tgctaacatg attgctcgat
     5101 gtgtcatcga cttatctcca ggcacttcta tttcaccttt aagtggccct gaaatatcga
     5161 taatttgttc acttaccatt tacttcacct acttaaaata tgtttttagt tgttcacacg
     5221 catgttgcag tgttgtttga tcaatgtgtt gcactacaat atcgccaaaa tgtttaatca
     5281 ataccatttg aactccttgt ttgtcgttct ttttatcact tagcatgtat tgatatatcg
     5341 tttcgaaatc tatgtcggtt attgtttcta aaggataacc taatttcgtt aaataattaa
     5401 tataatgttg gatatcgtga ttagaattga acaatatatt cgcaacaata aattgatata
     5461 ttatgcctat cattacggca tgaccgtgtg caattttgtg gttatactcc acggcatgac
     5521 caaatgtatg acctaaattt aaaaatttac gtacaccttg ttctttttca tctgcaacaa
     5581 caatatccag cttcgtttca atacctttag caatatattt atccatacca tttaatgact
     5641 gtaatatctc tctatcttta aagtgctgtt cgatttcttg cgtcgttgat tcaccattca
     5701 ataacgcatg cttataaact tctgcatagc cacttaatat ttgctcaaat ggtaacgtct
     5761 ttaaaaagtc taaatcataa atcacagcag ttggacgata aaatgcaccg ataaggtttt
     5821 taccttgttt tgagttaata cccactttac cgccaacact agaatcatgc gccaaaatcg
     5881 ttgtaggaac ttgtataaaa tggacacctc ttaatagtgt tgctgctaca aatcctgcaa
     5941 aatctcctgt cgcaccacca ccaacagcta taatcgctgt attacgcgtt acatgatgtg
     6001 acaaaatgta ttctaatgtt tcttgatatt gctcaaatgt tttcgtcttt tcaccagctg
     6061 gaataatgac tttatgcaca ttttcatatg ataaaatatc atcaaattta tcagcaaaat
     6121 attgatttac atgctcgtca attaatataa aactttgatc aaactgatca atatacgtgc
     6181 taatatggtc aattgcacca tgttcaacaa atattggata attgtttgaa ggataggttg
     6241 tttgtaattt catgatcaca cctcaattgt tctcgttgtt aaaactcaac atttaattgt
     6301 ctgcggtcag caatttgttg ttgaagttgt tcaatatggt ttgattgaaa ctcttcaagt
     6361 aatgctttcg ctatttcaaa tgcaacaacg tgttcacaca ctatacttgc tgcaggtaca
     6421 gcacaactat cagaacgttc aattgttgcc ttaaagtctt ctttagtatt tatatcaact
     6481 gaatttaatg gtttatataa cgtaggaata ggtttcatta caccatttac aataattggc
     6541 ataccatttg acataccacc ctctaaacca cctaaatgat tagatccacg ataataacca
     6601 atttcactat tatatagaat ttcatcttga atctcactac ctggcttttc agctgcttta
     6661 aatccttctc caaagcttac acctttaaaa gcatttatgc tgacaacacc ttgtgcaatc
     6721 ttaccatcta acttacgatc ataatgcaca taactaccaa caccaacagg catattttca
     6781 actacaactt gaacgacacc gccaattgaa tctccttcat ttttagcttc gtcaatttta
     6841 tctcgcattg cttgtgcgat actgtcatca attacacgaa catcattacg atcaagattt
     6901 gctttaaatg tttctgaatc ataaaaatct ttatctttaa tgccacctat ttcaacaaca
     6961 cgactgtata tatcgatatc taactgttgt aataacactt tacataaggc accgactgca
     7021 actcgagctg ctgtttctct agcagatgat cgctctagca catttcgtaa atcacgatga
     7081 ttatatttca tacctccaac caaatctgca tgaccaggtc ttggttttgt aatagtacgt
     7141 ttcatatttt cacgttcttc ttcacttatt ggagctgctc ccataatttt tctccaatgc
     7201 gtaaagtcat cattggttac aaccatagta attggactac ctaatgtata accatttcta
     7261 acgcctgata ctatttctac tgtatctttc tcaatttgca tgcgtcggcc acgaccataa
     7321 cccccttgac gcttgaacat ttctttatta atatcttcaa ctttaatttc taaatttgct
     7381 ggtatacctt cgacaataac tgttaattga ggtccatgtg attctcctga agttagatat
     7441 ctcatacaca ctcgctcctt tcatacttcc gcaataacat tataatatgt tgttaatctt
     7501 atcacattca ttgtggattc ataaatgttt gttttcaagc ttaattaaga aaatggatga
     7561 catctcattt taaatgtcag aattatctta atattaaacg aactaaaatt aattataata
     7621 tattcgtctg tagaaaag
//
LOCUS       DAHHSC010000114         7496 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.115, whole genome shotgun sequence.
ACCESSION   DAHHSC010000114 DAHHSC010000000
VERSION     DAHHSC010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 7496)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7496)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7496
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.115"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            39..935
                     /gene="xerC"
                     /locus_tag="NBI57_001803"
     CDS             39..935
                     /gene="xerC"
                     /locus_tag="NBI57_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HCD1822738.1"
                     /translation="MNHIQEAFLNTLKVERNFSEHTLKSYQDDLIQFNQFLEQEHLQL
                     KTFEYRDARNYLSYLYSNHLKRTSVSRKISTLRTFYEYWMTLDENIINPFVQLVHPKK
                     EKYLPQFFYEEEMEALFKTVEEDTSKNLRDRVILELLYATGIRVSELVNIKKQDIDFY
                     ANGVTVLGKGSKERFVPFGAYCRQSIENYLEHFKPIQSCNHDFLILNMKGEAITERGV
                     RYVLNDIVKRTAGVSEIHPHKLRHTFATHLLNQGADLRTVQSLLGHVNLSTTGKYTHV
                     SNQQLRKVYLNAHPRAKKENET"
     gene            932..1477
                     /gene="hslV"
                     /locus_tag="NBI57_001804"
     CDS             932..1477
                     /gene="hslV"
                     /locus_tag="NBI57_001804"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HCD1822739.1"
                     /translation="MSNTTLHATTIYAVRHNGKAAMAGDGQVTLGQQVIMKQTARKVR
                     RLYEGKVLAGFAGSVADAFTLFEKFETKLQQFSGNLERAAVELAQEWRGDKQLRQLEA
                     MLIVMDKDAILVVSGTGEVIAPDDDLIAIGSGGNYALSAGRALKRHASHLSAEEMAYE
                     SLKVAADICVFTNDNIVVETL"
     gene            1543..2946
                     /gene="hslU"
                     /locus_tag="NBI57_001805"
     CDS             1543..2946
                     /gene="hslU"
                     /locus_tag="NBI57_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HCD1822740.1"
                     /translation="MDTAGIRLTPKEIVSKLNEYIVGQNDAKRKVAIALRNRYRRSLL
                     DEESKQEISPKNILMIGPTGVGKTEIARRMAKVVGAPFIKVEATKFTEVGYVGRDVES
                     MVRDLVDVSVRLVKAQKKSLVQDEATAKANEKLVKLLVPSMKKKASQTNNPLESLFGG
                     AIPNFGQNNEDEEEPPTEEIKTKRSEIKRQLEEGKLEKEKVRIKVEQDPGALGMLGTN
                     QNQQMQEMMNQLMPKKKVEREVAVETARKILADSYADELIDQESANQEALELAEQMGI
                     IFIDEIDKVATNNHNSGQDVSRQGVQRDILPILEGSVIQTKYGTVNTEHMLFIGAGAF
                     HVSKPSDLIPELQGRFPIRVELDSLSVEDFVRILTEPKLSLIKQYEALLQTEEVTVNF
                     TDEAITRLAEIAYQVNQDTDNIGARRLHTILEKMLEDLSFEAPSMPNAVVDITPQYVD
                     DKLKSISTNKDLSAFIL"
     gene            2971..3744
                     /gene="codY"
                     /locus_tag="NBI57_001806"
     CDS             2971..3744
                     /gene="codY"
                     /locus_tag="NBI57_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HCD1822741.1"
                     /translation="MSLLSKTRELNTLLQKHKGIAVDFKDVAQTISSVTVTNVFIVSR
                     RGKILGSSLNELLKSQRIIQMLEERHIPSEYTERLMEVKQTESNIDIDNVLTVFPPEN
                     RELFIDSRTTIFPILGGGERLGTLVLGRVHDDFNENDLVLGEYAATVIGMEILREKHS
                     EVEKEARDKAAITMAINSLSYSEKEAIEHIFEELGGTEGLLIASKVADRVGITRSVIV
                     NALRKLESAGVIESRSLGMKGTFIKVKKEKFLDELEKSK"
     gene            3818..3892
                     /locus_tag="NBI57_001807"
     CDS             3818..3892
                     /locus_tag="NBI57_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1822742.1"
                     /translation="MTLMIMLKSFLYFCLIFNQIDISH"
     gene            4091..4858
                     /gene="rpsB"
                     /locus_tag="NBI57_001808"
     CDS             4091..4858
                     /gene="rpsB"
                     /locus_tag="NBI57_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015900141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HCD1822743.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMKKYIFTERNGIYIIDLQ
                     KTVKKVDEAYNFLKQVSEDGGQVLFVGTKKQAQESVKSEAERAGQFYINQRWLGGLLT
                     NYKTISKRIKRISEIEKMEEDGLFEVLPKKEVVELKKEYDRLIKFLGGIRDMKSMPQA
                     LFVVDPRKERNAIAEARKLNIPIVGIVDTNCDPDEIDYVIPANDDAIRAVKLLTAKMA
                     DAILEGQQGVSNEEVAAEQNIDLDEKEKSEETEATEE"
     gene            4892..5005
                     /locus_tag="NBI57_001809"
     CDS             4892..5005
                     /locus_tag="NBI57_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1822744.1"
                     /translation="MGDKILMLITFFKKKIEANYKYSIRVLAILPIIMLKS"
     gene            5040..5921
                     /locus_tag="NBI57_001810"
     CDS             5040..5921
                     /locus_tag="NBI57_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HCD1822745.1"
                     /translation="MATISAKLVKELRKKTGAGMMDCKKALTETDGDIDKAIDYLREK
                     GIAKAAKKADRIAAEGLVHVETKGNDAVIVEINSETDFVARNEGFQELVKEIANQVLD
                     TKAETVEALMETTLPNGKSVDERIKEAISTIGEKLSVRRFAIRTKTDNDAFGAYLHMG
                     GRIGVLTVVEGSTDEEAARDVAMHIAAINPKYVSSEQVSEEEINHEREVLKQQALNEG
                     KPENIVEKMVEGRLRKYLQEICAVDQDFVKNPDVTVEAFLKTKGGKLVDFVRYEVGEG
                     MEKREENFADEVKGQMK"
     gene            6058..6780
                     /locus_tag="NBI57_001811"
     CDS             6058..6780
                     /locus_tag="NBI57_001811"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000057332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HCD1822746.1"
                     /translation="MAQISKYKRVVLKLSGEALAGEKGFGINPVIIKSVAEQVAEVAK
                     MDCEIAVIVGGGNIWRGKTGSDLGMDRGTADYMGMLATVMNALALQDSLEQLDCDTRV
                     LTSIEMKQVAEPYIRRRAIRHLEKKRVVIFAAGIGNPYFSTDTTAALRAAEVEADVIL
                     MGKNNVDGVYSADPKVNKDAVKYEHLTHIQMLQEGLQVMDSTASSFCMDNNIPLTVFS
                     IMEEGNIKRAVMGEKIGTLITK"
     gene            6799..7353
                     /gene="frr"
                     /locus_tag="NBI57_001812"
     CDS             6799..7353
                     /gene="frr"
                     /locus_tag="NBI57_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HCD1822747.1"
                     /translation="MSDIINETKSRMQKSIESLSRELANISAGRANSNLLNGVTVDYY
                     GAPTPVQQLASINVPEARLLVISPYDKTSVADIEKAIIAANLGVNPTSDGEVIRIAVP
                     ALTEERRKERVKDVKKIGEEAKVSVRNIRRDMNDQLKKDEKNGDITEDELRSGTEDVQ
                     KATDNSIKEIDQMIADKEKDIMSV"
BASE COUNT         2837 a         1055 c         1494 g         2110 t
ORIGIN      
        1 gtatatgcta caattcttat tgatggaggg taaatgtatt gaatcatatt caagaagcgt
       61 ttttaaatac attgaaagtt gaacggaatt tttcggaaca tacattgaaa tcatatcaag
      121 atgacttaat tcagtttaat caatttttag aacaagaaca tttacagttg aaaacttttg
      181 aatacagaga tgctagaaat tatttgagct atttatattc aaatcatttg aaaagaacat
      241 ctgtttctcg taaaatctca acgttaagaa ctttctatga atattggatg acgcttgatg
      301 agaacattat taatccattt gttcaattag tacatccgaa aaaagaaaaa tatcttccgc
      361 aattctttta cgaagaagaa atggaagcgt tattcaaaac tgtagaagag gacacttcaa
      421 aaaatttacg ggatcgagtt attcttgaat tgttgtatgc tacaggcatc cgtgtttcgg
      481 aattagtaaa tattaaaaaa caagatatag atttttacgc gaatggtgtt accgtattag
      541 gaaaagggag caaagagcgt tttgtaccgt ttggtgctta ttgtagacaa agcatcgaaa
      601 attatttaga acatttcaaa ccaattcagt catgcaatca tgattttctt attttaaata
      661 tgaagggtga agcaatcact gaacgcggtg tacgatatgt tttaaatgat attgttaaac
      721 gaacagcagg cgtaagtgag attcatcccc acaagctcag acatacattt gcaacgcatt
      781 tattgaatca aggtgcagac ctaagaacag tacaatcgtt attaggtcat gttaatttgt
      841 caacaactgg taaatataca cacgtatcta accaacaatt aagaaaagtg tatctaaatg
      901 cacatcctcg agcgaaaaag gagaatgaaa catgagtaat acaacattac atgcaacaac
      961 aatttatgct gtaagacata atgggaaagc agctatggct ggagatggac aagtaacgct
     1021 tggtcaacaa gtcatcatga aacaaacggc aagaaaagtg cgacgtttat atgaaggtaa
     1081 agtgttagct ggtttcgcag gtagtgtagc agatgcgttt acgttatttg aaaaatttga
     1141 aacaaaatta caacagttta gtggtaactt agaaagagct gctgttgaat tggcacaaga
     1201 atggcgaggc gataaacaat tacgtcaatt agaagctatg ctaattgtaa tggataaaga
     1261 tgctatttta gttgtcagtg gaactggcga agttattgcc ccagatgatg accttatcgc
     1321 tattggatca gggggcaact acgcattaag cgcaggacgt gcattgaaac gccatgcatc
     1381 gcatttgtct gctgaagaaa tggcatatga gagcttgaaa gtagcggctg atatttgtgt
     1441 ctttaccaac gataatattg ttgttgaaac actataataa tcagagcacg ataaataatt
     1501 acgaccaatt aattttagtt aaaagacgga ggaatgaaat taatggatac agctggaata
     1561 agattaactc caaaagaaat cgtatctaaa ttaaatgaat atatcgttgg acaaaatgat
     1621 gctaaacgta aagtggcaat tgccctacgt aatcgataca gaagaagttt attagatgag
     1681 gaatcaaagc aagaaatttc acctaaaaat attttgatga ttggaccaac cggcgttggt
     1741 aaaactgaaa ttgcaagaag aatggccaaa gttgtcggcg cgccatttat aaaagtagaa
     1801 gctactaaat ttactgaggt aggttatgta ggacgagatg ttgaaagtat ggttagagat
     1861 cttgttgatg tttcagtaag attagtcaag gcgcagaaaa aatcattggt acaagatgaa
     1921 gcaacagcta aggccaatga aaaacttgtt aagttattag ttccaagtat gaaaaagaaa
     1981 gcgtctcaaa cgaataatcc tttagagtca cttttcggag gtgcaattcc aaatttcgga
     2041 caaaataacg aagatgaaga agaaccacct actgaggaaa ttaaaacaaa acgttctgaa
     2101 attaagagac agctagaaga aggcaaactt gaaaaagaaa aggtaagaat taaagtcgaa
     2161 caagatcctg gtgctttagg tatgctaggt acaaatcaaa atcagcaaat gcaagagatg
     2221 atgaatcaat taatgcctaa aaagaaagtt gagcgagaag ttgctgttga gacggcaagg
     2281 aaaatcttag ctgatagtta tgcggatgaa ctaattgatc aagaaagcgc taaccaagaa
     2341 gcgcttgaat tagcagaaca aatgggtatc atctttatag atgaaatcga caaagttgcg
     2401 acgaataatc ataatagtgg tcaagatgtc tcaagacaag gtgttcaaag agatatttta
     2461 cctatacttg aaggtagcgt tattcaaacc aaatatggta ctgtgaatac tgaacatatg
     2521 ctgtttatag gtgctggagc tttccatgta tctaagccga gtgacttgat accagaattg
     2581 caaggtcgtt ttccgattag agttgaactt gatagtttat cggtagaaga ttttgtaaga
     2641 attttgacag aaccaaaatt gtcattaatt aaacaatatg aagcattgct tcaaacagaa
     2701 gaagttactg taaactttac cgatgaagca attactcgct tagctgagat tgcttatcaa
     2761 gtaaatcaag atacagacaa cattggtgca cgtcgacttc atacaatttt agaaaagatg
     2821 ctagaagatt tatcattcga agcaccaagt atgccgaatg cagttgtaga tattacccca
     2881 caatatgttg atgataaatt aaaatcaatt tcaacaaata aagatttaag tgcatttatt
     2941 ctataaaaat atacaaaagg agaaaaattc atgagcttat tatctaaaac gagagagtta
     3001 aacacgttac ttcaaaaaca caaaggtatt gcggttgatt ttaaagatgt agcacaaacg
     3061 attagtagcg taactgtaac aaatgtattt attgtatcgc gtcgaggtaa aattttaggg
     3121 tcgagtctaa atgaattatt aaaaagtcaa agaattattc aaatgttgga agaaagacat
     3181 attccaagtg aatatacaga acgattaatg gaagttaaac aaacagaatc aaatattgat
     3241 atcgacaatg tattaacagt attcccacct gaaaacagag aattattcat agatagtcgt
     3301 acaactatct tcccaatttt aggtggagga gaaagattag gtacattagt acttggtcga
     3361 gtacatgatg attttaatga aaatgatttg gtactaggtg aatatgctgc tacagttatt
     3421 ggtatggaaa tcttacgtga gaagcatagt gaagtagaaa aagaagcgcg cgataaagct
     3481 gctattacaa tggcaattaa ttcattatct tattctgaaa aagaagcgat tgaacatatc
     3541 tttgaagaac ttggcggtac ggaaggccta ttaatcgcat caaaagttgc agatagagtt
     3601 ggtattacta gatctgtaat tgtaaatgca ctacgtaaat tagaaagtgc tggtgtaatt
     3661 gaatcacgtt ctttaggaat gaaaggtact ttcattaaag ttaaaaaaga aaaattctta
     3721 gatgaattag aaaaaagtaa ataagtcgat gagtctggga cataattcaa tgtcctagtc
     3781 gtgacgatgt tatcttggca gtagtcaact caacgaaatg actttgatga ttatgttgaa
     3841 gtcatttctt tatttttgtc taattttcaa tcaaattgat atttcacatt aacttgatag
     3901 aattaccttt aaatttatgg aaaaatgctt gaaaaacatt tgctttagac acggcattat
     3961 gttatattta ttttcggcta taaagccaat acacacatat caaacgtgat tgtgtaaagg
     4021 gtgcaatatt tatatattgt ctttacaata gtttgctatg gaggtaatta accaatagga
     4081 ggaatttata atggcagtaa tttcaatgaa acaattacta gaagcgggtg ttcacttcgg
     4141 tcaccaaaca cgtcgttgga acccaaaaat gaaaaaatat atcttcactg agagaaatgg
     4201 tatttatatc atcgacttac aaaaaacagt gaaaaaagta gacgaggcat acaacttctt
     4261 gaaacaagtt tcagaagatg gtggacaagt cttattcgta ggaactaaaa aacaagcaca
     4321 agaatcagtt aaatctgaag cagaacgtgc tggtcaattc tacattaacc aaagatggtt
     4381 aggtggatta ttaactaact ataaaacaat ctcaaaacga atcaaacgta tttctgaaat
     4441 tgaaaaaatg gaagaagatg gtttattcga agtattacct aaaaaagaag tagtagaact
     4501 taaaaaagaa tacgaccgtt taatcaaatt cttaggcgga attcgtgata tgaaatcaat
     4561 gcctcaagca ttattcgtag ttgacccacg taaagagcgt aatgcaattg ctgaagctcg
     4621 taaattaaat attcctatcg taggtatcgt tgacactaac tgtgatcctg acgaaattga
     4681 ctacgttatc ccagcaaacg acgatgctat ccgtgcggtt aaattattaa ctgctaaaat
     4741 ggcagatgca atcttagaag gtcaacaagg cgtttctaat gaagaagtag ctgcagaaca
     4801 aaacatcgat ttagatgaaa aagaaaaatc agaagaaaca gaagcaactg aagaataatc
     4861 aactgttgaa tctgacttag atatagttta aatgggtgat aagatattaa tgcttatcac
     4921 cttttttaaa aagaaaatcg aggcaaatta caaatattca attagagtat tggcaatctt
     4981 gcctataata atgctaaaat cataatatat aaaatgataa cttattggag gaataatgaa
     5041 tggcaactat ttcagcaaaa cttgttaaag aattacgtaa aaaaactggc gcgggtatga
     5101 tggattgtaa aaaagcgcta actgaaactg atggtgacat cgataaagcg attgattacc
     5161 tacgtgaaaa aggtattgct aaagcagcta aaaaagcaga ccgtattgcg gctgaaggtt
     5221 tagtacatgt agaaactaaa ggtaacgacg cagttatcgt tgaaatcaac tctgaaacag
     5281 actttgttgc tcgtaacgaa ggattccaag agttagttaa agaaatcgct aatcaagtat
     5341 tagatacaaa agctgaaact gttgaagctt taatggaaac aactttacca aatggtaaat
     5401 cagttgatga aagaattaaa gaagcaattt caacaatcgg tgaaaaatta agtgttcgtc
     5461 gttttgctat cagaactaaa actgataacg atgctttcgg cgcttactta cacatgggtg
     5521 gacgcattgg tgtattaaca gttgttgaag gttcaactga cgaagaagca gcaagagacg
     5581 ttgctatgca tatcgctgca atcaacccta aatatgtttc ttctgaacaa gttagcgaag
     5641 aagaaatcaa ccacgaaaga gaagttttaa aacaacaagc attaaatgaa ggtaaaccag
     5701 aaaacatcgt tgaaaaaatg gtggaaggac gtttacgtaa atacttacaa gaaatttgtg
     5761 ctgtagatca agacttcgtt aaaaaccctg atgtaacagt tgaagctttc ttaaaaacaa
     5821 aaggtggaaa acttgttgac ttcgtacgct atgaagtagg cgaaggtatg gaaaaacgcg
     5881 aagaaaactt tgcggatgaa gttaaaggac aaatgaaata atctgtcata aagtaaaaca
     5941 aggaagaaga cacctttaat gttgctttat taaaatgtaa atcattctaa taaaacgaca
     6001 actgtgtctt ctttacttgt atatgttaca tatattcacg atagagagga taagaaaatg
     6061 gctcaaattt ctaaatataa acgtgtagtt ttgaaactaa gtggtgaagc gttagctgga
     6121 gaaaaaggat ttggcataaa tccagtaatt attaaaagtg ttgctgagca agtggctgaa
     6181 gttgctaaaa tggactgtga aatcgcagta atcgttggtg gcggaaacat ttggagaggt
     6241 aaaacaggta gtgacttagg tatggaccgt ggaactgctg attacatggg tatgcttgca
     6301 actgtaatga atgccttagc attacaagat agtttagaac aattggattg tgatacacga
     6361 gtattaacat ctattgaaat gaagcaagtg gctgaacctt atattcgtcg tcgtgcaatt
     6421 agacacttag aaaagaaacg cgtagttatt tttgctgcag gtattggaaa cccatacttc
     6481 tctacagata ctacagcggc attacgtgct gcagaagttg aagcagatgt tattttaatg
     6541 ggcaaaaata atgtagatgg tgtatattct gcagatccta aagtaaacaa agatgcggta
     6601 aaatatgaac atttaacgca tattcaaatg cttcaagaag gtttacaagt aatggattca
     6661 acagcatcct cattctgtat ggataataac attccgttaa ctgttttctc tattatggaa
     6721 gaagggaata ttaaacgtgc tgttatgggt gaaaagatag gtacgttaat tacaaaataa
     6781 atttagaggt gtaaaataat gagtgacatt attaatgaaa ctaaatcaag aatgcaaaaa
     6841 tcaatcgaaa gcttatcacg tgaattagct aatatcagtg caggaagagc taattcaaat
     6901 ttattaaacg gcgtaacagt tgattactat ggtgcaccaa cacctgtaca acaattagca
     6961 agcatcaatg ttccagaagc acgtttactt gttatttctc catacgacaa aacttctgta
     7021 gctgacatcg aaaaagcgat aatagcggct aacttaggtg tcaacccaac aagtgatggt
     7081 gaagtgatac gtattgcagt acctgcctta acagaagagc gtagaaaaga gcgcgttaaa
     7141 gatgttaaga aaattggtga agaagctaaa gtatctgttc gaaatattcg tcgtgatatg
     7201 aatgatcagt tgaaaaaaga tgaaaaaaat ggcgacatta ctgaagatga gttgagaagt
     7261 ggtactgaag atgttcagaa agcaacagac aattcaataa aagaaattga tcaaatgatt
     7321 gctgataaag aaaaagatat tatgtcagta taaaactaat atacaatgac atattaaaat
     7381 gccagtatta aacgataatg taacatttaa aatgggcatg tttaattaaa tcaaagatgc
     7441 atgtgataat ttaaattcac aatgagcata aaaatggtgt ttaaacaagt taatta
//
LOCUS       DAHHSC010000115         7485 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.116, whole genome shotgun sequence.
ACCESSION   DAHHSC010000115 DAHHSC010000000
VERSION     DAHHSC010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 7485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7485
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.116"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            77..1270
                     /gene="sfaA"
                     /locus_tag="NBI57_001813"
     CDS             77..1270
                     /gene="sfaA"
                     /locus_tag="NBI57_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphyloferrin A export MFS transporter"
                     /protein_id="HCD1822748.1"
                     /translation="MTKYFFSSSFLLFLGNWIGQIGLNWFVLTTYHNAVYLGIVNFCR
                     LVPILLLSVWAGAIADKYDKGRLLRITISSSFLVTAILCVLTYSFTAIPISVIIIYAT
                     LRGILSAVETPLRQAILPDLSDKISTTQAVSFHSFIINICRSIGPAIAGVILAVYHAP
                     TTFLAQAICYFIAALLCLPLHFKVTKIPEDATRYMPLKVIIDYFKLHMEGRQIFITSL
                     LIMATGFSYTTLLPVLTNKVFPGKSEIFGIAMTMCAIGGIIATLVLPKVLKYIGMVNM
                     YYLSSLLFGIALLGVVFHNIVIMFICITLIGLFSQWARTTNRVYFQNNVKDYERGKVL
                     SIIMMDRGMIPLGSLLMSICADVFGIVRTFSIMGISTICITMVFYFINRKLKLKLEES
                     NHGIS"
     gene            1257..3014
                     /gene="sfaB"
                     /locus_tag="NBI57_001814"
     CDS             1257..3014
                     /gene="sfaB"
                     /locus_tag="NBI57_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphyloferrin A synthetase SfaB"
                     /protein_id="HCD1822749.1"
                     /translation="MVYLEWAKADRNIQYRVINAIIKERIYPEQTFISQKGSLIEIQY
                     HMHVLTIEVVRKSALERYEFTGDITYLNKGETSLIITLEGLLDVLNHDFDIPISERLR
                     EELIHSRDSLVETYKQMSHRQTLISQSFKFSRLPQDINFFSWLQHVKDSDKTDDLTYS
                     ESLVPEGHPTHPLTKTKLPLTMEEVRAYAPEFEKEIPLQIMMIEKDHVVCTAMDGNDQ
                     FIIDEIIPEYYNQIRVFLKSLGLKSEDYRAILVHPWQYDHTIGKYFEAWIAKKILIPT
                     PFTILSKATLSFRTMSLIDKPYHVKLPVDAQATSAVRTVSTVTTVDGPKLSYALQNML
                     NQYPGFKVAMEPFGEYANVDKDRARQLACIIRQKPEIDGKGATVVSASLVNKNPIDQK
                     VIVDSYLEWLNQGITKESITTFIERYAQALIPPLIAFIQNYGIALEAHMQNTVVNLGP
                     HFDIQFLVRDLGGSRIDLETLQHRVSDIKITNDSLIADSIDAVIAKFQHAVIQNQMAE
                     LIHHFNQYDCVEETELFNIVQQVVAHAINPTLPHANELKDILFGPTITVKALLNMRME
                     NKVKQYLNIELDNPIKKEV"
     gene            3021..4091
                     /gene="sfaC"
                     /locus_tag="NBI57_001815"
     CDS             3021..4091
                     /gene="sfaC"
                     /locus_tag="NBI57_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphyloferrin A biosynthesis protein SfaC"
                     /protein_id="HCD1822750.1"
                     /translation="MAHVNINISKIKYNAKVLQTVFQSKNIQFTPVIKCIAGDRTIVE
                     SLKALGINHVAESRLDNIISIADQDLTYTLLRTPAKKEISDMIEKVDMSIQTELSTIH
                     QINEVAEVLGKKHKILLMVDWKDGREGVLTYDVLDYIKEIIHLKNIHFVGLAFNFMCF
                     KSDAPSDDDIFMINRFVSAVEREIGYRLKIISGGNSSMLPQLLYNDLGKINELRIGET
                     LFRGVDTTTNQAIAMLYQDAITLEAEILEIKPRVNTQTHESFLQAIVDIGYLDTKVDN
                     ISPMDQHINILGASSDHLMLDLNGQGHYQVGDHISFSLNYEALSHSMYMKNLHKVYID
                     DSKIDTLLQNFDVKSPAMVNQY"
     gene            complement(4192..4317)
                     /locus_tag="NBI57_001816"
     CDS             complement(4192..4317)
                     /locus_tag="NBI57_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1822751.1"
                     /translation="MYHCYIIFALIQLTPFSMTVIPVIQLILSVAILCDELLHLI"
     gene            4483..5466
                     /locus_tag="NBI57_001817"
     CDS             4483..5466
                     /locus_tag="NBI57_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCD1822752.1"
                     /translation="MRGLKTFSILGLIVALLLVAACGNTDNSSKKESSTKDTISVKDE
                     NGTVKVPKDAKRIVVLEYSFADALAALDVKPVGIADDGKKKRIIKPVREKIGDYTSVG
                     TRKQPNLEEISKLKPDLIIADSSRHKGINKELNKIAPTLSLKSFDGDYKQNINSFKTI
                     AKALNKEKEGEKRLAEHDKLINKYKDEIKFDRNQKVLPAVVAKAGLLAHPNYSYVGQF
                     LNELGFKNALSDDVTKGLSKYLKGPYLQLDTEHLADLNPERMIIMTDHAKKDSAEFKK
                     LQEDATWKKLNAVKNNRVDIVDRDVWARSRGLISSEEMAKELVELSKKEQK"
     gene            5478..6509
                     /locus_tag="NBI57_001818"
     CDS             5478..6509
                     /locus_tag="NBI57_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HCD1822753.1"
                     /translation="MAIKEISSQSAIDHKRKRRTTLTYIVSLCFLFICIYLNMAIGSS
                     KINFSDIIHYVTGHTDTKATFLLHNVRMPRMIAGLFIGGALAVSGLLMQAMTRNPLAS
                     PKIFGVSSGASFFIVFVTIIIPSLEYYALYLGVIGAFIGGLTVYTLSGATKGMTPIKL
                     ALAGMAIHLFFSSMTEGIIILNENSNEQVMFWLVGSLSSMKWDEILTILPWIIGALIV
                     TIFIGRQLTIMELGDDIAKGLGQNINKVRIIIGLLVIILTGMSVSVAGPIGFVGLIVP
                     HIVKRYVSKNYLVMIPLTFIIGADLLLLSDVLSRLITYPYESPVGIVTSFVGALYFLF
                     ITIKGVKRI"
     gene            6506..7474
                     /locus_tag="NBI57_001819"
     CDS             6506..7474
                     /locus_tag="NBI57_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HCD1822754.1"
                     /translation="MMTTKIIRRYSLITILLIVSIFISLCVGSVMIHPIDAIKGIFTQ
                     DDFILNEYRIPRTLLGIIIGSSLAISGAIIQAVIRNPLASPDVIGISKGASLAAVIII
                     MIFPTAPLFVLPIGSFLGAFAVSLFLSFLISKYDVKGSKLALIGLAIGAICTAIVQFL
                     LIRNPLEANKALVWLTGSLYGHDMSNIYTILPWFLITIPIIILLSYQLDVLNLGDAVA
                     TALGLKVKTIKMLLLILAVILAGSAISVVGGISFLGLIAPHIARQLIGNKTLHVIIMS
                     GLIGAILLTFGDGLARGIHPPLDIPVGVIIAIIGAPYFLILLRRMK"
BASE COUNT         2580 a         1019 c         1381 g         2505 t
ORIGIN      
        1 aaaagcaaga tagatttgta taatccatat taatgataat gattcttatt atcaacagaa
       61 tgcgggtgta agttttatga caaaatattt ttttagcagt tcttttctac tatttctagg
      121 taattggatt ggacaaatag ggctaaattg gtttgtactt accacttatc ataacgcagt
      181 ttatctgggg attgtcaatt tttgcagact tgtaccaata ttattactaa gtgtgtgggc
      241 aggggcaatt gccgataaat atgataaagg gcgattgctg agaattacaa tttcatcatc
      301 atttttagta actgcaattt tatgtgtgct cacgtatagt ttcactgcaa ttccaattag
      361 cgtcattatt atatatgcga cattaagagg gattttaagt gcggttgaaa cacctttaag
      421 acaagcaatc ttaccagatt tatcagataa aatatctact acacaagctg tatcatttca
      481 ttcattcatc attaatattt gtcgttcaat agggcctgcc attgctggtg tcatattagc
      541 agtctatcat gcgccaacaa catttcttgc acaggcaatt tgttatttta tcgcagcttt
      601 attatgctta ccattacatt ttaaagtaac taaaatacct gaagatgcaa caagatacat
      661 gccgttaaaa gttattatag attacttcaa attacatatg gaaggtcgac aaatatttat
      721 aacatcatta ttgattatgg cgacaggttt ttcatatacg acacttttac cagttttgac
      781 aaacaaagta tttccgggga aatctgaaat atttggtatc gctatgacga tgtgtgccat
      841 tggtggtatt attgcaacgc tagttttacc taaagtactt aaatatattg gtatggtaaa
      901 tatgtattat ttaagttcac ttttatttgg cattgctttg ttaggtgtgg tatttcacaa
      961 tattgtcatc atgttcattt gtattacatt gattgggtta tttagtcaat gggcacgtac
     1021 gacaaatcgc gtttattttc aaaataatgt taaagattat gaacgtggta aagtactgag
     1081 tattattatg atggatagag gtatgattcc attgggaagt ctgttaatga gtatatgtgc
     1141 agatgtgttt ggcattgtta gaactttttc aataatggga ataagtacta tatgcattac
     1201 aatggtattc tattttataa atagaaagtt gaagttaaag ttggaggaaa gtaatcatgg
     1261 tatatcttga atgggcaaag gcagatagaa atattcaata tcgtgtaatt aacgccatta
     1321 ttaaagaacg tatttacccc gagcaaacat ttatttcgca aaaaggatct ttaattgaaa
     1381 ttcagtatca tatgcatgtg ttgactattg aagttgttag aaaaagtgca ttagaacgct
     1441 atgagtttac aggtgatatt acttatttaa ataaaggtga aacgtcatta attataactt
     1501 tagaaggttt gttagatgtg ttgaatcatg actttgatat ccctatttca gagcgactac
     1561 gcgaagagtt aatacacagt cgagatagtt tagttgaaac atataagcaa atgtctcaca
     1621 gacaaacatt aataagtcaa agttttaaat tttcaagatt accacaagat attaactttt
     1681 tttcatggtt acaacatgta aaagatagtg ataagacaga tgatttaact tattctgaga
     1741 gcttggtacc agaggggcat ccaacacacc ctttaaccaa aacgaaattg cccttaacta
     1801 tggaagaagt acgagcatat gcacctgagt ttgaaaaaga aatccctttg caaattatga
     1861 tgattgaaaa agatcatgtt gtgtgcacag ctatggatgg taatgatcaa tttattattg
     1921 atgaaataat tcccgaatac tacaatcaga ttcgtgtgtt tttaaagagt ttaggtttga
     1981 aaagtgaaga ctatagagcg attttagtac atccttggca atatgatcat acgataggga
     2041 aatattttga agcatggatt gctaaaaaaa tattaattcc aacgccgttt acaatacttt
     2101 caaaagcaac tttatcattt aggacgatgt ctttaattga taaaccatac catgttaagt
     2161 tgcccgtcga tgcacaagca acaagtgccg ttagaacagt ctcaactgtg actactgtag
     2221 atggaccaaa gttaagttat gctttacaaa acatgttgaa tcaatatcca ggatttaaag
     2281 ttgctatgga accgttcggt gaatatgcaa atgttgataa agatagggca cgtcagcttg
     2341 cttgtattat aagacaaaag cctgaaattg atggaaaagg tgcaacagta gttagtgcaa
     2401 gtctagttaa taaaaatcca atagatcaaa aagttatcgt ggatagttac ttagagtggt
     2461 taaatcaagg aattactaaa gaaagtatta cgacatttat tgaacgatac gctcaagcat
     2521 taatcccgcc tttaattgct tttattcaaa attatggaat tgcattagaa gcacacatgc
     2581 aaaatacagt agtgaacttg gggccacatt ttgacattca atttttagtg agagatttag
     2641 gtggttctag aattgattta gaaacattac aacatcgtgt atcagatatt aaaattacaa
     2701 atgatagttt aatagctgat tctatagatg cagtgattgc aaaattccaa catgctgtta
     2761 ttcaaaatca aatggcagaa ttaatccatc attttaatca gtatgattgt gttgaagaaa
     2821 ccgaattatt taacatagta cagcaagtag tagcgcatgc tattaaccca acactaccac
     2881 atgcaaatga gttaaaagat attttgtttg gaccaacaat tactgtcaaa gcgttgttaa
     2941 atatgagaat ggaaaataaa gtaaagcaat atttaaatat tgagttagat aatccgataa
     3001 aaaaagaggt gtagtactac atggcacacg ttaacataaa tatatcgaag attaaatata
     3061 acgccaaagt acttcaaaca gtttttcaaa gtaaaaatat acaattcaca ccagtaatta
     3121 agtgtatagc tggtgaccgt acaattgtag aaagcttaaa agcgttaggt atcaatcatg
     3181 ttgcagaatc cagattggat aacataatta gtattgcaga tcaggattta acatatacgt
     3241 tattgcgtac gcctgccaaa aaagagattt cagatatgat agaaaaagtt gatatgagta
     3301 tccaaacaga attatctacc atacatcaaa taaatgaagt agcagaagtt ttaggaaaaa
     3361 aacataagat tttattaatg gtagattgga aagatggccg tgaaggtgtt ctgacttatg
     3421 atgttttaga ttatattaaa gagattattc acttgaaaaa tatccatttt gtaggtttag
     3481 cattcaattt tatgtgtttt aaatctgatg cgccaagtga tgatgatatc tttatgatta
     3541 atcgattcgt ctcagctgtt gaaagagaaa taggatatcg attgaaaatt atttcaggtg
     3601 gtaattcgag catgttacca caacttttat ataatgattt gggtaaaata aatgaattgc
     3661 gaataggtga aacgttgttt agaggtgttg atacaacgac aaatcaagcg atcgctatgt
     3721 tatatcaaga tgcgattaca ttggaggctg agattttaga aattaaacct cgagttaata
     3781 cacaaacgca tgaatcattc ttacaagcta ttgtagatat tggttattta gatacaaaag
     3841 tcgataatat ttcacctatg gatcagcata tcaatatttt aggcgcttcg agtgatcatt
     3901 taatgcttga tttaaatggt caaggacact atcaagttgg agatcatata tctttttcat
     3961 taaattatga agcactttct cattcgatgt atatgaaaaa tttacataag gtatatattg
     4021 atgattcaaa gattgatacg ctacttcaaa attttgatgt gaaatctcct gctatggtaa
     4081 accaatatta atatatttat caataattct aagttgacaa gttatactac atatcgtttg
     4141 cacggttgta tcataattgt tcaacttaga ttttttgtat ttgttgattt atcaaattaa
     4201 gtgcaacagt tcgtcacata aaattgcaac agataatatc agctgaatta cagggataac
     4261 ggtcatgcta aatggtgtca attgtattaa tgcaaagatg atatagcaat gatacattat
     4321 cagtattttg tctaaggaaa tgtgctaatt gtagtcataa ttattagagg aaatatatag
     4381 tcatacattt tagaaatata aaaaagattg aacgctactt gacaatgata attgttatca
     4441 ataaaataat aaatgaagtt atacatatta aggagtggaa cgatgagagg tctaaaaact
     4501 tttagtatat tgggattaat agttgcctta cttttagttg cagcttgtgg taatacggat
     4561 aattcaagta aaaaagaatc atcaactaaa gatactattt cggtaaaaga tgaaaatggt
     4621 acagtaaaag tacctaaaga tgcaaaacgt atcgttgtat tagagtactc atttgcagat
     4681 gcattagcag cattagacgt taaaccagtt ggtattgctg atgatggtaa gaaaaaacgt
     4741 atcattaaac cagttagaga aaaaattggg gattatactt ctgtaggtac acgtaaacag
     4801 ccaaacttag aggaaattag taaattaaaa ccggatttaa ttatcgctga tagcagtaga
     4861 cataaaggta ttaataaaga attaaacaaa attgcaccaa cattatcatt aaagagtttt
     4921 gatggagact acaaacaaaa tattaattcg ttcaaaacaa ttgctaaagc tttaaataaa
     4981 gaaaaagaag gcgaaaaacg tcttgctgag catgataaat taatcaataa gtataaagat
     5041 gaaattaaat ttgatagaaa tcaaaaagtg cttccagcag tagttgctaa agctggttta
     5101 ttagcacatc caaactattc atatgttgga caatttttaa acgaactagg atttaaaaat
     5161 gcattaagtg acgatgtaac aaaaggttta agtaaatatt tgaaaggacc ttacttacaa
     5221 ttagacactg aacatttagc tgatttaaat ccagagcgta tgatcattat gacagatcat
     5281 gctaaaaaag attctgctga attcaagaag ttacaagaag atgcaacatg gaaaaagttg
     5341 aatgcagtta aaaataatcg cgtggatatt gttgaccgtg atgtttgggc aagatctcgt
     5401 ggcttaattt cttctgaaga aatggctaaa gaacttgttg aattatcaaa aaaagaacaa
     5461 aagtaaggtg gaagtaaatg gctataaaag aaataagtag ccaatctgcc atagatcata
     5521 aaagaaaaag acgcacaaca ctcacgtata tagtgagttt gtgctttctt tttatttgta
     5581 tatatttaaa tatggcgatt ggttcttcga aaattaattt tagcgatatc attcactatg
     5641 ttactggtca tacagatacg aaagcaacgt ttttattgca taatgtacgt atgccaagga
     5701 tgattgcagg gttatttatt ggcggtgcat tagcggtatc tggtttgtta atgcaagcaa
     5761 tgacacgcaa tccgctagca tcacctaaaa tttttggtgt cagttctggt gcatcgtttt
     5821 tcattgtatt tgtgacgatt atcattccat cattagaata ttatgcatta tatttaggtg
     5881 tgataggtgc atttataggt ggtttaactg tttatacact ttcaggtgct acgaaaggta
     5941 tgacacccat taaattagca ttggctggta tggccatcca tttgtttttt agtagtatga
     6001 ctgaaggtat tattattttg aatgagaatt caaacgaaca agtgatgttt tggcttgtcg
     6061 gttcattatc cagtatgaag tgggatgaaa ttttaaccat tttaccatgg attattggtg
     6121 ctttaattgt aacgattttt attggacgtc aactaacgat tatggaacta ggcgatgaca
     6181 ttgctaaggg attgggtcaa aatataaata aagttagaat tatcattggg ctattagtca
     6241 ttattcttac aggtatgtcg gtgtcagtgg caggaccaat tggatttgtt ggcttaatcg
     6301 taccgcatat agtcaaacgc tatgtgagta agaattattt agtcatgatt ccattaacat
     6361 ttattatagg tgcagatttg ttactactat cagatgtatt aagtcgttta atcacttatc
     6421 cttacgaatc acctgtaggt attgtgacat cattcgtagg tgcactttac ttcttattta
     6481 taacgattaa aggggtgaaa agaatatgat gacaacgaaa attatacgtc gttatagttt
     6541 aatcacaatc cttttaattg tgagtatatt cataagttta tgtgtagggt ctgtcatgat
     6601 tcatccgatt gatgctatta agggaatctt tacacaagat gattttattt taaatgagta
     6661 tcgtatacct agaacattac ttggtattat tatcggtagt agcttggcta tatcaggtgc
     6721 aattatacaa gctgtaataa gaaacccgtt agcctcacct gatgttatag gtatttctaa
     6781 aggtgccagt ttagctgctg taattattat tatgatcttt ccaacagcac cattgtttgt
     6841 tttacctata ggttcattct taggtgcctt tgcagtaagt ttatttttat catttttaat
     6901 ttcaaaatat gatgttaaag gttcaaagct tgccttaatt ggtttagcaa taggcgcaat
     6961 atgtacggcg attgtgcagt tcttattaat cagaaatcca ctggaagcaa acaaagcgct
     7021 tgtttggttg acaggtagtc tatatggaca tgatatgtcg aatatttata ctatattacc
     7081 ttggttttta ataacgatac caatcatcat tttattgagt tatcaattag atgtattgaa
     7141 tctaggtgat gctgttgcaa ccgcactcgg tttaaaagtt aaaactatta aaatgctatt
     7201 gctaatttta gcagtaatat tagcaggatc cgcaatttca gttgtcggtg gtattagctt
     7261 tttaggatta attgcacctc atattgcgag acaattaata ggaaataaaa cattacacgt
     7321 tattatcatg tcggggttaa ttggtgctat attgttaaca tttggagatg gtttagcccg
     7381 tggcattcat ccgccactgg atattcctgt aggggttatc attgcaatta tcggtgcacc
     7441 atacttctta atattattac gaagaatgaa ataacggttc ttcat
//
LOCUS       DAHHSC010000116         7284 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.117, whole genome shotgun sequence.
ACCESSION   DAHHSC010000116 DAHHSC010000000
VERSION     DAHHSC010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 7284)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7284)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7284
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.117"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            147..1187
                     /locus_tag="NBI57_001820"
     CDS             147..1187
                     /locus_tag="NBI57_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HCD1822755.1"
                     /translation="MIIAIIILIFISFFFSGSETALTAANKTKFKTEADKGDKKAKGI
                     VKLLEKPSEFITTILIGNNVANILLPTLVTIMALRWGISVGIASAVLTVVIILISEVI
                     PKSVAATFPDKITRLVYPIINICVIVFRPITLLLNKLTDSINRSLSKGQPQEHQFSKE
                     EFKTMLAIAGHEGALNEIETSRLEGVINFENLKVKDVDTTPRINVTAFASNATYEEVY
                     ETVMNKPYTRYPVYEGDIDNIIGVFHSKYLLAWSNKKEDQITNYSAKPLFVNEHNKAE
                     WVLRKMTISRKHLAIVLDEFGGTEAIVSHEDLIEELLGMEIEDEMDKKEKEKLSQQQI
                     QFQQRKNRNVSI"
     gene            1201..2268
                     /locus_tag="NBI57_001821"
     CDS             1201..2268
                     /locus_tag="NBI57_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HCD1822756.1"
                     /translation="MWNKNRLTQMLSIEYPIIQAGMAGSTTPKLVASVSNSGGLGTIG
                     AGYFNTQQLEDEIDYVRQLTSNSFGVNVFVPSQQSYTSSQIENMNAWLKPYRRALHLE
                     EPVVKIIEEQQFKCHIDTIIKKQVPVCCFTFGIPNESIIERLKEANIKLIGTATSVDE
                     AIANEKAGMDAIVAQGSEAGGHRGSFLKPKNQLPMVGTISLVPQIVDVVSIPVIAAGG
                     IMDGRGVLASIVLGAEGVQMGTAFLTSQDSNASELLRDAIINSKETDTVVTKAFSGKL
                     ARGINNRFIEEMSQYEGDIPDYPIQNELTSSIRKAAANIGDKELTHMWSGQSPRLATT
                     HPANTIMSNIINQINQIMQYK"
     gene            2522..2605
                     /locus_tag="NBI57_001822"
     CDS             2522..2605
                     /locus_tag="NBI57_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1822757.1"
                     /translation="MIIIKRKHQAEDRRKILIKRKHQIKVR"
     gene            2653..3501
                     /locus_tag="NBI57_001823"
     CDS             2653..3501
                     /locus_tag="NBI57_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HCD1822758.1"
                     /translation="MINIGLTGWGDHYSLYEDLERQTDKLKTYAGHFPVVELDATYYA
                     IQPERNILKWIKETPDTFEFVVKIHQALTLHADYKTFADTRQELFDQFKNMLEPLHTQ
                     KKLAMVLVQFPPWFDCNAQNIKYILYVRQQLQAFPMCVEFRHQSWFSDAFKEQTLAFL
                     TEHQIIHAVVDEPQVKDGSVPLVNRITNEIAFVRYHGRNHYGWTKKDMSDQEWRDVRY
                     LYDYNEQELIDLAQKAQILAQKAKKVYVIFNNNSGGHAANNAKTYQRLLNIEYEGLAP
                     QQLKLF"
     gene            3514..4341
                     /locus_tag="NBI57_001824"
     CDS             3514..4341
                     /locus_tag="NBI57_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HCD1822759.1"
                     /translation="MLLTITLLVLIGGLSAIIGSIVGIGGGIIIVPTMVYLGVEHGLL
                     HNITTQVAIGTSSVILIVTGLSSSLGYLKTKQVDIKNGSIFLFGLLPGSLLGSFISRY
                     LTFESFNLYFGIFLIFVAILLMVRNKIKPFKIFDKPKYEKTYVDAKGKTYHYSVPPLF
                     AFITTFLIGILTGLFGIGGGALMTPLMLIVFRFPPHVAVGTSMMMIFFSSVMSSIGHI
                     AQGHVAWGYAIILIISSYFGAKIGVKVNQSIKSDTVVTLLRTVMLLMGIYLIIRALI"
     gene            4368..5687
                     /locus_tag="NBI57_001825"
     CDS             4368..5687
                     /locus_tag="NBI57_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HCD1822760.1"
                     /translation="MRLTIYHTNDIHSHLHEYERIKVYMAEQRPRLNHPSLYVDLGDH
                     VDLSAPITEATLGKKNVALLNEAKCDVATIGNNEGMTISHEALNHLYDEAKFIVTCSN
                     VIDESGHLPNNIVSSYIKDMDGVKILFVAATAPFTPFYRALDWIVTDPLESIKEEIKH
                     QRGKFDVIIVLSHCGIFFDETLCQELPEIDVIFGSHTHHYFEHGEINNGVLMAAAGKY
                     GNYLGEVNLTFEAHKVVHKTAKIIPLETLPEVETSFEEEGKTLMSNSVIQHPVVLKRS
                     MNHITEAAYLLAQSVCEYTHAQCAIINAGLLVKDIVKDEVTEYDIHQMLPHPINMVRV
                     RLSGVKLKEIITKSNKQEYMYEHAQGLGFRGNIFGGYILYNLGYIHSTGRYYLNGEEI
                     EDDKEYVLGTIDMYTFGRYFPTLKELPKEYLMPEFLRDIFKEKLLEY"
     gene            5771..6688
                     /gene="lipA"
                     /locus_tag="NBI57_001826"
     CDS             5771..6688
                     /gene="lipA"
                     /locus_tag="NBI57_001826"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HCD1822761.1"
                     /translation="MATKNEEILRKPDWLKIKLNTNENYTGLKKMMREKNLNTVCEEA
                     KCPNIHECWGARRTATFMILGAVCTRACRFCAVKTGLPNELDLNEPERVAESVELMNL
                     KHVVITAVARDDLRDAGSNVYAETVRKVRERNPFTTIEILPSDMGGDYDALETLMASR
                     PDILNHNIETVRRLTPRVRARATYDRTLEFLRRSKELQPDIPTKSSIMVGLGETIEEI
                     YETMDDLRANDVDILTIGQYLQPSRKHLKVQKYYTPLEFGKLRKVAMDKGFKHCQAGP
                     LVRSSYHADEQVNEAAKEKQRQGEAQLNS"
     gene            6818..7201
                     /locus_tag="NBI57_001827"
     CDS             6818..7201
                     /locus_tag="NBI57_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HCD1822762.1"
                     /translation="MIKVDQHYFELIENYRECFNEEQFIARYSDILDKYDYIVGDYGY
                     DQLRLKGFYKDSNKKAEMSKRFSNIQDYIFEYCNFGCPYFVLRHLSKQEVKKLIEEVH
                     PSDVIDDDNKLQDVKIKPTIQDTEH"
BASE COUNT         2628 a         1050 c         1394 g         2212 t
ORIGIN      
        1 taaagtaact atgattaaaa atgcatatta attatcatta ttaagtctat tatatataat
       61 gaattttaac tggtttatta aacgagaacg tcgggaatta agtaactaca ataaaaataa
      121 gatatgacaa taaggagact acacgcgtga tcattgccat aattatattg atatttattt
      181 cgtttttctt ttcaggaagc gagacggcat taacggctgc caataaaaca aaatttaaaa
      241 ctgaagctga caaaggtgat aaaaaagcaa aaggcattgt aaagttactt gaaaaaccaa
      301 gtgagtttat tacaacgatt ctaattggga ataatgtcgc gaatatttta ttaccaacac
      361 ttgttacaat tatggcttta cgttggggga ttagcgttgg tattgcatca gctgttttaa
      421 cagttgttat cattttgatt tccgaagtga ttcccaagtc tgtcgctgca acatttccag
      481 ataaaataac aaggcttgta tatccaatta ttaatatttg tgtcattgtg tttcgcccta
      541 tcacattact tttaaataag ttgacggaca gtattaatcg aagtttatct aagggtcaac
      601 ctcaagaaca tcaattttca aaagaagaat ttaaaacaat gttagcaatt gctggacatg
      661 aaggtgcttt aaatgaaatt gagacgagta ggttggaagg tgtcattaat tttgaaaatt
      721 taaaagtaaa agatgtagat acaacaccta gaattaatgt gacggcattt gcttcaaatg
      781 cgacatacga agaagtttat gaaacggtta tgaataagcc atacactaga tatccagtgt
      841 acgagggaga tattgataac attattgggg tgtttcattc taaatatctg ttggcttgga
      901 gtaataaaaa agaagatcaa attacaaact attcagctaa gccattattt gtgaatgaac
      961 acaataaagc tgaatgggta ttacgtaaga tgactatttc tagaaaacat ttagcaattg
     1021 tgttggacga atttggtggt accgaagcga tagtgtcaca tgaagactta attgaagaat
     1081 tattaggtat ggaaattgaa gatgagatgg ataaaaagga aaaagaaaaa ctttctcaac
     1141 agcaaattca atttcaacaa cggaaaaatc gcaacgtatc tatataagga gcgaacagct
     1201 atgtggaata agaatcgact tactcaaatg ttaagtattg aatatccaat tatacaagca
     1261 ggtatggcag gaagtacgac accgaaatta gttgcatcag taagtaacag tggtgggtta
     1321 ggcacaatag gcgcaggtta ctttaatacg cagcaattgg aagatgaaat agattatgta
     1381 cgccaattaa cgtcaaattc ttttggcgta aatgtctttg taccaagtca acaatcatat
     1441 accagtagtc aaattgaaaa tatgaatgca tggttaaaac cttatcgacg cgcattacat
     1501 ttagaagagc cggttgtaaa aattatcgaa gaacaacaat ttaagtgtca tattgatacg
     1561 ataattaaaa agcaagtgcc tgtatgttgt tttacttttg gaattccaaa tgaatctatt
     1621 atagaaagat tgaaagaagc aaacattaag ctaataggta cagcaacaag tgttgatgaa
     1681 gctattgcga atgaaaaagc gggtatggat gctatcgttg ctcaaggtag tgaagcaggt
     1741 ggacatcgtg gttcattttt aaaacctaaa aatcaattac ctatggttgg aacaatatct
     1801 ttagtgccac aaattgtaga tgtcgtttca attccggtca ttgccgctgg tggaattatg
     1861 gacggtagag gagttttggc aagtattgtc ttaggtgcag aaggggtaca aatgggcact
     1921 gcatttttaa catcacaaga tagtaatgca tcagaactac tacgagacgc aattataaat
     1981 agtaaagaaa cagatacagt cgttacaaaa gcgtttagtg gaaagcttgc acgcggtatc
     2041 aacaataggt ttatcgaaga aatgtcccaa tacgaaggcg atatcccaga ttatccaata
     2101 caaaatgagc taacaagtag cataagaaaa gccgcagcaa acatcggcga caaagagtta
     2161 acacatatgt ggagtggaca aagcccgcga ctagcaacaa cgcatcccgc caacaccatc
     2221 atgtccaata taatcaatca aattaatcaa atcatgcaat ataaataatc gaccgcaatc
     2281 cacaaaagca caagcactcc caaacattat tttagtgctt gccatttttg tggattgcgt
     2341 ttctatttta ccaatctagt caaacgaaat tatcaaacta aagttcgctg aaatgattat
     2401 agtcaaacga aaacatcaaa ctaaagttcg ctgaaatgat tatgatcaaa cgaaattatc
     2461 gagctgaagg tcaccgaaat gattataatt aaacgaaatt atcgagctaa agttcactga
     2521 aatgattata atcaaacgaa aacatcaagc tgaagatcgc cgaaagattt taatcaagcg
     2581 aaaacatcaa attaaagttc gctgaaatga ttatgataaa agttatatgg tatgatgaca
     2641 ttggtgatat atatgataaa catcggatta acaggttggg gtgatcacta ttcattatat
     2701 gaagatttag aacgccaaac cgataaactt aaaacatatg ctggacattt tccggttgtc
     2761 gaattagatg cgacatacta tgcgatacaa ccggaaagaa atatattgaa atggataaaa
     2821 gaaacgcctg atacatttga atttgtggtc aaaattcatc aagcactcac attgcatgca
     2881 gactacaaaa catttgcaga tacaaggcaa gaactatttg atcaatttaa gaatatgtta
     2941 gagcccttac atacacagaa aaaattagca atggtattgg ttcaatttcc gccatggttt
     3001 gactgcaatg cacaaaatat caaatatatt ttgtatgtaa gacagcaatt acaagcattt
     3061 ccaatgtgtg tagaatttag gcatcaatca tggtttagtg atgcatttaa agaacaaaca
     3121 ttggcatttt taacagaaca tcaaatcatt catgcagtag ttgatgaacc acaagtgaaa
     3181 gatggcagtg tacctttagt caatcgaatc acaaatgaaa ttgcgtttgt acgttatcat
     3241 ggacgtaatc attacggttg gactaagaaa gatatgtcag atcaagaatg gcgagatgta
     3301 cgctatttat atgattataa tgagcaagaa ttaatagact tggcacaaaa ggcacaaata
     3361 ttagcacaaa aagctaagaa agtttacgtc atatttaaca ataattctgg tggtcatgca
     3421 gcaaataatg ccaaaacata tcagcgatta ttgaatatag aatatgaagg gttagcacca
     3481 caacaattaa aattatttta agaggcgacg actatgttat taacaattac attattagtt
     3541 ttaatcggag gtttgtcagc gattataggg tctatcgtag gcattggagg cggtattatt
     3601 atcgttccaa caatggttta cctcggtgtt gaacatggat tactacataa tattacaaca
     3661 caagtagcga tagggacgtc ttcagtcatt ctaattgtga caggactttc ttcatcactt
     3721 ggatatttaa aaacaaaaca agttgatatt aaaaatggtt ccatcttttt atttggacta
     3781 ttaccaggtt cattgcttgg gtccttcatt agtagatatt taacatttga gtcatttaat
     3841 ttatattttg gtatcttttt aattttcgta gccattttat taatggtaag aaataagatt
     3901 aaaccgttta aaattttcga taaacccaag tatgaaaaga cttatgtaga cgctaaaggt
     3961 aaaacatatc attatagtgt gccaccattg tttgctttta ttacaacgtt tttaattggt
     4021 atattgacag gtttatttgg tattggaggt ggcgcactaa tgacgccact aatgcttatt
     4081 gtatttagat ttccacctca tgtagctgtt ggaacaagta tgatgatgat tttcttttca
     4141 agtgtcatga gttctatagg gcacattgct caaggtcacg tagcttgggg ttatgcaatc
     4201 attttaatta tttctagtta ttttggtgcg aaaatcggtg tcaaagtgaa tcaatcaatt
     4261 aagtcagata cggtagtaac attattgaga acagtaatgt tgttaatggg tatatattta
     4321 attattcgag cgttgattta atacaacttt aaaaggagga cgtcaatttg aggcttacaa
     4381 tttatcatac gaacgatatt catagtcatt tacatgaata cgaacgcatt aaagtatata
     4441 tggcagaaca acggccacga cttaatcatc cttctttata tgttgatcta ggtgatcatg
     4501 tagatttatc cgcacctata actgaagcaa ctttaggtaa aaagaatgtg gcattactaa
     4561 atgaagcaaa atgtgatgtt gcaacaatcg gtaataatga agggatgacc atttcacacg
     4621 aagctttaaa tcacctttac gacgaagcaa aatttatagt gacatgtagc aatgttatag
     4681 atgaatcagg tcatttacca aataatatcg tttcttctta tattaaggat atggacggtg
     4741 tgaaaatact attcgttgca gcgacagcac cttttacccc attttatcgt gcactagatt
     4801 ggattgttac cgatccactt gaatctataa aagaagaaat taaacatcaa cgaggtaaat
     4861 ttgatgtaat aatcgtgcta agtcattgtg gcattttctt cgatgaaaca ttatgccaag
     4921 aattgcctga aattgatgtc atttttggta gtcatacgca tcattatttt gaacatggtg
     4981 agatcaataa tggtgtactg atggcggcag ctggaaagta tggtaattat cttggagagg
     5041 ttaatttaac ttttgaggca cataaagtag tacataaaac tgcaaagatt attcctttag
     5101 aaacattacc tgaagttgaa acttcatttg aagaagaagg aaaaacgtta atgtccaatt
     5161 cagtaattca acatccagta gtgcttaagc gtagtatgaa tcacataact gaagctgcat
     5221 acttattagc tcaaagtgtt tgtgagtata cacatgcaca atgtgccatc atcaatgctg
     5281 gcttactcgt taaagatatt gtaaaagatg aagtgacaga atatgacatt catcaaatgt
     5341 taccgcatcc gattaatatg gtaagggtta gactttctgg tgtgaaatta aaagagatta
     5401 taactaaaag taataaacaa gaatatatgt atgaacatgc acaaggtttg ggtttcagag
     5461 ggaatatatt tggaggatat attctttata atttagggta cattcattct acagggcgtt
     5521 actatctgaa tggagaagaa atcgaagacg acaaagaata tgtattaggt acgatagaca
     5581 tgtatacgtt cggtcgttat ttcccaacac tgaaagaatt accaaaagag tatttaatgc
     5641 cagagttttt aagagatata tttaaagaaa aattattgga atattaaaaa gtaagattat
     5701 tggattttca tttgtcatga atttcgatat aatgtttaaa gatacactta acaggagggt
     5761 atgtgttgtt atggcgacaa aaaacgagga aatattacgt aaaccggatt ggttgaaaat
     5821 aaaattaaat accaacgaaa actatacagg acttaagaag atgatgaggg aaaaaaatct
     5881 taatactgta tgtgaagaag ctaaatgtcc taatatacat gaatgttggg gtgcacgtcg
     5941 tacagcgaca tttatgattt taggtgccgt atgtacaaga gcttgtcgtt tttgtgcggt
     6001 taagacaggt ttacctaatg aacttgattt aaatgagcct gaacgtgtag ctgaatcagt
     6061 tgaattaatg aatttgaaac acgttgttat cactgctgtt gcgcgtgatg atttaagaga
     6121 tgctggttca aatgtttatg ctgagacagt acgtaaagtt agagaaagaa atccatttac
     6181 aacgattgaa attttaccat cagatatggg cggggactat gatgcgttag aaacattaat
     6241 ggcgtcaaga cctgacattt taaaccataa tattgaaact gttcgtcgct taacaccgag
     6301 agttcgtgcg cgtgcgactt acgacagaac attagagttt ttacgtcgtt caaaagaatt
     6361 acaaccagat atcccaacta aatcaagtat tatggttgga ttaggtgaaa ctatagaaga
     6421 aatttatgaa acgatggatg atttacgtgc gaatgatgta gatattttaa cgattggtca
     6481 atatttacaa ccttcacgta aacatttaaa ggttcaaaaa tattacacgc ctttagagtt
     6541 tggtaaatta agaaaagtgg caatggataa agggtttaaa cattgccaag ctggaccttt
     6601 agtacgtagt tcttatcatg cggatgagca agtaaatgaa gctgctaaag aaaagcaacg
     6661 ccaaggtgag gcacagttaa atagttaata tttaacaatt aataaggcat aaaggcttag
     6721 tttgtacaaa acgaacgtgt catagaagta atcattattt ttatgaaaca ctaaaagtat
     6781 aaactgagcc tttcattgtc atagataggt gaagaatttg ataaaagtag atcaacatta
     6841 ctttgaatta atagaaaatt atcgcgaatg ttttaatgaa gaacaattta ttgctaggta
     6901 ttcagatatt ttagacaaat atgattacat tgttggtgac tatggttacg atcaattacg
     6961 attaaaaggc ttttacaaag attctaataa aaaagcagag atgagtaaac gtttttcaaa
     7021 tattcaagat tacatatttg aatattgtaa ctttggttgt ccttactttg tattaagaca
     7081 tttgtctaaa caagaggtta aaaagttaat cgaagaagtt catccgtctg atgtgataga
     7141 tgacgacaat aaacttcaag atgtgaagat taagccaacc attcaagata ctgaacatta
     7201 ataaaaccct tagctagatt gaaaatggga atcatgcaat tcaagcatgg acctgtaatc
     7261 tagttagggg tttttatctt taat
//
LOCUS       DAHHSC010000117         7263 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.118, whole genome shotgun sequence.
ACCESSION   DAHHSC010000117 DAHHSC010000000
VERSION     DAHHSC010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 7263)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7263)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7263
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.118"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            60..2849
                     /locus_tag="NBI57_001828"
     CDS             60..2849
                     /locus_tag="NBI57_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HCD1822763.1"
                     /translation="MAYQSEYALENEMMNQLEQLGYERVTIRDNKQLLDNFRTILNER
                     HADKLEGNPLTDKEFQRLLTMIDGKSIFESARILRDKLPLRRDDESEIYLSFLDKKSW
                     CKNKFQVTNQVSVEDTYKARYDVTILINGLPLVQVELKRRGIDINEAFNQVKRYRKQN
                     YTGLFRYIQMFIISNGVETRYFSNNDSELLKSHMFYWSDKQNNRINTLQSFAESFMRP
                     CQLAKMISRYMIINETDRILMAMRPYQVYAVEALIQQATETGNNGYVWHTTGSGKTLT
                     SFKASQILSQQDDIKKVIFLVDRKDLDSQTEEEFNKFAKGAVDKTFNTSQLVRQLNDK
                     SLPLIVTTIQKMAKAIQGNAHLLEQYKTNKVVFIIDECHRSQFGDMHRLVKQHFKNAQ
                     YFGFTGTPRFPENSSQDGRTTADIFGRCLHTYLIRDAIHDGNVLGFSVDYINTFKNKA
                     LKAEDNSMVEAIDTEEVWLADKRVELVTRHIINNHDKYTRNRQYSSIFTVQSIHALIK
                     YYETFKRLNKKLEQPLTIAGIFTFKPNEDDRDGEVPYHSREKLEIMISDYNKKFETNF
                     STDTTNEYFNHISKNVKKGVKDSKIDILIVVNMFLTGFDSKVLNTLYVDKNLMYHDLI
                     QAYSRTNRVEKESKPFGKIVNYRDLKKETDDALRVFSQTNDTDTILMRSYEEYKKEFM
                     DAYRELKMIVPTPHMVDDIQDEEELKRFVEAYRLLAKIILRLKAFDEFEFTIDEIGMD
                     EQENEDYKSKYLAVYDQVKRATAEKNKVSILNDIDFEIEMMRNDTINVNYIMNILRQI
                     DLEDKAEQRRNQEQIRRILDHADDPTLRLKRDLIREFIDNVVPSLNKDDDIDQEYVNF
                     ESIKKEAEFKGFAGERSIDEQALKTISNDYQYSGVVNPHHLKKMIGDLPLKEKRKARK
                     AIESFVAETTEKYGV"
     gene            complement(2996..3595)
                     /locus_tag="NBI57_001829"
     CDS             complement(2996..3595)
                     /locus_tag="NBI57_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020808034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1822764.1"
                     /translation="MSLSKLPLFNHFANIRIQPKLIIKLFLTLIIIVTYTFVSGLSVD
                     YKKLLSEAGLSETSLAQIKIFSQISIFFSRVFSVLFTFLIILVISRIMRSDANTKSIF
                     SATLSYILFTNAISLIILTIQISAGLSLIDYSITSLNIFAKGNRILGTFDLQFFVQAY
                     ILTVMLIGTSKLSIRASTIWGIIYLILIITFSLISISIQ"
     gene            3721..4833
                     /locus_tag="NBI57_001830"
     CDS             3721..4833
                     /locus_tag="NBI57_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001253191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HCD1822765.1"
                     /translation="MRRKRKWLIVICVLLVLITISQILKHFNYGTESKEAYNTYTVEK
                     ERQLDLQGKAYPREVKTYYKNNQVGTYLGVQVADGQTVKKGDMLINYKINSNKRQQLV
                     DKINDTQHIITEVYLNITKGLNNREIQKKLIEYKQTLYEAQQQLYQYDSQNRDSMYAS
                     FDGKIMIKNIDNDSDNQPILYLISKESHIKTSVTEFDLNKVKKGDKVNLSITSTGKTG
                     AGIIKQISELPISYEENLSLHENDALHLPESNNDGELANSKSISASPIFKSNNNSELS
                     KYGVIIDDLSLPIRAGYSLEIKVPLNAIKIPKSVLTKGNNVFIVNKNSIVEKRNIRIK
                     RINGDIIVEKGLKPGDKLIKQPKSTMNDGDKVEISS"
     gene            4830..5510
                     /locus_tag="NBI57_001831"
     CDS             4830..5510
                     /locus_tag="NBI57_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000571405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCD1822766.1"
                     /translation="MIELKNVYRTFKNGDKNIRVLKDINIHINAGEFIAIMGPSGSGK
                     STLINILGFIDREYHGEYLFEGSNYQTQSDNQLANIRNKTVGFVFQNFKLIHNNTILE
                     NISMPLVYTNMNTIERKNKVMTVLSAVGLDGKGDFTLSKLSGGQQQRVAIARAIINNP
                     KFIIADEPTGALDSDTSKIIMDIFTSLNRDNHTTIILVTHDRKVAEKADRIIHILDGR
                     IQHEEVIK"
     gene            5507..6685
                     /locus_tag="NBI57_001832"
     CDS             5507..6685
                     /locus_tag="NBI57_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001233395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCD1822767.1"
                     /translation="MRKVLNIFTIAFKSILKNKGRNIFTMIGIIIGISSVITIMSLGN
                     GFKKTAADQFSDAGAGKQEALISFTFKVDEKIKKYPFNQRDIELVNQVDGVLDAKLKE
                     NKEEGIEATITNVQKKSDIFIIKKQNLHSFKVGRGFDKEDNELRKKIVVINDQVAKTV
                     FNNNAIGKSLYIEGQGFEVIGITDKLYSDSSTVIMPENTFNYYMGHLHQGLPTLQIII
                     EDGYNKKTVVKKVESLLNKKGSGSVLGEYTYTDTEEIIKSIDKIFDSITYFVAAVAGI
                     SLFIAGIGVMNVMYISVAERTEEIAIRRAFGAKSRDIELQFLIESILICVTSGFIGLI
                     LGVVFATIIDVLTPDYIKSVVSLSSVIIAVSVSILIGLLFGWIPARAASKKELIDIIK
                     "
     gene            6892..7113
                     /locus_tag="NBI57_001833"
     CDS             6892..7113
                     /locus_tag="NBI57_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000915920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative cyclic bacteriocin"
                     /protein_id="HCD1822768.1"
                     /translation="MLKLHKKIAWTGIKGSAITTFITALYNGSSVWNALAVAGIAFGG
                     GAGTAVAALGRATVMRFIKRWGVRKTAAW"
BASE COUNT         2776 a          916 c         1351 g         2220 t
ORIGIN      
        1 ttttcattaa agtttatgat atatttagag caatagaaag tgtatggaag gggatatgaa
       61 tggcatacca aagtgaatac gcattagaaa atgaaatgat gaatcaactt gaacaattgg
      121 gttacgaaag agtaacgata cgtgataata agcaattgct tgataatttt agaacgattt
      181 taaatgagcg tcatgcggac aaattagaag gcaatccctt aacagataaa gaatttcaac
      241 gtctgttaac gatgattgat gggaaaagta ttttcgagag tgcccgtatt ttacgtgata
      301 aattaccact tagacgtgat gatgagtctg agatttattt gtcgttttta gataagaaaa
      361 gttggtgtaa aaataagttt caagtgacga atcaagtatc tgtcgaggat acatataaag
      421 cacgttatga tgtaacgata ttaatcaacg gactacccct tgtccaagtt gaattgaaac
      481 gtcgaggtat tgatattaat gaggcgttta accaagtaaa acgttaccgc aaacaaaatt
      541 acacaggctt attccgctac atacaaatgt ttatcattag taatggtgtt gaaacgcgat
      601 acttttctaa taatgatagc gaactattga agagtcacat gttttattgg agtgataaac
      661 agaataaccg aatcaataca ttacaatcat ttgctgagtc gtttatgaga ccctgtcaat
      721 tagctaagat gatatcgcgc tatatgatta ttaatgaaac agatagaata ctgatggcaa
      781 tgcgtccgta tcaagtgtat gcggtagaag cacttattca acaagcgact gagacaggga
      841 ataatggata tgtatggcat acaactggaa gtggtaagac attgacttct tttaaagcga
      901 gtcagatttt atcacagcaa gatgacatta agaaagttat ctttttggtt gaccgtaaag
      961 acttggatag tcaaacagaa gaggaattta ataaatttgc taagggtgct gtagacaaaa
     1021 cttttaatac ctcgcaactg gtacgccaac taaatgataa aagtttgcca cttattgtaa
     1081 cgacgattca aaaaatggct aaagcgattc aagggaatgc ccatttatta gaacagtata
     1141 aaacgaataa agttgtattt attattgatg agtgtcatcg cagtcaattt ggtgacatgc
     1201 atcgtctagt taaacaacat ttcaaaaatg cccaatactt tggattcact ggtacgccac
     1261 gttttccaga aaatagtagt caagatggta gaacaactgc agatattttc ggtagatgct
     1321 tacatacgta tttaattaga gatgccattc atgatggtaa tgtacttggt ttctcagttg
     1381 actatattaa tacttttaaa aataaagctt taaaagcaga agataacagc atggttgaag
     1441 caattgatac ggaagaagta tggttagcgg ataaacgtgt ggaattagta acacgacata
     1501 tcatcaataa tcatgataaa tatacacgta atcgtcaata ttcaagtata tttacagtcc
     1561 aaagtattca cgcgcttatt aaatattatg agacatttaa gcgacttaac aaaaagttgg
     1621 aacaaccgtt aacgatagct ggtatattta cgtttaaacc taatgaagat gatcgtgatg
     1681 gtgaagtgcc atatcattca cgtgaaaaat tagagataat gattagtgat tataataaaa
     1741 agttcgagac gaatttttca acagacacaa ctaatgagta ttttaatcat atttcaaaaa
     1801 acgttaaaaa gggcgttaaa gatagtaaaa ttgatatctt aatcgttgtt aatatgttct
     1861 taactggttt tgatagtaaa gtactgaaca ctttatatgt tgataagaat ttaatgtatc
     1921 atgatttaat tcaagcgtat tcacgtacaa atagggttga aaaagaatca aagccatttg
     1981 gtaaaattgt aaactatcgt gacttgaaaa aagagacaga cgatgcactg agagtattct
     2041 cacaaacaaa tgatacggat acaattttaa tgcgcagtta tgaagagtat aaaaaagaat
     2101 ttatggacgc ttatcgtgag cttaaaatga ttgtgccgac accacacatg gttgatgaca
     2161 ttcaagatga agaagagcta aagcgctttg ttgaagctta tcgtttatta gctaaaataa
     2221 tattacgttt aaaagcattt gacgagtttg agtttacaat tgatgaaatt ggaatggatg
     2281 aacaagagaa tgaagactat aaaagtaaat atttagctgt gtacgatcaa gtaaaaagag
     2341 cgacggctga gaaaaataaa gtatccattt taaatgatat tgatttcgaa atagaaatga
     2401 tgcgtaatga tacgattaat gtgaattata ttatgaatat attgagacaa attgatcttg
     2461 aagacaaagc ggaacaacgt cgtaaccaag aacaaattag acgcatttta gatcatgcag
     2521 atgatccgac attgaggtta aaacgagatc taattagaga attcatcgac aatgttgtac
     2581 cttctttaaa taaggatgat gatatcgatc aagaatatgt taatttcgaa agtattaaaa
     2641 aagaagcgga gttcaaagga tttgctggag agagatctat cgatgaacaa gccctaaaaa
     2701 caatttcaaa tgactaccag tatagtggtg ttgtaaaccc acatcacctt aaaaaaatga
     2761 ttggtgattt gccattgaaa gaaaagcgta aagcaagaaa agccattgaa tctttcgtgg
     2821 cagaaacaac tgaaaaatac ggtgtgtaat gattcagccc cctcgctaga ttagtgtagg
     2881 gggcattatt atttcattag aaaaggctca tttgttaaat tcatcttatc aagaatataa
     2941 cgaatgttcc ttgttttaaa cttattaatt aatagagaca ttttatctga aaatatcact
     3001 gaatgctaat actaatcaaa gaaaaagtga taattaaaat aagatatata ataccccaaa
     3061 tagtggatgc tcgtatagaa agtttcgacg taccaattag catgactgta agtatgtatg
     3121 cttgtacaaa aaattgtaag tcgaaagtac ccagaatgcg atttccttta gcaaaaatat
     3181 ttaaactagt aatagaatag tcaattaatg ataaaccggc acttatttgg atggtaagta
     3241 taattaaact aatagcattt gtaaacagta tgtagcttaa cgttgcagag aatatagatt
     3301 ttgtattagc atctgagcgc ataattctag agataactag aatgattaaa aatgtaaata
     3361 atacgctgaa aactcttgag aaaaaaatac taatttgact aaaaattttt atttgtgcta
     3421 atgatgtctc tgagagtcct gcttcactca ataacttttt atagtccaca gatagtccac
     3481 taacaaatgt atatgtaaca attattatca aagtcagaaa cagcttaatg attaactttg
     3541 gttgaatgcg gatatttgca aagtgattaa acaaaggtag ttttgataat gacatataga
     3601 cctcctaatg atataattct cctatatttt atcataaaaa taatataaaa aatatatttt
     3661 attaaaacta agtataaaaa atggtttgaa gagtatttaa catttatagg aggacataaa
     3721 ttgaggcgta aaaggaaatg gctaattgtt atttgtgtat tattagtatt gattacgata
     3781 tcacaaattt tgaaacattt taactatgga actgagagta aagaagcata caatacatat
     3841 acggttgaaa aagaaagaca attagattta caaggtaagg catatccacg tgaagttaaa
     3901 acatattata aaaataatca ggttggaaca tatttgggtg tgcaagtagc ggacgggcaa
     3961 acagtcaaaa aaggagacat gttaattaat tataaaataa atagtaataa acgacagcaa
     4021 cttgttgata agataaatga tactcaacat attattacag aagtttattt aaacattact
     4081 aaaggtttaa acaatcgaga gatacagaag aagttaattg aatataagca gacattatat
     4141 gaagctcaac agcaattgta tcagtatgat agtcaaaata gggatagtat gtatgcatca
     4201 tttgatggca agattatgat caagaatata gataatgaca gtgataatca acctatactg
     4261 tatctcattt caaaggagtc tcatattaaa acgtctgtta cggagtttga tttaaacaaa
     4321 gtcaaaaaag gagacaaagt taacttaagt attacgagta cgggtaaaac aggagcgggg
     4381 attataaaac aaatctctga gttgcctatt agttatgaag agaatttaag tcttcacgaa
     4441 aatgatgccc tgcatttacc agaaagtaac aacgatgggg aattagcaaa ttcaaaatcg
     4501 atttcggcat ctccaatatt taagagtaat aacaactctg aattatcaaa gtatggagtt
     4561 ataattgatg atcttagtct accaatacgt gcaggttatt cgttggaaat taaagtccca
     4621 ttaaatgcga ttaaaatacc taaatcagta ttaacaaaag gcaataatgt ttttattgtt
     4681 aataaaaata gtattgtgga aaaacggaat attcgtataa aaagaatcaa tggtgacatt
     4741 attgtagaaa aggggttaaa acctggagac aagttaatta aacaacctaa aagtacaatg
     4801 aatgatggtg ataaggttga gatttcgtca tgatagaatt aaagaatgtt tataggactt
     4861 ttaaaaacgg tgataaaaat attcgagttt taaaagacat taatatacat atcaatgcag
     4921 gtgaattcat agcgattatg ggaccatcag gctcagggaa gagcacactg attaatatct
     4981 taggatttat agatcgagag tatcatggtg aatatttgtt tgaaggaagt aattatcaga
     5041 cacaatctga taatcaattg gcaaacataa gaaataaaac agttggattt gtttttcaaa
     5101 attttaaact aattcataat aatactattt tagaaaatat aagtatgcca ctggtatata
     5161 caaatatgaa tacgatagaa cgaaagaata aagttatgac tgtgctcagt gctgtaggcc
     5221 tagatggcaa aggtgatttt actcttagta aactatcagg cggtcaacaa caacgtgttg
     5281 caattgcgcg agccattata aataatccaa agtttattat tgcagatgag ccaacgggtg
     5341 ctttggattc agatacatct aaaattatta tggacatatt tacatcatta aaccgagata
     5401 atcatactac aattatttta gtcacacatg atcgtaaagt agcagaaaaa gctgatcgaa
     5461 taattcatat tttggatggt cgtatacaac atgaagaggt gattaaatga ggaaagtttt
     5521 aaatattttt acaatagctt ttaaatcaat attgaaaaat aaaggtagaa atatatttac
     5581 aatgataggt ataatcatcg gtatttcttc tgttattacg ataatgtctt tgggaaatgg
     5641 ctttaaaaaa actgctgctg atcaattttc agatgctggc gccggaaaac aagaagcgtt
     5701 gattagcttt acttttaaag ttgatgagaa aatcaaaaag tatcctttta atcaaagaga
     5761 tatagagttg gtaaatcaag ttgatggcgt actagatgcg aagctaaagg aaaataaaga
     5821 agaaggaatt gaagccacaa taactaatgt ccagaaaaaa agtgacatct tcattataaa
     5881 aaaacaaaat ttacactctt ttaaagtagg tagagggttt gataaagagg ataatgaatt
     5941 acgtaagaaa atagtggtta taaatgatca agtagcaaaa actgtattca ataataatgc
     6001 tattggtaaa tcgctatata ttgagggaca gggatttgaa gtcataggaa ttacggataa
     6061 actatattct gactcatcta ccgttataat gcctgaaaat acattcaact actatatggg
     6121 acacttacat caaggtctgc ctaccttaca gataattatt gaagatggtt acaataaaaa
     6181 aactgtagtt aaaaaagtag aatcactatt aaataaaaaa ggatcgggct ctgttttagg
     6241 tgagtataca tatacagaca ctgaagaaat tatcaaaagt attgataaaa tttttgatag
     6301 tattacttat tttgtagcag ctgttgccgg tatttcactt tttattgctg gtattggagt
     6361 tatgaatgtc atgtatatat ctgtagctga aagaacagaa gaaattgcga tacgacgtgc
     6421 atttggtgca aaaagtcgag atattgaact acaattttta atagagagca ttttaatatg
     6481 tgtgacaagt ggttttattg gacttatttt aggtgttgtg tttgcaacaa taattgatgt
     6541 attaacacca gattatatta aaagtgtagt aagtttgagt tctgtcataa ttgcggttag
     6601 tgtgtcgata ttaattggac ttctttttgg atggatacca gctcgcgcag catctaagaa
     6661 agagctcata gatattataa aatagtaaat ttaaaatggt aaaagtactg aagactaatt
     6721 atcatattaa actgactatc aacaccaacg atggaaatgc gtattagaat aaaagtaaga
     6781 tatgtattgc aaaatgtaaa ttatatattg aatttacgca aaaaataaca tattactttt
     6841 aagatataaa aaaatattat ataatatttt tgagaaggag gtggaaggcc tatgctaaaa
     6901 ttacataaaa aaatagcttg gacaggaatc aaaggttcag ctataactac atttattact
     6961 gccttatata atggaagtag tgtttggaac gcgctagctg ttgctggtat tgcttttggt
     7021 ggtggcgctg gtactgcggt tgcagctctt ggtcgtgcaa cagtaatgag atttatcaaa
     7081 cgttggggcg tacgaaaaac agctgcttgg taaaagataa tctaattctt tataagagag
     7141 gctttctttt ttgggaaagc ctctttttaa aaaaggagat tcaatgaata attcaatatt
     7201 ttatacctat ttaaaattta atcttagata ttttatccgt agaaaatttg aaattgtaaa
     7261 aag
//
LOCUS       DAHHSC010000118         7226 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.119, whole genome shotgun sequence.
ACCESSION   DAHHSC010000118 DAHHSC010000000
VERSION     DAHHSC010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 7226)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7226)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7226
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.119"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            29..1135
                     /gene="rpoD"
                     /locus_tag="NBI57_001834"
     CDS             29..1135
                     /gene="rpoD"
                     /locus_tag="NBI57_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011275651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HCD1822769.1"
                     /translation="MSDNTVKIKKQTIDPTLTLEDVKKQLIEKGKKEGHLSHEEIAEK
                     LQNFDIDSDQMDDFFDQLNDNDISLVNEKDSSDTDEKLNPSDLSAPPGVKINDPVRMY
                     LKEIGRVNLLSAQEEIELAKRIEQGDEVAKSRLAEANLRLVVSIAKRYVGRGMLFLDL
                     IQEGNMGLIKAVEKFDFNKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETINK
                     LIRVQRQLLQDLGRDPAPEEIGEEMDLPAEKVREILKIAQEPVSLETPIGEEDDSHLG
                     DFIEDQEAQSPSDHAAYELLKEQLEDVLDTLTDREENVLRLRFGLDDGRTRTLEEVGK
                     VFGVTRERIRQIEAKALRKLRHPSRSKRLKDFMD"
     gene            1266..1943
                     /locus_tag="NBI57_001835"
     CDS             1266..1943
                     /locus_tag="NBI57_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N(1))-methyltransferase"
                     /protein_id="HCD1822770.1"
                     /translation="MISLNNRLTTVSRFLKQGTIADIGSDHAYLPIYAIQNHLCECGI
                     AGEVIQGPFQAAVKNVAANHLVERIDVRLGDGLSVIHPEDVIDNITICGMGGPLIAKI
                     LKDGQDKLSQHPRLILQSNIQTENLRQTLQQLNYEIIDEIIMEEKGHIYEIVVAEHST
                     QLIELSSDELKFGPKLLNNKNEYFIKKWQRELEALYHIKSKLNTEQHHQRLAQINDEI
                     AVIERVL"
     gene            1946..3046
                     /locus_tag="NBI57_001836"
     CDS             1946..3046
                     /locus_tag="NBI57_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HCD1822771.1"
                     /translation="MKIADLMTLLDHHVPFSTAESWDNVGLLIGDGDVEVTGVLTALD
                     CTLEVVNEAIEKGYNTIISHHPLIFKGVTSLKANGYGLIIRKLIQHDINLIAMHTNLD
                     VNPYGVNMMLAKAMGLKNISIINNQQDVYYKVQTYIPKDNVGPFKDKLSENGLAQEGN
                     YEYCFFESEGRGQFKPVGEANPTIGQIDKIEDVDEVKIEFMIDAYQKSRAEQLIKQYH
                     PYETPVFDFIEIKQTSLYGLGVMAEVDNQMTLEDFAADIKSKLNIPSVRFVGESNQKI
                     KRIAIIGGSGIGYEYQAVQQGADVFVTGDIKHHDALDAKIHGVNLIDINHYSEYVMKE
                     GLKTLLMNWFNIEKINIDVEASTINTDPFQYI"
     gene            3160..4506
                     /locus_tag="NBI57_001837"
     CDS             3160..4506
                     /locus_tag="NBI57_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HCD1822772.1"
                     /translation="MAKHPFEQFNLESSLIDAVKDLNFEKPTEIQNRIIPRILKRTNL
                     IGQSQTGTGKSHAFLLPLMQLIDSEIKEPQAIVVAPTRELAQQLYDAANHLSQFKAGV
                     SVKVFIGGTDIEKDRQRCNAQPQLIIGTPTRINDLAKTGHLHVHLASYLVIDEADLMI
                     DLGLIEDVDYIAARLEDNANIAVFSATIPQQLQPFLNKYLSHPEYVAVDSKKQNKKNI
                     EFFLIPTKGAAKVEKTLNLIDILNPYLCIIFCNSRDNANDLARSLNEAGIKVGMIHGG
                     LTPRERKQQMKRIRNLEFQYVIASDLASRGIDIEGVSHVINFDVPNDIDFFTHRVGRT
                     GRGNYKGVAITLYSPDEEHNISLIEDRGFVFNTVDIKDGELKEVKAHNQRQARMRKDD
                     HLTNQVKNKVRSKIKNKVKPGYKKKFKQEVEKMKRQERKQFSKQQNRQKRKQNKKG"
     gene            4516..5406
                     /locus_tag="NBI57_001838"
     CDS             4516..5406
                     /locus_tag="NBI57_001838"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HCD1822773.1"
                     /translation="MLLGSHVSMSGKKMLEGSAIEAYEYGETTFMIYTGAPQNTRRKS
                     IEDLNITKGHEVMEKYGLSNIVVHAPYIINIANTTKPETFNLGVDFLQQEIERTQAIG
                     AKDIVLHPGAHVGAGVDAGINKIIEGLNEVLTNDNNVRIALETMAGKGTEIGRSFEEL
                     ARIIDGVHNNERLSVCFDTCHTHDAGYNVKEDFDGVLNEFDKIIGVDRIKVVHVNDSK
                     NDRGAQKDRHENIGFGYIGFDALNYIVHHDSFKDIPKILETPYVGEDKKNKKPPYKLE
                     IEMLKQQQFDPELKNKVMQQ"
     gene            5532..6317
                     /locus_tag="NBI57_001839"
     CDS             5532..6317
                     /locus_tag="NBI57_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HCD1822774.1"
                     /translation="MRGENTMTPVFELKNVNYYYDHKKVLENINIKINKGEFLAIVGP
                     NGAGKSTLLKLILGLLPLQSGEIFVEGIDFKNKKTSIKLSYVSQKANAFNSGFPASVK
                     EVVLSGLTKTKRLFQTFNSKDNEKVIKVLERLNISDLIHKNIAELSGGQQQRVMIARA
                     LISEPAVLVLDEPTNGIDAKHVSEFYNTLDQLKQEGITIILVTHDIGVVADTATEVAC
                     LNKHLHFHGTTDEFKSLDEVEISKIYGHPVRFVDHQHNRECCN"
     gene            6359..7222
                     /locus_tag="NBI57_001840"
     CDS             6359..7222
                     /locus_tag="NBI57_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HCD1822775.1"
                     /translation="MIDALLNFDFMRYSLISGILIGFIAPLIGAFIVVRRLSLIADAL
                     SHVTLGGISFGMFLLTIMPTLVFINPMWFGILFAIVGALLIEKLRTSYTAYQEIAIPI
                     IMSAGIALSAIFISLADGFNQEIVGLLFGSISAVNISDLTTIIVIAIIVVLFITLFYK
                     ELFILSFDEEYSKVIGIHKWIQFLFIVIVAMVISASMRVVGILLVSALITLPIAISMR
                     ITKSFKQLILLSVFLGELSVILGLVLAFYMDISPGGVIVVLLVILLMITMAYQKMRMK
                     FKKGANINEYK"
BASE COUNT         2654 a          945 c         1358 g         2269 t
ORIGIN      
        1 ttcatattta aaatcgggag gccgtttcat gtctgataac acagttaaaa ttaaaaaaca
       61 aacaattgat ccgacattaa cattagaaga tgttaagaag caattaattg aaaaaggtaa
      121 aaaagagggt catttaagtc atgaagaaat tgctgaaaaa cttcagaatt ttgatatcga
      181 ctctgatcaa atggatgatt tctttgatca attaaatgat aatgatattt cactagttaa
      241 tgaaaaagat agttcagata ctgacgagaa actgaatcca agtgatctta gtgcccctcc
      301 aggtgttaaa ataaatgacc cagttcgtat gtaccttaaa gaaattgggc gtgttaactt
      361 attaagtgca caagaagaaa tcgaattagc caaacgtatt gaacaaggtg atgaagtagc
      421 aaaatcaaga cttgcagaag cgaacttacg tttagttgta agtattgcta aaagatacgt
      481 aggtcgtggt atgttattcc ttgatttaat ccaagaaggt aatatgggtc ttattaaagc
      541 tgttgaaaaa tttgacttta acaaaggatt taagttttca acatatgcaa catggtggat
      601 tagacaagca atcactcgtg caattgctga ccaagcacgt acgattcgta tccctgtgca
      661 tatggtagaa acaattaata aattaattcg tgttcaacgt caattattac aggacttagg
      721 tcgagatcca gcaccagaag aaattggtga agaaatggat ttaccagcag aaaaagttcg
      781 tgaaatttta aaaattgcgc aagaacctgt ttcattagaa acaccaattg gtgaagaaga
      841 tgatagtcat ttaggagact ttattgagga tcaggaagca caaagtcctt cagatcatgc
      901 tgcttatgaa ttattaaaag agcaattaga agatgtgctt gatacattaa ctgatagaga
      961 agaaaatgta ttacgattaa gatttggtct tgatgacggc agaacaagaa cacttgaaga
     1021 agttggtaaa gttttcggtg ttacacgtga acgtattcga caaattgaag caaaagcact
     1081 tagaaaatta agacatccaa gtcgtagtaa acgtttgaaa gactttatgg attaatttat
     1141 atcaatttta atgattgata ccaagacatg aataagcgct tatttatttt tagattaaga
     1201 tttaacttga gtgaggttgg tacattgaat aatgttcaac ctcttttttc ataaaggaga
     1261 ataaaatgat ttcgttaaat aaccgattaa cgacggtaag tcgattttta aaacaaggta
     1321 caatcgcgga tattggctca gatcatgctt atttgccaat ctatgcaatt caaaaccatt
     1381 tatgcgaatg cggtattgct ggagaagtta ttcaaggccc ttttcaagct gctgtgaaaa
     1441 atgtagctgc aaatcattta gtagaaagaa ttgatgtacg tttaggggat ggcttaagtg
     1501 ttattcatcc tgaggacgta atcgataata tcacaatttg tggtatgggc ggaccactta
     1561 ttgctaaaat tttaaaagat gggcaagata agttaagtca acatccaagg ttgattttac
     1621 aaagtaatat acaaactgaa aatttaagac aaacattaca acaattgaat tatgaaataa
     1681 ttgatgaaat aataatggaa gaaaaaggcc atatttatga aattgttgta gcagaacatt
     1741 ctacacaatt aatagagcta tcatctgatg aattgaaatt cggaccaaaa ttgctgaaca
     1801 acaaaaatga atattttata aaaaaatggc aaagagagtt agaagcactt tatcatatta
     1861 aatcgaaact caatactgaa caacatcatc aacgtttagc tcaaattaat gacgaaattg
     1921 ctgtaattga aagggtgtta tagtaatgaa aatagctgat ttaatgacat tgttagatca
     1981 tcatgtgcca tttagtactg ctgaatcttg ggataatgta ggattgttaa taggtgatgg
     2041 agatgttgaa gttactggtg ttttaacagc attagactgt acgttggaag tagtaaatga
     2101 agcaatcgaa aaaggttata atactattat tagtcatcat cctctaatct ttaaaggcgt
     2161 aacatcatta aaagctaatg gttatggttt gatcattaga aaactaattc aacatgacat
     2221 taatttaata gcgatgcata caaatttaga tgtaaatccg tatggtgtca atatgatgtt
     2281 ggcgaaggcg atgggtttga agaacatttc aataataaat aatcaacaag atgtatacta
     2341 taaagttcaa acatatatac ctaaggataa tgttggacca tttaaagata agcttagtga
     2401 aaatggatta gcgcaagaag gtaattatga atattgtttc tttgaaagtg aaggaagagg
     2461 gcaattcaaa ccagttggtg aagctaatcc aacaatagga caaattgata aaattgaaga
     2521 tgtagatgaa gttaaaattg aatttatgat agatgcatat caaaagtcaa gggctgagca
     2581 attaattaaa caataccatc catatgaaac accggtattt gattttattg agataaaaca
     2641 aacatccctt tatggacttg gcgttatggc agaagtggat aatcaaatga cattggaaga
     2701 tttcgcagct gatattaaat ctaaattaaa tatcccaagt gtccgttttg ttggtgagtc
     2761 taatcagaaa attaaacgta ttgcaattat tggtggttca ggtattggat atgaatatca
     2821 agctgtccaa caaggcgcag atgtctttgt tacaggtgat attaaacatc atgatgcctt
     2881 agatgctaaa attcatggtg tgaatttaat tgatattaat cattacagcg aatatgtgat
     2941 gaaagaaggt ttaaaaacgt tactaatgaa ttggttcaat atagaaaaaa taaatataga
     3001 tgttgaagca tctacaatta atacagatcc atttcaatat atttaaacta aaatctcggg
     3061 atttctaaat tttgaaattt cgaggtttta atatttttat ttaaaatagc acatttatac
     3121 tttataatag taaagatgaa catataagga ggccaaatca tggcaaaaca tccattcgaa
     3181 caatttaatc tagaatctag tttaattgac gctgtgaaag accttaattt tgaaaaacca
     3241 actgaaattc agaatcgaat tattccaaga atactaaaga gaacaaattt aattggtcaa
     3301 tctcaaacgg gtacagggaa atctcatgca tttttattac cattaatgca gttaattgat
     3361 agtgaaataa aagaaccaca agcaatcgta gttgcaccaa caagagaact tgcacaacaa
     3421 ctatacgatg cagcgaacca tttaagccaa tttaaagctg gtgtttcagt taaagttttt
     3481 attggtggta cagatataga gaaagataga caacgttgta atgcacaacc acaattgatt
     3541 ataggcaccc ctactagaat taatgactta gctaaaacgg gacatttaca tgtgcactta
     3601 gcatcatatt tagttattga tgaagcggat cttatgattg acttaggatt aattgaagat
     3661 gtagattaca ttgctgcaag attggaagat aatgcaaata ttgcggtgtt tagtgctaca
     3721 attccacaac agttacaacc atttttaaat aaatatttaa gtcatccaga atatgtagct
     3781 gtcgacagta aaaaacaaaa taaaaagaac atcgaattct ttttaatacc tactaaaggt
     3841 gcagctaaag ttgaaaaaac tttaaattta attgatatac taaatccata cttatgtatt
     3901 attttctgta atagtagaga taatgcaaat gatttagcac gttcactaaa tgaagctggt
     3961 attaaagttg gtatgattca tggtggttta acgccacgtg aacgtaaaca acaaatgaaa
     4021 cgtatacgta atttagaatt ccaatacgtt attgccagcg atttagcatc tcgtggtatt
     4081 gatattgaag gtgttagtca tgtcatcaat tttgatgtgc caaatgatat tgacttcttt
     4141 acgcatagag ttggacgaac tggtcgtggg aattataaag gtgtagcaat tacgctttat
     4201 agtcctgatg aagaacacaa tatttcatta atagaagatc gcggttttgt attcaatact
     4261 gttgatatta aagatggtga gttaaaagaa gttaaagcgc acaatcagcg tcaagcaaga
     4321 atgcgcaaag atgaccattt aactaatcaa gtgaagaaca aagttcgaag taaaattaaa
     4381 aacaaagtta aaccaggtta taagaagaaa tttaaacaag aagttgaaaa aatgaaacgt
     4441 caagagcgta aacaatttag taagcagcaa aatagacaaa aacgtaagca aaacaaaaaa
     4501 ggttaggtgt gttttatgtt attaggatca catgtttcaa tgagtggtaa aaagatgtta
     4561 gaaggttctg ctatagaagc gtatgaatat ggtgaaacaa catttatgat ttatactggt
     4621 gcacctcaaa acacgcgccg taaaagtatt gaagatttaa atataactaa aggtcatgaa
     4681 gtgatggaaa aatatggctt atctaatatt gttgttcatg caccatacat cattaatatt
     4741 gcaaatacaa ccaaaccaga aacgtttaat cttggtgtag atttcttgca acaagaaatt
     4801 gaaagaacgc aagctatagg cgctaaagat attgtattac atccaggtgc gcatgttgga
     4861 gctggtgttg atgccggaat taataagatt attgaaggat taaatgaagt tttaacgaat
     4921 gacaataatg tacgtattgc gcttgaaacg atggcaggta aaggtacaga gattggtcgc
     4981 tcgtttgaag agttagcacg tattattgat ggagttcata ataacgaacg tttatcagta
     5041 tgttttgata catgtcatac gcatgatgct ggatataatg tgaaagagga ctttgatggc
     5101 gtattaaatg aatttgataa aattattgga gtcgacagaa tcaaagttgt tcatgtcaat
     5161 gattctaaaa atgaccgtgg cgctcaaaaa gaccgacatg aaaatattgg cttcggttac
     5221 attggttttg atgcattaaa ttacattgtg catcatgata gttttaaaga tattccgaaa
     5281 atcttagaaa caccttatgt tggtgaagat aagaaaaata aaaaaccgcc atataaactt
     5341 gaaattgaaa tgttaaaaca acagcaattt gacccagaat taaaaaataa ggttatgcaa
     5401 caataaaatg ataatgctct cttcaagaaa tgcgataaat aaaacttgaa gggggcatat
     5461 ctctttattt tgtctaattt tgagtcgtaa acattactgt ttacatatgt tactaggagt
     5521 tgtatagttg aatgcgaggt gagaatacga tgacaccagt ctttgaattg aaaaatgtca
     5581 attactacta tgatcataaa aaagtgttag aaaatataaa cattaaaata aataaaggtg
     5641 aatttttagc gattgttgga ccaaatggtg ctggtaaatc aacattattg aagttgattc
     5701 tagggttatt acctttacaa agtggtgaga tttttgttga aggtattgat tttaaaaata
     5761 agaaaacatc aattaaatta agctatgtat cacaaaaagc aaatgccttt aattcaggtt
     5821 tcccagcaag tgttaaagaa gttgttttaa gcggattaac aaagacaaaa cgtcttttcc
     5881 aaacatttaa tagcaaagat aatgaaaaag tgattaaagt actagaaaga ctgaatataa
     5941 gtgatttaat tcataaaaat atagcagaat tatcaggtgg tcaacaacaa cgtgtaatga
     6001 ttgcccgagc attgatttca gaacctgcag tattagtact tgatgaacca acgaatggta
     6061 ttgatgcaaa acatgtaagt gaattttata atactttaga tcaattaaaa caagaaggta
     6121 tcaccattat cttagttact catgatatcg gtgttgtagc agatactgct actgaagtag
     6181 catgtttaaa taagcatttg catttccatg gtacaactga tgagtttaaa tcacttgatg
     6241 aagttgaaat ttcaaaaatt tatggacatc ctgtacgttt tgtcgatcat cagcataatc
     6301 gagaatgttg taattaataa agatttgagt ttatttaaaa tggaaaggcg tgtcacaatt
     6361 gatagatgca ttattgaatt ttgattttat gaggtactct ttaattagtg gtatcttgat
     6421 aggttttatt gcgcctctaa tcggtgcttt tatcgttgtt agacgactat cacttatagc
     6481 tgatgctcta agtcatgtaa ctttaggtgg tatatccttc ggtatgtttt tacttactat
     6541 tatgccaaca ctagtattta ttaatccaat gtggtttgga atcttattcg caatagtagg
     6601 tgcgcttcta attgaaaaat taagaacgtc atacactgct taccaagaaa ttgctattcc
     6661 aattataatg agtgctggta tcgccttgag tgcaatcttc atttcattag ctgatgggtt
     6721 caatcaagaa attgttggat tgctatttgg ctcaataagc gcagtgaata taagtgattt
     6781 aacaacgatt attgtcattg ccatcatagt tgttctgttt attacattgt tttataaaga
     6841 attatttata ttgtcatttg atgaggaata tagtaaagtc attggcattc ataaatggat
     6901 tcaattttta tttattgtta ttgtagcgat ggttatttct gcctcaatgc gtgttgtagg
     6961 gatactgtta gtaagtgctt taatcacgtt accaattgca ataagtatgc gaataacaaa
     7021 aagttttaag caattgatat tactaagtgt atttttaggt gaattatcag taatattagg
     7081 cttagtccta gctttctata tggacatttc tccgggtggt gtaattgtag ttctactagt
     7141 tattttactt atgattacaa tggcttatca gaaaatgcga atgaagttta aaaagggagc
     7201 taatatcaat gaatacaaat gatgct
//
LOCUS       DAHHSC010000119         7205 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.120, whole genome shotgun sequence.
ACCESSION   DAHHSC010000119 DAHHSC010000000
VERSION     DAHHSC010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 7205)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7205)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7205
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.120"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            complement(18..1226)
                     /gene="gltS"
                     /locus_tag="NBI57_001841"
     CDS             complement(18..1226)
                     /gene="gltS"
                     /locus_tag="NBI57_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HCD1822776.1"
                     /translation="MIELNAITTLCLACILYLLGTAIVNHVNFLKRICIPAPVIGGLI
                     FAILVAALDSFGMVKIKLDASFIQDFFMLAFFTTIGLGASLKLFKLGGKVLLLYFMFC
                     AIISVIQNIVGVSLAKVLNIKPLLGLTAGSMSMEGGHGNAAAYGKTIQDLGIDSALTA
                     ALAAATLGLVFGGLIGGPVVKYLIKRYNLKPQHSDDTFKDYSQVAYNEHLHSKFNATE
                     VFFIQFTIVVFCMAVGSYFSHLFTAQTGINVPIYVGSLFVAVIVRNISESFNFNIVDL
                     KITNQIGDVALGIFLSLALMSIQLIEIYKLAIPLIIIVLVQVVVMILFAVLILFRGLG
                     KDYDAAVMVGGFIGHGLGATPNAMANLDVITKKYGNSPKAYLVVPIVGAFLIDLIGVI
                     VIMGFIQWFS"
     gene            complement(1559..2167)
                     /locus_tag="NBI57_001842"
     CDS             complement(1559..2167)
                     /locus_tag="NBI57_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HCD1822777.1"
                     /translation="MDFVNNDTRQIAKNLLGVKVIYQDTTQTYTGYIVETEAYLGLND
                     RAAHGYGGKITPKVTSLYKRGGTIYAHVMHTHLLINFVTKSEGIPEGVLIRAIEPEEG
                     LSAMFRNRGKKGYEVTNGPGKWTKAFNIPRAIDGATLNDCRLSIDTKNRKYPKDIIAS
                     PRIGIPNKGDWTHKSLRYTVKGNPFVSRMRKSDCMFPEDTWK"
     gene            2566..3186
                     /locus_tag="NBI57_001843"
     CDS             2566..3186
                     /locus_tag="NBI57_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HCD1822778.1"
                     /translation="MEQLPQVGFMKSFVLFWKNYVNFKGRSRRSEYWYMALWHLILMV
                     PAVCVLIINLFLVFISIAADNETLIVMSILMIILTSVYTFVYSLATFIPNLALCVRRF
                     HDISRTMLFPMIMTVFSIIFYIVLQIIDSYYDSDFTLMPMGLNILLVLLYFVYLGLTI
                     VMIVFLCFDSKPANKYGESPKYPSTIKNQPVTSETPKTPEETDNQL"
     gene            3417..4316
                     /locus_tag="NBI57_001844"
     CDS             3417..4316
                     /locus_tag="NBI57_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCD1822779.1"
                     /translation="MGLVIEHVTKRFGKMTAVNDISLELESGKMLGFLGRNGAGKTTT
                     FRMILGLSEPTEGHITYNGKKLDKTMYNRIGYLPEERGLHGKLTVEEELKYLATLKGM
                     SKTEIQQQISYWLERFDITENRKKRIDSLSKGNQQKIQLLASMLHKPELLILDEPFSG
                     LDPVNVELLKEAVKDLNDWGSTIVYSSHRMEHVEELCDDVCILDKGQLVVSGDINHVR
                     ASNGNKKVVIESETTLPDLTNIRGIIHSENMKQGLQLTIENEDVAKDIYQVVAHQGYV
                     KRFQVVEPSLQDIFIEKVGGKDA"
     gene            4309..5541
                     /locus_tag="NBI57_001845"
     CDS             4309..5541
                     /locus_tag="NBI57_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCD1822780.1"
                     /translation="MHKFWATFTLTYIKKIKAKSFVIFMIIIAALMIGLSNIDKIINM
                     FDDGPDKIGVAAPNEQIYKVFKQQANTFHSDAKFTKVSIEDAEKEVKKHKLDKAYIIK
                     VNQNRTLQGTIISEKRVSHEDSQKVQALLTAIQTNMVAGELNINKEDLQKLQAQSKVD
                     NKVISNDEVDKVSEGQKIFNYALAYGIIFLMFFIVLNYASQIAMEIASEKTSRVIEMI
                     ITSISPIQHIFAKILGVIAVAVTQIILIVLMAVICIFAFDLKDLLQGFNVEMNQLSWQ
                     IIIVGIISSIVGIMAYVLLAAILGSLTSRIEDLNQSLMPLTLLGMIAFYIAIFTINNP
                     DGQLALITSYIPFLAPFQLVVRAQTSGLQIYEVILSSLISIVMVAVLIWIAIKTYKDS
                     VLTFERGLFNSMKRVFKK"
     gene            5525..5710
                     /locus_tag="NBI57_001846"
     CDS             5525..5710
                     /locus_tag="NBI57_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1822781.1"
                     /translation="MYLKNKIDESDTHLSYKMMMKACYSYFFIFSYRIMSKKVYNMNV
                     KTNFLKWECEVRRNVNK"
     gene            5759..6085
                     /locus_tag="NBI57_001847"
     CDS             5759..6085
                     /locus_tag="NBI57_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnfA family protein"
                     /protein_id="HCD1822782.1"
                     /translation="MLYPIFIFILAGLCEIGGGYLIWLWLREGQSSLVGLIGGAILML
                     YGVIATFQSFPSFGRVYAAYGGVFIIMSLIFAMVVDKQMPDKYDVIGAIICIVGVLVM
                     LLPSRA"
     gene            6115..7134
                     /locus_tag="NBI57_001848"
     CDS             6115..7134
                     /locus_tag="NBI57_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HCD1822783.1"
                     /translation="MIVEVEHQRQGVDLIKIDNDETKIIFTNYGARVVSWKYHDNNIV
                     LGNVVEADEFYHSNPFKFGASIGRYSGRIDNAKFKLKGKEYQLEKNNGEHHLHGGCHG
                     LDNKLFDYEIRNEIAQIKVIFKTVLKSADDHFPGDIDVTITHIYDADHQWSIEYEAVA
                     SEDTLFSPMNHVYFNLNRDNNVVDNHRISSNQLDMYVLDERNIVTGDILDLHEVFEDN
                     KIKLSDIFTSQHAQLSQQMTRFGGLDHPFTVGEHKMYVENHEFMLEVDTDMPHVVFFT
                     FNQPDEWDSPFNIYKPHSGFTLETQFLPNDINLYGEKAPSILKAGEVFNSKTSFRIEE
                     KVEND"
BASE COUNT         2552 a         1028 c         1280 g         2345 t
ORIGIN      
        1 atttatgagt ttggtgttta actaaaccat tgaatgaatc ccataatgac tataacacca
       61 attaaatcga ttaagaatgc accaacaata ggtacaacta aatatgcttt aggtgagttt
      121 ccatattttt tagtaataac atctaaattt gccatggcat ttggcgttgc accaagccca
      181 tgcccgataa aaccacctac cattactgca gcatcataat cttttcctaa acctctaaat
      241 aaaattaaaa cagcaaataa aatcataacg acaacttgaa ctaaaacgat aataataaga
      301 ggtatagcaa gtttataaat ttcgattaat tgaatgctca ttagcgcaag agataagaaa
      361 atacctaatg cgacatcgcc aatttgatta gtaattttta aatctacaat attaaaatta
      421 aaactttcag agatatttcg gacgataaca gctacaaata atgagccaac gtaaattgga
      481 acattaatcc ctgtttgagc tgtaaacaaa tgactgaaat aacttccaac tgccatacag
      541 aatacaacga ttgtaaattg aatgaagaat acttcagtgg cattaaattt actatgtaaa
      601 tgttcgttat atgctacttg gctataatct ttaaatgtat cgtcactatg ttgaggcttt
      661 aagttataac gtttgattaa atattttaca actggaccac cgataagccc tccaaataca
      721 agacctaaag ttgcagctgc aagagccgct gtcagtgccg aatcaatacc taaatcttga
      781 attgtcttac cataagcagc agcattacca tgaccgcctt ccatagacat ggaacctgct
      841 gttaatccta gtaacggttt aatatttaat acttttgcta gtgatacacc aactatgttt
      901 tgaatgactg aaatgatagc acaaaacata aagtataata gcaagacttt gccacctaat
      961 ttaaataatt tcaatgatgc accaagaccg attgtcgtaa agaatgctaa catgaagaaa
     1021 tcttgaatga atgaagcatc taatttaatc ttaaccatgc caaatgaatc caaagccgca
     1081 actaaaatag caaagattaa gccgccaatc actggtgctg gtatacaaat gcgtttcaaa
     1141 aaattaacgt gattaacgat agctgtacca agtaagtaaa gtatacaggc taaacataat
     1201 gttgtaattg cattaagttc tatcattttc aaatccccct tgttgaatat ccctctccct
     1261 aaataagctt ttatatgtaa gcgttataaa agggcacttt taacattata catgcaacaa
     1321 atcaatattt ctagtttttg aaaataatat caataatatt aatgcatcta aatttaataa
     1381 ttataaataa ttctattgtt atattttata attaagaaca agttcaaaaa gaggagatga
     1441 atcaaaaaat atgatggaca aaaaaacaat caacccatcc gcatgttggc ggcacgattg
     1501 attgacttat aaggagatag atttcatttt catgatagta atcaatgaaa gatggcattt
     1561 atttccaagt atcttcggga aacatacaat ctgatttacg catgcgagac acaaatggat
     1621 tacctttcac tgtgtaacgt aaagatttat gtgtccaatc acctttattt ggaataccga
     1681 ttcgtggact agcaataata tctttaggat atttacgatt cttagtatca atagacaatc
     1741 tacagtcatt taacgtagcg ccatcgatag cccgtggaat gttaaatgcc ttagtccatt
     1801 ttcctgggcc attcgttacc tcgtagcctt tcttacctct gttacggaac atagcggata
     1861 aaccttcttc tggttcaatt gcgcgtataa gtacgccttc aggtatacct tcagattttg
     1921 ttacaaaatt aatgagtaaa tgcgtatgca tgacatgtgc ataaattgta ccaccacgtt
     1981 tatataatga cgtgacttta ggtgttattt taccgccata gccatgagcc gcacgatcat
     2041 tcaaacctaa gtaagcttcc gtttccacga tgtagcctgt atacgtttga gtggtatcct
     2101 gataaatcac tttgacacct aataagtttt tagcaatttg tcttgtatca ttattaacga
     2161 aatccataat attctcctcg caattatatg attaaattaa ataaaaatga aaaagatatt
     2221 tttattgtgg taaatatttg ataaaatagt attgttaaga attataaaaa tgtaaagtat
     2281 atactttgtt atggaatatt ttatttgcag agattgagac tcttttaaac gtgaacattg
     2341 cagtatgaca tatttttagt atagttaacg aagcgttgac tttcaaatga gtctccatat
     2401 agatgtgtac ttgtagcggg ttttcatttt actagtgatg aaatataaag tccattttcc
     2461 aaagtgaatg acgattgaaa tgaagtggct tgataatgaa tcttgtaaat tataacggta
     2521 acaataatta aaaatataaa tatgattaac gaaagggaga ttacaatgga acaattacct
     2581 caagtaggtt ttatgaaatc attcgtatta ttttggaaaa attatgtaaa ttttaagggg
     2641 agatcaagac gtagtgaata ttggtatatg gcactatggc atttgatact tatggttcca
     2701 gctgtgtgtg tgctaataat aaacctattt ctagtcttta tttctatagc ggcagataac
     2761 gaaacattaa tagttatgag tatattgatg attatcctta catctgtgta tacttttgtc
     2821 tatagtttgg caacgtttat tccaaattta gcgttgtgcg ttcgaagatt tcacgatata
     2881 tcaagaacaa tgttatttcc aatgattatg acagtattca gtattatatt ttatatagta
     2941 ttacagatta tcgatagtta ttatgacagt gattttacat taatgccgat gggtcttaat
     3001 attttgttag tacttcttta ttttgtttat ttaggcctaa caattgttat gatagtattc
     3061 ctatgctttg acagtaagcc ggcaaataaa tatggcgaaa gcccaaaata cccgtctaca
     3121 attaaaaatc aaccagtgac atctgaaaca ccgaaaacac cggaagaaac agataatcaa
     3181 ttgtaatgat ataaatatct cggcacaatg atgctgggat atttttttac aaataaggca
     3241 ataaaggtat gggaaatgcg tatctagcgc taatgatact taacatgcaa agaagatact
     3301 atttaaatat ttcaatttca gaaattttaa aaataaattc actttaaaaa tattgcattt
     3361 atagagaata gaaatgttat attaagataa atacatattt ttttaggagg caaagtatgg
     3421 gacttgtaat tgaacatgtt acgaaacgtt ttggcaagat gacggcagta aatgatattt
     3481 cattagagtt agaatcaggc aaaatgttag gttttctagg aagaaatggt gcaggtaaaa
     3541 caacgacatt tcgtatgatt ttaggcttaa gtgaaccaac tgaaggacac attacgtata
     3601 acggtaaaaa actagataaa acaatgtaca atcgtattgg ttatttaccg gaagaacgcg
     3661 gtttacatgg gaaattgaca gttgaagaag aattgaaata tttggcaact ttaaagggaa
     3721 tgtcaaaaac agaaatacaa cagcaaatat cgtattggct tgagcgtttt gatattactg
     3781 aaaaccgcaa aaaacgaatt gatagtctat caaaagggaa tcaacaaaaa attcagctgt
     3841 tagcaagtat gttacataaa ccagaattac taatattaga tgagcctttc agtggtctag
     3901 acccagttaa tgttgagtta ttaaaagaag cagttaaaga cttaaatgat tggggcagta
     3961 caatcgttta tagttctcac cgtatggagc atgttgaaga attatgtgat gatgtttgta
     4021 ttttagataa aggtcaactt gttgtttctg gtgatatcaa tcatgtacgt gcatctaatg
     4081 gtaacaagaa agttgtcatt gaatcagaaa caacactacc agatttaaca aacattagag
     4141 gcatcattca tagtgaaaac atgaagcaag gtcttcagtt gacgattgag aatgaagacg
     4201 tagccaaaga tatttatcaa gttgtggcac atcaaggtta tgttaaacga ttccaagttg
     4261 tagaaccatc attacaagat atattcattg agaaggttgg tggcaaggat gcataagttt
     4321 tgggcgacat ttactttgac atatatcaaa aaaattaaag ctaaatcatt tgtaattttt
     4381 atgattatta ttgctgcctt aatgattggc ctatctaaca ttgataagat tattaacatg
     4441 tttgatgatg gtccagataa aattggtgta gcagcaccga atgaacaaat ctataaggtt
     4501 ttcaagcaac aggcgaatac attccatagt gatgctaagt ttactaaagt ttctatagaa
     4561 gatgctgaaa aagaagtaaa aaaacataaa cttgataaag cttatattat caaggtgaat
     4621 caaaatcgca cgttacaagg gacaatcatt agtgagaagc gagtaagtca tgaagattca
     4681 caaaaagtac aagcactgct tacagctatt caaacaaata tggtagccgg ggaattaaat
     4741 atcaacaaag aagacttaca aaaattacaa gcacaaagta aagttgataa taaggtgatt
     4801 tctaatgatg aagtcgataa agtatccgaa ggacaaaaga ttttcaacta tgctttagct
     4861 tatggcatta tctttctaat gttcttcatt gttttaaatt atgcatctca aatagctatg
     4921 gaaattgcta gtgagaaaac atcgcgtgtc attgaaatga ttattacaag tatttcacca
     4981 atccaacata tttttgctaa aattttaggc gttatagcag ttgccgtgac tcagattatt
     5041 ttaattgtat tgatggcagt tatatgcatt tttgcctttg acttaaaaga cttgctacaa
     5101 ggttttaacg tggaaatgaa tcaattgtct tggcaaatta ttattgtcgg aattataagt
     5161 agtattgttg gaattatggc gtatgtatta cttgcagcca tcctcggatc attaacatct
     5221 agaatagaag atttaaatca atctttgatg ccactgacat tacttggtat gattgcattt
     5281 tatatcgcga tattcacgat aaataatcct gatggacaac ttgctttaat cacaagttat
     5341 attccgtttt tagcgccatt tcaattagtt gtacgcgctc aaacttcagg gcttcaaata
     5401 tatgaggtca tcttaagttc tttaatctca attgtaatgg tagctgtctt gatatggatt
     5461 gcaattaaaa catataaaga ttcagtactt acatttgaac gtggcttatt taattcaatg
     5521 aaacgtgtat ttaaaaaata aaattgatga gagtgacaca catttatcat ataaaatgat
     5581 gatgaaagcg tgttactctt atttttttat atttagctat agaataatgt ctaaaaaagt
     5641 gtacaatatg aatgtgaaaa cgaacttttt aaaatgggaa tgtgaagtaa gaagaaatgt
     5701 aaacaaatga tatgtagccg atagattatt gatagcaaat gtgtgatgga gtgatttcat
     5761 gttatatccg atttttatat ttatattagc tggcttatgt gaaataggtg ggggatacct
     5821 gatttggctg tggcttaggg aaggacagtc ttcacttgtt gggctaatag gcggtgcgat
     5881 actcatgtta tatggtgtca ttgcgacatt tcaatcattt ccatcattcg gaagagtata
     5941 tgcagcatat ggtggcgtat ttatcatcat gagtttgatt tttgcgatgg tcgtagataa
     6001 acaaatgcca gataaatatg atgttatcgg tgcaatcatt tgtattgtag gtgttttagt
     6061 aatgttgcta cccagcagag cttgaacact tgtggttaga agggagaagt aaaaatgata
     6121 gttgaagttg agcaccaaag acaaggtgta gatttaatca aaattgataa tgatgaaact
     6181 aaaattattt ttacgaatta tggagcaaga gtagtgtctt ggaaatatca tgataataat
     6241 attgttcttg gaaatgttgt tgaagcggat gagttttatc atagtaatcc ttttaaattt
     6301 ggtgcttcta taggaagata tagcggtaga attgataatg ctaaatttaa gttgaaaggt
     6361 aaagaatatc agttagaaaa aaacaacggt gaacatcatt tacatggtgg atgtcatggt
     6421 ttggacaata agttatttga ttatgaaatt agaaatgaaa ttgctcaaat taaagttata
     6481 tttaagacgg tgttgaaatc agctgatgat catttcccgg gtgatatcga tgtgacgatt
     6541 acacatattt atgatgcgga tcaccaatgg tcgattgaat acgaagcagt tgcatcagaa
     6601 gacacattgt ttagtcctat gaatcatgtg tacttcaatt tgaatcgaga taataatgtc
     6661 gttgataatc atcgcatttc aagtaatcaa ttagatatgt atgtattaga tgagcgaaat
     6721 attgttacag gtgatattct agatttacat gaagtgtttg aagataacaa gataaaattg
     6781 agtgatattt ttacatcaca acatgcgcaa ttaagccaac aaatgacacg ttttggtggt
     6841 ctcgatcacc catttacagt tggtgaacat aaaatgtatg ttgaaaatca tgaatttatg
     6901 ttagaggtcg atacagatat gcctcatgtt gtgttcttta cttttaacca gcctgatgag
     6961 tgggatagtc catttaatat ttataaacca cattctggct ttacattaga aacacaattt
     7021 ttaccaaatg atattaattt atatggtgaa aaagcaccgt caattttaaa agctggtgaa
     7081 gtgttcaatt cgaaaacaag tttccgtatc gaagaaaaag tagaaaatga ttaaaagtat
     7141 cggcatttat ataatattta tgttatttgg cggaattttg aataggttat caagaatcgt
     7201 atcaa
//
LOCUS       DAHHSC010000120         7179 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.121, whole genome shotgun sequence.
ACCESSION   DAHHSC010000120 DAHHSC010000000
VERSION     DAHHSC010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 7179)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7179)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7179
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.121"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            219..1010
                     /gene="pgeF"
                     /locus_tag="NBI57_001849"
     CDS             219..1010
                     /gene="pgeF"
                     /locus_tag="NBI57_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000999349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HCD1822784.1"
                     /translation="MNDNFKKQPHHLIYEELLQQGITLGITTRGDGLSDYPKNAFNMA
                     RYIDDRPYNITQHQLQLAEEIAFDRKNWVFPIQTHENKVACITKDDIGTNIDTLTDAL
                     HGIDAMYTYDSNVLLTMCYADCVPVYFYSTKHHFIALAHAGWRGTYTEIVKEVLKHVN
                     FDLKDLHVVIGPSTSSSYEINDDIKNKFETLPIDSANYIETRGRDRHGIDLKKANAEL
                     LNYYGVPKENIYTTAYATSEHLELFFSYRLEKGQTGRMLAFIGQQ"
     gene            1034..1702
                     /locus_tag="NBI57_001850"
     CDS             1034..1702
                     /locus_tag="NBI57_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HCD1822785.1"
                     /translation="MKDNLQQISTQINDKSEKNNFSTKPNVIAVTKYVTIERAKEAYE
                     AGIRHFGENRLEGFFQKKEALPSDAVIHFIGSLQSRKVKDVINDVDYFHALDRLSLAK
                     EINKRAEHKIKCFLQVNVSGEASKHGIALEDVDQFIDDLKKYDKIEIVGLMTMAPLTD
                     DEAYIRSLFKQLRLKKEEIQRLNLEYAPCDELSMGMSNDYLIAVEEGATFVRIGTKLV
                     GEEE"
     gene            1699..2262
                     /locus_tag="NBI57_001851"
     CDS             1699..2262
                     /locus_tag="NBI57_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HCD1822786.1"
                     /translation="MSHLALKDLFSGFFVIDDEEEVEVPDKQQQVNEAPAKEQSQQTT
                     KQNAIKSVPQKSASRYTTTSEERNNRMSNYSKNNSRNVVTMNNATPNNASQESSKMCL
                     FEPRVFSDTQDIADELKNRRATLVNLQRIDKVSAKRIIDFLSGTVYAIGGDIQRVGTD
                     IFLCTPDNVEVAGSITDHIENMEHSFD"
     gene            2274..2564
                     /locus_tag="NBI57_001852"
     CDS             2274..2564
                     /locus_tag="NBI57_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HCD1822787.1"
                     /translation="MDINVLATIFKFILFVVEIYYFGMIIYFFTSWVPSIRETKVGYF
                     LAKIYEPFLQPFRKVIPPIGIIDISSIAAIIVLVLFQKGLLQIFNWILIQLQ"
     gene            2674..3453
                     /locus_tag="NBI57_001853"
     CDS             2674..3453
                     /locus_tag="NBI57_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001827493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HCD1822788.1"
                     /translation="MINIDIYQHFRQEEYELIDQLTDKCDQAEQHYAPVLTHFLDPRG
                     QYILEVICGSYEDLNVSFYGGPNAERKRAIISPNYYEPKESDFELTLMEIEYPEKFVT
                     LKHQHILGTLMSLGIEREQVGDIIVNERIQFVLTSRLESFIMLELQRIKGASVKLYTI
                     PVTDMIQSNENWKNESATVSSLRLDVVIKEMIRKSRTIAKQLIEKKRVKVNHTIVDSA
                     DFQLQANDLISIQGFGRAHITDLGGKTKKDKTHITYRTLFK"
     gene            3477..4094
                     /locus_tag="NBI57_001854"
     CDS             3477..4094
                     /locus_tag="NBI57_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HCD1822789.1"
                     /translation="MPFTPNEIKNKEFSRVKNGLEPTEVANFLEQLSTEIERLKEDKK
                     QLEKVIEERDTNIKSYQDVHQSVSDALIQAQKAGEETKQAAEKQAEAIIAKAEAQANQ
                     MVGDAVEKARRLAFQTEDMKRQSKVFRSRFRMLVEAQLDLLKNEDWDYLLNYDLDAEQ
                     VTLENIHHLHENDLKPDEVAANAQNNASNTPDNNQQSNDSETTKK"
     gene            4315..7068
                     /gene="ileS"
                     /locus_tag="NBI57_001855"
     CDS             4315..7068
                     /gene="ileS"
                     /locus_tag="NBI57_001855"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HCD1822790.1"
                     /translation="MDYKETLLMPKTDFPMRGGLPNKEPQIQEKWDAEDQYHKALEKN
                     KGNETFILHDGPPYANGNLHMGHALNKILKDFIVRYKTMQGFYAPYVPGWDTHGLPIE
                     QALTKKGVDRKKMSTAEFREKCKEFALEQIELQKKDFRRLGVRGDFNDPYITLKPEYE
                     AAQIRIFGEMADKGLIYKGKKPVYWSPSSESSLAEAEIEYHDKRSASIYVAFDVKDDK
                     GVVDADAKFIIWTTTPWTIPSNVAITVHPELKYGQYNVNGEKYIIAEALSDAVAEALD
                     WDKASIKLEKEYTGKELEYVVAQHPFLDRESLVINGDHVTTDAGTGCVHTAPGHGEDD
                     YIVGQKYELPVISPIDDKGVFTEEGGQFEGMFYDKANKAVTDLLTEKGALLKLDFITH
                     SYPHDWRTKKPVIFRATPQWFASISKVRQDILDAIENTNFKVNWGKTRIYNMVRDRGE
                     WVISRQRVWGVPLPVFYAENGEIIMTKETVNHVADLFAEHGSNIWFEREAKDLLPEGF
                     THPGSPNGTFTKETDIMDVWFDSGSSHRGVLETRPELSFPADMYLEGSDQYRGWFNSS
                     ITTSVATRGVSPYKFLLSHGFVMDGEGKKMSKSLGNVIVPDQVVKQKGADIARLWVSS
                     TDYLADVRISDEILKQTSDVYRKIRNTLRFMLGNINDFNPDTDSIPESELLEVDRYLL
                     NRLREFTASTINNYENFDYLNIYQEVQNFINVELSNFYLDYGKDILYIEQRDSHIRRS
                     MQTVLYQILVDMTKLLAPILVHTAEEVWSHTPHVKEESVHLADMPKVVEVDQALLDKW
                     RTFMNLRDDVNRALETARNEKVIGKSLEAKVTIASNDKFNASEFLTSFDALHQLFIVS
                     QVKVVDKLDDQATAYEHGDIVIEHADGEKCERCWNYSEDLGAVDELTHLCPRCQQVVK
                     SLV"
BASE COUNT         2637 a         1011 c         1359 g         2172 t
ORIGIN      
        1 agataacata tctgatgatt tttttactaa tttttagaac atgtagaagg acatttaagt
       61 ttttcgaagt cattaaaagt gtttaagtat cgtgtcaaaa ttaagtcaaa aattatttgc
      121 gcaacatttt aactttaaac ataaatgtta tattatataa ttattaactt tgtacagtta
      181 gacgaagata atttaaatga aatgatggtg acgatcgagt gaatgataat tttaaaaagc
      241 aaccgcatca tttaatatat gaagaattat tacaacaagg tattactcta ggtattacaa
      301 ctagaggaga tggtttaagc gactatccta aaaatgcttt taatatggcg agatatattg
      361 atgatcgccc atataatatt actcaacatc aattgcaatt agctgaagaa attgcgtttg
      421 atagaaaaaa ttgggtgttt cccatccaaa cacatgaaaa taaagtcgct tgtattacaa
      481 aggatgatat aggcacaaat atagacactt taactgatgc gcttcatggt attgatgcga
      541 tgtacacata tgatagtaat gtcttattaa cgatgtgtta tgcagactgt gtaccagtat
      601 atttttatag tacaaaacat cattttattg cattggcgca tgcaggttgg cgtggtacct
      661 atactgaaat tgtaaaagaa gtgctaaaac atgtgaactt tgatttgaaa gacttacatg
      721 tcgttattgg accatctaca tcatcaagtt atgaaattaa tgatgatatt aaaaataaat
      781 ttgaaacatt gccaattgat agtgccaact atattgaaac tagaggacga gatcgtcatg
      841 gtattgattt gaaaaaagcc aatgctgaat tattaaatta ttatggtgtt cctaaagaaa
      901 atatttatac gacagcgtat gctacatctg aacatttaga attatttttc tcttatcgat
      961 tagaaaaagg tcaaacagga cgcatgttag cattcattgg tcaacagtaa acaaggagga
     1021 gatatgtttg cgtgtgaaag ataatttaca acaaatctca acacaaatta atgacaaaag
     1081 tgaaaaaaat aatttttcaa caaaaccaaa cgtgattgca gttacaaaat atgttacaat
     1141 agagcgagct aaagaagcgt atgaggctgg aataagacat tttggtgaga atagattgga
     1201 aggctttttt caaaagaaag aagcattacc atcagatgcg gtgattcatt ttataggatc
     1261 attacaatct cgaaaagtta aggacgttat aaacgacgta gattatttcc atgctttaga
     1321 tcgattgagc ttagccaaag aaattaacaa acgtgcagaa cataaaatta aatgtttctt
     1381 gcaagtgaac gtttcgggag aagcttctaa acatggtatt gctttagaag atgttgatca
     1441 gtttatagat gatcttaaaa aatatgacaa aatcgaaatt gtaggtttaa tgacgatggc
     1501 accattgaca gatgatgaag catatattag atcgttattt aaacagttac gtttgaaaaa
     1561 agaagaaata caacgactca atttagaata tgcgccttgt gatgaattat caatgggaat
     1621 gagtaatgac tatcttattg cagttgaaga aggtgcgacg tttgttagaa ttgggactaa
     1681 acttgtagga gaagaggagt gagccacttg gctttaaaag atttatttag tggatttttt
     1741 gtaatagatg atgaagagga agtagaagta cctgacaaac aacaacaggt caatgaagcg
     1801 ccagcaaaag agcagtcaca acaaacaaca aaacaaaacg caatcaaatc agtccctcaa
     1861 aaatctgcat caagatatac aacaacgtca gaagaaagga ataaccgtat gtctaattat
     1921 tcaaaaaata attcacgtaa tgttgtaact atgaacaatg ctacaccaaa caatgcatca
     1981 caagaaagtt caaaaatgtg tttattcgaa ccacgtgttt tttcagatac acaagatatt
     2041 gctgatgagc ttaaaaaccg ccgtgcgaca cttgtcaatt tacaacgtat tgataaagta
     2101 tcagcgaaaa gaattattga ttttttaagc ggtactgttt atgcaatcgg tggagatatc
     2161 caacgtgtag gtactgatat tttcttatgt acgcctgata atgtggaagt agctggaagc
     2221 attacagacc atattgaaaa tatggaacat tcattcgact aaggagttaa catatggata
     2281 taaatgtgct agctacaata tttaaattta tcctttttgt tgttgaaatt tattatttcg
     2341 gcatgattat atatttcttt acatcttggg taccaagtat tagagaaact aaggtaggtt
     2401 attttttagc gaaaatatat gaacctttct tacaaccatt tagaaaagta attccaccta
     2461 ttggaattat cgacatatca tcaatcgctg caattatcgt tttagtatta ttccaaaaag
     2521 ggttactcca aatctttaat tggattttaa ttcaattaca ataaattaag cgacataacg
     2581 aggcaagggt acatgatact ttagcctcgt ttttgatatg tatttttctg aatataaggg
     2641 caatagatgg tattttataa tttttttaag gtagtgatta acatagatat ttatcaacac
     2701 tttagacagg aagaatacga attaattgat cagctaacgg ataaatgtga tcaagcggaa
     2761 caacattatg caccagtatt aacgcatttt ttagatccaa gagggcaata tatattggaa
     2821 gtgatttgtg gcagttatga agatttaaac gtctcttttt atggcgggcc taatgctgaa
     2881 agaaaaagag caatcatttc gccgaactat tatgaaccta aagaaagtga ctttgaatta
     2941 actttaatgg aaatagagta tcctgaaaaa ttcgtcactt taaaacatca acatatttta
     3001 gggacattaa tgtctttagg tatcgaacgc gaacaagttg gagatataat tgtgaatgaa
     3061 cgaattcaat ttgttttgac aagtagattg gaatcattta ttatgttaga attacaacgt
     3121 attaaaggcg catcagttaa actttatact attccagtaa cagatatgat acaatctaat
     3181 gagaattgga aaaatgaaag tgcaacagtt agttctttaa ggttagatgt tgttattaaa
     3241 gaaatgatac gtaaatcacg tacgattgcg aaacaactaa ttgaaaaaaa acgtgttaaa
     3301 gtgaatcaca ctattgttga ttcagcagat tttcaattac aagcaaatga tttaatatcc
     3361 atccaaggtt ttggtagagc acacattact gacttaggtg gtaaaactaa aaaagataaa
     3421 acgcacatta cctatagaac attattcaaa tagtaatgat ttaaggagga taacaaatgc
     3481 cttttacacc aaatgaaatt aagaataaag agttttcacg tgtaaagaat ggtttagaac
     3541 ctactgaagt tgctaatttt ttggagcaac taagcactga aattgaacgt cttaaagaag
     3601 ataaaaaaca acttgaaaaa gtaatcgaag agagagatac taatattaag tcttatcaag
     3661 acgtgcatca atctgtaagt gatgctttga tacaagctca aaaagctggt gaagaaacta
     3721 agcaagctgc agagaaacaa gctgaagcga ttatagctaa ggcagaagcg caagctaatc
     3781 aaatggttgg tgacgcggta gaaaaagcac gccgtttagc attccagact gaagatatga
     3841 aacgtcaatc aaaagtattt agatcgcgtt tccgtatgtt agttgaagcg caattagact
     3901 tattaaaaaa cgaagattgg gattacttgt tgaattatga tttagacgct gaacaagtga
     3961 cgcttgaaaa tattcatcat ttgcatgaaa atgatttaaa gccagatgaa gttgcagcaa
     4021 atgcacaaaa taatgcatca aatacaccag acaataatca acaatccaat gattcagaaa
     4081 caactaagaa gtaagaatta aataaagaca gacgcgtaat atacatttaa cttttcacag
     4141 cgaattaggt aatggtgaga gcctagtaaa agcatgtatg ttatatcact ggctttttaa
     4201 tatttaaata atgtaatgag agaactctaa gttgagttaa taagggtggt accgcgagca
     4261 atcgtccctt ttaatttaac ttagagtttt ttaaattttt aaggagtgaa aaaaatggat
     4321 tacaaagaaa cgttattaat gcctaaaaca gatttcccaa tgcgaggtgg tttaccaaac
     4381 aaggaaccgc aaattcaaga aaaatgggat gcagaagatc aataccataa agcgttagaa
     4441 aaaaataaag gtaacgaaac attcatttta catgatggcc caccatacgc gaatggtaac
     4501 ttacatatgg gacatgcctt gaacaaaatt ttaaaagact ttattgtacg ttataaaact
     4561 atgcaagggt tctatgcacc atacgtacca ggttgggata cacatggttt accaattgaa
     4621 caagcattaa cgaaaaaagg tgttgaccga aagaaaatgt caacagctga attccgtgag
     4681 aaatgtaaag aatttgcttt agaacaaatt gaattacaga aaaaagattt tagacgttta
     4741 ggtgttcgtg gtgactttaa tgatccatat attacattaa aacctgaata cgaagctgca
     4801 caaattcgta tttttggaga aatggcagat aaaggtttaa tttataaagg taaaaagcca
     4861 gtttattggt ctccttcaag tgagtcttca ttagcagaag cagaaattga atatcacgat
     4921 aaacgttcag catcaattta cgttgcattt gacgttaaag atgacaaagg tgtcgttgat
     4981 gcagatgcta aatttattat ctggacaaca acgccatgga caattccatc aaatgttgcg
     5041 attaccgttc atcctgaatt aaaatatggt caatacaatg taaatggcga aaaatatatt
     5101 attgcagaag ccttgtctga cgctgtagca gaagcactgg attgggataa agcatcaatc
     5161 aaattagaaa aagaatacac aggtaaagaa ttagagtatg ttgtagcaca acatccattc
     5221 ttagacagag aatcgttagt gattaatggt gatcatgtta ctacagatgc tggtacaggt
     5281 tgtgtacata cagcaccagg tcacggggaa gatgactata ttgttggtca aaaatatgaa
     5341 ttgccagtaa ttagtccaat cgatgataaa ggtgtattta ctgaagaagg cggccaattt
     5401 gaagggatgt tctatgataa agctaataaa gccgttactg atttattaac agaaaaaggt
     5461 gcactattaa aattagactt tattacacat agctatccac acgactggag aacaaaaaaa
     5521 cctgtaatct tccgtgctac accacaatgg tttgcctcaa tcagtaaagt aagacaagat
     5581 attttagatg caatcgaaaa tacaaacttc aaagtaaatt ggggtaaaac acgtatttac
     5641 aatatggttc gtgaccgtgg cgaatgggtt atttctcgtc aacgtgtgtg gggtgtaccg
     5701 ttaccagtat tttatgctga aaatggcgaa attatcatga cgaaagaaac agtgaatcat
     5761 gttgctgatt tatttgcaga acacggttca aatatttggt ttgaaagaga agcgaaagac
     5821 ttactaccag aaggatttac acatccaggc agccctaacg gtacatttac taaagaaaca
     5881 gacattatgg acgtttggtt tgattctggt tcatcacacc gtggcgtgtt ggaaacaaga
     5941 ccggaattaa gtttcccagc ggatatgtat ttagaaggta gtgaccaata tcgtggttgg
     6001 ttcaactctt ctatcacaac ttcagttgct acaagaggag tatcacctta taaattctta
     6061 ctttctcatg gttttgttat ggacggtgaa ggtaagaaaa tgagtaaatc tttaggtaat
     6121 gtgattgtac ctgaccaagt ggttaaacaa aaaggtgctg atattgcgag actttgggta
     6181 agtagtacgg actatttagc tgatgttaga atttctgatg aaattttaaa acaaacatct
     6241 gatgtttatc gtaaaatcag aaatacatta agatttatgt taggtaacat taacgatttc
     6301 aatcctgaca cagatagcat tcctgaatca gagttattag aagtggatcg ttacttgcta
     6361 aatcgtttac gtgaatttac tgcaagtacg attaacaact atgaaaactt tgactactta
     6421 aatatttatc aagaagttca aaactttatc aatgttgagt taagtaattt ctatttggat
     6481 tacggtaaag atattttata tattgaacaa cgtgattctc atatccgtcg tagtatgcaa
     6541 acagtgttat atcaaatttt agttgatatg acgaagttgt tagcaccaat cttagtgcat
     6601 acagctgaag aagtttggtc tcatacacca catgttaaag aagaaagtgt tcacttagca
     6661 gacatgccta aagttgtaga agtagatcaa gctttattgg ataaatggcg tacatttatg
     6721 aatttacgtg atgatgtgaa ccgtgcatta gaaactgctc gtaatgaaaa agttattggt
     6781 aaatcattag aagctaaagt tacgattgct agtaacgata aatttaatgc atctgaattc
     6841 ttaacttcat ttgatgcatt acatcaatta tttatcgtgt cacaagttaa agttgtagat
     6901 aagttagatg atcaggcaac agcttatgaa catggtgata ttgtcatcga acatgcagat
     6961 ggtgaaaaat gtgaaagatg ttggaactat tcagaggatc ttggtgctgt tgatgaattg
     7021 acgcatctat gtccacgatg ccaacaagtt gtaaaatcac tggtataatt gaaattgtat
     7081 aaagtactta tacagatgat ataaattaaa gctctcttca taatcatgtt gtagtttttg
     7141 ttgacatgat gaagagagtt tttttgtgaa taaaaaaat
//
LOCUS       DAHHSC010000121         7122 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.122, whole genome shotgun sequence.
ACCESSION   DAHHSC010000121 DAHHSC010000000
VERSION     DAHHSC010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 7122)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7122)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7122
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.122"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            complement(87..1448)
                     /locus_tag="NBI57_001856"
     CDS             complement(87..1448)
                     /locus_tag="NBI57_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCD1822791.1"
                     /translation="MAEKLQRELSNRHIQLIAIGGAIGTGLFLGAGQTIALTGPSILL
                     TYIIIGFMLFMFMRGLGEIIIQNTEFKSFADVTNTYIGPFAGFVTGWTYWFCWIITGM
                     AEVTAVAKYVSFWFPEIPNWISALFCVLLLMSFNLLSARLFGELEFWFSIIKIATIIG
                     LIVVGFVMILFAFKTQFGHASFTNLYEHGIFAKGASGFFMSFQMALFSFVGIEMIGVT
                     AGETKDPVKTIPKAINSVPIRILIFYVGALAVIMSIIPWQQVDPDNSPFVKLFALIGI
                     PFAAGLINFVVLTAAASSCNSGIFSNSRMLFGLSSQQQAPPNFSKTNKYGVPHVAIFA
                     SSALLLVAALLNYIFPDATKVFTYVTTISTVLFLVVWGLIIIAYINYSRKNPDLHKNA
                     TYKLLGGKYMGYLIFVFFIFVFGLLFINVDTRRAIYFIPIWFILLAFMYLRYKRIAAK
                     SNK"
     gene            1796..2917
                     /locus_tag="NBI57_001857"
     CDS             1796..2917
                     /locus_tag="NBI57_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001662816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HCD1822792.1"
                     /translation="MAELQRGLEGVIAAETKISSIIESQLTYAGYDIDDLAENAQFEE
                     VIFLLWNYRLPNEEELAHLKGKLNQYMTLNPRVYTHFEEYVTDHVHPMTALRTSLSYI
                     AHFDPDAENESDENRYERAMRIQAKVASLVTAFARVRQDKEPLKPNPDLSYAANFLYM
                     LRGELPTDIEVEAFNKALILHADHELNASAFTARCAVSSLSDMYSGIVAAVGSLKGPL
                     HGGANEQVMTMLSEIGSIENVDAYLDEKFANKDKVMGFGHRVYKDGDPRAKYLREMSR
                     QITKDAGREELFEMSVKMEKRMAEEKGLIPNVDFYSASVYHCMEIPHDLFTPIFAVSR
                     SAGWIAHILEQYKDNRIMRPRAKYIGETNRKYIPLEERK"
     gene            2966..4234
                     /gene="icd"
                     /locus_tag="NBI57_001858"
     CDS             2966..4234
                     /gene="icd"
                     /locus_tag="NBI57_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HCD1822793.1"
                     /translation="MTAEKITQGTEGLNVPNEPIIPFIIGDGIGPDIWKAASRVIDAA
                     VEKAYNGEKRIEWKEVLAGQKAFDTTGEWLPQETLDTIKEYLIAVKGPLTTPIGGGIR
                     SLNVALRQELDLFTCLRPVRWFKGVPSPVKRPQDVDMVIFRENTEDIYAGIEFKEGTT
                     EVKKVIDFLQNEMGATNIRFPETSGIGIKPVSKEGTERLVRAAIQYAIDNNRKSVTLV
                     HKGNIMKFTEGSFKQWGYDLALSEFGDQVFTWQQYDEIVENEGRDAANAAQEKAEKEG
                     KIIIKDSIADIFLQQILTRPAEHDVVATMNLNGDYISDALAAQVGGIGIAPGANINYE
                     TGHAIFEATHGTAPKYAGLNKVNPSSVILSSVLMLEHLGWQEAADKITDSIEDTIASK
                     VVTYDFARLMDGAEEVSTSAFADELIKNLK"
     gene            4624..5328
                     /locus_tag="NBI57_001859"
     CDS             4624..5328
                     /locus_tag="NBI57_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCD1822794.1"
                     /translation="MSQKVLVVDDEHSIVTLLKYNLETAGYEVVVAFDGDEALEKVES
                     EQPDLIILDVMLPKKDGIDVCKTVRTNKNLVPILMLTAKNDEFDRVLGLELGADDYMT
                     KPFSPREVVARVKAILRRSQFVKEVEEIDDDITIGSIRIRPDYFEVYKHNELLELTPK
                     EFELLLYLIERQGRVITREHMLNTVWNYEFAGDSRIVDVHISHLRDKLEDNPKKPQLI
                     KTVRGLGYKLERPKEQ"
     gene            5328..6989
                     /locus_tag="NBI57_001860"
     CDS             5328..6989
                     /locus_tag="NBI57_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HCD1822795.1"
                     /translation="MKFHHRLMLLISSILIISFVTLGIIIHASITYVLSEKHEQALAT
                     DARSYIYLVQDNEIDKVKEIVKQQNIDLLITKHDKKVFSSGKLKAIPNSDSYIQKRDV
                     FIFNKKVNGYHVWIKGYNNDITEMHWTLWKYLILTCLVVLICLYFASRSFKRTLIRPI
                     QEVTYATQLLANGYYHIRVPESNVVETKALFVSTNDLARRLQKLNNEQKIQSNRLKTT
                     IENIPSAILMIDRNGKIVVANKAYYEQFNISHNIEQVGYHGYVNTEIEQLILESFKVE
                     KPIYEQLEVAINQVHAKYFDISCVPILTRSQKSLQGILVVMHDITNLKQLENLRREFV
                     ANVSHELKTPITSIKGFAETLIDGAKNDAESLDMFLNIILKESNRIESLVTDLLDLSH
                     IEQHTELDTDYMNLSDLTRRIIDNMMTQANQKNISIHTDIEKDVIVKAQESKIAQVIT
                     NLLTNAINYSYEDGDINVRVYRDDFRVIFEVQDFGIGIKLEDQQRIFERFYRVDKARS
                     RDSGGTGLGLSITKHIVEAHQGNIEVNSQVGKGSTFKVILKDYKE"
BASE COUNT         2571 a         1022 c         1329 g         2200 t
ORIGIN      
        1 gagccaagta cgcaatttaa tattataagt tgcatataaa aacccctgtt gaactttagt
       61 atattcaaca ggggtaaaac ttgttgttat ttatttgatt tagcagcgat acgtttatat
      121 cttaagtaca taaatgctaa aagtataaac caaatcggaa taaaataaat tgcacgtctt
      181 gtatcaacat taataaataa taacccgaac acaaaaatga agaatacaaa tattaagtag
      241 cccatatatt taccacctaa tagtttgtac gtagcatttt tatgtagatc tgggttttta
      301 cgactataat tgatatatgc aatgataatc agaccccata caactaaaaa taacactgta
      361 gagatggtag tcacatacgt aaatactttt gtcgcatctg ggaaaatata gtttagtaat
      421 gctgccacaa gtaataatgc tgatgaagca aagattgcaa catgtggaac gccatattta
      481 ttcgtcttag aaaagttcgg aggtgcttgt tgttgacttg ataaaccgaa aagcatacgg
      541 ctatttgaga atataccact gttacatgat gaagcagcag cggttaatac tacaaaatta
      601 atcaagcccg cagcaaacgg aattccgatc aatgcgaata attttacgaa tggactgtta
      661 tcaggatcaa cttgctgcca agggataata gacatgataa ccgctaacgc accaacgtaa
      721 aatattaaaa ttctaatggg tacactgtta attgcctttg gaattgtttt aactggatct
      781 tttgtttccc cagctgtaac accaatcatt tcaattccta caaatgagaa taatgccatt
      841 tggaaagaca taaagaatcc agaagcacct ttagcgaata tgccgtgttc atataaattt
      901 gtgaaactgg catgcccgaa ttgagtttta aatgcaaata gaatcatgac gaaaccaact
      961 actattaaac caataattgt cgctatttta atgatagaga accaaaattc taattctccg
     1021 aaaagtcttg cgctaagtag gttgaatgac attaataaca gtacacaaaa tagtgcactt
     1081 atccagtttg gaatttctgg gaaccaaaag ctaacatatt ttgccacagc cgttacttca
     1141 gccatacctg taataatcca acagaaccag tatgtccatc cggtaacaaa tcctgcaaaa
     1201 ggcccaatat atgtattggt tacatctgcg aaagatttaa attcagtatt ctgtataatg
     1261 atttctccta aacctcgcat aaacataaat aacataaatc ctataatgat gtatgttaat
     1321 agaattgaag ggccggttaa tgcaatcgtt tgaccagcac ctaagaataa gcctgtacca
     1381 attgcaccgc caattgcaat taattgtatg tggcgattgc tcagttccct ttgtaatttt
     1441 tcagccataa tacatctccc ttaaatatag atatgtttat tatgcactta tattgagata
     1501 tatacaatta ttttcggtaa aaatgtgtaa aattccatgt taatatactt tggtttttat
     1561 aatcatatat aataaccaat tgaaaattta attctattgt aaaattcatg gattattcac
     1621 atcttgaaaa agctttaatg gtgctatttg tggctattct gtgacattta catagattta
     1681 caaaaaaatt gttgcacata taatgccagt ttttatattt cacaaacgaa atgcgtttag
     1741 ctataatatt agttgaaagc catttcataa agaaacagta aaggggaaat ttatcatggc
     1801 agaattacaa agaggtttag aaggggttat cgcagcggag actaaaataa gttcaattat
     1861 tgaaagtcaa ttgacttatg ccggctatga tattgatgat ctagctgaaa atgcgcaatt
     1921 tgaagaagtt attttcctat tatggaacta tagattgcca aacgaagaag agcttgctca
     1981 tctaaaaggg aaattaaatc aatacatgac attaaatcct cgtgtgtata cacattttga
     2041 ggagtatgtt acagatcacg tgcatccaat gacagcatta cgtacgtcat tatcatatat
     2101 tgcacatttc gatcctgatg ctgaaaatga atcagatgaa aatcgttatg aaagagcaat
     2161 gcgtatacag gctaaagtag catcattagt tacagcgttt gctcgagtaa gacaagataa
     2221 agaaccactt aagcctaatc ctgacttaag ttatgcggca aacttcctat atatgttacg
     2281 tggggaatta ccaacagata tagaagtaga agccttcaat aaagcactta ttttacacgc
     2341 tgatcatgag ttgaacgcat ctgcatttac agcacgttgt gcggtatcat cattgtcaga
     2401 tatgtactca ggtattgtag cagctgtagg ttctctgaaa gggccattac atggtggtgc
     2461 aaacgaacaa gttatgacga tgttatctga gattgggtca attgaaaatg ttgatgctta
     2521 cttagatgaa aaatttgcta ataaagataa agtaatgggc tttggtcatc gtgtatataa
     2581 agatggtgat cctagagcga aatatttaag agaaatgagc cgtcaaatta cgaaagacgc
     2641 tggtcgtgaa gaattatttg aaatgtcagt gaaaatggaa aaacgtatgg cagaagaaaa
     2701 aggattaatt cctaatgttg atttttatag tgcgagtgtt tatcactgta tggaaatacc
     2761 tcatgactta ttcacgccaa tctttgctgt aagtcgttct gcaggatgga ttgctcatat
     2821 tttagaacaa tataaagata atagaattat gcgtcctaga gcgaaatata ttggcgaaac
     2881 gaatcgtaag tatatcccgc ttgaagaaag aaaataatca atacaaatta aaaatgaaga
     2941 tgtaaaattt ggaggtaaaa taactatgac tgcagaaaaa attactcaag gaactgaagg
     3001 attaaacgta cctaatgaac caattatccc atttattatc ggtgatggaa ttggaccgga
     3061 tatttggaag gcagcaagcc gagttataga tgctgctgtt gagaaagcct ataatggcga
     3121 aaaacgcatt gaatggaaag aagtgctagc tggccaaaaa gcatttgata caactggtga
     3181 atggttacct caagaaacac ttgatacaat taaagaatat ttaattgctg ttaaaggacc
     3241 tttaacaaca ccaattggtg gtggtattag atcattaaat gtggctttac gccaagaatt
     3301 agatttattt acttgcttaa gaccggtacg ttggtttaaa ggagtaccat cacctgttaa
     3361 acgtccacaa gatgttgata tggttatttt ccgtgaaaat actgaagaca tttatgctgg
     3421 tattgaattt aaagaaggta caacagaagt taaaaaggta attgacttct tacaaaacga
     3481 aatgggtgcg acaaacattc gattcccaga aacttcaggt attggtatta aaccagtttc
     3541 taaagaagga actgagcgat tagttagagc agctatacaa tatgctatcg ataataaccg
     3601 taaatcagtt actttagttc ataaaggtaa tattatgaaa tttacagaag gctcatttaa
     3661 gcagtggggt tacgatttag cattatctga atttggtgat caagtattca cttggcaaca
     3721 atatgacgaa attgttgaaa atgaaggcag agatgctgct aatgctgctc aagaaaaagc
     3781 tgaaaaagaa ggcaagatta tcattaaaga ttctattgct gacattttct tacaacaaat
     3841 tttaactcgt ccagctgagc atgatgttgt agcaactatg aacttgaatg gtgactatat
     3901 ttcagatgct ttagctgcac aagttggtgg tattggtatt gcgccaggtg caaacattaa
     3961 ttatgaaaca ggtcatgcta tttttgaagc aacacatggt acagctccaa aatatgcagg
     4021 tttaaataaa gtgaatccat cttcagtaat tttaagttct gtattaatgt tagaacattt
     4081 aggatggcaa gaagcggcag ataagattac agattcaatt gaagatacaa ttgcttcaaa
     4141 agttgttact tatgactttg cccgtttaat ggatggtgct gaagaagttt ctacatcagc
     4201 atttgcagat gaattgatta aaaatttaaa ataagcaaaa taaaattagg tgagtctagc
     4261 aactttaatt gctaggttcg cctttttaaa tgaattgaag ttggcaacag gatagaaata
     4321 ataaagagtc accaatgatt gattatgtag tgtttattac acattagaca aagcgaattt
     4381 gtacataagc ttaggaataa atgagtaaaa ctcatgcata ggtagtggga tagaaatgat
     4441 atttttacaa aatttatttc gttgtcccag agtaagtcat gcaaatgata gaaggaaata
     4501 taagaagtat tttatttata tgtaaagtaa tgtaaaaaat gtgtaaagat aatgtgtagg
     4561 atgttatttt tataaatagt tcggttataa tgtaaatata attataagtt agggaggcat
     4621 accatgtcgc aaaaagtgtt ggtagtagat gacgaacatt caatcgtaac attacttaaa
     4681 tataacttag aaacagctgg ttatgaagtt gttgtcgcat ttgatggtga tgaggcttta
     4741 gaaaaggtag aaagtgaaca gccagattta attattttag atgttatgct acctaaaaaa
     4801 gatggcattg acgtatgtaa gactgtaaga acaaataaaa atttagtccc tatattaatg
     4861 ttaacggcga aaaatgatga gtttgatcgg gtattaggtt tagaattagg tgccgatgac
     4921 tatatgacta agcctttttc acctagagaa gttgttgccc gtgtgaaagc cattttaaga
     4981 cgttctcaat ttgtaaaaga agtagaagag attgacgatg atattaccat cggatcgata
     5041 cgtattagac ccgattattt tgaagtatat aagcataatg agttattgga gctaacaccg
     5101 aaagaatttg aactcttgct ttatttgata gaaagacaag gcagagtcat tacgagagag
     5161 catatgttaa atacggtatg gaattatgaa tttgcaggag actcaagaat tgttgatgta
     5221 catatcagtc atttaagaga taaattagaa gataatccta agaagccaca acttattaaa
     5281 actgtgcgtg gcttaggcta taaattggaa agacctaaag aacaatgatg aagtttcacc
     5341 accgcttaat gttattgata agcagtatat taattatcag ttttgttaca ttaggcatta
     5401 ttattcacgc ttccatcaca tatgttttat cagaaaaaca cgaacaagct ctagcaacag
     5461 atgcaagatc atatatttat ttagtccaag ataatgagat tgataaagtt aaagaaattg
     5521 taaaacagca aaatatagat ttattgataa ctaaacatga taaaaaggtg tttagtagtg
     5581 gcaaactgaa agctatccca aatagtgata gctatattca aaaaagggac gtttttattt
     5641 ttaataagaa ggttaacggt tatcatgtat ggattaaagg ttacaataac gatattacag
     5701 aaatgcattg gacgttgtgg aaatatctta tattgacatg tttagttgta ctcatttgtc
     5761 tgtattttgc aagccgtagt tttaagcgta cattaatccg tcccattcaa gaagttactt
     5821 atgcaacaca attgttggca aatggttatt atcacatacg tgtacctgaa agtaatgttg
     5881 tagaaacgaa agcgcttttt gtgtctacca atgatttagc acgacgcctt caaaagctta
     5941 ataatgagca aaaaattcaa tccaaccgtt taaaaacaac gattgaaaat attccaagtg
     6001 ctattttaat gatagatcga aatggaaaaa tcgttgttgc aaataaagcg tattatgaac
     6061 agttcaacat ctcgcataat attgaacaag taggctatca cggctatgtt aatactgaaa
     6121 ttgagcaatt aattttagaa agttttaaag ttgaaaaacc tatttatgaa caattggaag
     6181 ttgcaataaa ccaagtccat gccaagtact ttgatatttc atgcgttccg attttaacta
     6241 ggtctcaaaa aagtttgcaa gggattttag ttgtgatgca tgatataaca aatctgaaac
     6301 aacttgaaaa tctaagacgt gaatttgtag caaatgtttc acatgaatta aaaacaccta
     6361 ttacttcaat taaggggttt gctgaaacgc ttattgatgg tgcaaaaaat gatgcggaat
     6421 cattagacat gtttttaaac ataattttaa aagaatcaaa tcgaatagaa tctttagtaa
     6481 cagatctgtt agatttatcg catattgagc aacatacaga gttagataca gactatatga
     6541 atttatcaga tttaacgcgg cgtattattg ataatatgat gactcaagcc aatcaaaaaa
     6601 atatttccat tcatactgat attgaaaaag atgtcattgt taaagcacaa gaaagtaaaa
     6661 ttgctcaagt tatcacgaat ttgttaacga atgcaattaa ctattcttat gaagatggag
     6721 atattaacgt tcgtgtgtat cgagatgact ttcgtgtcat tttcgaagta caagattttg
     6781 gtataggtat taaattagaa gaccaacaac gtatttttga aagattttat agagtagata
     6841 aagcacgaag tagagattct ggtgggacag gtcttggatt atcaattaca aagcatatag
     6901 tagaggccca ccaaggcaat attgaagtga atagtcaagt tggcaaaggc tcaacgttca
     6961 aagttattct aaaagattat aaagaataaa aattaaagtg gtaacagcgc gtgtattttt
     7021 cacgaagttg ttgctgcttt tttatttcgt tcaaatctct attggtatta aattagagtt
     7081 aatgaattgg aataaattcg gttgtcaatt ttgtcattag ca
//
LOCUS       DAHHSC010000122         7069 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.123, whole genome shotgun sequence.
ACCESSION   DAHHSC010000122 DAHHSC010000000
VERSION     DAHHSC010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 7069)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7069)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7069
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.123"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            133..6912
                     /gene="sasA"
                     /locus_tag="NBI57_001861"
     CDS             133..6912
                     /gene="sasA"
                     /locus_tag="NBI57_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-rich repeat glycoprotein adhesin SasA"
                     /protein_id="HCD1822796.1"
                     /translation="MSKRQKAFHDSLANEKTRVRLYKSGKNWVKSGIKEIEMFKIMGL
                     PFISHSLVSQDNQSISKKMTGYGLKTTAVIGGAFTVNMLHDQQAFAASDAPLTSELNT
                     QSETVGNQNSTTIEASTSTADSTSVTKNSSSVQTSNSDTVSSEKSEKVTSTTNSTSNQ
                     QEKLTSTSESTSSKNTTSSSDTKSVASTSSTEQPINTSTNQSTASNNTSQSTTPSSVN
                     LNKTSTTSTSTAPVKLRTFSRLAMSTFASAATTTAVTANTITVNKDNLKQYMTTSGNA
                     TYDQSTGIVTLTQDAYSQKGAITLGTRIDSNKSFHFSGKVNLGNKYEGHGNGGDGIGF
                     AFSPGVLGETGLNGAAVGIGGLSNAFGFKLDTYHNTSKPNSAAKANADPSNVAGGGAF
                     GAFVTTDSYGVATTYTSSSTADNAAKLNVQPTNNTFQDFDINYNGDTKVMTVKYAGQT
                     WTRNISDWIAKSGTTNFSLSMTASTGGATNLQQVQFGTFEYTESAVTQVRYVDVTTGK
                     DIIPPKTYSGNVDQVVTIDNQQSALTAKGYNYTSVDSSYASTYNDTNKTVKMTNAGQS
                     VTYYFTDVKAPTVTVGNQTIEVGKTMNPVVLTTTDNGTGTVTNTVTGLPSGLSYDSAT
                     NSIIGTPTKIGQSTVTVVSTDQANNKSTTTFTINVVDTTAPTVTPIGDQSSEVYSPIS
                     PIKIATQDNSGNAVTNTVTGLPSGLTFDSTNNTISGTPTNIGTSTISIVSTDASGNKT
                     TTTFKYEVTRNSMSDSVSTSGSTQQSQSVSTSKADSQSASTSTSGSIVVSTSASTSKS
                     TSVSLSDSVSASKSLSTSESNSVSSSTSTSLVNSQSVSSSMSGSVSKSTSLSDSISNS
                     NSTEKSESLSTSTSDSLRTSTSLSDSLSMSTSGSLSKSQSLSTSISGSSSTSASLSDS
                     TSNAISTSTSLSESASTSDSISISNSIANSQSASTSKSDSQSTSISLSTSDSKSMSTS
                     ESLSDSTSTSGSVSGSLSIAASQSVSTSTSDSMSTSEIVSDSISTSGSLSASDSKSMS
                     VSSSMSTSQSGSTSESLSDSQSTSDSDSKSLSLSTSQSGSTSTSTSTSASVRTSESQS
                     TSGSMSASQSDSMSISTSFSDSTSDSKSASTASSESISQSASTSTSGSVSTSTSLSTS
                     NSERTSTSVSDSTSLSTSESDSISESTSTSDSISEAISASESTSISLSESNSTSDSES
                     QSASAFLSESLSESTSESTSESVSSSTSESTSLSDSTSESGSTSTSLSNSTSGSASIS
                     TSTSISESTSTFKSESVSTSLSMSTSTSLSNSTSLSTSLSDSTSDSKSDSLSTSMSTS
                     DSISTSKSDSISTSTSLSGSTSESESDSTSSSESKSDSTSMSISMSQSTSGSTSTSTS
                     TSLSDSTSTSLSLSASMNQSGVDSNSASQSASNSTSTSTSESDSQSTSTYTSQSTSQS
                     ESTSTSTSLSDSTSISKSTSQSGSTSTSASLSGSESESDSQSISTSASESTSESASTS
                     LSDSTSTSNSGSASTSTSLSNSASASESDSSSTSLSDSTSASMQSSESDSQSTSASLS
                     DSLSTSTSNRMSTIASLSTSVSTSESGSTSESTSESDSTSTSLSDSQSTSRSTSASGS
                     ASTSTSTSDSRSTSASTSTSMRTSTSDSQSMSLSTSTSTSMSDSTSLSDSVSDSTSDS
                     TSASTSGSMSVSISLSDSTSTSTSASEVMSASISDSQSMSESVNDSESVSESNSESDS
                     KSMSGSTSVSDSGSLSVSTSLRKSESVSESSSLSGSQSMSDSVSTSDSSSLSVSTSLR
                     SSESVSESDSLSDSKSTSGSTSTSTSGSLSTSTSLSGSESVSESTSLSDSISMSDSTS
                     TSDSDSLSGSISLSGSTSLSTSDSLSDSKSLSSSQSMSGSESTSTSVSDSQSSSTSNS
                     QFDSMSISASESDSMSTSDSSNISGSNSTSTSLSTSDSMSGSVSVSTSTSLSDSISGS
                     TSVSDSSSTSTSTSLSDSMSQSQSTSTSASGSLSTSISTSMSMSASTSSSQSTSVSTS
                     LSTSDSISDSTSISISGSQSTVESESLSTSDSDSTSTSTSDSTSGSTSTSISESLSTS
                     GSGSTSVSDSTSMSESDSTSVSMSQDKSDSTSISDSESVSTSTSTSLSTSDSTSTSES
                     LSTSMSGSQSISDSTSTSMSGSTSTSESNSMHPSDSMSMHHTHSTSTSRLSSEATTST
                     SESQSTLSATSEVTKHNGTPAQSEKRLPDTGDSIKQNGLLGGVMTLLVGLGLMKRKKK
                     KDENDQDDSQA"
BASE COUNT         2572 a         1380 c         1303 g         1814 t
ORIGIN      
        1 agacgaataa aaatgaattt ttacaaattt taacaaaatg agtatttttg tatcaaaaaa
       61 gagttgtatt aaattgaata gtggcatata ttataatcta atgaatgtaa agataataca
      121 aggagttatt acatgagtaa aagacagaaa gcatttcatg acagcttagc aaacgaaaaa
      181 acaagagtaa gactttataa atctggaaaa aattgggtaa aatccggaat taaagaaata
      241 gaaatgttca aaattatggg gctaccattt attagtcata gtttagtgag tcaagataat
      301 caaagcatta gtaaaaaaat gacgggatac ggactgaaaa ctacggcggt tattggtggt
      361 gcattcacgg taaatatgtt gcatgaccag caagcttttg cggcttctga tgcaccatta
      421 acttctgaat taaacacaca aagtgaaaca gtaggtaatc aaaactcaac gacaatcgaa
      481 gcatcaacat caacagccga ttccacaagt gtaacgaaaa atagtagttc ggtacaaaca
      541 tcaaatagtg acacagtctc aagtgaaaag tctgaaaagg tcacttcgac aactaatagt
      601 acaagcaatc aacaagagaa attgacatct acatcagaat caacatcctc aaagaatact
      661 acatcaagtt ctgatactaa atctgtagct tcaacttcaa gtacagaaca accaattaat
      721 acatcaacaa atcaaagtac tgcatcaaat aacacttcac aaagcacaac gccatcttcg
      781 gtcaacttaa acaaaactag cacaacgtca actagcaccg caccagtaaa acttcgaact
      841 ttcagtcgct tagctatgtc aacatttgcg tcagcagcga cgacaaccgc agtaactgct
      901 aatacaatta cagttaataa agataactta aaacaatata tgacaacgtc aggtaatgct
      961 acctatgatc aaagtaccgg tattgtgacg ttaacacagg atgcatacag ccaaaaaggt
     1021 gctattacat taggaacacg tattgactct aataagagtt ttcatttttc tggaaaagta
     1081 aatttaggta acaaatatga agggcatgga aatggtggag atggtatcgg ttttgccttt
     1141 tcaccaggtg tattaggtga aacagggtta aacggtgccg cagtaggtat tggtggctta
     1201 agtaacgcat ttggcttcaa attggatacg tatcacaata catctaaacc aaattcagct
     1261 gcaaaggcga atgctgaccc atctaatgta gctggtggag gtgcgtttgg tgcatttgta
     1321 acaacagata gttatggtgt tgcaacaacg tatacatcaa gttcaacagc tgataatgct
     1381 gcgaagttaa atgttcaacc tacaaataac acgttccaag attttgatat taactataat
     1441 ggtgatacaa aggttatgac tgtcaaatat gcaggtcaaa catggacacg taatatttca
     1501 gattggattg cgaaaagtgg tacgaccaac ttttcattat caatgacagc ctcaacaggt
     1561 ggcgcgacaa atttacaaca agtacaattt ggaacattcg aatatacaga gtctgctgtt
     1621 acacaagtga gatacgttga tgtaacaaca ggtaaagata ttattccacc aaaaacatat
     1681 tcaggaaatg ttgatcaagt cgtgacaatc gataatcagc aatctgcatt gactgctaaa
     1741 ggatataact acacgtccgt cgatagttca tatgcgtcaa cttataatga tacaaataaa
     1801 actgtaaaaa tgacgaatgc tggacaatca gtgacatatt attttactga tgtaaaagca
     1861 ccaactgtaa ctgtaggcaa tcaaaccata gaagtgggta aaacaatgaa tcctgttgtg
     1921 ttgactacaa cggataatgg tactgggact gtgacaaata cagttacagg attaccaagc
     1981 ggattaagtt acgatagtgc aacgaattca atcattggga caccaacaaa aattggtcaa
     2041 tcaacagtga cagttgtgtc tactgaccaa gcaaataaca aatcgacgac aacttttaca
     2101 ataaatgttg tggatacgac agcaccaaca gtgacaccaa taggagatca atcatcagaa
     2161 gtgtattcac caatatcccc gattaaaatt gctacgcaag ataacagtgg aaatgcggtg
     2221 acgaatacag tgactggatt gccatccgga ctaacatttg atagtacaaa taatactatt
     2281 agtggtacac caacaaacat tggtacaagt actatatcaa tcgtttctac agatgcgagc
     2341 ggtaacaaaa cgacgacaac ttttaaatat gaagtaacaa gaaatagcat gagtgattcc
     2401 gtatcaacat caggaagtac acaacaatct caaagtgtct caacaagtaa agctgactca
     2461 caaagtgcat caacgagtac atcaggatcg attgtggtat ctacatcagc tagtacctcg
     2521 aaatcgacaa gtgtaagcct atctgattct gtgagtgcat ctaagtcatt aagcacatct
     2581 gaaagtaata gtgtatcaag ctcaacaagc acaagtttag tgaattcaca aagtgtatca
     2641 tcaagcatgt caggttcagt tagtaaatca acatcattaa gcgattcgat ttcgaactct
     2701 aacagtactg aaaaatccga aagtctatca acaagtacat ctgattcatt gcgtacatca
     2761 acatcactca gtgactcatt aagtatgagt acatcaggaa gcttgtctaa gtcacaaagc
     2821 ttatcaacga gtatatcagg gtcgtctagt acatcagcat cattaagtga cagtacatcg
     2881 aatgcaatta gtacatcaac atcattgagc gagtcagcta gcacctcgga ctctatcagt
     2941 atttcaaata gcatagccaa ctctcaaagt gcgtcaacaa gcaaatcaga ttcacaaagt
     3001 acatcaatat cattaagtac aagtgattca aaatcgatga gtacatcaga atcattgagc
     3061 gattcgacga gcacaagtgg ttctgtttct ggatcactaa gcatagcagc atcacaaagt
     3121 gtctcaacaa gtacatcaga ctcgatgagt acttcagaga tagtaagtga ctctatcagt
     3181 acaagtgggt cattatctgc atcagacagt aaatcaatgt ctgtaagtag ttcaatgagc
     3241 acgtctcagt caggtagtac atcagaatca ttaagtgatt cacaaagtac atctgattct
     3301 gatagtaagt cattatcact aagtactagt caatcaggtt caacaagtac atcaacgtcg
     3361 acaagtgctt cagtacgtac ttcggaatcg caaagtacgt ctggttcaat gagtgcaagt
     3421 caatccgatt caatgagcat atcaacgtcg tttagtgatt caacgagtga tagcaaatca
     3481 gcatcaactg catcaagtga atcaatatca caaagtgctt ctacgagcac atctggttcg
     3541 gtaagtactt cgacatcgtt aagtacaagt aattcagaac gtacatcaac atctgtgagt
     3601 gattccacaa gcttaagtac atcagagtct gattcaataa gtgaatcaac gtcaacgagc
     3661 gattctataa gtgaagcaat atctgcttca gagagcacgt ctatatcatt aagtgaatca
     3721 aatagtacta gcgattcaga atcacaaagt gcatctgcct ttttaagtga atcattaagt
     3781 gaaagtacgt ctgaatcaac atcagagtca gtgagtagtt cgacaagtga gagtacgtca
     3841 ttatcagaca gtacatcaga atctggtagc acatcaacat cattaagtaa ttcaacaagt
     3901 ggtagtgcgt ccatttcaac atcgacaagt atcagtgaat caacgtcaac gtttaagagc
     3961 gagagtgttt caacatcact gagtatgtca acgagtacaa gtttgtctaa ctctacaagt
     4021 ttgtcaacat cattaagtga ttccacaagt gatagtaagt ctgattcatt aagtacatca
     4081 atgtcgacaa gtgattcaat cagtacaagt aaatctgatt ccattagtac atctacgtca
     4141 ttaagtggtt ctacaagtga aagtgaatcc gactcaacat catcaagtga aagtaaatcc
     4201 gattcaacat caatgagcat aagtatgtct caatcaacat caggaagtac aagtacgtca
     4261 acgagtacaa gtttgtctga ctcaacgagt acgtcattgt cactaagtgc atcaatgaat
     4321 caaagcggag tagactcaaa ctcagcaagc caaagtgcct caaactcaac aagtacaagc
     4381 acgagcgaat ccgattcaca aagcacatca acatatacaa gtcagtcaac aagccaaagt
     4441 gaatcaacat cgacatcaac gtcactaagc gattcaacaa gtatatctaa aagtacgagc
     4501 caatcaggtt caacaagcac atcagcatca ttaagtggtt cagagagtga atctgattca
     4561 caaagtatct caacaagtgc aagtgaatca acatcagaaa gtgcgtcaac atcactcagt
     4621 gactcaacaa gtacaagtaa ctcaggatca gcaagtacgt caacatcgct cagtaactca
     4681 gcaagcgcaa gtgaatccga ttcgtcgtca acatctttaa gtgattcaac atctgcgtca
     4741 atgcaaagca gtgaatccga ttcacaaagc acatcagcat cattaagtga ttcgctaagt
     4801 acatcaactt caaaccgcat gtcgaccatt gcaagtttat caacatcggt aagtacatca
     4861 gaatctggct caacatcaga aagtacaagt gaatccgatt caacatcaac atcattaagc
     4921 gattcacaaa gcacatcaag aagtacaagt gcatcaggat cagcaagtac atcaacatca
     4981 acaagtgact ctcgtagtac atcagcttca actagtactt cgatgcgtac aagtactagt
     5041 gattcacaaa gtatgtcgct ttcaacaagt acatcaacaa gtatgagtga ttcaacgtca
     5101 ttatctgata gtgtgagtga ttcaacatca gactcaacaa gtgcgagtac atctggttcg
     5161 atgagtgtat ctatatcgtt aagtgattcg acaagtacat caacatcggc tagtgaagta
     5221 atgagcgcaa gcatatctga ttcacaaagt atgtcagaat ctgtaaatga ttcagaaagt
     5281 gtaagtgaat ctaattctga aagtgactct aaatcgatga gtggctcaac aagtgtcagt
     5341 gattctggct cattgagcgt ctcaacgtca ttaagaaaat cagaaagtgt aagcgagtca
     5401 agttcattga gtggctcaca atcgatgagc gattcagtaa gcacaagcga ttcgtcatca
     5461 ttaagtgtat cgacgtcact aagaagttca gaaagcgtga gtgaatctga ttcattaagt
     5521 gattcaaaat caacaagtgg ttcgacttca acaagtacat ctggttcatt gagtacctca
     5581 acatcattaa gtggttcaga aagcgtaagc gagtctacct cgctaagtga ttcaatatca
     5641 atgagtgatt ctactagtac aagtgactcc gactcattaa gtggatcaat atctttaagt
     5701 ggttccacaa gtcttagcac ttcggattca ttaagtgatt caaaatcatt gagtagctcg
     5761 caaagtatga gtggatcaga atcaacgtca acaagtgtga gcgattcgca gtcaagctca
     5821 acaagtaata gtcaatttga ctctatgagc atcagtgcat cagaaagcga ctcaatgtct
     5881 acaagtgatt cgtctaacat cagtggatca aattcaacga gtacatcact ttcaacatct
     5941 gactcaatga gcggaagcgt atcagtttca acatcgacaa gtttaagtga ctcaatatca
     6001 ggttcaacaa gtgtaagtga ctcgagctca acaagcacat ctacatcatt aagtgattca
     6061 atgtcacaaa gccagtcaac aagtacaagt gcatctggtt ccttaagtac atcgatatca
     6121 acatcaatgt caatgagtgc tagtacatcg tcatcacaaa gcacatcggt gtcgacatca
     6181 ttatcaacat cagacagtat cagtgattct acttcaataa gtatcagtgg ttcacaaagt
     6241 acagtagaat cagaatcatt gagtacatca gattcagact cgacatcgac aagtacatcg
     6301 gactcaacaa gtggttcaac ttcaacaagc atatctgaat cattaagtac gtctggttca
     6361 ggttcaacga gcgtatctga ctcaacatca atgagtgaat ccgattcaac aagtgtttca
     6421 atgtcacaag acaaatccga ctcaacatca attagcgact cagaatcagt gtcaacaagc
     6481 acatcaacgt cattgagcac atccgattcg acaagcacat ccgaatcgct gagtacatct
     6541 atgtctggtt cacaaagcat ttctgactca acatcaacaa gtatgtccgg ctcaacaagt
     6601 acatctgaat ctaactcaat gcatccgtca gactcaatga gtatgcatca tactcacagc
     6661 acgagcacat ctcgcttatc aagtgaagca acaacgagca cgagtgaatc tcagtctaca
     6721 ttaagtgcaa catctgaagt gactaaacat aatggcacgc ctgcacaaag tgaaaaaaga
     6781 ttgccagata caggtgactc aataaaacaa aatggattac taggtggcgt tatgacatta
     6841 ttagttggtt taggtttaat gaagagaaag aaaaagaaag atgaaaatga tcaagatgat
     6901 tctcaagcat aaattgaatt tatagctggc taaaattaaa aagactgagg caccaatcac
     6961 tagaaaaatc gatataggat tttaataatt agtgatgaac ccttagtctt tttaatgttt
     7021 ttgatataaa agaggtgcaa catgttaaaa cttttacaac aatacgaat
//
LOCUS       DAHHSC010000123         6988 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.124, whole genome shotgun sequence.
ACCESSION   DAHHSC010000123 DAHHSC010000000
VERSION     DAHHSC010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 6988)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6988)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6988
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.124"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            7..426
                     /locus_tag="NBI57_001862"
     CDS             7..426
                     /locus_tag="NBI57_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HCD1822797.1"
                     /translation="MYKQLEKLITLTNNDLNLVNRRFGQRTDITSEQLELLRILFNYD
                     RLSQYDLTMKISREQSIVSRWIKKLVLKGYITSQQSSEDLRCKELILTDQARTLISQI
                     NNARCELIEARCQCLSEVELDNLNQLLDKLNQRRISL"
     gene            453..563
                     /locus_tag="NBI57_001863"
     CDS             453..563
                     /locus_tag="NBI57_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1822798.1"
                     /translation="MSLCTQQKLNHELQAKVAVIVKIDGKQSPLFLLVYV"
     gene            complement(633..4379)
                     /locus_tag="NBI57_001864"
     CDS             complement(633..4379)
                     /locus_tag="NBI57_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional autolysin"
                     /protein_id="HCD1822799.1"
                     /translation="MAKKFNYKLPSMVALTLVGSAVTAHQVQAAETTQDQTTNKNVLD
                     SNKVKATTEQAKAEVKNPTQNISGTQVYQDPAIVQPKTANNKTGNAQVSQKVDTAQVN
                     GDTRANQSATTNNTQPVAKSTSTTAPKTNTNVTNAGYSLVDDEDDNSEHQINPELIKS
                     AAKPAALETQYKAAAPKAKTEATPKVTTFSASAQPRSVAATPKTSLPKYKPQVNSSIN
                     DYIRKNNLKAPKIEEDYTSYFPKYAYRNGVGRPEGIVVHDTANDRSTINGEISYMKNN
                     YQNAFVHAFVDGDRIIETAPTDYLSWGVGAVGNPRFINVEIVHTHDYASFARSMNNYA
                     DYAATQLQYYGLKPDSAEYDGNGTVWTHYAVSKYLGGTDHADPHGYLRSHNYSYDQLY
                     DLINEKYLIKMGKVAPWGTQFTTTPTTPSKPTTPSKPSTGKLTVAANNGVAQIKPTNS
                     GLYTTVYDKTGKATNEVQKTFAVSKTATLGNQKFYLVQDYNSGNKFGWVKEGDVVYNT
                     AKSPVNVNQSYSIKSGTKLYTVPWGTSKQVAGSVSGSGNQTFKASKQQQIDKSIYLYG
                     SVNGKSGWVSKAYLVDTAKPTPTPIPKPSTPTTNNKLTVSSLNGVAQINAKNNGLFTT
                     VYDKTGKPTKEVQKTFAVTKEASLGGNKFYLVKDYNSPTLIGWVKQGDVIYNNAKSPV
                     NVMQTYTVKPGTKLYSVPWGTYKQEAGAVSGTGNQTFKATKQQQIDKSIYLFGTVNGK
                     SGWVSKAYLAVPAAPKKAVAQPKTAVKAYTVTKPQTTQTVSKIAQVKPNNTGIRASVY
                     EKTAKNGAKYADRTFYVTKERAHGNETYVLLNNTSHNIPLGWFNVKDLNVQNLGKEVK
                     TTQKYTVNKSNNGLSMVPWGTKNQVILTGNNIAQGTFNATKQVSVGKDVYLYGTINNR
                     TGWVNAKDLTAPTAVKPTTSAAKDYNYTYVIKNGNGYYYVTPNSDTAKYSLKAFNEQP
                     FAVVKEQVINGQTWYYGKLSNGKLAWIKSTDLAKELIKYNQTGMTLNQVAQIQAGLQY
                     KPQVQRVPGKWTDANFNDVKHAMDTKRLAQDPALKYQFLRLDQPQNISIDKINQFLKG
                     KGVLENQGAAFNKAAQMYGINEVYLISHALLETGNGTSQLAKGADVVNNKVVTNSNTK
                     YHNVFGIAAYDNDPLREGIKYAKQAGWDTVSKAIVGGAKFIGNSYVKAGQNTLYKMRW
                     NPAHPGTHQYATDVDWANINAKIIKGYYDKIGEVGKYFDIPQYK"
     gene            complement(4607..5041)
                     /locus_tag="NBI57_001865"
     CDS             complement(4607..5041)
                     /locus_tag="NBI57_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCD1822800.1"
                     /translation="MFSKVNNQKMLEDCFYIRKKVFVEEQGVPEESEIDEYESESIHL
                     IGYDNGQPVATARIRPINETTVKIERVAVMKSHRGQGMGRMLMQAVESLAKDEGFYVA
                     TMNAQCHAIPFYESLNFKMRGNIFLEEGIEHIEMTKKLTSLN"
     gene            complement(5196..5666)
                     /locus_tag="NBI57_001866"
     CDS             complement(5196..5666)
                     /locus_tag="NBI57_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499548.1"
                     /note="Essential protein for resistance to osmotic stress
                     from salt; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotic stress response protein"
                     /protein_id="HCD1822801.1"
                     /translation="MSRKTYEKIANINGMFNMLEQQIIHSQDMAHFRSEFFYVNHEHR
                     ENYEALLIYYKNSIDNPIVDGACYILALPEIFNSVDVFESELPFSWVYDENGITETMK
                     SLSIPLQYLVAAALEVTDVNIFKPSGFTMGMNNWNIAQMRIFWQYTAIIRKEAL"
     gene            complement(5714..6931)
                     /locus_tag="NBI57_001867"
     CDS             complement(5714..6931)
                     /locus_tag="NBI57_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyisoprenyl-teichoic acid--peptidoglycan
                     teichoic acid transferase"
                     /protein_id="HCD1822802.1"
                     /translation="MNKFLKYFLILLALVLIVVPIVFATLLFKTSQDAFESSQDSKNA
                     NRQSNLRDNKVNPEEQPISILFLGIDDNDGRRKKGQDAEHSRSDAMILTTFNQSKHQI
                     RMLSIPRDTISYIPKVGYYDKITHAHAYGGPIAAMDSVEATMNVPVDYYVRVNMKAFV
                     EAVNELGGIYYDVPYDLNEPNTDDTGKIKIKKGYQKLNGDEALAVARTRHHDSDLKRG
                     QRQMELIKILFQKAQEVDSIDKLDNVIQIVGKNAKHNLTNSEIKALAKMYLTNDVEIK
                     TAQLKGKDDMLNGIYYYHPSVESIQKYANLLRKDLELSPINDKNDFLDQRVINHYGSL
                     IPLTPLDSSLLRKEQNDTTDKDKTSNENNDSTNNSDSSNQQQPATDQNLNQNQGGTQQ
                     APQASNNQNGVVN"
BASE COUNT         2047 a         1150 c         1165 g         2626 t
ORIGIN      
        1 atcaacatgt ataaacaact tgaaaaactt attacactga ctaacaatga cttaaactta
       61 gtgaatagaa gatttggaca acgcacggat atcacatctg aacagctaga acttctccgt
      121 attttattta attacgatcg cttatcacag tatgatttga cgatgaagat tagcagggaa
      181 caatctatag tttcaaggtg gattaagaaa ttagttttga aaggttacat cacaagtcaa
      241 caatctagcg aagatttaag atgtaaagaa ttaattttga ctgatcaagc acgtacatta
      301 atttcacaaa taaataatgc acgttgcgaa ttgattgaag caagatgtca atgtttatcg
      361 gaagtcgaat tagacaattt aaatcaatta cttgataagt taaatcaacg acgcatatcg
      421 ttgtaacacg acaatagcaa acacataatt tagtgtcatt gtgcacacaa caaaaattga
      481 atcatgaatt acaagcaaaa gtagcggtga ttgttaaaat tgatggtaaa caatcaccgc
      541 tatttttgct tgtgtatgta taaaaaaggg atcaaaggtc atcccccatg attgatagtg
      601 ggggatgact tttgatccta tgttcatgtt gcttatttat attgtgggat gtcgaagtat
      661 ttgccgactt cgccaatttt atcatagtag cctttgatga ttttagcatt gatgttagcc
      721 caatctacat ctgtagcata ttggtgtgtt cctggatgtg caggattcca tctcattttg
      781 taaagtgtat tttgaccagc ttttacatat gagttgccga tgaatttagc accaccaacg
      841 attgcttttg atactgtgtc ccaaccagct tgtttagcat atttaatacc ttcacgtaaa
      901 ggatcgttat catatgcagc aataccaaat acgttatggt atttcgtgtt tgagttagtt
      961 acaactttgt tgttcactac atctgcacct ttcgctaatt gagaagtacc gttacctgtt
     1021 tctaataggg catgtgagat aagataaact tcattaatgc catacatttg agcagctttg
     1081 ttaaatgcag caccttggtt ttctaataca cctttacctt ttaagaattg attaatttta
     1141 tcaatagaaa tattttgtgg ttggtctaag cgtaagaatt gatattttaa tgctggatct
     1201 tgagctaaac gcttcgtatc cattgcatgc ttaacatcat taaagttagc atctgtccac
     1261 ttacctggta cacgttgtac ttgtggttta tattgtaaac cagcttgtat ttgagcaact
     1321 tggtttaatg tcatacctgt ttgattatac ttaattaatt ctttagctaa atcagttgat
     1381 ttaatccatg ctaatttacc gttagataat ttaccatagt accaagtttg tccattaatg
     1441 acttgttctt taacaactgc gaatggttgt tcattaaatg cttttaatga gtatttagct
     1501 gtatcagaat ttggtgttac atagtaataa ccattaccat ttttaattac ataagtgtag
     1561 ttataatctt tggcagctga tgtagttggt ttcacagcag ttggtgcagt taaatctttt
     1621 gcatttaccc aaccagtgcg gttattaata gtaccgtata aataaacatc tttgcctaca
     1681 gatacttgtt tcgttgcatt aaatgtacct tgagcaatgt tattgcctgt taaaatgact
     1741 tggtttttag taccccaagg aaccattgat aagccgttat ttgatttatt aacagtatat
     1801 ttttgagtcg ttttaacttc tttgcctagg ttttgaacat ttaagtcttt tacattgaac
     1861 caacctaatg ggatgttatg gcttgtattg tttaataata catacgtttc attaccatga
     1921 gcacgctctt ttgttacata gaacgtacgg tctgcatatt tcgcaccgtt tttcgctgtt
     1981 ttttcataaa cagaagcacg aataccagtg ttgtttggtt taacttgagc aatcttgcta
     2041 actgtttgag tcgtttgtgg tttagtaaca gtataagctt ttacagctgt ttttggttgt
     2101 gctactgctt ttttaggtgc agcaggtaca gctaaatatg ctttacttac ccaaccagat
     2161 ttaccattta cagttccaaa taaatagata gatttatcaa tttgttgttg cttagtcgct
     2221 ttaaaagttt ggttacctgt accagaaact gcaccagctt cttgtttata agtgccccaa
     2281 ggtactgaat ataatttagt gcctggtttt actgtatatg tttgcattac atttacaggt
     2341 gattttgcat tgttataaat aacgtcacct tgtttaaccc aaccaattaa agttggacta
     2401 ttgtaatctt taactaagta gaatttgttt ccacctaaac ttgcttcttt tgttacagca
     2461 aatgtttttt gaacttcttt cgttggctta ccagttttgt cataaactgt agtgaataag
     2521 ccattgtttt tagcattaat ttgagcaaca ccgtttaatg atgaaactgt taatttatta
     2581 tttgttgtag gtgttgatgg cttaggtatt ggtgtaggcg taggtttagc agtatcaact
     2641 aaatatgctt tacttaccca accagattta ccattcacag agccatataa ataaattgat
     2701 ttatcaattt gttgttgctt tgaagcctta aatgtttggt ttccagagcc agacacgcta
     2761 ccagcaactt gtttagatgt accccaaggt actgtataaa gtttcgtacc agatttgatt
     2821 gaatatgatt gatttacatt tacaggtgat ttagctgtgt tgtaaaccac atcgccttct
     2881 ttaacccaac caaatttatt accagaattg taatcttgaa caagatagaa tttttgatta
     2941 cctaatgtag ctgttttaga tacagcaaat gttttttgaa cttcattagt tgctttacca
     3001 gttttgtcgt aaacagtagt atataaacca ctatttgttg gtttgatttg tgcgacacca
     3061 ttgtttgctg caactgttaa tttaccagtt gatggtttcg acggtgttgt tggttttgat
     3121 ggtgtagtag gggtagttgt aaattgcgta ccccatggcg ccactttacc catttttatt
     3181 aaatattttt cattaattaa gtcatataat tgatcataac tataattatg acttcttaaa
     3241 tatccatgtg gatcggcatg gtccgtacca cctaaatatt tacttacagc gtagtgagtc
     3301 catactgtac catttccatc atactcagca ctgtctggtt ttaaaccata atattgtaat
     3361 tgtgtagctg catagtcagc atagttattc attgaacgtg caaatgaagc atagtcgtgt
     3421 gtgtgtacga tttcaacatt gatgaatcta gggttaccga ctgcaccgac accccaagat
     3481 aagtaatccg ttggtgctgt ttcgattata cgatccccat caacaaatgc atgtacgaat
     3541 gcgttttgat agttattttt catataacta atttcaccat ttatcgtcga acgatcatta
     3601 gctgtatcat gaactacgat accttcagga cgacctacgc cgttacggta tgcgtattta
     3661 gggaagtaag atgtataatc ttcttcaatt ttaggtgctt ttaagttatt tttacgaatg
     3721 taatcgttaa ttgaagagtt tacttgtggt ttatattttg gcaaactcgt ttttggtgtt
     3781 gcagcaactg atcttggttg tgctgaagcg ctaaaagtag ttactttagg tgtcgcttca
     3841 gttttagctt taggtgctgc ggctttatat tgcgtttcaa gagctgcagg tttagcagct
     3901 gatttaatta attctggatt aatttgatgt tctgaattat catcttcatc atcaactaaa
     3961 ctataaccag catttgtaac attagtgtta gttttaggtg ctgtagtgct tgttgacttt
     4021 gcaacaggct gcgtattatt tgtagtcgct gattgattag cacgagtgtc accatttact
     4081 tgtgcagtat caactttttg acttacttga gcattgcctg ttttgttatt tgctgttttt
     4141 ggttggacaa tagcagggtc ttgatatact tgagtgccag aaatgttttg cgttggattt
     4201 tttacctcag cttttgcttg ttcagtagtt gctttaactt tattactatc taaaacattt
     4261 ttattagtag tttgatcttg tgtcgtctca gctgcttgaa cttgatgtgc agtgactgct
     4321 gaacctacaa gcgttaatgc aaccattgat ggtagtttgt aattgaattt tttcgccatt
     4381 ctatttatta ctcctaacat ttattaatta ttactaacat tatagtacct gttttatata
     4441 cctgtgcgta tttaaccaat ttgtaatata ctcttaattt acattttcac tgtaaaaaaa
     4501 tgaaaaaaat tttgaatctc aatggtgacg tgacaaatca aatagaattg atgtcaattt
     4561 aagaattggt ttattacaaa tgtttctaaa atagatacaa ctttttttaa ttaagcgagg
     4621 ttaacttttt tgtcatttca atatgctcga tgccttcctc aagaaatata ttacctctca
     4681 ttttaaagtt taaactttca taaaatggga tagcatgaca ttgggcattc atagtagcta
     4741 cgtaaaaacc ttcatcttta gctaatgatt ctacagcttg cataagcatt ctacccattc
     4801 cttgtccacg atgtgatttc atcacagcta ctcgttctat tttgacagtt gtttcattaa
     4861 tagggcgtat tcgagcagtg gcaactggct gtccattatc atatccaatg aggtgaatag
     4921 attcagattc atattcatca atttcacttt cctcagggac gccttgttct tctacaaaca
     4981 ctttctttct tatatagaag caatcttcta acatcttttg attgtttact tttgaaaaca
     5041 taaaattcct ccacatccta acaaaatcat ttacatttaa tttactaata ataacataga
     5101 ataacgattg ttattttcct caaaaataat tagacataaa aaagctataa agatgattca
     5161 caagaatcat ctttatagct aattaattat taatgttata gtgcttcttt tctaataatt
     5221 gctgtatatt gccaaaagat tcgcatttga gcaatattcc aattattcat tcccattgta
     5281 aatcctgaag gcttaaatat attcacatca gttacttcta atgctgctgc aactaaatat
     5341 tgtaatggaa tgcttagtga tttcattgtt tcggtaatgc cattttcatc atatacccat
     5401 gaaaatggta actctgattc gaaaacatca acactattga aaatttcagg tagggctaaa
     5461 atataacatg caccatctac aataggattg tcgatactat ttttgtaata aattaggagt
     5521 gcttcatagt tttctcgatg ctcatgattg acgtaaaaaa attcacttct aaaatgagcc
     5581 atatcttggc tatgaatgat ttgttgttct aacatattaa acatgccatt tatatttgca
     5641 atcttttcgt atgtttttct actcatgtat gatgctcctt tcataaatag taaatgttaa
     5701 tcgtcatata atattaattt acaacaccat tttggttatt tgaagcttgt ggcgcttgtt
     5761 gtgtgccacc ttgattttga tttaagtttt gatctgtagc aggttgttgt tgattgctgg
     5821 aatcactgtt attagttgaa tcattgttct cgttagatgt cttatcttta tctgtcgtat
     5881 cattttgttc ttttctcaat aaactactat ctaaaggcgt taacggtatt aatgaaccat
     5941 aatgattaat gacacgttga tctaagaaat catttttatc attaataggt gataattcta
     6001 agtctttacg aagtaagttt gcatattttt gaatgctttc aacacttgga tgataatagt
     6061 aaataccatt taacatatca tctttacctt ttaattgcgc agttttaatt tcaacatcat
     6121 ttgttaagta catttttgct aaagctttaa tttcagagtt agttaaatta tgctttgcat
     6181 ttttgcctac aatttgaatc acgttatcaa gtttatcaat agaatcaact tcctgtgctt
     6241 tttggaataa aatcttaatt aattccattt gacgttgtcc acgttttaag tctgaatcat
     6301 gatgtctagt tctagcaact gctaaagcct catcaccatt taatttttgg tacccttttt
     6361 taattttaat cttaccagta tcatctgtgt taggttcatt taagtcgtat ggcacatcat
     6421 agtatatgcc accaagctca tttacagcct cgacaaatgc tttcatattg actctcacat
     6481 aataatcaac aggtacattc atggtagctt ctaccgaatc cattgcggca attggaccac
     6541 catatgcatg tgcatgggta atcttatcgt aatagccaac tttaggaatg tagctgatag
     6601 tatcacgtgg aatactaagc attctaattt gatgttttga ttgattaaaa gtagttaaaa
     6661 tcatagcgtc tgatctagag tgttcagcat cctgtccttt ttttcttctt ccatcgttat
     6721 catcgatacc taagaaaaga atagagatag gttgttcttc gggattgact ttattatctc
     6781 ttaagttgga ttgacgatta gcatttttgc tgtcttgaga agattcgaat gcatcttggg
     6841 acgttttaaa aagtaacgta gcgaagacta ttggaacaac aatgagaacc aatgctagaa
     6901 ggatcaaaaa gtattttaaa aatttattca tgattgatgc tcctatatta aatttttgtt
     6961 aaatcataat cataatttta aaatataa
//
LOCUS       DAHHSC010000124         6953 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.125, whole genome shotgun sequence.
ACCESSION   DAHHSC010000124 DAHHSC010000000
VERSION     DAHHSC010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 6953)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6953)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6953
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.125"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            complement(19..1146)
                     /locus_tag="NBI57_001868"
     CDS             complement(19..1146)
                     /locus_tag="NBI57_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HCD1822803.1"
                     /translation="MKSKYEPLFDKVELPNGVELRNRFVLAPLTHISSNDDGTISDVE
                     LPYIEKRSQDVGITINAASNVSDVGKAFPGQPSIAHDSDIEGLKRLATAMKKNGAKAL
                     VQIHHGGAQALPELIPDGDVVAPSPISLKSFGQKQEHSAREMTNEEIEQAIKDFGEAT
                     RRAIEAGFDGVEIHGANHYLIHQFVSPYYNRRNDVWANQYKFPVAVIEEVLKAKEAYG
                     NKDFIVGYRLSPEEAESPGITMEITEELVNKISHMPIDYIHVSMMDTHATTREGKYAG
                     QERLPLIHKWINGRMPLIGIGSIFTADEALDAVENVGVDLVAIGRELLLDYQFVEKIK
                     DGREDEIINYFDPEREDNHHLTPNLWHQFNEGFYPLPRKDK"
     gene            complement(1508..1885)
                     /locus_tag="NBI57_001869"
     CDS             complement(1508..1885)
                     /locus_tag="NBI57_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001067296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HCD1822804.1"
                     /translation="MNNYKIGQHIKVRVTGIQPYGAFVETPNHTEGLIHISEIMDDYV
                     HNLKKFLSEGQIVKAKILSIDDEGKLNLSLKDNDYFKNYERKKEKQSVLDEIRETEKY
                     GFQTLKERLPIWIKQSKRAIRND"
     gene            complement(2300..2893)
                     /locus_tag="NBI57_001870"
     CDS             complement(2300..2893)
                     /locus_tag="NBI57_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HCD1822805.1"
                     /translation="MANYPQLNKEVQQGEIKVVMHTNKGDMTFKLFPNIAPKTVENFV
                     THAKNGYYDGITFHRVINDFMIQGGDPTATGMGGESIYGGAFEDEFSLNAFNLYGALS
                     MANSGPNTNGSQFFIVQMKEVPQNMLSQLADGGWPQPIVDAYGEKGGTPWLDQKHTVF
                     GQIIDGETTLEDIANTKVGPQDKPLHDVVIESIDVEE"
     gene            2957..3340
                     /locus_tag="NBI57_001871"
     CDS             2957..3340
                     /locus_tag="NBI57_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase-associated lipoprotein B"
                     /protein_id="HCD1822806.1"
                     /translation="MKLYRFSHKTGSYGVTIVEENGDDVLVKVEQVIKHPKQGDLHNP
                     TETEGVFFHERKALSHFEKRYTKRSQLRDFNVDVLRYEDSLQQAITDLETKLKAQQTK
                     HAEMSLASLARLKEDYSLQYKENFK"
     gene            3471..5876
                     /gene="mnhA1"
                     /locus_tag="NBI57_001872"
     CDS             3471..5876
                     /gene="mnhA1"
                     /locus_tag="NBI57_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh1 subunit A"
                     /protein_id="HCD1822807.1"
                     /translation="MSLLHIAVILPLIFALIIPILYRFFKRIHLGWFVLPVPIVIFIY
                     MLTLIKTTMSGNTVMKTLNWMPHFGMNFDLYLDGLGLLFSLLISGIGSLVVLYSIGYL
                     SKSEQLGNFYCYLLLFMGAMLGVVLSDNVIILYLFWELTSFSSFLLISFWRERQASIY
                     GAQKSLIITVFGGLSLLGGIILLAIPTQSFSIQYMIQHASEIQNSPFFIFAMILIMIG
                     AFTKSAQFPFYIWLPDAMEAPTPVSAYLHSATMVKAGLYLIARMTPIFAASQGWVWTV
                     TLVGLITLFWASLNATKQQDLKGILAFSTVSQLGMIMAMLGIGAISYHYQGDDSKIYA
                     AAFTAAIFHLINHATFKGALFMITGAVDHSTGTRDVKKLGGLLTIMPISFTITVITAL
                     SMAGVPPFNGFLSKESFLETTFTASQANLFSVDTLGYLFPIIGIVGSVFTFVYSIKFI
                     MHIFFGQYKPEQLPKKAHEVSILMLLSPAILATLVIVLGLFPGILTNSIIEPATSSIN
                     HTVIDDVEFHMFHGLTPAFLSTLVIYILGILLIVTFSYWVKLLQRQPGKLTFNYWYNR
                     SANVIPNYSEKMTNSYVTDYSRNNLVIIFGALILLTFVTIFSVPFNINFKDVSPIRIF
                     EVCIVILLLSAAFLILFAKSRLFSIIMLSAVGYAVSVLFIFFKAPDLALTQFVVESIS
                     TALFLLCFYHLPNLNRYNEKRSFQLTNALIAGGVGLSVIIIGLIAYGNRHFESISKFY
                     QEHVYDLAHGKNMVNVILVDFRGMDTLFESSVLGIAGLAVYTMIKLRKKRQTQGNEVK
                     NHE"
     gene            5869..6297
                     /gene="mnhB1"
                     /locus_tag="NBI57_001873"
     CDS             5869..6297
                     /gene="mnhB1"
                     /locus_tag="NBI57_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh1 subunit B"
                     /protein_id="HCD1822808.1"
                     /translation="MNRQQNDLILQFAAVIIFFMVMVFGFSLFLAGHYTPGGGFVGGL
                     LFASSLVIITIAFDIETMRKIFPLDFKILIGIGLVFCIATPIASWFLGKNFFTHVTFD
                     IPLFILEPVHMTTAVFFDFGVLCAVVGTVMTIIISIGENE"
     gene            6297..6638
                     /gene="mnhC1"
                     /locus_tag="NBI57_001874"
     CDS             6297..6638
                     /gene="mnhC1"
                     /locus_tag="NBI57_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh1 subunit C"
                     /protein_id="HCD1822809.1"
                     /translation="MEIIMIFVSGILTAISVYLVLSKSLIRIVMGTTLLTHAANLFLI
                     TMGGLKHGTVPIYEANVKSYVDPIPQALILTAIVIAFATTAFFLVLAFRTYKELGTDN
                     VESMKGVPEDD"
     gene            6631..>6953
                     /locus_tag="NBI57_001875"
     CDS             6631..>6953
                     /locus_tag="NBI57_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499439.1"
                     /note="subunit D of antiporter complex involved in
                     resistance to high concentrations of Na+, K+, Li+ and/or
                     alkali; contains an oxidoreductase domain; catalyzes the
                     transfer of electrons from NADH to ubiquinone; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter subunit D"
                     /protein_id="HCD1822810.1"
                     /translation="MIESNMLVLTLVIPVITAILLVFIGKRPIIKRYVALGGTLLTLV
                     AAIINLANVVKHGPIRVELGSWKAPYSIVFVLDIFSALLIITSIIITAIVILYSYQTI
                     GIERE"
BASE COUNT         2124 a         1207 c         1100 g         2522 t
ORIGIN      
        1 gtggtaacag ttaaacactt atttatcttt acgtggtaat ggatagaatc cttcattaaa
       61 ttgatgccat aaattaggag ttaagtgatg attatcttct ctctctggat caaagtaatt
      121 aataatttca tcttcccgtc catctttaat tttttcaaca aattgataat ccagtagtag
      181 ctctctacca atggctacta agtcaacacc aacattttca actgcatcta aagcttcgtc
      241 agctgtgaaa attgaaccaa taccgataag tggcatacga ccatttatcc atttgtgaat
      301 taaaggtagt ctttcttgtc cagcgtattt accttcacgt gtcgttgcat gcgtatccat
      361 cattgaaaca tgaatatagt cgattggcat atggctaatt ttattaacga gttcctctgt
      421 aatttccatt gtgattcctg gagactccgc ttcctctgga gataatctgt atccaactat
      481 aaagtcttta ttgccatacg cttctttcgc tttaagcact tcttcaatca cagcgaccgg
      541 gaatttatat tgatttgccc atacatcatt tcttctatta tagtatggtg atacaaattg
      601 atgaattaag taatgattcg cgccatgtat ttcaacacca tcaaaccctg cttcaattgc
      661 acgtcgtgtt gcttcaccaa aatccttgat tgcttgttca atctcttcat tcgtcatttc
      721 tctagcacta tgttcttgtt tctgaccgaa actttttaaa gaaattggac ttggtgctac
      781 gacgtctcca tcaggtatta attcaggcaa tgcttgtgca ccaccatgat gtatttgtac
      841 gagtgctttg gcaccgtttt tcttcattgc tgtagctaat cgttttagtc cttcaatatc
      901 actgtcatgc gcgattgacg gctgtcctgg aaatgctttt ccgacatcac tcacattact
      961 cgcagcatta attgtaatac caacatcttg tgaacgcttt tcaatataag gaagttctac
     1021 atctgaaata gtaccatcat catttgaaga aatatgtgtt aaaggggcta acacaaatcg
     1081 atttctcaac tctactccat ttggtaattc tactttatca aacaatggtt cgtatttact
     1141 tttcattact taattcctcc ttaaaattat tgagttgact ataacatgta attttttcat
     1201 tctccaatta tctgtttata tagttgcaat tcgtttaata ctttaagttg tagttctcat
     1261 ttctaaaata aataaaaaaa acaaatccac atacaatatt gcaaatcaac gattctatct
     1321 aacaaagata atggatatgt caccttaaac catttgtacg tgtaaataga atcaatcata
     1381 ttacgttatc gtagacaaaa agagattagt atctaataaa atttaataga tactaatctc
     1441 ttttatagtc taagaaactt tcatttcaga ccctttgtat gattttcggt acgatttatc
     1501 tgttccttta gtcgtttcga attgctcgct ttgactgttt tatccagatt ggtaagcgtt
     1561 ctttaagtgt ttgaaaccca tatttttctg tttctctgat ttcatctaat actgattgtt
     1621 tttccttctt acgctcataa tttttgaagt aatcattatc ctttaatgat agattaagct
     1681 ttccttcatc atctatagac aaaattttag ctttaacaat ttggccttct gatagaaatt
     1741 tcttcaaatt atgaacgtag tcatccataa tttctgatat atgaatcagt ccttcagtat
     1801 gattaggggt ctcaacaaac gcaccgtatg gttgaatacc agttacacgc accttgatat
     1861 gttggccaat tttgtagtta ttcaacttaa taacccctta tttcgtttac ttaacactat
     1921 tatcatagca caaatcgaaa tggtggacta attaaaacat caaatcatac gaatattatt
     1981 ttataaatga ataagaattc tacaaacttg gatttacaaa taaaattagc aacaaactcc
     2041 tatctaaatc aatatgatat ccagctactt tgttttcaat ttattaacta tttgaatagc
     2101 aattctcttt aaaaacataa aaagcaacct caactattat ctttagaata cacatctatt
     2161 taaaaactgt gctttcttga taaatatatt gagattgctc atcatgttag tttatttgtt
     2221 tcgttggaat atatactaat ccattcataa attagcttta tccgagttta aaatgttggt
     2281 agttaattat gtttagctat tattcttcaa catcaataga ttcaattaca acatcatgaa
     2341 gtggtttgtc ttgtggtccc acttttgtat ttgcaatatc ttctaaagta gtttcaccat
     2401 caatgatttg accgaatact gtatgttttt gatctaacca tggtgtacca cccttttcgc
     2461 catatgcatc aacgattggt tgaggccagc caccatctgc aagttgactt aacatatttt
     2521 gaggtacttc tttcatttga acaatgaaaa attgtgaacc attagtatta ggtcctgagt
     2581 tagccattga taatgcgcca tataagttaa atgcatttaa tgaaaattca tcttcaaaag
     2641 caccgccata aatactttcg ccacccatac cagtagctgt tggatcgcca ccttgaatca
     2701 tgaagtcatt aatgacacgg tggaatgtta ttccatcata ataaccattt tttgcatgtg
     2761 tcacaaaatt ttcaactgtt tttggtgcaa tatttggaaa taatttgaat gtcatgtcac
     2821 ctttatttgt gtgcataacc actttaattt caccttgttg tacttctttg tttaactgtg
     2881 gatagttagc catttatatt ctccattcat gttatgatac atatatatta acatactttt
     2941 aagggaggaa aatagcatga agctatatcg attttcacac aaaacaggta gctatggtgt
     3001 cacgattgtt gaagaaaatg gagacgatgt ccttgttaaa gttgaacaag ttatcaaaca
     3061 cccgaaacaa ggtgatttac ataaccctac tgaaactgaa ggtgtatttt tccatgaacg
     3121 caaagcatta agccattttg aaaaacgcta tacgaaacgt tcacaattac gcgacttcaa
     3181 tgttgatgta ttgcgctatg aagattcact tcaacaagca attacagatt tagaaactaa
     3241 acttaaagct cagcaaacaa aacatgctga aatgtcttta gctagtttag cacgcttaaa
     3301 agaagattat tcacttcaat acaaagaaaa ttttaaataa aaaatgacgt gtttcttctt
     3361 atttaaatac ttctgaaaat aaagcacaac aaggagtgtg tacgtttttc agaactatat
     3421 cattcatgta taatgaataa cataaatatt ttttaaagga ggacaacata gtgagtttgt
     3481 tacatattgc ggtgatatta ccgttaatat tcgcactcat tattcctatc ctgtatagat
     3541 tttttaaacg tatacattta ggatggttcg tattacctgt ccctatcgtc atatttattt
     3601 atatgcttac attaattaaa acgacgatgt caggtaatac tgtaatgaaa accttaaatt
     3661 ggatgcctca ttttggtatg aactttgatt tatatcttga tggcttaggt ttactcttta
     3721 gtttgcttat ttcaggaatc ggcagtttag tagtattata ttcaatcggc tacttaagta
     3781 agtcagaaca actagggaat ttttattgtt atttattact atttatgggt gcaatgttag
     3841 gtgtcgtact ttcagataat gttattattc tatatttatt ctgggagcta acgtcatttt
     3901 ctagtttttt acttatttca ttctggcgcg aacgacaagc atctatatat ggtgctcaaa
     3961 aatcgctcat aatcacagta tttggtggtc tatcattatt aggtggtatc atcttactcg
     4021 ctataccaac acaatcattt agcattcagt acatgattca acatgcttct gaaattcaaa
     4081 atagcccatt cttcattttt gcgatgattt taatcatgat tggtgctttt acaaaatctg
     4141 ctcagtttcc attttatatt tggctaccag atgcaatgga agcacctaca ccagtcagtg
     4201 cttatcttca ttcagcaaca atggttaaag cagggttata tttaatcgct cgaatgacac
     4261 caatcttcgc agcatcgcaa ggttgggtat ggactgtcac actcgttggt ttaattacgt
     4321 tattctgggc ttcgctaaac gcaacaaaac aacaagactt gaaaggtata ctggcatttt
     4381 caacagtatc ccaattaggt atgattatgg cgatgcttgg aatcggtgcc attagttatc
     4441 actatcaagg cgatgatagc aaaatatatg ctgctgcatt tacggctgca atattccatt
     4501 taatcaacca tgcaacattt aaaggtgccc tatttatgat tacaggtgca gtggatcatt
     4561 ctactggtac acgtgatgtt aagaaattag gtggcttact aacaattatg cctatttcat
     4621 ttacaatcac tgttattaca gcattaagta tggctggtgt accaccgttc aatggtttct
     4681 tatcaaaaga atcattttta gaaacaacat ttacagcaag ccaagcaaat ttattcagtg
     4741 ttgatacatt aggctaccta ttcccaatca ttggtatagt gggcagtgtc ttcacatttg
     4801 tatattcaat taaatttata atgcatatat ttttcggaca atataaacct gaacaattac
     4861 ctaaaaaagc acacgaagta tcaatattaa tgcttttatc accagctatt ttagctacat
     4921 tagtaattgt attgggttta ttccctggca ttttaactaa ttcgattatt gaaccagcta
     4981 catcttcgat taatcatacg gtaattgatg atgttgaatt ccatatgttc catggattaa
     5041 cgcctgcctt tttatcaaca ttagtgattt atattttagg tatcttacta attgtgacat
     5101 ttagttattg ggtgaaattg ttgcaacgac aacctggtaa actaacattc aattattggt
     5161 acaacagaag tgcaaatgtt attccaaact actctgaaaa gatgacgaat agctatgtta
     5221 ccgattattc acgtaataat ttagttatca tttttggtgc attaatttta ttaacatttg
     5281 tcactatatt ctcagtgcct ttcaacatta attttaaaga cgttagtccg attagaattt
     5341 tcgaagtttg tatcgtaatt ttattattaa gtgctgcctt tttaattctt ttcgctaaat
     5401 caagattatt tagcattatt atgttaagtg cagttggata tgctgtgtct gtattgttta
     5461 tattctttaa agcacccgac ttagcattga cacagtttgt cgttgaatct atttcaacag
     5521 cattgttctt attatgcttc taccacttac caaatttaaa tcgttataat gaaaagcgct
     5581 cattccagct tactaatgct ttaattgctg gtggtgttgg tttgtccgtc attattatcg
     5641 gtttaattgc atacggaaat agacattttg aatcgatatc taagttttat caagagcacg
     5701 tttatgattt agctcacggt aaaaacatgg taaacgtcat acttgttgat ttccgtggta
     5761 tggatacttt attcgagtca tctgtgcttg gtatcgcagg attagctgta tatacaatga
     5821 ttaaattacg taaaaagcgt caaacgcaag gtaacgaggt gaaaaatcat gaatagacaa
     5881 caaaatgatc taattttgca atttgctgca gttattatct ttttcatggt aatggtcttt
     5941 ggtttttcac ttttcttagc aggacattat acacctggtg gcggtttcgt cggcggatta
     6001 ttattcgcaa gttcacttgt tatcattacg attgcatttg atatagaaac aatgcgtaaa
     6061 attttcccat tagattttaa aatcttgata ggtatcggat tagttttttg tattgcgacg
     6121 cctatcgcaa gttggttttt aggcaaaaat ttctttacgc atgtcacatt tgatataccg
     6181 ttatttatat tagaacctgt tcatatgaca acagcagtat tctttgattt cggtgtttta
     6241 tgtgcagtag ttggtactgt catgacaatt attatttcga ttggagagaa cgaatagtgg
     6301 aaattattat gatttttgtt agtggtattc tcacagcaat tagtgtctat ctcgttttgt
     6361 ctaaaagtct gatacgaatt gttatgggaa ctacactatt aacacatgca gcaaacttat
     6421 ttttaataac tatgggcgga cttaaacatg gtactgttcc aatttatgaa gcgaacgtaa
     6481 aaagctatgt tgatcctatc ccgcaagcac ttattttaac agcaatcgtt atcgcctttg
     6541 cgacaacagc ctttttctta gtattagcat ttagaacata taaagaatta ggcacagata
     6601 acgttgagag tatgaaagga gttccagaag atgattgaaa gtaatatgct tgtgttaacg
     6661 ctagttattc cagtaattac agccatatta cttgtcttta ttggaaagcg tcctattata
     6721 aagcgttatg tcgcactagg cggtacatta ttaaccttgg tcgcggcaat tatcaattta
     6781 gcaaatgttg ttaaacatgg accaatacgc gtagagctag gttcatggaa agcaccatac
     6841 agtattgtct ttgtattaga catattcagt gccctactaa taattactag tattattatt
     6901 acagctattg tgattttata ttcataccaa acaattggta ttgaacgtga aag
//
LOCUS       DAHHSC010000125         6878 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.126, whole genome shotgun sequence.
ACCESSION   DAHHSC010000125 DAHHSC010000000
VERSION     DAHHSC010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 6878)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6878)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6878
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.126"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            complement(110..1483)
                     /locus_tag="NBI57_001876"
     CDS             complement(110..1483)
                     /locus_tag="NBI57_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EVE domain-containing protein"
                     /protein_id="HCD1822811.1"
                     /translation="MTAETNYFWLNCGYNRWNHNEPLVGQTALFESGAHFNPSQGFRA
                     FKKAKVGDQVIFYQVQTDTGLLGCGEIISVETGAQNKIRVQFRFNEQLKPLTADYLKR
                     SEALEFRMSNMKETLFNQITAEEFDLISGLGKGEIKIPRYFFLAETEEFEPGNQYTIY
                     THTYNGIKRNGYHFYTQLEEGDNIIFYNRTKNQSVVGIGEVSKHIHEKPPIPGRTNST
                     VIEVSYEKDITPITLSTLNKHPKLKNLYFLQENAKQAIASMSQAQYDAIIEMSDNNGL
                     KSPFEMVKKPDMLESEKEEALKPFILLVVDRKEEGLKAANDLLQKANANPVITTGHPD
                     FSEDMLYGKYLPNETGALYYREGFITQLMPKKDKSYLVIDNFNRIDTDIFQTYINVLE
                     GYEVTLPRYNKDGNMIKWSRQKDSFYYFNPNWHIVGITYDSLEEIKEKYSEQFLKYTR
                     IVKVKHD"
     gene            complement(2155..2385)
                     /locus_tag="NBI57_001877"
     CDS             complement(2155..2385)
                     /locus_tag="NBI57_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1822812.1"
                     /translation="MKQFLYIALVCGVIAGLGAFLHIPQYPSMTIPRIVAILGIISAM
                     LTFKDKQISASLKFSALLINVLPLCGTFVASN"
     gene            complement(2416..3354)
                     /locus_tag="NBI57_001878"
     CDS             complement(2416..3354)
                     /locus_tag="NBI57_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001620169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I mannose-6-phosphate isomerase"
                     /protein_id="HCD1822813.1"
                     /translation="MPLFLQPILKTKLWGGQRLSEFGYQLDNDTTGECWCVSAHPNGT
                     SEIINGPYQGQTLDRIWSEHRELFGDFPSKDFPLLTKIVDARESLSIHVHPDNSYAYE
                     HENGQYGKSECWYIIDAEEDAEIVIGTLAESREEVANHVQHGTIESILRYIKVKPGEF
                     YFIPAGTVHTISSGILAYETMQSSDITYRLYDFNRQDNQYNDRPLNIEKALDVIQYNA
                     PLPNILPESEIIENHKCTHIVSNDFFTLVKWEISGTLNYMKPREFCLVTVLEGEGQMI
                     VDGEIFKLTTGTNFILTSEDLDSVFEGDFTLMISYV"
     gene            complement(3790..4455)
                     /locus_tag="NBI57_001879"
     CDS             complement(3790..4455)
                     /locus_tag="NBI57_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCD1822814.1"
                     /translation="MNILVIGANGGVGSLLVQQLAKENVPFTAGVRQSDQLNALKSQG
                     MKAILVDVENDSIETLTETFKPFDKVIFSVGSGGNTGADKTIIVDLDGAVKSMIASKE
                     ANIKHYVMVSTYDSRRQAFDDSGDLKPYTIAKHYADDYLRRSGLNYTILHPGALTNAA
                     GSGKIEAAQYFDGKGEIPREDVATVLKEIVTSNHFNHQEFQIISGEQDIKDALTQFEI
                     EKD"
     gene            4607..4927
                     /gene="czrA"
                     /locus_tag="NBI57_001880"
     CDS             4607..4927
                     /gene="czrA"
                     /locus_tag="NBI57_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(II)-responsive metalloregulatory
                     transcriptional repressor CzrA"
                     /protein_id="HCD1822815.1"
                     /translation="MSEQYSEINTDTLERVTEIFKALGDYNRIRIMELLSVSEASVGH
                     ISHQLNLSQSNVSHQLKLLKSVHLVKAKRQGQSMIYSLDDIHVATMLKQAIHHANHPK
                     ESGL"
     gene            4929..5906
                     /gene="czrB"
                     /locus_tag="NBI57_001881"
     CDS             4929..5906
                     /gene="czrB"
                     /locus_tag="NBI57_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family zinc efflux transporter CzrB"
                     /protein_id="HCD1822816.1"
                     /translation="MSHSHHHDHMHSHVTTNNKKVLFISFLIIGLYMFIEIIGGLLAN
                     SLALLSDGIHMFSDTFSLGVALVAFIYAEKNATTTKTFGYKRFEVLAALFNGVTLFVI
                     SILIVFEAIKRFFVPSEVQSKEMLIISIIGLIVNIVVAFFMFKGGDTSHNLNMRGAFL
                     HVIGDLLGSVGAITAAILIWAFGWTIADPIASILVSVIILKSAWGITKSSINILMEGT
                     PSDVDIDEVITTIKKDSRIQSVHDCHVWTISNDMNALSCHVVVDHTLTMKECELLLEN
                     IEHDLLHLNIHHMTIQLETPNHKHDESIICSGTHSHSHNHHAHHHAHVH"
     gene            6172..>6878
                     /locus_tag="NBI57_001882"
     CDS             6172..>6878
                     /locus_tag="NBI57_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCD1822817.1"
                     /translation="MFTYFKSAFKNAKPQLLITLIYALIAFAVIAVVYLLANFQLAKY
                     AQTIAIYSQFGQKPPVDAYLKVIAVLLIAAVVSLFVLVQIFIGITNVMKRAMSHEKVK
                     FTDLFIAFKKGNYLKSVLIGLVSIAMIIVLSLLTSLLYKLFSPVSEMIMNSVQSSYAD
                     STHLIGIAITTQSIIIIVVLLIKVIITWLLLIPIFNFMTSFVESTNDKVKTHLANGFK
                     AMKNGQKTFFKFFIGIL"
BASE COUNT         2209 a         1244 c          941 g         2484 t
ORIGIN      
        1 agctcttatg gttgattacc cgtcattttt ctttatgcgg ttattcaaat atgcaattat
       61 gcaattttta atcctatcct tacttctttt caaaaactac catatatcat tagtcatgtt
      121 tgactttgac tatacgtgtg tattttaaaa actgctctga atatttctcc ttaatttcct
      181 ctaaactgtc atatgtaata cctacaatat gccaattagg attgaaataa tagaaagaat
      241 ctttttgtcg tgaccattta atcatattac cgtctttatt ataacgtggt aatgttactt
      301 catagccttc taacacatta atatacgttt ggaaaatgtc tgtatctata cgattaaaat
      361 tatcaataac taaataactt ttatcctttt taggcattaa ttgagtaata aacccttcac
      421 gatagtataa tgcccctgtt tcatttggta aatacttgcc ataaagcata tcttctgaaa
      481 aatcaggatg acctgttgtt ataacaggat ttgcattcgc tttttgcaat aagtcattag
      541 ccgcttttaa gccctcttct tttcgatcta caacaagtaa aataaatggc tttaacgctt
      601 cttctttttc actttcaagc atatctggtt ttttaaccat ttcaaatgga gatttcaatc
      661 cattattatc gctcatttca ataattgcat catactgtgc ttgtgacata cttgcaatag
      721 cctgttttgc attttcttga aggaaatata aatttttcaa tttaggatgc ttatttaatg
      781 tacttaatgt aatcggcgta atgtctttct cataagacac ttcaatcact gtactatttg
      841 ttctaccagg aattggtggt ttttcatgaa tatgctttga aacttctcca attccaacga
      901 cagattgatt tttcgttcga ttataaaaaa taatattgtc gccttcttct aactgagtat
      961 aaaaatgata accattacgt ttaataccgt tgtacgtgtg cgtataaatc gtatattggt
     1021 ttccaggttc aaattcttca gtttcagcta aaaagaaata acgtggtatc ttaatttcgc
     1081 ctttaccaag accacttatt aaatcaaact cttctgcagt gatttggttg aacaatgtct
     1141 ctttcatatt actcatacga aattccaaag cttcactacg ctttaaataa tcagctgtta
     1201 atggtttcaa ttgttcatta aaacgaaact gtacacgtat tttattttgt gcacctgttt
     1261 caacactaat aatttcacca catccaagta gtccagtatc cgtctgaact tgataaaaga
     1321 tgacttgatc tcctacttta gcctttttaa acgctctaaa tccttgagat gggttaaaat
     1381 gtgcgcctga ttcaaataaa gctgtttgtc ctactaacgg ttcattatga ttccaacggt
     1441 tatatccaca attcaaccaa aaataattcg tttctgctgt catcttaata ctccttaacc
     1501 tgaataaatt ttagaaacac tatgaattac attcttttag tgtttcttat gcagttggac
     1561 gcgtatgcga acaactgtat accctttgtt cactgcgatt ttaatcgcat ttcctataac
     1621 attgtagcgc ccaggacatt aatttacgtc ccagaccctt atcgttttca cttctaagta
     1681 agtcgaacta ttttgcttta caacaagtgc gactctaaat acagttggac acacatacga
     1741 gcaactgtat accttttaat cagttttcta tattttattt attatatctg tcttaatgat
     1801 aaaaattgtt acaaacagtt taacatattt agctacctat tttgcatata aaaaacatgc
     1861 ttgcatacac tatgcaataa aaattacaaa tttatatatg ataccactat gcttgcttat
     1921 ctctatagcg ccattgatac acatttttaa atatctatac tgccgttaga attttatcat
     1981 gtcttaattt tcattaaata ttaattactt cattttatat aaaccaacaa aaaccccctc
     2041 actactatgc aagtgagagg ttatgttgat gtgctttatt ttcatcaatg tcattaacac
     2101 gatcatattt cttctgttaa tgcactgtac tttattcaaa cgcgctatcg acaattaatt
     2161 tgaagctaca aaggtaccgc ataatggcag cacattaatt aacaatgcgc taaactttaa
     2221 tgaggcgctg atttgcttgt ctttaaaagt caacatagca ctgataattc ctaaaatagc
     2281 tactatacgt ggaattgtca tgctcggata ctgcggtata tgtaagaaag caccaagacc
     2341 tgctatcaca ccacatacta acgcaatata tagaaattgc ttcaaatcat tcactcctaa
     2401 attgttatta cactattaca catagctaat catcaatgtg aaatcgcctt caaagacact
     2461 atccaaatct tcagaagtca aaataaagtt tgtaccagta gtcagtttga aaatttcacc
     2521 atcgacaatc atttgtcctt caccttccaa cactgtaact aaacagaact ctctaggctt
     2581 catataattt aacgtgccag aaatttccca tttaaccaat gtaaagaaat cattcgatac
     2641 aatgtgtgta cacttatggt tttcaataat ttcactttca ggcaaaatat taggtaatgg
     2701 tgcattgtac tgaataacgt ctaaagcttt ttcaatattt aacggtctat cattatattg
     2761 attatcttga cgattgaaat cataaagtct atatgtaatg tctgacgatt gcatcgtttc
     2821 gtatgctaat attcctgaag aaatagtatg tactgttcct gctggaataa aatagaattc
     2881 tccaggtttt actttaatat atctaagtat cgactctatc gttccgtgtt gaacatgatt
     2941 cgcaacttct tctctagact ctgctaatgt ccctataact atttctgcat cttcttctgc
     3001 atctataata taccaacatt cagatttgcc atattgcccg ttttcatgct cataagcata
     3061 agaattatca gggtgcacat gaatagaaag tgattctctt gcatccacta ttttagttag
     3121 aagcggaaaa tctttgcttg ggaaatcacc aaacaattca cgatgttctg accaaatacg
     3181 gtctaatgtt tgaccttgat atggtccatt aataatctcg ctcgtaccat ttggatgtgc
     3241 tgacacacac caacattccc cagttgtatc attgtctaat tgatatccaa actcacttag
     3301 acgttgaccg ccccataatt ttgtttttaa aattggttgt aaaaataatg gcattgttgc
     3361 acctccattg tgattaagta agcaatagaa ctctgatgtt gttgttccat tatattttga
     3421 ttttgttctc atttacatcg tattataaac ttccacattt caaattaact attagtgatt
     3481 gtaccatatt tactaacatt gcagtactgc caattaaagc gcttcactta aatttacagt
     3541 actttaacat ttttaaaaat taatagcata gagattatct ctctcttaca tttgtacaca
     3601 tttcccttta aatttactcg cccattatac caattaataa acaactttaa tagttgtgcc
     3661 atacattgtt caaattcttt gtaaaatgca tagacaatac gtacttattc atacttataa
     3721 ttcatcattt tcaaaaaaat aacgagttac gaaaacgtaa cccgcttcaa atcatattta
     3781 ctatccttat taatcctttt caatttcaaa ttgagttaaa gcatctttaa tgtcctgctc
     3841 accactaata atttgaaact cttggtgatt aaaatgattg gatgtgacaa tttcttttaa
     3901 tactgtcgca acatcttctc taggaatttc acctttacca tcaaaatatt gtgcagcttc
     3961 tatctttcca gatcctgctg catttgtaag tgcccctgga tgtaaaattg tataattcaa
     4021 acctgaacgt cttaaatagt catcagcgta atgtttagct attgtatatg gctttaaatc
     4081 accgctatca tcaaaagcct gacgtctcga atcatatgtt gaaaccatga catagtgttt
     4141 aatattagcc tctttactcg caatcattga tttaacagca ccatctaaat cgacaataat
     4201 tgttttatct gcacccgtgt tccctccaga acctactgaa aagataactt tatcgaatgg
     4261 tttaaacgtc tcagttaaag tctctattga atcattttca acatcaacaa gaattgcttt
     4321 cataccttgt gattttaacg cattaagttg atctgattgc ctaacaccag cagtaaatgg
     4381 tacattttct tttgctaatt gttgcactag taacgaacct acaccgccat tagcacctat
     4441 aaccaaaata ttcatttaca acactctcct atttattatt ctctatgcca taccacttta
     4501 tgagatatgt aaaacttgtt acaactataa aaatcaattg acatactact gggaacgtat
     4561 taaattaata tatgaacaaa tattcatatg aaaggattgt catataatgt cagaacaata
     4621 ttcagaaata aatacagata cattagaacg cgtaactgaa attttcaagg cattaggcga
     4681 ttacaatcga atacgtatca tggaattgtt atcagtcagt gaagcaagtg ttggtcacat
     4741 ttcacatcaa ttgaatttat ctcaatcaaa tgtctcgcac caattaaaat tacttaaaag
     4801 tgtgcatctt gtgaaagcaa aacgacaagg ccaatcaatg atttattcat tagatgacat
     4861 ccacgtagca actatgttaa agcaagccat acatcacgcg aatcatccta aagaaagtgg
     4921 gttataatat gtctcattca catcatcatg accatatgca tagtcatgta actacaaata
     4981 ataagaaagt attgtttata tcgtttttaa taatcggtct atatatgttt atcgaaatca
     5041 tcggcggtct ccttgctaac agcttggcat tactatctga cggtatccat atgtttagcg
     5101 acacattctc attaggtgtt gcacttgtcg catttattta tgctgaaaag aatgccacaa
     5161 ctacaaaaac atttggttat aaacgtttcg aagtactcgc agcgttattt aacggtgtaa
     5221 cgctttttgt aataagtatt ttgattgttt ttgaagcgat taaacgtttc tttgttcctt
     5281 ctgaagttca atcaaaagaa atgttaatca ttagtattat cggtttaatt gtcaatatcg
     5341 ttgttgcatt ctttatgttt aaaggcggcg acacttcaca caatttaaat atgcgtggtg
     5401 cttttctaca tgttatcgga gacttattag gttcagttgg cgccattact gcagctattt
     5461 taatttgggc atttggatgg acaatcgccg atcctatcgc aagtatttta gtttccgtta
     5521 ttattttaaa aagtgcttgg ggtatcacaa aatcttcaat taacatttta atggaaggca
     5581 caccaagtga tgttgatata gatgaagtta taactactat taaaaaggat tcacgaatac
     5641 aaagtgtgca tgattgccat gtttggacaa tttcaaatga tatgaatgca ttgagttgtc
     5701 atgttgttgt agaccataca ttgacaatga aagaatgtga attattatta gaaaacattg
     5761 agcatgattt attacattta aatattcacc atatgactat tcaattagaa acgcctaatc
     5821 acaaacatga tgaatcgatt atatgttcag gaacacatag tcattcacat aaccatcatg
     5881 ctcatcatca cgcgcatgta cattaataat tttaacctac tgccattgca tcgattaaac
     5941 ttttcaatgg cagtaggttt tttatgtctt tatggcgact tgtttggtct ttgatgatgc
     6001 aatgtttatt aacaaatttt caactattat ttcttacatt agtcatattt ttgacaattt
     6061 actattataa ttctctaact ttagtcactt taattaattt ttattagata ttaatatgaa
     6121 aataacgtgt tttttgttat tgtatttata tgaagaaaag gaggcaccac tttgtttact
     6181 tactttaaat cagcatttaa aaatgctaaa ccacaattat taataacatt gatatatgca
     6241 cttatagcat ttgctgtaat tgcagttgta tatttacttg ctaatttcca gcttgctaag
     6301 tatgcacaaa ctattgcaat ttattcacaa tttgggcaaa aaccaccagt agatgcatat
     6361 ttaaaagtta tcgctgtatt acttatagca gctgtagttt cactgtttgt tttagttcaa
     6421 atttttatcg gaattacaaa tgttatgaaa cgagcaatga gtcatgaaaa agtaaaattt
     6481 accgatttat ttattgcatt taaaaaaggt aattatttaa agagcgttct gatcggtctt
     6541 gtatctatag caatgattat cgttctttca cttctgacat cattactata caaattattt
     6601 tctccagtat cagaaatgat tatgaattca gtacaatcat catatgcaga tagtacacac
     6661 ctgattggta ttgctattac aactcaaagc atcataatca ttgttgtttt attgattaaa
     6721 gtaattatta cttggttatt gttaatacct attttcaact ttatgactag tttcgttgaa
     6781 tcaactaacg ataaagtaaa aacacactta gctaatggat ttaaagcaat gaaaaatggt
     6841 caaaaaacat ttttcaaatt tttcattgga atactatt
//
LOCUS       DAHHSC010000126         6843 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.127, whole genome shotgun sequence.
ACCESSION   DAHHSC010000126 DAHHSC010000000
VERSION     DAHHSC010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 6843)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6843)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6843
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.127"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            complement(181..1167)
                     /locus_tag="NBI57_001883"
     CDS             complement(181..1167)
                     /locus_tag="NBI57_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HCD1822818.1"
                     /translation="MNDQWYKHLIGARTIKTGIAIFLTAVFCMALDLTPIYAILTAVV
                     TIEPTAKASLIKGYRRLPATVIGAGFAVLFTYLFGDQSPFTYALSATFTILFCTKLKL
                     QVGTNVAVLTSLAMIPGIHDAYIFNFLSRTLTAIIGLVTSGLINFMVFPPKYYGQVEE
                     KLSKTDALMYKLFYNRCQELILSRLQSDKSEKAYKNIFNLNNQVETLISYQRDELSYH
                     KKKECDWKLLNQLTKRAYTNRLFITHLSNIIYLPKNTRVNFSGDEKMALLKISSSIKD
                     IFYDGTFKREDDSVETLRSTIKALEISGENQIKSHILYEVLMIYRLLDSRYA"
     gene            1697..2455
                     /gene="map"
                     /locus_tag="NBI57_001884"
     CDS             1697..2455
                     /gene="map"
                     /locus_tag="NBI57_001884"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HCD1822819.1"
                     /translation="MIVKTEEELQALKEIGYICAKVRNTMQAATKPGITTKELDNIAK
                     ELFEEYGAISAPIHDENFPGQTCISVNEEVAHGIPSKRVIREGDLVNIDVSALKNGYY
                     ADTGISFVVGESDDPMKQKVCDVATMAFENAIAKVKPGTKLSNIGKAVHNTARQNDLK
                     VIKNLTGHGVGLSLHEAPAHVLNYFDPKDKTLLTEGMVLAIEPFISSNASFVTEGKNE
                     WAFETSDKSFVAQIEHTVIVTKDGPILTTKIEEE"
     gene            2672..3058
                     /locus_tag="NBI57_001885"
     CDS             2672..3058
                     /locus_tag="NBI57_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1822820.1"
                     /translation="MNYVERYIEQFLRATVRNNIKHYLLMLDEKMKNLDDYMRYLITK
                     KEQLSKLIDSLMLTLENKYIDIAEAFQIQCAREINNQEIENIKSELNKVEAYYAQIET
                     QIQQTSTEKIATEKTSYLINYMNAVA"
     gene            3073..3774
                     /locus_tag="NBI57_001886"
     CDS             3073..3774
                     /locus_tag="NBI57_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein"
                     /protein_id="HCD1822821.1"
                     /translation="MTHKYISTQMLIIFTALMIIANFYYIFFEKIGFLLVLLLGCVLV
                     YVGYLYFHKIRGLLAFWIGALLIAFTLLSNKYTIIILFVFLLLLIVRYLIHKFKPKKV
                     VATDEVMTSPSFIKQKWFGEQRTPVYVYKWEDVQIQHGIGDLHIDLTKAANIKENNTI
                     VVRHILGKVQVILPVNYNINLHVAAFYGSTYVNEKSYKVENNNIHIEEMMKPDNYTVN
                     IYVSTFIGDVEVIYR"
     gene            3771..4814
                     /locus_tag="NBI57_001887"
     CDS             3771..4814
                     /locus_tag="NBI57_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HCD1822822.1"
                     /translation="MNHYIRTIGSMLILVYSMLAAFLFIDKVFVNIIYFQGMFYTQIF
                     GIPVFLFLNLIIILLCIIVGSVLAYKINQQNDWIKTQIERSMEGETVGINDQNIEIYS
                     ETLDLYHTLVPLNQELHKLRLKTQNLTNENYNINDVKVKKIIEDERQRLARELHDSVS
                     QQLFAASMMLSAIKETKLEPPLDQQIPILEKMVQDSQLEMRALLLHLRPLGLKDKSLG
                     EGIKDLVIDLQKKVPMKVVHEIQDFKVPKGIEDHLFRITQEAISNTLRHSNGTKVTVE
                     LFNKDDYLLLRIQDNGKGFNVDEKLEQSYGLKNMRERALEIGATFHIVSLPDSGTRIE
                     VKAPLNKEDSYDD"
     gene            4804..5433
                     /gene="vraR"
                     /locus_tag="NBI57_001888"
     CDS             4804..5433
                     /gene="vraR"
                     /locus_tag="NBI57_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator VraR"
                     /protein_id="HCD1822823.1"
                     /translation="MTIKVLFVDDHEMVRIGISSYLSTQSDIEVVGEGASGKEAIAKA
                     HELKPDLILMDLLMEDMDGVEATTQIKKDLPQIKVLMLTSFIEDKEVYRALDAGVDSY
                     ILKTTSAKDIADAVRKTSRGESVFEPEVLVKMRNRMKKRAELYEMLTEREMEILLLIA
                     KGYSNQEIASASHITIKTVKTHVSNILSKLEVQDRTQAVIYAFQHNLIQ"
     gene            complement(5546..6763)
                     /locus_tag="NBI57_001889"
     CDS             complement(5546..6763)
                     /locus_tag="NBI57_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HCD1822824.1"
                     /translation="MSKKDHSSSKYLNSVKEAQEESKKKNKSNPKIDVDRTYIEPQQF
                     QSKKPKKDDQVFFLSRLNKPAKYKKDSNFLSYLIYRIGKDDASGLAAQMTYHFVLAMF
                     PMLLFLLTLLPFFNIKQSQITNMLSNAPAETSTLIKSVIGDITQNSSGGLLSIGLILA
                     IWSASNGMTAIMNSFNVAYDVEDSRNGIVLKLLSVVFTVVMGVVFVVALALPTLGSVI
                     SHFLFGPLGFDEQVKWIFNLIRIVLPIIIIFIIFIVLYSVAPNVKTKLKSVLPGAVFT
                     SIIWLAGSFGFGWYISNFGNYSKTYGSIAGIIILLLWLYITSFIIIVGAEINAIIHQR
                     SVIKGKTPEEAALEHDDNNKNHYNENTRYEYDEDSNATHQRTYHVDEHPSDTYPEDDK
                     NIKDKIVDKLKKD"
BASE COUNT         2437 a         1018 c         1200 g         2188 t
ORIGIN      
        1 agagaaattg gattcccaat ttctacagac aatgcaagtt ggggtgtggg tcccaacaca
       61 gagaatttcg aaaagaaatt ctacaggcaa tgcgagttgg ggtgggacga cgaaataaat
      121 tttacgaaaa tatcatttct gtcccactcc catggtgcca attagcataa ggtacttaaa
      181 ttaagcatat ctgctgtcta gcagtcgata aatcattaga acttcgtata gtatatgact
      241 tttaatttga ttttcaccac taatttcaag tgcttttata gtcgaacgta aagtttctac
      301 agaatcatct tctctcttaa aagtaccatc ataaaatata tctttgatgc tactactaat
      361 ttttagcaat gccatttttt cgtcacctga aaagttaaca cgagtatttt taggcaagta
      421 aatgatattt gataaatgag tgataaacaa acgattcgta tatgcacgtt tagttaattg
      481 attgagtaat ttccaatcac attctttttt cttatgatag cttaattcat cacgttgata
      541 acttattaac gtttcaactt gattgtttaa attgaaaata tttttatatg ctttttcgct
      601 tttatcagat tgcagtcttg ataagataag ttcttggcag cgattgtaaa ataatttata
      661 catcaaggca tctgtcttac ttaatttttc ttcgacctga ccataatact taggtggaaa
      721 caccatgaag ttaattaaac ctgatgtcac gagtccaata attgctgtca atgttcgaga
      781 caaaaagttg aatatgtagg catcatgaat acctggaatc atagctaatg atgttagtac
      841 agcgacattc gtaccaactt gcaatttgag ttttgtacag aataaaatcg tgaacgttgc
      901 actcaatgca tatgtaaaag gtgattgatc gccgaataaa tatgtaaata atactgcaaa
      961 gcctgcacca attaccgtag caggtaatct acgataacct ttaataagtg atgccttggc
     1021 agttggttca attgtgacta cagctgttaa aatggcatag atgggtgtta aatctagtgc
     1081 catacaaaag acagctgtta aaaaaatggc aataccagtt ttaattgttc tggcaccaat
     1141 taaatgttta taccattgat cgttcatttt ttaacctcta atcatcgtaa aatcttagcg
     1201 agcgctttat aataatagta tcgtacattg gaaaagttca tgtatgtaaa atatttgaaa
     1261 taatcatact taagcattac tttgattttc atatacatta atcaaagttc tgggctccac
     1321 aatgttatat tgttagtagt tgactgaaat aaaatgcact tgtaacaagc atttttcaat
     1381 tatagtccgg ggccccaaca cagaagctga cgaaaagtca gcttacaata atgtgcaagt
     1441 tggggtgtgg gccccaacac agaagctgac gaaaagtcag cttacgataa tgtgcaggtt
     1501 ggcggggccc caacatagag aaattggatc tacaatttct acaggcaatg caagttgggg
     1561 tacaacgata aagaaatatt ttttctttat cacactatgt ctcactcact ttccaaaata
     1621 ctaaagtaac atctttagta tatcaaagaa ttttgctata ataagttata attatataaa
     1681 aaaggaacgg gataaaatga ttgtaaaaac agaagaagaa ttacaagcgt taaaagaaat
     1741 tggatacata tgcgctaaag tgcgcaatac aatgcaagct gcaaccaaac caggtatcac
     1801 tacgaaagag cttgataata ttgcgaaaga gttatttgaa gaatacggtg ctatttctgc
     1861 gccaattcat gatgaaaatt ttcctggtca aacgtgtatt agtgtcaatg aagaggtggc
     1921 acatgggatt ccaagtaagc gtgtcattcg tgaaggagat ttagtaaata ttgatgtatc
     1981 ggctttgaag aatggctatt atgcagatac aggcatttca tttgtcgttg gagaatcaga
     2041 tgatccaatg aaacaaaaag tatgtgacgt agcaacgatg gcatttgaga atgcaattgc
     2101 aaaagtaaaa ccgggtacta agttaagtaa cattggtaaa gcggtgcata atacagctag
     2161 acaaaatgat ttgaaagtca ttaaaaactt aacaggtcat ggtgttggtt tatcattaca
     2221 tgaagcacca gcacatgtac ttaattactt tgatccaaaa gacaaaacat tattaactga
     2281 aggtatggta ttagctattg aaccgtttat ctcatcaaat gcatcatttg ttacagaagg
     2341 taaaaatgaa tgggcttttg aaacgagcga taaaagtttt gttgctcaaa ttgagcatac
     2401 ggttatcgtg actaaggatg gtccgatttt aacgacaaaa attgaagaag aatagttcaa
     2461 catatactaa gactaaagta tgaacatcat ttagttccgg agcctattca tattggtttc
     2521 ggaactgttt tataataatt aagaacacaa tcaatgcgtc atttcaaaaa tatgttgtaa
     2581 taaagtagtt tttaagcaaa catatcatcg acatcaacga agatacatag cgcatttggt
     2641 attttaaaac ttattataaa aggtgatagt tatgaactat gttgaacgtt atattgaaca
     2701 gtttttgaga gcaacagtaa gaaataatat caagcactac cttttaatgc tagatgaaaa
     2761 aatgaaaaat ttagatgatt atatgcgtta tttaattact aaaaaagaac aacttagcaa
     2821 gttaattgac agtctaatgc taacattaga aaataaatat attgatattg ctgaagcatt
     2881 tcaaattcaa tgtgcaagag aaatcaataa tcaagaaatt gaaaatatta aatcagagtt
     2941 gaataaagtt gaagcatatt atgcacaaat tgaaactcaa attcaacaaa cttcaactga
     3001 aaaaatagca acagaaaaaa catcgtatct aataaattat atgaacgctg tggcatagaa
     3061 aggcggcgaa acatgacaca caaatatata tcaacgcaaa tgttgatcat ttttactgca
     3121 ttaatgatta ttgccaattt ttactacata ttttttgaaa aaattggctt tttactcgtt
     3181 ctattattgg gatgtgtatt agtttatgta ggatatcttt attttcataa aatacgtggc
     3241 cttttagcgt tttggatagg cgcgctatta attgcattca cattattgtc taataagtat
     3301 acaatcatca tcttgttcgt ctttttatta ctacttattg tgcgttattt aatacataaa
     3361 tttaaaccaa aaaaagtagt tgcgacggat gaggttatga cttcaccatc ttttattaaa
     3421 caaaagtggt ttggtgagca acgtacacca gtttatgtat ataagtggga agatgtccaa
     3481 attcaacatg gaattggcga cctacatatt gacttaacaa aagctgcaaa tattaaggaa
     3541 aataatacca ttgttgttag acacatttta ggtaaagtgc aggttatatt gccggttaat
     3601 tacaatatta atttacatgt agctgctttt tatggaagta cttacgtgaa tgaaaaatca
     3661 tataaagttg aaaataacaa tattcatatt gaagaaatga tgaaaccgga taactataca
     3721 gttaatatct acgtatcaac gtttatcgga gacgtagagg tgatttatcg atgaaccact
     3781 acattagaac aattggttca atgctcatct tagtatatag catgctagct gcatttctat
     3841 tcatcgataa agtttttgta aatatcatct attttcaagg tatgttttat acacaaatat
     3901 tcggaatacc agtcttttta tttttaaatc tcatcatcat attattgtgt attattgttg
     3961 gttcggtact cgcatacaaa atcaatcagc aaaatgattg gattaagacg caaattgagc
     4021 gttcaatgga aggcgaaaca gttggcatta atgatcaaaa tatagaaata tatagtgaaa
     4081 cgttagattt ataccataca ctcgtacctt taaatcaaga attgcataag ttgcgactta
     4141 aaactcaaaa cttaaccaat gaaaattata atattaatga tgtgaaagtt aaaaagatta
     4201 ttgaagatga acgtcaaaga ctagctcgag aacttcacga ttctgttagt cagcaacttt
     4261 ttgcggcaag tatgatgctt tctgctatca aagaaacgaa gttagaacca ccattagacc
     4321 aacaaattcc gattttagag aaaatggttc aagattcgca gttagaaatg cgtgctttgc
     4381 tgttacattt aagaccgctt ggtttaaaag acaaatcttt aggtgagggt attaaagatt
     4441 tagttattga tttacaaaaa aaagtgccaa tgaaagttgt gcatgaaata caagatttta
     4501 aagtgcctaa aggtattgaa gatcatttgt tcagaattac acaggaagca atttcgaata
     4561 cattgcgtca ttcaaacggt acaaaagtga cagtagaatt gtttaataaa gacgattatt
     4621 tattgttgag aattcaagat aatggtaaag gttttaatgt tgatgaaaaa ttagaacaaa
     4681 gttatggact taaaaatatg cgtgaaagag ctttggaaat tggtgcaacg ttccatattg
     4741 tatcattgcc agattcaggt acacgtatcg aggtgaaagc acctttaaat aaggaggatt
     4801 cgtatgacga ttaaagtatt gtttgtggat gatcatgaaa tggtacgtat aggaatttca
     4861 agttatctat caacgcaaag tgatattgaa gtagttggtg aaggcgcttc tggtaaagaa
     4921 gcaattgcca aagcccatga gttgaagcca gatttaattt taatggattt acttatggaa
     4981 gacatggatg gtgtagaagc gacgactcag attaaaaaag atttaccgca aattaaagta
     5041 ttaatgttaa ctagttttat tgaagataaa gaggtatatc gtgcattaga tgcaggtgtc
     5101 gatagttaca ttttaaaaac aacaagtgca aaagatatcg ccgatgcagt tcgtaaaact
     5161 tctagaggag aatctgtttt tgaaccggaa gttttagtga aaatgcgtaa ccgtatgaaa
     5221 aagcgcgcag agttatatga aatgcttaca gaacgagaaa tggaaatatt attattgatt
     5281 gcgaaaggtt actcaaatca agaaattgct agtgcatcgc atattactat taaaacggtt
     5341 aagacacatg tgagtaacat tttaagtaag ttagaagtgc aagatagaac acaagctgtc
     5401 atctatgcat tccaacataa tttaattcaa tagttcgtat cgaattaaga aaagttactt
     5461 acgccaatca caatataaca tcaaatagac accaaaaaac agaggtcttc gcaatcatta
     5521 gcgaaaacct ctgttttgtt ggtgtttagt cttttttaag cttatcaacg attttgtctt
     5581 ttatattttt atcatcttct ggataggtat cactaggatg ttcatcaaca tgatacgtac
     5641 gttgatgtgt tgcattacta tcttcatcat attcgtatct agtgttctca ttataatgat
     5701 ttttattatt gtcatcatgt tctaatgctg cttcttcagg tgttttacct ttaattacac
     5761 tacgctgatg aatgattgca ttgatttcag caccgacaat tataataaaa cttgtgatat
     5821 ataaccatag taataaaatg atgatacccg cgatactgcc atatgtttta gaatagttac
     5881 caaaatttga aatataccaa ccaaaaccaa atgaaccagc taaccaaata attgaagtaa
     5941 atactgcacc tggtaatact gacttaagct tcgttttaac gttaggtgca accgaatata
     6001 acacgataaa tatgataaat ataataatga ttggtaacac aattctaata aggttaaaaa
     6061 tccatttcac ttgttcgtca aatccaagtg gaccgaatag gaaatgacta attacagaac
     6121 caagcgttgg taatgctaga gcaactacaa acacaacgcc cataactaca gtgaagacaa
     6181 cacttagtag ttttaaaacg attccattac ggctatcttc tacatcgtaa gcaacattga
     6241 aagaattcat aattgcagtc attccatttg aagctgacca aattgctaaa atcaaaccga
     6301 tagataataa gccaccactg gagttttgag ttatatcacc aattacactc ttaattagag
     6361 tagatgtttc agcgggtgca ttgcttaaca tattagtaat ttgactctgc ttaatattga
     6421 aaaatggtaa taatgttaat aggaaaagca acatagggaa catagcaagt acgaaatggt
     6481 aagtcatttg cgctgctagt cctgaggcat catcttttcc tatgcgatag atgagatatg
     6541 ataagaaatt agagtctttc ttatattttg caggtttatt taatcttgat aagaagaaaa
     6601 cctgatcatc ttttttaggt ttcttagatt ggaattgttg aggttcaata tatgtacgat
     6661 caacatcaat tttgggatta cttttatttt tcttttttga ctcctcttgc gcttccttaa
     6721 cagaattaag gtattttgaa gaagagtgat cctttttcga catgtgcact ccttaacctt
     6781 tctctatgta atgttttata aagtaactat tagtatttat ccaattataa aaaaactaaa
     6841 caa
//
LOCUS       DAHHSC010000127         6824 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.128, whole genome shotgun sequence.
ACCESSION   DAHHSC010000127 DAHHSC010000000
VERSION     DAHHSC010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 6824)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6824)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6824
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.128"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            43..1071
                     /gene="sspA"
                     /locus_tag="NBI57_001890"
     CDS             43..1071
                     /gene="sspA"
                     /locus_tag="NBI57_001890"
                     /EC_number="3.4.21.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Glu-specific serine endopeptidase SspA"
                     /protein_id="HCD1822825.1"
                     /translation="MKGKFLKVSSLFVATLTTATLVSSPAANALSSKAMDNHPQQTQS
                     SKQQTPKIKKGGNLKPLEQREHANVILPNNDRHQITDTTNGHYAPVTYIQVEAPTGTF
                     IASGVVVGKDTLLTNKHVVDATHGDPHALKAFPSAINQDNYPNGGFTAEQITKYSGEG
                     DLAIVKFSPNEQNKHIGEVVKPATMSNNAETQVNQNITVTGYPGDKPVATMWESKGKI
                     TYLKGEAMQYDLSTTGGNSGSPVFNEKNEVIGIHWGGVPNEFNGAVFINENVRNFLKQ
                     NIEDIHFANDDQPNNPDNPDNPNNPDNPNNPDNPNNPDEPNNPDNPNNPDNPDNGDNN
                     NSDNPDAA"
     gene            1153..2334
                     /gene="sspB"
                     /locus_tag="NBI57_001891"
     CDS             1153..2334
                     /gene="sspB"
                     /locus_tag="NBI57_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine protease staphopain B"
                     /protein_id="HCD1822826.1"
                     /translation="MNSSYKSRVFNIISIIMVSMLILSLGAFANNNKAKADSHSKQLE
                     INVKSDKVPQKVKDLAQQQFAGYAKALDKQSNAKTGKYELGEAFKIYKFNGEEDNSYY
                     YPVIKDGKIVYTLTLSPKNKDDLNKSKEDMNYSVKISNFIAKDLDQIKDKNSNITVLT
                     DEKGFYFEEDGKVRLVKATPLPGNVKEKESAKTVSAKLKQELKNTVTPTKVEENEAIQ
                     EDQVQYENTLKNFKIREQQFDNSWCAGFSMAALLNATKNTDTYNAHDIMRTLYPEVSE
                     QDLPNCSTFPNQMIEYGKSQGRDIHYQEGVPSYEQVDQLTKDNVGIMILAQSVSQNPN
                     DPHLGHALAVVGNAKINDQEKLIYWNPWDTELSIQDADSSLLHLSFNRDYNWYGSMIG
                     Y"
     gene            2372..2701
                     /gene="sspC"
                     /locus_tag="NBI57_001892"
     CDS             2372..2701
                     /gene="sspC"
                     /locus_tag="NBI57_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphostatin B"
                     /protein_id="HCD1822827.1"
                     /translation="MYQLQFINLVYDTTKLTHLEQTNINLFIGNWSNHQLQKSICIRH
                     GDDTSHNQYHILFIDTAHQRIKFSSIDNEEITYILDYDDTQHILMQTSSKQGIGTSRP
                     IVYERLV"
     gene            complement(2939..3760)
                     /gene="menB"
                     /locus_tag="NBI57_001893"
     CDS             complement(2939..3760)
                     /gene="menB"
                     /locus_tag="NBI57_001893"
                     /EC_number="4.1.3.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA synthase"
                     /protein_id="HCD1822828.1"
                     /translation="MTNRQWETLREYDEIKYEFYEGIAKVTINRPEVRNAFTPKTVAE
                     MIDAFSRARDDQNVSVIVLTGEGDLAFCSGGDQKKRGHGGYVGEDQIPRLNVLDLQRL
                     IRIIPKPVIAMVKGYAVGGGNVLNVVCDLTIAADNAIFGQTGPKVGSFDAGYGSGYLA
                     RIVGHKKAREIWYLCRQYNAQEALDMGLVNTVVPLDKVEDETVQWCKEIMKHSPTALR
                     FLKAAMNADTDGLAGLQQMAGDATLLYYTTDEAKEGRDAFKEKRDPDFDQFPKFP"
     gene            complement(3753..4556)
                     /gene="menH"
                     /locus_tag="NBI57_001894"
     CDS             complement(3753..4556)
                     /gene="menH"
                     /locus_tag="NBI57_001894"
                     /EC_number="4.2.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-6-hydroxy-2,
                     4-cyclohexadiene-1-carboxylate synthase"
                     /protein_id="HCD1822829.1"
                     /translation="MTHYKYYEANVETNQVLVLLHGFLSDSRTYYNHIDKYTDICHVI
                     TIDLPGHGEDQSSMDETWNFDYITTLLDRILDKYKDKSITLFGYSMGGRVALYYAING
                     HIPISNLILESTSPGIKEEANQLERRLVDDARAKVLDIAGIELFVNDWEKLPLFQSQL
                     ELPVEIQHQIRLQRLSQSPQKMAKALRDYGTGQMPNLWPRLKEIKVPTLILAGEYDEK
                     FVQIAKKMANLIPNSKCKLISATGHTIHVEDSDEFDTMILGFLKEEQND"
     gene            complement(4543..6216)
                     /gene="menD"
                     /locus_tag="NBI57_001895"
     CDS             complement(4543..6216)
                     /gene="menD"
                     /locus_tag="NBI57_001895"
                     /EC_number="2.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-5-enolpyruvyl-6-hydroxy-3-
                     cyclohexene-1-carboxylic-acid synthase"
                     /protein_id="HCD1822830.1"
                     /translation="MGNHKAALTKQVFTFASELYAYGVREVVISPGSRSTPLALAFEA
                     HPNIKTWIHPDERSAAFFAVGLIKGSERPVAILCTSGTAAANYTPAIAESQISRIPLI
                     VLTSDRPHELRSVGAPQAINQVNMFNNYVSYEFDMPIADDSKETINAIYYQMQIASQY
                     LYGPHKGPIHFNLPFRDPLTPDLNATELLTSEMKILPHYQKSIDASALRHILNKKKGL
                     IIVGDMQHQEVDQILTYSTIYDLPILADPLSHLRKFDHPNVICTYDLLFRSGLDLNVD
                     FVIRVGKPVISKKLNQWLKKTDAFQILVQNNDKIDVFPIAPDISYEISANDFFRSLME
                     DTTINRVSWLEKWQCLEKKGRKEIKCYLEQATDESAFVGELIKKTSEKDALFISNSMP
                     IRDVDNLLLNKNIDVYANRGANGIDGIVSTALGMAVHKRITLLIGDLSFYHDMNGLLM
                     SKLNNIQMNIVLLNNDGGGIFSYLPQKESATDYFERLFGTPTGLDFEYTAKLYQFDFK
                     RFNSVSEFKNATLLSETSTIYELITNREDNFKQHQILYQKLSEMIHDTL"
     gene            complement(6203..>6824)
                     /locus_tag="NBI57_001896"
     CDS             complement(6203..>6824)
                     /locus_tag="NBI57_001896"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000190617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="isochorismate synthase"
                     /protein_id="HCD1822831.1"
                     /translation="IFFSQTPEQLIKVNNKILSTKAVAGTIKRSQDEDEDTKNVEAFL
                     KDNKNLIEHRFVVDSILHDIKPYITELHYDKTPKILKNDHLYHLYTEIKAPLKDDSYI
                     SLIDHLHPTPALGGYPKEFAMDFIEQKEFGTRGLYGAPVGYIDIYDDCEFIVAIRSML
                     IKKAQATLFAGCGIVKDSDPDSELAETNLKFTPMMNALGVDMNGKS"
BASE COUNT         2343 a         1272 c         1009 g         2200 t
ORIGIN      
        1 tgttatattt aacttgtaaa taaatttttt ggaggttttt agatgaaagg taaattttta
       61 aaagttagtt ctttattcgt tgcaactttg acaacagcga cacttgtgag ttctccagca
      121 gcaaacgcgt tatcatcaaa agctatggac aatcatccac aacaaacgca gtcaagcaaa
      181 cagcaaacac ctaagattaa aaaaggcggt aaccttaaac cattagaaca acgtgaacac
      241 gcaaatgtta tattaccgaa taacgatcgt caccaaatca cagatacaac gaatggtcat
      301 tatgcacccg taacttatat tcaagttgaa gcacctactg gtacatttat tgcttccggt
      361 gtagttgtag gtaaagatac tcttttaaca aataaacacg tcgtagatgc tacgcacggt
      421 gatcctcatg ctttaaaagc attcccttct gcaattaacc aagacaatta tccaaatggt
      481 ggtttcactg ctgaacaaat cactaaatat tcaggcgaag gtgatttagc aatagttaaa
      541 ttctccccta atgagcaaaa caaacatatt ggtgaagtag ttaaaccagc aacaatgagt
      601 aataatgctg aaacacaagt taaccaaaat attactgtaa caggatatcc tggtgataaa
      661 cctgtagcaa caatgtggga aagtaaagga aaaatcactt acctcaaagg cgaagctatg
      721 caatatgatt taagtacaac tggtggtaac tcaggttcac ctgtatttaa tgaaaaaaat
      781 gaagtgatcg gaattcattg gggcggtgta ccaaatgaat ttaatggtgc ggtatttatt
      841 aatgaaaatg tacgcaactt cttaaaacaa aatattgaag atatccattt tgccaacgat
      901 gaccaaccta ataacccaga taatcctgat aaccctaaca atcctgataa ccctaacaat
      961 cctgataacc ctaacaaccc agatgaacca aataaccctg acaaccctaa caaccctgat
     1021 aatccagaca atggcgataa caataattca gacaaccctg acgctgcata aacttcatct
     1081 taatttagat ttagtaaata gaagcaaagt cttagcaacg caaaatattt tgatttaaat
     1141 ggagggtatt atatgaatag ttcatataaa tctagagtat tcaatattat aagcatcata
     1201 atggtttcaa tgcttatttt atcactaggc gcatttgcta ataacaataa agccaaagcc
     1261 gattcacact ctaaacagct agaaattaat gttaagagtg acaaagtacc tcaaaaagta
     1321 aaagatctag cacaacaaca atttgctggt tatgctaaag cattagataa acaaagtaat
     1381 gcaaaaactg gtaaatatga acttggcgaa gcttttaaaa tttataaatt taatggtgaa
     1441 gaagataata gttactatta tccagttata aaagacggta aaattgttta tactttaaca
     1501 cttagcccta aaaataaaga tgatttaaac aaatcaaaag aagacatgaa ttacagtgtt
     1561 aaaatttcaa acttcatcgc taaagattta gaccaaatta aagataaaaa ttcaaatatc
     1621 actgttctta ctgatgaaaa agggttttat tttgaagaag atggcaaagt tagattagta
     1681 aaagctacgc cactacctgg taatgtaaaa gaaaaagaaa gtgctaaaac agtttcagca
     1741 aaattgaaac aagagttaaa aaatacagta acacctacta aagttgaaga aaacgaagcg
     1801 atacaagaag atcaagttca atatgaaaat acattaaaaa acttcaaaat tagagaacaa
     1861 caattcgata actcatggtg tgcaggattt agtatggcag cattattaaa tgcaactaaa
     1921 aatacagaca cttataatgc acatgatatt atgcgtacat tataccctga agtaagtgag
     1981 caagaccttc ctaattgctc aacattccct aatcaaatga ttgaatacgg taaatcacaa
     2041 ggcagagata ttcattatca agaaggcgta ccatcatatg aacaagttga tcaacttaca
     2101 aaagataatg taggaattat gattcttgca caaagtgtat ctcaaaaccc taatgatcca
     2161 catttaggac atgccctagc agttgttggt aatgctaaaa ttaatgacca agaaaaactt
     2221 atttactgga atccttggga tacagaatta tcaatccaag atgcagattc aagcctatta
     2281 catttatcat tcaatcgtga ttataactgg tatggttcaa tgataggtta ctaaaaagta
     2341 atatagatat tgattaaagg caggtaaaac tatgtatcaa ctacaattta taaatttagt
     2401 ttacgacaca accaaactca cacatctaga acaaaccaat atcaatttat tcattggtaa
     2461 ttggagtaat catcaattac aaaaatcaat ttgtatacgt catggcgatg atacaagtca
     2521 caatcaatat catattcttt ttatagatac ggcacatcaa cgcattaaat tttcatctat
     2581 tgataatgaa gaaatcactt atattcttga ttatgatgat acacagcata tcctcatgca
     2641 aacgtcatcc aaacaaggta ttggcacttc gcgcccaatc gtttatgagc gcttagtata
     2701 actaatttaa atgatttcac ttcataaagc gggttggcga gaattcaatt tctcaccagc
     2761 tcgttttttc attgtaataa taatctttaa catttattct ttctctatta atttactcaa
     2821 accatcttat ctttatgata attaattaaa attctctttt aaattcttat aaaataaaaa
     2881 agccacctat cgtcgctaat aaacgacgca agtgacttaa tatcatattc aaaataactt
     2941 atgggaattt agggaattga tcgaagtcag gatcacgttt ttctttaaac gcatcacggc
     3001 cttctttcgc ttcatcagtt gtgtaataaa gcaatgttgc atccccagcc atttgttgta
     3061 aaccagctaa accatctgtg tcagcattca tagctgcttt aaggaatcgt aaagctgttg
     3121 gtgagtgttt cataatctct ttacaccatt gcacagtttc atcttcaact ttatctaaag
     3181 gtaccactgt atttactagg cccatatcta aagcttcttg tgcattgtat tgacgacata
     3241 agtaccagat ttcacgtgct ttcttatgtc caacgatacg tgctaaatat cctgaaccat
     3301 aacctgcatc aaatgaacct actttaggac cagtttgtcc aaaaatagca ttatcagcag
     3361 caatcgttaa gtcacaaaca acatttagta cattaccgcc acctacagca taacctttta
     3421 ccatcgcgat aaccggtttt ggaataatac gaattaaacg ctgtaaatct aatacattta
     3481 agcgagggat ttggtcttca cctacataac caccatgtcc acgtttcttc tggtcaccac
     3541 cagaacagaa tgctaaatca ccttcaccag ttaatacgat aactgaaacg ttttgatcat
     3601 cacgtgcacg tgaaaatgcg tcaatcattt cagcaactgt tttaggtgta aacgcattgc
     3661 gtacttcagg gcgatttatt gttaccttag caatcccttc gtaaaattca tatttgattt
     3721 catcatattc tctaagtgtt tcccattgtc tgttagtcat tttgctcctc ctttaaaaat
     3781 cctaatatca ttgtatcaaa ttcatcacta tcttccacat gaattgtatg acctgtagca
     3841 gaaattaatt tacatttact attaggaatt aaatttgcca tttttttcgc aatctgtaca
     3901 aatttttcat catattctcc agctaatatt aatgttggta ctttaatttc tttcaggcgc
     3961 ggccataagt ttggcatttg acctgtacca taatctctta atgctttggc cattttctgt
     4021 ggcgattgag acaatcgttg tagtcttatt tgatgttgta tttcaactgg taattctagt
     4081 tgcgattgaa ataatggcaa cttttcccaa tcattaacaa ataattcaat acctgctatg
     4141 tctaatactt tagcacgtgc atcatcaaca agacggcgtt ccaattgatt tgcttcttct
     4201 ttaatacctg gtgacgtact ttctaatatc aaattagata tagggatgtg accattaatt
     4261 gcataatata atgcaacacg cccacccatt gaatatccaa acaatgttat tgatttatct
     4321 ttatatttat ctaaaattcg gtctaacaac gtcgtaatat aatcaaaatt ccacgtttca
     4381 tccattgaag actgatcttc gccatggcct ggtaagtcta tagtgatgac atgacagata
     4441 tcagtatatt tatcgatgtg attataataa gtacgactgt cgctaagaaa accatgcagt
     4501 aatactaaaa cttgattggt ctcaacgttt gcttcatagt atttataatg tgtcatgaat
     4561 catttcactc aatttctgat ataaaatttg atgctgttta aagttatctt cgcgattcgt
     4621 tatcaattca taaatcgtcg aagtttcaga taacaatgtg gcatttttaa attctgaaac
     4681 actgttaaaa cgtttaaaat cgaattgata taacttagct gtatactcga aatccaatcc
     4741 cgtcggtgtg ccaaacaacc gttcaaaata gtcagttgca ctttcttttt gtggtaaata
     4801 tgaaaaaata ccgccaccat cgttgttcaa taatacaata ttcatctgaa tattatttaa
     4861 ttttgacatt aatagtccat tcatatcatg ataaaatgac aaatcaccta tcaataatgt
     4921 tattcgttta tgcacagcca tacccagtgc agttgaaacg ataccatcaa taccattcgc
     4981 accacgattc gcatagacat ctatattttt attcaataac aagttatcta catctctgat
     5041 aggcatacta ttactaataa ataatgcatc tttttcagat gttttcttaa tcaattcacc
     5101 aacgaatgca ctctcatctg tagcttgttc caaataacat ttaatttctt tacgcccttt
     5161 tttctctaag cattgccatt tttctaacca acttacgcga ttgatggtcg tgtcttccat
     5221 taatgatcta aagaaatcat ttgcagaaat ctcatatgaa atatctggcg ctataggaaa
     5281 gacatcaatc ttatcattgt tttgcactaa tatttgaaat gcatcagttt tctttaacca
     5341 ttgattcaac tttttagaaa tcactggttt cccaacacga attacgaaat ccacatttaa
     5401 gtctaagccg cttctaaaca gcaaatcata tgtacagata acattcggat gatcaaattt
     5461 tcttaaatga cttaaaggat cagctaaaat aggcaaatca tatatcgttg aatacgttag
     5521 tatttgatca acttcttggt gctgcatatc ccctacaata attaaacctt ttttcttatt
     5581 taaaatgtgt cttaatgccg atgcatctat acttttttga tagtgcggta aaatcttcat
     5641 ctcagaagtt aacaattctg ttgcattcaa atcaggtgtt aacggatctc taaatggcaa
     5701 gttaaaatga attggccctt tatgtggtcc atataaatat tgactagcaa tttgcatttg
     5761 ataataaatt gcattaatgg tctctttact atcatccgca ataggcatat cgaactcata
     5821 acttacataa ttattaaaca tatttacttg attaatcgct tgtggtgcgc ctacacttct
     5881 taattcatgc ggacggtcac ttgttaaaac gattaatgga attctactaa tttggctttc
     5941 agcaattgca ggcgtataat tcgctgctgc tgtacctgac gtacataata tagcgacagg
     6001 tctttcactg cctttaatta acccaactgc aaaaaatgct gcacttcgct catcggggtg
     6061 tatccatgtt ttaatatttg gatgtgcttc aaatgcaagt gcaagtggcg ttgagcgtga
     6121 tcccggactg ataactactt cccttacgcc gtatgcatat aactcagatg caaatgtaaa
     6181 aacttgcttc gttaaagctg ctttatgatt tcccattcat atcgactcct aatgcattca
     6241 tcataggtgt gaacttaagg ttcgtttctg ccaattcact atctggatca gaatctttaa
     6301 caatgccaca cccagcaaat aaagttgctt gtgctttctt aataagcatc gaacgaattg
     6361 caacaataaa ttcacaatca tcatatatat ctatatagcc aaccggcgca ccatataatc
     6421 ctcgtgtacc aaattctttc tgttcaataa aatccatcgc aaattctttt ggatagccac
     6481 ctaaagcagg tgttggatgt aaatgatcaa ttaaactaat atacgaatca tccttcagtg
     6541 gcgcctttat ttcagtgtac aagtgatata aatgatcatt ttttagaatt ttaggcgtct
     6601 tatcataatg taattcagtg atataaggtt taatatcatg taaaatactg tcaacaacaa
     6661 atcgatgttc gattaagttt ttattatctt ttaaaaatgc ttcaacattt tttgtatctt
     6721 cgtcctcatc ttgtgaacgt ttaattgtac ctgctacagc tttagtcgat agtattttat
     6781 tattgacctt tattaattgt tcaggtgttt gtgaaaagaa aata
//
LOCUS       DAHHSC010000128         6807 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.129, whole genome shotgun sequence.
ACCESSION   DAHHSC010000128 DAHHSC010000000
VERSION     DAHHSC010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 6807)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6807)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6807
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.129"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            complement(122..397)
                     /locus_tag="NBI57_001897"
     CDS             complement(122..397)
                     /locus_tag="NBI57_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HCD1822832.1"
                     /translation="MENKFVPGILIGAVIGGAISLADKSTRQALVQSVKDAKNGNRTR
                     KPSKVSKIKDEVLYWKDVVEEIRRNNPELERSLKDAKETFVNRKNQR"
     gene            complement(404..868)
                     /locus_tag="NBI57_001898"
     CDS             complement(404..868)
                     /locus_tag="NBI57_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphotyrosine protein
                     phosphatase"
                     /protein_id="HCD1822833.1"
                     /translation="MVDVAFVCLGNICRSPMAEAIMRQRLKDRNIHDIKVHSRGTGSW
                     NLGEPPHEGTQKILNKHNIPFDGMISELFEATDDFDYIVAMDQSNVDNIKSINPNLKG
                     QLFKLLEFSNMEESDVPDPYYTNNFEGVYDMVLSSCDNLIDYIVKDANLKEG"
     gene            1015..1215
                     /locus_tag="NBI57_001899"
     CDS             1015..1215
                     /locus_tag="NBI57_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1128 family protein"
                     /protein_id="HCD1822834.1"
                     /translation="MTNEEKVLAIREKLNIVNQGLLDPEKYKNANEEELTDIYDFVQS
                     RERLSPSEVTAIADALGQLRHD"
     gene            1218..2474
                     /locus_tag="NBI57_001900"
     CDS             1218..2474
                     /locus_tag="NBI57_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HCD1822835.1"
                     /translation="MGILTNYKEKLQQYAELLVKVGMNVQPKQPVFIRSSVETLELTH
                     LIVEEAYHCGASDVRVVYSDPTLKRLKFENESVEHFANHEIKSYDVEARMDYVKRGAA
                     NLALISEDPDLMDGIDSQKLQAFQQQNARAFKGYMESVQKNQFPWVVAAFPSKAWAKR
                     VYPELSVEEAYIKFIDEVFDIVRIDGNDPVENWRQHIANLSVYAQKLQQKNYHALHYV
                     SEGTDLTVGLAKNHIWEDATSYVNGEEQAFIANIPTEEVFTAPDRNRVDGYVTNKLPL
                     SYNGTIIDQFKLMFKDGEIIDFSAEKGEAVLKDLINTDEGSRRLGEVALVPDDSPISN
                     RNTIFYNTLFDENAACHLAIGSAYAFNIQGGTEMTVEEKIASGLNDSNVHVDFMIGSS
                     DLTIYGIFEDGSKELVFENGNWASTF"
     gene            2564..3094
                     /locus_tag="NBI57_001901"
     CDS             2564..3094
                     /locus_tag="NBI57_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HCD1822836.1"
                     /translation="MTNQDRPMKSMSESKCYKNRQVFPQDTNHHHTMFGGTLMANIDE
                     IAAITAMKHAGAQVVTASTDSVDFLKPIKTGDILQYVAMVSYAGTSSMEVVVQIRIDD
                     VFNNKHDLAALSYLTFVALDDEGKPKHVPGVYPEDDVEKWFYDTAPQRVERRKARRIE
                     SKQTIEYLAQVQHIRD"
     gene            complement(3354..4505)
                     /gene="yfkAB"
                     /locus_tag="NBI57_001902"
     CDS             complement(3354..4505)
                     /gene="yfkAB"
                     /locus_tag="NBI57_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001089599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM/CxCxxxxC motif protein YfkAB"
                     /protein_id="HCD1822837.1"
                     /translation="MNSVKLKQPVSIYNDPWESYNDVKEHGQLTLSNIEFTTTNLCNM
                     RCSHCAVGYTLQTVDPEPLDMDLIYRRLDEIPNLRTMSITGGEPMFSKKSIRNVVKPL
                     LKYAHHRGIYTQMNSNLTLPQDRYLDIAEYIDVMHISHNWGTTDEFANVGFGAMKKQP
                     PLKAKLKLYEQMISNARTLSEQGMFVSAETMLNQSTLPHLRKIHQEVVHDMKCSRHEI
                     HPMYPADFASQLNVLTLAEMKKTIHDILDFRDEDIWMLFGTLPVFPCLKDDEDQKLLS
                     RLRNANNVTTRNDPDGRSRLNVNVFTGNVIVTDFGDETGTISNIQKDKLTDVFDKWLS
                     SDLAKSLNCHCSEFSCLGPNVLVKNMYYPNMDFKDNERHMHKQPQIIQF"
     gene            4729..4890
                     /locus_tag="NBI57_001903"
     CDS             4729..4890
                     /locus_tag="NBI57_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1822838.1"
                     /translation="MNEQQTIEQIKARLNKFIEDIDHVNPDEVRVEDIDEWIGLLDQL
                     EEKVKLVSK"
     gene            5021..5536
                     /locus_tag="NBI57_001904"
     CDS             5021..5536
                     /locus_tag="NBI57_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HCD1822839.1"
                     /translation="MTKKVAIILANEFEDIEYSSPKEALENAGFNTVVIGDTANSEVV
                     GKHGEKVTVDVGIAEAKPEDYDALLIPGGFSPDHLRGDTEGRYGTFAKYFTKNDVPTF
                     AICHGPQILIDTDDLKGRTLTAVLNVRKDLSNAGAHVVDESVVVDNNIVTSRVPDDLD
                     DFNREIVKQLQ"
     gene            5876..6685
                     /gene="sgtB"
                     /locus_tag="NBI57_001905"
     CDS             5876..6685
                     /gene="sgtB"
                     /locus_tag="NBI57_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional peptidoglycan glycosyltransferase
                     SgtB"
                     /protein_id="HCD1822840.1"
                     /translation="MKRSDRYSNSNEHFEHMKHEPHYNTYYQPVGKPPKKKKSKRILL
                     KILLTILIIIALFIGIMYFLSTRDNVDELRKIENKSSFVSADNMPEYVKGAFISMEDE
                     RFYNHHGFDLKGTTRALFSTISDRDVQGGSTITQQVVKNYFYDNDRSFTRKVKELFVA
                     HRVEKQYNKNEILSFYLNNIYFGDNQYTLEGAANHYFGTTVNKNSTTMSHITVLQSAI
                     LASKVNAPSVYNINNMSENFTQRVSTNLEKMKQQNYINETQYQQAMSQLNR"
BASE COUNT         2403 a          999 c         1181 g         2224 t
ORIGIN      
        1 aaaacaaagg caatactgaa acaatgtagt ttggccaatc attgtattat catttaataa
       61 tgataactat gaaaggcgta caccaaattg ttaagtattg ccttataaaa acaaaatcat
      121 cttagcgttg attttttcta ttaacaaatg tttctttcgc atcctttaat gaacgttcta
      181 attcaggatt attacgacga atttcttcaa caacatcttt ccagtataaa acttcgtctt
      241 taatcttgct gactttagaa ggcttacgag tgcggttacc attttttgca tctttaactg
      301 attgaactaa agcttgacgt gtagatttat cagctaaact aattgcacca ccaattacgg
      361 caccaattaa aataccagga acaaatttat tttccataaa aaactacccc tctttcaaat
      421 ttgcatcttt tacgatgtag tctattaaat tatcacaaga tgataatacc atgtcgtata
      481 caccttcaaa attattcgtg tagtatggat ctggtacatc actctcttcc atattactaa
      541 attctaacag tttgaacaat tgtcccttaa gattaggatt gatagattta atattatcaa
      601 cgttactttg atccatagcc acaatgtaat caaaatcatc tgtcgcttcg aataattcac
      661 taatcatgcc atcaaatgga atattgtgtt tgttgagaat tttttgtgta ccttcatgag
      721 gcggctctcc taaattccag ctaccagtac ctcttgaatg tactttaata tcatgaatat
      781 ttctgtcttt aagtctttgt cgcatgattg cttctgccat tggagaacga catatattgc
      841 caagacagac aaatgctaca tctaccattt tgattcctcc aaactatgta gttatatccc
      901 cattttatag cgactttaaa caataagaaa gcagattata taaaattcta ttaaagttta
      961 ttaaattgtg atactttgat aacataacta ttattagagg tgaacattgt ggctatgaca
     1021 aatgaagaga aagtattagc tattagagag aagttaaata ttgttaatca aggattatta
     1081 gatcctgaaa aatataaaaa tgcaaatgaa gaagaattaa cagatatata tgattttgtt
     1141 caatcaagag aaagattgtc gccaagtgaa gtgacagcta ttgctgacgc tttaggacaa
     1201 ttgcgacacg actaggagtg ggaattttga ctaattacaa agaaaagtta caacaatacg
     1261 ctgaactatt agttaaggtt ggtatgaatg ttcaaccaaa acagccagta tttattagat
     1321 catcagtaga aacattagaa ttaacgcatt taattgtaga agaagcatat cattgtggcg
     1381 cttcagatgt acgtgttgta tatagcgatc caaccttaaa acgcttaaaa tttgaaaatg
     1441 aatctgtgga acattttgct aatcatgaga taaaatcata tgatgttgaa gcgagaatgg
     1501 attacgttaa acgtggcgct gctaatttag cacttattag tgaagatcct gatttaatgg
     1561 atggtatcga tagccaaaaa ttacaagcat ttcaacaaca aaacgcacgc gcatttaaag
     1621 gatatatgga aagtgtacaa aaaaatcaat ttccatgggt cgtagctgct tttccatcta
     1681 aagcttgggc gaagagagta tatcctgaac tatctgtaga agaagcatat attaagttta
     1741 ttgacgaggt atttgatatt gtgcgaattg atggtaacga tccagttgaa aattggcgtc
     1801 aacacatcgc taatttaagt gtttacgctc aaaagttaca acaaaaaaat taccatgcgt
     1861 tgcattatgt atctgaaggt actgatttaa cagtaggttt agcgaaaaat catatctggg
     1921 aagatgctac aagttatgtt aacggcgagg aacaagcttt tattgctaat ataccaactg
     1981 aagaagtatt tacagcacca gatcgaaata gagtagatgg atatgttact aataaattgc
     2041 ctttaagtta taatggaacg attatcgatc aatttaaatt gatgtttaaa gatggagaaa
     2101 ttattgattt ttcagctgaa aaaggtgaag cggtattgaa agatttaatc aataccgatg
     2161 aaggttcaag aagattaggt gaagtagcgt tagtacctga tgattcaccg atatcaaatc
     2221 gtaataccat tttttacaat actttatttg atgaaaatgc agcatgtcat ttagcgattg
     2281 gatctgctta cgcctttaat attcaaggtg gaacggaaat gactgttgag gaaaagattg
     2341 caagcggatt aaatgattca aacgtacatg tcgatttcat gattggtagt agtgatttga
     2401 ctatttatgg catattcgaa gatggttcaa aagaactagt atttgaaaat ggaaattggg
     2461 catcaacatt ttaataaatg ttattttgag gtgctgagta ggaaatgaaa catgtattta
     2521 aaggtcaaat acgtgattaa agtatagatt gggagataaa ataatgacaa atcaggacag
     2581 accaatgaaa tctatgtcag aatcaaaatg ttataaaaat agacaagttt tccctcaaga
     2641 tacgaatcac catcatacaa tgtttggtgg tacattgatg gctaatattg atgaaattgc
     2701 agcaatcaca gctatgaaac atgctggtgc gcaagtagtt accgcatcta cagactcagt
     2761 agatttctta aagccgatta aaacagggga catattacaa tacgtagcga tggtttcata
     2821 cgctgggact agttcaatgg aagtggtcgt tcaaattaga attgacgatg tatttaataa
     2881 caagcatgat ttagctgcat taagttactt aacatttgtt gcgttagatg atgaaggcaa
     2941 acctaaacat gtacctggtg tatatccaga agatgatgtt gaaaaatggt tttatgatac
     3001 agcaccacaa cgtgttgaac gaagaaaagc acgtcgcatt gaaagtaaac aaacaattga
     3061 atatttagct caagtgcaac atattagaga ctaaaaatat aaagacagat tgttaaggat
     3121 gaattgaact gttatgttcg aatattccac ctacaatctg tctttttgat tatgcacgtt
     3181 tatgctttta gttaaaattt aaccttttta ttatagacta ttagtcctat taaatgacct
     3241 ttattattaa agtagagtac tgccagcaat taattgtttt ttattttata atagcattaa
     3301 aatatgcgga gactacttat cgataaagtg cttctccgca taattaagag tttttaaaat
     3361 tgtataattt gtggttgttt gtgcatatga cgctcattat ctttaaaatc catattcgga
     3421 tagtacatat ttttaacaag aacatttggt cctaaacaac taaactcgga acaatgacaa
     3481 tttaatgatt tagcaagatc agaggataac catttatcaa atacatctgt taatttatct
     3541 ttttgtatat tcgaaattgt acctgtttca tctccgaaat cagttacgat tacattacct
     3601 gtaaatacat tgacatttaa acgactacgg ccatccgggt catttctagt cgttacattg
     3661 ttagcatttc ttaaacgtga tagtaacttt tgatcttcat catcctttaa gcatggaaac
     3721 acaggcaaag taccaaataa catccaaata tcttcatctc tgaaatccaa tatatcatga
     3781 attgtctttt tcatttccgc tagagttaac acatttaatt gacttgcaaa gtcagctgga
     3841 tacatagggt gaatctcgtg tctgctacat ttcatatcat gaacgacttc ttgatgtatt
     3901 tttcgtaaat gtggtagcgt actttgattg agcattgttt ccgcagatac aaacattcct
     3961 tgttctgata atgtacgtgc attcgaaatc atttgttcat ataattttaa cttagctttt
     4021 aacggtggtt gcttcttcat tgcgccaaag ccaacatttg cgaattcatc agttgttccc
     4081 cagttatgtg agatatgcat aacatcgata tattcagcaa tatctaaata acgatcttga
     4141 ggcaatgtta ggtttgaatt catttgtgta tatatacctc gatgatgtgc atactttaat
     4201 agaggtttaa caacatttct aatagacttt ttagaaaaca ttggttcgcc acctgtaatt
     4261 gacatcgttc gcagatttgg aatttcatca agtctacgat aaattaagtc catatctaaa
     4321 ggctcggggt cgacagtttg taaagtataa ccaactgcac agtggctaca acgcatatta
     4381 caaagatttg tagttgtaaa ttcgatgtta cttaaagtta attggccatg ttctttaaca
     4441 tcgttatatg attcccatgg atcattgtaa atgctaacag gttgcttcaa tttgacgcta
     4501 ttcatcgtct taaacttcct tctttctatt tggttcatat cggtatgata ccacttcgta
     4561 ttatcactcg taacaaatta tctcttaatt ttagttaatt gaaaagggga tttttaaaag
     4621 tcaaaataat ttgaatgtca taaatatagc taagattata tcgctttaac gcaatttctg
     4681 ttaggataat aatgaaaaag aaaacaaaat aaaaggagtt aactttttat gaatgaacag
     4741 caaacaatcg aacagataaa agcacgttta aataagttta ttgaggatat cgatcatgta
     4801 aatcctgatg aagtacgtgt tgaagatata gatgaatgga ttggattgtt agatcagctt
     4861 gaagaaaagg ttaaattagt atctaagtaa atgccacata agatgtgtta gagttagata
     4921 tcgcgatagt cgcttaatat tttttatatc ttgtatgttt aatcactgat ttacttgtct
     4981 atagtaatta tatcaatgat atatagaaat gtgattttca atgactaaaa aagtagcaat
     5041 tattctagca aacgaatttg aagatataga atattcaagt cctaaagagg cattagagaa
     5101 tgctggattt aatactgtag tgattgggga tactgcaaat agtgaagttg ttggtaaaca
     5161 cggtgaaaaa gttactgtcg atgtaggcat tgcagaagct aaaccagaag attatgatgc
     5221 attattaatt cctggaggat tttcaccaga tcatttacgt ggagatacag aaggtcgata
     5281 tggcacattt gctaaatact ttactaaaaa tgatgtacca acatttgcca tttgtcatgg
     5341 gccacaaata ctaatagata cagatgattt aaaaggtcgt acgttaacag cagtattgaa
     5401 cgtgcgcaaa gatttatcaa atgcaggcgc acatgtagtt gatgagtcag tagttgtaga
     5461 caacaatatt gtaacaagtc gagtaccaga cgatttagat gattttaatc gagaaatcgt
     5521 taaacaatta caatagaagt attgtggtta tcgattgtta aaatttatat attttcgttt
     5581 gaaatgaaat atggatttta gttttaataa atgaataaca aataaaaggt cgtctctatt
     5641 ggcatttaat agggatggtc ttttatttta caaaatataa caatatttgg catgattaat
     5701 atttaaaatt gtaatgattt atctataatc ttttcaaaaa acaaaaaagt aagtatgatg
     5761 gcgtatacga ttgtaagccc ttggactgat tttcccaaaa agtgtagcct atttgtgata
     5821 gtactggtaa actcaaggta tatactaagt gagttttaaa agaaggagca aacgcatgaa
     5881 aagaagcgat aggtactcaa actcaaatga acattttgag catatgaaac acgaacctca
     5941 ctataatacg tattatcaac cagttggcaa accgccgaaa aagaaaaaaa gtaaacgaat
     6001 actattaaaa atattattaa ccattctaat cattatcgca ttgtttattg gtatcatgta
     6061 ttttttatct acacgcgata atgtggatga actaagaaaa attgaaaata aaagtagttt
     6121 tgtgtcagct gataacatgc cagagtatgt taaaggtgcc tttatttcaa tggaagatga
     6181 acgattctac aatcatcatg gattcgattt gaaaggtaca actagagctt tattttcaac
     6241 gattagcgac agagatgtgc aaggtggtag taccattaca caacaagttg tcaaaaatta
     6301 tttttatgat aatgatcgtt catttactag aaaagtaaaa gaattatttg tagctcatcg
     6361 agttgaaaaa caatataata agaacgaaat tttaagcttt tatttaaata atatttactt
     6421 tggggataat caatatacgc ttgagggcgc agcaaaccat tactttggaa caaccgtgaa
     6481 taaaaatagt acaacaatgt ctcacataac agttttacaa agcgctattt tagctagtaa
     6541 agtcaatgca cctagcgtat ataatatcaa taatatgtca gagaatttta cgcaacgtgt
     6601 aagcacgaac ttagaaaaaa tgaagcaaca aaattatatc aatgaaacac aatatcaaca
     6661 ggctatgtca caattaaatc gttaaatact tgcattcatg tgtaagatgc ttggaactat
     6721 aatgtagaag gtttaattga aatgtcataa tgataaagtt gaattgtttc tgcttaataa
     6781 attttacaga tttgcttgta atcacac
//
LOCUS       DAHHSC010000129         6727 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.130, whole genome shotgun sequence.
ACCESSION   DAHHSC010000129 DAHHSC010000000
VERSION     DAHHSC010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 6727)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6727)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6727
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.130"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            132..533
                     /locus_tag="NBI57_001906"
     CDS             132..533
                     /locus_tag="NBI57_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HCD1822841.1"
                     /translation="MKKVNNDTVFGILQLETLLGDINSIFSEIESEYKMSREEILILL
                     TLWQKGSMTLKEMDRFVEVKPYKRTRTYNNLVELEWIYKERPVDDERTVIIHFNEKLQ
                     QEKVELLNFISDAIASRATAMQNSLNAIIAV"
     gene            complement(656..1219)
                     /locus_tag="NBI57_001907"
     CDS             complement(656..1219)
                     /locus_tag="NBI57_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCD1822842.1"
                     /translation="MKLERILPFSKTLIKQHITPESIVVDATCGNGNDTLFLAEQVPE
                     GHVYGFDIQDLALENTRDKVKDFNHVSLIKDGHENIEHHINDAHKGHIDAAIFNLGYL
                     PKGDKSIVTKPDTTIQAINSLLSLMSIEGIIVLVIYHGHSEGQIEKHALLDYLSTLDQ
                     KHAQVLQYQFLNQRNHAPFICAIEKIS"
     gene            complement(1216..2169)
                     /locus_tag="NBI57_001908"
     CDS             complement(1216..2169)
                     /locus_tag="NBI57_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500380.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HCD1822843.1"
                     /translation="MGNHFQYAFENKRYHTWNYHLKNKFGQKIFKVALDGGFDCPNRD
                     GTVAHGGCTFCSAAGSGDFAGNRADSIAVQFKEIKEKMHEKWHEGKYIAYFQAFTNTH
                     APVEVLKEKFEPVLKEPGVVGLSIGTRPDCLPDDVVEYLADLNQRTYLWVELGLQTIH
                     QSTSDLINRAHDMKTYYDGVAKLRKHNINVCTHIINGLPGEDYDMMMATAKEVAQMDV
                     QGIKIHLLHLLKGTPMVKQYDKGLLTFMTQEEYTNLVVDQLEVIPPEMIVHRITGDGP
                     IDIMVGPMWSVNKWEVLNGIDAELARRNSYQGLRYKSKVKQ"
     gene            2279..3460
                     /locus_tag="NBI57_001909"
     CDS             2279..3460
                     /locus_tag="NBI57_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCD1822844.1"
                     /translation="MNIPKSVWWLVIGMALNITGSSFLWPLNTIYMKQELGKSLTVAG
                     LVLMINSFGMVIGNLLGGSLFDKLGGYKTILIGTFTCLCSTTLLNFFHGWPWYAVWLV
                     MLGFGGGMIIPAIYAMAGAVWPNGGRQTFNAIYLAQNIGVAVGAAMGGFVAEFSFNYI
                     FLANLIMYVVFALVAVTQFNIEINAKVKYPTHLDITGKKNKARFISLVLICAMFAICW
                     VAYIQWESTIASFTQSINISMAQYSVLWTINGIMILVAQPLIKPVLYLLKGNLKKQMF
                     VGIIIFMLSFFVTSFAENFTIFVVGMIILTFGEMFVWPAVPTIANQLAPDGKQGQYQG
                     FVNSAATVGKAFGPFLGGVLVDAFNMRMMFIGMMLLLVFALILLMVFKENNTQPKKID
                     A"
     gene            3748..6165
                     /locus_tag="NBI57_001910"
     CDS             3748..6165
                     /locus_tag="NBI57_001910"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HCD1822845.1"
                     /translation="MLNYNHNQIEKKWQDYWDENKTFKTNDNLGQKKFYALDMFPYPS
                     GAGLHVGHPEGYTATDIISRYKRMQGYNVLHPMGWDAFGLPAEQYALDTGNDPREFTK
                     KNIQTFKRQIKELGFSYDWDREVNTTDPEYYKWTQWIFIQLYNKGLAYVDEVAVNWCP
                     ALGTVLSNEEVIDGVSERGGHPVYRKPMKQWVLKITEYADQLLADLDDLDWPESLKDM
                     QRNWIGRSEGAKVSFDVDNTEGKVEVFTTRPDTIYGASFLVLSPEHALVNSITTDEYK
                     EKVKAYQTEASKKSDLERTDLAKDKSGVFTGAYAINPLSGEKVQIWIADYVLSTYGTG
                     AIMAVPAHDDRDYEFAKKFDLPIIEVIEGGNVEEAAYTGEGKHINSGELDGLENEAAI
                     TKAIQLLEQKGAGEKKVNYKLRDWLFSRQRYWGEPIPVIHWEDGTMTTVPEEELPLLL
                     PETDEIKPSGTGESPLANIDSFVNVVDEKTGMKGRRETNTMPQWAGSCWYYLRYIDPK
                     NENMLADPEKLKHWLPVDLYIGGVEHAVLHLLYARFWHKVLYDLGIVPTKEPFQKLFN
                     QGMILGEGNEKMSKSKGNVINPDDIVQSHGADTLRLYEMFMGPLDAAIAWSEKGLDGS
                     RRFLDRVWRLMVNEDGTLSSKIVTTNNKSLDKVYNQTVKKVTEDFETLGFNTAISQLM
                     VFINECYKVDEVYKPYIEGFVKMLAPIAPHIGEELWSKLGHEESITYQPWPTYDEALL
                     VDDEVEIVVQVNGKLRAKIKIAKDTSKEEMQEIALSNDNVKASIEGKDIMKVIAVPQK
                     LVNIVAK"
     gene            6187..6498
                     /locus_tag="NBI57_001911"
     CDS             6187..6498
                     /locus_tag="NBI57_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HCD1822846.1"
                     /translation="MKSITTDELKNKLFESKPVQIVDVRTDEETAMGYIPNAKLIPMD
                     TIPDNLNSFNKNEIYYIVCAGGVRSAKVVEYLEANGIDAVNVEGGMHAWGDEGLEIKS
                     I"
BASE COUNT         2259 a          962 c         1296 g         2210 t
ORIGIN      
        1 tagaaagcaa gtttatttac acttgtagtt ttatgcataa gttagcacat acaagttttg
       61 ggattgttgg gatgtttgtt aatacttgta tggtagctaa atatgtgatt attaattggg
      121 agatgtttag catgaaaaaa gtaaataacg acactgtatt tgggattttg caattagaaa
      181 cacttttggg tgacattaac tcaattttca gcgagattga aagcgaatac aaaatgtcta
      241 gagaagaaat tttaatttta ctaactttat ggcaaaaagg ttctatgacg cttaaagaaa
      301 tggacagatt tgttgaagtt aaaccgtata agcgtacgag aacgtataat aatttagttg
      361 aattagaatg gatttacaaa gagcgtcctg ttgacgatga aagaacagtt attattcatt
      421 tcaatgaaaa gttacaacaa gagaaagtag agttgttgaa tttcatcagt gatgccattg
      481 caagtagagc aacagcaatg caaaatagtt taaacgcaat tattgctgtg taagtttaat
      541 agcataaaaa gaggttttca ttaagttgaa aacctctttt tgttgttgtt ggtattaatt
      601 tttcaaatgt tgactactca atcctaaatt ataaatagta tagcgcagca aatgcttaag
      661 aaattttttc tatggcacaa atgaatggag catgattacg ttggtttaaa aattgatatt
      721 gcaaaacttg cgcatgcttt tgatccaaag tactcaagta atcaagcaat gcatgcttct
      781 caatttgtcc ttcgctatga ccatgatata taacaagtac aataatacct tcaattgaca
      841 ttaatgatag caatgaatta atagcttgga ttgtcgtgtc aggctttgtc acgatagatt
      901 tatcaccttt aggcaaataa cctaggttaa agatggctgc atcaatatga cctttatgtg
      961 catcatttat atgatgttca atattttcat gtccatcttt tattaaagaa acatgattga
     1021 aatccttaac tttatcacgt gtattttcca aagctaaatc ttgaatgtcg aaaccataaa
     1081 catgtccttc tggtacttgt tcggctaaaa ataaagtgtc attgccgtta ccgcaagttg
     1141 cgtccacaac aatactttct ggtgttatat gttgtttaat aagtgttttt gaaaaaggga
     1201 gtatacgttc taatttcatt gcttcacctt agacttgtaa cgcaagcctt gataagaatt
     1261 tctacgtgct aattcagcat cgatgccatt taatacttcc catttattaa cactccacat
     1321 tggacctacc atgatatcta ttggaccatc accggtaatt cggtgaacga tcatttcagg
     1381 gggaatcact tctaattggt ccacaactag gtttgtgtac tcttcttgag tcataaaagt
     1441 taataaacct ttatcgtatt gttttaccat cggtgtacct tttaacaaat gaagtaaatg
     1501 aattttaata ccttgtacat ccatttgtgc aacttctttg gcagtagcca tcatcatgtc
     1561 atagtcttcg ccaggtaagc cattaatgat gtgtgtacat acattgatat tatgcttacg
     1621 taattttgcc acaccatcat aataagtttt catatcatgg gcacgattga ttaaatcaga
     1681 tgttgactga tggattgttt gtagtcctaa ttcaacccat aagtatgttc gttgattcaa
     1741 atctgctaaa tattcgacaa catcgtctgg tagacagtca ggacgcgtac caatagataa
     1801 tcctacaaca cccggttctt taagtacagg ttcgaatttt tcttttaata cttcaactgg
     1861 tgcatgtgta tttgtaaatg cctgaaaata agcaatatat tttccttcgt gccatttctc
     1921 atgcatcttt tccttaattt ctttaaattg tactgcgatt gaatctgcac gattacctgc
     1981 aaagtccccg ctacctgcag cagaacaaaa tgtacatcca ccatgtgcta cagtgccatc
     2041 gcggttagga cagtcaaacc cgccatccaa tgcaacttta aatatttttt gtccaaattt
     2101 attttttaaa tggtaattcc atgtgtgata acgtttgttt tcaaaagcgt attggaaatg
     2161 attgcccata tgtcattttc ctttctataa aaaaagagtt ctaagtacag attttaacat
     2221 attttaatgt tatagtgttt attatagttt gacaaaaaag agagaggaac tatgaaatat
     2281 gaatatacct aaatcagtct ggtggctagt aattggcatg gcgttaaata ttactggttc
     2341 cagttttttg tggcctttaa atacaattta tatgaaacaa gaacttggaa aaagtttaac
     2401 tgttgctggt ttagtgctaa tgataaattc atttggcatg gttattggaa acttattagg
     2461 tggttcacta tttgataaat taggtggata caagacgatt ttaattggaa ctttcacttg
     2521 tctttgtagt acaacgctac ttaatttctt tcacgggtgg ccttggtatg ctgtatggct
     2581 tgtaatgtta ggctttggtg gcggaatgat tattcctgcg atatacgcta tggctggagc
     2641 agtgtggccg aatggcggaa gacaaacgtt taatgcgata tacttagcgc aaaatattgg
     2701 tgtggctgtc ggtgctgcaa tgggcggctt tgtcgcagaa tttagcttta actatatctt
     2761 tttagccaat cttattatgt atgttgtgtt tgcgcttgtc gcggtaacgc aatttaatat
     2821 tgaaattaat gcgaaagtta aatatccaac tcatttagat attactggta aaaagaataa
     2881 agcaagattt atttcattag tactaatttg tgcaatgttt gcaatttgtt gggttgcgta
     2941 tattcaatgg gagtctacaa tcgcatcatt tacacaatct attaatattt caatggcaca
     3001 atatagtgtt ttatggacaa ttaacggaat aatgatttta gtagcacaac cattaattaa
     3061 accggttctc tatctgttaa aaggaaactt aaagaagcaa atgtttgtcg gcatcatcat
     3121 ttttatgttg tcgttctttg tcacgagttt tgccgaaaac tttacaatat ttgttgtcgg
     3181 tatgattatt ttaacttttg gagaaatgtt tgtatggcca gcagttccaa ctatagccaa
     3241 tcagttagcg ccagatggta agcaaggaca gtaccaaggt tttgtgaatt cagctgctac
     3301 agtaggaaaa gcatttggtc catttcttgg tggtgtatta gttgatgcgt ttaatatgcg
     3361 catgatgttt atcggtatga tgctactact tgtatttgca ttaatattat taatggtttt
     3421 caaggagaat aatacgcaac ctaaaaaaat agatgcataa tgagtaaata gaattaacgt
     3481 tatagacttg aaataaatgt cgttataaca taatattaat ttgtataatt taatttcgtt
     3541 tggagctttt ctacagaaag ctagtgatgc tgagagctag tgttaaggac taaatgtaaa
     3601 tcgtattaat tttaaattga atgaatgaca tctcttacta ttaaaatgag tgcacaattt
     3661 ttgtgaaata gggtggtaac gcggcaaatg tcgtccctat gtaaatagaa tagttagagg
     3721 tgtctttttt attgaatagg aggaaatgtg ttgaattaca accacaatca aattgaaaag
     3781 aaatggcaag actattggga cgaaaataaa acatttaaaa caaatgataa cttaggtcaa
     3841 aagaaatttt atgctttaga catgtttcca tatccatcag gtgctggttt acatgttgga
     3901 catcctgagg gctatacagc aacagatatc atttcaagat ataaaaggat gcaaggttat
     3961 aatgtattac atccgatggg gtgggatgca ttcggattac cagcagagca atatgcttta
     4021 gacactggca acgacccacg tgaatttaca aagaaaaata tccaaacttt taaacgacaa
     4081 attaaagaat tagggttcag ttatgattgg gatcgtgaag ttaatacaac agatccagaa
     4141 tactataaat ggacacagtg gattttcata cagttatata acaaaggttt agcatacgtt
     4201 gatgaagttg cagttaactg gtgtccagca ttaggcactg ttttatctaa cgaagaagtg
     4261 attgatggtg tctctgaacg tggtggacat ccagtttatc gtaagccgat gaaacaatgg
     4321 gtacttaaaa tcacagaata tgcagatcaa ttattagcag atttagatga tttagattgg
     4381 cctgagtctt taaaagatat gcagcgcaat tggattggac gttctgaagg ggccaaagtt
     4441 tcatttgatg tagataatac ggaaggaaaa gtagaagtat ttacgactag accagacact
     4501 atctatggtg catcattctt agtcttaagt cctgaacatg cattagttaa ttcaattaca
     4561 actgatgaat ataaagaaaa agtaaaagct tatcaaacag aagcttctaa aaagtcagat
     4621 ttagaacgta cagatttagc aaaagataaa tcaggtgtgt ttactggtgc atatgcaatt
     4681 aatcctttat ctggtgaaaa agtacaaatt tggattgctg attatgtatt atcaacatat
     4741 ggtactggag caattatggc agtaccagcg catgatgaca gagattatga atttgctaaa
     4801 aagtttgatt tgccaatcat tgaagtcatc gaaggtggaa atgttgaaga agcagcatac
     4861 actggtgaag gtaaacatat taattctggt gaacttgatg gtttagaaaa tgaagcggca
     4921 attactaaag ctattcaatt attagagcaa aaaggtgctg gcgaaaagaa agttaattac
     4981 aaattaagag attggttatt cagtcgtcag cgttattggg gcgaaccaat tcctgtcatt
     5041 cattgggaag atggaacaat gacaactgtt cctgaagaag agctaccatt gttgttacct
     5101 gaaacagatg aaatcaagcc atcagggact ggtgagtctc cactagctaa tattgattca
     5161 tttgtaaatg ttgtagatga aaaaacaggt atgaaaggac gtcgtgaaac aaatacaatg
     5221 ccacaatggg caggtagttg ttggtattat ttacgttaca tcgatcctaa aaatgaaaat
     5281 atgttagcag atcctgaaaa attaaaacat tggttacctg ttgatttata tatcggtgga
     5341 gtagaacatg cggttcttca cttattatat gcaagatttt ggcataaagt cctttatgat
     5401 ttgggtatcg tacctactaa agaacctttc caaaaattat ttaaccaagg tatgatttta
     5461 ggagaaggta atgagaagat gagtaaatct aaaggaaatg taatcaatcc tgatgatata
     5521 gtacagtctc atggtgcaga tactttacgt ctttacgaaa tgtttatggg acctttagat
     5581 gctgcaattg catggagtga aaaaggatta gatgggtctc gtcgattctt agatcgcgta
     5641 tggcgtttaa tggtaaatga agatggaaca ttgagttcaa aaattgtaac tacaaataat
     5701 aaatctttag ataaagttta taaccaaact gttaaaaagg taacagaaga ctttgaaaca
     5761 ttaggattta atactgctat tagtcaatta atggtattta ttaatgagtg ttataaagtt
     5821 gatgaagttt ataaacctta cattgaaggc ttcgttaaaa tgttagcacc tattgcacca
     5881 catatcggtg aagaattatg gtcaaaatta ggacatgaag agtctattac gtaccaacct
     5941 tggccaactt atgacgaagc actacttgta gatgatgaag tagaaatcgt tgttcaagta
     6001 aatggtaaat tgagagctaa aattaaaatt gctaaagata catcaaaaga agaaatgcaa
     6061 gaaattgcct tatctaatga caatgttaaa gcgagtattg aaggtaaaga catcatgaaa
     6121 gtcatcgctg ttcctcaaaa attagtcaat attgtagcta aataatgttt taaggaggac
     6181 tttgaaatga agtcaattac tacagatgaa ttaaaaaaca aactttttga atctaaacca
     6241 gttcaaattg ttgatgttcg tactgacgaa gaaacagcaa tgggatatat tcctaatgca
     6301 aagttgattc caatggatac cattccggat aatttaaatt catttaataa aaatgaaata
     6361 tattatattg tatgtgctgg tggagttcga agcgctaaag ttgtagaata tttagaggca
     6421 aatggcattg atgccgtaaa tgtcgaaggc ggcatgcacg catggggcga tgaaggtttg
     6481 gaaataaaaa gtatttaaag tagtgacata atttaaaata atattacatt tgtaatgaca
     6541 ccaagtaacg tttcggttgc ttggtgtttt ttggtatgaa ttactttctg ttacaaaaca
     6601 atctaaagcg ttcttgttat gttttattaa gattttaatt acaaaacgga aactaaattg
     6661 taataaaata aaactttatt ttataaaatg atgatgataa aattgagtga acttaaaata
     6721 ttgtaca
//
LOCUS       DAHHSC010000130         6720 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.131, whole genome shotgun sequence.
ACCESSION   DAHHSC010000130 DAHHSC010000000
VERSION     DAHHSC010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 6720)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6720)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6720
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.131"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            complement(181..1059)
                     /locus_tag="NBI57_001912"
     CDS             complement(181..1059)
                     /locus_tag="NBI57_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exonuclease"
                     /protein_id="HCD1822847.1"
                     /translation="MPNKILLVDGMALLFRHFYATSLHKQFMYNSQGVPTNGIQGFVR
                     HIFSAIHEIRPTHVAVCWDMGQSTFRNDMFDGYKQNRSAPPEELIPQFDYVKEISEQF
                     GFVNIGVKNYEADDVIGTLAQQYSTDNDVYIITGDKDLLQCINDNVEVWLIKKGFNIY
                     NRYTLHRFNEEYALEPQQLIDIKAFMGDTADGYAGVKGIGEKTAIKLIQQYQSVENVV
                     ENIDALSAGQRNKINDNLDELYLSKRLAEIHTQVPIDSEALFEKMSFATTLNHILSIC
                     NEHELHVSGKYISSHF"
     gene            complement(1079..4519)
                     /locus_tag="NBI57_001913"
     CDS             complement(1079..4519)
                     /locus_tag="NBI57_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dynamin family protein"
                     /protein_id="HCD1822848.1"
                     /translation="MINKEQLDLLYKLKKEVEKSRNEALLHTINQVIKKVYLQQYTCS
                     FVGHFSAGKSTLINLLIEQDILPSSPVPTTSNTAIVSVSDNHDIIANLPNQTYAKLSN
                     YDEVREMNRQNVDVESVEINFQSAKFENGFTLQDTPGVDSNVASHQSITEQYMYTSNM
                     IFYTVDYNHVQSELNFKFMKHINDVGIPVVFIINQIDKHQDDELSFSTFKSRVEKSIA
                     DWGIKLERTFYVSKFDHPENELEVLSSYLISLDQHRETIEDYTSRTVEYITEAQLDYI
                     QSEIQEVLEDLGIEEAEFEQAFLNSQQHQAISEEAQLLNNPDELMAFLKNKRKNILEN
                     AYIMPHNMREMLRSYLESMSQDFNVGGFFNKKKKKLQIQQQRLLTATDALQEHVNQQI
                     RQPMREDMSFVTRFINKKEASDKVLNQHYDVKPEMIEDLYQPQTSISNTYVLTFSDEV
                     VKAIKKYVEQQSTPIFKEIIENVQADELPTEESDDLKEYQRYTELNELRQSLTTKNYR
                     HYYIHLDESLDKLIGRQETTYQVATDNTQDNRDNQQLNQNTATTNMSIDIQKALDIIS
                     DVPLFKRTKQDIHETLTRIDNKLIKIGVFGTFSAGKSSLINALLGEHILVSSPNPTTA
                     ATTEISYGDESYITLKSQSQLLDEINAVVEYQDISFSTIEEFINWDLEKLKSHLNKNQ
                     LAFIQAVEKHYKLYVNMLENGEKHAINQQELKKWSAEDEYATFVKTVHIALMHDWLKG
                     KIIVDSLGLHSNNQRHTNETEQILTSSDLILYVSYFNHSFTDNDKAFIEHMKDMNQLN
                     ENQAFKMVINAADLAESQDDLEAVETYVSDALRQVHLQSDIFAVSSRNALQAEDKGID
                     QLKQSIQQFVDVESKSILEQQMIHQLQQMDRSYVEMITEFETNKADISRRQQRLTDYK
                     DKQRLQHQLIDATLQHTDNEVEEQVYHLNARLKLQLLDDVKSVFNSQMTQNSDFNEEK
                     KVSTKVYLDQIHQRLFLEQSLITERIKKYFNKQLTKQIAPIVQQLADLHVIVNPQFNF
                     ESANIEQPLLHIDFNDMLNALPKQLTKRKILNPNGQRDIHESICQSTLGLLQPQMGLL
                     RQQLELYVKQMAVEAESQFESFEANIQTQINDLLAFDLDTTLINQLKDKHQQLKTILY
                     "
     gene            4823..6163
                     /locus_tag="NBI57_001914"
     CDS             4823..6163
                     /locus_tag="NBI57_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HCD1822849.1"
                     /translation="MKNTFNPLQRLHFYAAIFIAPLLITLTISGIGYLFFPEVENNIY
                     KNEFFGDSDVKTHQTLNDAVHQVEQQYEGFFVSKVSILDEPYNKRITLSDMAGNQRYV
                     FLDHNNQFVADQNAKHTYSNVMRSIHSSLFTENTIINYLVELTACWTIFMILSGTYLL
                     IKKHLISNKSKALRWQKWHAMIGVIIAIPVFVLVLTGLPWSGFMGSKIAGMMDTNGDL
                     GQGELAINPPKSDLNELPWATRKNKQPASSEKGSSGHHGNAAMPQTKLDYQISIDKVV
                     VQAQKAGIKKPFSIVYPSDKNGTFIVSNTSNSGVTGLDVSPYKEQTLYFDQYSGKKLG
                     TIKYDDYGIIAKWFTWGIPLHEGHLFGILNKIINLFVCITLLVAIGMGFVSWIKRTKN
                     TAVKVPHRVKKPASISLIICLIVLGLLMPLFGLSLILVFIIELILYIKDRRAKQ"
     gene            complement(6369..6701)
                     /locus_tag="NBI57_001915"
     CDS             complement(6369..6701)
                     /locus_tag="NBI57_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4889 domain-containing protein"
                     /protein_id="HCD1822850.1"
                     /translation="MGKKMGLGLSIALVVIGIAVVCLMIFSSQKTTYFGYMNSKTNAE
                     KVVSEKDGLVKHNIKVEPSNDFKPKKGDFVKLVSKDDGKTFYKQEIVKHDDVPHGLMM
                     KIHDMHMN"
BASE COUNT         2112 a         1094 c          970 g         2544 t
ORIGIN      
        1 ttggatgaaa tataattctt taaactctat tgattaatca aatatgtatc tatcaaaatt
       61 cgtcttattt attcactctg acatatccaa atacgcaaaa agactatttc taccttgcat
      121 cgatagaaat agtcttttta tacttaattg gtttaatttt tcataaaatt ttatactttt
      181 ttaaaaatgg gatgaaatat attttcctga aacatgtaat tcatgttcgt tacaaattga
      241 taaaatatga ttaagtgttg ttgcaaatga cattttctca aaaagcgctt cactatcaat
      301 tggtacttgt gtgtgaattt cagcaagtcg cttcgataaa tatagttcat ctaaattgtc
      361 attaatctta ttacgttgcc cagctgataa tgcatcaatg ttttcaacca cattttcaac
      421 gctttgatat tgctgaatta acttaatagc cgttttctca ccaattcctt taacacctgc
      481 atatccatct gctgtatcac ccataaacgc tttaatatcg attagttgtt gtggttcaag
      541 ggcatattct tcgttaaaac gatgtaatgt atatctatta taaatgttaa aacctttttt
      601 aattagccag acttcaacat tgtcattaat acattgcagt aaatctttgt cgcccgtaat
      661 aatatagaca tcgttatcag ttgaatattg ttgtgctaat gtacctataa catcatccgc
      721 ttcatagttt ttaacgccaa tatttacaaa gccaaattgc tctgaaattt ctttaacata
      781 atcaaattgt ggtatcaatt cttctggtgg tgcagaacga ttttgcttat aaccatcaaa
      841 catatcattt ctaaaagttg attgtcccat atcccaacat acagctacat gtgtagggcg
      901 tatttcatgt attgccgaaa agatatgacg cacaaatcct tgtattccat ttgtaggtac
      961 accttgtgaa ttgtacataa attgtttatg aagacttgta gcatagaaat gtctaaataa
     1021 tagcgccata ccatctacaa gtaatatttt attaggcatt taaaacgttc cttctttctt
     1081 aatataaaat agttttcagt tgttgatgtt tatctttcaa ttgattgata agtgttgtat
     1141 ctaaatcaaa tgctaataaa tcgtttattt gcgtttgaat attagcttca aaactttcaa
     1201 attgcgattc agcttctaca gccatttgct ttacatataa ttcaagctgt tgcctcaata
     1261 atcccatttg tggttgtaat aatcctaacg tactttgaca aattgattca tgtatatctc
     1321 tttgcccatt tggattcaaa attttacgtt ttgttaattg tttaggcaat gcatttagca
     1381 tatcattgaa atcgatgtgc aataatggtt gctctatatt agctgattca aagttaaact
     1441 gaggattaac aatgacatgt aaatctgcta attgttgaac gattggtgca atttgcttag
     1501 tgagttgctt attaaagtat ttttttatac gttctgtaat taaagattgc tctaaaaaca
     1561 atcgttgatg aatttgatct aagtatactt tcgtagacac tttcttttct tcattaaaat
     1621 cactattttg cgtcatttga gaattaaaca ctgatttaac atcgtcaagt agttgtaatt
     1681 ttaaacgtgc atttaaatga taaacttgtt cttcaacttc gttgtctgta tgttgtagcg
     1741 tcgcatcaat taattgatgt tgtaaacgct gtttatcttt ataatctgtt aacctttgtt
     1801 ggcgacgtga aatatcagct ttatttgttt caaattctgt aatcatctct acataagaac
     1861 gatccatttg ttgaagctga tgaatcattt gttgttctaa aattgattta gattcaacat
     1921 caacaaattg ttgtatgctt tgttttaatt gatcaatgcc cttatcttca gcttgcaatg
     1981 catttcgact tgatacagca aaaatgtctg attgtaagtg tacttgtctt aatgcatctg
     2041 atacatatgt ttcaactgct tcaagatcat cttgactttc tgctaaatca gcagcattaa
     2101 ttaccatttt aaatgcttgg ttttcattca actggttcat atctttcatg tgttctataa
     2161 acgctttgtc attatcagta aatgaatgat taaaataact tacatacaat attaagtctg
     2221 aagaagttaa aatttgttcg gtttcatttg tatgcctttg gttatttgag tgtagcccta
     2281 atgaatcaac aattatttta ccttttaacc aatcatgcat taatgcaatg tgtactgtct
     2341 taacaaatgt tgcatattca tcttctgcac tccacttttt caattcctgt tgattaatgg
     2401 catgtttttc tccattttcc aacatattga catacaattt ataatgtttt tcaactgcct
     2461 gaataaaagc gagttgattt ttatttaaat gtgattttaa cttttctaaa tcccaattaa
     2521 taaattcttc tatagttgaa aaagatatat cttggtattc aactactgca ttaatttcat
     2581 ctaataattg cgattgtgat tttagcgtta tataactctc gtctccataa gatatttctg
     2641 tagtagctgc tgtagtagga tttggagaac tcactaaaat atgctcgcct aataatgcat
     2701 ttatcaaact acttttaccc gcactaaatg ttccaaatac acctattttt attaatttat
     2761 tatctatacg tgttaatgtt tcgtggatat cttgctttgt acgcttgaac aaaggcacat
     2821 ccgaaattat atcaagcgct ttttgaatat ctatagacat atttgttgta gctgtatttt
     2881 gatttagctg ctgattatca cgattatcct gagtattatc agtagccact tgatatgttg
     2941 tctcttgtcg acctattaat ttatctagag attcatctaa atgaatatag tagtgacgat
     3001 aattcttagt cgtcaatgac tgacgcagct catttaattc tgtataacgt tgatattctt
     3061 ttaaatcatc actttcttct gttggtaatt catctgcctg cacattttct attatttctt
     3121 taaaaatcgg tgttgattgt tgttcaacat atttcttaat ggctttaacc acttcgtctg
     3181 aaaatgtaag tacataagta ttgctgattg atgtttgtgg ttgatataaa tcttcaatca
     3241 tttccggttt aacgtcataa tgctgattta atactttatc tgaagcttct tttttattga
     3301 taaaacgcgt tacaaatgac atatcttctc gcattggttg acgaatttgt tgattaacat
     3361 gttcttgtaa cgcatctgtc gctgttaata atcgctgttg ttgaatttgt agctttttct
     3421 tctttttatt aaaaaaccca ccaacattaa aatcttgaga catactttcc aaataacttc
     3481 gtaacatttc tctcatatta tgcggcataa tgtatgcatt ttctaaaata tttttacgct
     3541 tattctttaa aaatgccatt aattcatctg gattatttaa aagttgtgcc tcttcactaa
     3601 ttgcttgatg ttgttgacta tttaaaaatg cttgttcaaa ctccgcttct tcgataccta
     3661 aatcttctag tacttcctga atttcagact gaatgtaatc tagctgagct tcggtaatgt
     3721 attcaaccgt tcttgatgta taatcctcta ttgtctctct atgttgatct aatgaaatta
     3781 gataacttga taatacttca agttcatttt cagggtgatc aaatttagat acataaaagg
     3841 tgcgttctaa tttaatgccc caatctgcaa ttgatttttc aactcgtgat ttaaacgtag
     3901 agaatgacaa ttcatcgtct tgatgcttgt caatttgatt aatgataaac acaacaggta
     3961 ttccaacatc atttatatgc ttcataaact taaagttaag ttcagattga acgtggttat
     4021 agtcaaccgt ataaaatatc atattacttg tatacatata ttgttctgtt attgactgat
     4081 gtgatgcaac atttgaatca acacctggtg tatcttgcaa cgtaaaccca ttttcaaatt
     4141 tagctgattg aaaattaatt tctacagatt caacgtcgac attttggcga ttcatttccc
     4201 ttacttcatc ataattagat aatttggcat atgtttgatt cggcaaatta gcaataatat
     4261 cgtgattgtc tgaaactgac acaatagcag tattacttgt cgttggtaca ggtgaacttg
     4321 gtaagatatc ttgttcaatt aataaattta tcagtgtcga tttacctgca gaaaaatgtc
     4381 caacgaacga acatgtatat tgctgcaaat atactttctt aattacttgg ttaattgtat
     4441 gtaaaagtgc ttcatttcgc gacttttcaa cttctttttt taatttatat aaaagatcta
     4501 attgttcttt attaatcatg ttactttccc ctttggttcg ttcacataga aatgcttgct
     4561 tgttaaaacg aattcataca atatcgatgt ccatttaaac atagatcgtt aaatggctcg
     4621 cattgtatta tgtttaaatt ttatcatagc tgcttgataa aacatacgaa taatcaattt
     4681 tcttcaaaca ttccaactta aaagttcata tttctaaaaa tcacaaatca tcgttaaatt
     4741 tttctagaaa ataaatattg tcacagtttg ttcacacacg tcacagaatt gacaccgtat
     4801 aatagccaat tgggagggaa atatgaaaaa tacatttaat ccattacaaa gattacattt
     4861 ctatgcagca atatttattg ctccactgtt aatcacttta accatttcgg gcattggtta
     4921 cttgttcttt ccagaagttg aaaataatat ttataagaat gagttttttg gtgacagtga
     4981 tgtaaaaacg catcaaacat taaatgatgc agtacatcaa gttgaacaac aatatgaagg
     5041 attctttgta agtaaagtta gcatacttga tgaaccatat aacaaacgaa ttacactgag
     5101 tgatatggca ggaaatcaac gttacgtctt tctagatcat aacaatcaat ttgttgcaga
     5161 tcaaaatgcg aaacatacgt attctaatgt gatgcgaagt atacatagtt ctttgtttac
     5221 tgaaaatact attattaatt atttagtaga gttaaccgca tgttggacga tattcatgat
     5281 tttatctggt acttatttac tcattaagaa gcatttaatt tctaacaaaa gtaaggcact
     5341 tcgttggcaa aagtggcacg caatgattgg agttatcatt gcaattccag tatttgtatt
     5401 agttttaact ggattgccat ggtctggttt tatgggcagt aaaattgccg gtatgatgga
     5461 cacaaacggt gaccttggtc aaggtgaatt agcgattaat ccacctaaat cagatttgaa
     5521 cgaattacct tgggctacac gtaaaaataa acagccagct tcatccgaaa aaggttcaag
     5581 tggtcatcat ggtaatgcag caatgcctca aaccaaatta gattatcaaa tatctattga
     5641 taaggtcgtt gtacaagcgc aaaaagctgg tattaaaaag ccgttttcaa tcgtatatcc
     5701 aagtgataaa aatggtacct ttattgtatc taatactagt aattcaggtg ttactgggct
     5761 agatgtatca ccttacaagg aacaaacact ttatttcgac caatatagcg gtaaaaagct
     5821 aggtacgatt aaatatgatg actacggtat tattgctaaa tggtttacat ggggcattcc
     5881 gcttcacgaa ggtcatttat tcggcatttt aaataaaatc attaatttat ttgtatgtat
     5941 cactttatta gtagccattg gcatggggtt tgtctcttgg ataaagcgta caaaaaatac
     6001 tgcagtaaaa gtaccacatc gcgtaaaaaa accagcatct atatcactca taatatgttt
     6061 aattgtatta ggattattaa tgccattatt tggattatca cttatccttg tatttataat
     6121 tgaattaata ttatatatta aagatcgtcg tgctaaacaa taatgcactt aaagttttga
     6181 actgacgaaa tttacaaaat gattctcgtc tctctaatta cttaaaacgg gttctataat
     6241 aaatcgtact gatggaaagt ttttactttt tatctgtcag attttttgaa tttgaagata
     6301 aaaaagcatc taaaacgcat aagaagataa ctctacttct tcaaaattac gttatagatg
     6361 cttttttatt aattcatatg catgtcgtga attttcatca ttaaaccgtg tgggacgtca
     6421 tcatgtttaa caatctcttg tttataaaat gtcttcccat catctttaga aactaatttt
     6481 acaaagtctc cttttttcgg tttaaaatca tttgatggtt ctactttgat attatgtttg
     6541 actaatccat ctttttcact gacaactttt tctgcatttg ttttactatt catataacca
     6601 aaataagtcg ttttttgact agaaaaaatc attaaacata caacggcaat accaataaca
     6661 accaatgcta tagataaacc tagacccatt tttttaccca tttatagact cctccatttt
//
LOCUS       DAHHSC010000131         6670 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.132, whole genome shotgun sequence.
ACCESSION   DAHHSC010000131 DAHHSC010000000
VERSION     DAHHSC010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 6670)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6670)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6670
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.132"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            <1..313
                     /locus_tag="NBI57_001916"
     CDS             <1..313
                     /locus_tag="NBI57_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCD1822851.1"
                     /translation="TLLNQTYITYTKLLEQFTMEDIAAQQQVKINTIEDHVLEILIKG
                     YMSNYDDYVELEDQLQFLNFYQQHRGERLKFYKEQFDTLSYFQLKVLIVGFERGDLNV
                     A"
     gene            303..1682
                     /locus_tag="NBI57_001917"
     CDS             303..1682
                     /locus_tag="NBI57_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HCD1822852.1"
                     /translation="MLHDILRNKFGFENFKPGQQEIIESIMSQQHTLGILPTGSGKSL
                     CYQIPTYLSGKPTLIISPLISLMDDQVMQLKINGEKRVTCIHSGMDEIEKKHNIKCLL
                     HSRFIFLSPEFLLQPSNFKLISMIDFGMIVLDEAHCLSEWGYDFRPHYALIGKVTKHF
                     KEAVVLALTATAPPHLQDDLTEMLAIQFNVIKTTMNRPNISFKHLNFHDDEDKIEWLL
                     PFLQQSGPTIIYVSSKKMCLNLAQLIYDSGFLTGIYHGDMNYQERHTVQQQFLNNDIP
                     IIVATSAFGMGINKKDIRTIIHFHLSTSPSNYIQEIGRAGRDGELSQAISLFQPDDKY
                     ILETLLFADMITEEDVQNFEIGEFLAPDKQDVLTTLHSFYSIGALKQIFKQSFKRKQL
                     GFFRMIGYCKLDQCRRKYLLEFFGEYPPAQDRCCDNDSNITDIVILNKKKVIRSIGFD
                     EKLQNLFLR"
     gene            1835..3295
                     /gene="ebpS"
                     /locus_tag="NBI57_001918"
     CDS             1835..3295
                     /gene="ebpS"
                     /locus_tag="NBI57_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elastin-binding protein EbpS"
                     /protein_id="HCD1822853.1"
                     /translation="MSNNFKDDFEKNRQSIDTNSHQDHTEDVEKDQSELEHQDTIENT
                     EQQFPPRNAQRRKRRRDLATNHNKQVHNESQTSEDNVQNEAGTIDDRQVESSHSTESQ
                     EPSHQDSTPQHEEEYYNKNAFAMDKSHPEPIEDNDKHETIKDAENNTEHSTVSDKSKA
                     EQSQQPKPYFATGANQANTSKDKHDDVTVKQDKDESKDHHSGKKGAAIGAGTAGVAGA
                     AGAMGVSKAKKHSNDAQNKSNSDKSNNSTEDKASQDKSKDHHNGKKGAAIGAGTAGLA
                     GGAASKSASAASKPHASNNASQNHDEHDNHDRDKERKKGGMAKVLLPLIAAVLIIGAL
                     AIFGGMALNNHNNGTKENKIANTNKNNADESKDKDTSKDASKDKSKSTDSDKSKEDQD
                     KATKDESDNDQNNANQANNQAQNNQNQQQANQNQQQQQQRQGGGQRHTVNGQENLYRI
                     AIQYYGSGSPENVEKIRRANGLSGNNIRNGQQIVIP"
     gene            3420..3536
                     /locus_tag="NBI57_001919"
     CDS             3420..3536
                     /locus_tag="NBI57_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1822854.1"
                     /translation="MLFNKVLSRILAYQLNFLNSHQKDKTPQSTNLTRENKK"
     gene            3697..4683
                     /locus_tag="NBI57_001920"
     CDS             3697..4683
                     /locus_tag="NBI57_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpdA family putative bacillithiol disulfide
                     reductase"
                     /protein_id="HCD1822855.1"
                     /translation="MQKVESIIIGGGPCGLSAAIEQKRKGIDTLIIEKGNVVESIYNY
                     PTHQTFFSSSDKLSIGDVPFIVEESKPRRNQALVYYREVVKHHQLKVNAFEEVLTVKK
                     MNNKFTITTTKDVYECRFLTIATGYYGQHNTLEVEGADLPKVFHYFKEAHPYFDQDVV
                     IIGGKNSAIDAALELEKAGANVTVLYRGGDYSPSIKPWILPNFTALVNHEKIDMEFNA
                     NVTQITEDTVTYEVNGESKTIHNDYVFAMIGYHPDYEFLKSVGIQINTNEFGTAPMYN
                     KETYETNIENCYIAGVIAAGNDANTIFIENGKFHGGIIAQNMLAKKQTPLES"
     gene            complement(4798..5766)
                     /locus_tag="NBI57_001921"
     CDS             complement(4798..5766)
                     /locus_tag="NBI57_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HCD1822856.1"
                     /translation="MKHLLVIHTGGTISMSQDQSNKVVTNDINPISMHQDVINQYAQI
                     DELNPFNVPSPHMTIQHVKQLKDIILEAVTNKYYDGFVITHGTDTLEETAFLLDLILG
                     IEQPVVITGAMRSSNEIGSDGLYNYISAIRVASDEKARHKGVMVVFNDEIHTARNVTK
                     THTSNTNTFQSPNHGPLGVLTKDRVQFHHMPYRQQALENVNDKLNVPLVKAYMGMPGD
                     IFSFYSREGIDGMVIEALGQGNIPPSALEGIQQLVSLNIPIVLVSRSFNGIVSPTYAY
                     DGGGYQLAQQGFIFSNGSNGPKARLKLLVALSNNLDKAEIKSYFEL"
     gene            5843..6502
                     /locus_tag="NBI57_001922"
     CDS             5843..6502
                     /locus_tag="NBI57_001922"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HCD1822857.1"
                     /translation="MKAINIALDGPAAAGKSTIAKRVASELSMIYVDTGAMYRALTYK
                     YLKLNKTEDFAKLVDQTTLDLTYKADKGQCVILDNEDVTDFLRNNDVTQHVSYVASKE
                     PVRSFAVKKQKELAAEKGIVMDGRDIGTVVLPDADLKVYMIASVEERAERRYKDNQLR
                     GIESNFEDLKRDIEARDQYDMNREISPLRKADDAVTLDTTGKSIEEVTDEILAMVSQI
                     K"
     gene            6533..>6670
                     /locus_tag="NBI57_001923"
     CDS             6533..>6670
                     /locus_tag="NBI57_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1822858.1"
                     /translation="MQRLTHIQNECADNDLLKLKGDFYSMMNESCLFIFGKSSCVSEL
                     CK"
BASE COUNT         2493 a         1039 c         1190 g         1948 t
ORIGIN      
        1 tacattatta aatcaaactt atattactta tacaaaattg cttgaacaat tcacgatgga
       61 agatatagcg gctcaacaac aagttaaaat caatactatc gaagatcatg tacttgaaat
      121 cttaatcaaa ggttacatgt ctaattacga tgattatgtt gaactagaag atcaactcca
      181 gtttttgaat ttttatcaac aacatcgtgg cgaacgatta aaattttaca aagaacaatt
      241 tgacacgtta tcatattttc aattaaaagt attaatcgtt ggatttgaaa gaggtgatct
      301 gaatgttgca tgatatttta cgaaacaaat ttggcttcga gaattttaaa ccgggacaac
      361 aggaaattat agaaagtata atgtctcaac aacacactct aggtattctt ccaactggaa
      421 gtggaaagag tttgtgttat caaataccta cgtatttatc aggtaagccg acattaatta
      481 tctcaccgtt aatatcttta atggatgacc aagttatgca gttgaaaata aatggagaaa
      541 aacgtgtaac atgtattcac tctggtatgg atgaaattga gaaaaagcat aatattaaat
      601 gtttactaca tagccgcttc atctttctaa gtccagaatt tctcctgcaa ccgtcaaatt
      661 ttaaattaat atctatgata gactttggca tgattgttct agatgaagca cattgcctat
      721 ctgaatgggg atatgatttc agaccacatt atgctctaat aggaaaagta acaaagcatt
      781 ttaaagaagc ggttgtctta gcgttgacag caactgcacc accgcattta caagatgatt
      841 tgacggaaat gttagcgatt caattcaacg ttattaaaac tacaatgaat cgcccaaaca
      901 taagctttaa gcatcttaat tttcatgatg atgaagataa aattgagtgg ttgctgccgt
      961 ttctacaaca gtcgggacca acgattatct atgtctcatc gaaaaagatg tgtctgaatt
     1021 tagcgcaact tatttatgat tcaggttttc ttactggcat ttatcatggt gatatgaatt
     1081 atcaagagcg acacacagtt caacaacaat ttttaaataa tgatattccg attatagtcg
     1141 caacgagtgc ttttggtatg ggtattaata aaaaagatat tcgcacaatc attcactttc
     1201 atctttcaac aagtccttct aactacattc aagaaattgg ccgtgcaggt cgcgatggtg
     1261 aactaagtca ggcaattagt ttatttcaac cggacgataa atatatttta gaaacgttat
     1321 tatttgcaga tatgataaca gaagaagatg tacaaaattt cgaaatagga gaatttttag
     1381 cacccgataa acaagacgtt ttgacaacgt tgcattcatt ctatagtatc ggcgccttga
     1441 aacagatatt taagcaatca tttaaacgaa agcaattagg attctttcgc atgattggct
     1501 attgcaaatt ggatcaatgt agacggaagt atttattaga atttttcggt gaatatccac
     1561 cggcacaaga tcgatgttgt gacaatgatt ctaatataac tgatatcgta attttaaata
     1621 agaagaaggt aattagaagt attggatttg atgaaaagtt gcaaaattta tttctcagat
     1681 agtattactt tactaaaaga aaattgacaa gctataatta gtgtatacac aattgaaaaa
     1741 tgattgaaat aattttgaaa aatatacata aacatatgtc atgtgggtat attttatgta
     1801 aaatcattgt aatagaatag aaaggaagat ggctatgtct aataatttta aagatgactt
     1861 tgaaaaaaat cgtcaatcga tagacacaaa ttcacatcaa gaccatacgg aagatgttga
     1921 aaaagaccaa tcagaattag aacatcagga tacaatagag aatacggagc aacagtttcc
     1981 gccaagaaat gcccaaagaa gaaaaagacg ccgtgattta gcaacgaatc ataataaaca
     2041 agttcacaat gaatcacaaa catctgaaga caatgttcaa aatgaggctg gcacaataga
     2101 tgatcgtcaa gtcgaatcat cacacagtac tgaaagtcaa gaacctagcc atcaagacag
     2161 tacacctcaa catgaagagg aatattataa taagaatgct tttgcaatgg ataaatcaca
     2221 tccagaacca atcgaagaca atgataaaca cgagactatt aaagatgcag aaaataacac
     2281 tgagcattca acagtttctg ataagagtaa agctgaacaa tctcagcaac ctaaaccata
     2341 ttttgcaaca ggtgctaacc aagcaaatac atcaaaagat aaacatgatg atgtaactgt
     2401 taagcaagac aaagatgaat ctaaagatca tcatagtggt aaaaaaggcg cagcaattgg
     2461 tgctggaaca gcgggtgttg caggtgcagc tggtgcaatg ggtgtttcta aagctaagaa
     2521 acattcaaat gacgctcaaa acaaaagtaa ttctgacaag tcgaataact cgactgagga
     2581 taaagcgtct caagataagt ctaaagatca tcataatggc aaaaaaggtg cagcgatcgg
     2641 tgctggaaca gcaggtttgg ctggaggcgc agcaagtaaa agtgcttctg ccgcttcaaa
     2701 accacatgcc tctaataatg caagccaaaa ccatgatgaa catgacaatc atgacagaga
     2761 taaagaacgt aaaaaaggtg gcatggccaa agtattgtta ccattaattg cagctgtact
     2821 aattatcggt gcattagcga tatttggagg catggcatta aacaatcata ataatggtac
     2881 aaaagaaaat aaaatcgcga atacaaataa aaataatgct gatgaaagta aagacaaaga
     2941 cacatctaaa gacgcttcta aagataaatc aaaatctaca gacagtgata aatcaaaaga
     3001 ggatcaagac aaagcgacta aagatgaatc tgataatgat caaaacaacg ctaatcaagc
     3061 gaacaatcaa gcacaaaata atcaaaatca acaacaagct aatcaaaatc aacaacagca
     3121 acaacaacgt caaggtggtg gccaaagaca tacagtgaat ggtcaagaaa acttataccg
     3181 tatcgcaatt caatactacg gttcaggttc accggaaaat gttgaaaaaa ttagacgtgc
     3241 caatggttta agtggtaaca atattagaaa cggtcaacaa atcgttattc cataatataa
     3301 ctatataaat tgtaactgaa ctgctatata caaacgtgca tattataagt atctctaaat
     3361 attttggaga acttaaattg cgcttataag tatgtagcgg ttttttcatt tttcaaagtt
     3421 tgttatttaa caaggtcttg tctcgaatat tggcatatca attaaacttt ttaaatagtc
     3481 atcaaaaaga taaaacacca caatcaacaa atttaacgag ggagaataaa aaataaatca
     3541 acatattaaa ttgtagtgtt attcaactcc gtagctaaca attctctatg cacattaaac
     3601 aaattgtcaa aaatatatca taaatcttca agcacagact tagcgcatca atcactgaac
     3661 tgttataata gtttgggatt aaaggaggcc gaaacaatgc aaaaagttga aagtatcata
     3721 attggtggag ggccatgcgg attaagtgcg gctattgaac aaaaaagaaa aggtattgat
     3781 accttaatta ttgaaaaggg gaatgtcgtt gaatcaatct ataattatcc tactcaccaa
     3841 acatttttct catcaagtga taaattaagt attggggacg taccgtttat cgttgaagaa
     3901 agtaaaccaa gacgtaatca agcgctagtt tattaccgag aagttgtaaa acatcatcaa
     3961 ttaaaagtaa atgcatttga agaagtatta actgttaaaa aaatgaataa taaatttact
     4021 attactacga cgaaagatgt ttatgaatgt cgatttttaa caatcgcgac aggctattat
     4081 ggtcagcata atacattaga agttgaagga gcggatttac ctaaagtgtt ccattatttt
     4141 aaagaggcac atccgtattt tgatcaagat gttgttatta ttggtggtaa gaattcggct
     4201 atcgatgctg ctttggagtt ggaaaaagct ggtgctaacg tgacggttct atatcgtggt
     4261 ggagattatt cgccttcaat taaaccgtgg atacttccaa atttcacagc attagtaaat
     4321 catgaaaaaa ttgacatgga atttaatgct aatgttaccc aaataactga agatactgtg
     4381 acttatgaag taaatggtga aagtaaaaca atacacaatg attatgtatt tgcgatgatt
     4441 ggttatcatc ccgattatga atttttaaaa tctgtaggca ttcaaattaa tacaaatgaa
     4501 tttggaacag cgcctatgta taataaagaa acatacgaaa caaatatcga aaattgctat
     4561 attgcaggtg taattgctgc agggaacgat gcgaatacca tttttattga aaatggtaaa
     4621 ttccacggtg gcattattgc tcaaaacatg ctagctaaga aacaaacgcc gttagaatca
     4681 taaaaataaa ggtctatgta ctaaataact tagttttaca acgactgaca ttcatgatat
     4741 gtcaaatgag gttgatgact aattattgta catagacctt tttatgttac gtattcatta
     4801 taattcaaaa tatgatttga tttcagcttt atctaaattg ttgcttaacg caactaataa
     4861 ttttaatctt gcttttggac cattcgaacc gttagaaaaa ataaaacctt gttgtgcgag
     4921 ttggtaacca ccaccatcgt atgcgtaagt tggactcaca ataccattaa aggaacgtga
     4981 aactagcaca ataggtatat ttaaagatac taattgttga atgccttcta atgcgcttgg
     5041 aggtatgttg ccttgtccta acgcttcaat aaccatacca tcgatacctt cacgactata
     5101 aaaactaaaa atgtcacctg gcatacccat atatgctttt actaatggta catttagttt
     5161 gtcattgaca ttttccaatg cttgttggcg atatggcata tgatggaatt gcacacgatc
     5221 ctttgtcaat acacctagcg gaccatgatt tggactttga aatgtgtttg tattagacgt
     5281 atgtgttttg gtaacattac gcgccgtatg aatttcatca ttaaatacaa ccatcacgcc
     5341 tttatgacgg gccttttcat cagaggcaac gcgaatagcg gaaatataat tatataatcc
     5401 gtcagaacca atttcattag acgagcgcat tgcgccagta ataacaacag gttgctcgat
     5461 acctaatatt aaatcaagta aaaaggcagt ttcttctaac gtatcggtac catgcgtgat
     5521 aacgaaacca tcataatatt tatttgttac tgcttctaaa ataatatcct ttaattgttt
     5581 aacatgttgg attgtcatat gaggtgatgg tacattaaaa ggatttaatt catctatttg
     5641 tgcatattga tttatgacat cttgatgcat tgaaataggg ttgatatcat ttgttactac
     5701 tttattagat tggtcttgtg acatactaat ggtgccacca gtatgaataa caagtagatg
     5761 tttcatatat ttcctcctat atttaattta cctaattatg ataaaatatt attcataaaa
     5821 cgacaaggaa gggaaatgac gcatgaaagc cattaatatt gcattagatg gtccagctgc
     5881 tgccggaaaa agtacaattg cgaaacgtgt agccagcgaa ctatcaatga tttatgtcga
     5941 tacaggagca atgtatcgtg cattaacata caaatattta aaattaaaca aaactgagga
     6001 ctttgcaaaa ctagttgacc aaacaacatt agatttaact tataaagcag ataaaggtca
     6061 atgtgtcatt ttagataacg aagatgtaac agacttttta agaaataatg atgtgacgca
     6121 acatgtttca tacgttgcat ctaaagagcc agtacgttca ttcgccgtta aaaaacaaaa
     6181 agagttagct gcagaaaaag gtatcgtaat ggatggtcgc gatatcggaa ctgtagtgct
     6241 accagatgca gatttaaaag tatatatgat tgcatcagtt gaagagcgag cagaaagaag
     6301 atataaagat aatcaattaa gaggtatcga atcaaatttt gaagatttaa aacgtgatat
     6361 tgaagctcgt gatcaatatg acatgaaccg tgaaatatca ccattaagaa aagcagatga
     6421 tgcagtgaca ttagatacga caggcaagtc gattgaagaa gttactgacg aaattttagc
     6481 gatggtgagt caaattaaat aatctaataa gtcataacta tctatttgta atgtgcaacg
     6541 cttaacgcat atacaaaatg aatgtgctga taatgattta ctcaaattaa aaggtgattt
     6601 ttattcaatg atgaatgaaa gttgcctttt tatttttggt aaaagttcat gcgtcagtga
     6661 attgtgtaag
//
LOCUS       DAHHSC010000132         6662 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.133, whole genome shotgun sequence.
ACCESSION   DAHHSC010000132 DAHHSC010000000
VERSION     DAHHSC010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 6662)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6662)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6662
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.133"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            13..1503
                     /locus_tag="NBI57_001924"
     CDS             13..1503
                     /locus_tag="NBI57_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HCD1822859.1"
                     /translation="MNYFVGNSLGVNLTGIEKAIINRLNLFKEMGRPAQCVFLSWNRY
                     LYRNAQNYITSSDYINMYDFFQEATYLERNEPFDWLSYWTDECHYTLKHVENSHDFRI
                     YDQERFLMYAHFQDPKYRILDYVNHFDSQRRKVKRDFYDVRGFLSCSRILVDKQQTLC
                     EFFYNPEGDTKLEKYFSYKDGKPEVQKIIVYYANKQYFFNNETELGAFFIKQLYQHGD
                     LFFSDRNVYTAPIFNLTPESIPVVAVLHSTHIKNIDALDSSPFKNVYKAMFENLSRYR
                     AIIVSTEQQKLDVEKRINHTIPIVNIPVGYSETIDTPVQTLDQRSVKLISVARYSPEK
                     QLHQQIELIKRLVSYVPKIELHMYGFGSESKKLNELIQKYGLENHVYLRGFLSNLNQE
                     YSDAYLSLITSNMEGFSLALLESLAHGVPVISYDIKYGPNELITPDFNGYLITKNDED
                     ALFDKVKYVIDHPEVQQRLSKGSLAKAQQYSKASLIKQWDQFVRLI"
     gene            complement(1628..3100)
                     /locus_tag="NBI57_001925"
     CDS             complement(1628..3100)
                     /locus_tag="NBI57_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HCD1822860.1"
                     /translation="MNYILGTILESKITGVEKAQINRLKLFKQHGISSKCVYVKWNPY
                     SYTYAKQHQIENDVFTMYDYFQKAINYKKTKQVNWIQYWEKSCRYTLKFVENSNDVRI
                     YDEEQFVMYAHFLDKQYHQLNYVNYFDHKRRKVKRELYDGRGFLSCSRILGEGQRIVL
                     ENYYTPNGEIVIQKYFDDIKGKNTLTKVILNEDQQQQFFDTEDELVQYFLHQLCKNND
                     QIILDRPHELGNVIAGLNQSIPVVVVLHSTHLSGTGNGIKSFYKTVFNNLTRYKAIVV
                     STEQQCQDISQYIENKIPVINIPVGYVANLKYQFDINQKEKNHIISIARLVENKQIKH
                     QIEVIKQLVTKHPNIQLNIYGHGNGLSEYRQLVEDYHLSEHVKFHGFKTHINEEIAKA
                     ELMLSTSKMEGFGLAILESLSVGTPVISYDIDYGPSELIQDGFNGYLVPKGDINQMVE
                     KVDQLLNNTQKLQQFSINSIESAQQYNATTISTKWQNILN"
     gene            complement(3221..6634)
                     /gene="sdrE"
                     /locus_tag="NBI57_001926"
     CDS             complement(3221..6634)
                     /gene="sdrE"
                     /locus_tag="NBI57_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_046462978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MSCRAMM family adhesin SdrE"
                     /protein_id="HCD1822861.1"
                     /translation="MINRDNKKAITKKGMISNRLNKFSIRKYTVGTASILVGTTLIFG
                     LGNQEAKAAENTSTENAKQDDATTSDNKEVVSETENNSTTENDSTNPIKKETNTDSQP
                     EAKEESTTSSTQQQQNNVTATTETKPQNIEKENVKPSTDKTATEDTSVILEEKKAPNY
                     TNNDVTTKPSTSEIQTKPTTPQESTNIENSQPQPTPSKVDNQVTDATNPKEPVNVSKE
                     ELKNNPEKLKELVRNDNNTDRSTKPVATAPTSVAPKRLNAKMRFAVAQPAAVASNNVN
                     DLITVTKQTIKVGDGKDNVAAAHDGKDIEYDTEFTIDNKVKKGDTMTINYDKNVIPSD
                     LTDKNDPIDITDPSGEVIAKGTFDKATKQITYTFTDYVDKYEDIKARLTLYSYIDKQA
                     VPNETSLNLTFATAGKETSQNVSVDYQDPMVHGDSNIQSIFTKLDENKQTIEQQIYVN
                     PLKKTATNTKVDIAGSQVDDYGNIKLGNGSTIIDQNTEIKVYKVNPNQQLPQSNRIYD
                     FSQYEDVTSQFDNKKSFSNNVATLDFGDINSAYIIKVVSKYTPTSDGELDIAQGTSMR
                     TTDKYGYYNYAGYSNFIVTSNDTGGGDGTVKPEEKLYKIGDYVWEDVDKDGVQGTDSK
                     EKPMANVLVTLTYPDGTTKSVRTDANGHYEFGGLKDGETYTVKFETPAGYLPTKVNGT
                     TDGEKDSNGSSITVKINGKDDMSLDTGFYKEPKYNLGDYVWEDTNKDGIQDANEPGIK
                     DVKVTLKDSTGKVIGTTTTDASGKYKFTDLDNGNYTVEFETPAGYTPTVKNTTAEDKD
                     SNGLTTTGVIKDADNMTLDSGFYKTPKYSLGDYVWYDSNKDGKQDSTEKGIKDVKVTL
                     LNEKGEVIGTTKTDENGKYRFDNLDSGKYKVIFEKPAGLTQTVTNTTEDDKDADGGEV
                     DVTITDHDDFTLDNGYFEEDTSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDS
                     DSDSDSDTDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDS
                     DSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDAGKHTPVKPMS
                     TTKDHHNKAKALPETGSENNGSNNATLFGGLFAALGSLLLFGRRKKQNK"
BASE COUNT         1926 a         1118 c         1037 g         2581 t
ORIGIN      
        1 gtggtgttgt aaatgaatta ttttgtaggt aatagtttag gtgttaattt aactgggata
       61 gaaaaagcaa ttattaatcg tttaaattta tttaaagaaa tgggaagacc agcgcaatgt
      121 gtatttctgt catggaatag atatttatat agaaatgccc aaaattatat tactagttct
      181 gactatatta atatgtatga tttcttccaa gaagcaacat atttagaacg caatgaacca
      241 tttgactggt tgtcgtattg gactgatgaa tgtcattata ctttaaaaca tgttgaaaat
      301 agtcatgact ttagaatata tgatcaagag cgtttcttaa tgtatgccca ttttcaagat
      361 cctaaatatc gtatattaga ttatgtgaat cattttgata gtcaaagacg aaaagtaaaa
      421 cgtgattttt atgatgtgcg tggcttttta agttgtagta ggattcttgt tgataaacaa
      481 caaacattat gtgaattttt ctataatcca gaaggtgaca caaaattaga aaagtatttt
      541 tcttacaagg atggcaaacc agaagtacaa aaaattattg tttactatgc taacaaacaa
      601 tatttcttta ataatgaaac ggaattaggc gcatttttca ttaaacaact atatcaacat
      661 ggggatttat ttttcagtga ccgtaatgtg tatacagcac ctattttcaa cttaacacct
      721 gagtcaatcc cagtagtagc tgtgcttcac agtacacaca ttaaaaatat tgatgcatta
      781 gattcatcgc catttaaaaa tgtatataaa gcgatgtttg aaaatttatc tcgatatcgt
      841 gcaattatcg tttctactga gcagcaaaaa ttagatgttg aaaaacgtat taaccatact
      901 attccaattg ttaatattcc tgtcggctat agtgaaacaa ttgatacacc agtccaaaca
      961 ttagatcagc gcagtgttaa attaatttcc gttgctcgtt attcacctga aaaacaatta
     1021 catcaacaaa ttgaattaat taaacgctta gtatcgtatg tcccaaaaat agagcttcat
     1081 atgtatggtt ttggttctga aagtaaaaaa ttaaatgaat tgatacaaaa atatggattg
     1141 gaaaatcatg tttacttacg tggcttttta agcaatctaa accaagaata cagcgatgct
     1201 tacttaagtt taataacaag taacatggaa ggtttctctt tagcgctact tgaatcttta
     1261 gcacatggtg ttccagttat tagttatgat attaaatatg gacctaacga attgattacg
     1321 cctgatttca atggttattt aattactaaa aatgatgaag atgccctatt tgataaagta
     1381 aaatatgtga ttgatcatcc tgaagtacaa caacgactgt caaaaggtag tttggcaaaa
     1441 gcgcaacaat atagtaaagc gtcgttaatt aaacaatggg accaatttgt tcgtcttata
     1501 taaatgtact tcacctttat ttttataaaa aaaagcgttt tcctatgtca tttaactaaa
     1561 catgtaagtt cgtatgaacg ttgtttgtta aatagatgat tctaggaaaa tgcttttttc
     1621 ttttgactta gtttaaaata ttttgccact ttgtactgat agtagttgca ttgtactgtt
     1681 gtgcagattc tatgctatta atcgaaaact gttgcaattt ttgagtatta tttagtaatt
     1741 gatcgacctt ttcaaccatt tgattgatgt cacctttagg tactaaatag ccattaaatc
     1801 catcttgaat cagttctgat ggcccataat ctatatcata actgatcact ggtgtaccta
     1861 ctgaaagcga ctctaaaatt gctaagccaa aaccttccat tttacttgtc gataacatca
     1921 gttctgcttt agcaatctct tcattaatat gcgtcttaaa accatgaaat ttaacatgtt
     1981 ccgataaatg ataatcttct acaagttgtc gatattctga caaaccattt ccatgtccat
     2041 aaatattcaa ttgaatattg ggatgttttg ttactaattg cttgatcact tcaatttgat
     2101 gtttaatttg tttattttca acgaggcgag caattgatat gatatgattt ttctcctttt
     2161 gattgatgtc aaattgatac tttaaatttg ccacgtagcc aaccggaata ttgataactg
     2221 gtattttatt ttcaatatat tgtgaaatat cttggcattg ctgttctgtt gatacaacaa
     2281 tcgctttata acgtgttaaa ttattaaata ctgttttata aaaacttttt ataccattac
     2341 cggtaccgga taaatgtgta ctatggagca caacaacaac tggaatactt tgatttaatc
     2401 ccgctataac atttcctaat tcatgaggac gatctaatat gatttgatca ttatttttac
     2461 ataattgatg gagaaaatat tgaactaatt catcttctgt atcaaaaaat tgttgctgct
     2521 ggtcttcatt taagataacc tttgtgagcg tgttttttcc ttttatatcg tcgaaatatt
     2581 tttggatgac gatttcccca ttaggtgtat agtaattttc gagtacaatc cgttgtcctt
     2641 cacctaaaat tcgagaacaa cttaaaaagc ctcttccatc atacaattcg cgttttactt
     2701 ttcttctttt atgatcaaaa taattcacat agtttaattg atgatactgt ttatctaaaa
     2761 aatgagcata cattacaaat tgctcttcat catatattct gacatcattt gaattttcca
     2821 caaatttcaa tgtgtaccta catgactttt cccaatactg tatccagtta acttgctttg
     2881 tctttttata attgattgct ttttgaaaat agtcatacat tgtaaataca tcattttcaa
     2941 tctgatgttg cttcgcatat gtgtatgaat aaggattcca tttaacatat acacattttg
     3001 aagatatgcc gtgttgtttg aacaacttca atctatttat ttgcgctttt tctacacctg
     3061 taattttact ttctaaaatt gttcctaaaa tgtaattcat attatcgcct catataagtt
     3121 ttattccgta tctttattgt ttattttata tgaaaaatac atctattgca tgtgtaatca
     3181 taaaaaaaaa ccaggccaca aggacctggg tcatattgta ttatttgttt tgttttttgc
     3241 gacgaccgaa taacaataat gaacctaatg ctgcaaataa tccaccaaat aacgttgcgt
     3301 tatttgagcc gttattttca ctacctgttt ctggtaatgc ttttgcttta ttgtgatggt
     3361 ctttagtagt actcattggt ttaacaggtg tatgttttcc tgcatccgag tccgaatcgc
     3421 tatctgagtc tgaatcactg tctgagtctg agtcgctatc tgagtctgag tcgctgtccg
     3481 aatctgagtc gctatctgag tctgaatcac tgtccgaatc tgagtcgcta tctgaatctg
     3541 aatcgctgtc tgagtctgag tcactgtctg agtctgagtc gctatctgag tctgagtcac
     3601 tgtctgaatc tgagtcgcta tctgagtctg aatcgctgtc tgaatctgag tcgctatctg
     3661 agtctgaatc gctgtccgaa tctgagtcgc tatctgtgtc tgagtctgag tcgctatctg
     3721 agtctgagtc gctgtctgaa tctgaatcgc tatctgagtc tgaatcactg tctgaatctg
     3781 agtcgctgtc tgagtctgaa tcactgtctg agtctgaatc actgtctgat gtatcttctt
     3841 cgaagtatcc gttatcaagt gtgaaatcat catgatccgt aattgttacg tcaacttcgc
     3901 caccatcggc atctttatca tcttcagttg tatttgtaac tgtttgtgtt aagccagcag
     3961 gcttttcaaa aataactttg tatttaccgc tatctaaatt atcgaaacga tatttaccat
     4021 tttcatctgt tttagttgtt ccaattactt cgcctttttc atttaataaa gtaactttaa
     4081 catctttgat acctttttca gttgaatctt gtttaccgtc tttattactg tcgtaccaaa
     4141 cataatcacc taaactgtat tttggtgttt tatagaaacc actgtctaat gtcatattat
     4201 ctgcatcttt aatgacacct gttgttgtta aaccattaga atctttatct tcagctgtag
     4261 tatttttaac cgttggcgtg taacctgctg gtgtttcaaa ttctactgta tagttaccat
     4321 tatctaaatc tgtaaattta tatttacccg aggcatcagt agtagttgta ccaataactt
     4381 ttccagtact atcttttaat gtaaccttaa catctttgat accaggttca ttagcatctt
     4441 ggataccatc tttatttgta tcttcccata catagtcacc aagattatat ttaggttctt
     4501 tataaaaacc agtgtctaaa gacatatcat ctttaccatt aattttaaca gttatagaac
     4561 taccatttga gtctttttca ccatcagttg ttccatttac ttttgttgga agatatccag
     4621 ctggcgtttc gaatttaact gtataagttt ctccgtcttt caaaccaccg aattcataat
     4681 gaccgttagc atctgttctt actgattttg tagtaccgtc cgggtaagtt aatgtaacta
     4741 aaacgtttgc cattggcttt tctttcgaat ctgtaccttg gacaccgtct ttatcaacgt
     4801 cttcccatac atagtcacca attttgtata acttttcttc aggtttaaca gtaccgtcgc
     4861 caccgccagt gtcattagaa gttacgatga agtttgaata tcctgcataa ttataataac
     4921 catatttatc agttgttctc atactagtac cttgagcaat atctagttcg ccatctgatg
     4981 taggtgtata tttactaaca actttgataa tataggctga attaatatca ccaaaatcca
     5041 atgttgctac attattacta aatgattttt tattatcaaa ttgacttgtt acatcttcgt
     5101 attgactaaa atcatagatt ctattacttt gaggcaattg ttgattaggg ttaactttat
     5161 aaacttttat ttctgtattt tggtcaataa tggtactacc atttcctagt ttaatatttc
     5221 cataatcatc tacttgacta ccagctatat caactttagt gttagttgct gtttttttca
     5281 aaggattaac ataaatttgt tgttcaatag tttgtttgtt ttcatctaac tttgtaaaga
     5341 tagattgaat gtttgaatca ccatgaacca ttgggtcttg ataatcaaca gaaacgtttt
     5401 ggctagtttc tttacctgct gttgcaaacg ttaaattcaa actagtttca ttaggtactg
     5461 cttgcttatc aatatatgag tataaagtta aacgtgcttt tatatcttca tatttatcta
     5521 cataatctgt aaatgtatat gtgatttgct tagtcgcttt atcaaatgtt cctttggcaa
     5581 tgacctctcc tgatggatca gtaatatcga taggatcatt tttatctgtt aaatccgaag
     5641 gaattacatt cttatcataa ttaatcgtca ttgtatcgcc ttttttgact ttattgtcaa
     5701 ttgtaaactc tgtatcatat tcaatatctt taccgtcatg cgctgctgcc acattatctt
     5761 taccatcgcc aactttgatc gtctgtttcg taactgtaat taagtcattt acattatttg
     5821 aagcaactgc tgctggttgt gcaactgcaa aacgcatttt cgcatttaat cgttttggtg
     5881 caacacttgt tggagctgta gcaactggtt tagttgaacg atctgtattg ttatcatttc
     5941 taactaattc ttttaatttc tcaggattat ttttaagttc ttcttttgac acatttactg
     6001 gttcttttgg attagttgca tctgtaactt gattgtctac ttttgaaggc gttggttgcg
     6061 gttgtgaatt ttcaatattt gtagattctt gaggtgtagt tggttttgtt tgaatttcac
     6121 ttgtagatgg ttttgtagtt acatcgttat ttgtataatt tggtgctttc ttctcttcta
     6181 aaataacaga tgtatcttct gtcgcagttt tatcagttga aggtttaaca ttttcttttt
     6241 caatgttttg aggcttagtt tcagttgtag ctgtaacgtt attttgctgt tgttgagtac
     6301 ttgatgtagt tgattcttct ttagcttctg gttgtgaatc agtatttgtt tctttcttaa
     6361 ttggatttgt tgaatcattt tctgttgtcg aattattttc agtttccgac actacttctt
     6421 tattatcact agtcgttgca tcatcttgtt tcgcattttc tgtactagtg ttttcagcag
     6481 ctttagcttc ttggttccct agaccaaaaa tcaatgtcgt acctactaaa atcgatgcag
     6541 ttcctacagt atactttcta atcgaaaatt tgtttaagcg atttgaaatc ataccctttt
     6601 ttgttattgc ctttttatta tccctgttaa tcatataaat ctcctttaaa atatcaatat
     6661 tt
//
LOCUS       DAHHSC010000133         6504 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.134, whole genome shotgun sequence.
ACCESSION   DAHHSC010000133 DAHHSC010000000
VERSION     DAHHSC010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 6504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6504
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.134"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            complement(121..813)
                     /locus_tag="NBI57_001927"
     CDS             complement(121..813)
                     /locus_tag="NBI57_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HCD1822862.1"
                     /translation="MNYSPKQGVRSHGLPHDPFKSSTVPRPIGWISTVSKDGKDNLAP
                     YSQYQNLTWDPPMVMFAANQSVLGDHERKDTVKNAEETGWFVWNMATYDLREAVNLSS
                     KALPPEEDEFEYAGVTKEACIEAPGYRVKESPVHFECEYVQTIRIPTGDPVSTVDIVI
                     GRVAQVHIDDKVILDNGKLDIKSIKPIARLGYYDYTVVNEIFEMKAPAASKEELAGLE
                     GRNFDNQSDEKR"
     gene            1049..1507
                     /locus_tag="NBI57_001928"
                     /pseudo
     CDS             1049..1507
                     /locus_tag="NBI57_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001226788.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1494..1796
                     /locus_tag="NBI57_001929"
     CDS             1494..1796
                     /locus_tag="NBI57_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1822863.1"
                     /translation="MRTNNHITDLDAIELSSGWNYNSKLVTGKNALLSDSLLRDIKVL
                     SSNAKGDADILETELRAVINAMSQNEGKSVDALKNSTTSLKNNLTKLKEQLDKTTH"
     gene            1950..2309
                     /locus_tag="NBI57_001930"
     CDS             1950..2309
                     /locus_tag="NBI57_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1822864.1"
                     /translation="MILRYATNLKLAKELYQASKPKLQEDKGMIELFEHTFGLQKELV
                     KYVGIAEATTAALYSASFINKNISRLASLSTIGILSVAAYKHFEAGQGKKGAQHALDL
                     LGLATLSLLDTFSCNKK"
     gene            complement(2394..3005)
                     /locus_tag="NBI57_001931"
     CDS             complement(2394..3005)
                     /locus_tag="NBI57_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HCD1822865.1"
                     /translation="MYTFDAQSLTARENYKLLIGSIIPRPIAFVTTLNQDASVNAAPF
                     SFFNIVNNHPPMVAIAVQRAGGKRKDTALNIERTGDFVVHITDEDNVQDINETAAPLA
                     YGDSELSRTGLSLLTSTTIKTPGIKDAKMRFECKLSQMILLGDVLDGADLIIGEIVTY
                     HIDDSIYEGDFKINPHALQAVSRLAGNDYAKLGGIFTIKRPEK"
     gene            complement(3084..3560)
                     /locus_tag="NBI57_001932"
     CDS             complement(3084..3560)
                     /locus_tag="NBI57_001932"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase"
                     /protein_id="HCD1822866.1"
                     /translation="MVVVYVAGGCLWGVEAFFATIPGIIHTEAGRANGRSSKLDGPYD
                     GYAECVKLHFDDRMLTITDIMNYLFEIIDPYSVNQQGNDIGEKYRTGLYSCVDDHLIE
                     ARQFIERRKDRDQIAVEVLPLSNYIKSAEEHQQHLEKYPEDMHMCHISKDLLNKYK"
     gene            complement(3563..4063)
                     /locus_tag="NBI57_001933"
     CDS             complement(3563..4063)
                     /locus_tag="NBI57_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCD1822867.1"
                     /translation="MVLRKVIMQDLDQIIALENIGFSPEEAATREALKLRIEQIQETF
                     IVAEKNDEVIGYINGPVIKERYISDDLFKNVSINNSEGGYISVLGLVVAPNYQGQGIA
                     GRLLNYFETLAKNHHRHGVTLTCRESLISFYEKYGYRNEGVSESCHGGIKWYNLVKNI
                     KKDDEV"
     gene            complement(4298..5065)
                     /locus_tag="NBI57_001934"
     CDS             complement(4298..5065)
                     /locus_tag="NBI57_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein phosphatase"
                     /protein_id="HCD1822868.1"
                     /translation="MIDIHNHILVDIDDGPKTIEKSIALLKQAKDEGVTSIVATPHHL
                     HPRYDNTFQQVLVKLAELRTHPEVQALDIKLFPGQEIRITDSILKGLDNGSIQGINRS
                     KYLLIEFPTGEVPHYTKQLFFEIQSRGYIPIIAHPERNRSIAKNPEILYELVANGALS
                     QLTSSSLVGGFGKNIQKLSLQFIECNLAHFVASDAHSCDQRPFLMQELFHNHKLKKYS
                     NDIEALLRNASSVINDNFVYLDRPTKPGKVKSFLKWF"
     gene            complement(5062..5754)
                     /locus_tag="NBI57_001935"
     CDS             complement(5062..5754)
                     /locus_tag="NBI57_001935"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis tyrosine autokinase"
                     /protein_id="HCD1822869.1"
                     /translation="MTNTRRSTSSLIVHEQPKSPISEKFRGIRSNIMFANPDSAVQSI
                     VITSEAPGAGKSTIAANLAVAYAQAGYKTLIVDGDMRKPTQHYIFNLPNNEGLSSLLL
                     NWSTYQDSIISTEIQDLDVLTSGPIPPNPSELITSRAFANLYDTLLMNYNFVIIDTPP
                     VNTVTDAQLFSKFTGNVVYVVNSENNNKDEVKKGKELIEATGAKLLGVVLNRMPKDKS
                     ASYYAYYGTDES"
     gene            complement(5771..6433)
                     /locus_tag="NBI57_001936"
     CDS             complement(5771..6433)
                     /locus_tag="NBI57_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide biosynthesis protein
                     CapA"
                     /protein_id="HCD1822870.1"
                     /translation="MKEKFDLVKLLNILKKNIKLLLILPAICLVVSTALTFFVMPDKY
                     TASTQILVNMKKSSSDLAFQNVQSSLQSVNTYTEIIKSPRILDKVSREFDGQYSTAEL
                     NSFLKVTNQTNSQIITVSVTTGNKSESDKIVNRISKVFAHDMPKIMSVDNVTILSSAH
                     DNAVKVSPIVSVNLVISIIVGIVLAILIIFLKELLDKRIKTEEDVESQLGLPILGSIQ
                     KF"
BASE COUNT         2133 a         1193 c          928 g         2250 t
ORIGIN      
        1 aaaatataaa taccacgaca tattgcttta acattttcat ttataaagcg aaaaaatgca
       61 tcgctactaa gttgaatatt tagtaacaat gcattgaatt cactaaaatg attaaattac
      121 ttatctcttt tcatctgatt gattatcgaa atttcttcct tctaaacctg ctaactcttc
      181 tttagaagct gcaggtgctt tcatttcaaa tatctcattc actactgtgt aatcgtaata
      241 tcctaatctg gcaataggtt taatcgactt aatgtccaat ttaccattat caagaataac
      301 cttatcgtca atatgaactt gggcaactct tcctataaca atatctacgg tagatactgg
      361 gtctcctgtc ggaatacgaa tcgtttgaac gtactcacat tcaaaatgta ctggcgattc
      421 tttgactcga taacctggtg cttctataca tgcttctttc gtcacacctg catattcaaa
      481 ttcatcctct tcaggtggta gtgccttcga cgataaatta acagcttctc ttaaatcata
      541 cgtggccatg ttccatacaa accaccctgt ctcttctgca ttttttactg tatctttacg
      601 ctcatgatca ccaagaacag attgatttgc tgcgaacata accataggcg gatcccaagt
      661 taagttttga tactgactat aaggcgctaa attatctttc ccatctttcg atacagtaga
      721 aatccaacct attggacgag gtactgtact acttttaaat gggtcgtgcg gtaaaccatg
      781 acttcttaca ccttgttttg gcgaataatt catactatct tcacccctta taagtaatta
      841 catttaaggt tacgccctct ttacataagc gtctaatata aataaacaat ttatttataa
      901 gtagaaacta tatatgacgt ggttgcttat aatttgcgtt cttgattcga aaaattcaga
      961 taaggattta tacaattaat atttatgata tcttttgtaa atttaattaa ttatagttac
     1021 ttcaatcatg attagtttat aataataaag tgaaattgaa aaagacagct attatgcgat
     1081 gagcgaaaaa cttcaagtaa aacaagaatt taacggcgtt acaaggcaat acttgaattt
     1141 cagaacagat agtatcgatg atctggataa gttcaaaaaa gaattcaaac ctgttatgca
     1201 cttaaaaggt gatgcattca atcaacagtt acaatctttg attaacaaat atccacaaat
     1261 acaaaaaaat atgaaatcag agttcattgc ttattatgat aaagaaaaaa atagagaaac
     1321 agtaaaaaac tatgcttgga accttcaaaa atctataaat gacattatgc aatcatatcc
     1381 tagcacaaaa tttgtacagt tttataaaga tgatgtttcc ccgtcaatgg tagatggaaa
     1441 tggccgttta aaatcggata ctaatgtaat ttccatcgag ggaggaaagt atgatgagaa
     1501 caaataatca tataaccgat ttagatgcta ttgagttaag tagcggttgg aattacaatt
     1561 cgaaattggt gactggcaaa aatgcactac tatcagatag tcttttaaga gatattaaag
     1621 tattaagttc taatgctaaa ggcgacgctg acatactaga aacagaactt cgtgctgtaa
     1681 taaacgcaat gtctcaaaac gaaggtaaat ctgttgatgc acttaaaaat agtaccactt
     1741 ctctaaaaaa caatctaacc aaattaaaag agcaactcga caaaactaca cattaatttc
     1801 atacattttg agtgaacaga attatatttc catagcaaac attcctaaac tacacatacg
     1861 ttacataatt gattcatttt tatagaaacg ggtaaaaatg ataaagtaat catctttcac
     1921 aaaaatccaa agattgggag gaattaaata tgattttgcg ttacgcaaca aatttaaaac
     1981 ttgcgaaaga gctataccaa gccagtaaac ctaaattgca agaagataag ggtatgattg
     2041 aactgtttga acatactttt ggcttgcaaa aagaacttgt aaaatacgtc ggcattgcag
     2101 aagcaactac tgctgcttta tactcagcta gctttatcaa taaaaacatt tctcgccttg
     2161 catcactatc aacgataggc attttaagtg tagctgctta caaacatttc gaagctggcc
     2221 aaggtaaaaa aggtgcacaa catgcattag atttattagg tcttgctact ttaagtttac
     2281 tagatacatt ttcttgtaat aaaaaatagt cttcatttta gaagcacata ctttagcacc
     2341 tgtgtacttt tgattcaatc cataaacggt acaccgctat agcatgtgtt ttattatttt
     2401 tctggtctct ttatcgtaaa tattccccct aatttagcat aatcatttcc ggctaaacga
     2461 gaaacagcct gtaatgcgtg tggattgatt ttaaaatcac cctcataaat actgtcatca
     2521 atatgataag ttacaatttc acctattatt aaatcagccc catctaatac atctccaagc
     2581 aatatcattt gcgatagttt acattcgaat ctcattttcg cgtctttaat tcctggcgtc
     2641 ttaatcgttg tagatgttaa aagtgataat cctgtacgac tcaactcact gtcaccatat
     2701 gctaacggcg ctgcagtctc attaatatct tgaacattat cttcgtctgt aatatgcaca
     2761 acaaagtctc cagtccgttc tatattcaag gcagtatctt ttctcttacc tcctgcacgt
     2821 tgaactgcaa tagcaaccat tggcggatgg ttattaacaa tattaaaaaa gctaaatggt
     2881 gctgcattta ctgatgcatc ttgatttaat gttgtaacaa atgctatagg tcgtggaata
     2941 attgaaccaa ttaataattt atagttttct ctagcagtta atgattgtgc atcaaacgta
     3001 tacataatac ctacctcttt tcttagtata tctaggtatt tctccgattt tggttaattt
     3061 aaacatctat tctcctctga aaatcacttg tatttattta gcaaatcttt tgaaatatga
     3121 cacatatgca tatcttctgg atatttttct aaatgttgct gatgttcttc agcactttta
     3181 atgtagttag acagcggtaa gacttccact gcaatttgat ctctgtcttt acgtcgttca
     3241 atgaactgac gcgcttcaat taagtggtca tctacacaac tatataaacc cgttcgatac
     3301 ttttctccaa tatcatttcc ttgttgattc acactgtaag gatcaatgat ttcaaataaa
     3361 taattcataa tgtctgtaat tgttaacata cgatcatcga aatgaagttt gacacattca
     3421 gcataaccat catacggacc gtctaattta gagcttcttc catttgctct tcctgcttct
     3481 gtatgtataa ttccaggtat tgttgcaaaa aatgcttcaa caccccatag acaccctcct
     3541 gctacataaa caactaccat atttacacct catcatcctt ttttatattt ttaacaaggt
     3601 tataccattt aataccgcca tgacatgatt ctgatacacc ttcattacga tacccatatt
     3661 tttcataaaa tgaaattaat gattctcgac atgttaacgt tacaccatgt cgatgatgat
     3721 tcttagcaag agtttcaaaa tagtttagta agcgacctgc aataccctga ccttgataat
     3781 ttggtgctac aacaagacct aacacactaa tatagccacc ttcactatta tttatggaga
     3841 catttttaaa taaatcatcg ctaatgtaac gctcttttat gactggaccg ttgatgtatc
     3901 caattacttc atcattcttt tcagctacaa taaaagtttc ttgaatttgt tcaattctca
     3961 atttcaatgc ctctcgtgtt gcagcttctt ccggtgaaaa accaatattt tcaagtgcaa
     4021 taatttgatc taagtcctgc atgattactt ttcttaatac cataaaaaca cttccttagc
     4081 cctatatatc aaaagtaata ccttcatctt tatattcatt attttcaaac taccttcact
     4141 ataaaaaacg agtcttttta ttgaaagcca ttcgccttaa tcaattagac aagttgtatt
     4201 atacgaatta gtaaataatc atgattataa ttcattttaa gcatactcaa aaagactggt
     4261 acatgtatac cagtctaatt cgaaaaaata tatttagtta aaaccatttc aaaaacgact
     4321 tcacttttcc tggcttagtc ggtctgtcta aataaacaaa attatcattg attactgaac
     4381 tggcatttcg aagtaatgct tcaatatcat tcgaatattt cttcaattta tgattgtgaa
     4441 ataattcttg catcaaaaat ggtctttggt cacatgaatg tgcatctgaa gctacaaaat
     4501 gagccaaatt acattctata aattgtaatg ataacttttg aatgttttta ccaaatccac
     4561 caactaaaga actcgatgtt aattgactca atgccccatt tgcaaccaat tcatataata
     4621 tttctggatt tttggcgata cttctatttc tttcaggatg tgcaatgatt ggtatgtagc
     4681 ctctcgattg tatttcaaaa aacaattgtt ttgtataatg tggtacttcg cccgttggaa
     4741 attcaattaa taaatatttc gaacgattaa taccttgaat actaccatta tctaagcctt
     4801 tcagaatcga atctgtaatt ctaatttctt gcccgggaaa taatttaata tccaatgctt
     4861 gaacttctgg atgcgttctt aactccgcca atttcacaag cacttgttga aatgtattat
     4921 catatctcgg atgcaaatga tgaggtgtcg ctacaatact tgttacacct tcatccttag
     4981 cttgctttaa tagtgcaata ctcttttcaa ttgttttagg accatcatct atatcaacta
     5041 atatatggtt atgaatatca atcatgattc atcagtccca taatatgcat agtaactagc
     5101 acttttatct ttaggcattc tatttaagac tacacctaat aatttagcac ctgttgcttc
     5161 aataagttct tttccttttt taacttcatc tttattatta ttttccgaat taactacgta
     5221 gacaacattg ccggtaaact ttgaaaataa ttgcgcatct gtaactgtgt tcactggtgg
     5281 cgtatcgata atgacaaagt tataattcat caataatgtg tcatacaaat ttgcaaatgc
     5341 ccttgatgta attaactctg acggattcgg tgggattggc ccagacgtca agacgtctaa
     5401 atcttgaatt tcagttgaga taatactgtc ttgataagtt gaccaattta gcaataaact
     5461 tgataggcct tcattgtttg gcaaattaaa aatataatgc tgcgtaggtt tacgcatatc
     5521 cccgtctacg attagtgttt tataacctgc ttgcgcatat gcaactgcta aatttgctgc
     5581 aattgtagac ttacctgcgc ctggtgcctc tgaagtgatt acaatgcttt gaactgcact
     5641 gtcaggattt gcaaacataa tatttgatct tatgcctcga aatttctcgc taataggtga
     5701 ctttggttgt tcatggacaa ttaaacttga tgtacttctt cgtgtattcg tcatggtaat
     5761 tcctcgtaaa ttaaaatttt tgtattgaac ctaaaatagg taatcctagt tgcgattcaa
     5821 catcttcttc tgtcttaata cgcttatcta ataattcttt taagaaaata atcaatattg
     5881 ctaaaacaat accaacaata atgctgatca ctaagttgac agatactatt ggagatactt
     5941 ttacagcatt atcatgtgct gaggaaagta tcgtaacatt atcaacactc ataattttag
     6001 gcatatcatg tgcaaaaact ttagatattc tattaacaat tttgtcagat tcagatttat
     6061 tcccagttgt aactgataca gtaataatct gagagtttgt ttgattggtt acttttaaaa
     6121 acgaattcaa ctcagctgtt gaatactgac catcaaactc tctagatact ttatctagaa
     6181 ttcttggact tttgataatt tccgtatatg tattaacaga ctgcaaacta ctttgaacat
     6241 tttggaaagc taaatcactt gaggactttt tcatgttcac tagtatttga gtagaagcag
     6301 tatatttgtc aggcataaca aagaaggtta atgccgtact tactacaaga catattgccg
     6361 gtaaaataag caataattta atattcttct ttagaatatt taatagtttt actaaatcaa
     6421 acttttcttt catggtttcc tccacataat caatcattgt attcattatg tatgttttat
     6481 aaatcggaca attatatcta gttt
//
LOCUS       DAHHSC010000134         6490 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.135, whole genome shotgun sequence.
ACCESSION   DAHHSC010000134 DAHHSC010000000
VERSION     DAHHSC010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 6490)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6490)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6490
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.135"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            123..1571
                     /locus_tag="NBI57_001937"
     CDS             123..1571
                     /locus_tag="NBI57_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCD1822871.1"
                     /translation="MGSFFNKIARKEDPAIYQNKDGHLKRTLRVRDFLALGVGTIVST
                     SIFTLPGIVAAEHAGPAVALSFLLAAIVAGLVAFTYAEMAAAMPFAGSAYSWVNVLFG
                     EFFGWVAGWALLAEYFIAVAFVASGFSANLRGLVKPIGIELPAALSNPFGTNGGFIDI
                     IAAIVILLTALLLSRGMSEAARMENILVILKVLAIILFVIVGLTAINVSNYVPFIPEH
                     KVTATGDFGGWQGIYAGVSMIFLAYIGFDSIAANSAEALDPQKTMPRGILGSLSVAIV
                     LFIAVALVLVGMFHYSQYANNAEPVGWALRQSGHGVVAAIVQAISVIGMFTALIGMML
                     AGSRLLYSFGRDGLLPSWLSHLNDKHLPNRALVILTIIGVLIGSMFPFAFLAQLISAG
                     TLVAFMFVSLAMYRLRKREGKDLPIPAFKLPLYPVLPAITFVLVLLVFWGLGFEAKLY
                     TLIWFIVGIILYLSYGLRHSKKNDVAEYHPPK"
     gene            complement(1652..2989)
                     /locus_tag="NBI57_001938"
     CDS             complement(1652..2989)
                     /locus_tag="NBI57_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HCD1822872.1"
                     /translation="MSKAHQLIQEDEHYFAKSGRIKYYPLVIDHGYGATLVDIEGKTY
                     IDLLSSASSQNVGHAPREVTEAIKAQVDKFIHYTPAYMYHEPLVRLAKKLCELAPGDF
                     EKRVTFGLTGSDANDGIIKFARAYTGRPYIISFTNAYHGSTFGSLSMSAISLNMRKHY
                     GPLLNGFYHIPFPDKYRGMYEQPQANSVEEYLAPLKEMFAKYVPADEVACIVIETIQG
                     DGGLLEPVPGYFEALEKICREHGILIAVDDIQQGFGRTGTWSSVSHFNFTPDLITFGK
                     SLAGGMPMSAIVGRKEIMNCLEAPAHLFTTGANPVSCEAALATIQMIEDQSLLQASAE
                     KGEYVRKRMDQWVSKYNSVGDVRGKGLSIGIDIVSDKKLKTRDASAALKICNYCFEHG
                     VVIIAVAGNVLRFQPPLVITYEQLDTALNTIEDALTALEAGNLDQYDISGQGW"
     gene            3247..3663
                     /locus_tag="NBI57_001939"
     CDS             3247..3663
                     /locus_tag="NBI57_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1822873.1"
                     /translation="MRLKVLFHFIAAIFISFMLLWMTMLFDLISNQSHLKALLLNLDF
                     LIPSDNTPYILEIICHLLIGSVIYFVFVLLFHTSKRLYYLCYIPLFFLFIALYPFLVF
                     IAQRPIFQFSVTELIGWIITHIFFMSLMALVIPRIK"
     gene            3786..4676
                     /locus_tag="NBI57_001940"
     CDS             3786..4676
                     /locus_tag="NBI57_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002478556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose bisphosphate aldolase"
                     /protein_id="HCD1822874.1"
                     /translation="MNKEQLEKMKNGKGFIAALDQSGGSTPKALKEYGVNEDQYSNED
                     EMFQLVHDMRTRVVTSPSFSPDKILGAILFEQTMDREVEGKYTADYLADKGVVPFLKV
                     DKGLAEEQNGVQLMKPIDNLDSLLDRANERHIFGTKMRSNILELNEQGIKDVVEQQFE
                     VAKQIIAKGLVPIIEPEVNINAKDKAEIEKVLKAELKKGLDSLNADQLVMLKLTIPTE
                     PNLYKELAEHPNVVRVVVLSGGYSREKANELLKDNDELIASFSRALASDLRADQSKEE
                     FDKALGDAVESIYDASVNKN"
     gene            complement(4870..6366)
                     /gene="mqo"
                     /locus_tag="NBI57_001941"
     CDS             complement(4870..6366)
                     /gene="mqo"
                     /locus_tag="NBI57_001941"
                     /EC_number="1.1.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase (quinone)"
                     /protein_id="HCD1822875.1"
                     /translation="MAKSNSKDIVLIGAGVLSTTFGSMLKEIEPDWNIHVYERLDRPA
                     IESSNERNNAGTGHAALCELNYTVLQPDGSIDIEKAKVINEEFEISKQFWGHLVKSGS
                     IENPREFINPLPHISYVRGKNNVKFLKDRYEAMKAFPMFDNIEYTEDIEVMKKWIPLM
                     MKGREDNPGIMAASKIDEGTDVNFGELTRKMAKSIEAHPNATVQFNHEVVDFEQLSNG
                     QWEVTVKNRLTGEKFKQVTDYVFIGAGGGAIPLLQKTGIPESKHLGGFPISGQFLACT
                     NPQVIEQHDAKVYGKEPPGTPPMTVPHLDTRYIDGQRTLLFGPFANVGPKFLKNGSNL
                     DLFKSVKTYNITTLLAAAVKNLPLIKYSFDQVIMTKEGCMNHLRTFYPEARNEDWQLY
                     TAGKRVQVIKDTPEHGKGFIQFGTEVVNSQDHTVIALLGESPGASTSVSVALEVLERN
                     FPEYKTEWAPKIKKMIPSYGESLIEDEKLMRKIRKQTSKDLELGYYEN"
BASE COUNT         1967 a         1152 c         1109 g         2262 t
ORIGIN      
        1 tacgtaacat aaagttgtga tttttcagaa tttttagaca aaatattgat tatttgtgct
       61 atgatataac atattaaaaa acaccgtata gaattgttta tgaatgtttg ggaggaaagt
      121 ttatgggaag ttttttcaat aaaatagcac gaaaagagga tccggctatc tatcaaaata
      181 aagatggtca tttaaagcgt acacttcggg tgcgtgattt cttagcttta ggtgtaggaa
      241 caattgtatc gacatctatc tttacgctac ctggcattgt tgctgcagaa catgcaggac
      301 cggccgttgc gttatcattc ttactcgctg ctattgttgc tggtttagtt gcatttactt
      361 atgcagaaat ggctgccgct atgccatttg caggttcagc ctattcttgg gtcaatgtat
      421 tatttggtga attttttgga tgggttgccg gttgggctct attagctgaa tattttatcg
      481 ccgtagcctt tgttgcatca ggattctcag cgaatttacg cggacttgtg aaaccaattg
      541 gcatcgaatt acctgcagca ttatcaaatc catttggtac gaatggcggt tttatcgata
      601 ttattgctgc tatcgttatt ttattaactg cattattact atcacgtggt atgtcagaag
      661 cagcacgtat ggaaaacatt ttagttattt taaaagtatt agctattatt ttatttgtca
      721 tcgtaggttt aacagcaata aatgttagta actatgtgcc atttattcca gaacacaaag
      781 taactgctac aggtgacttt ggtggatggc aaggcatata tgctggtgtt tcaatgattt
      841 tcttagcgta tatcggtttc gattctatcg cagcaaactc agcagaagca cttgaccctc
      901 aaaagacaat gcctagaggt attcttggtt ctttaagtgt tgctatcgta ttatttattg
      961 ctgtagcact tgtgttagtt ggtatgttcc attactcaca atacgcaaac aatgctgaac
     1021 ctgttggttg ggctttacgt caaagtggtc atggtgttgt agcagctatt gttcaagcta
     1081 tctctgttat cggtatgttt acagcattaa ttggtatgat gttagcaggc tcacgtttac
     1141 tttattcatt tggacgtgac ggcttattac cttcatggtt aagccattta aacgacaaac
     1201 atttacctaa tcgcgcacta gttatactta ctattattgg tgttttaatt ggttctatgt
     1261 tcccattcgc atttttagca caattaattt cagcaggtac acttgttgca tttatgttcg
     1321 tttcattagc aatgtatcgt ttgagaaagc gtgaaggtaa agatctacca attcctgcat
     1381 ttaaattacc tttatatcct gtgttaccag caattacatt tgttttagta ttgctagtat
     1441 tctggggatt aggattcgaa gcgaaattat acactttaat ttggtttatt gttggtatca
     1501 ttctatattt aagttatgga cttagacatt ctaagaaaaa tgatgtagct gaataccacc
     1561 caccaaaata aaaaatcaaa aaaatcattt cccatctgat taacatccag atgtggaaat
     1621 gattttttat tttatattaa gataatcgct attaccaacc ttgtccagat atgtcatatt
     1681 gatctaagtt acctgcttcc aaagcagtca gtgcatcttc tatagtgttt aacgccgtgt
     1741 ctaattgctc atatgttatc accaatggcg gttggaatcg caacacattt cctgctacag
     1801 ctataataac tacgccatgc tcaaagcagt aattacaaat tttaagtgcc gcactggcat
     1861 cacgtgtttt gagttttttg tcggaaacaa tatcaatacc aatgctcaga cctttacctc
     1921 taacatcgcc tacactattg tatttagata cccattgatc cattcgtttc ctaacatatt
     1981 cccctttttc cgcactagcc tgaagaagcg actgatcttc aatcatttga attgtggcta
     2041 atgcagcttc acaactaact ggattagcac ctgttgtaaa taaatgtgct ggtgcttcta
     2101 aacaattcat aatctctttg cgtccaacaa ttgctgacat gggcatacca cctgctaagg
     2161 attttccgaa agtgattaaa tcaggcgtaa aattaaaatg cgagactgaa ctccatgtac
     2221 ctgttcgccc aaaaccttgt tgaatatcat cgacagcgat taaaatacca tgttcacgac
     2281 aaatcttttc taacgcttca aaataccctg gaactggttc taaaagtcca ccatcgcctt
     2341 gtatcgtttc aataacaata catgctactt cgtcagcagg tacatatttc gcaaacattt
     2401 cttttaaggg tgctaaatat tcttctactg aattagcttg tggctgctcg tacataccac
     2461 gatatttatc tggaaacgga atatgataaa aaccattcaa taacggacca taatgtttgc
     2521 gcatatttaa actaatagct gacatagaca atgagccaaa agttgaacca tgatatgcat
     2581 tagtgaaact aatgatataa ggacgccctg tatatgctct ggcaaattta atgatgccat
     2641 catttgcgtc tgatccggtt aatccgaagg tcactctttt ttcaaaatct ccaggtgcaa
     2701 gctcacaaag cttcttagct aaacgtacca acggttcatg atacatatat gctggtgtat
     2761 aatgaataaa tttatcaact tgtgccttta tcgcttctgt tacttctcta ggtgcatgac
     2821 ctacgttttg agaacttgca ctcgataaca aatcgatata tgtcttcccc tcaatatcaa
     2881 ccaatgttgc tccatatcca tgatcaatca ctaacggata atatttaata cgtcctgatt
     2941 tcgcaaaata atgttcatcc tcttgaatta attgatgtgc tttactcata tacattcccc
     3001 tcccattgac agtattaatg tgcaagcaaa gtggcctctc ttaaatcgat tataaaatcc
     3061 gatttaaaat cctgctcaaa taccgtcatg cctctatgaa tactatccaa tatactaata
     3121 aaattcagat ttgtaaatat attctgataa ttaagaattt tatatcaatc tactttgttt
     3181 atgcgcataa ttttgttcga ttagggaatt gtttactata ttccatttga ctctgaggtg
     3241 atacgaatgc gtttaaaagt attgttccat ttcattgctg ctattttcat atcattcatg
     3301 ttgctatgga tgaccatgtt gtttgactta ataagcaatc aatcacattt aaaagcttta
     3361 ttattgaatt tagattttct aattccttct gacaatacac catatattct agaaataata
     3421 tgtcacttac taattggtag cgtcatttat tttgtatttg tgttgctgtt tcatacttct
     3481 aaaaggcttt actatctatg ttatatccct ttatttttct tatttatagc attgtatccc
     3541 tttttagtat ttattgcgca acgtccaatt tttcaattta gtgtgacaga attaatcggt
     3601 tggattatta cacatatttt ctttatgtca ttaatggcgt tagtcatacc tagaataaaa
     3661 taatattttt aattaaactg acaattattt ttcacatttt atacactttt caaggtttat
     3721 gacttgagaa atgttctcat ttttatcata ctggaggtgt attgaaaagt taaggagtgg
     3781 aaagtatgaa taaagagcaa ttagaaaaaa tgaaaaatgg aaaaggcttt attgccgcat
     3841 tagaccaaag tggtggtagt acaccaaaag cacttaaaga atatggtgta aacgaagatc
     3901 aatatagcaa tgaagacgaa atgttccaac ttgttcacga tatgcgtaca cgtgtggtaa
     3961 cttcaccttc attctcacca gataaaattt tgggtgctat tcttttcgaa caaacaatgg
     4021 atcgcgaagt agaaggcaaa tacactgcag attacttagc tgataaaggt gttgttccgt
     4081 tcttaaaagt agacaaaggt cttgctgaag agcaaaatgg tgttcaatta atgaaaccaa
     4141 tcgacaactt agacagttta ttagaccgtg caaacgaacg tcacattttt ggtacaaaaa
     4201 tgcgttctaa cattttagaa ttaaatgagc aaggtatcaa agacgttgtt gaacaacaat
     4261 ttgaagttgc taaacaaatt attgctaaag gtttagtgcc aattatcgaa ccagaagtta
     4321 atattaatgc aaaagacaaa gctgaaattg aaaaagtatt aaaagctgaa cttaaaaaag
     4381 gtttagatag cttaaatgct gatcaattag taatgttgaa attaacaatt cctactgaac
     4441 caaacttata caaagagtta gctgaacatc ctaatgttgt tcgtgtcgtt gtattatcag
     4501 gtggttacag cagagaaaaa gcaaatgaat tacttaaaga taacgatgaa cttatcgcaa
     4561 gtttctcacg tgcattagct agtgatttaa gagctgatca atctaaagaa gaattcgata
     4621 aagctttagg tgatgctgta gaatcaatct acgacgcatc tgtaaacaaa aactaattta
     4681 tacattaaac gtattttttg atataaacta ctaaaaagga ctcatacatg tgctttaagg
     4741 gcattgtgta tgagtctttt ttgattaaag ttgaatttgc cgtatgtatt agattacgta
     4801 aaaaaatcgg acagctgaaa aatctttaag atttctcagt tgtccgattt taaatatagc
     4861 tattagaatt tagttttcgt agtaacctaa ttctaagtct tttgaagttt gtttacggat
     4921 ttttctcatt aatttttcgt cttcaattaa tgattcaccg tatgatggaa tcattttctt
     4981 gattttaggt gcccattcag ttttgtattc agggaagtta cgttctaata cttctaacgc
     5041 aactgaaact gaagttgaag cccctggtga ttcacctaat aatgcaatta cagtgtggtc
     5101 ttgtgagtta accacttctg taccgaattg gatgaatcct ttaccgtgtt caggtgtatc
     5161 tttgataact tgtacacgtt taccagcagt gtataattgc caatcttcat tacgtgcttc
     5221 tggatagaaa gtacgtaagt ggttcataca accttctttt gtcataatca cttgatcaaa
     5281 tgagtattta attaaaggta agtttttaac tgctgctgct aataaagttg taatgttgta
     5341 tgttttaaca gacttgaata aatctaagtt agaaccattt ttcaagaatt taggtccaac
     5401 attagcaaat ggtccaaata ataatgttct ttgaccatca atgtaacgcg tatctaaatg
     5461 aggtacagtc attggtggtg taccaggtgg ctctttacca taaactttgg catcgtgttg
     5521 ttcaataact tgtgggtttg tacaagctaa gaattgacca ctgataggga atccacccaa
     5581 atgtttactt tcagggatac ctgttttttg taataatgga atcgctccac cgccagcacc
     5641 gatgaataca tagtcagtta cttgtttgaa tttctcacca gttaggcgat ttttaacagt
     5701 aacttcccat tgaccatttg ataattgttc aaaatcaaca acttcatggt taaattgcac
     5761 tgtagcattt ggatgtgctt caatgctttt agccatttta cgtgttaatt caccgaagtt
     5821 tacatctgta ccttcgtcaa ttttacttgc cgccatgata ccagggttat cttcacggcc
     5881 tttcatcatc aatggaatcc attttttcat tacttcgatg tcttcagtat attcgatatt
     5941 atcgaacata gggaaagctt tcatcgcttc gtaacgatct tttaagaatt taacattgtt
     6001 tttacctcta acataactga tgtgtggtaa tggattgata aattctcttg ggttctcgat
     6061 gctaccgctt ttcactaagt gaccccagaa ttgttttgaa atctcaaact cttcgttaat
     6121 cactttcgct ttttcgatgt cgatagaacc atcaggttgt aaaactgtgt agttcaactc
     6181 acataatgct gcatgacccg taccagcatt atttctttcg tttgaacttt cgattgcagg
     6241 acgatccaag cgttcgtaaa cgtggatatt ccagtctggc tcaatttctt ttaacattga
     6301 accaaatgtt gtgctaagta caccggctcc aattaaaacg atgtctttac tattagactt
     6361 agccattggt ttcacctctc caaaaattgt aaatgtgtat catcaatatg aaagttacat
     6421 aaaactgaca tatttcttta aaatatcaac gccatagata acttcctgtt ttaattgata
     6481 cgctgtaaca
//
LOCUS       DAHHSC010000135         6490 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.136, whole genome shotgun sequence.
ACCESSION   DAHHSC010000135 DAHHSC010000000
VERSION     DAHHSC010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 6490)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6490)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6490
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.136"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            121..780
                     /gene="arlR"
                     /locus_tag="NBI57_001942"
     CDS             121..780
                     /gene="arlR"
                     /locus_tag="NBI57_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor ArlR"
                     /protein_id="HCD1822876.1"
                     /translation="MTQILIVEDEQNLARFLELELTHENYNVDTEYDGQDGLDKALSH
                     YYDLIILDLMLPSINGLEICRKIRQQQSTPIIIITAKSDTYDKVAGLDYGADDYIVKP
                     FDIEELLARIRAILRRQPQKDIIDVNGITIDKNAFKVTVNGAEIELTKTEYDLLYLLA
                     ENKNHVMQREQILNHVWGYNSEVETNVVDVYIRYLRNKLKPYDRDKMIETVRGVGYVI
                     R"
     gene            777..2132
                     /gene="arlS"
                     /locus_tag="NBI57_001943"
     CDS             777..2132
                     /gene="arlS"
                     /locus_tag="NBI57_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase ArlS"
                     /protein_id="HCD1822877.1"
                     /translation="MTKRKLRNNWIIVTTMITFVTIFLFCLIIIFFLKDTLHNSELDD
                     AERSSSDINNLFHSKPVKDISALDLNASLGNFQEIIIYDEHNNKLFETSNDNTVRVEP
                     GYEHRYFDRVIKKRYKGIEYLIIKEPITTQDFKGYSLLIHSLENYDNIVKSLYIIALA
                     FGVIATIITATISYVFSTQITKPLVSLSNKMIEIRRDGFQNKLQLNTNYEEIDNLANT
                     FNEMMSQIEESFNQQRQFVEDASHELRTPLQIIQGHLNLIQRWGKKDPAVLEESLNIS
                     IEEMNRIIKLVEELLELTKGDVNDISSEAQTVHINDEIRSRIHSLKQLHPDYQFDTDL
                     TSKNLEIKMKPHQFEQLFLIFIDNAIKYDVKNKKIKVKTRLKNKQKIIEITDHGIGIP
                     EEDQDFIFDRFYRVDKSRSRSQGGNGLGLSIAQKIIQLNGGSIKIKSEINKGTTFKII
                     F"
     gene            2416..5214
                     /locus_tag="NBI57_001944"
     CDS             2416..5214
                     /locus_tag="NBI57_001944"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HCD1822878.1"
                     /translation="MTNERKEVSEAPVNFGANLGLMLDLYDDFLQDPSSVPEDLQVLF
                     STIKNDDSIVPALKSTSSQNSDGTIKRVMRLIDNIRQYGHLKADIYPVNPPKRKHVPK
                     LEIEDFDLDQQTLEGISAGIVSDHFADIYDNAYEAILRMEKRYKGPIAFEYTHINNNT
                     ERGWLKRRIETPYKVTLNNNEKRALFKQLAYVEGFEKYLHKNFVGAKRFSIEGVDALV
                     PMLQRTITIAAKEGIKNIQIGMAHRGRLNVLTHVLEKPYEMMISEFMHTDPMKFLPED
                     GSLQLTAGWTGDVKYHLGGIKTTDSYGTMQRIALANNPSHLEIVAPVVEGRTRAAQDD
                     TQRAGAPTTDHHKAMPIIIHGDAAYPGQGINFETMNLGNLKGYSTGGSLHIITNNRIG
                     FTTEPIDARSTTYSTDVAKGYDVPIFHVNADDVEATIEAIDIAMEFRKEFHKDVVIDL
                     VGYRRFGHNEMDEPSITNPVPYQNIRKHDSVEYVFGKKLVNEGVISEDEMHSFIEQVQ
                     KELRQAHDKINKADKMDNPDMEKPAELALPLQADEQSFTFDHLKEINDALLTYPDGFN
                     ILKKLNKVLEKRHEPFNKEDGLVDWAQAEQLAFATILQDGTPIRLTGQDSERGTFSHR
                     HAVLHDEQTGETYTPLHHVPDQKATFDIHNSPLSEAAVVGFEYGYNVENKKSFNIWEA
                     QYGDFANMSQMIFDNFLFSSRSKWGERSGLTLFLPHAYEGQGPEHSSARLERFLQLAA
                     ENNCTVVNLSSSSNYFHLLRAQAASLDSEQMRPLVVMSPKSLLRNKTVAKPIDEFTSG
                     GFEPILTESYQADKVTKVILATGKMFIDLKEALAKNPDESVLLVAIERLYPFPEEEIE
                     ALLAQLPNLEEVSWVQEEPKNQGAWLYVYPYVKVLVADKYDLSYHGRIQRAAPAEGDG
                     EIHKLVQNKIIENALKNN"
     gene            5228..>6490
                     /gene="sucB"
                     /locus_tag="NBI57_001945"
     CDS             5228..>6490
                     /gene="sucB"
                     /locus_tag="NBI57_001945"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HCD1822879.1"
                     /translation="MPEVKVPELAESITEGTIAEWLKNVGDSVEKGEAILELETDKVN
                     VEVVSEEAGVLSEQLASEGDTVEVGQAIAIIGEGSGNASKENSNDNTPQQNEETNNKK
                     EETTNNSVDKAEVNQANDDNQQRINATPSARRYARENGVNLAEVSPKTNDVVRKEDID
                     KKQQAPASTQTTQQAPAKEEKKYNQYPTKPVIREKMSRRKKTAAKKLLEVSNNTAMLT
                     TFNEVDMTNVMELRKRKKEQFMKDHDGTKLGFMSFFTKASVAALKKYPEVNAEIDGDD
                     MITKQYYDIGVAVSTDDGLLVPFVRDCDKKNFAEIEAEIANLAVKAREKKLGLDDMVN
                     GSFTITNGGIFGSMMSTPIINGNQAAILGMHSIITRPIAIDQDTIENRPMMYIALSYD
                     HRIIDGKEAVGFLKTIKELIENPEDLLLE"
BASE COUNT         2441 a          956 c         1206 g         1887 t
ORIGIN      
        1 ttgatttgtg ataaaattta aacgtaaaca aactaattta aaaagcaact attgtataga
       61 aaaatacaaa atttaaaata tattacctta ttagaaaaag tcgtaatatg aggtgtacaa
      121 atgacgcaaa ttttaatagt agaagatgaa caaaacttag caagatttct tgaattggaa
      181 ctcacacatg aaaattacaa tgtggacaca gagtatgatg gacaagacgg tttagataaa
      241 gcgcttagcc attactatga tttaatcata ttagatttaa tgttgccgtc aattaatggc
      301 ttagaaattt gtcgcaaaat tagacaacaa caatctacac ctatcattat aattacagcg
      361 aaaagtgata cgtatgacaa agttgctggg cttgattacg gtgcagacga ttatatagtt
      421 aagccgtttg atattgaaga acttttagca agaattcgtg caattttacg tcgtcagcca
      481 caaaaggata ttatcgatgt caacggtatt acaattgata agaacgcttt taaagtgacg
      541 gtaaatggcg cagaaattga attaacaaaa acagagtatg atttactata tcttctagct
      601 gaaaataaaa accatgttat gcaacgggaa caaattttaa atcatgtatg gggttataat
      661 agtgaagtag aaacaaatgt cgtagatgtt tatataagat atttacgaaa caagttaaaa
      721 ccatacgatc gtgacaaaat gattgaaaca gttcgtggcg ttgggtatgt gatacgatga
      781 caaaacgtaa attgcgcaat aactggatta ttgttaccac gatgattacg tttgtcacga
      841 tatttttgtt ttgtttaatt attatttttt tcttgaaaga tacactgcat aatagtgagc
      901 ttgatgatgc agaacgaagc tcaagcgata ttaataattt atttcattct aagcctgtta
      961 aagatatatc tgcattagac ttgaatgcat ctttaggtaa ttttcaagag ataattattt
     1021 atgatgagca taataataaa ttatttgaga catcgaatga taacacagtg agagttgaac
     1081 caggttatga acaccgttat tttgaccgcg taataaaaaa acgctataaa ggcattgaat
     1141 atttaattat taaagaacca attacaacgc aagatttcaa agggtatagc ttgttaattc
     1201 attcactaga aaattatgat aacatcgtaa aatcattgta tatcattgcg ctggcatttg
     1261 gagtgattgc aacaattata actgccacaa tcagttatgt attttcaaca caaattacta
     1321 aaccgcttgt cagtttatca aataaaatga ttgagattcg acgagatggt tttcaaaata
     1381 aattgcaatt aaatacaaat tatgaagaaa tagataattt agcaaatacg tttaatgaga
     1441 tgatgagcca aattgaagaa tcatttaatc aacaaagaca atttgttgaa gatgcgtcac
     1501 atgaattacg aacaccatta caaattattc aaggtcattt aaatttgatt cagcgatggg
     1561 gaaaaaaaga cccagcagta ttagaagaat cgttaaatat ttctattgaa gaaatgaatc
     1621 gtatcataaa attagtcgaa gaattacttg aattgactaa aggagatgta aatgacattt
     1681 cttctgaagc gcagaccgtg catattaatg atgaaattcg ctcgcgaata cactcattaa
     1741 aacaattgca tcctgattat caatttgata cggatctgac atctaaaaat ctagaaatta
     1801 aaatgaaacc tcatcaattc gaacaattat ttttaatctt tattgataat gcaatcaaat
     1861 atgatgtgaa gaataagaaa attaaagtta agacaaggtt aaaaaataag caaaaaataa
     1921 ttgaaattac agatcatgga attggtattc cagaggaaga tcaagatttc atttttgatc
     1981 gcttttatcg agtggataaa tctcgttcaa gaagtcaagg cggtaatgga ctcggattat
     2041 ctattgctca aaaaatcatt caattaaacg gaggatcgat taaaattaaa agtgaaatta
     2101 ataaaggaac aacgtttaaa atcatatttt aatcatgtct gagacgtcaa tcaaagtcat
     2161 aggatcaatt ttttaagtac acattagctg tgactaatgt ataagaacaa ctataaaaca
     2221 aataaacagt ggttctttat catttctgtt gtactcccaa aatttacaat aaaatacatc
     2281 tataaaccta gaagaatcaa cgcttttgtt gattcttctt tttagcagat aaataggtaa
     2341 atctacttta acaaataact aaatagtgat attattacat tgtaagcgtt tcaacatttt
     2401 tgtggagggt gtaaaatgac taacgaaaga aaagaagttt cagaggctcc tgtaaacttc
     2461 ggtgcgaatt taggtctaat gttagatcta tatgatgact ttttacaaga tccatcatct
     2521 gtaccagaag atttacaagt cttattcagc acaattaaga atgatgactc aattgtacca
     2581 gctttaaaaa gtacaagtag tcaaaatagc gacggcacaa ttaagcgtgt catgcgtcta
     2641 attgataata ttcgccaata cgggcatctt aaagccgata tttatcctgt aaatcctcca
     2701 aaaaggaaac atgtacctaa attagagatt gaagactttg atttagatca acagactttg
     2761 gaaggtatat cagcaggaat tgtttcagat cactttgccg acatttatga taatgcttat
     2821 gaagcaattt taagaatgga aaaacgttac aaaggaccaa ttgcatttga gtatacacat
     2881 attaataaca ataccgaacg tggttggtta aaaagaagaa ttgaaacgcc atataaagta
     2941 acgttaaata ataacgaaaa aagggcacta ttcaaacaat tagcgtatgt tgaagggttt
     3001 gaaaaatatc ttcataaaaa cttcgttggt gcaaagcgtt tttcaattga aggggtagac
     3061 gcacttgtac cgatgttaca acgtactatt acgattgctg cgaaagaagg tattaaaaat
     3121 atacaaatag gcatggctca ccgtggacgt ttaaacgttt taacgcatgt cttagaaaaa
     3181 ccgtacgaaa tgatgatttc agaatttatg catacagatc caatgaaatt cttacctgaa
     3241 gatggtagct tgcagttaac tgctggatgg actggtgatg tgaaatatca ccttggtggc
     3301 attaaaacta ctgattcata cggtacaatg cagcgtattg cactggctaa caatccaagt
     3361 cacttggaaa ttgttgcacc tgttgttgag gggcgtacga gagcagcaca agatgataca
     3421 caacgagctg gggctccgac gactgatcat cataaagcaa tgccaattat tatacatggc
     3481 gatgctgctt atcctggtca aggaattaac ttcgaaacaa tgaacttagg aaacttgaaa
     3541 ggctattcta cgggtggttc attgcatatt attactaaca atagaattgg atttactaca
     3601 gaaccaattg atgcacgttc aacaacttat tctacagatg tggccaaagg ttatgatgtg
     3661 ccaatattcc atgtcaatgc tgatgacgtt gaagctacta ttgaagcaat tgatattgca
     3721 atggaattta gaaaagagtt tcataaagac gtcgttattg atttagtagg ttatcgtcgt
     3781 ttcggacata acgaaatgga tgaaccatca attactaatc cagttcctta tcagaatatt
     3841 cgcaaacatg actctgttga atatgtgttt ggtaaaaagc ttgttaatga aggtgtcatt
     3901 tcagaagatg aaatgcattc atttatagaa caagtccaaa aggaactaag acaagctcat
     3961 gataaaatta ataaagctga taaaatggat aatccagata tggaaaaacc tgcagaactt
     4021 gcattaccgt tacaagcaga cgaacaatca tttacgtttg atcatttgaa agaaataaat
     4081 gatgcattgt taacatatcc ggatggcttt aacattttga aaaagttaaa caaagttctt
     4141 gagaagcgtc atgagccgtt taataaagaa gatggcttag ttgattgggc acaagcagaa
     4201 caacttgcat ttgcgacaat tttacaagat ggtacaccga ttcgcttaac tggtcaagat
     4261 agtgaacgtg gtacattcag tcataggcat gccgtgttac atgatgagca aacaggtgaa
     4321 acatatacac ctttacatca tgttcctgat caaaaagcga catttgatat acacaattct
     4381 ccgctttcag aagcagcagt agttggtttt gaatacggct ataatgtgga aaacaaaaaa
     4441 agcttcaata tttgggaagc gcaatatggt gattttgcaa atatgtcaca aatgattttt
     4501 gacaacttct tattcagttc tcgctcaaaa tggggagaac gttcaggatt aacattattc
     4561 ttacctcatg catatgaggg tcaaggacct gaacattcat cagcaagatt agagcgattt
     4621 ttacaattag ctgctgaaaa taattgcaca gttgtcaact tatctagttc aagtaattat
     4681 ttccacttat tgcgtgcaca agcagctagt ttagattctg aacaaatgcg accattggtt
     4741 gttatgtcac caaaaagctt attgagaaat aaaacagttg ccaaaccaat tgatgagttt
     4801 acttctggtg gatttgagcc aattttgaca gaatcatatc aagcggataa ggttacaaaa
     4861 gttattttag caactggtaa aatgttcatt gatttaaaag aagcattagc taaaaatcca
     4921 gacgaatccg tgttactcgt tgcgattgaa agattgtatc cattcccaga ggaagagatt
     4981 gaagcattac tagcacaatt gccaaacctt gaagaagtgt catgggtaca agaagaacct
     5041 aaaaatcaag gtgcatggtt atatgtctat ccatatgtta aagtgctagt tgcagataaa
     5101 tatgatttaa gttatcatgg cagaattcaa agggctgctc cagctgaagg cgatggagaa
     5161 attcataaac ttgttcaaaa taaaattata gaaaatgcat taaaaaataa ctagggggaa
     5221 ataagtcatg ccagaggtta aagttccaga attagcagaa tctattacag aaggtaccat
     5281 tgcagaatgg ttgaaaaacg taggggatag cgtagaaaaa ggtgaagcta ttcttgaatt
     5341 agaaactgat aaagttaatg tcgaagttgt atctgaagaa gcaggtgtat tatctgaaca
     5401 acttgcaagt gaaggcgaca ctgtagaagt tggacaagca attgctatca tcggcgaagg
     5461 tagtggcaat gcttctaaag aaaatagtaa cgacaatact ccacaacaaa atgaagaaac
     5521 aaataataaa aaagaagaaa caacaaataa ttcggtagat aaagctgaag tcaatcaagc
     5581 aaatgatgac aatcagcaac gtattaatgc tacgccttct gcgcgtcgat atgctcgtga
     5641 aaatggtgtg aatcttgctg aagtaagtcc gaaaacaaat gatgtggttc gtaaagaaga
     5701 tattgataag aaacaacagg caccggcatc aacacaaaca acacaacaag cacctgcaaa
     5761 agaagagaaa aaatacaatc aatatcctac aaaaccagtg attcgtgaaa aaatgtcacg
     5821 cagaaagaaa acagctgcca aaaaattatt agaggtatct aataatacag ctatgttaac
     5881 aacatttaac gaagttgaca tgacaaatgt tatggaattg cgtaaacgta agaaagaaca
     5941 atttatgaaa gatcatgatg gtactaaatt aggatttatg tcattcttta ctaaagcttc
     6001 tgtagcagct ttgaaaaagt atccagaagt taatgcagaa atcgatggcg acgacatgat
     6061 tacgaaacaa tattatgata ttggtgtagc tgtttctaca gatgatggat tattagtacc
     6121 atttgtaaga gattgtgata aaaagaattt tgcagaaatc gaagcagaaa ttgctaattt
     6181 agcagttaaa gcacgagaga aaaaacttgg cttagatgat atggttaatg gttcatttac
     6241 gattacaaat ggcggtattt ttggatcaat gatgagtacg ccaattatca atggtaatca
     6301 agctgcaatc ttaggcatgc attcaattat tacaagacca attgcgattg atcaagatac
     6361 aatcgaaaat cgtccaatga tgtatattgc attaagctat gatcatagaa ttattgacgg
     6421 taaagaagca gttggattct taaaaacaat taaagaatta attgaaaacc cagaagactt
     6481 attattagaa
//
LOCUS       DAHHSC010000136         6483 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.137, whole genome shotgun sequence.
ACCESSION   DAHHSC010000136 DAHHSC010000000
VERSION     DAHHSC010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 6483)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6483)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6483
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.137"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            complement(140..2128)
                     /gene="tkt"
                     /locus_tag="NBI57_001946"
     CDS             complement(140..2128)
                     /gene="tkt"
                     /locus_tag="NBI57_001946"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001661967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HCD1822880.1"
                     /translation="MFNEKDQLAVDTLRALSIDTIEKANSGHPGLPMGAAPMAYTLWT
                     RHLNFNPQSKDYFNRDRFVLSAGHGSALLYSLLHVSGSLELEELKQFRQWGSKTPGHP
                     EYRHTDGVEVTTGPLGQGFAMSVGLALAEDHLAGKFNKEGYNVVDHYTYVLASDGDLM
                     EGISHEAASFAGHNKLSKLVVLYDSNDISLDGELNKAFSENTKARFEAYGWNYLLVKD
                     GNDLEEIDKAITTAKSQEGPTIIEVKTTIGFGSPNKAGTNGVHGAPLGEVERKLTFEN
                     YGLDPEKRFNVSEEVYEIFQNTMLKRANEDESQWNSLLEKYAETYPELAEEFKLAISG
                     KLPKNYKDELPRFELGHNGASRADSGTVIQAISKTVPSFFGGSADLAGSNKSNVNDAT
                     DYSSETPEGKNVWFGVREFAMGAAVNGMAAHGGLHPYGATFFVFSDYLKPALRLSSIM
                     GLNATFIFTHDSIAVGEDGPTHEPIEQLAGLRAIPNMNVIRPADGNETRVAWEVALES
                     ESTPTSLVLTRQNLPVLDVPEDVVEEGVRKGAYTVYGSEETPEFLLLASGSEVSLAVE
                     AAKDLEKQGKSVRVVSMPNWNAFEQQSEEYKESVIPSSVTKRVAIEMASPLGWHKYVG
                     TAGKVIAIDGFGASAPGDLVVEKYGFTKENILNQVMSL"
     gene            complement(2249..2488)
                     /locus_tag="NBI57_001947"
     CDS             complement(2249..2488)
                     /locus_tag="NBI57_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 domain-containing protein"
                     /protein_id="HCD1822881.1"
                     /translation="MSNSDLNIERINELAKKKKEVGLTQEEAKEQTALRKAYLESFRK
                     GFKQQIENTKVIDPEGNDVTPEKIKEIQQKRDNKN"
     gene            complement(2625..2858)
                     /locus_tag="NBI57_001948"
     CDS             complement(2625..2858)
                     /locus_tag="NBI57_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1822882.1"
                     /translation="MFYNKYKNVSTYIIIFLVSSAAFAIFLLSANVSAHSEQVYEMTD
                     HQIKNNTINKAYEHKDPTNNGEQRDGKVFALIN"
     gene            3001..3624
                     /gene="lexA"
                     /locus_tag="NBI57_001949"
     CDS             3001..3624
                     /gene="lexA"
                     /locus_tag="NBI57_001949"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HCD1822883.1"
                     /translation="MRELTKRQSEIYNYIKQVVQMKGYPPSVREIGEAVGLASSSTVH
                     GHLSRLEEKGYIRRDPTKPRAIEIVSDQTNDNINMEETIHVPVIGKVTAGVPITAVEN
                     IEEYFPLPEHLTSTHNSDIFILNVVGDSMIEAGILDGDKVIVRSQTIAENGDIIVAMT
                     EEDEATVKRFYKEKNRYRLQPENSTMEPIYLDNVAVIGKVIGLYREM"
     gene            complement(4004..5020)
                     /locus_tag="NBI57_001950"
     CDS             complement(4004..5020)
                     /locus_tag="NBI57_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001789887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HCD1822884.1"
                     /translation="MKKFIVTVVAFLSIIIIAPITEFKPFIHLQNEVRQYIDIHINKE
                     TISAENKLDTPKKQQFAFNNIQMNMSKSDVEKTLNKPKRVTFNEYGTKWYTYYDDDYN
                     NFIMISYMKDKVNALYTNQNIITSKSKIKYNTPKSVVRQRLGEPETEIVKGRVRYEQN
                     NKEYDVFHKNHIYTTVFYDKHRRNNVTAVLQVSDAMENRLKEQYGAPSKSLADSFELQ
                     NFDLVNAERKQHQLSTLKYSKQNSETARKHSKDMAKNHYFDHTNLKGQSPFDRLKKDG
                     ITFNSAGENLAYGQVSSIYAHQGLMNSIGHRKNILNDTFKILGVGVDFNDEKQPFWTE
                     NYTG"
     gene            complement(5042..6019)
                     /gene="guaC"
                     /locus_tag="NBI57_001951"
     CDS             complement(5042..6019)
                     /gene="guaC"
                     /locus_tag="NBI57_001951"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015365036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HCD1822885.1"
                     /translation="MKIFDYEDIQLIPNKCIVESRSECDTTIQFGPKKFKLPVVPANM
                     QTVMNEKLAKWFAENDYFYIMHRFDEEARIPFIKHMQNSGLFASISVGVKKAEFDFIE
                     KLAQEKLIPEYITIDIAHGHSDSVINMIKHIKNHIPDSFVIAGNVGTPEGVRELENAG
                     ADATKVGIGPGRVCITKIKTGFGTGGWQLAALNICSKAARKPLIADGGIRTHGDIAKS
                     IRFGASMVMIGSLFAAHEESPGETVELDGKQYKEYFGSASEFQKGEHKNVEGKKMFVE
                     HKGSLMDTLKEMQQDLQSSISYAGGKDLKSLRTVDYVIVRNSIFNGDRD"
     gene            complement(6176..6445)
                     /gene="rpsN"
                     /locus_tag="NBI57_001952"
     CDS             complement(6176..6445)
                     /gene="rpsN"
                     /locus_tag="NBI57_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009384394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HCD1822886.1"
                     /translation="MAKKSKIAKERKREELVNKYYELRKELKAKGDYEALRKLPRDSS
                     PTRLTRRCKVTGRPRGVLRKFEMSRIAFREHAHKGQIPGVKKSSW"
BASE COUNT         2026 a         1173 c          901 g         2383 t
ORIGIN      
        1 attaatctga aatagtatac tttcataact tatcacttat gatgtagttt aagtgctata
       61 ctctaaattt caacacttta aaagaagcct aagattatgc atcctagact tctaaacata
      121 ctcgctttat aaattattct tataagctca taacttggtt taagatattt tctttagtaa
      181 atccatattt ttcaactact aaatcgccag gtgcacttgc gccaaagccg tcaatagcaa
      241 taactttacc tgcagtacct acatatttat gccatccaag cggtgaagcc atttcaatcg
      301 caacacgttt tgttacgctt gatggaataa ctgattcttt atattcttca gattgttgtt
      361 caaatgcatt ccagttaggc attgaaacaa cacgcactga tttaccttgt ttttcaagat
      421 ctttagcagc ttcaactgca agactaactt ctgaacctga agctaataat aggaattctg
      481 gtgtctcttc agagccataa actgtatagg caccttttcg aacgccttct tcaactacat
      541 cttctggtac atctaaaacc ggtaagtttt gacgtgtcaa tactaatgaa gtaggtgtag
      601 attcagattc taaggcaact tcccatgcta ctcttgtttc attaccatca gcaggacgga
      661 taacattcat atttggaata gctcttaatc cagctaattg ttcaattggt tcatgagtag
      721 gaccatcttc acctactgca attgaatcat gtgtgaagat gaacgttgca tttaatccca
      781 taattgatga taaacgtaac gctggtttta aataatcact aaatacgaag aatgttgcac
      841 catatggatg taaacctcca tgtgcagcca taccatttac agcagcaccc atagcaaatt
      901 cacgtacacc aaaccacaca tttttacctt caggtgtttc agaactataa tctgttgcat
      961 catttacatt ggatttgttt gaaccagcaa ggtctgctga tccaccaaag aatgaaggga
     1021 cagttttact gattgcttga ataacagtac cagaatcagc acgagatgca ccattatgac
     1081 ccagttcaaa acgtggtaat tcatccttat aatttttagg caatttacca ctaatcgcta
     1141 atttaaattc ttctgctaat tcaggatatg tttctgcata tttttctaat aatgaattcc
     1201 attgagattc atcttcatta gcacgtttta acatagtatt ttggaaaatt tcgtatacct
     1261 cttctgaaac attaaaacgt ttttcaggat ctaaaccgta attttcgaat gttaattttc
     1321 tttcaacttc acctaaaggt gccccatgaa caccattagt tcctgcttta ttcggtgaac
     1381 caaatccgat tgttgtttta acttcaataa tcgttggtcc ttcttgagat ttagctgtag
     1441 taatcgcttt atcaatttct tctaaatcat taccatcttt aactagtaag taattccaac
     1501 catatgcttc aaaacgagct tttgtgtttt cagaaaaagc tttgtttaat tcgccatcta
     1561 atgaaatatc atttgaatcg tataaaacaa ctaatttact taatttatta tgtccagcaa
     1621 atgaagctgc ttcatgcgat ataccttcca ttaaatcacc gtcagaagct aatacatatg
     1681 tgtaatgatc tacaacatta tatccttctt tattaaattt ccctgctagg tgatcttctg
     1741 ctaaagctaa tcctactgac atagcaaaac cttgtccaag tggtccagta gtaacttcta
     1801 caccatcagt atgtctgtat tcaggatggc ctggtgtttt agaaccccat tgtctaaatt
     1861 gctttaattc ttctaattct aaactaccag aaacatgtaa caagctatac aataatgctg
     1921 aaccatgccc tgccgataat acgaaacggt ctctattgaa gtaatcttta gattgtggat
     1981 taaaattcag atgacgtgtc cacaaagtgt aagccattgg ggcagctccc ataggtaatc
     2041 ctggatgacc agaattcgct ttttcgattg tgtcgatact tagtgcacgt agcgtatcaa
     2101 cagctaattg atctttttca ttaaacatct tgtgtatctt cccttcttca attttatcaa
     2161 atacattata acttatttta aaataaaaag ataagtaaaa tacttatttc aagttataat
     2221 gtcagaaaat tctttacaga tttgtgattt aatttttatt atctcttttt tgttgtatct
     2281 ctttaatttt ttcaggtgtt acatcattac cttctggatc aattacttta gtattttcaa
     2341 tttgttgttt aaaccctttt ctaaaactct caagataagc ttttcttaag gctgtttgct
     2401 cctttgcttc ttcttgagtt aatcctactt cttttttctt tttagctaac tcgttaattc
     2461 tttcgatatt caaatcagaa ttactcaatt aaaaaaccta ccttcaattt gtttattcaa
     2521 tcattataat actttaacaa aatataacaa atttaagacc agcaaaaaag taataaaaaa
     2581 acgctatttc gcaaaaatag gcaaaataac gttgtgacaa tgtatcaatt tattaaagcg
     2641 aacactttcc catctctttg ttcgccattg tttgtagggt ctttatgttc gtatgcttta
     2701 tttatcgtat tgttcttaat ttgatggtca gtcatttcgt acacttgttc cgagtgagca
     2761 ctaacgttcg cacttaacaa gaatattgca aaggctgcac ttgaaactaa aaatatgatg
     2821 atatatgttg atacgttttt atatttattg taaaacatca tttccactcc tagaacattt
     2881 gtttgtataa gtaatttatc agaacttatg ttctgcgtca atattttaac gaacaaatgt
     2941 ttgttttggt tgtgaattaa tgatatactg tatttaaatt aatttaggga gtgcctatat
     3001 atgagagaat taacaaaacg acaaagcgaa atatataact atattaaaca agttgttcaa
     3061 atgaaaggtt atccgcctag tgttcgcgaa attggtgaag cagttggctt agcatccagt
     3121 tcaactgttc atggtcatct ttcacgtctt gaagaaaaag gatatataag aagagatcca
     3181 acgaaaccac gtgctataga aattgtaagt gatcaaacaa atgataatat taatatggaa
     3241 gaaacgattc atgtgccagt tattggtaaa gtcacagcag gtgttcctat taccgctgta
     3301 gaaaatattg aagagtattt tccattacct gaacacttaa catcgacaca taatagcgac
     3361 atattcatat taaacgtcgt aggcgacagt atgattgagg ctggtatatt agacggagac
     3421 aaagtaattg ttcgcagtca aaccatagca gaaaatggag acattattgt tgctatgact
     3481 gaggaagatg aagcaactgt caaacgcttc tataaagaaa aaaatcgtta tcgattacaa
     3541 cctgaaaata gtacaatgga gccaatttac ctcgacaatg ttgctgtaat tgggaaagta
     3601 attggtttgt accgcgaaat gtaatatttt aaaccgttat atattatcgt aattgttaaa
     3661 ccctcatttt tataaatttt ggacctcttg aaaaagtttc gttttcaaga ggttttattt
     3721 attctaatct aaattcagtt caaacagaaa ttgcgaattg ttagataatc tcattcttta
     3781 atataaattt tgaattacag attcctgcaa aatgttacac aaattaaaac tcgcccccgt
     3841 aataatttac aggagcgagc cattattatt tttttctttc ttttttatta agtatatctt
     3901 tgacgcgttc aagtatatcc gcatcttttg gtttatctgt tgtcatcatg tttatctact
     3961 tccttacaga caaacattac cctttaaact cataccaaaa acattaacca gtataatttt
     4021 ctgtccaaaa aggttgtttt tcatcattaa aatcaacacc aacacctaat attttaaacg
     4081 tatcatttaa aatatttttt ctgtgaccaa tagaattcat taatccttga tgtgcataga
     4141 tactactaac ttgaccatat gctaaattct ctccggctga gttaaatgta ataccatctt
     4201 ttttcaatcg atcaaatggt gattgacctt ttaaatttgt atgatcaaaa taatgatttt
     4261 tggccatatc tttactatgc ttacgagcag tttcagaatt ctgtttagaa tacttcaatg
     4321 tagataattg atgttgtttt ctttcagcat taactaaatc aaaattttgt agttcaaaac
     4381 tatctgcaag cgatttcgaa ggtgctccat attgttcttt taatctattt tccatagcat
     4441 cacttacttg taaaacagct gttacattat tacgtcgatg cttatcataa aataccgtcg
     4501 tataaatgtg atttttatgg aaaacatcat attctttatt attttgttcg taacgcactc
     4561 tacctttaac aatctctgtt tctggttcgc ctaatctttg ccttacaacc gatttaggtg
     4621 tattgtattt aatttttgat tttgaagtga ttatattttg atttgtatat aacgcattaa
     4681 ctttatcttt catgtaactt atcattataa aattattgta atcgtcatca taatacgtat
     4741 accacttcgt accatattca ttaaatgtca ctctttttgg tttatttaat gttttctcaa
     4801 catctgattt cgacatgttc atttgtatat tattaaaggc aaattgttgt ttcttcggtg
     4861 tatccaattt attttccgca gaaattgttt ctttattgat gtgaatgtca atatattgtc
     4921 ttacttcatt ttgtaaatga atgaatggtt taaattctgt tattggcgca atgataataa
     4981 ttgataaaaa cgcaacaaca gttactatga attttttaat ttacatcacc tactataaat
     5041 attaatctct atcaccgttg aaaatagagt ttctaacaat aacataatct acagtacgta
     5101 atgatttcaa gtcttttcca ccggcatatg aaattgagct ttgtaaatct tgttgcattt
     5161 cttttaaggt atccattaat gaacccttat gttctacaaa cattttttta ccttctacat
     5221 ttttatgttc gcctttttga aattcagatg cactaccaaa atattcttta tactgtttac
     5281 catcaagttc tacagtttca ccaggtgatt cttcgtgtgc cgcaaataat gaaccaatca
     5341 tgaccattga tgcaccaaat ctaattgatt tagcgatgtc gccatgcgtt cttataccac
     5401 catcggcaat caaaggttta cgagctgctt tactacatat gtttaatgcc gctaactgcc
     5461 aaccaccagt accaaaacct gttttaatct ttgtaataca aactctacca ggacctatac
     5521 cgactttggt agcatcagca ccagcatttt ctaattctct aacaccttct ggcgtaccaa
     5581 cattaccagc aataacaaaa ctatcaggta tatggttttt tatatgttta atcatgttta
     5641 tcactgaatc tgagtgacca tgcgcaatat ctattgtaat atattcgggg attaattttt
     5701 cttgagctaa cttttcaata aaatcaaatt ccgctttctt tacaccaact gaaatagatg
     5761 caaataagcc tgaattttgc atatgtttta taaaaggtat tcttgcttct tcatcaaaac
     5821 gatgcatgat ataaaagtaa tcattttcag caaaccattt cgctaatttc tcattcataa
     5881 ctgtttgcat atttgcagga actacaggta gcttgaattt tttcggacca aattggatag
     5941 ttgtatcaca ttcagaccta ctttcaacta tgcatttatt aggtattaat tgaatatctt
     6001 cgtaatcaaa tattttcact ttaaatagcc cctttaaaaa ctaattattt ttaaatgcaa
     6061 attaaaaacc gaatgtttcc ttattaatat gagaaaacat tcagtttata gcattcgtat
     6121 atactgtaca ttgttttatt gatattgtaa atgggctaaa gtgcgaaatt tgattttacc
     6181 aacttgattt tttaacacct ggaatttgtc ctttgtgcgc atgttctcta aacgcaatac
     6241 gagacatttc aaatttacgt aatacacctc taggtcttcc agttacttta catcttctag
     6301 ttaaacgtgt aggtgatgaa tctcttggta attttcttaa cgcttcgtaa tcaccttttg
     6361 cttttaactc tttacgtaat tcgtaatatt tatttactaa ctcttctctt tttctctctt
     6421 ttgctatttt agatttctta gccatatata gttacctcac tttttatcaa atcgtaatta
     6481 tta
//
LOCUS       DAHHSC010000137         6462 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.138, whole genome shotgun sequence.
ACCESSION   DAHHSC010000137 DAHHSC010000000
VERSION     DAHHSC010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 6462)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6462)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6462
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.138"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            90..1055
                     /locus_tag="NBI57_001953"
     CDS             90..1055
                     /locus_tag="NBI57_001953"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HCD1822887.1"
                     /translation="MIFVYALLALVITFVLVPVLIPTLKRMKFGQSIREEGPQSHMKK
                     TGTPTMGGLTFLLSIVITSLVAIIFVDQANPIILLLFVTIGFGLIGFIDDYIIVVKKN
                     NQGLTSKQKFLAQIGIAIIFFVLSNVFHLVNFSTSIHIPFTNVAIPLSFAYVIFIVFW
                     QVGFSNAVNLTDGLDGLATGLSIIGFTMYAIMSFVLGETAIGIFCIIMLFALLGFLPY
                     NINPAKVFMGDTGSLALGGIFATISIMLNQELSLIFIGLVFVIETLSVMLQVASFKLT
                     GKRIFKMSPIHHHFELIGWSEWKVVTVFWAVGLISGLIGLWIGVH"
     gene            1057..2406
                     /locus_tag="NBI57_001954"
     CDS             1057..2406
                     /locus_tag="NBI57_001954"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HCD1822888.1"
                     /translation="MLNYTGLENKNVLVVGLAKSGYEAAKLLSKLGANVTVNDGKDLS
                     QDAHAKDLESMGISVVSGSHPLTLLDNNPIIVKNPGIPYTVSIIDEAVKRGLKILTEV
                     ELSYLISEAPIIAVTGTNGKTTVTSLIGDMFKKSRLTGRLSGNIGYVASKVAQEVKPT
                     DYLVTELSSFQLLGIEKYKPHIAIITNIYSAHLDYHENLENYQNAKKQIYKNQTEEDY
                     LICNYHQRQVIESEELKAKTLYFSTQQEVDGIYIKDGFIVYKGVRIINTEDLVLPGEH
                     NLENILAAVLACILAGVPIKAIIDSLTTFSGIEHRLQYVGTNRTNKYYNDSKATNTLA
                     TQFALNSFNQPIIWLCGGLDRGNEFDELIPYMENVRAMVVFGQTKAKFAKLGNSQGKS
                     VIEANNVEDAVDKVQDIIEPNDVVLLSPACASWDQYSTFEERGEKFIERFRAHLPSY"
     gene            2422..3741
                     /locus_tag="NBI57_001955"
     CDS             2422..3741
                     /locus_tag="NBI57_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HCD1822889.1"
                     /translation="MDDKTKNDQQESNEDKDELELFTRNTSKKRRQRKRSKATHFSNQ
                     NKDDTSQQADFDEEIYLINKDFKKEESNDENNGSASSHANDNNIDDSTDSNIENEDYR
                     YNQEIDDQNESNGIAVGNEQPQSAPKEQNSDSNDEETVTKKERKSKVTQLKPLTLEEK
                     RKLRRKRQKRIQYSVITILVLLIAVILIYMFSPLSKIAHVNINGNNHVSTSKINKVLG
                     VKNDSRMYTFSKKNAINDLEEDPLIKSVEIHKQLPNTLNVDITENEIIALVKYKGKYL
                     PLLENGKLLKGSNDVKINDAPVMDGFKGTKEDDMIKALSEMTPEVRRYIAEVTYAPSK
                     NKQSRIELFTTDGLQVIGDISTISKKMKYYPQMSQSLSRDSSGKLKTRGYIDLSVGAS
                     FIPYRGNTSSQSESDKNVTKSSQEENQAKEELQSVLNKINKQSSKNN"
     gene            3847..5259
                     /gene="ftsA"
                     /locus_tag="NBI57_001956"
     CDS             3847..5259
                     /gene="ftsA"
                     /locus_tag="NBI57_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HCD1822890.1"
                     /translation="MEEHYYVSIDIGSSSVKTIVGEKFHNGINVIGTGQTYTSGIKNG
                     LIDDFDIARQAIKDTIKKASIASGVDIKEVFLKLPIIGTEVYDESNEIDFYEDTEING
                     SHIEKVLEGIREKNDVQETEVINVFPIRFIVDKENEVSDPKELIARHSLKVEAGVIAI
                     QKSILINMIKCVEACGVDVLDVYSDAYNYGSILTATEKELGACVIDIGEDVTQVAFYE
                     RGELVDADSIEMAGRDITDDIAQGLNTSYETAEKVKHQYGHAFYDSASDQDIFTVEQV
                     DSDETVQYTQKDLSDFIEARVEEIFFEVFDVLQDLGLTKVNGGFIVTGGSANLLGVKE
                     LLSDMVSEKVRIHTPSQMGIRKPEFSSAISTISSSIAFDELLDYVTINYHDNEETEED
                     VIDVKDKDNESKLGGFDWFKRKTNKKDTHENEVESTDEEIYQSEDNHQEHKQNHEHVQ
                     DKDKDKEESKFKKLMKSLFE"
     gene            5292..>6462
                     /gene="ftsZ"
                     /locus_tag="NBI57_001957"
     CDS             5292..>6462
                     /gene="ftsZ"
                     /locus_tag="NBI57_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HCD1822891.1"
                     /translation="MLEFEQGFNHLATLKVIGVGGGGNNAVNRMIDHGMNNVEFIAIN
                     TDGQALNLSKAESKIQIGEKLTRGLGAGANPEIGKKAAEESREQIEDAIQGADMVFVT
                     SGMGGGTGTGAAPVVAKIAKEMGALTVGVVTRPFSFEGRKRQTQAAAGVEAMKAAVDT
                     LIVIPNDRLLDIVDKSTPMMEAFKEADNVLRQGVQGISDLIAVSGEVNLDFADVKTIM
                     SNQGSALMGIGVSSGENRAVEAAKKAISSPLLETSIVGAQGVLMNITGGESLSLFEAQ
                     EAADIVQDAADEDVNMIFGTVINPELQDEIVVTVIATGFDDKPTSHGRKSGSTGFGTS
                     VNTSSNATSKDESFTSNSSNAQATDSVSERTHTTKEDDIPSFIRNREERRSRRTRR"
BASE COUNT         2354 a          862 c         1273 g         1973 t
ORIGIN      
        1 aaattttgat tacaactttg tttatttaat tgaaaaattg ataatatatt agtggttaaa
       61 aaagtctgga ttaagaagag gagatagtta tgatttttgt atatgcgtta ttagcgctag
      121 tgattacatt tgttttggta cctgttttaa tacctacatt aaaaaggatg aaatttggtc
      181 aaagtattcg agaagaaggt ccacaaagcc atatgaagaa gactggtaca ccaacgatgg
      241 gtggactaac atttctatta agtattgtga taacgtcttt ggtggctatt atatttgtag
      301 atcaagctaa tccaatcata ctgttattat ttgtgacgat tggttttggg ttaattggtt
      361 ttatagatga ttatattatt gttgttaaaa agaataacca aggtttaaca agtaaacaga
      421 agtttttggc gcaaattggt attgcgatta ttttctttgt tttaagtaat gtgtttcatt
      481 tggtgaattt ttctacgagc atacatattc catttacgaa tgtagcaatc ccactatcat
      541 ttgcatatgt tattttcatt gttttttggc aagtaggttt ttctaatgcg gtaaatttaa
      601 cagatggttt agatggatta gcaactggac tgtcaattat cggatttaca atgtatgcca
      661 tcatgagctt tgtgttagga gaaacggcaa ttggtatttt ctgtatcatt atgttgtttg
      721 cacttttagg atttttacca tataacatta accctgctaa agtgtttatg ggagatacag
      781 gtagcttagc tttaggtggt atatttgcta cgatttcaat catgcttaat caggaattat
      841 cattaatttt tataggttta gtattcgtaa ttgaaacatt atctgttatg ttacaagtcg
      901 ctagctttaa attgactgga aagcgtatat ttaaaatgag tccgattcat catcattttg
      961 aattgatagg atggagcgaa tggaaagtag ttacagtatt ttgggctgtt ggtctgattt
     1021 caggtttaat cggtttatgg attggagtgc attaagatgc ttaattatac agggttagaa
     1081 aataaaaatg tattagtcgt cggtttggca aaaagtggtt atgaagcagc taaattatta
     1141 agtaaattag gtgcgaatgt gactgtcaat gatgggaaag acttatcgca agatgctcat
     1201 gcaaaagatt tagaatctat gggtatttct gttgtaagtg gaagtcatcc attaacattg
     1261 cttgataata atccaataat tgttaaaaat cctggaatac cttatacagt atctattatt
     1321 gatgaagcag tgaaacgagg tttgaaaatt ttaacagaag ttgagttaag ttatctaatc
     1381 tctgaagcac caatcatagc tgtaacgggt acaaatggta aaacgacagt tacttctcta
     1441 attggagata tgtttaaaaa aagtcgctta actggaagat tatccggcaa tattggttat
     1501 gttgcatcta aagtagcaca agaagtaaag cctacagatt atttagttac agagttgtcg
     1561 tcattccagt tacttggaat tgaaaagtat aaaccacaca ttgctataat tactaacatt
     1621 tattcggcgc atctagatta ccatgaaaat ttagaaaact atcaaaatgc taaaaagcaa
     1681 atatataaaa atcaaacgga agaagattat ttgatttgta attatcatca aagacaagtg
     1741 atagagtcgg aagaattaaa agctaagaca ttgtatttct caactcaaca agaagttgat
     1801 ggtatttata ttaaagatgg ttttatcgtt tataaaggtg ttcgtattat taacactgaa
     1861 gatctagtat tgcctggtga acataattta gaaaatatat tagcagctgt gcttgcttgt
     1921 attttagctg gtgtacctat taaagcaatt attgatagtt taactacatt ttcaggaata
     1981 gagcatagat tgcaatatgt tggtactaat agaactaata aatattataa tgattccaaa
     2041 gcaacaaaca cgctagcaac acagtttgcc ttaaattcat ttaatcaacc aatcatttgg
     2101 ttatgtggtg gtttggatcg agggaatgaa tttgacgaac tcattcctta tatggaaaat
     2161 gttcgcgcga tggttgtatt cggacaaacg aaagctaagt ttgctaaact aggtaatagt
     2221 caagggaaat cggtcattga agcgaacaat gtcgaagacg ctgttgataa agtacaagat
     2281 attatagaac caaatgatgt tgtattattg tcacctgctt gtgcgagttg ggatcaatat
     2341 agtacttttg aagagcgtgg agagaaattt attgaaagat tccgtgccca tttaccatct
     2401 tattaaaggg tgtgagtatt gatggatgat aaaacgaaga acgatcaaca agaatcaaat
     2461 gaagataaag atgaattaga attatttacg aggaatacat ctaagaaaag acggcaaaga
     2521 aaaaggtcaa aggctacaca tttttctaat caaaataaag atgatacatc tcaacaagct
     2581 gattttgatg aagaaattta cttgataaat aaagacttca aaaaagaaga aagcaatgat
     2641 gaaaataatg gttctgcttc tagtcatgcg aatgataata atatcgatga ttctacagac
     2701 tctaatattg aaaatgagga ttatagatat aatcaagaaa ttgacgacca aaatgaatcg
     2761 aatggaattg cagtcggcaa cgaacaacct caatcagctc ctaaagaaca aaatagcgac
     2821 tcgaatgatg aggaaacagt aacgaaaaaa gaacgaaaaa gtaaagtaac acaattaaag
     2881 ccattaacac ttgaagaaaa gcggaagtta agacgtaagc gacaaaaacg aatccaatac
     2941 agtgttatta caatattagt attgttgatt gctgttatat taatttacat gttttcacca
     3001 cttagtaaaa ttgcgcatgt aaatataaat ggaaataatc acgttagtac ttcaaagata
     3061 aacaaagttt taggtgttaa aaatgattcg aggatgtata cgtttagtaa aaaaaatgct
     3121 attaatgatc tcgaagagga tccattaatc aaaagtgttg agatacacaa gcaattacca
     3181 aacacattaa acgtagatat cacagaaaat gaaattattg ctttagtgaa atataaaggt
     3241 aaatatttac ctttattaga aaatggtaaa ttgcttaaag gttcaaatga tgtcaaaatt
     3301 aatgatgcac ctgtcatgga tggtttcaaa ggtacaaaag aagatgatat gattaaggcg
     3361 ttatctgaaa tgacacctga agttagacga tatattgccg aagtgacata cgccccaagt
     3421 aaaaacaaac aaagcagaat tgaattgttt acgacagatg gacttcaagt aatcggtgat
     3481 atttcgacga tatctaagaa aatgaaatat tatccgcaga tgtcacaatc attatcaagg
     3541 gatagttcgg gtaaactaaa aacaagaggc tatattgatt tatcagtcgg tgcttcattt
     3601 atcccatacc gtggaaacac gtctagtcaa tcagaaagcg ataaaaatgt gactaaatca
     3661 tctcaagagg aaaatcaagc aaaagaagaa ttacaaagcg ttttaaacaa aattaacaaa
     3721 caatcaagta agaataatta aaaaattttg atattgtcta tgtttatagt tcacaagcca
     3781 ttcaacgtat tgtaaactaa ggatagtgta tttttttaat agtaatttgt caggaggtgc
     3841 ctatctatgg aagaacatta ctacgtaagt attgatattg gatcatcaag cgtaaaaaca
     3901 atagtaggcg agaaatttca caatggtata aatgtgatag gtacaggaca aacctacacg
     3961 agcggtataa aaaatggttt aattgatgat tttgatattg cgcgacaagc aatcaaagac
     4021 acaattaaaa aggcatcaat cgcttcgggt gttgatatta aagaagtttt cctgaaatta
     4081 cctatcattg gaacggaagt ttatgatgaa tcaaatgaaa tcgactttta tgaggataca
     4141 gaaatcaacg gttcacatat cgaaaaagta ttagaaggta ttagagaaaa aaatgatgtg
     4201 caagaaacag aagtaattaa tgtgttcccg attcgtttta tagtcgataa agaaaatgag
     4261 gtttcagacc ctaaagaatt aattgccaga cattcattaa aggttgaagc aggcgtaatt
     4321 gctattcaaa aatcgatttt aattaatatg attaaatgcg tagaagcatg tggtgttgat
     4381 gtattagatg tttactctga tgcatataac tatggttcaa tcctaacagc tactgaaaaa
     4441 gagttaggtg catgtgtcat tgatattggt gaagacgtta cgcaagttgc tttttatgaa
     4501 cgcggtgaat tagtagatgc tgattctatc gaaatggcag ggcgtgatat tacagacgat
     4561 attgcacaag gattaaacac ttcttatgaa actgctgaaa aagttaaaca ccaatatggt
     4621 catgcattct atgattctgc ttcagatcaa gatatcttca ctgttgaaca ggttgatagt
     4681 gatgaaacag tacagtatac tcaaaaagat ttgagtgact ttattgaagc gcgtgtagaa
     4741 gaaatattct tcgaagtatt tgatgtttta caagatttag gattaacaaa agtaaatggt
     4801 gggtttattg taactggtgg atctgcaaac ttacttggcg taaaagaatt attatcagat
     4861 atggtaagtg aaaaagttag aattcacacg ccatcacaaa tgggaattag aaaacctgaa
     4921 ttttcttcag caatttctac aatttctagt agtatcgctt ttgatgagtt attagattat
     4981 gttacaatta attatcatga taatgaagaa actgaagaag atgttattga tgtgaaagac
     5041 aaagataacg aatctaaatt aggcggattt gattggttta aacgtaaaac aaacaaaaaa
     5101 gatactcatg aaaatgaagt agagtcaaca gatgaagaaa tttatcaatc agaagataat
     5161 catcaggaac ataaacagaa tcatgaacat gttcaagaca aagataaaga taaagaagaa
     5221 agtaaattca aaaaactaat gaaatctcta tttgaatgat tattggccaa taaaactagg
     5281 aggaaattta aatgttagaa tttgaacaag gatttaatca tttagcgact ttaaaggtca
     5341 ttggtgtagg tggtggcggt aacaacgccg taaaccgaat gattgaccac ggaatgaata
     5401 atgttgaatt tatcgctatc aacacagacg gtcaagcttt aaacttatct aaagctgaat
     5461 ctaaaatcca aatcggtgaa aaattaacac gtggtttagg agcaggagct aaccctgaaa
     5521 tcggtaaaaa agctgcagag gaatctcgtg aacaaattga agatgcaatc caaggtgcag
     5581 acatggtatt tgttacttct ggtatgggtg gcggaactgg tactggtgca gcaccagtcg
     5641 ttgctaaaat tgcaaaagaa atgggcgcat taactgttgg tgttgtaact cgtccattta
     5701 gttttgaagg acgtaaacgt caaactcaag ctgctgctgg agtagaagct atgaaagctg
     5761 cagtagatac attaatcgtt ataccaaatg accgtttatt agatatcgtt gacaaatcta
     5821 cgccaatgat ggaagcattt aaagaagctg ataacgtgtt acgccaaggt gtacaaggta
     5881 tctcagactt aatcgctgtt tctggtgaag taaacttaga ctttgcagac gttaagacaa
     5941 ttatgtctaa ccaaggttct gcattaatgg gtattggtgt ttcttctggt gaaaatagag
     6001 cggtagaagc tgctaaaaaa gcaatctctt ctccattact tgaaacatct atcgttggtg
     6061 cacaaggtgt gcttatgaat attactggtg gcgagtcatt gtcattattt gaagcacaag
     6121 aggctgctga tattgtccaa gatgctgcag atgaagacgt taatatgatt ttcggtacag
     6181 ttattaatcc tgaattacaa gatgagattg ttgtaacagt tattgcaact ggttttgatg
     6241 acaaaccaac atcacatggt cgtaaatctg gtagcactgg attcggaaca agcgtaaata
     6301 cttctagcaa tgcaacttct aaagatgaat cattcacttc aaattcatca aatgcacaag
     6361 caactgatag tgtaagtgaa agaacgcata caactaaaga agatgatatt ccaagcttca
     6421 ttagaaatag agaagaaaga cgttcaagaa gaacaagacg tt
//
LOCUS       DAHHSC010000138         6334 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.139, whole genome shotgun sequence.
ACCESSION   DAHHSC010000138 DAHHSC010000000
VERSION     DAHHSC010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 6334)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6334)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6334
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.139"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            <1..1227
                     /locus_tag="NBI57_001958"
     CDS             <1..1227
                     /locus_tag="NBI57_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HCD1822892.1"
                     /translation="SLRDEALEMHKRNQGKLEVKPNVKVTNKEELSLAYSPGVAEPCK
                     DIYEDKRKVYDYTIKGNTVAVITDGTAVLGLGNIGPEASIPVMEGKAVLFKSFAGING
                     VPIALNTTDTEEIIKTVKLLEPNYGGINLEDISAPRCFEIEERLKKETNIPVFHDDQH
                     GTAIVTMAGLVNALRVVNKDIAKIKVVLNGAGAAGIAIVKLLYAYGVRNMVMCDSRGA
                     IFEGRSYGMNPTKDVVAKWTNKDKIEGSLEEVVKDADVFIGVSVANALSQDMVKSMAD
                     NPIIFAMANPNPEIIPDDAKAAGARVVGTGRSDYPNQINNVLAFPGIFRGALKVEATH
                     INEEMKKAAVEAIADLIDSSELNEDYCIPGPFDKRVAPSVARNVAKAAMESGVARIEV
                     DPQDVYDKTMKLTDLQ"
     gene            1422..2279
                     /locus_tag="NBI57_001959"
     CDS             1422..2279
                     /locus_tag="NBI57_001959"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HCD1822893.1"
                     /translation="MFKDFFNRTKKKKYLTVQDSKNNDVPAGIMTKCPKCKKIMYTKE
                     LAENLNVCFNCDHHIALTAYKRIEAISDEGSFTEFDKGMTSANPLDFPSYLEKIEKDQ
                     QKTGLKEAVVTGTAQLDGMKFGVAVMDSRFRMGSMGSVIGEKICRIIDYCTENRLPFI
                     LFSASGGARMQEGIISLMQMGKTSVSLKRHSDAGLLYISYLTHPTTGGVSASFASVGD
                     INLSEPKALIGFAGRRVIEQTINEKLPDDFQTAEFLLEHGQLDKVVHRNDMRQTLSEI
                     LKIHQEVTK"
     gene            2279..3223
                     /locus_tag="NBI57_001960"
     CDS             2279..3223
                     /locus_tag="NBI57_001960"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HCD1822894.1"
                     /translation="MLDFEKPLFEIRNKIESLKESQDKNDVDLQEEIDMLEASLERET
                     KKIYTNLKPWDRVQIARLQERPTTLDYIPYIFDSFMELHGDRNFRDDPAMIGGIGFLN
                     GRAVTVIGQQRGKDTKDNIYRNFGMAHPEGYRKALRLMKQAEKFNRPIFTFIDTKGAY
                     PGKAAEERGQSESIATNLIEMASLKVPVIAIVIGEGGSGGALGIGIANKVLMLENSTY
                     SVISPEGAAALLWKDSNLAKIAAETMKITAHDIKQLGIIDDVISEPLGGAHKDVEQQA
                     LAIKSAFVAQLDSLESLSRDEIANDRFEKFRNIGSYIE"
     gene            3492..4460
                     /gene="pfkA"
                     /locus_tag="NBI57_001961"
     CDS             3492..4460
                     /gene="pfkA"
                     /locus_tag="NBI57_001961"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002462395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HCD1822895.1"
                     /translation="MKKIAVLTSGGDSPGMNAAVRAVVRTAIYNEIEVYGVYHGYQGL
                     LNDDIHKLELGSVGDTIQRGGTFLYSARCPEFKEQEVRKVAIENLRKRGIEGLVVIGG
                     DGSYRGAQRISEECKEIQTIGIPGTIDNDINGTDFTIGFDTALNTIIGLVDKIRDTAS
                     SHARTFIIEAMGRDCGDLALWAGLSVGAETIVVPEVKTDIKEIADKIEQGIKRGKKHS
                     IVLVAEGCMTAQDCQKELSQYINVDNRVSVLGHVQRGGSPTGADRVLASRLGGYAVDL
                     LMQGETAKGVGIKNNKIVATSFDEIFDGKDHKFDYSLYELANKLSI"
     gene            4482..6239
                     /gene="pyk"
                     /locus_tag="NBI57_001962"
     CDS             4482..6239
                     /gene="pyk"
                     /locus_tag="NBI57_001962"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HCD1822896.1"
                     /translation="MRKTKIVCTIGPASESEEMIEKLINAGMNVARLNFSHGSHEEHK
                     GRIDTIRKVAKRLDKIVAILLDTKGPEIRTHNMKDGIIELERGNEVIVSMNEVEGTPE
                     KFSVTYENLINDVQVGSYILLDDGLIELQVKDIDHAKKEVKCDILNSGELKNKKGVNL
                     PGVRVSLPGITEKDAEDIRFGIKENVDFIAASFVRRPSDVLEIREILEEQKANISVFP
                     KIENQEGIDNIAEILEVSDGLMVARGDMGVEIPPEKVPMVQKDLIRQCNKLGKPVITA
                     TQMLDSMQRNPRATRAEASDVANAIYDGTDAVMLSGETAAGLYPEEAVKTMRNIAVSA
                     EAAQDYKKLLSDRTKLVETSLVNAIGISVAHTALNLNVKAIVAATESGSTARTISKYR
                     PHSDIIAVTPSEETARQCSIVWGVQPVVKKGRKSTDALLNNAVATAVETGRVSNGDLI
                     IITAGVPTGETGTTNMMKIHLVGDEIANGQGIGRGSVVGTTLVAETVKDLEGKDLSDK
                     VIVTNSIDETFVPYVEKALGLITEENGITSPSAIVGLEKGIPTVVGVEKAVKNISNNM
                     LVTIDAAQGKIFEGYANVL"
BASE COUNT         2242 a          853 c         1316 g         1923 t
ORIGIN      
        1 tcattaagag atgaagcatt ggaaatgcac aaacgtaatc aaggtaaatt agaagttaaa
       61 ccaaatgtaa aagttactaa taaagaggaa ttaagtttag catactcacc tggcgttgct
      121 gaaccgtgta aagatattta tgaagataaa agaaaagtat atgattacac aattaaagga
      181 aatacagttg cagttattac tgatggaaca gcggtattag gtttaggtaa cattggacct
      241 gaagcaagta ttcctgtaat ggaaggtaaa gcagtattat tcaaaagctt cgctggtatc
      301 aatggggtgc ctattgcgtt aaatacaact gataccgaag aaatcattaa aacagttaag
      361 ttgttagaac ctaattatgg tggtattaat ttagaggata tttcggcacc acgttgtttt
      421 gaaattgaag aacgattgaa aaaagaaact aatattccgg tattccatga cgatcaacat
      481 ggtacagcaa ttgtaacaat ggcaggtttg gtaaatgcat tgagagttgt taacaaagat
      541 attgctaaaa taaaagttgt actaaatggt gctggtgcag caggaatagc cattgttaaa
      601 ttactatacg cgtatggtgt aagaaatatg gttatgtgtg actcaagagg cgcaattttt
      661 gaaggacgtt catatggtat gaatcctacg aaagatgttg tagctaaatg gacaaataaa
      721 gataagattg aagggtcttt agaagaagtc gtaaaagacg cagatgtatt tatcggagtt
      781 tctgtagcta atgcgctgtc acaagatatg gttaagagta tggcagataa tccaattata
      841 tttgcaatgg ctaatccaaa tcctgaaata atacctgatg atgccaaagc ggcaggtgca
      901 cgagttgttg gtacaggacg ttcagactat cctaaccaaa ttaataatgt attagctttc
      961 cctggtattt ttagaggtgc attaaaggtt gaagctacac atataaatga agaaatgaaa
     1021 aaggcagctg tagaagcgat tgctgattta atcgatagtt ctgaattaaa tgaagactac
     1081 tgtatcccag gaccgtttga taaacgtgta gcgccatcag ttgctcgtaa tgttgctaaa
     1141 gcggcaatgg aatctggagt agctaggatt gaagttgatc cgcaagatgt gtatgataaa
     1201 acaatgaaac ttacagattt acaataaaaa taattaacgt ttaattaaat tgcttaaaag
     1261 ttagcaataa agagtcatta aaatgatgaa aatgaaagtt tatgataaac attcaacagt
     1321 caaacgaata taaatcaaat aaatttaaac ccgtttttaa ctggtcaagt tcagtttaag
     1381 gctctaaatg gttagaacag aggttatttg gaggttttcc tatgtttaaa gattttttta
     1441 atcgaacaaa gaaaaagaaa tatcttacag tacaagactc taaaaataat gatgtgcctg
     1501 caggtattat gactaagtgt ccaaagtgta agaaaattat gtacacaaaa gaattagctg
     1561 aaaatttaaa tgtgtgcttt aattgtgatc atcatattgc tttaactgcg tataaacgta
     1621 tagaagcaat ttctgatgaa ggatcattta cagaattcga taagggaatg acctctgcga
     1681 atccattaga ttttccaagt tatttagaaa aaattgaaaa ggaccaacaa aagacaggtc
     1741 ttaaagaagc agttgtgact ggtacagcgc aactagatgg tatgaaattt ggcgttgctg
     1801 tcatggattc acgttttaga atgggaagta tgggatcggt tatcggtgaa aagatatgtc
     1861 gcatcattga ttactgcact gagaaccgtt taccatttat tcttttctct gcaagtggtg
     1921 gtgcacgtat gcaagaaggt attatttcct tgatgcaaat gggtaaaacc agtgtatctt
     1981 taaaacgtca ttctgacgct ggactattat atatatcata tttaacacat ccaactactg
     2041 gtggtgtatc tgcaagtttt gcatcagttg gtgatataaa tttaagtgag ccaaaagcgt
     2101 tgataggttt tgcaggtcgt cgagttattg aacagacaat aaacgaaaaa ttgccagatg
     2161 atttccaaac tgcagaattt ttattagagc atggacaatt ggataaagtt gtacatcgta
     2221 atgatatgcg tcaaacattg tctgaaattc taaaaatcca tcaagaggtg actaaataat
     2281 gttagatttt gaaaaaccac tttttgaaat tcgaaataaa attgaatctt taaaagaatc
     2341 tcaagataaa aatgatgtgg atttacaaga agaaattgac atgcttgaag cgtcattgga
     2401 acgagaaact aaaaaaatat atacaaatct aaaaccatgg gatcgtgtgc aaattgcgcg
     2461 tttgcaagaa agacctacga ccctagatta tattccatat atctttgatt cgtttatgga
     2521 actacatggt gatcgtaatt ttagagatga tccagcaatg attggtggta ttggcttttt
     2581 aaatggtcgt gctgttacag ttattggaca acaacgtgga aaagatacaa aagataatat
     2641 ttatcgaaat tttggtatgg cgcatccaga aggttatcga aaagcattac gtttaatgaa
     2701 acaagctgaa aaattcaatc gtcctatctt tacatttata gatacaaaag gtgcatatcc
     2761 tggtaaagct gctgaagaac gtggacaaag tgaatctatc gcaacaaatt tgattgagat
     2821 ggcttcatta aaagtaccag ttattgcgat agtcattggt gaaggtggca gtggaggtgc
     2881 tctaggtatt ggtattgcca ataaagtatt gatgttagag aatagtactt actctgttat
     2941 atctcctgaa ggtgcagcgg cattattatg gaaagacagt aatttggcta aaattgcagc
     3001 tgaaacaatg aaaattactg cccatgatat taagcaatta ggtattatag atgatgtcat
     3061 ttctgaacca cttggcggtg cacataaaga tgttgaacag caagctttag ctattaaatc
     3121 agcgtttgtt gcacagttag attcacttga gtcattatca cgtgatgaaa ttgctaatga
     3181 tcgctttgaa aaattcagaa atatcggttc ttatatagaa taatcaactt gagcattttt
     3241 atgttaaatc gatactgggt tttaccataa attgaagtac attaaaacaa taatttaata
     3301 tttagatact gaattttaac taagattagt agtcaaaatt gtggctacta atcttttttt
     3361 aattaagtta aaataaaatt caatatttaa aacgtttaca tcaattcaat acattagttt
     3421 tgatggaatg acatatcaat ttgtggtaat ttagagttaa agataaatca gttatagaaa
     3481 ggtatgtcgt catgaagaaa attgcagttt taactagtgg tggagattca cctggaatga
     3541 atgctgccgt aagagcagtt gttcgtacag caatttacaa tgaaattgaa gtttatggtg
     3601 tgtatcatgg ttaccaagga ttgttaaatg atgatattca taaacttgaa ttaggatcag
     3661 ttggggatac gattcagcgt ggaggtacat tcttgtattc agcaagatgt ccagagttta
     3721 aggagcaaga agtacgtaaa gttgcaatcg aaaacttacg taaaagaggg attgagggcc
     3781 ttgtagttat tggtggtgac ggtagttatc gcggtgcaca acgcatcagt gaggaatgca
     3841 aagaaattca aactatcggt attcctggta cgattgacaa tgatatcaat ggtactgatt
     3901 ttacaattgg atttgacaca gctttaaata cgattattgg cttagtcgac aaaattagag
     3961 atactgcgtc aagtcacgca cgaacattta tcattgaagc aatgggccgt gattgtggag
     4021 atctagcatt atgggctgga ttatcagttg gtgctgagac aattgtagtt ccagaagtga
     4081 aaacagatat taaagaaata gctgataaaa ttgaacaagg tattaaacgt ggtaagaaac
     4141 actcaatcgt tcttgtagca gaaggttgca tgactgcgca agattgtcaa aaagaattat
     4201 cacaatacat caatgttgat aatagagtgt ctgtgttagg tcacgttcaa cgtggtggta
     4261 gcccaacagg tgcggataga gttttagcat cacgtttagg tggatatgcg gtagacttat
     4321 taatgcaagg tgaaacagct aagggtgttg gaattaagaa caataaaatt gtagcaacat
     4381 cttttgatga aatttttgat ggtaaagatc ataaatttga ttatagtcta tatgaacttg
     4441 ctaacaagtt atctatataa gatttcagga ggaattataa aatgagaaaa actaaaattg
     4501 tatgtacaat tggaccagct tcagaatcag aagaaatgat tgagaaatta atcaatgctg
     4561 gtatgaacgt tgcacgatta aacttttcac atggtagtca tgaagagcat aaaggtagaa
     4621 ttgatacaat tcgtaaagta gctaaaagat tagacaaaat tgtagcaatt ttattagata
     4681 cgaaaggtcc agaaattcgt acgcataata tgaaagacgg tatcattgaa cttgaacgtg
     4741 gtaacgaagt tattgttagc atgaatgaag ttgaaggaac acctgaaaag ttctcagtaa
     4801 catatgaaaa cttaattaac gatgttcaag taggttcata cattttactt gatgatggct
     4861 taattgaatt acaagttaaa gatattgacc atgctaaaaa agaagttaaa tgtgatattt
     4921 taaactctgg tgagcttaaa aacaaaaaag gtgttaactt acctggtgta agagtaagtt
     4981 tacctggtat tacagaaaaa gatgctgaag atatccgttt cggtattaaa gaaaatgttg
     5041 acttcattgc agcaagtttc gtacgtcgtc ctagtgatgt tttagaaatt cgtgaaattt
     5101 tagaagaaca aaaagctaac atttcagtat tccctaaaat tgaaaaccaa gaaggtattg
     5161 ataatattgc ggaaattctt gaagtgtctg atggtttaat ggttgcacgt ggtgacatgg
     5221 gtgttgaaat tccacctgaa aaagtaccaa tggttcaaaa agatttaatc agacaatgta
     5281 acaaattagg taaaccagtt attacagcta cacaaatgtt agattctatg caacgtaacc
     5341 cacgtgctac acgtgcagaa gctagtgacg ttgccaacgc aatctatgat ggtacagatg
     5401 cagtaatgtt atctggtgaa actgctgctg gtttatatcc tgaagaagct gttaaaacaa
     5461 tgagaaatat tgctgtatca gctgaagcag cccaagatta taaaaagtta ttgtcagatc
     5521 gtactaaatt agttgaaact tcattagtga atgctatcgg tatttcggtt gcacatacag
     5581 ctttaaactt aaatgttaaa gcaattgtag ctgctactga aagtggttca acggcacgta
     5641 ctatctctaa atatcgtcca cattcagaca ttattgcggt gactccaagt gaagaaacag
     5701 cacgtcaatg ttcaattgtt tggggagttc aacctgtagt taaaaaagga cgtaagagta
     5761 cagatgcatt gttaaacaat gcagttgcaa cagctgttga aactggtaga gtatctaatg
     5821 gtgatttaat cattattact gctggtgtac caactggtga aactggaact actaatatga
     5881 tgaaaatcca cctagttggt gacgaaattg ctaatggtca aggtattgga cgtggatcag
     5941 ttgttggtac tacgttagtt gctgaaactg ttaaagattt agaaggtaaa gatttatctg
     6001 acaaagttat cgttactaac tcaatcgatg aaacgtttgt accttatgta gaaaaagctt
     6061 taggattaat tacagaagaa aatggtatta catcaccaag tgcaattgtt ggtttagaaa
     6121 aaggtattcc aacagttgta ggtgtagaaa aagctgttaa aaacataagc aataacatgt
     6181 tagttacgat tgatgctgct caaggtaaaa tctttgaagg atatgcaaac gtactataat
     6241 ttataaaaaa acgtctttcc atttatcaac aatggaaagg cgttttttgg ttcatctggt
     6301 attttatgac gtaattaata ggttatttga taat
//
LOCUS       DAHHSC010000139         6275 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.140, whole genome shotgun sequence.
ACCESSION   DAHHSC010000139 DAHHSC010000000
VERSION     DAHHSC010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 6275)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6275)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6275
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.140"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            8..322
                     /locus_tag="NBI57_001963"
     CDS             8..322
                     /locus_tag="NBI57_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1822897.1"
                     /translation="MYFVLAIFTIISASVSLGYSIQACASSHNINAYYALSRSLPLFL
                     LAIFSLVIHSAIFLITISIAMILVQFLDAIVGYKSEDVFKTYGPLATSVVNLILLIVF
                     LF"
     gene            complement(328..467)
                     /locus_tag="NBI57_001964"
                     /pseudo
     CDS             complement(328..467)
                     /locus_tag="NBI57_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499406.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(654..2051)
                     /gene="sufB"
                     /locus_tag="NBI57_001965"
     CDS             complement(654..2051)
                     /gene="sufB"
                     /locus_tag="NBI57_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019469760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HCD1822898.1"
                     /translation="MAKKAPDVGDYKYGFHDDDVSIFRSERGLTENIVREISNMKNEP
                     EWMLDFRLKSLKLFYKMPMPQWGGDLSELNFDDITYYVKPSEQAERSWDEVPEEIKRT
                     FDKLGIPEAEQKYLAGVSAQYESEVVYHNMEKELEEKGIIFKDTDSALQENEELFKKY
                     FASVVPAADNKFAALNSAVWSGGSFIYVPKNIKLDTPLQAYFRINSENMGQFERTLII
                     ADEGASVHYVEGCTAPVYTTSSLHSAVVEIIVHKDAHVRYTTIQNWANNVYNLVTKRT
                     FVYENGNMEWVDGNLGSKLTMKYPNCVLLGEGAKGSTLSIAFAGKGQVQDAGAKMIHK
                     APNTSSTIVSKSISKNGGKVIYRGIVHFGRKAKGARSNIECDTLILDNESTSDTIPYN
                     EVFNDQISLEHEAKVSKVSEEQLFYLMSRGISEEEATEMIVMGFIEPFTKELPMEYAV
                     EMNRLIKFEMEGSIG"
     gene            complement(2202..2666)
                     /locus_tag="NBI57_001966"
     CDS             complement(2202..2666)
                     /locus_tag="NBI57_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HCD1822899.1"
                     /translation="MNFNNLDQLYRSVIMDHYKNPRNKGVLDNGSMTVDMNNPTCGDR
                     IRLTFDIEDGIIKDAKFEGEGCSISMASASMMTQAVKGHSLGEAMQMSQEFTKMMLGE
                     DYVITEEMGDIEALQGVSQFPARIKCATLAWKALEKGTVAKEGKAEGTTEEE"
     gene            complement(2656..3906)
                     /locus_tag="NBI57_001967"
     CDS             complement(2656..3906)
                     /locus_tag="NBI57_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HCD1822900.1"
                     /translation="MNEVAEHSFDVNEVIKDFPILDQKVNGKRLAYLDSTATSQTPMQ
                     VLNVLEDYYKRYNSNVHRGVHTLGSLATDGYENARETVRRFINAKYFEEIIFTRGTTA
                     SINLVAHSYGDANVEEGDEIVVTEMEHHANIVPWQQLAKRKNATLKFIPMTADGELNI
                     EDIKQTINDKTKIVAIAHISNVLGTINDVKTIAEIAHQHGAIISVDGAQAAPHMKLDM
                     QEMNADFYSFSGHKMLGPTGIGVLFGKRELLQKMEPIEFGGDMIDFVSKYDATWADLP
                     TKFEAGTPLIAQAIGLAEAIRYLERIGFDAIHKYEQELTIYAYEQMSAIEGIEIYGPP
                     KDRRAGVITFNLQDVHPHDVATAVDTEGVAVRAGHHCAQPLMKWLNVSSTARASFYIY
                     NTKEDIDQLINALKQTKEFFSYEF"
     gene            complement(4012..5319)
                     /gene="sufD"
                     /locus_tag="NBI57_001968"
     CDS             complement(4012..5319)
                     /gene="sufD"
                     /locus_tag="NBI57_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HCD1822901.1"
                     /translation="MTTDILNISEEQLVDYSKAHNEPSWMTELRKKALKLTETLEMPK
                     PDKTKLRKWDFDSFKQHDVKGDVYQSLSQLPESVREIIDVDHSKNLVIQHNNTIAYTQ
                     VDDNASKDGVIVEGLADALMNHSDLVQKHFMKDAVTVDEHRITALHTALVNGGVFVYV
                     PKNVVVEHPVQYVVLHDDKNASFYNHVIIVTEESAEVTYVENYLSNASGEGNQLNIIS
                     EVIAGANSNITYGSVDYMDKGFTGHIIRRGITEADASINWALGLMNEGSQIIDNTTNL
                     FGDRSTSSLKSVVVGTGEQKINLTSKIVQYGKETDGYILKHGVMKEHASSVFNGIGYI
                     KHGGTKSIANQESRVLMLSEHARGDANPILLIDEDDVQAGHAASVGRVDPDQLYYLMS
                     RGISQREAERLVIHGFLDPVVRELPIEDVKRQLREVIERKVSK"
     gene            complement(5417..6178)
                     /gene="sufC"
                     /locus_tag="NBI57_001969"
     CDS             complement(5417..6178)
                     /gene="sufC"
                     /locus_tag="NBI57_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HCD1822902.1"
                     /translation="MASTLEIKDLHVSIEDKEILKGVNLTINTDEIHAIMGPNGTGKS
                     TLSSAIMGHPSYEVTKGEVLLDGVNILELEVDERAKAGLFLAMQYPSEITGVTNADFM
                     RSAINAKREEGQEINLMQFIKKLDKNMDFLDIDKDMAQRYLNEGFSGGEKKRNEILQL
                     MMLEPKFAILDEIDSGLDIDALKVVSKGINQMRGENFGALMITHYQRLLNYITPDKVH
                     VMYAGKVVKSGGPELAKRLEEEGYEWVKEEFGSAE"
BASE COUNT         1905 a         1215 c          892 g         2263 t
ORIGIN      
        1 gattaacatg tattttgttt tagcaatatt tacaatcatt agtgccagtg taagtttagg
       61 ttattcaatt caagcatgtg catctagtca taatataaat gcatattatg cacttagtcg
      121 aagcttacct ttatttttat tagctatttt ttctttagtc attcatagtg ctatattttt
      181 gataactata tccattgcaa tgattttagt tcaattttta gatgcgattg ttggttataa
      241 aagtgaagat gtctttaaaa cttatggtcc attagcaaca tctgtagtga acttaatatt
      301 attaatagtt ttcttatttt aactcactta tacaacgaat cttaatcgaa ctaatatcga
      361 attatcttta aagaatattt gatgtagttt tcaattaatt taataaagac cagcactcta
      421 atgccacaat catattgtat ttgtgttgtc gctttatcca ccatcaatga ttatttttta
      481 caccaatcaa aaaatcggac tgatataaat aagtacaaag cttatctatc aatccgattt
      541 agttataaaa caaaaaaagc cacagtaatg tggctttttg ttatattcag tatcaaaatg
      601 gtatcaatac ccatttccgg aagtcaagaa tggcttaaca acgcggttta aagctatcca
      661 atactacctt ccatttcgaa cttgattaaa cggttcattt cgaccgcgta ttccattgga
      721 agttcttttg taaatggttc gatgaatccc ataacaatca tttctgtcgc ttcttcttca
      781 gaaataccac gactcattag ataaaataat tgttcttcag aaacttttga aaccttggct
      841 tcatgttcta atgatatttg atcgttgaat acttcgttat atggaattgt atctgatgtt
      901 gattcgttat ctaagattaa tgtatcacat tcaatatttg aacgagcacc ttttgcttta
      961 cgtccaaaat gaacaatacc gcgataaata actttaccac catttttaga aatagattta
     1021 gaaacaatcg ttgaagatgt atttggcgct ttatgaatca ttttagcacc ggcatcttga
     1081 acttgtcctt taccagcaaa tgcaatagat aatgtactac cttttgcacc ttcacctaaa
     1141 agaacacagt ttggatattt catcgttaac ttagaaccta agttaccatc tacccattcc
     1201 atatttccgt tttcataaac aaaagtacgt tttgtaacta aattgtatac attgttcgcc
     1261 cagttttgaa tcgtagtata acgaacgtgc gcatctttat gcacaatgat ttccacaaca
     1321 gcagagtgta aagaactagt tgtataaact ggtgcagtac aaccttctac gtaatgtaca
     1381 gaagcacctt catcagcaat gattaatgta cgttcaaatt gacccatgtt ctcagagtta
     1441 atacggaaat aagcttgtag tggcgtatct agtttgatat ttttaggtac ataaatgaac
     1501 gaaccacctg accatactgc tgagtttaac gccgcaaatt tgttatctgc tgcaggtact
     1561 acagaagcaa agtatttttt gaataattct tcattttctt gtaaagcact atctgtatct
     1621 ttaaagataa tacctttttc ttcaagttct ttttccatat tatggtaaac aacttcagat
     1681 tcatattgag cagaaacacc agctaaatat ttttgttcag cttcaggaat tcctaattta
     1741 tcgaaagttc ttttaatttc ttctggcact tcatcccatg aacgttcagc ttgttctgaa
     1801 ggctttacat agtaagtaat gtcatcgaaa ttcaattctg ataagtcgcc accccattga
     1861 ggcattggca ttttataaaa caattttaat gatttaagac ggaaatctaa catccattcc
     1921 ggctcatttt tcatgttaga aatttctcta acgatattct cagttaaacc acgttctgat
     1981 ctgaaaatgg atacatcatc gtcgtggaat ccatatttat aatccccaac atcaggtgct
     2041 tttttagcca tttcaatcac tccttaaaaa tatatttaca acggcgtgcg taagttttta
     2101 attcatgatg taaaccatat tataacaatg acatgacatc ttataaaaat ttttatactt
     2161 ttatatgtct aatatcaaaa tcatctatga ttaacagcat tctattcttc ttcagtcgta
     2221 ccttctgctt taccttcttt agcaacagta cctttttcca atgctttcca agctaatgtg
     2281 gcacatttaa tacgagctgg gaattgagat acaccttgca atgcttcaat atctcccatt
     2341 tcttctgtaa tcacatagtc ttcaccaagc atcattttcg taaattcttg gctcatttgc
     2401 attgcttctc caagtgaatg acctttaaca gcttgtgtca tcatcgatgc acttgccatt
     2461 gaaatcgaac aaccttcacc ttcaaactta gcatctttta taatgccgtc ttctatatca
     2521 aatgttagtc gtatacggtc accgcatgtc gggttattca tatctactgt catagacccg
     2581 ttatctaata cacctttatt tctaggattt ttataatgat ccataatgac agatctatat
     2641 aattgatcta gattattaaa attcataaga gaaaaactcc ttcgtttgtt tcaaggcatt
     2701 tattaactga tcaatgtctt ctttcgtgtt gtatatataa aaactcgctc tagctgttga
     2761 agacacattt aaccatttca ttaacggttg cgcacaatga tgcccagctc taaccgctac
     2821 accttctgta tctacggctg tagcaacatc gtgtggatgt acatcttgta aattaaacgt
     2881 tattacacct gcacgacgat ccttaggcgg gccataaatt tcaattcctt caattgcaga
     2941 catttgctca taagcatata tcgttaattc ttgttcatat ttatgaattg catcaaaacc
     3001 tatgcgttct aaatagcgaa tagcttctgc aagcccaatt gcttgagcaa ttaatggagt
     3061 acccgcctca aatttagtag gtaaatcagc ccatgttgca tcatacttac ttacaaaatc
     3121 aatcatgtca ccaccgaact caatcggttc cattttttgt agtaactcac gtttaccaaa
     3181 taatacgcca atacctgttg gtccaagcat tttatgacca ctaaaactat aaaaatcagc
     3241 attcatttct tgcatatcaa gtttcatatg tggtgctgct tgcgccccat caacactgat
     3301 aattgcgcca tgttgatgag ctatttctgc aatggtttta acatcattaa ttgtaccgag
     3361 tacattagaa atatgtgcaa tagcaacgat ctttgtttta tcattaatcg tttgcttaat
     3421 atcttcgatg tttaattcac cgtcagctgt cattggtata aatttcaatg tcgcattttt
     3481 acgctttgct aactgttgcc aaggaacaat attggcatga tgctccattt cagtgacaac
     3541 aatttcatcg ccctcttcaa catttgcatc accatagcta tgtgctacaa ggttaatcga
     3601 cgcagttgtt ccgcgtgtga aaatgatttc ttcaaaatac ttcgcattaa taaaacgacg
     3661 aacggtttca cgggcatttt cataaccatc agttgccaat gatcctaatg tatgaacacc
     3721 acgatgaacg tttgaattat aacgcttgta gtaatcctct aaaacattta acacttgcat
     3781 aggcgtttga cttgtcgctg ttgaatcaag atatgctaaa cgtttgccat tgactttttg
     3841 atctaatatc ggaaaatcct tgattacttc attaacgtca aatgagtgtt cggccacttc
     3901 attcacgacc tttcttaaat aaaaatccta atcatttaaa tactgacgtt gtattagtct
     3961 tataccaata tcgacagtct atatctatta caaactttta ttttcaaaat attatttaga
     4021 aactttgcgt tcaattactt ctctcaattg acgtttaacg tcttcgatag gtaattcacg
     4081 tactactgga tctaagaaac catgtataac aagacgttcc gcttctcttt gagaaatacc
     4141 acgactcatt aaatagtaaa gttgatctgg atcaacacga cctactgatg cagcatgacc
     4201 agcttgtaca tcatcttcat caattaataa aataggattc gcgtcaccac gagcatgttc
     4261 agataacatt aatacacgtg attcctgatt agcaattgat ttagttccac catgcttaat
     4321 gtagccgata ccattaaata cagatgatgc atgttctttc ataacaccat gtttaaggat
     4381 ataaccatct gtttctttac catattgtac gattttagat gttagattaa ttttttgttc
     4441 tcctgtacct acaactactg atttaagtga acttgttgaa cgatcaccaa ataaatttgt
     4501 tgtattatca ataatttggc taccctcatt cattaaacct agtgcccaat taattgaggc
     4561 atccgcttca gtaataccac gtcgaatgat atgacctgta aagcctttat ccatatagtc
     4621 cactgagcca tatgtgatat ttgaatttgc accagcaatc acttcagaaa taatatttaa
     4681 ttgatttcct tcaccagatg catttgataa gtaattttca acatatgtga cttcggcgct
     4741 ttcttcagta acgatgataa catggttata aaagcttgca tttttgtcgt cgtgcaacac
     4801 aacgtattgt actggatgtt ctacaactac atttttagga acataaacaa atacgccacc
     4861 attaactaat gccgtgtgta gcgctgtgat acgatgttca tctactgtta ctgcatcttt
     4921 cataaagtgc ttttgtacta aatcactatg gttcataaga gcgtctgcta aaccttcaac
     4981 gataacgcca tctttcgatg cattatcatc aacttgtgtg tacgcaatcg tattattatg
     5041 ttgaattact aagtttttag aatgatctac gtcaataatt tctcttactg actcaggtaa
     5101 ttgtgataaa gattgataaa catcaccttt tacatcgtgt tgtttaaaag aatcaaaatc
     5161 ccattttctt aattttgttt tatcaggttt tggcatttct aaagtttctg ttaatttcaa
     5221 agctttttta cgtaattctg tcatccaaga aggttcattg tgggctttag aataatcaac
     5281 aagttgttct tcagaaatgt tcaaaatatc agttgtcata actttttcct cctacttgat
     5341 gaaatttact atttcgtata aatgttcatt ttcatcatat atagatgaac atctcatgga
     5401 tactgtatta ataagattat tcagctgaac cgaactcttc tttaacccat tcatatcctt
     5461 cttcttcaag acgttttgct aattctggac caccagattt aacgacttta ccagcataca
     5521 ttacatgtac tttatcagga gtaatgtaat ttaataatcg ttgatagtgt gtaatcatta
     5581 atgcaccaaa gttttcccca cgcatttggt taataccttt agatacaact tttaatgcat
     5641 cgatgtctaa ccctgaatcg atttcatcta agattgcaaa cttaggttct aacatcatta
     5701 attgtaagat ttcgttacgt ttcttctctc cacctgagaa accttcattt aaataacgtt
     5761 gtgccatgtc tttatctatg tctagaaaat ccatgttttt atctaatttc ttaataaatt
     5821 gcattaagtt gatttcttgt ccttcttcac gtttcgcatt gattgctgaa cgcatgaaat
     5881 cagcatttgt aacacctgta atttctgatg gatattgcat tgccaagaat aatcctgctt
     5941 ttgctctttc atcaacttct aattctaaaa tatttacacc gtctaaaagt acttctcctt
     6001 tagttacttc atagcttggg tgtcccataa ttgcagatga taaagttgat ttacctgtcc
     6061 cgtttggtcc cataatcgca tgtatttcat cagtgttaat tgtcaagtta acacctttta
     6121 agatttcttt atcctcaata gacacatgta ggtctttgat ttctaatgtt gatgccattt
     6181 tatattccct ccgtaaaata taaagttttc ttaactagtt tataataatt ttaatttgta
     6241 gtcaaaaaga ctttgtaata atgcgttcag ttaat
//
LOCUS       DAHHSC010000140         6118 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.141, whole genome shotgun sequence.
ACCESSION   DAHHSC010000140 DAHHSC010000000
VERSION     DAHHSC010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 6118)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6118)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6118
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.141"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            193..1149
                     /locus_tag="NBI57_001970"
     CDS             193..1149
                     /locus_tag="NBI57_001970"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HCD1822903.1"
                     /translation="MLNSFDAAYHSLCEEVLEIGNTRNDRTNTGTISKFGHQLRFDLS
                     KGFPLLTTKKVSFKLVATELLWFIKGDTNIQYLLKYNNNIWNEWAFENYIKSDEYNGP
                     DMTDFGHRALSDPEFNEQYKEQMKQFKQRILEDDTFAKQFGDLGNVYGKQWRDWVDKD
                     GNHFDQLKTVIEQIKHNPDSRRHIVSAWNPTEIDTMALPPCHTMFQFYVQDGKLSCQL
                     YQRSADIFLGVPFNIASYALLTHLIAKECGLEVGEFVHTFGDAHIYSNHIDAIQTQLA
                     RESFNPPTLKINSDKSIFDINYEDLEIVDYESHPAIKAPIAV"
     gene            1349..1828
                     /locus_tag="NBI57_001971"
     CDS             1349..1828
                     /locus_tag="NBI57_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001830952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HCD1822904.1"
                     /translation="MTLSILVAHDLQRVIGFENQLPWHLPNDLKHVKKLSTGHTLVMG
                     RKTFESIGKPLPNRRNVVLTSDTSFNVEGVDVIHSIEDIYQLPGHVFIFGGQTLFEEM
                     IDKVDDMYITVIEGKFRGDTFFPPYTFEDWEVASSVEGKLDEKNTIPHTFLHLIRKK"
     gene            1843..2682
                     /gene="fakB2"
                     /locus_tag="NBI57_001972"
     CDS             1843..2682
                     /gene="fakB2"
                     /locus_tag="NBI57_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid kinase binding subunit FakB2"
                     /protein_id="HCD1822905.1"
                     /translation="MTKQIIVTDSTSDLSKEYLEANNIHVIPLSLTIEGASYVDQVDI
                     TSEEFINHIENDEDVKTSQPAIGEFISAYEELGKDGSEIISIHLSSGLSGTYNTAYQA
                     SQMVDANVTVIDSKSISFGLGYQIQHLVELVKEGVSTSEIVKKLNHLRENIKLFVVIG
                     QLNQLIKGGRISKTKGLIGNLMKIKPIGTLDDGRLELVHNARTQNSSIQYLKKEIAEF
                     IGDHEIKSIGVAHANVIEYVDKLKKVFNEAFHVNNYDINVTTPVISAHTGQGAIGLVV
                     LKK"
     gene            2768..3301
                     /gene="msrA"
                     /locus_tag="NBI57_001973"
     CDS             2768..3301
                     /gene="msrA"
                     /locus_tag="NBI57_001973"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HCD1822906.1"
                     /translation="MTKEYATLAGGCFWCMVKPFTSYPGIKSVVSGYSGGHVDNPTYE
                     QVCTNKTGHVEAVQITFDPEVTSFENILDIYFKTFDPTDDQGQFFDRGESYQPVIFYH
                     DEHQKKAAEFKKQQLNEQGIFKKPVITPIKPYKNFYPAEDYHQDYYKKNPVHYYQYQR
                     GSGRKAFIESHWGNQNA"
     gene            3294..3722
                     /gene="msrB"
                     /locus_tag="NBI57_001974"
     CDS             3294..3722
                     /gene="msrB"
                     /locus_tag="NBI57_001974"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HCD1822907.1"
                     /translation="MLKKDKSELTDIEYIVTQENGTEPPFMNEYWNHFAKGIYVDKIS
                     GKPLFTSEEKFHSECGWPSFSKALDDDEIIELVDKSFGMVRTEVRSEESNSHLGHVFN
                     DGPKESGGLRYCINSAAIQFIPYEKLEELGYGDLISHFDK"
     gene            3734..4234
                     /locus_tag="NBI57_001975"
     CDS             3734..4234
                     /locus_tag="NBI57_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HCD1822908.1"
                     /translation="MFKKLFGKGKEVQKDIAIYAPLTGEYVKIEDIPDPVFAQKMMGE
                     GFGINPTEGEVVSPIAGRVDNVFPTKHAIGLKADNGLELLVHIGLDTVQLDGEGFEVL
                     VSSGDEVNVGDPLVRFNLEFINNNAKSVISPIIITNTDQAASINIYDENAVIKGETKV
                     IDVTMN"
     gene            4234..4455
                     /locus_tag="NBI57_001976"
     CDS             4234..4455
                     /locus_tag="NBI57_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HCD1822909.1"
                     /translation="MKNYSFYQFVMTVRGRHDDKGRLAEEIFDDLAFPKHDDDFNILS
                     DYIETHGDFTLPMSVFDDLYEEYTEWLKF"
     gene            4647..>6118
                     /locus_tag="NBI57_001977"
     CDS             4647..>6118
                     /locus_tag="NBI57_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HCD1822910.1"
                     /translation="MDDKQHTSSSDDERAEIATSNQDQETNSSKRVHLKRWQFISILI
                     GTTLITAVITVVAYIFINQKISGLNKTDQANLNKIENVYKILNSDYYKKQDSDKLSKA
                     AIDGMVKELKDPYSEYLTKEQTKSFNEGVSGDFVGIGAEMQKKNDQIMVTSPMKGSPA
                     ERAGIRPKDVITKVNGKSIKGKALDEVVKDVRGKENTEVTLTVQRGSEEKDVKIKREK
                     IHVKSVEYKKKGKVGVITINKFQNDTSGELKDAVLKAHKDGLKKIVLDLRNNPGGLLD
                     EAVKMANIFIDKGKTVVKLEKGKDTEAIQTSNDSLKEAKDMDISILVNEGSASASEVF
                     TGALKDYNKAKVYGSKTFGKGVVQTTREFKDGSLLKYTEMKWLTPDGHYIHGKGIKPD
                     VTIDTPKYQSLNVIPNTKTFKVGDDDKNIKTIKIGLSALGYKVDNESTQFDQALENQV
                     KAFQQANKLEVTGEFNKETNNKFTELLVEKANKHDDVLDK"
BASE COUNT         2301 a          865 c         1101 g         1851 t
ORIGIN      
        1 attaccaatt ggaaacatat atcacgttac gatgtggtag gtacttaatc agcattttat
       61 aaataaagta acggaattca tgatattaat atcatattcc taaaatgagt gataacaaaa
      121 tgctacataa aattaagtta tatcaaacta aatatacata ctataaataa tgaaaatgag
      181 gtgttatcgc atatgttgaa ttcatttgat gcagcatatc acagtctttg tgaagaggtt
      241 ttagaaatag gaaatacacg aaatgatcgc acaaatacag gtacgatttc gaaatttggt
      301 catcaacttc gctttgactt atctaaagga tttccactat taacgacaaa gaaagtttct
      361 tttaaattag tagcaaccga attattatgg ttcattaaag gagatacaaa catccaatac
      421 ttattaaaat ataataataa tatttggaac gaatgggctt ttgaaaatta tataaaatca
      481 gacgagtata acggtccaga tatgacagat ttcgggcatc gtgcattgag tgatcctgaa
      541 tttaacgaac aatataaaga acaaatgaaa caatttaagc aacgtattct tgaagatgat
      601 acttttgcga agcaattcgg tgatttagga aatgtttatg gtaaacaatg gcgagattgg
      661 gttgataaag atggtaatca ttttgatcaa cttaaaacag taattgaaca aattaagcat
      721 aatccagatt caagacgaca catcgtatct gcatggaatc caacagaaat tgatacaatg
      781 gcacttccgc cttgtcatac catgttccag ttttatgtcc aagatggtaa gttaagttgc
      841 cagttatacc aacgtagcgc agatatcttt ttaggtgtgc catttaatat cgcaagctac
      901 gctttattga cacaccttat tgccaaagaa tgtggacttg aagtaggaga atttgtgcat
      961 acatttggag atgcacatat ttattcaaat catattgatg cgattcaaac acaattagca
     1021 cgtgaaagct tcaatcctcc aacattaaaa attaacagtg acaagtctat tttcgacata
     1081 aattatgaag atttggaaat tgttgactat gaatcacatc cagcaataaa agctccaata
     1141 gcagtgtagt cattgcatag ttagctaacc atatagacat caaaatgaca tcatagtatt
     1201 ttcaagtgca aaaaagtact tttttgtgtt aaacgttttc ataaattatg caaaatcatt
     1261 atttctatca cactttatga taaaaattgt gttaaattaa agataactta gtaataaaaa
     1321 atgaaatgat agaagaagga ggataattat gactttatcc attctagttg cacatgactt
     1381 gcaacgagta attggttttg aaaatcaatt accttggcac ctaccaaatg atttgaaaca
     1441 tgttaaaaaa ttatcaacag gtcatacttt agtaatgggt cgtaagacat ttgaatcgat
     1501 tggtaaacca ctaccgaatc gtcgaaatgt tgtacttact tcagatacaa gtttcaacgt
     1561 agagggcgtt gatgtaattc actctattga agatatttac caactaccgg gccatgtttt
     1621 catatttgga gggcaaacat tatttgaaga aatgattgat aaagtggacg acatgtatat
     1681 tactgttatt gaaggtaaat tccgtggtga tacgttcttt ccaccttata catttgaaga
     1741 ctgggaagtt gcctcttcag ttgaaggtaa actagatgag aaaaatacaa ttccacatac
     1801 ctttctacat ttaattcgta aaaaataagg gggaaaacga ccatgacaaa acagattata
     1861 gtaacagact caacatccga tttatctaaa gaatacttag aagcaaacaa cattcatgta
     1921 attcctttaa gtttaactat tgaaggagct tcatacgttg accaagtaga tattacatca
     1981 gaagaattta ttaatcatat tgaaaatgat gaagatgtaa agacaagtca gccagccata
     2041 ggtgaattta tatctgctta tgaagaacta ggaaaagatg gctctgaaat cataagtatt
     2101 catctttctt caggattaag tggtacatat aacactgctt accaagcaag tcaaatggta
     2161 gatgctaatg taactgttat tgattcaaaa tctatttctt ttggtttagg gtatcaaata
     2221 caacacctag tagagcttgt aaaagaaggt gtctcaactt ctgaaatagt taaaaagtta
     2281 aatcatttaa gagaaaacat taaattattt gtagttatag ggcaattgaa tcaattaatt
     2341 aaaggtggca gaattagtaa aacaaaaggt ttgattggta atcttatgaa aattaaacca
     2401 attggtacac ttgatgatgg tcgcttagag cttgtgcaca atgcgagaac tcaaaattct
     2461 agtatccaat acttgaaaaa ggaaattgct gaatttatag gagatcatga aatcaaatcc
     2521 attggtgtcg cacatgctaa cgtcattgaa tatgttgata aattgaagaa agtttttaac
     2581 gaagcttttc atgtgaataa ttacgatata aatgtaacta caccagttat ttctgcacat
     2641 actggtcaag gtgcgattgg cctcgtagtc cttaagaagt aaatttaatc ttttcagtgt
     2701 taattacttc catttcaatc ctttatagac taaatttata attagataga tagaggaggt
     2761 aattcatatg acaaaagaat atgcaacatt agcaggagga tgtttctggt gcatggttaa
     2821 accatttaca tcatatccag gcatcaagtc agtcgtatct ggttatagtg gcggtcatgt
     2881 tgataaccct acttatgaac aggtatgtac gaataaaacc ggccatgtcg aagcagtaca
     2941 aattacgttt gatccagagg ttacttcctt tgaaaatata ttagacatat atttcaaaac
     3001 atttgaccca actgatgatc aagggcaatt tttcgataga ggcgaaagct atcaaccagt
     3061 cattttctat catgatgagc atcagaaaaa ggctgctgag tttaaaaagc aacaattaaa
     3121 tgaacaaggt attttcaaga aaccagtgat tacacctatt aaaccatata aaaatttcta
     3181 tccagctgaa gactaccatc aagattatta taaaaagaac ccggtacatt attaccaata
     3241 tcaacgtggt tcaggtagaa aagcgtttat agaatcacat tgggggaatc aaaatgctta
     3301 aaaaagataa aagtgaacta acagacatag aatatattgt tacacaagaa aatggcactg
     3361 aaccaccatt tatgaatgaa tattggaatc attttgctaa agggatttat gtagataaga
     3421 tttctggtaa acctttattt acatctgaag aaaagtttca ttctgaatgt ggatggccta
     3481 gcttttctaa agcgcttgat gacgatgaaa ttatagaatt agtcgacaaa tcatttggta
     3541 tggtaagaac tgaagtgcgt tcagaagaat caaatagtca tttaggacac gtctttaatg
     3601 atggacctaa agaaagtggc ggcttaagat actgtatcaa ttccgctgca attcaattta
     3661 ttccatatga aaaattagaa gaattgggtt atggcgattt aatatcacat tttgataagt
     3721 aggagtggaa agtatgttta aaaaattatt cggaaaaggc aaagaagttc agaaagatat
     3781 cgcaatttat gcaccattaa ctggagaata cgtgaaaatt gaagatattc cagatcctgt
     3841 attcgcacaa aaaatgatgg gcgaaggttt tggtattaat ccaactgaag gagaagttgt
     3901 gtctccaatt gcaggacgtg ttgacaatgt ctttccaact aagcatgcga ttgggttaaa
     3961 agcagataac ggattagaat tattagttca tatcggttta gacacagttc aattagacgg
     4021 tgaaggattt gaagtgttag tgtctagtgg tgacgaagtt aacgtcggag atccattagt
     4081 aagattcaac cttgaattta ttaataataa cgctaaatct gttatttcac caattataat
     4141 tactaacact gatcaagcag cttcaattaa tatttatgat gaaaatgctg tgattaaagg
     4201 tgaaacaaaa gtgattgatg tgacaatgaa ctaatgaaaa actattcgtt ttatcaattt
     4261 gtcatgacag ttcgtggtcg acacgacgat aaaggtcgtc tagcagaaga gatatttgac
     4321 gatcttgctt tcccaaaaca cgatgatgat tttaacatac tgtctgatta tattgagaca
     4381 catggtgatt tcacattgcc aatgtctgta tttgatgatt tatatgaaga atatacggaa
     4441 tggctaaaat tttaatataa tttttaaaaa taatagttag aaccagggtg atgcaattcg
     4501 ttatcctggt tttaatttaa aataaactaa gttgtgacta aaaattaatc aattatagtg
     4561 aaatatggtg cgctatcttg cataaattga tatgattaac tacacagaat ttaaaagtac
     4621 ataatacata ataaggaagt gatacaatgg atgataagca acacacatct tcatccgatg
     4681 atgaacgcgc tgaaattgca acaagcaatc aagaccaaga aactaattca tcgaaacgcg
     4741 ttcacttaaa acgttggcaa ttcatatcaa tattaattgg cacaacccta ataacagctg
     4801 tcatcacagt tgttgcatat atttttataa atcaaaaaat aagtggttta aacaaaactg
     4861 atcaagcaaa cttaaataaa attgaaaatg tgtataaaat cttaaatagt gattattaca
     4921 aaaaacagga ctctgacaag ttaagtaaag ctgcaattga tggtatggtc aaagaattaa
     4981 aagatcctta ttctgaatat ttaacaaaag aacaaacgaa atcctttaat gaaggtgttt
     5041 caggtgattt tgtaggtatt ggtgcagaaa tgcaaaagaa aaatgatcaa attatggtta
     5101 ctagccctat gaagggttct ccagcagaac gtgctggcat ccgtcctaaa gatgtcatta
     5161 ctaaagtaaa tggaaaatca attaaaggaa aagcattaga cgaagttgtc aaagacgttc
     5221 gtggtaaaga aaacactgaa gtaactttaa ctgttcaacg aggcagtgaa gaaaaagacg
     5281 ttaagattaa acgtgaaaaa attcatgtta aaagtgttga gtataagaaa aaaggtaagg
     5341 ttggagttat tactattaat aaattccaga atgatacatc aggtgaattg aaagatgcag
     5401 ttcttaaagc tcacaaagat ggtttgaaaa agattgtttt agatttaaga aataatcctg
     5461 gtggactact agatgaagct gttaaaatgg caaatatttt tatcgataaa ggaaaaactg
     5521 ttgttaaact agaaaaaggt aaagatactg aagcaattca aacttctaat gactcgttaa
     5581 aagaagcgaa agacatggat atatccatct tagtgaatga aggttcagct agtgcttctg
     5641 aagtgtttac tggtgcgcta aaggactata ataaagctaa agtttatggg tcaaaaacat
     5701 tcggcaaagg tgtcgtacaa actacaagag agtttaagga tggttcattg ttaaaatata
     5761 ctgaaatgaa atggttaacg ccagatggtc attatattca cggtaagggc attaaaccag
     5821 acgttactat tgacacacct aaatatcaat ctttaaatgt cattcctaat acgaaaacat
     5881 ttaaagttgg agacgatgat aaaaatatta aaactattaa aattggttta tcagctttag
     5941 gttataaagt tgataatgaa tcaacgcaat ttgatcaagc tttagaaaat caagttaaag
     6001 ctttccaaca agcgaataaa ctcgaggtaa ctggtgaatt taataaagaa acgaataata
     6061 aatttactga gttattagtt gaaaaagcta ataaacatga tgatgttctc gataagtt
//
LOCUS       DAHHSC010000141         6114 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.142, whole genome shotgun sequence.
ACCESSION   DAHHSC010000141 DAHHSC010000000
VERSION     DAHHSC010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 6114)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6114)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6114
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.142"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            complement(81..929)
                     /locus_tag="NBI57_001978"
     CDS             complement(81..929)
                     /locus_tag="NBI57_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HCD1822911.1"
                     /translation="MNHIEISKDVKIPVLGFGVFQIPQEQTAEAVKEAIKAGYRHIDT
                     AQSYLNETEVGQGIEASGIDRSELFITTKVWIENVNYEDTIKSIERSLQRLNLDYLDL
                     VLIHQPYNDVYGSWRALEELKENGKIKAIGVSNFGVDRIVDLGIHNQIQPQVNQIEIN
                     PFHQQEEQVAALQQENVVVEAWAPFAEGKNQLFQNQLLQAIADKYNKSIAQVILRWLV
                     ERDIVVLAKSVNPERMAQNLDIFDFELTEEDKQQIATLEESNSQFFSHADPEMIKALT
                     SRELDV"
     gene            complement(999..1415)
                     /locus_tag="NBI57_001979"
     CDS             complement(999..1415)
                     /locus_tag="NBI57_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HCD1822912.1"
                     /translation="MKTKEVVALMNISQDTLRYYEKVGVIPPVNRDENGYRIYNDSDL
                     NWIYLVKNLRNAGVSIESLIEFCRLAQLPKNKNIQTQQKQILNKQLEELNENLKTIHD
                     ARDLLKYKIDNYDNHIAKINASDNHDDNVERLWERK"
     gene            1659..1826
                     /locus_tag="NBI57_001980"
     CDS             1659..1826
                     /locus_tag="NBI57_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1822913.1"
                     /translation="MRKIWVIDRLYASTFFVDCLTVDSHLYTLTNFENLNHYLIILLI
                     AHAQHKKETCQ"
     gene            1823..4252
                     /locus_tag="NBI57_001981"
     CDS             1823..4252
                     /locus_tag="NBI57_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide lyase 8 family protein"
                     /protein_id="HCD1822914.1"
                     /translation="MTYRMKKWQKLSTITLLMAGVITLNGGEFRSIDKHQIAVADTNV
                     QTPDYEKLRNTWLDVNYGYDKYDEKNDAMKKKFDATEKEAEKLLSSMKTESGRTYLWD
                     SAKDLDNKSADMTRTYRNIEKIAEAMRHPKTTLNTDENKKKVKDALEWLHKNAYGKEP
                     DNKVKELTENFKITDSSKKKALNWWDYEIGTPRSLTNTLILLNDQFSNEEKKKFTAPI
                     KTFAPKSDEILSSVGKAEPAKGGNLVDIAKVKLLESIIEEDKDMMKNSIDSFNKVFTY
                     VQDSATDKERNGFYKDGSYIDHKDVPYTGAYGVVLLEGISQMMPMIKETPFNDKTQNN
                     TTLKSWIDDGFLPLIYKGEMMDLSRGRAISRENETSHSASATVMKSLLRLSDAMDDST
                     KAKYKKIVKTSVKSDSSYGQNDTLSSYSDISKMKSLMEDSTISTNGLTQQLKIYNDMD
                     RVTYHNKDLDFAFGLSMTSKNVARYESINGENLKGWHTGAGMSYLYNSDVKHYRDNFW
                     ATADMKRLAGTTTLENEEPKGTDVKKSSKTFVGGTKFDDQHASIGMDFENQDKTLTAK
                     KSYFILNDKIVFLGTGIKSTDSSKNPVTTIENRKANGYTLYTDDKQTTASDNQGTNSV
                     FLESTNKPKNNIGYHFLNESKITVKKESHTGKWSDINKSQKQDSKTNQYYEVTQKHSN
                     TDSKYAYVLYPGLSKDDFNTKKDKVTVVKQDDDFHVVKDNESVWAGVNYSNSTQTFDI
                     NNTKVEVKAKGMFILKNKDDNTYECSFYNPESTNTASDIESKISITGYSITNKNTSTS
                     NESGVHFELTK"
     gene            complement(4312..5166)
                     /locus_tag="NBI57_001982"
     CDS             complement(4312..5166)
                     /locus_tag="NBI57_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HCD1822915.1"
                     /translation="MNKTNFGDYLNKSKSRKIYKIFDQNLKREFSYWQFRKLFKSYTR
                     QSDVHELKVCTQNDDTMEQIWIDKANQFGVAVTWKDNSIIGFVMRPLINYDAIKNETT
                     LKYAVPTTDCYEVFWGGDNELYNYHYLYLHQRYAIDLVIKENHKTYKNQGKVNTDYFC
                     FGKDIIAPANGTVEKVVNGVQDNSIGSTNESQFLGNYIVIKHAENEYSLIAHLQQYSI
                     IVNEGQNVKYGDFLGKVGNSGNSTEPHIHFQVMNDKNIEACTSLKIRFLNNLELIKGD
                     VVCGLQGE"
     gene            complement(5377..>6114)
                     /locus_tag="NBI57_001983"
     CDS             complement(5377..>6114)
                     /locus_tag="NBI57_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIB family hydrolase"
                     /protein_id="HCD1822916.1"
                     /translation="LREMEKLLEKLTNNNEVITAVLTGSTFQSVMDKMDQVNMTFKPL
                     HIFSDLSSKMFTWNNGEYVESETYKKKVLSEPFLFEDIEDILRHISAQYNVEFIPQRA
                     FEGNETHYNFYFHSTGNHNNDSRILEALVRYANDQNYTARFSRSNPLAGDPENAYDID
                     FTPSNAGKLYATQFLMKKYNIPVKSILGFGDSGNDEAYLSYLEHAYLMSNSRDEALKQ
                     KFRLTKYPYYQGITLHVKEFVEGKYDY"
BASE COUNT         2087 a         1025 c          908 g         2094 t
ORIGIN      
        1 taaaataacc tgcaacatag actttaacat ccaagtttca ggttagcgta atgtaaatta
       61 tttactttgt tatattatga ttaaacatct aattctctgc ttgtaagtgc tttaatcatc
      121 tctggatcag catgtgagaa aaattggcta ttactttctt ctaatgtcgc aatttgttgt
      181 ttatcttctt ctgtaagttc aaaatcaaat atatctaagt tttgagccat acgttctgga
      241 ttcactgatt ttgcgagtac aacaatgtca cgttctacta accagcgtaa aataacttgt
      301 gcaattgatt tgttgtattt atctgcaatg gcctgcaaca attgattttg gaataattga
      361 tttttacctt ctgcaaatgg tgcccaagct tctaccacaa cattttcttg ttgaagtgct
      421 gcaacttgtt cttcttgttg atggaatgga ttaatttcta tttggttcac ttgtggttga
      481 atttgattgt gaatacctaa atctacaatt cgatcaacgc cgaagttaga tacaccgatt
      541 gctttaattt taccattttc ttttaactct tctaatgcac gccatgagcc atatacgtca
      601 ttgtaaggtt ggtgaataag cactagatca agatagtcca aatttaatcg ttgtaatgat
      661 cgttcgattg atttgatagt atcttcatag ttaacatttt caatccatac tttagtcgta
      721 atgaatagtt cacttctatc aatacctgaa gcttcaatac cttgacctac ttcagtttca
      781 tttaaataac tttgtgctgt atcaatatgt ctataacccg ctttaattgc ttctttaacc
      841 gcttctgctg tttgttcttg aggaatttga aatactccga aacctaatac tggtatcttt
      901 acatctttac ttatttcaat atgattcata aaatgaacct cctttatctt gtatgtccac
      961 tataaaccat ggagcgaact ctatagcaag ccagaggttt actttctctc ccaaagacgt
     1021 tcaacattgt catcatgatt atcactagca ttgattttag caatatgatt atcataatta
     1081 tcaattttat attttagtaa atctctcgca tcatgaattg tctttaagtt ttcatttaat
     1141 tcttcgagtt gcttatttaa aatttgcttt tgctgtgttt gaatattttt atttttagga
     1201 agctgcgcta atctacaaaa ttcgataagc gattcaatac tgacgcctgc atttcgcaaa
     1261 tttttcacta aataaatcca atttaaatca ctatcattat atattctata tccattttca
     1321 tctcgattaa cgggtggaat cacaccaacc ttttcataat atcttaaagt gtcttgagat
     1381 atattcatga gcgctacgac ttctttagtt ttcattgcga ctatcctttc agttatattt
     1441 gggcatctaa agtaatgttg ttttatatat tgccatcttt gtttgaatac ttcttatttt
     1501 attactcaaa tttaaatttg tctctttttt aacattttac atttcatcgt ttttaattac
     1561 tttaaaaatt gtataactta aatatttaaa atgatataat cactaagatt gataatattt
     1621 aattttttaa aaattatttt tgttgcaatt tttgggtgat acggaaaatt tgggtgatcg
     1681 atcgtttata tgcatcaaca ttctttgtgg attgtttgac agtagacagt catttgtata
     1741 cgttaacgaa ttttgaaaat ttgaatcatt atttaatcat attattaatt gcgcacgcac
     1801 aacataaaaa ggagacatgt caatgacata tagaatgaag aaatggcaaa aattatccac
     1861 cattacgtta ttaatggctg gtgtgattac tttgaatggt ggtgaattca gaagtattga
     1921 taaacatcaa atcgctgtgg ctgatacgaa tgttcaaacg ccagattatg aaaagttgag
     1981 gaacacatgg ttagatgtta actacggtta tgataagtat gatgagaaga atgacgcaat
     2041 gaagaagaag tttgatgcta cggagaaaga ggcagagaaa ttactcagta gcatgaaaac
     2101 tgaaagtgga aggacttact tgtgggatag tgcaaaagat ttagataaca agtctgcgga
     2161 tatgactcgt acctatcgta atattgagaa aatcgcagaa gcgatgaggc atcctaaaac
     2221 cactttaaat actgacgaaa ataagaagaa agtgaaagat gcccttgagt ggctgcataa
     2281 aaatgcatat ggaaaagaac cagataataa agtaaaagaa ttgactgaga attttaaaat
     2341 aactgattca tcaaagaaaa aagctttaaa ttggtgggat tatgaaattg gaacacctag
     2401 atcattaaca aatacgctta tattgctgaa tgatcaattt tcaaatgaag aaaagaaaaa
     2461 attcactgcc cctattaaaa ctttcgcccc aaaaagtgat gaaatattat cttctgtagg
     2521 aaaagctgaa cctgctaaag gcggaaattt agtagacatt gctaaagtaa aacttttaga
     2581 aagtattatc gaagaagata aagatatgat gaaaaattca atagattcat ttaataaagt
     2641 cttcacttac gttcaagatt ctgccactga taaagaacgt aatggatttt ataaagatgg
     2701 ttcatatatc gaccataaag atgtcccata cactggtgct tatggcgttg tactcttaga
     2761 ggggatttct caaatgatgc cgatgataaa agaaacacct tttaatgata aaacccaaaa
     2821 taatacaacc ttaaaatcat ggattgacga cggattttta ccactcattt ataaaggtga
     2881 aatgatggat ttatcacgtg gtagagccat tagccgtgaa aatgaaacga gtcactcagc
     2941 atctgcaact gtaatgaaat cattgttgag attaagtgat gccatggatg attcaacaaa
     3001 agctaagtat aaaaaaattg tcaaaacttc agttaaatct gattcaagtt acggccaaaa
     3061 tgatacatta agctcttatt cggatataag caaaatgaag tctttaatgg aagacagcac
     3121 tatttctact aacggtttaa cacaacaact taaaatatat aatgacatgg atcgtgtcac
     3181 ctatcacaat aaagacttag actttgcatt tggtttaagt atgacgtcga aaaacgtcgc
     3241 acgttacgaa agtatcaacg gagagaactt aaaaggttgg cacactggtg ctggaatgtc
     3301 ttatttatac aatagcgatg tgaaacacta ccgtgataac ttctgggcga cagctgatat
     3361 gaaacgttta gcaggtacaa caactttaga aaatgaagaa cctaaaggta cggatgttaa
     3421 aaagtcgagt aaaacttttg taggcggaac aaaattcgat gaccaacatg ctagtatcgg
     3481 aatggatttt gaaaatcagg acaaaacttt aactgccaaa aaatcatatt tcatattaaa
     3541 cgataaaatt gtcttcttag gaactggcat taaaagtact gattcatcaa agaatccagt
     3601 tacaacgatt gaaaatcgca aagcgaatgg gtatacgtta tatacagacg ataaacaaac
     3661 aaccgcttcc gataatcagg gaaccaattc cgtcttttta gagtcaacca ataaacctaa
     3721 aaacaatata ggctatcatt ttttaaacga atcgaaaata actgtaaaaa aagaaagtca
     3781 tactggtaag tggagtgata taaataaaag tcaaaagcaa gacagtaaaa ctaatcaata
     3841 ttatgaagta actcaaaagc attctaatac agatagtaaa tatgcatatg tgttgtatcc
     3901 aggcttatct aaagatgact ttaatacaaa aaaagataaa gtaactgtcg ttaaacaaga
     3961 tgatgacttc cacgttgtga aagataatga atcggtttgg gctggtgtta attatagtaa
     4021 tagcactcaa acttttgaca ttaacaacac taaagtcgaa gttaaagcca aaggaatgtt
     4081 tattcttaaa aacaaagatg ataacactta tgaatgtagc ttctataatc ccgaatctac
     4141 aaataccgct tcagatattg aatctaaaat ttcaataact ggatactcta ttacaaacaa
     4201 aaatacatca acttctaatg aatccggcgt acactttgaa ttaactaaat aagcaagaaa
     4261 atctcgacac tataccattt ttatagtgtc gagattttat aagtatcgcc atcattcacc
     4321 ttgtaatccg cagaccacat cccctttgat aagttctaga ttatttaaaa atcgaatttt
     4381 caaagacgta catgcttcaa tattcttatc attcattact tggaaatgta tatgaggttc
     4441 agtagaattg ccagaattcc caaccttccc aaggaagtca ccatatttaa cattttgccc
     4501 ctcattcaca atgattgaat attgttgtaa atgagctatt aagctatact cattttctgc
     4561 gtgcttaatt acaatataat ttcctaaaaa ctgtgattca ttcgtacttc caatactgtt
     4621 gtcttgaacc ccattcacta ctttttcaac tgtaccattt gctggcgcaa tgatatcttt
     4681 tccaaaacaa aaataatcag tatttacttt tccttgattc ttatatgttt tatgattttc
     4741 tttaataact aaatcaatag catacctttg atgtaggtat aaatagtgat aattatataa
     4801 ttcattatct ccaccccaaa aaacctcata gcaatctgtt gtcggcactg cgtattttaa
     4861 agtcgtttca ttcttaatcg catcataatt gattaacgga cgcataacaa atccgataat
     4921 actattatct ttccaagtca ctgccactcc aaattgattt gctttatcta tccatatttg
     4981 ctccatagta tcatcatttt gagtacacac ttttaactca tgtacatcag actgccttgt
     5041 ataagatttg aaaagtttcc taaactgcca ataactaaac tctcgcttca aattttgatc
     5101 aaaaatctta tatattttcc tgctcttact tttatttaaa taatccccaa agtttgtttt
     5161 attcatatat acttaacctc ttatattaat tttggtatca attctttctc cagttcctgc
     5221 gtcatttttg cgctacctga atcgtctaca cttaattgga caaatcaaca tataaataat
     5281 tttttaaaac taaagtgtag tctattcacc atattttcta atcatccctt aaatattaat
     5341 atttgcactt aaaaagtata catatcactc aactaatcaa taatcatact taccttcaac
     5401 aaattcttta acatgcagtg ttatcccttg ataatatgga tattttgtta atctaaattt
     5461 ctgctttaat gcttcatctc tactattcga catcaaatat gcatgttcaa gatagcttaa
     5521 atatgcttcg tcattgccgc tatctccaaa acctaatatt gattttactg gtatattgta
     5581 ttttttcatc agaaattgag tagcatataa ttttccagca tttgacggtg tgaagtcaat
     5641 atcatacgca ttttcaggat caccagctaa tggattgctt cgactaaatc tcgctgtata
     5701 gttttggtca tttgcgtatc tgacaagtgc ttctaggata cgactatcat tgttgtgatt
     5761 acctgttgaa tgaaaataaa aattataatg tgtttcatta ccttcaaatg ctctttgtgg
     5821 aataaattcg acattatatt gcgcagaaat atgacgtaat atatcttcaa tatcttcaaa
     5881 taaaaatggt tcgctcaaga ctttcttttt atatgtttct gattcaacat attcgccatt
     5941 attccaagtg aacattttag aactcaaatc agaaaagata tgtaatggtt taaacgtcat
     6001 attaacttga tccattttat ccatgacact ttgaaatgta cttcctgtca atacagcagt
     6061 gatcacttca ttattattag ttagtttttc caacaatttt tccatttctc ttaa
//
LOCUS       DAHHSC010000142         6104 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.143, whole genome shotgun sequence.
ACCESSION   DAHHSC010000142 DAHHSC010000000
VERSION     DAHHSC010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 6104)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6104)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6104
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.143"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            complement(<1..1124)
                     /locus_tag="NBI57_001984"
     CDS             complement(<1..1124)
                     /locus_tag="NBI57_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family nicotinamide mononucleotide
                     deamidase-related protein"
                     /protein_id="HCD1822917.1"
                     /translation="MSIAIIAVGSELLLGQIANTNGQFLSKVFNEIGQNVLEHKVIGD
                     NKKRLESSVRHALEKYDTVILTGGLGPTKDDLTKHTVAQIVGKDLVIDEPSLKYIESY
                     FEEQGQEMTPNNKQQALVIEGSTVLANHHGMAPGMMVNFENKQIILLPGPPKEMQPMV
                     KNELLSHFINHNRIIHSELLRFAGIGESKVETILIDLIDKQTNPTIAPLAGSHEVYIR
                     LTANADSKEQAQSLIQPVKQEILDRIGEYYYGSDDTLIEQAVIKKIHEPFVIYDGITN
                     GALYHRLKEVDLNDVLKGMINHNENFVDINKPIEQQLKDAVQFVNKLFNVSSAIILLE
                     YDGVVHIGYDNNFEFKTEQFKMSKSRNLLKNRSQNYVLIR"
     gene            complement(1348..1926)
                     /gene="pgsA"
                     /locus_tag="NBI57_001985"
     CDS             complement(1348..1926)
                     /gene="pgsA"
                     /locus_tag="NBI57_001985"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HCD1822918.1"
                     /translation="MNIPNQITVFRVVLIPVFILFALVDFGFGNVSFLGGYEIRIELL
                     ISGFIFILASLSDFVDGYLARKWNLVTNMGKFLDPLADKLLVASALIVLVQLELTNSV
                     VAIIIIAREFAVTGLRLLQIEQGFVSAAGQLGKIKTAVTMVAITWLLLGDPLATLIGL
                     SLGQILLYIGVIFTILSGIEYFYKGRDVFKQK"
     gene            complement(1960..2352)
                     /locus_tag="NBI57_001986"
     CDS             complement(1960..2352)
                     /locus_tag="NBI57_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCD1822919.1"
                     /translation="MKTVGEALKGRRERLGMTLTELEQRTGIKREMLVHIENNEFDQL
                     PNKNYSEGFIRKYASVVNIEPNQLIQAHQDEIPSNQAEWDEVITVFNNNKDLDYKSKS
                     KEPIQLLVIMGITVLITLLLWIMLVLIF"
     gene            complement(2371..3198)
                     /locus_tag="NBI57_001987"
     CDS             complement(2371..3198)
                     /locus_tag="NBI57_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmfK family protein"
                     /protein_id="HCD1822920.1"
                     /translation="MTVAEKKEWYLEYEIEINRPGLLGDVSSLLGMLGISIVTINGVD
                     QGKRGLLIKTDNLEKVERFEQIARGINEIEITKLKKPELRDRLAVRHGRYIEQDAKDK
                     KTFRFEREDLGLLVDFLAELFKEEGHKLIGIRGMPRVGKTESIVAGSVCAHKRWLFIS
                     STLIKQTVRSSLIKGEYDANHVYIIDGAVTARESNPKHQELVNEVMTLPSIKVVEHPD
                     LFVETSTCTMEDFDYIIELRENENQEIHYEEMKKQTVQSKNNLDFGDPFGGGFGFFE"
     gene            complement(3303..4007)
                     /locus_tag="NBI57_001988"
     CDS             complement(3303..4007)
                     /locus_tag="NBI57_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HCD1822921.1"
                     /translation="MKALVLGGSGSIGSEIVKQLLTDGFEVYVQYYRTDINELTSKFN
                     DDKVRFIQADLSQTIDIDKTFGDIKSLDCLIYASGQSLYGVLQDMKDHDIDACYQLNV
                     LQLIRLCRYFVDVLRQSANGRIIVISSIWGETGASMETIYSAMKSAQLGFVKALSQEL
                     ALTSVTVNAIAPGFVAGNMASEWQEDELQAMITELPQQRLVLPSEVAHTCAYLYHSNA
                     RSVTGTIQKVNGAWYI"
     gene            complement(4007..5293)
                     /locus_tag="NBI57_001989"
     CDS             complement(4007..5293)
                     /locus_tag="NBI57_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HCD1822922.1"
                     /translation="MKERYYELIDERVFEQELENGLRLFIIPKPGFQKTFVTYTTQFG
                     SLDNQFKPLGQDQFVTVPDGVAHFLEHKLFEKEEEDLFTAFAEDNAQANAFTSFDRTS
                     YLFSATDNIENNIKRLLTMVETPYFTKETVDKEKGIIAEEIKMYQEQPGYKLMFNTLR
                     AMYQRHPIRVDIAGSVESIYDITKDDLYLCYETFYHPSNMVLFVVGDVNPEEICRIVK
                     QHEDARNKVNQPKIERGLVDEPEDVKEAFVTESMKIQSPRLMLGFKNKPLQEAPQKYV
                     QRDLEMSLFFELIFGEETDFYQNLLNEGLIDDTFGYQFVLEPTYSFSIVTSATEEPDK
                     LKKLLLDELRDKKGNFQDAEAFELLKKQFIGEFISSLNSPEYIANQYTKLYFEGVSVF
                     DMLDIVENITLDSINETSSLYLNLDQQVDSRLEIKK"
     gene            complement(5293..>6104)
                     /locus_tag="NBI57_001990"
     CDS             complement(5293..>6104)
                     /locus_tag="NBI57_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HCD1822923.1"
                     /translation="FLKLLDHMFENEAYKYLSTGQLEQIPHITAETLYHTYQSMINND
                     QCSVYVVGNVEPESVEKQIREKFALKPFDKHQFQHSTHHLHDEEVDYIVEYDDVDQAK
                     LNMGYRFPTQYGQSGYAAFVVFNMMFGGDPSSVLFNEVREKQSLAYSIHSQIDGKNGY
                     LFVLSGVSSDKYETAKDTIISEFEKIKAGDFTEEKLELAKKVIISHRYESEDRPKSII
                     EIMHNQILLEQPQSKETFINDIQKVSREDIVSVAEKAFLDTIYVLTKGGDK"
BASE COUNT         1966 a         1143 c          765 g         2230 t
ORIGIN      
        1 aatcttatga gcacataatt ttgacttctg ttctttaata aatttctaga tttagacatt
       61 ttaaattgct cagttttaaa ttcaaagtta ttatcatagc ctatatggac tacaccatca
      121 tactctaata gaataattgc tgatgacaca ttaaacaatt tattaacaaa ttgcactgca
      181 tcttttaatt gctgctcaat aggtttatta atatcaacaa agttttcatt gtgattaatc
      241 atacccttta gaacatcgtt taaatccact tctttcaatc gatgatataa agcaccatta
      301 gtaataccat catatattac aaaaggttca tgaattttct ttattacagc ttgctcaatt
      361 aatgtgtcat ctgaaccata ataatattct ccaatacgat caagaatttc ttgtttaaca
      421 ggttgaatca atgattgtgc ttgttctttt gagtcggcat ttgcagtcaa tctaatatat
      481 acttcatgac ttcccgccaa aggcgcaatc gtaggattag tctgtttatc gataagatct
      541 attaatattg tttctacttt agattcacct attcccgcaa atcttaatag ttcagaatgt
      601 ataattcgat tatggtttat aaaatgtgac aacaattcat ttttcaccat tggttgcatt
      661 tctttcggtg gacctggtaa taaaataatt tgtttgtttt caaaattcac catcattcct
      721 ggagccatgc catgatgatt tgctaataca gttgaacctt caattactaa agcctgttgt
      781 ttattattag gtgtcatttc ttgtccttgt tcctcaaaat agctttcaat atattttaaa
      841 gaaggctcat caataactaa atctttacca acaatctggg ccactgtatg cttcgttaag
      901 tcatctttcg taggacctaa gccacctgtt aaaataacag tatcatattt ttctaacgca
      961 tgacgtacac ttgattctaa acgtttttta ttatctccaa taactttatg ttctaataca
     1021 ttttgtccaa tttcattaaa tactttagat agaaattgtc cgttggtatt agcgatttga
     1081 cctagcaata gttctgagcc tacagcaata atggcaattg acatttatat cccccttgta
     1141 tcgtttaatt cttttttcat gtattccatt taaaaattca catcaatttt agattcaatg
     1201 tatcagatgc tacaacacat ttgcctaaaa aatgaacaaa tattgtttta caatatatca
     1261 ttataaacaa ttgcgtggct aatttctaaa attgggatta aaaaaactag attccatatg
     1321 aaaatgaaat ctagtataaa caaatattta tttttgttta aaaacatctc tacctttata
     1381 aaagtattca ataccagata agatagtaaa aataacgcca atgtataata aaatttgtcc
     1441 taatgacaaa ccaatcaatg ttgccaatgg atcacctaat aacaaccaag taattgctac
     1501 catagtaact gctgttttaa ttttacctaa ttgaccagct gcacttacaa atccttgttc
     1561 aatttgtagt aaacgtaaac cagttacggc aaattctctg gcaataataa tgattgctac
     1621 tacagaattt gttagttcta gttgcacaag tacaattaaa gcacttgcaa ctaataattt
     1681 atccgctaat ggatccaaaa atttccccat atttgtaact aaattccatt ttctagctaa
     1741 ataaccatca acaaaatcgc taagggaagc caatataaaa ataaaaccac tgattaataa
     1801 ctcaattctt atttcatatc ctcctagaaa tgacacattg ccaaatccaa aatcaactaa
     1861 cgcaaacaat ataaaaactg gtattaacac tactctaaaa accgtaatct ggttcggaat
     1921 attcattata catcctcatt tctcactaat ttatttctgt taaaatatta aaactaacat
     1981 gatccataac aataaagtta ttaaaactgt aatacccatg attactaata attgtattgg
     2041 ctcttttgat ttactcttat aatctaagtc tttattatta ttgaaaactg taattacttc
     2101 gtcccattcg gcttggttcg atggaatttc atcttgatga gcttgaatta attggttagg
     2161 ttcaatattt actacgcttg catattttct aataaatcct tcgctgtaat ttttattcgg
     2221 tagttgatcg aattcattat tttcaatatg cactagcatt tcacgtttaa ttccagtacg
     2281 ttgctctaat tctgttaaag tcattcctaa cctttcacgt ctacctttta gcgcttcacc
     2341 gaccgttttc aattcatagc ctccttacac ttactcgaag aaaccaaaac caccaccaaa
     2401 cggatctcca aaatctaaat tattcttact ttggactgtc tgtttcttca tttcttcgta
     2461 atgtatttct tgattttcat tttctctcaa ttcgataata tagtcaaaat cttccattgt
     2521 acaagtactt gtttcaacaa ataaatctgg atgttcaacg actttgattg atggtaacgt
     2581 cataacttcg ttaacaagct cttgatgttt tggattagat tctctggcag taactgcacc
     2641 atcaataatg tatacatgat tggcatcata ttccccttta attagagagc tacgtacagt
     2701 ttgttttatt aaagtagaac taataaataa ccatctctta tgtgcacaaa cacttcccgc
     2761 aacaattgat tcagttttac caacccgtgg catacctcta atgccaatca acttatgacc
     2821 ttcttctttg aacaattcag ctaaaaagtc tactaacaag cctaaatctt cacgctcaaa
     2881 tcgaaaggtt ttcttatctt ttgcatcttg ctcaatatat ctaccatgtc taactgcaag
     2941 acggtctctt aattctggtt ttttaagctt tgttatttca atttcattta taccacgagc
     3001 tatttgctca aaacgttcaa ctttttcaag attgtctgtt ttaattaaaa ggcctcgttt
     3061 accttgatca acaccattaa ttgtaacaat acttatacct aacataccta ataaactaga
     3121 tacatcacct aaaagacccg gtctattaat ttcaatttcg tattctaggt accattcttt
     3181 tttctctgca actgtcatgg tgaacctcac cccttgttta attaacaaat tatgattcat
     3241 tgtaatattc atattataac atcaatcata aaaacatttg ctcataaaaa tagttctata
     3301 ttttaaatat accaagcacc attaactttc tgtatagttc cagtaacgct tctagcattt
     3361 gagtgatata aataggcgca tgtatgagca acctcactcg gtaaaaccaa tcgctgttgt
     3421 ggtaattcag ttatcattgc ttgaagttca tcttcttgcc actcacttgc catattaccg
     3481 gctacaaatc caggtgcgat agcatttact gtcactgatg ttagtgcaag ctcttgacta
     3541 agcgccttaa cgaaacctaa ttgtgcactt ttcatcgccg aataaatagt ttccatacta
     3601 gctcctgtct caccccaaat tgatgaaatt acaataattc ttccattggc actttgacgt
     3661 aaaacatcaa cgaaatatct acataatcga attaattgca agacatttaa ctgataacat
     3721 gcatcaatat catggtcttt catatcttgt aaaacaccat ataaagactg accacttgca
     3781 tatattaaac agtctaatga tttaatgtca ccaaatgttt tgtcaatatc aattgtttga
     3841 gataaatccg cttgtataaa acgaacttta tcatcattaa atttgctagt taattcattt
     3901 atatcagtac gataatattg cacataaact tcaaatccat cagttaataa ttgtttgact
     3961 atctcagaac caattgaacc agaaccacct aatactaatg ctttcattac tttttaatct
     4021 ccaaacgact atcgacttgc tgatctaaat ttaaatataa cgatgacgtt tcgttaatac
     4081 tatctaacgt gatattttca acaatgtcta acatgtcaaa cacgctaaca ccttcaaaat
     4141 acaatttagt atattgatta gcaatatatt caggtgagtt taaacttgat atgaattcac
     4201 ctataaattg ctttttcaaa agttcaaatg cttctgcatc ttggaaattg ccttttttat
     4261 cacgcaactc atctaataat aattttttta atttatctgg ttcttcagta gcgcttgtca
     4321 cgattgaaaa actatacgtc ggctctagta caaattgata accaaatgta tcatcgataa
     4381 gtccttcgtt taataaattc tgataaaaat ctgtttcttc cccaaaaatt aactcaaaga
     4441 ataatgacat ttctaaatca cgttgtacat atttttgagg cgcttcttgt aatggtttat
     4501 ttttaaaacc aagcattagt cttggtgatt gaattttcat agattcagta acaaatgctt
     4561 ctttaacatc ctccggttca tcaacaagtc ctcgttcgat tttgggttgg ttaactttat
     4621 tacgagcatc ctcgtgttgt tttactattc gacatatttc ttctggattc acatcaccaa
     4681 caacaaataa aaccatattt gatggatgat aaaacgtttc ataacataga tacaaatcat
     4741 cttttgtaat atcgtatata ctttctacac taccggcaat atcaacacgt attggatgtc
     4801 gttgatacat tgcacgcaat gtattaaaca ttaatttata tccaggttgt tcttgataca
     4861 tttttatttc ttctgcaata ataccttttt ctttatcaac agtttctttt gtaaaataag
     4921 gcgtttcaac cattgtaagt aaacgtttaa tgttgttttc aatattatca gttgcactga
     4981 acaagtagct tgtacgatca aagcttgtaa acgcatttgc ttgtgcatta tcttcagcaa
     5041 acgcagtaaa taagtcttct tcttcttttt caaataattt atgttctaaa aagtgagcaa
     5101 ctccatcagg tacagtaaca aattggtctt gtccaagggg tttgaattga ttatctaatg
     5161 aaccaaattg tgtagtgtaa gtgacaaatg tcttttgaaa acctggtttg gggataataa
     5221 ataatcgtaa accattttct aattcttgtt cgaatactct ttcgtctatt aattcataat
     5281 aacgctcttt cattatttat cccctccttt tgtcaacaca tagattgtat ctaaaaatgc
     5341 tttttcagca acagaaacaa tatcttcgcg acttaccttc tgtatatcat ttataaatgt
     5401 ttctttgctt tgaggttgct ctaataatat ttggttatgc ataatctcta taatactttt
     5461 cggacgatct tcagattcat atcgatgaga aatgattact tttttagcta actctaattt
     5521 ttcttcagtg aaatctcctg cttttatttt ttcaaattca cttataatag tgtcttttgc
     5581 agtttcgtac ttatcacttg aaaccccact caaaacaaat aaatagccat ttttgccatc
     5641 aatttgtgaa tgtatagagt acgctaaact ttgcttttct cgcacttcat taaataaaac
     5701 agatgaagga tctcctccaa acatcatgtt aaatacaaca aacgcagcat atccactttg
     5761 tccatattgt gttggaaaac ggtatcccat atttaattta gcttgatcca cgtcatcata
     5821 ttcaacaata taatcaactt cttcatcgtg taaatgatga gtagaatgtt ggaattgatg
     5881 tttatcgaat ggtttaagtg caaatttttc acgtatttgt ttctcaacac tttcaggttc
     5941 tacattgccg acaacataaa cagaacattg atcattatta atcattgatt gatatgtatg
     6001 atatagtgtt tcagcagtaa tatgtgggat ttgttctagt tgtcctgtag ataagtattt
     6061 atatgcttca ttttcaaaca tatggtcgag taattttaaa aacg
//
LOCUS       DAHHSC010000143         6096 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.144, whole genome shotgun sequence.
ACCESSION   DAHHSC010000143 DAHHSC010000000
VERSION     DAHHSC010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 6096)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6096)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6096
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.144"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            113..1051
                     /locus_tag="NBI57_001991"
     CDS             113..1051
                     /locus_tag="NBI57_001991"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HCD1822924.1"
                     /translation="MANIVFKLSDKDITTLMSRITFDTENLPQGMKARAKYQNTTVNI
                     YQSGKVMFQGNHAEAVSKELLPQHSQLNTNKTKKKNMANSSLEQTLMYDQFNCIGSDE
                     AGSGDYFGPLTVCAAFVTKEHVPILKTLGVDDSKKLTDTKIVELAEQLVAFIPHSLLT
                     LHNDKYNIQQAKGWTQVKMKAVLHNEAIKNVLEKIDSSQLDYIVIDQFAKREVYSHYA
                     LSDIPLPKKTKFETKGESKSLAIAVASIISRYAFITYMDQISKYINMTIPKGAGAKVD
                     VIAAKIIKKYGLSRLDTISKKHFKNREKAQKILKPL"
     gene            complement(1483..3885)
                     /locus_tag="NBI57_001992"
     CDS             complement(1483..3885)
                     /locus_tag="NBI57_001992"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HCD1822925.1"
                     /translation="MLISNEWLKEYVTIDDSVSDLAERITRTGIEVDDLIDYTKDIKN
                     LVVGFVKSKEKHPDADKLNVCQVDIGEDEPVQIVCGAPNVDAGQYVIVAKVGGRLPGG
                     IKIKRAKLRGERSEGMICSLQEIGISSNYIPKSFESGIYVFSESQVPGTDALQALYLD
                     DQVMEFDLTPNRADALSMIGTAYEVAALYNTKMTKPETTSNELELSANDELTVTIENE
                     DKVPYYSARVVHDVTIEPSPIWMQARLIKAGIRPINNVVDISNYVLLEYGQPLHMFDQ
                     DAIGSQQIVVRQANEGEKMTTLDDTERELLTSDIVITNGQTPIALAGVMGGDFSEVKE
                     QTSNIVIEGAIFDPVSIRHTSRRLNLRSESSSRFEKGIATEFVDEAVDRACYLLQTYA
                     NGKVLKDRVSSGELGAFITPIDITADKINRTIGFDLSQNDIVTIFNQLGFDTEINDDV
                     ITVLVPSRRKDITIKEDLIEEVARIYGYDDIPSTLPVFDKVTSGQLTDRQYKTRMVKE
                     VLEGAGLDQAITYSLVSKEDATAFSMQQRQTIDLLMPMSEAHASLRQSLLPHLIEAAS
                     YNVARKNKDVKLFEIGNVFFANGEGELPDQVEYLSGILTGDYVVNQWQGKKETVDFYL
                     AKGVVDRVSEKLNLEFSYRRADIDGLHPGRTAEILLENKVVGFIGELHPTLAADNDLK
                     RTYVFELNFDALMAVSVGYINYQPIPRFPGMSRDIALEVDQNIPAADLLSTIHAHGGN
                     ILKDTLVFDVYQGEHLEKGKKSIAIRLNYLDTEETLTDERVSKVQAEIEAALIEQGAV
                     IR"
     gene            complement(3885..4943)
                     /gene="pheS"
                     /locus_tag="NBI57_001993"
     CDS             complement(3885..4943)
                     /gene="pheS"
                     /locus_tag="NBI57_001993"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HCD1822926.1"
                     /translation="MSEQQTMSELKQQALVDINEANDERALQEVKVKYLGKKGSVSGL
                     MKLMKDLPNEEKPAFGQKVNELRQTIQNELDERQQMLVKEKLNKQLAEETIDVSLPGR
                     HIEIGSKHPLTRTIEEIEDLFLGLGYEIVNGYEVEQDHYNFEMLNLPKSHPARDMQDS
                     FYITDEILLRTHTSPVQARTMESRHGQGPVKIICPGKVYRRDSDDATHSHQFTQIEGL
                     VVDKNVKMSDLKGTLELLAKKLFGADREIRLRPSYFPFTEPSVEVDVSCFKCKGKGCN
                     VCKHTGWIEILGAGMVHPNVLEMAGFDSSEYSGFAFGMGPDRIAMLKYGIEDIRHFYT
                     NDVRFLDQFKAVEDRGDM"
     gene            complement(5323..6063)
                     /locus_tag="NBI57_001994"
     CDS             complement(5323..6063)
                     /locus_tag="NBI57_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HCD1822927.1"
                     /translation="MEQITSAQNNRIKQANKLKKKRERDKTGLALIEGVHLIEEAYQS
                     GIVITQLFAIEPARLDQQIIAYAQEVFEINMKVAESLSGTVTPQGFFAIIEKPHYDIS
                     KAQQVLLIDRVQDPGNLGTLIRTADAAGMDAVIMEKGTTDPYQDKVLRASQGSVFHLP
                     VMTQDLDTFITQFNGPVYGTALENAVAYKEVTSSDSFALLLGNEGEGVNPELLAHTTQ
                     NLIIPIYGKAESLNVAIAGSILLYHLKG"
BASE COUNT         1940 a         1184 c          885 g         2087 t
ORIGIN      
        1 gtatccgcaa ttaattcttt acaacataca taaatgtttc tgacgttatt atcatttatg
       61 atatgattat ttttgctaat gataatgaaa ttatatgaaa ggatttacac aaatggcgaa
      121 tatcgttttt aaattgtcgg ataaagacat aacgacatta atgtcacgca ttacttttga
      181 tactgagaat ttacctcaag gtatgaaagc acgtgcaaag tatcaaaata caactgtaaa
      241 tatttaccaa tcaggtaaag tcatgtttca aggtaatcat gcagaagctg tgtctaaaga
      301 acttctaccg cagcattctc aattgaatac taataaaact aaaaagaaaa atatggctaa
      361 ttcttctctt gaacaaactt taatgtatga tcaatttaac tgtattggta gtgatgaggc
      421 tggaagtggc gattactttg gtcctttgac tgtgtgtgct gcttttgtta ccaaagagca
      481 tgttcctatt ttaaaaactt tgggtgtaga tgattcaaaa aaattaaccg ataccaaaat
      541 tgtagaactt gctgagcaat tggttgcttt catcccacat tcactattaa cactacacaa
      601 tgataaatat aatattcagc aagcaaaagg ctggactcaa gtaaaaatga aagctgttct
      661 tcataatgaa gccatcaaaa atgtacttga aaaaattgat tcgtctcaat tagactatat
      721 cgtcattgat cagttcgcaa aacgcgaagt gtatagccat tacgcattat ccgatatacc
      781 acttccaaag aaaacaaaat ttgaaacaaa aggagaatcg aagtcattag ccattgcagt
      841 ggcaagcata atttctagat acgcatttat tacatatatg gatcaaatat caaaatatat
      901 taatatgacg attcctaaag gtgccggtgc taaagtcgat gtaattgccg caaaaattat
      961 aaaaaaatac ggtctttcac gtttagatac catttcaaaa aagcatttta aaaatcgtga
     1021 aaaagcccaa aaaattttaa agcctctttg atttttaatc attagaggct tttatgcagt
     1081 tgtgcgaagc atcatctgta ttccgctgtt cctctttgat ttataaaatg aattggatcc
     1141 taataggtga tcctattagg taagtcatac taatttgctt tgctcataag tatgacttta
     1201 tatgcagtga tgcgaagcat catctgtaat ccactgtgcc tctatgattt ataaaatcaa
     1261 gataaattgg atcctaatag gaaatcctat taggtaagtc atactaattt gctttgctca
     1321 taagtatgac tttatatgca gttgtgagaa gcatcatctg tattccgctg tgcctctttg
     1381 atttttaaat gaatcgaaac ctaattggtg aatccaaaaa taaaacccca tgttaaggat
     1441 atcaatctac ttcagatatc cttatacatg gggtttaaat cattatctaa taacagcacc
     1501 ttgttcaatt aatgctgctt caatttccgc ttgcactttt gaaacgcgct catctgtcaa
     1561 tgtttcttct gtgtctaaat aatttaaacg tattgcaatt gattttttac ctttttctaa
     1621 atgttcgccc tgatatacat caaagacaag tgtatctttt aatatattgc caccgtgtgc
     1681 atgaatcgtt gataataaat cagctgctgg aatattttga tctacttcta atgcaatgtc
     1741 acgagacatg cctgggaatc tcggaattgg ctggtaatta atgtaaccta ccgacacagc
     1801 cattaatgca tcaaaattca actcaaaaac atacgtacgt tttaaatcat tatcagctgc
     1861 taatgttgga tgtaattcac caataaaacc aacaacttta ttctctaata agatttcagc
     1921 agtacgacct ggatgtaatc catcaatatc agcacgacga taactaaatt caaggtttaa
     1981 cttttcagat actcgatcca cgacaccttt tgctaaatag aaatcaaccg tttctttctt
     2041 accttgccat tgattgacta cataatctcc agttaaaata ccacttaaat attcaacttg
     2101 atctggtagt tcaccttctc cattagcaaa gaagacattg ccgatttcaa ataattttac
     2161 atctttattt ttgcgtgcca cattatatga tgctgcttcg attaaatgtg gtaataaact
     2221 ttgacgtaat gacgcatgcg cttcactcat tggcatcaat aaatcaattg tttgacgctg
     2281 ttgcatcgaa aatgcagtag catcctcttt agaaactaac gaatacgtaa tagcttgatc
     2341 taatccagca ccttctaaca cttctttaac cattctagtt ttatattggc gatcagttag
     2401 ctgaccacta gtaactttat cgaagacagg taacgttgat ggaatatcgt cgtatccata
     2461 tatacgtgca acttcttcaa ttaaatcttc tttaattgta atatctttac gacgtgatgg
     2521 tactagcact gtaataacat catcatttat ttctgtatca aaccctagtt gattaaaaat
     2581 agtaacaata tcattttgtg acaaatcaaa tccaatagtg cgattaattt tatcagcagt
     2641 gatgtcgatt ggtgtaataa atgcaccaag ttctcctgaa gacactctat cttttagcac
     2701 ttttccgttt gcataagttt gtaataaata acatgcacgg tcgactgctt catctacaaa
     2761 ttcagtagct attccttttt caaaacgact agatgattca ctgcgtaaat ttaaacgtct
     2821 tgatgtatga cgaattgaaa ctggatcaaa aatagcacct tcaatcacta tatttgatgt
     2881 ttgttcttta acttctgaaa aatcgccacc cataacacca gctaatgcaa ttggagtttg
     2941 tccattagta atgacaatat cgctcgttaa taattcacgt tctgtatcat ctaatgttgt
     3001 cattttttcg ccttcattag cttgacgaac aacaatttgt tgtgaaccaa tcgcatcttg
     3061 atcaaacatg tgcaatggtt gaccgtattc taataacaca taatttgaaa tgtcaacaac
     3121 attattaata ggacgtatac ccgcttttat taagcgtgct tgcatccaaa ttggcgaagg
     3181 ttcaattgtc acgtcgtgaa caacacgtgc actataatat ggtactttat cttcattttc
     3241 aattgtcaca gtcaattcat catttgcaga taactcaagc tcatttgatg ttgtctctgg
     3301 cttagtcatt tttgtattat ataatgctgc aacttcataa gcagtaccta tcatacttaa
     3361 agcatctgca cgattcggcg ttaaatcaaa ttccattact tgatcatcta aatataaagc
     3421 ttgtaaggca tctgttcctg gaacttggga ttcactaaaa acataaatgc ctgattcaaa
     3481 acttttcggt atatagttac ttgaaatacc aatttcttgt aacgaacaaa tcataccttc
     3541 tgaacgttca ccgcgtaatt tggcacgctt aattttaata ccaccaggca atctgccacc
     3601 tactttagca acaatgacat attgtcctgc atcaacgttc ggtgcaccac atacgatttg
     3661 tacaggttcg tcttctccga tatcaacttg gcaaacattt aatttatcgg catcaggatg
     3721 tttctctttt gacttaacga aaccgacaac taaatttttg atatcttttg tgtagtcaat
     3781 taaatcatcc acttcaatac ctgtgcgcgt aatacgttct gccaaatcac ttacagaatc
     3841 atcgattgtt acatattctt tcaaccattc atttgatatc aacattacat gtcacctcta
     3901 tcttctaccg ctttaaattg atctaaaaat ctcacatcat tagtatagaa atgacgaata
     3961 tcttctatac catatttcaa cattgcaata cggtctggtc ccataccaaa tgcaaatcca
     4021 gagtactctg aagaatcaaa accagccatt tctaatacat taggatgtac cattccagca
     4081 cctaaaattt caatccatcc tgtgtgttta cacacattac aaccttttcc tttacattta
     4141 aaacatgaca catcaacttc tacagaaggt tcagtgaatg ggaagtaact tggacgtaaa
     4201 cgaatttcac gatcagcacc aaataatttc ttagctaaca attctaaagt gcctttcaaa
     4261 tcactcattt taacgttttt atcaacaact aatccttcga tttgtgtaaa ttgatgacta
     4321 tgtgtcgcat catcagagtc acgacgatac actttaccag ggcaaataat tttaactgga
     4381 ccttgaccat gacgtgattc catcgtacgt gcctgcactg gtgatgtatg cgtacgtaat
     4441 aaaatttcat ccgtaatata gaaactatct tgcatatcac gtgcagggtg tgatttaggt
     4501 aaattcagca tttcgaagtt ataatgatct tgttcaactt catatccatt cacaatttca
     4561 taacctaaac ctaagaataa gtcttcaatt tcttctattg tacgtgttaa tggatgcttt
     4621 gaaccgattt caatatgacg acctggtaat gatacatcaa ttgtttcttc agccaattgc
     4681 ttatttaatt tttctttaac taacatctgt tgtctttcat ctaattcatt ttgaattgtt
     4741 tgacgcaatt cattcacttt ttgaccaaac gcaggtttct cttcattcgg caaatccttc
     4801 atcaatttca ttagtccgct aactgaccct tttttaccta agtatttcac tttaacttct
     4861 tgcagtgcac gttcatcatt tgcttcatta atatctacaa gcgcttgttg ttttaactct
     4921 gacattgttt gttgttcaga catatgtcac tgacctccct attcgaacta ttcattttct
     4981 attgatcata tttgttgttt aactaaaaaa tgactttcat cctcttaaaa ataagggacg
     5041 aaagtcattt ccgtggtacc acccaaattt atgcctaagc atacacttgt ttaatattga
     5101 taacggattg tctttccgac ttaaatctct ttaagtaacc aaaaaggtga aaagacttta
     5161 aataattgga ataacttgca gtcacgatta ttctccctaa aacaattatt tatataaatg
     5221 tccatgcctt tttattggtt tatattcaat tgtgtatgca ttcacgtaga aatgttctgt
     5281 taaacaatta attataatta tatcggaaaa ctttcaacac ggtcaacctt tcaaatgata
     5341 aagtaaaata ctacctgcaa tcgctacatt taaactttca gctttaccat aaataggtat
     5401 gattaaattt tgtgtagtat gtgctaataa ttcaggatta acaccttctc cctcattacc
     5461 taatagtaat gcaaaagaat cacttgaagt aacttctttg tatgccactg cgttttcaag
     5521 tgctgtacca taaacaggac cattaaattg agtaataaac gtatcgagat cttgtgtcat
     5581 aactggcaaa tggaaaacac taccttgact cgctcgcaac actttatctt gataaggatc
     5641 tgtcgtaccc ttctccatta ttacagcatc cattccagca gcatccgcag ttctaattaa
     5701 tgtgcctaaa tttccaggat cttgaacacg atcgatgagc aatacttgtt gtgctttaga
     5761 aatatcataa tgcggcttct caatgattgc gaaaaaccct tgtggtgtca ctgtacctga
     5821 taaagattca gcaactttca tgtttatttc aaaaacttct tgcgcgtatg cgataatttg
     5881 ctgatctaat cttgccggtt caattgcaaa taattgtgta attacaattc cactttgata
     5941 agcttcttca attaaatgca caccttcaat taaagctaat ccagttttat ccctctcacg
     6001 tttctttttt agcttgttcg cttgtttaat tctattattt tgtgcagaag taatttgttc
     6061 cattgatagc tcctcgcttt atttttaaaa ataaaa
//
LOCUS       DAHHSC010000144         6075 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.145, whole genome shotgun sequence.
ACCESSION   DAHHSC010000144 DAHHSC010000000
VERSION     DAHHSC010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 6075)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6075)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6075
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.145"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            39..359
                     /gene="ytxJ"
                     /locus_tag="NBI57_001995"
     CDS             39..359
                     /gene="ytxJ"
                     /locus_tag="NBI57_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacillithiol system redox-active protein YtxJ"
                     /protein_id="HCD1822928.1"
                     /translation="MAIKLSSIDQFEQVIEENKYVFVLKHSETCPISANAYDQFNKFL
                     YERDMDGYYLIVQQERDLSDYIAKKTNVKHESPQAFYFVNGEMVWNRDHGDINVSSLA
                     QAEE"
     gene            783..1907
                     /locus_tag="NBI57_001996"
     CDS             783..1907
                     /locus_tag="NBI57_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HCD1822929.1"
                     /translation="MKVLVAMDEFHGIISSYQANRYVEEAVASQIETADVVQVPLFNG
                     RHELLDSVFLWQSGQKYRIPVHDADMNEVEGVYGQTDTGMTVIEGNLFLKGKKPIVER
                     TSYGLGEMIKHALDNDAKHVVISLGGIDSFDAGAGMLQALGAQFYDDEGRVVDMRQGA
                     GVIKYIRRMDMSNLHPKMETARIQVMSDFSSRLYGKQSEIMQTYDAHQLHHNQAAEID
                     NLIWYFSELFKSELKIAIGPVERGGAGGGIAAVLNGLYQAEILTSHALVDQLTHLENL
                     VEQADLIIFGEGLNENDQLLETTTLRIAELCHKHQKVAIAICATAEKFDLFESQGVTA
                     MFNTFIDMPETYTDFKMGLQIRHYTVQSLKLLKTHFNVEV"
     gene            complement(2094..3320)
                     /gene="pepT"
                     /locus_tag="NBI57_001997"
     CDS             complement(2094..3320)
                     /gene="pepT"
                     /locus_tag="NBI57_001997"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HCD1822930.1"
                     /translation="MKNQLIDRLTRYTTIDTQSDPKSTTTPSTEKQWDLLHLLEKELQ
                     QLGLPTDLDENGYLFATLESNIDADVPTVGFLAHVDTSPDFNASNVKPQIIENYDGKP
                     YKLGNTKRVLDPKVFPELNSLVGHTLMVTDGTSLLGADDKAGIVEIMEAICYLQEHPE
                     IKHGTIRIGFTPDEEIGRGPHKFDVDRFNADFAYTMDGSQYGELQYESFNAAEAVITC
                     HGVNVHPGSAKNAMVNAIRLGEQFDSLLPDSEVPERTEGYEGFYHLMNFEGTVEKATL
                     QYIIRDHDKKQFELRKKRILEIRDDINAHFENYPVKVDISDQYFNMAEKILPLPHIID
                     IPKRVFAKLDIPANTEPIRGGTDGSQLSFMGLPTPNIFTGCGNFHGPYEYASIDVMEK
                     AVQVIIGIVEDIAENH"
     gene            complement(3334..3828)
                     /locus_tag="NBI57_001998"
     CDS             complement(3334..3828)
                     /locus_tag="NBI57_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HCD1822931.1"
                     /translation="MLFYLFHFTISFISTVLFSIIFNAPKRLLVACGFVGAIAWTIYQ
                     LTVDLEFGKVGASFLGSLILGLMSHTMSRRYKRPVIIFIVPGIIPLVPGGAAYQATRF
                     LVSNDYTSAVNTFLEVTLISGAIAFGILVSEILYYLYTRIKQLYGKIKGKTYKKSYNM
                     NNRV"
     gene            complement(3846..4607)
                     /locus_tag="NBI57_001999"
     CDS             complement(3846..4607)
                     /locus_tag="NBI57_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter ThrE family protein"
                     /protein_id="HCD1822932.1"
                     /translation="MPDSITIIDENKVIDVVLIAGRILLESGAETYRVEDTMNRIAHS
                     YGLHNTYSFVSSTAIIFSLNDRTSTRLIRVQERTTDLEKIALTNSLSRKISNKELTID
                     EAKSEFIHLQHASLQYSFLTNFFAAAIACGFFLFMFGGVASDCWIAVIAGGSAFLTFS
                     FVQRYIQIKFFSEFVAAAVVISIAATFTKLGIATNQDIITIASVMPLVPGILITNAIR
                     DLLAGELLAGMSRGVEAALTAFAIGAGVAIVLLII"
     gene            complement(4803..5873)
                     /locus_tag="NBI57_002000"
     CDS             complement(4803..5873)
                     /locus_tag="NBI57_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HCD1822933.1"
                     /translation="MFEAFIYNISVIVAGIYLFHRLQYSENKRMVFSKAYVTVLMTIV
                     SLLLSVYPIPYREDYLIHLTFVPLLFLGRFTNMVYTLSATVIVAIVEIVVFNNSIMYG
                     VTLIVIAAVTSAIGPFLKQNDVLSLLILNVVTIIILFGVALVSPIYTLSEVIILIPIS
                     LIITLASAITFVDIWHFFSLVNRYENEDKYDYLTGLGNVKEFDRHLNEISRKAEKEHQ
                     SIALLLIDIDGFKDVNDTYSHKSGDAVLKQMSQLLKNYVPNQFKIFRNGGEEFSVVIH
                     NYSLDQSVKLAENIRSGVEKSSFHLPNKEVIKLSVSIGVGYLTDDDPKSQRKVFKDAD
                     DMVHVAKNQGRNKVMFNPIINL"
BASE COUNT         2084 a          921 c         1059 g         2011 t
ORIGIN      
        1 ctatttaaat gtgataaata tttttagagg tgaatagagt ggctataaag ctaagttcaa
       61 ttgaccaatt tgaacaggtt attgaggaaa ataaatatgt ttttgtatta aaacatagtg
      121 aaacttgtcc aatatcggca aatgcgtacg atcaatttaa taaattttta tatgaacgcg
      181 atatggacgg ttattatttg attgtccaac aagaacgcga tttgtcagat tatattgcta
      241 aaaaaacgaa cgttaaacat gaatcacctc aagcatttta ttttgtaaat ggtgaaatgg
      301 tttggaatcg agaccacggt gatatcaatg tgtcgtcatt agcacaagca gaagaataat
      361 gaaactatag gttggaacat tttgccttac actactagac gtgaatagca caacttaaat
      421 tcgtgtgaat cagagtagtt tggctataat gatgttctga ccttttattt tatgtcacct
      481 ttagaagcag ttaagttagt acttttttac aaacatatgt ataatgtatt cgagtatttt
      541 tattgaaaat attttggaaa acgacgaatc caataagaaa atttaaacat gatttgtaag
      601 ttagtttaat aggaaatata tgataaacca aaagaagcat actgttattt actggaataa
      661 ttaataatca tgtcatgtta aatgttcgca tataatcacg agataaaatc taaaatttaa
      721 gattaatctt ttatgaataa aaaacgtatc acaacaaata ataaagtaag gtggtcaagg
      781 ttatgaaagt attagtagcc atggatgagt ttcatggaat tatttcgagt tatcaagcta
      841 atagatatgt tgaagaggca gttgcaagcc aaattgaaac tgcagatgta gttcaagtac
      901 cattgtttaa tggaagacat gaattattag attctgtatt tttatggcaa tctgggcaaa
      961 agtatcgtat accagtacat gatgcagata tgaatgaagt tgaaggtgtt tacggacaaa
     1021 ctgatacagg gatgaccgtt atcgagggga atttattttt aaaaggtaaa aaaccaattg
     1081 ttgaacgaac aagttatggt ttaggagaaa tgattaaaca tgcattagat aatgacgcaa
     1141 aacatgttgt aatttcacta ggtgggattg atagttttga tgctggtgca ggtatgttac
     1201 aagcattagg tgctcaattc tatgatgacg aagggcgtgt cgtagatatg agacaaggtg
     1261 ctggtgtaat taaatatatt cgtcgtatgg atatgtcgaa cttacaccct aaaatggaaa
     1321 cagcaagaat tcaagtaatg tcggattttt caagtcgatt atatggtaag caaagtgaaa
     1381 tcatgcaaac ttatgatgcg catcagttgc atcataatca agcagcagaa atcgataatt
     1441 taatttggta ttttagtgag ttatttaaaa gtgaattgaa aattgcaatt ggtccagttg
     1501 aacgtggtgg tgctggtggt ggaattgcag cagtcttgaa tggactgtat caagctgaaa
     1561 tattaaccag tcatgcatta gtagaccaac taacacattt agaaaattta gttgaacaag
     1621 cggatttaat tatttttgga gaaggattaa atgaaaatga tcagttgcta gaaacgacaa
     1681 cattgcgtat tgcagaactt tgtcataaac atcaaaaggt tgccattgca atttgtgcaa
     1741 ctgctgaaaa gtttgattta tttgaatcac aaggggttac agcaatgttt aatacattta
     1801 tcgatatgcc agaaacttat actgacttta aaatgggatt acaaattagg cattatacgg
     1861 ttcagtcttt aaaactgttg aaaacacatt ttaatgttga ggtttagtaa agaaggacta
     1921 aattgatgat gctgtcatga tggttaataa catttatgat ggttagcaaa acgaattaga
     1981 agatcgaaag tatacgtaaa aaatatgaaa aatcacgcta tcattgcact gaatgttagc
     2041 gtgatttttt tattaattaa gcctgagttg aactagtata taatcgtttg tttttagtga
     2101 ttttcagcga tatcttctac aattccaatg attacttgta ctgctttttc cataacatca
     2161 atggatgcat attcatatgg gccgtggaag ttaccgcaac ctgtaaagat gtttggagtt
     2221 ggtaacccca taaatgacaa ttgtgaacca tctgtaccac cacgaatagg ttcagtgttt
     2281 gctggaatat ctaatttggc aaagacacgt ttaggtatat caataatatg aggcaatggt
     2341 aatatttttt ctgccatatt gaaatattga tccgatatat caactttaac tggataattt
     2401 tcaaaatggg cattgatatc gtcacgtatt tctaaaatac gtttcttacg caattcgaat
     2461 tgttttttat catgatcacg aataatgtat tgcaaagttg ctttttcaac agttccttca
     2521 aagttcatta agtgataaaa gccttcgtat ccttctgttc gctccggaac ttcactatca
     2581 ggtagcaaac tatcgaattg ttcacctaaa cgtattgcgt ttaccattgc atttttagct
     2641 gaaccaggat gaacatttac accgtggcat gtaataaccg cttcagcagc gttaaagctt
     2701 tcatattgta attctccata ttgactacca tccatagtat aagcaaaatc agcattgaag
     2761 cggtcaacat caaatttatg tggaccacga ccgatttctt cgtctggtgt aaatccaatg
     2821 cgaatggtac catgtttaat ttctggatgt tcttgtaaat aacaaatagc ttccataatt
     2881 tccacaatac ccgctttatc gtctgcacct agtaacgatg taccatcagt taccattaat
     2941 gtatgaccaa ctaaactgtt aagttctgga aatactttag gatctaagac acgtttagta
     3001 ttgcctagtt tgtatggctt accatcatag ttttcaataa tttgcggttt aacatttgaa
     3061 gcattgaaat caggtgatgt atcaacatgc gccaaaaatc caactgttgg gacgtcggca
     3121 tcgatgttac tttctaatgt agcaaataag tagccatttt catctaaatc agttggcaat
     3181 cctaattgtt gtaattcttt ttctaataaa tgtaacaaat cccattgctt ttcagttgaa
     3241 ggtgttgttg tagattttgg atcagattgc gtatcaattg tcgtatatct tgttaatcta
     3301 tctatcaatt ggttcttcat tatattcgac cccttaaact ctattattca tgttgtaaga
     3361 ttttttatat gtcttacctt tgattttacc atacagttgt ttgatacgtg tgtataggta
     3421 atatagaatt tcagaaacta atataccgaa agcaatcgca cctgaaatca gtgtaacttc
     3481 taaaaatgta tttacagcac ttgtataatc attcgatact aaaaaacgag tcgcttgata
     3541 agctgcacca ccaggtacta atggtataat gcctggtact atgaatataa ttaccggtcg
     3601 tttatatctg cgactcatag tatgactcat taagcctaaa attaagcttc ccaaaaatga
     3661 agcgccaact tttccaaact ctaaatctac cgttaattgg taaatcgtcc atgcaatggc
     3721 acctacaaat ccacatgcta ctaagaggcg tttgggtgca ttgaaaatga tagagaaaag
     3781 tactgttgat ataaagctga ttgtaaaatg aaataaataa aatagcatgc tttaacagtc
     3841 cttccttaaa tgattaataa aacgattgcg acaccagcac cgattgcgaa tgctgttaat
     3901 gcagcttcaa cacctcgaga catacctgca agtaattcac ccgctaataa atctcgaatg
     3961 gcattggtaa ttaatatacc agggacaagt ggcatgacac tggctatagt aatgatatct
     4021 tgattggttg caatgcctaa tttagtaaat gtggctgcaa tggatatgac cacagcagct
     4081 gcaacaaact ctgagaaaaa tttaatttgt atatagcgtt gcacaaagct gaatgttaaa
     4141 aatgcggatc caccagcaat gactgcaatc caacaatctg atgcgacacc accaaacata
     4201 aataggaaga agccacatgc aatggcagct gcaaagaaat tcgttaaaaa agaatattgt
     4261 aatgatgcat gctgtaaatg aataaattca gatttagctt catcaattgt gagttcttta
     4321 tttgatattt tacgtgaaag actattcgtt aaagcgattt tctctaaatc tgttgtacgc
     4381 tcttgtacac gaattaatct tgtacttgtt cgatcgttta atgaaaaaat aattgcagtt
     4441 gaactgacaa aactatatgt attatgaaga ccataactat gtgcgatacg gttcattgta
     4501 tcttcaactc gatatgtttc agcacctgat tcaagtaaaa ttctacctgc aattaataca
     4561 acatcaatca ctttgttttc atctataatt gtgattgaat ctggcatatc aattcacctc
     4621 caatgatatg tgttatttat ttgaacaatt gaagtttaca acttgttgtt acaactttca
     4681 atagtgagac tttgtgttag tatgatgaac ttgtatggtt caaatttaaa taagaaaaac
     4741 tgttaatctt tgctattata ctatgattta ataatagcaa aggattaaca gttttgtcgt
     4801 tgttataaat tgataatagg gttaaacatt actttgtttc gcccttgatt tttggctaca
     4861 tgcaccatat cgtctgcatc tttaaacact ttacgctgtg attttggatc gtcatctgtt
     4921 aaataaccaa caccgataga cactgacaat ttaataactt ctttgtttgg taaatggaat
     4981 gatgattttt caacacccga acgaatattt tcagctaatt taacactttg atcaagtgaa
     5041 taattgtgaa tgacaactga gaactcttcg ccaccatttc taaaaatttt aaattgattc
     5101 ggcacatagt ttttaagtaa ttgagacatt tgttttaata cagcatcacc tgatttgtgt
     5161 gagtaggtat cattgacatc tttaaatcca tcgatatcga ttaataataa agcgatactt
     5221 tgatgttctt tttcagcttt tcgtgaaatt tcatttaaat gtctatcaaa ttcttttaca
     5281 ttacctaagc ctgttaagta atcatattta tcttcgtttt cataacgatt tacgagtgag
     5341 aagaaatgcc aaatatcgac aaatgttatc gctgaagcta aagtgataat taatgaaatt
     5401 ggtattaaaa tgataacttc cgatagtgta taaataggac tcactaacgc gacaccaaat
     5461 aaaatgatta ttgtaacaac attaagtatt aataatgata gcacatcatt ttgttttaaa
     5521 aatggtccaa tagcacttgt tactgcagca ataacaatca acgtaacacc gtacataatc
     5581 gagttgttaa atactacaat ttcaacaatt gctacaatta ctgtggcaga taatgtatag
     5641 accatatttg taaatctacc taaaaacaat aaaggaacga atgttaagtg aattaaataa
     5701 tcttcacgat aagggatagg gtagacagat aataataatg atacgattgt cattaaaaca
     5761 gtgacataag ccttagaaaa aaccatacgt ttgttttctg aatactgtaa gcgatggaat
     5821 aaatagattc cagcgactat aacagatata ttgtatataa atgcttcgaa catgtctgaa
     5881 tcgactcctt taattgacca ctagctattg taagtgaaaa cttacaattt gtcattagtt
     5941 tacatataaa attaatgtat gatatagact ttgatgttaa aatgttgcct taaatgatat
     6001 gatgaaaaaa tgaataatag gcgatatata agaaatgaat cgtatagttg taaatatgat
     6061 atcattgatt gagcg
//
LOCUS       DAHHSC010000145         6070 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.146, whole genome shotgun sequence.
ACCESSION   DAHHSC010000145 DAHHSC010000000
VERSION     DAHHSC010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 6070)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6070)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6070
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.146"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            complement(60..1097)
                     /gene="mutY"
                     /locus_tag="NBI57_002001"
     CDS             complement(60..1097)
                     /gene="mutY"
                     /locus_tag="NBI57_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HCD1822934.1"
                     /translation="MYQQSSFKENLIHWFDENQREMPWRQTTNPYYIWLSEVMLQQTQ
                     VKTVIDYYHRFVERFPTVEVLSQASEDEVLKYWEGLGYYSRARNFHTAIKEVYDKYEG
                     LVPKDPDQFKALKGVGPYTQAAVMSIAYNVPLATVDGNVFRVWSRLNDDYRDIKLQST
                     RKSYEQELLPYVTTEAGTFNQAMMELGALICTPKNPLCLFCPVQENCEAFDKGTFEKL
                     PVKSKNVSKKVIEQSVFLIRNNQGQYLLQKRSEKLLHGMWQFPMFESEHARRKMTEKI
                     GHDIQPVETPIFELKHQFTHLTWKIKVYAASGAINIETLPDDMIWFNLSDRDQYTFPV
                     PMSKIYQFING"
     gene            1249..2226
                     /locus_tag="NBI57_002002"
     CDS             1249..2226
                     /locus_tag="NBI57_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HCD1822935.1"
                     /translation="MDTATHIAIGVGLTALATQDPAMASTFGATATTLIVGSLIPDGD
                     TVLKLKDNATYISHHRGITHSIPFTILWPILITFLIFTFFSGTNPFHVWMWAQLAVFL
                     HVFVDIFNSYGTQALRPITNKWIQLSVINTFDPIIFTVLCIGIVLWVIGLHPFAVFFP
                     IIALLIIYYMIRFKMRAVIKQQALKAIQQEHHPVKVFVAPTIKFMEWRVAIQTDAHDY
                     VGKAYGRNVVFSDKVERQTLSTDSILWKVKGNKDIRTFLNFSSIYRWQTTTLADGSTE
                     IRLIDLRYLKNDHYSFVAIAHVTNDNVIDHSYIGWVFTEDKLQRKLYAK"
     gene            complement(2487..3323)
                     /locus_tag="NBI57_002003"
     CDS             complement(2487..3323)
                     /locus_tag="NBI57_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1822936.1"
                     /translation="MLDNIILYFKNLPHTKRYVTERLKQSWKSFLIVLAACLILIIAS
                     ETLFSFSHLTDVKEVRWLFRIIALIVFAVLMFTIYISYHHYMNDFLVTKLFNISAATP
                     VVIMSILSFIMLVILTMISALVKPVNFETSYIALFYFIVMATIFVGLISVTFGLIRLL
                     TEKINIIFYGVCVLCILLLPIIFIPNPNHVFINHILMLNPMYYIVNGIAQSIIFGISS
                     MENIPYHFYFILFLCLIAAVNFVLARYTTHAIYNKTSKVTQTDNQQDVSNDSTDEADT
                     SS"
     gene            complement(3332..4849)
                     /locus_tag="NBI57_002004"
     CDS             complement(3332..4849)
                     /locus_tag="NBI57_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCD1822937.1"
                     /translation="MGSSIVLKLLKVTHYYRNKQNKKWYLPFGYDAEDIDLNNISLHI
                     YQGEALGIIGEPESSKALVGQLLAGAIKPDKGKVVCTEDLFYGYIEDQSLIHQTVEAY
                     TAQLVQLFPYEINDHKAEQIIQYAHLGDYKTKPVNHISKAAYAQLLLSIARSSKSNII
                     ILNHVIDYLTPQFMERAIELTNDYIENNLTIVSIGDDIDKISQVSNYIAWFSHGQLRM
                     EGSLKQVIPSFKEHERDRLSLNSKEEIENFDLDWKKNRTRIPEMTYNFKRVERYNHAK
                     PPKFLVRFWTLASGTILGLALMMLLIFNNIGIISITDFTNRATMQNENKDPYGEKLAY
                     GIAFNGSVDMQGDKQVTIPKYSVVTITGENSKNYRVTADNKTYYVSKDKLEYFNPAGL
                     YQTHSFKKLAPYMKSNYSNYYAYFNSQLHKKHSSVIKTLVPDDDNRFVASVTQQPIQL
                     LFNDNNQLYGFVYPIVDKKELKDKFNINNNIWITKVGNGYCIANLKEDKWIYIEL"
     gene            complement(4864..5178)
                     /locus_tag="NBI57_002005"
     CDS             complement(4864..5178)
                     /locus_tag="NBI57_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000435810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhH family protein"
                     /protein_id="HCD1822938.1"
                     /translation="MEQKKLSEMSEPELRHEIQLYKEKMRKAEMNGILNEYDVYQSKV
                     IVAESYLVDRKKIEIGKIYKLTDGSNQYFKVERLKGIFAWGFRFNSDEPEEGLPIALL
                     QL"
     gene            complement(5156..5974)
                     /gene="recX"
                     /locus_tag="NBI57_002006"
     CDS             complement(5156..5974)
                     /gene="recX"
                     /locus_tag="NBI57_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HCD1822939.1"
                     /translation="MPKITKIEVQKKNKERFNLFLDEQFEMGIDIDTLVKFNLKKGQQ
                     LEAADMAEIQKYDHYRIGLNKAIQYLSYKKRTEKEVIQYLQKEEISEQAISEVIEYCY
                     REKLIDHQDYAESLKNTMIRTTDKGPKIYQQKLYQLGIEPNIIEMFTELYREQQELDD
                     IIQIAEKISKTKKGPQNKVKEKVMQSLIQKGFEMETIHAVLNEMDFTQDEAVLDDLLQ
                     RDLEKIYNKNRKKYTQQKLISKTIEGLMRKGYKYDKIKAKLEESGIADGTEEIE"
BASE COUNT         1949 a         1051 c          823 g         2247 t
ORIGIN      
        1 tatacatttt taaaattaat gtcactatgt tttccgataa tattaccaat catcgaatgt
       61 tacccattta taaattgata aatctttgac ataggtacag ggaatgtata ttggtctcga
      121 tcacttaaat taaaccaaat catgtcatct ggtaatgttt caatgttaat tgctcctgaa
      181 gcggcgtata ctttaatctt ccatgttaaa tgagtaaatt gatgcttcaa ctcaaaaata
      241 ggtgtttcta ctggttgaat gtcatgaccg attttttcag tcattttacg tctagcatgc
      301 tcactttcaa acataggaaa ttgccacata ccatgcaata atttttcgct acgcttttgc
      361 aacagatatt gaccttgatt atttctaatt aaaaagacgg attgctcaat tactttttta
      421 cttacatttt tagatttaac aggtaacttt tcaaatgtac ctttatcaaa tgcctcacag
      481 ttttcttgaa ctggacaaaa taagcataat ggattttttg gtgtacaaat taatgcccct
      541 aattccatca tagcttgatt aaacgttcca gcttctgtag taacatacgg taacaattct
      601 tgttcgtacg atttcctcgt cgattgtaat ttaatatctc gatagtcatc attcaatcta
      661 gaccatactc gaaaaacatt tccgtctaca gttgctagtg gtacattata tgcaatgctc
      721 attactgcag cttgtgtgta tgggccaaca ccttttaacg ctttaaattg atcaggatct
      781 ttgggaacta agccttcata tttatcataa acttctttaa tcgccgtatg aaaatttcga
      841 gctctactat aatatcctaa gccttcccaa tactttaaca cttcatcttc cgaagcttga
      901 ctcaaaactt ccacagttgg aaatcgttca acaaaacgat gataatagtc aataactgtt
      961 ttaacttgtg tctgttgtaa catgacctca cttaaccaaa tatagtacgg attggtcgtt
     1021 tgtcgccatg gcatttctct ttgattttca tcaaaccagt gtatcaaatt ttctttaaaa
     1081 ctagactgct gatacattta taaaaccctt tcctcaccaa aattaattgt ctttactcat
     1141 aatgttttta ttgtacatta aaatcatggt tagtatgtaa gttaatttag ttatttgcga
     1201 aattggatta taatagtata tataatatta tgaaatgagt gaactgatat ggacactgca
     1261 acacatatcg caattggggt gggccttaca gcacttgcaa ctcaagatcc agcaatggct
     1321 tctacgtttg gtgcaacagc tacaaccctt atcgttggtt cattaattcc tgatggggat
     1381 actgttctta aattaaagga caatgcaaca tatatttcgc atcatagagg tatcacgcat
     1441 tccatccctt tcacaatact atggccaatt ttaattacat ttttaatatt cacgttcttt
     1501 agtggaacca acccatttca tgtatggatg tgggctcagc tcgcagtatt tttacatgtc
     1561 tttgtagata tattcaattc ttatggtaca caagcgctta gacctatcac aaacaaatgg
     1621 attcaattaa gtgtgattaa cacatttgac cctattattt tcacagttct ttgtattggt
     1681 attgtattat gggttatagg cttgcatcca tttgcagtct tctttcctat aatcgcttta
     1741 ctaatcattt attacatgat tcgttttaaa atgagagccg taattaagca acaagcttta
     1801 aaagcaattc aacaagagca tcaccctgtt aaagtatttg ttgcgccaac aataaaattt
     1861 atggaatggc gtgtcgcgat acaaactgat gcacatgact atgttggaaa agcatatggt
     1921 aggaatgtgg tgtttagtga taaagtggaa cgtcaaacat tatcaacaga ctccatttta
     1981 tggaaagtca aaggtaataa agatatacgt acttttttaa acttttcatc aatctatcgt
     2041 tggcaaacaa caacgttagc agatggttct actgaaattc gtttgattga tttgcgttat
     2101 ttaaaaaatg atcattattc atttgtggca attgcacatg taacaaacga taatgtcata
     2161 gaccactctt atattggctg ggtatttaca gaagataagt tacaacgtaa actgtatgct
     2221 aaataatttc aagttattat tcactaaagt taatctataa aaaatgaaca accgggcaga
     2281 atgaaaatca aaacgatttt tactctgtcc ggttttttaa tgtaaaacta tgaatgcttt
     2341 tacaaaatct aaaatttata ttgttgctaa caaactacct ttaatgactc gaaatatcaa
     2401 aatcagtata ggaaaacaat atctagatga tattctaatt gtttctgatt ctcacagatt
     2461 aatttacaca acaggtcagc taaacatcat gaagaagtat ccgcctcgtc tgtactatca
     2521 tttgaaacat cttgttgatt atcagtttgt gtcactttag atgttttatt ataaattgcg
     2581 tgtgtcgtat accttgctaa tacgaaattt actgcagcta ttaaacataa gaataaaata
     2641 aagtaaaaat gatatggaat gttttccata cttgatatac caaatatgat agattgtgct
     2701 ataccattaa caatgtaata cattggattc agcattagga tgtgattgat aaatacatga
     2761 tttggatttg gtatgaaaat aattggtaac aataagatac acaatacaca aaccccataa
     2821 aatatgatat ttattttttc agttaacagt cgaataagac caaaagtaac ggatattaat
     2881 cctacaaaaa tagttgccat cacaataaaa tagaatagcg ctatatatga tgtttcgaag
     2941 tttactggtt taaccaatgc actaatcatc gtcaaaatga ctagcataat aaaacttaaa
     3001 atagacataa taactactgg cgtcgctgcg gaaatattaa ataacttagt cactaaaaag
     3061 tcattcatat aatgatgata agagatataa attgtgaaca tcaataccgc aaaaacaatc
     3121 aatgcaataa ttctaaataa ccatcgcacc tctttaacat cggtcaaatg actaaaggaa
     3181 aatagcgttt cacttgcaat aataagtatt aaacatgcag ctaatacgat tagaaaactt
     3241 ttccaacttt gttttaaacg ttctgttaca taccttttag tatgcggtaa gtttttaaaa
     3301 tataaaataa tattatctag catctttaca cctacaattc aatataaatc catttgtctt
     3361 ctttcaaatt ggcaatacaa tatccattcc caactttagt aatccaaatg ttattgttaa
     3421 tattaaactt atcttttaat tcttttttat ctacaattgg ataaacaaaa ccgtataact
     3481 gattattatc attgaaaagt aattgtatcg gttgttgtgt aacggatgca acgaaacggt
     3541 tatcatcatc aggaactaaa gtttttataa ctgaactatg ctttttatgt aattgactgt
     3601 taaagtatgc atagtaatta ctataatttg atttcatata tggcgctaat tttttaaaac
     3661 tatgcgtttg atataaacct gccgggttaa aatattctaa tttatcttta ctaacatagt
     3721 aagtcttatt atcggcggta acacgataat ttttactatt ttcgccagta attgtaacta
     3781 cactatattt tggaattgtg acttgtttat ccccttgcat atccacactg ccattaaaag
     3841 caattccata agctaacttt tcgccatatg gatctttatt ttcattttgc atcgtagcac
     3901 gattcgtaaa atctgttatc gaaataattc ctatattatt gaaaatgagc aacatcatca
     3961 atgctaagcc taaaatagta cctgaggcta aagtccaaaa acgcactaaa aacttaggcg
     4021 gttttgcatg attatagcgc tcaacgcgtt tgaaattata ggtcatctct ggtattcttg
     4081 tacgattctt tttccaatct aaatcaaagt tttcaatttc ttcttttgag tttaaactta
     4141 atcgatcacg ttcatgttct ttaaaagatg gaataacttg tttaagtgac ccttccattc
     4201 ttaattgacc atgtgaaaac caagctatat agttactcac ttgtgaaatt ttatcaatat
     4261 catcaccaat tgacacaatc gttaaattat tttcaatata atcatttgtt aattcaatcg
     4321 cacgttccat aaattgtggt gttaaatagt caataacatg atttaaaata ataatatttg
     4381 attttgatga gcgtgcaata cttaatagta attgagcgta tgccgctttc gaaatatggt
     4441 taaccggctt cgttttataa tcgcctaaat gtgcatattg aataatctgt tcagctttat
     4501 gatcattaat ttcatatgga aatagttgaa ctaactgcgc tgtgtaagct tcaacagttt
     4561 gatgaattaa cgattgatct tcaatatatc cgtagaacaa atcttcagta caaactactt
     4621 tacctttatc aggtttaatt gcacctgcca acaattgacc taccaacgct ttggaagatt
     4681 caggttcacc aattatacct aatgcttctc cttgataaat atgtaaacta atattgttta
     4741 aatcgatatc ttcagcatca tatccaaaag gtaaatacca tttcttattc tgtttattcc
     4801 tatagtagtg tgttactttt agtaacttta aaacaattga acttcccatc tattttcatc
     4861 cttctataat tgtaataacg ctataggtaa gccttcttcg ggttcatcgc tattaaatct
     4921 aaacccccac gcaaaaatac cttttaatct ttctacttta aaatattgat tactgccatc
     4981 agtcagttta taaattttac caatttctat cttttttctg tctactaagt aactttcggc
     5041 aacaattact ttactttgat aaacgtcata ttcatttaaa ataccattca tctcagcttt
     5101 tctcattttc tctttataaa gttgtatttc gtgtcttaat tctggctcag acatctcact
     5161 caatttcttc tgttccatcg gcaataccac tttcttctaa tttagcttta attttatcat
     5221 atttatatcc ttttctcata aggccttcga tagtttttga aattaatttc tgttgcgtgt
     5281 acttctttcg atttttatta taaatttttt ctaaatctcg ttgtaataaa tcgtctaaaa
     5341 ccgcttcatc ttgtgtaaaa tccatttcat tcaatacagc atgaatggtt tccatttcaa
     5401 accctttttg aattaaagat tgcattactt tttctttaac cttattttgt ggtccttttt
     5461 ttgttttaga tattttttca gcaatttgga taatatcatc aagttcctgt tgttctctat
     5521 aaagttctgt aaacatttca atgatatttg gttctatacc aagttgataa agtttttgtt
     5581 gataaatttt aggtccttta tctgtcgtgc gaatcattgt attttttaaa ctttccgcat
     5641 aatcttgatg gtcgattaac ttttcgcgat aacaatattc aatcacttca gaaatcgctt
     5701 gctctgatat ctcttctttt tgtaaatatt gaataacttc tttttcagtt ctctttttat
     5761 atgataaata ttggattgct ttgtttaaac ctatgcgata atgatcatac ttttgaatct
     5821 ctgccatgtc agcagcttca agttgttgcc ctttttttaa attaaatttg actaatgtat
     5881 cgatatctat acccatttca aattgttcgt ctaaaaaaag attaaaacgt tctttgtttt
     5941 tcttttgaac ttctatttta gtaatcttcg gcactggatc accccgttac tttctcaagt
     6001 tttatacaat actcttttat tattcaataa gccacttcct atagcaaatg tttaacttta
     6061 aatatttttc
//
LOCUS       DAHHSC010000146         6002 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.147, whole genome shotgun sequence.
ACCESSION   DAHHSC010000146 DAHHSC010000000
VERSION     DAHHSC010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 6002)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6002)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6002
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.147"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            27..911
                     /locus_tag="NBI57_002007"
     CDS             27..911
                     /locus_tag="NBI57_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCD1822940.1"
                     /translation="MKIIQLEYFLAIVKYNSFTKAAQFLHISQPSLTATIKKMEADLG
                     YDLFTRSTKDIKITEKGIQFYRYASELVQQYRSTMEKMYDLSVTSEPRIKIGTLESTN
                     QWIANLIRKHHSDYPEQQYRLYEIHDKHQSIEQLLNFNIHLAITNEKITHEDIRSIPL
                     YEESYILLAPKETFKNQNWVDVENLPLILPNKNSQVRKHLDDYFNRRNIRPNVVVETD
                     RFESAVGFVHLGLGYAIIPRFYYQSFHTSNLEYKKIRPNLGRKIYINYHKKRKHSEQV
                     HTFVQQCQDYLYGLLEAL"
     gene            1153..1572
                     /locus_tag="NBI57_002008"
     CDS             1153..1572
                     /locus_tag="NBI57_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FosB1/FosB3 family fosfomycin resistance
                     bacillithiol transferase"
                     /protein_id="HCD1822941.1"
                     /translation="MLKSINHICFSVRNLNDSIHFYRDILLGKLLLTGKKTAYFELAG
                     LWIALNEEKDIPRNEIHFSYTHIAFTIDDSEFKYWHQRLKDNNVNILEGRVRDIRDRQ
                     SIYFTDPDGHKLELHTGTLENRLNYYKEAKPHMTFYK"
     gene            complement(1828..2763)
                     /locus_tag="NBI57_002009"
     CDS             complement(1828..2763)
                     /locus_tag="NBI57_002009"
                     /EC_number="3.5.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formimidoylglutamase"
                     /protein_id="HCD1822942.1"
                     /translation="MYKQGEPNLWTGRLDSETDPKKFRHFQTVTFEDLSKLEKSSMPS
                     GVGILGYAVDKGVALNKGRIGAKEGPDAIKQAFAGLPDLNQCETLVDYGNVYHDHEEL
                     IDTQKEFAMLAAKSIANHRQTFLLGGGHDIAYAQYLATRKVYPTQSIGVINIDAHFDT
                     RAEQQSTSGTSFRQILEEDENTDYLVLGIAQGGNTQSLFDYAKEKKIDYVFADELLSH
                     VSPTIKDMIERFVHEHDVIMFTICMDVIDSAFAPGVSAPAVLGLYPHTVLELAKRIIP
                     SDKVSSVSIAEMNPTYDADNRTAKLVANLVHHFLK"
     gene            3053..4312
                     /gene="sdpC"
                     /locus_tag="NBI57_002010"
     CDS             3053..4312
                     /gene="sdpC"
                     /locus_tag="NBI57_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease SdpC"
                     /protein_id="HCD1822943.1"
                     /translation="MKNNKISGFQWAMTIFVFFVITMALSIMLRDFQSIIGVKHFIFE
                     VTDLAPLIAAIICILVFKYKKVQLAGLKFSISLKVIERLLLALILPLIILIIGMYSFN
                     TFADSFILLQSTGLSVPITHILIGHILMAFVVEFGFRSYLQNIVETKMNTFFASIVVG
                     LMYSVFSANTTYGTEFAAYNFLYTFSFSMILGELIRATKGRTIYIATTFHASMTFGLI
                     FLFSEEIGDLFSIKVIAISTAIVAVGYIGLSLIIRGIAYLTTRRNLEELEPNNYLDHV
                     NDDEETNHTEAEKSSSNIKDAEKTGVATASTVGVAKNDTENTVADEPSIHEGTEKTEP
                     QHHIGNQTESNHDEDHDITSESVESAESVKQAPQSDDLTNDSNEDEIEQSLKEPATYK
                     EDRRSSVVIDAEKHIEKTEEQSSDKNK"
     gene            complement(4378..5064)
                     /locus_tag="NBI57_002011"
     CDS             complement(4378..5064)
                     /locus_tag="NBI57_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase A"
                     /protein_id="HCD1822944.1"
                     /translation="MKDVKALKLMTLNDVLSQINGDMTLGIGTGSTMELLLPQMAQLI
                     KERGYNITGVCTSNKIAFLAKELGIKICEINDVDHIDLAIDGADEVDPSLNIIKGGGG
                     ALFREKVIDEMASRFVVVVDETKIVQYLGETFKLPVEVDKFNWYHILRKIESYADIKV
                     ERRVNEDVAFITDNGNYILDCKLPKGIDPYKFHEYLIHLTGVFETGYFLDMADQVIVG
                     TQEGVKILEK"
     gene            5238..5891
                     /locus_tag="NBI57_002012"
     CDS             5238..5891
                     /locus_tag="NBI57_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HCD1822945.1"
                     /translation="MISIHAISTGKIQDLPYSSKRPIRSALDKTKISQTTWLSSTGFT
                     GDEQAYKDHGGPHKAVCGFSKHNYALYQDDLPTLPTHAMFGENLTFDYLDESDVYFGN
                     QYRLGEALIEVSEIREPCWKIQAKYNIPDLVKRMSTSGKTGFYFRVLKQGYVSPNDQL
                     YLIQEAPIEHRLSVQQLNDLYYNDRQNQDMLRYALNNPFLSPTRRDKLQKMYNRTLK"
BASE COUNT         2099 a         1073 c          855 g         1975 t
ORIGIN      
        1 acctataaat aaaaggagca aaacacatga aaattattca gttagaatac ttcttggcta
       61 tcgtgaaata taatagtttt actaaagctg cacaattttt acatattagc cagccatctt
      121 taactgctac gattaaaaaa atggaagcag atttaggtta tgacttattt acacgttcaa
      181 caaaagacat caagattacc gaaaaaggaa tacagtttta tcgttatgcg agcgaattag
      241 ttcaacaata tcgatccacg atggaaaaaa tgtatgattt aagcgttaca tcagaaccaa
      301 ggataaaaat tgggactctt gaatctacga atcaatggat tgcgaattta attcgaaagc
      361 accattccga ctaccctgaa cagcaatatc gtttatatga aatacatgac aaacatcaat
      421 ctatagagca attactgaat tttaatattc atttagctat aacaaatgaa aaaataaccc
      481 acgaagatat aagatccatt cctttatatg aggaatctta cattttatta gcacccaagg
      541 aaacatttaa aaatcaaaat tgggtagatg ttgaaaattt gccactcata ttaccaaaca
      601 aaaattctca agtgcgcaaa cacttagatg actattttaa tagaagaaat attcgtccaa
      661 atgtcgttgt agaaacagat cgattcgaat cagcagttgg atttgttcat ctcggcttag
      721 gttacgctat cattccgaga ttttattacc aatcatttca cacgtctaat ttagaatata
      781 aaaaaattcg tccaaactta ggccgaaaaa tttatatcaa ttaccataaa aaacgcaaac
      841 actccgaaca agtacataca ttcgtacaac aatgccaaga ttatttatat ggacttttag
      901 aggctcttta acttaagtta ttagagcctc ttatgcagtt gcgtagtcaa ctgtatacct
      961 ttttccttta acttaagtta ttagagcctc ttatgcagtt gctcagtcaa ctgtatacct
     1021 ttttccttta acttaagtta ttagagcctc ttatgcagtt gctcagatca tcgtataaaa
     1081 attaatgacg tcatttcaaa aatcgataca aaaataattt attataaaaa ttctaagaaa
     1141 gaagtgaagc agatgttaaa atctattaat catatatgct tttcagtcag aaatttaaac
     1201 gattcaatac atttttatag agatatttta cttgggaaat tgctattgac tggtaaaaaa
     1261 actgcttatt ttgagcttgc aggcctatgg attgctttaa atgaagaaaa agatatacca
     1321 cgtaatgaaa ttcacttttc atatacacat atagctttca ctatagatga cagcgaattt
     1381 aaatattggc atcagaggtt aaaagataat aacgtgaata ttttagaagg aagagttaga
     1441 gatattagag atagacaatc aatttacttt actgaccctg atggtcataa gctagaatta
     1501 catactggca cacttgagaa cagattaaat tattataaag aggctaaacc acatatgaca
     1561 ttttacaaat aaggcgtcat tataaaaagg cctcttgaaa tccgttaaaa ttttaattaa
     1621 ttattatata ataagagaac ttttcaaaca atacagttgt tatttttgct atttcaacaa
     1681 acataaataa gcagtaagat gactacaact taagagtctt cttactgcaa ttatttttca
     1741 aatatatcaa cgttaatata acttctatta agaaatactc acattctgcc ctgcaatgca
     1801 aatctcgtca catataaata tttttaatta ttttaaaaaa tgatgcacta aattagcaac
     1861 gagcttagca gttctattgt cagcgtcata tgttggattc atctcagcaa tactaactga
     1921 agacacctta tcacttggaa taatacgttt tgctaattca agaacagtat gtggatacaa
     1981 acctaacact gccggcgcac ttaccccagg cgcaaacgca ctatcaatga catccataca
     2041 aatcgtaaac ataatgacat catgttcatg tacaaaacgt tcaatcatat ctttaattgt
     2101 tggtgatacg tgactcaata attcatctgc aaagacataa tcaatctttt tctctttagc
     2161 ataatcaaat aaactttgcg tattaccacc ttgagcaata ccaagcacta aataatctgt
     2221 gttttcatct tcttctaaaa tttgtctaaa gctcgttcca gatgtagatt gttgttcagc
     2281 acgtgtatca aaatgcgcat caatatttat cacaccaata gattgtgttg gatagacttt
     2341 acgtgttgct aaatattgag catacgcaat atcatgtcca ccacctaata aaaatgtttg
     2401 tctatgatta gcaattgact tcgctgcaag catagcaaat tctttttgag tatcaattaa
     2461 ttcctcatga tcatgataaa catttccgta atcgactaaa gtttcacatt gattcaaatc
     2521 cggcaaacct gcaaatgctt gtttaatcgc atctggtcct tcttttgcac caatgcgccc
     2581 cttgtttaaa gcaacacctt tgtcaacagc atagcctaat ataccgaccc ctgatggcat
     2641 actactcttt tccagcttag acaaatcttc aaatgttact gtttgaaaat gtctaaattt
     2701 tttcgggtct gtttcactat ctaaccttcc agtccataaa tttggttcac cttgcttgta
     2761 cacagcattt ccccctctta tttatgtggc ttattaacaa ttaaagtata acgtatagga
     2821 aattttgaat tcaattcata gttaaatccg tatcttaaaa atacttatct acattacttt
     2881 tacccctatt ttctatgtaa taacgaatac ttagctgatt tatgttaata aaatacgtca
     2941 agactattac attttcatta atattgacat agacaattta tctctcggct tgtaatatgt
     3001 ataattgtta ctaaaagata ttttgcttgt tacctaatgg aggttacata taatgaagaa
     3061 caataaaatt tctggttttc aatgggcaat gacgattttc gtcttctttg tcattacaat
     3121 ggcgttatcc attatgctca gagatttcca gtctataatt ggtgtcaaac actttatatt
     3181 tgaagttaca gatctagcac cattaattgc tgcaatcatt tgtatactcg ttttcaaata
     3241 taaaaaggtc caacttgcag gtttaaaatt ctcaatcagc ctgaaagtaa ttgaacgtct
     3301 attgctagct ttaattttac ctttaattat tctaattatt ggtatgtaca gctttaatac
     3361 atttgcagat agctttattt tattacaatc aacaggctta tcagtaccta ttacacacat
     3421 tctgattgga catattctga tggcgttcgt agtagaattc ggattccgtt catacttaca
     3481 aaatattgtt gaaacgaaaa tgaacacgtt ctttgcaagt attgttgttg gtttgatgta
     3541 ttcagttttc tcagcaaata caacttatgg tacagaattt gctgcttata acttccttta
     3601 tacattctca ttctctatga ttcttggtga attaattaga gcgactaaag gacgtacaat
     3661 ttatattgca acgacattcc atgcttcaat gacattcgga cttattttct tgtttagcga
     3721 agaaatcggc gatctatttt caatcaaagt catcgccatt tcaacagcaa tcgttgcagt
     3781 aggatacatt ggtttaagct taattatccg aggtattgca tatttaacaa caagacgaaa
     3841 ccttgaagaa cttgagccta ataattattt agaccatgtc aatgacgatg aagaaactaa
     3901 tcatactgag gctgaaaaat cttcttcaaa tattaaagat gctgaaaaaa caggtgtagc
     3961 tactgcatca acggttggtg ttgctaaaaa tgatactgaa aatacagtgg ctgacgaacc
     4021 aagcattcat gaaggtactg aaaaaacaga acctcaacat cacataggta atcaaactga
     4081 atctaatcat gatgaagatc atgacatcac ttcggagtca gtagaatcag cagaatcagt
     4141 taaacaagca ccacaaagtg acgatttaac aaacgattca aatgaagatg aaatagagca
     4201 atcattaaaa gaacctgcga cttataaaga agacagacgt tcatcagttg taattgatgc
     4261 agaaaaacat atcgaaaaaa ctgaagaaca atcttcagat aaaaacaaat aataacaaag
     4321 cgcttgctag tacctcttaa aaagatgatg ctagcaagcg cttttctata ctatatatta
     4381 tttttctaaa attttaacac cctcttgagt gcctacaata acttgatctg ccatatctaa
     4441 gaagtatcct gtctcaaaca cacctgtcag atgaattaaa tactcatgga acttatatgg
     4501 gtcaattcct tttggtaact tacaatctaa aatataattg ccattatcag ttataaatgc
     4561 gacatcttcg tttacacgac gttctacttt tatatcagcg tatgattcaa ttttacgtaa
     4621 tatgtgatac cagttaaatt tatccacctc tactggtaac ttaaacgtct cacctaagta
     4681 ttgaactatt ttcgtttcat cgacaaccac aacaaaacgc gatgccattt catctataac
     4741 tttctctctg aacagcgcac caccgccacc tttaattata tttaaagatg gatctacttc
     4801 atcagcacca tcaattgcta agtcgatatg atcaacatca ttgatttcac atattttaat
     4861 acctaattct tttgctaaaa atgcaatttt attagaagtg catacacctg taatattgta
     4921 accacgttct ttaattagtt gcgccatttg aggtaagagt aattccattg tacttcctgt
     4981 accaattccc agcgtcatgt caccattgat ttgacttaaa acatcattta atgtcattaa
     5041 cttgagtgct ttgacatctt tcatgaaggt agcctcccat atttatgtaa tctattcaat
     5101 tcatatttta catgactcgt ataaattaac atacccttat ttctaacatt tttttaaaca
     5161 tatcaatcag aatttacaat tctaaaaatg ttttatagta caatatattt taaataatac
     5221 tcgttaagga gaatgatatg atatcaattc acgcaatttc gacaggaaaa atccaagatt
     5281 tgccttatag ctcgaaaaga ccgatacgct ctgctttaga taaaactaag atttcacaaa
     5341 caacatggtt atcttcaact ggtttcactg gtgatgaaca ggcttataaa gatcatggtg
     5401 gaccacataa agcagtttgt gggtttagta agcataatta tgcactgtat caagatgatt
     5461 tacctacact acctactcat gcgatgtttg gagagaattt aacatttgat tatttagacg
     5521 aatctgatgt ttactttggt aatcaatatc gtttaggtga agcgttaatt gaggtttctg
     5581 aaattagaga accatgctgg aaaattcaag caaaatataa tattcctgat ttagtgaagc
     5641 gcatgtctac atctggtaaa acaggtttct atttccgggt attaaaacaa ggctatgtat
     5701 ctccaaatga tcagctttac ttaatacaag aagcaccaat cgaacatcgt ttatctgtac
     5761 aacagcttaa tgacctttat tataatgata gacaaaatca agatatgtta cgatatgcac
     5821 taaacaatcc atttctgtca ccaacaagac gcgataaact tcaaaaaatg tataacagaa
     5881 cattgaaata attacctttc attataagtg ttaaatgaac ttttcaaaac aaaaaggaat
     5941 caacttacac atcgtttgta taatagtctt atctataacc actgatgcta ttgattcctt
     6001 tt
//
LOCUS       DAHHSC010000147         5938 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.148, whole genome shotgun sequence.
ACCESSION   DAHHSC010000147 DAHHSC010000000
VERSION     DAHHSC010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 5938)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5938)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5938
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.148"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            complement(140..553)
                     /locus_tag="NBI57_002013"
     CDS             complement(140..553)
                     /locus_tag="NBI57_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1822946.1"
                     /translation="MKPTKVILRDASYLHSKTSITFILKDVVIEEDNKIYYFDTSATF
                     EDQEVKFEFALFDSDMDNLKHMDYDNPVTEIYFIEPDLHFTIIDFNQELLCIYIDFDS
                     GLRHSNMATESGISLRINIAKSDFTKFINELASLY"
     gene            complement(582..693)
                     /locus_tag="NBI57_002014"
                     /pseudo
     CDS             complement(582..693)
                     /locus_tag="NBI57_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500597.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(779..1510)
                     /locus_tag="NBI57_002015"
     CDS             complement(779..1510)
                     /locus_tag="NBI57_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HCD1822947.1"
                     /translation="MHELTIYHFMSDKLNLYSDIGNIIALRQRAKKRNIKVNVVEINE
                     TEGITFDECDIFFIGGGSDREQALATKELSKIKTPLKEAIEDGMPGLTICGGYQFLGK
                     KYITPDGTELEGLGILDFYTESKTNRLTGDIVIESDTFGTIVGFENHGGRTYHDFGTL
                     GHVTFGYGNNDEDKKEGIHYKNLLGTYLHGPILPKNYEITDYLLEKACERKGIPFEPK
                     EIDNEAEIQAKQVLIDRANRQKKSR"
     gene            complement(1512..2825)
                     /gene="murT"
                     /locus_tag="NBI57_002016"
     CDS             complement(1512..2825)
                     /gene="murT"
                     /locus_tag="NBI57_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid II isoglutaminyl synthase subunit MurT"
                     /protein_id="HCD1822948.1"
                     /translation="MRQWTAIHLAKLARKASRAVGKRGTDLPGQIARKVDTDVLRKLA
                     EQVDDIVFISGTNGKTTTSNLIGHTLKANNIQIIHNNEGANMAAGITSAFIMQSTPKT
                     KIAVIEIDEGSIPRVLKEVTPSMMVFTNFFRDQMDRFGEIDIMVNNIAETISNKGIKL
                     LLNADDPFVSRLKIASDTIVYYGMKAHAHEFEQSTMNESRYCPNCGRLLQYDYIHYNQ
                     IGHYHCQCGFKREQAKYEISSFDVAPFLYLNINDEKYDMKIAGDFNAYNALAAYTVLR
                     ELGLNEQTIKNGFETYTSDNGRMQYFKKERKEAMINLAKNPAGMNASLSVGEQLEGEK
                     VYVISLNDNAADGRDTSWIYDADFEKLSKQQIEAIIVTGTRAEELQLRLKLAEVEVPI
                     IVERDIYKATAKTMDYKGFTVAIPNYTSLAPMLEQLNRSFEGGQS"
     gene            3117..3617
                     /gene="ftnA"
                     /locus_tag="NBI57_002017"
     CDS             3117..3617
                     /gene="ftnA"
                     /locus_tag="NBI57_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="H-type ferritin FtnA"
                     /protein_id="HCD1822949.1"
                     /translation="MLSKNLLEALNDQMNHEYFAAHAYMAMAAYCDKESYEGFANFFI
                     QQAKEERFHGQKIYNYINDRGAHAEFRAVSAPKIDFSSILETFKDSLSQEQEVTRRFY
                     NLSEIARQDKDYATISFLNWFLDEQVEEESMFETHINYLTRIGDDSNALYLYEKELGA
                     RTFDEE"
     gene            complement(3699..3791)
                     /locus_tag="NBI57_002018"
     CDS             complement(3699..3791)
                     /locus_tag="NBI57_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1822950.1"
                     /translation="MTKNTIISLENEKTQINDSENESSDLRKAK"
     gene            4007..4561
                     /locus_tag="NBI57_002019"
     CDS             4007..4561
                     /locus_tag="NBI57_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HCD1822951.1"
                     /translation="MIQDAFVALDFETANGKRTSICSVGMVKVIDSQITETFHTLVNP
                     QDYFSQQNIKVHGIQPEDVENAPTFDYVFPYMMQFIADLPVVAHNAAFDMNVLHQSIQ
                     NIGLPTPNLTYFCSYQLAKRTVDSYRYGLKHMMEFYQLDFHGHHDALNDAKACAMITF
                     RLLKNYENLTYVTNIYGKNLKDKG"
     gene            complement(4629..5699)
                     /gene="dinB"
                     /locus_tag="NBI57_002020"
     CDS             complement(4629..5699)
                     /gene="dinB"
                     /locus_tag="NBI57_002020"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011302771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HCD1822952.1"
                     /translation="MTERRIIHIDMDYFFAQVEMRDNPKLKGKPVIVGGKASSRGVVS
                     TASYEARKYGVHSAMPMSQAHKLCPNGYFVTSNFGAYRETSAQIMSIFRSYTDKVEPM
                     SLDEAYLDITELVRPDLPASKIAQYIRKDILEQTHLTASAGVSYNKFLAKLASGMNKP
                     DGLTVIDYQNVHDILMTLDIGDFPGVGKASKKVMHDNGIFNGRDLYEKTEFELIRLFG
                     KRGRGLYNKARGIDHSEVKSSRVRKSVGTERTFATDVNDDEEILRKVWELSGKTAERL
                     NKLQKSAKTVTVKIKTYQFETLSKQMSLRDSVSSEEDIYNIAYLLYNDLKDPDVPIRL
                     IGVTVGNLEQSTYKNMTIYDFI"
BASE COUNT         1937 a         1066 c          802 g         2133 t
ORIGIN      
        1 gcctcttttt atcgtatata gcatgtccct ttttaaaatt acaagcataa atattcagac
       61 tcaaatatag ccacacgcct aaactacgga gggatgtggc tgtctttttt gattgtaatt
      121 atgtatcaga tttaaatcat taatataaag aggctaattc attaataaat ttagtaaaat
      181 ctgatttagc aatatttatc cttaatgaaa taccagattc tgttgccatg tttgaatgcc
      241 ttaaaccaga atcaaaatca atataaatac aaagcagttc ttgattaaaa tcaataattg
      301 taaaatgtaa atcaggttct ataaaatata tttcagtaac aggattgtcg taatccatat
      361 gtttcaaatt gtccatgtca ctatcaaaaa gtgcaaattc aaatttaact tcttgatctt
      421 cgaaagttgc actagtgtcg aaataataaa ttttattatc ttcttcgata actacatctt
      481 ttaaaataaa tgttatcgat gttttgctat gtaaataaga tgcatctctt aaaatcactt
      541 tagtgggctt catcgtcaaa ttcctttcat cattcatttt ttcatgttgc ataatcacca
      601 ttactattga taattctagg atagatactc attgtataat aattattcac tagaacaaaa
      661 aaaacacaca tcaatttggt tgtttccatc caaaataatg tgtgtgttta tacatatatg
      721 atttattact aaacttcatc gtttatcaat attaaatcca ttgatgcgat gttcagagtt
      781 aacgagattt cttctgtcta tttgctctgt ctattaatac ttgtttcgct tgtatttccg
      841 cttcattatc tatttcttta ggctcaaacg gaatcccctt acgttcacaa gctttttcta
      901 acagataatc agtgatttcg taatttttag gtaaaattgg tccgtgtaaa taagtaccta
      961 ataaattttt ataatgaatg ccttcttttt tatcttcatc attattacca taaccaaaag
     1021 taacatgacc aagtgtaccg aaatcatgat atgttctacc accgtgattt tcaaaaccta
     1081 caatagttcc aaaagtatca ctttcgataa caatatctcc tgttaatcgg tttgtctttg
     1141 attcagtata aaaatctaaa atacctaacc cttctaattc tgtaccatca ggcgtgatat
     1201 atttttttcc taaaaattga tagcctccac aaattgttaa tcccggcata ccatcttcaa
     1261 tcgcttcttt aagtggtgtc ttaattttac ttaattcttt tgttgctaat gcttgttctc
     1321 tatcacttcc accaccgata aagaaaatat cacattcatc aaaggtaata ccttctgttt
     1381 cattgatttc tacgacatta actttaatat ttcgtttttt agcacgttgt cttaaagcaa
     1441 taatatttcc tatatcactg tataaattca atttatctga cataaaatga taaatagtca
     1501 attcatgcat attatgattg acctccttca aacgaacggt ttaattgttc aagcataggc
     1561 gctaatgatg tatagtttgg tattgcaact gtgaaacctt tataatccat agtctttgcc
     1621 gttgctttat aaatatcacg ctcaactata attggtactt caacctctgc taacttcaat
     1681 cgcaattgaa gttcttctgc tcgtgtacct gtcacgatga tagcttcaat ttgttgctta
     1741 gataattttt caaaatctgc atcataaatc catgaagtat ctcgaccatc tgcagcgtta
     1801 tcatttagcg aaataacata cactttttcg ccttctaatt gttcaccaac tgataaactt
     1861 gcattcattc ctgcaggatt tttagctaaa ttgatcatcg cttcttttcg ttctttttta
     1921 aagtactgca tacgaccatt gtctgatgta tacgtttcaa agccattttt aattgtttgt
     1981 tcatttaacc ctagctctct taaaacagta tatgctgcta acgcgttata agcgttaaag
     2041 tcacctgcaa ttttcatatc atatttttca tcattgatat ttaaatataa aaacggtgcc
     2101 acatcaaaac ttgatatttc atattttgct tgctctcgtt tgaaaccaca ctgacagtga
     2161 taatgaccaa tttgattata atgaatataa tcgtattgca ataagcgacc acagtttgga
     2221 caatatctac tttcattcat cgtactttgt tcaaattcat gggcatgtgc tttcatacca
     2281 tagtacacaa tcgtatcact tgcgattttc aaacgactca caaatggatc atcagcattt
     2341 agcaataatt tgatgccttt attactaatt gtctctgcaa tgttattaac cataatatca
     2401 atttcaccga agcgatccat ttgatctcta aagaaattag taaataccat cattgaaggt
     2461 gtaacttctt ttaacacacg tggaatcgaa ccttcatcaa tttcgattac cgcaatttta
     2521 gtcttaggtg ttgattgcat gatgaatgca gaagttatac ctgcagccat attagcacct
     2581 tcattattgt gtataatttg aatattattt gcttttaaag tatgtccaat taagtttgaa
     2641 gtcgttgttt taccatttgt tccactgata aatacaatat catcaacttg ctctgctaat
     2701 tttcttaata catctgtatc cacttttcta gcgatttgtc caggtaaatc tgttcctctt
     2761 ttacctactg ctctacttgc tttacgcgcc aatttcgcta gatggattgc cgtccactgt
     2821 ctcatgtgtt tcctcctcaa atttccactc gcatcattat aacatgacaa ggcaacttca
     2881 aaaaagtttc tcaatcacaa attgatacag tgtttctaaa ttataatcat tctcatttag
     2941 ataattaaaa atgatattta ttctcaatta aatatcgcta aatgatacat tttctcaatt
     3001 agagtgctaa aataaggtat taaattataa ttattattat ttacaattga gaatgagttt
     3061 gttatactaa gtttaattca tagtaatttt aatttacaaa taagaggtgt atcaaaatgt
     3121 taagtaaaaa tttattagaa gcattaaatg atcaaatgaa ccatgagtac tttgcagcac
     3181 acgcatatat ggcaatggca gcatactgtg ataaagaatc gtacgaagga tttgcaaact
     3241 tcttcattca acaagctaaa gaagaacgtt tccatggaca aaagatttat aactatatta
     3301 acgacagagg tgcacatgca gaattcagag cagtttcagc accaaaaatt gacttttcaa
     3361 gcatactaga aactttcaaa gacagcttat ctcaagaaca agaagtaaca agacgtttct
     3421 ataacttatc tgaaatcgct cgtcaagata aagattatgc aactatctca ttcttaaact
     3481 ggttcttaga tgaacaagtc gaagaagaat caatgtttga aactcacatc aattatttaa
     3541 ctcgtatcgg cgatgacagc aatgcattat atctttacga aaaagaactt ggcgctcgta
     3601 cattcgacga agaataatta aacatcacta caatagacag ataaatatca tacgacatga
     3661 taggcatttg ggtcacttac aataacccaa tgtctatatt attttgcttt acggagatca
     3721 ctagattcat tttctgaatc attgatctgc gttttttcat tttcaaggct aattattgta
     3781 tttttagtca tttatttttt aaactactaa tgttaataac tctaaatttg atgttgaatt
     3841 aatttgaccg attttaaagc atatcatcat ttacttttta atcagagtta catccaaatg
     3901 atagatttca cgttatacct tcacgtataa tattatgtat cgtttgtaag caaatgacta
     3961 aaagtctatt aatatataca tttaattaat tgaaaggatt gactacatga tacaagatgc
     4021 gtttgttgca cttgattttg aaacagcaaa tggtaaacgt acaagtattt gttctgtcgg
     4081 aatggttaaa gtcattgata gtcaaataac agaaacattt catactcttg tgaatccgca
     4141 agactatttt tcacaacaaa atattaaagt tcatggcata caaccagaag atgttgaaaa
     4201 tgcacctacg tttgactacg tatttccata tatgatgcaa tttattgcag atttacctgt
     4261 tgtcgcacat aacgcggcat ttgatatgaa cgtcttacat caaagcattc aaaatattgg
     4321 tttaccaact ccaaatttaa cttacttttg tagttatcaa cttgctaaaa gaaccgttga
     4381 ttcgtatcga tacggtttaa aacatatgat ggagttttat caattagatt ttcatggtca
     4441 tcatgatgca ttgaatgatg ccaaagcatg cgcaatgatt acttttaggc tactgaaaaa
     4501 ttatgaaaat ttaacatatg taactaatat ttatggtaaa aatctaaaag ataaaggcta
     4561 ggactaaata aaatactccc ttcaaaagta agcattgtaa aaatgtaaac tttgcaggga
     4621 gctttatttt atataaagtc atatatcgtc atatttttat aagttgattg ttctaaatta
     4681 cctacagtga caccaataag tcgaattggt acatctgggt cttttaaatc gttataaagt
     4741 aaatatgcaa tattataaat atcttcttca gaactaaccg aatctcttaa actcatctgt
     4801 ttagatagcg tttcaaattg ataagtttta attttaaccg ttacagtttt agctgacttc
     4861 tgtaatttat ttagacgttc agctgtttta cctgacaatt cccatacttt tcttaaaatc
     4921 tcttcatcat cattcacgtc tgttgcaaat gtgcgttcag tccctactga ttttcttact
     4981 cttgatgatt tcacttcact atggtcaata ccgcgtgcct tgttatataa accccgacct
     5041 ctttttccaa acaaacgtat taattcaaat tccgttttct catataaatc tctaccgtta
     5101 aaaataccat tatcatgcat tacttttttg gaagctttac ctacgcctgg aaaatctcca
     5161 atatccaatg tcatcaaaat atcatggaca ttttgataat caatcacagt caaaccatca
     5221 ggtttattca taccactcgc taatttagct aaaaatttgt tataagaaac acctgcagat
     5281 gctgttaaat gtgtctgctc tagaatatct tttctaatat actgagcaat tttcgaagca
     5341 ggaaggtctg gtctcactaa ttctgtaata tctaaatacg cttcatccaa tgacatcggt
     5401 tctaccttat ctgtataact tcggaaaata gacataatct gcgcagatgt ttctcggtaa
     5461 gcaccaaaat tacttgtgac aaagtatcca tttggacata atttatgcgc ttgtgacata
     5521 ggcattgctg aatggacgcc gtattttcgt gcttcatagg atgccgtaga gacaacaccc
     5581 ctactgcttg ctttaccacc aacaatgact ggtttccctt tcaatttggg gttatctctc
     5641 atttcgactt gtgcaaaaaa atagtccata tctatatgaa taattcgtct ctcagtcaag
     5701 tgctcacctc cctactaatt tttactttta taacgcacaa aaatatctca acataattat
     5761 acgctgtgta cgattttttt tacataaatc ttgcacttag cgataactat attgagataa
     5821 ctacaagttg ttataaaatc aattgctatt taagcatgat gatgaagacg attgagtaag
     5881 aaaacatagg taatctgaaa taattcaagc aaattcattt tgttggtatt atcatatt
//
LOCUS       DAHHSC010000148         5893 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.149, whole genome shotgun sequence.
ACCESSION   DAHHSC010000148 DAHHSC010000000
VERSION     DAHHSC010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 5893)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5893)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5893
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.149"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            72..1052
                     /locus_tag="NBI57_002021"
     CDS             72..1052
                     /locus_tag="NBI57_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HCD1822953.1"
                     /translation="MEDLERVLITGGAGFIGSHLVDDLQQDYDVYVLDNYRTGKRENI
                     KSLADDHVFELDIREYDAVEQIMKTYQFDYVIHLAALVSVAESVEKPILSQEINVVAT
                     LRLLEIIKKYNSHIKRFIFASSAAVYGDLPDLPKSDQSLILPLSPYAIDKYYGERTTL
                     NYCSLYNIPTAVVKFFNVFGPRQDPKSQYSGVISKMFDSFEHNKPFTFFGDGLQTRDF
                     VYVYDVVQSVRLIMEHKDAIGHGYNIGTGTFTNLLEVYRIIGELYGKSVEHEFKEARK
                     GDIKHSYADISNLKALGFVPKYTVETGLKDYFNFEVDNIEEVTAKEVEMS"
     gene            1015..1707
                     /locus_tag="NBI57_002022"
     CDS             1015..1707
                     /locus_tag="NBI57_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HCD1822954.1"
                     /translation="MKKLQLKKWKCRENDIEAVHNNKGYAYQRKLDALEEVRKGYYPI
                     KRAIDLVLSIVLLFLTFPIMFIFAIAIVIDSPGNPIYSQVRVGKMGKLIKIYKLRSMC
                     KNAEKNGAQWADKDDDRITNVGKFIRKTRIDELPQLINVVKGEMSFIGPRPERPEFVE
                     LFSSEVIGFEQRCLVTPGLTGLAQIQGGYDLTPQQKLKYDMKYIHKGSLMMELYISIR
                     TLMVVITGEGSR"
     gene            1917..3083
                     /locus_tag="NBI57_002023"
     CDS             1917..3083
                     /locus_tag="NBI57_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HCD1822955.1"
                     /translation="MKIIYCITKADNGGAQTHLIQLANHFCVHHDVYVIVGNHGPMIE
                     QLDARVNVIILEHLVGPIDFKQDILAVKVLAQLFSKIKPDVIHLHSSKAGTVGRIAKF
                     ISKSKDTRVVFTAHGWAFTEGVKPAKKFLYLVIEKLMSCITDSIICVSDFDKQLALKY
                     RFNRLKLTTIHNGIADVPAVKQTLKSQSHNNIGEVVGMLPNKQDLQINAPTKHQFVMI
                     ARFAYPKLPQNLIAAIEILKLHNSNHAHFTFIGDGPTLNDCQQQVVQAGLENDVTFLG
                     NVINASHLLSQYDTFILISKHEGLPISIIEAMATGLPVIASHVGGISELVADNGICMM
                     NNQPETIAKVLEKYLIDSDYIKMSNQSRKRYLECFTEEKMIKEVEDVYNGKSTQ"
     gene            3064..4302
                     /locus_tag="NBI57_002024"
     CDS             3064..4302
                     /locus_tag="NBI57_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HCD1822956.1"
                     /translation="MENQHNSKLLTLLLIGLAVFIQQSSVIAGVNVSIADFITLLILV
                     YLLFFANHLLKANHFLQFFIILYTYRMIITLCLLFFDDLIFITVKEVLASTVKYAFVV
                     IYFYLGMIIFKLGNSKKVIVTSYIISSVTIGLFCIIAGLNKSPLLMKLLYFDEIRSKG
                     LMNDPNYFAMTQIITLVLAYKYIHNYIFKVLACGILLWSLTTTGSKTAFIILIVLAIY
                     FFIKKLFSRNAVSVVSMLVIMLILLCFTFYNINYYLFQLSDLDAVPSLDRMASIFEEG
                     FASLNDSGSERSVVWINAILVIKYTLGFGVGLVDYVHIGSQINGILLVAHNTYLQIFA
                     EWGILFGALFIIFMLYLLFELFRFNISGKNVTAIVVMLTMLIYFLTVSFNNSRYVAFI
                     LGIIVFIVQYEKMERDRNEE"
     gene            4292..5722
                     /locus_tag="NBI57_002025"
     CDS             4292..5722
                     /locus_tag="NBI57_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HCD1822957.1"
                     /translation="MKSDSLKENIIYQGLYQLIRTMTPLITIPIISRAFGPSGVGIVS
                     FSFNIVQYFLMIASVGVQLYFNRVIAKSVNDKRQLSQQFWDIFVSKLFLALTVFAMYM
                     VVITIFIDDYYLIFLLQGIYIIGAALDISWFYAGTEKFKIPSLSNIVASGIVLSVVVI
                     FVKDQSDLSLYVFTIAIVTVLNQLPLFIYLKRYISFVSVNWIHVWQLFRSSLAYLLPN
                     GQLNLYTSISCVVLGLVGTYQQVGIFSNAFNILTVAIIMINTFDLVMIPRITKMSIQQ
                     SHSLTKTLANNMNIQLILTIPMVFGLIAIMPSFYLWFFGEEFASTVPLMTILAILVLI
                     IPLNMLISRQYLLIVNKIRLYNASITIGAVMNLVLCLVLIYFYGIYGAAIARLITEFI
                     LLIWRFVDITKINVKLNIVSTIQCVIAAVMMFIVLGVVNHYLPPTMYATLLLIAIGIV
                     VYLLLMMTMKNQYVWQILRHLRHKTI"
BASE COUNT         1915 a          801 c         1067 g         2110 t
ORIGIN      
        1 aaataaaaaa tatctataaa aagcttacaa tactttatgt ttttatgata tatttttaat
       61 gtataaatga ggtggaagat ttggaaagag ttttgataac tggtggggct ggttttattg
      121 ggtcgcattt agtagatgat ttacaacaag attatgatgt ttatgttcta gataactata
      181 gaacaggtaa acgagaaaat attaaaagtt tggctgacga tcatgtgttt gaattagata
      241 ttcgtgaata tgatgcagtt gaacaaatca tgaagacata tcaatttgat tatgttattc
      301 atttagcagc attagttagt gttgctgagt cggttgagaa acctatctta tctcaagaaa
      361 taaacgtcgt agcaacatta agattgttag aaatcattaa aaaatataat agtcatataa
      421 aacgttttat ctttgcttcg tcagcagctg tttatggtga tcttcctgat ttgcctaaaa
      481 gtgatcaatc attaatctta ccattatcac catatgcaat agataaatat tacggcgaac
      541 ggacgacatt aaattattgc tcgttatata acataccaac agcggttgtt aaatttttta
      601 atgtatttgg gccaagacag gatcctaagt cacaatattc aggtgttatt tcaaagatgt
      661 tcgattcatt tgagcataac aagccattta cattttttgg tgacggactg caaactagag
      721 attttgtata tgtatatgat gttgttcaat ctgtacgctt aattatggaa cacaaagatg
      781 caattggaca cggttataac attggtacag gcacttttac taatttatta gaggtttatc
      841 gtattattgg tgaattatat ggaaaatcag tcgagcatga atttaaagaa gcacgaaaag
      901 gagatattaa gcattcttat gcagatattt ctaacttaaa ggcattagga tttgttccta
      961 aatatacagt agaaacaggt ttaaaggatt actttaattt tgaggtagat aatattgaag
     1021 aagttacagc taaagaagtg gaaatgtcgt gaaaatgaca ttgaagctgt ccataataat
     1081 aagggttatg cctatcaaag aaaattagat gcactcgaag aagtgagaaa aggctattac
     1141 ccaattaaac gtgcgattga cttagtatta agtatcgttt tattattttt aacatttccg
     1201 attatgttca tattcgccat tgctatcgtc atagattcgc caggaaaccc tatttatagt
     1261 caggttagag ttgggaagat gggtaaatta attaaaatat acaaattacg ttcgatgtgt
     1321 aaaaacgcag agaaaaatgg tgcgcaatgg gctgataaag atgatgatcg tataacaaat
     1381 gtcgggaagt ttattcgtaa aacacgcatt gatgaattac cacaactaat taatgttgtt
     1441 aaaggggaaa tgagttttat tggaccacgc ccggaacgtc cggaatttgt agaattattt
     1501 agttcagaag tgataggttt cgagcaaaga tgtcttgtta caccagggtt aacaggactt
     1561 gcgcaaattc aaggtggata tgacttaaca ccgcaacaaa aactgaaata tgacatgaaa
     1621 tatatacata aaggtagttt aatgatggaa ctatatatat caattagaac attgatggtt
     1681 gttattacag gggaaggctc aaggtagtct taatttactt aataagttca aataaaagtt
     1741 atattttaaa gattgtgacc aattgttaca gtataacgag gaatcccttg agacagtatc
     1801 aaatggcatt aagaaatatg tgccatcatt gatttgcatg gctataaata ctattcatct
     1861 gatgagatag ccatgttaag aaattgaaag tatagcatta aaggggtttg taacagttga
     1921 aaattatata ttgtattact aaagcagaca atggtggtgc acaaacacat ctcattcaac
     1981 tcgccaacca tttttgcgta caccatgatg tttatgtcat tgtaggcaat catggaccaa
     2041 tgattgaaca actagatgca agagttaatg taattattct cgaacattta gtaggtccaa
     2101 ttgactttaa acaagatatt ttagctgtca aagtgttagc acagttattc tcgaaaatta
     2161 agcctgatgt tatccattta cattcttcca aagctggaac ggtcggacga attgcgaagt
     2221 tcatttcgaa atcgaaagac acacgtgtag tttttactgc gcatggatgg gcttttacag
     2281 agggtgttaa accagctaaa aaatttctat atctagttat cgaaaaatta atgtcatgta
     2341 ttacagatag cattatttgt gtttcagatt tcgataaaca gttagcgtta aaatatcgat
     2401 ttaatcgatt gaaattaacc acaatacata atggtattgc agatgttccc gctgttaagc
     2461 aaacgctaaa aagccaatca cataacaata ttggcgaagt agttggaatg ttgcctaata
     2521 aacaagattt acagattaat gccccgacaa agcatcaatt tgttatgatt gcaagatttg
     2581 cttatccaaa attgccacaa aatctaatcg cggcaataga gatattgaaa ttacataaca
     2641 gtaatcatgc gcattttaca tttataggcg atggacctac attaaatgat tgtcagcaac
     2701 aagttgtaca agctgggtta gaaaatgatg tcacattttt gggcaatgtc attaatgcga
     2761 gtcatttatt atcacaatac gatacgttta ttttaataag taagcatgaa ggtttgccaa
     2821 ttagcattat agaagctatg gctacaggtt tgcctgttat agccagtcat gttggcggta
     2881 tttcagaatt agtagctgat aatggtatat gtatgatgaa caaccaaccc gaaactattg
     2941 ctaaagtcct ggaaaaatat ttaatagaca gtgattacat caaaatgagt aatcaatcta
     3001 gaaaacgtta tttagaatgt tttactgagg agaaaatgat taaagaagtg gaagacgttt
     3061 ataatggaaa atcaacacaa tagtaaatta ctaacattgt tacttatcgg tttagcggtt
     3121 tttattcagc aatcttcggt tattgccggt gtgaatgttt ctatagctga ctttatcaca
     3181 ttactaatat tagtttattt actgtttttc gctaaccatt tattaaaggc aaatcatttt
     3241 ttacagtttt tcattatttt gtatacatat cgtatgatta ttacgctttg tttgctattt
     3301 tttgatgatt tgatatttat tacggttaag gaagttcttg catctacagt taaatatgca
     3361 tttgtagtca tttatttcta tttagggatg atcatcttta agttaggtaa tagcaaaaaa
     3421 gtgatcgtta cctcttatat tataagcagt gtgactatag gtctattttg tattatagct
     3481 ggtttgaaca agtccccttt actaatgaaa ttgttatatt ttgatgaaat acgttcaaaa
     3541 ggattaatga atgaccctaa ctatttcgcg atgacacaga ttattacatt ggtacttgct
     3601 tacaagtata ttcataatta catattcaag gtccttgcat gtggtatttt gctatggtct
     3661 ttaactacaa cggggtctaa gactgcgttt atcatattaa tcgtcttagc catttatttc
     3721 tttattaaaa agttatttag tagaaatgcg gtaagtgttg tgagtatgtt agtgattatg
     3781 ctgatattac tttgttttac cttttataat atcaactact atttattcca attaagcgac
     3841 cttgatgccg taccgtcatt agatcgaatg gcgtctattt ttgaagaggg ctttgcatca
     3901 ttaaatgata gtgggtctga gcgaagtgtt gtatggataa atgccatttt agtaattaaa
     3961 tatacactag gttttggtgt cggattagtg gattatgtac atattggctc gcaaattaat
     4021 ggtattttac ttgttgccca taatacatat ttgcagatct ttgcggaatg gggcatttta
     4081 ttcggtgcat tatttatcat atttatgctt tatttactgt ttgaattatt tagatttaac
     4141 atttctggga aaaatgtaac agcaattgtt gtaatgttga cgatgctgat ttacttttta
     4201 acagtatcat ttaataactc aagatatgtc gcttttattt taggaattat cgtctttatt
     4261 gttcaatatg aaaagatgga aagggatcgt aatgaagagt gattcactaa aagaaaatat
     4321 tatttatcaa gggctatacc aattgattag aacgatgaca ccactgatta caatacccat
     4381 tatttcacgt gcatttggtc ccagtggtgt gggtattgtt tcattttctt tcaatatcgt
     4441 gcaatacttt ttgatgattg caagtgttgg cgttcagtta tattttaata gagttatcgc
     4501 gaagtctgtt aacgacaaac ggcaattgtc tcagcagttt tgggatatct ttgtcagtaa
     4561 attattttta gcgttaacag tttttgcgat gtatatggtc gtaattacta tatttattga
     4621 tgattactat cttattttcc tactacaagg aatctatatt ataggtgcag cactcgatat
     4681 ttcatggttt tatgctggaa ctgaaaagtt taaaattcct agcctcagta atattgttgc
     4741 gtctggtatt gtattaagtg tagttgttat ttttgtcaaa gatcaatcag atttatcatt
     4801 gtatgtattt actattgcta ttgtgacggt attaaaccaa ttacctttgt ttatctattt
     4861 aaaacgatac attagctttg tttcggttaa ttggatacac gtctggcaat tgtttcgttc
     4921 gtcattagca tacttattac caaatggaca gctcaactta tatactagta tttcttgcgt
     4981 tgttcttggt ttagtaggca cataccaaca agttggtatc ttttctaacg catttaatat
     5041 tttaacggtc gcaatcataa tgattaatac atttgatctt gtaatgattc cgcgtattac
     5101 caaaatgtct atccagcaat cacatagttt aactaaaacg ttagctaata atatgaatat
     5161 tcaattgata ttaacaatac ctatggtctt tggtttaatt gcaattatgc catcatttta
     5221 tttatggttc tttggtgagg aattcgcatc aactgtccca ttgatgacca ttttagcgat
     5281 acttgtatta atcattcctt taaatatgtt gataagcagg caatatttat taatagtgaa
     5341 taaaataaga ttatataatg cgtcaattac tattggtgcg gtaatgaatt tagtattatg
     5401 tcttgttttg atatattttt atggaattta cggtgcggct attgcacgtt taattacaga
     5461 gtttatctta cttatttggc gatttgttga tattactaaa atcaatgtga agttgaatat
     5521 tgtaagtacg attcaatgtg tcattgctgc cgttatgatg tttattgtgc ttggtgtggt
     5581 caatcattat ttgcccccta caatgtacgc tacgctgcta ttaattgcga ttggtatagt
     5641 agtttatctt ttattaatga tgactatgaa aaatcaatac gtatggcaaa tattgaggca
     5701 tcttcgacat aaaacaattt aagtatcggt aacgctatac tttagaaatt taagattaag
     5761 aagaaaaggc aatttcttat tgaaaaatgg aagttgtctt ttttaattct ctttaaaagc
     5821 gggaaacaaa agcagttaaa tgcctttttg cattcaatat taaatattat atcaatttcg
     5881 aatatttaaa ttt
//
LOCUS       DAHHSC010000149         5866 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.150, whole genome shotgun sequence.
ACCESSION   DAHHSC010000149 DAHHSC010000000
VERSION     DAHHSC010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 5866)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5866)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5866
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.150"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            103..681
                     /locus_tag="NBI57_002026"
     CDS             103..681
                     /locus_tag="NBI57_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000690624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCD1822958.1"
                     /translation="MKIGYARVSTGLQNLDLQEDQLNQYGCEKIFNDHMSGSKSKRPG
                     LDKAIEFARSGDTIVVWRLDRLGRNMEDLIILVNELNNRGVSFHSLEENITMDKSSST
                     GQLLFHLFAAFAEFERNLILERSSAGRIAARARGRYGGRPEKLNKQDLKLLKTLYDNG
                     TPIKTIAEQWKVSRTTIYRYLNKLNNQENKDK"
     gene            808..1419
                     /locus_tag="NBI57_002027"
     CDS             808..1419
                     /locus_tag="NBI57_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000583532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCD1822959.1"
                     /translation="MIIGYARVSSIDQNLERQLENLKTFGAEKIFTEKQSGKSIENRP
                     VLQEVLNFVRMGDRFVVESIDRLGRNYDEIIETVNYLKEKDVQLMITSLPMMNEVIGN
                     HLLDKFMKDLIIQILAMVSEQERNESKRRQAQGIQVAKDKGVYKGRPLLYSPNAKDPQ
                     KRVIYHRVVEMLEEGQAISKIAKEVNITRQTVYRIKNDKGLCW"
     gene            complement(1772..2191)
                     /locus_tag="NBI57_002028"
     CDS             complement(1772..2191)
                     /locus_tag="NBI57_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000418034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HCD1822960.1"
                     /translation="MELDNCINYLLSTSQNRVFKHFKSLLSPYNITPAEYGVLNCLIN
                     SNLNTPKAIGNTLNLEPSTISGILDKMSKKDLITRQTDNENRRTVLINSTNKAKDLWP
                     TLEKKAYQLNNNYFANLTNEETVVFKKILLKINETTF"
     gene            2315..3286
                     /locus_tag="NBI57_002029"
     CDS             2315..3286
                     /locus_tag="NBI57_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000481285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HCD1822961.1"
                     /translation="MFNAVVVRKSASNVSYKVETVDESYLSKGDVLVKVVYSSINYKD
                     MLALQYNGGVIRDYPMIPGIDFAGIVESSSNDKFKEGDNVLVTGYDVGVTHTGGFSEY
                     AQVPGEWIVPIPQEMSIKESMVYGTAGFTAALSIFELEKYGMNIKSQPEILVSGATGG
                     VGSVAIQILRKIGYENITAIVRKEKQIEIAKKLGAKQVIIIEDDNKPLKKGIFDYFLD
                     TVGNNVTLYGLKRLNYQGVATVCGNVAGNSLNANILPFILRGIKLIGIDSVCVDHNIR
                     EDIWSKLANEWNIGNSLLYNEIGFEEFYKTIDQLLKGQHLGRTILKV"
     gene            complement(3458..3550)
                     /locus_tag="NBI57_002030"
     CDS             complement(3458..3550)
                     /locus_tag="NBI57_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002495832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HCD1822962.1"
                     /translation="MLIIFVHIIAPVISGCAVAYYTYWLSKRNK"
     gene            3833..4612
                     /gene="ser"
                     /locus_tag="NBI57_002031"
     CDS             3833..4612
                     /gene="ser"
                     /locus_tag="NBI57_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_031888056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphylococcal enterotoxin type R"
                     /protein_id="HCD1822963.1"
                     /translation="MLNKILLLLLSVTFMLLFFSLHSVSAKPDPRPGELNRVSDYKKN
                     KGTMGNVESLYKDKAVIAENVKNTRQFLGHDLIFPIPYSEYKEVKSEFINKKTADKFK
                     DKRLDVFGIPYFYTCLVPKNESREEFIFDGVCIYGGVTMHSTADSISKNIIVPVTVDN
                     KQQFSFTISTNKKTVTVQELDYKVRNWLTNNKKLYEFDGSAYETGYIKFIEENKDSFW
                     YDLFPKKDLVPFIPYKFVNIYGDNKTIDASSVKIEVHLTTT"
     gene            complement(4702..5508)
                     /gene="sej"
                     /locus_tag="NBI57_002032"
     CDS             complement(4702..5508)
                     /gene="sej"
                     /locus_tag="NBI57_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000750881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphylococcal enterotoxin type J"
                     /protein_id="HCD1822964.1"
                     /translation="MKKTIFILIFSLTLTLLITPLVYSDSKNETIKEKNLHKKSELSS
                     ITLNNLRHIYFFNEKGISEKIMTEDQFLDYTLLFKSFFISHSQYNDLLVQFDSKETVN
                     KFKGKQVDLYGSYYGFQCSGGKPNKTACMYGGVTLHENNQLYDTKKIPINLWIDSIRT
                     VVPLDIVKTNKKKVTIQELDLQARYYLHKQYNLYNPSTFDGKIQKGLIVFHTSKEPLV
                     SYDLFNVIGQYPDKLLKIYQDNKIIESENMHIDIYLYTSLIVLISLPLVL"
     gene            complement(5665..5832)
                     /locus_tag="NBI57_002033"
     CDS             complement(5665..5832)
                     /locus_tag="NBI57_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000144396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1822965.1"
                     /translation="MTFSLLTKVAMSGLILTGAIGTAGLVSVPVANVEAKAAEFNPKV
                     DKLLKFEVSKK"
BASE COUNT         2091 a          751 c          980 g         2044 t
ORIGIN      
        1 tacatatttt agtgttcaat aacccaacag aaaatctatg ttcataaata tggttagaat
       61 taagttatga tacaatgaaa atgtatatag gaggtttgta tcttgaaaat aggttatgca
      121 agagtttcaa ctggattaca gaacttggat ttacaggaag atcagttaaa tcaatatggt
      181 tgtgaaaaga tatttaatga tcatatgagt ggttcaaaaa gtaagcgacc tggtttagat
      241 aaagccattg aatttgcgag gtcaggagat acgattgttg tttggagact agatagacta
      301 ggacgtaaca tggaagattt aatcatatta gttaatgaac ttaataatcg aggcgtgagt
      361 tttcatagtt tagaagaaaa tataacaatg gataaatcaa gttctacagg acaattgtta
      421 tttcatttat tcgcagcatt tgcagagttt gaacgtaatt taattttaga acgttcttca
      481 gcgggacgaa ttgcagctcg tgctagagga cgctatggag gtcgacccga aaaattaaat
      541 aaacaagatt taaaattact caaaacactt tatgataatg gaacaccaat taaaactatt
      601 gcagagcaat ggaaagtttc acgtacaaca atctatcgtt atctcaataa gttaaataac
      661 caagaaaata aagataaata atattactaa attttaagaa agataaaaat tagacaggat
      721 acaccctaac catacaaaaa gaattgttga aattataaca attaaaatgt atgattaggg
      781 tgtatattaa ttatggagtg aaaataagtg attataggtt atgcgagagt atcttcgata
      841 gatcaaaatt tagaaagaca actagaaaat ttaaaaacgt ttggtgctga aaaaatattt
      901 acagaaaaac agtctgggaa atctattgaa aatagaccgg tacttcaaga agttcttaat
      961 tttgtaagaa tgggagatcg attcgttgtg gagtctattg accgtttagg acgtaactat
     1021 gatgaaatta ttgaaactgt gaactattta aaagaaaaag atgtacagtt aatgattaca
     1081 agtctaccta tgatgaatga agtgattggt aatcatttac tggataaatt tatgaaagat
     1141 ttaatcattc aaatcttagc aatggtttca gaacaagaga gaaatgaaag taaacgtcga
     1201 caagctcaag gtattcaagt cgcaaaagat aaaggtgtat ataaaggacg tcctttactc
     1261 tattcaccga atgcgaaaga tcctcaaaaa cgtgttatct atcatcgagt tgtcgaaatg
     1321 ttagaagaag gacaagcaat tagtaagatt gcgaaagaag ttaatattac aagacagaca
     1381 gtttatagaa ttaaaaatga taagggatta tgttggtaaa tggattgtca ttgcaaatga
     1441 gcgtttctca cagcgtccca aaatatatgt taatcatgta ttatgagatt ttaataataa
     1501 gacaggaaaa agagagagca aatacgctct ctcttttttt ctatattttt tctcaataac
     1561 gacgatcgtt attggaattt aatttctaat acttttttat taggaatacg cctacgatat
     1621 ttttattata gaggctaaag tatcttaaaa atttggatag taggtttttc aggaataaaa
     1681 gtatagttgg ctttaatata taattaagct aagctatcat ttatatttac tcgattataa
     1741 ttcgcacttc aacgttttaa taacgtaaaa attaaaacgt agtttcattt atctttagta
     1801 atatcttttt aaacactaca gtttcttcat tagttaaatt agcgaagtaa ttattattaa
     1861 gttgataggc tttcttttct aatgttggcc ataagtcttt tgctttattt gtactgttta
     1921 ttagcacagt tcttctattt tcattatcag tttgacgtgt tattagatcc tttttggaca
     1981 tcttatctaa tattccagat attgttgaag gttctaaatt taaagtatta ccaatcgctt
     2041 taggtgtatt caaattacta ttaatcaagc aattcaaaac accatattct gctggagtaa
     2101 tattataggg acttaataaa cttttaaaat gtttaaacac tctattctga gaagtactca
     2161 acaaataatt tatacaatta tctaattcca caactaaaac ctctcttaaa tcattagtat
     2221 tgactcgctt atcatatcat gataagtttt gtaggaaaat agttttgcaa caaaaacata
     2281 cctctacaaa acaattgtga aagaaggcta ataaatgttc aatgcggtag tagtgagaaa
     2341 aagtgcatca aatgttagtt ataaagtaga aaccgttgat gaatcttatt tatcgaaggg
     2401 tgatgtctta gtaaaagtgg tatactcttc aataaattat aaggatatgc tagctttaca
     2461 atataacggc ggtgtaatta gagattatcc tatgattcct ggtatagatt ttgcaggtat
     2521 tgttgaatca agttcgaatg ataagtttaa agaaggcgat aatgtattag ttactggtta
     2581 tgatgtcggc gtgacacata caggtggatt ttcagaatat gcacaagtgc ctggagagtg
     2641 gattgttcct attccccaag aaatgtctat taaagagtct atggtatatg gcacagccgg
     2701 ttttacggca gcattatcaa tttttgaact agaaaaatat ggaatgaata tcaaatcaca
     2761 acctgaaatt ttagtgtctg gggctacagg tggtgtgggc agtgttgcta tacaaatttt
     2821 acgtaaaatt ggatatgaaa atatcactgc tatcgtaaga aaagaaaagc aaatagaaat
     2881 cgcaaaaaaa ttaggtgcaa aacaagtaat aataattgaa gatgataata aaccacttaa
     2941 aaaaggtatt tttgattatt tcctagatac agtagggaat aatgtaacat tatacggttt
     3001 aaaacgtcta aattatcaag gtgttgctac tgtatgtgga aatgtagcag gaaattcact
     3061 taacgctaat atattaccgt ttattcttag aggtattaaa ttaattggca ttgattctgt
     3121 atgtgttgat cataatatcc gtgaagatat atggagtaag ttagcaaatg aatggaatat
     3181 aggaaattca cttctataca atgaaattgg atttgaagaa ttctataaaa cgatagatca
     3241 gcttttaaaa ggacaacatt taggaagaac tattttaaaa gtataagtat gagtattcat
     3301 taatatattt actattaatt caatcatttt gcatttctct actaaccagt gttataatct
     3361 agataacgaa aatgattaac aattaatgca ccaatcccca cactattgca gtagtgtggg
     3421 gattttttcg atgctggctt ataaagtcgc ctattaatta tttgttgcgt ttactaagcc
     3481 aataagtata atacgcaaca gcacagccac tgatgactgg tgctatgatg tgaacgaaaa
     3541 tgattagcaa agtaatgcac ctcctctctg cgcctaagtt gacgactgag agataggcga
     3601 cttgtatatt atatcatctc tgaatattgg attgaatcgc ctactttctt aaattaacaa
     3661 catctattct catagaattt gtctaattaa gtgtagacga ttcagaagta tattttttga
     3721 gcttagatat tttgctaaaa tatgtttaaa ttgttaacgt ttcagcattt taaacattgc
     3781 ctaattttat aatctttatt tatgttatat taaataaaaa agaggtgtat tcatgttaaa
     3841 taaaatctta cttttattgc tctctgttac ttttatgttg ttatttttct ctttacattc
     3901 agtaagtgct aaaccagatc caagacctgg agaattgaat agagttagtg attataaaaa
     3961 aaacaaaggt actatgggga atgttgaatc cttatataag gataaagcgg taatagcaga
     4021 aaatgttaaa aatactagac aattcttagg acatgatttg atttttccta ttccttattc
     4081 agaatacaaa gaagttaaaa gtgaatttat aaataaaaaa acagctgata aatttaaaga
     4141 caaacggtta gatgtgtttg gaatacccta tttttataca tgtttagtac ctaagaatga
     4201 atcaagagaa gaatttattt ttgatggagt ttgcatatac gggggcgtta caatgcactc
     4261 cacagctgat agtatatcca aaaatataat tgttccagtt actgtagata ataaacaaca
     4321 gttctctttt acaattagta caaataaaaa aacggttacg gtacaagagc tagattataa
     4381 agtaaggaat tggttgacta acaataaaaa attatatgaa tttgatgggt cggcatatga
     4441 gacgggttat atcaaattta ttgaagaaaa taaagatagt ttttggtacg atttgtttcc
     4501 taaaaaggat ttggttccct ttatacctta taaatttgta aatatttatg gagataataa
     4561 aactatagat gcttcgagtg ttaagataga agttcacctg actacaacgt gattcatacg
     4621 gttgatactt ttgatacttt tgatagatgt actacgtata tgattttttt atagtaggtt
     4681 ctgctcttat ttttaataag tctacagaac caaaggtaga cttattaata caattaggct
     4741 tgtatataaa tatatatcga tatgcatgtt ttcagattct atgattttat tatcttggta
     4801 tatttttaat aatttgtctg gatattgacc tataacatta aataaatcat aactaaccaa
     4861 cggttctttt gaggtatgaa acacgattag tcctttctga attttaccat caaaggtact
     4921 agggttgtat aaattatatt gtttatgtaa ataatatctt gcctgaagat ctagttcttg
     4981 aatggttact tttttcttat ttgttttaac tatatctagc ggaacaacag ttctgatgct
     5041 atcaatccat aaattaatag gtattttttt agtatcataa agttgattgt tttcatgcag
     5101 tgttactcca ccatacatac atgctgtttt atttggttta ccaccagaac attgaaaacc
     5161 ataataagat ccgtatagat ctacttgttt gcctttaaac ttattaactg tttcttttga
     5221 atcgaattgt accaataagt cgttatactg tgaatgactt atgaaaaaac ttttaaataa
     5281 tagtgtgtaa tctaaaaatt gatcttctgt cattatcttt tcagagatac ccttttcgtt
     5341 aaagaagtat atatgtctga ggttatttaa agtaatacta cttaactctg attttttgtg
     5401 caaattcttt tctttaattg tttcattttt gctatcgctg taaactaaag gggttattag
     5461 tagtgttaac gtcagggaga aaatcagtat aaatattgtt tttttcattg tgttaactcc
     5521 ttaaatataa gttagctgca tttagtaatg ctggctgatt cttttatcat gcaagacttt
     5581 aaaaaatagg atgttgaaat attgaagata gaaataaata ttatacttaa tcaaattact
     5641 tacaattaag aatattttaa gagattactt tttagaaact tcaaatttta ataatttgtc
     5701 tactttggga ttgaactctg ctgctttagc ctcaacattg gcaacaggca cactaactaa
     5761 tccggctgtt cctatggcac cagttaatat taatccagac atagctactt ttgttaataa
     5821 agaaaaagtc atagttatcc ttctccattc ttttattatt ttttaa
//
LOCUS       DAHHSC010000150         5854 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.151, whole genome shotgun sequence.
ACCESSION   DAHHSC010000150 DAHHSC010000000
VERSION     DAHHSC010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 5854)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5854)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5854
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.151"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            105..791
                     /gene="mtnN"
                     /locus_tag="NBI57_002034"
     CDS             105..791
                     /gene="mtnN"
                     /locus_tag="NBI57_002034"
                     /EC_number="3.2.2.16"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/S-adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HCD1822966.1"
                     /translation="MIGIIGAMEEEVTILKNKLTQLSEISVAHVKFYTGILKDREVVI
                     TQSGIGKVNAAISTTLLINKFKPDVIINTGSAGALDESLNVGDVLISDDVKYHDADAT
                     AFGYEYGQIPQMPVAFQSSKPLIEKVSQVVQQQQLTAKVGLIVSGDSFIGSVEQRQKI
                     KKAFPNAMAVEMEATAIAQTCYQFNVPFVVVRAVSDLANGEAEMSFEAFLEKAAVSSS
                     QTVEALVSQL"
     gene            811..1338
                     /locus_tag="NBI57_002035"
     CDS             811..1338
                     /locus_tag="NBI57_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HCD1822967.1"
                     /translation="MGLVRKFFMPNSYVQSIFQIDLDKLVDKGVKGIITDLDNTLVGW
                     DVKEPTERVKAWFKEANEKGITITIVSNNNESRVASFSQHLDIDFIFKARKPMGKAFD
                     KAITKMNIRPDQTVVIGDQMLTDVFGGNRRGLYTIMVVPVKRTDGFITKVNRLIERRL
                     LRHFSKKGYITWEEN"
     gene            1339..2439
                     /gene="yqeH"
                     /locus_tag="NBI57_002036"
     CDS             1339..2439
                     /gene="yqeH"
                     /locus_tag="NBI57_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HCD1822968.1"
                     /translation="MSDILKCIGCGAPLQSEDKNKPGFVPEHNMFRDDVICRRCFRLK
                     NYNEVQDVGLESEDFLKLLSGLADKKGIVVNVVDVFDFEGSFINAVKRIVGNKKIILA
                     ANKLDLLPKQINKRRVKEWLKRTARKYGLEADDVVLISAEKGWGIDDLLSSIANIREN
                     EDVYIVGTTNVGKSTLINKLIEASVGEKDVVTTSRFPGTTLDMIDIPLDESSFMYDTP
                     GIIQDHQMTHLVSEKELKIIMPKKEIKQRVYQLNEAQTLFFGGLARIDYVSGGKRPLV
                     CFFSNDLNIHRTKTEKANDLWRNQLGDLLTPPGNPQNFDLNELKAVRLETGKEKRDVM
                     ISGLGFITIGPGAKVIVRVPKNVDVVLRNSIL"
     gene            2453..3259
                     /gene="aroE"
                     /locus_tag="NBI57_002037"
     CDS             2453..3259
                     /gene="aroE"
                     /locus_tag="NBI57_002037"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HCD1822969.1"
                     /translation="MKFAVIGNPISHSLSPVMHRANFNSLGLDDTYEALNIPIEDFHL
                     IKEIISKKELDGFNITIPHKERIIPYLDYVDEQAINAGAVNTVLIKDGKWIGYNTDGI
                     GYVKGLHSVYPDLENAYILILGAGGASKGIAYELAKFVKPKLTVANRTMARFESWNLN
                     INQISLADAEKYLAEFDIVINTTPAGMAGNNESIINLKHLSPNTLMSDIVYIPYKTPI
                     LEEAERKGNHIYNGLDMFVYQGAESFKIWTNKDADINSMKTAVLQQLKGE"
     gene            3263..3553
                     /gene="yhbY"
                     /locus_tag="NBI57_002038"
     CDS             3263..3553
                     /gene="yhbY"
                     /locus_tag="NBI57_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HCD1822970.1"
                     /translation="MLTGKQKRYLRSLAHNIDPIFQIGKGGINENMIKQIDDTLENRE
                     LIKVHVLQNNFDDKKELAETLSEATRSELVQVIGSMIVIYRESKENKEIELP"
     gene            3556..4125
                     /gene="nadD"
                     /locus_tag="NBI57_002039"
     CDS             3556..4125
                     /gene="nadD"
                     /locus_tag="NBI57_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate (nicotinamide) nucleotide
                     adenylyltransferase"
                     /protein_id="HCD1822971.1"
                     /translation="MKKIVLYGGQFNPIHTAHMIVASEVFHELQPDEFYFLPSFMSPL
                     KKHNNFIDVQHRLTMIQMIIDELGFGDICDDEIKRGGQSYTYDTIKAFKEQHKDSELY
                     FVIGTDQYNQLEKWYQIEYLKEMVTFVVVNRDKNSQNVENAMIAIQIPRVDISSTMIR
                     QRVSEGKSIQVLVPKSVENYIKGEGLYEH"
     gene            4115..4699
                     /gene="yqeK"
                     /locus_tag="NBI57_002040"
     CDS             4115..4699
                     /gene="yqeK"
                     /locus_tag="NBI57_002040"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HCD1822972.1"
                     /translation="MNIEKAKRLAKEKLPEKRYNHSLRVAETAIKLAEIYDGDTSKVE
                     LAGVLHDFCKYDDLGKMYQIVRQYELGNDLLSYGSEILHGPVCAAIMEHEYGINDEEV
                     LMAIKYHTTGRQQMTKTEKLIFIADYIEPGRTIPGVDDIRDMAYNQGSLDKTIYEISK
                     RTVLFLIQKDITVYNKTIDCLNYYNYSDERIKDD"
     gene            4700..5053
                     /gene="rsfS"
                     /locus_tag="NBI57_002041"
     CDS             4700..5053
                     /gene="rsfS"
                     /locus_tag="NBI57_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HCD1822973.1"
                     /translation="MNSQELLAIAVDAIDNKKGEDTISLEMKGISDMTDYFVVTHGNN
                     ERQVQAIARAVKEVANEQNIEVKRMEGYNEARWILIDLADVVVHVFHKDERNYYNIEK
                     LYQDAPLESYGQVAY"
     gene            5056..5772
                     /locus_tag="NBI57_002042"
     CDS             5056..5772
                     /locus_tag="NBI57_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCD1822974.1"
                     /translation="MSQYAEMSLVYDQLTQDQPYEKWFEIVKNHCKDESNILDIGCGT
                     GSLTVQLEALGNVTGMDLSVDMLTVAAQKSANVNWLEGDMTSFDLQQQFDIITIFCDS
                     LNYLQDETAVIETFINVYHHLTDSGVFIFDVHTVYKMMTLFNNQSYIDDKGDIFLAWD
                     AVQGDLPLSVYHDMTFFIRHEDETYSRFDESHFQRTFDEKTYLSWLAQVGFKHVETFT
                     DFNIDEHNEDTERLFFIAKK"
BASE COUNT         2142 a          730 c         1156 g         1826 t
ORIGIN      
        1 aaaagtttta tacagtaatg aaaatcatga tacttgtcgt tgtagtgatt aaaatgtgat
       61 aaaataacaa atgctaggat tttttaaaag aggtgaatca aaggatgatt ggtataattg
      121 gtgccatgga agaagaagta acaatattaa aaaataaatt aacacaatta agcgaaattt
      181 cagttgcaca tgttaaattt tatactggca ttttaaaaga tagagaagta gtgattaccc
      241 aaagtggcat tggaaaagtt aatgctgcaa tttctacgac attattaatt aataagttta
      301 aaccggacgt cattattaat acaggttctg ctggagcttt agatgaaagt ttaaatgtag
      361 gtgacgttct tataagtgat gatgtaaaat atcatgatgc ggatgcaaca gcatttggtt
      421 atgaatatgg acaaatacca cagatgccgg tagcatttca atcaagtaaa cctttaatag
      481 aaaaagtatc tcaagttgta caacaacaac aattaacagc taaagtaggc ttaattgtaa
      541 gtggtgatag ctttatcggt agtgttgaac aacgccaaaa aattaaaaaa gcatttccaa
      601 atgcgatggc ggttgaaatg gaagcaactg caattgcaca aacatgttat caatttaatg
      661 taccatttgt tgtagttcgt gcagtttcag acttagcaaa tggagaagcg gaaatgagct
      721 tcgaagcatt tttagaaaaa gcagctgtat catcaagtca aactgttgaa gcattagtgt
      781 ctcaattata aaaaggtaaa ggtgagttaa atgggtttag ttcgcaagtt ttttatgccg
      841 aattcatatg ttcaatcaat atttcaaatt gatttagaca agttagtgga caaaggcgtt
      901 aaaggtatta ttacagattt agataatacg ctagtaggtt gggatgttaa agaacctaca
      961 gaacgtgtta aagcatggtt taaggaagct aatgaaaaag gaatcactat tacaatcgtg
     1021 tctaataata atgagtctcg tgttgctagt tttagtcagc atttagacat cgattttatt
     1081 tttaaagcga gaaagccaat ggggaaagcg tttgataaag caataactaa gatgaatatc
     1141 agaccagatc aaactgttgt tataggtgac caaatgctta ctgatgtatt tggtggtaat
     1201 cgtcgaggtc tatatacaat tatggttgtt ccagttaaac gaactgatgg ctttattact
     1261 aaggttaata gattaattga aagacgatta ttacgtcatt tcagtaaaaa aggttatatc
     1321 acatgggagg aaaattgatt gtctgacatt ttaaaatgta tcggttgtgg tgcgccactt
     1381 caatctgaag ataaaaataa acctggtttt gtaccagagc ataatatgtt tcgtgatgac
     1441 gtgatttgca gacgttgttt ccgcttgaaa aattataacg aagttcaaga tgtaggatta
     1501 gaaagtgaag actttttaaa attattatca ggacttgcgg ataaaaaggg tattgtcgtc
     1561 aatgtcgtgg atgtatttga ctttgaagga tcatttatta atgcagttaa acgtattgtc
     1621 ggaaacaaaa aaatcatttt agcagcaaat aaattggatt tattgccgaa acaaattaat
     1681 aaacgtcgag ttaaagaatg gttaaaacga acagcaagaa aatatggttt ggaagctgac
     1741 gatgtcgtat taatttcagc tgaaaaaggc tggggcatag acgacttatt atcatcaatt
     1801 gcgaatattc gagaaaatga agatgtgtat attgtaggga caacgaatgt tgggaaatct
     1861 acattgatta ataaactgat tgaagctagt gttggtgaaa aagatgtagt aacaacttca
     1921 agattccctg gaacaacttt agatatgata gatattcctt tagatgaatc atcatttatg
     1981 tatgatacac caggtattat tcaagatcat caaatgacgc atttagttag tgaaaaagaa
     2041 ttgaaaatta ttatgcctaa gaaagaaata aaacaacgcg tatatcaatt aaatgaggcg
     2101 cagacattat tcttcggcgg tctagcgcgc atagattatg tatcaggtgg taaacgtccg
     2161 ttagtttgtt tcttttctaa tgacttgaat atacatcgta ctaaaacgga gaaggctaat
     2221 gatttatggc gtaatcaact tggcgattta ttaacgccac ctggaaatcc acaaaatttt
     2281 gatcttaatg agttaaaggc tgttagactt gaaacaggca aagagaaacg tgatgttatg
     2341 atctctggtc taggctttat aactatagga ccaggagcta aagtaatcgt tcgtgttcct
     2401 aaaaatgttg atgttgtatt aagaaattct attttataag gtgatttaaa aaatgaaatt
     2461 tgcagttatc ggaaatccta tttcacattc cttgtcgccc gttatgcata gagcaaattt
     2521 taattcttta ggattagatg atacttatga agctttaaat attccaattg aagattttca
     2581 tttaattaaa gaaattattt cgaaaaaaga attagatggc tttaatatca caattcctca
     2641 taaagaacgt atcataccgt atttagatta tgttgatgaa caagcgatta atgcaggtgc
     2701 agttaacact gttttgataa aagatggcaa gtggataggg tataatacag atggtattgg
     2761 ttatgttaaa ggattgcaca gcgtttatcc agatttagaa aatgcataca ttttaatttt
     2821 gggcgcaggt ggtgcaagta aaggtattgc ttatgaatta gcaaaatttg taaagcccaa
     2881 attaactgtt gcgaatagaa cgatggctcg ttttgaatct tggaatttaa atataaacca
     2941 aatttcattg gcagatgctg aaaagtattt agctgaattc gatatcgtta ttaatacaac
     3001 accagcgggt atggctggaa ataacgaaag tattattaat ttaaaacatc tttctcccaa
     3061 tactttaatg agtgatattg tttatatacc gtataaaaca cctattttag aggaagcaga
     3121 gcgcaaggga aaccatattt ataatggctt agatatgttt gtttaccaag gtgcggaaag
     3181 ctttaaaatt tggactaata aagatgctga tattaattct atgaaaacag cagttttaca
     3241 acaattaaaa ggagaataac atatgcttac tggcaaacaa aaaagatact taagaagttt
     3301 agcacacaat attgatccga tttttcaaat tggaaaaggc ggtatcaacg aaaatatgat
     3361 taaacaaata gatgatacgt tagaaaacag agaattgatt aaagtacatg tactacaaaa
     3421 taactttgat gataaaaaag aattagctga aacattaagc gaagctacgc gtagtgaatt
     3481 agtgcaagtg attggatcta tgatagtgat ttatagagaa tctaaagaaa ataaagaaat
     3541 tgaattgcca taataatgaa aaagatagta ctttacggcg gtcagtttaa ccctatccat
     3601 actgcacata tgatagtagc tagcgaagta tttcatgaat tacagccaga tgaattttat
     3661 tttttaccta gttttatgtc tccattgaaa aagcacaata attttataga tgttcagcac
     3721 agattaacaa tgatacagat gattatcgac gagcttggtt ttggagatat ttgcgacgat
     3781 gaaattaaac gtggtggtca gagttatacc tatgacacga tcaaggcatt caaggagcaa
     3841 cacaaagaca gtgagttgta ctttgttatt gggacggatc agtataacca actagagaaa
     3901 tggtatcaaa ttgaatactt aaaagaaatg gttacttttg tagttgtaaa tcgagacaaa
     3961 aatagtcaaa atgttgaaaa tgctatgatt gcaattcaga tacctagggt agatataagt
     4021 tcgacaatga ttcgacaaag agttagtgaa gggaaatcta tccaagttct tgttcctaaa
     4081 tccgttgaaa actatattaa gggggaagga ttatatgaac attgaaaaag caaaacggct
     4141 tgcaaaagag aaactgccag agaaacgtta taatcattct ttaagagttg cagaaacagc
     4201 gattaaattg gcagaaattt atgatggaga cactagtaaa gtagaattag caggtgtatt
     4261 acatgatttc tgtaaatatg atgatttagg taaaatgtat caaattgttc gacaatacga
     4321 attaggtaat gatctactga gttatggtag tgaaatattg catggccctg tgtgtgcagc
     4381 aatcatggaa catgaatatg gtatcaatga tgaggaagta ttaatggcta tcaaatacca
     4441 tactactgga cgtcaacaaa tgacaaaaac tgaaaaactg atttttattg ctgattacat
     4501 cgaacctgga agaacaatcc caggagttga tgatattcga gatatggcat acaatcaagg
     4561 tagtttagat aagacaattt atgaaatttc taaacgcaca gtactatttt taatacagaa
     4621 agatattacg gtatataata agacgattga ctgtttaaat tattataact atagtgacga
     4681 aagaataaag gatgattaaa tgaattcaca agaattatta gcaattgctg tggatgcaat
     4741 tgacaataaa aaaggcgaag atacgatttc tttagaaatg aaaggtatca gcgatatgac
     4801 agattatttt gttgtaacgc acggaaataa tgaacgacaa gttcaagcga ttgctagagc
     4861 ggtgaaagaa gtagccaatg aacaaaatat agaagtaaaa cgtatggaag gatacaatga
     4921 agcgcgttgg atattaattg acttagctga tgttgtggta catgttttcc ataaagacga
     4981 aagaaattat tataatattg aaaagttata tcaagatgca ccattagaat catatggtca
     5041 ggttgcgtat taattatgtc gcaatatgca gaaatgagcc tagtgtacga tcaattgact
     5101 caagatcaac catatgaaaa atggtttgaa attgtaaaaa atcactgcaa agatgaatca
     5161 aatattttag atattggatg cggtactggt agtttaacag ttcaattaga agctttaggt
     5221 aatgttactg gtatggattt aagtgttgat atgttgactg ttgcagctca aaaatcagca
     5281 aatgtaaatt ggcttgaagg agatatgaca tcttttgatt tgcaacaaca atttgatatc
     5341 atcactattt tttgtgactc tctaaattat ttgcaagatg aaactgcagt gattgaaaca
     5401 ttcatcaatg tttatcatca tctgactgat tcaggtgtat ttatttttga tgtacatact
     5461 gtttataaaa tgatgacttt gtttaataat caaagttata ttgatgataa aggggacatt
     5521 tttttagcat gggatgctgt gcaaggagat ttacctttaa gtgtttatca tgatatgaca
     5581 tttttcattc gacatgaaga tgaaacatat tcaagatttg atgagtctca ttttcagaga
     5641 acatttgatg aaaagacata tttgtcttgg ttagctcaag tcggttttaa acatgttgaa
     5701 acatttactg attttaatat agatgaacat aatgaagata cagaaagatt gtttttcatt
     5761 gcgaaaaaat aaaagtaaaa ccactcgctc agacgtgtat aaagttgagg agtggtttta
     5821 ttgtatcaat ttttattacg ctataaagat tttt
//
LOCUS       DAHHSC010000151         5733 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.152, whole genome shotgun sequence.
ACCESSION   DAHHSC010000151 DAHHSC010000000
VERSION     DAHHSC010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 5733)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5733)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5733
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.152"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            <1..1054
                     /locus_tag="NBI57_002043"
     CDS             <1..1054
                     /locus_tag="NBI57_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="undecaprenyl/decaprenyl-phosphate
                     alpha-N-acetylglucosaminyl 1-phosphate transferase"
                     /protein_id="HCD1822975.1"
                     /translation="VTLLLVAVTMIVSLTITPIVIAISKRLNLVDKPNFRKVHTKPIS
                     VMGGTVILFSFLIGIWIGHPIETEIKPLIIGAIIMYVLGLVDDIYDLKPYIKLAGQIA
                     AALVVAFYGVTIDFISLPMGTTIHFGFLSIPITVIWIVAITNAINLIDGLDGLASGVS
                     AIGLITIGFIAILQANIFITMICCVLLGSLIGFLFYNFHPAKIFLGDSGALMIGFIIG
                     FLSLLGFKNITIIALFFPIVILAVPFIDTLFAMIRRVKKGQHIMQADKSHLHHKLLAL
                     GYTHRQTVLLIYSISILFSLSSIILYVSPPLGVVLMFVLIIFSIELIVEFTGLIDNNY
                     RPILNLISRKSSHKED"
     gene            complement(1219..1860)
                     /locus_tag="NBI57_002044"
     CDS             complement(1219..1860)
                     /locus_tag="NBI57_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HCD1822976.1"
                     /translation="MTQNIITVKKEHIIENVISKSRFIAHIKPVQNEDEAKAFIAAIK
                     KEHKDATHNCSAYTIGPEMNIQKANDDGEPTGTAGVPMLDILKKLDVHNACVVVTRYF
                     GGIKLGGGGLIRAYSGAVRDVIYDVGRVELREAVPCIVTLNYDQTGKFEYELASTHFM
                     LRNQTYTDKVSYYIDVVKSDYEAFINFLNQMTAGNFDLTEETPKQLPFDIPTE"
     gene            2004..2870
                     /gene="fakB1"
                     /locus_tag="NBI57_002045"
     CDS             2004..2870
                     /gene="fakB1"
                     /locus_tag="NBI57_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000686350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid kinase binding subunit FakB1"
                     /protein_id="HCD1822977.1"
                     /translation="MKIAVMTDSTSYLSQDLIDKYNIQIAPLSVTFDDGKNFTESNEI
                     AIEEFYNKMASSQTIPTTSQPAIGEWITKYEMLRDQGYTDIIVICLSSGISGSYQSSY
                     QAGEMVEGVNVHAFDSKLAAMIEGCYVLRAIEMVEEGYEPQQIIDDLTNMREHTGAYL
                     IVDDLKNLQKSGRITGAQAWVGTLLKMKPVLKFEDGKIIPEEKVRTKKRAIQTLEKKV
                     LDIVKDFEEVTLFVINGDHFEDGQALYKKLQEDCPSGYQVAYSEFGPVVAAHLGSGGL
                     GLGYVGRKIRLT"
     gene            3234..4316
                     /locus_tag="NBI57_002046"
     CDS             3234..4316
                     /locus_tag="NBI57_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011382223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HCD1822978.1"
                     /translation="MDNVTRYKITESSQSSSQAYYHLSFELSEQQSYASEHIVRAIRM
                     RQTILLYAVTGAGKTEMMFQGIQYARRQGDNIAIVSPRVDVVVEISKRIKDAFLNEDI
                     DILHQQSSQQFEGHFVVCTVHQLYRFKQHFDTIFIDEVDAFPLSMDKSLQQALKSSSK
                     VEHATIYMTATPPKQLLSEIPHENIIKLPARFHKKSLPVPKYRYFKLNNNKIQKMLYR
                     ILQDQINNQRYTLVFFNNIETMIKTFSVYKQKITKLTYVHSEDVFRFEKVEQLRNGHF
                     DVIFTTTILERGFTMANLDVVVIDAHQYTQEALIQIAGRVGRKLECPAGKVLFFHEGV
                     SMNMIQAKKEIQRMNKLALKRGWIDE"
     gene            4309..4983
                     /locus_tag="NBI57_002047"
     CDS             4309..4983
                     /locus_tag="NBI57_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HCD1822979.1"
                     /translation="MNNCLSCGAKLYENITIYNLFKKPNRLCDRCKENWDNIKLDIKA
                     RRCSRCLKHLNQDEAYCLDCKFLSAHFNLMEQLYCQFQYDGLMKEMIHQYKFLKDYYL
                     CELLAHLIEIPQTSYDYIVPIPSSPAHDLSRTFNPVEAVLKAKGIRFDKILKMSNRPK
                     QSHLTKKERLADENPFIIDTELDLNGKEILLVDDIYTTGLTIHRAGCKLYAKNIRKFK
                     VFAFAR"
     gene            5044..5616
                     /gene="raiA"
                     /locus_tag="NBI57_002048"
     CDS             5044..5616
                     /gene="raiA"
                     /locus_tag="NBI57_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HCD1822980.1"
                     /translation="MIRFEIHGDNLTITDAIRNYIEEKIGKLERYFNDVPNAVAHVKV
                     KTYSNSATKIEVTIPLKNVTLRAEERNDDLYAGIDLINNKLERQVRKYKTRINRKSRD
                     RGDQEVFVAELQEMQETQVDNDAYDDNEIEIIRSKEFSLKPMDSEEAVLQMNLLGHDF
                     FVFTDRETDGTSIVYRRKDGKYGLIQTSEQ"
BASE COUNT         2009 a          827 c         1002 g         1895 t
ORIGIN      
        1 ggttacatta ttactagttg cagtaacaat gattgtcagt ttgacgataa caccaattgt
       61 tattgcaata tcgaaaagat taaatttagt tgataaacca aattttagaa aagtacacac
      121 taaacctatt tcagttatgg gtggtacagt gattctcttt tcatttttaa taggtatttg
      181 gattggtcat cctattgaaa cagaaatcaa accacttatt attggtgcga ttattatgta
      241 cgtacttggg cttgtagatg atatctacga tttgaaaccg tatataaaat tggctggtca
      301 aattgccgct gccttagtag ttgcttttta tggtgtgact attgatttta tttcgttgcc
      361 aatgggtaca acgattcatt ttggatttct tagtattcca attactgtga tttggattgt
      421 tgctattaca aatgcaatta acttaattga tggactcgat ggtttggcgt cgggtgtttc
      481 tgcaatcgga ctcattacaa taggattcat tgcaatttta caagctaata ttttcataac
      541 gatgatttgt tgtgttttat taggctcttt aattgggttt ttattttaca atttccatcc
      601 tgccaaaata tttttaggtg atagtggggc tttaatgatt ggatttatca tcggattcct
      661 ttctttactc ggattcaaaa atattacaat tattgcattg ttcttcccaa ttgttatctt
      721 agcagttcca ttcattgata ctttgttcgc aatgattcga cgtgtgaaaa aagggcagca
      781 tataatgcaa gctgataaat cgcatttgca tcataaacta ttagctttag gctacacaca
      841 tagacaaaca gtattattaa tctattcaat ctctatttta tttagtcttt cgagcattat
      901 tttgtatgta tcgccaccat taggtgttgt attaatgttt gtattaatca tatttagtat
      961 tgaattaatt gttgaattta caggattaat agataacaac taccgaccaa tattaaattt
     1021 aattagtcgg aaatcgtcac acaaagaaga ttagatttta aaacagtaag taataaaata
     1081 aaaaaatgat gtctgtactt ttatgttgtt ataaaggaag acatactcac aaaaaacact
     1141 agcatggaac gtgctttcca aattgcaaag taacaatacg caattagagt tagccctcct
     1201 aagctagtgt ttttattttt attcagttgg tatatcgaaa ggtaactgct ttggagtttc
     1261 ttcagtcaaa tcgaaatttc cagcagtcat ttgatttaaa aagttaataa acgcttcata
     1321 gtcactttta acgacatcga tatagtagct taccttatca gtgtaagttt ggtttcttaa
     1381 cataaaatga gttgaagcta attcatattc aaatttacca gtttgatcat aattcagtgt
     1441 tactatacat ggtactgctt ctcgtagttc gacacgcccg acatcataaa tgacgtctct
     1501 aacagcaccg ctataggcgc gaattaaacc gccaccacct aatttaatac caccaaaata
     1561 tcttgttact acgacacacg cattatgaac atcgagcttt tttaatatgt ctaacattgg
     1621 gacaccggca gttcctgtcg gttcaccatc atcattcgct ttttgaatat tcatttcagg
     1681 tccaatagta tatgcagaac aattatgagt ggcatcttta tgttcttttt ttattgcagc
     1741 aataaatgct ttagcttcat cttcattttg aacaggtttg atatgagcaa tgaatcttga
     1801 tttactaatc acattttcaa taatgtgttc ttttttaaca gtaatgatat tttgtgtcat
     1861 aataactcct taattcataa gcttaagatt atttaatctt cattatacac tgaaaatgac
     1921 atgactataa atcgtttgat tgccattttc tttttaactg aaatattgta tcattgctat
     1981 gagtatattt taggaggacg actatgaaaa ttgctgtgat gaccgattct acaagttatc
     2041 tgtcgcagga cttaatcgat aaatataata ttcaaatagc gccattaagt gtgacttttg
     2101 atgatggaaa gaactttaca gaaagtaatg aaatagcaat tgaagaattt tataataaaa
     2161 tggcatcgtc tcaaacgatt ccgacaacaa gccaaccagc aattggcgaa tggattacta
     2221 aatatgaaat gctaagagat cagggttaca cagatatcat tgtcatttgc ttatccagtg
     2281 ggattagtgg aagttatcaa tctagttatc aagcagggga aatggttgaa ggtgttaatg
     2341 tacatgcatt tgatagtaag cttgcagcaa tgattgaagg atgttatgta ttacgtgcta
     2401 ttgaaatggt tgaagaagga tacgaaccac agcaaattat tgatgattta actaatatgc
     2461 gtgagcacac aggtgcatat ttaattgttg atgacttaaa gaatttacaa aaaagtggtc
     2521 gaattactgg tgctcaagca tgggttggaa cattattgaa aatgaagcca gttcttaagt
     2581 ttgaagatgg caagattata ccagaagaaa aagttcgtac taaaaagcgt gccattcaaa
     2641 cattagaaaa gaaagtatta gatattgtaa aagactttga agaagtaact ttatttgtca
     2701 taaatggaga tcattttgaa gatggtcaag cgttatacaa aaagttacaa gaagattgtc
     2761 cttcaggtta tcaagtagca tactctgagt ttggtccagt tgttgcagca catttaggtt
     2821 ctggtggatt aggtttaggc tatgttggca gaaaaataag attaacataa ttataaaatt
     2881 ttaataaaag agtctatatt gtaattggaa attatctctc gtatacatgg ctttaaatgt
     2941 tcatcatttg aaagtcaaaa tgctaaagat ataagaaaat cattataata ttaggctctt
     3001 ttttacgttg aaatgaggtt ttaagcatta aacattacgg gaaattaatt catcctcata
     3061 cttcacttac taatgaaaaa ataaaaaaag aagtaacagg tgtcatcaaa caaaattcaa
     3121 actattattg tgttcaatgt gaaagtacaa atccaaagca tttttatcag tatgattcct
     3181 cagtacattc caagaaaatt gtatattgca gaaattgtat atcactgggt cgaatggata
     3241 atgtaacaag atataaaata acagagagtt cgcaaagttc atcacaagca tattatcatc
     3301 tctcatttga attgtcggaa cagcagtctt atgcctcaga acatattgtt cgagccatta
     3361 gaatgagaca aacgattttg ttatatgccg taacaggtgc aggtaagaca gaaatgatgt
     3421 ttcaaggcat tcaatatgca agacgacagg gagataatat agctattgtg tcaccgcgtg
     3481 tagatgttgt tgtagaaatt agtaaacgta ttaaagacgc atttcttaat gaagatatag
     3541 acatactaca ccagcaatca agtcaacaat ttgaagggca ttttgttgta tgcacagtac
     3601 atcaacttta ccgattcaaa cagcactttg atactatttt tattgatgaa gtcgatgcct
     3661 ttcctttatc aatggataaa agtttacaac aagcattgaa gtcatcttct aaagttgaac
     3721 atgcaacgat ttatatgaca gcaacaccac cgaaacaact tctgtcagag attccccacg
     3781 aaaatataat taaattgcca gctcgctttc ataaaaaatc acttccagtt cctaaatatc
     3841 gttatttcaa acttaataat aataagattc agaaaatgtt ataccgaatt ttacaagatc
     3901 aaattaataa tcaacgttat acactggtgt tttttaacaa tatagaaaca atgattaaaa
     3961 cattttcggt ttataagcag aaaattacta aattaacata cgtccatagc gaggatgttt
     4021 ttcgctttga aaaagttgaa caattaagga atggacattt cgatgtcatt tttactacga
     4081 caatattaga acgtggattt acaatggcaa atttggatgt tgttgttatc gatgcacatc
     4141 aatatactca agaggcttta atacaaattg ctggacgtgt tggacgaaaa ttagaatgtc
     4201 ctgctggaaa agtattgttt tttcatgaag gagtaagtat gaatatgatt caagctaaaa
     4261 aagagattca aaggatgaac aaattagcat taaaaagagg ttggattgat gaataattgt
     4321 ttgagttgtg gtgctaagtt atatgaaaat ataaccattt ataatttgtt caagaaacct
     4381 aatagattat gtgacagatg caaagagaat tgggacaata ttaaacttga tattaaagca
     4441 aggcgatgtt caaggtgctt aaaacactta aatcaagatg aagcgtattg tttagactgc
     4501 aagtttctat cggcacactt taatttaatg gaacaattat attgtcaatt tcaatatgac
     4561 ggtttaatga aagagatgat acatcagtat aaatttttga aagactatta tttatgtgaa
     4621 ttattggcac atttgattga aataccacaa acatcttatg actatattgt gccaattcct
     4681 tcttcgccgg cacatgattt atctagaaca tttaacccgg tagaagcagt actaaaagct
     4741 aaagggattc gctttgataa gattttaaag atgtcaaata gaccaaaaca gtctcattta
     4801 actaagaaag agcgtctggc agatgaaaat ccatttatta ttgatacgga attagattta
     4861 aatggcaagg aaatattact cgttgacgat atttatacaa ctggattaac aattcatcgt
     4921 gcagggtgta aattatatgc taaaaatatc agaaaattca aagtgtttgc gtttgcacga
     4981 tagcgtaaaa atgttaaaat ataataaaga gttaccaata aagaggttta aggagagatt
     5041 actatgatta gatttgaaat tcatggagat aacctcacta tcacagatgc tattcgcaac
     5101 tatattgagg aaaaaattgg taagttggaa cgttatttta atgacgtacc aaatgcagtg
     5161 gcgcatgtta aagttaaaac ttattcaaat tcagctacta aaattgaagt aacaattcca
     5221 ttgaaaaatg ttacgttaag agctgaagag cgaaacgatg atttatacgc aggtattgat
     5281 ttaattaata ataaacttga aagacaagtt cgaaaatata aaacacgtat taatcgtaag
     5341 agccgtgatc gaggagatca agaagtgttt gttgccgaat tacaagaaat gcaagaaaca
     5401 caagttgata atgacgctta cgatgataac gagatagaaa ttattcgttc aaaagaattc
     5461 agcttaaaac caatggattc agaagaagcg gtattacaaa tgaatctatt aggtcatgac
     5521 ttctttgtat tcacagacag agaaactgat ggaacaagta tcgtttaccg acgtaaagac
     5581 ggtaaatatg gcttgattca aactagtgaa caataaatta agtttaaagc acttgtgttt
     5641 ttgcacaagt gcttttttat actccaaaag caaattatga ctatttcata gttcgataat
     5701 gtaatttgtt gaatgaaaca tagtgactat gct
//
LOCUS       DAHHSC010000152         5733 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.153, whole genome shotgun sequence.
ACCESSION   DAHHSC010000152 DAHHSC010000000
VERSION     DAHHSC010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 5733)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5733)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5733
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.153"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            complement(70..918)
                     /gene="dat"
                     /locus_tag="NBI57_002049"
     CDS             complement(70..918)
                     /gene="dat"
                     /locus_tag="NBI57_002049"
                     /EC_number="2.6.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino-acid transaminase"
                     /protein_id="HCD1822981.1"
                     /translation="MEKIFLNGEFVSPSEAKVSYNDRGYVFGDGIYEYIRVYNGKLFT
                     VTEHYERFLRSANEIGLDLNYSVEELIELSRKLVDMNQIETGAIYIQATRGVAERNHS
                     FPTPEVEPAIVAYTKSYDRPYDHLENGVNGVTVEDIRWLRCDIKSLNLLGNVLAKEYA
                     VKYNAVEAIQHRGETVTEGSSSNAYAIKDGVIYTHPINNYILNGITRIVIKKIAEDYN
                     IPFKEETFTVDFLKNADEVIVSSTSAEVTPVIKLDGEPINDGKVGPITRQLQEGFEKY
                     IESHSI"
     gene            complement(922..2331)
                     /gene="sapep"
                     /locus_tag="NBI57_002050"
     CDS             complement(922..2331)
                     /gene="sapep"
                     /locus_tag="NBI57_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mn(2+)-dependent dipeptidase Sapep"
                     /protein_id="HCD1822982.1"
                     /translation="MWKEKVQQYEDQIINDLKGLLAIESVRDDAKASEDAPVGPGPRK
                     ALDYMYEIAHRDGFTTHDVDHIAGRIEAGKGNDVLGILCHVDVVPAGDGWDSNPFEPV
                     VTEDAIIARGTLDDKGPTIAAYYAIKILEDMNVDWKKRIHMIIGTDEESDWKCTDRYF
                     KTEEMPTLGFAPDAEFPCIHGEKGITTFDLVQNKLTEDQDEPDYELITFKSGERYNMV
                     PDHAEARVLVKENMTDVIQDFEYFLEQNHLQGDSTVDSGILVLTVEGKAVHGMDPSIG
                     VNAGLYLLKFLASLNLDNNAQAFVAFSNRYLFNSDFGEKMGMKFHTDVMGDVTTNIGV
                     ITYDNENAGLFGINLRYPEGFEFEKAMDRFANEIQQYGFEVKLGKVQPPHYVDKNDPF
                     VQKLVTAYRNQTNDMTEPYTIGGGTYARNLDKGVAFGAMFSDSEDLMHQKNEYITKKQ
                     LFNATSIYLEAIYSLCVEE"
     gene            complement(2775..3197)
                     /locus_tag="NBI57_002051"
     CDS             complement(2775..3197)
                     /locus_tag="NBI57_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HCD1822983.1"
                     /translation="MAKGNLFKAILGIGGAVAAVLVTRKDSRDKLKAEYNKYKQDPQS
                     YKDNAKDKATQLGTIANETIKEVKTNPKEYANRLKNNPKAFFEEEKSKFTEYDNKTDE
                     SIEKGKFDDEGGAAPNNNLRIVTEEDLKKNKNALSDKE"
     gene            complement(3214..3909)
                     /locus_tag="NBI57_002052"
     CDS             complement(3214..3909)
                     /locus_tag="NBI57_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HCD1822984.1"
                     /translation="MRIDKFLANMGVGTRNEVKQLLKKGLVNVNEQVIKSPKTHIEPE
                     NDKITVRGELIEYIENVYIMLNKPKGYISATEDHHSKTVIDLIPEYQHLNIFPVGRLD
                     KDTEGLLLITNDGDFNHELMSPNKHVSKKYEVISANPITEDDIQAFKEGVTLTDGKVK
                     PAILTYIDNQTSHVTIYEGKYHQVKRMFHSIQNEVLHLRRIKIADLELDSNLDSGEYR
                     LLTENDFDKLNYK"
     gene            complement(3906..5567)
                     /locus_tag="NBI57_002053"
     CDS             complement(3906..5567)
                     /locus_tag="NBI57_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HCD1822985.1"
                     /translation="MSESKEMVRGTFLITISILITKVLGVLFIIPFNYLIGGQENMAP
                     FTYAYAPYNIAIAVATAGVPLAASKYVAKYNAIGAYKVSQKFYKSSFIVMSITGVLGF
                     LVLYFLAPYISELTLARNIHDKNGWSVDDITWIIRIISMVVIFIPVLATWRGIFQGYK
                     SMGPTAVSEVTEQIARVIFILIGSYLVLNVFDGSILLANGIATFAAAVGAIIGIFTLW
                     YYWRKRKHNIDRMVESDYTDIDVSYGKMYKEIIAYSIPFVIVSLNYPLFNLVDQFTHN
                     GALSLVGIPSQLQDIFFNMLNMSTNKIVMIPTSLSAGFAVSLIPYITKTFAEGRLHEM
                     HHQIRTSIGVLMFITVPASIGIMALAQPLFTVFYGYDPIVLGHDPNHDGSRLLFYYAP
                     VAILISLLSVTASMLQGIDKQKLTVYVILASVVIKLALNYPLIMLFHTPGAILSTSIA
                     LLFAIGCNFYILKKYAKFKFSYSWIHFAKIFLYSFIMMLGVELVFFLANLFLEPTKLG
                     YLIIIILGVTVGILIYGTITIKTRLADEFLGEIPEKLRRRVRFLR"
BASE COUNT         1868 a         1063 c          762 g         2040 t
ORIGIN      
        1 tataacataa ttcacttacg aaagttgata aatttaagta atttaatcta aaaatatgat
       61 gaaagaattt taaatactgt gtgactctat atacttttca aatccttctt gtagttgacg
      121 tgtaattggg ccaactttac catcattaat tggttcacca tctaatttaa taacaggtgt
      181 aacctcagct gaagtacttg aaacaataac ttcatctgcg tttttcaaga aatctacagt
      241 aaacgtttct tctttaaatg ggatgttata gtcttcggca atttttttaa ttacaattcg
      301 tgtaatacca ttaagaatat agttgttaat cggatgtgta taaatcacac cgtctttaat
      361 tgcataagca ttacttgaag atccttcagt tacagtttca cctcgatgtt gaattgcttc
      421 aactgcatta tatttcacag catattcttt tgctaataca tttcctaata agttcaagct
      481 tttaatgtcg caacgtaacc atcggatatc ttcaacggta acaccattca caccattttc
      541 taaatgatca taaggacgat cataactctt tgtataagca acaattgctg gttctacttc
      601 aggtgtcggg aagctatgat tcctttcagc tacaccacgc gttgcttgaa tataaattgc
      661 cccagtttca atttgattca tatcaactaa tttacgagat agttcaatta attcttctac
      721 agaataattt aaatctaaac caatctcatt ggcactacgt aaaaatcttt cataatgttc
      781 tgttactgta aataacttac cattatatac tcgaatgtat tcataaatac catcgccaaa
      841 tacgtatcct ctgtcgttgt atgaaacctt tgcttcactt ggacttacaa actcaccatt
      901 taaaaaaatt ttttccatat attattcctc cacgcataat gaataaattg cttctaagta
      961 aatactagtt gcgttaaata actgtttttt agtgatatat tcatttttct gatgcattaa
     1021 atcttcagaa tcactaaaca ttgcgccaaa tgctacaccc ttgtctaagt ttctcgcata
     1081 agtaccgcca cctatagtat aaggttcagt catatcattt gtttgatttc tatatgcagt
     1141 aactaacttt tgtacaaaag gatcattttt atcaacataa tgtggtggtt ggactttacc
     1201 taatttcact tcaaagccat attgttgaat ctcatttgca aaacgatcca tagctttttc
     1261 aaattcaaat ccttctgggt agcgtaagtt gataccgaaa agacctgcgt tttcattatc
     1321 atatgtaata acaccaatgt tagttgtcac gtcacccatg acatctgtat ggaatttcat
     1381 tcccatcttt tcaccaaaat ctgaattaaa taagtagcga ttactaaatg ctacaaacgc
     1441 ttgtgcatta ttatcaagat ttaatgatgc taagaatttt agtaagtaaa gacccgcatt
     1501 cacaccgata gatggatcca taccatgaac cgctttacct tcaactgtta aaactagaat
     1561 gccactatca acagtactat caccttgtaa atgattttgt tctaaaaagt actcaaagtc
     1621 ttgaataaca tctgtcatat tttctttaac aagcactctt gcttctgcat gatcaggtac
     1681 catgttgtaa cgttcaccag atttaaaagt tattaattca taatcaggtt catcttgatc
     1741 ttcagtaagt ttattttgaa ctaaatcaaa tgttgtaatg cctttttcac catgaataca
     1801 tggaaattct gcatctggtg caaaacctaa tgttggcatt tcttctgttt taaaatagcg
     1861 atccgtacat ttccaatcag attcttcatc cgtaccaata atcatatgaa tacgtttctt
     1921 ccaatccaca ttcatatctt ctaatatctt aattgcataa taagcagcaa ttgttggacc
     1981 tttgtcatca agtgtacctc tagctatgat agcatcttct gttacaaccg gctcgaacgg
     2041 attactatcc catccatcac cagcaggaac aacgtcaaca tgacataaga tacctaatac
     2101 gtcatttcct ttacctgcct caattcttcc tgcaatatga tccacatcat gtgttgtaaa
     2161 tccatctcta tgtgcaattt catacatgta gtctaatgcc ttacgaggac ctggaccaac
     2221 tggtgcgtct tctgatgctt ttgcatcatc tctcacactt tcaattgcta ataatccttt
     2281 taagtcatta atgatttgat cttcgtattg ttgaactttt tctttccaca ttcgaaatcg
     2341 acttcctttt ttctataagt taaattctat tttacatgaa aagatataaa aactacaata
     2401 agatgtcaga aaataataaa aaggaacaaa acgatgctat tgatatgaca caaatcataa
     2461 atagctgctt tgttcctttt ttaatttata tatttaaaat acacatattc aagagctcga
     2521 gatataagtc aatgtactag gcacacaatt taatattgac agtaattaac cgaacgaaaa
     2581 tgcgccccgg ggccccaaca cagagaattt cgaaaagata aagaaatacg ttttctttag
     2641 atattagtat ttcttatgaa tgagtttcac gcatgtattc ttctttctat atgcatatta
     2701 gctatgacta acgataaaga accactatat aataaatcat agtggttctt tatcatttct
     2761 gtcctactca catcttattc tttatcagat aatgcatttt tattcttttt taaatcttct
     2821 tcagtgacga tacgtaaatt attatttggt gctgcgccac cttcatcatc aaatttacct
     2881 ttttcaatac tttcgtcagt cttattgtca tattcggtaa attttgattt ttcttcttcg
     2941 aaaaatgctt ttggattatt ttttaatcta ttagcatatt ctttcggatt tgtttttact
     3001 tctttaattg tttcattagc aattgttcct aattgcgtcg ctttatcctt agcattatct
     3061 ttatagcttt gaggatcttg tttatattta ttatattctg ctttcagctt gtcacgacta
     3121 tctttacgtg taacaagtac agctgctaca gcgccaccta tacctaaaat cgctttaaat
     3181 aaattacctt ttgccatatc aatcgtctcc cttttattta taatttaatt tgtcaaaatc
     3241 attttcagtt aataaacgat attctcctga atctaaattg ctgtccaatt ctaaatcagc
     3301 aattttgata cgtcttaaat gtaatacctc attttgaatg ctatgaaaca ttcgtttaac
     3361 ttgatgatat tttccttcat aaattgttac gtgtgacgtt tgattatcaa tataagttaa
     3421 tattgccggc ttaaccttgc catcagtcag tgttacaccc tctttaaaag cttgaatgtc
     3481 gtcttcagtg ataggatttg ctgaaataac ttcatatttt ttagaaacat gtttgtttgg
     3541 actcattaat tcatgattaa aatcaccatc attcgttatc aataaaagcc cttctgtatc
     3601 tttatcaaga cgaccaaccg gaaaaatatt tagatgttgg tattcaggta ttaaatcaat
     3661 aacggttttt gaatgatgat cttcagttgc tgatatataa ccttttggct tatttaacat
     3721 aatatagaca ttttcaatgt attctattaa ttctccacga actgttatct tatcgttttc
     3781 tggttctata tgtgtttttg gtgatttaat tacttgttcg ttgacattta caaggccttt
     3841 tttaagtaac tgtttgacct cattgcgtgt accgacgccc atatttgcta aaaatttatc
     3901 tattctcatc gtaaaaacct aactctacgt cttaattttt caggaatttc acctaagaat
     3961 tcgtccgcaa gacgcgtttt aattgtgatt gtaccgtaaa ttagaatacc tactgtaaca
     4021 cctaaaataa taatgattaa gtaaccaagt ttagtaggtt ctaagaatag atttgcaagg
     4081 aaaaatacta attctacacc tagcatcata ataaatgaat acaagaatat ttttgcaaaa
     4141 tgaatccaac tatagctgaa tttaaacttc gcatattttt taagaatata gaaattacat
     4201 ccaattgcaa ataataatgc gatactagta cttaaaattg caccaggtgt atggaataac
     4261 ataattaatg gatagtttaa cgctaacttg ataactacag aagctaaaat aacataaact
     4321 gttaatttct gtttatctat accttgtaac attgatgccg ttacacttaa tagtgaaatt
     4381 agtattgcta caggcgcata atagaataat aagcgactac catcatggtt agggtcatga
     4441 cctaaaacaa ttggatcgta accatagaaa actgtgaata atggttgtgc caaggccata
     4501 attccaatac tagctggaac agttataaac attaatacac caatagatgt tctaatttga
     4561 tgatgcattt catgtaagcg accttctgca aatgtttttg taatataagg aattaaactc
     4621 actgcaaaac cagcacttaa tgatgtcgga atcattacaa ttttattagt tgacatattt
     4681 agcatattaa agaatatatc ttgtaactgt gaaggtatac caactaaaga taaagcaccg
     4741 ttatgtgtaa attgatctac taagttaaat aatggataat tcaaacttac aataacgaac
     4801 ggtatactat aagcaataat ttctttatac atcttgccat atgacacatc tatatctgtg
     4861 taatcagatt cgaccatacg atcaatatta tgcttacgct ttctccagta ataccagagt
     4921 gtgaatatac caataatcgc accaactgct gctgcaaaag tagcaatacc attggctaat
     4981 aaaatagagc catcaaagac atttagtact aaataacttc cgattaatat gaaaatcacg
     5041 cgtgcaattt gctcagttac ttctgacact gctgttggcc ccatagattt ataaccttgg
     5101 aatatccctc tccatgtcgc taatacagga ataaagataa caaccatact aatgattctt
     5161 ataatccaag taatatcatc gactgaccaa ccgtttttat catgaatgtt tctagctaat
     5221 gttaattcag aaatataagg tgctaagaaa tacagtacca agaaacctaa aacaccggta
     5281 atactcatta caataaaact cgatttataa aatttctgac ttactttata tgccccaata
     5341 gcattatatt tcgcaacata tttcgaagct gctaatggta cacctgctgt cgcaactgca
     5401 attgcaatat tatatggtgc ataagcgtat gtgaacggcg ccatattttc ttgtccacca
     5461 attaaatagt tgaatggaat gataaaaagt acgcccaata ccttggtaat taatatacta
     5521 atggtaatta aaaaggttcc acgcaccatt tctttacttt cactcattac gaatctccct
     5581 atctcatgtt tattaaagtt ttgtaaacta aaagctattt ctctgtaaaa tcatttttca
     5641 ttattatgaa tatatcacaa aactttattt cattgtcgta tattcaatga attatcataa
     5701 caaaattatc aacgcattgt cattgaatac tcg
//
LOCUS       DAHHSC010000153         5725 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.154, whole genome shotgun sequence.
ACCESSION   DAHHSC010000153 DAHHSC010000000
VERSION     DAHHSC010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 5725)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5725)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5725
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.154"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            <1..229
                     /locus_tag="NBI57_002054"
     CDS             <1..229
                     /locus_tag="NBI57_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein"
                     /protein_id="HCD1822986.1"
                     /translation="IILDEVTNALDKKTVNEIYEFLNDFIQSHQTKTIINITHNLSDL
                     SALPGKYFIFKDLQIEEYQSKEEVINDYINL"
     gene            235..966
                     /locus_tag="NBI57_002055"
     CDS             235..966
                     /locus_tag="NBI57_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease"
                     /protein_id="HCD1822987.1"
                     /translation="MSTLKLEFKKSISNKIIYTLGVLFIFLFLLGYFLPIGIDKVKNL
                     SYGQFFFSSYTVATEFGFLLFSFVIAYFINKEYSNKNILFYKLIGDNIFTFFYKKVAV
                     LFIECLIYIILGITIISIIYSDFSHYLLLIILFSLVILQYILVVGTISMVSPNILISL
                     GISIVYWIGSVILVAINKNIFGIVAPFEASNTMYRAVEKILNNESTFICPTEIINTVS
                     FFVLLFIVNTIVLLLSRKRWLKIGM"
     gene            complement(1128..1748)
                     /gene="srtA"
                     /locus_tag="NBI57_002056"
     CDS             complement(1128..1748)
                     /gene="srtA"
                     /locus_tag="NBI57_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase SrtA"
                     /protein_id="HCD1822988.1"
                     /translation="MKKWTNRLMTIAGVVLILVAAYLFAKPHIDNYLHDKDKDEKIEQ
                     YDKNVKEQASKDNKQQAKPQIPKDKSKVAGYIEIPDADIKEPVYPGPATPEQLNRGVS
                     FAEENESLDDQNISIAGHTFIDRPNYQFTNLKAAKKGSMVYFKVGNETRKYKMTSIRD
                     VKPTDVEVLDEQKGKDKQLTLITCDDYNEKTGVWEKRKIFVATEVK"
     gene            1942..2433
                     /locus_tag="NBI57_002057"
     CDS             1942..2433
                     /locus_tag="NBI57_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCD1822989.1"
                     /translation="MIRCAKKEDLNAILAIYNDAIINTTAVYTYKPQTIDERIAWFET
                     KQRNHEPIFVFEENGSVLGFATFGSFRPWPAYQYTIEHSIYVDASARGKGIASQLLQR
                     LIVEAKAKGYRTLVAGIDASNEASIKLHQKFNFKHAGTLTNVGYKFDYWLDLAFYELD
                     LKD"
     gene            complement(2577..2747)
                     /locus_tag="NBI57_002058"
     CDS             complement(2577..2747)
                     /locus_tag="NBI57_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1822990.1"
                     /translation="MLRLSIIFIAFIINTTITYGYTTEGTWVNLLFKSLSLSMIIVFM
                     FYYIRFVIEKKR"
     gene            complement(2809..2907)
                     /locus_tag="NBI57_002059"
     CDS             complement(2809..2907)
                     /locus_tag="NBI57_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1822991.1"
                     /translation="MIKKKHLSGSLVPDRCFLIIFHKVEKFMGNTC"
     gene            complement(2926..3825)
                     /gene="sdaAA"
                     /locus_tag="NBI57_002060"
     CDS             complement(2926..3825)
                     /gene="sdaAA"
                     /locus_tag="NBI57_002060"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HCD1822992.1"
                     /translation="MFDSIRETIDYAVENNMSFADIMVKEEMELSGKSRDEVRAQMKQ
                     NLDVMRDAVIKGTTGDGVESVTGYTGHDAAKLRDYNETHHALSGYEMIDAVKGAIATN
                     EVNAAMGIICATPTAGSSGTIPGALFKLEKTHDLTEEQMIDFLFTSALFGRVVANNAS
                     VAGATGGCQAEVGSASAMAAAAAVAIFGGSPEASGHAMALAISNLLGLVCDPVAGLVE
                     IPCVMRNAIGSGNALISADLALAGIESRIPVDEVIEAMDKVGRNLPASLRETGLGGLA
                     GTPTGEAIKRKIFGTAEDMVKNN"
     gene            complement(3839..4519)
                     /gene="sdaAB"
                     /locus_tag="NBI57_002061"
     CDS             complement(3839..4519)
                     /gene="sdaAB"
                     /locus_tag="NBI57_002061"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent
                     subunit beta"
                     /protein_id="HCD1822993.1"
                     /translation="MAKSYDYQSAFDIIGPVMMGPSSSHTAGAVKIGNSARAILGDIP
                     KNIEIHYYESFAQTHQGHGTDVAIVGGAMGYSTFDNRIKSSLEIAADDGIKIDIIEEE
                     GDSIGQHPNCAYIKSSRNDGRYIEIIGISIGGGTIKIKGIHINGLEVDLNHGLPILVV
                     DGNMTKAQVNHFINDINDMKLDCKDEFIKIDEDKCLVVIPLNKAISESTLNTIKEKYS
                     DVNVSYIN"
     gene            complement(4522..5565)
                     /locus_tag="NBI57_002062"
     CDS             complement(4522..5565)
                     /locus_tag="NBI57_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001551877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HCD1822994.1"
                     /translation="MDILLGVGTLVLVLVIMTLFLKYAPYGKQGLQALSGAACATFLP
                     QAFLSYAIGGVFDIKFLQDIGDLAGSLSGIAVGILTCLNLGVAPVFAVIVGLVLHDFK
                     LLPAFIAAYCVAFLIKWIEKKVPEGLDLIVVILFAPAIAFGLASIITPGVLATLKQIG
                     SAVTAVGDNNPYALAVILGLVIPVTGMTPLSSMVLTSLLGLTGVPMAIGALTCTGSSF
                     VNLILFRKLNIGGPSKAFAVCIEPLTQIDLIAQYPVQLYGTNALIGVVNACIVTFSGL
                     VIGVKGMATPIAGAIVLFGFNNAVTSIVTIATVIIVSIVLAYIIGTLINKFNLMNINF
                     KMPSKKNHIKESV"
BASE COUNT         1796 a         1073 c          842 g         2014 t
ORIGIN      
        1 aatcatatta gatgaagtta caaacgcttt agacaagaaa acagttaatg aaatttatga
       61 atttttaaat gattttattc aaagccatca aactaaaact attatcaata ttacacataa
      121 tttatccgat ttaagtgctt tgccgggtaa atactttatt tttaaagacc ttcaaataga
      181 agagtaccaa tcaaaagaag aagtcataaa tgattacatt aatttataat tgaggtgtcc
      241 actttgaaat tagaatttaa aaaaagtatt tccaataaaa ttatttacac gcttggtgtg
      301 ctattcatat tccttttctt acttgggtat tttttaccaa taggaataga caaagttaaa
      361 aatttaagct atggtcagtt tttctttagt tcttatacag ttgccactga atttggcttt
      421 ttattatttt catttgttat tgcatatttt attaataaag agtattcgaa taaaaatata
      481 ttattttaca agctgattgg cgataatata tttaccttct tctataaaaa agtagcagtt
      541 ctattcattg aatgtttaat ttatattatt ttaggcatca caattatttc aataatatat
      601 tcggatttct cacattactt actattaata atcttatttt cactcgttat cttacaatat
      661 atcttagttg ttggaacgat tagtatggtt tcacctaaca ttttaatcag cttaggtatc
      721 agcattgtat attggattgg ttcagtcatc ttagttgcca ttaataaaaa tatatttggt
      781 atcgttgctc cgtttgaggc gagcaacacg atgtatcgtg ccgtagaaaa aatattgaat
      841 aacgagtcaa catttatatg tcctactgaa attataaata cagtatcatt cttcgttctt
      901 ctatttatag ttaatactat tgtcttatta ctaagtagaa agagatggtt gaaaatcggt
      961 atgtagtaca taaaaaagag cggcatccat tgtcgctcct ttttattatg tatttttaca
     1021 atctccacca atgacacgtc gtcattggtg cctcacacca aacaaaaaaa cagtctcgca
     1081 atcaagactg ttttccactc aatatattca tccattagcg taatggatta tttgacttct
     1141 gtagctacaa agattttacg tttttcccaa acgcctgtct tttcattgta atcatcacaa
     1201 gtaattaatg ttaattgttt atctttacct ttttgttcat ctagaacttc tacatctgtt
     1261 ggcttaacat ctcttatact tgtcatttta tacttacgtg tttcattacc aactttaaag
     1321 tacaccatac tacctttttt ggctgcttta agatttgtaa attgatagtt cggacggtca
     1381 atgaaagtgt gtcctgcaat tgaaatattt tgatcatcta gtgattcatt ttcttctgca
     1441 aagcttacac ctctatttaa ttgttcaggt gttgctggtc ctggatatac tggttcttta
     1501 atatcagcat ctggaatttc aatatagcct gccacttttg atttatcttt cggaatttga
     1561 ggtttagctt gctgcttatt gtctttactc gcctgttctt ttacattttt atcatattgt
     1621 tcaatctttt catctttatc tttatcgtga agataattat cgatatgtgg tttagcaaac
     1681 aaatatgctg ccactaggat aagtactaca ccagcgattg tcattaatcg atttgtccat
     1741 tttttcatac gttaaggctc cttttataca tttcacaatg cctattaaca tttcatatag
     1801 tataccatta attataaaat gactcatagc aattcatttt atattataaa atttgcacat
     1861 atattgtcac gttagatttc attacacata tttgcattca aataacgaaa cgtttttaat
     1921 aattactaag ggggaattat tatgattcga tgtgctaaaa aagaagattt aaacgctata
     1981 ttagcgatat acaacgatgc cattattaat acaacagctg tttatactta taaaccgcaa
     2041 accatagacg aacgtatcgc atggtttgaa acgaaacaac gtaatcatga gcctatcttt
     2101 gtatttgagg aaaatggaag tgtattaggg tttgcgacgt tcggttcgtt tagaccttgg
     2161 ccagcatatc aatatactat cgagcattct atttatgtcg atgcttcagc tagaggaaaa
     2221 ggtatcgcta gtcaattatt acagcgttta attgtagagg caaaagctaa aggttatcgt
     2281 acacttgttg ctggtattga tgcttctaat gaagcgagta ttaaattaca tcaaaaattc
     2341 aatttcaagc atgccggcac actgaccaat gtaggctata aatttgatta ctggttagat
     2401 ttagcttttt atgaattaga tttaaaagac tagtagtgtt taaatcacat aatataaaca
     2461 agacaaccat gttaattcac ttaacataac atgctaacat ataaaactat aaacctctta
     2521 gggacaattt acatcgtccc ttttttcatt tcaaatacta tccccatagc tttgatttaa
     2581 cgctttttct caataacaaa acgaatatag tagaacatga aaacgataat catgctgagc
     2641 gataaagatt taaataatag attgacccac gttccctcag tcgtatatcc atatgtaatc
     2701 gttgtgttaa tgatgaatgc tataaagatg attgatagtc ttagcatatc atcactcctt
     2761 ttaagttatt ttagatatac gggggcgctt ttgcaatcac tattttgatt agcatgtatt
     2821 tcccataaat ttttcaactt tatgaaagat aattaagaag catctatctg gtactaatga
     2881 tccagacaga tgcttctttt ttatcaatat cttattgtta tctcattaat tatttttaac
     2941 catatcttca gctgtgccaa agattttacg tttaattgct tcgccagttg gtgtgcctgc
     3001 tagtccaccc aatccagttt cacgtaatga tgcaggaagg ttacgaccaa ccttatccat
     3061 tgcttcaata acttcatcaa caggtattct actttcaata cctgctaatg ctaaatctgc
     3121 tgaaattaaa gcgttacccg aaccaattgc atttctcata acacaaggaa tttcaacaag
     3181 tccggctact gggtcacaaa ctaaacctaa taaattactt atcgctaatg ccatagcgtg
     3241 cccggatgct tctggtgatc ctccgaatat agctactgct gcagctgcgg ccattgcaga
     3301 tgctgaacca acttcagctt ggcagccacc tgttgcacca gctacacttg cattgtttgc
     3361 tacgacacgc ccaaacaatg ctgaagtgaa taagaaatca atcatttgct cttctgttaa
     3421 atcatgtgtt ttttctaatt taaaaagtgc accgggaatg gtacccgagg aaccagctgt
     3481 tggcgttgca caaataatac ccatcgcagc attgacttca tttgttgcaa tggcaccttt
     3541 gactgcatca atcatttcat atccagacaa agcatgatgt gtttcattat aatcacgtag
     3601 tttagcagcg tcatgaccag tgtagcccgt tacactttca acgccatcac ctgtcgtccc
     3661 tttgattact gcgtctcgca tgacatctaa attttgtttc atttgcgctc gcacttcatc
     3721 acgtgattta ccgcttaatt ccatttcttc tttaaccatg atatccgcaa atgacatatt
     3781 attttctacg gcataatcta tagtctctct aattgaatca aacatgttta ttccccctct
     3841 aatttatata ggaaacgttt acgtcactgt atttctcttt aattgtattt aatgtcgatt
     3901 ctgagattgc tttatttaat ggtattacaa ccaagcattt atcttcatct atcttaataa
     3961 attcatcttt acagtctaat ttcatatcgt tgatatcatt aatgaaatga tttacttgtg
     4021 ctttagtcat atttccgtca acaactaaaa ttggtaatcc atgatttaaa tctacttcta
     4081 gtccatttat atgaatacct ttaattttaa ttgtaccacc accgattgaa ataccgataa
     4141 tttcaatata gcgaccatca ttacgagatg atttaatata agcacagttt ggatgttgac
     4201 caatactatc gccttcttct tcgatgatat ctattttaat accatcatca gctgcaattt
     4261 ctaatgaaga tttaattcgg ttatcaaatg ttgaatatcc cattgctcca cccacaatag
     4321 cgacatctgt accatgtcct tggtgtgttt gagcaaatga ttcataataa tgtatttcaa
     4381 tatttttagg aatatctccc aatattgcgc gtgctgaatt cccaatcttt actgcaccag
     4441 ccgtatgaga acttgaaggg cccatcataa ctggtccgat aatatcgaaa gcactttgat
     4501 aatcatagct ctttgccata attaaacact ctccttaata tgattctttt tgctcggcat
     4561 tttaaagttg atattcatta aattaaactt attaattagt gttccaataa tataggctaa
     4621 gacaatgctg acaataatca ctgttgcgat tgttacaatt gatgtcactg cattattaaa
     4681 gccaaacaat acgatggcac ctgcaattgg tgttgccata cctttgacac ctattactag
     4741 tccgctaaat gtcacgatac atgcgttgac gaccccaatc agtgcatttg taccatatag
     4801 ttgtactgga tattgcgcta ttaaatcaat ttgcgtcaat ggctcaatac aaactgcaaa
     4861 tgcttttgac ggtccaccaa tgtttaattt tcggaataaa ataaggttaa caaatgagct
     4921 acctgtacat gttagtgctc caatagccat aggaacacct gtcagtccta ataaacttgt
     4981 taataccatt gaacttagcg gtgtcatacc tgtaacagga atcactagtc ctaaaatgac
     5041 cgctaatgca tatggattgt tatcacctac cgcagtgaca gcactaccta tttgttttaa
     5101 tgttgctagc acaccaggtg taatgattga tgcaagtccg aaagcaattg ctggtgcaaa
     5161 taagatcacc acaattaagt ccaagccttc tggaactttc ttttcaatcc atttaattaa
     5221 aaaagctacg caataagctg cgatgaatgc tggtaataat ttaaagtcat gtaatactaa
     5281 accaacaata accgcaaata ctggtgcaac gcctaagttt aagcacgtta gaatacctac
     5341 tgcgataccg cttaaacttc ctgctaaatc cccaatatct tgtagaaatt taatatcaaa
     5401 tacgccacca atagcgtaac ttaagaatgc ttgtggtaga aatgtcgcac aagctgcacc
     5461 ggataatgct tgtagtcctt gtttaccgta cggtgcatac tttaaaaata gcgtcatgat
     5521 cactaaaacc aagactaatg tgcctacacc taacagaata tccatttccc aaaaacctct
     5581 ctctatgttt attttatttt cagaccataa acatcgtaca cccttaagaa aacgttttca
     5641 acttttatct gttatcaaat caaatattta agtgaaatat ttcatatttg tgaaagattt
     5701 ttaaaatgga ttgtttcaaa aacta
//
LOCUS       DAHHSC010000154         5715 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.155, whole genome shotgun sequence.
ACCESSION   DAHHSC010000154 DAHHSC010000000
VERSION     DAHHSC010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 5715)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5715)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5715
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.155"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            518..1477
                     /locus_tag="NBI57_002063"
     CDS             518..1477
                     /locus_tag="NBI57_002063"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HCD1822995.1"
                     /translation="MKTFGKKVVLIGDGSVGSSYAFAMVTQGVADEFVIIDIAKDKVK
                     ADVQDLNHGTVHSPSPVDVKAGEYEDCKDADLVVITAGAPQKPGETRLQLVEKNTKIM
                     KSIVKSVMDSGFDGYFLIAANPVDILTRFVKEYTGLPAERVIGSGTVLDSARLQYLIS
                     QELGVAPSSVDASIIGEHGDTELAVWSQANVAGISVYDTLKEQTGSEAKAEEIYVNTR
                     DAAYEIIQAKGSTYYGIALALMRISKAILNNENNVLNVSIQLDGQYGGHKGVYLGVPT
                     LVNQHGAVKIYEMPLSAEEQALFDKSVKILEDTFDSIKYLLED"
     gene            1755..2459
                     /gene="budA"
                     /locus_tag="NBI57_002064"
     CDS             1755..2459
                     /gene="budA"
                     /locus_tag="NBI57_002064"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HCD1822996.1"
                     /translation="MTNVLYQHGTLGTLMAGLLEGTATINELLEHGNLGIATLTGSDG
                     EVIFLDGKAYHANEHKEFIELKGDEKVPYASITNFKASKTFPLQQLSQDDVFAQIKNE
                     MLSENLFSAVKIYGTFKHMHVRMMPAQQPPYTRLIDSARRQPEEKRQDIRGAIVGFFT
                     PELFHGVGSAGFHIHFADDERAYGGHVLDFEVDDVVVEIQNFETFQQHFPVNNETFVK
                     AKIDYKDVAEEIREAE"
     gene            complement(2545..3405)
                     /locus_tag="NBI57_002065"
     CDS             complement(2545..3405)
                     /locus_tag="NBI57_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HCD1822997.1"
                     /translation="MLSVAIIGPGAVGTTIAYELQQSLPHTTLIGRHAKTITYYTVPH
                     APAQDIVVKGYEDVTNTFDVIIIAVKTHQLDAVIPHLTYLAHEDTLIILAQNGYGQLE
                     HIPFKNVCQAVVYISGQKKGDVVTHFRDYQLRIQDNALTRQFRDLVQDSQIDIVLEAN
                     IQQAIWYKLLVNLGINSITALGRQTVAIMHNPEIRILCRQLLLDGCRVAQAEGLNFSE
                     QTVDTIMTIYQGYPDEMGTSMYYDIVHQQPLEVEAIQGFIYRRAREHNLDTPYLDTIY
                     SFLRAYQQNM"
     gene            3478..4296
                     /gene="panB"
                     /locus_tag="NBI57_002066"
     CDS             3478..4296
                     /gene="panB"
                     /locus_tag="NBI57_002066"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HCD1822998.1"
                     /translation="MKTVSQLIDMKQKQTKISMVTAYDFPSAKQVEAAGIDMILVGDS
                     LGMTVLGYESTVQVTLADMIHHGRAVRRGAPNTFVVVDMPIGAVGISMTQDLNHALKL
                     YQETNANAIKAEGAHITPFIEKATAIGIPVVAHLGLTPQSVGVMGYKLQGATKEAAEQ
                     LILDAKNVEQAGAVALVLEAIPNDLAEEISKHLTIPVIGIGAGKGTDGQVLVYHDMLN
                     YGVEHKAKFVKQFADFSVGVDGLKQYDQEVKSGAFPSEEYTYKKKIMNEVNNND"
     gene            4289..5140
                     /locus_tag="NBI57_002067"
     CDS             4289..5140
                     /locus_tag="NBI57_002067"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HCD1822999.1"
                     /translation="MTKLITTVKEMQHIVKAAKRSGTTIGFIPTMGALHDGHLTMVRE
                     SVSTNDITVVSVFVNPLQFGPNEDFDAYPRQIDKDLELVSEVGADIVFHPAVEDIYPG
                     ELGIDVKVGPLADVLEGAKRPGHFDGVVTVVNKLFNIVMPDYAYFGKKDAQQLAIVEQ
                     MVKDFNHAVEIIGIDIVREADGLAKSSRNVYLTEQERQEAVHLSKSLLLAQALYQDGE
                     RQSKVIIDRVTEYLESHISGRIEEVAVYSYPQLVEQHEITGRIFISLAVKFSKARLID
                     NIIIGAE"
     gene            5142..5525
                     /locus_tag="NBI57_002068"
     CDS             5142..5525
                     /locus_tag="NBI57_002068"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015901315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HCD1823000.1"
                     /translation="MIRTMMNAKIHRARVTESNLNYVGSITIDSDILEAVDILPNEKV
                     AIVNNNNGARFETYVIAGERGSGKICLNGAASRLVEVGDVVIIMTYAQLNEEEIKNHA
                     PKVAVMNEDNVIIEMIHEKENTIVL"
BASE COUNT         1969 a          790 c         1182 g         1774 t
ORIGIN      
        1 actgattata acagatatta aatgaataag tactattttt gctaattttc taacaatttt
       61 gcacattata tttttaaaat caatttcatg tttatgtctg attggctagt ttgtatgaaa
      121 tgtaattctt tgactataaa atgatgcatc aaatatgatg agggtaaggc ggaaaatagg
      181 aggttatata gtaagtcatt taagtcgaat agccatcctt tttaaaatga aaaaaataga
      241 aagctcaata gtttgttaaa ggctttaaat agcgtcatta cgcaatttta gaatgctaaa
      301 aattgtcaca aatttcaaat tacgtgctaa agcttgagat atcaatattt attggcgata
      361 gagtgtaatt tgactccgct tacatctcag ttttatgttt gtaatttggt agcataatat
      421 tataataaaa taaaattgtt aatctttaat ttcagtatag atatttttac gtgtagtcac
      481 gtgtaaaata aattcaatta ggttaggaga cataattatg aaaacatttg gtaaaaaggt
      541 tgtattaatc ggagatggat ctgtaggatc aagctatgcc tttgcaatgg ttacgcaagg
      601 tgttgctgat gaatttgtaa ttattgacat tgcaaaagac aaagtaaaag cagatgttca
      661 agatttaaac catggtacag tccacagtcc ttcaccagtt gatgtgaaag caggtgaata
      721 cgaagactgt aaagatgcag atttagttgt tattacagct ggtgcacctc aaaagccagg
      781 tgaaacacgt ttacaattag ttgaaaaaaa tactaagatt atgaagagca ttgttaagag
      841 tgttatggat agcggctttg atggatattt cttaatcgcg gcaaaccctg ttgacatttt
      901 aacaagattt gtaaaagaat atactggttt accagcagag cgtgttatcg gttcaggtac
      961 tgtattggac agtgcacgtt tacaatattt aattagccaa gaacttggtg ttgcaccttc
     1021 aagtgttgac gctagtatta ttggtgagca tggtgatact gaacttgcag tttggtcaca
     1081 agcaaatgta gcaggtattt cagtatatga cacattaaaa gaacaaactg gtagcgaagc
     1141 taaagcggaa gaaatttatg taaatacacg tgacgctgct tatgaaatta tccaagctaa
     1201 agggtcaaca tactatggaa ttgcattagc attgatgcgc atttcaaaag ccattttaaa
     1261 taatgaaaat aatgtcttaa atgtttctat acaattagat ggtcaatatg gtggtcacaa
     1321 aggcgtttac ctaggtgtac caacattagt taaccaacat ggcgcagtta aaatttatga
     1381 aatgccatta agtgccgaag aacaagcgtt gttcgataaa tctgttaaaa tattagaaga
     1441 tacatttgat tcaattaaat atttattaga agactaagcc tattttaagt attaattaga
     1501 aatatattaa tggtaagagg atctattagt gttgcagtaa catgtggcac tgatagatcc
     1561 atttttttag caagcattga tagaggcaat acttttgcgt aaaataataa ttgaataaag
     1621 tattatgact cgtgaaaatt tagtgacatg aatcgaaatg ccgaggcgtc attgagtata
     1681 aaggaaagta gttctgcatt aagcacgaag tagagcatga caacgaggaa taactatagg
     1741 gagatggttt tggaatgacg aatgtcttgt atcaacatgg tacattaggt acgttaatgg
     1801 ctggcttact agaaggaaca gctacaatta atgaattgtt agaacatggg aatttaggga
     1861 ttgcaacgtt aacagggtct gatggcgaag taatattttt agacgggaag gcatatcatg
     1921 ctaacgagca taaagaattt atagaattaa aaggcgatga gaaagtaccg tatgcatcga
     1981 ttactaattt taaagcgagt aagacatttc cattgcaaca attatcacaa gatgatgtat
     2041 tcgcacaaat taaaaatgaa atgttaagtg agaatttatt ttcggctgtt aaaatttatg
     2101 gcacatttaa acatatgcat gtacgaatga tgcctgctca gcaaccgcca tatacacgtt
     2161 tgattgattc agcacgcaga caacctgagg aaaaaagaca agatattcgt ggtgccattg
     2221 ttggattttt tacaccagaa ttatttcatg gcgtagggtc tgctggtttt catatacatt
     2281 ttgcggatga tgaaagagct tatggtggac atgttcttga ctttgaagtg gatgacgttg
     2341 tcgttgagat acaaaacttt gaaacattcc aacaacattt cccggtaaat aacgagacgt
     2401 ttgttaaagc gaaaatagac tataaagatg tggcagaaga aattagagaa gctgaatagt
     2461 ctaatataat taaaagacct tagcgatatt aggaacaggt ggttctaaat gcatcgttaa
     2521 ggtcttttta tattatatat gtgcttacat attttgttga taagcgcgta aaaagctata
     2581 aatagtatca agatatgggg tgtcaagatt gtgttcacgt gcacgtcgat aaataaagcc
     2641 ttgaatcgct tcgacttcaa gaggttgctg atgcacaatg tcgtaataca tgctcgttcc
     2701 catttcgtcg ggataacctt gatatatagt cataatagta tcgacagttt gttctgaaaa
     2761 gtttaacccc tctgcctgtg caacacgaca accatctagt aataactgtc tacacagtat
     2821 acgtatttca ggattatgca tgattgcaac tgtctgtctc ccgagtgctg tgatagaatt
     2881 aatgcccaag ttaactaaca atttatacca aatagcttgt tgaatatttg cttctaatac
     2941 aatgtcgatt tgactgtctt ggaccaaatc tctaaattgt cgagttaatg cattatcttg
     3001 tatacgtaat tgataatctc tgaagtgcgt aacaacatcg cctttctttt gaccacttat
     3061 atagacaact gcttggcata catttttaaa tggaatatgt tcgagttgac cataaccatt
     3121 ttgggctaaa atgatgagcg tgtcctcatg tgcaagataa gttaaatgag gaataacggc
     3181 atcaagttga tgtgttttga ctgcaataat aataacatca aatgtgtttg tgacatcttc
     3241 ataacctttc acaacaatat cttgtgcagg tgcatgtggt acagtataat atgttattgt
     3301 tttggcgtgt ctcccgataa gcgttgtatg aggcaatgat tgttgtaatt cataggcaat
     3361 agttgtaccg acagcgcctg ggccgataat cgcaactgat aacatcttct tctttccttt
     3421 ctataaaaga ttcattatac tatttctaat aatactgcaa aaagaggttg aataacattg
     3481 aaaacagtaa gtcaattaat agatatgaaa caaaagcaaa ctaaaatttc tatggtaaca
     3541 gcttatgatt ttccaagtgc taaacaagtg gaagcagcgg gtattgatat gattcttgtt
     3601 ggggattcac ttggtatgac tgtattaggt tatgaaagta ccgtacaagt gacgttagca
     3661 gatatgattc atcacggtcg tgcagtaaga agaggcgcac caaatacatt tgtagttgtt
     3721 gatatgccga ttggtgcagt aggcatctct atgacacaag acttaaatca tgctctaaag
     3781 ctttatcagg agacgaatgc aaatgcaatt aaagcagaag gagcgcatat tacaccattt
     3841 attgaaaaag caacggcaat tggtattcct gttgttgcac atttaggatt aacacctcaa
     3901 agtgttggcg ttatggggta taaattacaa ggtgcaacga aagaagcagc ggagcaactt
     3961 attttagatg cgaaaaatgt agaacaagct ggtgcagttg cgttagtact tgaagcaatt
     4021 ccaaatgatt tagcagagga aattagcaag cacttaacaa ttccagtcat tggtattggt
     4081 gcaggaaaag gtacagatgg tcaagtattg gtttatcacg atatgttaaa ttatggtgtt
     4141 gaacataaag cgaaatttgt gaagcaattt gctgatttta gtgttggtgt ggatggttta
     4201 aaacaatacg atcaagaagt taaatctggt gcatttccat cagaagaata tacgtataaa
     4261 aagaaaatta tgaatgaggt taataacaat gactaagctg attactacgg taaaagagat
     4321 gcaacacatt gttaaagcag ccaaacgctc aggaacaacg attggtttta tcccaacaat
     4381 gggtgcctta catgatggac atttaacgat ggtacgcgaa tcagttagta caaatgatat
     4441 tacagttgtg agtgtatttg ttaatccatt acaatttggt ccaaacgaag atttcgatgc
     4501 ttatccgaga caaattgata aagatttaga attagtatca gaagtagggg cggacattgt
     4561 ttttcatcct gcagtagaag atatatatcc aggtgaatta ggtattgatg tcaaagtagg
     4621 tccattagct gatgtgttag aaggagcgaa gcgtccaggg cattttgatg gggtggtaac
     4681 agtagttaat aagctattta atattgttat gccggattat gcttattttg gtaaaaaaga
     4741 tgcccagcaa ttggctattg tcgagcaaat ggtaaaagac ttcaatcatg ccgttgaaat
     4801 tattggtatt gatatcgttc gagaagcaga tggtttggcg aaaagttcaa gaaatgttta
     4861 tttaacggag caagaacgac aagaagcggt acatttaagt aaaagtttgc tattagcaca
     4921 agcgttgtat caagacggtg aacgtcaaag taaagtaatt atagacaggg ttactgaata
     4981 tcttgaatca catataagtg gacgcattga agaagttgct gtttatagtt atcctcaatt
     5041 agtagaacaa cacgaaataa ctggacgcat atttatctcg ttagctgtca aattttcaaa
     5101 agcgcgttta atagataata taataattgg agctgaataa agtgataaga acaatgatga
     5161 atgctaaaat tcatagagca agagtgactg agtcaaattt aaattatgta ggtagtatta
     5221 cgattgattc agatattttg gaagcggtag acatcttgcc aaatgaaaaa gtagccatcg
     5281 tgaataataa taatggtgca cgttttgaaa catacgtaat agctggtgaa agaggtagtg
     5341 gtaaaatttg tttgaacggt gcagcatcaa gacttgttga agtaggcgat gtagtcataa
     5401 tcatgactta tgcacagtta aatgaagaag aaattaaaaa ccatgctcct aaagtagcag
     5461 taatgaatga agacaatgtc attattgaaa tgattcatga aaaagaaaat acgatagtgt
     5521 tataaaatag tataagttag aaacctctag tagataacaa tgtagttatc cattaggggt
     5581 ttttattttt atgccaattg ggttagcgta atagaagtaa aggtattatc gttgcaattt
     5641 tatcagtgtt aaatgatgga catatttata aagaaatagt tttgcgaatt gattatctaa
     5701 aacagtattc agaat
//
LOCUS       DAHHSC010000155         5629 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.156, whole genome shotgun sequence.
ACCESSION   DAHHSC010000155 DAHHSC010000000
VERSION     DAHHSC010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 5629)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5629)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5629
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.156"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            complement(22..1698)
                     /gene="kdpA"
                     /locus_tag="NBI57_002069"
     CDS             complement(22..1698)
                     /gene="kdpA"
                     /locus_tag="NBI57_002069"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit A"
                     /protein_id="HCD1823001.1"
                     /translation="MEIILFLTMMVMITYVFSGYLYRVALVQSSRVDLIFTRFENMCF
                     KIIGTDLEHMSAKTYVKHFLAFNGFMGFITFVLLIVQQWLFLNPNHILNQSIDLAFNT
                     AISFLTNSNLQHYNGESDVTYLTQMIVMTYLMFTSSASGYAVCIAMLRRLTGLTNIIG
                     NFYQDIVRFIVRVLLPLSCLISILLMTQGVPQTLHANLMIRTLSGHIQHIAFGPIASL
                     ESIKHLGTNGGGFLAGNSATPFENPNIWSNFIEMGSMMLLPMSMLFLFGRMLSRHGKR
                     VHRHALILFVAMFFIFIAILTLTMWSEYRGNPILANLGIYGPNMEGKEVRFGAGLSAL
                     FTVITTAFTTGSVNNMHDSLTPIGGLGPMVLMMLNVVFGGEGVGLMNLLIFVLLTVFI
                     CSLMVGKTPEYLNMPIGAREMKCIVLVFLIHPILILVFSALAFMIPGASESITNPSFH
                     GISQVMYEMTSAAANNGSGFEGLKDDTTFWNISTGIIMLLSRYIPIILQLMIASSLVN
                     KKSYHQDKYTIAIDKPYFGVSLIVFIVLLSGLTFIPVLLLGPIGEFLTLK"
     gene            complement(1722..1832)
                     /gene="kdpF"
                     /locus_tag="NBI57_002070"
     CDS             complement(1722..1832)
                     /gene="kdpF"
                     /locus_tag="NBI57_002070"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HCD1823002.1"
                     /translation="MLLCCKEVMAMLIVLLLVVIALVLYLFYALIRSEKF"
     gene            1970..4627
                     /locus_tag="NBI57_002071"
     CDS             1970..4627
                     /locus_tag="NBI57_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase KdpD"
                     /protein_id="HCD1823003.1"
                     /translation="MSNTESLNIGKKRGSLTIYIGYSPGVGKTFEMLSNAIELFQSNV
                     DIKIGYIEPHQRDETNALAEQLPKITTNFTKHGSHHFQYLDVDRIIEESPTIVLIDEL
                     AHTNISRDRHEKRYMDIEEILNHGIDVHTTLNIQHIESLSSQIELMTGVHVKERVPDY
                     FIMSADVLEVVDISPEQLIKRLKAGKVYKKDRLDVAFSNFFTYAHLSELRTLTLRTVA
                     DLMSDKEKVRHNHKTSLKPHIAVAISGSIYNEAVIKEAFHIAQKEHAKFTAIYIDVFE
                     KNRQYKDSQKQVHQHLMLAKSLGAKVKVVYSQTVALGLDEWCKNQDVTKLIIGQHIRN
                     KWRDFFNTPLIDHLMSFEHSYKIEIVPIKQIPVELKMNKSPYRPKGKRFAIDMLKMIL
                     IQIICVMMGLWIYQLDKHESSTIILMIFLIGIILLSIWTRSFIIGFLAAIINVFVFNY
                     FFTEPRYTFEVYRFDYPITFIVSILTSILTSALLKQIKFQYSITKKQLYRTDLLFQFN
                     DSIKQTYTVENLLINAGYQINQLLQQSITIYVINQSKVIKTIPLQNHIDNTTQQHEQA
                     LSWVIKNERQAGATTDTFPGINKWLIPIGTSPIKGILAIDYQSSQVINPYDASILESM
                     LNELSLAVENVTLLKQTRESMLQAERQLTHSNFLRSISHDIRTPLTTIMGNLDILVSH
                     SKDMSIIEKEQLLVHSFQESQYLYLLVTNILSLTKLQSSNVQIKLQPYLVSELVEEID
                     MILERRHLKKRITVSSSVNLQFIHIDSKLILQALFNLIENAVKHTSTDTKINLSIRYA
                     SYEQIEFAVIDEGPGISLEEQQKIFEPFYTGSNKYFKDNQKESMGLGLYLVQTILHKH
                     QSNLQYKPNQPHGSIFYFNIYTDFNEGDV"
     gene            4627..5322
                     /locus_tag="NBI57_002072"
     CDS             4627..5322
                     /locus_tag="NBI57_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCD1823004.1"
                     /translation="MQSKILIIEDDHAITHLLDVALTLDYYNVTTADNATQAHFKIQI
                     DKPDVILLDLGLPDKDGLCLISEIRQHTDIPIIVISARQEEQTIIQALDNGANDYMTK
                     PFNVDELRARIRVIERIAKSHQETNIVFTNGLLSIDFGSKSVVINNQEVHLTPNEFSL
                     LELLSNHKGKVLTYEMILKRIYGYVNKTEMPSLRVHMTSLRQKLSQCHEDAKDIIKTH
                     PRIGYQMLQWKEK"
BASE COUNT         2102 a          951 c          828 g         1748 t
ORIGIN      
        1 cattttttcc atccctttac tttattttaa agttaaaaat tcaccaatag gaccaagtaa
       61 tagtactgga ataaatgtca aaccacttag taaaacgata aatacgatta gtgatacgcc
      121 aaaataaggt ttatcaatcg ctattgtata tttatcttga tggtatgatt ttttattcac
      181 taaacttgat gcaatcatta attgcaaaat aattggtata taacgagaaa gcaacataat
      241 gattcctgta gagatattcc agaatgttgt atcatctttc agtccttcaa accctgatcc
      301 attgttcgca gcagctgatg tcatttcata cataacttgt gaaataccat gaaaagacgg
      361 attcgttata ctttcacttg ctccaggaat cataaaagca agtgctgaaa atactaaaat
      421 taaaattggg tgtatgagaa agactaagac aatacatttc atttcacggg cgccaattgg
      481 catatttaaa tattctggtg ttttaccaac catcaaactg catataaaca ccgtcagtaa
      541 gacaaatatc aataaattca tgagtcctac gccttcgcca ccaaatacaa catttagcat
      601 cattaatacc attggtccta atccacctat aggcgttaag ctatcatgca tgttattaac
      661 agaacccgtt gtaaatgccg tcgtaataac tgtaaatagt gctgacaaac ctgctccaaa
      721 ccgtacctct ttaccttcca tattcggtcc ataaatgcct aaattcgcta gtattggatt
      781 accacgatac tcactccaca tagttaatgt aagaattgct ataaaaatga aaaacattgc
      841 gacaaataat atcaacgcat gacgatgtac tcgtttacca tgtctactta acatgcgacc
      901 aaataagaac aacattgaca taggaagtaa catcatactg cccatttcta taaaattgct
      961 ccaaatattt ggattttcaa aaggtgttgc agaatttcct gctaaaaatc ctccaccatt
     1021 cgtaccaaga tgttttattg attcaagtga tgcaataggt ccaaatgcaa tatgttgaat
     1081 atgtccgctt aaagtccgaa tcattaaatt agcatgcaac gtttgtggta caccttgagt
     1141 catcaataaa atactaatta aacatgataa tggtaaaagt actcggacaa taaaccgaac
     1201 aatatcttga taaaaattac caatgatatt agttaatcca gttaaacgtc tcaacatcgc
     1261 tatacaaacg gcgtaacctg atgcactaga tgtaaacatt aaatatgtca ttacaatcat
     1321 ttgcgttaaa tatgtcacat ctgattcacc gttatagtgt tgtaaattac tatttgttaa
     1381 aaaagatatt gctgtattaa acgctaaatc tatcgattgg tttaaaatat gatttggatt
     1441 taaaaaaagc cattgctgaa ctattagcaa tacaaatgtt ataaacccca taaatccatt
     1501 aaatgccaga aaatgtttga catatgtttt agctgacatg tgttctaaat ctgtgccgat
     1561 aattttaaaa cacatatttt caaatctagt aaatattaaa tctactcttg acgattgcac
     1621 caatgctacg cgatatagat atccactaaa aacatacgta atcataacca tcattgttag
     1681 aaacaaaatt atttccatga taaccctcac ttaatatatt tctaaaattt ttcactacga
     1741 attaaggcat aaaataaata caaaactaat gcaataacta ccagtaataa aacgatgagc
     1801 attgccataa cctccttaca acacaacaac atcgtaacaa cttgtttatg agagaaatat
     1861 taattttcaa acttagttat taagaaatca ttaagatgtg tatgcagaaa taaattttat
     1921 agcatttaat tgtgaagaat attatgatat tgctatcgag gtgaaggtta tgtcaaacac
     1981 tgaatcgcta aacataggaa aaaagcgtgg atctttaaca atttacatcg gttacagtcc
     2041 aggtgttggt aaaacatttg agatgctttc aaatgccatt gaactatttc aaagtaacgt
     2101 tgatattaaa ataggatata ttgaaccgca tcagcgtgat gaaacaaatg cattagctga
     2161 acaattacct aaaatcacaa ccaattttac taaacatggt agtcatcatt ttcaatattt
     2221 agatgtcgac cgcataatcg aagaatcgcc gacaatagta cttatcgatg agttagcaca
     2281 tacgaatatt tctagagatc gtcatgagaa acgatatatg gatattgaag aaattttaaa
     2341 tcatggtatc gatgttcata ccactttgaa cattcaacat attgaaagtt taagtagtca
     2401 aattgaactg atgaccggtg tacatgttaa agaacgtgta cccgactatt tcataatgag
     2461 cgccgatgta ttagaagtcg tagatatctc acctgaacaa ttaattaaac gcttaaaagc
     2521 tggcaaggta tataaaaagg ataggctaga tgtagcattt agtaatttct ttacgtatgc
     2581 ccacctaagc gaactgcgta cattgacgtt aagaacagtt gccgacttga tgagtgataa
     2641 agaaaaagtc cgacacaacc ataaaacgtc actcaaacct catattgctg tggcaattag
     2701 tgggagcatt tataatgaag cagtaattaa agaggcattc catattgctc aaaaagaaca
     2761 tgcgaagttc actgctattt atatagatgt attcgaaaaa aacaggcaat ataaagatag
     2821 tcaaaagcaa gtgcatcaac atctcatgct tgcaaaatca ttaggagcaa aagtaaaagt
     2881 agtttatagc caaaccgttg cattaggatt agacgaatgg tgtaaaaatc aagatgtaac
     2941 caaattaatt atcggacaac atattagaaa taagtggcga gactttttca atacaccttt
     3001 aattgaccat ttaatgtcct ttgaacatag ctataaaatc gaaatcgttc caatcaaaca
     3061 aatacctgtt gaattgaaaa tgaacaaatc accctatcgt cctaaaggca aacgtttcgc
     3121 catagatatg ttaaaaatga ttttgattca aataatttgt gtaatgatgg gactgtggat
     3181 ttatcaactt gataagcatg agtctagtac gattatttta atgatttttc tcatcggcat
     3241 cattttatta tccatttgga cgcggtcctt catcattggc tttttagcag caattattaa
     3301 cgtatttgtt tttaattatt tttttacgga acctagatat acatttgaag tatatcgctt
     3361 tgactatcct attacattta tcgttagcat tttaacaagt attttaacga gtgctttatt
     3421 aaaacaaatt aaattccaat actccattac taaaaagcaa ctttatcgta cagatttatt
     3481 atttcaattt aatgattcga ttaaacaaac ttatacagtt gaaaacttac taataaatgc
     3541 tggataccaa attaatcaat tattgcaaca atcaattact atatacgtta tcaatcaatc
     3601 gaaagtaatt aaaacaatac cattgcaaaa ccatatcgat aatacgactc aacagcatga
     3661 acaagcatta agctgggtaa ttaaaaatga aagacaagct ggtgcaacaa cagatacatt
     3721 tccaggcatt aataaatggt tgataccaat tggcacatct ccgattaaag gcattctagc
     3781 cattgattac caaagttctc aagtgattaa tccatatgat gcatcaatat tagaatcaat
     3841 gttaaatgaa ctttctctcg ccgttgaaaa tgtgacgtta cttaaacaaa cgagagaatc
     3901 catgttacag gctgaacgtc aactgacaca ttcaaatttt ttaagatcaa tttcgcatga
     3961 catacgtaca ccgttaacaa caattatggg aaatttagat attttagtat cacatagtaa
     4021 agatatgtct atcatagaaa aggaacaatt acttgtacat agttttcaag aaagtcagta
     4081 tttatatcta ttagtgacaa atattttatc attaacaaaa ttacagtcat caaatgttca
     4141 gataaaattg caaccttatc tcgttagtga attagtagaa gaaatcgata tgattttaga
     4201 acgtcgccat ttaaaaaagc gtataactgt atcatcctcc gtaaatttac agtttataca
     4261 cattgacagt aagttgattt tacaagcttt attcaatttg attgaaaatg cagttaaaca
     4321 cacttctact gacaccaaaa tcaatttatc tattcgttat gctagctatg aacaaatcga
     4381 atttgccgtt atagacgaag gacctggaat tagtttagaa gaacaacaaa agatattcga
     4441 gcctttttac acaggatcaa ataaatattt taaagataac caaaaagaaa gcatgggact
     4501 aggcttatat ttagtacaga ctatcctaca caaacatcaa tcaaacttac aatataaacc
     4561 gaaccaacca cacggcagta tattctattt caatatatat acagacttta acgaaggaga
     4621 cgtataatgc aatctaaaat attgataatt gaagatgatc acgcaatcac acatttactt
     4681 gatgttgcat taactttaga ttattacaat gtaactacag ccgacaatgc cacacaagca
     4741 cactttaaaa ttcaaattga taaaccagat gtcattttat tagatttagg tttaccagat
     4801 aaagatggat tatgtttgat ttcagaaatc aggcaacata ctgacattcc tatcattgta
     4861 ataagtgcaa gacaagaaga acaaacaatt attcaagctt tagataacgg tgcgaatgac
     4921 tatatgacta aaccttttaa tgttgatgag cttcgggcac gaatacgagt catcgagaga
     4981 atcgctaagt cacatcaaga aactaacatt gtatttacta acggtttgct aagcatcgac
     5041 tttggctcca aatctgttgt tattaataat caggaggtac atttgacgcc aaatgaattt
     5101 agcttgttag aactattgtc aaatcataaa ggaaaagtac tcacttatga gatgatatta
     5161 aaacgaatat atggctatgt taataagact gaaatgccta gtttaagagt gcatatgaca
     5221 tcattaagac aaaaactttc acaatgtcat gaggacgcaa aagatattat taaaacacat
     5281 ccacgcattg gttatcaaat gttgcagtgg aaagagaaat aattaaataa aaaagatcgc
     5341 tgccattaaa cgaatgattg gcagtgatct tttttatatt caacttatta tttatcgcgc
     5401 actagcgtca gcaaaaagcc ctattgtttt ttataagaac atgatactga aatttcataa
     5461 agtttattta tgttcgatta ttcttaattt aagtagttgt tatattctag attcatattt
     5521 atatttacac agaatgatat aaccgcattc atatctcaat ccaattaaat atttaacctt
     5581 taaaacgtta gtactttgat atctacagag aatcaattat tgaaaacaa
//
LOCUS       DAHHSC010000156         5599 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.157, whole genome shotgun sequence.
ACCESSION   DAHHSC010000156 DAHHSC010000000
VERSION     DAHHSC010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 5599)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5599)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5599
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.157"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            112..1569
                     /locus_tag="NBI57_002073"
     CDS             112..1569
                     /locus_tag="NBI57_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500488.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HCD1823005.1"
                     /translation="MKCLIRFILVLGLLISSAMVYINPTAHAEQDQTWEKIKERGELR
                     VGLSADYAPMEFEHTVNGKTEYAGVDIDLAKKIAKDNNLKLKIVNMSFDSLLGALKTG
                     KIDIIISGMTSTPERKKQVDFSDSYMMTKNIMLVKKDKVNEYKDIKDFNNKKVGAQKG
                     TEQEKIAQTEIENASITSLSRLPDVILALKSGKVEGAVVEKPVAEAYLKQNPKLGISN
                     VKFNEEEKDTVIAVPKDSPKLLSQINKTIKEVKDKGLIDKYMTNAANAMNDDSGFISK
                     YGSFFLKGIKITILISLIGVALGSILGAFVALMKLSKIKIISWIASIYIEILRGTPML
                     VQVFIVFFGITAALGLDISALVCGTIALVINSSAYIAEIIRAGINAVDKGQMEAARSL
                     GLNYRQTMKSVIMPQAIKNILPALGNEFVTLIKESSIVSTIGVGEIMFNAQVVQGISF
                     DPFTPLLVASALYFVLTFVLTRIMNMIEGRLNASD"
     gene            1562..2284
                     /locus_tag="NBI57_002074"
     CDS             1562..2284
                     /locus_tag="NBI57_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017465289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HCD1823006.1"
                     /translation="MIKINNLNKVFGDNEVLKDINLEINQGEVVAIIGPSGSGKSTLL
                     RCMNLLEVPTKGQVIFEGNDLTEKGTQVDKLRQKMGMVFQNFNLFPHKKVVDNIILAP
                     KLLKKDNNDELHKEALSLLDKVGLKEKADVYPNQLSGGQKQRVAIARALAMHPDVILF
                     DEPTSALDPEVVGDVLKVMKDLAKEGMTMVVVTHEMGFAKDVSDKVIFMADGVVVESG
                     TPVEIFEQPQHERTQNFLARVL"
     gene            2435..3562
                     /gene="queG"
                     /locus_tag="NBI57_002075"
     CDS             2435..3562
                     /gene="queG"
                     /locus_tag="NBI57_002075"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000379983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HCD1823007.1"
                     /translation="MDTKQLKQDIIDYAYTIGIDSIGFTTADSFDELKQKLEAYHANG
                     YASGFEESDIALRTEPKLSLPTARSIIAIAVGYPNKLKGAPKSVRGDRRGLFARASWG
                     QDYHTIMRKRLDMLAAFIESKVPDVEIKSMVDTGVLSDRAVAERAGLGFVGRNGFVIN
                     PKLGTWTYLGEMLVSIPFEPDDPLLDSCGDCTICVDRCPTSALVGNGQLNSQKCISFL
                     TQTKGYMPDQYRYKIGNRLYGCDTCQQVCPKNRGINTEQDDIILEPEILKPRLVPLLR
                     MSNKEFKQTYGHLAGAWRGKKPIQRNAILALAHFNEVDAIPELKKVATTDERPMIRAT
                     AYWAIGQILGEEARDFINANYDQEDAEVQNEMIKGLDTRRE"
     gene            3567..4037
                     /gene="trmL"
                     /locus_tag="NBI57_002076"
     CDS             3567..4037
                     /gene="trmL"
                     /locus_tag="NBI57_002076"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HCD1823008.1"
                     /translation="MTNHIVLYQPEIPANTGNIARTCAGTNTHLHLIKPLGFRTDDKM
                     LKRAGLDYWEFVNITYHDSIEAFFEATNGEYYLLTKFGKKTYSDFDFSNHDKDFYFIF
                     GKETTGLPDWVKEKYQDTALRIPMSEHIRSLNLSNTAALLIYEALRQQDFPGLN"
     gene            4196..4795
                     /locus_tag="NBI57_002077"
     CDS             4196..4795
                     /locus_tag="NBI57_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate isomerase"
                     /protein_id="HCD1823009.1"
                     /translation="MAMNFKVFDNSQLVAEYAADIIRKQFNNNPTTIAGFHLDTDQAP
                     VLDELKKNVEKHAVDFSQINILDYDDKKSYFEALGVPAGQVYPIAYEKDAIELIADKI
                     KTKENKGKLTLQVVSIDEQGKLNVSIRQGLMEAREIFLVVTGANKRDVVEKLYQENGK
                     TSFEPADLKAHRMVNVILDKEAAAGLPEDVKAYFTSRFA"
     gene            4820..4972
                     /locus_tag="NBI57_002078"
     CDS             4820..4972
                     /locus_tag="NBI57_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1823010.1"
                     /translation="MSKDKDPKLNYHEEENSMVTDFEDLKELGKEMEQISDQNDQEKN
                     SEEDSQ"
BASE COUNT         2067 a          735 c         1053 g         1744 t
ORIGIN      
        1 tttaaaaaga agcttttctt tatattgata aaaatacatt tataaaacac tattgcatca
       61 aaatgtatta atatgtataa ttatgaatat taattaggga gggctgtata aatgaagtgt
      121 ttaattaggt ttatattagt attagggttg ttaattagca gtgctatggt gtatatcaat
      181 cccacagcac atgctgaaca agatcaaaca tgggagaaga ttaaagaacg cggtgaactt
      241 agagtgggtc tttctgcaga ttatgcacca atggaatttg agcatacagt taatggtaag
      301 actgagtatg caggtgtaga tattgattta gctaaaaaaa ttgcgaaaga taataattta
      361 aaattaaaaa tcgtcaatat gtcatttgat agtttgttag gagctcttaa aactggaaaa
      421 attgatatta ttatttccgg aatgacttca acgcctgaac gtaagaagca agttgatttt
      481 tcagattcat atatgatgac taaaaatatc atgcttgtaa agaaagataa agttaatgaa
      541 tataaagata tcaaagactt taataataaa aaagtagggg cacaaaaggg aactgaacaa
      601 gaaaaaatcg ctcaaaccga aattgaaaat gcatctatta cttcattaag ccgtttacca
      661 gacgttatat tagcattgaa aagtggaaag gttgaaggtg ctgtagttga aaaacctgta
      721 gcagaagcat atttaaaaca aaatcctaaa ttaggaattt ccaatgtgaa atttaatgaa
      781 gaagaaaaag atacagtgat agctgtgcca aaagattcac caaaattatt gtcacaaatt
      841 aataaaacga ttaaagaggt taaagataaa ggattaatcg ataaatatat gactaatgct
      901 gcaaatgcga tgaatgatga cagtggtttt atttctaagt atggtagttt tttcttgaaa
      961 ggaattaaga ttacaatatt aatttcactt atcggtgttg cattaggttc tattttaggt
     1021 gcattcgttg cgttaatgaa attaagtaaa ataaaaatta tttcatggat tgcttctatc
     1081 tatatcgaaa tattaagagg aacaccaatg ttagttcaag tatttatcgt attctttggt
     1141 ataactgctg cattaggact agacatttca gctcttgttt gtggaacaat tgcattagtt
     1201 attaattcct cagcttacat tgctgaaatt attcgtgcag gtataaatgc tgttgataaa
     1261 ggacaaatgg aagccgcacg tagtctaggt ttgaattata gacaaacgat gaaaagtgta
     1321 attatgccac aagcaattaa aaatatttta ccagctttag gtaatgaatt tgtcacttta
     1381 attaaagaat catctattgt gtcaacaatt ggagttggcg aaattatgtt taatgcacaa
     1441 gtggttcaag gtatttcatt tgacccattc acaccattac tagtggcatc agcattatac
     1501 tttgtcttaa catttgtact tacacgcatt atgaacatga ttgaagggag attgaatgcc
     1561 agtgattaaa ataaacaatc ttaataaagt ttttggagat aatgaagttt taaaagatat
     1621 caatcttgaa atcaatcaag gggaagtagt agcaataata ggtccatctg gtagtggtaa
     1681 aagtacattg ttaagatgta tgaatttatt agaagtaccc actaaaggtc aagtgatttt
     1741 tgaaggcaat gacttaacgg aaaaagggac acaagtagat aaactacgtc aaaaaatggg
     1801 tatggtattt caaaacttca acctatttcc acataaaaaa gttgtcgata atattatttt
     1861 agctcctaaa ttattaaaga aagataataa cgatgaatta cataaggaag cattgtcgtt
     1921 attagataaa gtgggattaa aagaaaaagc agatgtatat ccgaatcaat tatcaggtgg
     1981 tcaaaagcaa agggttgcaa ttgcaagagc tttagcaatg catccagatg ttattttatt
     2041 cgatgaacca acttcagcat tagatcctga ggtagttggt gatgtattaa aagtaatgaa
     2101 agacctagcc aaagaaggta tgaccatggt ggttgtgaca catgaaatgg gatttgccaa
     2161 agatgtaagt gacaaagtca tatttatggc agatggcgtt gtcgtagagt caggcacacc
     2221 agtcgaaata tttgaacaac cgcaacatga aagaacacaa aatttcttag caagagtatt
     2281 ataacaacct aacgaagctt gaatatatga tgcgcaccac aaagttatat catatattca
     2341 agctttttat tattaaaatt atgtgaaatg aaacctcaat atgaataatg gttgtgttaa
     2401 aataacatag tattatttta tggggggcat acgattggat acaaagcagt taaagcaaga
     2461 tatcattgac tatgcatata caattggtat cgacagtatt ggatttacaa ctgccgattc
     2521 ctttgatgaa ttgaagcaaa agctagaagc atatcatgca aatggttatg cctcaggatt
     2581 tgaagaatct gatattgctt tacgaacgga gcctaaatta tccttaccaa cagcaaggtc
     2641 aatcatagca attgcagttg gttatcctaa caaactgaaa ggtgcaccta agagtgttag
     2701 aggtgatcgc agaggcttat ttgctagagc atcgtggggt caagattatc atacaattat
     2761 gcgtaaacga ttagacatgt tagctgcatt tattgaatct aaagttccag atgttgaaat
     2821 caaatctatg gtagatacgg gtgtattatc agatagggca gtagcagaac gtgcaggttt
     2881 aggatttgtt ggtcgtaatg gctttgtcat caatcctaaa ctaggaacat ggacatacct
     2941 cggtgaaatg ttagtcagta taccttttga acctgatgat ccattattag atagctgtgg
     3001 tgattgtaca atttgtgttg atcgttgtcc aacaagtgca ttagttggaa atggccaatt
     3061 aaatagtcag aaatgcatta gttttttaac gcaaacaaaa ggctatatgc ctgaccaata
     3121 tcgttataaa attgggaata gattatatgg ttgcgatacg tgtcaacaag tttgtccgaa
     3181 aaatagaggt attaataccg aacaagatga catcattttg gaaccagaaa ttttaaagcc
     3241 aagattagta cctttactac gtatgtctaa taaagaattc aaacaaacat atggtcacct
     3301 tgcaggtgct tggcgtggta aaaagccgat acaacgaaat gctattttag cattagcgca
     3361 ttttaatgaa gtggatgcaa ttccagaatt gaaaaaagtt gcaacaacag atgaaagacc
     3421 gatgattcga gcaactgctt attgggccat tggtcaaatt cttggggaag aagcaagaga
     3481 ttttatcaat gctaattatg atcaagagga tgcagaagtt cagaatgaaa tgataaaagg
     3541 attagacaca aggagagaat agtaatatga caaatcatat cgttttatat caaccagaaa
     3601 tcccagctaa tactggtaac attgcacgta cgtgtgcagg tacaaacaca catttacatt
     3661 taattaaacc tcttggattt agaactgatg ataaaatgtt aaaaagagcc ggtttagatt
     3721 attgggaatt tgtgaatatt acgtatcatg atagtattga agcatttttt gaagccacaa
     3781 atggcgagta ttacttatta acaaaatttg gtaaaaagac ttatagtgat tttgattttt
     3841 caaatcatga caaagatttt tacttcattt ttggaaaaga gacgacagga ttaccagact
     3901 gggtgaaaga gaaatatcaa gacacagcgt taagaattcc tatgagtgaa catattcgtt
     3961 cattaaattt atcaaatact gctgcgttgt taatttatga agcgttacgt caacaagatt
     4021 ttccaggatt gaattaaaat tcgtctattt tcattataaa aatgatactt aagtgctata
     4081 tttagagtat aaccatgtta aagttaaatg gtttgatata ttgaaagtta gggtataata
     4141 attattaata tcgccgtttt tatgtaaaat gcaaactaag aggagtgtag aaatcatggc
     4201 aatgaacttt aaagtctttg acaatagtca acttgtagca gaatatgctg ctgatattat
     4261 tagaaagcaa tttaacaata atcctactac aattgcaggt tttcatttag atacagatca
     4321 agcgccagtt ctagatgaat taaagaaaaa tgttgaaaaa catgctgttg attttagcca
     4381 aataaatatt ttagattatg acgataaaaa atcatatttc gaagcgttag gtgtaccagc
     4441 aggtcaagtt tatccaattg cttatgaaaa agatgcaatc gaattaatcg ctgataagat
     4501 taaaactaaa gaaaataaag ggaaattaac attacaagtt gtttctatcg atgagcaagg
     4561 taagttaaat gttagtattc gtcaaggact aatggaagca agagaaattt tcttagtagt
     4621 gacaggtgct aataaacgag atgtagttga aaaattatat caagaaaatg gtaaaacaag
     4681 cttcgaacca gccgatttaa aagcacatag aatggtaaat gttattcttg ataaagaagc
     4741 ggctgcaggt ttacctgaag atgttaaagc ttactttacg tcacgctttg cttaattaaa
     4801 aatctgtgag gtattgatta tgtctaaaga caaagatcca aaattaaatt atcatgaaga
     4861 agaaaacagt atggtaacgg attttgaaga tttaaaagaa ttaggtaaag aaatggaaca
     4921 aatctctgat caaaatgatc aagaaaaaaa ttctgaagaa gacagtcagt aaagtattat
     4981 aattgtgtaa tattcagtta tttatcatgt aaataatgta tagtatagaa catatatcaa
     5041 ataggattga ttatcttgaa tatgaagtta ggtagcattt tgttacctag cttttttatt
     5101 atattcatag aagctgactt tctgccagct tctgagtttg tggcccgttt ccccaacttg
     5161 cactgtttgt agaaattaga aatccgattt cactatgttg gggcctcgcc aacttgcatg
     5221 aaatagtaag ctgactttcc accagcttct atgtttgtgg actgcagtta actactgcca
     5281 atataacttt gtaaagcata aaacattgat ttgtgtacca gacatagtaa tatatcaaaa
     5341 gttcaatttt aaatattaaa acaccattta aacggaattg ataattaatt actccagaca
     5401 taattgaaag aagtcatcta tcagtaatat ttttaatgaa atcattaaac cgcgtttctt
     5461 aatatgccaa gattatgata tgctattgaa gaatgaaatt agaggagatt tatatattta
     5521 tgttaggaaa atactggatt caattattga tagcaacagt tattataagt ttaatatcaa
     5581 ttaaagcttt tccattggc
//
LOCUS       DAHHSC010000157         5583 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.158, whole genome shotgun sequence.
ACCESSION   DAHHSC010000157 DAHHSC010000000
VERSION     DAHHSC010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 5583)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5583)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5583
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.158"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            complement(<1..299)
                     /locus_tag="NBI57_002079"
     CDS             complement(<1..299)
                     /locus_tag="NBI57_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002454321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1823011.1"
                     /translation="MILSILLIFFCIRLVSLKISINHSKQLKADGAVEYGVKNSKFLA
                     ITHVLIYVLAGVEAFINKDTFSFANGIGLVILIFAYIMLFMVIKTLGGIWTLKLF"
     gene            532..1911
                     /gene="uhpT"
                     /locus_tag="NBI57_002080"
     CDS             532..1911
                     /gene="uhpT"
                     /locus_tag="NBI57_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexose-6-phosphate:phosphate antiporter"
                     /protein_id="HCD1823012.1"
                     /translation="MNFFDIHKIPNKGIPLSVQRKLWLRNFMQAFFVVFFVYMAMYLI
                     RNNFKAAQPFLKEEIGLSTLELGYIGLAFSITYGLGKTLLGYFVDGRNTKRIISFLLI
                     LSAITVLIMGFVLSYFGSVMGLLIVLWGLNGVFQSVGGPASYSTISRWAPRTKRGRYL
                     GFWNTSHNIGGAIAGGVALWGANVFFHGNVIGMFIFPSVIALLIGIATLFIGKDDPEE
                     LGWNRAEEIWEEPVDKENIDSQGMTKWEIFKKYILGNPVIWILCVSNVFVYIVRIGID
                     NWAPLYVSEHLHFSKGDAVNTIFYFEIGALVASLLWGYVSDLLKGRRAIVAIGCMFMI
                     TFVVLFYTNATSVMMVNISLFALGALIFGPQLLIGVSLTGFVPKNAISVANGMTGSFA
                     YLFGDSMAKVGLAAIADPTRNGLNIFGYTLSGWTDVFIVFYVALFLGMILLGIVAFYE
                     EKKIRSLKI"
     gene            complement(2270..3028)
                     /locus_tag="NBI57_002081"
     CDS             complement(2270..3028)
                     /locus_tag="NBI57_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCD1823013.1"
                     /translation="MFKVVICDDERIIREGLKQIIPWGDYHFNTIYTAKDGVEALSLI
                     QQHQPELVITDIRMPRKNGVDLLNDIALLDCNVIILSSYDDFEYMKAGIQHHVLDYLL
                     KPVDHAQLEVILGRLVRTLLEQQSQNGRSLASCHDAFQPLLKVEYDDYYVNQIVDQIK
                     QSYQTKVTVSDLIQHIDVSESYAMRTFKDHVGITIVDYLNRYRILQSLQLLDRHYKHY
                     EIADKVGFSEYKMFSYHFKKYLQMSPSDYCKQAK"
     gene            complement(3021..4577)
                     /locus_tag="NBI57_002082"
     CDS             complement(3021..4577)
                     /locus_tag="NBI57_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HCD1823014.1"
                     /translation="MTAYKPYRHQLRRSLFASTIFPVFLVIIIGLVSFYAIYIWIEHR
                     TIHQHVDESQSSLHHTEKQIQTFITQHNNSFQELDLTNHHDVTATKRELLKLIHQQPA
                     TLYYELSGPNQFITNNYEHLNTKNMYLFSTHQLKFNNSTYMLKIYMANTPRLSEIKKD
                     SRQFALIVDQYDNILYANDDRFTIGEKYRPQQFGFMNESVKLNHADHRLIIYKDIHEN
                     IEDGITLLIVMAVVLVLLVIFGFISADNMAKRQTKDIETIIQKIYYAKNRHLGTYTPL
                     KNNSELEEINNYIYDLFESNEQLIHSIEHTERRLRDIQLKEIERQFQPHFLFNTMQTI
                     QYLITLSPKLAQTVVQQLSQMLRYSLRTNSHTVELNEELNYIEQYVAIQNIRFDDMIK
                     LHIESSEEARHQTIGKMMLQPLIENAIKHGRDTESLDITIRLTLARQNLHVLVCDNGI
                     GMSSSRLQYVRQSLNNDVFDTKHLGLNHLHNKAMIQYGSHARLHIFSKRNQGTLICYK
                     IPLSRGNVDV"
     gene            complement(4574..5542)
                     /locus_tag="NBI57_002083"
     CDS             complement(4574..5542)
                     /locus_tag="NBI57_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HCD1823015.1"
                     /translation="MKSKIYILLLFLIFLSSCANTRHSESDKNVLTVYSPYQSNLIRP
                     ILNEFEKQEHVKIEIKHGSTQVLLSNLHNEDFSERGDVFMGGVLSETIDHPEDFVPYQ
                     DTSVTQQLEDYRSNNKYVTSFLLMPTVIVVNSDLQGDIKIRGYQDLLQPILKGKIAYS
                     NPNTTTTGYQHMRAIYSMHHRVSDVHQFQNHAMQLSKTSKVIEDVAKGKYYAGLSYEQ
                     DARTWKNKGYPVSIVYPIEGTMLNVDGIALVKNAHPHPKRKKLVQYLTSRSVQQRLVA
                     EFDAKSIRKDVSEQSDQSIENLKNIPLIPKSKLPDIPHHKFLEMIQ"
BASE COUNT         1741 a          872 c         1001 g         1969 t
ORIGIN      
        1 atgaataatt ttaatgtcca aataccacct aaagttttaa taaccataaa taacatgata
       61 taagcaaaga ttaatataac taagccaata ccatttgcaa agctaaatgt atctttatta
      121 ataaatgcct ctacaccagc caatacataa attaaaacgt gtgttattgc taaaaacttc
      181 gaatttttaa cgccatattc aactgcaccg tctgctttta attgttttga gtgattaata
      241 gatatcttta agctgacaag tctgatacag aaaaagataa gtaatataga tagaatcatg
      301 atgtcctccg tcattatgtc atatgtataa gcgttgattt tgacaacata aagtatttta
      361 tagataaaac ttgtcaaata ctattaacta tttattaatt ttagtacata aatatgtttc
      421 taagtatgtg tttatgttca gtattttgga taatttaata attttaagga tattaagcgc
      481 ttacaccgac gtgatatatt tggcttaacg aaaatgattg aggtgacaga gatgaacttt
      541 tttgatatcc ataagattcc gaacaaaggc attccattat cggtacaacg taaattatgg
      601 cttagaaact tcatgcaagc tttctttgta gtgttctttg tttatatggc tatgtattta
      661 attcgaaaca actttaaggc ggcccaaccg tttttaaaag aggaaattgg attatctaca
      721 ttagaacttg gttatatcgg attagcattt agtatcacgt acggtttagg gaaaacatta
      781 cttggatatt ttgtcgatgg acgtaacaca aaacgtatta tctcgttctt acttatctta
      841 tctgcgatta cagttttaat tatgggattt gttttaagtt actttggttc tgtaatggga
      901 ttattaattg tactttgggg acttaacggg gtgttccaat cagttggtgg acctgcaagt
      961 tattcaacga tttcaagatg ggcgccaaga acgaaacgtg gccgatactt aggattttgg
     1021 aatacatcac ataatatcgg tggtgccatt gcaggtggtg ttgcactttg gggtgctaat
     1081 gtattcttcc atggaaatgt tatagggatg ttcattttcc catcggtgat tgcattactt
     1141 attggtatcg caacattatt tatcggaaaa gatgatccag aagaattagg atggaatcgt
     1201 gctgaagaaa tttgggaaga gccggtcgat aaagaaaata ttgattctca aggtatgacg
     1261 aaatgggaga tctttaaaaa atatatcctg ggaaatcctg ttatatggat tctatgtgtt
     1321 tcaaacgtct ttgtatacat tgtacgaatc ggtattgata actgggcacc gttatatgtg
     1381 tcagagcatt tacactttag taaaggcgat gcagttaata cgatattcta ctttgaaatt
     1441 ggtgcattag ttgcaagttt attatggggc tacgtatcag acttattaaa aggtcgtcgt
     1501 gcaattgtag ctattggctg tatgtttatg attacatttg ttgtcttatt ctacacaaat
     1561 gctacaagtg tcatgatggt taacatttca ttgtttgcat taggtgcgtt aatctttggt
     1621 ccgcaattat taattggtgt atcattgact ggttttgttc ctaaaaatgc catcagtgta
     1681 gcaaacggaa tgacaggttc attcgcgtat ctattcggtg actcaatggc gaaagttggt
     1741 ttggcggcta ttgctgatcc aacacgtaac ggtttaaaca tctttggata tacattaagt
     1801 ggatggacag atgttttcat cgtcttctat gttgcattat tcctaggcat gattctatta
     1861 ggaatcgttg ctttctatga agaaaagaaa attagaagtt taaaaattta atataaatcg
     1921 gattaaaagt atcgccaatc tattgcaata tagttggcga tcctgccccg acggcatgtg
     1981 cgtgaagaga tgaaagatac tgcttctacc cttgcaaata tatcatctct atgtctcggg
     2041 gcagatcata attccctgtt atgaagtatc cttatttgcc cgacttaggg tgactcaatg
     2101 aatttactcc ttacaataaa gacatatagc ggtgtcaata ttgtagggag tattgtttta
     2161 tatttaaact ctctaaaaag cggactgaaa gaaaagtgaa aacttctcta tcagtccgct
     2221 ttttcataga acaaaatgta ggcgccataa tcattagtta tgtgctaatc tatttagctt
     2281 gcttacaata atcacttggc gacatttgta aatatttttt aaaatgatag ctaaacattt
     2341 tatactctga aaagcctact ttgtctgcaa tttcatagtg tttgtaatgt cgatctaaca
     2401 attgcagaga ttgtaaaata cgatagcgat ttaaataatc gacaattgta ataccaacat
     2461 gatctttaaa tgttcgcatc gcatacgatt cactaacatc gatatgttga attaaatctg
     2521 aaacagtcac tttcgtttga taagattgct taatttgatc aacaatctga tttacataat
     2581 aatcatcgta ttctactttt aatagtggtt ggaaggcatc atgacaagat gctaagctac
     2641 ggccgttctg tgattgttgc tctaataagg tacggacaag tcttcctaaa ataacttcta
     2701 attgtgcatg gtctactggt tttaataaat aatcaagaac atgatgttga atgccggctt
     2761 tcatatattc aaagtcatcg taactcgata atatgatgac attacaatct agaagcgcaa
     2821 tatcattgag taaatcgacg ccatttttac gtggcatacg aatatcagta attactaatt
     2881 ctggctgatg ttgttgaatt agtgataatg cttcaacacc atctttagca gtgtatattg
     2941 tattgaaatg atagtctccc caaggaatga tttgctttaa accttctcga ataattcgtt
     3001 catcatcaca aataactacc ttaaacatct acattccccc ttgaaagtgg tattttataa
     3061 caaattaacg taccttgatt acgctttgaa aaaatatgaa gtcgtgcatg tgaaccatat
     3121 tgaatcattg ctttattgtg taaatgattt aatcccaaat gcttagtatc aaatacatca
     3181 ttattaagag attggcgtac atattgcagg cgagatgacg acatcccgat accattgtcg
     3241 caaactaaaa catgtaaatt ctgacgtgcc aatgtcaggc gtatagtaat gtccaatgac
     3301 tcagtatctc taccatgttt aatagcattt tctatgagtg gctgaagcat cattttacca
     3361 attgtctggt gacgcgcttc ttcagaactt tcaatatgga gcttaatcat gtcatcaaaa
     3421 cggatgtttt gtattgcaac atactgttca atgtagttca actcttcgtt taattccact
     3481 gtatgtgagt ttgtacgtaa tgagtaacgt aacatttgcg ataattgttg gaccacagtt
     3541 tgtgctaatt tcggagataa cgtaattaaa tattgtattg tttgcatcgt attgaatagg
     3601 aaatgaggct ggaattggcg ttctatttcc tttaactgaa tatcacgcaa gcgacgttct
     3661 gtatgctcga tagaatggat cagttgctca tttgattcaa ataaatcgta aatataatta
     3721 ttaatttctt ctagttcact gttgtttttt aaaggcgtat atgtacctag atgacgattt
     3781 ttggcatagt aaattttttg aataatcgtt tcgatatctt ttgtttgtcg tttagccata
     3841 ttatctgcgc taatgaaacc aaatattact agtaaaacaa gaactacggc cataacaatt
     3901 aacaacgtga taccatcttc aatgttttca tgtatatctt tataaataat gagacgatgg
     3961 tcagcatggt ttaattttac agattcattc ataaatccga attgttgcgg tctatacttt
     4021 tcacctatag taaaacggtc atcgttggcg tataaaatat tgtcatattg atcaacgata
     4081 agtgcgaatt gtcggctatc tttcttaatt tcacttaaac gtggggtgtt agccatataa
     4141 attttaagca tatatgtact attattgaat ttaagctgat gcgttgaaaa taaatacata
     4201 tttttagtgt ttaaatgttc ataattattg gttataaact gatttggtcc agataattca
     4261 taataaagtg ttgcgggctg ttggtgtatt aattttaata attcacgttt tgtagcggta
     4321 acatcatgat gatttgttaa atcgagctct tgaaacgaat tattatgctg tgtaataaat
     4381 gtctgaatct gcttttcagt gtgatgtaaa gatgactgac tttcatcaac atgttgatga
     4441 atcgtacgat gctcaatcca aatatagatg gcatagaagc ttactagtcc aataataatg
     4501 actaaaaata ctggaaaaat agtagacgca aataacgatc gtctcaattg atgtctataa
     4561 ggtttgtatg ccgtcattga atcatctcca aaaatttatg atgtggaatg tccggtaatt
     4621 tagatttcgg tattaaaggt atgttcttaa gattttcgat agactgatcg ctttgttcac
     4681 taacatcctt tcgaattgac ttggcatcga actctgcaac taatcgttgt tgtactgagc
     4741 ggcttgttaa atattgcact aactttttac gcttaggatg agggtgtgca tttttaacta
     4801 aagcaatacc atcaacattt aacattgttc cttcaattgg ataaacgatt gatacaggat
     4861 aacctttgtt tttccatgtg cgtgcatctt gttcgtagct tagacctgcg taatatttac
     4921 cttttgcaac atcttcaatg actttagacg tctttgacag ttgcatcgca tggttttgga
     4981 attgatgcac atcacttact cgatgatgca tgctataaat agcgcgcata tgttgatagc
     5041 ctgtcgttgt tgtatttgga tttgagtacg caattttacc tttaagtata ggttgtaata
     5101 aatcttgata acctcgaatc ttaatatctc cttgtaaatc tgaattcact actataactg
     5161 ttggcattaa tagaaaacta gtaacatatt tattgttcga gcgataatcc tctaattgct
     5221 gtgttacaga tgtatcttga tagggaacaa aatcttctgg atgatcaatt gtttctgata
     5281 acacaccacc cataaagacg tcaccacgct ccgaaaaatc ttcgttatgc aagtttgaaa
     5341 gcagtacttg agtagatcca tgtttgattt caattttgac atgctcttgt ttttcaaatt
     5401 catttaaaat tggacgaatc aagtttgatt gatacggaga ataaactgtt aatacatttt
     5461 tatctgattc agagtgacgc gtattagcgc atgatgataa aaaaatgaga aataatagca
     5521 agatataaat ttttgatttc atgatatccc atcaattcta tgtatatttt aatacaataa
     5581 ttt
//
LOCUS       DAHHSC010000158         5549 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.159, whole genome shotgun sequence.
ACCESSION   DAHHSC010000158 DAHHSC010000000
VERSION     DAHHSC010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 5549)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5549)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5549
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.159"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            89..973
                     /gene="ylqF"
                     /locus_tag="NBI57_002084"
     CDS             89..973
                     /gene="ylqF"
                     /locus_tag="NBI57_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HCD1823016.1"
                     /translation="MVIQWYPGHMAKAKREVSEQLKKVDVVFELVDARIPYSSRNPMI
                     DEVINQKPRVVILNKKDMSNLNEMSKWEQFFIDKGYYPVSVDAKHGKNLKKVEAAAIK
                     ATAEKFEREKAKGLKPRAIRAMIVGIPNVGKSTLINKLAKRSIAQTGNKPGVTKQQQW
                     IKVSNALQLLDTPGILWPKFEDEEVGKKLSLTGAIKDSIVHLDEVAIYGLNFLIQNDL
                     ARLKSHYNIEVPEDAEIIAWFDAIGKKRGLIRRGNEIDYEAVIELIIYDIRNAKIGNY
                     CFDIFKDMTEELANDANN"
     gene            957..1724
                     /locus_tag="NBI57_002085"
     CDS             957..1724
                     /locus_tag="NBI57_002085"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HCD1823017.1"
                     /translation="MTLTIKEVTQLINAVNTIEELENHECFLDERKGVQNAIARRRKA
                     LEKEQALKEKYVEMTYFENEILKEHPNAIICGIDEVGRGPLAGPVVACATILNSNHNY
                     LGLDDSKKVPITKRLELNEALKNEVTAFAYGIATAEEIDEFNIYKATQIAMQRAIDGL
                     SVQPTHLLIDAMTLDNALPQVSLIKGDARSVSIAAASIMAKVFRDDYMTQLSKDYPEY
                     GFEKNAGYGTKQHLLAIDDIGIMKEHRKSFEPIKSLL"
     gene            1833..2999
                     /gene="sucC"
                     /locus_tag="NBI57_002086"
     CDS             1833..2999
                     /gene="sucC"
                     /locus_tag="NBI57_002086"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006190114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HCD1823018.1"
                     /translation="MNIHEYQGKEIFRSMGVAVPEGRVAFTAEEAVEKAKELNSDVYV
                     VKAQIHAGGRGKAGGVKIAKSLSEVETYAKELLGKTLVTHQTGPEGKEIKRLYIEEGC
                     AIQKEYYVGFVIDRATDQVTLMASEEGGTEIEEVAAKTPEKIFKETIDPVIGLSPFQA
                     RRIAFNINIPKESVNKAAKFLLALYNVFIEKDCSIVEINPLVTTADGDVLALDAKINF
                     DDNALFRHKDVVELRDLEEEDPKEIEASKHDLSYIALDGDIGCMVNGAGLAMATMDTI
                     NHFGGNPANFLDAGGSATREKVTEAFKIILGDENVKGIFVNIFGGIMKCDVIAEGIVE
                     AVKEVDLTLPLVVRLEGTNVELGKKILKDSGLAIEPAATMAEGAQKIVKLVKEA"
     gene            3021..3929
                     /gene="sucD"
                     /locus_tag="NBI57_002087"
     CDS             3021..3929
                     /gene="sucD"
                     /locus_tag="NBI57_002087"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HCD1823019.1"
                     /translation="MSVFIDKNTKVMVQGITGSTALFHTKQMLDYGTKIVAGVTPGKG
                     GQVVEGVPVFNTVEEAKNETGATVSVIYVPAPFAADSILEAADADLDMVICITEHIPV
                     LDMVKVKRYLQGRKTRLVGPNCPGVITADECKIGIMPGYIHKKGHVGVVSRSGTLTYE
                     AVHQLTEEGIGQTTAVGIGGDPVNGTNFIDVLKAFNEDDETKAVVMIGEIGGTAEEEA
                     AEWIKANMTKPVVGFIGGQTAPPGKRMGHAGAIISGGKGTAEEKIKTLNSCGVKTAAT
                     PSEIGSTLIEAAKEAGIYESLLTVNK"
     gene            4156..5274
                     /locus_tag="NBI57_002088"
     CDS             4156..5274
                     /locus_tag="NBI57_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HCD1823020.1"
                     /translation="MNKQQSKVRYSIRKVSIGILSISIGMFLALGMSNKAYADEIDKS
                     KDFTRGYEQNVFAKSELNANKNTTKDKIKNEGAVKTSDTSLKLDNKSAISNGNEINQD
                     IKISNTPKNSSQGNNLVINNNEPTKEIKIANLEAQNSNQKKTNKVTNNYFGYYSFREA
                     PKTQIYTVKKGDTLSAIALKYKTTVSNIQNTNNIANPNLIFIGQKLKVPMTPLVEPKP
                     KTVSSNNKSNSNSSTLNYLKTLENRGWDFDGSYGWQCFDLVNVYWNHLYGHGLKGYGA
                     KDIPYANNFNSEAKIYHNTPTFKAEPGDLVVFSGRFGGGYGHTAIVLNGDYDGKLMKF
                     QSLDQNWNNGGWRKAEVAHKVVHNYENDMIFIRPFKKA"
     gene            5302..>5549
                     /locus_tag="NBI57_002089"
     CDS             5302..>5549
                     /locus_tag="NBI57_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan bridge formation glycyltransferase
                     FemA/FemB family protein"
                     /protein_id="HCD1823021.1"
                     /translation="MKFSTLSEEEFTNYTKKHFKHYTQSIELYNYRNKINHEAHIVGV
                     KNDKNEVIAACLLTEARIFKFYKYFYSHRGPLLDYFDA"
BASE COUNT         2116 a          732 c         1109 g         1592 t
ORIGIN      
        1 tatagttgaa ttaatgaatt tgttctataa ttaacaggtt atagatttta ataatgagaa
       61 aagaattgac gaaagtaagg tgaattgaat ggttattcaa tggtatccag gacatatggc
      121 gaaagccaaa agagaagtaa gtgaacaatt aaaaaaagta gatgtagtgt ttgaactagt
      181 agatgcaaga attccatata gttcaagaaa ccctatgata gatgaagtta ttaaccaaaa
      241 accacgtgtt gttatattaa ataaaaaaga tatgtctaat ttaaatgaga tgtcaaaatg
      301 ggaacaattt tttattgata aaggatacta tcctgtatca gtggatgcta agcacggtaa
      361 aaatttaaag aaagtggaag ctgcagcaat taaggcgact gctgaaaaat ttgaacgcga
      421 aaaagcgaaa ggacttaaac ctagagcgat aagagcaatg atcgttggaa ttccaaatgt
      481 tggtaaatcc acattaataa ataaactggc aaagcgtagt attgcgcaga ctggtaataa
      541 accaggtgtg accaaacaac aacaatggat taaagttagt aatgcattac aactattaga
      601 cacaccaggg atactttggc ctaaatttga agatgaagaa gtcggtaaga agttgagttt
      661 aactggtgcg ataaaagata gtattgtgca cttagatgaa gttgccatct atggattaaa
      721 ctttttaatt caaaatgatt tagcgcgatt aaagtcacat tataatattg aagttcctga
      781 agatgcagaa atcatagcgt ggtttgatgc gatagggaaa aaacgtggct taattcgacg
      841 tggtaatgaa attgattacg aagcagtcat tgaactgatt atttatgata ttcgaaatgc
      901 taaaatagga aattattgtt ttgatatttt taaagatatg actgaggaat tagcaaatga
      961 cgctaacaat taaagaagtt acgcagttga ttaatgcggt taatacaata gaagaattag
     1021 aaaatcatga atgcttttta gatgagcgaa aaggtgttca aaatgccata gctaggcgca
     1081 gaaaagcgtt agaaaaagaa caagctttaa aagaaaagta tgttgaaatg acttactttg
     1141 aaaatgaaat attaaaagag catcctaatg ctattatttg tgggattgat gaagttggaa
     1201 gaggaccttt agcaggtcca gtcgttgcat gcgcaacaat tttaaattca aatcacaatt
     1261 atttgggcct tgatgactcg aaaaaagtac ctattacgaa acgtctagaa ttaaatgaag
     1321 cactaaaaaa tgaagttact gcttttgcat atggtatcgc gacagctgaa gaaatagatg
     1381 aatttaatat ttataaagct actcaaatcg ccatgcagcg agctattgat ggattatcag
     1441 tacaaccaac gcatttattg atagacgcga tgacgcttga taatgcactg cctcaagtat
     1501 ctttaatcaa gggtgatgca agaagtgtgt ccattgcagc tgcaagtatc atggcgaagg
     1561 tttttcgtga tgattatatg acacagttat ctaaagatta tcctgaatac ggttttgaaa
     1621 aaaacgcggg ttatggtacc aaacaacatt tactagcaat cgatgatatt ggcattatga
     1681 aagagcatag aaaaagcttt gaacctataa aatcgttact gtaaatcgta aaattcaatt
     1741 aaatgataaa aaagtagcat ttaagtttac aatagcgctt acatttttta taattgtaag
     1801 ggaacttaac ctaagtaaca ggaggatgga agatgaatat ccacgagtat caaggtaaag
     1861 aaatatttcg ttcaatgggc gttgcagttc cagaaggacg agtagcattt actgctgaag
     1921 aagcggtgga gaaagcaaaa gaattaaatt ctgatgtata tgttgtaaaa gcacaaattc
     1981 atgctggagg tagaggtaaa gcaggcggag taaaaattgc taaatcttta tctgaggtag
     2041 aaacatatgc aaaagaatta ttagggaaaa ctttggtgac acatcaaact ggtccagaag
     2101 gtaaagaaat taaacgttta tatatcgaag aaggttgtgc tattcaaaaa gaatattacg
     2161 ttggattcgt tattgatcgt gcgactgacc aagtaacatt gatggcgtct gaagaagggg
     2221 gcactgagat tgaagaagtt gctgcgaaga ctcctgaaaa gatcttcaaa gaaactatcg
     2281 atccagtaat cggactttca ccattccaag caagacgaat tgcgtttaat attaatattc
     2341 ctaaagaatc tgttaacaaa gctgctaaat tcttattagc actttataat gtattcattg
     2401 aaaaagattg ttcaatcgta gaaatcaacc cattagttac aacagctgat ggtgatgtat
     2461 tggcattaga tgctaaaatt aattttgatg ataatgcatt attcagacat aaagatgttg
     2521 tagaattacg tgatttagaa gaagaagatc cgaaagagat tgaagcgtct aaacatgatt
     2581 tatcatacat tgcattagat ggtgacatcg gatgtatggt taatggtgca ggtttagcca
     2641 tggcaacaat ggatacgatt aatcatttcg gtggaaaccc agccaatttc ttagatgcag
     2701 gcggaagcgc tactagagaa aaagtaactg aagcatttaa aatcatttta ggtgatgaaa
     2761 atgttaaagg tatttttgta aacattttcg gtggcattat gaaatgtgat gttatcgcag
     2821 aaggtatcgt tgaagctgta aaagaagtag atttaacttt accactagtt gtacgcttag
     2881 aaggtacaaa tgttgagtta ggtaaaaaaa tcttaaaaga ctcaggatta gcaattgaac
     2941 cagcagcaac aatggctgaa ggtgcacaaa aaattgttaa actagtcaaa gaagcataag
     3001 gaaaggatgg gagcactaag atgagtgtat ttatagataa gaatactaaa gtaatggtac
     3061 aaggtattac agggtctact gcccttttcc atacaaaaca aatgcttgat tatggtacga
     3121 aaatagtagc aggtgtgacg cctggtaaag gtggtcaagt tgttgaaggc gttcctgttt
     3181 tcaacactgt tgaagaagct aaaaatgaaa ctggggcaac ggtttcagtc atttacgttc
     3241 cagcaccatt tgctgcagac tcaattttag aagcagctga tgcagactta gatatggtta
     3301 tttgtatcac tgaacatatt cctgtattag acatggttaa agttaaacgc tacttacaag
     3361 gtagaaaaac acgtttagtt ggtccgaact gtccaggtgt gattacagca gatgaatgta
     3421 aaattggtat tatgcctggc tatattcaca aaaaaggtca tgttggtgta gtatcacgtt
     3481 caggtacact aacatatgaa gcagtgcacc aattgactga agaaggtatt ggtcaaacta
     3541 cagctgttgg tattggtgga gacccagtca acggaacaaa ctttattgat gttttaaaag
     3601 cattcaatga agatgacgaa acgaaagcag ttgttatgat tggtgaaatc ggtggtacag
     3661 ctgaagaaga agcagctgaa tggattaaag cgaatatgac aaaaccagtt gtaggcttta
     3721 tcggtggaca aacagcacct cctggaaaac gtatgggaca tgctggtgca atcatttcag
     3781 gtggtaaagg tactgctgaa gagaaaataa aaacattaaa tagttgtggt gtgaaaacag
     3841 cggcaacacc ttcagaaatt ggttcaacat taattgaagc tgctaaagaa gcaggtattt
     3901 atgaatcatt attaacagtt aataaataaa gttaaaagat gatataaatg gctatagccc
     3961 attccatact ttataaaagt atcggaaatg ggctatttgt cgtgcaatga attttagtac
     4021 acttttcata ttttggaaag acaagttaat tattttacct cctacaatag ctttataaaa
     4081 caaagaaaat tttaattaaa atatatagtg cactacgtta taatgtttat atattattgt
     4141 aaggagtgtt ccatcatgaa taaacaacaa agtaaagtac gctattcaat tagaaaagtt
     4201 agtattggaa ttttgtcaat ttcaataggt atgtttttgg cattgggtat gtcgaacaaa
     4261 gcatatgcag atgaaattga taaatctaaa gattttacaa gagggtatga gcaaaatgta
     4321 ttcgcgaaat cagagttaaa tgctaataaa aatacgacaa aagacaaaat aaaaaatgaa
     4381 ggtgctgtta aaacatcgga cacaagttta aagttagaca acaaatcagc aatttcaaac
     4441 ggaaatgaaa ttaatcaaga tataaagatt tcaaatactc cgaaaaactc aagccaaggt
     4501 aacaatctag ttattaataa caatgaacct actaaagaaa ttaaaattgc aaacttggaa
     4561 gctcaaaatt ctaatcagaa gaaaacgaat aaagttacta ataattactt tggttactac
     4621 agttttagag aagctccaaa aacacaaatc tatactgtaa aaaaaggaga cacacttagt
     4681 gctatagcat taaaatacaa aactacagtt tcaaatattc aaaatacaaa taatatagca
     4741 aatcctaatt taatatttat tggtcaaaaa ttaaaagtgc caatgacacc attagtagaa
     4801 ccaaaaccaa aaacagtgtc ttcaaataat aaaagtaata gtaatagcag tacattaaat
     4861 tatttgaaaa cattagagaa tagaggatgg gatttcgacg gtagttatgg atggcaatgt
     4921 ttcgatttag ttaatgtata ttggaatcat ctttatggtc atggattaaa aggatatgga
     4981 gctaaagata taccatatgc aaataatttt aatagtgaag ctaaaattta tcacaacaca
     5041 ccaactttca aagctgaacc tggggactta gtggttttta gtggaagatt tggtggagga
     5101 tatggtcata cagctattgt cttaaatggt gattatgatg gaaaattaat gaagttccaa
     5161 agtttagatc aaaactggaa taatggtgga tggcgtaaag cagaggttgc acataaagtt
     5221 gttcataatt atgaaaatga tatgattttt attagaccat ttaaaaaagc ataatttaaa
     5281 tcgaaggcag gacattgaaa tatgaaattt tcaactttaa gtgaagaaga atttaccaac
     5341 tacaccaaaa agcacttcaa acattataca cagtctatag aattatataa ttatagaaat
     5401 aaaataaatc atgaagcaca tattgtggga gtgaagaatg ataaaaatga agttatagct
     5461 gcatgtttat taacagaggc acgaattttt aaattctaca aatatttcta ctctcataga
     5521 ggtcctttac ttgattattt cgatgctaa
//
LOCUS       DAHHSC010000159         5499 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.160, whole genome shotgun sequence.
ACCESSION   DAHHSC010000159 DAHHSC010000000
VERSION     DAHHSC010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 5499)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5499)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5499
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.160"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            234..698
                     /gene="accB"
                     /locus_tag="NBI57_002090"
     CDS             234..698
                     /gene="accB"
                     /locus_tag="NBI57_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HCD1823022.1"
                     /translation="MNFKEIKELIEILDKSTLTEINIEDTKGKVTLKKEKETEIITPQ
                     ISQMPVEAAAMPMPQAQSTDSNKTEAPKPTSDNHKTINAPMVGTFYKSPSPDEEAYVQ
                     VGDTVSNETTVCILEAMKLFNEIQAEISGEIVEILVEDGQMVEYGQPLFKVK"
     gene            698..2053
                     /gene="accC"
                     /locus_tag="NBI57_002091"
     CDS             698..2053
                     /gene="accC"
                     /locus_tag="NBI57_002091"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HCD1823023.1"
                     /translation="MKKVLIANRGEIAVRIIRACRDLGIQTVAIYSEGDKDALHTQIA
                     DEAYCVGPTLSKDSYLNIPNILSIATSTGCDGVHPGYGFLAENADFAELCEACQLKFI
                     GPSYQSIQKMGIKDVAKAEMIKANVPVVPGSDGLMKDVSEAKKIAKKIGYPVIIKATA
                     GGGGKGIRVARDEKELETGFRMTEQEAQTAFGNGGLYMEKFIENFRHIEIQIVGDSYG
                     NVIHLGERDCTIQRRMQKLVEEAPSPILDDETRREMGNAAVRAAKAVNYENAGTIEFI
                     YDLNDNKFYFMEMNTRIQVEHPVTEMVTGIDLVKLQLQVAMGDVLPYKQEDIKLTGHA
                     IEFRINAENPYKNFMPSPGKIEQYLAPGGYGVRIESACYTNYTIPPYYDSMVAKLIIH
                     EPTRDEAIMAGIRALSEFVVLGIDTTIPFHIKLLNNDIFRSGKFNTNFLEQNSIMNDE
                     G"
     gene            2068..2430
                     /locus_tag="NBI57_002092"
     CDS             2068..2430
                     /locus_tag="NBI57_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HCD1823024.1"
                     /translation="MVKVTDYSNSKLGKVEIAPEVLSVIASIATSEVEGITGHFAELK
                     ETNLEKVSRKNLSRDLKIESKEDGIYIDVYCALKHGVNISKTANKIQTSIFNSISNMT
                     AIEPKQINIHITQIVIEK"
     gene            2490..2879
                     /gene="nusB"
                     /locus_tag="NBI57_002093"
     CDS             2490..2879
                     /gene="nusB"
                     /locus_tag="NBI57_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HCD1823025.1"
                     /translation="MSRKESRVQAFQTLFQLEMKDSDLTINEAISFIKDDNPDLDFEF
                     IHWLVSGVKDHEPVLDETISPYLKDWTIARLLKTDRIILRMATYEILHSDTPAKVVMN
                     EAVELTKQFSDDDHYKFINGVLSNIKK"
     gene            2896..4233
                     /locus_tag="NBI57_002094"
     CDS             2896..4233
                     /locus_tag="NBI57_002094"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HCD1823026.1"
                     /translation="MSDYLSVSALTKYIKYKFDQDPHLQSVLIKGELSNFKKHSSGHL
                     YFNVKDKESVISAMMFKGSASKLNFEPKEGDEVLLEARVSVFERRGNYQIYVNKMQLD
                     GIGNLYQKLEALKKKLTEEGCFDKANKKSIPKFPKKIAVLTASTGAAIRDIHSTINSR
                     FPLAEQIQISTLVQGEKAKDDIIEKIEYADSLGVDTIIVGRGGGSIEDLWNFNEEAVV
                     RAIYNCKTPIISAVGHETDFTLSDFAADIRAATPTQAAVIATPDQYELLQQIQQYQFT
                     LTRFIKKHLEQQRKHVEHLSSYYKFKQPTLLYDQQIQRRDDLEKRLKQQIQATFEQQR
                     HRLMLLQQRYNLKALLSSVNQEQQNNLQLTNQLVKLLNSKILSYKNDLKNKVENLNNL
                     SPTNTMLRGYAIVNKKDEVITSTKDLTENDQLTLTMKDGLVDAKVTKVRCNND"
     gene            4226..4456
                     /locus_tag="NBI57_002095"
     CDS             4226..4456
                     /locus_tag="NBI57_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HCD1823027.1"
                     /translation="MTKETQSFEEMMQELEQIVQKLDNETVSLEESLDLYQRGMKLSA
                     ACDTTLKNAEKKVNDLIKEEAEDVKNDESTDE"
     gene            4434..5315
                     /locus_tag="NBI57_002096"
     CDS             4434..5315
                     /locus_tag="NBI57_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HCD1823028.1"
                     /translation="MTNLPMNKLIDEVNNELSVAINKSVMDTQLEESMLYSLNAGGKR
                     IRPVLLLLTLDSLNTEYELGVKSAIALEMIHTYSLIHDDLPAMDNDDYRRGKLTNHKV
                     YGEWTAILAGDALLTKAFELISSDDRLTDEVKIKVLQRLSIASGHVGMVGGQMLDMQS
                     EGQPIDLETLEMIHKTKTGALLTFAVMSAADIANVDDATKEHLESYSYHLGMMFQIKD
                     DLLDCYGDEAKLGKKVGSDLENNKSTYVSLLGKDGAEDKLTYHRDAAVDELTQIDEQF
                     NTKHLLEIVDLFYSRDH"
BASE COUNT         2099 a          758 c         1039 g         1603 t
ORIGIN      
        1 taataaaaat aacattgata tgtttaactg aatgcataaa atttttaatc aagagttaag
       61 atgagtcaat gttaagtaat aaatacacaa gtgtttttaa tttaaaatgt ggttttatta
      121 taactttggg atagacttat aatgtcgcca gcaatatgaa catgcttgaa ttgaagagtt
      181 gtctcaagta aaatagacgg gtagatgaaa acaaactgaa ggagtcagta ataatgaact
      241 ttaaagaaat caaagaatta attgaaattc tggataaatc aactttaacg gaaatcaata
      301 ttgaagatac taaaggcaaa gtgacgctta agaaagaaaa agaaactgag attatcacgc
      361 cacaaatctc acaaatgcca gttgaagctg cggcaatgcc tatgcctcaa gcacaatcaa
      421 ctgatagcaa taaaactgaa gctccaaagc caacttcaga taatcacaaa acaattaatg
      481 cacctatggt aggtacattt tacaaatcgc catctccaga cgaagaagca tatgtgcaag
      541 ttggggacac tgtttcaaat gaaacaacag tgtgtatttt agaggcaatg aaactattta
      601 atgaaattca agcagaaatt tcaggtgaaa ttgttgaaat cttagtagaa gacggacaaa
      661 tggtagagta tggccaaccg ttatttaagg tgaaataatg aaaaaggttt taattgcaaa
      721 ccgcggtgaa atcgcagtta ggattattcg cgcttgtcgt gatttaggca tccaaactgt
      781 tgcaatctat tctgaagggg ataaagatgc gctacatact caaattgctg atgaagcata
      841 ttgcgtaggt cccactttgt ctaaagattc atatttaaat attccgaaca tcttatctat
      901 tgcaacttct acaggttgtg atggcgttca tccgggttat ggctttttag ctgaaaatgc
      961 tgactttgca gaattatgcg aagcatgcca attgaagttc attggaccaa gttatcaatc
     1021 tatccaaaaa atgggtatca aagatgttgc taaggcagaa atgatcaaag ccaatgttcc
     1081 agttgttcct ggtagtgacg gtttaatgaa agacgtctca gaagctaaga aaatcgctaa
     1141 gaaaattggc tatccggtca tcattaaagc tactgctggt ggtggcggaa aaggtatccg
     1201 tgttgctcgt gatgaaaaag aacttgaaac tggcttccga atgacagaac aagaagctca
     1261 aactgcattt ggtaatggtg gactttatat ggagaaattc atcgaaaact tccgccatat
     1321 tgaaatccaa attgttgggg acagctatgg taatgtaatt catttaggag aacgtgattg
     1381 tacaattcaa agacgtatgc agaaattagt ggaagaagca ccttccccaa ttttagatga
     1441 tgaaacacgt cgtgaaatgg gaaatgccgc agttcgtgca gcgaaagctg taaattatga
     1501 aaatgcggga acaattgagt ttatatatga tttaaatgat aataaatttt attttatgga
     1561 aatgaataca cgtattcaag tagaacatcc tgtaactgaa atggtaacag gaattgattt
     1621 agttaaatta caattacaag ttgctatggg tgacgtgtta ccgtataaac aagaagatat
     1681 taaattaaca ggacacgcaa ttgaatttag aattaatgct gaaaatcctt acaagaactt
     1741 tatgccatca ccaggtaaaa ttgagcaata tcttgcacca ggtggatatg gtgttcgaat
     1801 agagtcagca tgttatacta attatacgat accgccatat tatgattcga tggtagcgaa
     1861 attaatcata catgaaccga cacgagatga agcgattatg gctggcattc gtgcattaag
     1921 tgaatttgtg gttcttggta ttgatacaac tattccattc catattaaat tattgaataa
     1981 cgatatattt agaagcggta aatttaatac aaacttttta gagcaaaata gcattatgaa
     2041 tgatgaaggt taataggagg tcaatccatg gtcaaagtaa ctgattattc aaattcaaaa
     2101 ttaggtaaag tagaaatagc gccagaagtg ctatctgtta ttgcaagtat agctacttcg
     2161 gaagtcgaag gcatcactgg ccattttgct gaattaaaag aaacaaattt agaaaaagtt
     2221 agtcgtaaaa atttaagccg tgatttaaaa atcgagagta aagaagatgg catatatata
     2281 gatgtatatt gtgcattaaa acatggtgtt aatatttcaa aaactgcaaa caaaattcaa
     2341 acgtcaattt ttaattcaat ttctaatatg acagcgatag aacctaagca aattaatatt
     2401 cacattacac aaatcgttat tgaaaagtaa tgtcatacct aattcagtaa ttaaataaag
     2461 aaaaatacaa acgtttgaag gagttaaaaa tgagtcgtaa agaatcccga gtgcaagctt
     2521 ttcaaacttt atttcaatta gaaatgaagg acagtgattt aacgataaat gaagcgataa
     2581 gttttattaa agacgataat ccagatttag actttgaatt tattcattgg ctagtttctg
     2641 gcgttaaaga tcacgaacct gtattagacg agacaattag tccttattta aaagattgga
     2701 ctattgcacg tttattaaaa acggatcgta ttattttaag aatggcaaca tatgaaatat
     2761 tacacagtga tacacctgct aaagtcgtaa tgaatgaagc agttgaatta acaaaacaat
     2821 tcagtgatga tgatcattat aaatttataa atggtgtatt gagtaatata aaaaaataaa
     2881 attgagtgat gttatatgtc agattattta agtgtttcag ctttaacgaa atatattaaa
     2941 tataaatttg atcaagatcc acatctccaa tctgtattga ttaaaggtga gctttcaaat
     3001 ttcaaaaagc attcaagtgg tcatttatac tttaatgtga aagataaaga gagcgttata
     3061 agtgccatga tgttcaaagg tagtgcttct aaattaaact tcgaacctaa agaaggagat
     3121 gaagtcttat tagaagcacg tgtttctgtt tttgaacgtc gtggaaacta tcaaatttat
     3181 gtaaataaaa tgcaattaga tggtataggg aacttatatc aaaaattaga agcattaaag
     3241 aaaaaattaa ctgaagaagg ttgttttgat aaagcgaata agaaatcaat acctaagttt
     3301 cctaaaaaaa ttgcagtttt aacagcgagt acaggtgccg caattcgaga tatccattca
     3361 acgataaata gtcgttttcc attagctgaa caaatacaaa ttagtacttt ggttcaaggt
     3421 gaaaaagcaa aagacgacat tattgaaaaa attgaatatg cagatagttt aggtgtagat
     3481 accattattg taggtcgagg tggtggatcc atagaagatt tatggaattt caacgaagaa
     3541 gctgtcgtcc gtgcaatata taattgtaag acaccgatta tatcagcagt aggtcatgaa
     3601 acagacttta cattaagtga ttttgctgca gacatcagag ctgcgactcc aactcaagct
     3661 gctgttattg caacaccaga ccaatatgaa ttgctgcaac aaatacagca atatcagttt
     3721 acattgactc gtttcatcaa gaaacattta gaacaacaac gtaaacatgt tgaacatttg
     3781 tcatcgtatt acaagtttaa acaaccaact ttattgtatg atcaacagat acaacgtcga
     3841 gatgatttag aaaagagact gaaacaacaa attcaggcaa cttttgaaca acagagacat
     3901 cgattaatgc tgttgcaaca acgttataat ttaaaagcat tactaagtag tgttaatcaa
     3961 gaacaacaaa ataatcttca attgacaaac caacttgtta aattattgaa ttctaagata
     4021 ttaagttata aaaatgattt gaaaaataaa gtagaaaact taaacaattt aagtccaact
     4081 aatacaatgt tgcgtggata tgcaattgtt aataaaaaag acgaagtcat tacgagtact
     4141 aaagatttaa cagaaaatga tcaattgacg ttaacaatga aagatggctt agtagatgca
     4201 aaagttacga aagtaaggtg taataatgac taaagaaacg caaagttttg aagaaatgat
     4261 gcaagaatta gagcaaattg ttcaaaaatt agataatgaa acagtatctt tagaggaatc
     4321 attagattta tatcaacgtg gtatgaaact atcagcagct tgtgacacaa ctttaaaaaa
     4381 tgccgaaaaa aaggtgaatg acttaataaa agaagaagct gaggatgtaa aaaatgacga
     4441 atctaccgat gaataaatta atagatgaag tcaataatga attatcggtt gcgataaata
     4501 aatcagtaat ggatactcag ctagaagaaa gcatgttgta ttcattaaat gctggaggta
     4561 aacgcatccg accagttctg ttattactca ctttagattc actaaatacc gagtatgagt
     4621 taggtgtgaa gagcgcaatt gcactagaaa tgattcatac atattcactt attcatgatg
     4681 acctaccagc gatggataat gatgattatc gacgagggaa attaacaaat cataaagtat
     4741 atggtgagtg gactgcgata ttagcaggtg atgctttatt aactaaagca tttgaactta
     4801 tttcaagtga tgatagatta actgatgaag taaaaataaa agttctacaa cggctgtcaa
     4861 tagcaagtgg tcatgttgga atggtcggcg gtcaaatgtt agatatgcaa agcgaaggcc
     4921 aaccaattga tcttgaaact ttggaaatga tacacaaaac aaaaacagga gcattattaa
     4981 cttttgcggt tatgagtgca gcagatatcg ctaatgtcga tgatgcaact aaagaacatt
     5041 tagaaagtta tagttatcat ttaggtatga tgttccagat taaagatgat ttattagact
     5101 gctatggtga tgaagcaaag ttaggtaaaa aagtgggcag cgatcttgaa aataataaaa
     5161 gtacgtacgt gagtttatta gggaaagatg gcgcagaaga taaattgact tatcatagag
     5221 acgcagcagt ggatgaacta acgcaaattg atgaacaatt caatacaaaa cacttattag
     5281 aaatcgttga tttattttat agcagggatc actaaaagca aatatatcga ttagcaacaa
     5341 ttgtaaagtt tgaaatgtct caaaaggcat attcaactga cgttgtttgt taatcgattt
     5401 ttctcatgtt gaaacttaaa attatgttga agactttcat ttaaaaaata gaattttcaa
     5461 tttctatacg acaatattcg tatctgtgac atcaatgtg
//
LOCUS       DAHHSC010000160         5444 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.161, whole genome shotgun sequence.
ACCESSION   DAHHSC010000160 DAHHSC010000000
VERSION     DAHHSC010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 5444)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5444)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5444
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.161"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            complement(<1..430)
                     /gene="deoC"
                     /locus_tag="NBI57_002097"
     CDS             complement(<1..430)
                     /gene="deoC"
                     /locus_tag="NBI57_002097"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HCD1823029.1"
                     /translation="MKFEKYIDHTLLKPESTRTQIDQIIDEAKAYNFKSVCVNPTHVK
                     YAAERLADSEVLVCTVIGFPLGASTTATKAFETEDAIQNGADEIDMVINIGALKDGRF
                     DDVQQDIEAVVKAAKGHTVKVIIETVLLDHDEIVKASELTK"
     gene            complement(511..1863)
                     /gene="tet(38)"
                     /locus_tag="NBI57_002098"
     CDS             complement(511..1863)
                     /gene="tet(38)"
                     /locus_tag="NBI57_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001100300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(38)"
                     /protein_id="HCD1823030.1"
                     /translation="MNVEYSKIKKAVPILLFLFVFSLVIDNSFKLISVAIADDLNISV
                     TTVSWQATLAGLVIGIGAVVYASLSDAISIRTLFIYGVILIIIGSIIGYIFQHQFPLL
                     LVGRIIQTAGLAAAETLYVIYVAKYLSKEDQKTYLGLSTSSYSLSLVIGTLSGGFIST
                     YLHWTNMFLIALIVVFTLPFLFKLLPKENNTNKAHLDFVGLILVATIATTVMLFITNF
                     NWLYMIGALIAIIVFALYIKNAQRPLVNKSFFQNKRYASFLFIVFVMYAIQLGYIFTF
                     PFIMEQIYHLQLDTTSLLLVPGYIVAVIVGALSGKIGEYLNSKQAIITAIILIALSLI
                     LPAFAVGNHISIFVISMIFFAGSFALMYAPLLNEAIKTIDLNMTGVAIGFYNLIINVA
                     VSVGIAIAAALIDFKALNFPGNDALSSHFGIILIILGLMSIVGLVLFVILNRWTQSEK
                     "
     gene            complement(1870..2577)
                     /gene="deoD"
                     /locus_tag="NBI57_002099"
     CDS             complement(1870..2577)
                     /gene="deoD"
                     /locus_tag="NBI57_002099"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HCD1823031.1"
                     /translation="MKSTPHIKPMNDVEIAETVLLPGDPLRAKFIAETYLDDVEQFNT
                     VRNMFGFTGTYKGKKVSVMGSGMGMPSIGIYSYELIHTFGCKKLIRVGSCGAMQENID
                     LYDVIIAQGASTDSNYVQQYQLPGHFAPIASYQLLEKAVETARDKGVRHHVGNVLSSD
                     IFYNADTTASERWMRMGILGVEMESAALYMNAIYAGVEALGVFTVSDHLIHETSTTPE
                     ERERAFTDMIEIALSLV"
     gene            2813..3568
                     /locus_tag="NBI57_002100"
     CDS             2813..3568
                     /locus_tag="NBI57_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCD1823032.1"
                     /translation="MEVVYVTQTQPKFLTIYNTLYKEIQIGKYPSGHALPTEKELCAR
                     FDVSRMTLRQAIKLLAEDGIVESTRGKGHFVIPQTNAQHASSMDQFKHPLDQILLARV
                     SMSSINYRVDLESEYTNHLFANHPSAVIAMERYYQKKDNHSKQADAFCFTFIPLNVID
                     TFKVNTQNEDDMKTFVEQTIYSNAYQSDLKMSITKAPRFKNHSHVFDGDTHCWLIIET
                     LYAQTPYPIMINKWYIPQEISELTLTRIRQSDY"
     gene            complement(3759..4484)
                     /gene="sasD"
                     /locus_tag="NBI57_002101"
     CDS             complement(3759..4484)
                     /gene="sasD"
                     /locus_tag="NBI57_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_070002951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell-wall-anchored protein SasD"
                     /protein_id="HCD1823033.1"
                     /translation="MKKLATVGSLIVTSTLVFSSMPFQNAHADTTSMNVPNKQSQNVQ
                     NHRPYGGVVPQGMTQAQYTELEKTLPQLSAGSNMQDYNMKLYDATQNIADKYNVIITT
                     NVGVFKPHAVRDMNGHALPLTKDGNFYQTNVDANGVNHGGSEMVQNKTGHMSQQDHMN
                     QNTHMNQQPQIQQGHMQSSNHQMMSPKANMHSSNHQMNQSNKKVLPAAGESMTSSILT
                     ASIAALLLVSGLFLAFRRRSTNK"
     gene            complement(4851..>5444)
                     /locus_tag="NBI57_002102"
     CDS             complement(4851..>5444)
                     /locus_tag="NBI57_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HCD1823034.1"
                     /translation="FKLPNLPYAYDALEPYIDQRTMEFHHDKHHNTYVTKLNATVEGT
                     ELEHQSLADMIANLDKVPEAMRMSVRNNGGGHFNHSLFWEILSPNSEEKGGVIDDIKA
                     QWGTLDEFKNEFANKATTLFGSGWTWLVVNDGKLEIVTTPNQDNPLTEGKTPILLFDV
                     WEHAYYLKYQNKRPDYMTAFWNIVNWKKVDELYQAAK"
BASE COUNT         1759 a          983 c          837 g         1865 t
ORIGIN      
        1 cttttgttaa ttcactcgct tttacaattt cgtcatggtc caacaatacc gtctcaataa
       61 tcacttttac tgtgtgacct ttcgcagctt taaccactgc ttcaatgtct tgttgtacat
      121 catcaaaacg tccatctttt aatgcgccga tgttgatgac catgtcaatt tcatctgcac
      181 cattttgaat tgcatcttct gtttcaaatg ctttcgttgc agttgtcgac gcacctaatg
      241 ggaatcctat taccgtacaa acgagcacct ctgaatcagc tagtcgctct gctgcatatt
      301 taacatgcgt tggattcaca catacagatt taaaattgta tgctttcgct tcatcgatga
      361 tttgatcgat ttgcgtacgt gttgactcag gcttcaataa agtgtgatct atatatttct
      421 caaatttcat acttactact cctcgtgtta tataatctct ttatttaatt ttactataaa
      481 tacgaatata tctcgcgaat ttatatctat ttatttttca gattgtgtcc aacgatttaa
      541 gatgacgaat aaaactaatc cgacaatact cattaaacct aaaataatta aaataatacc
      601 gaaatgtgaa cttaatgcat cattgcctgg gaaatttaat gctttaaaat cgattagagc
      661 cgcagcaatc gcaataccta cagataccgc cacattaata attaaattat aaaaaccaat
      721 agccacacct gtcatattaa gatctattgt tttaatggct tcgttaagta aaggtgcata
      781 cattaaagca aagctacctg caaagaatat catagaaatg acgaagattg aaatgtgatt
      841 acctactgca aatgcaggta aaatcaagct cagtgctatt aaaataattg ctgtgataat
      901 cgcttgtttt gaattcagat attcgccgat tttaccactt aatgcaccaa caatgactgc
      961 tactatataa cccggtacta ataacagtga tgttgtgtct agttgcagat gataaatttg
     1021 ctccattatg aatgggaacg taaaaatata acccaattgg atagcataca ttacaaatac
     1081 tataaataaa aatgaagcat aacgtttatt ttggaaaaat gatttattta ctaatggacg
     1141 ttgcgcattt ttaatatata gcgcaaaaac gataatcgca attaaggcac caatcatata
     1201 taaccaatta aagttcgtaa taaacagcat gactgttgta gcaatagttg ccactagaat
     1261 taagccaaca aaatctaaat gagctttatt cgtattattt tcttttggta ataatttaaa
     1321 taggaatggc aacgtaaata ctacgattaa tgcaattaaa aacatatttg tccagtgtaa
     1381 atacgtagaa ataaatccac ctgataatgt accgataact aatgacaagg aataactgct
     1441 cgtacttaag ccaaggtaag tcttctggtc ctctttagaa agatactttg caacatatat
     1501 cacatataat gtctctgcag cagctaaacc ggcagtttga ataatacgtc caactaaaag
     1561 taatgggaat tgatgttgga aaatgtaacc aataattgat ccgataatga ttaatatcac
     1621 gccataaata aatagtgtgc gtatactaat ggcatcagat aatgaagcgt atactacagc
     1681 gccaatacca attactaaac cggctaatgt cgcttgccaa cttactgtcg ttacagatat
     1741 gtttaagtca tcagcaatgg ctacagaaat caatttaaat gagttgtcta taaccaaact
     1801 gaatacaaat aagaataata aaataggtac tgctttcttt attttagaat attcaacatt
     1861 cataatcatc tacaccaatg acagtgcaat ttcaatcata tctgtaaatg cacgttccct
     1921 ttcctcaggt gttgttgacg tttcatgaat taaatgatcg ctcactgtga acacacctaa
     1981 tgcttcgaca ccagcgtaaa ttgcattcat gtataatgca gctgattcca tttctacacc
     2041 taaaataccc atacgcatcc aacgttcact cgctgttgta tccgcgttat agaaaatatc
     2101 acttgataac acattaccta catgatgacg tacacctttg tcacgtgctg tttcaactgc
     2161 tttttctaat aattgataag aagcaattgg cgcaaaatga cctggtaatt gatattgttg
     2221 aacgtaattt gaatcagtag aggcaccttg tgcaataatc acatcatata aatcaatgtt
     2281 ttcttgcatc gcgccacaag agccaacgcg aattaatttt ttacaaccaa atgtatgaat
     2341 taattcataa gagtaaatgc caatagatgg catacccata cctgaaccca tgacagaaac
     2401 ttttttacct ttatatgttc cggtaaaacc aaacatgttt cgcactgtat tgaactgttc
     2461 cacatcatcc aaataagttt ctgcaatgaa cttagctctt aacggatctc ctggcaatag
     2521 aaccgtttct gcaatttcga cgtcattcat tggtttaatg tgtggtgttg atttcataaa
     2581 atattcctcc caatatttgt atagtgattt gtctatttgt ctaatcatgt atataataat
     2641 attttttaaa agcgctttca agcattacat tttgacaata ttaagaacat tttacagtta
     2701 cagctactaa ccttgtataa aactaatttt caaagcctat ctatatttaa tttttataat
     2761 ttttccaatt cgagtaaaaa ttattgtatg atgagagtga ttaagtaact ttatggaggt
     2821 tgtctacgtg acgcaaacac aaccaaaatt tttaacgatt tataatacgt tatataaaga
     2881 aattcaaata ggaaaatatc cgagtggcca tgcattacct actgaaaagg aactttgtgc
     2941 gcgctttgat gtcagtcgta tgactttgcg ccaagctata aaattgttag ccgaagatgg
     3001 catcgtagag agtacaagag ggaagggaca ctttgttatt ccacaaacaa atgcccaaca
     3061 cgcttcaagt atggatcaat ttaagcatcc acttgatcaa atattgctag ctcgcgtatc
     3121 tatgtcatca attaattatc gtgttgattt agaaagtgaa tatacaaatc atttgtttgc
     3181 aaaccatcct tctgccgtca ttgcaatgga acgctattat caaaaaaagg acaatcattc
     3241 taagcaagct gatgcatttt gctttacctt tataccgtta aatgtcattg acacatttaa
     3301 agtgaataca caaaacgaag acgacatgaa gacattcgtt gagcaaacga tttatagcaa
     3361 tgcatatcaa tcagatttaa aaatgtctat taccaaagcg ccccgcttca aaaatcatag
     3421 tcacgtgttt gatggtgata cacattgttg gctgattatt gaaacactgt acgcacaaac
     3481 accttatcca attatgatta ataagtggta tattccacag gaaatatctg aattaacact
     3541 gacacgtata agacaatcgg attattaaaa ttattaaaat ataagaaatt ttttgatgac
     3601 tatttaggga gttggtaaga aatggaaaag cttgttacaa gcgcaatttc attcagtcaa
     3661 ctactgccaa tataacatta gcgagcctag gacattgatt tatgtcctag gctcgctcag
     3721 ataatcacac gatttttcta ttattaatcg tattatgttt atttatttgt tgaacgtcgt
     3781 ctaaatgcta agaataaccc agatactaat agtagtgcgg caatacttgc agtaagaata
     3841 cttgatgtca tactttcacc agcagctggt aaaacttttt tgttactttg gttcatttga
     3901 tgatttgatg aatgcatatt tgcttttgga ctcatcattt gatggtttga tgattgcata
     3961 tgaccttgtt gtatttgtgg ctgttggttc atgtgtgtgt tctgattcat atggtcttgt
     4021 tgactcatat gacctgtttt attttgcacc atttcactac caccatgatt aacaccattt
     4081 gcatctacat tcgtttgata aaaattgcca tcttttgtta aaggtaacgc atggccattc
     4141 atatctctaa cagcatgtgg tttaaatacc cctacattag ttgtaattat cacattgtat
     4201 ttatcagcaa tattttgcgt cgcatcatac aatttcatat tatagtcttg catattactg
     4261 ccagcgctta attggggtaa agttttctct aattcagtat attgtgcttg cgtcattcct
     4321 tgtggtacta ctccgccata agggcgatga ttttgtacat tttggctttg tttattcggc
     4381 acattcattg aagtcgtgtc ggcatgcgca ttttgaaaag gcatacttga gaatactaaa
     4441 gtgcttgtta caattaaaga acctactgtt gctaattttt tcattgtctt ttcccttata
     4501 ttacaatttg attacattta cattaccata tgattacaaa agaaatgcaa caaaattttt
     4561 gaatcgttac aattttttat aaaaatttca ctttagattc acaataatta cttattttgt
     4621 caatttattt aatgtcaata tgttgattaa ttaatagtgt tgtctgatgt atataacatt
     4681 taggtcatca ttatagtcaa caataataag gtatttcgag ttgaaattta tcttattatt
     4741 ttgccacttt tacgtgctat cccattacac aaaaacaaat aagtaaagat attaagtaca
     4801 aaaaagtgga acaccttgta aatgctccac ctcaattata ttaagttata ttattttgct
     4861 gcttggtata attcatcaac ttttttccag ttaacaatat tccaaaatgc agtcatatag
     4921 tctggacgtt tattttgata tttcagatag taggcatgct cccaaacatc aaatagtaag
     4981 attggtgttt tgccttctgt taatggatta tcttggtttg gcgttgtcac aatttctaat
     5041 tttccatcat taacaactaa ccaagtccaa cctgatccaa ataatgttgt tgctttattt
     5101 gcaaattcat ttttaaattc atctaaagtg ccccactgcg ctttgatgtc atctattacg
     5161 ccaccttttt cttcagaatt aggtgatagt atttcccaga ataatgaatg gttaaaatga
     5221 ccaccgccat tattacggac tgacatcctc atcgcttccg gtaccttgtc taagttagca
     5281 atcatatccg ctagtgattg atgctctaac tctgttcctt caactgttgc gtttaatttc
     5341 gtcacgtacg tattgtgatg tttgtcgtga tgaaactcca ttgttctttg atctatatat
     5401 ggttccaatg catcatatgc atatggtaaa tttggtaatt taaa
//
LOCUS       DAHHSC010000161         5437 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.162, whole genome shotgun sequence.
ACCESSION   DAHHSC010000161 DAHHSC010000000
VERSION     DAHHSC010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 5437)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5437)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5437
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.162"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            <1..726
                     /locus_tag="NBI57_002103"
     CDS             <1..726
                     /locus_tag="NBI57_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E domain-containing protein"
                     /protein_id="HCD1823035.1"
                     /translation="VNELTEFGGEKIPQGHKDIFDPNLPTDQTEKVPGKPGIKNPDTG
                     KVIEEPVDDVIKHGPKTGTPETKTVEIPFETKREFNPKLQPGEERVKQEGQPGSKTIT
                     TPITVNPLTGEKVGEGQPTEEITKQPVDKIVEFGGEKPKDPKGPENPEKPSRPTHPSG
                     PVNPNNPGLSKDRAKPNGPVHSMDKNDKVKKSKIAKESVANQEKKRAELPKTGLESTQ
                     KGLIFSSIIGIAGLMLLARRRKN"
     gene            1110..2311
                     /locus_tag="NBI57_002104"
                     /pseudo
     CDS             1110..2311
                     /locus_tag="NBI57_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501256.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            2283..2516
                     /locus_tag="NBI57_002105"
     CDS             2283..2516
                     /locus_tag="NBI57_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1823036.1"
                     /translation="MLRSLCKQNRILINAIKVGIEMKYKISLAYNLAIIIGSLIILCI
                     LISRGYDIYVILIPILTILASLINLICDIKKHK"
     gene            complement(2624..2932)
                     /locus_tag="NBI57_002106"
     CDS             complement(2624..2932)
                     /locus_tag="NBI57_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1823037.1"
                     /translation="MTVNIVFSIVFCISMVILGIYVAITKDFTLISYINQTTIADKHK
                     NQIAYIFTLCISLSAVFLMSSILCFEYDFIALSFLFLTIALLLIALFYVCFYKITKYP
                     "
     gene            complement(3076..3342)
                     /locus_tag="NBI57_002107"
     CDS             complement(3076..3342)
                     /locus_tag="NBI57_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1823038.1"
                     /translation="MKGSKQILLIMGIISLIVLFIFTLFIMAQYAKHYEQKSDSSNAH
                     TLNSSSAIIEQHTMSNLASLDLYAPVRNITSSRDIHPIYFMTKD"
     gene            complement(3626..5434)
                     /locus_tag="NBI57_002108"
     CDS             complement(3626..5434)
                     /locus_tag="NBI57_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HCD1823039.1"
                     /translation="MHFHTHLLYNTCEKYCLGAVFFYFWGVFLYNSLHKCKGFSFHVL
                     LSLTEILKGCLATMDKELWIERANDSLVKHFYEQQSDIEQREGFESKLTFGTAGIRGK
                     FGLGEGRLNKFTIEKLALGLARYLNAQTNSPTIVIHYDIRHLSTEFAQIIANVLANHQ
                     ITVYLPDTYKTTPELSFAVRNLNTTAGIMITASHNPKDYNGIKVYGSDGAQLSTDASE
                     LASRYIEEVGDPLQIDIPISKQNTSYIKPFPKSVTDDYMKHIQNMIGYIPKSDLQVVF
                     TSLHGTSVPIVPELLKSLNFNQFNLVDAQCKPDPNFSSVQSANPEDHRAFDQAVELAN
                     KSHADLLISTDPDADRLGIAERDAHGHITYFNGNQIGALLLNYRIQQTSQLRHRLMIQ
                     SIVSSELTKSLARYNNVKYKEVLTGFKFIAQEIRQLDDHQNMIFAFEESYGFLSEPFV
                     RDKDAVQIVPLIIKYASELKLYGKTLKDELEQIYQTVGRHEDTLFSHTLEGLEGKKKI
                     ESIMTHFRSNPPQEIQGLKVKAIEDYLTSEVYHLDKDTTSQINSSKSNVIRVLFDEGF
                     IALRPSGTEPKIKLYVSLKCPDFDDVAQKINAMIFS"
BASE COUNT         1921 a          795 c          905 g         1816 t
ORIGIN      
        1 gttaatgaat taacagaatt cggtggcgag aaaataccgc aaggtcataa agatatcttt
       61 gatccaaact taccaacaga tcaaacggaa aaagtaccag gtaaaccagg aatcaagaat
      121 ccagacacag gaaaagtgat cgaagagcca gtggatgatg tgattaaaca cggaccaaaa
      181 acgggtacac cagaaacaaa aacagtagag ataccgtttg aaacaaaacg tgagtttaat
      241 ccaaaattac aacctggtga agagcgagtg aaacaagaag gacaaccagg aagtaagaca
      301 atcacaacac caatcacagt gaacccatta acaggtgaaa aagttggcga gggtcaacca
      361 acagaagaga tcacaaaaca accagtagat aagattgtag agttcggtgg agagaaacca
      421 aaagatccaa aaggacctga aaacccagag aagccgagca gaccaactca tccaagtggc
      481 ccagtaaatc ctaacaatcc aggattatcg aaagacagag caaaaccaaa tggcccagtt
      541 cattcaatgg ataaaaatga taaagttaaa aaatctaaaa ttgctaaaga atcagtagct
      601 aatcaagaga aaaaacgagc agaattacca aaaacaggtt tagaaagcac gcaaaaaggt
      661 ttgatcttta gtagtataat tggaattgct ggattaatgt tattggctcg tagaagaaag
      721 aattaaaata attcataatt taaataatag ttgatttgca ttcactatat ttagtttgtt
      781 aaaaacaacc tagaatatga tgagaatgat atacacccca aaagttggat tgaaagacct
      841 aactttaagg ggtgtatttt tgttataaat ttgatttttt gataatattt tcatatttaa
      901 tataatcata attagtagaa tataacctct gagtagtcat tttggggggt atgtatggtg
      961 tttcaagtag tatgtatatt tcgaaataat tgatatttaa ctgacttaat tttattagaa
     1021 ttaattgcgc tttggataaa gagagtttag aagaaggtct aaaaaaggct ggtggatgca
     1081 ttcaattatt tttatgagag gtgataaata tgttgtactt agaagttttg aaaaatagaa
     1141 actttacata tttattgatt ggtaatttct tacgtcggag ttgctttgtg ctgttttcgt
     1201 tacaaataat ttggtttact gttgaattga caaaccaatc atctttaaaa ttatcaatga
     1261 tggttatgag tcaaacactt ccatttatta tatttggtat tttcggtggc gcatattctg
     1321 ataaacataa taaaaagaaa atactttatc tatctgatct tatactaagt ttgattataa
     1381 taattattcc tttacttgca ataacatcaa atttaaatta tctgacgctt ctaaccatat
     1441 ctacagctat caccattatt aactgctata cggatcctgc gtttagggca attcttcctg
     1501 aaattattga tgaggagcat ttagcaacaa gtaatgcgtt aatcgatagt ttgcaaagag
     1561 gctcaaatat tattttacct gctttaattg gtgtcattgt aatactagtt ggtaatgttg
     1621 gcgtcttttt tatttgcagt atattgctat ttttaggatt tatttttaat gcacttttaa
     1681 aatatacaaa taacaacatg attgatagac actcaaaaga agatttttct gaaacttggg
     1741 aattcttgaa acaatctaaa gaaattccat ttatcataat catccaattt gcctgcatat
     1801 tgattaatac tggcctttgg cgtgttgcat taccattgtt tatttccaat atattaaaag
     1861 agggcgttgg tgtatatgga ctagctacat catgtttagg tatagcatct ttattaatgt
     1921 cattgattat gggtttacta tcagaaaaac gattaatctt taaatttagt attggtgttt
     1981 tagtttgggg gattggctta tcgataatta atgtgtttcc aagtgtggcg atactatata
     2041 taggtgcaac attagtggga cttggacaat ctatagaggg actaacaaga tcagttgcca
     2101 ttcaaataaa aacgccaaat catttgatgg ggaaagtatt tagtatctca tctacatcga
     2161 attacgcagc ggatacatta tcttaggact gataagttta attatacctt tactaagctt
     2221 aagtaatata tttatatcag gaggtgcaat tatttcaatg cttagcttag ctgggttaaa
     2281 gtttgttaag aagtctatgc aaacaaaata gaattttaat caatgctatt aaggtgggaa
     2341 ttgaaatgaa atataaaatt agtttggctt ataatttagc aattataata gggtcgctaa
     2401 ttatattatg tatactaata tctagaggtt atgatatata tgtaatcttg attccaattt
     2461 tgactatttt agctagttta atcaatttaa tttgtgatat taaaaagcat aaatgattct
     2521 atattaatgc aactttgtat tcgtacaagg ttgtattttt ttcgcaaaca attacttaat
     2581 gacagcgcat caataaatct aaccaacaca ctatcgcaaa catttatgga tattttgtga
     2641 ttttataaaa gcaaacgtag aataatgcga taagtaataa tgctatcgtt aaaaataaga
     2701 atgacaatgc aataaaatca tattcaaagc atagaattga agacattaaa aatactgcac
     2761 tcaaactgat acatagcgta aatatatatg caatttggtt cttgtgttta tctgcaatgg
     2821 tcgtttgatt tatataagaa attagtgtga aatctttagt tattgcgaca taaatgccta
     2881 atataaccat acttatgcag aatacgatac taaatacgat gttgacggtc attgttgcac
     2941 cttcctgata gaatataatg tactgtcttt tagtaatgaa tgcgtatctt atatatgtat
     3001 gtttctaaaa aacagtacgt ttttatcaat ttttaattta ttagttgaat gaatggggtt
     3061 atgaaaaagg aagttttaat cttttgtcat gaaataaatg ggatgaatat cacgactaga
     3121 agtaatgtta cgaacaggag cgtataaatc tagagacgct aaattcgaca tagtatgttg
     3181 ctcaattatg gctgatgatg agtttaaagt atgtgcgttg gaactgtcgg atttttgttc
     3241 ataatgtttt gcatattgtg ccatgatgaa tagtgtaaaa ataaataaaa caataagaga
     3301 tataatgccc ataatcaaaa gtatttgttt agagcctttc attatttcac atcctttcta
     3361 aaatatattt gtaactaaat ttaaaatagt tatttttgta attctaaacc tttttcatcg
     3421 cgaaaacaat taaataggtc gcggtattaa ttattatatt attaccgctt aatatgaaaa
     3481 atacatgaaa attaattttc taatatactt ttgaaaaatt attacaaatt agccccttca
     3541 aaacgcgaaa acataaggat tctagtttca aaagggctga taagtataaa atgaaatgta
     3601 atatttcgat gtataaaatt tttaattagc taaaaatcat cgcattaatt ttttgagcta
     3661 catcatcaaa atccggacat tttaacgaca catataattt aattttaggt tcagtaccag
     3721 aaggacgtaa agcgataaat ccttcgtcaa ataagacacg aataacattt gatttagaag
     3781 aattaatctg tgacgttgta tctttatcca aatgataaac ctcgctagtt aaataatctt
     3841 caattgcttt cactttcagt ccttgaatct cttgcggtgg atttgaacgg aaatgtgtca
     3901 taattgattc aatttttttc ttaccttcta atccttctag cgtatgcgag aataatgtat
     3961 cctcatgtct accaacagtt tgataaatct gttctaattc atctttcaat gttttgccat
     4021 ataattttaa ctcagaagcg tattttataa tgagtggcac aatttgtacg gcatctttat
     4081 cacgtacaaa aggctctgat agaaaaccgt aactctcttc aaatgcgaaa atcatatttt
     4141 gatgatcatc cagttgtctt atttcctgag caataaattt aaagcccgtc agtacctctt
     4201 tgtatttaac attattatag cgtgctaatg actttgttaa ctcactactt acaatagatt
     4261 gaatcattaa gcggtgacgt aattgagacg tttgttgaat acgataatta agtaacagcg
     4321 caccaatttg gttgccatta aaatatgtga tatgaccatg tgcatcacgt tcagcaatac
     4381 caagtcgatc tgcatctggg tcagttgata ttaataagtc tgcatgactt ttatttgcaa
     4441 gttcgactgc ttgatcaaaa gcgcgatgat cttcaggatt agcactttga actgaactaa
     4501 aattcggatc aggtttacat tgtgcatcta caagattaaa ttgattgaaa ttcaaagatt
     4561 ttaataactc aggtacaatt ggaacacttg taccatgcaa actagtgaac acaacttgta
     4621 aatcagattt aggaatatag ccaatcatat tttgaatgtg tttcatatag tcatcagtta
     4681 cagatttcgg aaaaggtttg atataagatg tattttgttt tgaaataggt atatcaatct
     4741 gtaatggatc gccaacttct tcgatataac gacttgcaag ctcagatgca tcagtcgata
     4801 attgcgcgcc atcagaacca tatactttaa tgccgttata gtctttcgga ttatgacttg
     4861 ctgtaatcat aatgccggca gtagtattaa gattgcgaac cgcgaacgat aattccggtg
     4921 tcgttttata tgtatcaggt aaataaactg ttatttgatg gtttgctaat acattagcaa
     4981 taatttgggc gaattcagtt gaaagatgtc taatatcata atgaatgact attgttggac
     5041 tgtttgtttg ggcatttaaa taacgcgcta aacctaatgc caatttttca atagtaaact
     5101 tattaagtcg accttcacca agaccgaatt ttccgcgtat acccgcagta ccaaatgtta
     5161 atttactttc aaaaccttct cgctgttcaa tgtcagattg ctgctcataa aaatgtttaa
     5221 ctaaactatc attagctctt tctatccata attctttatc cattgttgct aaacatcctt
     5281 tcaaaatctc agttagactt aataaaacat gaaaactaaa gcccttacat ttatgtaatg
     5341 aattataaag aaatacgccc caaaagtaaa aaaacacagc cccaagacaa tacttttcac
     5401 aagtattata taatagatgt gtatgaaaat gcatgga
//
LOCUS       DAHHSC010000162         5395 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.163, whole genome shotgun sequence.
ACCESSION   DAHHSC010000162 DAHHSC010000000
VERSION     DAHHSC010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 5395)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5395)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5395
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.163"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            16..963
                     /locus_tag="NBI57_002109"
     CDS             16..963
                     /locus_tag="NBI57_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002449086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCD1823040.1"
                     /translation="MLLLLLILLTIISLFIGVSELSIKDLLHLTESQRNILFSSRIPR
                     TMSILIAGSSLALAGLIMQQMMQNKFVSPTTAGTMEWAKLGILIALLFFPTGHILLKL
                     VFAVICSICGTFLFVKIIDFIKVKDVIFVPLLGIMMGGIVASFTTFISLRTNAVQSIG
                     NWLNGNFAIITSGRYEILYLSIPLLALTYLFANHFTIVGMGKDFTNNLGLSYEKLINI
                     ALFITATITALVVVTVGTLPFLGLVIPNIISIYRGDHLKNAIPHTMMLGAIFVLFSDI
                     VGRIVVYPYEINIGLTIGVFGTIIFLILLMKGRKNYAQQ"
     gene            950..1906
                     /locus_tag="NBI57_002110"
     CDS             950..1906
                     /locus_tag="NBI57_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HCD1823041.1"
                     /translation="MRNNNKKIMLLIAVTLLISMLYLFVGIDFEIFEYQFSSRLRKFI
                     LIILVGAAIATSVVIFQAITNNRLLTPSIMGLDAVYLFIKVLPVFLFGIQSVWVTNVY
                     LNFILTLITMVLFALILFQGIFKIGHFSIYFILLIGVLLGTFFRSITGFIQLIMDPES
                     FLAIQSSMFANFNASNSNLVTFSAVLLVILLVITILLLPYLDVLLLGRAEAINLGISY
                     EKLTRILLVIVSVLVSVSTALVGPITFLGLLTVNLAHELMKTYEHKYILIATICLSWI
                     SLFSAQWVVENVFEATTEMSILIDLIGGSYFIYLLVRRRNAQ"
     gene            1903..2664
                     /locus_tag="NBI57_002111"
     CDS             1903..2664
                     /locus_tag="NBI57_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCD1823042.1"
                     /translation="MIQVENLTKTINNQMILEDISIDIEKGKLTSLIGPNGAGKSTLL
                     SAICRLIRFDNGEVKIDGQLMSDYKNNDLSKKISILKQTNHTEMNITVEQLVNFGRFP
                     YSKGRLTKEDHDIVNDALDLLQLQDIRNRNIKSLSGGQRQRAYIAMTIAQDTEYILLD
                     EPLNNLDMKHAVQIMQTLKMLAHKMNKAIVIVLHDINFASCYSDQIVALKNGQLVKSD
                     LKDNVIQSSVLSDLYDMNIQIEHIRNQRICLYFKD"
     gene            2783..3811
                     /locus_tag="NBI57_002112"
     CDS             2783..3811
                     /locus_tag="NBI57_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HCD1823043.1"
                     /translation="MKKTVLYLVLAVMFLLAACGNNSDKEQSKSETKGSKDTVKIENN
                     YKMRGEKKDGSDAKKVKETVEVPKNPKNAVVLDYGALDVMKEMGLSDKVKALPKGEGG
                     KSLPNFLESFKDDKYTNVGNLKEVNFDKIAATKPEVIFISGRTANQKNLDEFKKAAPK
                     AKIVYVGADEKNLIGSMKQNTENIGKIYDKEDKAKELNKDLDNKIASMKDKTKNFNKT
                     VMYLLVNEGELSTFGPKGRFGGLVYDTLGFNAVDKKVSNSNHGQNVSNEYVNKENPDV
                     ILAMDRGQAISGKSTAKQALNNPVLKNVKAIKEDKVYNLDPKLWYFAAGSTTTTIKQI
                     EELDKVVK"
     gene            complement(4128..4442)
                     /locus_tag="NBI57_002113"
     CDS             complement(4128..4442)
                     /locus_tag="NBI57_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1823044.1"
                     /translation="MPKVYGSLIDTETRCRHYFTEEDIIAIKFKCCNKYYPCYKCHNE
                     FEKHAIKRWSEPSFNEKAILCGVCKHELTINEYMMVERCPNCQSRFNNRCKYHYHIYF
                     EI"
     gene            complement(4460..5383)
                     /locus_tag="NBI57_002114"
     CDS             complement(4460..5383)
                     /locus_tag="NBI57_002114"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HCD1823045.1"
                     /translation="MINKDIYQALQQLIPNEKIKVDEPLKRYTYTKTGGNADFYITPT
                     KNEEVQAVVKYAYQNEIPVTYLGNGSNIIIREGGIRGIVISLLSLDHIDVSDDAIIAG
                     SGAAIIDVSRVARDYALTGLEFACGIPGSIGGAVYMNAGAYGGEVKDCIDYALCVNEQ
                     GSLIKLTTKELELDYRNSIIQKEHLVVLEAAFTLAPGKMTEIQAKMDDLTERRESKQP
                     LEYPSCGSVFQRPPGHFAGKLIQDSNLQGHRIGGVEVSTKHAGFMVNVDNGTATDYEN
                     LIHYVQKTVKEKFGIELNREVRIIGEHPKES"
BASE COUNT         1845 a          769 c          950 g         1831 t
ORIGIN      
        1 ttattaaagg gaaatatttt gcttctatta ctaatattgt tgacaattat ttcgttgttc
       61 ataggtgtga gtgaactatc aattaaagat ttactacatt taactgaatc acagcggaat
      121 attttattct caagccgaat accaaggacg atgagtattt taattgctgg aagttcgttg
      181 gctttagcag gcttgataat gcaacaaatg atgcaaaata agtttgttag tccgactaca
      241 gctggaacga tggaatgggc taaactaggt attttaattg ctttattgtt ctttccaacc
      301 ggtcatattt tattaaaact agtatttgct gttatttgca gtatttgcgg tacgttttta
      361 tttgttaaaa tcattgattt tataaaagtg aaagatgtca tttttgtacc gcttttagga
      421 attatgatgg gtgggattgt tgcaagtttc acaaccttca tctcattgcg cacgaatgct
      481 gttcaaagca ttggtaactg gcttaacggg aactttgcca ttatcacaag tggacgctat
      541 gaaattttat atttaagtat tcctctttta gcattgacat atctttttgc taatcatttc
      601 acgattgtag gaatgggtaa agactttact aataatttag gtttgagtta cgaaaaatta
      661 attaacatcg cattgtttat tactgcaact attacagcat tggtagtggt gactgttgga
      721 acattaccgt tcttaggact agtaatacca aatattattt caatttatcg aggtgatcat
      781 ttgaaaaatg ctatccctca tacgatgatg ttaggtgcca tctttgtatt attttctgat
      841 atagttggca gaattgttgt ttatccatat gaaataaata ttggtttaac aataggtgta
      901 tttggaacaa tcattttcct tatcttgctt atgaaaggta ggaaaaatta tgcgcaacaa
      961 taataaaaaa ataatgcttt taattgcagt aacgttatta attagtatgc tgtacttatt
     1021 tgtaggtatt gattttgaaa tatttgaata tcaattttca agtcgtttaa gaaagttcat
     1081 attaattatt ttagtaggtg ctgccattgc aacttcagtg gtgatttttc aagcgattac
     1141 aaataaccgt ctattgacac catcaataat ggggttagat gcagtttatt tatttatcaa
     1201 agtattgcca gtctttttat ttggaattca atcggtatgg gttactaatg tatatttgaa
     1261 ctttatatta acacttataa cgatggtgtt attcgcacta atcctattcc aaggtatctt
     1321 taaaattgga catttttcaa tttattttat cttacttatt ggtgtccttt taggaacatt
     1381 ttttagaagc ataacaggtt ttattcaact gattatggat cctgagtcat ttttagcaat
     1441 acaaagtagt atgtttgcta attttaatgc ttctaattcg aatttagtta ctttctcagc
     1501 agtgctatta gtaatcttat tagtcattac aattttacta ttgccttatt tagatgtatt
     1561 gcttttaggt cgtgctgaag caattaatct tgggatatcg tatgaaaaat taacgcgaat
     1621 tctacttgta atagtctcag ttttagtttc tgtgtcaact gcattagtag gaccaattac
     1681 atttttaggt ttattaactg taaatctagc gcatgaacta atgaagacgt atgaacataa
     1741 gtatatttta attgcgacaa tttgcttgag ttggattagt ttatttagtg cgcaatgggt
     1801 agttgaaaat gtgtttgaag ctacgacaga aatgagtata cttattgatt tgattggtgg
     1861 aagttatttc atttatctat tagttagaag gagaaatgcg caatgattca agttgaaaat
     1921 ttaactaaaa ctataaataa tcaaatgata ttggaagata ttagcataga tatcgaaaaa
     1981 ggtaaattga cttctttaat tggacctaat ggtgcgggta agagtacttt actttcagcg
     2041 atatgtaggt taattcgttt tgataacggt gaagtgaaaa tagatggaca gctcatgtct
     2101 gattataaaa ataatgactt gtcgaaaaaa atatctatat taaaacaaac aaaccatact
     2161 gaaatgaata ttacggtaga gcagttggta aactttggac gattccctta ttctaaaggt
     2221 cgtttgacga aagaggatca tgatattgtc aatgatgcgc tagatttgtt gcaactacaa
     2281 gatatcagaa atcgtaatat taagtcatta tctggtggac aacgtcagcg tgcatacatt
     2341 gcaatgacaa tagcacaaga tactgaatat attttgctag atgaaccatt aaataattta
     2401 gatatgaagc atgctgttca aattatgcaa acgttaaaaa tgttagcgca taaaatgaat
     2461 aaagcgattg tcattgtgtt acatgatatt aactttgcgt cctgttattc agatcagatt
     2521 gtagcattga aaaacggaca actagttaag tcagatttga aagataatgt cattcaaagt
     2581 agtgttttaa gtgatttata tgacatgaat attcaaattg aacatataag aaatcaaagg
     2641 atttgtttat attttaagga ttgataattt ggagacactt taaaggggtg atgcgccaat
     2701 taaagaaggg ttaaacgtaa agcatttatt tatatttcac atcaagcaca cagattaagc
     2761 caaaagagga gaatattata ttatgaagaa aacagtctta tatttagtat tagcagtaat
     2821 gtttttatta gcggcatgcg gtaacaattc tgataaagaa caatcaaaat cagaaactaa
     2881 aggttctaaa gatacagtaa aaattgaaaa taactataaa atgcgtggcg agaaaaaaga
     2941 tggtagtgac gctaaaaaag ttaaagaaac tgttgaagta ccaaaaaatc ctaaaaatgc
     3001 agttgtgtta gactatggcg cattagatgt aatgaaagaa atgggcttat cagataaagt
     3061 aaaagcatta cctaaagggg aaggcggtaa gtcattaccg aatttcttag aatcatttaa
     3121 agatgataaa tatacaaacg ttggtaattt aaaagaagtg aattttgata aaattgctgc
     3181 gacgaaaccc gaagtaatct ttatctctgg acgtacagct aatcaaaaga atttagatga
     3241 attcaaaaaa gctgcaccta aagcgaaaat tgtttatgtt ggtgcagatg aaaagaactt
     3301 aattggttca atgaaacaaa acactgaaaa tatcggtaaa atttacgata aagaagataa
     3361 agctaaagaa ttaaataaag atttagataa caaaattgct tcaatgaaag ataaaacgaa
     3421 aaacttcaat aaaactgtta tgtatttact agttaacgaa ggtgaattat caacatttgg
     3481 acctaaaggt cgttttggtg gattagttta cgatacatta ggattcaatg cagttgataa
     3541 aaaagtaagt aatagcaatc atggacaaaa tgtttctaac gaatatgtta ataaagaaaa
     3601 tccagatgtt attttagcga tggatagagg tcaagcgata agtggtaaat caactgcgaa
     3661 acaagcatta aataatcctg tattaaaaaa tgttaaagca attaaagaag acaaagtata
     3721 taatttagat cctaaattat ggtactttgc agctggatca actacaacta caattaaaca
     3781 aattgaggaa cttgataaag ttgtaaaata attttaaaag aggggaacaa tggttaaagg
     3841 tcttaatcat tgctcccctc ttttctttaa aaaaggaaat ctgggacgtc aatcaatgtc
     3901 ctagactcta aaatgttctg ttgtcagtcg ttggttgaat gaacatgtac ttgtaacaag
     3961 ttcatttcaa taccagtggg ctccaaacat agagaaattt gattttcaat ttctactgac
     4021 aatgcaagtt ggcggggccc aaacatagag aatttcaaaa aggaattcta cagaagtggt
     4081 gctttatcat gtctgaccca ctccctataa tgttttgact atgttgttta aatttcaaaa
     4141 taaatatgat agtgatattt acagcgattg ttaaaccgag attggcaatt tggacaacgc
     4201 tctaccatca tatattcatt gattgttaat tcgtgtttgc atacaccgca taagattgct
     4261 ttttcgttaa atgaaggctc agaccaacgc ttaatggcgt gcttttcaaa ctcattatgg
     4321 cacttatagc atggatagta tttattacaa catttaaatt taatagcaat aatatcttct
     4381 tcggtaaaat aatggcgaca gcgtgtttca gtatcgatta atgaaccata aactttaggc
     4441 atagacaaag ctccttaact tacgattcct ttggatgttc accaataatg cgaacttcac
     4501 gatttaattc aatgccaaat ttttctttga cggtcttttg tacataatga ataaggtttt
     4561 cataatctgt agcagttcca ttgtctacat ttaccataaa accagcgtgt ttggttgaaa
     4621 cttcaacgcc gccaatacgg tgaccttgca aattagaatc ttgtatcaat ttacctgcaa
     4681 aatgaccagg cggtctttgg aatacactac cacatgaagg atactctaaa ggttgtttag
     4741 attctctacg ttctgttaaa tcatccattt tagcttgtat ttcagtcatt ttaccaggag
     4801 ctaaagtaaa tgcagcttct aatacaacta agtgttcttt ttgaataatg ctattacgat
     4861 aatctaactc taattctttt gttgtaagtt taattaacga gccttgttcg tttacgcaaa
     4921 gcgcatagtc tatacaatct ttaacttcgc caccataagc gccagcattc atatacactg
     4981 caccaccaat tgaacctgga ataccacatg caaattcaag gccagtaagt gcgtaatcac
     5041 gagcaacacg tgagacatca ataattgcag cgccgctacc ggctattatc gcatcatcag
     5101 atacatcgat atgatctagt gataataaac taattacaat gccgcgaata ccaccttcac
     5161 ggataataat atttgagcca tttcctaaat atgtaacagg aatctcattt tgataggcat
     5221 atttaacaac tgcttgtact tcttcatttt tagtaggggt aatataaaag tcggcattac
     5281 cacctgtttt agtataagtg tatcgtttta aaggttcatc aactttaatt ttttcatttg
     5341 ggataagttg ttgtaaagct tgatagatgt ctttatttat cacttctcag tacat
//
LOCUS       DAHHSC010000163         5361 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.164, whole genome shotgun sequence.
ACCESSION   DAHHSC010000163 DAHHSC010000000
VERSION     DAHHSC010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 5361)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5361)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5361
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.164"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            18..578
                     /locus_tag="NBI57_002115"
     CDS             18..578
                     /locus_tag="NBI57_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCD1823046.1"
                     /translation="MNKVEAIKFNDDIVKMYEALKIKSERDYLFFKLAIHSGLKVSEL
                     LTITVSQVKRLIEKCTLSEMCKAHFHSLIKIRLPETLSKELLQYIEDRSLSNEDVLFQ
                     SLRTNQVLSRQQAYRIIHQASIEAGIDNVGLTTLRKTFAYHAYQKGIPIPVIQKYLGH
                     QSAIETLNFIGLENECEHSIYISLQL"
     gene            597..2999
                     /gene="mnhA2"
                     /locus_tag="NBI57_002116"
     CDS             597..2999
                     /gene="mnhA2"
                     /locus_tag="NBI57_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh2 subunit A"
                     /protein_id="HCD1823047.1"
                     /translation="MSLVYLLIAILVIMAMILLMSKRRALAKYAGYIALVAPVISSIY
                     FLIQIPSVAKLQYLSTSIPWIKTLDINLDLRLDGLSLMFSLIISLIGIAVFFYATQYL
                     SSRKDNLPRFYFYLTLFMFSMIGIVLSDNTILMYIFWELTSVSSFLLISYWYNNGDSQ
                     FGAIQSFMITVFGGLALLVGFIMLYIMTGTNNITDILGQADHIKNHGLFIPMIFMFLL
                     GAFTKSAQFPFHIWLPRAMAAPTPVSAYLHSATMVKAGIFLLLRFTPLLGLSNMYIYI
                     VTFVGLITMLFGSITALKQWDLKGILAYSTISQLGMIMAMVGIGGGYAQHQQDAIASI
                     YVFVLFGALFHLMNHAIFKCALFMGVGILDHEAGSRDIRILSGMRQLFPKMNLVMTIA
                     ALSMAGVPFLNGFLSKEMFLDALTQTGQLSQFSLISMIAIVFVGVIASIFTFTYALYM
                     VKEVFWTKYDSKVFTKKNIHEPWLFSLPSLILMVLVPVIFFVPNIFGKGIIVPALRGV
                     SGGNHQIDPLAPHVSQWHGFNIPLLLTIIIILLGSVLAIKVDWKKVFTGKIRQISVSK
                     GYEMVYRHFEKFATKRFKRVMQDRLNQYIIMTLGIFMIIIGYGYIRIGLPKVHQLHVS
                     EFGALEIILAIVTVTIGISLIFIRQRLTMVILNGVIGFVVTLFFIAMKAPDLALTQLV
                     VETITTILFIVSFSRLPNVPRSNANKKREIIKISVSLLMALIVVSLIFITQQTDGLSS
                     ISDFYLKADKLTGGKNIVNAILGDFRALDTLFEGLVLIITGLGIYTLLNYQDRRGQDE
                     RE"
     gene            2986..3411
                     /gene="mnhB2"
                     /locus_tag="NBI57_002117"
     CDS             2986..3411
                     /gene="mnhB2"
                     /locus_tag="NBI57_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh2 subunit B"
                     /protein_id="HCD1823048.1"
                     /translation="MKENDVVLRTVTKLVVFILLTFGFYVFFAGHNNPGGGFIGGLIF
                     SSAFILMFLAFNVEEVLESLPIDFRILMIIGALVSSITAIIPMFFGKPFLSQYETTWI
                     LPILGQIHVSTITLFELGILFSVVGVIVTVMLSLSGGRS"
     gene            3408..3752
                     /gene="mnhC2"
                     /locus_tag="NBI57_002118"
     CDS             3408..3752
                     /gene="mnhC2"
                     /locus_tag="NBI57_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh2 subunit C"
                     /protein_id="HCD1823049.1"
                     /translation="MNLILLLVIGFLVFIGTYMILSINLIRIVIGISIYTHAGNLIIM
                     SMGTYGSSRSEPLITGGNQLFVDPLLQAIVLTAIVIGFGMTAFLLVLVYRTYKVTKED
                     EIEGLRGEDDAK"
     gene            3742..5238
                     /gene="mnhD2"
                     /locus_tag="NBI57_002119"
     CDS             3742..5238
                     /gene="mnhD2"
                     /locus_tag="NBI57_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh2 subunit D"
                     /protein_id="HCD1823050.1"
                     /translation="MLSNLLILPMLLPFLCALILVFLKNNDRISKYLYLGTMTITTII
                     SLMLLIYVQRHRPITLDFGGWSAPFGIQFLGDSLSLIMVTTASFVITLIMAYGFGRGE
                     HKANRYHLPSFILFLSVGVIGSFLTSDLFNLYVMFEIMLLASFVLITLGQSVEQLRAA
                     IIYVVLNIIGSWLFLLGIGLLYKTVGTLNFSHIAMRLNDMGDNRTVTMISLIFLVAFS
                     AKAALVLFMWLPKAYAVLNTELAALFAALMTKVGAYALIRFFTLLFDQHNDLIHPLLA
                     TMAAITMVIGAIGVIAYKDIKKIAAYQVIISIGFIILGLGTNTFAGINGAIFYLVNDI
                     VVKTLLFFIIGSLVYITGYRQYQYLNGLAKKEPLFGVAFIIMIFAIGGVPPFSGFPGK
                     VLIFQGALQNGNYIGLALMIITSLIAMYSLFRILFYMYFGDKDGEEVNFKKIPLYRKR
                     ILSILVVVVIAIGIAAPVVLNVTSDATELNTSDQLYQKLVNPHLKGED"
     gene            5239..>5361
                     /gene="mnhE2"
                     /locus_tag="NBI57_002120"
     CDS             5239..>5361
                     /gene="mnhE2"
                     /locus_tag="NBI57_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh2 subunit E"
                     /protein_id="HCD1823051.1"
                     /translation="MNQIVLNIIIAFLWVLFQDEDHFKFSTFFSGYLIGLIVIYI"
BASE COUNT         1668 a          752 c          965 g         1976 t
ORIGIN      
        1 caaggggagg ttgagtaatg aataaagtag aagcgattaa atttaatgat gatattgtta
       61 aaatgtatga agcgctcaag ataaaatctg aacgtgacta tttattcttt aagttagcta
      121 tacatagtgg attgaaagta tcagaattat taacaattac agtctctcaa gttaagagac
      181 taattgaaaa gtgtacgtta tcagaaatgt gtaaagcaca ttttcattcg ttgattaaaa
      241 ttaggttacc agaaacatta tcgaaagaac tacttcaata tatagaggac aggagtcttt
      301 cgaatgaaga cgttcttttt caatcactac gaacaaatca agtattatct agacagcaag
      361 catatcgaat aattcaccaa gcatcaattg aagctggtat agataatgta ggactaacga
      421 cattgcgtaa gacatttgca tatcatgctt atcaaaaagg tatacctata ccagtcattc
      481 aaaagtattt agggcatcaa tctgctattg aaacactaaa ttttatcggt ttagaaaatg
      541 agtgtgaaca tagtatttat atttcattac aattatagaa acaaaggagg ctaataatga
      601 gtttggttta tttattaatt gctatacttg tgattatggc gatgatactt ctaatgtcta
      661 aacgtagagc attggctaaa tatgccgggt acatagcgtt ggttgcacct gtaatttcat
      721 ctatctattt tttgattcaa ataccatcag tagctaaact gcaatatctt tctacctcta
      781 ttccatggat taagacatta gatattaatt tagatttacg tttagatggt ttaagtttaa
      841 tgttttctct tattatttca cttattggaa ttgcagtatt cttctatgca actcaatatt
      901 tatcctctcg aaaagacaat ttaccaaggt tttattttta tttaacgtta tttatgttca
      961 gtatgattgg tattgtatta tcagacaata cgatattgat gtacattttt tgggaattaa
     1021 cgagtgtatc atcattttta ttgatttcat attggtataa caatggtgac agtcaatttg
     1081 gtgcgattca atcatttatg attacagtat ttggtggatt ggcgttatta gttggtttta
     1141 ttatgctgta tatcatgaca ggaacgaata atatcactga catattagga caagcagatc
     1201 atattaagaa tcatggattg tttatcccta tgatttttat gtttttatta ggtgcattta
     1261 caaaatcagc acaatttcca tttcatattt ggctacctag agcaatggct gcacctacac
     1321 ctgtaagtgc ttatttacat tcagccacga tggtaaaagc tggtatcttt ttattacttc
     1381 gatttacacc attattaggt cttagcaata tgtacatata tatcgtcacg tttgttggtt
     1441 taataacaat gttatttggt tcaatcacag ctttaaaaca atgggattta aaaggaatcc
     1501 tagcatattc aacaatcagt caactaggaa tgattatggc aatggtcggt atcggtggtg
     1561 gatatgctca acaccaacaa gacgcaatag catctattta tgtatttgta ttatttggtg
     1621 cgctatttca tctaatgaat catgccatct ttaaatgtgc gcttttcatg ggagtaggta
     1681 ttttagatca tgaagcaggt tcaagggata tacgaatttt aagtggaatg cgtcaactat
     1741 ttcctaaaat gaatctagtc atgacgatag cggctctatc tatggctgga gtaccatttt
     1801 taaatggatt tttaagtaaa gaaatgtttt tagatgcatt aacacaaact ggacaattat
     1861 cccaatttag tttgatttca atgatagcta tcgtgtttgt tggtgttatt gctagtattt
     1921 ttacattcac atatgcactt tacatggtta aagaagtatt ttggactaaa tatgattcta
     1981 aagtgtttac taaaaaaaat atccacgaac catggttgtt tagtttaccc tctcttatat
     2041 taatggtgct agtacctgta atcttttttg tgccaaatat atttgggaag gggattatcg
     2101 ttccagcatt aagaggtgta tcaggtggta atcatcaaat tgatccattg gcaccacatg
     2161 tttcgcaatg gcatggattt aacataccgc ttcttttaac catcatcatt attttattgg
     2221 gtagtgtact agcaatcaaa gtagattgga aaaaagtgtt cacaggtaaa attagacaga
     2281 tttcagtttc aaaagggtat gagatggtat atcgacattt tgaaaagttt gctacgaagc
     2341 gatttaaacg tgttatgcaa gatcgtttaa accaatacat tattatgacc ttaggcatat
     2401 ttatgattat cattggatat ggttatattc gaattggact tcctaaagta catcagttac
     2461 atgtttctga atttggggca ttagaaatta tattagcaat cgtaactgtc acaattggta
     2521 tttctttaat ttttatacgt caacgactga caatggtcat tttaaatgga gtcatcggat
     2581 ttgttgtgac cttattcttt atagcaatga aagcccctga tttagcattg actcagctag
     2641 tagttgaaac aataacgacg atactattta ttgtcagttt ttcaagatta ccaaacgtac
     2701 caagatctaa cgctaacaaa aaaagagaaa taattaaaat ttctgtatca ctcttgatgg
     2761 cacttattgt tgtatcatta atttttatta cacaacaaac agatggttta tcatcaatat
     2821 cagactttta tttaaaagct gacaaactaa caggtggtaa aaatattgta aatgcgatac
     2881 ttggtgactt tagagcatta gatacattat ttgaaggatt agtgttaatt attactgggc
     2941 taggtattta cacattatta aattatcaag atcggagggg acaagatgaa agagaatgat
     3001 gtcgtgttaa gaacggtcac gaaacttgtt gtatttattt tattgacttt cggattctat
     3061 gtcttcttcg caggtcataa taatcctggt ggtggcttta ttggtggttt aatatttagt
     3121 tcagcgttta ttttaatgtt tctggctttt aatgttgaag aggttttaga aagtttaccg
     3181 attgatttta gaattttaat gattattgga gcattggtat catctattac tgcgataata
     3241 cctatgtttt ttggaaaacc atttttgtct caatatgaaa caacttggat acttccaatt
     3301 ttagggcaaa ttcatgtaag tacaataaca ctttttgaat taggtatttt attctcagtt
     3361 gttggtgtta ttgtcacagt gatgttgtcg cttagcggag gtcgatcatg aatttaatat
     3421 tattactagt tataggattt ttagtgttta taggaacata tatgatttta tcaatcaatt
     3481 taattcgtat tgtaatcgga atttcaatat atactcatgc tggtaatctc attattatga
     3541 gtatgggaac gtatggttct agtagatcag aaccattaat aactggtgga aaccaattgt
     3601 ttgttgatcc cttgttacaa gctattgtac taactgcaat agttataggg tttgggatga
     3661 ctgcgttttt acttgtactt gtttatagaa cttataaagt aacaaaagaa gatgaaattg
     3721 aaggcctaag gggggaagat gatgctaagt aacttattga ttttaccaat gttattacca
     3781 ttcctttgtg ccttaatcct tgtattttta aaaaataatg atcgtatttc taaatattta
     3841 tacttaggta caatgactat caccacaatt atttcattaa tgctattaat ttatgttcag
     3901 cgtcaccgtc caattacgct agactttgga ggatggtcag cgccctttgg tatacagttt
     3961 ttaggagatt ctttaagttt gattatggtt acaaccgctt cgtttgtgat tactttaatt
     4021 atggcatacg gatttgggcg tggcgaacat aaagcaaatc gttatcactt gccatcgttc
     4081 atattatttt taagtgttgg cgtgataggc tcttttctaa catcagattt atttaattta
     4141 tacgtcatgt ttgaaattat gttactagcg tcatttgtac tcattacact tggacaatct
     4201 gtagaacaat tacgtgctgc aattatttat gttgtcttga atattattgg ttcatggcta
     4261 ttcttattag gtataggttt actttataaa acagtaggta cattaaactt ttcacatatt
     4321 gcaatgcgtt tgaatgacat gggagataat cgcactgtta caatgatttc attaatcttc
     4381 ttagtcgcat ttagtgcgaa agcagcgctg gtccttttta tgtggctacc caaagcctac
     4441 gctgtgttaa atactgagct tgcagcatta tttgcagcgt taatgaccaa agtaggggcc
     4501 tatgcattaa ttcgattctt cactttacta tttgatcaac ataatgatct catacatcca
     4561 ttgctagcaa ctatggctgc tataactatg gtcatcggcg ctataggtgt cattgcttat
     4621 aaagatatta aaaagattgc agcttaccaa gtcataatct caataggatt tatcatttta
     4681 ggtttaggaa caaacacgtt tgcaggtatt aatggtgcaa tattttattt ggtaaatgac
     4741 attgttgtaa aaacattgct attctttatt attggtagtt tagtttacat tacaggctat
     4801 cgacaatatc aatatttgaa tggcttagct aaaaaagaac ctttatttgg agttgcgttt
     4861 attataatga tttttgctat tggcggcgtg cctccattta gtggctttcc ggggaaagta
     4921 cttattttcc aaggtgcatt gcaaaatggc aattatattg gactagcgtt aatgattatt
     4981 actagtctaa ttgcaatgta cagtttattt aggatacttt tttatatgta ttttggagat
     5041 aaagatgggg aggaagttaa ttttaagaaa atcccgctat atcgaaaaag aattttaagt
     5101 attttagtag ttgtggttat cgcaatcgga attgctgcac ctgttgtgtt aaatgttaca
     5161 agtgatgcaa ctgagttgaa cacgagtgat caattatatc aaaaacttgt aaatccgcat
     5221 ttgaaaggag aggactaaat gaatcaaata gttttaaata ttatcattgc attcttatgg
     5281 gtattatttc aagatgaaga tcattttaaa ttctcgactt tcttttctgg atatctaatt
     5341 ggtttaattg tcatttatat a
//
LOCUS       DAHHSC010000164         5314 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.165, whole genome shotgun sequence.
ACCESSION   DAHHSC010000164 DAHHSC010000000
VERSION     DAHHSC010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 5314)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5314)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5314
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.165"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            60..1178
                     /gene="ald"
                     /locus_tag="NBI57_002121"
     CDS             60..1178
                     /gene="ald"
                     /locus_tag="NBI57_002121"
                     /EC_number="1.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HCD1823052.1"
                     /translation="MLVAVVKELKQGEGRVACTPENVRKLTDAGHKVIVEKNAGIGSG
                     FSNDMYEKEGAKIVTHEQAWEADLVIKVKEPHESEYQYFKKNQIIWGFLHLASSKEIV
                     EKMQEVGVTAISGETIIKNGKAELLAPMSAIAGQRSAIMGAYYSEAQHGGQGTLVTGV
                     HENVDIPGSTYVIFGGGVAATNAANVALGLNAKVIIIELNDDRIKYLEDMYAEKDVTV
                     VKSTPENLAEQIKKADVFISTILIPGAKPPKLVTREMVKSMKKGSVLIDIAIDQGGTI
                     ETIRPTTISDPVYEEEGVIHYGVPNQPGAVPRTSTMALAQGNIDYILEICDKGLEQAI
                     KDNEALSTGVNIYQGQVTNQGLASSHDLDYKEILNVIE"
     gene            1273..2313
                     /gene="tdcB"
                     /locus_tag="NBI57_002122"
     CDS             1273..2313
                     /gene="tdcB"
                     /locus_tag="NBI57_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional threonine ammonia-lyase/L-serine
                     ammonia-lyase TdcB"
                     /protein_id="HCD1823053.1"
                     /translation="MTTNTVTLQTAHIVSLGDIEEAKASIKPFIRRTPLIKSMYLSQN
                     ITKGNVYLKLENMQFTGSFKFRGASNKINHLSDEQKAKGIIGASAGNHAQGVALTAKL
                     LGIDATIVMPETAPIAKQNATKGYGAKVILKGKNFNETRLYMEELAKENGMTIVHPYD
                     DKFVMAGQGTIGLEILDDIWNVNTVIVPVGGGGLIAGIATALKSFNPSIHIIGVQAEN
                     VHGMAESFYKRALTEHREDSTIADGCDVKVPGEKTYEVVKHLVDEFILVSEEEIEHAM
                     QDLMQRAKIITEGAGALPTAAILSGKIDKKWLEGKNVVALVSGGNVDLTRVSGVIEHG
                     LNIADTSKGVVG"
     gene            2344..3666
                     /locus_tag="NBI57_002123"
     CDS             2344..3666
                     /locus_tag="NBI57_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCD1823054.1"
                     /translation="MSNGKELQKNIGFFSAFAIVMGTVIGSGVFFKISNVTEVTGTAG
                     MALFVWFLGGIITICAGLTAAELAAAIPETGGLTKYIEYTYGDFWGFLSGWAQSFIYF
                     PANVAALSIVFATQLINLFHLSIGSLIPIAIASALSIVLINFLGSKAGGILQSVTLVI
                     KLIPIIVIVIFGIFQSGDITFSLIPTTGNSGNGFFTAIGSGLLATMFAYDGWIHVGNV
                     AGELKNPKRDLPLAISVGIGCIMAVYLLINATFLLTLPIELLAGNLNAASDTSKILFG
                     ENGGKIITIGILISVYGTINGYTMTGMRVPYAMAERKLLPFSHLFAKLTKSGAPWFGA
                     IIQLIIAIIMMSMGAFDTITNMLIFVIWLFYCMSFVAVIILRKREPNMERPYKVPLYP
                     IIPLIAILAGSFVLINTLFTQFILAIIGILITALGIPVYYYKKKQKAA"
     gene            3822..5213
                     /gene="norB"
                     /locus_tag="NBI57_002124"
     CDS             3822..5213
                     /gene="norB"
                     /locus_tag="NBI57_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000414682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter NorB"
                     /protein_id="HCD1823055.1"
                     /translation="MEKPSREAFEGNNKLLIGIVLSVITFWLFAQSLVNVVPILEDSF
                     NTDIGTVNIAVSITALFSGMFVVGAGGLADKYGRIKLTNIGIILNILGSLLIIISNIP
                     LLLIIGRLIQGLSAACIMPATLSIIKSYYIGKDRQRALSYWSIGSWGGSGVCSFFGGA
                     VATLLGWRWIFILSIIISLIALFLIKGTPETKSKSISLNKFDIKGLVLLVIMLLTLNI
                     LITKGSELGVTSLLFITLLAIAIGSFSLFIVLEKRATNPLIDFKLFKNKAYTGATASN
                     FLLNGVAGTLIVANTFVQRGLGYSSLQAGSLSITYLVMVLIMIRVGEKLLQTLGCKKP
                     MLIGTGVLIVGECLISLTFLPEILYVICCIIGYLFFGLGLGIYATPSTDTAIANAPLE
                     KVGVAAGIYKMASALGGAFGVALSGAVYAIVSNMTNIYTGAMIALWLNAGMGILSFVI
                     ILLLVPKQNDTQL"
BASE COUNT         1839 a          761 c         1024 g         1690 t
ORIGIN      
        1 cataaccaaa ggataaaata attgtgaaat tattcacaaa tgaaagagga gtgttgcaaa
       61 tgttagttgc agtagtcaaa gaattaaaac aaggtgaagg acgtgtagct tgcacacccg
      121 aaaatgtgcg taagttaacg gatgcaggac ataaagtaat tgttgaaaaa aatgctggca
      181 ttggttcagg attttctaac gatatgtatg aaaaagaagg cgctaagatc gtaactcacg
      241 aacaagcatg ggaagctgat cttgttatca aagtaaaaga acctcatgaa agcgaatatc
      301 aatatttcaa aaagaatcaa attatctggg gatttttaca tctagcatct tcaaaagaaa
      361 tagtagaaaa aatgcaagaa gttggtgtaa ctgcgattag tggtgaaacc attataaaaa
      421 atggaaaagc agaattatta gcgccaatga gtgctatagc aggtcaacgc tcagcaatta
      481 tgggagctta ctactctgaa gcacaacatg gtggtcaagg tactttagtg actggtgtac
      541 atgaaaatgt ggatatacct ggtagtacat atgtgatttt cggtggtggc gtagcagcaa
      601 caaatgcagc aaatgttgcc ttgggactaa atgctaaagt aatcattatc gagttaaacg
      661 atgaccgcat taaatatctt gaagatatgt atgcagaaaa agatgtcaca gtagtcaaat
      721 caacaccaga aaatttagca gaacaaatta agaaagcaga tgtatttatt tctacaattt
      781 taattccagg tgcgaaaccg ccaaaattgg ttactcgtga gatggttaaa tcaatgaaaa
      841 aaggttcagt attaatcgat atagctattg accaaggtgg aactattgaa acaattagac
      901 caactacaat ttctgatcca gtgtatgaag aagaaggtgt gattcattat ggtgtaccaa
      961 atcaaccagg agcagtccca agaacttcaa caatggcatt agcacaagga aatattgatt
     1021 atatattaga aatttgtgac aaaggcttag aacaagcaat taaagataat gaagccttaa
     1081 gtactggtgt aaacatttac caaggacaag tgacaaatca aggattagct tcatcacatg
     1141 acctagatta taaagaaata ttaaatgtta tcgaatagat aataatttaa atgaaattga
     1201 gtgaaatgaa tattttaaat atagcattat agtttggact aaaaatttac aaaacggaag
     1261 gatgtaatta ttatgacaac caacacagtt acattacaaa cagcacatat tgtaagttta
     1321 ggagatatcg aagaagctaa agcaagcatt aaaccattta ttcgtcgaac accactaatt
     1381 aaatctatgt atttaagtca aaatatcact aaaggtaacg tatatttaaa acttgaaaat
     1441 atgcagttta caggttcatt taaatttaga ggtgctagca ataaaattaa tcatttgtca
     1501 gatgaacaaa aagctaaagg tattattggc gcatcagccg gaaatcatgc acaaggtgta
     1561 gctttgacag caaaactatt aggtattgat gcaacgattg tgatgccaga aacagcacca
     1621 attgctaaac agaatgctac caaaggatat ggtgcaaaag tcattttaaa aggtaaaaac
     1681 tttaatgaaa ctagacttta tatggaagaa ttagcgaaag aaaatggtat gacaattgtt
     1741 catccatatg atgataagtt tgtcatggct ggtcaaggaa caataggatt agaaatttta
     1801 gatgatatat ggaatgttaa tacggtcatt gttccagttg gtggaggtgg cctaattgca
     1861 ggtattgcca ccgctttaaa atcattcaac ccatcaattc atataatcgg cgttcaagct
     1921 gaaaatgtgc atggcatggc agagtcattc tataagagag ctttaactga acatcgtgaa
     1981 gatagcacaa ttgcagatgg ttgtgatgta aaagtaccag gtgaaaaaac atacgaagta
     2041 gtaaaacatt tagtagatga atttattctt gtatcagaag aggaaataga acatgcaatg
     2101 caagatttaa tgcaacgtgc caaaatcatt acagaaggtg caggagcttt acctacagca
     2161 gctattttaa gtgggaaaat tgataaaaaa tggctagagg gtaaaaatgt cgttgcatta
     2221 gtttcaggag gaaacgttga cttaactaga gtttctggtg tcattgaaca tggcttaaat
     2281 atcgcggata caagtaaggg tgtggtaggt taaaacttcc aatcttaaaa atgaggtgta
     2341 attatgtcaa atggtaaaga attacaaaaa aatataggtt tcttctcagc gtttgctatt
     2401 gttatgggga cagttattgg ttcaggagta ttctttaaaa tatcaaacgt aacagaagta
     2461 acaggaacag caggaatggc cttgtttgta tggttcctag gcggcatcat taccatttgt
     2521 gcggggttaa cagcagcaga acttgctgct gcaatccctg aaacaggtgg cttaacgaag
     2581 tatatagaat atacatacgg tgatttctgg ggcttcctat caggttgggc gcaatcattt
     2641 atttattttc cagctaacgt agcagcattg tctatcgtat ttgcgacaca gctaattaat
     2701 ttattccatt tatctatagg ttcgttaata ccaatagcaa tcgcatctgc gttatctatt
     2761 gtgttgataa atttcctagg ttcaaaagca ggcggaattt tacaatcagt tactttagta
     2821 attaaactga ttccaatcat cgttattgta atttttggta tttttcaatc tggagatatc
     2881 actttttcat taattccaac tacaggtaat tcaggaaatg gtttctttac agcaattggt
     2941 agtggtttat tagcaactat gtttgcatat gatggttgga ttcatgtagg aaatgttgcg
     3001 ggggaactta aaaatcctaa acgcgattta cctttagcga tttcagttgg tatcggttgt
     3061 attatggctg tgtatttatt aattaacgct acatttttat taacgttgcc aatagaacta
     3121 cttgctggta acttaaatgc agcttcagat acatcaaaaa tattatttgg tgaaaatggc
     3181 ggtaaaatta ttacaatcgg tatattaatt tctgtttatg gtacgatcaa tggctatact
     3241 atgactggta tgcgcgtacc atatgcaatg gctgaaagaa aattattgcc atttagccac
     3301 ttattcgcaa aattaacaaa atctggcgca ccatggtttg gcgcaattat acaacttata
     3361 atcgctatca tcatgatgtc aatgggagca tttgatacaa taacaaacat gttaatcttt
     3421 gttatttggt tgttctattg tatgtcattt gttgctgtaa taattttaag aaaacgtgaa
     3481 ccaaatatgg agcgtccata taaagtaccg ttatatccga tcataccttt aattgctatt
     3541 ttggcaggat catttgtatt gattaataca ctgtttacac aatttatatt agcaatcatt
     3601 ggaattctaa taacagcact tggtatacca gtttattact ataagaagaa acaaaaagca
     3661 gcataaggta agataactag cattgagaat aaatggatgg actactaata aatttaaagt
     3721 tttacacatt aaaatcaaaa accattcaat tattctatcg aacagacaaa tttctgttat
     3781 ggaatttgtc tgttttcaaa agtataggga ggcaaataga gatggaaaag ccgtcaagag
     3841 aggcatttga aggcaataat aagttgttaa taggaattgt tttaagtgta ataacgtttt
     3901 ggctatttgc acaatcattg gttaatgttg taccaatact tgaagatagt ttcaatacag
     3961 atattggaac ggttaatatc gccgttagta taactgcttt attttcagga atgtttgttg
     4021 taggagcagg tggtcttgct gataaatatg gcagaattaa actcacgaac attggtatta
     4081 tcttaaatat attgggttca ttattaatca ttatttcaaa tattccttta ttacttatta
     4141 taggaagatt aattcaagga ctttcagcag catgtattat gcctgcaact ttgtctatta
     4201 ttaagtcata ttacattggg aaagatagac aacgcgcttt aagttattgg tcaattggct
     4261 catggggcgg ctctggtgtt tgttcatttt ttggaggtgc agttgcaaca cttttaggtt
     4321 ggcgttggat tttcatccta tcaattataa tttcattaat tgcgctgttt cttattaaag
     4381 gcacacctga aactaaatct aaatcgattt ctctaaataa atttgacatt aaaggtctgg
     4441 ttcttttagt cattatgctc ctcactttaa atattttaat tactaaagga tcagaattag
     4501 gtgtaacctc acttcttttt atcactttat tagcaattgc aattggatct tttagtttat
     4561 ttatagttct tgaaaagcgt gctacaaatc ctttaatcga ttttaaatta tttaaaaata
     4621 aagcttatac aggtgcaaca gcttcaaact ttttgttaaa tggtgttgcg ggaacattaa
     4681 tagtagccaa cacatttgtt caaagaggtt taggatattc ctcattgcaa gcaggaagtt
     4741 tatcaatcac ttatttagta atggtactaa ttatgattcg tgttggtgaa aagttacttc
     4801 aaacactcgg atgcaagaaa ccaatgttaa ttggaacagg agttcttatt gtcggagaat
     4861 gtctcatttc attaactttc ttgccagaaa tattgtatgt catttgttgt attataggtt
     4921 atttattctt tggtttagga ctaggaatat atgctacacc atcaacagat acagcaattg
     4981 caaatgcacc gttagaaaaa gtaggagttg ctgcaggtat ctataaaatg gcttctgcat
     5041 taggtggagc atttggcgtc gcattgagtg gtgcagttta tgcaatcgta tcaaatatga
     5101 caaacattta tactggtgca atgattgcat tatggttaaa cgcaggtatg ggaatattat
     5161 cattcgttat cattttgtta cttgtgccta aacaaaacga cactcaatta tgataattga
     5221 gaattaaatt gaaatcatac aagtcgctac aatattaaac aaaaatataa accgattctt
     5281 atgtgtcatt attttaaatg aacataggga ttgg
//
LOCUS       DAHHSC010000165         5208 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.166, whole genome shotgun sequence.
ACCESSION   DAHHSC010000165 DAHHSC010000000
VERSION     DAHHSC010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 5208)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5208)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5208
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.166"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            complement(92..1909)
                     /locus_tag="NBI57_002125"
     CDS             complement(92..1909)
                     /locus_tag="NBI57_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HCD1823056.1"
                     /translation="MRIDQSIINEIKDKTDILDLVSEYVKLEKRGRNYIGLCPFHDEK
                     TPSFTVSEDKQICHCFGCKKGGNVFQFTQEIKDISFVEAVKELGDRVNVAVDIEATQS
                     NSNVQIASDDLQMIEMHELIQEFYYYALTKTVEGEQALTYLQERGFTDALIKERGIGF
                     APDSSHFCHDFLQKKGYDIELAYEAGLLSRNEENFSYYDRFRNRIMFPLKNAQGRIVG
                     YSGRTYTGQEPKYLNSPETPIFQKRKLLYNLDKARKSIRKLDEIVLLEGFMDVIKSDT
                     AGLKNVVATMGTQLSDEHITFIRKLTSNITLMFDGDFAGSEATLKTGQHLLQQGLNVF
                     VIQLPSGMDPDEYIGKYGNDAFTTFVKNDKKSFAHYKVSILKDEIAHNDLSYERYLKE
                     LSHDISLMKSSILQQKAINDVAPFFNVSPEQLANEIQFNQAPANYYPEDEYGGYDEYG
                     GYIEPEPIGMAQFDNLSRQEKAERAFLKHLMRDKDTFLNYYESVDKDNFTNQHFKYVF
                     EVLHDFYAENDQYNISDAVQYVNSNELRETLISLEQYNLNDEPYENEIDDYVNVINEK
                     GQETIESLNHKLREATRIGDVELQKYYLQQIVAKNKERM"
     gene            complement(1970..2788)
                     /locus_tag="NBI57_002126"
     CDS             complement(1970..2788)
                     /locus_tag="NBI57_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase/pyrophosphorylase"
                     /protein_id="HCD1823057.1"
                     /translation="MEKIKIIVASDSIGETAELVARAGISQFNPKQCKNELLRYPYIE
                     SFEDVDEVIQVAKDTNAIIVYTLIKPEMKQYMSEKVAEFQLKSVDIMGPLMDLLSASV
                     EEKPYNEPGIVHRLDDAYFKKIDAIEFAVKYDDGKDPKGLPKADIVLLGISRTSKTPL
                     SQYLAHKSYKVMNVPIVPEVTPPDGLYDIDPKKCIALKISEEKLNRIRKERLKQLGLG
                     DTARYATEARIQEELNYFEEIVSEIGCPVIDVSQKAIEETANDIIHYIEQNKSK"
     gene            complement(2799..3422)
                     /locus_tag="NBI57_002127"
     CDS             complement(2799..3422)
                     /locus_tag="NBI57_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001797828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCD1823058.1"
                     /translation="MELSQRQERIIEIVKTKGPITGEQIADKLNLTRATLRPDLAILT
                     MSGFLEARPRVGYYYSGKSKGKFFNEKLRQFEVKDYMSQPVVLRENTTVYDAICTIFL
                     EDVSTLFIINEDNDFVGVCSRKDLLRASMIGADIHTVPISVNMTRMPNVTYLEESELV
                     IYAADRMIEKEIDSIPIVRKKDNQKYEVIGRISKTTIAKLLVALYKE"
     gene            3757..5148
                     /locus_tag="NBI57_002128"
     CDS             3757..5148
                     /locus_tag="NBI57_002128"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HCD1823059.1"
                     /translation="MAKDMDTIVSLAKHRGFVFPGSDIYGGLSNTWDYGPLGVELKNN
                     VKKAWWQKFITQSPFNVGIDAAILMNPKVWEASGHLNNFNDPMIDNKDSKIRYRADKL
                     IEDYMQDVKGDENFIADGLSFEQMKKIIDDEGIVCPVSKTANWTEIRQFNLMFKTFQG
                     VTEDSTNEIFLRPETAQGIFVNYKNVQRSMRKKLPFGIGQIGKSFRNEITPGNFIFRT
                     REFEQMELEFFCKPGEEIEWQNYWKTFASDWLTSLNMSSENMRLRDHDEDELSHYSNA
                     TTDIEYKFPFGWGELWGIASRTDFDLRKHAEHSGEDFRYHDPETNEKYIPYCIEPSLG
                     ADRVTLAFLCDAYDEEGVEGSKDARTVLHFHPALAPYKAAILPLSKKLSGEAIKIFEQ
                     LSSKFSIDFDESQSIGKRYRRQDEIGTPYCVTFDFDSLEDNQVTVRDRDSMEQVRMPI
                     SELEAFLTEKTKF"
BASE COUNT         1664 a          913 c          770 g         1861 t
ORIGIN      
        1 tttcaatagt gttgtttaaa tgcggtaact atcatttacc cttatacatt ttgacataat
       61 cattattcgt attattcttt aaaatcacat gctacatgcg ttctttattc ttagcaacaa
      121 tttgctgtaa atagtatttt tgtaattcta catcgccaat ccttgtagct tcccttaatt
      181 tatgattcaa tgactcaatt gtttcttgtc ctttttcatt aataacattg acataatcat
      241 caatttcatt ttcatatggt tcgtcattca aattatattg ttctaagcta attagtgttt
      301 ctctcaactc atttgaatta acatactgca cagcatcact gatattatat tgatcatttt
      361 ccgcataaaa atcatgtaag acttcgaata catatttaaa atgctgattt gtgaagttat
      421 ccttatcaac actttcataa taatttaaaa atgtatcttt atctctcatt aaatgtttta
      481 aaaatgctcg ctccgctttt tcttgacggc tcaaattgtc aaattgtgcc ataccaattg
      541 gctcaggttc aatataaccg ccatactcat cataaccgcc atactcatct tctggataat
      601 aattggctgg tgcttgattg aattgtattt cgttagctaa ctgctcagga ctaacattga
      661 aaaatggcgc aacatcattt atagcctttt gttgcagaat tgatgacttc ataagtgaaa
      721 tgtcatgact cagttctttc aaataacgtt catatgaaag gtcattatgt gcaatttcat
      781 cttttaatat acttacttta taatgtgcaa atgacttttt gtcatttttt acaaaagtag
      841 taaatgcgtc gttgccatac ttaccaatgt attcatccgg atccatgcca gatggcaatt
      901 gtataacaaa tacatttagc ccttgctgta acaaatgttg acctgtttta agtgttgctt
      961 cactacccgc aaaatcccca tcaaacatta atgttatatt tgatgttaac tttcgtataa
     1021 aggtaatatg ttcatctgac aactgtgtac ccattgttgc aacaacgttt ttcaagccag
     1081 cagtatcaga ttttataaca tccataaaac cttctagtaa tacaatttca tctaattttc
     1141 taattgattt acgtgcttta tctaagttat ataacaactt tcttttttga aagataggcg
     1201 tttcaggact atttaggtat tttggttctt gaccggtata tgttcgacct gaatatccaa
     1261 caattcttcc ttgcgcattt ttcaaaggaa acataatacg atttcgaaat ctatcgtaat
     1321 aactgaaatt ttcttcgtta cgtgataata atccggcttc atatgctaat tcaatatcgt
     1381 aacccttttt ttgaagaaaa tcatgacaaa aatgtgagct atcgggtgca aagccaatgc
     1441 ctcgctcttt aataagcgca tctgtaaaac cacgttcttg taagtatgtt aatgcttgtt
     1501 cgccttcgac tgtctttgtt aaagcgtaat aataaaattc ttgtattaac tcatgcattt
     1561 caatcatttg taaatcatca gaagcaattt gaacatttga gttagattgt gttgcctcaa
     1621 tatctacagc aacattaact ctatcaccta attctttaac cgcttcaaca aatgatatgt
     1681 ctttaatttc ttgagtaaat tgaaaaacat tgccaccttt tttacaacca aaacaatgac
     1741 agatttgttt atcttcagaa actgtaaatg aaggtgtctt ttcatcatga aaaggacaca
     1801 aacctatata attgcgtcct ctcttttcta gttttacata ttcacttacc aagtctaaaa
     1861 tgtcggtttt atcttttatt tcattaatga tcgattgatc tattcgcaaa ttaaatcacc
     1921 tattaagcat tagttatact attataccta atttttgaca aaaatgaaat catttcgatt
     1981 tattttgttc aatataatgg attatatcgt ttgctgtttc ttcgattgct ttttgagaaa
     2041 catcaatgac aggacatcca atttcactta cgatttcttc aaagtaattc aattcttctt
     2101 ggattcgagc ttctgttgca taacgagctg tgtcacctag tcccaattgt tttaatcgct
     2161 cttttctaat gcgatttaat ttttcttcac ttattttaag tgcgatacat ttcttaggat
     2221 caatatcata taagccatct ggcggtgtca cttctggtac aatcggtaca ttcataactt
     2281 tgtaactctt atgcgctaaa tactgagata atggtgtctt tgaagttctc gaaataccaa
     2341 gtaaaacaat atcagcttta ggtaatcctt taggatcttt accatcatca tatttaaccg
     2401 caaactctat cgcatcaatt ttcttgaaat atgcatcatc taatctatga acgatacctg
     2461 gctcattata aggtttttct tcaaccgatg ctgataataa atccattaat ggccccatga
     2521 tatcaacgga cttcaattgg aattctgcta ctttctcact catatattgc ttcatttcag
     2581 gtttaataag tgtataaaca atgatagcat ttgtatcttt tgcaacttga atcacttcat
     2641 caacatcttc aaaagattca atatatggat atcttaataa ttcattttta cattgcttag
     2701 gattaaattg tgaaatacct gccctagcaa ctaactctgc cgtttcacct atagaatctg
     2761 aggctacgat aattttaatt ttttccatta cttctcacct attctttata taatgctact
     2821 aataacttag ctattgttgt tttggaaatt cttccaatta cttcatactt ttgattatct
     2881 ttttttctta caattggaat cgaatcaatt tctttttcaa tcattctatc tgctgcgtat
     2941 atgactaatt cgctttcctc taaataagtg acattaggca tacgtgtcat atttacactg
     3001 ataggtactg tatgaatatc tgctccaatc attgaagctc ttaataaatc ttttcttgaa
     3061 cacacaccaa caaaatcgtt atcttcatta ataataaata atgtacttac atcttctaaa
     3121 aaaattgtac aaatagcgtc ataaactgtt gtattctctc ttagcacaac aggttgagac
     3181 atatagtcct taacctcaaa ttgtcgaagt ttttcattaa aaaatttgcc ttttgattta
     3241 cctgaataat aatatccaac tcggggacgc gcttctaaaa aacctgacat tgttaatatc
     3301 gctaaatctg gtctaagcgt tgctcttgtt aaattcaact tatctgctat ttgttcacca
     3361 gtaatgggtc ctttagtttt aacaatttcg atgattcgtt cttgtctttg actgagttct
     3421 ataggtcttc accccttttt tattcactta ttattatacc gatatttctt tcatgtcaca
     3481 aacacattaa ttttacttgc ctttaaataa tctatcaatt agaataaacg tacagcgagt
     3541 taaggatagt gtaagcttaa caataagatt ggcgcaacga atcattttaa aataaaagcg
     3601 agtgactaca ctaatttggg tggaaccgcg ggttaaatac gtaacttatt ttgtattata
     3661 tacaatttaa aatattaact cgtcccatgg aagtaaagca tatttactat gctgttattt
     3721 tccatggggc gttttcatgt atgaggagag gtaattatgg caaaagatat ggatacaatt
     3781 gtttcattag caaaacacag aggttttgtg ttccctggta gtgatattta cggtggttta
     3841 tcaaacacat gggattatgg tcctttaggt gttgaattaa agaataatgt taaaaaagct
     3901 tggtggcaaa aattcattac acaatcaccg tttaacgttg gtatcgacgc tgcaatctta
     3961 atgaatccaa aagtatggga agcttcagga cacttaaaca acttcaacga cccaatgatt
     4021 gataataaag atagtaaaat tcgatatcgc gctgataaat taattgaaga ttatatgcaa
     4081 gatgttaaag gtgatgaaaa cttcattgcc gatggtttaa gttttgaaca aatgaaaaaa
     4141 attattgacg atgaaggtat tgtttgtcct gtaagtaaaa ctgctaactg gactgaaatt
     4201 cgccaattca atttaatgtt taaaacattc caaggtgtaa ctgaagattc tacaaatgaa
     4261 attttcttac gtcctgaaac agcacaaggt atttttgtaa actataaaaa cgtgcaacgt
     4321 tcaatgcgta aaaaattacc atttggtatc ggtcaaattg gtaaatcatt ccgtaatgaa
     4381 atcactccag gtaacttcat tttcagaaca agagaatttg aacaaatgga acttgaattc
     4441 ttctgtaaac ctggagaaga aatcgaatgg caaaattatt ggaaaacttt tgcaagtgac
     4501 tggttaacaa gcttaaatat gagcagtgaa aatatgcgtt tacgtgatca tgatgaagat
     4561 gaattatctc attactcaaa tgcaacaact gatattgaat ataaattccc atttggttgg
     4621 ggtgagttat ggggtatcgc aagtcgtaca gacttcgact tacgtaaaca tgctgaacac
     4681 tctggtgaag atttcagata ccatgatcca gaaacgaacg aaaaatatat tccatattgt
     4741 atcgagccat cacttggtgc agatcgtgta acattagctt tcttatgtga tgcatatgat
     4801 gaagaaggcg ttgaaggtag taaagatgca cgtacagttt tacacttcca tcctgcatta
     4861 gcaccatata aagcagcgat tttaccttta agtaagaaat tatctggcga agcgattaag
     4921 atttttgagc aattaagttc taaattctca atcgatttcg atgaatcaca atctatcggt
     4981 aaaagatacc gtcgtcaaga tgaaatcggt acaccttatt gtgtaacatt cgactttgat
     5041 tcattagaag ataatcaagt tacagtacgt gacagagatt caatggaaca agttcgtatg
     5101 ccaatctcag agttagaagc tttcttaact gaaaaaacaa aattctaatt aaaaagactt
     5161 tccaccatat gtggtggcta tataatttat aaaaagagat actggtta
//
LOCUS       DAHHSC010000166         5196 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.167, whole genome shotgun sequence.
ACCESSION   DAHHSC010000166 DAHHSC010000000
VERSION     DAHHSC010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 5196)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5196)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5196
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.167"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            18..704
                     /locus_tag="NBI57_002129"
     CDS             18..704
                     /locus_tag="NBI57_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain-containing protein"
                     /protein_id="HCD1823060.1"
                     /translation="MDKYQLKARPVVIRRELLDHYSDLGLDEQDLVILLKLIYASETS
                     NKQPSIELLQKGSTMQPRDITMVIQNLIQRELLELQVQKDEEGRFTEYMNLDPFFEKL
                     SHILKQQSMETKEQNSKEKFKQLFRVLEDTFARPLSPYEIETLNQWIDVDKHDSAIIQ
                     AALDEANSLNKLSFKYMDRILLNWKKNNVKTIDDSRKIREKFNKPKMTHTVKTVPKFD
                     WLNGENLDGK"
     gene            694..1353
                     /gene="nth"
                     /locus_tag="NBI57_002130"
     CDS             694..1353
                     /gene="nth"
                     /locus_tag="NBI57_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HCD1823061.1"
                     /translation="MVSKKKALEMIDVIANMFPDAECELKHDNPFELTIAVLLSAQCT
                     DVLVNRVTTELFKKYKTPEDYLAVSDEELMNDIRSIGLYRNKAKNIKKLCQSLIDQFN
                     GEIPQTHKELESLAGVGRKTANVVMSVAFDEPSLAVDTHVERVSKRLGINRWKDNVRQ
                     VEDRLCSVIPRDRWNRSHHQLIFFGRYHCLARKPKCDICPLLEDCREGQKRYKASLKE
                     A"
     gene            1358..1699
                     /locus_tag="NBI57_002131"
     CDS             1358..1699
                     /locus_tag="NBI57_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1823062.1"
                     /translation="MTNITKEVFDNLEQEIDVFAKNKALGSSEAKPYLDEYHSKIIDY
                     FKQVNDITSNIDFDNLNQYPVVPMNFKERYDYMIERKYHFMGYRQMKTFKTELIKMNA
                     SYQTRLKNKQV"
     gene            complement(2265..4448)
                     /locus_tag="NBI57_002132"
     CDS             complement(2265..4448)
                     /locus_tag="NBI57_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HCD1823063.1"
                     /translation="MTENKGSSQPKKNGNNGGKSNSKKNRNVKRTIIKIIGFMIIAFF
                     VVLLLGILLFAYYAWKAPAFTEAKLQDPIPAKIYDKNGELVKTLDNGQRHEHVNLKDV
                     PKSMKDAVLATEDNRFYEHGALDYKRLFGAIGKNLTGGFGSEGASTLTQQVVKDAFLS
                     QHKSIGRKAQEAYLSYRLEQEYSKDDIFQVYLNKIYYSDGVTGIKAAAKYYFNKDLKD
                     LNLAEEAYLAGLPQVPNNYNIYDHPKAAEDRKNTVLYLMHYHKRITDKQWEDAKKIDL
                     KANLVNRTPEERQNIDTNQDSEYNSYVNFVKSELMNNKAFKDENLGNVLQSGIKIYTN
                     MDKDVQKTLQNDVDNGSFYKNKDQQVGATILDSKTGGLVAISGGRDFKDVVNRNQATD
                     PHPTGSSLKPFLAYGPAIENMKWATNHAIQDESSYQVDGSTFRNYDTKSHGTVSIYDA
                     LRQSFNIPALKAWQSVKQNAGNDAPKKFAAKLGLNYEGDIGPSEVLGGSASEFSPTQL
                     ASAFAAIANGGTYNNAHSIQKVVTRDGETIEYDHTSHKAMSDYTAYMLAEMLKGTFKP
                     YGSAYGHGVSGVNMGAKTGTGTYGAETYSQYNLPDNAAKDVWINGFTPQYTMSVWMGF
                     SKVKQYGENSFVGHSQQEYPQFLYENVMSKISSRDGEDFKRPSSVSGSIPSINVSGSQ
                     DNNTTNRSTHGGSDTSANSSGTAQSNNNTRSQQSRNSGGLTGIFN"
     gene            complement(4445..5122)
                     /gene="recU"
                     /locus_tag="NBI57_002133"
     CDS             complement(4445..5122)
                     /gene="recU"
                     /locus_tag="NBI57_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HCD1823064.1"
                     /translation="MLCIINNVYYQTCVVKFMNYPNGKPYRKNSAIDGGKKTAAFSNI
                     EYGGRGMSLEKDIEHSNTFYLKSDIAVIHKKPTPVQIVNVNYPKRSKAVINEAYFRTP
                     STTDYNGVYQGYYIDFEAKETKNKTSFPLNNIHDHQVEHMKNAYQQKGIVFLMIRFKT
                     LDEVYLLPYSKFEVFWKRYKDNIKKSITVDEIRKNGYHIPYQYQPRLDYLKAVDKLIL
                     DESEDRV"
BASE COUNT         1736 a          828 c          883 g         1749 t
ORIGIN      
        1 taaaaggggg cacgaacatg gataaatatc aattaaaagc aagacctgta gtgatacgaa
       61 gagaattatt agaccattac agtgacttag gtttagatga gcaagattta gtcattttgc
      121 ttaaattgat ttatgcttcc gaaacatcaa ataaacagcc ttcaattgaa ttacttcaga
      181 agggctcaac tatgcaacca cgtgatatta caatggttat tcaaaatttg atacaacgtg
      241 aattgctaga attacaagtt caaaaagatg aagaaggtag atttaccgaa tatatgaatc
      301 tagatccttt tttcgaaaaa ttaagtcata tattgaagca acaaagtatg gaaacgaaag
      361 agcaaaatag taaagaaaaa tttaaacaat tatttagagt tttagaggat acatttgcaa
      421 ggccgttatc tccatatgaa atagaaacgt taaatcaatg gattgatgtt gataaacacg
      481 atagtgcaat tatacaagcg gcattagacg aggcgaatag tttgaataaa cttagcttta
      541 aatatatgga tcgtatttta cttaattgga agaaaaacaa tgtcaaaaca attgacgatt
      601 ctagaaaaat aagagaaaaa tttaataaac cgaaaatgac gcacacagtt aaaacggtac
      661 ctaaatttga ttggttaaac ggggagaacc ttgatggtaa gtaagaaaaa agcattagaa
      721 atgattgatg ttatagcaaa tatgtttccg gatgcagaat gtgaattaaa acatgataat
      781 ccgttcgaat taactattgc tgtattattg tcagcgcaat gtacagacgt tctagtaaat
      841 agagtaacaa ctgagctgtt taaaaaatac aaaacgcctg aagattattt ggcagtcagt
      901 gatgaagaac ttatgaatga tattcgttct atcggtctgt atcgtaacaa agctaagaat
      961 attaagaaac tttgtcaatc tttgattgat caatttaatg gagaaatacc acaaacacat
     1021 aaggaattag aaagtttagc aggtgtagga cgtaaaactg ctaatgtagt catgagtgta
     1081 gcatttgatg aaccttcatt agctgttgat acgcatgtag aacgtgtttc taaacgctta
     1141 ggtattaatc gttggaaaga taatgtgaga caggtagaag accgtttatg ttctgtcata
     1201 cctagagata gatggaatag gagccatcat caactcattt tctttggaag ataccactgt
     1261 ttagcccgaa aacctaaatg cgatatttgt ccactattag aagattgtag agaaggacaa
     1321 aaacgttata aagctagttt gaaagaagcg tgataacatg acaaatatta caaaagaggt
     1381 atttgataat ttagaacaag agattgatgt atttgccaaa aataaggcat taggttctag
     1441 tgaagcaaag ccctacttgg atgaatacca tagtaaaatt attgattatt ttaagcaggt
     1501 taatgacata actagcaata ttgattttga taatttaaat caatatcctg ttgtgccaat
     1561 gaattttaaa gaaagatatg attatatgat tgaacgtaaa tatcatttta tgggctatcg
     1621 acaaatgaag acctttaaaa ctgaattgat taaaatgaat gctagttatc aaacaaggtt
     1681 aaagaataag caggtataga caagattgat catttcttaa tgttgaaaat atgtaaaact
     1741 aattcatgca cacatatttg tatttatgtt aggtgtcgtg tcattacaac gtcttaatat
     1801 aaaaactgtc catctacatc ataatggata tcttgagata tgctttaaga tacctatttc
     1861 gaatcgtagg tggatttctt atatctatga ttgtgaaaat atgttgcaaa catcttgata
     1921 tttcactgat taacgtacac agggaattga taaacatttc aatttaaaac agcgtctaag
     1981 atgactatga gaaaatataa tcataatttt aaaaatggga acaaccactt attcaaattc
     2041 aataatttta catagctaaa gatgtgaata tgaaaaacta tttagatgtt tcaaaatgta
     2101 tgtgaatgac tgattttacg gtttctacta atatagcatt agcgtatgaa tacttataaa
     2161 aaaagcagga agttttacct tcccaccata aaagatgaag aacctaaaaa aatcctcagc
     2221 tacaccataa atatggtata actgaggatt ttatgttgag tggattagtt gaatatacct
     2281 gttaatccac cgctgtttct agattgttga gatctagtat tgttatttga ttgtgcagta
     2341 ccactgctgt ttgctgatgt gtcactacca ccgtgtgtac tacgatttgt agtgttgtta
     2401 tcttgactac cagaaacatt gattgatggg atactaccac ttactgagct aggacgttta
     2461 aagtcttcgc catctctaga tgaaattttt gacatcacat tttcatataa gaactgtgga
     2521 tattcttgtt ggctatgtcc cacaaatgag ttttcaccat attgtttaac tttactgaag
     2581 cccatccaca ctgacatagt gtattgaggt gtaaagccgt taatccacac gtctttcgct
     2641 gcattatcag gtaaattata ttgtgaataa gtttcagcac cgtaagtacc agtacctgtc
     2701 ttagcaccca tatttactcc agatacacca tggccatatg cagaaccata tggtttaaat
     2761 gtacctttta gcatctcagc taacatgtat gcagtgtaat cactcatcgc tttatggcta
     2821 gtatgatcgt attcgattgt ttcaccgtca cgagtaacta ctttttgaat tgaatgcgcg
     2881 ttgttataag taccaccgtt agcgattgca gcaaatgctg atgctaattg tgttggtgag
     2941 aattctgaag cagaaccacc aagtacttca gatggaccaa tatcgccttc gtagtttaag
     3001 ccaagtttgg cagcgaattt cttaggtgca tcgttaccag cattttgctt aactgattgc
     3061 catgctttta aagctgggat attgaaactt tgtcgtaaag catcataaat agatacagta
     3121 ccgtgactct tcgtatcata gtttctaaat gtagaaccgt caacttgata tgaagattca
     3181 tcttgaatcg catggtttgt tgcccatttc atattttcaa tggcaggtcc atacgctaag
     3241 aaaggtttta aagatgaacc agtagggtga ggatctgttg cttggtttct gttaacgacg
     3301 tctttgaaat cacgtccacc agatatagca actaaaccac cagttttact atcaagaatc
     3361 gttgcaccaa cttgttggtc tttattcttg tagaagctac cattatcaac atcattttgt
     3421 aatgtttttt gaacatcttt atccatgttt gtataaattt taataccact ttgtaataca
     3481 ttacctaaat tttcatcttt gaatgcttta ttattcatta attcagattt cacaaagtta
     3541 acgtatgaat tatactcaga atcttgattt gtatcaatgt tttgacgttc ttcaggagta
     3601 cgatttacta agttcgcttt taaatcgatt ttcttagcat cttcccactg tttatctgta
     3661 atgcgtttat gataatgcat taagtataaa acagtgtttt tacgatcttc agcagctttt
     3721 ggatgatcat aaatattata gttgtttgga acctgaggta aaccggctaa ataagcttct
     3781 tccgctaagt ttaaatcttt taaatcttta ttaaagtaat acttagcagc agctttaata
     3841 cctgttacgc catcagaata gtaaattttg tttagatata cttggaagat atcatcttta
     3901 ctatactctt gttctaaacg atatgataag tatgcttctt gagctttacg tccaatagat
     3961 ttatgttgtg ataaaaatgc atctttaaca acttgttgtg ttaatgttga ggcaccttca
     4021 gaaccaaatc caccagtcaa gttcttacca attgcaccga ataaacgttt ataatcaagt
     4081 gcgccatgtt cgtagaaacg attgtcttca gttgcaagta ctgcgtcttt cattgatttc
     4141 ggcacgtctt ttaaatttac atgctcatgt ctttggccat tatctaatgt tttaacaagt
     4201 tctccgttct tgtcatatat ctttgcagga atcggatctt gtaatttagc ttcggtaaaa
     4261 gcaggtgctt tccaagcata ataagcaaac aataagatac ctagtaaaag aacaacgaaa
     4321 aatgcaataa tcatgaagcc aataatctta ataatcgttc tcttcacatt tctattcttt
     4381 tttgagttgg atttcccacc attattaccg tttttcttag gctgagaaga tcctttgttt
     4441 tccgtcatac gcggtcctca ctttcatcta atatcaactt atcaactgct tttagatagt
     4501 ctaatcttgg ttgatactga taaggaatat ggtaaccatt ttttcgtatt tcatcaactg
     4561 ttatagactt tttaatatta tctttatatc tcttccaaaa tacttcgaat tttgaatagg
     4621 gtaaaagata aacttcatct agcgttttaa aacgaatcat taaaaacaca ataccttttt
     4681 gttgatatgc atttttcata tgttcgactt gatggtcatg aatattattt aaaggaaagg
     4741 acgtcttgtt tttagtttcc tttgcttcaa aatcaatata ataaccttga taaacgccgt
     4801 tgtaatcagt tgttgaaggt gtacgaaaat aagcttcgtt aatcacagct ttactccgct
     4861 taggatagtt gacattaact atttgtactg gcgtaggctt tttgtgaata actgcaatgt
     4921 cgcttttaag ataaaacgta tttgaatgtt cgatatcttt ttcaagtgac ataccacgtc
     4981 caccatactc aatattacta aaggcagcgg tctttttccc tccgtctata gcactatttt
     5041 tacgatatgg tttaccattt ggataattca taaatttcac cacacaagtt tggtagtaaa
     5101 cgttgtttat tatacacaac attatagtga aacaccaaac ttaaattagt ataaatgcga
     5161 aagacacaat acaccaaagc ttattttaac gtgatt
//
LOCUS       DAHHSC010000167         5116 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.168, whole genome shotgun sequence.
ACCESSION   DAHHSC010000167 DAHHSC010000000
VERSION     DAHHSC010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 5116)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5116)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5116
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.168"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            <1..511
                     /locus_tag="NBI57_002134"
     CDS             <1..511
                     /locus_tag="NBI57_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001558608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HCD1823065.1"
                     /translation="MFKKSVIDKNKIKFKTFSKTAEDQLFTIEFLMNSKNYSIKTDYE
                     YYIVVNDFESSNHLSVNKSTGNQYFATINEIYKAIYKSPIYKNQEKRHQLAGKYTTRL
                     LRHGQKKNFANSKMKYEDKIEWLNNFSKTINKVPRDSDKYVTQIFNLKLEAIRQNDLL
                     AVMIADKLL"
     gene            522..764
                     /locus_tag="NBI57_002135"
     CDS             522..764
                     /locus_tag="NBI57_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000427213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1823066.1"
                     /translation="MENFNYNNIKKLVLENVDIDKVKEVYKDYELLNYTIKNQTLYMN
                     DYEVAKVSEKHLNENINNLRGTVNLDEKCILSLTYL"
     gene            827..1864
                     /locus_tag="NBI57_002136"
     CDS             827..1864
                     /locus_tag="NBI57_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCD1823067.1"
                     /translation="MKTRCYDGKKWQYEFKHEGKRYRKKGFRTKREANSAGLDKLNEL
                     RSGFNIDNYITLEEYFENWIKTYKQPVVKENTYRHYRNALQHIQKHKIGKMELSKINR
                     QVYQKFINDHSKEHAKETIRKTNGAIRSALDDALYDGLIFKNPAYKVNYKAGKPTKSE
                     QEKFISVTEYEILKDHVRKKRTRSSLALFIMICTGCRVSGARNIKIEHINQVKNTIFI
                     DERKTDTSPRYISIAKSDMKHIMDVISTFAISYDGYIFKEAGSIINLQAINNALKSAC
                     RVNNIPIITSHALRHTHCSYLLAKGVSIHYISKRLGHKNIAITTSVYSHLLEEKFNEE
                     DKKTTKILESM"
     gene            1915..2745
                     /gene="sph"
                     /locus_tag="NBI57_002137"
                     /pseudo
     CDS             1915..2745
                     /gene="sph"
                     /locus_tag="NBI57_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000239616.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sphingomyelin phosphodiesterase"
     gene            complement(2983..3999)
                     /gene="lukG"
                     /locus_tag="NBI57_002138"
     CDS             complement(2983..3999)
                     /gene="lukG"
                     /locus_tag="NBI57_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bi-component leukocidin LukGH subunit G"
                     /protein_id="HCD1823068.1"
                     /translation="MIKQLYKNITICSLAISTALTVFPATSYAKINSEIKAVSEKNLD
                     GDTKMYTRTATTSDSQKNITQSLQFNFLTEPNYDKETVFIKAKGTIGSGLRILDPNGY
                     WNSTLRWPGSYSVSIQNVDDNNNTNVTDFAPKNQDESREVKYTYGYKTGGDFSINRGG
                     LTGNITKESNYSETISYQQPSYRTLLDQSTSHKGVGWKVEAHLINNMGHDHTRQLTND
                     SDNRTKSEIFSLTRNGNLWAKDNFTPKDKMPVTVSEGFNPEFLAVMSHDKKDKGKSQF
                     VVHYKRSMDEFKIDWNRHGFWGYWSGENHVDKKEEKLSALYEVDWKTHDVKFVKVLND
                     NEKK"
     gene            complement(4021..5076)
                     /gene="lukH"
                     /locus_tag="NBI57_002139"
     CDS             complement(4021..5076)
                     /gene="lukH"
                     /locus_tag="NBI57_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bi-component leukocidin LukGH subunit H"
                     /protein_id="HCD1823069.1"
                     /translation="MKNKKRVLIASSLSCAILLLSAATTQANSAHKDSQDQNKKEHVD
                     KSQQKDKRNVTNKDKNSTVPDDIGKNGKITKRTETVYDEKTNILQNLQFDFIDDPTYD
                     KNVLLVKKQGSIHSNLKFESHKEEKNSNWLKYPSEYHVDFQVKRNRKTEILDQLPKNK
                     ISTAKVDSTFSYSSGGKFDSTKGIGRTSSNSYSKTISYNQQNYDTIASGKNNNWHVHW
                     SVIANDLKYGGEVKNRNDELLFYRNTRIATVENPELSFASKYRYPALVRSGFNPEFLT
                     YLSNEKSNEKTQFEVTYTRNQDILKNRPGIHYAPPILEKNKDGQRLIVTYEVDWKNKT
                     VKVVDKYSDDNKPYKEG"
BASE COUNT         1824 a          808 c          746 g         1738 t
ORIGIN      
        1 aatgtttaaa aaaagtgtta tagataaaaa caagataaaa ttcaaaacat tctctaaaac
       61 tgctgaagac caattattta ctatagaatt tttgatgaat tcgaaaaatt actcgataaa
      121 aaccgactat gaatactata ttgtagtcaa cgatttcgag tctagcaatc atttgtcagt
      181 aaataaaagt acaggaaatc aatattttgc tactataaac gaaatttata aagctattta
      241 taaaagtcca atttataaaa accaagaaaa aagacaccaa cttgccggga agtatacaac
      301 tagactttta agacacggtc agaaaaagaa ttttgcaaat agtaaaatga aatatgaaga
      361 taaaatcgaa tggttaaaca acttttctaa aacaattaat aaagtaccta gagactcaga
      421 taaatatgtc acacaaatat tcaacttaaa attagaagca ataagacaaa acgatttatt
      481 agctgtgatg attgcagata agctattata ggaggaaaac aatggaaaac tttaattata
      541 ataacataaa aaaacttgtt cttgaaaatg ttgatataga taaagtaaaa gaagtataca
      601 aagattatga attacttaat tatacaataa aaaaccaaac actttacatg aacgattatg
      661 aagttgctaa agtttctgaa aaacatctaa acgaaaatat taataatcta agaggcacgg
      721 ttaatctaga cgaaaaatgt attttgtcac taacctatct ataatgcgaa atttcgggta
      781 gctcgcctac ccttattatt ttttgccaat tttgaggagg gaacacatga aaacacgttg
      841 ttacgatggt aaaaaatggc aatatgagtt taagcatgaa ggaaaaagat accgtaagaa
      901 aggttttaga acaaagcgtg aagctaattc tgctggacta gacaagttaa atgagttaag
      961 aagtggtttt aatatagata actatataac tcttgaagaa tacttcgaaa attggattaa
     1021 aacgtataaa caacctgttg ttaaagaaaa tacctaccgt cattatagaa atgcattaca
     1081 acatatacaa aaacataaaa taggtaaaat ggagttatca aagataaata gacaagttta
     1141 tcagaaattc ataaacgacc attcaaaaga acacgcaaaa gaaactataa gaaaaacaaa
     1201 cggtgctatt cggtcagctt tagatgacgc attatatgat ggacttattt ttaaaaaccc
     1261 cgcttataaa gttaattata aagccggaaa acctacgaag tcagaacaag aaaaattcat
     1321 ctcggtaact gaatatgaaa tactaaaaga tcacgtcaga aagaagagaa ctcgttcatc
     1381 attagcgcta ttcataatga tttgtacggg ttgtcgtgtc agtggtgcaa gaaatataaa
     1441 gattgagcat atcaaccaag tgaaaaacac tatatttatt gacgagcgaa aaaccgatac
     1501 ttcccctaga tatatcagta tcgctaaatc tgatatgaaa cacattatgg acgtcataag
     1561 tacatttgca attagctatg atggttacat tttcaaagaa gccggatcta taattaacct
     1621 tcaggctatc aataatgctt tgaaatcagc ctgtagagtc aataatatac caattattac
     1681 atcgcacgca ttaagacaca ctcattgttc ttatttacta gcaaaaggtg tatctataca
     1741 ttacatttct aaaagattag gtcataaaaa tatagcaata actacatccg tgtattctca
     1801 tttgttagaa gaaaaattta atgaagagga caaaaaaaca actaaaattt tagaaagtat
     1861 gtaatttagg gacccattag ggactccaaa cccaataaat actgttgtta caaggtttct
     1921 atgtatccaa actggggaca atataaacgc gctgatttaa tcggacaatc ttcttatatt
     1981 aaaaataatg atgtcgtaat attcaatgaa gcatttgata atggtgcatc agacaaatta
     2041 ttaagtaatg tgaaaaaaga atatccttac caaacacctg tactcggtcg ttctcaatca
     2101 ggttgggaca aaactgaagg tagctactca tcaactgttg ctgaagatgg tggcgtagcg
     2161 attgtaagta aatatcctat taaagaaaaa atccagcatg ttttcaaaag cggttgtgga
     2221 ttcgataatg atagcaacaa aggctttgtt tatacaaaaa tagagaaaaa tggtaagaac
     2281 gttcacgtta tcggtacaca tacacaatct gaagattcac gttgtggtgc tggacatgat
     2341 cgaaaaatta gagctgaaca aatgaaagaa atcagtgact ttgttaaaaa gaaaaatatc
     2401 cctaaagatg aaacggtata tataggtggc gaccttaatg ttaataaagg cactccagag
     2461 ttcaaagata tgcttaaaaa cttgaatgta aatgatgttc tatatgcagg tcataatagc
     2521 acatgggacc ctcaatcaaa ttcaattgcg aaatataatt accctaatgg taaaccagaa
     2581 catttagact atatatttac agataaagat cataaacaac caaaacaatt agtcaatgaa
     2641 gttgtgactg aaaaacctaa gccatgggat gtatatgcgt tcccatatta ctacgtttac
     2701 aatgattttt cagatcatta cccaatcaaa gcctatagta aatagtgctc aactaactaa
     2761 taactcgctt cgttctaaaa ggacgaagcg agttatattg ttaaaatttg aattgactta
     2821 cattttaata aaatcatctt aacaacttta atttttcatt aatacaagtc tttactctac
     2881 actcaaacaa gattcataca ctgcacatca taataaatct atctactcaa atataaataa
     2941 aagttaccta ctacattcta tgtagcaggc aacttttatt acttatttct tttcattatc
     3001 attaagtact tttacaaact tcacatcatg tgtcttccaa tcaacttcat ataatgctga
     3061 taatttttct tcttttttat ctacatggtt ttcaccagac caatagcccc agaaaccatg
     3121 gcgattccaa tctattttaa actcatccat tgatctttta taatgaacaa caaattgtga
     3181 tttacctttg tcttttttat catgtgacat aacagctaaa aattctggat taaacccttc
     3241 agacacagtt acaggcattt tgtctttagg tgtgaaatta tctttcgccc ataaatttcc
     3301 atttcgtgtt aaagaaaaga tttcactttt agttctatta tcactatcat tagttaattg
     3361 tctcgtatgg tcatgtccca tattatttat caaatgtgct tctactttcc aacctacacc
     3421 tttatgtgac gtagattgat caagtaatgt acgatatgat ggttgttgat aactaatcgt
     3481 ctctgaataa ttactctctt ttgtaatatt tccagttaag cctccacgat taatcgaaaa
     3541 atctccacct gttttataac catacgtata tttaacttct cttgattcat cctgattttt
     3601 tggtgcaaag tcagtcacat ttgtattgtt gttgtcatca acattttgaa ttgaaactga
     3661 ataagatcca ggccatctta atgtactatt ccaataacca tttgggtcta aaattctcaa
     3721 accactacca attgtacctt ttgctttaat aaatactgtt tctttatcat aattaggttc
     3781 agttaagaaa ttaaattgta agctttgagt aatatttttt tgactatcac ttgttgtagc
     3841 tgtacgtgta tacattttag tatcaccatc aagattcttc tcagaaacag ctttaatttc
     3901 agaattaatt tttgcataag aagttgccgg aaatacagtt aatgcagtag atattgctaa
     3961 actacaaatt gtgatgtttt tgtataattg tttaatcatt agtaatccgc cctttcaata
     4021 ttatccttct ttataaggtt tattgtcatc agaatattta tcaacgactt taactgtttt
     4081 atttttccaa tcaacttcat aagtgacaat taacctttga ccatctttat ttttttctaa
     4141 aattggaggt gcataatgta ttccaggtct gtttttcaaa atatcttgat ttcgtgtgta
     4201 tgttacttca aattgcgttt tctcatttga cttttcatta gataaataag ttaaaaattc
     4261 tggattaaag ccacttctta ctaatgctgg gtatctatat tttgaagcaa agcttagttc
     4321 agggttttct acagtagcaa ttctcgtatt tctatagaat aataattcat catttctatt
     4381 tttcacttct ccaccatact tcaagtcatt cgcaataact gaccagtgta catgccagtt
     4441 attattttta ccgctggcaa ttgtgtcata attttgctga ttataactaa tcgttttgga
     4501 gtagctattt gatgaagttc gtccaatacc tttcgttgaa tcgaatttac cacctgagct
     4561 atatgaaaat gtactgtcta ctttcgcagt tgaaatttta tttttcggca attggtctaa
     4621 tatttcagtt ttacgatttc tttttacttg aaaatctaca tggtactcac ttggatactt
     4681 taaccaattt gaattttttt cttctttatg agattcaaac tttaaatttg aatgaattga
     4741 gccttgtttt ttaacaagta atacattctt gtcataagtt ggatcatcga taaagtcgaa
     4801 ttgtaaattt tggagtatat ttgttttctc atcatatact gtttcagttc gttttgtgat
     4861 tttaccgttt ttcccaatat catcaggtac tgttgaattt ttatctttat tagtaacatt
     4921 acgtttgtct ttttgttgag acttatcaac atgttctttc ttattttgat cttgcgagtc
     4981 tttatgagct gaatttgctt gagtcgttgc tgctgataac aataaaattg cacatgataa
     5041 tgatgacgct attaaaacac gttttttatt tttcattgtt ataaccttct ttcgtatgat
     5101 tgatatttgt tgatat
//
LOCUS       DAHHSC010000168         5090 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.169, whole genome shotgun sequence.
ACCESSION   DAHHSC010000168 DAHHSC010000000
VERSION     DAHHSC010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 5090)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5090)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5090
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.169"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            complement(203..760)
                     /locus_tag="NBI57_002140"
     CDS             complement(203..760)
                     /locus_tag="NBI57_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000606891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HCD1823070.1"
                     /translation="MINFNKTALVLIDLQEGILKMDYAPHTAENVVQNANKLIDVFRK
                     NNGFIAFVRVNFYDGKDVLQPNAMISLPPKEGDDYSRFHHLLDKRDDDFVIDKRHFSA
                     FVGTDLDLQLRRRGIDTIVLGGVATHIGVDTTARDAYQLNYNQFFVTDMMSAQNETLH
                     QFPIDNVFPLMGQTITTNDFLNILN"
     gene            complement(827..2467)
                     /locus_tag="NBI57_002141"
     CDS             complement(827..2467)
                     /locus_tag="NBI57_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-keto acid decarboxylase family protein"
                     /protein_id="HCD1823071.1"
                     /translation="MKQRIGAYLIDAFHRAGVDKIFGVPGDFNLAFLDDIISNPNVDW
                     VGNTNELNASYAADGYARLNGLAALVTTFGVGELSAVNGIAGSYAERIPVIAITGAPT
                     RAVEQAGKYVHHSLGEGTFDDYRKMFAHITVAQGYITPENATTEIPRLINTAIAERRP
                     VHLHLPIDVAISEIEIPTPFEVTATKDTDASTYIELLATKLHQAKQPIIITGHEINSF
                     HLHQELEDFVNQTQIPVAQLSLGKGAFNEENPYYMGIYDGKIAEDKIRDYVDNSDLIL
                     NIGAKLTDSATAGFSYQFNIDDVVMLNHHNIKIDDVTNDEISLPSLLKQLSNISYTNS
                     ATFPAYHRPTSPDYTVGTEPLTQQTYFKMMQNFLKPNDVIIADQGTSFFGAYDLALYK
                     NNTFIGQPLWGSIGYTLPATLGSQLADKDRRNLLLIGDGSLQLTVQAISTMIRQHIKP
                     VLFVINNDGYTVERLIHGMYEPYNEIHMWDYKALPAVFGGKNVEIHDVESSKDLQDTF
                     NAINGHPDVMHFVEVKMSVEDAPKKLIDIAKAFSQQNK"
     gene            2660..2761
                     /locus_tag="NBI57_002142"
     CDS             2660..2761
                     /locus_tag="NBI57_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1823072.1"
                     /translation="MMPQNTIAKKKQARHLMKVYAPVYSKHYLASNN"
     gene            complement(2740..4785)
                     /locus_tag="NBI57_002143"
     CDS             complement(2740..4785)
                     /locus_tag="NBI57_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIABC"
                     /protein_id="HCD1823073.1"
                     /translation="MRKKLFGQLQRIGKALMLPVAILPAAGLLLAIGTAIQGEALQHY
                     LPFIQNGGVQNVAKLMTAAGSIIFENLPMIFALGVAIGLAGGDGVAAIAAFVGYIIMN
                     KTMGDFLQVTPKNVTDPASGYASILGIPTLQTGVFGGIIIGALAAWCYNKFYNINLPS
                     YLGFFAGKRFVPIMMATTSFILAFPMALIWPTIQSGLNAFSTGLLDSNTGVAVFLFGF
                     IKRLLIPFGLHHIFHAPFWFEFGSWKNAAGEIIHGDQRIFIEQIREGAHLTAGKFMQG
                     EFPVMMFGLPAAALAIYHTAKPENKKVVAGLMGSAALTSFLTGITEPLEFSFLFVAPL
                     LFFIHAVLDGLSFLTLYLLDVHLGYTFSGGFIDYVLLGVLPNKTQWWLVIPVGLVYAV
                     IYYFVFRFLIVKLKYKTPGREDKQSQAVTASATELPYAVLEAMGGKANIKHLDACITR
                     LRVEVNDKSKVDVPGLKDLGASGVLEVGNNMQAIFGPKSDQIKHEMQQIMNGQVVENP
                     TTMEDDKDETVVVAEDKSATSELSHIVHAPLTGEVTPLSEVPDQVFSEKMMGDGIAIK
                     PSQGEVRAPFNGKIQMIFPTKHAIGLVSDSDLELLIHIGLDTVKLNGEGFTLHVEEGQ
                     EVKQGDLLINFDLDYIRNHAKSDITPIIVTQGNITNLDFKQGEHGNISFGDQLFEAK"
BASE COUNT         1602 a          917 c          916 g         1655 t
ORIGIN      
        1 gatacataat tttgtaataa atactaaatt ttttgaaaat tccggaataa atactgtttt
       61 ttatgtagta ggggcgtaag ttaagaacga tttaatagat ataggctaag tttatagata
      121 ggaggttaga caacccgacg tattaatact ttgtaaaata aagatacccc cttcaacatg
      181 atcgaagggg gtaagtgtat gtttagttca atatatttag aaagtcgttt gtagttattg
      241 tttgtcccat taatgggaat acattatcta ttggaaattg atgtagcgtt tcgttttgtg
      301 cactcatcat atctgtaaca aaaaactgat tgtagtttaa ttgataggca tctcgcgctg
      361 tcgtatctac gccaatatgc gttgcgacac caccaagaac aatcgtatca attcctcgac
      421 gtcgcaattg taagtccaaa tctgttccta caaatgcact aaaatgtcgt ttgtctatga
      481 caaaatcgtc atctctcttg tctaataaat gatggaaacg actgtagtcg tcgccttctt
      541 ttggtggtaa tgagatcatt gcatttggtt gcaatacatc tttaccatca tagaaattca
      601 cgcgaacaaa agcgataaag ccattgtttt ttctaaaaac atctattaat ttattagcgt
      661 tttgaacgac attttcagct gtatgtggcg cataatccat cttaagtata ccttcttgca
      721 ggtcgattaa cactaaagcg gttttattaa aattaatcat cttatatcag agtccttttc
      781 gtttaataaa gtattcgctt aaacttatac cctgtatacg atgaaattat ttattttgtt
      841 gtgaaaaagc tttagcgata tcgatcagtt tcttcggtgc gtcttcgaca gacattttga
      901 cttcgacaaa atgcatcaca tcgggatgac cattaattgc attaaacgtg tcttgtaaat
      961 cttttgatga ttcaacgtca tgaatttcaa catttttacc accaaataca gctggtaaag
     1021 ctttataatc ccacatgtga atttcattat aaggttcata catgccgtga ataagtcgtt
     1081 ctaccgtata gccgtcatta ttaatcacaa ataataccgg tttaatatgc tgtctaatca
     1141 tagttgaaat agcttgaaca gttagttgca atgagccatc accaattaat aataagttac
     1201 gacgatcttt gtctgctaat tgtgaaccta atgttgcagg taatgtatag ccgatagaac
     1261 cccataacgg ttgccctata aaagtattgt ttttgtataa tgctaaatca taagcaccaa
     1321 agaatgatgt accttgatca gcaataatga catcatttgg ttttaagaaa ttttgcatca
     1381 ttttaaaata agtttgttgt gttaatggtt ctgtgccaac agtataatcg ggtgatgttg
     1441 gacgatgata cgcagggaac gttgcactgt tcgtatatga aatattggat aactgtttta
     1501 acaacgatgg tagagatatt tcatcatttg taacatcatc aattttgata ttgtgatgat
     1561 ttaacataac gacatcatcg atattgaatt ggtatgaaaa tcctgctgtt gctgaatctg
     1621 ttaatttggc tccaatattt aaaattaaat cgctgttgtc cacataatct cgtattttat
     1681 cttcggcaat tttcccgtcg taaataccca tataatatgg attttcctca ttaaaagcac
     1741 cttttcctaa tgaaagttgt gctactggta tctgtgtttg atttacaaaa tcttctaatt
     1801 cttgatggag gtgaaaactg ttaatttcat gtccagtaat gatgataggc tgcttcgctt
     1861 gatgcagttt agttgctaat aactctatat atgttgatgc atccgtatct ttagttgccg
     1921 tcacttcaaa tggtgtcggt atctcaattt cagagattgc gacatcgatt ggtaaatgta
     1981 aatgaactgg gcgtctttcg gcgattgctg tattaattaa acgtggtatt tcggttgttg
     2041 cattttcagg tgtgatataa ccttgtgcaa cggttatatg tgcaaacatt tttcggtagt
     2101 cgtcaaatgt accttcacca agtgagtgat gtacatattt accggcttgt tcaacagcac
     2161 gtgtcggcgc acctgtaatc gcaatgacag gtatgcgttc agcatatgaa cctgcgatac
     2221 cgttgacggc acttaattcg ccaacaccaa atgtagtaac taatgcagcg agtccattaa
     2281 gacgggcata accgtccgct gcgtaacttg cgtttaattc atttgtattt cctacccaat
     2341 ctacattggg attgctgata atatcgtcta gaaaagcgag attaaaatca ccaggaacac
     2401 caaaaatttt atcgacgcct gctcgatgaa aagcgtcaat taagtaagct ccaatgcgtt
     2461 gtttcataaa aaaaccacct tttctctata tttacactta aaatgatagc ctcgaataca
     2521 gtgtaaggtc aaggaagttg actttgttgt gaaaacgcta acaatagtga acaattaata
     2581 ctttttagga aatgagtatg aaggggggct taaattgatg atcttaaaat tcaaaattta
     2641 aggggagagc gaactatcaa tgatgccgca aaatacaata gcaaagaaaa aacaggcgcg
     2701 tcaccttatg aaggtatacg cacctgttta tagtaagcat tatttagctt caaataattg
     2761 atcgccaaat gaaatgttgc catgttcacc ttgtttaaaa tcaaggtttg taatgtttcc
     2821 ttgtgtcacg ataataggcg taatatcact ctttgcatga ttgcggatgt agtctaaatc
     2881 aaagttgatt aataaatcac cttgtttaac ttcttgacct tcctcaacat gtaaagtaaa
     2941 gccttctccg tttaatttaa cagtgtctaa accgatgtgg attaatagtt ctaaatcact
     3001 atctgataca agaccaattg catgttttgt tgggaaaatc atttgtattt taccgttgaa
     3061 tggtgcacga acttcacctt gtgaaggttt aatagcgata ccgtcaccca tcattttttc
     3121 gctgaacact tgatcaggca cttctgataa aggtgttact tcaccagtta atggtgcatg
     3181 aacgatatgg ctcaattcgc ttgttgcaga tttatcttct gcaacaacaa cagtttcgtc
     3241 tttatcgtct tccatagtag tagggttttc tactacttga ccattcataa tctgttgcat
     3301 ttcatgtttg atttggtcag atttaggacc aaaaattgct tgcatattat tgccgacttc
     3361 taatacacca gatgcgccta aatctttcaa accaggaaca tcaactttag atttgtcgtt
     3421 aacttcaaca cgtagacgtg tgatacaagc gtctaaatgt ttaatgtttg ctttgccacc
     3481 catagcttct aatactgcat atggtaattc agttgctgaa gcagtaacag cttgtgattg
     3541 cttatcttca cgacctggtg ttttgtattt taattttaca attaagaatc ggaatacgaa
     3601 gtagtaaata actgcgtata caagacctac aggaatgact aaccaccatt gtgtcttatt
     3661 aggtagtacg ccaagtaaaa cgtagtcgat gaaaccacct gagaaagtat aacctaagtg
     3721 aacatccaat aagtacaatg ttaagaatga taaaccatca agtactgcgt gaataaagaa
     3781 taataatggt gctacgaata agaatgagaa ttctaaaggt tctgtaatac cagttaagaa
     3841 tgatgttaaa gcagcagaac ccattaaacc tgctactact ttcttatttt caggtttagc
     3901 tgtgtgataa attgctaaag ctgctgcagg taaaccgaac atcataacag ggaattcacc
     3961 ttgcatgaat ttaccagctg tcaaatgtgc gccttcacga atttgttcga taaagatacg
     4021 ttggtcaccg tgaataattt caccagctgc atttttccat gaaccaaact cgaaccagaa
     4081 cggtgcgtgg aaaatgtgat gtagaccgaa tggaattaat aaacgcttga tgaaaccaaa
     4141 taagaatacg gcaacaccag tatttgaatc taataatcct gtactgaatg catttaatcc
     4201 tgattgaatc gttggccaaa ttaatgccat tggaaatgct aaaataaatg atgttgtagc
     4261 catcataata ggtacgaaac gcttaccagc gaagaaacct aaataagatg gtaagttaat
     4321 gttatagaac ttgttataac accaagctgc cagggccccg ataataatac cgccgaacac
     4381 acctgtttgt aatgttggaa tacctaaaat gctagcgtaa ccactcgctg gatcagtaac
     4441 atttttaggt gtaacttgta aaaagtcgcc cattgttttg ttcatgatta tgtaaccgac
     4501 gaatgctgcg atagctgcta cgccatcacc gccagctaat ccgattgcga cacctaatgc
     4561 gaaaatcata ggtaagtttt caaaaataat actaccggca gctgtcatta atttagcgac
     4621 attttgaacg ccaccatttt gtataaacgg caagtagtgt tgtaatgctt cgccttgtat
     4681 agctgtaccg atagctaata acagacccgc tgctggtaaa attgcaacag gtaacattag
     4741 cgctttacca atacgttgca attgaccgaa aagtttcttc ctcacttgtc caacctccaa
     4801 agttgtatgt aatatttaag attcaataaa aaagacacga gcaaatagac tgtgttggta
     4861 accatcacag cttaatttaa ctcatgccta atcttactta gtaacacgtt ggtgtatgta
     4921 attaatgtaa aagaagcaat tgactaagta gtatataaaa ctgatacgtt agtttatcta
     4981 gcttaccatc acatcttatt gaatcttatt tgtttgcgac tcctatttta gcatggtgaa
     5041 gtatgcgttt tcaatacaat tattaaattc aatttcaaat ctcttaaatg
//
LOCUS       DAHHSC010000169         5051 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.170, whole genome shotgun sequence.
ACCESSION   DAHHSC010000169 DAHHSC010000000
VERSION     DAHHSC010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 5051)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5051)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5051
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.170"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            complement(161..1867)
                     /gene="acsA"
                     /locus_tag="NBI57_002144"
     CDS             complement(161..1867)
                     /gene="acsA"
                     /locus_tag="NBI57_002144"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HCD1823074.1"
                     /translation="MKVEVYKGAQGKHNLKDYEETYNTFDWKDVEQAFSWSETGKMNM
                     AYECIDRHVDQGLGDKIALNYKDEYRKESYTYKDMQRLSNKAANVLSEHAEVDKGDRV
                     FIFMSRTPELYFALLGVLKIGAIVGPLFEAFMEKAVADRLENSEAKVLITNKALLPRV
                     PVDKLPNLKKIVVVDEDVEDNYIDFISLMETASDEFDIEWLKSDDGLILHYTSGSTGQ
                     PKGVLHVQQAMLVHYISGKYVLDLQEDDVYWCTADPGWVTGTSYGIFAPWLNGATNCI
                     AGGRFSPEQWYSMIEDFKVTIWYTAPTALRMLMSAGDDIVEKYDLSSLRSILSVGEPL
                     NPEVIKWAKKVYGLTVLDTWWMTETGGHMIVNYPTMDVKLGSMGKPLPGIQAAIIDDA
                     GNELPPNRMGNLAIKKGWPSMMYRIWKNPEKYKSYFIGDWYVSGDSAYKDEDGYFWFQ
                     GRVDDVIMTAGERVGPFEVESKLVEHEAVAEAGIIGKPDPVRGEIIKAFVALRKGYEP
                     TDELKEEIRLFVKEGLSAHAAPREIEFKDKLPKTRSGKIMRRVLKAWELNLDAGDLST
                     ME"
     gene            2036..2668
                     /locus_tag="NBI57_002145"
     CDS             2036..2668
                     /locus_tag="NBI57_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCD1823075.1"
                     /translation="MNHLKTYQSEDYYIHDKQFVIEGPLTYEDLKALTFDAHLTAFRD
                     AEDQYEALLEITTLPEGRIYVARQDELIVGYVTFHYPDEIERWSTGNLPYLIELGAIE
                     VSINFRQLHLAEKLIQLSLSTPEFEDYIVITTEYYWHWDLKNSKLDVFDYKKLMQRLM
                     ATGGLEIFATDDPEITSHPANCLMARIGKNITLEQQQAFDDIRYMNRFFF"
     gene            2693..3862
                     /locus_tag="NBI57_002146"
     CDS             2693..3862
                     /locus_tag="NBI57_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetoin utilization protein AcuC"
                     /protein_id="HCD1823076.1"
                     /translation="MQQHSSKTAYVYSDKLLQYRFHDQHPFNQMRLKLTTELLLNANL
                     LSPEQIVQPRIATGDELMLIHKYDYVEAIKHASHGIISEDEAKKYGLNDEENGQFKHM
                     HRHSATIVGGALTLADLIMSGKVLNGCHLGGGLHHAQPGRASGFCIYNDIAITAQYLA
                     KEYNQRVLIIDTDAHHGDGTQWSFYADNHVTTYSIHETGKFLFPGSGHYTERGEDIGY
                     GHTVNVPLEPYTEDASFLECFKLTVEPVVKSFKPDIILSVNGVDIHYRDPLTHLNCTL
                     HSLYEIPYFVKYLADSYTNGKVIMFGGGGYNIWRVVPRAWSHVFLSLIDQPIQSGYLP
                     LEWINKWKHYSSELLPKRWEDRLNDYTYVPRTKEISEKNKKLALHIASWYESTRQ"
     gene            complement(3960..4949)
                     /gene="ccpA"
                     /locus_tag="NBI57_002147"
     CDS             complement(3960..4949)
                     /gene="ccpA"
                     /locus_tag="NBI57_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HCD1823077.1"
                     /translation="MTVTIYDVAREARVSMATVSRVVNGNQNVKAETKNKVNEVIKRL
                     NYRPNAVARGLASKKTTTVGVIIPDISNIYYSQLARGLEDIATMYKYHSIISNSDNDP
                     EKEKEIFNNLLSKQVDGIIFLGGTITEEMKELINQSSVPVVVSGTNGKDAHIASVNID
                     FTEAAKEITGELIEKGAKSFALVGGEHSKKAQEDVLEGLTEVLNKNGLQLGDTLNCSG
                     AESYKEGVKAFAKMKGNLPDAILCISDEEAIGIMHSAMDAGIKVPEELQIISFNNTRL
                     VEMVRPQLSSVIQPLYDIGAVGMRLLTKYMNDEKIEEPNVVLPHRIEYRGTTK"
BASE COUNT         1590 a          975 c          743 g         1743 t
ORIGIN      
        1 gttatgtcat cattttcaaa tgctttctac gaaatgatat gacacaagtt aacagctctt
       61 tatattgctt atcgtaaagt aaacgattat ttaaacattg acatgaaata tgttgtcacc
      121 ctttattctt cgaaaaaaat cttcaataac attcatgtca ttattccatt gtacttaaat
      181 ccccagcatc taaatttaat tcccaagctt ttaatacacg tctcataatt ttacctgacc
      241 gtgttttagg taatttatct ttaaattcga tttcacgtgg tgctgcatgt gccgacaaac
      301 cttctttaac aaataaacga atttcttctt ttaattcgtc tgttggttca tatccttttc
      361 tcagtgcaac aaacgcctta attatttcac cgcgaaccgg atcaggttta ccaataattc
      421 ctgcttcggc aactgcttcg tgttcaacca atttagactc aacctcaaat ggtccaactc
      481 gttcaccagc tgtcataatt acatcatcaa cacgtccttg gaaccagaag taaccatctt
      541 catctttata tgccgaatca ccagatacat accagtctcc aataaaatat gatttatatt
      601 tttctggatt cttccagata cgatacatca ttgatggcca gccttttttt atagcaaggt
      661 tgcccattcg atttggtggt aattcattac ctgcatcatc gataattgca gcttgaatac
      721 caggtaatgg tttgcccatt gagccaagct tgacgtccat cgttggatag ttaacaatca
      781 tatgtccacc tgtttctgtc atccaccaag tatctaacac cgttaaaccg tatacttttt
      841 tcgcccattt tataacttca ggatttaaag gctcacctac tgatagaatc gaacgtaacg
      901 atgacaagtc atatttctca acaatatcgt caccagcact cattaacatt cttaaagctg
      961 ttggtgccgt ataccaaatc gtcactttaa aatcttcaat catactatac cactgttctg
     1021 gtgaaaagcg accaccagct atacaatttg tagcgccatt taaccatggt gcaaaaatac
     1081 cataagatgt tcctgtaacc caacctggat ctgctgtaca ccaataaaca tcatcttctt
     1141 gtaaatctaa tacatatttt ccagaaatat agtgcactaa cattgcttgt tgaacatgca
     1201 atacaccttt aggttgccca gtagaacctg atgtataatg taaaatcaaa ccatcatccg
     1261 actttaacca ttcaatgtca aattcatcgc tagcagtttc catcaaacta atgaagtcta
     1321 tgtaattgtc ttctacatcc tcatctacga caacaatttt tttcaagttc ggtaatttat
     1381 ctacaggtac tcgaggtaac aatgccttat tagtaattaa cactttagct tcactgttct
     1441 ctaatctatc cgcaactgcc ttttccataa atgcttcaaa taacggccca acaattgcac
     1501 caatttttaa aacacctaac aacgcaaaat atagttcagg tgtacgcgac ataaatataa
     1561 atactctgtc acctttgtca acttctgcat gttcagacaa aacattcgct gctttattag
     1621 ataaccgttg catatcttta taagtatacg attcttttct gtactcatct ttgtaattta
     1681 acgctatttt atcccctaat ccttgatcta catggcgatc tatacattca tatgccatgt
     1741 tcatttttcc agtttcactc caagaaaatg cttgttctac gtctttccaa tcaaaagtat
     1801 tatatgtttc ttcataatct ttaaggttat gtttaccttg cgctccttta taaacttcga
     1861 ctttcatttg aaactccccc tttgttatgt gaaaacgctt tctttctcaa tattatacat
     1921 aacatttcta attattcaaa atttttactt ttatcctttt tctagtatta attattgtaa
     1981 attttctata taatgaattt aatacaatca cgacgtcgat tgaggagttg aaaatatgaa
     2041 tcatttaaag acgtatcaat ccgaagatta ttacattcat gacaagcaat ttgttattga
     2101 aggtccttta acatacgaag atttgaaagc gcttactttc gatgcgcatt taaccgcatt
     2161 tagagatgct gaagatcagt atgaagcttt gttagaaatt acaacattac cagaaggtag
     2221 aatttatgtt gctcgccaag atgaactcat tgtgggttat gtcactttcc actatcctga
     2281 tgaaattgag cgctggtcta caggtaacct tccatattta atcgaattgg gggcaattga
     2341 agtcagcatc aattttaggc aattacatct tgcagaaaag ctaatacaac ttagcctttc
     2401 tacaccagaa ttcgaggatt atatcgttat aactactgaa tattactggc attgggattt
     2461 aaaaaattca aagttagatg tatttgacta taaaaaatta atgcagcggt taatggcaac
     2521 tggtggactt gaaatattcg ctacagatga tccagaaata acaagtcatc cagctaattg
     2581 tttaatggca agaattggca aaaatattac attagaacag caacaagcgt ttgatgatat
     2641 tcgttatatg aatcggtttt tcttttaatt tttggaatat cggagggatt atatgcaaca
     2701 acattcatca aaaactgcat atgtttattc agataagtta ttacaatatc gatttcatga
     2761 ccaacatccc ttcaatcaaa tgcgtttaaa attaacaaca gagctacttt tgaatgcaaa
     2821 tttattgtct ccagaacaaa tagtacaacc tagaattgca acaggtgacg aattaatgtt
     2881 aattcataaa tatgattacg tcgaagctat taagcatgct tcacatggca ttatcagtga
     2941 agatgaggct aagaaatatg gattaaatga tgaagagaat ggtcaattta agcatatgca
     3001 ccgccatagt gccacaattg ttggaggcgc tttaacttta gcagatctta ttatgtcagg
     3061 caaagtatta aatggttgtc acttaggtgg tggtttgcat cacgctcaac ctggtcgagc
     3121 aagtggtttt tgtatataca atgatattgc aattaccgca caatacttag ctaaagaata
     3181 caatcaacgc gttttaatca tagataccga tgcacatcat ggagatggta cacaatggag
     3241 tttctatgcc gataaccatg ttactactta ttctatccat gaaaccggaa agtttctttt
     3301 cccaggctct ggtcactata ctgaacgcgg tgaagatatc ggctatggac acactgtaaa
     3361 tgtcccactt gaaccgtata cagaagatgc atcatttttg gagtgtttta aattaacagt
     3421 tgagcctgtc gtaaagagtt ttaaacctga tattattcta agcgtaaatg gtgtcgatat
     3481 acattatcgt gatccactaa ctcatctaaa ttgtacgtta cattcattat atgaaattcc
     3541 atattttgta aaatatttag ctgattctta tacgaatgga aaggtaatta tgtttggtgg
     3601 cggaggctac aatatttgga gagtcgtacc acgtgcatgg agtcatgtat tcttaagttt
     3661 aattgatcaa ccaattcaaa gtggttattt accgttagaa tggattaata aatggaaaca
     3721 ttattcatct gaattattac ctaaaagatg ggaagatcgt ttaaatgatt atacctatgt
     3781 cccccgcaca aaagaaatta gtgaaaaaaa taaaaaatta gctttacata tagcgagttg
     3841 gtacgaatct actcgtcaat aagtaaccta atctgcaacg attttaaaag ataaataaaa
     3901 ggcattcttc caacacccat ataatttggg tcgttagatg aatgcctaat tttgtgaatt
     3961 tattttgtag ttcctcggta ttcaattctg tgaggtaaaa ctacatttgg ttcttctatc
     4021 ttttcatcgt tcatatattt tgttaataag cgcatcccta ctgcaccgat atcatataat
     4081 ggttgaataa cactagaaag ttgtggtcta accatctcaa ctaatcgtgt attattgaaa
     4141 ctaataattt gtaattcctc tggaacttta ataccagcat ccattgcact atgcataata
     4201 ccaattgctt cttcgtcgct gatacataaa atggcatctg gcaaattgcc tttcatttta
     4261 gcaaaagctt ttacgccttc tttataactt tcagcaccag aacaattcaa tgtatcacct
     4321 aattgaaggc catttttatt taacacttca gttaaacctt ctaaaacatc ttcttgagct
     4381 tttttagaat gttctccacc tactaaagca aatgatttag cgcctttttc aattaattct
     4441 cccgtaattt ctttcgcagc ttcagtaaaa tcaatattaa ctgatgctat atgtgcatcc
     4501 ttaccatttg ttcctgatac tactacaggt acagatgatt gatttatcaa ttctttcatt
     4561 tcttcagtaa ttgtaccacc aaggaaaata ataccatcaa cctgtttact taataagtta
     4621 ttaaaaattt ctttttcctt ttcaggatcg ttatctgaat ttgaaataat tgagtgatat
     4681 ttatacattg ttgcaatatc ttcaagtcca cgagcaagtt gtgaataata gatattagat
     4741 atatctggaa tgatcacacc tactgttgtt gtctttttac tagctaaacc tctagcaaca
     4801 gcatttggac gataattcaa acgcttaatg acttcgttaa ctttattttt agtttctgct
     4861 ttaacatttt ggttcccatt aacaacacgc gacactgtgg ccatagagac acgcgcttct
     4921 cttgctacat catatatagt aactgtcata atttcctcct tgtaaacgtt ttattcatta
     4981 taataacatg ttttcatttc attttcatag tttaccatat tttaatttac agtaacatat
     5041 ttttgtttgg a
//
LOCUS       DAHHSC010000170         5029 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.171, whole genome shotgun sequence.
ACCESSION   DAHHSC010000170 DAHHSC010000000
VERSION     DAHHSC010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 5029)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5029)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5029
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.171"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            <1..273
                     /locus_tag="NBI57_002148"
     CDS             <1..273
                     /locus_tag="NBI57_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1823078.1"
                     /translation="LQLNLSKGLIDDVNAQTFIKSNQSGIVISVICCLLITGILYYTL
                     DGFYASLTGVLGTLVALNPYTTIVSAVLYILTAIATVEATKTKYRN"
     gene            469..1854
                     /gene="fumC"
                     /locus_tag="NBI57_002149"
     CDS             469..1854
                     /gene="fumC"
                     /locus_tag="NBI57_002149"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000116229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HCD1823079.1"
                     /translation="MSVRIEHDTFGEIEVPADKYWGAQTERSKRNFPVGKERMPIEVV
                     YGFAQLKRAAALANFDLGKLSEAKKDAIVYACDQILSGELDEHFPLVVWQTGSGTQSN
                     MNVNEVVSYVANMYLKDHQIDESIHPNDDVNESQSSNDTFPTAMHVALYQEVETKLEP
                     ALKLLRNTLKEKEDKFESIIKIGRTHLQDATPIKLGQEISGWRYMLDRCEIMLSESKK
                     HILNLAIGGTAVGTGINAHPEFGDKVAHYISENTGYPFVSSENKFHALTAHDEVVQLH
                     GTLKALAGDLMKIANDVRWLASGPRAGLAEISIPENEPGSSIMPGKVNPTQCEMLTMV
                     AVQVMGNDTVVGFASSQGNFELNVYKPVIMHNTLQSIYLLADGMETFNNNCAVGIEPI
                     EENIDNYLNQSLMLVTALNPHIGYEKAAQIAKKAHKEGLTLKESAIQTGYVTEEQFEA
                     WIKPEDMVDPH"
     gene            complement(2307..3128)
                     /locus_tag="NBI57_002150"
     CDS             complement(2307..3128)
                     /locus_tag="NBI57_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HCD1823080.1"
                     /translation="MKFKIPENFNDLSLRDIFQQLKVPKKDLHHLNMSKDITINDKPA
                     RLMDKVHTGDDVFVPTIDEKSNYVPSYRYAQIKYEDDDMAIVMKPKGVKTHPNDLKES
                     NTLMNHVIYTIDSDYVEPIHRLDQETVGLLIVAKNPLMKKILDRMLEDNDITRIYKAN
                     VKALLPLKPQTIDMPIGKDKFHSNKRRVSPTGQRAITHILTSKMIKEAVCQLEIKLDT
                     GRTHQIRVHLAEIGHPVIGDPLYGDSTLRQLELESYKIEFVHPLTKEVISVSLDD"
     gene            3291..4403
                     /locus_tag="NBI57_002151"
     CDS             3291..4403
                     /locus_tag="NBI57_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing sensor histidine kinase"
                     /protein_id="HCD1823081.1"
                     /translation="MEQRTRLALLKEIAEFLNEETEMYSMTQGALKYLIEGSNFTTGW
                     IFFINSVGEHELVSHVALPQSLTADHCHYIKDGSCWCVKAFNQRRLMKASNIVNCSRI
                     NLASKAFPSQNDNITHHATVPLKSGQEQFGILNVASPNTEIYSDEDLELLESVAFQLG
                     SAIKRIYLTDREKEAAKINERNRLARDLHDSVNQMLFSVKLTAHAAYGMSNESIAKQA
                     FKTIEKTSQNAVNEMRALIWQLKPVGLEQGLIHALTAYSKLMHIQLNVNVEGLIDLSN
                     EIEENIYRALQECINNVKKHADTNKMDLTLKQMNDILYIDVIDYGQGFEIDNVQIASS
                     HGINNIKQRVKLLRGKVTFHSQPTKGTQIQFTIPIK"
     gene            4425..>5029
                     /locus_tag="NBI57_002152"
     CDS             4425..>5029
                     /locus_tag="NBI57_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCD1823082.1"
                     /translation="MNKVILVDDHYIVRQGLRFLLSTIENIEVLQDFADGETFLEYLK
                     EHEHPDIVLLDLVMPGMNGIEITEYIKAHYPEIKVLVLTSYVDDEHVISAINKGADGY
                     EMKDVEPQQLIETIRRVMNGEKMIHPKAQDVFETVSQKPHYTNKLSKREIEVLREMVK
                     GKTNKEIAETLFVSEKTIKTHVSHIFSKLQVSDRTQAAIYA"
BASE COUNT         1822 a          742 c          888 g         1577 t
ORIGIN      
        1 ttacaactca atttatcaaa aggattaatc gatgatgtta atgcgcaaac attcattaaa
       61 agtaatcaat caggtattgt aataagtgta atttgttgtt tattaataac aggaatattg
      121 tattatacac ttgatgggtt ttatgcgagt ttaacaggag ttttaggtac gcttgttgct
      181 ctaaatccat atacgacaat tgttagtgct gtattatata tacttacagc tattgctacg
      241 gttgaagcta ctaaaacaaa gtatcgtaat taaacttagt tcaacaataa ctaaataatg
      301 atagataaca cactaatcgt atgaccgtat cactgtaata aagggtgagt acgtgcgatt
      361 agtgtatttt tatttcaaat tatgaaaacg ttttaatcat aaattgctca attaaattat
      421 ttaatgatat actacagtta aataaacagt gataagggga gaaattgaat gtcagtaaga
      481 attgaacatg atacttttgg agaaatagaa gtacctgcag ataaatattg gggtgctcaa
      541 acagaaagaa gtaaacgcaa tttcccagtt ggtaaagagc gtatgccaat cgaagtagtt
      601 tatggttttg cacaactaaa gcgtgcagca gcattagcta attttgattt aggaaaatta
      661 agcgaggcaa agaaagatgc cattgtatac gcatgtgatc aaattttatc aggtgaatta
      721 gatgaacact tcccactagt tgtatggcaa acaggaagcg gtacacaaag taatatgaat
      781 gtgaacgaag tagtaagtta tgttgctaat atgtatttaa aagatcatca aattgatgaa
      841 agtatccacc caaatgatga tgtaaatgaa tcacaaagtt cgaatgatac attcccaact
      901 gctatgcacg ttgcattata tcaagaggtt gaaacaaaat tagaacctgc attaaaactt
      961 ttaagaaata ctttgaaaga aaaagaagac aaatttgaat caattattaa aattggtcgt
     1021 acacatttac aagatgcaac gccgatcaaa ctaggacaag agattagtgg ctggcgttat
     1081 atgcttgacc gttgcgaaat aatgttatct gaatctaaga agcacatttt aaatcttgcc
     1141 atcggtggta cggctgttgg tactggtatt aatgcgcatc ctgaatttgg tgataaggtg
     1201 gcacattata tttcagaaaa tacgggttat ccatttgtat cttctgaaaa taaattccac
     1261 gcacttacag cgcatgatga agttgttcaa ttgcacggaa cattgaaggc attagctgga
     1321 gatttaatga aaattgctaa tgatgtgaga tggttggctt caggtccacg agctggtttg
     1381 gcagaaattt ccatccctga aaatgaacca ggttcatcaa ttatgcctgg taaagttaat
     1441 cctacacaat gtgaaatgtt aacaatggtt gcagtccaag taatgggtaa tgatacagtt
     1501 gttggcttcg caagttcaca aggtaacttt gaattgaatg tttataaacc agttattatg
     1561 cataatacac tacaatcaat ttacctttta gctgatggta tggaaacatt taataacaat
     1621 tgtgcagtgg gcattgagcc aatcgaagag aatattgata attatttaaa tcaatcatta
     1681 atgttagtta ctgcattaaa tccacatatt ggttatgaaa aagcagctca aattgctaag
     1741 aaagcccata aagaaggttt aactttaaaa gaatctgcaa ttcaaactgg atatgttaca
     1801 gaagaacaat ttgaagcatg gattaaacca gaagatatgg tagatcctca ttaattaaat
     1861 attaatataa aataaagttt gggcattata gtgttcaagc tttatttttt gtctatatca
     1921 aattgaactt ataagtccga tttgatatag agccatgaaa aggaaaaact tttcatggag
     1981 tccgttttat tatagaacct attttttata tgatgaattg ttgtacatgt attggccaaa
     2041 caacctgtga taaaaatctt atgatgtcat gtattgtcca tagatacacg taacgcagtt
     2101 gaaaaagact acaagtcatt atccaaggat gttataaaat gaataattgc taaaatatca
     2161 aaatataaaa tcctaagcat cgtaaaaaac aaaaaaaccg aacatctgaa aaaattaata
     2221 attttccatc tatccgattt aagttgatac ttgatttagt tacaatgcgg tattgcatgt
     2281 ttaaatatca tgcaaaacta atcaaattag tcatccaaag aaacggaaat gacttcctta
     2341 gtcaagggat gcacaaactc tattttgtaa ctttcaagtt ctaattgtct taacgttgaa
     2401 tcaccatata aaggatcacc aataacaggg tgaccaattt cagctaaatg cacacgaatt
     2461 tgatgagtac gtccagtatc caacttgatt tcaagttggc acacagcttc ttttatcatt
     2521 tttgaagtta aaatgtgtgt aattgcacgc tgtccagtag gagacacacg tcgtttattc
     2581 gaatggaatt tatctttacc aattggcata tcaatcgttt gtggttttaa aggtaaaagt
     2641 gccttaacat ttgctttgta tatccgcgta atatcattgt cttctaacat gcgatcaaga
     2701 atttttttca ttaaaggatt tttagcaaca attaataatc ctactgtttc ctggtccagt
     2761 cgatgaattg gttcgacata gtcactatca atagtgtaaa tcacatgatt cattaaagta
     2821 ttgctttctt ttaaatcatt agggtgagtc ttaacacctt taggtttcat tacgattgcc
     2881 atatcatcgt cttcgtattt aatttgtgca taacgataac ttggaacata attacttttt
     2941 tcatcgatgg ttggaacaaa tacatcgtcg ccagtatgca ctttatccat taatcgcgca
     3001 ggtttatcat taatagtaat atctttagac atatttaaat gatgtaaatc ttttttaggt
     3061 accttaagtt gttggaaaat atctcgtaaa cttaagtcat taaagttttc tggtatttta
     3121 aatttcataa aatcctccta tagtctttaa tatcatatca caaatcagtt ctcctaaata
     3181 taactattga gtaatcaagg ccattttatc atgttcacgt ctaagattta aaaatataat
     3241 aactcaatgt ataatagtta atacacgtaa aaatgaattg gagcgatttg atggaacaaa
     3301 ggacgcgact agctttatta aaagagattg ccgaattttt aaacgaagaa actgaaatgt
     3361 atagcatgac ccaaggtgcg ttaaaatatt taattgaggg cagtaatttc acgacaggct
     3421 ggatattttt catcaatagc gtaggtgaac atgaacttgt atcacatgtg gctctaccac
     3481 aatctttgac agcagaccac tgtcactata ttaaagatgg gtcttgttgg tgtgtgaaag
     3541 catttaatca acgtaggcta atgaaagcgt cgaatatcgt caattgttct cgtattaact
     3601 tagcctctaa agcatttcct agccaaaatg acaatattac acatcatgca acggtaccac
     3661 ttaaatcggg tcaagaacaa ttcggcattt taaatgtagc gtccccaaat actgaaatat
     3721 atagtgatga agatttagag ttgctagagt ctgtcgcatt tcaattaggg tcagccatca
     3781 aacgtattta tttaacagac cgtgaaaaag aagcagcaaa aattaatgaa cgtaatcgtt
     3841 tagctagaga tttacatgat tcagtaaatc aaatgttatt ttctgtaaaa ctaacagcac
     3901 atgctgcgta tggcatgtca aacgaatcca tcgctaaaca agcattcaag acgattgaga
     3961 aaacgagtca aaatgccgtt aatgaaatgc gtgcattgat ttggcaactt aagccagttg
     4021 gacttgagca aggtttaatt catgctttga cagcatatag taaattaatg catatacaat
     4081 taaatgtaaa tgtagaaggt ttaatcgatt tatctaatga aatcgaagaa aacatatata
     4141 gagcattaca agagtgtatt aataatgtta agaaacatgc tgatacgaat aaaatggatc
     4201 ttacattaaa acaaatgaac gatatactat atattgacgt gattgattat ggtcaaggat
     4261 ttgagatcga taatgttcag atagcttcat cacacggtat taataacata aaacaaagag
     4321 ttaaattatt aagaggtaaa gtgacctttc attctcagcc aacaaagggt acacaaatac
     4381 aattcacaat tcctataaaa tagcttgatt taaggagata acccatgaac aaagtaatat
     4441 tagtagatga ccattatatt gtgcgacaag gattgcgatt tttattatcc acgattgaaa
     4501 acatagaagt tttacaagac tttgcagatg gagaaacatt tttagaatat ttaaaagagc
     4561 atgagcaccc tgatattgtg ctattagatt tagtgatgcc tggcatgaat ggtattgaaa
     4621 ttacggaata tattaaggca cattatccgg aaattaaagt tttggtatta acaagttatg
     4681 ttgatgatga acatgtaatt tcagcaatca ataaaggtgc tgatggttat gaaatgaaag
     4741 acgttgagcc tcagcaatta attgaaacta ttagacgagt tatgaacggt gaaaaaatga
     4801 tacatcctaa ggcacaagat gtattcgaaa cagttagcca aaaaccacac tacacgaata
     4861 agttgtcaaa gagagaaatt gaagtgttac gtgaaatggt taaaggtaaa acaaataaag
     4921 agattgcaga aactttattt gtatctgaaa aaacaattaa aacacatgtc agtcatatat
     4981 ttagtaaatt acaagttagc gatcgtacac aagcagcaat ttatgcaat
//
LOCUS       DAHHSC010000171         4965 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.172, whole genome shotgun sequence.
ACCESSION   DAHHSC010000171 DAHHSC010000000
VERSION     DAHHSC010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 4965)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4965)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4965
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.172"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            89..1144
                     /locus_tag="NBI57_002153"
     CDS             89..1144
                     /locus_tag="NBI57_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000145567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HCD1823083.1"
                     /translation="MTGFSVYLGQPLDEAYIKRMIKQGYQMIFTSVQIPEEDDETKYH
                     YFTKLLNLLKYEQVTYLIDANPSILTPSFYEHLRQYDAQFMIRIDHSTSIEAIEAIMA
                     QGLKCCLNASIISRELLTSLHQQLNDFTLLSFCHNYYPRPDTGLSVDLVNKKNELIYQ
                     FNPKAQIYGFIVGSGLRGPLHKGLPTIEATRHSHPVVAAKLLQETGVSEVLVGDSLIE
                     MRQAKQLIDFCKHRHFTLCIEEVFDTTVTYLFDMCHKVRPDNPENVIRSETSRQICPH
                     SIQPQFTTQRRIGSVTVDNLNNGRYQGEMQIVRQTLSAHNNVNVVAQIIKEDLPLLSC
                     IEPNDTFDFQKTRECKK"
     gene            1144..2040
                     /gene="murQ"
                     /locus_tag="NBI57_002154"
     CDS             1144..2040
                     /gene="murQ"
                     /locus_tag="NBI57_002154"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HCD1823084.1"
                     /translation="MENSTTEARNEATMHLDEMTVEEALITMNKEDQQVPLAVRKAIP
                     QLTKVIKKTIAQYKKGGRLIYIGAGTSGRLGVLDAAECVPTFNTDPHEIIGIIAGGQH
                     AMTMAVEGAEDHKKLAEEDLKNIDLTSKDVVIGIAASGKTPYVIGGLTFANTIGATTV
                     SISCNEHAVISEIAQYPVEVKVGPEVLTGSTRLKSGTAQKLILNMISTITMVGVGKVY
                     DNLMIDVKATNQKLIDRSVRIIQEICAITYDEAMALYQVSEHDVKVATVMGMCGISKE
                     EATRRLLNNGDIVKRAIRDRQP"
     gene            2052..3506
                     /locus_tag="NBI57_002155"
     CDS             2052..3506
                     /locus_tag="NBI57_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HCD1823085.1"
                     /translation="MTKEQQLAERIIAAVGGMDNIDSVMNCMTRVRIKVLDENKVDDQ
                     ELRHIDGVMGVIHDERIQVVVGPGTVNKVANHMAELSGVKLGDPIPHHHNDSEKMDYK
                     SYAADKAKANKEAHKAKQKNGKLNKVLKSIANIFIPLIPAFIGAGLIGGIAAVLSNLM
                     VAGYISGAWITQLITVFNVIKDGMLAYLAIFTGINAAKEFGATPGLGGVIGGTTLLTG
                     IAGKNILMNVFTGEPLQPGQGGIIGVIFAVWILSIVEKRLHKIVPNAIDIIVTPTIAL
                     LIVGLLTIFIFMPLAGFVSDSLVSVVNGIISIGGVFSGFIIGASFLPLVMLGLHHIFT
                     PIHIEMINQSGATYLLPIAAMAGAGQVGAALALWVRCKRNTTLRNTLKGALPVGFLGI
                     GEPLIYGVTLPLGRPFLTACIGGGIGGAVIGGIGHIGAKAIGPSGVSLLPLISDNMYL
                     GYIAGLLAAYAGGFVCTYLFGTTKAMRQTDLLGD"
     gene            3509..4384
                     /locus_tag="NBI57_002156"
     CDS             3509..4384
                     /locus_tag="NBI57_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HCD1823086.1"
                     /translation="MTNILYRIDKQLSDFTKTEKIIADYILKNPHKIIDMTVNDLADV
                     TNVSTASIVRFSRKMTHQGFQELKIAISRYLPEDIATNPHLELIENESVETLKNKMIA
                     RATNTMRFVATNIMDAQIDAICDVLKNARTIFLFGFGASSLTIGDLFQKLSRIGLNVR
                     LLHETHLLVSTFATHDDRDCMIFVTNQGSHSELQSIAQVATHYSIPIITISSTANNPV
                     AQIADYALIYGRTDENEMRMAATTSLFAQLFTVDILYYRFVALNYHAILDCITQSKMA
                     LDNYRKHLATIDFKH"
     gene            complement(4578..4859)
                     /locus_tag="NBI57_002157"
     CDS             complement(4578..4859)
                     /locus_tag="NBI57_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1823087.1"
                     /translation="MVLITYQIILFFIISLSYYLTLNHYMAVTVGNFTSIFGMFAAIL
                     FMYYYLLYKSPEYNQRKRFKHFIHITNLIIIAFSTFVLVHLALKLFFSI"
BASE COUNT         1670 a          729 c         1035 g         1531 t
ORIGIN      
        1 gtaatgtgtt ttctcttata gtaaaatgta agcgattaca caactcaact atattttcct
       61 aataattata ttgataagga gggctacttt gacaggcttt tcagtgtatt taggacaacc
      121 tttagatgaa gcgtatatta agcgaatgat taaacaaggt taccaaatga tttttacatc
      181 tgtacaaata ccagaagaag atgacgagac aaaatatcat tatttcacaa aactactcaa
      241 tttattaaaa tatgaacaag tgacttacct catagatgct aatccatcta ttttaacacc
      301 atctttttat gagcatcttc gacaatatga tgcacaattt atgattcgta tcgatcatag
      361 tacatcaatt gaggcaatcg aagcgataat ggcacagggt ttaaagtgct gtttgaatgc
      421 aagtattatt tcccgggaat tgttaacaag cttacatcaa caattgaatg attttacatt
      481 actttcattt tgtcataact attatccaag accagatacg ggattatctg ttgacttggt
      541 caataagaaa aatgaactca tttatcaatt taatccaaag gcacaaatat atggttttat
      601 tgtagggagt ggtttgcgag gtcctttgca taaaggcttg ccaacaattg aagcaacgag
      661 acatagtcat cctgtcgttg cagctaaatt attacaagaa actggtgtat ctgaagtgtt
      721 agttggagac tcattgattg aaatgaggca ggcaaaacaa cttatagatt tttgcaagca
      781 taggcatttc acgttatgta ttgaagaagt gtttgatacg acagtgactt accttttcga
      841 tatgtgtcat aaagtacgcc cggataatcc ggaaaatgtc attcgttcgg aaacgtcaag
      901 acaaatatgt ccacattcga ttcaaccaca gtttacgacg caacgacgca ttggttcagt
      961 aaccgttgat aatttgaata acggacgtta tcaaggcgaa atgcaaattg tgagacaaac
     1021 gcttagtgca cataacaatg tgaatgttgt tgcacaaatt attaaagaag acttaccact
     1081 gttaagttgt atcgagccga atgatacatt tgattttcaa aaaactaggg agtgtaagaa
     1141 gtgatggaaa atagtacgac cgaagcgcgt aatgaagcga cgatgcatct tgatgaaatg
     1201 actgtggaag aggctttaat tacgatgaat aaagaagatc agcaagtccc gttagcagtt
     1261 cgaaaggcaa taccacaatt gacaaaagta ataaaaaaaa caattgcaca gtataaaaag
     1321 ggtggacgat tgatctatat cggtgcaggt acaagtggaa gattgggtgt cttagatgca
     1381 gcggagtgtg tacctacatt caatactgac cctcatgaaa ttataggtat tattgctggt
     1441 ggacaacatg ctatgacgat ggctgtagaa ggtgcggaag atcacaaaaa attagcggaa
     1501 gaagatttga aaaatataga tttaacatca aaagatgtcg ttataggaat tgccgcgagt
     1561 ggcaaaacgc catatgttat aggcggttta acatttgcta acacaatcgg tgctacaaca
     1621 gtatctattt catgcaatga acatgcagtt ataagtgaaa ttgcgcagta tccagtagaa
     1681 gttaaagttg gtccagaagt attaactggt tcaacacgtt taaagtctgg tacagcacaa
     1741 aagttaattt taaatatgat ttcaaccatc acaatggttg gtgtcggaaa agtttacgat
     1801 aacctcatga ttgatgttaa agcaaccaat caaaaactga tcgaccgttc agtgcgtatt
     1861 attcaagaaa tatgtgctat cacatatgat gaagcaatgg cgttatatca ggtatctgag
     1921 catgatgtga aagttgcgac agttatgggt atgtgtggca tttctaagga agaagcaaca
     1981 agacggttat taaacaatgg tgacattgtt aaacgagcaa tcagagatag acaaccttag
     2041 gagggattta aatgaccaaa gaacaacaac ttgcagaacg aattattgct gcagtaggtg
     2101 gtatggataa tatagatagt gtcatgaact gcatgacacg tgtgcgtatt aaagtattag
     2161 atgaaaataa agtagatgac caagaactaa ggcatattga tggtgtcatg ggtgttatac
     2221 acgatgaacg cattcaagtt gtggttggac ctggtacagt caataaagtg gctaatcata
     2281 tggcggaatt aagtggtgtt aaactaggtg acccaatacc acaccatcac aatgatagtg
     2341 aaaaaatgga ctataaatca tatgcagctg ataaagcaaa ggcgaataaa gaagcacata
     2401 aagcaaaaca aaagaatggt aagttgaata aagtattgaa atcaattgcc aatatcttta
     2461 taccgttgat tcctgcattt attggagctg gattaattgg tggtattgca gcagtactga
     2521 gtaacttaat ggtggcaggc tatatttcag gtgcttggat tacgcaactt ataacagtat
     2581 tcaatgtcat taaagacggt atgttagcat acttagctat ttttactggt attaatgcgg
     2641 ctaaagaatt tggtgcaaca ccaggacttg gtggcgtgat tggtggtaca acgttattaa
     2701 cgggtattgc tggtaaaaat attttaatga atgtcttcac tggagaacca ttgcaacctg
     2761 gacaaggtgg aattattggc gttatttttg ccgtttggat tttaagtatt gtcgaaaaga
     2821 gattgcataa aattgtgcca aatgcgattg atattattgt aacgccgact attgcattgt
     2881 tgattgtagg actattaact atctttatct ttatgccatt agcaggtttt gtttcagaca
     2941 gtttagtttc agtagttaac ggaattatta gtattggtgg cgtatttagt ggatttatca
     3001 ttggagcaag cttcctaccg ttagttatgt tagggcttca tcatattttt acgccaattc
     3061 atatagaaat gattaaccaa tctggtgcta cttacttatt gccaattgca gcgatggctg
     3121 gtgctggaca agtaggtgcc gcattagcac tttgggtaag atgtaaacgc aacacaacat
     3181 tacgtaatac tttaaaaggt gcattgccag ttggtttcct aggtatcgga gaaccattaa
     3241 tctatggtgt gactttgcca ttaggtcgac ctttcttaac tgcttgtatt ggtggtggta
     3301 ttggtggcgc tgtaataggt ggaattggac atattggtgc caaagcaata ggcccaagtg
     3361 gtgtgtcact cttaccatta atctcagata atatgtattt aggttatatt gcaggattac
     3421 ttgctgcata tgctggtgga ttcgtttgta catatttatt tggaacgaca aaggcgatgc
     3481 gacagacaga tttgttgggt gattaatgat gacaaatatt ttatatcgca ttgataaaca
     3541 gttgagtgat tttacgaaga cagaaaagat aatcgctgat tacattttaa agaatccaca
     3601 taaaatcatt gatatgacgg tgaatgattt ggcagatgtt acgaatgtta gtacagcatc
     3661 aattgttaga tttagtcgga aaatgacaca tcaaggtttt caagagctaa agattgcgat
     3721 atctcgatac ttacccgaag atattgcaac caatccacat ttagaattga ttgaaaatga
     3781 atctgtagaa actttgaaaa ataaaatgat tgctagagca acgaatacga tgcgatttgt
     3841 agctactaat attatggatg cgcaaattga tgcaatttgt gatgtgttga aaaatgccag
     3901 gacaatattt ttatttggat ttggcgcatc gagtttgact attggtgatc tttttcaaaa
     3961 gttatctcgt attggcttaa atgtcaggtt attacatgaa acgcatttac ttgtgtcaac
     4021 atttgcgacg catgatgata gagattgcat gatttttgtg acgaatcaag gtagtcatag
     4081 tgaattgcag tcaattgcac aggtggccac acattacagt attcccatca taactatatc
     4141 tagtacagct aataatccag tggctcaaat tgcagactat gcattgattt atggcagaac
     4201 tgatgaaaat gaaatgcgta tggcggctac aacttcacta tttgcacagt tattcacggt
     4261 agatatattg tactatcgat ttgtagcatt aaattatcat gcgattctag attgtataac
     4321 ccaatcgaaa atggcacttg ataattaccg gaagcatctt gcgacgatag attttaaaca
     4381 ttagatattg tgagcgttct aatataaagt tagttgcaac gtaaacaatt gaagtttttg
     4441 atgtgattca gttgtttgat gaaaaattgt tgctacaagt ttgtaaaact ggcatagagg
     4501 ctattttgaa aatgataaag gcgtgacaaa ttaaagcaaa gttcaggttt gagctttgct
     4561 tttttcatag atggaaatta aatgctgaag aataatttta atgctaaatg aactaatacg
     4621 aaggtgctaa aagcaattat tatcaaatta gtgatatgaa tgaaatgttt aaatcgtttg
     4681 cgttgattgt attcgggact tttatagagt aggtagtagt acataaagag tatggctgcg
     4741 aacatgccga atattgaagt gaagttacct acagtgactg ccatgtaatg atttaaagtt
     4801 aaatagtaac ttagactaat aataaaaaat aaaatgattt gataggtaat taacaccatt
     4861 caagtccctc cattaatcgt agacaaaaaa tttatcaatg atttgattat ttgtattcaa
     4921 attttagtat acgacttacc tcaaagacaa atattaaaag ataag
//
LOCUS       DAHHSC010000172         4919 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.173, whole genome shotgun sequence.
ACCESSION   DAHHSC010000172 DAHHSC010000000
VERSION     DAHHSC010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 4919)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4919)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4919
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.173"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            315..740
                     /locus_tag="NBI57_002158"
     CDS             315..740
                     /locus_tag="NBI57_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000769702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAP domain-containing protein"
                     /protein_id="HCD1823088.1"
                     /translation="MKLKSFVTATLALGLLSTVGAALPSHEASADSNNGYKELTMDGK
                     HTVPYTISVDGITALHRTYFVFPENKKVLYQEIDSKVKNELASQRGVTTEKINNAQTA
                     TYTLTLNDGNKKVVNLKKNDDAKNSIDPSTIKQIQIVVK"
     gene            complement(1124..1828)
                     /gene="budA"
                     /locus_tag="NBI57_002159"
     CDS             complement(1124..1828)
                     /gene="budA"
                     /locus_tag="NBI57_002159"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HCD1823089.1"
                     /translation="MTNVLYQHGTLGTLMAGLLKGTASINELLQHGDLGIATLTGSNG
                     EVIFLDGKAYHANEHKEFVELKGDELTPYATVTKFVADTSYETKDKSSEAVFAEIKEK
                     MLSENLFSAVKISGLFKKMHVRMMPAQEPPYTRLIDSARRQPEQTETYVKGSVVGFFT
                     PELFHGIGSAGFHVHFANDDRNFGGHVLDFEVEDVKVEIQNIETFEQHFPIQDKDFTK
                     ANIDYKDIADEIREAE"
     gene            complement(1865..3529)
                     /gene="alsS"
                     /locus_tag="NBI57_002160"
     CDS             complement(1865..3529)
                     /gene="alsS"
                     /locus_tag="NBI57_002160"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase AlsS"
                     /protein_id="HCD1823090.1"
                     /translation="MTDKKYTAADMVIDTLKNNGVEYVFGIPGAKIDYLFNALIDDGP
                     ELIVTRHEQNAAMMAQGIGRLTGKPGVVLVTSGPGVSNLTTGLLTATSEGDPVLALGG
                     QVKRNDLLRLTHQSIDNAALLKYSSKYSEEVQDPESLSEVMTNAIRIATSGKSGASFI
                     SIPQDVISSPVESKAISLCQKPNLGVPSEQDINDVIEAIKNASFPVLLAGMRSSSAEE
                     TNAIRKLVERTNLPVVETFQGAGVISRELENHFFGRVGLFRNQVGDELLRKSDLVVTI
                     GYDPIEYEASNWNKELETQIINIDEVQAEITNYMQPKKELIGNIAKTIEMISEKVDEP
                     FINQQHLDELEQLRTHIDEETGIKATHEEGILHPVEIIESMQKVLTDDTTVTVDVGSH
                     YIWMARNFRSYNPRHLLFSNGMQTLGVALPWAISAALVRPNTQVVSVAGDGGFLFSSQ
                     DLETAVRKNLNIIQLIWNDGKYNMVEFQEEMKYKRSSGVDFGPVDFVKYAESFGAKGL
                     RVTNQEELEAAIKEGYETDGPVLIDIPVNYKDNIKLSTNMLPDVFN"
     gene            complement(4023..4205)
                     /locus_tag="NBI57_002161"
     CDS             complement(4023..4205)
                     /locus_tag="NBI57_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1823091.1"
                     /translation="MKDSIFWKKAFIPVYFIVAMLVFLLFRFYIKTDNFSIYLMIIFL
                     ICLGTASIIYNYKNNR"
     gene            complement(4779..>4919)
                     /locus_tag="NBI57_002162"
     CDS             complement(4779..>4919)
                     /locus_tag="NBI57_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000411031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-directed DNA polymerase"
                     /protein_id="HCD1823092.1"
                     /translation="SFIKMIDFKKGVSLQKKFNEVLGNIESSNMYRDNIDFDDIELHW
                     IN"
BASE COUNT         1623 a          849 c          707 g         1740 t
ORIGIN      
        1 aataatagac gctttgacat actcattccc ccataatatt ctaatgaatt tatctataac
       61 ttaaatacac tataagtctc aattaaaagt ataagatcga ctatatgctt ttcatgctta
      121 ttatatacaa ctaatgaatc cctataaact aaaaacattg cttaccactt ttcaaaatac
      181 acgggtcata tccctcgtta aaaactatta ctaaaaatca aaaatataaa aaaatcaaaa
      241 agtatgtttt aaactttatg aaacgtgtta tataaatagt gtaatatacc acaactttaa
      301 aaggagattg agatatgaaa ctaaaatcat ttgttactgc cactttagca ttgggattat
      361 tatcaacagt aggtgcagct ttaccgagtc atgaagcatc tgcagatagt aataacggtt
      421 ataaggaatt gacaatggat ggaaaacaca ctgtgcctta cacgatctca gtagatggta
      481 ttactgcatt acatcgaact tactttgttt ttccagaaaa taaaaaggta ctttatcaag
      541 aaattgacag taaagtaaaa aatgaattag cttcacaacg tggtgttaca acagaaaaaa
      601 ttaataatgc acaaacagca acgtatacac ttactttgaa tgatggtaat aaaaaagtag
      661 tgaatctaaa gaaaaatgac gacgctaaaa attcaattga tccaagtaca atcaaacaaa
      721 tacaaattgt agttaaataa taatttgaaa atgaaaatag ttacacaggg caaacgctga
      781 aaaattgcgt ttgtcttttt agtatgaaat gaatgatcga acttataatt tcttattgat
      841 tgcttctact gacaatagat tagatcggcc tatactaaag taaattctat attaatgtct
      901 tccgcaattt ataatctaac ataacagcaa atctcaaata tctggaacct aataaatccc
      961 ctcactactg ctaatagtga agggatttat tttattactt agacgtaatt ataaatatat
     1021 ttgttctatt gtagtcatgg ttaatcatat ttacaaacta tcatttctaa atcgcgcatt
     1081 atgaagtcaa ttttcataaa ccgtcattat atttctggat tcattattca gcttctctaa
     1141 tttcgtctgc aatatcttta tagtcaatat ttgctttagt gaaatcttta tcttgaattg
     1201 gaaaatgctg ttcaaatgtt tctatatttt ggatttctac tttaacatct tctacttcaa
     1261 aatctaagac atgtccacca aagttacgat catcattcgc aaagtgtaca tgaaatcctg
     1321 ctgatccgat accatggaat aattctggtg taaagaaacc aacgactgaa cctttgacat
     1381 acgtttcagt ttgttcaggt tgtcttctag ctgaatcgat taaacgtgta taaggtggtt
     1441 cttgagccgg catcatacgt acatgcattt ttttaaataa gcctgaaatt tttactgctg
     1501 aaaataaatt ttcactcaac atcttttcct taatttctgc aaaaactgct tctgaagatt
     1561 tatctttcgt ttcatagctt gtatctgcta caaatttagt tacagttgca tatggtgtta
     1621 actcgtcacc ttttaattct acaaattctt tatgttcatt tgcatggtaa gcttttccat
     1681 ctaaaaagat tacctcaccg tttgaacctg ttagtgtagc gatacctaag tcaccatgtt
     1741 gcaataattc atttattgat gcagttcctt ttaataatcc tgccattaat gtgcctaatg
     1801 taccatgttg gtataaaaca ttagtcataa aatctcccac tcctctttaa catttatctt
     1861 tattttagtt aaatacgtca ggtaacatat ttgttgaaag tttgatatta tctttgtaat
     1921 ttacaggtat atcaattaat actggaccat ctgtttcata gccctcttta attgccgctt
     1981 ctaattcttc ttgattagta actcgtaaac ctttcgcgcc aaatgattct gcatatttta
     2041 caaaatctac aggaccgaag tctacacctg acgaacgttt atatttcatt tcttcttgga
     2101 attcaaccat gttatatttt ccatcattcc aaataagctg gatgatattt aaatttttac
     2161 gtacggccgt ttctaaatct tgtgatgaaa ataaaaagcc accatcgcca gcaacggaca
     2221 caacttgcgt attagggcgc acaagtgcag ctgaaattgc ccacggtaat gctacaccaa
     2281 gcgtttgcat accattgcta aataataaat gtcttggatt gtaacttctg aaattacgtg
     2341 ccatccaaat atagtgactt ccaacatcaa ctgttacagt agtatcatca gttaatacct
     2401 tttgcataga ttcaataatt tccactggat gtagaattcc ttcttcatgc gtcgctttaa
     2461 taccagtttc ttcatcaata tgtgttctta attgttctaa ttcgtctaaa tgttgttgat
     2521 ttataaatgg ctcatccact ttttcagaaa tcatttcaat cgttttagca atattaccaa
     2581 tcaactcttt tttcggttgc atataattag taatttcagc ttgaacttcg tcaatattga
     2641 taatttgtgt ttctaattct ttattccagt tactagcttc gtattcaatt ggatcataac
     2701 cgattgtaac aactaaatca cttttacgta ataattcatc accaacttga ttgcggaata
     2761 agcccacacg accgaagaaa tgattttcta attcacgact aattacacct gcaccttgga
     2821 atgtttctac aactggtaaa ttcgtgcgct caactaattt gcgaatggca tttgtttctt
     2881 ctgcacttga acttctcata ccagctaata aaacaggaaa tgatgcattt ttaatcgctt
     2941 caatgacatc attaatatct tgttcactcg gtactcctaa atttggtttt tggcaaagtg
     3001 atatagcttt agattcaact ggtgaagaaa taacgtcttg cggaatacta ataaaacttg
     3061 cgccactttt tcctgaagta gcaattcgaa ttgcatttgt cataacttct gataatgatt
     3121 caggatcttg tacttcttca ctgtattttg atgaatattt taatagcgca gcattatcaa
     3181 tactttgatg cgttaatcgt aataaatcat tacgtttcac ttggccacct aacgctaata
     3241 caggatcccc ttcagatgta gctgttaata gtccagtcgt taaattactt acaccagggc
     3301 cacttgtaac aagtactaca cccggtttac ctgttaatct tccaatacct tgtgccatca
     3361 ttgcagcatt ttgttcatga cgagtgacaa taagttcagg accatcatca attaaagcat
     3421 taaatagata gtctatcttt gcacccggaa taccaaaaac atattctacc ccattatttt
     3481 tcaaagtatc aataaccata tcggctgcag tgtacttttt atcagtcatt tatattcatt
     3541 tccttccaat tgtgatgtat agatgtgtat tacatgtaat tacattgtga tttgtaatct
     3601 aatgatagtt acgcaaaatt aatgatgttt atcaaaattt tacatgtacg attagatttg
     3661 taatacataa aaattgcctt cattacatta tataaccttt gttagatgaa taaaaatcgt
     3721 tatttgtaat actaagtatt ccaaaaatgc atgagtagat tttgtgcgtt gtgattatga
     3781 gggatgttgg attgattgat gtataggatt gttacgatat tttgggatga ggtgtttagt
     3841 caatggcggt ttgaaattgt cgttttatgg tgggctgaaa atgctgtttt atgtcgggct
     3901 tgaatttgtc gttttatgga gatataaaaa tgctgtttta tggcggtttc aaaatggtgt
     3961 ttcgattaat aagccacagc aagacttaga tctaactatg acttatacat ttcacactta
     4021 tattagcgat tatttttata gttataaatg atagatgcag ttcctaaaca aattaagaag
     4081 ataatcatta agtatataga aaagttatct gttttaatat aaaacctaaa aagtagaaac
     4141 accagcatcg caacaataaa ataaacagga ataaaagctt tcttccaaaa tatagaatct
     4201 ttcaattagg tcactcctta ttttgataat actctttaat taaggctctt gagttatcgt
     4261 ccggtcgttc tttcccttga accactatga ttagaacaca atcgtctggt tatcgtccac
     4321 cctccgcaag ctaaatgaca atttaataat gtacactttt gattgtgtaa acatgtacaa
     4381 attatacgtg taaatcaata atccatagtt gaattaattc tattcctaag acattaaata
     4441 aaggaaagtc catttatact ctacacattt catattcccc acacaaacgc aaaaaaacac
     4501 ctcgacataa catcgaggtg ttttgtatat atcgtccgac aattaacgtt ttaagaaatg
     4561 aggtgaacga cgagctgctt taagacctgg tttactttag gatttcgtat agttgcatat
     4621 actgtcttaa ggcatgatat taaaggattt ctaatttttt tgatagtatt gggtttcctc
     4681 taatttaacc taggggtatt aaaatataat aaaaatctgc tggctggtgg taaatttcaa
     4741 aagaaatatt aactaaaata tattaattat taagcggttt aatttatcca atgtagttct
     4801 atatcatcaa agtcaatatt atctctgtac atgttagacg attctatatt acctagtact
     4861 tcattaaatt tcttttgtaa actaacgcct ttcttgaaat caatcatttt aataaaact
//
LOCUS       DAHHSC010000173         4870 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.174, whole genome shotgun sequence.
ACCESSION   DAHHSC010000173 DAHHSC010000000
VERSION     DAHHSC010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 4870)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4870)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4870
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.174"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            423..1226
                     /locus_tag="NBI57_002163"
     CDS             423..1226
                     /locus_tag="NBI57_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HCD1823093.1"
                     /translation="MKKIATATIATAGFATIAIASGNQAHASEQDNYGYNPNDPTSYS
                     YTYTIDAQGNYHYTWKGNWHPSQLNQDNGYYSYYYYNGYNNYNNYNNGYSYNNYSRYN
                     NYSNNNQSYNYNNYNSYNTNSYRTGGLGASYSTSSNNVQVTTTMAPSSNGRSISSGYT
                     SGRNLYTSGQCTYYVFDRVGGKIGSTWGNASNWANAAARAGYTVNNTPKAGAIMQTTQ
                     GAYGHVAYVESVNSNGSVRVSEMNYGYGPGVVTSRTISASQAAGYNFIH"
     gene            complement(1244..1486)
                     /locus_tag="NBI57_002164"
     CDS             complement(1244..1486)
                     /locus_tag="NBI57_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1823094.1"
                     /translation="MTKLIENLKASIFVSTLTKNLSMYSRFDMPKFGCVNGCMLFILY
                     YRVNRIGENRNFEIFTNSIFFIEIKKANKALETLLA"
     gene            complement(1660..3060)
                     /gene="nhaC"
                     /locus_tag="NBI57_002165"
     CDS             complement(1660..3060)
                     /gene="nhaC"
                     /locus_tag="NBI57_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HCD1823095.1"
                     /translation="MMKRKPTFLESISTMIVMVIVVVTGFVFFDIPIQVLLIIASAYA
                     TWIAKRVGLTWQDLEKGIAERLNTAMPAILIILAVGIIVGSWMFSGTVPALIYYGLDL
                     LNPSYFLISAFFISAVTSVATGTAWGSASTAGIALISIGNQLGIPPGMAAGAIIAGAV
                     FGDKMSPLSDTTNLAALVTKVNIFKHIHSMMWTTIPASIIGLLVWFIAGFQFKGHSND
                     KQIQTLLSELAQIYQINIWVWVPLIVIIVCLLFKMATVPAMVISSFSAIIVGTFNHHF
                     KMTDGFKATFSGFNDSMIHQSHISSSVKSLLEQGGMMSMTQILVTIFCGYAFAGIVEK
                     AGCLEVLLTTISKGIHSVGSLICITVICCIALVFAAGVASIVIIMVGVLMKDLFEKYQ
                     VSRSVLSRTLEDSSTMVLPLIPWGTSGIYYTNQLHVSVGEFFMWTVPCYLCAIIAIIY
                     GFTGIGIKKSSNSRLT"
     gene            complement(3155..4237)
                     /locus_tag="NBI57_002166"
     CDS             complement(3155..4237)
                     /locus_tag="NBI57_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD/NADP octopine/nopaline dehydrogenase family
                     protein"
                     /protein_id="HCD1823096.1"
                     /translation="MKIAIVGSGNGAVTAAVDMVSKGHDVKLYCRNQSISKFQNAIEK
                     GGFDFNNEGDERFVKFTDISDDMEYVLKDAEIVQVIIPSSYIEYYADVMAEHVTDNQL
                     IFFNMAAAMGSIRFMNVLEDRHIETKPQLAEANTLTYGTRVDFENAAVDLSLNVRRIF
                     FSTYDRSCLNDCYDKVSSIYDHLVKEESLIKTNLENGNPEVHPGPTLLNVGRIDYAGE
                     FALYKEGITKHTVRLLHAIELERLNLGRRLGFELSTAKESRIERGYLERDKEDEPLNR
                     LFNTSPVFSQIPGPNHVESRYLTEDIAYGLVLWSSLGRVIDVPTPNIDAVIVIASTIL
                     ERDFFEEGLTVEEIGLDKLDLEKYLK"
     gene            4485..>4870
                     /locus_tag="NBI57_002167"
     CDS             4485..>4870
                     /locus_tag="NBI57_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4870 domain-containing protein"
                     /protein_id="HCD1823097.1"
                     /translation="MTTEMNDVTNHQPNRKSNDENIMAMLIYLLSLFTSIIGPLIIWL
                     LKKDESKLVDRAGKNYLNYTISYIIWSIVLVVITLIGVFLIATDISFIIIIGFIITFI
                     GILSIFAFSILSFVFTIIACVKYYNG"
BASE COUNT         1675 a          924 c          676 g         1595 t
ORIGIN      
        1 tccattaaat atacattttc atccaaatac caaagctttc ataatcatag aaacttgatc
       61 gttcgtgtcg aatttttatg cattttctga ttaaaagtcc tatattatgc acaaaatata
      121 catttagaat tttcagtaat attacaaatt tgtaacagac ttattttcga aacattcagt
      181 tttcacaaag aatgccgtag ttataagttt tgcaaaatgc aaatttctat acttttaaaa
      241 ttctattgtg tcaagatatt agcaaaaaat tacaaaacgt taacaaagca cacaaggacg
      301 ctaatttctg ttatagtctt tcttgtcgtt taattacgag agataactta taacattaat
      361 tttgtcacac ggacattacg aaaatatttt agatttaaaa tttttaggag gatattttta
      421 aaatgaagaa aatcgctaca gctactatcg caactgcagg attcgctaca atcgcaattg
      481 catcaggaaa tcaagctcat gcttctgagc aagataacta cggttataat ccaaacgacc
      541 caacatcata tagctatact tacactattg atgcacaagg taactaccat tacacatgga
      601 aaggtaactg gcatccaagt caattaaacc aagataatgg ctactacagc tattactact
      661 acaatggcta caataactac aacaattaca acaatggtta tagctacaat aattacagcc
      721 gttacaacaa ctactcaaat aataatcaat catataacta caataactat aatagttaca
      781 acacaaacag ctaccgtact ggtggtttag gtgcaagcta cagcacttca agcaacaatg
      841 ttcaagtaac tacaactatg gctccatcat caaatggccg ttcaatctca agtggttata
      901 cttcaggacg taacttatac acttctggtc aatgtacata ctacgtattt gatcgtgtag
      961 gtggtaaaat cggttcaact tggggcaatg caagtaactg ggctaacgca gctgcaagag
     1021 ctggttacac agtgaacaat acaccaaaag ctggtgcaat tatgcaaaca actcaaggtg
     1081 catacggtca cgttgcatac gttgaaagtg ttaacagcaa tggttcagta agagtttcag
     1141 aaatgaacta tggttatggc ccaggtgttg taacttcacg tacaatctca gctagccaag
     1201 ctgctggtta taacttcatt cactaattag tgatggctat gttttacgcc aacaaggttt
     1261 caagagcctt gttggctttt ttaatttcta tgaaaaaaat cgaattggta aaaatctcaa
     1321 aatttcgatt ttcaccaatc cgattcactc tgtaataaag aataaacaac atacagccat
     1381 tgacacaccc aaatttaggc atgtcaaatc ggctatacat tgataaattt ttagttaaag
     1441 tacttacaaa tatacttgcc ttcaaattct caattaattt agtcattaca aatcatgaca
     1501 taaaacttac attacatata tcaacatgtt ccgctacaaa acatattcag tatatgtata
     1561 gaggatattt attctaagtt aaaacctttt caaattataa ccgctacaat tataaatgat
     1621 taaagtgttt caacatatta tatatattcc acgctcacat taagttaaac gtgaattcga
     1681 tgacttttta atacctatcc ctgtaaaacc atagataatt gctataattg cgcataaata
     1741 acatggtact gtccacatga aaaattctcc aacagagaca tgaagttgat tcgtatagta
     1801 aatacctgat gttccccatg gtattaaagg taagaccatc gtacttgagt cttcaagtgt
     1861 tcttgataaa acggaacgag agacttggta tttttcgaac aaatctttca ttaacacacc
     1921 gaccataata attacaatcg aagcaacacc tgcagcgaat acaagcgcaa tacaacaaat
     1981 aacagtaata catattaaac ttcctacaga atggatgcct ttagaaatag tagttaataa
     2041 gacttctaaa catcctgctt tttctacaat acctgcaaat gcatatccgc aaaatatcgt
     2101 tactaatatt tgggtcatac tcatcatacc accctgttct aacaagcttt tcacactgga
     2161 tgaaatatga gactgatgta tcattgagtc gttaaaacca ctaaatgttg ctttgaaacc
     2221 atctgtcatt ttgaaatgat gattaaaagt ccccactata atggcagaaa agcttgatat
     2281 taccatagct ggcactgtag ccattttaaa tagcaaacaa acaatgatca caattaaggg
     2341 aacccagacc catatgttaa tttgataaat ctgtgcaagc tctgataaca aagtttgaat
     2401 ctgtttatca tttgaatgcc ctttaaattg aaatccagca ataaaccata ctaataaacc
     2461 tatgattgat gcaggtatcg tcgtccacat catcgaatgt atatgtttaa atatattaac
     2521 tttagtaaca agtgccgcta aattagttgt atctgataat ggtgacattt tatcgccaaa
     2581 cacagcccct gctataatag cacccgctgc catccctgga ggaatcccca attgattacc
     2641 aatagaaata agtgcaatcc ctgcagttga tgcagagccc catgctgtac cagttgctac
     2701 agatgtaaca gcacttataa aaaaggctga tattaaaaaa tagcttggat tcaataaatc
     2761 taagccataa taaatcaagg ctggcactgt gccagaaaac atccaactgc ctactataat
     2821 tcctaccgct agtataatta aaattgcagg cattgcagta tttaaacgtt ctgcaatgcc
     2881 tttttctaaa tcttgccatg ttaagcctac acgttttgca atccatgtgg catatgctga
     2941 ggcaataatt aataatactt gaattggaat atcaaaaaac acaaagcctg ttacaacaac
     3001 aataaccatt acaatcattg tcgaaattga ttctaaaaag gttggttttc ttttcatcat
     3061 ctatcgtccc cttcgcttat tgatgagaaa cctcttttct tcaatttatt gcttttgcat
     3121 aatgctatat ccttttctat cttcaagcca tcatttattt taaatatttt tctaaatcaa
     3181 gcttatctaa accaatttct tcaactgtta agccttcctc aaagaagtct ctctctaaaa
     3241 tggttgatgc aattacaatt actgcatcta tatttggtgt cggtacatca ataacacgac
     3301 ctaagcttga ccatagtact aaaccatatg caatatcttc agttaaatat ctgctttcta
     3361 catgatttgg tcctggaatt tgtgaaaata ctgggcttgt attaaacaaa cgatttaatg
     3421 gttcatcttc tttatcacgt tctaaataac cacgttcaat acgtgattct ttagctgttg
     3481 ataattcaaa acctaatcta cggcctaaat ttaaacgttc taattcgatt gcatgaagta
     3541 atctaactgt atgtttagta attccttctt tatataaagc gaactcgcca gcataatcaa
     3601 tgcgaccgac attcaataat gttggtcctg gatgcacttc aggattacca ttttcaagat
     3661 ttgttttaat taagctttcc tcttttacta aatgatcata aatacttgaa actttgtcat
     3721 aacaatcatt tagacagctt ctatcatatg ttgaaaagaa gatacgacgt acatttagag
     3781 ataaatcaac tgctgcattt tcaaaatcga cacgcgtacc atacgtcaac gtattagctt
     3841 ccgctagttg tggttttgtt tcaatatgtc tatcttctaa aacattcata aaacgaattg
     3901 accccattgc tgcagccatg ttgaagaata tcaactgatt atcagttaca tgctctgcca
     3961 ttacatcagc ataatactct atgtatgaag atggaataat cacttgaaca atttcagcat
     4021 cttttaaaac atattccata tcatcactaa tatcagtgaa ttttacgaaa cgttcatcac
     4081 cttcattatt aaaatcaaat ccgccctttt cgattgcgtt ttgaaactta cttatagatt
     4141 gattacgaca atataattta acatcgtggc ctttgctcac catatctact gctgccgtaa
     4201 ctgcgccatt tcctgatcct acaattgcta ttttcataaa caaccctctt tctatctttg
     4261 gttaaaatta aaaacaaatt atacgtaaga caatataaaa atgatgcgaa ttcattacgc
     4321 atcattataa atgttaagta tatataacct taaattaacc tatttaaacg ccgacgtcca
     4381 ccaattattt ttatttttaa taattgttta ataaacatgt ttatttttga ttatattttg
     4441 ttatagttaa agataatatt acaattttca aaggagtgtc taatatgaca actgaaatga
     4501 acgacgtaac aaatcatcaa ccgaatcgca aatccaatga tgagaacatt atggcgatgc
     4561 ttatctattt attaagttta ttcacatcta ttattggtcc actgataata tggttattga
     4621 aaaaagatga atcgaaacta gttgatcgtg ctggaaaaaa ttatcttaac tatacgattt
     4681 cttatattat ttggtctatc gttttagtcg ttatcacttt aataggcgtt ttcctaattg
     4741 ctaccgatat tagtttcatt atcattatag gtttcatcat tacatttatc ggtattttat
     4801 ccatattcgc tttctcaata ttatcttttg tattcacaat tattgcgtgt gtcaaatatt
     4861 ataatggcca
//
LOCUS       DAHHSC010000174         4859 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.175, whole genome shotgun sequence.
ACCESSION   DAHHSC010000174 DAHHSC010000000
VERSION     DAHHSC010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 4859)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4859)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4859
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.175"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            complement(171..692)
                     /locus_tag="NBI57_002168"
     CDS             complement(171..692)
                     /locus_tag="NBI57_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YwhD family protein"
                     /protein_id="HCD1823098.1"
                     /translation="MEVISLSEKKGFNFNIIKNDPLDGHKGTNIGSISLDNIAPVFID
                     VANKEAFIDIGGMHARAKVEKGVKWITDKAAVEGDEAKEYWLCWVTTERNEQGPYYAG
                     LTACYLLVNKAIRRGYKSMPEHVNMMDKSMKHHIIIDQIGDENKAILKDFLMNHDEGM
                     WKLSSDALHQAFN"
     gene            complement(720..2015)
                     /locus_tag="NBI57_002169"
     CDS             complement(720..2015)
                     /locus_tag="NBI57_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HCD1823099.1"
                     /translation="MTNAYVDLKLVEEKVFKDPIHRYIHVEDQLIWDLIKTKEFQRLR
                     RIRQLGTLYLSFHTAEHSRFGHSLGVYEIVRRLIDESFIGHDAWDNKDRPLALCAALL
                     HDLGHGPFSHSFEKIFNTDHEAYTQAIITGDTEVNAVLRKVSPEFPREVAEVINKTHH
                     NKLVISMISSQIDADRMDYLQRDAYFTGVSYGAFDMERILRLMRPSKDEVLIKESGMH
                     AVENFIMSRYQMYWQIYFHPVSRGGEVLLNNCLKRAKQLYNEGYEFKLHPHDFIPFFE
                     ETVTIEQYVELDEAVVTYYLEKWTKEDDAILSDLASRFINRDLFKYIPFDGSIITISE
                     LQELFEAGGINPDYYFVSEAFSDLPYDYDRPGSNRKPIHLLRQDGTIREISNQSLVIH
                     SITGINRQDYKLYYPREMVAKIKDKTIREAIENLINELN"
     gene            complement(2055..2531)
                     /locus_tag="NBI57_002170"
     CDS             complement(2055..2531)
                     /locus_tag="NBI57_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCD1823100.1"
                     /translation="MFKVRQATEKDVVQIRDVATKAWFNTYLNIYAATTVNHLLEASY
                     NEHHLKKRLQEQLFLVVEEGNDIVGFANFIYGEELYLSAHYVKPESQHTGYGTALLNE
                     GLSRFEDKFEGVYLEVDNKNEEAVAYYKEQGFTILRSYQPEMYGEKLDLALMYKAF"
     gene            complement(2699..3235)
                     /locus_tag="NBI57_002171"
     CDS             complement(2699..3235)
                     /locus_tag="NBI57_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HCD1823101.1"
                     /translation="MITVLFGGSRPNGNTAQLTKFALQDLEYQWIDVTQHQFKPIRDV
                     RHTAETITSYDDDYLPILDKILASDTIIFASPVYWYSISAPLKAFIEHWSETLQDKRY
                     PNFKAQMAEKDFRVILVGGDCPKIKAKPAITQMKYSLDFLGATLNGYIIGTAEKPGDI
                     MKDNYALARATEWNSILQ"
     gene            complement(3681..4619)
                     /locus_tag="NBI57_002172"
     CDS             complement(3681..4619)
                     /locus_tag="NBI57_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HCD1823102.1"
                     /translation="MKQFVNLGKSDVEVFPIALGTNAVGGHNLYPNLDEEQGKDVVRQ
                     AINHGINLLDTAYIYGPERSEELVGEVVKEYPREQIKIATKGSHEFDENQEVHQNNQP
                     EYLKQQVENSLKRLQTDYIDLYYIHFPDDNTPKDQAVAALQELKEQGKIKAIGVSNFT
                     LDQLKEANKDGYVDVVQLEYNLLHRENEAVLQYCVDHLITFIPYFPLASGILAGKYDE
                     NTKFSDHRTTRRDFIPGVFEENVRRVKALESLAAAHQTSIANIVLAFYLTRPAIDVII
                     PGAKRAEQVIENIKAADIVLSDDEIQYIDELFPIED"
BASE COUNT         1525 a          934 c          673 g         1727 t
ORIGIN      
        1 aatgtgtcag ttattcaaat tttttattaa ctacagatta atttaatttc aaaaaaatta
       61 aaaaaactcc caaccaatct aagactttat tttcacctca agatttacgt cgtcattgag
      121 aaatgaatca tatctttata gattaattgg gaaattttag tttctaatat ttaattaaat
      181 gcttgatgta aagcatcaga agaaagcttc cacatacctt catcatggtt cattaaaaag
      241 tcttttaaaa tagctttatt ctcgtcacca atttgatcaa tgataatatg atgtttcatt
      301 gatttatcca tcatattaac atgttcaggc atacttttat aaccacgacg aattgcttta
      361 ttcactaata aatagcacgc tgttaaacca gcgtaatatg gtccttgttc attacgttct
      421 gttgttaccc aacacaacca atattcttta gcttcatcgc cttcaacagc agctttatca
      481 gtaatccatt tcacaccttt ttcaactttg gcacgagcat gcatgcctcc aatatcaata
      541 aatgcttctt tgttagcaac atcgataaaa actggtgcaa tattgtctaa gctaattgaa
      601 ccaatatttg tacctttatg accatctaga gggtcatttt ttatgatatt aaaattaaag
      661 ccttttttct cagacaatga tataacctcc atatttaacg ataacaattt tagccctgtt
      721 taattaagct cattaatcaa attttcaata gcttctctaa ttgtcttatc tttaatcttt
      781 gcaaccattt ctctaggata atataattta tagtcttggc gattaatgcc tgtaatacta
      841 tgaatgacta atgattgatt gcttatttct ctaatcgtac catcttgtct taataaatga
      901 atcggtttgc gatttgaccc cggtcgatca tagtcatatg gcaaatccga aaatgcttca
      961 ctcacaaaat aataatctgg attaatacca cctgcttcaa acagttcttg cagttctgat
     1021 attgtaataa ttgagccatc aaatggaata tatttaaata agtctcgatt aataaatcga
     1081 cttgctaaat cacttaaaat ggcatcatct tcttttgtcc atttttccaa ataatacgta
     1141 actaccgctt catcgagttc aacatattgt tcaatcgtaa ctgtctcttc aaaaaatgga
     1201 ataaaatcat gtggatgcaa cttaaattca tagccttcat tataaagctg ttttgcgcgt
     1261 ttcaaacaat tattaagcag cacttctcca ccacgactaa ctgggtggaa gtaaatttgc
     1321 caatacattt gataacgact cataataaag ttttcaactg catgcatacc actttctttg
     1381 attagtactt catctttaga aggtcgcatt aatcttaaaa tacgctccat atcaaaagca
     1441 ccatatgata cacctgtgaa atacgcatca cgttgtaaat aatccattct atccgcatcg
     1501 atttgtgacg aaatcatcga aatgaccaat ttattatgat gcgttttatt aattacttcc
     1561 gcaacttctc ttggaaactc aggcgacact ttacgtaata cagcattcac ctcagtatct
     1621 ccagtaataa tcgcttgtgt gtatgcttca tggtctgtat taaatatttt ttcaaaacta
     1681 tgtgaaaatg gaccatgtcc taaatcatgt aataatgcag cacataatgc caacggtcta
     1741 tctttattgt cccatgcatc atgaccaata aatgactcat caattaatcg tctaactatt
     1801 tcatacacac ctaaagaatg tccaaagcga ctatgttctg ctgtgtgaaa agataggtac
     1861 agtgttccta gttgtctaat tcgacgtaac ctttggaatt ccttagtttt aattaaatcc
     1921 catatcaatt gatcttcaac atgaatatat cgatgtatcg gatctttaaa aactttttct
     1981 tctactaatt ttaaatctac atatgcgtta gtcattattc ccctcctttt cgtttaatat
     2041 aatatttaat ttacttaaaa tgctttgtac ataagtgcta agtctaactt ttcgccatac
     2101 atttctggct gataagagcg taagattgta aaaccttgct ctttatagta agctactgct
     2161 tcttcatttt tattatctac ttctaagtaa acaccttcaa atttatcttc aaaacgtgat
     2221 aatccttcat ttaacaatgc tgtaccataa cctgtatgtt gcgattctgg tttaacataa
     2281 tgagctgata aatataattc ttcaccgtaa ataaagttag caaagccaac gatgtcatta
     2341 ccttcttcaa cgactaagaa taattgttct tgaagtcttt tctttaaatg atgttcatta
     2401 tatgaagctt ctaacaagtg attaactgtt gtcgcagcgt atatatttaa gtatgtatta
     2461 aaccaagctt tagttgcgac atctctaatt tgaacaacat ctttttcagt tgcttgtctt
     2521 accttgaaca tgactttctc cccttattaa caagttttaa taacggcatt ataccacaac
     2581 ttgctcaata cttaataaac aatgattgtc tattcaattt atatatttat attttccgtt
     2641 aaaattaaaa ataaaaataa cgaagcaaaa aatcacttcg tttagtatga ggtatgtctt
     2701 attgcaatat actattccac tcagttgcac gtgctaaggc atagttgtct ttcatgatgt
     2761 caccaggctt ttcagcagtt ccaataatat aaccatttaa agtggcacct aaaaagtcta
     2821 aactatattt catttgcgta attgctggct tcgcttttat ttttggacaa tctccaccaa
     2881 ctaaaataac tctaaaatcc ttttcggcca tttgtgcctt aaaattagga tatcgtttat
     2941 cttgtaatgt ttctgaccaa tgttcgataa atgctttcaa tggtgctgaa atgctatacc
     3001 aatacactgg tgatgcaaaa ataattgtat cactagccaa tattttatct agaatcggca
     3061 aatagtcatc gtcatatgaa gtaatagtct ctgctgtatg tctcacgtca cgtatcggtt
     3121 taaactgatg ttgtgtcacg tcaatccatt gatactctaa atcttgcaaa gcgaattttg
     3181 ttaattgtgc agtattaccg tttggtctac tcccaccaaa caaaacagta atcattttag
     3241 cctaacctca cttttgatta ataaatatct gtgtttttcg ttacctaatt atactatcat
     3301 aagctttgcc taccgaatag taaaacgctt acaactttta tataaatttg acgaaatttc
     3361 gtcatgcctt atataacgtc gtttgtgatg ggagtgggac agaaatgata ttttcgaaaa
     3421 atttatttcg tcgtcccacc ccaacttgca cattattgta agctgacttt tcgtcagctt
     3481 ctgtgttggg gcccacaccc caacttgcat tgcctgtaga atttcttttc gaaattctct
     3541 gtgttggggc ccctgactag gattgaaaaa agcttgttac aagcgcattt tcgttcagtc
     3601 aactactgcc aatataactt cgtagagcat agaacattga tttatgtccc agcctgatat
     3661 caccattaaa tacaattcat ttagtcttca attggaaaca attcatcgat atattgaatc
     3721 tcatcatctg ataaaacgat atctgcagct ttaatatttt caatgacttg ttctgcacgt
     3781 tttgcaccag gaataatcac atcgatagct ggtctcgtta aataaaatgc taatacaatg
     3841 ttcgcaattg aagtttgatg tgctgcagct aggctttcca aagcttttac gcgacgcaca
     3901 ttctcttcaa aaacaccagg tataaaatca cgacgtgtag tacgatggtc actaaattta
     3961 gtgttctcat catattttcc agctaaaata ccggatgcta atgggaaata aggaataaat
     4021 gtgattaggt gatcaacaca atattgcaat actgcctcat tttcgcggtg caataaatta
     4081 tattctaact gtacaacatc aacgtaacca tctttatttg cttctttaag ttgatctaat
     4141 gtgaaatttg atacaccaat ggctttaatc ttcccttgtt ccttaagctc ttgtaatgct
     4201 gcaactgctt gatctttcgg agtgttgtca tcaggaaaat gaatataata taaatcgata
     4261 taatcagttt gtagacgttt caaactattc tcaacttgtt gttttaaata ttccggttga
     4321 ttgttctgat gtacttcttg attttcatca aattcatgag accctttcgt agcaatttta
     4381 atttgctctc gcggatattc cttaacaact tctccaacca attcttctga tcgttctggc
     4441 ccataaatat atgccgtatc taataaatta ataccatgat taatggcttg acgaacaaca
     4501 tcttttcctt gttcttcatc taagttcgga tataaattat gcccacctac tgcgttcgtc
     4561 ccaagtgcga ttggaaacac ttcaacatca gatttaccta agtttacaaa ttgcttcatt
     4621 agacccagct ccttatcttt ataacttttc cactatcaca ctatcatgat ttaatacaac
     4681 ttccaaatgt tagaattagg gtttgaatta aggcggtgta ttagaaaaat gttatgtaga
     4741 actcagttaa aataagtatt gcttaagttg atatgcatat agcgcgattg ctcgttgacc
     4801 tttaaattac atgtactttt gaatcagaaa ttgaaaaaca tttaagaacg acaatatta
//
LOCUS       DAHHSC010000175         4854 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.176, whole genome shotgun sequence.
ACCESSION   DAHHSC010000175 DAHHSC010000000
VERSION     DAHHSC010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 4854)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4854)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4854
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.176"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            <1..285
                     /locus_tag="NBI57_002173"
     CDS             <1..285
                     /locus_tag="NBI57_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001801842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Csa1 family protein"
                     /protein_id="HCD1823103.1"
                     /translation="DLKNYQDGSITTNENVPRYEAEYKLNNSDTNVKKLRDIYPITTK
                     KAPILKLHIDGDIKGSSVGYKKIEYKFSKVKDQETTLRDYLNFGPSDEDS"
     gene            333..1117
                     /locus_tag="NBI57_002174"
                     /pseudo
     CDS             333..1117
                     /locus_tag="NBI57_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001836845.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein"
     gene            1162..1923
                     /locus_tag="NBI57_002175"
     CDS             1162..1923
                     /locus_tag="NBI57_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000649608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein"
                     /protein_id="HCD1823104.1"
                     /translation="MCVVYRTSVLILLASGCSGVFDTPEDSKETQIKKSFAKTLDMYP
                     IKNLEDLYDKEGYRDGEFKKGDKGTWVVRSEMIIQPKGKSLTSRGMILYMNRNTRTTT
                     GYFSIEEIDSRKSLDERETEKKYPVKMINNKIIPTEEIKDEKLKKEIENFKFFVQYGS
                     FKGIENYENGDISYNSEAPIYSAKYKLKNDDYNVKELRKRYNIPTEKAPKLLLKGSGD
                     LKGSSVGYKEIEFIFIENKKENIYFSDGLNLIPSD"
     gene            1984..2769
                     /locus_tag="NBI57_002176"
     CDS             1984..2769
                     /locus_tag="NBI57_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001269759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein"
                     /protein_id="HCD1823105.1"
                     /translation="MRDSKRVVLYISIMVLSIFIIGCGKGNEIKEDAKEEQIKKSFAK
                     TLDMYPIKNLEDLYDKEGYRDGEFKKGDKGMWTIYTDFAKSNRPGVLDNEGMVLNLDR
                     NTRTAKGYYFVDTIYDNHENSYSKNYRVEMKNNKIILLDKVEDQKLKERIENFKFFGQ
                     YADFKSLKSYNHGDVSINSNVPSYDAKFKMSNKDENVKQLRSRYNIPTDKAPILKMHI
                     DGDLKGSSVGYKKLEIDFSKEENSELSIVDSLNFQPAKNKDDE"
     gene            2788..3570
                     /locus_tag="NBI57_002177"
     CDS             2788..3570
                     /locus_tag="NBI57_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001557596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein"
                     /protein_id="HCD1823106.1"
                     /translation="MGYSKRFALYISILILIVMVAGCGKSDETKEDSKEEQIKKSFAK
                     TLDMYPIKNLEDLYDKEGYRDGEFKKGDKGTWVLYSAIVSQPKGESLKSRGMILKLDR
                     NKRTAKGSYIIRELKEDKNHDVQKNEKKYPVKLVNNRIVLVKDVKDKKLKNEIESFEL
                     FSQYGNFNHFDRNEITNISYNPNAPNYSAEYKMKKNDRNIQQLKKRFNLKTSKTPKLL
                     FKGSGDIKGSSVGYKEIEIIFSRSKEEAFIMLTALSSFQVTK"
     gene            3630..4421
                     /locus_tag="NBI57_002178"
     CDS             3630..4421
                     /locus_tag="NBI57_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001802015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein"
                     /protein_id="HCD1823107.1"
                     /translation="MGYLKRLVLYIVIMVMSVFIIGCDKSSDTAENPKEGSKEAQIKK
                     SFSKTLDMYPIKNLENLYDKEGYRDGEFKKGDKGTWTISTDFAKSNKQGEMNSEGMVL
                     HFNRNTRTATGYYTVRTTYDEVDKLAREKKYRVEFKNNKIVLLDKVEDENLKQKIENF
                     KFFGQYADFKDLKNYKNGRISSNENVPYYEAEYKRNNSDGNVKKLREKYPITTKQSPI
                     LKLHIDGDIKGSSVGYKQIEYTFSKEKDDETFMSDFLNFGPSHSK"
     gene            4473..>4854
                     /locus_tag="NBI57_002179"
     CDS             4473..>4854
                     /locus_tag="NBI57_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001797296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein"
                     /protein_id="HCD1823108.1"
                     /translation="MGYFKRVVLYIIVMVVSVFIIGCNKSSDTSEKPKEDSKETQIKK
                     SFAKTLDMYPIKNLEDLYDKEGYRDGEFKKGDKGMWTIYTDFAKSNKQGGLSNEGMVL
                     YLDRNTRTAKGHYFVKTFYNKGKFP"
BASE COUNT         2073 a          505 c          953 g         1323 t
ORIGIN      
        1 gatttaaaaa attatcaaga tggaagtata acaactaatg aaaatgtacc gagatatgaa
       61 gcagaataca aattgaataa tagtgataca aatgtaaaaa aacttagaga tatttatcca
      121 attacaacga aaaaggctcc aatattaaag ttacatatag atggtgatat aaaaggaagt
      181 tcagttggat acaaaaaaat agaatataaa ttttcaaaag ttaaagatca agagacaaca
      241 ttaagagatt atttaaattt tgggccgtct gatgaagata gctaaagttt attagatgta
      301 gaaataattc taaagcagag agacgtgaaa tgatgggata ttcaaaaagg tttgcattgt
      361 acataagcgt tatgatttta atatttgcga tagcaggttg tggcaaaagt gatgaaacaa
      421 aagaaggttc caaagaagaa caaattaaaa agagctttgc gaaaacgtta gatatgtacc
      481 caattaagaa tctcgaggac ttatatgaca aagaaggata tcgagatggc gaatttaaaa
      541 agggtgacaa agggacatgg gttatcagtt cagtaatggt taaacagcca aaaggtgaaa
      601 ttatgaaatc aagagggatg tatctatttt taaatagaaa tacaagaacc gcaaaaggat
      661 attttattgt agatgaaaca agtaatgata ctttaaaaaa acagaggata aagaaaaacg
      721 ttatccagta aagatggtga ataataaaat agtacctatt gatccaatta atgataaagg
      781 tgtaaaaaaa gaaatagaaa attttaagtt tttctcacaa tatggtgatt ttaaagaatt
      841 aaaaaattat aaaaatggag aggtttcata caattctgaa gcccctatct actcagcaaa
      901 atatcaactg aaaaataatg attataatgt aaagcaatta cgtaaaagat atgatatatc
      961 aacagaaaag gctcccaaat tgttgttgaa aggtactgga gatttaaaag gttcatcaat
     1021 cggacataag gacattgagt ttacattcgt ggagaatcaa gaagagaata tattttttac
     1081 tgatagtttg gagtttacct caagtgagaa ctattagcta tgataaaaaa ggggatacgg
     1141 aacaatgaat aattttagaa aatgtgcgtt gtgtatagga cgtcagtctt aattctatta
     1201 gcatcaggtt gcagtggcgt atttgacact cctgaagatt caaaagaaac acaaatcaaa
     1261 aagagctttg cgaaaacgtt agatatgtat ccaattaaaa atcttgagga tttatacgat
     1321 aaagaaggat atcgagatgg agaatttaaa aagggagaca aagggacgtg ggttgttaga
     1381 tctgaaatga taattcaacc aaaaggtaaa agtttaactt caagaggcat gatattatac
     1441 atgaatagaa atactaggac cacaaccggt tatttttcaa tagaagaaat agattcaaga
     1501 aaaagccttg atgaaagaga aacagaaaaa aagtatcctg tgaaaatgat aaacaataaa
     1561 attattccaa ctgaggagat aaaagatgaa aagttgaaaa aggaaattga aaactttaag
     1621 ttttttgtgc aatatggcag ttttaaagga atagagaatt atgaaaatgg tgacatttcc
     1681 tataattctg aagctcctat ttattcagcg aaatataaac tgaaaaatga tgattataat
     1741 gttaaagaat tacgaaaaag atataatatt ccaacagaaa aggcgcctaa attgttgttg
     1801 aaaggttcgg gggatttgaa agggtcttca gttggatata aggaaattga atttatattt
     1861 atagaaaata aaaaagaaaa tatatatttt tcagatggat taaacttaat tccaagtgac
     1921 taaaaataac acaaaggttg atagtactca acaaatatta acatgataga gagaagtgaa
     1981 aaaatgaggg attcaaaaag agtagtattg tacataagca ttatggtgtt gagtattttt
     2041 ataattggtt gtggcaaagg taatgaaata aaagaagacg caaaggaaga acaaatcaaa
     2101 aagagctttg cgaaaacgtt agatatgtat ccaatcaaaa atctcgagga cctatatgat
     2161 aaagaaggat atcgagatgg cgaatttaaa aaaggtgaca aagggatgtg gacgatttac
     2221 acagatttcg ccaaaagtaa tagaccaggt gtattagata atgaaggtat ggttttaaat
     2281 ttggatagaa atacacgaac ggccaaggga tattattttg tagatactat atatgacaat
     2341 catgaaaact cttatagtaa aaattataga gttgagatga aaaacaataa aattatttta
     2401 ttagacaagg tggaagatca aaaacttaaa gaaagaatag aaaactttaa atttttcgga
     2461 caatatgccg atttcaagag tttgaaaagt tacaaccatg gcgacgtttc aattaatagt
     2521 aatgttccaa gttatgacgc gaaatttaaa atgagtaata aagatgaaaa tgttaagcaa
     2581 ttaagaagcc gttataacat tcctactgat aaagctccaa tattaaaaat gcatattgat
     2641 ggggacttaa aaggcagttc cgttggatat aaaaagttag aaatagactt ttcaaaagaa
     2701 gaaaatagcg aattatcaat agtcgattca ttaaattttc aacctgccaa aaataaagat
     2761 gatgaatgat agggaggatg tgaagcgatg ggatattcaa aaaggtttgc attgtacata
     2821 agtatcttga ttttaatagt tatggtagca ggttgtggta aaagtgatga aacaaaagaa
     2881 gattccaaag aagaacaaat taaaaagagc tttgcgaaaa cgttagatat gtaccctatt
     2941 aagaatctcg aggatttata cgataaagaa ggataccgtg atggcgaatt taaaaagggt
     3001 gataagggga cgtgggttct gtattcagct atagtatcac aaccaaaagg cgagagttta
     3061 aaatcacgag gaatgatatt aaagttagat agaaataaga gaactgctaa aggaagttat
     3121 attattagag aattgaaaga agataaaaat catgatgttc aaaaaaatga aaagaaatat
     3181 ccagtgaaat tggtgaataa taggatagtt ttggtaaaag atgttaaaga caaaaagtta
     3241 aaaaatgaaa tagagtcgtt tgaattattt tcacaatatg gaaactttaa tcattttgat
     3301 cggaatgaga ttactaatat ttcatataat cctaatgctc ccaattactc tgcagaatat
     3361 aaaatgaaga aaaatgacag aaacattcaa cagttgaaaa agagatttaa tctaaaaact
     3421 agcaagacac caaaattatt gtttaaggga tctggagata taaaggggtc ttctgtagga
     3481 tataaggaaa tagaaatcat atttagtaga agtaaagaag aagcatttat tatgttgaca
     3541 gcgttgagtt cgttccaagt aacaaaatga caaaaagttg atattagtca acaaaagcaa
     3601 atattgagaa taaagagagt tgtgaaatga tgggttattt aaaaagactt gtattgtaca
     3661 tagttattat ggttatgagt gtttttataa taggttgtga taaatcaagc gatactgcag
     3721 aaaatccaaa agaaggttca aaagaagcac aaattaaaaa gagtttttcg aaaacgttag
     3781 atatgtatcc aattaagaat ctcgagaatc tatatgacaa agaaggatat cgtgatggcg
     3841 aatttaaaaa aggtgataaa gggacttgga caatatcaac agattttgct aaaagcaaca
     3901 agcaaggtga aatgaatagc gaaggtatgg tactacattt taatagaaat acgaggacag
     3961 caacaggata ttatactgta agaacaactt atgatgaagt ggataagttg gcacgcgaaa
     4021 aaaaatatcg tgttgaattt aaaaacaata agatagttct attggacaaa gtagaagatg
     4081 aaaatcttaa acaaaaaata gaaaatttta aatttttcgg gcaatatgcc gattttaaag
     4141 acttgaaaaa ttataaaaat ggaagaatat ctagcaatga aaatgttcct tattatgaag
     4201 cagagtacaa aaggaataat agtgatggaa atgtaaaaaa acttagagaa aagtacccaa
     4261 ttacaaccaa gcagtctcca atattaaaac tgcatataga cggcgatatt aaaggtagct
     4321 cagttggata taaacagata gaatacacat tttctaagga gaaagatgat gagactttta
     4381 tgagtgattt tttgaacttc ggtccatcac acagtaaata gtaaataaaa ccaagggcat
     4441 aaactaattt aaaacaagag agaagtgaaa agatgggata ttttaaaaga gttgtacttt
     4501 acataattgt tatggttgtg agtgttttta taataggttg caataaatca agcgatactt
     4561 cagaaaagcc aaaagaagat tcaaaagaaa cacaaatcaa aaagagcttt gcgaaaacgt
     4621 tagatatgta tccaattaag aatctcgagg acttatatga taaagaagga taccgtgatg
     4681 gcgaatttaa aaagggtgac aaaggaatgt ggactatata tacagatttt gctaaaagta
     4741 ataaacaagg tggattgagt aatgaaggta tggtcttata cttagataga aatacacgga
     4801 ctgcaaaggg acattatttt gttaagacat tctataataa gggcaaattc ccag
//
LOCUS       DAHHSC010000176         4789 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.177, whole genome shotgun sequence.
ACCESSION   DAHHSC010000176 DAHHSC010000000
VERSION     DAHHSC010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 4789)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4789)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4789
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.177"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            181..336
                     /locus_tag="NBI57_002180"
     CDS             181..336
                     /locus_tag="NBI57_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1823109.1"
                     /translation="MMVKKLLKAIKQNFLYEQSNITFKMIVFGYKRLSKMLFQFEINH
                     INFLWEG"
     gene            344..865
                     /locus_tag="NBI57_002181"
     CDS             344..865
                     /locus_tag="NBI57_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1823110.1"
                     /translation="MINIISAIGSIGTFIMALFYFVSVSVQLYQMKISFLPALGFNQI
                     LLEREKDQLNIMNSATEEHHHKDYIKLYNLGGGAAKKIAIEVLLGNDKVIQKKYVNIL
                     PSKEGYMLPINKNVYEELERTIENNGYEADLNVRMTYYHNVSRKQQEVILKGQIDRFN
                     TYNNKEIYDLQFI"
     gene            1004..1534
                     /locus_tag="NBI57_002182"
     CDS             1004..1534
                     /locus_tag="NBI57_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCD1823111.1"
                     /translation="MQIRQIHQHDFAQVDQLIRTAFENSEHGYGNESELVDQIRLSDT
                     YDNNLEIVAVLQNEVVGHGLLSEVYLDNGAQREIGLVLAPVSVDIHHQNKGIGKRLIQ
                     ALEREAILKGYNFISVLGWPTYYANLGYQRASMYDIYPPYDGIPDEAFLIKELKVNSL
                     AGKTGTINYTSAFEKI"
     gene            1797..4766
                     /gene="clfA"
                     /locus_tag="NBI57_002183"
     CDS             1797..4766
                     /gene="clfA"
                     /locus_tag="NBI57_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MSCRAMM family adhesin clumping factor ClfA"
                     /protein_id="HCD1823112.1"
                     /translation="MNMKKKEKHAIRKKSIGVASVLVGTLIGFGVLSSKEADASENSV
                     TQSDSASNESKSNDSSSVSAAPKTDDTNVSDTKTSSNTNNGETSVAQNPAQQETTQSS
                     STNATTEETPVTGEATTTTTNQANTPATTQSSNTNAEELVNQTSNETTSNDTNTVSSV
                     NSPQNSTNAENVSTTQDTSTEATPSNNESAPQNTDASNKDVVSQAVNPSTPRMRAFSL
                     AAVAADAPAAGTDITNQLTDVKVTIDSGTTVYPHQAGYVKLNYGFSVPNSAVKGDTFK
                     ITVPKELNLNGVTSTAKVPPIMAGDQVLANGVIDSDGNVIYTFTDYVDNKENVTANIT
                     MPAYIDPENVTKTGNVTLTTGIGTNTASKTVLIDYEKYGQFHNLSIKGTIDQIDKTNN
                     TYRQTIYVNPSGDNVVLPALTGNLIPNTKSNALIDAKNTDIKVYRVDNANDLSESYYV
                     NPSDFEDVTNQVRISFPNANQYKVEFPTDDDQITTPYIVVVNGHIDPASTGDLALRST
                     FYGYDSNFIWRSMSWDNEVAFNNGSGSGDGIDKPVVPEQPDEPGEIEPIPEDSDSDPG
                     SDSGSDSNSDSGSDSGSDSTSDSGSDSASDSDSASDSDSASDSDSASDSDSASDSDSA
                     SDSDSASDSDSASDSDSASDSDSASDSDSASDSDSASDSDSASDSDSDSDSDSDSDSD
                     SDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSD
                     SDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSD
                     SDSDSDSDSDSDSDSDSDSDSDSASDSDSDSDSESDSDSDSDSDSDSDSDSDSDSDSE
                     SDSDSDSDSDSESDSDSDSDSDSDSASDSDSGSDSDSSSDSDSDSTSDTGSDNDSDSD
                     SNSDSESGSNNNVVPPNSPKNGTNASNKNEAKDSKEPLPDTGSEDEANTSLIWGLLAS
                     LGSLLLFRRKKENKDKK"
BASE COUNT         1734 a          754 c          931 g         1370 t
ORIGIN      
        1 ttgatttgtt aagaaattac gtttgtaaaa ataaaaaaat caacttattt gtatgagatg
       61 aatatgtatt gaagaagatg tgttattaat ttggaaaata ctggtcaaag atgggagctc
      121 ttaaaagcgt tattgtattt tttagtcaat gcaaatagat tgccgtaata ataatcgtac
      181 ttgatggtta aaaaattact taaggctata aagcaaaact ttttatatga gcagtcgaat
      241 ataacgttta aaatgattgt ttttggatat aaacgattaa gtaaaatgct ttttcagttt
      301 gaaattaatc atataaattt cttatgggaa ggttgatatc ttaatgatta atatcatttc
      361 agctatagga tctattggaa catttattat ggctttattt tattttgtat cagtttcagt
      421 tcaactttat caaatgaaaa ttagctttct gccagcttta ggttttaacc aaattttatt
      481 agaaagggag aaggatcaac ttaatataat gaattcggca acagaagagc atcatcataa
      541 agattatatt aaactatata atttaggtgg cggtgctgct aaaaaaattg caatagaggt
      601 tttattgggt aatgataaag tcattcagaa aaaatacgtg aatattttac ctagtaaaga
      661 agggtacatg ttaccaatta ataaaaatgt gtacgaagaa ttagaaagaa cgattgagaa
      721 taatggttat gaagctgatt tgaatgtacg tatgacttat tatcataatg taagtcgcaa
      781 acaacaggaa gttatattaa aaggtcaaat cgaccgtttt aatacttata ataataaaga
      841 aatttatgat ttgcagttta tctaaaaatt gatttaagag ggtagttgtt tattgcgaaa
      901 aatatcattc aattttaatg aaataatggc gtcattacta taaaatatta ctttatgttg
      961 taatgcattt ttctataaga tagaactaaa aggaggggca aagatgcaaa ttagacaaat
     1021 acatcaacat gactttgctc aagttgacca gttaattaga acggcatttg aaaatagtga
     1081 acatggttat ggtaatgaat cagagctagt agaccaaatt cgtctaagtg atacgtatga
     1141 caataactta gaaatagtag ctgttcttca aaacgaagtt gtagggcacg gtttactaag
     1201 tgaagtttat cttgataacg gagcacaacg ggaaattgga ttagtgttag cacctgtatc
     1261 tgttgatatt catcatcaaa ataaaggtat tgggaagcga ttgattcaag cattagaacg
     1321 agaagcaata ttaaaaggat ataattttat cagtgtatta ggatggccga cgtattatgc
     1381 caatctagga tatcaacgcg caagtatgta cgacatttat ccaccatatg atggtatacc
     1441 agacgaagcg tttttaatta aagaattaaa agtgaacagt ttagcgggaa aaacaggtac
     1501 cataaattac acatctgctt ttgaaaaaat atgatttaaa gctaggatta cattaggtag
     1561 agttcatatt aataataaaa aatgtttgca atcaaatcgt acgttgtcgt ttgtaattct
     1621 taaaatagca ataaataaaa tgtttgttag taaagtatta ttgtggataa taaaatatcg
     1681 atacaaatta attgctataa tgcaatttta gtgtataatt ccattaacag agattaaata
     1741 tatctttaaa gggtatatag ttaatataaa atgacttttt aaaaagaggg aataaaatga
     1801 atatgaagaa aaaagaaaaa cacgcaattc ggaaaaaatc gattggcgtg gcttcagtgc
     1861 ttgtaggtac gttaatcggt tttggagtac tcagcagtaa agaagcagat gcaagtgaaa
     1921 atagtgttac gcaatctgat agcgcaagta acgaaagcaa aagtaatgat tcaagtagcg
     1981 ttagtgctgc acctaaaaca gacgacacaa acgtgagtga tactaaaaca tcgtcaaaca
     2041 ctaataatgg cgaaacgagt gtggcgcaaa atccagcaca acaggaaacg acacaatcat
     2101 catcaacaaa tgcaactacg gaagaaacgc cggtaactgg tgaagctact actacgacaa
     2161 cgaatcaagc taatacaccg gcaacaactc aatcaagcaa tacaaatgcg gaggaattag
     2221 tgaatcaaac aagtaatgaa acgacttcta atgatactaa tacagtatca tctgtaaatt
     2281 cacctcaaaa ttctacaaat gcggaaaatg tttcaacaac gcaagatact tcaactgaag
     2341 caacaccttc aaacaatgaa tcagctccac agaatacaga tgcaagtaat aaagatgtag
     2401 ttagtcaagc ggttaatcca agtacgccta gaatgagagc atttagttta gcggcagtag
     2461 ctgcagatgc accggcagct ggcacagata ttacgaatca gttgacagat gtgaaagtta
     2521 ctattgactc tggtacgact gtgtatccgc accaagcagg ttatgtcaaa ctgaattatg
     2581 gtttttcagt gcctaattct gctgttaaag gtgacacatt caaaataact gtacctaaag
     2641 aattaaactt aaatggtgta acttcaactg ctaaagtgcc accaattatg gctggagatc
     2701 aagtattggc aaatggtgta atcgatagtg atggtaatgt tatttataca tttacagact
     2761 atgttgataa taaagaaaat gtaacagcta atattactat gccagcttat attgaccctg
     2821 aaaatgttac aaagacaggt aatgtgacat tgacaactgg cataggaacc aatactgcta
     2881 gtaagacagt attaatcgac tatgagaaat atggacaatt ccataattta tcaattaaag
     2941 gtacgattga tcaaatcgat aaaacaaata atacgtatcg ccaaacaatt tatgtcaatc
     3001 caagcggaga taacgttgtg ttacctgcct taacaggtaa tttaattcct aatacaaaga
     3061 gtaatgcgtt aatagatgca aaaaacactg atattaaagt ttatagagtc gataatgcta
     3121 atgatttatc tgaaagttat tatgtgaatc ctagcgattt tgaagatgta actaatcaag
     3181 ttagaatttc atttccaaat gctaatcaat acaaagtaga atttcctacg gacgatgacc
     3241 aaattacaac accgtatatt gtagttgtta atggccatat tgatcctgct agtacaggtg
     3301 atttagcact acgttcgaca ttttatggtt atgattctaa ttttatatgg agatctatgt
     3361 catgggacaa cgaagtagca tttaataacg gatcaggttc tggtgacggt atcgataaac
     3421 cagttgttcc tgaacaacct gatgagcctg gtgaaattga accaattcca gaggattcag
     3481 attctgaccc aggttcagat tctggcagcg attctaattc agatagcggt tcagattctg
     3541 gcagtgattc tacatcagat agtggttcag attcagcgag tgattcagat tcagcaagtg
     3601 attcagactc agcgagtgat tcagattcag caagtgattc agattcagca agtgattcag
     3661 attcagcaag tgattcagac tcagcaagtg attcagattc agcaagtgat tcagattcag
     3721 caagcgattc agattcagcg agcgattcag attcagcgag cgattcagat tcagcgagtg
     3781 attccgactc agcgagcgat tcagactcag atagtgactc agattccgat agcgattccg
     3841 actcagatag cgactcagat tcagacagcg attctgactc agacagcgat tctgactcag
     3901 acagtgactc agattccgat agcgattccg actcagacag tgactcagat tccgatagcg
     3961 attccgactc agacagtgac tcagattccg atagcgattc agattccgac agtgattccg
     4021 actcagatag cgattccgac tcagatagcg actcagattc agacagcgat tcagattcag
     4081 acagcgattc tgactcagac agtgactcag attccgatag cgattcagat tcagacagtg
     4141 attcagactc agatagcgat tcagattccg acagtgactc agactcagac agcgattcag
     4201 attccgatag cgattcagat tccgacagtg actcagattc cgatagtgac tcggattcag
     4261 cgagtgattc agattcagat agcgattcag aatcagatag tgactcagac tcagacagtg
     4321 attcagattc agatagtgac tcagactcag acagcgattc agaatcagat agtgactccg
     4381 attcagacag cgattcagaa tcagatagtg actccgattc agatagcgat tcggattcag
     4441 cgagtgattc agactcaggt agtgactccg attcatcaag tgattcagat tccgattcaa
     4501 cgagtgacac aggatcagac aacgactcag acagtgattc aaatagcgat tccgagtcag
     4561 gttctaacaa taatgtagtt ccgcctaatt cacctaaaaa tggtactaat gcttctaata
     4621 aaaatgaggc taaagatagt aaagaaccat taccagatac aggttctgaa gatgaagcga
     4681 atacgtcact aatttgggga ttattagcat cattaggttc attactactt ttcagaagaa
     4741 aaaaagaaaa taaagataag aaataagtaa taataatatt aaattaatc
//
LOCUS       DAHHSC010000177         4777 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.178, whole genome shotgun sequence.
ACCESSION   DAHHSC010000177 DAHHSC010000000
VERSION     DAHHSC010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 4777)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4777)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4777
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.178"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            30..902
                     /gene="dprA"
                     /locus_tag="NBI57_002184"
     CDS             30..902
                     /gene="dprA"
                     /locus_tag="NBI57_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000593194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HCD1823113.1"
                     /translation="MIKLFLLKLYWAHFSTKQIHQFLMAYPNVIKEEGRKKDSYLCEW
                     VNREENVLLLRKYYAFIKLDHNDIIKELQKLKVSYITYMDSEYPVLLKEIYQFPLLLF
                     YKGNIKLINNMHHLAVVGARDSTSYTQQSLEFLLSNDKSKYLTIVSGLAQGADAMAHQ
                     IALKYNLPTIAVLAFGHQTHYPKSTLALRNKIEEKGLVISEYPPHTPIAKYRFPERNR
                     IISGLSKGVLITEAKEQSGSHITIDFALEQNRNVYVLPGSMFNPMTKGNLLRIQEGAK
                     VVLNANDIFEDYYI"
     gene            1076..3151
                     /gene="topA"
                     /locus_tag="NBI57_002185"
     CDS             1076..3151
                     /gene="topA"
                     /locus_tag="NBI57_002185"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HCD1823114.1"
                     /translation="MTLADNLVIVESPAKAKTIEKYLGKKYKVIASMGHVRDLPRSQM
                     GVDTEDNYEPKYITIRGKGPVVKELKKHAKKAKNVFLASDPDREGEAIAWHLSKILEL
                     EDSKENRVVFNEITKDAVKESFKNPREIEMNLVDAQQARRILDRLVGYNISPVLWKKV
                     KKGLSAGRVQSVALRLVIDRENEIRNFKPEEYWTIEGEFRYKKSKFNAKFLHYKNKPF
                     KLKTKKDVEKITAALDGDQFEITNVTKKEKTRNPANPFTTSTLQQEAARKLNFKARKT
                     MMVAQQLYEGIDLKKQGTIGLITYMRTDSTRISDTAKAEAKQYITDKYGESYTSKRKA
                     SGKQGDQDAHEAIRPSSTMRTPDDMKSFLTKDQYRLYKLIWERFVASQMAPAILDTVS
                     LDITQGDIKFRANGQTIKFKGFMTLYVETKDDSDSEKENKLPKLEQGDKVTATQIEPA
                     QHYTQPPPRYTEARLVKTLEELKIGRPSTYAPTIDTIQKRNYVKLESKRFVPTELGEI
                     VHEQVKEYFPEIIDVEFTVNMETLLDKIAEGDITWRKVIDGFFSSFKQDVERAEEEME
                     KIEIKDEPAGEDCEVCGSPMVIKMGRYGKFMACSNFPDCRNTKAIVKSIGVKCPKCND
                     GDVVERKSKKNRVFYGCSKYPECDFISWDKPIGRDCPKCNQYLVENKKGKTTQVICSN
                     CDYKEAAQK"
     gene            3307..4614
                     /gene="trmFO"
                     /locus_tag="NBI57_002186"
     CDS             3307..4614
                     /gene="trmFO"
                     /locus_tag="NBI57_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate--tRNA-(uracil(54)-
                     C(5))-methyltransferase (FADH(2)-oxidizing) TrmFO"
                     /protein_id="HCD1823115.1"
                     /translation="MTQTVNVIGAGLAGSEAAYQLAERGIKVNLIEMRPVKQTPAHHT
                     DKFAELVCSNSLRGNALTNGVGVLKEEMRRLNSIIIEAADKARVPAGGALAVDRHDFS
                     GYITETLKNHENITVINEEINAIPDGYTIIATGPLTTETLAQEIVDITGKDQLYFYDA
                     AAPIIEKESIDMDKVYLKSRYDKGEAAYLNCPMTEDEFNRFYDAVLEAEVAPVNSFEK
                     EKYFEGCMPFEVMAERGRKILLFGPMKPVGLEDPKTGKRPYAVVQLRQDDAAGTLYNI
                     VGFQTHLKWGAQKEVIKLIPGLENVDIVRYGVMHRNTFINSPDVLNEKYELISQPNIQ
                     FAGQMTGVEGYVESAASGLVAGINLAHKILGKGEVVFPRETMIGSMAYYISHAKNNKN
                     FQPMNANFGLLPSLETRIKDKKERYEAQANRALDYLENFKKTL"
BASE COUNT         1828 a          696 c          885 g         1368 t
ORIGIN      
        1 ttgtatgatt acaaaaagga gttgatttat tgattaaact atttttgctt aagttatact
       61 gggcacactt ttcgactaaa caaattcatc aatttttaat ggcatatcct aatgtaatta
      121 aagaggaggg aagaaaaaaa gatagttatt tatgtgaatg ggtgaatagg gaagaaaatg
      181 ttcttttatt acgtaaatac tatgctttta taaaacttga tcataacgat attattaaag
      241 aactgcagaa attaaaagta agttacatta catatatgga ttctgaatac ccagtgctat
      301 taaaagaaat atatcaattt ccattacttc ttttctataa agggaacatc aaattaataa
      361 ataatatgca tcatttggca gtagtaggtg caagagattc tacaagttat acccaacagt
      421 ctttagaatt tttattatca aatgataaaa gcaaatattt aacaattgtt tccggccttg
      481 ctcaaggagc tgatgcaatg gcacatcaaa tagctttaaa atacaatctc cctacaattg
      541 cagttttagc ctttggccat caaacacatt atcccaaaag tacattagca ttaagaaata
      601 aaatagaaga aaaaggttta gttatatccg aatatccacc acatacacca attgctaaat
      661 atagatttcc tgagcgcaat agaattatca gcggtttgtc aaaaggggtt ttaattactg
      721 aggctaagga acaaagtggc agtcacatca cgatagattt tgcattagag caaaatagaa
      781 atgtttatgt tttacctgga tctatgttta atcctatgac aaaaggtaat ttattacgta
      841 tccaagaagg tgctaaggta gtattaaacg ctaatgatat atttgaagac tactatattt
      901 aaaactaaaa ataacgagtt gcttcatatt tcttttgtta ccctttgtaa tttatttttt
      961 aataaaatct ataaaaaata gacagggaaa atgatttgtt tagatataaa acgttgacaa
     1021 aagcaaaatt aagcgtttat catttatctt tagtaattag attagcgagg gggaaatgac
     1081 attggcagat aatttagtca ttgttgaatc gcctgcaaaa gcaaaaacca ttgaaaagta
     1141 tttaggtaag aaatataaag ttatagcttc aatgggacac gtcagagact taccaagaag
     1201 tcaaatgggt gtcgacactg aagataatta cgaaccaaaa tatataacaa tacgcggaaa
     1261 aggtcctgtt gtaaaagaat tgaaaaaaca tgcaaaaaaa gcgaaaaacg tctttctcgc
     1321 aagtgacccc gaccgtgaag gtgaagcaat tgcttggcat ttatcaaaaa ttttagagct
     1381 tgaagattct aaagaaaatc gcgttgtttt caacgaaata actaaagacg ctgttaaaga
     1441 aagttttaaa aatcctagag aaattgaaat gaacttagtc gatgcacaac aagcgcgtcg
     1501 aatattagat agattggttg gctataacat ctcgccagtt ctatggaaaa aagtgaaaaa
     1561 aggtttgtca gcgggtcgag ttcaatctgt tgcacttcgt ttagtcattg accgtgaaaa
     1621 tgaaattcga aactttaaac cagaagaata ttggactatt gaaggagaat ttagatacaa
     1681 aaaatcaaaa ttcaatgcta aattccttca ttataaaaat aaacctttta aattaaaaac
     1741 gaaaaaagat gttgagaaaa ttacagctgc actagatgga gatcaattcg aaattacaaa
     1801 cgtgactaaa aaagaaaaaa cgcgtaatcc agcaaaccca tttacaactt ctacattaca
     1861 acaagaggcg gcacgtaaat taaactttaa agcaagaaaa acaatgatgg tcgcacaaca
     1921 attatatgaa ggtatagatt tgaaaaaaca aggtacgatt ggtttaataa catatatgag
     1981 aaccgattct acacgtattt cagatactgc caaagctgaa gcaaaacagt atataactga
     2041 taaatacggt gaatcttaca cttctaaacg taaagcatca gggaaacaag gtgaccaaga
     2101 tgcccatgag gctattagac cttcaagtac tatgcgtacg ccagatgata tgaagtcatt
     2161 tttgacgaaa gaccaatacc gattatacaa attaatttgg gaacgatttg ttgctagtca
     2221 aatggctcca gcaatacttg atacagtctc attagacata acacaaggtg acattaaatt
     2281 tagagcgaat ggtcaaacaa tcaagtttaa aggatttatg acactttatg tagaaactaa
     2341 agatgatagt gatagcgaaa aggaaaataa actgcctaaa ttagagcaag gtgataaagt
     2401 cacagcaact caaattgaac cagctcaaca ctatacacaa ccacctccaa gatatactga
     2461 ggcgagatta gtaaaaacac tagaagaatt gaaaattggg cgaccatcaa cttatgcacc
     2521 gacaatagat acgattcaaa agcgtaacta tgtcaaatta gaaagtaagc gttttgttcc
     2581 tactgagttg ggagaaatag ttcatgaaca agtgaaagaa tacttcccag agattattga
     2641 tgtggaattc acagtgaata tggaaacgtt acttgataag attgcagaag gcgacattac
     2701 atggagaaaa gtaatagacg gtttctttag tagctttaaa caagatgttg aacgtgctga
     2761 agaagagatg gaaaagattg aaatcaaaga tgagccagcc ggtgaagact gtgaagtttg
     2821 tggttctcct atggttataa aaatgggacg ctatggtaag ttcatggctt gctcaaactt
     2881 cccggattgt cgtaatacaa aagcgatagt taagtctatt ggtgttaaat gtccaaaatg
     2941 taatgatggt gacgtcgtag aaagaaaatc taaaaagaat cgtgtctttt atggatgttc
     3001 gaaatatcct gaatgcgact ttatctcttg ggataagccg attggaagag attgtccaaa
     3061 atgtaaccaa tatcttgttg aaaataaaaa aggcaagaca acacaagtaa tatgttcaaa
     3121 ttgcgattat aaagaggcag cgcagaaata atatttttat tccctagaga cattttaaga
     3181 ttgttaaata gaatcattag tgaatcttat tttaaagata gtaatggatt aatctaaata
     3241 agtgcggata atataaacat aacaacataa ttaaaagaca taaatgacaa taaaaggagt
     3301 atagaaatga ctcaaactgt aaatgtaata ggtgctggtc ttgccggttc agaagcggca
     3361 tatcaattag ctgaaagagg aattaaagtt aatctaatag agatgagacc tgttaaacaa
     3421 acaccagcgc accatactga taaatttgcg gaacttgtat gttccaattc attacgcgga
     3481 aatgctttaa ctaatggtgt gggtgtttta aaagaagaaa tgagaagatt gaattctata
     3541 attattgaag cggctgataa ggcacgagtt ccagctggtg gtgcattagc agttgataga
     3601 cacgattttt caggttatat tactgaaaca cttaaaaatc atgaaaatat cacagttatt
     3661 aatgaagaaa ttaatgccat tccagatgga tacacaatta tcgcaacagg accacttact
     3721 acagaaaccc ttgcgcaaga aatagtggac attactggta aagatcaact ttatttctat
     3781 gatgcagctg ctccaattat tgaaaaagaa tctattgata tggataaagt ttacttaaag
     3841 tcccgttatg ataaaggtga agctgcatat ttaaactgtc ctatgactga ggatgaattt
     3901 aatcgctttt atgatgcagt attagaagct gaagttgcac ctgtaaattc atttgaaaaa
     3961 gaaaaatatt tcgagggttg tatgcctttt gaagtaatgg cagaacgcgg gcgcaagata
     4021 ttactatttg gaccaatgaa accagtagga ttagaagatc caaagactgg gaaacgtcct
     4081 tatgcagtgg ttcaattaag acaagatgac gctgctggca cactctacaa tattgtcggc
     4141 ttccaaacac atttaaaatg gggagctcaa aaagaagtca ttaaattaat tccaggttta
     4201 gagaatgttg atattgttag atatggtgtg atgcatagaa ataccttcat taactcaccg
     4261 gacgtattaa acgaaaaata tgaattgatt tcacaaccaa acatacagtt tgcgggacaa
     4321 atgactggtg ttgaaggtta tgtagaaagc gcagctagcg gcttagttgc aggtatcaat
     4381 cttgcgcata aaatattagg caaaggtgag gtagtatttc cgagagaaac tatgattgga
     4441 agtatggctt actatatttc tcatgctaaa aacaataaga atttccaacc tatgaatgct
     4501 aacttcgggt tattaccatc tttagaaact agaattaaag ataaaaaaga acgctatgaa
     4561 gcacaagcta atagagcttt ggattactta gaaaatttca aaaaaacttt ataaaatagt
     4621 tagaaagact agatatgcta ttcattctta agtcatcaac gagtaagtaa tgactttcta
     4681 aatggaaaat acttatccta gtctttttaa ttttggaatt gttacgtatt tctgacaatt
     4741 tagaattcgt attcaaaaaa atatctaaat aaataac
//
LOCUS       DAHHSC010000178         4717 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.179, whole genome shotgun sequence.
ACCESSION   DAHHSC010000178 DAHHSC010000000
VERSION     DAHHSC010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 4717)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4717)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4717
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.179"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            74..1258
                     /locus_tag="NBI57_002187"
     CDS             74..1258
                     /locus_tag="NBI57_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HCD1823116.1"
                     /translation="MSEKQQILDYIETNKYSYIEISHRIHERPELGNEEIFASRTLID
                     RLKEHDFEIETEIAGHATGFIATYDSGLDGPAIGFLAEYDALPGLGHACGHNIIGTAS
                     VLGAIGLKQVIDQIGGKVVVLGCPAEEGGENGSAKASYVKAGVIDQIDIALMIHPGNE
                     TYKTIDTLAVDVLDVKFYGKSAHASENADEALNALDAMISYFNGVAQLRQHIKKDQRV
                     HGVILDGGKAANIIPDYTHARFYTRAMTRKELDILTEKVNQIARGAAIQTGCDYEFGP
                     IQNGVNEFIKTPKLDDLFAKYAEEVGEAVIDDDFGYGSTDTGNVSHVVPTIHPHIKIG
                     SRNLVGHTHRFREAAASVHGDEALIKGAKIMALMGLELITNQDVYQDIIEEHAHLKGN
                     GK"
     gene            1258..2451
                     /locus_tag="NBI57_002188"
     CDS             1258..2451
                     /locus_tag="NBI57_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HCD1823117.1"
                     /translation="MTNNGNEPNLTLSDLYDKDVVYTSRPSYISNPWLKPDEHQSNFL
                     TGRELLIANQLPVIVHEASATDKLHQLFQVIGKEVPNSIFTFNNQQSYENLIKQLAHK
                     ENKKIYFQYIHDETILNQQYYALDKTLFVALNNKARIPEWTNGKFLPKRKVVKIEQFE
                     NEIKNWEFPLVIKPGDDLPTAGGYGVMICYHDADLQKAITRIKEATAETNSLIIEQKI
                     EEKANYCVQFAYSESLGIQYLGAATQLTDKYGFYNGNENTTNVPEHVIEAGRQIMENG
                     VNQGFFGVAGFDLLVDEDDNVYAIDLNFRQNGSTSMLLLANELNSGYQKFYSYHSKGD
                     NTHFFNTILKYVKEGSLYPLSYYDGDWYGEDKVKSRFGCIWHGDSKETVLENERAFLA
                     ELEHY"
     gene            2850..3521
                     /locus_tag="NBI57_002189"
     CDS             2850..3521
                     /locus_tag="NBI57_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2750 domain-containing protein"
                     /protein_id="HCD1823118.1"
                     /translation="MSYKAYPFFRDILINECIYFASKNKKLVRLNYKSEAYVGVWTEE
                     SVAVSFLTSRDIPFDKVVKMDVDRFATYELDELFDEQDHIIMNQTMEEEGHLLNVVAV
                     TQEVMTELDKIRIKEFVQDVAKYDEVYGLTKKGSKQFILISENDSDEKKPHIMPVWSI
                     KNRALKVRDEDFEECDLITIEGSVFGEWLDELRDDHKAVAIDLKTGVVGTIVSAQKLS
                     NELTF"
     gene            complement(3652..4455)
                     /gene="coaW"
                     /locus_tag="NBI57_002190"
     CDS             complement(3652..4455)
                     /gene="coaW"
                     /locus_tag="NBI57_002190"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II pantothenate kinase"
                     /protein_id="HCD1823119.1"
                     /translation="MKVGIDAGGTLIKIVQEQDNQRTFKTELTKNIDQVVEWLNQQQI
                     EKLCLTGGNAGVIAENINIPAQIFVEFDAASQGLGILLKEQGHDLADYIFANVGTGTS
                     LHYFDGQSQRRVGGIGTGGGMIQGLGYLLSQITDYKQLTDMAQHGDRNTIDLKVRHIY
                     KDTEPPIPGDLTAANFGHVLHHLDADFTPSNKLAAVIGVVGEVVTTMAITVAREFKTE
                     NIVYIGSSFHNNALLRKVVEDYTVLRGCKPYYVENGAFSGAIGALYLGK"
BASE COUNT         1629 a          660 c          944 g         1484 t
ORIGIN      
        1 ataattcatt ttgaaagaac aaaatattgt ggatgattaa aatgagtgta gggtaaaagg
       61 gagatagtaa aaaatgagtg aaaaacaaca aattctcgat tatatagaaa caaataaata
      121 tagttatatc gaaatcagtc atagaattca tgaacgtcct gaacttggta atgaagaaat
      181 atttgcgtct cgaactttaa tagatcgttt gaaagagcat gattttgaaa ttgaaaccga
      241 gattgctggg catgcaactg ggtttatagc gacatatgat tcaggtcttg acgggccagc
      301 tataggtttt ttagcagaat acgatgcttt gccagggttg ggtcatgctt gtggtcataa
      361 tatcattgga actgcaagtg tgcttggtgc aattggtttg aagcaagtga ttgaccaaat
      421 tggtggtaaa gtagtcgttc ttggatgtcc agctgaagaa ggtggggaaa atggtagcgc
      481 taaagcttct tatgtcaagg ctggtgtgat tgatcaaata gacattgcct taatgattca
      541 tccgggaaat gaaacttata aaacgattga tactttggca gtggatgttt tagatgttaa
      601 attttacgga aaaagtgctc atgcctctga aaatgcagat gaagcgttaa atgcattaga
      661 cgctatgatt agttatttta atggtgtagc acaactacga caacatatta aaaaagatca
      721 acgtgtgcat ggtgtgattt tagatggcgg gaaagcagct aatattattc cagactatac
      781 acatgctcgt ttttatacta gagcaatgac gcgtaaagaa ttggatatat taacagaaaa
      841 agtaaatcaa atcgcacgtg gagctgcgat acagactggt tgtgattatg aatttggtcc
      901 aattcaaaac ggtgtgaatg aattcattaa aacgccgaaa ttagatgatt tatttgctaa
      961 atatgctgaa gaagttggtg aagcagttat tgatgatgat tttggttatg gctctacgga
     1021 tacagggaac gtaagtcatg ttgtgccaac aatacatcct catattaaaa taggatcacg
     1081 taatttagta ggacatacgc atagatttag agaggcggct gcgagtgtac atggtgatga
     1141 agcattaatt aaaggtgcta aaataatggc gttgatgggg ttagagttaa ttacaaatca
     1201 agacgtttat caagacatta ttgaagagca tgcgcatttg aaagggaatg ggaagtaatg
     1261 acaaataatg gtaatgagcc gaacttaacg ttaagtgatt tatatgacaa agatgttgtc
     1321 tatacgtcaa gaccttcgta tatatcgaat ccatggttga aaccggacga acatcaatct
     1381 aattttttaa ctggtaggga gttattaata gcaaatcaat tacctgttat tgttcacgaa
     1441 gctagtgcga cagacaaatt gcatcagcta tttcaagtta ttggtaagga agtaccgaac
     1501 tcaattttta catttaacaa tcagcaatct tacgaaaact tgatcaaaca attggcacat
     1561 aaggaaaata aaaagattta ttttcaatat attcatgatg aaacaatctt gaatcagcaa
     1621 tattatgcat tagataaaac gttatttgtt gctttaaata ataaagcaag aatacctgaa
     1681 tggacaaatg ggaagttttt accaaaaaga aaagttgtaa aaattgagca attcgaaaac
     1741 gagattaaga attgggaatt cccgttagta attaagccgg gagatgatct tccgacagca
     1801 ggtggctatg gcgttatgat ttgttatcat gatgcagatt tacaaaaagc gataacgcgt
     1861 attaaagagg caactgctga aacgaattcg cttattatcg aacagaagat tgaagaaaaa
     1921 gcgaattatt gcgttcagtt tgcatactcg gaatcgctag gcattcaata tcttggtgcg
     1981 gcaacgcaat taacggataa gtacggattt tataacggaa atgaaaatac tactaatgta
     2041 ccagagcacg ttatagaagc aggtagacaa attatggaaa acggtgtgaa tcaaggattc
     2101 tttggtgtag ctggttttga cctactcgtc gatgaggatg ataacgttta tgcgattgat
     2161 ttaaacttta gacaaaatgg ttcaacgagc atgttattac ttgctaacga gttgaattca
     2221 ggatatcaaa agttttatag ttatcattca aaaggtgata acacacattt cttcaatacg
     2281 attttgaaat atgtcaaaga aggtagttta tacccgttat cttattatga tggtgattgg
     2341 tacggtgaag ataaagttaa atcaaggttt ggctgtattt ggcatggtga ttcaaaagaa
     2401 acagtactgg agaatgaacg cgcattttta gctgaacttg aacactatta gagttcggaa
     2461 cataaggcgc tacaatgttg tgttgccagt agttgactga atatgcgttt gtaacaagct
     2521 tttttcgatt ctagtcaaca gtaattaaat ttatgatatg gcaatacttt gtaatactaa
     2581 tattaaatgg cgacttttat ttcactatgt tataagagtt gccattttgt tgataaaggt
     2641 atactaaagg ttatcgtttt gaaattttta gtaactagat atgtttcgtg ttatagaccg
     2701 aatttgtgta tacgtaaaat ttaatgctat tgaattttta aaatgaaaaa catgacatta
     2761 aattgaattc ataatatgtc taattgacta acttgttgga gtcatttact attttatgta
     2821 tgacatattt taaaaagtga gggtcaagca tgtcttataa agcatatcca ttctttagag
     2881 atatattaat aaatgaatgt atttatttcg cctctaaaaa taaaaaacta gtacgcctaa
     2941 attataaaag tgaagcgtat gtaggcgttt ggacagaaga aagtgtggcc gtatcatttt
     3001 taacaagtcg tgatattcca tttgataaag ttgtaaaaat ggacgttgat cgttttgcta
     3061 cttatgaatt agatgaattg tttgatgaac aagaccatat tattatgaat caaacaatgg
     3121 aagaagaagg gcatctacta aacgttgtag ctgttacaca agaagtgatg acggaattag
     3181 ataaaattag aatcaaagaa tttgtccaag atgtagcaaa atatgatgaa gtatacggct
     3241 taactaaaaa aggtagtaag cagtttattc tcattagtga aaatgatagc gacgaaaaaa
     3301 agccgcatat tatgcctgta tggagtatta aaaacagagc gttaaaagtt cgagatgaag
     3361 attttgaaga gtgtgattta attacgattg aaggttctgt tttcggagaa tggctagatg
     3421 aacttagaga tgatcataaa gccgttgcaa tagatttaaa aactggcgtg gttggtacaa
     3481 ttgtttcagc gcaaaaattg tctaatgaac ttacatttta attgaattga gttgtaagta
     3541 attaagtgat aataaatgga acaatacgta ttccaaaaca cgtagaccat aaaataaaag
     3601 gccatttata tagcgtttat ttaaaacaac gcgcatataa atggtctttt tctatttacc
     3661 taaatataat gcaccaatag cacctgaaaa tgcgccgttt tcaacatagt acggtttgca
     3721 accgcgtaac acagtataat cttccacaac tttgcgtaat aaagcgttat tatggaatga
     3781 agaaccgata taaacgatat tttcagtttt aaattcacgt gcaacagtaa tggccattgt
     3841 cgtaacaact tcgccaacga caccaataac ggctgctaat ttattgctag gtgtaaaatc
     3901 agcatctaaa tgatgtagta catgaccaaa attagctgct gttaaatcac cgggaatggg
     3961 tggttcggta tctttataaa tatgtctaac ctttaaatcg atagtgttac gatcaccgtg
     4021 ttgtgccatg tcagttaact gtttataatc agtgatttga cttagtaaat aaccgagtcc
     4081 ttgaatcatg cctccacctg taccgatacc gcctacacga cgttgtgatt ggccgtcgaa
     4141 ataatgtagt gacgtaccgg taccaacatt tgcaaaaata taatctgcta agtcatggcc
     4201 ttgctctttt aacaaaatac ctagtccttg agatgcagca tcaaactcta caaaaatttg
     4261 tgcaggaatg ttgatgtttt cagcaatgac acctgcatta cctccagtta agcataattt
     4321 ttcaatttgc tgttggttta accattccac aacttgatca atatttttag ttaattcagt
     4381 tttaaaagta cgttggttat cttgctcttg aacgatttta attagtgtac cgccagcgtc
     4441 aatgccaact ttcataagat tcccacctca ttattaatgt ctatccttaa ataatagtat
     4501 agtaaaatga ctaaaaaaca agtaataata gtaattatta acaaatttga tgccattgta
     4561 tttcgatatt gtaagcgcat cgcaattaat gtattacaaa cgtagacgtt aagttatata
     4621 tattattttc taggaatttt gaagttgtat aggattgtta gttagtgacg caatattaaa
     4681 agtagttcgt acgcagtgta tttgtaagtc tctgatt
//
LOCUS       DAHHSC010000179         4663 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.180, whole genome shotgun sequence.
ACCESSION   DAHHSC010000179 DAHHSC010000000
VERSION     DAHHSC010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 4663)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4663)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4663
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.180"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            <1..270
                     /locus_tag="NBI57_002191"
     CDS             <1..270
                     /locus_tag="NBI57_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000669709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAP domain-containing protein"
                     /protein_id="HCD1823120.1"
                     /translation="NKFENIVSNEFVFYNASKITINDLSIKLKSAMANDQGITKHDIG
                     LAERAVYKVYFKNGSSKYVDLKTEYKDERVFKATDIKKVDIELKF"
     gene            complement(549..1835)
                     /locus_tag="NBI57_002192"
     CDS             complement(549..1835)
                     /locus_tag="NBI57_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HCD1823121.1"
                     /translation="MNPLAQSLNEQLQQSNATAFTMLSDLGQNMFYPKGILSQSAEAK
                     STTYNATIGMATNKDGKMFASSLDAMFNDLTPDEIFPYAPPQGIEELRDLWQQKMLRD
                     NPELSIDNMSPPIVTNALTHGLSLVGDLFVNQGDTILLPEHNWGNYKLVFNTRNGANL
                     QTYPIFDKDGHYTTDSLVEALQSYNKDKVIMILNYPNNPTGYTPTHKEVTTIVDAIKA
                     LADKGTKVIAVVDDAYYGLFYEDVYTQSLFTALSNLNSNAILPVRLDGATKEFFAWGF
                     RVGFMTFGTSDQTTKEVLEAKVKGLIRSNISSGPLPTQSAVKHVLKNNKQFDKEIEQN
                     IQTLKERYEVTKEVVYADQYHSHWQAYDFNSGYFMAIKVHDVDPEELRKHLIEKYSIG
                     VIALNATDIRIAFSCVEKEDIPHVFDSIAKAIDDLR"
     gene            2035..2133
                     /locus_tag="NBI57_002193"
     CDS             2035..2133
                     /locus_tag="NBI57_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1823122.1"
                     /translation="MMWWQEGMMTLITGGLLIIFRLWLELKWKNKK"
     gene            2376..2552
                     /locus_tag="NBI57_002194"
     CDS             2376..2552
                     /locus_tag="NBI57_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1823123.1"
                     /translation="MKHLTKIFVVLAIILFIIGYYLQATNHESQGIKLLLAAIMFMIC
                     AFISRSNDRRKNSK"
     gene            2810..3190
                     /locus_tag="NBI57_002195"
     CDS             2810..3190
                     /locus_tag="NBI57_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCD1823124.1"
                     /translation="MKIILKNNSDFPIYEQIKQQVKQNILKGHVAPGEHLPSMRELAK
                     DLQVSLITTKRAYEDLEKDGFVTTIRGKGTFVKEQDSSILKEKQFFTIENLVKELVNE
                     AQAIEMSLEELQDILTFIYEEESS"
     gene            3187..4083
                     /gene="pmtA"
                     /locus_tag="NBI57_002196"
     CDS             3187..4083
                     /gene="pmtA"
                     /locus_tag="NBI57_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenol-soluble modulin export ABC transporter
                     ATP-binding protein PmtA"
                     /protein_id="HCD1823125.1"
                     /translation="MNAIELSNVNYSSDQFNLKNISFKVPQGFVTGFIGRNGAGKTTI
                     IRLIMDLYQPQTGVIRVLEEDMALNPIELKNSIGFVYSENYFNERWTTKQLEKMIAPF
                     YRKWDHQVFEFYLEKFDLPINKSIKTFSTGMKMKLSLAVAFSHHAELYIFDEPTSGLD
                     PLARNELLEIIQQELIDENKTIFMSTHIISDLEKIADYIIHLSDGEVILNGSKEQLLQ
                     RYQVVSGATEDLDDELASLLIYEEHKRTGFIGLTEHAQVFKEILGHKVNITTPSIENL
                     MVYLEKRKPKYHENIKLMEEGF"
     gene            4084..>4663
                     /gene="pmtB"
                     /locus_tag="NBI57_002197"
     CDS             4084..>4663
                     /gene="pmtB"
                     /locus_tag="NBI57_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenol-soluble modulin export ABC transporter
                     permease subunit PmtB"
                     /protein_id="HCD1823126.1"
                     /translation="MKALLIRNFKLRRYTLIIYVLLLTLYPFYIMLDSTKFFYLLQSF
                     ISPTILIIWILDAGHLFRLNRRLGGNDSYYFYMSLPVSKKQLLNANYITCIVLTLIGT
                     LVISLYAYEADVIEPNSIYFSTAYAFVISNFLSIPIAFSQFTELRRVKVPYGIYVFTI
                     IILVPFLFSIAIVLVNYFVLSQSSFPDLYSYIL"
BASE COUNT         1598 a          630 c          794 g         1641 t
ORIGIN      
        1 aataaatttg aaaacatagt ttcaaatgaa tttgtgttct ataatgcaag caaaattaca
       61 attaatgatt taagtataaa acttaaatca gcaatggcaa atgatcaagg gataactaaa
      121 catgacatag gacttgctga acgcgcagtg tataaagtgt attttaaaaa tggttcgtca
      181 aaatatgtag acttaaaaac tgagtataaa gatgaaagag tatttaaagc aactgacatt
      241 aaaaaggtag atattgaact taaattctaa tctttaattt tataataagg catctcacaa
      301 tcgtggggtg ccttttacat ttgtagagat gtgatgcttg aatgtttgaa gtgatttgcc
      361 aaaagttata taaatttgta ccaaataaat taaattttgt taggagagat gtataaaact
      421 aattaggtat agtgtttgac aagttgctga ataaaatata tcttatgata ctttgaaaga
      481 aggaataatt ttaaaaataa aaaaccaata atccgagtca tactcatcag attattggtt
      541 gaaattaatt atcttaagtc atcaatcgct ttagcaattg aatcaaatac atgtgggata
      601 tcttcttttt ctacacagct aaacgcaata cgaatatctg ttgcattaag tgcaataaca
      661 ccaattgaat atttttcaat taaatgttta cgaagttctt cagggtcaac atcatgaact
      721 tttattgcca taaagtatcc agagttaaag tcataggctt gccaatgtga atgatattga
      781 tcagcataga caacttcctt agtgacttca tagcgttctt ttaatgtttg aatattttgt
      841 tcgatttctt tatcgaattg tttattattt tttaatacat gcttaacagc gctttgtgtt
      901 ggaagaggtc cactagaaat gttacttcgt ataagacctt ttactttggc ttctaatact
      961 tctttagttg tttgatcaga cgttccaaat gtcataaacc caacacggaa tccccatgcg
     1021 aaaaattctt ttgttgcacc atctaaacga acaggtaata ttgcatttga atttaaatta
     1081 gataatgcag taaataatga ttgagtatac acatcttcat agaataaacc atagtatgcg
     1141 tcatccacaa cagctataac ttttgtacct ttatcagcta atgctttaat tgcatcgacg
     1201 atggtagtca cttctttatg cgtaggtgtg taacctgtcg gattattagg ataatttaaa
     1261 atcataatga ctttatcttt attgtatgat tgtaaagctt ctacaagtga atcagtagta
     1321 taatgcccgt ctttatcaaa gataggatat gtttgaaggt ttgcaccatt tctcgtattg
     1381 aaaacaagtt tgtaattacc ccaattatgc tctggtaaca agatagtgtc accttgattt
     1441 acaaataaat cgccaactaa agataaacca tgtgttaatg cattcgtaac aattggtggt
     1501 gacatgttgt cgattgatag ctctggattg tcacgcaaca ttttttgttg ccataaatca
     1561 cgtaattctt cgatgccttg tggtggcgca taagggaata tttcatctgg agttaaatca
     1621 ttaaacattg catctaaaga tgatgcaaac atttttccgt ctttgtttgt cgccatacct
     1681 atagttgcat tatatgttgt actctttgct tcagcagatt gagataaaat gccttttgga
     1741 taaaacatat tttgacctaa gtcagataac attgtaaagg cagttgcatt tgactgttga
     1801 agttgttcat ttaagctttg ggctaaagga ttcattattg cttaacctct ctttaaaaaa
     1861 attgtatgcc tatatattat ctttaattat accaaagata aagtgttgtt ggtgaattaa
     1921 cgaataatat gtaaaatctg ctgccaaaat acgaacatat gttctgtttt tatgttagaa
     1981 taggtgtagg tcatccttca gtatgatact ttagtcgaac ggaggtgtag tgtaatgatg
     2041 tggtggcaag aagggatgat gacacttatt acaggtggct tactaattat ttttcgactt
     2101 tggttagaac tgaaatggaa aaataaaaaa tgatcctaca cacataaata accccaagtc
     2161 tgctggtacc agacttgggg ttgtgtagta taattctgtg gatacactta aataatatca
     2221 ttgaacacaa tatatcgcaa tcaataatca ttttgattga atggttttgt tgcgctattt
     2281 tagttgaaag tgattagtac cttgaacttt tgaaattcaa taacttatta aaaaaattta
     2341 tatttgttat aatgtcgatg ttgaggtgaa ataaattgaa acatctaaca aaaatatttg
     2401 tagtacttgc aattatttta tttattattg gttattattt acaagcaact aatcatgaaa
     2461 gccaaggtat aaaattatta ttagcagcga ttatgtttat gatttgtgct tttataagta
     2521 gaagtaatga tcgacgtaaa aatagtaaat agaattgact gtttaaaacg attgtttatg
     2581 ttgacgctac ggttgtcacg tagagattaa agacattcgt tttagcagtc ttttttattg
     2641 aagtagacaa tgcaagatta aatggtagtg tcatttcatt ttattcaact gctagtaata
     2701 caatactcag tgttatttct gaagactggt tgataatgga catgcatatt tacaaaactg
     2761 tgtatattgt gtatattgta tatatacaga agttgatagg gggacgttga tgaaaataat
     2821 tttaaaaaac aatagtgatt ttccgattta tgaacagatt aagcaacaag taaaacaaaa
     2881 tattttaaag ggacatgttg ctcctggaga gcatttgccg tcaatgagag aacttgccaa
     2941 agatcttcaa gtaagtttga ttactaccaa acgtgcttat gaagatttag agaaagacgg
     3001 ttttgttaca acaattagag gaaaagggac ctttgttaag gagcaagata gttctatttt
     3061 aaaagagaaa caatttttta ccattgaaaa tttggttaaa gaattggtta atgaagcgca
     3121 agccatcgaa atgtcacttg aggaacttca agatatttta acgttcattt atgaggagga
     3181 atcatcatga atgccataga attaagtaat gttaattatt caagtgatca gtttaattta
     3241 aagaatatct ctttcaaagt tcctcaagga ttcgtcactg gttttattgg tagaaatggc
     3301 gctggtaaaa ccacaataat aaggttaatt atggatttgt atcaaccaca aactggtgtt
     3361 attagagtat tagaagagga tatggctctc aatccgatag agttaaagaa tagtattggg
     3421 tttgtttact ctgaaaatta ttttaatgag agatggacta cgaagcaact tgaaaaaatg
     3481 attgcccctt tttatcgtaa atgggaccat caagtatttg aattttatct tgaaaagttc
     3541 gatttaccaa tcaataaatc aataaaaaca ttttcgactg ggatgaaaat gaaattatca
     3601 ctagcagttg cattttcaca tcatgctgaa ttgtatattt ttgatgagcc tacttcaggt
     3661 cttgatccct tagctagaaa tgagctattg gagataattc agcaagaatt aattgatgaa
     3721 aataaaacaa tattcatgtc gacacacatt atctcagatt tagaaaaaat agctgattat
     3781 atcattcact taagtgacgg tgaagttatc ttaaatggtt caaaagaaca actgttacaa
     3841 agatatcaag ttgtgagtgg tgcaactgag gaccttgatg atgaattagc atctctactt
     3901 atttatgaag aacataagcg tacgggattt ataggattaa cagaacatgc gcaagtattt
     3961 aaagaaattt taggtcataa agtgaatatc actacacctt ctattgagaa cttaatggtt
     4021 tatcttgaaa agagaaaacc taaatatcat gaaaacatca aattgatgga agaaggtttt
     4081 taaatgaagg cattgttaat taggaatttt aaattacgac gatatacact tatcatctat
     4141 gttctattat taaccttata tcctttttat ataatgcttg actcaacaaa gttcttttac
     4201 ctattgcaat cttttataag tccaacaata ttgattattt ggattttaga tgctggtcac
     4261 ttatttagac tcaatcgtag attaggtggt aatgattcat attattttta tatgagttta
     4321 ccagtgtcaa aaaaacaatt gcttaatgcc aactatatta catgcattgt tttaacatta
     4381 attggaacgc ttgttattag tttatatgct tatgaagcag atgtgattga accaaattca
     4441 atctattttt caactgcata tgcatttgtc atatctaact tcttgtctat accaattgca
     4501 tttagtcaat ttacagaatt gcgtagagtc aaagtgccat atggtatata cgtgtttact
     4561 attatcattt tagttccatt tttattttca attgcaatag tgttggtgaa ttattttgtt
     4621 ctaagtcaat catcattccc agatttatat tcatatattt taa
//
LOCUS       DAHHSC010000180         4654 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.181, whole genome shotgun sequence.
ACCESSION   DAHHSC010000180 DAHHSC010000000
VERSION     DAHHSC010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 4654)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4654)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4654
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.181"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            79..1062
                     /locus_tag="NBI57_002198"
     CDS             79..1062
                     /locus_tag="NBI57_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HCD1823127.1"
                     /translation="MKKWQFVGTTALGATLLLGACGGGNGGSGNSDLKGEAKGDGSST
                     VAPIVEKLNEKWAQDHSDAKISAGQAGTGAGFQKFIAGDIDFADASRPIKDEEKQKLQ
                     DKNIKYKEFKIAQDGVTVAVNKENDFVDELDKQQLKAIYSGKAKTWKDVNSKWPDKKI
                     NAVSPNSSHGTYDFFENEVMNKEDIKAEKNADTNAIVSSVTKNKEGIGYFGYNFYVQN
                     KDKLKEVKIKDENGKATEPTKKTIQDNSYALSRPLFIYVNEKALKDNKVMSEFIKFVL
                     EDKGKAAEEAGYVAAPEKTYKSQLDDLKAFIDKNQKSDDKKSDDKKSEDKK"
     gene            1253..2179
                     /gene="pstC"
                     /locus_tag="NBI57_002199"
     CDS             1253..2179
                     /gene="pstC"
                     /locus_tag="NBI57_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HCD1823128.1"
                     /translation="MTSSTNVKALIEKNINKKGKHNDKIIPVILAAISAISILTTLGI
                     LITLLLETITFFTRIPITEFLFSTTWNPTGSDPKFGIWALIIGTLKITVIATIFAVPV
                     GLGAAIYLSEYASDRARRIIKPILEILAGIPTIVFGFFALTFVTPVLRSFISGLGEFN
                     AISPGLVVGIMIVPLITSLSEDAMASVPNKIREGAYGLGATKLEVATKVVLPAATSGI
                     VASIVLAISRAIGETMIVSLAAGSSPTASLSLTSSIQTMTGYIVEIATGDATFGSNIY
                     YSIYAVGFTLFIFTLIMNLLSQWISKRFREEY"
     gene            2181..3098
                     /gene="pstA"
                     /locus_tag="NBI57_002200"
     CDS             2181..3098
                     /gene="pstA"
                     /locus_tag="NBI57_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HCD1823129.1"
                     /translation="METTDNNRQSLVDQQLVQKHLSSRTVKNKVFKLIFLACTLLGLV
                     VLIALLTQTLIKGVSHLNLQFFTNFSSSTPSMAGVKGALIGSLWLMLSIIPLSIILGI
                     GTAIYLEEYAKNNKFTQFVKISISNLAGVPSVVFGLLGYTLFVGGAGIEALKMGNSIL
                     AAALTMTLLILPIIIVSSQEAIRAVPNSVREASYGLGANKWQTIRRVVLPAALPGILT
                     GFILSLSRALGETAPLVLIGIPTILLATPRSILDQFSALPIQIFTWAKMPQEEFQNVA
                     SAGIIVLLVILILMNGVAIILRNKFSKKF"
     gene            3145..3996
                     /locus_tag="NBI57_002201"
     CDS             3145..3996
                     /locus_tag="NBI57_002201"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HCD1823130.1"
                     /translation="MAQTLAQTKQISQSHTFDVSQSHHKTPDDTNSHSVIYSTQNLDL
                     WYGENHALQNINLDIYENQITAIIGPSGCGKSTYIKTLNRMVELVPSVKTAGKILYRD
                     QDIFDQKYSKEQLRTNVGMVFQQPNPFPKSIYDNITYGPKIHGIKNKKVLDEIVEKSL
                     RGAAIWDELKDRLHTNAYSLSGGQQQRVCIARCLAIEPEVILMDEPTSALDPISTLRV
                     EELVQELKEKYTIIMVTHNMQQAARVSDKTAFFLNGYVNEYDDTDKIFSNPSNKKTED
                     YISGRFG"
     gene            4003..4644
                     /gene="phoU"
                     /locus_tag="NBI57_002202"
     CDS             4003..4644
                     /gene="phoU"
                     /locus_tag="NBI57_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HCD1823131.1"
                     /translation="MAIIRQRYQEQLDDLIKELRRLGANVYVSIENGIKSLSIDDRGF
                     ARQTVKNDKHINQLNYDINERVIMLITKQQPIASDLRMMISSLKIASDLERIGDNASS
                     IANIRLRTKITDDYVLTRLKTMGKLAMLMLKDLDQAFKKKDTVLIREIIERDEDIDDL
                     YSHIINATYLIDNDPFVAAQAHLAARHLERIGDHIINIAESVYFYLTGTHYEQ"
BASE COUNT         1687 a          698 c          841 g         1428 t
ORIGIN      
        1 aggttaataa ttcaaggtta agataaagat gtaatcaata caaatactat ttgttgttca
       61 tacagggagg atatttcaat gaaaaaatgg caatttgttg gtactacagc tttaggtgca
      121 acactattat taggtgcttg tggtggcggt aatggtggca gtggtaatag tgatttaaaa
      181 ggggaagcta aaggtgatgg ctcatcaaca gtagcaccaa ttgtggagaa attaaatgaa
      241 aaatgggctc aagatcactc ggatgctaaa atctcagcag gacaagctgg tacaggtgct
      301 ggtttccaaa aattcattgc aggagatatc gacttcgctg atgcttctag accaattaaa
      361 gatgaagaga agcaaaaatt acaagataag aatatcaaat acaaagaatt taaaattgcg
      421 caagatggtg taacggttgc tgtaaataaa gaaaatgatt ttgtagatga attagacaaa
      481 cagcaattaa aagcaattta ttctggaaaa gctaaaacat ggaaagatgt taatagtaaa
      541 tggccagata aaaaaataaa tgctgtatca ccaaactcaa gtcatggtac ttatgacttc
      601 ttcgaaaatg aagtaatgaa taaagaagat attaaagcag aaaaaaatgc tgatacaaat
      661 gctatcgttt cttctgtaac gaaaaacaaa gagggaatcg gatactttgg atataacttc
      721 tacgtacaaa ataaagataa attaaaagaa gttaaaatca aagatgaaaa tggtaaagca
      781 acagagccta cgaaaaaaac aattcaagat aactcttatg cattaagtag accattattc
      841 atttatgtaa atgaaaaagc attgaaagat aataaagtaa tgtcagaatt tatcaaattc
      901 gtcttagaag ataaaggtaa agcagctgaa gaagctggat atgtagcagc accagagaaa
      961 acatacaaat cacaattaga tgatttaaaa gcatttattg ataaaaatca aaaatcagac
     1021 gacaagaaat ctgatgataa aaagtctgaa gacaaaaaat aataagacgc aatttcaaat
     1081 gtgtcttgaa acatgatttt gatggtgaat cattatttag agtacaaagc ttgatttatc
     1141 gagacgctga ttttgacatt cagttagtct acaagcttat caacttaaaa tagtggttca
     1201 tcattatttt acaaatctaa ttattttggg agtaatagaa agaggtttga ttatgacttc
     1261 atctactaat gttaaagctt taatcgaaaa aaatattaat aaaaaaggga agcataatga
     1321 caaaattata ccagttattt tagccgcaat ttcagcgatt tccattttaa caacactagg
     1381 tatattaatc acattgcttt tagaaaccat cacttttttc accagaattc caataactga
     1441 atttctattt tctactactt ggaatcctac cggttcagac cctaagtttg gtatctgggc
     1501 attgataata gggactttaa aaatcacagt tattgcgact atatttgcag ttccagtcgg
     1561 gcttggtgca gcaatttatt taagtgaata tgcaagtgac cgtgcacgac gcataattaa
     1621 accgatatta gaaattttag caggaatacc aacaattgtg tttggtttct ttgcattaac
     1681 ctttgttaca ccagtattaa gatctttcat atcaggtctt ggagagttta atgctataag
     1741 tcccggctta gttgtcggta ttatgattgt ccctctcatc acaagtttga gtgaggatgc
     1801 aatggcatct gtaccaaata aaattcgaga aggtgcctat ggacttggag caactaaatt
     1861 agaagtagca actaaagtcg tacttcccgc agcaacatca ggtattgtag cttcaatcgt
     1921 tctcgcgatt tcaagagcaa ttggagaaac gatgattgta tcattagcgg caggtagttc
     1981 gccaacagct tcattaagtt taacaagttc gattcaaaca atgactggat atattgttga
     2041 gatagcgaca ggtgatgcaa catttggatc aaatatttat tacagtattt atgctgtagg
     2101 gttcacacta tttatcttta ccttaatcat gaatttactt tctcagtgga tttctaagcg
     2161 ttttagggag gagtattaat atggaaacga cagataataa tagacaatca ctcgtcgatc
     2221 aacaacttgt ccaaaaacat ttatcatcca gaacggttaa aaataaagtg ttcaaactca
     2281 tatttttagc atgtacatta ttaggacttg tcgtacttat tgcgttgtta actcaaacat
     2341 tgattaaagg ggtaagtcat ttaaatttac agtttttcac taatttttct tcttcaacac
     2401 catctatggc tggcgttaaa ggcgcgttaa tcggttcact ttggttaatg ttaagtatca
     2461 ttccattatc aatcatccta ggaataggta cagctatata cttagaagaa tatgcgaaaa
     2521 acaacaaatt tactcagttt gttaaaatca gtatttccaa tttagctggt gtaccatcag
     2581 ttgtatttgg gttattaggt tacactttgt tcgttggtgg tgcggggatt gaagccttga
     2641 aaatgggtaa cagtatattg gcagcagcgc taacaatgac cttactgata ttaccaatta
     2701 ttattgtttc aagtcaggaa gcaattagag ctgtacctaa ctcagtacgc gaagcttctt
     2761 atggtttagg tgctaataaa tggcaaacga taagacgtgt tgtcttacca gcagcgttac
     2821 ctggtatttt aactggattc attttgtctc tttcaagagc actgggagaa acagcgccac
     2881 ttgtgctaat cggtataccg actatattat tggcaacacc tagaagtata ttggatcaat
     2941 tttcagcatt accaatccaa atatttactt gggcgaaaat gcctcaagaa gaattccaga
     3001 atgttgcatc ggcaggcatt atcgttttac tagttatctt aatcttaatg aatggcgttg
     3061 cgattatttt acgtaacaaa tttagtaaaa aattctaatt taaacaatca atctcattta
     3121 tctattaaaa agggagtttt aaatatggcg caaacacttg cacaaactaa acaaatatct
     3181 caaagtcata cgtttgatgt ctcacaaagt catcataaaa caccagatga tacaaactca
     3241 cattctgtta tatattcaac acaaaattta gacttatggt atggcgaaaa tcatgcatta
     3301 caaaatatta atttagatat ttatgaaaac caaattactg ccattatagg tccatctggt
     3361 tgtggcaaat caacatatat taaaactttg aatcgaatgg ttgagttagt accttctgtg
     3421 aaaacagctg gtaaaatatt atatcgagat caagacattt ttgatcaaaa atattctaaa
     3481 gaacaattac gtacaaatgt gggcatggtc tttcaacaac ctaatccatt tccaaaatca
     3541 atatacgata atattactta cggtccaaag attcacggta ttaaaaataa aaaagttctt
     3601 gatgaaatcg ttgagaaatc attacgtggc gctgcaattt gggatgaatt aaaggatagg
     3661 ttgcacacaa atgcatatag tttatccggt gggcaacaac aacgtgtttg tatcgcgcgt
     3721 tgtttagcaa ttgaacctga agtcatttta atggatgaac cgacatcagc attagatcca
     3781 atctcaacat taagagtaga agagttggtt caagaactaa aagaaaagta tacaattatt
     3841 atggttacac ataatatgca acaagcagct cgtgtatcag ataaaactgc atttttctta
     3901 aatggttatg tcaatgaata tgatgatact gataaaattt tctctaaccc atcaaacaag
     3961 aaaacagaag attatatttc aggaaggttt ggttgatata taatggcaat aattagacag
     4021 cgatatcagg agcaacttga tgatttaata aaggaattac gtcggttagg tgcaaatgtc
     4081 tatgtgagta ttgaaaatgg tataaaatca ttaagtattg acgatagagg ctttgcacga
     4141 caaacagtta aaaacgataa acatatcaat caattaaatt atgatattaa tgagcgagtt
     4201 atcatgttaa ttacaaagca acagcccatt gcgagtgatt tgcgtatgat gatttcttca
     4261 ttaaaaatcg cctccgattt agaaagaata ggagataatg catcgagtat tgccaatatt
     4321 cgattgcgta caaagattac agatgattat gtgttaaccc gtttaaagac aatgggtaaa
     4381 ttagctatgt taatgttaaa ggacttagat caagcattta aaaagaaaga taccgtatta
     4441 ataagagaaa taattgagcg tgatgaagat atcgatgact tatatagtca tattattaac
     4501 gcaacgtatc ttattgataa cgatccattt gtcgctgcac aagctcattt agcagcaaga
     4561 catttagaac gtattggtga tcatattatt aacatcgctg aaagtgttta tttttattta
     4621 acaggtacac attacgaaca ataacttaaa gtta
//
LOCUS       DAHHSC010000181         4654 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.182, whole genome shotgun sequence.
ACCESSION   DAHHSC010000181 DAHHSC010000000
VERSION     DAHHSC010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 4654)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4654)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4654
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.182"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            complement(762..1157)
                     /locus_tag="NBI57_002203"
     CDS             complement(762..1157)
                     /locus_tag="NBI57_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002502816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein"
                     /protein_id="HCD1823132.1"
                     /translation="MKDVKTDTHKAIEQLQTNQAELQQANNDYKATMDERIKHNETAV
                     KQYDQAFHRLTKGITAMFFIIALVMVTFLVLSPLGDWLGVQHFYEWLNYVLKTGHSAW
                     RYFILIFYLVPYALFGGLIYAILSVYKRI"
     gene            complement(1270..2280)
                     /locus_tag="NBI57_002204"
     CDS             complement(1270..2280)
                     /locus_tag="NBI57_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020366464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HCD1823133.1"
                     /translation="MATTKLSATKSTSRAINYAEKRAVEKSGLNCDVDYAKSSFKASR
                     ELYGKTDGNQGHVIIQSFKPGEVTPEQCNQLGLALAEKLAPNHQVAVYTHADTDHVHN
                     HIVMNSIDLETGKKFNNNKQALRNVRNFNDEVCMEHGLSVPEKDTARLRYTQTEKAIA
                     DPNTKSTAQYSWKDEIREAIDQSQATNMDEFKDHLNQYGIEIERVTPKSITYRHLAED
                     KKVRGRKLGEDYNKGGIEDGFERQIQRRRQQERDSDTECQPKRPTSSRDESTERDTGV
                     TQSDWDQFAQDTNELERSRQAAESARLADEKARRDREERERAKQASRRIINNDRDHGL
                     EL"
     gene            complement(2267..2464)
                     /locus_tag="NBI57_002205"
     CDS             complement(2267..2464)
                     /locus_tag="NBI57_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001836858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1823134.1"
                     /translation="MENTKGTMIVSAILLVVCLAIASFLIFHEQQIFPSLIFLGLAFI
                     DGTKFYLSYQKLKERDHHGDN"
     gene            complement(2440..2829)
                     /gene="mobC"
                     /locus_tag="NBI57_002206"
     CDS             complement(2440..2829)
                     /gene="mobC"
                     /locus_tag="NBI57_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016898877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="HCD1823135.1"
                     /translation="MSEQNTFMASDETVGRNRKPNRKAPKQISFRVSESEYLKLQQSA
                     ETLNMSVPAFVKKKAQGARLVAPKLDQATRQSVAKDLSMLGANANQIAKYCNQHQHEA
                     PNYEALERNISELRERLDEVWKTLKEQ"
     gene            complement(3099..3377)
                     /locus_tag="NBI57_002207"
     CDS             complement(3099..3377)
                     /locus_tag="NBI57_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002447416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1823136.1"
                     /translation="MLLRETEDYEKYYKIGNVVMGTAFICIVIFSLMKSTPLPDLSFL
                     KYIHFASEVLLIAGLIINSVPDFLTWNIKNIIFDLIIISFLIVFFIWV"
     gene            complement(3474..4274)
                     /locus_tag="NBI57_002208"
     CDS             complement(3474..4274)
                     /locus_tag="NBI57_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002498218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCD1823137.1"
                     /translation="MKSSLFILKEHFNNLYLMKRLAQFEMKISNKNNYLGTAWELINP
                     SIQIFMYWFIFGLGFRNNRTVENIPFIFWLIVGISMWFFVNQGILNGTKAVTSKYKQV
                     AKMNFPLSILSSYIVLSKFYTHVLLLILIMLICALNGYYPTIYTLQLLIFIPYTLLLT
                     MTIALLTSTLAVIIRDVQMLMQALMRIIFFISSILYLPTNTLVLKVIQFNPIYFIAES
                     YRAAILYQNWFFITHWKLSLYNLAFLLITFVLGSLLHVKYREQFADFL"
     gene            complement(4370..4576)
                     /locus_tag="NBI57_002209"
     CDS             complement(4370..4576)
                     /locus_tag="NBI57_002209"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1823138.1"
                     /translation="MRGRNEKISKLLIAFSLVTTLSYASASTGFMNDNSHEAFAKSKK
                     KKHKGNGNKHNCFPVGNSEFCTTP"
BASE COUNT         1481 a          812 c          718 g         1643 t
ORIGIN      
        1 caaagtaaaa aataaattaa cttcagtatt attaaatttt ctcaatggaa ctaaattcat
       61 atcattttta tatactactg tttctccact caactaactc cactccaatc taaaagaaac
      121 ctttatagaa taaagattcc tattaacttt aattgaaaat acttataata actattttat
      181 aaacaataat aacataaaga aacctaatat aaaaaaagtt tctttagttt aatgattggt
      241 tgcctttagt ttaatgattg gttgccttta gtttaatgat tggttgcctt tagtttaatg
      301 attggttgcc tttaacatct caaatccgtt gtcactctga ggatcaaatg cctcctaaaa
      361 aggttttaaa aagatttata aaaagatatt aaaaagttcg gggcaatttg cccctcacaa
      421 attcaaaaga caaaaaggaa accaaccaca cttgtggttg gtttttatta tttaaaaaag
      481 caataagtaa tataacttta atgcattatt ccaattgctt tattgacatt gagcctctga
      541 acccttaata aacccaaaaa ctgtcgactc gtcggtttca tagctcacgc tataccgaca
      601 ttcgtcgaaa cagtttaatt aagggttctt ctcaatgcac aataaatttt ctcggcataa
      661 tggcgtgtgt tactttttat ttctcttttc atttctaagc tattttaagc ttgatacagc
      721 ttttttatta cctcaaatgt ggtttttatt caacatttat attaaatgcg cttatacacg
      781 cttaaaatgg catatataag tcctccaaat aaagcataag gaacaagata gaaaataagt
      841 atgaaataac gccatgctga atgacctgtt ttcaggacat agttcagcca ttcataaaaa
      901 tgttgcacac ctaaccagtc gcctaaggga cttaagacta aaaatgtgac catgactaaa
      961 gcaataataa aaaacatcgc tgtaatgcct ttggttaaac gatgaaacgc ttgatcatat
     1021 tgtttaacag ctgtttcgtt gtgcttgata cgttcatcca tggttgcttt ataatcgtta
     1081 ttcgcttgtt gcagttctgc ttgattggtt tgtaactgct caatcgcctt atgtgtgtct
     1141 gtttttacat ctttcaattc ttcttggatg gctgtttgaa aatcttgttt taaattatcg
     1201 ttattaatgc gttcaatata gtgtttcgct acactgataa aacgcttttg tgtgatttca
     1261 gtcgcttgat taaagttcga ggccgtggtc tcgatcgttg ttaataattc gtcgtgacgc
     1321 ttgtttcgct cgttctctct cttctcgatc tcttcgagcc ttttcatcag caagtcttgc
     1381 tgattctgca gcttgtcggc tacgctcaag ctcatttgtg tcttgggcga attggtccca
     1441 atctgattga gtgacgcctg tatctcgttc agttgattca tcacgacttg aagttggtcg
     1501 ttttggctgg cattcggtgt ctgagtctcg ttcttgttgt ctccgtcgtt gaatttgtct
     1561 ttcaaagcca tcttcgatac ctcctttatt gtaatcttcg ccgagtttac gcccacgcac
     1621 ctttttatct tcggctaagt gtcgataggt aatgcttttg ggtgtgacac gttcaatttc
     1681 aataccatat tggtttaaat ggtctttgaa ttcatccata ttcgttgctt gtgattggtc
     1741 tatcgcctct ctaatctcat ctttccatga atattgggct gttgatttcg tattagggtc
     1801 agcaatcgct ttttctgtct gcgtataacg taagcgtgct gtgtcctttt ctggcacgct
     1861 taaaccatgt tccatgcaaa cctcatcatt gaaatttctg acattacgta acgcttgttt
     1921 attgttattg aatttcttac ctgtctcgag gtcaattgaa ttcatcacaa tatgattgtg
     1981 tacatggtct gtatctgcat gcgtatagac cgccacttga tgattcggtg cgagcttttc
     2041 agcgagtgct aagcctaatt gattacattg ttcgggcgtg acttcgccag gtttaaacga
     2101 ttggataatg acatgccctt gattcccatc ggttttacca tataactcac gagaagcttt
     2161 aaaactcgat ttggcatagt cgacatcaca atttaaacca cttttttcaa ccgcgcgttt
     2221 ttcagcataa ttaatggccc gtgacgttga tttagtcgca cttaatttag ttgtcgccat
     2281 gatgatcacg ttctttcaat ttttgataag acagataaaa tttagtgcca tcaataaaag
     2341 ctaatcctaa aaaaatcaaa cttggaaaaa tttgttgttc atggaaaatt aaaaaactgg
     2401 caattgctaa acaaacaact aataatatcg cacttacaat cattgttcct ttagtgtttt
     2461 ccatacctca tcaagccttt cacgtaattc actgatattg cgttctaatg cttcatagtt
     2521 cggtgcttca tgttgatgtt gattacaata tttggcaatc tgattggcat ttgcgcctaa
     2581 catactcaaa tcttttgcta ccgattgtcg cgttgcttga tccaatttgg gtgcgaccaa
     2641 tcgggcgcct tgtgcctttt tcttaacgaa agcaggcaca ctcatattta aagtttcagc
     2701 tgattgttgt aactttaaat attcggattc gctcacacga aaactgattt gtttcggagc
     2761 cttacggttg ggtttacggt ttcgcccaac agtttcgtcg ctagccataa atgtgttttg
     2821 ttcgctcatt caatcccacc acgctttctt cgaaagataa cgcctttctt acattttact
     2881 ttataggata aagtaaaatt aatcaagtcg ttaagtggtt tggcaagcat tggctttgct
     2941 agccactaac gtggcagacg cgccagcttg ttggggaata tccccaagcc ccttatgttt
     3001 ttgctaacgc aaaaaatgag tggcgaaatg ctagccactc atagttcaaa aaattcaaaa
     3061 taataattat agttaccaaa acatagttac tataattatt aaacccaaat gaaaaataca
     3121 attaaaaaag aaatgataat aaggtcaaaa atgatgttct ttatattcca agttaaaaag
     3181 tcagggacac tattaataat taaccctgct atcaataaaa cctcactagc aaagtgtatg
     3241 tactttaaaa aagaaagatc aggtaaagga gtgcttttca taagcgaaaa aataacaata
     3301 caaataaatg ctgttcccat aacaacattg cctatcttat aatatttttc atagtcttca
     3361 gtttctctta aaagcattta atcactccta agccgaaatc caatttgttt taactttaaa
     3421 ttttgtttga agtatatacc aaagtcttat ctttgagtgc ttttttatcc tgtttataaa
     3481 aaatctgcaa attgttcgcg atatttaaca tgtaaaagag agcccaacac aaaagtaatt
     3541 aaaaggaaag caagattata taaagatagt ttccaatgtg ttataaaaaa ccaattttga
     3601 tatagaattg ccgctctata actttcagct ataaaataga taggattaaa ttgtataacc
     3661 tttaaaacta aagtatttgt cggtaaatat aagattgaag atataaaaaa tattattctc
     3721 ataagagctt gcataagcat ttggacgtct ctaataatta cagctaatgt tgaagttaat
     3781 agtgctattg tcattgtcag taacagagtg taagggataa aaattaataa ttgtaatgta
     3841 tatatagtag ggtaatatcc atttaaagca catataagca taattaaaat aagcaataac
     3901 acatgggtat aaaatttact taagacgata taactagata atatagataa tggaaaattc
     3961 attttagcta cttgcttata tttgctagta actgcttttg taccatttaa aataccttga
     4021 ttcacaaaaa accacatact tattcctaca attaaccaaa aaataaaagg tatattctca
     4081 acagttctgt tatttctgaa acctaaacca aaaataaacc aatacataaa aatttgtatt
     4141 gaaggattaa ttaattccca tgctgttccc aaataattat ttttgttaga aattttcatt
     4201 tcaaattgtg ctaatctttt cattaaatat aaattattaa aatgttcttt taatataaac
     4261 aaagatgatt tcataacatt aagttacctt tctaagatta tcaatattat aaatgaaacc
     4321 taaaaaaaag gtaaagcaat tgtttattgc tttacctttt ctattttaat tatggagttg
     4381 tacaaaattc actgtttccg actgggaagc aattatgttt gttaccattt cctttatgtt
     4441 ttttcttttt agatttagca aatgcttcat gagagttatc attcataaaa ccagttgaag
     4501 cacttgcata tgatagtgtt gttactaatg aaaatgcaat aagaagtttc gagatttttt
     4561 catttctacc cctcaaaaat attaatttaa agtaaagata tcacaatttt aataaaaatt
     4621 aagacgaaaa aaatataaat ttattaattg gctt
//
LOCUS       DAHHSC010000182         4651 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.183, whole genome shotgun sequence.
ACCESSION   DAHHSC010000182 DAHHSC010000000
VERSION     DAHHSC010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 4651)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4651)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4651
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.183"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            complement(134..1000)
                     /locus_tag="NBI57_002210"
     CDS             complement(134..1000)
                     /locus_tag="NBI57_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HCD1823139.1"
                     /translation="MSHNENLKLAQRGAYLSLIVYIILSIVKYVTGFVFNSAAVRADA
                     LNNMTDIIVSLAVIIGLKISIKPADRNHPYGHLKSENISSLLVSFVIMFVGIQVVIQN
                     APRLFKEDDVVPNAITIIVSLISGLVMLIVFAVNQRLAKRTKSSSLNSAAKDNLSDSL
                     VSIGTAIGLIFTQIGFPIVDIILATLLGLLIVYTGFGIFKEAIFMLSDGFNETELEAY
                     RNDILEVDEVQEVKSIKGRYHGSSVFIDVTIVVDANLSLVEAHQICDNVEHHLHKKGI
                     SSVYVHPEPDHL"
     gene            complement(1484..1588)
                     /locus_tag="NBI57_002211"
     CDS             complement(1484..1588)
                     /locus_tag="NBI57_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1823140.1"
                     /translation="MLTNRLTSVNYVKKRLIQNIQRILSKEVLYGEMQ"
     gene            1683..2993
                     /gene="sbi"
                     /locus_tag="NBI57_002212"
     CDS             1683..2993
                     /gene="sbi"
                     /locus_tag="NBI57_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunoglobulin-binding protein Sbi"
                     /protein_id="HCD1823141.1"
                     /translation="MKNKYISKLLVGAATITLATMISNGEAKASENTQQTSTKHQTTQ
                     NNYVTDQQKAFYQVLHLKGITEEQRNQYIKTLREHPERAQEVFSESLKDSKNPDRRVA
                     QQNAFYNVLKNDNLTEQEKNNYIAQIKENPDRSQQVWVESVQSSKAKERQNIENADKA
                     IKDFQDNKAPHDKSAAYEANSKLPKDLRDKNNRFVEKVSIEKAIVRHDERVKSANDAI
                     SKLNEKDSIENRRLAQREVNKAPMDVKEHLQKQLDALVAQKDAEKKVAPKVEAPQIQS
                     PQIEKPKAESPKVEVPQIQSPKVEVPQSKLLGYYQSLKDSFNYGYKYLTDTYKSYKEK
                     YDTAKYYYNTYYKYKGAIDQTVLTVLGSGSKSYIQPLKVDDKNGYLAKSYAQVRNYVT
                     ESINTGKVLYTFYQNPTLVKTAIKAQETASSIKNTLSNLLSFWK"
     gene            3529..4458
                     /gene="hlgA"
                     /locus_tag="NBI57_002213"
     CDS             3529..4458
                     /gene="hlgA"
                     /locus_tag="NBI57_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bi-component gamma-hemolysin HlgAB subunit A"
                     /protein_id="HCD1823142.1"
                     /translation="MIKNKILTATLAVGLIAPLANPFIEISKAENKIEDIGQGAEIIK
                     RTQDITSKRLAITQNIQFDFVKDKKYNKDALVVKMQGFISSRTTYSDLKKYPYIKRMI
                     WPFQYNISLKTKDSNVDLINYLPKNKIDSADVSQKLGYNIGGNFQSAPSIGGSGSFNY
                     SKTISYNQKNYVTEVESQNSKGVKWGVKANSFVTPNGQVSAYDQYLFAQDPTGPAARD
                     YFVPDNQLPPLIQSGFNPSFITTLSHERGKGDKSEFEITYGRNMDATYAYVTRHRLAV
                     DRKHDAFKNRNVTVKYEVNWKTHEVKIKSITPK"
BASE COUNT         1798 a          818 c          643 g         1392 t
ORIGIN      
        1 taacaaatca tattatacac ctttgttcgc tatttttcta aggtttaaaa aatattttta
       61 ggtaagccta aaaatagatg taataaaaac gcctcctcag atatttatat atctatgaag
      121 acgtttaaat acattataga tggtctggtt ctgggtgaac gtatactgag gaaatacctt
      181 ttttgtgcaa atgatgttcg acattgtcac aaatttgatg cgcttctact aaggaaaggt
      241 tagcatctac aacaattgtg acatcaataa acacactact tccatggtaa cgccctttaa
      301 tacttttaac ttcttgtact tcatcaactt ctaaaatatc attgcgatac gcttctaatt
      361 cagtttcatt gaaaccatca ctcaacataa aaattgcttc tttaaaaata ccaaaaccag
      421 tataaacgat tagtaagcct agtaatgttg ctaaaataat atcgacaatt gggaaaccga
      481 tttgcgtaaa aattaatcct atcgctgttc caatgctgac taaactatct gataaattat
      541 ctttggcagc cgaatttaaa gaactacttt tcgttctttt cgctagtctt tgattgactg
      601 caaatacaat caacattaca agaccactga ttaagctgac gataattgtt attgcgttag
      661 gtacaacgtc atcttctttg aacaaacgag gtgcattttg aataactact tggataccta
      721 caaacataat gacaaatgac accaataatg aagaaatatt ttcagacttc aaatggccat
      781 aaggatgatt tcgatcggca ggtttaattg aaattttcaa tccaataata acagctaaag
      841 aaacgataat atctgtcata ttgtttaatg cgtcggctct tacagctgca gagttaaaga
      901 caaaacccgt gacatactta acaatagata agattatata tacaattaaa ctcaaataag
      961 caccgcgttg cgccaattta agattttcat tatgagacat gcgttgaacc accttgaatt
     1021 agtatagtaa caatattatg aatgattcat tttaatttta caacgttttt aatttttata
     1081 attttttata aaattaaact aatttattca ttcgcaaccc ctaaaaataa tttttagcct
     1141 ttcttcaaat ttatttgtta gaaaggcacc caactctccc tgtttgttaa ctttcgtctc
     1201 gaaagtttct atgttgggac ccaatgcatt agtagcgaag ctatctaatg cgttccttta
     1261 ttttctgcga attttatgag ctagaaagcc gcccaactct tcctgtttgt gaactttcgc
     1321 ctcgaaagtt tctatgttgg gaccctatgc attagttgcg aagctatcta atgtcgtgac
     1381 ctaattatat agaagaaaaa gtgcatcagt gacaaattaa atgagatttc tactctacca
     1441 aactctcttc gaaagacaat ttcctcctct atttattagc aaactattgc atttctccat
     1501 atagtacttc cttacttaaa atacgctgaa tgttctgaat taaacgcttt tttacatagt
     1561 taacactagt taatctatta gttaacatta gttaataatt agttaatttc catttgtatt
     1621 ctcatgtgat aaattctaaa agcatacaat aaatttaata tgtaaaaaga aagggaatac
     1681 acatgaaaaa taaatatatc tcgaagttgc tagttggggc agcaacaatt accttagcta
     1741 caatgatttc aaatggggaa gcaaaagcga gtgaaaacac gcaacaaact tcaactaagc
     1801 accaaacaac tcaaaacaac tacgtaacag atcaacaaaa agctttttat caagtattac
     1861 atctaaaagg tatcacagaa gaacaacgta accaatacat caaaacatta cgcgaacacc
     1921 cagaacgtgc acaagaagta ttctctgaat cacttaaaga cagcaagaac ccagaccgac
     1981 gtgttgcaca acaaaacgct ttttacaatg ttcttaaaaa tgataactta actgaacaag
     2041 aaaaaaataa ttacattgca caaattaaag aaaaccctga tagaagccaa caagtttggg
     2101 tagaatcagt acaatcttct aaagctaaag aacgtcaaaa tattgaaaat gcggataaag
     2161 caattaaaga tttccaagat aacaaagcac cacacgataa atcagcagca tatgaagcta
     2221 actcaaaatt acctaaagat ttacgcgata aaaataaccg ctttgtagaa aaagtttcaa
     2281 ttgaaaaagc aatcgttcgt catgatgagc gtgtgaaatc agcaaatgat gcaatctcaa
     2341 aattaaatga aaaagattca attgaaaaca gacgtttagc acaacgtgaa gttaacaaag
     2401 cacctatgga tgtaaaagag catttacaga aacaattaga cgcattagta gctcaaaaag
     2461 atgctgaaaa gaaagtggcg ccaaaagttg aggctcctca aattcaatca ccacaaattg
     2521 aaaaacctaa agcagaatca ccaaaagttg aagtccctca aatccaatca ccaaaagttg
     2581 aggttcctca atctaaatta ttaggttact accaatcatt aaaagattca tttaactatg
     2641 gttacaagta tttaacagat acttataaaa gctataaaga aaaatatgat acagcaaagt
     2701 actactataa tacgtactat aaatacaaag gtgcgattga tcaaacagta ttaacagtac
     2761 taggtagtgg ttctaaatct tacatccaac cattgaaagt tgatgataaa aacggctact
     2821 tagctaaatc atatgcacaa gtaagaaact atgtaactga gtcaatcaat actggtaaag
     2881 tattatatac tttctaccaa aacccaacat tagtaaaaac agctattaaa gctcaagaaa
     2941 ctgcatcatc aatcaaaaat acattaagta atttattatc attctggaaa taatcaatca
     3001 aaaatatctt ctctagtttt acatcatttt ttaaataatt ttcgtaataa accgtgatta
     3061 aaaagaaccg ctgattctca atcgaatctg tggttctttt tttattttcc atcaattaaa
     3121 tgtttcttcg ctatttgtca gtccacttct ttacctgcaa cttgttaaat aatccttaca
     3181 tcgttaacga atagttcatc atttagttga atcagctcaa ctttattaac ttcaaatttt
     3241 cacaaactat tgcgaaatcc attcctcttc cactacaagc accataatta aacaacaatt
     3301 caatagaata agacttgcaa aacatagtta tgtcgctata taaacgcctg cgaccaataa
     3361 atcttttaaa cataacataa tgcaaaaaca tcatttaaca atgctaaaaa tgtctcttca
     3421 atacatgttg atagtaatta acttttaacg aacagttaat tcgaaaacgc ttacaaatgg
     3481 attattatat atatgaactt aaaattaaat agaaagaaag tgatttctat gattaaaaat
     3541 aaaatattaa cagcaacttt agcagttggt ttaatagccc ctttagccaa tccatttata
     3601 gaaatttcta aagcagaaaa taagatagaa gatatcggtc aaggtgcaga aatcatcaaa
     3661 agaacacaag acattactag caaacgatta gctataactc aaaacattca atttgatttt
     3721 gtaaaagata aaaaatataa caaagatgcc ctagttgtta agatgcaagg cttcatcagc
     3781 tctagaacaa catattcaga cttaaaaaaa tatccatata ttaaaagaat gatatggcca
     3841 tttcaatata atatcagttt gaaaacgaaa gactctaatg ttgatttaat caattatctt
     3901 cctaaaaata aaattgattc agcagatgtt agtcagaaat taggctataa tatcggcgga
     3961 aacttccaat cagcgccatc aatcggaggc agtggctcat tcaactactc taaaacaatt
     4021 agttataatc aaaaaaacta tgttactgaa gtagaaagtc agaactctaa aggtgttaaa
     4081 tggggagtga aagcaaattc atttgttaca ccgaatggtc aagtatctgc atatgatcaa
     4141 tacttatttg cacaagaccc aactggtcca gcagcaagag actatttcgt cccagataat
     4201 caattacctc ctttaattca aagtggcttt aatccatcat ttattacaac attgtcacac
     4261 gaaagaggta aaggtgataa aagcgagttt gaaatcactt acggcagaaa catggatgct
     4321 acatatgctt acgtgacaag acatcgttta gccgttgata gaaaacatga tgcttttaaa
     4381 aaccgaaacg ttacagttaa atatgaagtg aactggaaaa cacatgaagt aaaaattaaa
     4441 agcatcacac ctaagtaaac agttcaatca ttttaaaaaa tcctgggaca cttcatactt
     4501 gtctcaggat tttttaacat attgaatcag cctcataaca ttaaattaat ttatcgtaca
     4561 ttaaatttaa taataacaac tgatttttat aagaacaaag tatcgcacca gagtagatac
     4621 acaaataata caaatgaatc catttaagtt g
//
LOCUS       DAHHSC010000183         4618 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.184, whole genome shotgun sequence.
ACCESSION   DAHHSC010000183 DAHHSC010000000
VERSION     DAHHSC010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 4618)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4618)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4618
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.184"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            complement(115..1833)
                     /gene="ptsP"
                     /locus_tag="NBI57_002214"
     CDS             complement(115..1833)
                     /gene="ptsP"
                     /locus_tag="NBI57_002214"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HCD1823143.1"
                     /translation="MSKLIKGIAASDGVAIAKAYLLVEPDLTFDKNEKVTDVEGEVAK
                     FNSAIEASKVELTKIRNNAEVQLGADKAAIFDAHLLVLDDPELIQPIQDKIKNENANA
                     ATALTDVTTQFVTIFESMDNEYMKERAADIRDVSKRVLSHILGVELPNPSMIDESVVI
                     VGNDLTPSDTAQLNKEFVQGFATNIGGRTSHSAIMSRSLEIPAIVGTKSITQEVKQGD
                     MIIVDGLNGDVIVNPTEDELIAYQDKRECYFADKKELQKLRDADTVTVDGVHAELAAN
                     IGTPNDLPGVIENGAQGIGLYRTEFLYMGRDQMPTEEEQFEAYKEVLEAMDGKRVVVR
                     TLDIGGDKELSYLNLPEEMNPFLGYRAIRLCLAQQDIFRPQLRALLRASVYGKLNIMF
                     PMVATINEFREAKAILLEEKENLKNEGHDISDDIELGIMVEIPATAALADVFAKEVDF
                     FSIGTNDLIQYTLAADRMSERVSYLYQPYNPSILRLVKQVIEASHKEGKWTGMCGEMA
                     GDETAIPLLLGLGLDEFSMSATSILKARRQINGLSKNEMTELANRAVDCATQEEVIEL
                     VNNYVK"
     gene            complement(1836..2102)
                     /locus_tag="NBI57_002215"
     CDS             complement(1836..2102)
                     /locus_tag="NBI57_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HCD1823144.1"
                     /translation="MEQNSYVIIDETGIHARPATMLVQTASKFDSDIQLEYNGKKVNL
                     KSIMGVMSLGVGKDAEITIYADGSDESDAIQAISDVLSKEGLTK"
     gene            complement(2256..2798)
                     /locus_tag="NBI57_002216"
     CDS             complement(2256..2798)
                     /locus_tag="NBI57_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF697 domain-containing protein"
                     /protein_id="HCD1823145.1"
                     /translation="MGFKNNLTSNLTNKIGNSVFKIENVDGKGAMPTTIQELRERRQR
                     AEAIVKRKSLMSSTMSVVPIPGLDFGVDLKLMKDIIEDVNKIYGLDHKQVNSLGDDVK
                     ERIMSAAAIQGSQFIGKRISSAFLKIVIRDVAKRTAAKQTKWFPVVGQAVSASISYYF
                     MNKIGKDHIQKCENVIKNVM"
     gene            complement(2852..4024)
                     /locus_tag="NBI57_002217"
     CDS             complement(2852..4024)
                     /locus_tag="NBI57_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HCD1823146.1"
                     /translation="MKIATLNKGKETKYFNGYPLIEEEDIYSQDHLKEGDIFQIVTDK
                     SQYVATAYVGRQHKGLGWVLTYDKAQEINTAFFVKLFNTALAERDYYFNIDGTNAFRL
                     FNAEGDGVGGLTIDNYDGHLLIQWYSKGIYKFKYAILEAVRKVFDYKSIYEKVRFKDS
                     EYCGGFVEGDAPEFPIVIEENFTFYNVDLEDGLMTGIFLDQKEVRKKLRGQYAKERHV
                     LNLFSYTGAFSVIAASEASSTTSVDLANRSRSLTEENFGLNAIDPKSQYIYVMDTFDF
                     YKYAARHGHSYDTIVIDPPSFARNKKRTFSVQKDYDKLINGALNILSSEGTLLLCTNA
                     SVYPLKQFKNTIKKTLEESGVDYELTEVMGLPKDFKTHPHYKPSKYLKAVFVNIRH"
BASE COUNT         1465 a          866 c          606 g         1681 t
ORIGIN      
        1 caacaacatt ccattgaaat taacataaat cataaaaaag aacccgaagt attcataatt
       61 actcacaatg aatacttcgg gtttatttat aattctgtct ttcgaaagaa ttaattattt
      121 tacgtagttg ttaactaatt caataacttc ttcttgcgtt gcacagtcga ctgcacggtt
      181 agcaagttca gtcatttcat ttttacttaa accattaatt tgtcttcttg ctttcagaat
      241 agacgttgca ctcatagaga actcatctaa acctaaacca agcaataatg gaatagctgt
      301 ttcatctcca gccatttcac cacacatacc tgtccattta ccttctttat gtgacgcttc
      361 aataacttgt ttaactaaac gtaagattga agggttatat ggttggtata gatatgatac
      421 acgctctgac atacggtcag cagctaatgt gtattgaatt aaatcatttg taccgatact
      481 gaagaaatct acttctttag caaagacatc agctaatgct gctgttgcag gtatctctac
      541 catgattcct aattctatat catccgaaat gtcatgacct tcatttttaa ggttttcttt
      601 ttcttctaat aatatagctt ttgcttctct aaattcgtta attgttgcaa ccattgggaa
      661 catgatattt aacttaccat aaactgatgc acgtaataat gcacgtagct gtggtctgaa
      721 aatatcttgt tgcgcaaggc ataaacgaat cgcacggtaa cctaagaatg gattcatttc
      781 ttcaggcaag tttaagtatg ataattcttt atctccacct atatctaaag tacgtacaac
      841 aacacgttta ccgtccattg cttctaatac ttctttataa gcttcaaatt gttcttcttc
      901 tgtaggcatt tggtcacgac ccatatataa aaactcagtt ctatataagc cgataccttg
      961 tgcaccattt tcaataacac ctggcaaatc attaggtgta ccaatatttg cagcaagctc
     1021 tgcgtgaaca ccatcaactg taacagtatc agcatcacgt agtttttgta attctttctt
     1081 gtcagcaaaa taacactcac gtttatcttg ataagcgatt aactcatctt cagttggatt
     1141 aacgattaca tcaccattta atccatctac gataatcatg tcgccttgtt taacttcttg
     1201 agtaattgat tttgtaccaa caattgctgg aatttctaaa gaacgactca taattgcaga
     1261 gtgacttgtt cttccgccaa tgtttgtagc aaaaccttgt acgaattctt tatttaattg
     1321 agcagtatca gatggcgtta agtcattccc tacaataaca acgctttcat caatcatact
     1381 cggattcggt aattctacac ctaaaatatg tgataacaca cgtttagaaa cgtcgcgaat
     1441 atcagccgca cgttctttca tgtattcgtt atccatagat tcaaaaattg taacaaattg
     1501 tgttgttaca tccgttaatg ctgtagcagc attagcgttt tcatttttaa tcttatcttg
     1561 gattggttga attaattcag ggtcatctaa aactaataaa tgtgcatcaa agatagcagc
     1621 tttatcagca cctagttgaa cctctgcatt atttctaatt ttagttaact caactttaga
     1681 agcttcgata gcgctattga actttgcaac ttctccttca acatcagtga ctttttcatt
     1741 tttgtcgaat gttaagtctg gctcaactaa taaataagct ttagcaattg cgacaccatc
     1801 agatgcagca atacctttaa ttaatttaga catgattatt tagtcaatcc ttcttttgat
     1861 aagacgtcac tgattgcttg aatggcgtca gattcatcac taccgtcagc ataaattgta
     1921 atttcagcat ctttaccaac accaaggctc ataacaccca tgattgattt taagtttact
     1981 ttcttaccgt tatattctaa ttgaatatca gaatcgaatt ttgaagctgt ttgtactaac
     2041 attgttgctg gtctagcgtg aataccagtc tcgtcgatga ttacatatga attttgttcc
     2101 ataattacat ttctccttcg tatagttaga atcttcaata ttattacaat actaacttag
     2161 ttttatacat taactttacc aaaaccatgc tttaaattca attcgtttaa aaccggtttc
     2221 agtaaaattt gcaaattgca aaactattat agcacctaca tgacattttt aataacattt
     2281 tcgcattttt gaatgtgatc ttttccaatt ttattcataa aatagtaact aatagatgca
     2341 gacacagctt gtcctacaac aggaaaccat tttgtttgtt ttgcagcagt acgtttagct
     2401 acatctctaa taactatttt taaaaatgca cttgaaattc ttttaccaat aaattgacta
     2461 ccttgaattg ctgctgcaga cataattctt tctttcacat catccccaag gctattaact
     2521 tgcttatgat ctaatccgta tattttattt acatcttcga taatatcttt cattaatttt
     2581 aaatcaacac caaaatctaa acccggaatt ggaacaacgc tcattgttga tgacattaaa
     2641 gactttctct ttacaattgc ttcagcacgt tgtcgtcttt ctctcaattc ttgaatcgtc
     2701 gttggcattg caccttttcc gtcaacattt tctattttaa agactgaatt accaatttta
     2761 tttgttaaat ttgatgttaa attgtttttg aatcccataa tattatcacc cttctttttc
     2821 tcaataagtt actatacccc tttttatcaa attaatgtct aatatttaca aaaacagctt
     2881 ttaaatattt agatggctta taatgtggat gcgttttaaa atcttttggt aaacccataa
     2941 cttcagttaa ttcataatca acgccactct cttcaagcgt ctttttaata gtatttttaa
     3001 attgctttaa tggatataca cttgcatttg tacataacaa taatgttcct tcagatgata
     3061 aaatatttaa ggcgccatta attaatttgt cataatcttt ttgcactgaa aatgtacgtt
     3121 ttttgttgcg tgcaaagcta ggtggatcaa tcacgatcgt gtcataacta tgtccatgtc
     3181 gtgcagcata tttatagaaa tcaaaagtgt ccatgacata aatatattgg gatttaggat
     3241 caatagcatt taatccaaaa ttttcttcag ttaaactacg agaacgatta gccaaatcta
     3301 cacttgttgt tgaagatgcc tcacttgctg ctattacaga aaaagcacct gtataactaa
     3361 ataagtttaa aacatggcgt tctttggcat attgacccct taatttcttg cgcacttctt
     3421 tttgatctaa aaagatacct gtcatcaaac catcttcaag gtctacatta taaaatgtga
     3481 agttttcttc gataacaatt ggaaactcag gtgcatcgcc ttcaacaaaa ccaccacaat
     3541 attcgctgtc tttaaatctt actttttcgt aaatagattt ataatcaaat acttttctaa
     3601 ccgcttcaag aatggcatat ttaaatttat aaataccttt tgagtaccat tgaatcaaca
     3661 aatgaccatc gtaattgtcg attgttaatc ccccaacacc atcaccttca gcattaaata
     3721 atctaaaagc atttgttcca tctatattaa aataataatc acgttctgct aatgcagtat
     3781 taaacaattt cacaaagaaa gctgtgttga tttcttgagc tttatcgtat gttagaaccc
     3841 aacctaatcc tttatgttgg cgaccaacat aagccgttgc aacatattgt gatttatcag
     3901 tcacaatttg aaaaatatct ccttctttta aatgatcttg tgaatagata tcctcttctt
     3961 caattaaagg atatccatta aaatattttg tttctttgcc tttgttcaga gttgctattt
     4021 tcatctgaca acttcctttc tgtgtttcgt tgtagtttta tatagattaa attgtttgcc
     4081 aaatttcaat atcatctact tctaatctta ctgttgattt taacaaatta gtaattcgaa
     4141 tgatatatgt taaatttcta caaaggaaat actatatatc gtttcgcaaa ttccatatgt
     4201 cgcttcaact atttactagt tttcttgatt ttacaaatgg ataacttttc aactatttta
     4261 tttgcactaa aactttgtat acaattttca atacttaatt cacatcacct gaatcaatca
     4321 aatctagtta caaaaaaagt actatccata aacttcattg acctgtatct aaataagtta
     4381 gagatatatc taacttatag cgtacacatc aaaatgttta atagatagta cttttataat
     4441 gcttttaatt atggtcgctc tcgtaattgt aacgaataag aaatgatgat tgccaataac
     4501 attaatagta atgttggcgt aatgaacact gaaagtagta gtccatgaat cattatattt
     4561 ataaattccg ttaacaattt aaagaataaa atgctcccca taaatagttg taaataat
//
LOCUS       DAHHSC010000184         4573 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.185, whole genome shotgun sequence.
ACCESSION   DAHHSC010000184 DAHHSC010000000
VERSION     DAHHSC010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 4573)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4573)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4573
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.185"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            95..556
                     /gene="tsaE"
                     /locus_tag="NBI57_002218"
     CDS             95..556
                     /gene="tsaE"
                     /locus_tag="NBI57_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000590797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HCD1823147.1"
                     /translation="MIKINNLDEMNQFAMFLVEQLKSGDLILLNGDLGAGKTTLTQFI
                     GKALGVRRTINSPTFNIIKSYRGKNLKLHHMDCYRLEDSDEDLGFDEFFEDQAITVIE
                     WSQFIKDLLPATHLSINISTISENTRQIELFAQGEHYEQIKEAIIHEFAAH"
     gene            537..1199
                     /gene="tsaB"
                     /locus_tag="NBI57_002219"
     CDS             537..1199
                     /gene="tsaB"
                     /locus_tag="NBI57_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HCD1823148.1"
                     /translation="MNSLLIDTSNQPLSVALMQNDEVLAEITTDSKQNHSVQLMPAIS
                     QLFEQSKIAKQQLDAIIVAEGPGSYTGLRIGVTVAKTLAYALDVKLYGVSSLKALAAT
                     IDHTDKLLVPVFDARRQAVYTGIFQWQNGQLVTILEDQYMSIEALRAFLEDLNQPFIY
                     IGKDTIKLQDELQGEVIAQLPNASVMYQLIDKPTDVHTFTPKYHKLAEAERNWINSQK
                     NN"
     gene            1172..1636
                     /gene="rimI"
                     /locus_tag="NBI57_002220"
     CDS             1172..1636
                     /gene="rimI"
                     /locus_tag="NBI57_002220"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001611465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HCD1823149.1"
                     /translation="MDQQSKEQLNIREMTKEDVPQVFDLERRSFNDSSWTIDAFYHEI
                     EQNNFAKYFVLEFEQQIIGYLGLWIVIDQAQITTVAIDDNYRGYGLGQMLLKYGINYA
                     SHTCDVMSLEVRVNNKVAQHVYENLGFQYGGKRKNYYGEGEDAMVMWVNLND"
     gene            1629..2654
                     /gene="tsaD"
                     /locus_tag="NBI57_002221"
     CDS             1629..2654
                     /gene="tsaD"
                     /locus_tag="NBI57_002221"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HCD1823150.1"
                     /translation="MTKDILILAVETSCDETSVSVIKNGRDILSNTVLSQIESHKRFG
                     GVVPEVASRHHVEGITTTINEALVDADVSMEDIDAIAVTEGPGLIGALLIGVNAAKAL
                     AFAYDKPLIPVHHIAGHIYANHIEEPLTFPLIALIVSGGHTELVYMKDHLSFEVIGET
                     RDDAVGEAYDKVARTIGLNYPGGPQVDRLAAEGEDTYSFPRVWLDKDSYDFSFSGLKS
                     AVINQLHNQRQKNIPIIEANVATSFQNSVVEVLTFKAIQACKEYSVQRLIVAGGVASN
                     KGLRQSLADQCKVNDIQLTIPSPKLCTDNAAMIGVAGHYLYQQGRFADLALNGHSNID
                     LEEYSAE"
     gene            complement(2969..>4573)
                     /locus_tag="NBI57_002222"
     CDS             complement(2969..>4573)
                     /locus_tag="NBI57_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MutS family DNA mismatch repair protein"
                     /protein_id="HCD1823151.1"
                     /translation="TNQTFLIFVIAIILLTSVIGIVGRYMSRQRLLKSMETLWQTISP
                     LEAFIRPNSHFDYEYKLYKEKFESHSLVDDKTWSDLNMNAIFHKMNYNLTAIGEMKLY
                     ACLRGMLSITNKSLLSLFNDNAEFRKNVTYHLALIGKTVYPTFPDQITPVKRQNILFL
                     CPFLPVISFAVIFINSQVGILLFLMSCLFNIILSATLKRTYEDDLKSIFYASNVLKQG
                     YTISKIKHAPQPEVNFKQFRTARHLTSVLAEVNDEDIGAMVIKLVKLIFMLDYVLFHS
                     IQKSYTTHMNELKNCFDYIAELDNHYALAMYRRTLECYTEPQIDDTNDGIVFSELTHP
                     LIADAVANDFSLSQNILLTGSNASGKSTFMKSIAINIILASAIQTVTASKFVYQPGIV
                     FTSMANADDVLSGDSYFMAELKSIKRIVEIPDNQKIYCFIDEIFKGTNTTERIAASES
                     VLSFLHEKSNFRVIAATHDIELAELLKQRYENYHFNEVIENNNIHFDYKIKPGKANTR
                     NAIELLKITSFPAKIYERAKDNVSNG"
BASE COUNT         1564 a          655 c          857 g         1497 t
ORIGIN      
        1 tgttacaata taaaggttaa atataaagtt agatacgatt gaacaatgga taatcatttt
       61 tatgaatgaa aagcataata taggagaatc aacattgata aagataaata atttagatga
      121 aatgaatcaa tttgctatgt ttttagttga gcaattgaaa agtggtgatt tgattttact
      181 taacggagat ttaggagcag gtaaaacaac gttaacgcaa tttataggaa aagctcttgg
      241 tgtaagacgt acgattaatt ccccgacatt taacatcatt aaatcatata ggggtaaaaa
      301 tttaaaattg catcatatgg attgttatcg cttagaagat tctgatgaag atttagggtt
      361 tgatgaattt ttcgaagatc aggcaattac tgttattgaa tggagtcaat ttataaaaga
      421 tttacttcca gcgacgcatt tatctattaa catttcaaca atatctgaaa atacaagaca
      481 aattgagttg ttcgcgcaag gagaacatta tgaacaaatt aaggaggcaa ttatccatga
      541 attcgctgct cattgataca tcgaatcaac cattatcagt agcattgatg caaaacgatg
      601 aagttttagc tgaaataact acggattcta aacaaaatca ttcagtgcaa ttaatgcctg
      661 cgattagtca gctatttgag cagagcaaaa tagctaaaca gcaattagat gcgattattg
      721 ttgctgaagg tcctggttca tatactggct tacgtatcgg tgttactgtt gctaaaacat
      781 tagcgtatgc gttagatgtt aaattatatg gtgtttcatc attaaaggca ttggcagcaa
      841 caatcgatca tactgataaa ttattggttc ctgtgtttga tgcaagacga caagcagttt
      901 atacaggcat attccagtgg caaaatggtc aattagtgac tattttagaa gatcaatata
      961 tgtcgataga ggcattgcga gcttttttag aagatttaaa ccaaccattt atttatattg
     1021 ggaaagatac tataaaatta caagatgaat tacaaggtga agtgattgcg cagctaccaa
     1081 atgcatcagt catgtatcaa ttgatagata aacctacaga cgtacataca tttactccta
     1141 aataccataa attagctgag gcggaacgaa attggatcaa cagtcaaaag aacaattaaa
     1201 cattagagag atgactaaag aagatgtgcc acaagtcttt gatcttgagc gtcggagttt
     1261 caatgacagt tcttggacaa ttgacgcatt ttatcatgaa atagagcaaa ataactttgc
     1321 gaaatatttt gtcttagaat ttgaacaaca aattattggt tatttaggtt tatggattgt
     1381 tatcgatcaa gctcaaatta caacagttgc aatcgatgat aattatagag gctatggttt
     1441 aggtcaaatg cttttaaaat acggaataaa ctatgcaagt cacacttgtg atgtgatgag
     1501 tttagaagta agagttaata ataaagtggc acaacatgta tatgaaaatt taggatttca
     1561 atatggtggt aaacgtaaaa attattatgg tgaaggtgag gacgcaatgg ttatgtgggt
     1621 gaatttaaat gactaaagat atattaatac tagctgttga aacaagttgt gatgaaacaa
     1681 gcgttagtgt tataaaaaat ggcagagata ttttatcaaa tacagtttta agtcagattg
     1741 aaagtcataa acgatttggc ggtgtcgttc ccgaagtggc aagtagacat cacgttgaag
     1801 gtataacaac aacaataaac gaggctctag tggatgccga tgtatcaatg gaagatattg
     1861 atgccatagc ggttacagaa ggccctggac taattggtgc gttactaata ggtgttaatg
     1921 cagccaaagc attggcattt gcttacgata agccacttat tcctgttcat catattgcag
     1981 gacatatata tgctaatcac atagaagagc cattaacatt cccgctaatt gcacttattg
     2041 tttcaggtgg acatactgaa ttagtttata tgaaagatca tttatcattt gaagtcattg
     2101 gtgaaacacg agatgacgca gtaggtgagg cttatgataa agtggcacga acaattggtt
     2161 taaattatcc aggtggtcca caagttgatc ggttggctgc tgaaggtgaa gatacttatt
     2221 cattccctcg tgtttggttg gataaagata gttatgattt tagttttagt gggttgaaaa
     2281 gtgccgtgat caatcaactt cacaatcaac gacaaaaaaa tattccaatc attgaagcta
     2341 acgtagcaac gagctttcaa aatagtgttg tagaggtgct tacgtttaaa gctattcaag
     2401 cttgtaaaga atatagtgtt cagcgattaa ttgttgctgg tggcgtggcg agtaataaag
     2461 gattacgtca atctttagcg gatcaatgca aagtcaatga cattcaatta actatcccaa
     2521 gtcctaaatt atgcacagat aatgctgcaa tgataggcgt tgccggccac tatttgtatc
     2581 agcaaggtcg atttgctgat ttagcattaa atgggcacag caatatagat ttagaagagt
     2641 attctgcaga ataaaataaa cgtcagttat taacatgatt acgatgaaca ttattgaaac
     2701 tgttcatagt cttctgtcat gtgatagctg acgtttttat attgcaatta ggggagtgag
     2761 acagaaaaga taaagaacca ctaatgattt attatgttgg gattctgcca acttgcacat
     2821 tattgtaagc tgactttccg ccagcctctg tattggggcc cccggactag tattgaaaaa
     2881 acttattaca agcgtatttt catttagtca aagactgtca atataacaat gttgcgactt
     2941 gaacattgat tgatgttcaa gattcatgct aaccattgga aacattatct tttgctcgtt
     3001 catatatttt tgctggaaat gaagtgattt ttaataattc gatggcatta cgagtatttg
     3061 ctttgccagg cttaattttg taatcaaaat gtatgttatt attttctatt acctcattga
     3121 aatgataatt ttcataacgt tgttttaaga gttcagctaa ctcaatatca tgtgttgctg
     3181 caataactct aaagttagat ttttcatgta aaaatgatag tactgattct gaagcggcaa
     3241 ttcgttcagt tgtgttggta cctttaaaaa tttcatcgat aaagcagtat attttttgat
     3301 tatctggaat ttcaacaata cgcttaatag attttaattc agccataaaa taactatcgc
     3361 ctgataacac atcatcggca tttgccattg acgtgaacac aattccaggt tgatatacaa
     3421 atttgcttgc ggtaactgtt tgtatagccg atgcaagaat aatgtttatt gctatggatt
     3481 tcataaatgt tgatttaccc gaagcattag acccagttaa cagtatattt tgtgaaagtg
     3541 agaaatcatt ggctactgca tctgctatga gtggatgtgt taactcagag aatactatgc
     3601 catcatttgt atcatcaatt tgtggttcag tataacattc tagcgttcta cgatacattg
     3661 ctaatgcata atgattatct aattcagcta tgtaatcgaa acaattttta agctcattca
     3721 tatgtgttgt atagcttttt tgaattgaat gaaataaaac ataatcaagc atgaaaataa
     3781 gtttaactag tttaattacc atagctccga tatcttcatc attaacttca gctaaaacac
     3841 tcgttaaatg acgtgctgtt ctaaattgct taaaattgac ttcaggttgt ggagcatgtt
     3901 taatttttga gatagtatag ccttgtttta aaacattgga tgcataaaag atagatttta
     3961 agtcatcctc atatgtacgc tttaaagttg ctgataaaat gatattaaat aggcaactca
     4021 taagaaataa taaaatacca acttgtgaat taataaatat aactgcaaat gagataactg
     4081 gtaaaaacgg gcatagaaac aatatatttt gacgttttac cggtgtgatt tggtctggaa
     4141 atgttggata aacagtttta ccaatcaaag ctaaatgata tgttacgttt tttctaaatt
     4201 cagcattatc attaaataaa ctaagtaatg atttgttcgt aattgaaagc attccacgta
     4261 aacaggcata tagcttcatt tcaccaatag ctgttaaatt ataattcatc ttatgaaaaa
     4321 ttgcattcat atttaagtcg gaccaagttt tatcatcaac taatgaatgt gattcaaatt
     4381 tttccttgta gagcttatac tcatagtcga aatgtgagtt cggtctgata aaagcttcta
     4441 atggagaaat cgtttgccat aatgtttcca tagattttaa tagacgttga cgactcatgt
     4501 atcgtccaac aattcctatt acagatgtaa gtagaataat tgctataaca aatattaaaa
     4561 atgtttgatt tgt
//
LOCUS       DAHHSC010000185         4557 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.186, whole genome shotgun sequence.
ACCESSION   DAHHSC010000185 DAHHSC010000000
VERSION     DAHHSC010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 4557)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4557)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4557
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.186"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            212..1255
                     /locus_tag="NBI57_002223"
     CDS             212..1255
                     /locus_tag="NBI57_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCD1823152.1"
                     /translation="MNKDNKWTMITALFITVISVLLAFHLKQHYDQITNENHANKDKI
                     NIKNKNVRIYQNLTYNRVFPNSKLDIITPVDMSSNAKLPVIFWMHGGGYIAGDKQYKN
                     PLLAKIAEQGYIVVNVNYALAPQYKYPTPLIQMNQATQFIKENKMNLPIDFNQVIIGG
                     DSAGAQLASQFTAIQTNDRLREAMKFDQSFKPSQIKGAILFGGFYNMQTVRETEFPRI
                     QLFMKSYTGEEDWEKSFKNISQMSTVKQSTKNYPPTFLSVGDSDPFESQNIEFSKKLQ
                     ELNVPVDTLFYDGTHHLHHQYQFHLNKPESIDNIKKVLLFLSRNTSSSGIQTEEKPQM
                     ENPSNELPLNPLN"
     gene            1584..2012
                     /locus_tag="NBI57_002224"
     CDS             1584..2012
                     /locus_tag="NBI57_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1823153.1"
                     /translation="MTRSALKPFKNKRVMVTGRIQRVLFKNYLDRHSTFKPNVRILLK
                     DVFVSGVSIDHLWLYETNKYYALAMELIHQRVKFSANVVPYYKINRNNNLFVQDYGIK
                     RKGRLITEEAYNQNNQYQDKIYEKLPDIDFRLEDFYSKEN"
     gene            complement(2221..2727)
                     /locus_tag="NBI57_002225"
     CDS             complement(2221..2727)
                     /locus_tag="NBI57_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCD1823154.1"
                     /translation="MAHIIRRVSIKDVENFISMLAKIYDESPYMFYTPGEYDPSVTSA
                     SKQLEEYITSPHKVIFVAESDEQLVGFAFVNTTPFQRIKHVAKIDLGVKKLYQHRGIG
                     QALLDAIMAWCLNNQIHRIEANVPLNNQPALELFKSADFQIEGVLKDKLFIDGKYYDD
                     YMMAKILN"
     gene            2840..3763
                     /gene="graX"
                     /locus_tag="NBI57_002226"
                     /gene_synonym="apsX"
     CDS             2840..3763
                     /gene="graX"
                     /locus_tag="NBI57_002226"
                     /gene_synonym="apsX"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="auxiliary protein GraX/ApsX"
                     /protein_id="HCD1823155.1"
                     /translation="MKPKVLLAGGTGYIGKYLSEVIENDAELFTISKYPDNKKTDDVE
                     MTWIQCDIFHYEQVVAAMNQIDIAVFFIDPTKNSAKITQSSARDLTLIAADNFGRAAA
                     INQVKKVIYIPGSRYDNETIERLGAYGTPVETTNLVFKRSLVNVELQVSKYDDVRSTM
                     KVVLPKGWTLKNVVNHFIAWMGYTKGTFVKTEKSHDQFKIYIKNKVRPLAVFKIEETA
                     DGIITLILLSGSLVKKYTVNQGKLEFRLIKESAVVYIHLYDYIPRLFWPIYYFIQAPM
                     QKMMIHGFEVDCRIKDFQSRLKSGENMKYTK"
     gene            3779..4453
                     /gene="graR"
                     /locus_tag="NBI57_002227"
                     /gene_synonym="apsR"
     CDS             3779..4453
                     /gene="graR"
                     /locus_tag="NBI57_002227"
                     /gene_synonym="apsR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor
                     GraR/ApsR"
                     /protein_id="HCD1823156.1"
                     /translation="MQILLVEDDNTLFQELKKELEQWDFNVAGIEDFGKVMDTFESFN
                     PEIVILDVQLPKYDGFYWCRKMREVSNVPILFLSSRDNPMDQVMSMELGADDYMQKPF
                     YTNVLIAKLQAIYRRVYEFTAEEKRTLTWQDAVVDLSKDSIQKGDDTIFLSKTEMIIL
                     EILITKKNQIVSRDTIITALWDDEAFVSDNTLTVNVNRLRKKLSEISMDSAIETKVGK
                     GYMAHE"
     gene            4446..>4557
                     /locus_tag="NBI57_002228"
     CDS             4446..>4557
                     /locus_tag="NBI57_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HCD1823157.1"
                     /translation="MNNLKWVAYFLKSRMNWIFWILFLNLLMLGISLIDYD"
BASE COUNT         1702 a          598 c          786 g         1471 t
ORIGIN      
        1 agtagctgta attattaaat atttcaaacg taaaaatgcg gataaaaaac atattgctta
       61 atatcatatg atggatatcc gaaactttac aattgaatca ctttgtttta accttaaaag
      121 caattcgtct ctactcttat cgggcgaatt gctttttata tttattcagt ctattaatat
      181 gagcgtctaa caaatagaga ggtacgatgt aatgaataaa gataataaat ggacgatgat
      241 aactgcgctt tttataactg taatcagtgt attgttagca tttcatctga aacaacatta
      301 tgaccaaatt acaaatgaga accatgctaa taaagacaaa attaatatta aaaataaaaa
      361 tgtgcgcatt tatcaaaacc ttacatacaa tagagttttc cctaacagta aattagatat
      421 tattacacct gttgatatgt cttctaatgc caaactgcca gttatttttt ggatgcacgg
      481 tggtggttat attgcgggtg ataagcagta taaaaaccca ttattagcga aaattgctga
      541 acaagggtac attgttgtga atgtaaatta tgcattggcg ccacaatata aatatcccac
      601 accattaatt caaatgaatc aagcaactca attcattaaa gaaaataaaa tgaatttacc
      661 tattgatttt aatcaagtaa ttattggcgg tgattctgca ggtgctcaat tagctagcca
      721 atttacggca atacagacga atgatcgctt aagagaagcc atgaaatttg atcagtcatt
      781 caaaccatcg caaattaaag gtgctatact atttggtggt ttttataata tgcaaacagt
      841 tagagaaact gagtttccaa gaatacagtt atttatgaaa agttatactg gcgaagaaga
      901 ttgggaaaag agctttaaaa atatttcaca aatgtcgaca gtaaaacaat cgacaaaaaa
      961 ttatccacca acatttttat ctgttggaga tagcgatcca ttcgaaagtc aaaatataga
     1021 attcagtaag aaattacaag aattgaatgt accagtagat actttgtttt atgatggtac
     1081 gcatcattta catcatcagt atcaatttca ccttaataaa cctgaatcga tagataacat
     1141 caaaaaagtg ttacttttct taagtcgtaa tacatcttct agtggtattc aaactgaaga
     1201 gaaaccacaa atggaaaatc cgagtaatga attaccgtta aatcctttaa actaatgata
     1261 aacagtagta atttattact taagcaacat ttaagatctt caaattgaaa acgaagaatt
     1321 taaaacatgt ggggctaatg tgtaagagtc tgagatataa taaattgtta gcggttcttt
     1381 atcatttcta tctcacccta ttatgatgtg acatttattt tacaaactaa tttgttttga
     1441 acctgaaaat aactttttac aactaaattg tcgaaaaaca gtgtgtacat gatataataa
     1501 atttataaat tgaaaagaat tcaaaagaaa aaataaaata tagattgagc acgaagtgat
     1561 ttgaaataag gttgtgaaag ggaatgacaa ggtcagcatt aaaaccattt aaaaataaac
     1621 gcgttatggt tactggacgt atacaacgtg ttttgtttaa aaattattta gatagacata
     1681 gcacatttaa gccgaatgta aggatattat taaaagatgt atttgtttca ggtgtatcaa
     1741 tagatcattt atggttatat gagacaaata aatactatgc attggcaatg gaacttattc
     1801 atcaacgagt aaaatttagt gcgaatgttg taccatatta caaaataaat agaaataata
     1861 atttattcgt acaagattat ggaattaagc gtaaaggtag gttaattact gaagaagctt
     1921 acaatcaaaa caatcagtat caggataaga tatatgaaaa attaccggat atagatttta
     1981 gactcgaaga tttttatagt aaggaaaact aaacaacata tataaagcac tactaatcgt
     2041 agttgatagt tgatcaatta cacattagtg gtgctttatt ttgaatttaa atttgataaa
     2101 tgaataagag tatacgcatt catttaagat tagatgacat tgataagtat gagtaatgta
     2161 ttaggtaacc attaaattcg ttgttatgcg attctgatac aagattatga taaaatagct
     2221 ttaattaaga attttagcca tcatatagtc atcgtaatat ttaccatcga taaataactt
     2281 atcttttaaa acgccttcga tttgaaaatc ggcactttta aaaagctcga gggcaggttg
     2341 gttattgagt ggtacatttg cttcaattcg gtgtatttga ttgtttaaac accaagccat
     2401 aatggcatca agaagtgctt ggccaattcc acgatgttga tataatttct ttacacctaa
     2461 atcaatttta gcaacatgtt taatgcgttg aaatggtgtc gtattaacaa aggcaaagcc
     2521 aacgagttgt tcatcacttt cagcaacaaa gatgacttta tgtggagaag tgatatattc
     2581 ttctaattgt ttactagccg acgtgacgct aggatcatat tctcctggtg tgtagaacat
     2641 atacggagat tcgtcgtata ttttcgctaa cattgaaatg aaattttcta catctttgat
     2701 actaactcta cgtataatat gggccataaa aagcctccag tattttgaaa ttttaaaaca
     2761 tttgctacta ttataatata tatgtaacta aaaggtggag taatatgttt tttggttaca
     2821 gtttaaagga gtattttaga tgaaacctaa agttttatta gcaggtggaa caggatatat
     2881 tggtaagtat ttaagtgaag tgattgaaaa tgatgctgaa ctttttacta tatcaaaata
     2941 tccagacaat aaaaaaacag atgatgttga aatgacttgg attcagtgtg atatatttca
     3001 ttacgaacag gttgttgcag caatgaatca aatagatatt gctgtattct ttatcgaccc
     3061 aacaaagaat tctgccaaaa taacacaatc atcagcaaga gatttaacat taatcgcagc
     3121 agataatttt ggtcgagcag cggctatcaa tcaagtaaaa aaagtaatct acatacctgg
     3181 gagtcgttat gataatgaaa caattgaacg cctaggtgca tatggcacac ctgtagaaac
     3241 aacaaattta gtttttaaac gttctttagt taatgtagaa ttacaagttt caaagtatga
     3301 tgatgttaga tcaacgatga aggtagtttt accaaaggga tggacattaa agaacgttgt
     3361 aaaccatttt attgcatgga tgggttacac taaaggaact tttgtgaaaa cagaaaaatc
     3421 acatgatcaa tttaagatat atattaagaa taaggtgcga ccgctcgcag tatttaaaat
     3481 agaagaaaca gctgacggaa taataacttt aattttattg agtggaagtt tagtgaaaaa
     3541 atacacagtt aatcaaggga agttagaatt tagattaatc aaagagtcgg cagtcgttta
     3601 tatacatcta tacgattata tccctcgatt attttggccg atttattact ttatacaagc
     3661 accaatgcaa aaaatgatga ttcatggctt tgaagttgac tgccggatta aagattttca
     3721 aagtcgatta aaatcaggag aaaatatgaa atatactaaa tgatattggg tgatatggat
     3781 gcaaatacta ctagtagaag atgacaatac tttgtttcaa gaattgaaaa aagaattaga
     3841 acaatgggat tttaatgttg ctggtattga agatttcggc aaagtaatgg atacatttga
     3901 aagttttaat cctgaaattg ttatattgga tgttcaatta cctaaatatg atgggtttta
     3961 ttggtgcaga aaaatgagag aagtttccaa cgtaccaata ttatttttat catctcgtga
     4021 taatccaatg gatcaagtga tgagtatgga acttggcgca gatgattata tgcaaaaacc
     4081 tttctatacc aatgtattaa ttgctaaatt acaagcgatt tatcgtcgtg tctatgagtt
     4141 tacagctgaa gaaaaacgta cattgacttg gcaagatgct gtcgttgatc tatcaaaaga
     4201 tagtatacaa aaaggtgacg atacgatttt tctatccaaa acagaaatga ttatattaga
     4261 aattcttatt accaaaaaaa atcaaatcgt ttcgagagat acaattatca ctgcattatg
     4321 ggatgatgaa gcatttgtta gtgataatac gttaacagta aatgtgaatc gtttacgaaa
     4381 aaaattatct gaaattagta tggatagtgc aatcgaaaca aaagtaggaa aaggatatat
     4441 ggctcatgaa taatttgaaa tgggtagctt attttttgaa atctcgcatg aactggatat
     4501 tttggatatt gtttttaaac ttgcttatgt taggcattag tctaatcgat tatgatt
//
LOCUS       DAHHSC010000186         4534 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.187, whole genome shotgun sequence.
ACCESSION   DAHHSC010000186 DAHHSC010000000
VERSION     DAHHSC010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 4534)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4534)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4534
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.187"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            <1..573
                     /locus_tag="NBI57_002229"
     CDS             <1..573
                     /locus_tag="NBI57_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HCD1823158.1"
                     /translation="NDSFTYNLIDYIKTQTKLTVQVVGIDNLLIEDVINMKPKAIVIS
                     PGPGNPDDYPILNEVLEQFYQRVPILGVCLGFQCIVSYFGGNIIHGYHPVHGHTTQLR
                     HTNEGIFQGLPQNFNVMRYHSLIADGATFPNCLKITAKNDEAIIMAFEHIRFPVFGVQ
                     YHPESILSEYGYRQVELFLSKVGDYCENRI"
     gene            557..1708
                     /locus_tag="NBI57_002230"
     CDS             557..1708
                     /locus_tag="NBI57_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I family
                     protein"
                     /protein_id="HCD1823159.1"
                     /translation="MRIEYNYRYYLTENEYKQYHIQLKGFIKKYVATKLADVGEVIHF
                     AQAQQRQGRYVSLYLSYEAAKYFNHVMCTHSLAKDDIYAVAYSFEKAESINSTYEHQT
                     SYVSKHHFSFVESSEVMMTNIKRVQQAIVEGETYQVNYTARLTDNIYYPISTLYERLT
                     QFSNGNYTALLQTDEIQVASISPELFFQKGQFNNVDNVIISKPMKGTMPRGKTEAEDQ
                     QYYKTLQTSSKDRAENVMIVDLLRNDIGRISQSGSIKVYKLFFIEAYKTVFQMTSMVS
                     GTLKTNTDLTQILTSLFPCGSITGAPKLNTMKYIKQFESSPRGIYCGAIGLLLPTEDD
                     KMIFNIPIRTIEYKYGQAIYGVGAGITIDSKPKDEVNEFYAKTKILEML"
     gene            1708..2316
                     /locus_tag="NBI57_002231"
     CDS             1708..2316
                     /locus_tag="NBI57_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HCD1823160.1"
                     /translation="MQLFETMKIDNGHIPRLTYHTNRIKCSSERLNFKFDEHAWRNEL
                     NDVTTKYHSGQYRLKIVLNAESKFETIVSPLPEKSSFTAKFQVLPKVVNPTFIKNKTT
                     ERKHLAHNHETDLILLTSEDGKVLEFDIGNIVIEEDGKWYTPSYKDDFLKGCMRDYLI
                     DSDKLVEKDFNKNELIYKYHNNEIRLFLINSLREVADVHLCL"
     gene            2382..2588
                     /locus_tag="NBI57_002232"
     CDS             2382..2588
                     /locus_tag="NBI57_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1823161.1"
                     /translation="MIIVYIVLLLILVYVNYRLVNRLLSENRIYVVRLIVTITTVISF
                     ILVYALIHELMPFVVRAMDLMYHQ"
     gene            2676..3386
                     /locus_tag="NBI57_002233"
     CDS             2676..3386
                     /locus_tag="NBI57_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="allophanate hydrolase subunit 1"
                     /protein_id="HCD1823162.1"
                     /translation="MKIYSQGDQAIVVAIEKEVSKSLTEDLLTLRSYLIEQNYPFIIE
                     IVPSESDMMIVYDARDMIKHHNIQSPFLYMKALIESIHLNIKHDFNQQDLIEIPIVYG
                     SKYGPDIESLLKHYKIKLETFIELHSKTQYFVSMMGYSPGFPYLTGLNKKLYINHTSK
                     QKKFIPAGSVVLEGKKCGIVTTDTINDWLVIGYTPLSLFNPKESDFARLKLGDNIKFR
                     PINENELEVGAFKDVNHN"
     gene            3370..4374
                     /locus_tag="NBI57_002234"
     CDS             3370..4374
                     /locus_tag="NBI57_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-dependent carboxyltransferase family
                     protein"
                     /protein_id="HCD1823163.1"
                     /translation="MSIIIEKSGLFSSFQDFGRRGYEHDGVIPCGALDTLAHEIANRL
                     VANDKNEATLEMTNKMATIRFTEPTLIALAGGNVKAYTEHMTISPYKLYLLDKGDVLK
                     FRETSYTSRVYLAVGGGFELDAWLGSNSTDFNVKIGGFKGRTLQDGDEIKLKRDYTAR
                     HHKLFENLAHTKQTDWGIDGYALSFNYMSDVFHVVKNKGTEDFKEDAIQRFVKHDYKV
                     TSKANRMGMMLEGEKIKAFYEDMPPYQTVKKGTIQIKRDGTPIILLNDHYTLGSYPQI
                     GTIASYHLTKLAQKPQGSRLKFQFIDILTAEKNLVKYSNWLNQLFHGIEYRMQLEMMK
                     "
BASE COUNT         1651 a          629 c          818 g         1436 t
ORIGIN      
        1 aatgattcat ttacatataa tttaatagac tatattaaga ctcaaacgaa actaacagtt
       61 caagttgttg gtattgataa tctgctgata gaagacgtca ttaatatgaa gccaaaagca
      121 attgttattt cgcctgggcc gggtaatccg gatgattatc ctatcttgaa tgaagtgtta
      181 gaacaatttt atcagcgtgt acctatacta ggtgtatgtt taggatttca atgtatcgtg
      241 tcttattttg gtggaaatat cattcacggc tatcatcctg tacacggaca tactacacag
      301 ttacgccata ccaatgaagg tatttttcaa ggactgcctc aaaatttcaa tgtaatgcgt
      361 tatcattcat taattgctga cggagcgact tttccaaatt gcttaaagat tacagcaaaa
      421 aacgatgaag cgattattat ggcatttgag catattagat ttccggtttt tggtgtgcaa
      481 tatcatcctg aatctatttt gagtgaatac ggttatcgac aagttgaatt atttttatcg
      541 aaggtaggtg attactgtga gaatagaata taattatcgc tactatttaa ctgaaaatga
      601 atataagcaa taccatattc aattaaaggg atttataaag aagtatgttg ctactaagtt
      661 ggctgatgtg ggagaagtga tacactttgc acaagcgcag caacgacaag gtagatatgt
      721 ctcgttatat ttaagttacg aagcggcaaa gtattttaat catgttatgt gtacacattc
      781 attagctaaa gatgatattt atgcagtagc ttatagtttt gaaaaagcgg aaagcataaa
      841 ttcaacatat gaacatcaaa cttcttatgt atcaaagcat catttttcat ttgttgaatc
      901 ttctgaggtt atgatgacta atattaaacg tgtccaacaa gcaattgttg aaggtgaaac
      961 gtatcaagtg aactatacgg cgcgcttaac ggataacatt tattatccta ttagtacttt
     1021 atatgaacga ttaactcaat ttagtaatgg taattatact gcgttattac aaactgatga
     1081 aatccaagta gcgtctatct caccagaatt attttttcaa aaaggacaat ttaacaatgt
     1141 cgataacgtt atcataagca aaccgatgaa agggacaatg cctagaggta aaacggaagc
     1201 tgaagatcaa cagtattata aaacattgca aacttcttcg aaagatcgtg cagaaaatgt
     1261 catgattgtt gatttgctaa gaaacgatat agggagaata tcacagagtg gctcaattaa
     1321 ggtgtataaa ctatttttta ttgaggcata taaaactgta tttcaaatga cttcgatggt
     1381 aagtggaact ttaaaaacta atacagactt aactcaaatt ttaacatcgt tatttccttg
     1441 tggttcgatt acaggtgcac cgaaactgaa tacaatgaaa tatattaaac aatttgaaag
     1501 ttcacctcgt ggtatatact gcggagcaat tggactatta cttccaactg aagatgataa
     1561 aatgattttt aatattccga ttcgtactat tgagtataaa tatggacaag cgatttatgg
     1621 agtcggagca ggtattacaa ttgattctaa gccaaaagat gaagtaaatg aattttacgc
     1681 aaaaaccaag attttggaga tgttataatg caattatttg aaacaatgaa aattgataat
     1741 ggacatatcc ctagacttac ttatcatact aatcgcataa aatgttcttc tgagcgatta
     1801 aactttaaat ttgatgaaca tgcatggcga aatgaattaa acgatgtaac aacaaagtat
     1861 cacagtggtc aatatagact taaaatcgta ttaaatgctg aaagcaaatt tgaaacgata
     1921 gtgtcacctt tacctgagaa aagtagtttt acagcaaaat ttcaagtgtt gcccaaagta
     1981 gttaatccaa cttttataaa aaataaaacg acagaacgaa agcatttagc acacaatcat
     2041 gaaacagatt taatattgct aacttcagag gacggcaagg tccttgaatt tgatattggc
     2101 aacattgtca ttgaagagga tggaaaatgg tacacaccaa gttataaaga tgatttctta
     2161 aaaggatgca tgcgtgatta tttaatagat agtgacaaac ttgttgaaaa agactttaat
     2221 aaaaacgaat tgatttataa atatcataac aatgagatac gtttattttt gataaatagt
     2281 ttacgagagg ttgccgatgt ccacctttgc ctttaaaatt agtatgataa taaagtagaa
     2341 aatgatgtga aaacaaattt aaaaattaaa tggggccaaa tatgattatt gtttatattg
     2401 tgctgttgtt aattcttgta tacgtaaatt atcgattagt gaatcgattg ctatctgaaa
     2461 atagaatata tgttgttcgt ttgatagtaa caattactac tgttataagc tttatccttg
     2521 tatacgcatt aattcacgaa cttatgcctt ttgttgtgcg ggcaatggat ttaatgtacc
     2581 accagtaaat ttagtgagta ggtaaaacga actattcaat cgattctata attaaaaatg
     2641 aaagtaaagt aaccaaatat tgaagaggtg tacaaatgaa aatatatagt caaggtgacc
     2701 aagccattgt agtcgcaatt gaaaaagaag tatctaaaag tttaaccgaa gatttattaa
     2761 cacttcgctc atatttaatt gaacaaaatt atccatttat tatagaaatt gtgccatcag
     2821 aatcagacat gatgattgtc tatgacgcaa gggatatgat taaacaccat aatatacaat
     2881 cacctttttt atacatgaaa gcactaatag aatcgattca tttaaacata aaacatgatt
     2941 ttaaccagca agatttgatt gaaataccaa ttgtgtatgg ttcgaaatat ggtccggata
     3001 tagaatcact tttaaaacat tacaaaatca agctagaaac ttttattgaa ttacattcta
     3061 agacgcaata ttttgtttcg atgatgggat attcacctgg gtttccttat ttaactggat
     3121 taaataagaa attgtatatt aatcacacga gtaaacagaa aaaattcatt ccagctggtt
     3181 ctgtagtact tgaagggaaa aaatgcggta ttgtaacaac ggatacaatt aatgattggt
     3241 tagttattgg ttatacacca ttatcacttt ttaatccgaa agaatcagat ttcgcacgct
     3301 taaagttagg cgataatatt aaatttagac ctatcaatga aaatgaatta gaagtaggag
     3361 cgtttaaaga tgtcaatcat aattgaaaaa agtggcttat tcagtagctt tcaggacttt
     3421 ggtagaaggg gatatgaaca tgatggtgta attccatgtg gtgcacttga tactttagca
     3481 catgaaattg ctaatcgatt agttgctaat gacaagaatg aagcaacttt ggaaatgact
     3541 aataaaatgg caacgattcg ttttacagaa cctacgctga ttgcattagc agggggtaat
     3601 gtcaaagctt acactgagca tatgactata tctccatata aattgtattt gttagataaa
     3661 ggcgatgttt taaagtttag agaaacaagt tatacatcgc gagtgtattt agctgtggga
     3721 ggcggatttg aattagatgc atggttagga tctaactcaa ccgactttaa tgtaaaaatt
     3781 ggtggtttta aaggtagaac attacaagat ggcgatgaaa taaagcttaa gagagattat
     3841 acagctcgtc atcataagtt atttgaaaac cttgctcaca cgaaacaaac agattggggt
     3901 attgatggat acgccttgtc atttaattat atgtctgatg tatttcatgt cgttaaaaat
     3961 aaaggtacgg aagattttaa agaagatgcc attcaaagat ttgtgaaaca tgattataaa
     4021 gtaacgagca aagcaaatcg catgggaatg atgcttgaag gtgaaaaaat caaagctttt
     4081 tatgaagata tgccgccgta tcagactgtc aaaaaaggaa cgatacaaat taagcgtgat
     4141 ggcacaccta ttatcttatt aaatgatcat tatacgttag gtagctaccc gcaaatcggt
     4201 acaatcgcaa gttatcattt aacgaaatta gcacaaaaac cgcaaggatc acgtttgaaa
     4261 tttcaattta tagatatttt aacggctgaa aagaaccttg ttaagtatag taactggtta
     4321 aaccaattat tccatggaat agaatataga atgcaattag aaatgatgaa ataatatttg
     4381 gtacgtagct catactcgag tccgatgcaa ataatttcct cctaatgtat aatgaaataa
     4441 tactgtgttt tatctgcgaa atgtatcatt ttctaattcg tttcacagta aaatgaaaag
     4501 ataaagtgtg tttttacttg aattttgact aaaa
//
LOCUS       DAHHSC010000187         4454 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.188, whole genome shotgun sequence.
ACCESSION   DAHHSC010000187 DAHHSC010000000
VERSION     DAHHSC010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 4454)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4454)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4454
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.188"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            86..769
                     /locus_tag="NBI57_002235"
     CDS             86..769
                     /locus_tag="NBI57_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HCD1823164.1"
                     /translation="MEWILFDKDGTLIEFDRSWEKIGVRFVQSLLETFPVHNKEAALR
                     QLGVIKESIDPKSVMGSGSLQQIIQAFNDVTGQDTTDWSKSTSQKLVDERIPEINWVE
                     GVKEALIDLKAKGYQLGIVTSDTKKGVEQFLAHTNAISLFDLIISTEADAYEKPNPKV
                     LSPLFEQYNVDPQKVAIVGDTANDMKTASNANLGMAIGVLTGIATKEELHEADIILNS
                     AADILEALN"
     gene            complement(885..1547)
                     /locus_tag="NBI57_002236"
     CDS             complement(885..1547)
                     /locus_tag="NBI57_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HCD1823165.1"
                     /translation="MNNYGIPQNAIITIAGTVGVGKSTLTQALADKLNFKTSFENVEH
                     NPYLDKFYSDFERWSFHLQIYFLAERFKEQKRMFEYGGGFVQDRSIYEDVDIFAKMHE
                     EEGTMSKEDFKTYSDLFNAMVMTPYFPKPDVMIYLECNYDEVIDRIIERGREMEINTD
                     PEYWKKLFKRYDDWINSFNACPVVRININEYDIHKDPESLNPMIDKIARIIQTYRQVD
                     TR"
     gene            complement(1540..2157)
                     /locus_tag="NBI57_002237"
     CDS             complement(1540..2157)
                     /locus_tag="NBI57_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HCD1823166.1"
                     /translation="MNKPFIAIEGPIGVGKSSLAHKLSQTLDFYEEKEIITENPFLSD
                     FYEDISKWSFQTEMFFLCNRYKQFQDVTQLNQGVVSDYHIHKNKIFAKNTLSPVEFQK
                     FSKIYDILTEDMIMPNMIIFLDADLDVLKSRIAKRNRSFEHQIEDEYLLKLKKDYREY
                     YESLQSNGSNVVLIDTTSIDFLKNEQDYEDILHIILPMIGDITNE"
     gene            2224..2694
                     /locus_tag="NBI57_002238"
     CDS             2224..2694
                     /locus_tag="NBI57_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HCD1823167.1"
                     /translation="MTNDIYFMTLAIEEAKKAAQLGEVPIGAIITKDDEVIARAHNLR
                     ETLQQPTAHAEHIAIERAAKMLGSWRLEGCTLYVTLEPCVMCAGTIVMSRIPRVVYGA
                     DDPKGGCSGSLMNLLQQSNFNHRAIVDKGVLKEACSTLLTTFFKNLRANKKSTN"
     gene            2841..3710
                     /locus_tag="NBI57_002239"
     CDS             2841..3710
                     /locus_tag="NBI57_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HCD1823168.1"
                     /translation="MIKLIATDMDGTLLNAAHEISQPNIDAIKYAQEQGITVVIATGR
                     AFYEAQAPVADTDLTVPYICLNGAEVRDETFNVMSTSHLNKSLVHKITNVLKDAGIYY
                     QVYTSRAIYTEDPQRDLDIYIDIAERAGQHANVERIKNGIQRRIDNGTLKVVDNYDAI
                     ENIPGELIMKILAFDGNLEKIDKASKILAESPNLAISSSSRGNIEITHSDAQKGIALE
                     TIAERLGIEMKDVMAIGDNLNDLSMLEKVGYPVAMENGAEEVKKIAKYVTDTNENSGV
                     GKAIMKLLREQQV"
     gene            3732..4298
                     /locus_tag="NBI57_002240"
     CDS             3732..4298
                     /locus_tag="NBI57_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HCD1823169.1"
                     /translation="MKGLIIIGSAQVNSHTSALARYLTEHFKTHDIEAEIFDLAEKPL
                     NQLDFSGTTPSIDEIKQNMKDLKEKAMAADFLILGTPNYHGSYSGILKNALDHLNMDY
                     FKMKPVGLIGNSGGIVSSEPLSHLRVIVRSLLGIAVPTQIATHDSDFAKNEDGSYYLN
                     DSEFQLRARLFVDQIVSFVNNSPYEHLK"
BASE COUNT         1625 a          629 c          775 g         1425 t
ORIGIN      
        1 caatactacg agttcatttt caggggataa cgaatatcat agaatcagca taatacgaaa
       61 taaataaata ggagtattga tatcaatgga atggatatta tttgataaag atggtacgtt
      121 aattgaattt gatagaagtt gggaaaaaat aggggtacga tttgtacaat cattgcttga
      181 gacttttcca gtacataata aagaagctgc tttaagacaa ctcggtgtca ttaaagaatc
      241 tattgatcca aaatcagtga tgggttcagg atctttacaa caaattatcc aggcatttaa
      301 tgatgtgacg ggacaagata caaccgactg gtccaagtca acaagtcaaa agctggtaga
      361 tgaacgtatt cctgaaatta attgggtaga aggtgttaaa gaagcactta tcgatttgaa
      421 agcaaaaggc tatcaacttg gtattgttac gagtgatact aaaaaaggtg tagaacaatt
      481 tttagcacat accaatgcta tctcgttgtt cgatttgatc atttctaccg aagcggatgc
      541 ctatgagaag ccaaatccta aagtattatc gcctttattt gagcaatata atgtagatcc
      601 tcagaaagta gctatagtag gagacactgc taatgatatg aagacagcaa gtaatgcaaa
      661 tttaggtatg gcaataggtg tattaacagg tattgcaaca aaagaagaat tacatgaagc
      721 tgatattatt ttaaatagtg cggcagatat tttagaagct ttaaattaaa aagaaagaca
      781 tagtgtatat gtgaattaat gtattcgata gaattgataa tgtaatcgat gatatctatg
      841 gaatatacat tgaatataaa catatacgct atgtctttag tcttttatcg tgtatctact
      901 tgtcgatatg tttgaataat tcgagcaatt ttgtctatca taggatttaa agattcgggg
      961 tccttatgga tatcatattc attaatattg atacgtacaa ctggacatgc attaaagcta
     1021 ttaatccaat cgtcatagcg tttaaatagc tttttccagt attcagggtc tgtattaatt
     1081 tccatttcgc gaccacgttc aataatacga tcaatgacct catcatagtt acattctaaa
     1141 taaatcatta catctggttt aggaaaataa ggtgtcatga ccatggcatt aaataagtct
     1201 gaatatgttt tgaaatcttc tttactcatt gtgccttctt cttcatgcat ttttgcaaaa
     1261 atatcaacat cttcataaat tgatcgatct tggacaaagc caccaccata ttcaaacata
     1321 cgcttttgtt ctttaaaacg ttcagctaag aagtaaattt gcaaatggaa actccatcgt
     1381 tcaaaatcgc tataaaattt atctaaatat ggattatgtt cgacattttc aaaagacgtt
     1441 ttaaagttta atttatctgc aagtgcttgc gttagtgttg attttccaac accaactgta
     1501 cctgcaatgg ttataatggc attttgtgga ataccgtaat tattcattgg taatatctcc
     1561 tatcataggt aatataatat gtaatatatc ttcgtaatct tgttcatttt taagaaaatc
     1621 aatagaagtt gtatcgatta aaactacatt tgaaccatta ctttgtaagg actcataata
     1681 ctcacgataa tcttttttta actttaacag atattcatct tctatttgat gctcaaaact
     1741 acggttacgt ttagcaattc tagattttaa cacatcaagg tctgcatcta aaaagataat
     1801 catattcggc ataatcatat cttcagttaa aatatcataa attttactga atttctgaaa
     1861 ttcaacagga ctcaaagtat ttttagcaaa tatcttattt ttatgtatat gataatcact
     1921 aactacacct tgatttagtt gtgttacatc ttgaaattgc ttatatctat tgcataaaaa
     1981 gaacatttca gtttgaaaac tccatttaga gatatcttca taaaagtctg ataaaaatgg
     2041 attttctgtg atgatttctt tttcttcata aaaatctaaa gtttgactta atttgtgtgc
     2101 aagtgaagat ttacctacgc caataggacc ttcaattgct ataaaaggtt tgttcatatt
     2161 cacatctcca aatgttaaaa tagacattga gtattgtacc ataagtgagg tatctggaca
     2221 taaatgacaa atgatatata ttttatgaca ttagcgattg aagaagctaa aaaagcagct
     2281 caactaggcg aagtacctat aggtgctatc atcactaaag atgatgaagt tatcgctaga
     2341 gcacataatt taagagaaac actacaacaa ccaacggcgc atgctgaaca tattgcaatt
     2401 gaacgtgcag ccaaaatgtt aggtagttgg cgtttagaag gttgcacatt atatgtaacc
     2461 ttagaaccat gtgtcatgtg cgcaggaaca attgtaatga gtcgcattcc aagagtcgtc
     2521 tatggcgcag atgatcctaa aggtggttgt agtggcagtt taatgaattt attgcaacaa
     2581 tctaatttta atcatcgtgc aattgttgat aaaggtgtac ttaaagaagc atgtagcaca
     2641 ttattaacaa cattttttaa aaacttaaga gccaataaga aatccaccaa ttagacgata
     2701 gtacatacta attaaaattt gttagaatta cattatatga taaattaatg acaatataaa
     2761 tgttaataga aaagtatgtg cgctgagtat atattcttat ttaagaacaa gattttaaat
     2821 ttacgaaacg aggtaacata atgataaaac taatagccac tgatatggat ggcacgctac
     2881 ttaatgcagc acatgaaatt tctcaaccta atattgatgc gattaaatac gctcaagaac
     2941 aagggataac ggttgttatc gcgacaggtc gagcatttta tgaagcacaa gcaccagttg
     3001 ctgacacaga tttaacagta ccatatattt gtttgaatgg tgctgaagta cgtgatgaaa
     3061 ctttcaatgt aatgagcact tcacacctta ataaatcgtt agtacacaaa attacaaatg
     3121 ttttaaaaga tgcaggtatt tattatcaag tatacacgag tcgtgcgatt tatactgaag
     3181 atccacaaag agatttagac atttacatag atattgctga gcgtgcaggt caacatgcaa
     3241 acgttgagcg tattaaaaat ggtattcaaa gacgcataga taatggtacg ttgaaagttg
     3301 ttgataatta tgatgctatt gaaaacatac ctggtgaatt aattatgaaa atattagcat
     3361 ttgatggaaa tttagaaaaa attgacaaag ctagtaaaat tttagctgaa tctccgaatt
     3421 tagctatatc atcatcttcg agaggaaata tagaaataac gcattcagat gcacaaaaag
     3481 gtattgcgct agaaacaatt gccgaaagat tagggattga aatgaaagat gtcatggcaa
     3541 taggtgacaa tttaaatgac ttatcaatgt tagagaaagt tggctatcca gttgcgatgg
     3601 aaaatggtgc agaagaagtt aaaaaaatag cgaaatatgt cacagatacg aatgaaaata
     3661 gtggtgttgg aaaagctatt atgaaattat tacgtgaaca acaagtttaa taaaaaaaag
     3721 aggggtcaaa tatgaaagga ttaattatta ttggcagtgc acaagtgaat tcacatacaa
     3781 gtgcactagc aagatactta actgagcatt ttaaaacaca tgatattgaa gcggaaatat
     3841 tcgatttagc agaaaaaccg ttaaatcaat tagatttttc aggaacaaca ccgtctattg
     3901 atgaaatcaa acaaaatatg aaagatttaa aagagaaagc aatggcggcg gactttttaa
     3961 tattaggaac gccaaactat catggttcat attctggaat attgaaaaat gcattagatc
     4021 atctaaatat ggattatttt aaaatgaaac ctgtaggctt aataggaaat agtggtggta
     4081 ttgttagttc agagccattg tcacatttaa gagtaatcgt cagaagttta ctaggcattg
     4141 ctgtaccaac tcaaatagca acacatgatt ctgattttgc taaaaatgaa gatggttcat
     4201 attacttaaa tgatagtgaa ttccaattac gagcaagatt atttgtcgat caaattgtat
     4261 cttttgtgaa taatagtcca tatgaacatt taaaataata ttaaaaaata tgtaaatata
     4321 cattaaaaat aggtatgtag cttaaggtat ttcattgaga atgcttttaa gtcacatgcc
     4381 tttttttgtt ctaatgcacc aatagtaagc ctttgagatt actgttgtga gcaatgttat
     4441 taattaaaat aaga
//
LOCUS       DAHHSC010000188         4430 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.189, whole genome shotgun sequence.
ACCESSION   DAHHSC010000188 DAHHSC010000000
VERSION     DAHHSC010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 4430)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4430)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4430
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.189"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            483..1259
                     /gene="sed"
                     /locus_tag="NBI57_002241"
     CDS             483..1259
                     /gene="sed"
                     /locus_tag="NBI57_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_128623913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphylococcal enterotoxin type D"
                     /protein_id="HCD1823170.1"
                     /translation="MKKFNILIALLFFTSLVISPLNVKANENIDSVKEKELHKKSELS
                     STALNNMKHSYADKNPIIGENKSTGDQFLENTLLYKKFFTDLINFEDLLINFNSKEMA
                     QHFKSKNVDVYAIRYSINCYGGEIDRTACTYGGVTPHEGNKLKERKKIPINLWINGVQ
                     KEVSLDKVQTDKKNVTVQELDAQARRYLQKDLKLYNNDTLGGKIQRGKIEFDSSDGSK
                     VSYDLFDVKGDFPEKQLRIYSDNKTLSTEHLHIDIYLYEK"
     gene            1854..2891
                     /locus_tag="NBI57_002242"
     CDS             1854..2891
                     /locus_tag="NBI57_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001645037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4238 domain-containing protein"
                     /protein_id="HCD1823171.1"
                     /translation="MALGILSKLAMAGIVLTSVTGAATLGGVSSIAHEVHAEENANQE
                     HFEKTLEGKVLNFKDVLSKNTISIKDFSKAVENELKNKGFDPSEYKLKFKVKVGHSVY
                     QSSDGGNLIVGNEHIGTATAEFNPTKHKLGVYESEIEKIIDYIIKNITSKDLDVSVNM
                     QNKIFQYIHLQYLRTDAGRINFMNLIENPFTYKPRKKPIDLDEIQKHKNTIIKFNEIF
                     KQGNNLENLLKRMKKPSNMNFHIAISEDNLLTSDNPVIATDNWNQIMLPITPNILIEF
                     QEDKINSSNDLRVILKKNKTRYVNEATINTANYFIISNKEFTRYQYKYIDNRFNNKNW
                     EIGYPHVNLKN"
     gene            complement(3094..3189)
                     /locus_tag="NBI57_002243"
     CDS             complement(3094..3189)
                     /locus_tag="NBI57_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HCD1823172.1"
                     /translation="MFSTLFVTIIAPIVVGVILTLFSHWLNNREK"
     gene            complement(3454..3903)
                     /locus_tag="NBI57_002244"
     CDS             complement(3454..3903)
                     /locus_tag="NBI57_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002471088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1823173.1"
                     /translation="MVSYIFYFATEPEISSREFDFSVLIIMLCIAFRNYFRGYGFAIH
                     LTSDLFFEMTLLLLPVSLLLLNKKTNEEIANTLKSNIPLIFPILGGMFYIGIGRFLIY
                     LAHKIPIIDFIFSLFGLVMLIAGIINILISILIFVTKQRVKQSASKK"
     gene            3961..>4430
                     /locus_tag="NBI57_002245"
     CDS             3961..>4430
                     /locus_tag="NBI57_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_064305258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS257 family transposase"
                     /protein_id="HCD1823174.1"
                     /translation="MNYFRYKQFNKDVITVAVGYYLRYALSYRDISEILRERGVNVHH
                     STVYRWVQEYAPILYQIWKKKHKKAYYKWRIDETYIKIKGKWSYLYRAIDAEGHTLDI
                     WLRKQRDNHSAYAFIKRLIKQFGKPQKVITDQAPSTKVAMAKVIKAFKLKPDCH"
BASE COUNT         1726 a          587 c          679 g         1438 t
ORIGIN      
        1 aataaaatta ctacgaatta ataattatta aaatatttat ttaaaaaata tttaaataaa
       61 tataaagtat atttaaaatt ttattaatga tggtttcttc gataaaatgt tttaattatt
      121 aagagagact tgatctgttt agaccaagtc tcaaattatt aaagataata tttgttggag
      181 tgtatgaaat ggatcaaata tattgatata atgaaagtga gcaagttgga tagattgcgg
      241 ctagtctctt gaaaaaaggg attcctatgt ttatagttgg tacttctagg aaggactagc
      301 atgtcttatt tcgaaatgct gatggttgta ttaacaatta tgagtttagt cttaattagt
      361 aatcaaaact ctgaatgtat tgtctacata atcggtgtaa caataatata tatccgaaag
      421 attaaagtac tctataaaag aatcatttaa aggtgctcaa atttattgaa aaaaggtgga
      481 atatgaaaaa atttaacatt cttattgcat tactcttttt tactagtttg gtaatatctc
      541 ctttaaacgt taaagccaat gaaaacattg attcagtaaa agagaaagaa ttgcataaaa
      601 aatctgaatt aagtagtacc gcgctaaata atatgaaaca ttcttatgca gataaaaatc
      661 caataatagg agaaaataaa agtacaggag atcaattttt agaaaatact ttgctttaca
      721 aaaaattttt tactgacctt atcaattttg aagatttatt aataaacttc aattcaaaag
      781 aaatggctca acatttcaaa tctaaaaatg tagatgttta cgctataaga tatagcatta
      841 attgttatgg tggtgaaata gataggactg cttgtacata tggaggtgtc actccacacg
      901 aaggtaataa attaaaagaa cgaaaaaaaa taccaatcaa tttgtggata aatggtgtac
      961 aaaaagaagt ttctttagat aaagttcaaa cagataaaaa aaatgttacc gtacaagaat
     1021 tagatgcaca agcaaggcgc tatttgcaaa aggatttaaa attgtataat aatgatactc
     1081 tcggaggaaa aatacagcgc ggaaaaatag agtttgattc ttctgatggg tctaaagtct
     1141 cttatgattt atttgatgtt aagggtgatt ttcccgaaaa acaattacga atatacagtg
     1201 ataataaaac attatccaca gagcaccttc atattgacat ctatttatat gaaaagtagt
     1261 caattcaaat actttaacat cagacactaa agccatttca aagaatgcta aagatttatt
     1321 aatcggtatc gaaaaaattc aggagactta ctagataaag caaaagaaag agctaataat
     1381 ctaaatattg aagaaaacag aaacttataa aaatatgggg tggaatttta caaattatac
     1441 aagctatcat caatttcttt tcaagcttat ttaatcgtct tttaataaat gtgtaggaga
     1501 ttttagatgc tatcaataac atagtcttaa agagagttgt aacgaacgta ataaattata
     1561 atagtagttt acctgggtcg aataaaacga tatgcaatgc atttgttgat gacaaaaaat
     1621 gtattacata tctaaattaa tccatagaaa atgccaattc ttgatataat atcactttgc
     1681 aacagaaccg ttgccccatc tcaagtgcct tctacttact tttaatattc atttaatata
     1741 ttgttgatga atttttaatt gtaatataca ttatttaact taataattat atttcatcat
     1801 tatgtgagcg cttttagcga acaaaaatta acttagacta gagatggata aatatggctt
     1861 taggtatatt atcaaaattg gcaatggcag gtattgtttt aactagcgtt acaggcgctg
     1921 caactctagg aggcgtttct agtattgctc atgaagtaca tgcagaggaa aacgctaatc
     1981 aagaacactt tgagaaaact ttagaaggta aggtattaaa tttcaaagat gtattgagta
     2041 aaaatacaat atcaataaaa gacttcagta aagcagttga aaatgaattg aaaaataaag
     2101 gctttgaccc tagtgagtat aaacttaaat ttaaggtaaa agtaggacat agtgtgtatc
     2161 aaagtagtga tggaggaaat ttaattgttg gtaatgaaca tattggcact gcaactgccg
     2221 aattcaatcc tactaaacac aaattagggg tatatgaatc tgaaatagaa aagattattg
     2281 attatattat taaaaatatt acatctaagg acttagatgt ttcagtaaat atgcaaaaca
     2341 agatttttca atatattcat ttacaatatt tacgtactga cgccggaaga attaatttta
     2401 tgaatttaat tgaaaatccg tttacatata aacctagaaa aaaacctatc gatctagatg
     2461 aaatacaaaa acacaaaaat acaataataa agtttaatga aatttttaag caaggcaaca
     2521 acctagaaaa cttattaaaa agaatgaaaa aaccatctaa catgaatttt catattgcaa
     2581 taagtgaaga taatttatta actagtgata atccagtaat cgctactgat aattggaacc
     2641 aaataatgct tccaattact ccaaatattc ttatagaatt tcaagaagat aaaattaact
     2701 catctaacga tttaagagta atattaaaaa aaaataaaac aagatatgta aatgaagcaa
     2761 caattaatac agcaaattat tttattatat caaataaaga gtttacccgt tatcaatata
     2821 aatatataga caatcgcttt aataataaga attgggagat tggttatcct catgtaaatt
     2881 tgaaaaatta aaaacttttc tagcattata catattacaa cttttgttcc ttctttttaa
     2941 tactattgaa agattgtttt ttgcatttct ccactaacta gtgttataat ttagccaaca
     3001 aatagtgtac tagcatgata aacaccgagc ccgataacta ctgcgaatag ttatcaggct
     3061 cttttggtgt attaactata tgtcgtcgct aaattacttc tcacgattat tcagccaatg
     3121 agaaaataat gtgagtataa ctcctactac gataggagct ataatagtta caaatagtgt
     3181 actgaacatg atgtcacctc cttccatgct aaatacagca cagaagtaaa cgacgacctt
     3241 tgtattatat catttctaat aattgagtta cagataactt ccgataagtt aaattatgtt
     3301 aactaaacca aaagggacaa acgccgtcat ttcagcgttt gtccctttgt gtttggatta
     3361 tcgattattc atattttata cataccctta accacacgtt ttcatgtatt ttcattaaga
     3421 gaatttttgt atctttattc agttgaagag cgtctatttt ttagacgctg attgttttac
     3481 gcgttgtttt gtaacgaata ttaatattga aattaaaatg tttataatac cagcaattaa
     3541 catgactaat ccaaaaagac taaatatgaa gtcaatgatt gggatcttgt gagcgagata
     3601 tattaaaaat ctccctatac ctatataaaa cataccccct aaaattggaa agatgagagg
     3661 tatattactt tttaatgtat tagcgatttc ttcatttgtc tttttattta agagtaaaag
     3721 tgatactggg agtaatagca atgtcatttc aaaaaataag tcggaagtta aatgaatagc
     3781 aaaaccataa cctctgaagt aatttctaaa tgcgatacat aacataatga ttaaaactga
     3841 gaaatcaaac tctctactac taatttcagg ttctgttgca aagtaaaaaa tatagctaac
     3901 cactggttct gttgcaaagt tgaatttata gtataatttt aacaaaaagg agtcttctgt
     3961 atgaactatt tcagatataa acaatttaac aaggatgtta tcactgtagc cgttggctac
     4021 tatctaagat atgcattgag ttatcgtgat atatctgaaa tattaaggga acgtggtgta
     4081 aacgttcatc attcaacggt ctaccgttgg gttcaagaat atgccccaat tttgtatcaa
     4141 atttggaaga aaaagcataa aaaagcttat tacaaatggc gtattgatga gacgtacatc
     4201 aaaataaaag gaaaatggag ctatttatat cgtgccattg atgcagaggg acatacatta
     4261 gatatttggt tgcgtaagca acgagataat cattcagcat atgcgtttat caaacgtctc
     4321 attaaacaat ttggtaaacc tcaaaaggta attacagatc aggcaccttc aacgaaggta
     4381 gcaatggcta aagtaattaa agcttttaaa cttaaacctg actgtcattg
//
LOCUS       DAHHSC010000189         4394 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.190, whole genome shotgun sequence.
ACCESSION   DAHHSC010000189 DAHHSC010000000
VERSION     DAHHSC010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 4394)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4394)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4394
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.190"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            <1..368
                     /locus_tag="NBI57_002246"
     CDS             <1..368
                     /locus_tag="NBI57_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016912861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase family
                     protein"
                     /protein_id="HCD1823175.1"
                     /translation="LWNRDVGNIYTGSLYLSLISLLQNHTFQPEEKVCLFSYGSGAVG
                     EIFSGSIVKGYDKALDKEKHLNMLESREQLSVEEYETFFNRFDNQEFDFERELTQDPY
                     SKVYLYSIEDHIRTYKIEK"
     gene            1245..1988
                     /locus_tag="NBI57_002247"
     CDS             1245..1988
                     /locus_tag="NBI57_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001830227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphoryl diester phosphodiesterase"
                     /protein_id="HCD1823176.1"
                     /translation="MLTVYGHRGLPSKAPENTIASFKAASEVEGINWLELDVAITKDE
                     QLIIIHDDYLERTTNMSGEITELNYDEIKDASAGSWFGEKFKDEHLPTFDDVVKIANE
                     YNMNLNVELKGITGPNGLALSKSMVKQVEEQLTNLNQNQEVLISSFNVVLVKLAEEIM
                     PQYNRAVIFHTTSFREDWRTLLDYCNAKIVNTEDAKLTKAKVKMVKEAGYELNVWTVN
                     KPARANQLANWGVDGIFTDNADKMVHLSQ"
     gene            2085..2513
                     /locus_tag="NBI57_002248"
     CDS             2085..2513
                     /locus_tag="NBI57_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000872606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HCD1823177.1"
                     /translation="MKYDDFIVGETFKTKSLHITEEEIIQFATTFDPQYMHIDKEKAE
                     QSRFKGIIASGMHTLSISFKLWVEEGKYGEEVVAGTQMNNVKFIKPVYPGNTLYVIAE
                     ITNKKSIKKENGLVTVSLSTYNENEEIVFKGEVTALINNS"
     gene            complement(2559..>4394)
                     /gene="mec"
                     /locus_tag="NBI57_002249"
     CDS             complement(2559..>4394)
                     /gene="mec"
                     /locus_tag="NBI57_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001801873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP2a family beta-lactam-resistant peptidoglycan
                     transpeptidase"
                     /protein_id="HCD1823178.1"
                     /translation="GEVEMTERPIKIYNSLGVKDINIQDRKIKKVSKNKKRVDAQYKI
                     KTNYGNIDRNVQFNFVKEDGMWKLDWDHSVIIPGMQKDQSIHIENLKSERGKILDRNN
                     VELANTGTAYEIGIVPKNVSKKDYKAIAKELSISEDYIKQQMDQNWVQDDTFVPLKTV
                     KKMDEYLSDFAKKFHLTTNETESRNYPLGKATSHLLGYVGPINSEELKQKEYKGYKDD
                     AVIGKKGLEKLYDKKLQHEDGYRVTIVDDNSNTIAHTLIEKKKKDGKDIQLTIDAKVQ
                     KSIYNNMKNDYGSGTAIHPQTGELLALVSTPSYDVYPFMYGMSNEEYNKLTEDKKEPL
                     LNKFQITTSPGSTQKILTAMIGLNNKTLDDKTSYKIDGKGWQKDKSWGGYNVTRYEVV
                     NGNIDLKQAIESSDNIFFARVALELGSKKFEKGMKKLGVGEDIPSDYPFYNAQISNKN
                     LDNEILLADSGYGQGEILINPVQILSIYSALENNGNINAPHLLKDTKNKVWKKNIISK
                     ENINLLTDGMQQVVNKTHKEDIYRSYANLIGKSGTAELKMKQGETGRQIGWFISYDKD
                     NPNMMMAINVKDVQDKGMASYNAKISGKVYDELYENGNKKYDIDE"
BASE COUNT         1477 a          656 c          738 g         1523 t
ORIGIN      
        1 tattatggaa tagagatgtt ggtaacattt atacaggatc attatactta agtttaattt
       61 cgttattaca gaaccacaca ttccaaccag aagagaaagt atgtctattt agttatggtt
      121 caggagcagt aggagaaatc tttagtggtt caatcgttaa aggatatgac aaagcattag
      181 ataaagagaa acacttaaat atgctagaat ctagagagca attatcagtc gaagaatacg
      241 aaacattctt taacagattt gataatcaag aatttgattt cgaacgtgaa ttgacacaag
      301 atccatattc aaaagtatac ttatacagta tagaagacca tatcagaaca tataagatag
      361 agaaataaac tagtggccga ttgtgcttga tgagcttggg acataaatcc taactcgaaa
      421 taaataagca tatcactaaa ctgatttttt aaagtttaca gtgatatgct tattttttta
      481 tcttacgatt ttgtacgtgc atgcttgcct aggggtatgg ctcgagccat tagtctctcg
      541 cacatactat tccctcaggc gtcagcactt acaaaatcgg ttgtaatttt catttttata
      601 cgcattctta ctgagattat actaataaga ggaatagtaa aagcaattct aagtaaaatt
      661 gcagataaga ggtttgttaa aagcagttct aagtaaaatt gcagataaga ggtttgttaa
      721 aagcagttct cagtaaaatt acagataaga ggtacgttaa aagcagttct aagtaaaatt
      781 gcagataaga ggtttgttaa aagcagttct aagtaaaatt gcagataaga ggtacgttaa
      841 aagcaattcc atgcaaaatt gctgataagg ggtaagttaa aagcagttct cagtaaaatt
      901 gcagataaga ggtacgttaa aagcagttct aggcaaaatt gcagataaga ggtgcgttaa
      961 aagcagttct cagtaaaatt gctgataagg ggtaagttaa aagcaatcct aagtaaaatt
     1021 gcagataagg ggtacagaaa aactagactt gattacaaaa tggagcttgg gacataaatg
     1081 attttttaaa aatgagatga gacgtagatt aactccataa tcaatacgaa tctatcgact
     1141 tctttattta tgatattcat ctctttttaa tggaaataaa agtgcgatta atgtgataat
     1201 acagttacgt taattaaaaa aataaaaatg caaggagagg taatatgcta actgtatatg
     1261 gacatagagg attacctagt aaagctccgg aaaatacaat tgcatcattt aaagctgctt
     1321 cagaagtaga aggtataaac tggttggagt tagatgttgc aattacaaaa gatgaacaac
     1381 tgattatcat tcatgatgat tatttagaac ggactacaaa tatgtccggg gaaataactg
     1441 aattgaatta tgatgaaatt aaagatgctt ctgcaggatc ttggtttggt gaaaaattca
     1501 aagatgaaca tttgccaact ttcgatgatg tagtaaaaat agcaaatgaa tataatatga
     1561 atttaaatgt agaattaaaa ggtattactg gaccgaatgg actagcactt tctaaaagta
     1621 tggttaagca agtggaagaa caattaacaa acttaaatca gaatcaagaa gtgctcattt
     1681 caagctttaa tgttgtgctt gttaaacttg cagaagaaat catgccacaa tataacagag
     1741 cagttatatt ccatacaact tcgtttcgtg aagactggag aacactttta gattactgta
     1801 atgctaaaat agtaaacact gaagatgcca aacttactaa agcaaaagta aaaatggtaa
     1861 aagaagcggg ttatgaattg aacgtatgga ctgtaaacaa accagcacgt gcaaaccaac
     1921 ttgctaattg gggagttgat ggtatcttta cagacaatgc agataaaatg gtgcatttgt
     1981 ctcaatagaa agttagaggt gagtcttacg tttcagtgac ggtagactta cctttaacat
     2041 gttacatact aaaaaattaa tttgaataag aaagagagac atatatgaaa tacgatgatt
     2101 ttatagtagg agaaacattc aaaacaaaaa gccttcatat tacagaagaa gaaattatcc
     2161 aatttgcaac aacttttgat cctcaatata tgcatataga taaagaaaaa gcagaacaaa
     2221 gtagatttaa aggtatcatt gcatctggca tgcatacact ttcaatatca tttaaattat
     2281 gggtagaaga aggtaaatac ggagaagaag ttgtagcagg aacacaaatg aataacgtta
     2341 aatttattaa acctgtatac ccaggtaata cattgtacgt tatcgctgaa attacaaata
     2401 agaaatccat aaaaaaagaa aatggactcg ttacagtgtc actttcaaca tacaatgaaa
     2461 atgaagaaat tgtatttaag ggagaagtaa cagcacttat taataattca taataaaaca
     2521 gtgaagcaac catcgttacg gattgcttca ctgttttgtt attcatctat atcgtatttt
     2581 ttattaccgt tctcatatag ctcatcatac actttacctg agattttggc attgtagcta
     2641 gccattcctt tatcttgtac atctttaaca ttaatagcca tcatcatgtt tggattatct
     2701 ttatcatatg atataaacca cccaatttgt ctgccagttt ctccttgttt cattttgagt
     2761 tctgcagtac cggatttgcc aattaagttt gcataagatc tataaatatc ttctttatgt
     2821 gttttattta cgacttgttg cataccatca gttaatagat tgatattttc tttggaaata
     2881 atatttttct tccaaacttt gtttttcgtg tcttttaata agtgaggtgc gttaatattg
     2941 ccattatttt ctaatgcgct atagattgaa aggatctgta ctgggttaat cagtatttca
     3001 ccttgtccgt aacctgaatc agctaataat atttcattat ctaaattttt gtttgaaatt
     3061 tgagcattat aaaatggata atcacttggt atatcttcac caacacctag ttttttcatg
     3121 cctttttcaa atttcttact gcctaattcg agtgctactc tagcaaagaa aatgttatct
     3181 gatgattcta ttgcttgttt taagtcgata ttaccattta ccacttcata tcttgtaacg
     3241 ttgtaaccac cccaagattt atctttttgc caacctttac catcgatttt ataacttgtt
     3301 ttatcgtcta atgttttgtt atttaaccca atcattgctg ttaatatttt ttgagttgaa
     3361 cctggtgaag ttgtaatctg gaacttgttg agcagaggtt cttttttatc ttcggttaat
     3421 ttattatatt cttcgttact catgccatac ataaatggat agacgtcata tgaaggtgtg
     3481 cttacaagtg ctaataattc acctgtttga gggtggatag cagtacctga gccataatca
     3541 tttttcatgt tgttataaat actcttttga actttagcat caatagttag ttgaatatct
     3601 ttgccatctt ttttcttttt ctctattaat gtatgtgcga ttgtattgct attatcgtca
     3661 acgattgtga cacgatagcc atcttcatgt tggagctttt tatcgtaaag tttttcgagt
     3721 ccctttttac caataactgc atcatcttta tagcctttat attctttttg ttttaattct
     3781 tcagagttaa tgggaccaac ataacctaat agatgtgaag tcgcttttcc tagaggatag
     3841 ttacgacttt ctgtttcatt agttgtaaga tgaaattttt ttgcgaaatc acttaaatat
     3901 tcatccattt ttttaacggt tttaagtgga acgaaggtat catcttgtac ccaattttga
     3961 tccatttgtt gtttgatata gtcttcagaa atacttagtt ctttagcgat tgctttataa
     4021 tcttttttag atacattctt tggaacgatg cctatctcat atgctgttcc tgtattggcc
     4081 aattccacat tgtttcggtc taaaatttta ccacgttctg attttaaatt ttcaatatgt
     4141 atgctttggt ctttctgcat tcctggaata atgacgctat gatcccaatc taacttccac
     4201 ataccatctt ctttaacaaa attaaattga acgttgcgat caatgttacc gtagtttgtt
     4261 ttaattttat attgagcatc tactcgtttt ttatttttag atactttttt tattttacga
     4321 tcctgaatgt ttatatcttt aacgcctaaa ctattatata tttttatcgg acgttcagtc
     4381 atttctactt cacc
//
LOCUS       DAHHSC010000190         4392 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.191, whole genome shotgun sequence.
ACCESSION   DAHHSC010000190 DAHHSC010000000
VERSION     DAHHSC010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 4392)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4392)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4392
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.191"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            15..1190
                     /gene="rpsA"
                     /locus_tag="NBI57_002250"
     CDS             15..1190
                     /gene="rpsA"
                     /locus_tag="NBI57_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HCD1823179.1"
                     /translation="MTEEFNESMINDIKEGDKVTGEVQQVEDKQVVVHINGGKFNGII
                     PISQLSTHHIDSPSEVVKEGDEVEAYVTKVEFDEENETGAYILSRRQLETEKSYSYLQ
                     EKLDNNEIIEAKVTEVVKGGLVVDVGQRGFVPASLISTDFIEDFSVFDGQTIRIKVEE
                     LDPENNRVILSRKAVEQEENDAKKDQLLQSLNEGDVIHGKVARLTQFGAFIDIGGVDG
                     LVHVSELSHEHVQTPEEVVSIGQDVKVKIKSIDRDTERISLSIKDTLPTPFENIKGQF
                     HENDVIEGVVARLANFGAFVEIAPGVQGLVHISEIAHKHIGTPGEVLEPGQQVNVKIL
                     GIDEENERVSLSIKATLPNEDVVESDPSTTKAYLENEEEDNPTIGDMIGDKLKNLKL"
     gene            1412..2722
                     /gene="der"
                     /locus_tag="NBI57_002251"
     CDS             1412..2722
                     /gene="der"
                     /locus_tag="NBI57_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019167598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HCD1823180.1"
                     /translation="MTKPIVAIVGRPNVGKSTIFNRIVGERVSIVEDTPGVTRDRIYS
                     SGEWLTHDFNIIDTGGIEIGDAPFQTQIRAQAEIAIDEADVIIFMVNVREGLTQSDEM
                     VAQILYKSKKPVVLAVNKVDNMEMRTDVYDFYSLGFGEPYPISGSHGLGLGDLLDAVV
                     SHFGEEEEDPYDEDTIRLSIIGRPNVGKSSLVNAILGEDRVIVSNVAGTTRDAIDTEY
                     SYDGQDYVLIDTAGMRKKGKVYESTEKYSVLRALKAIERSNVVLVVIDAEQGIIEQDK
                     RVAGYAHEQGKAVVIVVNKWDTVEKDSKTMKKFEDEVRKEFQFLDYAQIAFVSAKERT
                     RLRTLFPYINEASENHKKRVQSSTLNEVVTDAISMNPTPTDKGRRLNVFYATQVAIEP
                     PTFVVFVNDVELMHFSYKRYLENQIRAAFGFEGTPIHIIARKRN"
     gene            2739..3737
                     /locus_tag="NBI57_002252"
     CDS             2739..3737
                     /locus_tag="NBI57_002252"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HCD1823181.1"
                     /translation="MTKITVFGMGSFGTALANVLAENGHDVLMWGKNQDAVDELNTCH
                     TNKKYLKYAKLDVNIIATSDMTKAIQFADIYLMALPTKAMREVATQINDKLTSKKTFI
                     HVAKGIENGTFKRVSEMIEDSISPEYNAGIGVLSGPSHAEEVVVKQPTTVAASSKDKS
                     VSKLTQDLFMNDYLRVYTNDDLIGVELGGALKNIIAVASGIVAGIGYGDNAKAALMTR
                     GLAEISRLGEKLGADPMTFLGLGGIGDLIVTCISTHSRNFTLGYKLGQGESMDQALSE
                     MNMVVEGIYTTKSVYHLAKEKNVDMPITNALYRVLFENISVKECVKDLMERDKKSE"
     gene            3908..4180
                     /locus_tag="NBI57_002253"
     CDS             3908..4180
                     /locus_tag="NBI57_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015900354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HCD1823182.1"
                     /translation="MNKTDLINAVAEQADLTKKEAGSAVDAVFESIQNSLAKGEKVQL
                     IGFGNFEVRERAARKGRNPQTGKEIDIPASKVPAFKAGKALKDAVK"
BASE COUNT         1577 a          594 c          881 g         1340 t
ORIGIN      
        1 aggaggcaaa caagatgact gaagaattca atgaatcaat gattaacgat attaaagaag
       61 gtgacaaagt cactggcgag gtacaacaag ttgaagacaa gcaagttgtt gttcatatca
      121 acggtggtaa atttaatggg attattccta ttagtcaact atctacgcat catattgata
      181 gcccaagtga agttgtaaaa gagggcgacg aagttgaagc atatgtcact aaagttgagt
      241 ttgatgaaga aaatgaaact ggagcttaca tcttatctag aagacaactt gaaactgaga
      301 agtcttatag ttatttacaa gaaaaattag ataataatga aatcatcgaa gcgaaagtaa
      361 cagaagtagt taaaggtggt ttggttgttg atgtaggaca aagaggtttt gttccggctt
      421 cactaatttc aacagacttc attgaggatt tctctgtgtt tgatggacaa acaattcgta
      481 ttaaagttga agaattggat cctgaaaata atagagtcat tttaagccgt aaagcagttg
      541 aacaagaaga aaacgatgct aaaaaagatc aattattaca atctttaaat gaaggcgatg
      601 ttattcatgg taaagtagcg cgtttaactc aatttggtgc atttatagac attggtggtg
      661 ttgatggttt agtgcatgta tctgaacttt ctcacgaaca tgttcaaaca ccagaagaag
      721 tagtttcaat tggtcaagat gttaaagtta aaattaaatc tattgataga gatacagaac
      781 gtatttcatt atcaatcaaa gatacgttac caacaccttt cgaaaatatt aaaggtcaat
      841 tccacgaaaa tgatgtcatt gaaggtgtcg tagcaagatt ggcaaacttt ggtgcatttg
      901 ttgaaattgc accaggtgta caaggacttg tacatatttc tgaaattgca cacaaacaca
      961 ttggtacgcc aggtgaagtg ttagaacctg gtcaacaagt aaatgttaaa atattaggta
     1021 ttgatgaaga gaatgaaaga gtatcactat ctattaaagc aacattacca aacgaagatg
     1081 ttgttgaaag tgatccttct acgactaagg cgtacttaga aaacgaagaa gaagataatc
     1141 caacaattgg cgatatgatt ggtgataaac ttaaaaatct taaactataa tttaatattt
     1201 aatagtcaac tccacatgtt tatgattgca tgtggagtat ttttatgtaa caaaatatac
     1261 tcggaatgat aacgtggaca aatttaacta agtgtttaaa aagataaagt tttaagtgct
     1321 gatttttatc attacagtaa taaactcatt ttgaatacac agtctcatgt gatattatta
     1381 aaaagatata agaaagagag gaagttagct tatgactaaa cctatagtag ctattgtagg
     1441 taggcctaat gtaggtaaat ctacaatttt taatagaata gttggagaac gtgtttcgat
     1501 tgtggaagac acgccaggtg taacacgaga tcgtatttat tcttcaggtg aatggttaac
     1561 acatgatttc aatattattg atacaggtgg tattgaaatt ggtgatgcac cattccaaac
     1621 acaaattaga gcgcaggcag aaatcgccat agatgaagcg gatgttatta tttttatggt
     1681 taacgtgcgt gaaggattga cacaaagcga tgaaatggtc gctcaaattt tatacaaatc
     1741 taaaaaaccg gtcgtattag cggttaacaa agtagataat atggaaatgc gtacagacgt
     1801 gtatgatttc tattcattag gatttggtga accgtatccg atatcagggt cacatggttt
     1861 aggtcttggt gacttgttag atgcagttgt ttctcatttt ggtgaagagg aagaagatcc
     1921 ttatgatgaa gatacaattc gactatccat tattggacga ccaaacgtag gtaaatcaag
     1981 tttagtaaat gctattttag gtgaagatcg cgttatcgtt tctaatgttg cagggacaac
     2041 gagagacgct attgatacag agtatagtta tgatggacaa gattatgttt taatcgatac
     2101 tgctggtatg cgtaaaaaag gaaaagtata tgaatcaact gagaaatatt cagtactaag
     2161 agctttaaaa gcgattgaac gttcaaatgt tgttttagtg gtcattgatg cagaacaagg
     2221 catcattgaa caagataaac gtgttgcagg atatgcacat gaacaaggta aagcagtcgt
     2281 gattgtcgta aataaatggg atactgtgga aaaagatagt aaaacgatga agaaatttga
     2341 agatgaagta cgtaaagaat tccaattttt agattatgca caaattgctt ttgtgtctgc
     2401 taaagaacgc acaagattac gtacattatt cccttacatt aatgaagcaa gtgaaaacca
     2461 taaaaaacgt gttcaaagtt caactttaaa tgaagttgtt actgatgcaa tttccatgaa
     2521 ccctacacca acagacaaag gtagacgttt gaatgtcttt tatgcaacac aagttgctat
     2581 agaaccaccg acatttgttg tatttgttaa tgatgtagaa ttaatgcatt tttcttataa
     2641 acgctattta gagaatcaaa tccgtgccgc ttttggtttt gaaggtacac caattcatat
     2701 tatagctcga aagagaaatt aacgattggg ggataacaat gactaaaatt accgtttttg
     2761 gtatgggaag ttttgggaca gcccttgcca atgttcttgc agaaaatgga catgatgttt
     2821 tgatgtgggg taaaaatcaa gatgctgttg atgaattaaa tacatgtcat acaaataaaa
     2881 agtatttaaa atacgcgaaa ttagatgtta acatcatcgc tacttcagat atgaccaaag
     2941 caattcaatt tgcagatatt tacttaatgg ctttacctac taaagcaatg cgagaagttg
     3001 ctactcaaat taatgataag ctgacctcta aaaagacttt tatacatgtt gctaaaggta
     3061 ttgaaaatgg gacgtttaaa cgtgtgtcag aaatgattga agattctatt tcacccgaat
     3121 ataatgcagg tattggcgtg ttgtcagggc caagtcatgc ggaagaagtt gtagtcaagc
     3181 aaccaactac agttgctgca tcatcaaaag ataaaagtgt aagtaaatta acgcaagatt
     3241 tatttatgaa tgattatttg cgtgtgtaca cgaatgatga cttgattggt gttgaacttg
     3301 gtggtgcatt gaaaaatatc atcgcagtag caagtggtat cgtagctgga attggctacg
     3361 gtgataatgc aaaagctgcg ttaatgactc gtggtttagc ggaaattagt agattaggtg
     3421 aaaagttagg tgccgatcct atgacatttc taggtttagg tggtatcggt gacttaatcg
     3481 ttacttgcat atcaacacat tctcgaaatt tcacattagg atataaactt ggacaaggtg
     3541 aatcaatgga tcaagcatta tctgaaatga atatggttgt tgaaggtatt tatacaacta
     3601 aatcagttta tcatttagct aaagaaaaaa atgtggatat gccaattaca aatgcattat
     3661 atagagtatt atttgaaaat atctcagtaa aagaatgcgt aaaagattta atggagcgcg
     3721 ataaaaaatc tgaataaaat gtgtattatt acacatatac ggctaataat tacgtaaaat
     3781 gaatagaaat ggcttaaatt caacgttttt gccacataaa tgattgcgat ttatgtaatg
     3841 cttgtgttaa agttttacca taatgatata aacatatcat tatcaaacct caggaggtga
     3901 atgtctaatg aacaaaacag atttaatcaa tgcagttgca gagcaagctg atttaactaa
     3961 aaaagaagct ggttcagcag tagatgctgt attcgaatca atccaaaact cacttgctaa
     4021 aggtgaaaaa gtacaattaa ttggtttcgg taactttgag gtacgtgaac gtgctgcacg
     4081 taaaggtcgt aaccctcaaa ctggtaaaga aattgatatc ccagcaagta aagttccagc
     4141 attcaaagct ggtaaagcat taaaagatgc tgtaaaataa ttttacttaa aaagccctga
     4201 ataagggctt tttattttgc ttttaatact tacaacctgt acataatttg taatgttctt
     4261 ctaagttttt aatctttgga acattcattg tttaagcgtt caaaatatta catgacataa
     4321 tagttttgat aaaaatagca actacaaaat atacatatga ataatgagat taattgttag
     4381 cgttgaattt ac
//
LOCUS       DAHHSC010000191         4371 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.192, whole genome shotgun sequence.
ACCESSION   DAHHSC010000191 DAHHSC010000000
VERSION     DAHHSC010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 4371)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4371)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4371
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.192"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            6..89
                     /locus_tag="NBI57_002254"
     CDS             6..89
                     /locus_tag="NBI57_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1823183.1"
                     /translation="MILTCYNTFNTIEFKSKISGIDEARSK"
     gene            115..2250
                     /locus_tag="NBI57_002255"
     CDS             115..2250
                     /locus_tag="NBI57_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HCD1823184.1"
                     /translation="MKSLILAEKPSVARDIADALQINQKRNGYFENNQYIVTWALGHL
                     VTNATPEQYDKNLKEWRLEDLPIIPKYMKTVVIGKTSKQFKTVKALILDNKVKDIIIA
                     TDAGREGELVARLILDKVGNKKPIRRLWISSVTKKAIQQGFKNLKDGRQYNDLYYAAL
                     ARSEADWIVGINATRALTTKYDAQLSLGRVQTPTIQLVNTRQQEINQFKPQQYFTLSL
                     TVKGFDFQLESNQRYTNKETLEQMVNNLKNVDGKIKSVATKHKKSYPQSLYNLTDLQQ
                     DMYRRYKIGPKETLNTLQSLYERHKVVTYPRTDSNYLTTDMVDTMKERIQATMATTYK
                     DQARPLMSKTFSSKMSIFNNQKVSDHHAIIPTEVRPVMSDLSNRELKLYDMIVERFLE
                     ALMPPHEYDAITVTLEVAGHTFVLKENVTTVLGFKSIRQGESITEMQQPFSEGDEVKI
                     SKTNIREHETTPPEYFNEGSLLKAMENPQNFIQLKDKKYAQTLKQTGGIGTVATRADI
                     IDKLFNMNAIESRDGKIKVTSKGKQILELAPEELTSPLLTAQWEEKLLLIERGKYQAK
                     TFINEMKDFTKDVVNGIKNSDRKYKHDNLTTTECPTCGKFMIKVKTKNGQMLVCQDPS
                     CKTKKNVQRKTNARCPNCKKKLTLFGKGKEAVYRCVCGHSETQAHMDQRMKSKSSGKV
                     SRKEMKKYMNKNEGLDNNPFKDALKNLNL"
     gene            2363..3697
                     /locus_tag="NBI57_002256"
     CDS             2363..3697
                     /locus_tag="NBI57_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000800694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HCD1823185.1"
                     /translation="MKNYFQFDKYGTNFKREILGGITTFLSMAYILAVNPQVLSLAGV
                     KGVSEDMKMDQGAIFVATALAAFVGSLFMGLIAKYPIALAPGMGLNAFFAFTVVLTMG
                     IPWQVGLTGVLFSGIFFAILTVTGFREVIINAIPYQMKMAVSAGIGLFITFVGLQSSG
                     IIVKNESTLVTLGHLTDPPVLLAIFGIVITVILYAIKLPGSIFIGMIITAIVGMFTGL
                     IQMPSGIVGKIPSIEPTFGAAFEAFKDPSQLLTIQFLIVILTFLFIDFFDTAGTLVAV
                     ATQAGIMKDNKLPRAGRALFSDSLATIVGSVFGTTTTTSYIESTSGVAVGARTGFASI
                     VTGCCFLLALFFSPLIAVVTSAVTTPALVVVGVLMAANFAEINWKSFEVAVPAFITII
                     MMPLSYSIATGIACGFIFYPITMLISKKHKEVHPIMYVLMVLFILYFIFVHG"
     gene            complement(3888..4277)
                     /locus_tag="NBI57_002257"
     CDS             complement(3888..4277)
                     /locus_tag="NBI57_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1823186.1"
                     /translation="MKNGPKLSLALIGIFLILCEFFYGIPFLGATFILSFGWQPLLFN
                     ALLYLILTIILLVNRQNAIRPIAIIPIFGIVGSFLAIIPFLGILIHWILFFLMILFVL
                     VVLSAPTYIPNKNARVIYTQYKDGHRE"
BASE COUNT         1609 a          638 c          789 g         1335 t
ORIGIN      
        1 taatagtgat attaacatgt tacaatacat ttaacaccat tgaatttaaa tcaaagatta
       61 gtggaataga tgaagcacgt tcgaaataaa agaacgtatg agaaaggata atttatgaaa
      121 tctttaatat tagctgaaaa accatcagtt gcaagagata ttgctgatgc tttacaaata
      181 aatcagaagc gtaatggtta ctttgaaaat aaccaatata ttgtcacgtg ggcgttaggt
      241 catctagtga caaatgcgac acctgaacaa tacgataaaa atttaaagga atggcgatta
      301 gaagaccttc caattatacc taaatatatg aaaactgttg ttattggtaa aacaagcaaa
      361 caatttaaaa cagtaaaagc gttaatttta gataataaag tgaaagatat tattattgca
      421 acagatgctg gacgagaagg tgaactagtt gcaagattga ttttggataa agttggtaac
      481 aaaaagccaa tccgtcgatt atggattagc tcagttacta aaaaagctat tcaacaaggt
      541 tttaaaaatt taaaagacgg tcgtcaatat aacgatttgt attatgcagc gttagcgaga
      601 agcgaggcag attggattgt tgggattaat gcaacgcgtg cactaacaac aaagtatgat
      661 gcacagctat ccctgggacg tgttcagaca ccaacgattc aattagtaaa tacacgacaa
      721 caagagatta atcagttcaa accacaacaa tactttacat tatcattaac ggtaaaaggg
      781 tttgattttc agctagaatc aaatcagcga tataccaata aagaaacttt agaacagatg
      841 gttaataatt tgaaaaatgt cgatggtaag attaaatctg ttgctactaa acataagaag
      901 tcgtatccgc aatcactgta caatttaaca gatttacaac aagatatgta tagacgttat
      961 aaaattggac ctaaagaaac attgaataca cttcaaagct tatatgagag acataaagtc
     1021 gtaacctatc caagaacaga ttcaaactat ttaacaactg atatggtaga tactatgaaa
     1081 gaacgtattc aggcgacgat ggcaacaaca tataaagacc aagcacgccc attaatgtct
     1141 aaaacatttt catcaaaaat gtcgatattt aataatcaaa aagtatctga tcaccatgca
     1201 attattccta cagaagtgag acctgtcatg tcagacttaa gtaatagaga attaaagtta
     1261 tacgatatga ttgtcgagcg ttttttagaa gctttaatgc ctccgcacga gtatgacgcg
     1321 ataactgtaa ctttagaggt tgcagggcac acatttgttt tgaaagagaa tgtaacaact
     1381 gttttaggtt ttaaatctat tagacaaggt gaatctatta cagagatgca acagcctttt
     1441 tcagaaggcg atgaagtgaa gatttcaaaa acaaacatta gagaacatga aacaacacct
     1501 ccagaatatt ttaatgaagg ttcgttatta aaagcgatgg agaaccctca gaactttatt
     1561 caattgaagg ataaaaaata tgcgcaaact ttaaaacaaa caggtggtat cggcacagtt
     1621 gcaacaaggg ccgacattat cgataaatta tttaatatga atgccattga atcaagagac
     1681 ggtaaaatta aagtaacgtc aaaaggtaaa caaatattag aattagcacc agaagaatta
     1741 acgtcgccac ttttaactgc acaatgggaa gaaaaattac ttttaattga acgtggtaaa
     1801 tatcaggcga aaacatttat taatgaaatg aaagatttta cgaaagatgt tgtaaatggg
     1861 attaaaaata gtgatcgtaa atataaacac gataatttaa caaccacaga atgcccaacg
     1921 tgtggtaaat tcatgattaa agttaaaact aaaaatggtc agatgcttgt gtgccaagat
     1981 ccatcttgta agacgaaaaa gaatgtacag cgcaaaacaa atgcaagatg tccaaactgt
     2041 aaaaagaaat taacgttgtt tggtaaaggg aaagaagcgg tatatcgttg tgtttgtgga
     2101 cattctgaaa cgcaagcaca tatggatcag cgtatgaagt ctaaatcctc tggtaaagta
     2161 tctcgtaaag aaatgaaaaa gtatatgaat aaaaatgaag gtttagacaa taatccgttt
     2221 aaagatgcat taaagaactt gaatttatag ataaaatcga acaaagttga atcagaaaaa
     2281 cgaaaagttc gcttttggta ttgtttttta ttaagaatga tattaaacta ttaaggtatt
     2341 ttaaaaaaag gagcatccat tcgtgaaaaa ctatttccag ttcgataaat atggaacaaa
     2401 ctttaaaaga gaaatcttag gcggtatcac aactttctta tctatggcct atattttagc
     2461 agttaacccg caagttttaa gtttagcagg tgttaaaggc gtatcagaag atatgaaaat
     2521 ggaccaaggt gccatttttg tagcgactgc attagcagca tttgtaggct cgctattcat
     2581 gggactaata gctaaatatc caatcgcatt agcaccaggt atgggattaa atgcattctt
     2641 tgcatttact gttgtgttaa ccatgggtat tccttggcaa gttggtttaa caggtgtttt
     2701 attctcaggt atattctttg cgatattaac cgtcacaggg tttagggaag ttattattaa
     2761 tgcaattcct tatcaaatga agatggctgt ttcagcaggt ataggattat tcattacatt
     2821 tgtaggttta caaagttcgg gtattatagt taaaaatgaa tctacattag taacattggg
     2881 acatttaact gatccaccag tacttttggc gatattcggt atcgttatta cagttatttt
     2941 atatgcaatt aaattgccgg gttctatctt tatcggtatg atcatcacag caatcgttgg
     3001 tatgttcact ggattgattc aaatgccatc tggtattgta ggtaagattc caagtattga
     3061 accaacgttc ggtgctgcat ttgaagcgtt taaagaccca agtcaattat taacgataca
     3121 gttcttaatt gttattttaa cgttcttatt tattgatttc tttgatacag ctggtacatt
     3181 agtagctgtt gctactcaag caggtattat gaaagataat aaattaccaa gagctggtag
     3241 agcattgttc tcagattctt tagctacgat tgtaggatca gtttttggaa caactacaac
     3301 aacttcttat atcgaatcaa cttcaggtgt tgctgtaggt gctagaacag gatttgctag
     3361 tatcgtaaca ggttgttgtt tcctattagc gctgttcttt agtccgttaa tcgctgttgt
     3421 aacaagtgca gttacgacac cagctttagt ggtggtaggt gtattaatgg ctgcaaactt
     3481 tgctgagata aactggaaaa gttttgaagt tgcagtgcca gcattcatta caatcattat
     3541 gatgccttta tcatactcaa ttgcaactgg tattgcgtgt ggatttattt tctatccaat
     3601 tacaatgctt atctctaaaa aacataaaga agtacatccg ataatgtatg tattaatggt
     3661 actattcatt ctgtacttca tatttgttca tggttaaatc cagacacaaa aaacgactgc
     3721 caatgattgg tagtcgtttt ttctttgaag taaggataaa ataagaagcg aaataacata
     3781 aagcaatatt atgaatgtct ttaaatatcc gatataaata tacttctttt aaaattgttg
     3841 gacgtgtttc ttctatatta tatacctaat taaaaaggtt ttaagaatta ttctctatgt
     3901 ccatctttgt attgtgtgta aatgacacga gcatttttat taggtatata ggttggcgct
     3961 gataatacaa ctaggacaaa tagaatcatt aggaaaaata aaatccaatg aatcaaaatt
     4021 cctaagaagg ggatgatagc taaaaaacta cctactatac caaatattgg aataatcgct
     4081 ataggtctaa tcgcattttg tctattcact aataatataa tagtaagaat tagataaagt
     4141 aatgcattaa agagtaaagg ctgccatcca aaacttaaaa taaacgttgc acctaaaaaa
     4201 ggaatgccat agaaaaactc acaaagtatt aaaaagatac ctataagtgc taaagataat
     4261 tttggtccat ttttcatatt cattcaccac cgttcttatg actaattata tagaataatt
     4321 ttcaatgata ataataaaat gattcaaggt acgaatttac aataaagaag g
//
LOCUS       DAHHSC010000192         4332 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.193, whole genome shotgun sequence.
ACCESSION   DAHHSC010000192 DAHHSC010000000
VERSION     DAHHSC010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 4332)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4332)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4332
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.193"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            351..1310
                     /locus_tag="NBI57_002258"
     CDS             351..1310
                     /locus_tag="NBI57_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001081330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCD1823187.1"
                     /translation="MNRNIVKLVVFMLILVVAVAGCGQKDTEEKTEMTTIKDELGTEK
                     IKKNPKRVVVLEYSFADYLAALDMKPVGIADDGSTKNITKSVRDKIGAYESVGSRPQP
                     NMEVISKLKPDLIIADVSRHKKIKSELSKIAPTIMLVSGTGDYNANIEAFKTVAKAVG
                     KEKEGEKRLEKHDKILAEIRKKIEQSTLKSAFAFGISRAGMFINNEDTFMGQFLIKMG
                     IQPEVTKDKTTHVGERKGGPYIYLNNEELANINPKVMILATDGKTDKNRTKFIDPAVW
                     KSLKAVKDNKVYDVDRNKWLKSRGIIASESMAEDLEKIAEKAK"
     gene            complement(1356..1487)
                     /locus_tag="NBI57_002259"
     CDS             complement(1356..1487)
                     /locus_tag="NBI57_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1823188.1"
                     /translation="MRYVTMRQFIKRTVKTILVGYVIKFIRNKLSGKSSHPTDNKHN"
     gene            complement(1551..1766)
                     /locus_tag="NBI57_002260"
     CDS             complement(1551..1766)
                     /locus_tag="NBI57_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000867358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TM2 domain-containing protein"
                     /protein_id="HCD1823189.1"
                     /translation="MKVNKAIYVIVALFLGGLGIHKFYADKFGQGLFHLLFFWTGIPT
                     IISIIHAIFVIFTKKADKDGYIIFPKN"
     gene            1931..2482
                     /locus_tag="NBI57_002261"
     CDS             1931..2482
                     /locus_tag="NBI57_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCD1823190.1"
                     /translation="MIRQARPEDRFDIAKLVYMVWDDMELELVKHLPKDTVLDAIEKS
                     CVDATYRTFYQHILVYEVENKVAGCIISYSGENELKYEKAWELLDLPEEIKQYGTPLP
                     VKEAKDDEYYIETIATFAAYRGRGIATKLLTSLLESNTHVKWSLNCDINNEAALKLYK
                     KVGFISDGQIELYKHMYHHLIVK"
     gene            complement(2534..3472)
                     /locus_tag="NBI57_002262"
     CDS             complement(2534..3472)
                     /locus_tag="NBI57_002262"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HCD1823191.1"
                     /translation="MSNQYQQYSTVKKYWHLMRPHTLTASVVPVLVGTAASKIYFLGS
                     EDHIKISLFIAMLLACLLIQAATNMFNEYYDYKKGLDDHESVGIGGAIVRNGMSPELV
                     LRLAIAFYILAAILGLFLAANSSFWLLPVGLVCMAVGYLYTGGPFPISWTPFGELFSG
                     VFMGMFIIVIAFFIQTGNIQSYVIWLSVPIVITIGLINMANNIRDRVKDKASGRKTLP
                     ILLGKNASLTFMAIMYFIAYAFIVLTIIIKPGGSLFYLLALLSFPMPVKVIRRFKKND
                     TPPTMMPAMAAAGKTNTFFGLLYALGIYISALFAGI"
     gene            3623..3727
                     /locus_tag="NBI57_002263"
     CDS             3623..3727
                     /locus_tag="NBI57_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1823192.1"
                     /translation="MKKDEERSEMDGYGRIRGRYCQRDIWQLKGMGFS"
     gene            3804..>4332
                     /locus_tag="NBI57_002264"
     CDS             3804..>4332
                     /locus_tag="NBI57_002264"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase"
                     /protein_id="HCD1823193.1"
                     /translation="MRLNDNRTSYFGYKAIQLFKNNSKNKQSIFKDWEKLKHNITFIH
                     PQSEKHHLRVVGGFQFSSHKSDDEWREFGLNHFVLPEVLISTDNNGTFLTYTVKRESF
                     TVEALNDLMDLFNNISDIDVDEQIGEITRNEDIYKDDWRQLVVEAIESINNEEKIVLA
                     RRRLIKFDKDISIPYI"
BASE COUNT         1663 a          584 c          787 g         1298 t
ORIGIN      
        1 aagatgttta agtcatcagt tactttttgt atttataaat aaagattagt acagcatagt
       61 gcaaagatta tagggaaatg aattaactat tgcatcgcta tttatgaatc gtaaacacta
      121 ttatgaaatt gcgagagtag tatggataat tagaatgatg gtttgagata aatgagtatt
      181 attaaccagt gcgtcatctt caaaattaga ggccatttgc tgtttataat aagaagattt
      241 gataataaac tatgataaga aaaaataata gcataccaaa tatatcataa acaaattaaa
      301 tgataaaata ataatgcagt tttctattaa agttgtttgg aggtaaatat gtgaatagga
      361 atatcgttaa attagttgtg tttatgctaa tcttagttgt agcagtagcg ggttgtggtc
      421 aaaaagatac tgaagagaaa actgaaatga cgacaataaa agatgaatta ggaactgaaa
      481 aaattaagaa aaatcctaaa cgtgttgttg tattagaata tagttttgct gattatttag
      541 cagcattaga tatgaaacct gttggtattg cagatgatgg cagcactaaa aatataacaa
      601 agtcagtaag agataagatt ggggcatatg aatcggttgg atctagaccg caaccgaata
      661 tggaagtgat aagtaaatta aaaccggatt tgatcattgc agatgttagc agacataaga
      721 aaatcaaatc agaattgagc aaaattgctc cgacaatcat gttagttagc ggtacgggag
      781 attataatgc aaatattgaa gcatttaaaa cagtcgctaa agcagtaggc aaagagaaag
      841 aaggcgagaa gcgtctggaa aagcatgata aaatattagc ggagattaga aagaaaattg
      901 aacagagtac gttaaaatct gcatttgcat tcggtatctc aagagcaggt atgtttatta
      961 ataatgaaga tacatttatg ggacaattct taattaaaat gggtattcaa cctgaagtca
     1021 caaaagacaa aactacgcat gttggtgaac gcaagggtgg tccttatata tatttaaata
     1081 atgaagaact tgccaatatc aatccaaaag ttatgatttt agccactgac ggaaaaacgg
     1141 acaaaaatag aacgaaattc attgatcctg cagtttggaa atcattaaaa gctgtgaaag
     1201 ataacaaagt ttatgacgtt gaccgaaata agtggttgaa atcaaggggg attatcgcaa
     1261 gtgaaagtat ggcagaagat ttagaaaaaa ttgcagaaaa agcaaaataa aaatacagcg
     1321 ctactcgtaa atcatataag agtggcgctg tatttttaat tatgtttatt atctgtcgga
     1381 tgtgatgatt tacctgaaag tttatttcga ataaatttaa ttacataacc gacaaggatt
     1441 gttttaacag ttcttttaat gaattggcgc atcgttacat acctcatttc tctatatctt
     1501 acgaactata tacccattca tataagcttt ttaaacgtca ttgtcacaat ttaattttta
     1561 gggaatataa tataaccatc tttatctgct tttttagtaa aaatgacaaa aattgcatgt
     1621 attattgaga tgatggtagg gatacctgtc cagaaaaata ataagtgaaa aagaccttgt
     1681 ccaaatttat cagcataaaa tttatgaata cctaaacctc caagaaataa tgcaacaata
     1741 acataaatgg ctttattgac tttcatttgt aaatcctcct taactataat tctacttaaa
     1801 ttcgttgtga aaaccaatat ttctaacttt agaattttca aactttctaa aattataagt
     1861 atatcttttt aaaataagct agaatttcta tataataaat gttaataacg taaaagggaa
     1921 tgatgacata gtgatacgtc aagcacgtcc agaggaccga tttgatattg cgaagttagt
     1981 ttatatggtt tgggatgata tggaattaga attggtaaag catctaccta aagacacggt
     2041 attagatgca attgaaaaaa gctgtgttga tgcaacatat cgaacttttt atcagcatat
     2101 tttagtttat gaagtagaaa ataaagtagc aggttgtatt attagctata gtggtgaaaa
     2161 tgaattgaaa tacgaaaaag catgggaact acttgacttg ccagaagaaa taaaacaata
     2221 tggcacgcca ttacctgtaa aagaagctaa agacgatgag tattatatag aaacaattgc
     2281 gacatttgca gcatatagag gtagaggcat cgcgacaaag ttattaacgt cattacttga
     2341 atcaaataca catgttaaat ggagtttgaa ttgcgatatt aataatgaag cagcattaaa
     2401 gttatataaa aaagtaggct ttatatctga tggacagatt gaattataca agcacatgta
     2461 tcatcattta attgttaaat aaaaaaactc gacagttcga tgtaagtcga ttgccgagta
     2521 gtatcatttt ctattaaatg cctgcaaata atgcactaat ataaatacct aatgcatata
     2581 ataaaccgaa aaatgtattt gttttaccag cagcagccat tgctggcatc attgtaggcg
     2641 gtgtatcatt cttcttgaaa cgtctgataa ctttaacagg cattgggaat gataacaacg
     2701 caagtaagta aaataatgag ccaccaggtt taataatgat cgtaagtaca ataaaggcat
     2761 aagcgataaa gtacatgatt gccataaatg ttaaagaagc atttttacct aatagaatgg
     2821 gtaaagtttt gcgaccactt gctttatctt tgacacggtc gcgaatattg ttagccatat
     2881 taattaaacc gatagtgatt actataggta cacttaacca aattacataa ctttgaatat
     2941 tgccagtttg aataaagaat gcaataacga taataaacat acccataaat acgcctgaga
     3001 ataattcacc gaaaggcgtc catgaaatag ggaaagggcc acctgtatat aggtaaccaa
     3061 cagccataca tactaatcca actggtaata accaaaatga agagttagca gctaaaaaca
     3121 aacctaatat tgctgctaag atgtaaaatg caatggctaa tcgtagcaca agctctgggc
     3181 tcataccgtt gcgaacaatg gcaccaccaa tgcctacaga ttcatgatca tcaaggcctt
     3241 ttttataatc atagtattca ttaaacatat tagttgctgc ttgaataagt aagcatgcta
     3301 gtaacatggc aatgaatagg ctgattttaa tatgatcttc gctaccaaga aaatatattt
     3361 tagatgctgc tgtaccaact aaaacgggta ctacggaagc agttaatgta tgaggacgca
     3421 ttaaatgcca atatttctta actgtagaat attgctgata ttgattactc attgaataca
     3481 aaacctcttt aaatcatatt ttttataaca aagatatttt aatagaaagt agcataaagg
     3541 tcaattgacg tgaaaaattg caagataagt ttaagcaatt cttgtaacac aattatccaa
     3601 aaatgataca atataataat gagtgaaaaa agacgaagaa agaagtgaaa tggatggcta
     3661 cgggcgtatt agaggacgat attgtcaaag agatatatgg cagctcaaag gaatgggttt
     3721 cagttgaagt gaaattatca cagtcactag acccgagcac attatttcat ctcactgaca
     3781 atgaggcagg agatcgcttt tatatgcgtt tgaatgataa tcgaacgtca tattttggct
     3841 acaaagcaat tcaattattc aaaaataatt ctaaaaataa acaatctatt tttaaagact
     3901 gggaaaaatt aaaacataac atcacattta tacatccgca atctgagaaa catcatcttc
     3961 gagttgttgg agggtttcaa ttttcaagtc ataaatcaga tgatgaatgg cgagagtttg
     4021 gactaaatca ttttgtatta cctgaagttt taatttcaac tgataataat gggacatttt
     4081 taacttatac agttaaaagg gaaagtttta ctgttgaggc attgaacgat ttaatggatt
     4141 tgttcaacaa tatatcggat atagatgtgg acgagcaaat tggggaaatt actagaaatg
     4201 aagatattta taaagatgac tggcgtcaac ttgtagtaga agctatagaa tctattaata
     4261 atgaagaaaa aattgtacta gcacgtagac ggttaataaa gttcgataaa gatatcagta
     4321 ttccatatat tc
//
LOCUS       DAHHSC010000193         4313 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.194, whole genome shotgun sequence.
ACCESSION   DAHHSC010000193 DAHHSC010000000
VERSION     DAHHSC010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 4313)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4313)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4313
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.194"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            complement(61..696)
                     /locus_tag="NBI57_002265"
     CDS             complement(61..696)
                     /locus_tag="NBI57_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 domain-containing protein"
                     /protein_id="HCD1823194.1"
                     /translation="MLLCESKVINKNPKYRIIKYESEYLMIDLASNWIVFFFPFINWL
                     IPKTYVKITKNDYEKLNIVKPVKNKSIGWTIFAGIVLLGGTVRRNTYLFDFQLEKLIV
                     WSSCFIGFLGVIFFYCYLNKKLTLNVYKENKNNELKLRLLPSFKNMCFTIFYYLFTGF
                     MSYGAFYLLVFENVQNLILYISWLFMTMLFMLMNMHSIIDKKVHIFLKSNK"
     gene            complement(697..2091)
                     /locus_tag="NBI57_002266"
     CDS             complement(697..2091)
                     /locus_tag="NBI57_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCD1823195.1"
                     /translation="MSIDMYLDRSRNQASSVGNLSQTMNSNYDALEKAITQFINDDAL
                     KGKAYTSAKQFFSTVLIPLSTSMKTLSDLTKQACDNFVSRYTSEVDSISLKESELEED
                     IRSLSQQITRYENLTNNLKKHASDNQQAISSNQQIIQTLGQQKHELEEKLRKLREFNQ
                     KSPEIFKEVEEFQKIVQQGLTQAQNFWNFSTNQFNIPSGKELDWAKASHEKYLKVAMG
                     KIEHKAEKETLNKADFAVIKAYAKEHPEDDIPKSIMKYINDNKDSIKRDIGLDITSTL
                     LEQGGINASKFGVFINTAGGVKGPAGPNSFVEVKRTSGNVFIENGSKFAKGGKYLGKG
                     VAGVGFGIGMYDDLANDNKTFGEALSHNGMTLAAGSVAAFGVAALVSNPVGWAVVGIL
                     TAGIIGSTVSDYLYQNNYFGIKDKVDWAGHKIDEGIDAVNKTAEKAVDSVGNAIGDAT
                     KSISNHINPMKWSW"
     gene            complement(2557..2658)
                     /locus_tag="NBI57_002267"
     CDS             complement(2557..2658)
                     /locus_tag="NBI57_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1823196.1"
                     /translation="MCQYLCLKSVELGVVIVMIYCLKVDALTVRLHN"
     gene            2716..3156
                     /locus_tag="NBI57_002268"
     CDS             2716..3156
                     /locus_tag="NBI57_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006189740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HCD1823197.1"
                     /translation="MNLEFNIAVHVLAFLTKHHSEKFNSSSLAELTCLNPVQLRRVTT
                     QLVDLKMIDTIRGKDGGYLANDQSADVSLATLYKHFVLEKEHHTRLFTGDEGSHCQIA
                     RNIATTMSHYQQDEQNIIINFYNEKTIKDVIEDIQKEDLCHENI"
     gene            3143..>4313
                     /locus_tag="NBI57_002269"
     CDS             3143..>4313
                     /locus_tag="NBI57_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HCD1823198.1"
                     /translation="MKTYDLIVIGFGKAGKTLAKYAASTGQHVAVIEQSSKMYGGTCI
                     NIGCIPSKTLVHDGLEGKSFEASYNRKNDVVNALNNKNYHLLADDNNIDVLDFKAQFK
                     SNTEVNLLDQHGDIVDSITAPHIIINTGATSIIPNIKGLDQAKHVFDSTGLLNISYQP
                     KHLVIVGGGYIALEFASMFANLGSKVTVLERGESFMPREDQDVVAHAITDLENKGIAL
                     HTNVETTELSSDDHHTTVRTNVGNFEADAVLLAIGRKPNTALALENTDIELGDRGEIK
                     VNAQLQTSVPHIYAAGDVKGGLQFTYISLDDYRIIKSALYGNQSRTTDNRGSVPYTVF
                     IDPPLSRVGLTSKEAAAQHYDYTEHQLLVSAIPRHKINNDPRGLFKVVINNENNMI"
BASE COUNT         1453 a          765 c          606 g         1489 t
ORIGIN      
        1 gattcgcaca gcaatgttta caccccctaa ttagcaattg aaaaccaact aaatttgtaa
       61 ctatttatta gactttaaaa atatatgtac ttttttatct ataattgagt gcatattcat
      121 taacataaat agcatagtca tgaaaagcca agaaatatat aagattaaat tttgtacatt
      181 ttcaaacacc aacaagtaaa atgccccata agacatgaaa ccagtaaaga ggtaataaaa
      241 aattgtgaaa cacatatttt taaaggaggg taataatctt aatttaagtt cattattttt
      301 attttcctta taaacattta atgttaattt cttatttaga taacaataaa aaaatataac
      361 ccctaaaaaa ccaataaaac agctgctcca aacaattagt ttttctaatt gaaaatcaaa
      421 taaataagta tttcttctta cagtaccacc aagtaacaca atacccgcga atatagtcca
      481 tcctatcgat ttatttttaa ctggtttgac aatatttaat ttttcataat cattcttagt
      541 gattttgaca tatgttttcg gtatgagcca gttaataaat ggaaagaaga aaacaatcca
      601 attacttgct aaatcaatca ttaagtattc actttcgtat ttgattattc gatatttagg
      661 atttttatta ataactttag attcgcaaag caatatttac caactccatt tcataggatt
      721 aatatgatta ctaatacttt ttgtggcatc accaatggca ttaccaacac tatctacagc
      781 tttttctgca gttttgttta cagcatcaat accctcgtct attttatgtc ctgcccagtc
      841 cactttatct tttatgccaa aataattatt ttgataaaga taatcagaaa cagtagatcc
      901 aataatacct gctgttaaaa tacctaccac agcccaaccg actggattac ttactaaagc
      961 cgctacacca aatgctgcaa cagatccagc tgcaagcgtc ataccattat gcgacaatgc
     1021 ctctccaaat gttttattat catttgcaag atcatcatac atacctatac caaatcctac
     1081 acctgcaaca cctttcccta gatattttcc gccttttgca aatttactac cattttctat
     1141 aaacacatta cctgatgtac gtttgacttc cacaaatgaa tttggaccgg ctgggccttt
     1201 cactccacct gctgtattga taaatacacc gaatttactt gcatttatac cgccttgctc
     1261 taaaagtgtt gacgtaatat ctaatcctat atctcgttta atactatctt tattgtcatt
     1321 tatatatttc attatacttt tcgggatatc gtcttctgga tgttctttgg catatgcctt
     1381 tataacagca aagtctgctt tatttaaagt ttctttctct gctttatgtt caattttccc
     1441 catagcaact ttcaaatatt tttcatgact tgctttagcc caatcaagtt ctttacctga
     1501 aggtatatta aattgatttg ttgaaaagtt ccaaaaattc tgcgcttggg taagtccttg
     1561 ttggacaatt ttttgaaatt cttcaacttc tttaaatatt tctggtgatt tttgattaaa
     1621 ctcacgtaat ttacgtagtt tctcttctaa ttcatgtttt tgttgaccta atgtttgtat
     1681 tatttgttgg ttcgatgaaa tggcttgctg attatcggaa gcatgctttt tcaaattgtt
     1741 agttaaattt tcatatcgcg taatttgttg acttaatgat ctgatatctt cttcaagctc
     1801 tgattctttt aaagatatgc tatcaacctc actcgtataa cgtgacacaa aattatcgca
     1861 agcttgcttc gttaaatcac tcaatgtttt catacttgtt gataatggaa ttaacaccgt
     1921 actaaaaaat tgcttagctg acgtatacgc tttcccttta agcgcatcat cattgataaa
     1981 ttgagtaatt gctttttcca acgcatcata atttgaattc attgtttgac tcaaattccc
     2041 cacacttgaa gcttggtttc gagatctgtc taaatacatg tcaatactca tcggcatgct
     2101 cctttttcaa aaatatatga ttttcaaact atttaaaatc aaatgctttt tacatctaca
     2161 aagttgtaaa attttaaaac tcggcgatga ttatttctta tgtaaaggag tctagatgca
     2221 ggtaaattga gataacatgt cgcctttttt cttattttag catatggata taatggtgtc
     2281 tttgtatatt cgcaattaat caataaaaat tatctttcaa tattttaatt ttattgcgac
     2341 aacatcctta acattaaata tattaatatc tcaaaatata ttcactatta aaatatgtca
     2401 tcagttgtta aaagtatttc ctcatcatgc gaaatatcaa aacgtatcta aaatacgaat
     2461 aagtttatac aatcacacaa catcatcatt caaaatttta ttgctctcat tttcaatatt
     2521 catttcatat ctatcagttc atacataatt aaaatttcaa ttgtgcaatc tcaccgttaa
     2581 tgcatcaact ttcaaacaat aaatcatcac aataaccaca cctaattcaa cacttttcaa
     2641 acataagtat tgacacattg agcaaaatga tttttaattg taactaatac agttacaatt
     2701 atgaggtgag aaacattgaa tttagaattt aacattgccg tgcatgtatt agctttttta
     2761 actaagcatc attcagaaaa attcaatagt agttcattag cagaattaac ttgtttaaat
     2821 cctgttcaat tacgacgcgt gacgactcaa cttgtcgatt taaaaatgat tgacacaata
     2881 cgaggtaaag atggcggtta tttagcaaat gatcaaagtg ctgatgtctc tctagcaaca
     2941 ttatataaac attttgtctt agagaaagaa caccacacac gtctatttac tggcgacgaa
     3001 ggcagtcact gtcaaattgc tcgtaatatt gcaactacca tgtcacatta tcagcaagac
     3061 gaacagaata tcattattaa tttttataat gaaaaaacaa tcaaagatgt cattgaagac
     3121 attcaaaagg aggatttatg tcatgaaaac atatgattta attgtaatag gatttgggaa
     3181 agctggtaaa actttagcga aatatgctgc atcaacaggt caacacgtcg cagttatcga
     3241 acaatcttcg aaaatgtatg gcggcacttg tataaatata ggatgtatac cttcgaagac
     3301 acttgtacat gatggattag aaggcaaatc ttttgaagca agttataacc gtaaaaacga
     3361 tgttgtcaat gcgctaaaca ataaaaatta ccacttatta gcagacgata acaacattga
     3421 tgtattggat tttaaagcgc agtttaaatc taatactgaa gtgaatttat tagatcagca
     3481 tggcgatatc gtcgatagta ttactgcacc tcatatcatt attaataccg gtgctacctc
     3541 tatcattcct aacataaaag gccttgatca agctaaacat gtcttcgatt caacaggttt
     3601 attaaacatt agctatcaac ctaagcatct cgttattgta ggtggcggtt atattgcctt
     3661 agaatttgct tcaatgtttg cgaatttagg tagtaaggta acagtattag aacgtggtga
     3721 atcatttatg ccacgcgaag atcaagatgt cgttgcacat gctattactg acttagaaaa
     3781 taaaggcatt gcattgcata caaatgttga aacgactgaa ttgtcatctg acgatcatca
     3841 tacaacagtc cgtactaatg ttggcaactt tgaggctgat gcagtacttt tggctatcgg
     3901 gcgcaaaccg aatacagcct tagctttaga aaatactgat atcgaattag gtgatagagg
     3961 cgaaattaaa gtcaatgctc aacttcaaac atctgtgcca catatttatg ctgcaggtga
     4021 tgttaaaggc ggacttcaat ttacgtatat atctttagat gattatcgaa ttatcaaatc
     4081 agcgttatat ggtaatcagt cacgtacgac tgacaataga ggtagcgtgc cttatacagt
     4141 atttatagat ccaccattat cacgtgttgg attaactagt aaagaagctg ccgctcaaca
     4201 ttatgattac actgaacatc aacttttagt aagtgctata cctcgtcaca aaattaacaa
     4261 tgatccaaga ggtttattta aagtagtcat taataatgaa aataatatga ttt
//
LOCUS       DAHHSC010000194         4307 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.195, whole genome shotgun sequence.
ACCESSION   DAHHSC010000194 DAHHSC010000000
VERSION     DAHHSC010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 4307)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4307)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4307
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.195"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            17..4156
                     /gene="sdrD"
                     /locus_tag="NBI57_002270"
     CDS             17..4156
                     /gene="sdrD"
                     /locus_tag="NBI57_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MSCRAMM family adhesin SdrD"
                     /protein_id="HCD1823199.1"
                     /translation="MLNRENKTAITRKGMVSNRLNKFSIRKYTVGTASILVGTTLIFG
                     LGNQEAKAAESTNKELNEATTSASDNQSSDKVDMQQLNQEDNTKNDNQKEMVSSQGNE
                     TTSNGNKSIEKESVQSTTGNKVEVSTAKSDEQASPKSTNEDLNTKQTISNQEGLQPDL
                     LENKSVVNVQPTNEENKKVDAKTESTTLNVKSDAIKSNAETLVDNNSNSNNENNADII
                     LPKSTAPKSLNTRMRMAAIQPNSTDSKNVNDLITSNTTLTVVDADNSKTIVPAQDYLS
                     LKSQITVDDKVKSGDYFTIKYSDTVQVYGLNPEDIKNIGDIKDPNNGETIATAKHDTA
                     NNLITYTFTDYVDRFNSVKMGINYSIYMDADTIPVDKKDVPFSVTIGNQITTTTADIT
                     YPAYKEADNNSIGSAFTETVSHVGNVEDPGYYNQVVYVNPMDKDLKGAKLKVEAYHPK
                     YPTNIGQINQNVTNIKIYRVPEGYTLNKGYDVNTNDLVDVTDEFKNKMTYGSNQSVNL
                     DFGDITSAYVVMVNTKFQYTNSESPTLVQMATLSSTGNKSVSTGNALGFTNNQSGGAG
                     QEVYKIGNYVWEDTNKNGVQELGEKGVGNVTVTVFDNNTNTKVGEAVTKEDGSYLIPN
                     LPNGDYRVEFSNLPKGYEVTPSKQGNNEELDSNGLSSVITVNGKDNLSADLGIYKPKY
                     NLGDYVWEDTNKNGIQDQDEKGISGVTVTLKDENGNVLKTVTTDADGKYKFTDLDNGN
                     YKVEFTTPEGYTPTTVTSGSDIEKDSNGLTTTGVINGADNMTLDSGFYKTPKYNLGNY
                     VWEDTNKDGKQDSTEKGISGVTVTLKNENGEVLQTTKTDKDGKYQFTGLENGTYKVEF
                     ETPSGYTPTQVGSGTDEGIDSNGTSTTGVIKDKDNDTIDSGFYKPTYNLGDYVWEDTN
                     KNGVQDKDEKGISGVTVTLKDENDKVLKTVTTDENGKYQFTDLNNGTYKVEFETPSGY
                     TPTSVTSGNDTEKDSNGLTTTGVIKDADNMTLDSGFYKTPKYSLGDYVWYDSNKDGKQ
                     DSTEKGIKDVKVILLNEKGEVIGTTKTDENGKYRFDNLDSGKYKVIFEKPTGLTQTGT
                     NTTEDDKDADGGEVDVTITDHDDFTLDNGYYEEETSDSDSDSDSDSDSDSDSDSDSDS
                     DSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDS
                     DSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDS
                     DSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDA
                     GKHTPVKPMSTTKDHHNKAKALPETGNENSGSNNATLFGGLFAALGSLLLFGRRKKQN
                     K"
BASE COUNT         1733 a          661 c          785 g         1128 t
ORIGIN      
        1 aattaggagt aatctaatgc taaacagaga aaataaaacg gcaataacaa gaaaaggcat
       61 ggtatccaat cgattaaata aattttcgat tagaaagtac acagtgggaa cagcatcaat
      121 tttagtaggt acaacattaa tttttggtct ggggaaccaa gaagcaaagg ctgcagaaag
      181 tactaataaa gaattgaacg aagcgacaac ttcagcaagt gataatcaat cgagtgataa
      241 agttgatatg cagcaactaa atcaagaaga caatactaaa aatgataatc aaaaagaaat
      301 ggtatcatct caaggcaatg aaacgacttc aaatgggaat aaatcaatag aaaaagaaag
      361 tgtacaatct accactggaa ataaagttga agtttcaact gccaaatcag atgagcaagc
      421 ttcaccaaaa tctacgaatg aagatttaaa cactaaacaa actataagta atcaagaagg
      481 gttacaacct gatttgctag agaataaatc agtggtaaat gttcaaccaa ctaatgagga
      541 aaacaaaaag gtagatgcga aaactgaatc aactacatta aatgttaaaa gtgatgctat
      601 caagagtaat gctgaaactc ttgttgataa caatagtaat tcaaataatg aaaataatgc
      661 agatatcatt ttgccaaaaa gtacagcacc taaaagtttg aatacaagaa tgcgtatggc
      721 agcaatacaa ccaaactcaa cagattctaa aaatgttaat gatttaatca catcaaatac
      781 aacattaact gtcgttgatg cagataatag caaaacgatt gtaccagccc aagattattt
      841 atcattaaaa tcacaaatta cagttgatga caaagttaaa tcaggtgatt atttcacaat
      901 taaatactca gatacagtac aagtatatgg attgaatccg gaagatatta aaaatattgg
      961 tgatattaaa gatccaaata atggtgaaac aattgcgact gcaaaacatg atactgcaaa
     1021 taatttaatt acatatacat ttacagatta tgttgatcga tttaattcag taaaaatggg
     1081 tattaattac tcaatttata tggatgcaga tacaattcct gttgacaaga aagatgttcc
     1141 ttttagtgta actattggaa atcaaattac aactacaaca gcagatatca cttatccggc
     1201 ttataaagaa gctgacaata attcaatagg atcagctttt acagagacag tttctcatgt
     1261 aggaaatgtt gaagaccctg gttactataa ccaggtagta tatgttaatc ctatggataa
     1321 ggatttaaaa ggtgctaagt taaaagttga agcgtaccat ccgaaatatc caactaatat
     1381 tggtcaaatt aatcaaaatg ttacaaatat aaaaatatat cgtgttcctg aaggatatac
     1441 attgaataaa ggatatgacg ttaatactaa tgatttggta gacgtaactg atgaatttaa
     1501 aaataaaatg acgtatggat caaatcaaag tgttaatctt gattttggtg atattacatc
     1561 agcatatgtt gtaatggtta atacaaaatt ccaatataca aatagcgaaa gcccaacact
     1621 tgttcaaatg gctactttat cttcaacagg taataaatcc gtttctactg gcaatgcttt
     1681 aggatttact aataaccaaa gtggcggagc tggtcaagaa gtatataaaa ttggtaacta
     1741 cgtatgggaa gatactaata aaaacggtgt tcaagaatta ggagaaaaag gcgttggcaa
     1801 tgtaactgta actgtatttg ataataatac aaatacaaaa gtaggagaag cagttactaa
     1861 agaagatggg tcatacttga ttccaaactt acctaatgga gattaccgtg tagaattttc
     1921 aaacttacca aaaggttatg aagtaacccc ttcaaaacaa ggtaataacg aagaattaga
     1981 ttcaaacggc ttatcttcag ttattacagt taatggcaaa gataacttat ctgcagactt
     2041 aggtatttac aaacctaaat acaacttagg tgactatgtc tgggaagata caaataaaaa
     2101 tggtatccaa gaccaagatg aaaaaggtat atctggcgta acggtaacat taaaagatga
     2161 aaacggtaac gtgttaaaaa cagttacaac agacgcagat ggcaaatata aatttactga
     2221 tttagataat ggtaattata aagttgaatt tactacacca gaaggctata caccgactac
     2281 agtaacatct ggtagcgaca ttgaaaaaga ctctaatggt ttaacaacaa caggtgttat
     2341 taatggtgct gataacatga cattagatag tggattctac aaaacaccaa aatataattt
     2401 aggtaattat gtatgggaag atacaaataa agatggtaag caggattcaa ctgaaaaagg
     2461 tatttcaggc gtaacagtta cattgaaaaa tgaaaacggt gaagttttac aaacaactaa
     2521 aacagataaa gatggtaaat atcaatttac tggattagaa aatggaactt ataaagttga
     2581 attcgaaaca ccatcaggtt acacaccaac acaagtaggt tcaggaactg atgaaggtat
     2641 agattcaaat ggtacatcaa caacaggtgt cattaaagat aaagataacg atactattga
     2701 ctctggtttc tacaaaccga cttacaactt aggtgactat gtatgggaag atacaaataa
     2761 aaacggtgtt caagataaag atgaaaaggg tatttcaggt gtaacagtta cgttaaaaga
     2821 tgaaaacgac aaagttttaa aaacagttac aacagatgaa aatggtaaat atcaattcac
     2881 tgatttaaac aatggaactt ataaagttga attcgagaca ccatcaggtt atacaccaac
     2941 ttcagtaact tctggaaatg atactgaaaa agattctaat ggtttaacaa caacaggtgt
     3001 cattaaagat gcagataaca tgacattaga cagtggtttc tataaaacac caaaatatag
     3061 tttaggtgat tatgtttggt acgacagtaa taaagacggc aaacaagatt caactgaaaa
     3121 aggtatcaaa gatgttaaag ttattttatt aaatgaaaaa ggcgaagtaa ttggaacaac
     3181 taaaacagat gaaaatggta aataccgctt tgataattta gatagcggta aatacaaagt
     3241 tatttttgaa aagcctactg gcttaacaca aacaggtaca aatacaactg aagatgataa
     3301 agatgccgat ggtggcgaag ttgatgtaac aattacggat catgatgatt tcacacttga
     3361 taatggctac tacgaagaag aaacatcaga tagcgactca gattcggaca gcgattcaga
     3421 ctcagacagc gattcagact cagatagtga ttcagattca gatagtgatt cagattcaga
     3481 tagtgattca gattcagaca gcgactcaga ctcagatagt gactcagact cagatagcga
     3541 ttcagattca gatagcgatt cagactcaga cagcgattca gattcagaca gcgactcaga
     3601 ctcagatagc gactcagatt cggacagcga ttcagactca gatagcgact cagactcaga
     3661 cagcgattca gactcagata gcgactcaga ctcagatagc gattcagatt cagacagcga
     3721 ttcagattca gacagcgact cagattcaga tagcgattca gactcagact cagatagcga
     3781 ttcagattca gacagcgact cagattcgga cagcgactca gactcagaca gtgattcaga
     3841 ttcagatagc gactcagact cagatagcga ctcagattca gacagcgatt cagactcaga
     3901 tagtgactca gattcggaca gcgattcaga ctcagatagc gactcagatt cagacagtga
     3961 ttcagactca gatgcaggta agcacacacc tgttaaacca atgagtacta ctaaagacca
     4021 tcacaataaa gcaaaagcat taccagaaac aggtaatgaa aatagtggct caaataacgc
     4081 aacgttattt ggcggattat tcgcagcatt aggatcatta ttgttattcg gtcgtcgtaa
     4141 aaaacaaaat aaataataca gcttatacca ggtccgtgag gcctggtttt ttatttcaaa
     4201 atttatgaaa tgtatcttat gtttgagatt aaatagtgca attttttaaa aaatataaca
     4261 taaatcaaca taaatttaat tcatctttac attaaaagta caaacga
//
LOCUS       DAHHSC010000195         4295 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.196, whole genome shotgun sequence.
ACCESSION   DAHHSC010000195 DAHHSC010000000
VERSION     DAHHSC010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 4295)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4295)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4295
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.196"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            55..1056
                     /gene="argF"
                     /locus_tag="NBI57_002271"
     CDS             55..1056
                     /gene="argF"
                     /locus_tag="NBI57_002271"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HCD1823200.1"
                     /translation="MKNLRNRSFLTLLDFSRQEVEFLLTLSEDLKRAKYIGTEKPMLK
                     NKNIALLFEKDSTRTRCAFEVAAHDQGANVTYLGPTGSQMGKKETTKDTARVLGGMYD
                     GIEYRGFSQRTVETLAEYSGVPVWNGLTDEDHPTQVLADFLTAKEVLKKDYADINFTY
                     VGDGRNNVANALMQGAAIMGMNFHLVCPKELNPTDELLNRCKNIAAENGGNILITDDI
                     DQGVKVSDVIYTDVWVSMGEPDEVWKERLELLKPYQVNKEMMDKTGNPNVIFEHCLPS
                     FHNADTKIGQQIFEKYGIREMEVTDEVFESKASVVFQEAENRMHTIKAVMVATLGEF"
     gene            1079..2011
                     /gene="arcC"
                     /locus_tag="NBI57_002272"
     CDS             1079..2011
                     /gene="arcC"
                     /locus_tag="NBI57_002272"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HCD1823201.1"
                     /translation="MAKIVVALGGNALGKSPQEQLELVKNTAKSLVGLITKGHEIVIS
                     HGNGPQVGSINLGLNYAAEHNQGPAFPFAECGAMSQAYIGYQLQESLQNELHSIGMDK
                     QVVTLVTQVEVDENDPAFNNPSKPIGLFYNKEEAEQIQKEKGFIFVEDAGRGYRRVVP
                     SPQPISIIELESIKTLIKNDTLVIAAGGGGIPVIREQHDGFKGIDAVIDKDKTSALLG
                     ANIQCDQLIILTAIDYVYINFNTENQQPLKTTNVDELKRYIDENQFAKGSMLPKIEAA
                     ISFIENNPKGSVLITSLNELDAALEGKVGTVIKK"
     gene            2183..3739
                     /locus_tag="NBI57_002273"
     CDS             2183..3739
                     /locus_tag="NBI57_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000428224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HCD1823202.1"
                     /translation="MENTINESEKKKRFKLKMPGAFMILFILTVVAVIATWVIPAGAY
                     SKLSYEPSSQELKIVNPHNQVKKVPGTQQELDKMGVKIKIEQFKSGAINKPVSIPNTY
                     ERLKQHPAGPEQITSSMVEGTIEAVDIMVFILVLGGLIGVVQASGSFESGLLALTKKT
                     KGHEFMLIVFVSILMIIGGTLCGIEEEAVAFYPILVPIFIALGYDSIVSVGAIFLASS
                     VGSTFSTINPFSVVIASNAAGTTFTDGLYWRIGACIVGAIFVISYLYWYCKKIKNDPK
                     ASYSYEDKDAFEQQWSVLKDDDSAHFTLRKKIILTLFVLPFPIMVWGVMTQGWWFPVM
                     ASAFLIFTIIIMFIAGTGKSGLGEKGTVDAFVNGASSLVGVSLIIGLARGINLVLNEG
                     MISDTILHFSSSLVQHMSGPLFIIVLLFIFFCLGFIVPSSSGLAVLSMPIFAPLADTV
                     GIPRFVIVTTYQFGQYAMLFLAPTGLVMATLQMLNMRYSHWFRFVWPVVAFVLIFGGG
                     VLITQVLIYS"
     gene            4046..4273
                     /locus_tag="NBI57_002274"
     CDS             4046..4273
                     /locus_tag="NBI57_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1823203.1"
                     /translation="MTHLTKVLDTLTGICVVLLFSKYFVAYANMVFDWNLRWYLLENI
                     PHLPIILFILMFIFGVPSEMIKDRQRKNNGV"
BASE COUNT         1466 a          587 c          842 g         1400 t
ORIGIN      
        1 attttgtatg cgcttacaat ttaggtgtaa ctaaaataaa aggagttgtt attaatgaaa
       61 aatttacgaa acagaagttt tttaacttta ttagactttt cacgacaaga ggtagaattc
      121 ttattaacac tctccgagga tttaaaacgt gctaaatata ttggcactga aaagcctatg
      181 ttaaaaaata aaaatattgc actgttattt gaaaaagatt ctacaagaac gcgatgtgca
      241 tttgaagttg cagcgcatga tcaaggtgca aatgtaactt atttaggccc aactggatca
      301 caaatgggta aaaaagaaac aactaaagat actgcacgtg tgcttggtgg aatgtatgat
      361 ggcattgaat accgtggttt ttcacaaaga acagtagaaa ctttagctga atattcaggc
      421 gtaccagtgt ggaatggttt aactgatgaa gatcatccta ctcaagttct tgctgatttc
      481 ttaacagcaa aagaagtctt aaaaaaagat tatgcagata ttaactttac atatgttgga
      541 gatggtcgta ataacgttgc aaatgcatta atgcaaggtg ctgccattat gggtatgaac
      601 ttccatttag tttgtccaaa agaattaaat ccaacagatg aattattaaa tcgctgtaaa
      661 aatattgccg ctgaaaatgg tggcaacata ttaatcacag atgatattga ccaaggtgta
      721 aaagtttcgg atgtaattta cactgatgtt tgggtatcaa tgggtgaacc tgatgaagta
      781 tggaaagaac gacttgaatt attgaaacca tatcaagtaa ataaagaaat gatggataaa
      841 actggtaatc caaatgttat ttttgagcat tgcttaccat ctttccataa tgctgatacg
      901 aaaattggtc aacaaatttt tgaaaaatat ggtattcgag aaatggaagt tacagatgaa
      961 gtattcgaaa gtaaagcttc agttgtattc caagaagctg agaacagaat gcatacaatc
     1021 aaagcagtca tggttgctac attgggtgaa ttttaaatga tataaggaag tgaatatgat
     1081 ggcgaaaata gtagtagcat taggtggtaa tgctttagga aaatcacctc aagaacaact
     1141 cgagcttgtt aaaaatactg cgaaatcatt agtaggatta ataacaaaag gacatgagat
     1201 tgttattagt catggtaatg gaccacaggt tggaagcatt aatttgggac ttaactatgc
     1261 tgcagaacat aaccaaggtc cggcatttcc atttgctgaa tgtggcgcaa tgagtcaagc
     1321 ttacatcggc tatcaattac aagaaagctt acaaaatgaa ttgcattcta ttggaatgga
     1381 taaacaagtg gtaacactag tgacacaagt tgaagttgat gaaaatgatc cggcatttaa
     1441 caatccttca aaaccaattg ggttatttta caacaaagaa gaagctgaac aaattcaaaa
     1501 agaaaaagga tttatatttg ttgaagatgc tggaagagga tatagacgcg ttgttccttc
     1561 accacaaccc atctctatta ttgaattaga gagtattaaa acacttatta aaaatgatac
     1621 actcgttatt gctgctggtg gtggaggtat accagtaatt agagagcaac atgatggttt
     1681 taaaggtatt gatgcagtta tagacaaaga taaaacaagt gcgttgttgg gtgctaatat
     1741 tcaatgcgat caattgatta ttttaacagc aattgattat gtatatatta attttaacac
     1801 tgaaaaccaa cagcctttga aaacaacaaa tgttgatgaa ttaaaacgat atatagacga
     1861 aaatcaattt gcaaaaggaa gtatgttacc aaaaattgaa gcagccatat catttattga
     1921 aaacaatcca aaaggaagtg tgcttataac atcattaaat gaattagatg ctgccttaga
     1981 gggtaaagta ggtactgtga ttaaaaagta attgaattga aacgcttttc aattactata
     2041 tgtcaaatgc atgattttta ttatttatgt gcaccccctg aaaataatgc ctctattttg
     2101 atgcggggtg cactttctta atttatattt ataaaatctt taaggtagaa atgctaggtt
     2161 aaatgtcgaa ggagatgaaa ccgtggaaaa tacaattaat gaaagtgaaa agaaaaaacg
     2221 atttaaatta aaaatgccag gtgcatttat gattttattc attttaacgg ttgttgcagt
     2281 tatagcaaca tgggttattc ctgctggtgc atattctaaa ctttcttacg aaccttcatc
     2341 ccaagaacta aagatagtta accctcataa ccaagtgaaa aaggttccgg gtacgcaaca
     2401 ggaactagac aaaatggggg ttaaaattaa gattgaacaa tttaaatcag gtgcaattaa
     2461 taagccagta tcaattccga atacttatga aagattaaag caacatccag ctggaccaga
     2521 acaaataaca agtagcatgg ttgaaggtac gatagaagcg gtcgatatca tggtattcat
     2581 tcttgtacta gggggactta ttggcgtagt tcaagccagt ggttcttttg aatcgggatt
     2641 gttagcttta acgaagaaaa caaaagggca tgaatttatg ctaattgtgt ttgtatcaat
     2701 actaatgatt atcggcggga cgttatgtgg tattgaagaa gaagctgtag cattctatcc
     2761 gattttagtc cctatattta tagcgttagg atacgattct atcgtttcag ttggtgccat
     2821 attccttgcc agctctgtcg gtagtacatt ttcaactatt aacccgttct cggttgtaat
     2881 tgcctctaat gccgctggta caacttttac ggatggcttg tattggagaa taggtgcttg
     2941 tattgtcggt gcgatttttg ttattagtta tttatattgg tactgtaaaa aaattaaaaa
     3001 cgatcctaaa gcgtcatatt cttatgaaga caaagatgct tttgaacagc aatggtctgt
     3061 attaaaagat gacgatagtg cccattttac tttgcgtaag aagataatcc ttacattatt
     3121 tgtactacca tttccaatta tggtatgggg agttatgacg caaggttggt ggttcccagt
     3181 tatggcttca gcatttttaa tatttacaat tataataatg tttattgctg ggacaggtaa
     3241 atctggattg ggagaaaaag gaactgtaga tgcatttgtc aatggtgcat caagtttagt
     3301 aggtgtatct ttaattattg gtttagctcg aggtattaat ttagtgttga atgaaggtat
     3361 gatttcagat acaatcttac acttttcatc atctttagtt caacatatga gtggaccatt
     3421 atttatcatc gtattactat ttattttctt ctgtttaggt tttatcgtgc catcatcttc
     3481 tggattagcg gtattatcaa tgcctatctt tgcaccacta gctgatacag taggtatacc
     3541 aagattcgtc atcgttacga catatcaatt tggtcaatat gcgatgttat tcttagcgcc
     3601 gactggactt gttatggcca cactacaaat gttaaacatg cgatattcac attggttccg
     3661 atttgtatgg ccggtagttg cttttgtatt gattttcggt ggcggagtac taattacgca
     3721 agtactaatt tattcataat ttgaaatgct atattataaa aatactaatt gggttttatg
     3781 catctcgtag gtttgtagaa atactaaact aagcgaggtg cattattatt tttgattaag
     3841 aaaataatga cggtaatgat aacactagta agtaattgat acaatgctct atttaataat
     3901 gatattttta aaatttgttt ttaatgtaat gttagatcta tggtatatta tatttatcgt
     3961 ggtaaatatg tatttgctgt aaacagtttt tgtaacacat gacacatatg gttgtgaaaa
     4021 tagcatatat aaaggatggc tataaatgac acatttgaca aaggttttag atacactaac
     4081 tggaatatgc gtagtattat tatttagtaa atattttgtg gcgtatgcaa atatggtgtt
     4141 tgattggaat ttaagatggt atttgctaga aaacatacca catttgccaa ttatattatt
     4201 tattctgatg tttattttcg gagtaccttc tgaaatgata aaagataggc aaaggaaaaa
     4261 taacggtgtt taatttatcg atatttagag gtgat
//
LOCUS       DAHHSC010000196         4285 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.197, whole genome shotgun sequence.
ACCESSION   DAHHSC010000196 DAHHSC010000000
VERSION     DAHHSC010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 4285)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4285)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4285
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.197"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            368..1177
                     /locus_tag="NBI57_002275"
     CDS             368..1177
                     /locus_tag="NBI57_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCD1823204.1"
                     /translation="MCGLRSITLGTTNIEQTKHFMVDILGLNYEELLENSIRFGDADI
                     SPGTRLQFIQVPSEQLEESHFVGIGLRTPTDSGLEEYAEILSNKDIPFTTVKELNGNK
                     YFSLEDNNGHIFSIYSNENNYGVGLGMPSFESAVNPLHQVQGLGPVILKVNHVDITGQ
                     ILTNIFGLEVFAEYQPFDNADYHVQVFKVGTGGLGGEIHLMPVETEMTMPEYGAVDQV
                     EFETKDADFFNQAKSRLDEVEIPYQTLEQDDIESIRITENSGLSFIFTLQK"
     gene            1206..1409
                     /locus_tag="NBI57_002276"
     CDS             1206..1409
                     /locus_tag="NBI57_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499941.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1823205.1"
                     /translation="MLHETWKERTPIKKVEVINTDAKKFTVSDMLTVGKQYDVINETE
                     EYYQIIDNSGLVGGYYKTYFKEV"
     gene            1590..2381
                     /locus_tag="NBI57_002277"
     CDS             1590..2381
                     /locus_tag="NBI57_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HCD1823206.1"
                     /translation="MALKHYKNSDSTVFNDAKALFDLNKNILLKGPTGSGKTKLAETL
                     SEVVDTPMHQVNCSVDLDTESLLGFKTIKTNAEGQQEIVFVDGPVIKAMKEGHILYID
                     EINMAKPETLPVLNGVLDYRRQITNPYTGEVIKAVPGFNVIAAINEGYVGTLPMNEAL
                     KNRFVVIHVDYIDGDILKNVIKEQSLLQDDKQIEQIIKFNEDLRTMSKQGQISEEAAS
                     IRALLDLCDLITVMPVERAIKRTIIDKLEDEREQQAIYNAVELNF"
     gene            2395..4281
                     /locus_tag="NBI57_002278"
     CDS             2395..4281
                     /locus_tag="NBI57_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide reductase activation protein NorD"
                     /protein_id="HCD1823207.1"
                     /translation="MSDRFIKFNDEQLDAKQVMMLQDLARLLLKNEQTQVKIQKFPYY
                     NPVQNVLITSWFWSHRPSHIEMAGLKTDVMLAAYGYHMMDVQIVNEVVQDKTFKHPKF
                     YQQLFKLLEDMRVLNSIKVERPSTAKLIDLRLDTRVSYTESQIKVYRTKTQYTDLLFL
                     YLEHAFLSQDFFDIPSIHSDLDDILVNMFLYLPNFFQNQNSEDNMYLAQRIMYQVDDI
                     LKEDMLNEYYYLPKTLYNTLASPEFDDLKRTDASQVDGQDDTSEDDDNESEKADSKSA
                     DSESKGGAYLEMELHEGQNSETLGNDEAREGDATDDMTDMMTKKGKGSNDTLNREEGD
                     AVGQSQAFQLDGVNKNVEIKWQIPEIEPQYVLEYQESKQDVQYEIKDLIQIIKKTIER
                     EQRDARFNLTKGRLQKDLINWFIDDQYKLFYKKQDLSKSFDATFTLLIDASASMHDKM
                     AETKKGVVLFHETLKALNIKHEILSFSEDAFDSDDHAQPNIINEIINYDYSTFEKDGP
                     RIMALEPQDDNRDGVAIRVASERLMRRNQHQRFLIVFSDGEPSAFNYSQDGIIDTYEA
                     VEMSRKFGIEVFNVFLSQDPITEDVEQTIHNIYGQYAIFVEGVAHLPGHLSPLLKKLL
                     LKSL"
BASE COUNT         1624 a          584 c          749 g         1328 t
ORIGIN      
        1 tcaatatgct aatgaaatca ttttagataa taacaagcga tgaaatttat gaattctata
       61 agcgccttta gttaatttaa aatcatctga acaagatgta aattcacttt atggatacga
      121 catgtatatg ttttttatgt actacataaa acgaaatatt caattgctat atacagatac
      181 tcattaatga catactcaat agcaaccata acaatattat gttacctatt taaatgaaat
      241 agttctttta aagaaataca tttttcacat attaatctat aatcaaaatc aactgaccga
      301 tattctataa tttatgatta aaataagtta taatataata gtaaagataa agatagaggt
      361 ggctataatg tgtggactta gaagtataac attaggtaca acaaatatag aacagacaaa
      421 acatttcatg gttgacatat taggattaaa ttatgaagaa cttcttgaaa actcaattcg
      481 tttcggcgat gcagatataa gcccaggaac aagacttcaa tttatacaag ttccaagtga
      541 gcaattagaa gaatctcact ttgtgggtat tggattacgt acaccaactg actcaggttt
      601 agaggagtat gcggaaatat tatcgaataa ggatattcca tttacaacag ttaaagaatt
      661 aaatggcaat aaatatttca gtctcgaaga taacaatggt catattttct caatatattc
      721 aaacgagaat aattatggcg ttggtttagg tatgccttct tttgagagtg cggtcaatcc
      781 gttacatcaa gtgcaaggtt taggaccagt gattcttaaa gtgaatcatg tagatattac
      841 aggtcaaatt ttaacaaata tattcggact tgaagtattt gcagaatacc aacccttcga
      901 caatgctgac tatcatgtcc aagtattcaa agttggaacg ggtggtctag gtggcgaaat
      961 acatttaatg cctgtagaaa cagaaatgac aatgccggaa tatggcgcag tcgatcaagt
     1021 tgagtttgaa acgaaagatg cagatttctt taatcaagcg aaatcgcgct tagatgaagt
     1081 ggaaatacca tatcaaacgc ttgagcaaga tgatattgaa tcaattagaa ttactgaaaa
     1141 cagtggatta tcgtttatat tcactttaca aaaataattt ttttacgata gtgaggacaa
     1201 aatttatgtt acatgaaact tggaaagaac gtacaccaat caagaaagta gaagtcatta
     1261 atacagatgc aaagaaattt acagtttctg acatgttaac ggttggcaaa caatatgatg
     1321 ttatcaacga aactgaagaa tattatcaaa taatagataa ttctggatta gttggcggtt
     1381 actataaaac atatttcaaa gaagtttaaa cgcataatta ttgaaatgaa gcctaatatg
     1441 agattttcaa ttagtaaatt tggtaaaata taaacaattc gcatactaag tattgcgttt
     1501 tatatcagaa tagaatagta aactaaggca tttgaagaac ttattccatg tcttagtttt
     1561 ttaatttatt taaaggagaa cgttttaaaa tggcactaaa acattataag aattcagatt
     1621 caacagtttt caatgatgcg aaggcattat ttgatttaaa taaaaatatt ttacttaaag
     1681 gtccaacagg ttcagggaaa acaaagttgg cagaaacatt aagtgaagtt gttgatacac
     1741 ccatgcatca agtcaattgt tctgttgatt tagatacaga aagcttatta ggctttaaaa
     1801 caattaaaac aaatgcggaa ggtcaacaag aaattgtctt tgtagatggt ccagttatta
     1861 aagctatgaa agaggggcat attttatata ttgatgaaat aaatatggct aaacctgaaa
     1921 cattgcctgt attaaatggt gtattagatt atcgtcgtca aattacgaat ccatacactg
     1981 gtgaagtaat caaagctgta ccaggattta acgttatagc agcgataaat gaaggttatg
     2041 ttggtacttt gccaatgaat gaagcactaa aaaatcgctt tgttgttatt cacgttgatt
     2101 atattgacgg ggacatttta aaaaatgtga ttaaagagca aagtttatta caagatgata
     2161 aacaaatcga acaaattatt aagtttaacg aagatttacg tactatgtct aagcagggac
     2221 aaatttctga agaagccgct agtatccgtg cattattaga cttgtgtgat ttaatcactg
     2281 taatgccagt tgaacgtgca attaaacgta caattattga taaattggaa gatgaacgtg
     2341 aacaacaagc aatatataat gctgtagaac taaactttta aagagggata aaacatgagt
     2401 gatcgtttca taaaatttaa cgacgaacag ttagatgcca agcaagtaat gatgttgcaa
     2461 gacttagctc gactcttatt aaaaaatgaa caaacacaag ttaaaatcca aaaattccct
     2521 tactataatc ctgttcaaaa tgttttgatt acgagttggt tttggtcaca tcgaccaagc
     2581 catatcgaaa tggctggttt aaaaacagat gttatgttag ctgcttacgg ttatcatatg
     2641 atggatgtac aaattgtaaa tgaagtagta caagataaaa cttttaaaca ccctaaattt
     2701 tatcaacaac tttttaaatt attagaagat atgcgcgtat taaattctat taaagttgag
     2761 cgcccaagta ctgctaaatt aattgatctt cgcttagata cacgggtatc atatacggaa
     2821 tctcaaatta aagtgtatcg tactaaaaca caatatacag atttattatt tttgtattta
     2881 gaacatgcat ttttaagtca agatttcttt gatataccat ctatacattc agatttagat
     2941 gatattttag taaatatgtt cttatattta cctaattttt tccaaaatca aaattctgaa
     3001 gataatatgt atctggctca aagaattatg tatcaagtag atgatatttt aaaagaagat
     3061 atgctaaacg aatattatta cttacctaaa acactatata atacattagc tagtccagag
     3121 tttgatgatt taaagagaac tgatgcaagt caagtcgatg ggcaagatga tacttcagaa
     3181 gatgatgata acgaaagtga aaaagctgac tctaaatctg cagatagtga gtcaaaaggt
     3241 ggcgcctatt tagaaatgga gcttcacgaa ggacaaaata gtgaaacatt aggtaatgat
     3301 gaagcacgtg aaggcgatgc tacagatgat atgactgata tgatgaccaa aaagggcaaa
     3361 ggatcaaacg atacgctaaa tcgtgaagaa ggcgatgctg ttggtcaaag ccaagcattt
     3421 caacttgatg gtgtgaacaa aaatgtagaa ataaaatggc aaattcctga aattgagcca
     3481 caatatgttt tagaatatca agaatcgaaa caagacgtac aatatgaaat caaagactta
     3541 attcaaatta taaagaaaac gattgaacga gaacaacgag atgcacgttt taatttaaca
     3601 aaagggcgat tacaaaagga tttaattaat tggtttatcg acgatcaata taaattgttt
     3661 tacaaaaaac aagatttaag taaatcattt gatgcgacgt ttacattgtt aattgatgcg
     3721 tcagcaagta tgcatgataa aatggctgaa acgaagaaag gtgtagtgtt attccacgag
     3781 acgttaaaag cattgaacat taaacatgaa attttatcat tcagtgagga tgcctttgat
     3841 tcagacgatc atgcacaacc aaatatcatt aatgaaatta ttaactatga ttactcaacc
     3901 tttgaaaaag atggcccacg tattatggca cttgaacctc aagatgataa tcgtgatggt
     3961 gttgctatta gagttgcaag tgaacgatta atgcgtcgaa atcaacatca acgattttta
     4021 atcgtatttt cagacggcga accgtctgca tttaattata gtcaagatgg tattattgat
     4081 acgtatgaag ctgtagaaat gtcacgtaaa tttggtattg aagtatttaa tgtatttttg
     4141 agtcaagatc caataactga agatgttgaa caaacaattc ataatattta tggtcaatat
     4201 gctattttcg ttgaaggtgt tgcgcattta cctggacatc tctctccatt attaaaaaaa
     4261 ttactactta aatctttata aataa
//
LOCUS       DAHHSC010000197         4278 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.198, whole genome shotgun sequence.
ACCESSION   DAHHSC010000197 DAHHSC010000000
VERSION     DAHHSC010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 4278)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4278)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4278
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.198"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            complement(104..382)
                     /gene="qoxD"
                     /locus_tag="NBI57_002279"
     CDS             complement(104..382)
                     /gene="qoxD"
                     /locus_tag="NBI57_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome aa3 quinol oxidase subunit IV"
                     /protein_id="HCD1823208.1"
                     /translation="MKHTVGFIASIVLTLLAVYVTLYTSLTFHAKLTIIFGFAFVQAG
                     LQLLMFMHLTEGKDGRLQTFKVIFALVITLCFVVGTYWVMQGGHSSHL"
     gene            complement(391..996)
                     /gene="qoxC"
                     /locus_tag="NBI57_002280"
     CDS             complement(391..996)
                     /gene="qoxC"
                     /locus_tag="NBI57_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome aa3 quinol oxidase subunit III"
                     /protein_id="HCD1823209.1"
                     /translation="MSHDTNTIDSRTHEGELNKLGFWIFITAEFALFGTLFATLLTLQ
                     HGGDYAGKMTTELFELPLVLIMTFALLFSSYTCGIAIYYMRQEKQKLMMFWMIITLLL
                     GLVFVGFEIYEFAHYASEGVNPTIGSYWSSFFILLGTHGCHVSLGIVWAICLLIQIQR
                     RGLDKYNAPKLFIVSLYWHFLDVVWVFIFTAVYMIGMVYSG"
     gene            complement(986..2974)
                     /gene="qoxB"
                     /locus_tag="NBI57_002281"
     CDS             complement(986..2974)
                     /gene="qoxB"
                     /locus_tag="NBI57_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001010769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome aa3 quinol oxidase subunit I"
                     /protein_id="HCD1823210.1"
                     /translation="MNFPWDQLLVKGNWMITMAQIGAPFLVIGLIAVITYFKLWKYLY
                     KEWFTSVDHKKIGIMYLICAVLMFVRGGIDALLIRAQLTVPDNKFLESNHYNEIFSTH
                     GVIMIIFMAMPFIFGLWNIVVPLQIGARDVAFPVLNNVSFWLFFAGMILFNLSFIIGG
                     SPAAGWTNYAPLAGEFSPGPGVNYYLIAIQISGLGTLATGINFFVTILRCKTPTMKFM
                     QMPMFTVTTFITTLIVILAFPPLTVALALMTTDRIFDTAFFTVAHGGMPMLWANFFWV
                     WGHPEVYIVILPAFGIYSEIIPTFARKRLFGHQSMVWATAGIAFLSFLVWVHHFFTMG
                     NGALINSFFSISTMLIGIPTGVKLFNWLLTLYKGRITFESPMLFSLAFIPNFLLGGVT
                     GVMLAMASADYQYHNTYFLVAHFHYTLVTGVVFACLAGLIFWYPKMMGYKLNETLNKW
                     CFWFFMIGFNVCFLPQFILGLDGMPRRLYTYMPSDGWFLLNLISTIGALLMAIGFLFL
                     VVSIVYSHFKSPREATGDNWDGLGRTLEWTTASAIPPKYNFAITPDWNDYDTFVDMKE
                     HGRHYLDNHNYKDIHMPNNTPVGFWIGIFMTIGGFFLIFETVIPALICLFGIFGTMIY
                     RSFQIDHGYHIPAAEVAETEARLREARIKEREAVSHES"
     gene            complement(2974..4074)
                     /gene="qoxA"
                     /locus_tag="NBI57_002282"
     CDS             complement(2974..4074)
                     /gene="qoxA"
                     /locus_tag="NBI57_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome aa3 quinol oxidase subunit II"
                     /protein_id="HCD1823211.1"
                     /translation="MSKFKSLLLLFGTLILLSGCSNIEIFNAKGPVASSQKFLILYSI
                     VFMLVICFVVLGMFAIFIYKYSYNKNAESGKMHHNAIIETIWFVIPIIIVAALAIPTV
                     KTLYDYEKPPKSEKDPMVVYAVSAGYKWFFAYPDEHIETVNTLTIPKDRPVVFKLQAM
                     DTMTSFWIPQLGGQKYAMTGMTMNWTLEASQTGTFRGRNSNFNGEGFSRQTFKVNAVS
                     QKDYDKWVKEVKGKKTLDQDTFDKQLLPSTPNKALEFNGTHMAFVDPAADPEYIFYAY
                     KRFNFELKDPNFTSEENMFKDVSDKPLIPARKAQITNANYKRHGMKLMILGNDEPYNN
                     EFKKDESKNAKEMKKISKDAQDQDNDDHGGGH"
BASE COUNT         1460 a          766 c          745 g         1307 t
ORIGIN      
        1 tataagatat agaaggggac ttaaccattt aaaaattgaa ggaaaataaa aaccagctaa
       61 caaaagtgtt agctggttaa actttacatt attaaaatgt tgtttataag tgtgaagagt
      121 gaccgccttg cataacccaa tatgttccga caacgaaaca aagtgtaatt acaagagcaa
      181 agataacttt gaatgtttgt aaacgtccat ctttaccttc agttaaatgc atgaacatta
      241 ataattgaag tcctgcttgg acgaatgcaa agccaaagat aattgtcaac ttcgcgtgga
      301 atgttaatga cgtgtatagt gttacgtaaa ctgctaaaag cgttaatacg atagatgcga
      361 taaatcctac agtatgtttc attattgtac tcatccgcta tacaccatcc ctatcatata
      421 tacggcagta aagatgaaaa cccaaacaac atctaagaag tgccagtata aacttactat
      481 aaataatttt ggcgcattat atttgtctaa tccgcgtcgt tggatttgga ttaataaaca
      541 aatggcccaa acgataccta gcgatacgtg acaaccatgc gttcctaata ggataaagaa
      601 actagaccag taagaaccaa ttgttgggtt aacgccttct gatgcatagt gtgcgaattc
      661 ataaatttcg aatccaacaa agactaaacc taaaagtaac gtaatgatca tccaaaacat
      721 cattaacttt tgtttttctt ggcgcatgta gtaaatagca ataccacatg tgtaagaact
      781 gaataataat gcaaacgtca ttattaaaac aagaggcaat tcaaataact cagtagtcat
      841 tttacctgca taatcgccac catgttgcaa agttaatagt gtcgcaaata gggtaccgaa
      901 taacgcaaat tcggctgtaa tgaaaatcca aaagccaagc ttatttaatt cgccttcatg
      961 tgtgcgtgaa tcaatagtgt ttgtatcatg actcatgact tacagcctcc ctttctttaa
     1021 tacgcgcttc tcttaatcta gcttcagttt cagcaacttc agcagcaggg atatgatatc
     1081 cgtgatcgat ttggaaactg cgataaatca tagtaccaaa aataccgaat aaacaaatta
     1141 atgctggaat tacagtttcg aaaattaaga agaaaccgcc gatagtcata aagataccaa
     1201 tccagaatcc aacaggagta ttgtttggca tatgaatgtc tttgtaatta tggttgtcta
     1261 agtaatgacg accatgttct ttcatatcaa caaatgtgtc gtagtcattc caatctggtg
     1321 taatggcaaa gttgtattta ggtggaattg ctgatgcagt agtccactct agagtacggc
     1381 caaggccatc ccagttatct ccagttgctt cacgtggaga tttgaagtga ctgtatacga
     1441 tactaacaac aaggaataag aatccgattg ccattaataa tgcaccgata gttgaaatta
     1501 agtttaataa gaaccaacca tctgatggca tataagtgta taaacgacgt ggcataccat
     1561 ctaatccaag aatgaattgt ggtaagaaac aaacgttaaa tccgatcatg aagaaccaga
     1621 agcaccattt gtttaatgtt tcgtttaatt tgtaacccat catctttgga taccagaaga
     1681 ttaaaccagc taagcaggca aatacaacac cagtaaccaa tgtatagtgg aagtgagcta
     1741 ctaagaagta cgtattgtga tattgatagt cagctgatgc cattgctaac attacacccg
     1801 taacaccacc taaaaggaag ttagggataa atgctaatga gaatagcatt ggtgactcaa
     1861 atgtaatacg tcctttatat aatgttaata accagttaaa caatttcaca ccagttggaa
     1921 taccaatcag cattgttgaa attgagaaga atgagttgat taacgcacca ttacccattg
     1981 tgaagaaatg gtgaacccaa actaagaaac taaggaacgc gataccggca gttgcccata
     2041 ccatactttg atgtccgaat aaacgcttac gagcgaatgt cgggataatt tctgagtaaa
     2101 taccaaatgc tggaaggata acgatataaa cttcagggtg cccccatacc cagaagaagt
     2161 tagcccaaag cattggcata ccgccatgtg caactgtgaa gaatgctgtg tcaaatattc
     2221 tatcagttgt cattaatgct aacgctactg ttaaaggagg gaaagcaaga ataacaatta
     2281 atgtagtaat aaatgttgtt actgtaaaca ttggcatttg cataaacttc atagttggtg
     2341 ttttacatct taaaattgtt acaaagaagt tgatacctgt agctaaggta ccaagccctg
     2401 aaatttgtat agctattaag taatagttaa cacccggacc aggactgaat tcacctgcta
     2461 gtggcgcata gtttgtccaa ccagctgctg gtgaaccacc aataataaat gacaggttga
     2521 ataaaatcat acctgcaaag aatagccaga aacttacgtt gtttaatact gggaatgcaa
     2581 catcacgtgc tccaatttgt aatggaacaa cgatattcca taaaccaaag ataaatggca
     2641 ttgccatgaa gataatcatg attacaccat gtgtactaaa aatttcgtta tagtggttag
     2701 attctaaaaa tttgttatca ggtactgtta attgcgcacg aataagtaac gcatcaatac
     2761 caccacggac gaacattaat acggcacaga ttaaatacat aataccgatt ttcttatggt
     2821 ctacagatgt gaaccattct ttgtaaagat atttccataa tttaaagtaa gtaattactg
     2881 cgattaaacc aataactaag aatggggcac caatttgtgc cattgtaatc atccagttac
     2941 ctttaactag taattgatcc catggaaaat tcattaatgt ccacctccat gatcatcatt
     3001 gtcttgatct tgcgcatctt ttgaaatttt cttcatttct ttcgcatttt tcgattcatc
     3061 tttcttgaac tcattgttat atggttcgtc atttccaaga atcatcaact tcataccatg
     3121 tcgtttatag ttcgcatttg taatttgagc tttacgagca ggtattaatg gtttgtctga
     3181 tacatcttta aacatatttt cttcactagt gaagtttgga tctttcaatt cgaaattgaa
     3241 acgtttatat gcatagaaga tgtattctgg atcggctgct ggatcaacaa acgccatatg
     3301 tgtaccatta aattctaaag ctttattagg tgtgcttggt aataattgtt tatcaaatgt
     3361 atcttgatct aacgttttct tacctttaac ttctttcacc catttgtcgt agtctttttg
     3421 actaacggca tttactttaa atgtttgacg tgagaatcct tcaccattga agttagagtt
     3481 acgacctctg aacgtaccag tttgagatgc ttctaacgtc caattcattg tcatgccagt
     3541 catggcatat ttttgaccac ctaattgtgg aatccagaaa cttgtcattg tatccatagc
     3601 ttgaagctta aatacgacag gacgatcttt agggattgtt aatgtattaa cagtctctat
     3661 atgttcatct ggataagcaa agaaccattt gtatcctgca cttactgcat atacaaccat
     3721 tggatctttc tcactcttcg gtggtttttc gtaatcgtat aaagttttaa ctgtaggaat
     3781 agctaaagca gcaacgatta tgataggtat tacaaaccat attgtttcaa tgatggcatt
     3841 atggtgcatc ttaccagatt cggcattctt attataacta tacttgtaaa taaaaatggc
     3901 gaacatgcca agtacaacga aacaaataac aagcatgaag acgattgaat aaagaatcaa
     3961 gaacttctga ctacttgcta ctggcccttt tgcgttgaaa atttctatat ttgaacaacc
     4021 actaagtaaa attagtgtgc caaataatag aagcaaagac ttaaattttg acactttttt
     4081 gacctcctaa tactacaaat gtagggctta acattaattt taagttatta cacaatattt
     4141 acaagggctt atgggaaaaa aattaataaa attgtatcaa aaatgttgat aaatcaaggt
     4201 gtgacgtggg ttcacacatt tgttaaaatt atgtgtacat tttgtgacta atagcgtttt
     4261 tgaaaaaatt gcttcata
//
LOCUS       DAHHSC010000198         4242 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.199, whole genome shotgun sequence.
ACCESSION   DAHHSC010000198 DAHHSC010000000
VERSION     DAHHSC010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 4242)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4242)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4242
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.199"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            15..608
                     /locus_tag="NBI57_002283"
     CDS             15..608
                     /locus_tag="NBI57_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HCD1823212.1"
                     /translation="MATNHEIEFKQMITASIYNKLQEKYFKDSVLFKQVNYYIDTPDF
                     KLKEHRSALRIRVKDNQYEMTLKTPAKVGLLEYNYIVDIKPEMNLIISNDNLPDDIRQ
                     IIVEQFGVKDTTLSILGALTTYRQETKYKGDLLVLDKSEYFDTADYELEFEVKDYNQG
                     LQKFQSLLNELNLEHHQPLNKVQRFFKKKETLSNNIN"
     gene            712..1077
                     /locus_tag="NBI57_002284"
     CDS             712..1077
                     /locus_tag="NBI57_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000214067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="truncated hemoglobin"
                     /protein_id="HCD1823213.1"
                     /translation="MTTTPYDIIGKEALYDMIDYFYTLVEKDERLNHLFPGDFAETSR
                     KQKQFLTQFLGGPNIYTEEHGHPMLRKRHMDFTITEFERDAWLENMQTAINRAAFPQG
                     VGDYLFERLRLTANHMVNS"
     gene            1100..1906
                     /gene="yjbH"
                     /locus_tag="NBI57_002285"
     CDS             1100..1906
                     /gene="yjbH"
                     /locus_tag="NBI57_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease adaptor protein YjbH"
                     /protein_id="HCD1823214.1"
                     /translation="MAGELRIMENKSREDINLSPVSKIEIYSFFDPFSSDCFKLSAIL
                     SKLRIEYNQYIRIRHILNPSLKVLTKCQAQSTSNFDNIALAYKAAELQGRVRAERFIH
                     LMQNEIIPKRDIITESMICDCIQNAGIDLEVFKDDLQKSKLTESLKIDLHIAREMEIE
                     QAPSLVFFSEDVHEEGLKVEGLYPYHIYTYIINELMGKPIEKNLPPKLETYIQQQQLV
                     TMEELLTIYEWPEKLLNKELKKLAIQQKIEKLKYPDGDFWKSKMPKIKSK"
     gene            complement(2366..4174)
                     /gene="pepF"
                     /locus_tag="NBI57_002286"
     CDS             complement(2366..4174)
                     /gene="pepF"
                     /locus_tag="NBI57_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HCD1823215.1"
                     /translation="MSQQLSREEQERKYPEYTWDLTTIFKDDEAFEAAFKEVENELGK
                     EEQFKGHIGDSAETLYNALELEDTLGTKLEKVYVYAHLKQDQDTTNDKYTGMESRAHQ
                     LIIKFSSAWSFLVPEILQIDEDKIQSFVNSYDKLQKFAFDLKLINEKRPHILDAETEK
                     LLTEAQDALSTPSNVYGMFSNADLVFEDAIDKDGNAHPLTQGTFIKYLESDDRKLRES
                     AFRNVYKAYGAHNNTLGATLAGEVKKNVFNARTHNYKTAREKALSNNHIPENVYDNLV
                     KTVHKYLPLLHRYTELRKELLGLDDLKMYDLYTPLIKDIKFEMPYEEAKEWMLKALEP
                     MGEEYLNVVKEGLNNRWVDVYENKGKRSGGYSSGAHLTNPFILLNWSNTISDLYTLVH
                     EFGHSAHSYFSRKFQPSNSSDYTIFVAEVASTCNEALLSDYMDKHLDDEKRLLLLNQE
                     LERFRATLFRQTMFAEFEHKIHAIEEAGEPLTPTRMNEEYAKLNKLYFGDSVETDEDI
                     SKEWSRIPHFYMNYYVYQYATGYSAAQSLSHQILTEGKPAVDRYINEFLKKGSSNYPI
                     EILKNAGVDMTTPEPIEQACEVFEQKLNAFEKLMKA"
BASE COUNT         1481 a          694 c          609 g         1458 t
ORIGIN      
        1 ggtgtataca tatcatggca acaaatcatg aaatagaatt taaacaaatg ataactgcat
       61 caatctataa taaattgcaa gagaaatatt ttaaagacag tgtgcttttt aagcaagtca
      121 attactatat tgatacaccc gatttcaaat taaaggaaca tcgctctgca ctacgcataa
      181 gagttaaaga caatcaatat gaaatgactt taaagacacc agcaaaggtt ggtctattag
      241 aatacaacta tatcgtcgac attaaaccag agatgaactt gattatttca aacgacaatc
      301 ttccagatga cattcgtcaa attatagttg aacagtttgg tgtaaaagat acaactttat
      361 caatacttgg tgcattaacg acatatcgcc aagaaactaa atataaaggt gatttactcg
      421 ttttagataa aagtgagtat ttcgatactg cggattatga gttagagttc gaagttaaag
      481 actataatca aggtttacaa aagttccaat ctttattaaa cgaactaaac ctcgaacatc
      541 atcaaccttt aaataaagtt caacgttttt tcaagaaaaa agaaacactt tcaaacaata
      601 taaattaaat tggcaaatat gcatatttct tccaaatgtt taaaatcatg ttatattagt
      661 tatatattaa cttaagttcg taatagtaaa tttttgaaaa tgggtgatta aatgacaaca
      721 acaccatatg acatcattgg taaagaagcg ttatacgata tgattgatta tttttacacc
      781 cttgtagaaa aagatgaacg acttaatcac ctgtttccag gagattttgc agaaacaagt
      841 cgtaaacaaa aacaattttt aacacagttt ttgggcggtc caaacattta taccgaagaa
      901 cacggacatc ctatgctaag aaaaagacat atggatttta caatcactga atttgaaaga
      961 gatgcatggt tagaaaatat gcagacagca attaatcgcg ctgcgtttcc acaaggtgtt
     1021 ggagattatt tatttgagcg cttaagatta actgctaatc atatggtgaa ttcctaaaat
     1081 taattgtagg tgaataaaca tggctggaga attacgaata atggaaaata agagtcgtga
     1141 agatattaat ctatcacctg taagtaaaat cgaaatttat tcatttttcg atccatttag
     1201 ctccgattgc ttcaaattat cagcaatctt atccaaatta agaattgaat ataatcaata
     1261 tatacgtatc agacatatat taaatccttc gttaaaagta ttaacgaaat gccaagctca
     1321 aagtacatcc aactttgata acatcgccct agcttataaa gcagctgagt tacaaggtcg
     1381 tgtacgtgcc gaacgattta tacatttaat gcaaaatgaa atcataccta aaagagatat
     1441 tattacagaa tcaatgattt gtgactgtat tcaaaatgca ggtattgatt tagaagtatt
     1501 taaagacgac ttacaaaaaa gtaaactaac cgaaagcttg aaaattgatt tgcatattgc
     1561 aagagaaatg gaaatcgaac aagccccttc tctcgttttc tttagtgaag atgttcatga
     1621 agaaggttta aaagtcgaag gattataccc atatcacatc tatacttata taattaatga
     1681 attgatgggt aaacctatcg aaaagaatct tcctcctaaa ttagaaactt atatacagca
     1741 acaacaactt gtaacgatgg aagaattact tactatttat gaatggccag aaaaactttt
     1801 aaacaaagag ttaaagaagt tagccattca acaaaaaatt gaaaaactta aatatccaga
     1861 cggcgatttc tggaaatcta aaatgcctaa aatcaaatca aaataaataa attaaagacc
     1921 tcgtacacca taaatgtaac gaggtctttt tactgactct taattttgca acattaaatt
     1981 attagaacgt aatgttacac atcgatctaa atatattagt gatatttttc gaatttaacc
     2041 agttcgaaaa ttatacaaat tataaatttg gtacataata gacttatatt aatcgttaat
     2101 gtataaaaag tgcatattta atacattttt atacaacttc acttctctct tattaaaaaa
     2161 gaacataaaa aaacgtcgtg tctgaataca cgacgctaaa caaaggggat gggagaaatt
     2221 tttcacttca aacaaagggg tatgtttgtt atgtgattaa tttcatgttc ataatataac
     2281 atgctatctc taccatttca accttttaat agtacttaat ttaaattgtc acaatctgtt
     2341 cataatgtaa gcgaatacaa tagtactaag ctttcattaa tttttcaaaa gcgttcaatt
     2401 tttgttcaaa aacttcacaa gcttgttcaa ttggttcagg tgttgtcata tctacaccag
     2461 cattttttaa tatctcaatt ggataatttg agctaccttt tttcaagaat tcattaatat
     2521 atctatctac tgctggctta ccttctgtta aaatttgatg acttaagctt tgagctgcac
     2581 tgtaaccagt tgcgtattga tatacataat aattcatata gaagtgtgga atacgtgacc
     2641 attccttact aatatcttca tcagtttcta cagaatcacc gaagtataat ttatttaatt
     2701 tggcatattc ttcattcatt ctagttggcg ttaatggttc acctgcttct tcaattgcat
     2761 gaattttatg ctcaaattct gcgaacattg tttgtcggaa taatgtagct ctgaaacgtt
     2821 ctaattcttg gtttaataat aataagcgtt tttcatcatc aagatgttta tccatataat
     2881 cacttaaaag tgcttcgtta caagttgatg caacttcagc gacaaaaata gtgtagtcac
     2941 tagaatttga cggttggaat tttctactga agtaactatg tgctgaatgc ccaaattcat
     3001 gaactaatgt gtataagtct gaaatagtat tagaccagtt aagtagaata aatggattag
     3061 ttaaatgtgc acctgatgaa tagccacctg aacgtttacc tttattctca tagacatcga
     3121 cccaacgatt gtttaagcct tctttaacta catttaaata ttcttcaccc attggttcta
     3181 aagcttttaa catccactct ttagcttctt cataaggcat ttcaaactta atatctttaa
     3241 ttaatggtgt atataaatca tacattttca agtcatctaa acctagcaat tctttgcgca
     3301 attcagtata tctatgtagc aatggtaaat atttatgtac agtttttact agattgtcat
     3361 atacattttc tggaatatga ttattactta atgctttttc tcttgcagtt ttgtaattgt
     3421 gtgtacgagc attaaataca tttttcttca cttcacctgc tagcgtagcg ccaagcgtat
     3481 tattatgagc accatatgct ttatatacat ttctaaaagc actttctctt agtttgcgat
     3541 catctgattc taaatactta ataaatgtac cttgtgttaa cgggtgtgca tttccatctt
     3601 tatcaatcgc atcttcaaat actaaatcag cgttgctaaa cataccgtat acatttgatg
     3661 gcgttgataa cgcgtcctgc gcttctgtta ataacttttc agtttcagca tctaaaatat
     3721 gaggacgttt ttcattaatc aacttcaaat caaatgcgaa tttttgtaat ttatcatatg
     3781 aatttacaaa tgattgaatt ttatcttcat caatttgtaa aatctctggc actaagaaac
     3841 tccatgccga gctaaattta ataattaatt gatgtgctct tgactccata ccagtatact
     3901 tgtcgttcgt tgtgtcttgg tcttgtttta ggtgcgcgta tacatatact ttttctaatt
     3961 tagtacctaa tgtatcttct aattctaacg cattgtataa tgtctcagca ctatcaccaa
     4021 tgtgtccttt aaattgttct tctttgccta actcattttc aacttcttta aatgcagcct
     4081 caaaagcttc atcatctttg aaaattgttg ttaagtccca tgtatattca ggatatttac
     4141 gttcctgttc ttctctcgat aattgttgac tcatatgtag taaacctcct aataaaatat
     4201 aattgatata attttctcat tttagaacaa aatttgcacg tc
//
LOCUS       DAHHSC010000199         4202 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.200, whole genome shotgun sequence.
ACCESSION   DAHHSC010000199 DAHHSC010000000
VERSION     DAHHSC010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 4202)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4202)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4202
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.200"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            complement(81..947)
                     /gene="galU"
                     /locus_tag="NBI57_002287"
     CDS             complement(81..947)
                     /gene="galU"
                     /locus_tag="NBI57_002287"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000721336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HCD1823216.1"
                     /translation="MKKIKKAIIPAAGLGTRFLPATKAMPKEMLPILDKPTIQYIVEE
                     AARAGIEDIIIVTGRHKRAIEDHFDSQKELEMVLKEKGKSELLEKVQYSTELANIFYV
                     RQKEQKGLGHAISSARQFIGNEPFAVLLGDDIVESEVPAVKQLIDVYEETGHSVIGVQ
                     EVPEADTHRYGIIDPLTKNGRQYEVKKFVEKPAQGTAPSNLAIMGRYVLTPEIFDYLK
                     TQKEGAGNEIQLTDAIERMNNDNQVYAYDFEGERYDVGEKLGFVKTTIEYALKDDSMR
                     EELTRFIKALGL"
     gene            complement(1128..4013)
                     /gene="fnbB"
                     /locus_tag="NBI57_002288"
     CDS             complement(1128..4013)
                     /gene="fnbB"
                     /locus_tag="NBI57_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001566815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin-binding protein FnbB"
                     /protein_id="HCD1823217.1"
                     /translation="MKSNLRYGIRKHKLGAASVFLGTMIVVGMGQEKEAAASEQNNTT
                     VEESGSSATESKASETQTTTNNVNTIDETQSYSATSTEQPSKSTQVTTEEAPTTVQAP
                     KVETEMKSQEDLPSEKVADKKTTGTQVDIAQPSNVSEIKPRMKRSADVTAVSEKEVAE
                     EAKATGTDVTNKVEVTESSLEGHNKDSNIVNPHNAQRVTLKYKWKFGEGIKAGDYFDF
                     TLSDNVETHGISTLRKVPEIKSSTEDKVMANGQVINERTIRYTFTDYINNKKDLTAEL
                     NLNLFIDPTTVTKQGSQKVEVTLGQNKVSKEFDIKYLDGVKDRMGVTVNGRIDTLNKE
                     EGKFSHFAYVKPNNQSLTSVTVTGQVTSGYKQSANNPTVKVYKHIGSDELAESVYAKL
                     DDTSKFEDVTEKVNLSYTSNGGYTLNLGDLDNSKDYVIKYEGEYDQNAKDLNFRTHLS
                     GYHKYYPYYPYYPYYPVQLTWNNGVAFYSNNAKGDGKDKPNDPIIEKSEPIDLDIKSE
                     PPVEKHELTGTIEESNDSKPIDFEYHTAVEGAEGHAEGIIETEEDSIHVDFEESTHEN
                     SKHHADVVEYEEDTNPGGGQVTTESNLVEFDEESTKGIVTGAVSDHTTVEDTKEYTTE
                     SNLIELVDELPEEHGQAQGPIEEITENNHHISHSGLGTENGHGNYGVIDEIEENSHVD
                     IKSELGYEGGQNSGNQSFEEDTEEDKPKYEQGGNIVDIDFDSVPQIHGQNNGNQSFEE
                     DTEEDKPKYEQGGNIIDIDFDSVPQIHGFNKHNEIIEEDTNKDKPNYQFGGHNSVDFE
                     EDTLPKVSGQNEGQQTIEEDTTPPTPPTPEVPSEPETPTPPTPEVPSEPGEPTPPKPE
                     VPSEPETPVPPTPEVPSEPGKPVPPAKEEPKKPSKPVEQGKVVTPVIEINEKVKAVAP
                     TKQKQSKKSELPETGGEESTNKGMLFGGLFSILGLVLLRRNKKNNKA"
BASE COUNT         1054 a          818 c          656 g         1674 t
ORIGIN      
        1 cacataaatt aagcatgaca ctcattcaat ttagttcacc atttcgtgtt ccaattttat
       61 tgagtatcat gcttttaata ttataaacct aatgctttaa taaatcgtgt taattcttct
      121 cgcatactgt catctttcaa cgcatattct attgttgttt tgacaaatcc taatttttct
      181 ccaacgtcat aacgttcgcc ttcgaagtca tatgcataca cttgattatc attgttcata
      241 cgctcaatcg catctgttaa ctgaatttcg ttacctgcgc cttctttttg cgtttttaaa
      301 taatcgaaaa tttcaggcgt taatacatat cggcccataa tagctaggtt tgatggtgcc
      361 gtaccttgtg ctggcttttc aacaaacttt ttcacttcat actgacgtcc atttttagtt
      421 aatggatcaa taattccata acgatgagta tctgcttccg gaacttcttg gacacctata
      481 actgagtgcc ccgtttcttc ataaacgtca atcaactgtt tcactgctgg cacttcagat
      541 tcaacaatat cgtcacctaa taataccgca aatggttcat tgccgataaa ttgacgcgca
      601 gaactaatcg catgccctaa acctttttgt tctttctgtc ttacataaaa aatattcgca
      661 agttctgtcg agtattgaac tttttctagt aattcagatt tacctttttc ttttaacacc
      721 atttctaatt ctttttgact atcaaaatga tcttcaatcg cgcgtttgtg gcgacctgtc
      781 actataataa tatcttcaat tccagctctt gcagcttctt caacgatata ttgtattgtg
      841 ggtttatcta agataggaag catttccttt ggcatcgctt tagttgctgg taaaaatcta
      901 gtccctaaac cagcagcggg aatgattgcc ttttttattt ttttcaaagt taatgtgctc
      961 cttttcctaa gtattaaatc tatgtatcaa cgtcatttta acactaatta gaacgccttc
     1021 atagtgtcat tgagtatgta attatttctt gggaaatttg atttaatttt aaaaaaacag
     1081 gtttacttca tataatttat gaaataaacc cgtcaatttt tgtttaatta tgctttgtta
     1141 ttctttttat ttctgcgtaa taatactaaa cctagaatgc tgaataatcc gccgaacaac
     1201 atacctttgt ttgttgattc ttctccacct gtttcaggta gttcagattt cttagattgt
     1261 ttttgtttag ttggtgccac tgctttaacc ttttcattga tttcaataac aggtgttact
     1321 accttacctt gttccactgg tttagaaggt tttttaggtt cttctttagc aggtggtact
     1381 ggtttaccag gttcagatgg tacctctggt gttggtggta ctggtgtttc cggctcactt
     1441 ggtacttccg gttttggtgg cgttggttcg cctggctcac tcggtacttc tggtgttggt
     1501 ggtgttggtg tttccggctc acttggtacc tctggtgttg gcggtgttgg cggcgttgta
     1561 tcttcttcaa tcgtttgttg accttcattt tgaccactta cttttggaag tgtatcttct
     1621 tcaaaatcaa cactgttgtg tccaccaaat tgataattag gtttatcttt gtttgtatct
     1681 tcttcaataa tttcattatg cttattgaat ccatgaattt gtggcacact gtcgaagtcg
     1741 atatcaatga tgttaccacc ttgttcatac ttaggcttgt cttcttctgt gtcttcctcg
     1801 aatgactggt taccattatt ttgaccatga atttgaggta cactgtcgaa atcgatatct
     1861 acgatattac caccttgttc atatttaggt ttatcttctt ctgtgtcttc ctcgaatgac
     1921 tgattaccgc tattttggcc accttcataa cctaattcac tcttaatatc aacgtggcta
     1981 ttttcttcga tttcatcaat cacgccataa ttaccgtgac cattttcagt tcctaaacca
     2041 gaatgagaaa tatgatgatt gttttcagta atttcctcga ttggcccttg tgcttgacca
     2101 tgttcttcag gtaattcatc cactaattca atcagattac tttcagttgt atattctttc
     2161 gtatcttcaa ctgttgtatg gtcgctcact gcgccagtta caataccttt tgtagactct
     2221 tcgtcaaatt caactaagtt agactcagtt gttacttggc caccacctgg gtttgtatcc
     2281 tcttcatatt caacaacatc agcgtgatgt tttgaatttt catgtgtaga ttcttcaaaa
     2341 tccacatgaa tagaatcttc ttcagtttca ataatacctt ctgcatgacc ttctgcacct
     2401 tcaacagctg tatgatattc aaaatcaatt ggcttagaat cgttactttc ttcgattgta
     2461 ccagtcaatt catgcttctc cactggtggc tctgatttaa tgtctaaatc aattggttca
     2521 ctcttctcaa tgataggatc atttggttta tctttaccat cgcctttagc attattagag
     2581 taaaatgcaa caccgttgtt ccaagttaat tgaactggat aatacgggta ataaggatag
     2641 tatgggtagt atttatgata tcctgaaaga tgtgttcgga aatttagatc cttagcattt
     2701 tgatcatatt caccttcata tttaattaca tagtctttcg aattatctaa atcgccaagg
     2761 ttcaatgtgt acccaccatt acttgtgtaa gatagattta ctttttcagt cacatcttca
     2821 aatttactgg tatcatcaag ctttgcataa acactttcag ctaattcatc tgaaccaatg
     2881 tgtttatata ctttgactgt tggattatta gcactttgtt tatatccaga tgttacttga
     2941 ccagttactg tgacagaagt taacgactgg ttgttaggct tcacatatgc aaaatggcta
     3001 aatttaccct cttctttatt caaagtatca atacgaccat taacagtaac acccattcta
     3061 tctttaacgc cgtctaaata tttgatatca aattcttttg agactttatt ttgacctagt
     3121 gttacttcaa ctttttgact cccttgcttt gtcactgttg ttgggtcaat gaataggttt
     3181 aagtttaatt cagcagttaa atcttttttg ttatttatat aatcagtaaa tgtatagcga
     3241 attgtacgtt catttataac ttgaccattt gccataactt tatcttctgt tgaacttttt
     3301 atctccggaa ctttacgcag tgttgatata ccatgtgttt caacattatc acttaatgtg
     3361 aaatcaaaat aatctcctgc cttaattcct tctccaaatt tccatttgta ttttaaagtt
     3421 actctttgag cattatgcgg attaacaata ttcgaatctt tattatgtcc ttctaaagag
     3481 ctttcagtaa cttccacttt atttgttaca tctgtacctg tcgctttagc ttcttccgct
     3541 acttctttct ctgaaactgc tgtaacgtca gctgatcttt tcattcttgg tttaatttct
     3601 gagacgttac ttggttgagc tatgtcaact tgagttcctg tagttttctt atcagcaact
     3661 ttttctgatg gtaaatcttc ttgtgatttc atttcggttt ctacttttgg tgcttgcaca
     3721 gttgttggtg cttcttctgt tgttacttga gttgattttg atggttgctc agttgatgtc
     3781 gcgctgtatg attgtgtttc atctattgta ttaacgttat ttgtagttgt ttgtgtttcg
     3841 cttgctttac tttcagtagc tgaactccca ctttcctcta ctgtagtatt gttttgttcc
     3901 gatgctgcag cttctttttc ttgtcccatt ccaacaacga tcattgttcc taagaatact
     3961 gaggccgctc ccaatttgtg ttttcttatg ccgtatctaa gattgctttt cactataata
     4021 ttctccctta aatgcaaaat tcatttattt ttaaaactca ataaatgcaa ttctatattg
     4081 ttcggttttt aaaagcaatg aaaaaaagcg agttaataaa aagttaagat tgttgttaac
     4141 tttatgtata atgagttttt tattatttat aactcacata tatattgcat gcaaaactct
     4201 tg
//
LOCUS       DAHHSC010000200         4153 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.201, whole genome shotgun sequence.
ACCESSION   DAHHSC010000200 DAHHSC010000000
VERSION     DAHHSC010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 4153)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4153)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4153
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.201"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            100..1194
                     /locus_tag="NBI57_002289"
     CDS             100..1194
                     /locus_tag="NBI57_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014667727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HCD1823218.1"
                     /translation="MRLGARIFKTGIAIILAMSIASLLPDDVGLKALAGVSAVVAMQP
                     SIYRSFKTVSDQALGNIIGAILSVTMVTIFSDNFIIMGVTVIVLIAILFKFNLAHVAT
                     LASVTALIIMGQHTGSFYITAFYRFVLVMIGVISSSLVNFVFLPPKFETKIYYNSLNI
                     SSDIFMWFKLVLNDTTEFNNIKQDSHNLKQRVEKLEKIYDYYSEERPITKKHIHQQNR
                     KKILFREVVQTTRQAYEVLNKLSRYQNDLYLLNNNFLLQIKLDLDSLTAFHEQILASL
                     SKKARYNVTHVDYELDNPQKKDLLSTFQHELINHPYQTEYSFANVMQIVAAIEEYRHH
                     LEHLDRIRISFFTYHRSDADIEIVEEDFDL"
     gene            complement(1284..1375)
                     /locus_tag="NBI57_002290"
                     /pseudo
     CDS             complement(1284..1375)
                     /locus_tag="NBI57_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500499.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1384..3120)
                     /locus_tag="NBI57_002291"
     CDS             complement(1384..3120)
                     /locus_tag="NBI57_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAV1866 family putative multidrug efflux ABC
                     transporter"
                     /protein_id="HCD1823219.1"
                     /translation="MIKRYLQFVKPYKYRIFATIIVGIIKFGIPMLIPLLIKYAIDGV
                     INNHALTTDEKVHHLTIAIGIALFIFVIVRPPIEFIRQYLAQWTSNKILYDIRKKLYN
                     HLQALSARFYANNQVGQVISRVINDVEQTKDFILTGLMNIWLDCITIIIALSIMFFLD
                     VKLTLAALFIFPFYILTVYVFFGRLRKLTRERSQALAEVQGFLHERVQGISVVKSFAI
                     EDNEAKNFDKKNTNFLTRALKHTRWNAYSFAAINTVTDIGPIIVIGVGAYLAISGSIT
                     VGTLAAFVGYLELLFGPLRRLVASFTTLTQSFASMDRVFQLIDEDYDIKNGVGAQPIE
                     IKQGRIDIDHVSFQYNDNEAPILKDINLSIEKGETVAFVGMSGGGKSTLINLIPRFYD
                     VTSGQILIDGHNIKDFLTGSLRNQIGLVQQDNILFSDTVKENILLGRPTATDEEVVEA
                     AKMANAHDFIMNLPQGYDTEVGERGVKLSGGQKQRLSIARIFLNNPPILILDEATSAL
                     DLESESIIQEALDVLSKDRTTLIVAHRLSTITHADKIVVIENGHIVETGTHRELIAKQ
                     GAYEHLYSIQNL"
     gene            complement(3411..3953)
                     /locus_tag="NBI57_002292"
     CDS             complement(3411..3953)
                     /locus_tag="NBI57_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HCD1823220.1"
                     /translation="MVRESIPKEGENIKIQSYKHDGKIHRVWSETTILKGTDHVVIGG
                     NDHTLVTESDGRTWITREPAIVYFHSEYWFNVICMFREDGIYYYCNLSSPFVCDEEAL
                     KYIDYDLDIKVYPNGKYHLLDEDEYEQHMNQMNYPHDIDIILRRNVDILQQWIEQKKG
                     PFAPDFIKVWKERYKKIRQY"
BASE COUNT         1397 a          730 c          570 g         1456 t
ORIGIN      
        1 caactataaa taagcttgaa ttttataact agtaagtgta taacagtaat gaatacgaat
       61 attagatatt caataaactt aaaaggggcg tcattatttt tgagactagg agctcggatt
      121 tttaaaactg gtatagccat tattttagct atgtctatcg cttctttact accggatgat
      181 gttggtctga aagccttagc tggtgtcagt gctgttgttg caatgcaacc tagtatttat
      241 cgttcattta aaacagtttc tgatcaagca ttgggtaaca ttataggtgc catattatct
      301 gttacaatgg taacaatttt tagtgataat tttatcatta tgggtgttac cgtcatagta
      361 cttattgcta ttttatttaa atttaattta gcacatgttg ctactttggc gagtgtaaca
      421 gcattaatca ttatgggaca acataccggt tctttctata ttaccgcctt ttacaggttt
      481 gtacttgtaa tgattggtgt cataagttca tctctagtta actttgtgtt tctaccacct
      541 aaatttgaaa caaaaattta ctataattca ttaaatatat cctcagatat ttttatgtgg
      601 tttaaattag tattaaatga tacgacggag tttaataata ttaaacaaga tagtcataat
      661 ttaaaacaac gtgtcgaaaa gttagagaaa atttatgatt actatagcga agaacgtcct
      721 attacaaaaa aacatattca ccaacagaat agaaagaaaa tacttttcag agaagtagtt
      781 cagacgacta gacaagctta tgaagtatta aacaaattgt cgcgttatca aaacgactta
      841 tatttactta ataacaattt cctattacaa atcaaattag atttagattc attaactgct
      901 tttcacgagc aaattttagc tagcctttct aaaaaagcac gttataatgt gacacatgtt
      961 gattatgagt tagataatcc gcagaaaaaa gatttattgt ctacatttca gcatgagtta
     1021 ataaatcatc cttatcaaac ggaatattcg tttgccaatg ttatgcaaat tgttgcagcc
     1081 atcgaagagt atcgacacca tttagagcat ttagaccgca taagaattag ctttttcaca
     1141 tatcataggt ctgatgcaga cattgaaatt gttgaagaag actttgattt ataagtttaa
     1201 agcaattcct gtaattaata ccacaatgag ataaagtaat attgaatctc gaaattaaat
     1261 ttaaaaaagc agtaagatta ttttcaatga agaaaatctc ttactgcttt tctatgttat
     1321 gaccccacca tcctgttaca aatcttccct acaagattgt actgaagtca aacaactaat
     1381 actttataag ttttgaatgc tatataaatg ctcgtaagca ccttgttttg caatcaattc
     1441 acgatgcgta cctgtttcaa caatatgtcc attttcaatt acgacaattt tgtcagcatg
     1501 tgtaatagtg gacaagcgat gcgctacgat aagtgtcgtt cgatctttac tcaacacatc
     1561 taatgcttct tgaataatgg attcactttc taaatcaagt gcacttgttg cttcatccaa
     1621 gataagaatt ggcggattat ttaaaaatat tctagcaatc gataatcttt gtttttgacc
     1681 acctgataat ttaacacctc gttcacctac ttcagtgtca tatccctgtg gcaagttcat
     1741 aataaagtca tgtgcattag ccattttcgc cgcttcaact acttcttcat ctgttgctgt
     1801 tggacgacca agtaaaatat tttccttaac tgtatcagag aataaaatat tatcctgttg
     1861 caccaatcct atttgatttc ttaaacttcc cgttaaaaaa tctttaatat tgtgaccatc
     1921 tattaaaatt tgcccagaag ttacatcgta aaatctcggt attaagttaa ttaatgttga
     1981 tttaccacca ccactcatac ctacgaaagc aactgtttct cctttttcaa tactcaaatt
     2041 aatatctttt aaaattggag cttcgttatc gttatattga aaactaacat gatcaatatc
     2101 aatacgacct tgtttaattt caataggttg agcaccaaca ccatttttaa tgtcataatc
     2161 ttcgtcaatt aattggaata cacggtccat tgaagcaaaa ctttgcgtta aagttgtaaa
     2221 tgatgcgact aaacgacgta aaggcccgaa caataactct aagtatccaa caaatgctgc
     2281 aagtgtacct actgtgattg atccagaaat agcaagatat gcaccaacac cgatgacaat
     2341 aattggtcca atatctgtaa ctgtattaat tgcggcaaag gaataggcat tccatcttgt
     2401 atgtttcaac gcacgtgtta agaaattagt attcttttta tcaaagtttt tcgcttcatt
     2461 gtcttcaatc gcaaaacttt taacgactga aataccttga acacgttcat gcaagaatcc
     2521 ttgaacctca gctaatgctt gcgatctctc acgcgtcaat tttcttaatc ttccaaagaa
     2581 aacgtacacc gttaaaatgt aaaatgggaa gataaacagt gctgctaaag tcaatttcac
     2641 atctaaaaag aacattatgg atagtgcaat aataattgtt atacaatcta accaaatatt
     2701 cattaacccg gttaaaatga aatcttttgt ttgttcaaca tcattaatca ctctagatat
     2761 tacttgacct acttgattat tagcataaaa tctcgcactt aaagcttgta aatggttata
     2821 taacttttta cgtatatcat acaatatttt attacttgtc cattgcgcca aatattgacg
     2881 tataaattca attggtggtc tcactattac aaaaataaat aatgcgatac caatggcaat
     2941 agttaaatga tgaacttttt catcagtcgt tagtgcgtgg ttattaatca cgccatctat
     3001 tgcatattta attaaaagtg gtataagcat tggtatacca aacttaatta tcccaacaat
     3061 aatcgttgca aaaatacgat atttatatgg cttaacaaat tgcaaatatc gtttaatcat
     3121 acaatccccc ctaatctatt gccctatcct attcataagc ataaaaatga atagaggttg
     3181 gatacataat ttgtagatgt aaattcttct tacaatttac atttttaaaa ttaagatatc
     3241 tcaacctctg tcatattcat gtgattaaat acgttgtctt attcgtatgt tatattctat
     3301 tcaatttaat ctatggatac tgtgtcccca cacgacagca aaagttatca tacttcttta
     3361 catcactaag tcaatataaa tgatttaatc agtatttaca ctttatttgc ttaatactgt
     3421 ctaatttttt tgtaacgttc tttccaaact ttgataaaat ctggcgcgaa tgggcccttc
     3481 ttctgttcta tccattgttg aagaatgtcc acgttgcgtc ttaaaataat atcaatatca
     3541 tgcggataat tcatttgatt catatgttgc tcatattcat cttcatctaa taaatgatac
     3601 tttccgtttg gatatacttt aatatctaaa tcatagtcta tatattttaa tgcctcttca
     3661 tcacaaacaa atggtgatga caaattgcaa tagtaataaa ttccatcttc tctaaacatg
     3721 cagataacat taaaccaata ttctgagtga aagtaaacaa ttgccggttc acgtgttatc
     3781 caagttcttc cgtcactttc agtcactaac gtatgatcat tgccaccaat gacaacatga
     3841 tcagtaccct ttaatattgt tgtttcagac caaacgcgat gaatcttacc atcatgttta
     3901 taactctgaa ttttaatgtt ttccccttct ttaggtatgg attctctgac catactccac
     3961 accaccttct gttaatttaa ccattataaa ttatagcata tttcagaaat agtattatat
     4021 aaatacatat ttttacgaaa taagatttta ctacttaatg attaaactcg gtaatattgc
     4081 taagtactac aacagagatt tacatgtccc atttaaagta tgtaaaatca tcacttttat
     4141 aaatcaacac ttt
//
LOCUS       DAHHSC010000201         4136 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.202, whole genome shotgun sequence.
ACCESSION   DAHHSC010000201 DAHHSC010000000
VERSION     DAHHSC010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 4136)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4136)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4136
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.202"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            421..1569
                     /locus_tag="NBI57_002293"
     CDS             421..1569
                     /locus_tag="NBI57_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001806960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC family ATPase"
                     /protein_id="HCD1823221.1"
                     /translation="MRLNKNESELKLCRDLITEFETLSKYNNITNFEVDYKKYVQDVN
                     QHQELSKEIEDKLMQLSQRKLIEQNNLNHYENQLETYNNDLELNEQSIEMEMSRLNLT
                     DDNDINEIIAWRGEQEELEQKRDTYKKRYHEFEMEIARLESLTKDKELLDSDKLKDEY
                     EQKKEKMNTLIDEYSAVHYQCQNNINKTQSIVSHINYLNQELKDQQEIFQLAEIVSGK
                     NNKNLTLENFVLIYYLDQIIAQANLRLATMSDNRYQLIRREAVSHGLSGLEIDVFDLH
                     SNKSRHISSLSGGETFQSSLALALGLSEIVQQQSGGISLESIFIDEGFGTLDQETLET
                     ALDTLLNLKSTGRMVGIISHVSELKNRIPLVLEVKSDQYQSSTRFKRN"
     gene            complement(1640..2002)
                     /gene="mscL"
                     /locus_tag="NBI57_002294"
     CDS             complement(1640..2002)
                     /gene="mscL"
                     /locus_tag="NBI57_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008530241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HCD1823222.1"
                     /translation="MLKEFKEFALKGNVLDLAIAVVMGAAFNKIISSLVENIIMPLIG
                     KIFGSVDFAKEWSFWGIKYGLFIQSVIDFIIIAFALFIFVKIANTLMKKEEAEEEAVV
                     EENVVLLTEIRDLLREKK"
     gene            2206..3852
                     /locus_tag="NBI57_002295"
     CDS             2206..3852
                     /locus_tag="NBI57_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BCCT family transporter"
                     /protein_id="HCD1823223.1"
                     /translation="MNSSSPENPNGKKYSPVFIYSAIVVAIVVLLGAFLPEQFNYVTN
                     NIKMWITEKLGWYYLILTTIIVFFCIFLIFSPIGKLKLGKPNDKPEFNTISWFAMLFS
                     AGMGIGLVFYGAAEPMAHFATPPTADPKTTEAYTEALRSTFFHWGFHAWAVYGVVALA
                     LAYSQFRKGEPGLLSRTLRPLLGDKVEGPIGIFIDVLSVFATIVGVAVSLGMGALQIN
                     GGLHYLFNVPNNTFVQAIIIIVVTILFIASAWSGLSKGIQYLSNLNIGLGTILMVAAL
                     IVGPTVLILNMLTSSTGSLLNTFLFNSFDTAALNPQKREWMSSWTLYYWGWWLSWSPF
                     VGVFIARVSKGRSIREFISGVLLVPAIVSFVWFSVFGVLGIETGKKHKEIFDMTPETQ
                     LFGVFNHVPFGIVLSLIALLLIASFFITSADSATFVLGMQTTFGSLNPSSMVKVVWGI
                     SQALIAFVLLLAGGGNGAEALNAIQSAAIISAFPFSFVVILMMVSFYKDANQERKFLG
                     LTLTPNKHRLQEYIKSQQEDYESDILEKRQSRRNIEKKDN"
BASE COUNT         1459 a          584 c          724 g         1369 t
ORIGIN      
        1 ttagatcact acgataaatc aaaattggac ttaaatgata aagaaagctt tataagtgaa
       61 attaaatctg ctgtaaagat tggagatcaa tgtccgatat gtggtaatga aattcaggat
      121 ttagggcatc atattgattt tgacagtatt gctaaacgtc aaaatgaaat taaagaaatt
      181 gaagcaaata tccacgcaat aaaatcgaat attgctgtgc ataattctga aattaaattt
      241 gttaatgaaa aaatatcgaa tattaatatt aaaacgcaaa gtgatttttc actcgaagta
      301 ttgaataagc gtctgctaga aaatgaaaat gcattgaata atcaaagaga tcttaataaa
      361 tttatagaac aaatgaaaga agaaaaagat aatctaacgt tgcaaattca taataaacaa
      421 ttgcgtctaa ataaaaatga atctgagttg aaattatgtc gagatctcat cactgaattt
      481 gaaacactct cgaaatataa taatatcact aattttgagg tggattataa gaagtatgtt
      541 caagatgtga atcagcatca agaactctca aaggagattg aagataagtt aatgcaattg
      601 tctcaaagaa agttaattga gcaaaataat ctaaatcact atgaaaatca actagaaact
      661 tacaataatg acttagaatt gaatgaacaa tctattgaaa tggaaatgtc gaggctgaat
      721 ttaactgacg acaatgatat aaatgaaata atagcctgga ggggcgagca agaggaatta
      781 gagcagaaaa gggatactta taaaaaacgt tatcatgaat ttgaaatgga aatagctagg
      841 ttagaatcat taaccaagga taaagagtta ttggactctg ataaattaaa agatgaatat
      901 gagcaaaaaa aagaaaagat gaatacactg atagatgaat actctgctgt tcattatcaa
      961 tgtcaaaata atattaataa aacacaatct atagtttcgc atattaatta cttaaatcaa
     1021 gaattaaagg atcaacaaga aatttttcaa ttggctgaaa ttgtcagtgg taagaataac
     1081 aaaaatctta cattggaaaa ctttgtctta atttactatt tagatcaaat tattgcccaa
     1141 gcaaatctga gattagcaac aatgtcagat aatcgatacc aactaattag gcgagaagcg
     1201 gtttctcatg gtcttagtgg cctagaaatt gatgtatttg atttgcattc aaataagtct
     1261 agacatatta gctcgttatc aggtggagaa actttccaat cgtcgcttgc attagcttta
     1321 gggttaagcg aaattgtaca gcagcaatca ggaggtattt cactagaatc aatatttatt
     1381 gatgaaggat tcggtacatt agatcaagaa acgcttgaaa cagcgttaga cactttatta
     1441 aatcttaaat caactggtag aatggttggg attatttcac atgtgagcga attgaaaaat
     1501 agaatacctt tagttttaga agtgaaatca gatcaatatc agagttcaac aagattcaaa
     1561 agaaattaaa acataaaaaa tagagatggt actttggaat gtatagaaca accttagccc
     1621 atctcttttt atttaaaaat tattttttct cacgcaataa atctctgatt tcagttaata
     1681 acacaacatt ttcttccaca actgcttctt cttcggcttc ttctttcttc attaatgtat
     1741 ttgcaatctt aacaaagatg aataaagcaa acgcgatgat aataaagtcg ataacagatt
     1801 ggataaataa accgtattta ataccccaga atgaccattc tttagcaaaa tcaactgatc
     1861 cgaaaatttt accaattaat ggcatgatga tattttctac taatgaagat ataatcttgt
     1921 tgaaagctgc acccatcaca acagcaattg ctaaatctaa gacgttacct tttaaggcga
     1981 actctttgaa ttcttttaac attacactca acctctcttt tttaaatgta acctcaatta
     2041 tacaacaaat aaaaaaatta gaaaatagat tattcaagaa aatttgatta tcgcaaagac
     2101 ctttgttatc ctataaaggt gcgtgagtta ttgaataaaa tgtttagata atctttttat
     2161 tctactacat aaaattaaat atagaattga agggagtaat taattatgaa ttcttcttca
     2221 ccagagaatc caaatggaaa gaagtattca ccagtcttca tctatagtgc aattgttgtt
     2281 gctatagtcg tattacttgg tgcattttta cctgaacaat tcaactatgt taccaataat
     2341 attaaaatgt ggattacaga aaagttaggt tggtattatc ttattcttac tacgattatc
     2401 gtgttcttct gtatattcct tatttttagt cctattggaa aacttaaact aggtaaacca
     2461 aatgacaaac ctgagtttaa tacaatttca tggtttgcta tgttgtttag tgctggtatg
     2521 gggataggtt tggtgtttta tggtgcagct gaaccgatgg cgcactttgc tacgccacct
     2581 acagcagatc ccaaaactac tgaagcttat actgaagctc tacgttcaac atttttccat
     2641 tggggattcc atgcttgggc tgtttatggt gttgttgcgt tagcgttggc atattcgcaa
     2701 ttccgtaaag gtgaaccagg tttattatct agaactttac gtcctctttt aggtgataaa
     2761 gtagaaggtc ctattgggat ttttattgac gttttatctg tatttgcgac aatcgttggg
     2821 gtagccgttt cgttaggtat gggtgctcta caaattaatg gtggtttaca ttacttgttc
     2881 aatgttccaa acaatacgtt tgtacaagcg attatcatca ttgttgttac tatcttattt
     2941 atagcaagtg catggtctgg attaagtaaa ggtattcaat acttaagtaa cttgaacatt
     3001 ggtttaggta ctattttaat ggtagctgct ttaattgttg gaccaactgt tcttatttta
     3061 aatatgttaa ctagctctac gggtagttta ctaaacacat tcttgtttaa tagttttgat
     3121 acagctgctt taaatcctca aaaacgtgaa tggatgtctt catggacact ttattactgg
     3181 ggttggtggt taagttggag cccattcgtt ggagtgttta ttgcacgagt ttcaaaagga
     3241 cgttcaatta gagagttcat ttctggtgtc ttgctagttc cagcaattgt tagttttgtt
     3301 tggtttagtg tctttggtgt attaggcatc gagacaggta agaaacacaa agaaattttt
     3361 gatatgactc ctgaaacaca gctatttgga gtgtttaatc atgtgccatt tggcatagtt
     3421 ttatcgttga ttgcattatt attaattgca tcattcttta ttacatctgc tgactcagca
     3481 acatttgtat taggaatgca aacaacattt ggttcattaa atccatctag tatggtaaaa
     3541 gttgtttggg gaatttcaca ggccttaata gcatttgtac ttttattagc tggtggcggt
     3601 aatggcgctg aagctttaaa tgcgattcaa agtgctgcaa ttataagtgc attcccattc
     3661 tcctttgtcg tcatactcat gatggtaagt ttctacaagg atgcgaacca ggaacgtaaa
     3721 ttcctaggtt taacattgac tccgaataaa catcgcttac aagaatatat caagagtcaa
     3781 caagaagatt atgaatctga cattcttgaa aagcgtcagt cacgtagaaa tatagagaaa
     3841 aaagataact aataaaattt agttaagtat ttaaataaca agtagtatgt tattctagta
     3901 gctagaacag atatactact tgtttgtttt tgtggaaaat tgagtatatt caaaaggata
     3961 agaacgagaa tttcgttatt aaactacgaa ttctcgattt ttttatattt taagataggt
     4021 ttatttctga caacttaata gaaagaggtt tgacaaagct aaagtgaagt acgacggtat
     4081 aaaaaaatta actatacttt tatagaaaat taactcagac tgggacatag atcaat
//
LOCUS       DAHHSC010000202         4131 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.203, whole genome shotgun sequence.
ACCESSION   DAHHSC010000202 DAHHSC010000000
VERSION     DAHHSC010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 4131)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4131)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4131
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.203"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            <1..1131
                     /locus_tag="NBI57_002296"
     CDS             <1..1131
                     /locus_tag="NBI57_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001251209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsK"
                     /protein_id="HCD1823224.1"
                     /translation="VICKLIAQLLIRFKTKDLAEMIETQGFHNRTVIKKTTEYIPIDY
                     KQTEYDYYPTMFYKRGKKTFVIHIKKDGSRFQDNYLELETIIEPMFNSELVEKKHIGR
                     YLRYEFMPLKYKKRIVMNGEQESNTIFYDTKIAITHQITWDFVKAPHGLVTGITGGGK
                     TYFLFYVIRELFRRHSEVRLLDPKVSDLSFMKRVIGDDKVADTKGQILKQLREANNEM
                     EERFRLMNDSSDYKIGNDFRNFDMRPYFIIFDEVTAFTSTLDKKELQEMNDYLINIIM
                     KGRQAGVFMFLTAQRPDADVIKGNVRDQLGLRVSLGNLSNDGYRMTFGQTDKEFQTIH
                     DSDIGRGYITILGQYNEPILFDAPLMEQYDFVEDVKQILNKE"
     gene            1136..2983
                     /locus_tag="NBI57_002297"
     CDS             1136..2983
                     /locus_tag="NBI57_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000681156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1823225.1"
                     /translation="MKHKSVYLLCFLILPILLLTSVSAYAVSNPVGEPSHAVKPKIEK
                     YDLSHYRSIYSEKGDWNPFGQEEISRQINNVSDFFFSMTKILAGVTDYAIENLFQLDV
                     INDFADKIGDFVGDIYQKLLSNLAITLFIIVCFNAFIIFSVQGNAREALKRAFLIMCL
                     IGFGVGILANAGNIIRGTNNIGKDLNNIIMNSTSSIDGNIDYIHENSGMNHIRNQLFD
                     MTIYRTYLVMNYGTVDEKEIKAKGKDRIDNILKQDFSKKGQDEIDKIVKNEVEKKDNK
                     YMTQGYVFQKLAISVIGFIITVFMSIVFLSISFAKLIFSTFALFLFLFLVFSWIVSFI
                     PGFELSVFSAFAKTLGYIILSACMTFLFVIVGLCIKLANSFIEPDSQNAYFLNSIFII
                     VILFVMYKKRAQIINFVSRGNISFSPSAIGAGVMNRTQERFNKIRHEQAQNKKAKREN
                     QRDEPAPPLQNDNDLRRRQQDKPMPLFINKDNQKNGNKRREQQESMNGNDVKSASVES
                     NANDYSKQPQKASQQEHQVRETRQRKDIQRSPQVVNQPLNNENHSINRKEQKSVQTAY
                     DTDVQKRQIQNATQNQQSRQSGNRNQPITRNSQSKDRLKEQKDINKHGK"
     gene            2973..4019
                     /locus_tag="NBI57_002298"
     CDS             2973..4019
                     /locus_tag="NBI57_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000247473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HCD1823226.1"
                     /translation="MVNKKDIAKKVIGKTPIGVKLKLAKVIIILCVVAFFTFPVIVMF
                     LLAPDLNKGKDDAGCTVSGGNVSANGIDKFNENAKGGKLEGKGKEIQKIAEKNKVPVN
                     IFMAIIASESQWGKGENATRQNNPLSVMGSKSIHDSTYPTIEDGLNAGAKNLYDVYIS
                     KGLDTPKKIGPKYAPVGASNDPNNMNARWIPTVEKIMKDLGGSEAKTSCSNGKGKSIK
                     FNGKLPHWSNDDPGKGNLYTAGQCTWYAYGMRQKMGKPVSTYWHDAHKWNDRAKAEGY
                     KVDKNPEPGALFIAEQGAGGHDGHYGHVAVVIGVSDGGKTFRITEMNWEGAFKVNERT
                     LKMTDGYSFIHDKE"
BASE COUNT         1498 a          493 c          787 g         1353 t
ORIGIN      
        1 gttatatgta agcttatcgc tcagctttta attcgtttta aaacaaaaga tttagctgaa
       61 atgattgaaa cacaaggctt tcataatcgt acagtaatca agaaaacaac cgagtatatc
      121 cctattgatt ataaacaaac tgaatatgac tattatccaa cgatgtttta taaacgtggt
      181 aaaaaaactt ttgttattca tattaaaaaa gacggttcga ggtttcaaga taattattta
      241 gagttagaaa cgattattga accaatgttt aattctgaat tagtagaaaa aaagcatatt
      301 ggtcgttatt tgagatatga atttatgcca cttaaatata aaaagcgtat tgttatgaat
      361 ggcgaacaag aatcaaatac aattttttat gatacaaaaa ttgctattac gcatcaaatt
      421 acttgggatt ttgtgaaagc acctcatgga ttggttacag gtataaccgg aggtggtaaa
      481 acatattttt tattttatgt gattcgtgag ttattccgta gacattcaga agtacgttta
      541 ttagacccaa aagtttcaga tttaagcttt atgaagcgcg ttattggtga tgataaagtt
      601 gcagatacta aaggtcaaat attaaaacaa ttgagagaag ctaataatga aatggaagag
      661 cgttttagat taatgaatga tagttcggat tataaaatcg gaaatgattt ccgtaatttt
      721 gatatgcgcc cctatttcat tatttttgat gaggtaacgg cttttacttc aactttagat
      781 aaaaaagaat tacaagaaat gaatgattat ttaattaata ttattatgaa aggtcgtcaa
      841 gctggtgtat ttatgttttt aacagcacaa agaccagatg cagatgtgat taaaggtaat
      901 gtgcgtgacc aattagggtt acgtgtgtct ttaggtaatt tgtcaaatga tggttatcgt
      961 atgacctttg gtcaaactga taaagaattt caaacgattc atgatagtga tatcggccga
     1021 ggttatataa ctatacttgg acaatataat gaaccaattt tatttgatgc acctttaatg
     1081 gaacaatatg attttgtgga agatgtgaaa caaatattaa ataaggagtg atttgatgaa
     1141 gcataaaagt gtgtatcttt tatgcttttt aattttgcct attctgcttt taacatctgt
     1201 gagtgcttac gctgtttcta atccagttgg agagccctca catgcagtta agcccaaaat
     1261 tgaaaagtat gatttatcac attatcgttc aatatatagt gaaaaaggcg actggaatcc
     1321 gtttggtcaa gaagaaattt cacgacaaat taataatgtg tcagattttt tcttttctat
     1381 gactaagatt ttagcaggtg ttacagatta tgcgattgaa aatttatttc aactagatgt
     1441 tattaatgat tttgcggata aaatcggcga ttttgttggc gatatatatc aaaagttatt
     1501 gagtaatttg gcaataacat tatttattat tgtttgtttt aacgcattta ttatttttag
     1561 tgtacaaggt aatgcaagag aagcattaaa gcgagcgttt ttaattatgt gtttgattgg
     1621 ttttggtgtt ggtatacttg cgaatgcagg taatattata cgtggtacaa acaatattgg
     1681 taaggatttg aataatataa ttatgaatag tacatcttct attgatggga acattgatta
     1741 tattcatgag aattcaggta tgaatcatat acgtaatcaa ttatttgata tgacgatata
     1801 tagaacatat ttagtaatga attatggaac tgtagacgaa aaagagataa aagcaaaagg
     1861 taaagaccgt attgataaca ttttaaaaca agatttttca aaaaaaggac aagatgagat
     1921 tgataaaatt gtaaaaaatg aggttgagaa aaaagataat aaatatatga cgcaaggtta
     1981 tgtatttcaa aagttggcta tttcagttat tggctttatt attactgttt ttatgtcaat
     2041 tgtattttta tcaattagtt ttgcgaagtt gattttttct acatttgctt tatttttatt
     2101 cttattttta gtattcagtt ggatagtgag ttttatacca ggatttgaat tatctgtttt
     2161 ctctgcgttt gcaaaaacgt taggctatat tattttaagt gcctgtatga cattcttatt
     2221 tgtgatagtt ggtttgtgta tcaagcttgc aaatagtttt attgaaccgg atagtcaaaa
     2281 tgcttatttt ttaaattcaa tatttataat tgtaatattg tttgtaatgt ataaaaaacg
     2341 ggctcaaatt atcaattttg taagtcgtgg taatattagt ttttctccta gtgcaattgg
     2401 tgctggtgtg atgaatcgaa cacaagaacg ttttaataag attcgtcatg aacaagcgca
     2461 aaataagaaa gctaaacgag aaaatcaaag agatgaaccc gccccaccac ttcaaaatga
     2521 taatgattta agaagaagac agcaggataa accaatgcct ttatttataa ataaagataa
     2581 tcaaaaaaat ggtaataagc gaagagaaca acaagagtca atgaatggta atgatgtaaa
     2641 atctgcttct gttgaatcaa atgcaaatga ttattctaaa caaccacaaa aagcatcaca
     2701 gcaagagcat caagtacgag aaacaagaca gcgaaaagat atacaaaggt cgccacaagt
     2761 ggttaatcag ccattaaata atgagaatca ttcaataaat cgtaaagaac agaagtcagt
     2821 tcaaactgct tatgatactg atgttcaaaa acgacaaata caaaatgcga cacagaatca
     2881 acaaagtaga caatctggaa atcgtaatca acctattaca cgtaattctc agtcaaaaga
     2941 ccgtctaaaa gagcaaaagg atatcaataa gcatggtaaa taaaaaagat attgctaaaa
     3001 aagtaatagg taaaacgcca ataggtgtta aattgaaatt agcgaaagtc atcatcattt
     3061 tgtgtgtggt ggcttttttt acatttccag taattgtaat gtttttgtta gcgcctgatt
     3121 taaataaagg taaagatgat gctggttgta ctgttagtgg cggtaatgtt tcggcaaatg
     3181 gtattgataa gtttaacgaa aatgctaaag gtggaaaatt agagggtaaa ggaaaagaaa
     3241 tacagaaaat tgcagaaaaa aataaagtac ctgtgaatat ttttatggcc attattgcga
     3301 gtgaatcaca atggggtaaa ggagaaaatg cgacaagaca aaataatcct ttgtctgtta
     3361 tgggttcaaa atcgattcat gattcaacat atccaactat tgaagatggt cttaatgcgg
     3421 gagctaaaaa tttgtatgat gtttatattt ctaaaggttt agatactcct aaaaaaatag
     3481 gtcccaaata tgcgcctgtt ggtgcgtcaa atgaccctaa taatatgaat gcccgttgga
     3541 taccaacagt tgaaaaaatt atgaaagatt taggcggttc agaagctaaa acgtcatgta
     3601 gtaatggtaa aggaaagtct ataaagttta atggtaaatt gccacattgg tcaaatgatg
     3661 accctggaaa aggtaattta tacactgctg gtcaatgtac ttggtatgca tacggtatgc
     3721 gtcaaaaaat gggtaaacct gtatcgactt attggcatga tgcacataaa tggaatgata
     3781 gagctaaagc tgagggttat aaagttgata aaaatccgga acctggtgca ttatttattg
     3841 ctgaacaagg tgcaggcggt catgatgggc attatggtca tgtggctgtt gttattggtg
     3901 taagtgatgg tggtaaaaca tttagaataa cagaaatgaa ttgggaaggt gcttttaaag
     3961 ttaatgaacg tacattgaaa atgactgacg gatatagttt tattcatgat aaggagtgaa
     4021 aataaatgaa gcaaaaaatt attatgttta ctcttgtaac agtaatatta ttttgtgctg
     4081 ttttaattgg ttatcagatt cctaaacaac aagttaaaat gaaacaaaat c
//
LOCUS       DAHHSC010000203         4103 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.204, whole genome shotgun sequence.
ACCESSION   DAHHSC010000203 DAHHSC010000000
VERSION     DAHHSC010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 4103)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4103)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4103
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.204"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            178..1521
                     /gene="brnQ"
                     /locus_tag="NBI57_002299"
     CDS             178..1521
                     /gene="brnQ"
                     /locus_tag="NBI57_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HCD1823227.1"
                     /translation="MNKNTWVIGFTLFAMFFGAGNLIFPPNLGLDSGQFFWPAILAFV
                     LTGIGLPLLGVIVGALDKEGYIGALNKISPKFSILFLIIIYLTIGPLFAIPRTASTSF
                     EMTITPIIHSNSSIALFIFTIIYFIVVLYICLNPSKLIDRIGSLLTPLLLITILAMII
                     KGYLDFSGNSAGKGNEALYHSNFSSFAEGFTQGYLTMDAIAAIAFSMIVVNAVKLTGI
                     TKTNQIFKQTLTAGLIAAVALIFIYISLGYIGNHMPVSDMTLDQLKSKDRNIGTYLLT
                     TMASTGFGSFGKYLLGIIVALACLTTACGLIVAVSEYFHRIVPKVSYKAFVLVFILMS
                     FIIANQGLNAVISMSIPVLSIVYPVAITVVLLILIAKFIPTKRISQQIPVIIVFILSI
                     FSVISKLGWLKINFIESLPLRAYSLEWFPVAIIATILGYLVGIFVKQDPIKYQQE"
     gene            complement(1594..2730)
                     /locus_tag="NBI57_002300"
     CDS             complement(1594..2730)
                     /locus_tag="NBI57_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HCD1823228.1"
                     /translation="MTENKSFKESHPLDDFISDKELSNTTIQKEKLTIEQQKQVDTIS
                     KQINPLDNEGLLAFGSDLQKQMSQFSHQMLDEVQSKDVGPIGDTLSDLMSKLKSVNPN
                     ELNTDKPSMLKRIFSRAKSSINEIFSRMQSVSAQVDRITIQLQKHQTHLTRDIELLDT
                     LYDKNKQYFDDLSLHIIAAQQKKLQLENEKLPQLQQQAQQSTNQMDIQQVADMQQFID
                     RLDKRIYDLQLSRQIALQTAPQIRMIQNVNQALAEKIQSSILTSIPLWKNQMAIALTL
                     MRQRNAVAAQRAVTDTTNDLLTANAEMLKQNAIETATENERGIVDLDTLKRTQRNIIE
                     TIEETLIIQQHGREERQLAEKELQQLEQDLKSHLVNIKGPNKQS"
     gene            complement(2762..3391)
                     /locus_tag="NBI57_002301"
     CDS             complement(2762..3391)
                     /locus_tag="NBI57_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     family protein"
                     /protein_id="HCD1823229.1"
                     /translation="MTVRYNISHIFGVLVGIPVAFLTSIFGMIALDVSFLIDMSIGIV
                     GFLMTYLPIQKLTSRKYLNEIGLTRKDYRYIRNQLNHTHQKLRGILKTYVNIRSIKDF
                     RQINDIYQISRSIYTTVRQRPASFYKVEGFFYSHIDNALNLVDAYTRLAKMPKKSINE
                     QQKLEQTRITLDEVKRTLIADLKRLNEDDYERLDIEMELNKLHQKHHQD"
     gene            complement(3410..3679)
                     /locus_tag="NBI57_002302"
     CDS             complement(3410..3679)
                     /locus_tag="NBI57_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HCD1823230.1"
                     /translation="MRHIHLQVFGRVQGVGFRYFTQRIAMNYNIVGTVQNVDDYVEIY
                     AQGDDADIERFIQGVIEGASPASNVTSHQLEELELNQKLSDFRSI"
     gene            3842..>4103
                     /locus_tag="NBI57_002303"
     CDS             3842..>4103
                     /locus_tag="NBI57_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HCD1823231.1"
                     /translation="MWTVTKIRADYEGWWLFSDWPENIVEKYQYQDFDDMFKHYQQLI
                     NQCKVQFDNYVTGKYNIYAFYNNCDMNYCEDCEEDLQIFYSFI"
BASE COUNT         1243 a          635 c          651 g         1574 t
ORIGIN      
        1 agttactatt taaaaagtga atttgaagaa ttttattttc tggcataatt tgatttaaac
       61 aacacatgtg ttttgtttct gctatgtaca acatatttca tatagactct ttattatttt
      121 ttgtgctaaa atatttactt gtaaattatt tcgtgtttta aaaaaaggag taactctatg
      181 aataaaaata catgggtcat tgggtttacg ctcttcgcta tgttttttgg cgcaggcaat
      241 ctaatttttc ctcctaatct cggattggat agtggtcaat ttttctggcc cgccatttta
      301 gcgtttgttc taactgggat tggtttacca ttattaggtg tgattgtagg tgcacttgat
      361 aaagaaggat atattggcgc attaaataaa atttcaccta aattttcaat attgttctta
      421 atcatcattt atttgactat aggaccactt ttcgcaatac ctagaactgc atctacatct
      481 tttgaaatga caattacacc aattatacat agcaatagta gtatcgcttt atttatattt
      541 acgattatct acttcatagt cgttttgtat atttgtttaa atccatctaa gttaatcgat
      601 cgtattggtt cattattaac accattatta ttgattacta ttttagcgat gattattaaa
      661 ggatacttag actttagcgg taatagtgct ggaaagggca atgaagcact atatcattct
      721 aatttttcaa gttttgctga aggctttaca caaggctatt taacaatgga tgccattgca
      781 gcaattgctt tttcaatgat tgttgttaat gcagtaaaac taacaggcat tactaaaaca
      841 aatcaaatat tcaaacaaac tttgactgct ggtttaattg cagccgtagc tttaattttc
      901 atatatattt cattaggtta tattggtaat catatgccag taagtgacat gacgttagat
      961 caattgaaat ccaaagatcg aaacattggg acatatttat taacgacaat ggcttcaaca
     1021 ggatttggtt cattcggaaa atatttattg ggcatcattg tggcgctggc atgtctaact
     1081 acagcatgcg ggcttattgt tgcagtttct gaatatttcc atagaatcgt acctaaagta
     1141 tcatacaaag catttgtatt agttttcatt ttaatgagtt ttattattgc taaccaaggt
     1201 ttaaatgctg ttatctcaat gtcaattccg gtattaagca ttgtataccc agtagcaata
     1261 actgttgtat tattaatttt aattgccaaa ttcataccga caaaacgcat ttcacaacaa
     1321 attccagtta ttatcgtatt tatattgtcg attttcagtg ttattagtaa gttaggttgg
     1381 ctgaaaatta actttataga atcattgcct ctaagagcgt attctttaga gtggttccca
     1441 gtagcaatta ttgcaacgat attaggctat ctagtcggca tatttgtaaa acaagatcca
     1501 attaaatatc aacaggaata acgaataata taaaaggaag catctccaat ttaatcgtag
     1561 gatgcttcct ttttgttata tctaattcgt aatttatgat tgtttattcg gtcctttgat
     1621 gttcactaaa tgtgacttta aatcttgttc taattgttgt aattcttttt cagctaattg
     1681 tcgttcttcg cgaccgtgtt gttgaataat taatgtttct tcaattgtct caataatgtt
     1741 acgctgtgta cgtttcaatg tatcaagatc aacaatgcca cgctcatttt ctgttgcagt
     1801 ttcaatcgca ttttgtttca acatttcagc atttgctgtt aataaatcat tagttgtatc
     1861 agtgacagct cgttgtgcag caactgcatt acgctgtctc attaatgtaa gcgcaatggc
     1921 catttgattt ttccatagtg gaatacttgt caaaattgaa ctttgtatct tctcggcaag
     1981 tgcttgatta acattttgaa tcatacgaat ttgtggcgca gtttgcaaag ctatttgtct
     2041 tgaaagctgt aagtcatata tgcgtttatc tagtctatct ataaattgct gcatatctgc
     2101 aacttgttga atatccattt gattagtgga ttgctgcgct tgctgttgca attgtggtag
     2161 cttttcattt tctaattgca actttttttg ctgtgcagca atgatatgca atgataagtc
     2221 atcaaagtat tgtttgtttt tatcatatag cgtatctaat aattcaatat ctcttgttaa
     2281 atgtgtttga tgtttctgca gttgaatcgt tatgcgatcg acttgagcac taactgattg
     2341 cattcttgaa aagatttcat tgatagacga ctttgctctg ctaaaaattc tttttaacat
     2401 agatggttta tcagtattta actcatttgg attaactgac tttagttttg acattagatc
     2461 tgacaaagta tctccaatag gaccaacatc tttactttgt acttcatcca acatttgatg
     2521 tgaaaattga gacatttgtt tctgtaaatc agaaccaaac gctaataaac cttcattgtc
     2581 taaagggtta atttgtttac tgattgtgtc tacctgtttt tgttgttcaa ttgttaactt
     2641 ttctttttga atagtagtat tcgataattc tttatcgctt ataaaatcat ctagtggatg
     2701 gctttctttg aaacttttat tttcagtcat caatcatctc tccaatagtt gctattttac
     2761 atcaatcttg atgatgtttt tgatgtaact tatttaattc catttcaata tctaaacgtt
     2821 cataatcatc ttcgttgaga cgctttaaat cagcgattaa tgttcgtttg acctcatcca
     2881 aagtaattcg tgtttgttct aacttttgct gttcattaat tgattttttg ggcatttttg
     2941 ctagacgtgt atatgcatca accaaattta aagcattatc aatatgagaa taaaaaaagc
     3001 cttcaacttt ataaaatgat gcaggtctct gtctaactgt cgtataaata gaacgtgaaa
     3061 tttggtatat atcattaatc tgcctaaaat ctttaattga tcttatattg acatacgttt
     3121 ttaaaatacc tctaagtttt tggtgtgtat gatttaactg atttcgaata tagcgatagt
     3181 cttttctagt caaaccaatt tcgtttaaat atttgcgtga agtgagtttt tgtatcggta
     3241 ggtatgtcat taaaaagcca acaataccaa tagacatatc aattaaaaaa gatacatcaa
     3301 gtgcaatcat cccaaatatg cttgttaaaa acgctacagg aattcccact aacaccccaa
     3361 atatatgaga aatattatat ctcactgtca tcttccttta tttagcattt tatattgatc
     3421 gaaaatccga taatttttga tttagttcta actcttcaag ttgatggctt gttacatttg
     3481 atgctggtga ggcaccttca attacacctt gaataaatct ctctatatct gcgtcatccc
     3541 cttgtgcata tatctctaca tagtcatcta cattttgaac agtaccgaca atgttatagt
     3601 tcattgcaat gcgttgtgta aaatatctaa atccgacgcc ttgaacgcgt ccgaatactt
     3661 gtaaatgtat atgtctcatt tttaccacct cataatgtta ttatacgtag ttttacttaa
     3721 aaaaactaat aattactata gttactactt tgtttgtttc aagtcgtcaa acttgatttt
     3781 cagaggataa aggtataaaa ataagtatag agtttttgaa gtatggaagg ggtctttaat
     3841 aatgtggaca gttaccaaaa ttagagccga ttatgaggga tggtggttat tcagtgactg
     3901 gccagaaaac attgttgaaa aatatcaata tcaagatttt gatgacatgt ttaagcacta
     3961 tcaacaattg attaatcaat gtaaagttca gttcgataat tatgtcacag gcaaatataa
     4021 tatttatgca ttttataata attgtgacat gaactattgc gaagattgtg aagaggattt
     4081 acaaatattt tatagtttca ttg
//
LOCUS       DAHHSC010000204         4075 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.205, whole genome shotgun sequence.
ACCESSION   DAHHSC010000204 DAHHSC010000000
VERSION     DAHHSC010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 4075)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4075)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4075
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.205"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            <1..1377
                     /locus_tag="NBI57_002304"
     CDS             <1..1377
                     /locus_tag="NBI57_002304"
                     /EC_number="2.7.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulokinase"
                     /protein_id="HCD1823232.1"
                     /translation="DFTSSTIIFTDENLNPVHNLKQFKNNPHAYVKLWKHHGAYKEAE
                     KLYQTAIENNNKWLGHYGYNVSSEWMIPKIMEVMNRAPEIMEKTAYIMEAGDWIVNKL
                     TNKNVRSNCGLGFKAFWEEETGFHYDLFDKIDPKLSKVIQDKVSAPVVNIGEVVGKLD
                     DKMAQKLGLSKETMVSPFIIDAHASLLGIGSEKDKEMTMVMGTSTCHLMLNEKQHQVP
                     GISGSVKGAIIPELFAYEAGQSAVGDLFEYVAKQAPKSYVDEAANRNMTVFELMNEKI
                     KHQMPGESGLIALDWHNGNRSVLSDSNLTGCIFGLTLQTKHEDIYRAYLEATAFGTKM
                     IMQQYQDWHMEVEKVFACGGIPKKNAVMMDIYANVLNKKLIVMDSEYAPAIGAAILGA
                     VSGGAHNSINDAVDAMKEPILYEINPEAEKVQRYETLFKAYKALHDIHGYKKANIMKD
                     IQSLRVEG"
     gene            1591..2556
                     /locus_tag="NBI57_002305"
     CDS             1591..2556
                     /locus_tag="NBI57_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HCD1823233.1"
                     /translation="MKKIMITGALGQIGTELVVKCREIYGTDNVLATDIREPEADSPV
                     QNGPFEILDVTDRDRMFELVRDFEADSLMHMAALLSATAEKNPILAWDLNMGGLMNAL
                     EAARTYNLHFFTPSSIGAFGDSTPKVNTPQVTIQQPTTMYGVNKVAGELLCQYYFKRF
                     GVDTRSVRFPGLISHVKEPGGGTTDYAVEIYFKAVREGHYTSFIDKGTYMDMMYMDDA
                     IEAIIKLMEADDAKLETRNGYNLSAMSFDPEMVKEAIQEYYPNFTLDYDVDPIRQGIA
                     NSWPDSIDTSCSRGEWGFDPKYDLASMTKLMLEAIEQKDTVKNNN"
     gene            2892..3968
                     /locus_tag="NBI57_002306"
     CDS             2892..3968
                     /locus_tag="NBI57_002306"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HCD1823234.1"
                     /translation="MSQAVKVERRETLKQKPNTSQLGFGKYFTDYMLSYDYDADKGWH
                     DLKIVPYGPIEISPAAQGVHYGQSVFEGLKAYKRDGEVALFRPEENFKRLNNSLARLE
                     MPQVDEAELLEGLKQLVDIERDWIPEGEGQSLYIRPFVFATEGALGVGASHQYKLLII
                     LSPSGAYYGGETLKPTKIYVEDEYVRAVRGGVGFAKVAGNYAASLLAQTNANKLGYDQ
                     VLWLDGVEQKYIEEVGSMNIFFVENGKVITPELNGSILPGITRKSIIELAKNLGYEVE
                     ERRVSIDELFESYDKGELTEVFGSGTAAVISPVGTLRYEDREIVINNNETGEITQKLY
                     DVYTGIQNGTLEDKNGWRVVVPKY"
BASE COUNT         1495 a          524 c          810 g         1246 t
ORIGIN      
        1 gactttactt catctactat tatttttact gacgaaaacc ttaacccggt acataattta
       61 aaacaattta aaaacaatcc acatgcgtat gtgaaacttt ggaaacatca tggtgcatat
      121 aaagaagcag agaaattata tcaaactgct attgaaaata ataataagtg gttaggccat
      181 tatggatata atgttagtag tgaatggatg attcccaaaa taatggaagt catgaatcga
      241 gcaccagaaa ttatggaaaa aacggcttat attatggaag cgggcgattg gattgtaaat
      301 aaattaacta ataaaaatgt acgctcgaat tgtggattag gtttcaaagc attttgggaa
      361 gaagaaacag ggtttcatta tgatttattt gataaaatag accccaaatt atcaaaagta
      421 attcaagata aagtatctgc accggttgtt aatattggtg aagtagtagg gaaactggac
      481 gataaaatgg cacagaaatt aggattatca aaagagacta tggtaagtcc ttttattatt
      541 gatgcccatg ctagtttatt aggtattggg tctgaaaaag ataaagaaat gactatggtg
      601 atgggaacaa gcacatgcca tcttatgtta aatgaaaagc aacatcaagt gccaggtata
      661 tcaggttctg taaaaggagc aattattcca gaattatttg cttatgaagc ggggcaatca
      721 gcagtaggtg atttgtttga gtatgtcgct aagcaagcac caaagtcata tgtagatgaa
      781 gcagcaaata gaaatatgac tgtatttgaa ttaatgaatg aaaagataaa acatcaaatg
      841 ccaggtgaaa gtgggctcat tgctcttgat tggcataatg gaaatcgaag tgtattaagt
      901 gatagcaatt taacaggttg tatctttgga ttaactttac aaactaagca tgaggatatt
      961 tatagagcat atttagaagc tacagcattt ggtactaaga tgattatgca acagtatcaa
     1021 gattggcata tggaagtaga aaaggtattt gcatgtggcg gtatacctaa aaagaatgct
     1081 gttatgatgg atatctatgc gaatgtactg aataaaaaac taattgttat ggatagtgag
     1141 tatgcaccag caataggcgc agcaatatta ggtgcagtca gtggtggcgc acataattca
     1201 attaatgatg cagttgatgc tatgaaagag ccaattttat acgaaattaa tccagaagcg
     1261 gaaaaagtac aaaggtatga aacattattt aaagcttata aggctttaca tgatatccat
     1321 ggttataaaa aagctaatat aatgaaagat atccagagtt taagagttga gggataaaaa
     1381 atttaatttg acagtaatta gcaataataa acgctataaa ttactaaaat caattaatac
     1441 aactagaatt tccatttaca gaaacatcag caacatgtac atgtcatata tacaagaaag
     1501 cgcttttata ttataattat tatgaaaatg aatattatgt ccgcttgtga cggactatag
     1561 ctatatagaa gagacaaagg agatatttct atgaaaaaaa ttatgattac tggtgcatta
     1621 ggacaaattg gtacagaatt agttgttaag tgcagagaaa tttatgggac agataatgtt
     1681 cttgctacag atattaggga acctgaagca gactcacctg tacaaaatgg accatttgaa
     1741 attttagacg taacagatcg tgaccgcatg tttgagttag ttagggactt tgaagcggat
     1801 agtctaatgc atatggcagc attattatca gcaactgcag agaaaaatcc aattctagct
     1861 tgggatttaa atatgggtgg attaatgaat gcattagaag ctgcaagaac ttataatttg
     1921 cactttttca caccaagttc aattggtgca tttggagact caactcctaa agttaatacg
     1981 ccacaagtaa cgattcagca acctacgaca atgtatggtg taaataaagt agctggagaa
     2041 ttattgtgtc aatactattt caaacgtttt ggtgtagata caagaagtgt tagattccca
     2101 ggtttaatct cgcatgttaa agagccaggt ggcggtacta cagactatgc tgttgaaata
     2161 tacttcaaag cagtaagaga gggtcattat acaagcttca tagataaagg cacgtatatg
     2221 gatatgatgt atatggatga tgcaattgaa gcaattatta aacttatgga agcagacgac
     2281 gctaaattag aaactagaaa cggttataat ttgagcgcaa tgagttttga tccagagatg
     2341 gtaaaagaag caattcaaga atactatcca aattttacat tagattatga tgttgatcct
     2401 attagacaag gtatcgctaa tagttggccg gattctattg atacaagctg ttcacgtggc
     2461 gaatggggat ttgatcctaa atatgattta gcgagcatga ctaaattaat gttagaagct
     2521 attgaacaaa aagatactgt taaaaataat aactaatcat ttccattcac tttaatacac
     2581 ggaatgatat tttaaattac tctttatttt aataaactag tgcatgaatt ctaatattat
     2641 tcattataca tattgaattc gcgagcttag ttcattttaa agtaaagagt tttttgtatg
     2701 taaagtatat tagtaaaaca cagattactt gtctctgtga aattaatacg tttatactaa
     2761 aagatatagt ctttctagaa aatctgttaa ttaattttga atttttagaa aatttattga
     2821 acagcaaaat atggattgtt ataatttaag ttaaacaaat tcttatacaa tattattagg
     2881 aggcaatcac catgtcacaa gcagttaaag ttgaacgacg agaaacatta aaacaaaaac
     2941 caaatacatc tcaactaggt tttggtaaat attttactga ttatatgttg agttatgatt
     3001 atgatgcaga taaaggatgg catgatttga agatagtacc ttatggtcct attgaaattt
     3061 cacctgctgc acaaggtgtt cattatggtc aatcggtatt cgaaggatta aaagcatata
     3121 aaagagatgg ggaagttgca cttttccgtc ctgaagaaaa ttttaagcgt cttaataact
     3181 cgttagcacg attagaaatg cctcaagtag acgaagcaga attgttagag gggctaaaac
     3241 aattagttga tatagaaaga gattggattc ctgaagggga aggtcaatca ttatatattc
     3301 gtccatttgt ttttgcaaca gaaggggcac ttggcgttgg tgcatcacat cagtataaat
     3361 tattaattat tttatctcct tcaggtgcat attatggtgg tgagacttta aaaccaacta
     3421 aaatctatgt agaagatgaa tatgtgcgtg ctgttcgtgg cggtgtaggc tttgcaaaag
     3481 ttgcaggtaa ctatgcggca agtttattag cacaaacaaa tgcaaataaa ttaggttatg
     3541 accaagtatt atggcttgat ggtgttgaac agaaatatat cgaagaagtt ggtagcatga
     3601 atattttctt cgttgaaaat ggaaaagtaa ttacaccaga gttgaatggc agtattttac
     3661 ctggtattac acgtaaatct attatcgaat tagctaaaaa cttaggatat gaagtcgaag
     3721 agcgccgcgt ttcaatcgat gaattattcg aatcatatga taaaggtgag ttaacagaag
     3781 tatttggtag tggtactgca gcagttattt cacctgtggg tacattgaga tacgaagatc
     3841 gtgaaatcgt tattaataat aatgagactg gtgaaattac tcaaaaatta tacgacgtct
     3901 atactggtat tcaaaatggt actttagaag ataaaaatgg ttggagagtc gttgtaccaa
     3961 aatattaata aaaattgaat ataatcatga aaacaatatg taaaagttaa tcatgactaa
     4021 aatccctttc aatgaagcga agcatggtaa taaattaagt ttacaatgtt tattg
//
LOCUS       DAHHSC010000205         4048 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.206, whole genome shotgun sequence.
ACCESSION   DAHHSC010000205 DAHHSC010000000
VERSION     DAHHSC010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 4048)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4048)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4048
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.206"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            243..1190
                     /locus_tag="NBI57_002307"
     CDS             243..1190
                     /locus_tag="NBI57_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HCD1823235.1"
                     /translation="MPGIIQIDDMNQSQALIGNNDEHLKAIEESFDVVIHARGQEVAV
                     KGTKIENVEKAESVLINLLKVIDLGNNITIKDVEAAIKMAHNNTIQHLLDLYDEEITK
                     DAFGKTIRAKTMGQRIYVNAMKNNDLVFGIGPAGTGKTFLAVVYAAKQLRKGAVKRIV
                     LTRPAVEAGESLGFLPGDLKEKVDPYLRPLYDGLYTVLGREQTERFIERGIIEIAPLA
                     YMRGRTLEDAFVILDEAQNTTHAQMKMFLTRLGFGSKMVVTGDQTQIDLPKGVKSGLK
                     EAVSRLHNVKGISILKLDQSDVVRHPLVSKIIEHYEGEN"
     gene            1191..1658
                     /gene="ybeY"
                     /locus_tag="NBI57_002308"
     CDS             1191..1658
                     /gene="ybeY"
                     /locus_tag="NBI57_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HCD1823236.1"
                     /translation="MFTIDFSDHTGLVKDAWYKQIEDLLEFAKKEEHIEDDAELSVTF
                     VDKQEIQEINRTYRDKDKVTDVISFALEEDEPEIDFSGLDIPRVLGDIIICTDVAQEQ
                     ANNYGHSFERELGFLALHGFLHLLGYDHMTEADEKEMFGRQDTILNAYGLTRD"
     gene            1661..2005
                     /locus_tag="NBI57_002309"
     CDS             1661..2005
                     /locus_tag="NBI57_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HCD1823237.1"
                     /translation="MKRFKYALDGLKILIQKDYKFLLHVFAMIVAIVFGLVLNINRIE
                     WIFILIAIALVLTVEALNTAIEYVVDLVTVEYHDLAKYAKDIAAFSVLIVSILAFIIG
                     LIVFLPHFIALF"
     gene            2016..2420
                     /gene="cdd"
                     /locus_tag="NBI57_002310"
     CDS             2016..2420
                     /gene="cdd"
                     /locus_tag="NBI57_002310"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HCD1823238.1"
                     /translation="MSYQPHYFQEVRKAQQESYSPYSQFKVGAYLKTKDGRTFYGTNV
                     ENASYPLSICAERASLVSAISQGYRPGDFESITVTVDADKPSSPCGACRQVLKELCDD
                     DMPVYMTNHKGDMVMMTVAELLPFGFSGKDLE"
     gene            2421..3320
                     /gene="era"
                     /locus_tag="NBI57_002311"
     CDS             2421..3320
                     /gene="era"
                     /locus_tag="NBI57_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000134760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HCD1823239.1"
                     /translation="MTEHKSGFVSIIGRPNVGKSTFVNRVIGHKIAIMSDKAQTTRNK
                     IQGVMTRDDAQIIFIDTPGIHKPKHKLGDYMMKVAKNTLSEIDAIMFMVNANEEIGRG
                     DEYIIEMLKNVKTPVFLVLNKIDLVHPDELMPKIEEYQSYMDFTEIVPISALEGLNVD
                     HFIDVLKTYLPEGPKYYPDDQISDHPEQFVVGEIIREKILHLTSEEIPHAIGVNVDRM
                     VKESEDRVHIEATIYVERDSQKGIVIGKGGKKLKEVGKRARRDIEMLLGSKVYLELWV
                     KVQRDWRNKVNFIRQIGYVEDQD"
     gene            3348..>4048
                     /gene="recO"
                     /locus_tag="NBI57_002312"
     CDS             3348..>4048
                     /gene="recO"
                     /locus_tag="NBI57_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HCD1823240.1"
                     /translation="MRQKGIIIKAVDYGESDKIITILNEHGAKVPLMARRAKKVKTGL
                     QAQTQLFVYGLFIYNQWRGMGTLNSVDVISQHYKLQMDLYVSSYASLAAETIERSMDE
                     GDIAPYNYQLLQFVLEKIESGTSAQLMSVVVMLKCMKRFGFTASFNRCAVSGNDTQAD
                     LIGYSFKFDGAISRQEASKDVHAVILSNKTLYLLDVLQKLPIDKMNSLNIHQEIIDEM
                     SDIILMLYREYAGMF"
BASE COUNT         1476 a          514 c          811 g         1247 t
ORIGIN      
        1 ttaaaaaaca aagagaatct ttcgagaaat attagttatt taaattacag caaaaaattg
       61 attagtctaa aattgaaact gcttttatga caaggtgaaa agtataaatg attattttaa
      121 attaaagaaa aatgagtaag tcaatgcaaa gatgtttaaa tcaatcaatt gcatgatata
      181 attaagtaga tattaaagca tcatagaatg aatataaatg atatatgaaa aggagcgcgt
      241 gtatgcctgg aattatacaa atagacgata tgaaccaatc tcaagcttta attggaaata
      301 atgatgaaca tttaaaagca attgaagaga gtttcgatgt tgtcatccat gcaagaggac
      361 aagaagttgc cgttaaaggt acaaaaatag aaaacgtaga aaaagcggaa tcagtattaa
      421 tcaatttgct gaaggttatt gatttaggta ataatattac aattaaagat gttgaagcag
      481 ctattaaaat ggcgcataat aacacaattc aacatctgtt agatttatat gatgaagaga
      541 taactaaaga tgcatttggt aagacgattc gtgcgaaaac gatggggcaa cgtatatatg
      601 ttaatgccat gaaaaataat gatttagtat ttggtatagg tcctgctggt acaggtaaga
      661 cattcttagc tgtagtttat gcagcaaagc aactccgtaa aggtgctgtt aaacgtattg
      721 tattaacaag acctgctgtt gaagcaggag agtcacttgg atttttacca ggagatttga
      781 aagaaaaggt agatccatat ttaagacctt tatatgatgg tctatatact gttcttgggc
      841 gtgaacaaac agagcgattt attgaaagag gcattatcga aatagcgcca cttgcatata
      901 tgcgcggacg aacattagaa gatgcatttg taattcttga tgaggcgcag aatacgacac
      961 atgcgcaaat gaaaatgttt ttaacaagac taggttttgg ctcaaaaatg gtagttactg
     1021 gtgaccaaac tcaaatcgat ttacctaaag gtgttaaaag tggacttaag gaagcggtca
     1081 gtaggttaca caacgttaaa ggtataagta tattgaaatt agatcagagc gatgtagtaa
     1141 gacatccatt ggtaagtaag atcattgaac attatgaagg agagaattaa atgtttacga
     1201 tagattttag cgatcacaca ggcttagtta aagatgcgtg gtataaacaa attgaagatt
     1261 tattagaatt tgctaaaaaa gaagagcata tagaagacga tgctgagctt tctgttacat
     1321 ttgtagataa acaagaaata caagaaatta atcgaacata tagagataaa gataaagtta
     1381 cagatgtaat ctcatttgct ttagaagaag atgagccaga gattgatttt agtggtcttg
     1441 atataccacg tgttttaggg gatataatta tctgtacgga tgtagcgcaa gaacaagcaa
     1501 acaattacgg acattctttt gaacgagaat taggattttt agcattacat ggatttttgc
     1561 atctattagg ttatgatcat atgactgaag cggatgaaaa ggaaatgttt ggtcgacaag
     1621 atacaatatt aaacgcttat ggattaacac gagactaatt atgaaaaggt ttaaatatgc
     1681 acttgatggg ctgaaaatct taattcaaaa agactataaa tttcttttac atgtgtttgc
     1741 aatgattgtt gctattgtct ttggtctcgt actaaatatt aatcgtattg agtggatatt
     1801 tatactcatt gctattgcat tagttctcac tgttgaagct ttaaacactg ctattgaata
     1861 tgttgtcgat ttagtgaccg ttgaatatca tgatttagct aaatacgcaa aagatatagc
     1921 ggcttttagt gtacttatag tttcaatatt agcatttatt ataggtttaa tagtattttt
     1981 accacatttt atagcgttat tttagggagg catatatgag ttatcaacct cattattttc
     2041 aagaagttag aaaagcacaa caagaatcat attcgccata cagtcaattt aaagtagggg
     2101 cttatttaaa aacgaaagac ggtagaactt tttatggtac caatgtagaa aatgcttctt
     2161 atccattatc gatatgtgct gaacgagcta gtttggtatc ggcaatttct caaggataca
     2221 gaccaggtga ttttgaatca ataactgtaa ccgtagatgc agataaaccg tcatcacctt
     2281 gtggtgcatg tcgtcaagtt ttaaaggaat tatgtgatga tgatatgcct gtgtatatga
     2341 caaatcataa aggagatatg gttatgatga cagtcgcaga gttactacca tttggatttt
     2401 caggaaagga tttagaataa atgacagaac ataaatcagg atttgtttca attataggta
     2461 gaccaaatgt agggaagtca acatttgtta atagagtgat tggccataaa atagcaatca
     2521 tgtccgataa agctcaaaca actagaaata aaattcaagg tgttatgaca agagatgacg
     2581 cgcaaattat attcattgat acgccaggta ttcataaacc taaacacaaa ttaggtgact
     2641 atatgatgaa agtcgctaaa aatacattat ctgaaataga tgcaatcatg tttatggtta
     2701 atgccaatga ggaaattgga cgaggcgatg aatatattat agaaatgttg aaaaatgtta
     2761 agacaccagt atttttagta ttaaataaaa tagatttagt gcatccagat gaattaatgc
     2821 caaagattga agaatatcaa agttatatgg actttacaga gattgtacct atttcagcat
     2881 tagaagggct aaatgtagat cattttattg atgttttaaa gacgtattta cccgaaggac
     2941 ctaaatatta tccagatgat caaatttcag accatcctga acaatttgta gtgggtgaaa
     3001 tcattcgtga aaaaatcctt catcttacaa gtgaagaaat ccctcatgcg attggtgtta
     3061 atgtggaccg tatggttaaa gaaagcgaag atcgtgttca tatcgaagca actatatatg
     3121 ttgaaagaga ttcgcaaaaa ggaattgtca ttggaaaagg cggtaaaaag ttaaaagaag
     3181 taggaaaacg tgcgagacgt gatatagaaa tgcttctagg ctctaaagta tacttagaat
     3241 tatgggtcaa agttcaaaga gactggcgaa acaaagttaa ctttattcgc caaattggtt
     3301 atgttgaaga ccaagattaa tcttaaaagt ggtgaagata attgttaatg cgccaaaaag
     3361 ggattatcat caaagcagtt gattatggtg aatctgataa aattatcacg attttaaatg
     3421 agcatggtgc aaaagtacca cttatggcaa ggcgtgctaa aaaagttaag acgggtttac
     3481 aagcgcaaac gcaattgttt gtttatggtt tgtttattta caatcagtgg cgaggtatgg
     3541 gaacgttaaa ttctgtagat gttattagtc aacattataa attacaaatg gacctttacg
     3601 taagcagtta tgcctctctg gcagctgaaa ctattgagcg ctcaatggat gaaggtgaca
     3661 ttgcaccata taactatcaa ttattacaat ttgttcttga aaaaatagaa tcaggtacat
     3721 ctgcacagtt aatgtcagtc gtagttatgt taaagtgcat gaaacgattt ggttttactg
     3781 catcatttaa tcgctgtgct gtgagtggta atgacacaca agcagattta ataggttata
     3841 gttttaagtt tgacggtgcg atttcaaggc aagaggcttc taaagatgta catgcagtta
     3901 tattatcgaa taaaacacta tatttattag atgtattaca aaaattaccg atagataaaa
     3961 tgaattcatt gaatatccat caagaaatta ttgatgaaat gtcagatatc attttaatgt
     4021 tatatcgtga atatgcaggt atgttttt
//
LOCUS       DAHHSC010000206         3996 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.207, whole genome shotgun sequence.
ACCESSION   DAHHSC010000206 DAHHSC010000000
VERSION     DAHHSC010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3996)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3996)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3996
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.207"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            114..851
                     /gene="sdpB"
                     /locus_tag="NBI57_002313"
     CDS             114..851
                     /gene="sdpB"
                     /locus_tag="NBI57_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease SdpB"
                     /protein_id="HCD1823241.1"
                     /translation="MENEKKKYTFKDIAWRDLLIIPIIGVIFYALTIAAYIGPILFGY
                     YEIDSNLIFVATITQALAYTIGIGAFYLFHLKVMPSRLRAGFAYIKKYWLRLSITYII
                     ALILIYTYEYMTQFLPKHLQYSETANELELNKMFEVPAFLPVAFLLIVIVGPIVEEIV
                     FRHILIGELGKKFNFIAMSIVSVFLFAFIHVTDAKSPFEFGPYLILSIILVFTYLKSG
                     RNLGSTIALHIANNFVSFVISVISIYA"
     gene            complement(876..1568)
                     /locus_tag="NBI57_002314"
     CDS             complement(876..1568)
                     /locus_tag="NBI57_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YafY family transcriptional regulator"
                     /protein_id="HCD1823242.1"
                     /translation="MNKAERQNLIITAIQQNKKMTALELAKYCNVSKRTILRDIDDLE
                     NQGVKIYAHYGKNGGYQIQQAQSKIALNLSETQLSALFLVLNESQSYSTLPYKSEINA
                     IIKQCLSLPQTRLRKLLKRMDFYIKFDDTQHMTLPMLFSDILIYCTERNVMLVDHRVD
                     DNIKAENVIFIGLLCKHGHWHAVIYDIAQDKTAELEIENIIDISYSFGKTIQTRDISI
                     DNYHQFLNPIDS"
     gene            1954..2751
                     /locus_tag="NBI57_002315"
     CDS             1954..2751
                     /locus_tag="NBI57_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase"
                     /protein_id="HCD1823243.1"
                     /translation="MTDKTLQQIDKLICSWLKQIDNVIPQLIMEMTTETKRHRFDLVT
                     NVDKQIQQQFQQFLATHFPEHQLLAEEKSNEMITNEINHLWIMDPIDGTANLVKQQED
                     YCIILAYFYEGKPMLSYVYDYPHKKLYKAIRGEGAFCNGIKMEEPPSLKLEDAIISFN
                     AQVMNLDTVQDLFDASFSYRLVGACGLDSMRVAKGQFGAHINTNPKPWDIAAQFLFAE
                     LLNLKMTTLDGKAIDHLKGAPFIISNKACHETVLKILNANGGYQKYR"
     gene            2884..3831
                     /locus_tag="NBI57_002316"
     CDS             2884..3831
                     /locus_tag="NBI57_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000830946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HCD1823244.1"
                     /translation="MKRSSKSKMSLPKKIFLWVFGILVILAIVAVVYVAAKIFITGNK
                     IHNPLDRNHSELRDKKVSLNDGDPFTIALFGVDSDADRKKKGGGERSDSIMILSINPK
                     TKKTEIVSIPRDTRAEIVGRGTTEKIAHAYAYGGPNMAVKSLEKLMNVPIDHYATIDM
                     DGLHNMIDSIGGVDVVSNDTFTVDGVRFTKGQQTHVNGDQALKFIRSRKEEGAGGDFG
                     RQQRQQIVLEAMANKIASPSSITHFNSLMNEIQNNVKTDLTLGDLNTIRSNYKDANDT
                     INKHQLSGQGGIQSDGLYYFIPSEQSKAESTKLLKDNLE"
BASE COUNT         1404 a          535 c          724 g         1333 t
ORIGIN      
        1 atcgtatcaa tgatttgaat caaattagta atttgttgtc tcatatactt agtttcgttt
       61 atcatgtaga agtacctcca aaaatcaatt attaaacagg aaaggatata acagtggaaa
      121 atgaaaaaaa gaaatacacg tttaaagata tagcatggag agatttgctt ataattccaa
      181 ttataggtgt tatattttat gcactcacaa ttgcagctta cataggacca attttgtttg
      241 gttattatga aatcgatagt aatctgatat ttgtagcgac tatcacacaa gctttagcat
      301 atacgattgg gattggcgca ttttatttat ttcatttaaa agtgatgcca tcaaggcttc
      361 gagctggttt tgcgtatatc aaaaaatatt ggcttagatt gtcaataacg tatattattg
      421 ctctcatctt aatttataca tatgaatata tgacacagtt tctacctaaa catttgcaat
      481 atagtgaaac agccaatgaa cttgagctaa ataaaatgtt tgaagtgcct gcatttttac
      541 ctgtagcatt cttattaatt gtgattgtag gtccaatagt tgaagaaatc gtatttagac
      601 atattttaat tggagaatta ggtaagaagt ttaattttat agctatgagt attgtttcgg
      661 tgttcttatt tgcatttatt catgtgactg atgcaaagtc gccgtttgag tttggtccat
      721 atctcatact atccatcata ttagttttca cttatttaaa atctggaaga aacctaggtt
      781 caacaattgc attacatata gcaaataatt ttgtttcttt cgttatatca gtaataagta
      841 tttatgcgta gataaaaccc catcgactcc atagtttagg aatcgatggg gtttaaaaat
      901 tgatgatagt tatcaatgga tatgtctctg gtttgaatcg tcttaccgaa tgaatacgaa
      961 atatctataa tattttcaat ttcgagttcg gcagttttgt cttgagcaat gtcataaatg
     1021 actgcatgcc aatgtccatg tttacacaaa aggccaataa atataacgtt ttcagcttta
     1081 atattatcat caaccctatg atctactaac atcacatttc gttctgtaca ataaattaaa
     1141 atgtcggaaa acagcattgg gagtgtcata tgttgtgtgt catcaaattt aatataaaag
     1201 tccatgcgtt taagcaattt tcttaagcgt gtttgtggaa gacttaaaca ttgttttata
     1261 attgcgttga tttcgctttt atatggtaat gtcgagtacg actgactttc attaagcact
     1321 aaaaataagg ccgataattg tgtttcagat aagtttaatg caattttaga ttgtgcttgt
     1381 tgtatttggt aaccaccatt tttcccataa tgcgcataaa ttttaacacc ttgattttct
     1441 aaatcatcaa tatctcttaa aattgtgcgt ttggatacgt tgcaatattt agctaattct
     1501 aaagcggtca tttttttatt ttgttgaatt gcagtaatta ttaaattttg cctttcagct
     1561 ttattcatga cagccacctc ctaaaagatg atatattaca caaagtataa cataagagtg
     1621 acaatgagga cattttcaat atcttttaaa aaaaatcgta aaaatttttt tggaaaacgc
     1681 ttacatttta aaaaagattg tgctatgata ttacttgtaa cagggggagc gattaaacaa
     1741 aggggtagag ctatataaca gatagctttt aatcgttcaa gttacatcac aacatcttca
     1801 atatttatta cttactttcc tttctatttg tcggctagca cgtgactagc cgattttttt
     1861 atgcataaaa tgaagaagtc ttcagttgta ttgttgattc ttttaatata gggtaataat
     1921 aaaatgataa tgatatgaga agtggtggac gaaatgacag ataaaacatt acagcaaata
     1981 gataagttaa tatgttcgtg gttgaaacaa atagacaatg ttattccgca attaattatg
     2041 gaaatgacta cagaaactaa gcgtcatcga tttgatttag ttacaaatgt agataaacag
     2101 attcaacaac aattccaaca atttttagca acacattttc cggaacacca actattagcg
     2161 gaagaaaaaa gtaatgaaat gattacgaat gaaattaatc atttatggat tatggatccc
     2221 attgatggaa ctgccaattt agtaaaacag caagaagatt attgtattat attggcgtat
     2281 ttttatgaag gtaaaccaat gttatcgtac gtatatgatt atccgcataa aaagctttat
     2341 aaagcaatac gaggagaagg tgctttttgt aatggaatta agatggaaga accaccatcg
     2401 ttaaaattgg aagacgctat tatatcgttt aatgcacaag tgatgaatct agatacggtg
     2461 caagatttat ttgacgcctc atttagttat cgtttagttg gtgcttgcgg attagattct
     2521 atgcgagtgg caaaaggtca atttggggca catattaata caaatcctaa accttgggac
     2581 attgctgcac agtttttatt tgcagaacta ttgaatctaa aaatgacgac tttagatggt
     2641 aaggcaattg atcatttaaa gggtgcacct tttattataa gtaataaagc atgtcatgaa
     2701 acggtactta aaattttaaa tgcaaatggt ggttatcaaa aatacagata gatattctct
     2761 agagataaaa aatgataaaa aatagttata cgtctacaga agtatagtaa aaaaattatt
     2821 ttactatata ataaatagag aaaacagaca aagtatgtaa ggaatagtaa aggagtggtg
     2881 ggattgaaac gttcgtctaa aagtaaaatg agcctaccga aaaaaatatt tttatgggta
     2941 ttcggtattt tagtaatctt agcgattgta gctgttgtgt atgtagctgc taaaattttt
     3001 attactggta ataagataca caatccgtta gatcgcaatc attcagaatt aagagataaa
     3061 aaagtgagtt taaacgatgg tgatccattc acaatcgctt tatttggtgt cgattcagat
     3121 gctgatcgta agaaaaaggg tggcggagag cgtagtgata gtattatgat tttatctatc
     3181 aaccctaaaa cgaagaaaac tgaaatagta agtataccgc gtgatacaag agcggaaatt
     3241 gttggccgtg gtactactga aaaaattgca catgcctatg catatggtgg accaaatatg
     3301 gctgttaaat cacttgaaaa attaatgaat gtgccaattg atcattatgc gacaatcgat
     3361 atggatggtt tgcataatat gatagatagc atcggcggtg ttgatgtagt aagtaatgat
     3421 acattcactg ttgatggtgt tcgttttaca aaaggacagc agacacatgt aaatggtgat
     3481 caagcattga agtttattag aagtcgtaaa gaagaaggtg ctggtggtga ctttggtcga
     3541 caacaacgtc agcaaattgt gcttgaagcg atggccaata agattgcaag cccatcatcg
     3601 attacacatt ttaatagtct aatgaatgaa attcaaaata atgttaaaac agatttaaca
     3661 ttaggtgatt tgaatacgat tagaagcaat tataaagatg ctaatgacac gattaataaa
     3721 catcaattga gtggtcaagg cggcattcaa agtgacggat tgtactactt cattcctagt
     3781 gagcagtcaa aagctgaaag tactaagtta ttaaaagata atctagagta gtaaactaag
     3841 ggaaaataag ttatcaaaat tgtacacaaa ttaattagat agaatgatct gtaaatagaa
     3901 taacgaatag aactaatagc ccgtaaatag aaggtgataa cactttgtat ttatgggcta
     3961 ttttatttaa tgcctgattt gatttttata aattaa
//
LOCUS       DAHHSC010000207         3971 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.208, whole genome shotgun sequence.
ACCESSION   DAHHSC010000207 DAHHSC010000000
VERSION     DAHHSC010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3971)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3971)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3971
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.208"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            complement(<1..527)
                     /locus_tag="NBI57_002317"
     CDS             complement(<1..527)
                     /locus_tag="NBI57_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000776424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HCD1823245.1"
                     /translation="MKLQWINTFKLHSKKRQLSKAQQIDLLSNLCNLLKYGFTLYQSF
                     QFLNLQMTYKNKQLGTTILSEISNGAPCNQILSLIGYSDTIVMQVYLAERFGNIIDVL
                     EETVNYMKVNRKSEQRLLKTLQYPLILVSIFIAMIIILNLTVIPQFQQLYTSMNIQLS
                     SFQKTLSFFITSLPT"
     gene            complement(499..1473)
                     /locus_tag="NBI57_002318"
     CDS             complement(499..1473)
                     /locus_tag="NBI57_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II/IV secretion system protein"
                     /protein_id="HCD1823246.1"
                     /translation="MKILFQEIINKAIEMKASDVHFIPVKNEVSIKFRINDNLEQYEQ
                     IGNSIYQKLLVYMKFQAGLDVSTQQVAQSGRYSYHFNKIYFLRISTLPLSLGQESCVI
                     RIVPQFFQQQKSTYKFNDFKHLMNKKQGLLLFSGPTGSGKSTLMYQMVSYANKALNLN
                     VISIEDPVEMQIPGIVQINVNDKAGINYVNSFKAILRCDPDVILIGEIRDKDVAKCVI
                     QASLSGHLVLTTLHATDCKGAILRLLEMGISVQELIQATNLIINQRLVTTIKQQRQLV
                     CEILSQQQLRYFFSHNHSLPSSFKNLEDKLDDMTKAGVICETTMDKYI"
     gene            complement(1525..2148)
                     /locus_tag="NBI57_002319"
     CDS             complement(1525..2148)
                     /locus_tag="NBI57_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HCD1823247.1"
                     /translation="MRISSLTLGLVDTNTYFIENDKAVILIDPSGESEKIIKKLNQIN
                     KPLKAILLTHAHFDHIGAVDDIVDRFDVPVYMHEAEFDFLKDPVKNGADKFKQYGLPI
                     ITSKVTPEKLNEGSTEIEGFKFNVLHTPGHSPGSLTYVFDEFAVVGDTLFNNGIGRTD
                     LYKGDYETLVDSIQDKIFELEGDLPLFPGHGPYTTVDDEQLNPFLHG"
     gene            complement(2145..2474)
                     /locus_tag="NBI57_002320"
     CDS             complement(2145..2474)
                     /locus_tag="NBI57_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MTH1187 family thiamine-binding protein"
                     /protein_id="HCD1823248.1"
                     /translation="MAIVDVVVIPVGTEGPSVSKYIADIQKKLQEYKAMGKIDFQLTP
                     MNTLIEGELSDVLEVVQVIHELPFDKGLSRVCTNIRIDDRRDKSRKMNDKLTSVQKHL
                     ENSGENL"
     gene            complement(2474..3460)
                     /locus_tag="NBI57_002321"
     CDS             complement(2474..3460)
                     /locus_tag="NBI57_002321"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HCD1823249.1"
                     /translation="MTKIILAADVGGTTCKLGIFTPELEQLHKWSIHTDTSDSTGYTL
                     LKGIYDSFVEKVNENNYNFSNVLGVGIGVPGPVDFEKGTVNGAVNLYWPEKVNVREIF
                     EQFVDCPVYVDNDANIAALGEKHKGAGEGADDVVAITLGTGLGGGIISNGEIVHGHNG
                     SGAEIGHFRADFDQRFKCNCGRSGCIETVASATGVVNLINFYYPKLTFRSSILELIKE
                     NKVTAKAVFDAAKAGDQFCIFITEKVANYIGYLCSIISVTSNPKYIVLGGGMSTAGPI
                     LIENIKTEYHNLTFAPAQFETEIVQAKLGNDAGITGAAGLIKTYVLDKEGVK"
     gene            complement(3457..3660)
                     /locus_tag="NBI57_002322"
     CDS             complement(3457..3660)
                     /locus_tag="NBI57_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HCD1823250.1"
                     /translation="MSRKINNFYDVQQLLKSYGFLIYFKNPEDMYEMIQQEISSLYQY
                     ELLSKEEYLKCTLIINQRRNEQK"
     gene            complement(3641..>3971)
                     /locus_tag="NBI57_002323"
     CDS             complement(3641..>3971)
                     /locus_tag="NBI57_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001652331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1823251.1"
                     /translation="TSGNYDNAQNIVKQTINKNYADDQTYYLSGMIMATINSKSEGMT
                     EWERGLRMFPKSGLLNFELAIANRSLNDDEKALKYVRKALNADPKNTDYINLEKELTK
                     SNESKNK"
BASE COUNT         1266 a          721 c          537 g         1447 t
ORIGIN      
        1 atagtaggta agctggtaat gaaaaaagac aatgtttttt gaaaagaaga tagttgaata
       61 ttcatagaag tatataattg ttgaaactgt ggaattactg tgaggtttaa tataataatc
      121 atagcaataa agatagaaac tagtattaag gggtattgca gtgtctttaa caatcgttgt
      181 tctgactttc tattcacttt catataattt acggtttctt ctagaacgtc tataatattg
      241 ccaaatcttt ctgccaaata tacttgcatg acgatagtat cgctataacc tatcagtgat
      301 aatatttgat tgcatggtgc accatttgaa atttcactta gaatggtggt acctaattgc
      361 ttatttttat atgtcatttg aagatttaaa aattgaaaac tttgatacag agtgaaacca
      421 tatttcaaca aattacataa atttgaaagt aaatcgattt gttgggcctt acttaattgt
      481 cgcttcttag aatgtagttt aaatgtattt atccattgta gtttcacaaa tgacacctgc
      541 ttttgtcata tcatcaagtt tatcttctaa gttcttaaat gatgatggta atgaatgatt
      601 atgggaaaag aaatatcgga gttgttgctg agatagaatt tcgcatacta attgtcgctg
      661 ttgcttaata gtagttacaa gtcgttggtt tataattaag ttagttgcct gtatcaattc
      721 ttgtacagaa atgcccattt ctaatagcct taaaatagca cctttacaat cagttgcatg
      781 caatgtagtc agaacaaggt gaccacttaa actagcctgt ataacacact tggcaacatc
      841 tttatctctg atttcaccta ttaaaataac atcaggatca catcttaaaa tagctttaaa
      901 cgaatttaca tagttaatgc cagctttatc attcacatta atttggacga taccaggaat
      961 ttgcatctct acaggatcct ctatagaaat tacatttaaa ttcaaggctt tattcgcgta
     1021 tgagaccatt tgatacatta atgtactctt tcctgaacca gttggcccac taaacaatag
     1081 taatccttgt ttcttattca tgaggtgttt aaaatcattg aatttataag ttgatttctg
     1141 ttgttgaaaa aattgaggta caattctgat aacacaactt tcttggccaa gtgacaatgg
     1201 taaagttgat attctcaaaa aatatatttt attgaaatgg taactatatc gaccgctctg
     1261 tgcgacttgc tgtgtagaaa catcaagccc agcttgaaac ttcatataaa ctaataactt
     1321 ttgataaatg ctattcccaa tttgttcata ctgctccaag ttatcattaa ttctaaattt
     1381 aatacttact tcatttttaa ctggaataaa atgtacatca ctcgctttca tttctatcgc
     1441 tttattaatt atttcttgaa atagaatctt caaaaaaaca cctcctacat ataatcacgt
     1501 aggaggtgtt tttattactt caatttaacc gtgtaaaaat ggatttaatt gttcatcatc
     1561 aaccgtcgta tatggaccat gtccagggaa taaaggtaaa tcgccttcta attcaaatat
     1621 tttatcttga atagaatcaa ctagcgtttc ataatcacct ttatataaat ctgtacgtcc
     1681 gattccatta ttaaataatg tatctccaac aactgcgaat tcatcgaaca catatgttaa
     1741 acttcctggt gaatgtccag gtgtgtgtaa cacattaaac ttaaatcctt ctatttctgt
     1801 gctaccttcg tttaactttt caggagttac cttacttgta ataattggta atccatattg
     1861 cttaaattta tctgctccat ttttaacggg atcttttaga aaatcaaact ctgcttcatg
     1921 catataaacc gggacatcga atcgatcaac tatatcatcg actgctccga tatgatcaaa
     1981 gtgtgcatgt gttaataaaa tagcttttaa cggtttattt atttggttta attttttaat
     2041 aattttttca ctttcacctg aagggtcaat cagaataaca gctttgtcat tttcgatgaa
     2101 atacgtatta gtatcaacta agcctaaagt taagcttgaa atcctcatag gttttcacca
     2161 ctattttcta aatgtttttg tactgatgtt agtttatcat tcatttttct agatttgtct
     2221 cgtcggtcat caatacggat atttgtacaa actctactta aacctttatc aaaaggtaat
     2281 tcatgtatca cttgcacaac ttctaataca tcgcttaatt caccttcaat tagagtattc
     2341 attggtgtta attgaaaatc aattttaccc attgctttat attcttgaag ttttttctga
     2401 atatctgcaa tatatttact aacactcgga ccttccgttc caactggaat aacaaccaca
     2461 tcaacaatag ccattatttt accccctctt tatctaatac ataggtcttg attaatcctg
     2521 ctgctcctgt aatacctgca tcattaccta atttcgcttg tacaatttca gtttcaaatt
     2581 gagcaggtgc aaatgttaaa ttatgatatt ctgttttaat attttcaatt aaaataggtc
     2641 ctgcagtaga cattcctcca cctagaacga tatatttcgg attacttgta acactaataa
     2701 tactacataa atatccaata tagtttgcaa ccttttcagt aatgaaaata cagaattggt
     2761 caccagcttt tgccgcatca aaaacagctt ttgctgtaac cttattttct ttaatcaatt
     2821 ctaatataga agatctaaac gtcaacttcg gatagtagaa gttaattaag ttaacaacgc
     2881 ctgtcgctga agcaactgtt tcaatacatc cagaacgacc acaattacat ttaaatcgtt
     2941 gatcgaagtc tgctctaaaa tgacctattt ctgcgccaga gccattatga ccatgtacga
     3001 tttcaccatt tgaaataatt cctccaccta gacctgtacc aagtgtgatg gcaacaacat
     3061 catcggcacc ttcaccagca cctttgtgtt tctcccctaa agcagctatg ttagcatcat
     3121 tatctacata cactggacaa tcaacgaatt gttcaaaaat ctcacgtaca ttaacttttt
     3181 ctggccaata taagtttact gctccattta ctgtaccttt ttcaaagtca acaggacctg
     3241 gtacaccaat acctacgcca agtacatttg aaaaattata attattttca tttacttttt
     3301 caacaaacga atcataaatt cctttcaaaa gtgtatatcc tgtactatca gatgtatcag
     3361 tgtgaataga ccatttatgt aattgttcta attcaggtgt gaaaatacct aatttacaag
     3421 tcgtcccgcc tacatcagct gctaaaataa tttttgtcat ttctgttcat tccttctctg
     3481 attaattatc aacgtacatt tcaaatattc ttccttagat aacagttcat attgatacaa
     3541 tgatgaaatc tcctgttgaa tcatttcgta catatcttct ggatttttaa aatatattag
     3601 aaatccgtaa cttttcaata actgttgtac atcataaaag ttatttattt ttcgactcat
     3661 ttgatttagt caactctttt tctaagttaa tataatctgt atttttaggg tctgcattta
     3721 atgctttacg cacatatttt aatgcttttt catcatcatt taatgaacga tttgctatcg
     3781 ctaactcaaa atttaataaa cctgatttag gaaacattct aagtcctcgc tcccattctg
     3841 tcataccttc ggacttagaa ttgatagtag ccataatcat accacttaaa taatatgttt
     3901 gatcatcggc ataattttta tttattgtct gcttcacaat attttgagca ttatcataat
     3961 taccactagt c
//
LOCUS       DAHHSC010000208         3965 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.209, whole genome shotgun sequence.
ACCESSION   DAHHSC010000208 DAHHSC010000000
VERSION     DAHHSC010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3965)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3965)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3965
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.209"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            complement(<1..175)
                     /locus_tag="NBI57_002324"
     CDS             complement(<1..175)
                     /locus_tag="NBI57_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001251209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsK"
                     /protein_id="HCD1823252.1"
                     /translation="MAMNEIALFKGVRIQPYQKYIDFMFASVLAFIFLVYRIYILFID
                     YKSVNKKLDILTLF"
     gene            complement(229..2724)
                     /locus_tag="NBI57_002325"
     CDS             complement(229..2724)
                     /locus_tag="NBI57_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HCD1823253.1"
                     /translation="MNLKAPYAGLRDNLLLTKTGEVWAYYRVRSESISTANYDAKEES
                     KKRMRYFLESIKGYQDFHFEMYDHDLDLRNKFKEISKDFDDEAFNVAEYYSKETIDVL
                     EQELQMITHNSFVMGVKIRELADDAVTLKEILKSTLSDTAERIVSNFGFDVNLDDKIL
                     DKYKTFERDLADSFLGIGGERLTENELAYIIRKPFISNATHDVQEESSRRAITDIYNA
                     VIDPTRLSVLEVENDNEKFYTTTVVVDDFPLDMEYTHLFEKAQNMPFPVECHVKAKII
                     NNEKVKKKFDRAKINHKQQAKEKQDTGDSASEDVQDNLFVLNQMEREVTGSGVFIEWV
                     FMFVIKSDDYRDCKRKAKRLMKRLKETQIYAVNPLADQLQLFYQCLHGNDLFINKYWL
                     QRTTATGIAENLFGVSQSLGTKTGFYIGRIDKFIRSVSREEAVASSRDIILFSLLLAA
                     KGIKGAVSDSPHVLITGQTGKGKSFLAKLLWIYTSFFKGQMLYWDPKSEFAEWFDRVT
                     ESKEMQEKYPLFINHLKTFKYVTLNYEDSTNYGCLDPIVFLDGIEANEMLKSVFDEIK
                     SFENYHHIETAIYQAISDTVEERENGKKVGSLNVIEKLQNNEDEHVKNAGDLLFEKTQ
                     NNILKLVFSDGTNPALNIQEKATILQVKGLDMPKADDDTSSYSTSEKNGITLMLLIGK
                     FLEKFGSRRDVQTTIFIDEGWAFSASRQGKKVGKRIKRTGRSENNSLVFITQSVKDKA
                     DDDGGNFGCHFAFDEKDEREDILKSLGLEYSKESPENMEMLKDLKKGQCIFSDFYGRV
                     GKMVVHCPFEEMTEAFRTQEDSASSKAEEKFAM"
     gene            complement(2759..3142)
                     /locus_tag="NBI57_002326"
     CDS             complement(2759..3142)
                     /locus_tag="NBI57_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015581841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HCD1823254.1"
                     /translation="MDKKAYNLKRTFEQPIVMYEFTDKFRINKGFRLDFWATFLIVWF
                     ILFLLFWYLLQPVIMSIGGLMFIYFTVAPYYITKYIVKLKQDGKKLFFFLWDFVIFVF
                     NVQLRKVKLSYDEEVEYHDKKITFK"
     gene            complement(3154..3414)
                     /locus_tag="NBI57_002327"
     CDS             complement(3154..3414)
                     /locus_tag="NBI57_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000015642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1823255.1"
                     /translation="MSGLFNFFVLGADRPSLGGVGDWISNEVGTGIGLVVLVVGIVKW
                     ASGKYGHMVILFVVGGFLFLVSKGPEQVFNAIAGVWKMIFGG"
     gene            complement(3419..>3965)
                     /locus_tag="NBI57_002328"
     CDS             complement(3419..>3965)
                     /locus_tag="NBI57_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000691999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HCD1823256.1"
                     /translation="NITEKKEVKVKKKKKSEKDKDEYETKTEEKQRKVNQNILINIPI
                     KSENNKYVVVEYPYFTPIPDSQLNKAKMVEDNLKDNKREDNPKAKAFIEDFFNKYASS
                     KSDDMAYLMDNPEGLEGTREVSQIREIRLYPKGDDYVAKVEILMKDKDSPLENLEHYT
                     LDITKKDGKYYVKNMTNSIGG"
BASE COUNT         1360 a          800 c          393 g         1412 t
ORIGIN      
        1 caaacaacgt caatatatcc aattttttat taactgattt ataatcaata aacaaaatgt
       61 atatacgata taccaaaaaa ataaatgcta atacacttgc aaacataaaa tcaatatact
      121 tctgataagg ctgtatacgt acgcctttaa ataaagcaat ttcattcatt gccatcaaat
      181 ccactccttt aaaataaaaa gcacatacaa ttttgtatgt gctaaaaatt acatcgcaaa
      241 tttttcttca gctttagagc ttgcagaatc ttcttgtgtt ctaaatgctt cagtcatttc
      301 ctcaaatgga caatgtacaa ccatttttcc aacacgtcca taaaaatcac tgaatataca
      361 ttgacctttc ttcaaatctt tcaacatctc catattttca ggagattctt ttgaatattc
      421 taagcctaaa gattttaaaa tatcctcacg ttcatctttt tcatcaaatg caaaatgaca
      481 accaaagtta ccaccatcat catctgcttt atcttttacc gattgtgtaa taaatacaag
      541 tgaattattc tcagaacgtc ccgttctttt tatacgctta ccaacttttt taccttgtct
      601 tgaagcacta aaagcccaac cctcatcaat aaatatagtc gtttgaacat ctctacgaga
      661 accgaatttc tctaaaaact taccaattaa taacattaat gtaatcccat ttttctcact
      721 tgttgaataa cttgatgtat catcatctgc tttaggcata tctaaacctt taacctgtaa
      781 aatagttgct ttttcttgaa tatttaaagc tggattcgta ccatcactaa aaacaagttt
      841 taaaatattg ttttgcgttt tttcaaataa taaatcgcct gcatttttta catgctcatc
      901 ttcattattc tgtaattttt caattacatt taatgaacca acttttttac cattttcacg
      961 ttcttcaacc gtatcagaaa tagcttgata aattgctgtt tcaatatgat gataattttc
     1021 aaaagattta atttcatcaa acactgattt caacatctca ttagcttcaa taccatctaa
     1081 aaatacaata gggtctagac aaccataatt ggttgaatct tcataattca atgttacata
     1141 tttaaatgtt tttaaatgat taataaatag gggatacttt tcttgcattt ccttactttc
     1201 tgttactctg tcaaaccatt ctgcaaactc agattttgga tcccagtaca acatttgacc
     1261 tttaaaaaat gatgtataaa tccataataa ttttgctaaa aatgatttac ctttaccagt
     1321 ttgaccagta attaaaacat gcggactatc actgacagcc cccttaatac cttttgcggc
     1381 caacaataaa ctaaacaaaa ttatatccct tgaacttgca acagcttcct cacgtgaaac
     1441 agaacgtata aatttatcaa tacgccctat ataaaaaccc gtttttgttc ccaaactttg
     1501 agacacacca aataaattct ctgctatacc tgttgccgtt gttctttgta accaatactt
     1561 attaataaat aaatcattac catgtaaaca ttgataaaac aattgtaact ggtcagctaa
     1621 tggattaact gcatatattt gagtttcctt taatcgtttc atcaaacgct ttgctttacg
     1681 tttacaatct ctataatcat cacttttaat tacaaacata aatacccact caataaatac
     1741 acctgagcca gtcacttctc gttccatttg attcaataca aacaaattat cttgaacatc
     1801 ttccgaagca ctgtcgccag tatcttgctt ttcttttgct tgttgtttat gatttatttt
     1861 cgcacggtca aattttttct taactttctc attattaatg atttttgctt ttacatggca
     1921 ttcaaccgga aaaggcatat tttgagcttt ctcaaataaa tgtgtatatt ccatatctaa
     1981 cggaaaatca tcaacaacaa ctgttgttgt ataaaatttc tcgttatcat tttcaacttc
     2041 taaaacactc aagcgagtag ggtcaattac tgcattataa atatctgtaa tagctcttct
     2101 tgaagattct tcctgtacgt catgtgttgc atttgaaata aaaggctttc taataatata
     2161 agccaattca ttctcagtta aacgctcgcc tccaatacct agaaaactat cagccaaatc
     2221 tcgttcaaat gttttatact tatctaaaat tttatcgtct aaattcacat caaaaccaaa
     2281 attcgaaaca atacgctcag ccgtatctga taatgtactt tttaaaatct ctttaagtgt
     2341 aactgcatcg tcagctaatt cacgtatttt aacacccata acaaaagaat tatgcgtaat
     2401 catttgtaat tcttgttcta atacgtcaat agtttctttt gaataatatt cagctacatt
     2461 aaaagcttca tcatcaaaat ctttactaat ttctttaaat ttatttcgca aatctaaatc
     2521 gtggtcatac atttcaaaat gaaaatcttg ataaccttta atcgactcta aaaaataacg
     2581 catacgcttt tttgattctt cttttgcatc ataatttgct gttgatatag actcagaacg
     2641 aacacgataa tacgcccaaa cttcgccagt tttcgttaat aacaaattat ctcttaaacc
     2701 tgcatacggt gcttttaaat tcatgtcatc cctccaataa aaataacccc aataggggct
     2761 atttaaatgt tattttttta tcatgatatt ctacttcttc atcataactt aatttaactt
     2821 ttcttaactg cacattaaaa acaaatatta caaaatccca taagaagaaa aataatttct
     2881 ttccatcttg ttttaactta acaatatatt tcgttatata ataaggtgca accgtaaagt
     2941 aaataaacat aagtccacca atcgacataa ttacaggctg taataaatac caaaataaca
     3001 aaaataatat aaaccacaca atcaaaaatg tagcccaaaa atctaaacga aaacccttat
     3061 taatacgaaa cttatctgta aattcataca taactatcgg ttgttcaaaa gttcgcttta
     3121 aattataagc ttttttatcc aaatggaatc acctcaaccg ccaaaaatca ttttccaaac
     3181 acctgcaatc gcattaaata cctgttcagg acctttagaa actaagaata aaaagccacc
     3241 aacaacaaat aatataacca tatgcccata tttaccacta gcccatttta caataccaac
     3301 aaccaatact actaaaccaa tacccgtacc tacttcatta cttatccagt caccaactcc
     3361 accaagtgat ggacggtcag cacctaaaac aaaaaaatta aataaaccac tcataacatt
     3421 aacctccaat agaattagtc atatttttaa cgtaatattt accatctttt tttgttatat
     3481 ctaatgtgta atgctccaaa ttctccaaag gagaatcttt atctttcatc aaaatttcaa
     3541 ctttagcaac ataatcatcg ccttttggat ataacctaat ttctcttatc tgagaaactt
     3601 ctctagttcc ttctaagccc tcagggttat ccattaaata ggccatatca tcagacttac
     3661 tagaagcata tttattaaag aaatcttcaa taaaagcttt tgctttcgga ttgtcttccc
     3721 gtttattgtc tttcaaatta tcctcaacca ttttagcttt attcaattga ctatcaggga
     3781 ttggcgtaaa ataaggatat tcaacaacaa catacttatt attttcactc ttaataggta
     3841 tatttattaa aatgttttga tttactttac gttgcttttc ttctgttttt gtttcatact
     3901 catctttatc tttttcactt ttctttttct tcttaacttt aacttctttc ttttcagtaa
     3961 tattc
//
LOCUS       DAHHSC010000209         3963 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.210, whole genome shotgun sequence.
ACCESSION   DAHHSC010000209 DAHHSC010000000
VERSION     DAHHSC010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3963)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3963)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3963
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.210"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            complement(16..231)
                     /locus_tag="NBI57_002329"
     CDS             complement(16..231)
                     /locus_tag="NBI57_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1823257.1"
                     /translation="MSINIDPEKFAELVVMSNPSKFEDAEDIAKESLKLYINAYRLAE
                     RYSTIATNCYDTAEVIKELKKTDLQLK"
     gene            405..1202
                     /locus_tag="NBI57_002330"
     CDS             405..1202
                     /locus_tag="NBI57_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HCD1823258.1"
                     /translation="MRIMFIGDIVGKIGRDAIETYIPQLKQKYKPTVTIVNAENAAHG
                     KGLTEKIYKQLLRNGVDFMTMGNHTYGQREIYDFIDEAKRLVRPANFPDEAPGIGMRF
                     IQINDIKLAVINLQGRAFMPDIDDPFKKADQLVKEAQEQTPFIFVDFHAETTSEKYAM
                     GWHLDGRASAVVGTHTHIQTADERILPKGTGYITDVGMTGFYDGILGINKTEVIERFI
                     TSLPQRHVVPNEGRSVLSGVVIDLDKEGKTKHIERILINDDHPFSTF"
     gene            1342..3102
                     /locus_tag="NBI57_002331"
     CDS             1342..3102
                     /locus_tag="NBI57_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HCD1823259.1"
                     /translation="MKPQLSWKVGGQQGEGIESTGEIFATAMNRKGYYLYGYRHFSSR
                     IKGGHTNNKIRVSTTPVHAISDDLDILIAFDQETIDVNHHEMREDSIILADAKAKPVK
                     PEGCHAQLIELPFTATAKELGTALMKNMVAIGATSALMNLNTNTFEELITNMFSKKGD
                     KVVEVNIQALNEGYQLMQSRLPEIDGDFELESTDALPHLYMIGNDAIGLGAIAAGSQF
                     MAAYPITPASEVMEYMIANISKVNGAVIQTEDEIAAVTMAIGANYGGVRAFTASAGPG
                     LSLMMEAIGLSGMTETPLVIINTQRGGPSTGLPTKQEQSDLMQMIYGTHGDIPKIVVA
                     PTDAEDAFYLTMEAFNLAEQYQCPVIVLSDLQLSLGKQTVEKLDYNRIEIKRGEIIQS
                     DIEREEDDKGYFKRYALTSNGVSPRPIPGVKGGIHHITGVEHNEEGKPSESASNRQQQ
                     MEKRMRKIEQLLIESPVEANLQHEDADILYIGFISTKGAIQEGSNRLNQQGIKVNTIQ
                     IRQLHPFPTSVIQDAVNKAKKVVVVEHNYQGQLASIIKMNVNIHDKIENYTKYDGTPF
                     LPHEIEEKGKIIATEIKEMV"
     gene            3103..>3963
                     /locus_tag="NBI57_002332"
     CDS             3103..>3963
                     /locus_tag="NBI57_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HCD1823260.1"
                     /translation="MATFKDFRNNVKPNWCPGCGDFSVQAAIQKAAANIGLEPEEVAI
                     ITGIGCSGRLSGYINSYGVHSIHGRALPLAQGVKMANKDLTVIASGGDGDGYAIGMGH
                     TIHALRRNMNMTYIVMDNQIYGLTKGQTSPSSAVGFVTKTTPKGNIEKNVAPLELALS
                     SGATFVAQGFSSDIKGLTKLIEDAINHDGFSFVNVFSPCVTYNKINTYDWFKEHLTSV
                     DDIENYDSTDKQLATKTVIEHESLVTGIVYQDKETPSYESQIKELDDTPLAKRDIKIT
                     EDTFNALTEQF"
BASE COUNT         1420 a          582 c          751 g         1210 t
ORIGIN      
        1 tatttctaag ttgagttatt ttaattgtaa atctgttttc tttaattctt ttataacttc
       61 tgcagtatca taacaatttg ttgcaattgt tgaatatctc tctgctaaac gatatgcatt
      121 aatgtaaagc tttaaacttt ctttagctat atcctctgca tcttcgaatt ttgatgggtt
      181 agacataacc actaattctg caaatttttc tggatcaata ttaatagaca tgtatttatt
      241 tacaactcct atttattttg atgtcttaat actaacatat tgaagttttc agacaaagta
      301 atgtctctct ataattgaag aaaaataatt caaagaattt aatatttact ttaaatatta
      361 gaacgtatga attaaactag tatttatgag aggatgaaca aaacatgaga ataatgttta
      421 taggggatat cgtaggtaaa attggacgag acgcaattga aacgtacata cctcaactga
      481 agcaaaagta taaaccaaca gttacaattg taaatgctga aaatgcagca catggtaaag
      541 gtttgactga aaaaatatat aaacaattac taagaaatgg tgtagatttc atgactatgg
      601 gtaatcacac atatggtcaa cgtgaaattt atgattttat agatgaagca aaacgactag
      661 taagaccagc gaattttccg gatgaagcgc cgggaattgg tatgagattt atacaaatta
      721 atgatattaa acttgcagtt attaatctgc aaggaagagc gtttatgcca gatattgatg
      781 atccttttaa aaaggcagat caattagtca aggaagcaca agaacaaact ccgtttatat
      841 ttgttgattt tcatgcagaa acaacttctg aaaagtatgc aatgggatgg catttagatg
      901 gtagagctag cgctgttgtt ggaacgcata cacacattca aacagcagat gaacgtattt
      961 taccaaaggg gacagggtat ataacggatg ttggtatgac aggtttttat gacggcattt
     1021 taggaataaa taaaacagag gtaattgagc gttttatcac tagtttgcca caaagacatg
     1081 ttgttccaaa tgaaggtaga agtgtattat ctggtgttgt tattgattta gacaaagaag
     1141 gtaaaacaaa gcacatcgaa cgtatattga taaatgatga ccatccattt tcaacatttt
     1201 aaaattacgt aagtaaacat tcgaattgga ccctatcgtc cattagtatg aatttaatat
     1261 agtaccactg tttacatagt aaatcggtgg ttctttttgt tatcatttaa tatgaaatat
     1321 atccatagga ggcatataac tatgaaacca caattatcgt ggaaagttgg cggtcaacaa
     1381 ggcgaaggta ttgaatcaac tggggaaatc ttcgctacgg ctatgaatag aaaaggatat
     1441 tatttatatg gatatagaca tttttcaagt cgtatcaaag gtggacatac gaataataaa
     1501 attagagttt ctacgacgcc tgttcatgca attagtgatg atttagatat tttgattgca
     1561 tttgaccaag aaacaattga tgttaaccat catgaaatga gagaagacag tattatttta
     1621 gctgatgcca aggctaaacc tgtgaaacca gaaggatgtc atgcacagct tattgaatta
     1681 ccttttacag caaccgctaa agaattaggt acagcattaa tgaaaaacat ggttgcaata
     1741 ggtgctacta gcgcattgat gaatttgaat acaaatacat ttgaagaact tattactaat
     1801 atgttttcta aaaaaggtga caaggtagtt gaagtcaata tccaagcatt aaacgaaggt
     1861 tatcaattaa tgcaatcccg cttacctgaa atcgacgggg actttgaatt agagtcaaca
     1921 gatgcactac cacatctata tatgattggt aacgatgcca ttggattagg tgcaattgct
     1981 gcaggttcac aatttatggc ggcatatcct attacacctg cgtctgaagt tatggaatat
     2041 atgattgcca atatatctaa agtaaacgga gcggttattc aaacagaaga tgaaattgct
     2101 gctgtaacta tggctattgg tgcaaattat ggtggagtca gagcgtttac ggctagtgct
     2161 ggtccaggtt tatctttaat gatggaagca attggattat ctggtatgac tgaaacgcca
     2221 ttagtcatta ttaatactca acgaggtgga ccttctactg gattacctac gaaacaagaa
     2281 cagtcagatt taatgcaaat gatttatggt acacatggtg atattccaaa aatagttgta
     2341 gcaccaactg atgcagaaga tgcattttat ttaactatgg aagcctttaa tttagcagaa
     2401 caatatcaat gccctgttat agttttaagt gatttgcaat tatctttagg taaacaaact
     2461 gttgaaaaat tagattataa tcgcattgaa attaaacgtg gtgaaatcat tcaatctgat
     2521 attgaacgtg aagaagatga taaaggttat ttcaagcgtt atgcgttaac atccaatggt
     2581 gtttctccta gacctatccc cggtgttaaa ggaggtattc atcatataac tggtgtggaa
     2641 cacaatgaag aaggtaaacc tagtgaatct gcgtcaaata gacaacaaca aatggaaaaa
     2701 cgaatgcgta aaattgagca gttactaatt gaatcgccag tagaagctaa cttacaacat
     2761 gaggatgcag atattcttta tatcggtttt atttctacaa aaggtgcaat tcaagaaggt
     2821 agtaaccgtt tgaatcaaca aggcataaaa gttaacacta tacaaattag acaattgcat
     2881 ccattcccaa caagcgttat tcaagatgca gttaataaag cgaagaaagt cgttgtagtg
     2941 gagcacaatt atcaaggaca attggctagt attataaaaa tgaatgtcaa tattcatgat
     3001 aagattgaaa attatacaaa gtatgatggg acacctttcc taccacatga aatcgaagaa
     3061 aaaggcaaaa taattgctac tgaaataaag gagatggtat agatggcgac atttaaagat
     3121 tttagaaata atgttaagcc taactggtgc cccggatgtg gcgatttctc agtacaagct
     3181 gcaattcaaa aagcagccgc aaatataggg ttagaacctg aagaagtagc tatcatcacc
     3241 ggtataggat gttctggccg tctttcagga tatattaatt cttatggcgt tcattctatt
     3301 cacggacgtg cattaccttt agctcaaggt gtaaaaatgg cgaataaaga tttaactgtt
     3361 attgcatcgg gaggagatgg tgatggttat gctataggta tggggcatac aatccatgct
     3421 ttaagaagaa atatgaacat gacgtatata gtcatggata atcaaattta tggtttgaca
     3481 aagggacaaa catcgccgtc atcagcagta ggatttgtta ctaaaacaac gccaaaaggt
     3541 aatatagaaa aaaatgttgc gcctttagaa ttagcattat catctggtgc cacatttgta
     3601 gcccaaggtt tttcaagcga tattaaagga ttaacaaaac taattgaaga tgcaattaat
     3661 catgatggat tttcattcgt taatgtcttt tcaccatgtg tgacttataa taaaattaac
     3721 acatacgatt ggtttaaaga acatttaaca agtgttgatg acattgaaaa ttatgattct
     3781 acagataaac aattagcgac taaaactgtt attgaacatg aatctttagt aactggtatt
     3841 gtttatcaag ataaagaaac accatcatat gaatctcaaa ttaaagagtt agatgataca
     3901 ccacttgcta aaagagatat caaaattact gaagacacct tcaatgcatt aactgaacaa
     3961 ttt
//
LOCUS       DAHHSC010000210         3918 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.211, whole genome shotgun sequence.
ACCESSION   DAHHSC010000210 DAHHSC010000000
VERSION     DAHHSC010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3918)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3918)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3918
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.211"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            267..1187
                     /gene="mvk"
                     /locus_tag="NBI57_002333"
     CDS             267..1187
                     /gene="mvk"
                     /locus_tag="NBI57_002333"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HCD1823261.1"
                     /translation="MTRKGYGESTGKIILIGEHAVTFGEPAIAVPFNAGKIKVLIEAL
                     ESGNYSSIKSDVYDGMLYDAPDHLKSLVNRFVELNNITEPLAVTIQTNLPPSRGLGSS
                     AAVAVAFVRASYDFLGKSLTKEELIEKANWAEQIAHGKPSGIDTQTIVSGKPVWFQKG
                     HAETLKTLSLDGYMVVIDTGVKGSTRQAVEDVHKLCEDPQYMSHVKHIGKLVLRASDV
                     IEHHNFEALADIFNECHADLKALTVSHDKIEQLMKIGKENGAIAGKLTGAGRGGSMLL
                     LAKDLPTAKNIVKAVEKAGAAHTWIENLGG"
     gene            1192..2175
                     /gene="mvaD"
                     /locus_tag="NBI57_002334"
     CDS             1192..2175
                     /gene="mvaD"
                     /locus_tag="NBI57_002334"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HCD1823262.1"
                     /translation="MIKSGKARAHTNIALIKYWGKKDEALIIPMNNSISVTLEKFYTE
                     TKVTFNDQLTQDQFWLNGEKVSGKELEKISKYMDIVRNRAGIDWYAEIESDNFVPTAA
                     GLASSASAYAALAAACNQALDLQLSDKDLSRLARIGSGSASRSIYGGFAEWEKGYNDE
                     TSYAVPLESNHFEDDLAMIFVVINQHSKKVPSRYGMSLTRNTSRFYQYWLDHIDEDLA
                     EAKAAIQDKDFKRLGEVIEENGLRMHATNLGSTPPFTYLVQESYDVMALVHECREAGY
                     PCYFTMDAGPNVKILVEKKNKQQIIDKLLTQFDNNQIIDSDIIATGIEIIE"
     gene            2188..3264
                     /locus_tag="NBI57_002335"
     CDS             2188..3264
                     /locus_tag="NBI57_002335"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HCD1823263.1"
                     /translation="MIQVKAPGKLYIAGEYAVTEPGYKSVLIALDRFVTATIEEADQY
                     KGTIHSKALHHNPVTFSRDEDSIVISDPHAAKQLNYVVTAIEIFEQYAKSCDIAMKHF
                     HLTIDSNLDDSNGHKYGLGSSAAVLVSVIKVLNEFYDMKLSNLYIYKLAVIANMKLQS
                     LSSCGDIAVSVYSGWLAYSTFDHEWVKHQIEDTTVEEVLIKNWPGLHIEPLQAPENME
                     VLIGWTGSPASSPHFVSEVKRLKSDPSFYGDFLEDSHRCVEKLIHAFKTNNIKGVQKM
                     VRQNRTIIQRMDKEATVDIETEKLKYLCDIAEKYHGASKTSGAGGGDCGITIINKDVD
                     KEKIYDEWTKHGIKPLKFNIYHGQ"
     gene            3441..3782
                     /locus_tag="NBI57_002336"
     CDS             3441..3782
                     /locus_tag="NBI57_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1450 domain-containing protein"
                     /protein_id="HCD1823264.1"
                     /translation="MKNTFLICDECQAVNIRTLQKKLEKLDPDAEIVIGCQSYCGPGR
                     RKTFTFVNNRPLAALTEEELIEKVSQQLKKPRDPEEEERLRKRHEERKRRKEEQDRKL
                     KEKLEKRKAQQ"
BASE COUNT         1424 a          540 c          821 g         1133 t
ORIGIN      
        1 gtagggctat tttataaaaa gataaagaat ctgaaatatc actcgataag atttaaactt
       61 tgaagtaaac gaaagttagt tttatactaa tcaagttaag tcaaatataa attcaaaata
      121 aagattatac ataatatgaa taatgtgaga taaagactgc aatgaaattt aacgcgaatg
      181 cgtatgcaac ttagaatatg atgtctttta ctaataaata aaaataatga atcaagtata
      241 ggataaatat aaaaggtgat attgacatga caagaaaagg atatggggaa tcgacaggta
      301 agattatttt aataggagaa catgctgtta catttggaga gcctgctatt gcagtaccgt
      361 ttaacgcagg taaaatcaaa gttttaatag aagccttaga gagcgggaac tattcgtcta
      421 ttaaaagcga tgtttacgat ggtatgttat atgatgcgcc tgaccatctt aagtctttgg
      481 tgaaccgttt tgtagaatta aataatatta cagagccgct agcagtaacg atccaaacga
      541 atttaccacc atcacgtgga ttaggatcga gtgcagctgt cgcggttgct tttgttcgtg
      601 caagttatga ttttttaggg aaatcattaa cgaaagaaga actcattgaa aaggctaatt
      661 gggcagagca aattgcacat ggtaaaccaa gtggtattga tacgcaaacg attgtatcag
      721 gcaaaccagt ttggttccaa aaaggtcatg ctgaaacatt gaaaacgtta agtttagacg
      781 gctatatggt tgttattgat actggtgtga aaggttcaac aagacaagcg gtagaagatg
      841 ttcataaact ttgtgaggat cctcagtaca tgtcacatgt aaaacatatc ggtaagttag
      901 ttttacgtgc gagtgatgtg attgaacatc ataactttga agccctagcg gatattttta
      961 atgaatgtca tgcggattta aaggcgttga cagttagtca tgataaaata gaacaattaa
     1021 tgaaaattgg taaagaaaat ggtgcgattg ctggaaaact tactggtgct ggtcgtggtg
     1081 gaagtatgtt attgcttgcc aaagatttac caacagcgaa aaatattgtg aaagctgtag
     1141 aaaaagctgg tgcagcacat acatggattg agaatttagg aggttaatgc gttgattaaa
     1201 agtggcaaag cacgtgcaca tacgaatatt gcacttataa aatattgggg taaaaaagat
     1261 gaagcactaa tcattccaat gaataatagc atatctgtta cattagaaaa attttacact
     1321 gaaacgaaag tcacttttaa cgaccagtta acacaggatc aattttggtt gaatggtgaa
     1381 aaggttagtg gcaaagaatt agagaaaatt tcaaaatata tggatattgt cagaaataga
     1441 gctggcatcg attggtatgc tgaaattgaa agcgacaatt ttgtaccaac agcagcaggg
     1501 ttggcttcat cagcaagcgc atatgcagct ttagcagcag cttgtaatca agcactagac
     1561 ttgcagctgt cagataagga tttatcgaga ttggcgcgaa ttggttcggg ttctgcgtcg
     1621 cgtagtattt atggtggatt tgcagaatgg gaaaaagggt ataatgatga gacgtcatat
     1681 gccgttccac ttgaatcgaa tcattttgaa gatgaccttg ccatgatatt tgttgtgatt
     1741 aatcaacatt ctaaaaaggt acctagtcga tatggtatgt cgttgacacg aaacacatca
     1801 aggttttatc aatattggtt agatcatatt gatgaagatt tagctgaagc aaaagcagcg
     1861 attcaagaca aagattttaa acgccttggt gaagtaattg aagaaaatgg tttacgtatg
     1921 catgccacga atctgggatc aacaccgccg ttcacttatc ttgtgcaaga aagttatgat
     1981 gtcatggcgc tcgttcacga atgccgagaa gcgggatatc cgtgttattt tacgatggat
     2041 gcgggtccta atgtgaaaat acttgtagaa aagaaaaaca agcaacagat tatagataaa
     2101 ttattaacac agtttgataa taaccaaatt attgatagtg acattattgc cacaggaatt
     2161 gaaataattg agtaaggaag agataaaatg attcaggtca aagcacccgg aaaactttat
     2221 attgctggag aatatgctgt aacagaacca ggatataaat ctgtacttat tgcgttagat
     2281 cgttttgtaa ctgctactat tgaagaagca gaccaatata aaggtaccat tcattcaaaa
     2341 gcattacatc ataacccagt tacatttagt agagatgaag atagtattgt catttcagat
     2401 ccacatgcag caaaacaatt aaattatgtg gtcacagcta ttgaaatatt tgaacaatac
     2461 gcgaaaagtt gcgatatagc gatgaagcat tttcatctga ctattgatag taatttagat
     2521 gattcaaatg gtcataaata tggattaggt tcaagtgcag cagtacttgt gtcagttata
     2581 aaagtattaa atgaatttta tgatatgaag ttatctaatt tatacattta taaactagca
     2641 gtgattgcaa atatgaagtt acaaagttta agttcatgcg gagatattgc tgtgagtgta
     2701 tatagtggat ggttagcgta tagtactttt gatcatgaat gggttaagca tcaaattgaa
     2761 gatactacgg ttgaagaagt tttaatcaaa aactggcctg gattgcacat cgaaccatta
     2821 caagcacctg aaaatatgga agtacttatc ggttggactg gctcaccggc gtcatcacca
     2881 cactttgtta gcgaagtgaa acgtttgaaa tcagatcctt cattttacgg tgacttctta
     2941 gaagattcac atcgttgtgt tgaaaagctt attcatgctt ttaaaacaaa taacattaaa
     3001 ggtgtgcaaa agatggtgcg tcagaatcgt acaattattc aacgtatgga taaagaagct
     3061 acagttgata tagaaactga aaagctaaaa tatttgtgtg atattgctga aaagtatcac
     3121 ggtgcatcta aaacatcagg cgctggtggt ggagactgtg gtattacaat tatcaataaa
     3181 gatgtagata aagaaaaaat ttatgatgaa tggacaaaac atggtattaa accattaaaa
     3241 tttaatattt atcatgggca ataaatgatt tgatagggga gcaatcatct aatttaatta
     3301 gaagataaat gctccatttt ttattgccag ccgtgttacg tatcttgaaa aaaatattaa
     3361 aaagagtaaa atagatagag ttaacgaaaa aattaatgaa atcgacgata tagatatgat
     3421 aaagaaaggt gggtagcaat atgaaaaata cattccttat ttgtgatgaa tgtcaggcag
     3481 tcaatataag aacgttacaa aagaagttgg aaaaattaga tcccgatgct gaaatcgtga
     3541 taggttgtca atcttattgt ggacctggac gccgaaaaac attcactttt gttaataacc
     3601 gcccactggc tgcgcttact gaagaagaat taatcgaaaa agtttctcaa caattaaaga
     3661 aaccacgtga tcctgaagaa gaagagcgtt taagaaaacg acatgaagaa cgtaaacgtc
     3721 gtaaagaaga acaagataga aagcttaaag aaaaattaga aaagcgaaaa gcacaacaat
     3781 aaaacctgat ggcagcatca ttcaatgcgt gccaccaggt ttttatgttt tgtctagaaa
     3841 ttaaataaat cattaaatga ttcggccatc gtaggatgtg tataaatatt atctcgtaat
     3901 acggtatatg gaatgttt
//
LOCUS       DAHHSC010000211         3855 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.212, whole genome shotgun sequence.
ACCESSION   DAHHSC010000211 DAHHSC010000000
VERSION     DAHHSC010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3855)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3855)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3855
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.212"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            <1..282
                     /locus_tag="NBI57_002337"
     CDS             <1..282
                     /locus_tag="NBI57_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1823265.1"
                     /translation="IFCCLVLNKWFVSAVITFVILGVLAFFIPNFQDIKYQPLLGYAA
                     FLAIMSLLISFLLWYFTRNWRKERKARKLEKQIEKYGYEGAELRRKDKK"
     gene            230..415
                     /locus_tag="NBI57_002338"
     CDS             230..415
                     /locus_tag="NBI57_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1823266.1"
                     /translation="MKNTAMRALNFAVKIKNNIRQLYAQNHRLLSYTAVTIISKEMEY
                     SAIVVFVLTFLYFTWLT"
     gene            586..1572
                     /locus_tag="NBI57_002339"
     CDS             586..1572
                     /locus_tag="NBI57_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCD1823267.1"
                     /translation="MFIIKSMLYRLMQMIVVLFVISTLTFILMKLSPGNPVDKILHLD
                     VAQVSTEQINATKDKLGLNDSLLVQWWHWMNHLLHFNLGKSFESKEPVTQILFNYAPI
                     TLLISFSTLVVSLCISIPLGIIAAKRFHKWTDKVIRVISTLSISLPAFFIGIILLFIV
                     TNLMNIDSVILSQFILPVITLSLGMCAYIIRLVRSNLLMLLQSNIVQASRLRGMNERY
                     ILIHDLLKPTILPIIPLLGISLGSLIGGTVVIENLFDIPGIGYLLMDSIKSRDYPVIQ
                     GCVLFIGFFVVIINTIADLLTLLLDPKQRLQLGNPKNKTNTPLISESSDRHA"
     gene            1565..2395
                     /locus_tag="NBI57_002340"
     CDS             1565..2395
                     /locus_tag="NBI57_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCD1823268.1"
                     /translation="MHKIFSKNNLIFFVFVAFIFVVIVLQFFVSSENATKVNLSQTFE
                     PISWLHLLGTDDYGRDLFTRIIIGARSTLFVTVLTLIAIVVIGVTLGLFAGYKKGWIE
                     RLVLRFIDVGLSIPEFIIMIALASFFQPSLWNLVISITVIKWMNYTRLTRSIVNSEMN
                     KPYIKMAQLFHVPTRTILIRHLTPKIIPAIIVLMVVDFGKIILYISSLSFIGLGAQPP
                     TPEWGAMLQQGRDFISSHPIMLIAPASVIAITILIFNLTGDALRDRLLKQRGEYDESH
                     "
     gene            2382..3155
                     /locus_tag="NBI57_002341"
     CDS             2382..3155
                     /locus_tag="NBI57_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCD1823269.1"
                     /translation="MSLIDIQNLTIKNTCEKYLIKGIDLKIFSQQINALIGESGAGKS
                     LIAKALLEYLPFDLSCTYDSYQFDGENISRLSQYYGHTIGYISQNYAESFNDHTKLGK
                     QLTAIYRKHYKSSKEEALSKIDKALSWVNLQSKDILNKYSFQLSGGQLERVYIASVLM
                     LEPKLIIADEPVASLDALNGNQVMDLLQHIVLEHGQTLFIITHNLSHVLKYCQYIYVL
                     KEGQIIERGNINHFKYEHLHPYTERLIKYRTQLKRDYYD"
     gene            3148..3849
                     /locus_tag="NBI57_002342"
     CDS             3148..3849
                     /locus_tag="NBI57_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCD1823270.1"
                     /translation="MIELKHVTFGYNKKQMVLQDINITIPDGENVGILGESGCGKSTL
                     ASLVLGLFKPVKGEIYLSDNAVLTIFQHPLTSFNPDWTIETSLKEALYYYRGLTDNTA
                     QDQLLLQHLSTFELNAQLLTKLPSEVSGGQLQRFNVMRSLLAQPRVLICDEITSNLDV
                     IAEQNVINILKAQTITNLNHFIVISHDLSVLQRLVNRIIVLKDGMIVDDFAIEELFNV
                     DRHPYTKELVQTFSY"
BASE COUNT         1288 a          533 c          668 g         1366 t
ORIGIN      
        1 atattctgtt gcttagtttt aaataaatgg tttgtatctg cagtaattac atttgttata
       61 ttaggtgtgc ttgcattttt tattccaaat tttcaagata taaaatatca accattatta
      121 ggatacgctg catttttagc gattatgagt ttattaatta gttttctatt atggtatttt
      181 actagaaatt ggcgtaaaga aagaaaagcg cgtaaattag aaaagcaaat tgaaaaatac
      241 ggctatgagg gcgctgaact tcgccgtaaa gataaaaaat aatattcgac aactatatgc
      301 acaaaatcat cgacttttaa gttacactgc agtaacaatt atttcgaaag agatggaata
      361 cagtgctata gttgtttttg ttttgacttt tttatatttt acatggttga cttgattcaa
      421 aatattatat tttataatga ttttgttaaa aaattaaata tacttcaatt tctttatgga
      481 taatactata atgaacgttg gttcttaata tgtaagttgc atatacatgc gccattctca
      541 tgtaaaaaga aagtgttagt aacttacaca aggaggggga ctattatgtt cataataaag
      601 tctatgttgt atagactgat gcaaatgata gttgttttat ttgttatttc tacacttaca
      661 ttcattttaa tgaaattatc accaggcaat cctgttgata aaatattaca tcttgatgta
      721 gcacaagtgt cgacggaaca aataaatgca accaaagata aattgggatt aaatgattct
      781 ttattggtac agtggtggca ttggatgaat catttattac attttaattt aggaaaaagt
      841 tttgaatcaa aagaaccggt aacacagata ttattcaact atgcacccat aacattactc
      901 atatcatttt caacattagt agtgtcgtta tgcatatcta taccgctcgg cattatagca
      961 gccaaacgat ttcataagtg gacggataaa gtgattagag tgatctcaac actatcgatt
     1021 agtttaccag cgttctttat cggtattatt ttattattta ttgtcacaaa tttaatgaat
     1081 atagatagcg ttatactaag tcagtttata ttacctgtaa tcacgctatc tttaggtatg
     1141 tgtgcataca tcattcgttt agtgcgttct aatttattga tgttattgca aagtaatatc
     1201 gtacaagcat caagattacg cggtatgaat gaacgttata ttttaattca tgatttacta
     1261 aaaccaacaa ttttgccgat tatcccatta ctagggattt cacttggcag tctaataggt
     1321 ggtactgtag tgattgaaaa tttatttgat atacctggta ttggttatct attaatggat
     1381 agtattaaat ctcgagatta tcctgttatt caaggatgcg tgttatttat tggcttcttc
     1441 gttgttatta tcaatacgat tgctgattta ttaacgttat tacttgatcc gaagcagcgt
     1501 ttacaattag gaaatccaaa aaacaaaacc aatacaccat tgatatcaga aagtagtgac
     1561 cgtcatgcat aaaatatttt caaagaataa cctgatattt tttgtattcg ttgcatttat
     1621 ttttgtggta attgtactgc aattctttgt cagtagtgaa aatgcaacca aagtcaattt
     1681 atcacaaact tttgaaccta ttagttggtt gcatttatta ggaactgatg attatgggag
     1741 agatttattt acccgaatta ttatcggtgc acgttcaaca ttgtttgtta ctgttttaac
     1801 attaatagct atcgttgtca taggtgttac actaggtcta tttgccggat ataaaaaagg
     1861 gtggattgaa cgattagtgt taaggtttat tgatgttggt ctaagtattc cagaatttat
     1921 catcatgatt gctttagcaa gcttttttca accatctttg tggaatttag ttatctcaat
     1981 tacagtaata aaatggatga attacacaag gttgactaga agtatagtta atagcgaaat
     2041 gaataagcct tatataaaaa tggcacaatt atttcatgta cctacaagaa caatattaat
     2101 acgtcattta acacctaaaa ttataccggc tattatcgtt ttgatggtcg ttgatttcgg
     2161 taaaatcatt ttatatataa gttcactatc atttattggg ttaggtgcac aaccgccaac
     2221 accagagtgg ggcgctatgt tgcaacaagg tcgtgatttt atttcgtctc atccaattat
     2281 gttgattgca cctgcttcag tcattgctat aactatttta atttttaatt taaccggaga
     2341 tgcgctaaga gatagattgc taaaacaacg aggtgaatat gatgagtctc attgatatac
     2401 aaaatttaac aataaagaat acttgtgaga aatatcttat taaagggatt gatttgaaaa
     2461 tttttagtca acagattaat gccttgattg gagagagcgg cgctgggaaa agtttgattg
     2521 ctaaagcctt acttgaatat ttaccatttg atttaagctg cacgtatgat tcgtaccaat
     2581 ttgatggaga aaatattagt agattgagtc aatattatgg tcatacaatt ggttatattt
     2641 ctcaaaatta tgcagaaagt tttaacgacc atactaaatt aggtaaacag ttaactgcga
     2701 tttatcgtaa gcattataaa agtagtaaag aagaggcttt gtcaaaaatt gacaaggctt
     2761 tgtcgtgggt taatttacaa agcaaagata tattaaataa atatagtttc caactttctg
     2821 ggggccaact tgaacgcgtt tacatagcaa gcgttctcat gttggagcct aaattaatca
     2881 ttgcagacga accagttgca tcattggatg ctttgaacgg taatcaagtg atggatttat
     2941 tacagcatat tgtattagaa catggtcaaa cattatttat tatcacacat aacttaagtc
     3001 atgtattgaa atattgtcag tacatttatg ttttaaaaga aggtcaaatc attgaacgag
     3061 gtaatattaa tcatttcaag tatgagcatt tgcatccgta tactgaacgt ctaattaaat
     3121 atagaacaca attaaagagg gattactatg attgagttaa aacatgtgac ttttggttat
     3181 aataaaaagc agatggtgct acaagatatc aatattacta tacctgatgg agaaaatgtt
     3241 ggtattttag gcgaaagtgg ctgtggtaaa agtacgctcg cttcattggt tcttggcttg
     3301 tttaaacctg ttaaaggaga aatttactta agtgataatg ctgtgttaac gattttccaa
     3361 caccctttaa ctagctttaa ccctgattgg acgattgaga cctcattaaa agaagcgtta
     3421 tattattaca gaggcctaac tgataatact gctcaggatc aattattatt acaacattta
     3481 tctacttttg agttaaacgc gcaattattg actaaattac caagcgaagt gagtggcgga
     3541 caattacaaa gatttaatgt catgcgttcg ttattagcac agcctcgcgt tttaatatgt
     3601 gatgagataa cttcaaattt agatgttata gctgaacaaa atgtaatcaa tatattaaaa
     3661 gcgcaaacga ttacgaactt aaatcatttt atcgttattt ctcatgattt atccgtgtta
     3721 caacgcttag ttaatagaat tatcgttctt aaggatggca tgatagtcga tgattttgca
     3781 atagaggaat tatttaatgt tgatagacac ccttatacaa aagaattagt gcaaacattt
     3841 tcatattagc tattt
//
LOCUS       DAHHSC010000212         3773 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.213, whole genome shotgun sequence.
ACCESSION   DAHHSC010000212 DAHHSC010000000
VERSION     DAHHSC010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3773)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3773)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3773
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.213"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            363..3437
                     /gene="mupA"
                     /locus_tag="NBI57_002343"
     CDS             363..3437
                     /gene="mupA"
                     /locus_tag="NBI57_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mupirocin-resistant isoleucine--tRNA ligase
                     MupA"
                     /protein_id="HCD1823271.1"
                     /translation="MTKKYLNTQNEISAFWNTQKIFKKSIDNRKGQESFVFYDGPPTA
                     NGLPHAGHVLGRVIKDLVARLKTMQGFYVERKAGWDTHGLPVELEVEKKIGIKGKQDI
                     EKYGIENFINECKKSVFNYEKEWRDFSKDLGYWVDMDSPYITLENNYIESVWNILSTF
                     HKKGLLYKGHKVTPYCTHDQTALSSHEVAQGYKNVKDLSAVVKFQLTNSKDTYFLSWT
                     TTPWTLPANVALAINKDLNYSKIRVENEYYILATDLINSIITEKYEIIDTFSGSNLIN
                     LKYIPPFESDGLVNAYYVVDGEFVTNSEGTGIVHIAPAHGEDDYQLVLERDLDFLNVI
                     TREGVYNDRFPELVGNKAKNSDIEIIKLLSKKQLLYKKQKYEHNYPHCWRCGNPLIYY
                     AMEGWFIKTTNFKNEIINNNNNIEWFPSHIKEGRMGNFLENMVDWNIGRNRYWGTPLN
                     VWICNDCNHEYAPSSIKDLQNNSINKIDEDIELHRPYVDNITLSCPKCNGKMSRVEEV
                     IDVWFDSGSMPFAQHHYPFDNQKIFNQHFPADFIAEGVDQTRGWFYSLLVISTILKGK
                     SSYKRALSLGHILDSNGKKMSKSKGNVINPTELINKYGADSLRWALISDSAPWNNKRF
                     SENIVAQTKSKFIDTLDNIYKFYNMYNKIDHYNPNNEITKSRNTLDNWALSRLNTLIK
                     ESNIYVNNYDFTSAARLINEYTNTISNWYIRRSRGRFWEQGISNDKKDAYNTLYEILT
                     TLSRLVAPFVPFISEKIHYNLTGKSVHLQDYPQYKESFINQALEDEMHTVIKIVELSR
                     QARKNADLKIKQPLSKMVIKPNSQLNLSFLPNYYSIIKDELNIKNIELTDNINDYITY
                     ELKLNFSSVGPKLGNKTKNIQTLIDSLSEYDKKSLIESNNFKSLSSDAELTKDDFIIK
                     TLPKDSYQLSEDNDCVILLDKNLSPELIREGHARELIRLIQQLRKKKNLPINQRIDIY
                     IGVTGELLESIKTNKNMFKENLLIKNIHLNVIDEYENTIHFNNKEIKISLLY"
     gene            3507..>3773
                     /locus_tag="NBI57_002344"
     CDS             3507..>3773
                     /locus_tag="NBI57_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000282188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HCD1823272.1"
                     /translation="MYNFDYSKLPIKNIQKIFPIAGGYVNLSFSVDASNKKYFLKLQP
                     NTKSNFFDYELSSLKELTDKNIPVPQIINKGELDNNSFLLLEFIE"
BASE COUNT         1490 a          473 c          587 g         1223 t
ORIGIN      
        1 ggtattttaa ttatagattt gatgaacccc gcctttttct ataaaaattt tgaagaacaa
       61 atgattttca acttcaaaga ttacgaagtt aatcaaacta ttaaaataaa taaaatttat
      121 aatagtatag tttcaacatg ggatgtttat aaaaataacg aatatagcaa agacctttca
      181 agagaattaa aatttaaatt atatactact aatgatataa tttgcagtag tttgtattat
      241 aactttactt tatccaatat atctttctat ggagacatga aaggaaatga atttcatgaa
      301 aattcaccaa gactaatagt aaaaataacg aaataagtga tactctagga ggctgaaaag
      361 ttttgacaaa gaaatattta aacacccaga atgaaatatc agcattttgg aatactcaaa
      421 agatatttaa aaaatcaatt gacaatagaa aaggacagga aagttttgtt ttttatgacg
      481 gccccccaac tgcaaatggc cttcctcatg ctggccatgt tcttggaaga gtaatcaagg
      541 atttagttgc aagattaaaa actatgcaag gtttttatgt agaaagaaaa gcaggatggg
      601 atacccatgg cttaccagtt gaattagagg ttgaaaaaaa aattggaatt aaaggaaaac
      661 aagacattga aaagtatgga atagaaaatt ttataaatga atgtaaaaaa agtgtattta
      721 attatgaaaa agaatggcgg gatttttcta aagatttagg atactgggtt gacatggact
      781 ccccctatat aactcttgag aataattata ttgaaagtgt atggaatata ttatctacat
      841 tccataaaaa aggactatta tataagggac ataaggtgac tccttattgt acacatgatc
      901 aaaccgcttt aagttctcat gaagtagcgc aaggctataa aaacgttaaa gatttatcag
      961 ctgttgttaa atttcaactt acaaatagta aagatactta tttcttaagt tggactacca
     1021 ctccctggac tttgcctgca aatgtagcat tagctataaa taaagatctt aattattcaa
     1081 aaattcgggt agaaaatgag tattatatct tagctacaga tctaattaat tctataataa
     1141 ctgaaaaata cgaaattatt gatacctttt caggaagtaa tttaattaat ttaaaataca
     1201 ttcctccttt tgaaagcgac ggtttagtta atgcatatta cgttgttgat ggagaatttg
     1261 ttactaactc agaaggaact ggtattgttc atatagcacc agctcatggg gaagatgact
     1321 accaattggt tttagagcgt gatttggatt tcttaaatgt tataacaaga gaaggagtat
     1381 ataatgatag gttccctgaa ttagttggta ataaagctaa aaatagtgat atagaaatca
     1441 taaaattatt atccaaaaaa caacttttat ataaaaaaca aaaatatgag cataattatc
     1501 ctcattgttg gagatgtggt aatcctttga tatattatgc gatggagggt tggtttatta
     1561 aaacaactaa ttttaagaat gaaattatta acaataataa taatatagag tggtttcctt
     1621 ctcatattaa ggaagggaga atgggaaatt tcttagaaaa tatggttgat tggaacattg
     1681 gtagaaatag atattgggga acaccattaa atgtatggat ttgcaatgat tgtaatcacg
     1741 aatacgcacc aagtagtatt aaggatttac aaaataattc catcaataaa attgatgaag
     1801 atattgagtt gcatagacct tatgttgata atatcactct tagttgccct aagtgtaatg
     1861 ggaaaatgtc tcgagtagaa gaagtaatcg atgtttggtt tgatagcggc tctatgccgt
     1921 ttgctcagca tcattatcct tttgataacc agaaaatttt taatcaacac tttccagctg
     1981 attttattgc agaaggagtt gatcaaacga gaggctggtt ttacagttta ctagtaattt
     2041 ctactattct aaaaggaaaa tcttcttata aacgtgcttt atctttagga catattctag
     2101 acagtaatgg taaaaaaatg tctaaaagta aaggaaacgt tattaatcca actgaattaa
     2161 ttaataagta cggagccgat tctttaagat gggccttaat ttcggatagt gctccatgga
     2221 ataacaaaag attctcagaa aatatagtag ctcagaccaa atcgaaattt atagatacgc
     2281 ttgataatat ttataaattt tataatatgt ataataaaat agatcactat aatcctaata
     2341 atgaaattac aaaaagtaga aatacattag ataattgggc tctttctcgc ttaaacacct
     2401 taataaaaga aagtaatatt tatgtaaata attacgattt cacttccgca gccagattaa
     2461 ttaacgaata taccaataca ataagtaatt ggtatattcg gagatcgaga ggacgatttt
     2521 gggaacaagg aatttctaac gataaaaaag atgcgtacaa tacgctttat gaaattttaa
     2581 caactttatc aagactagtg gctccatttg ttccatttat atctgaaaaa atccattata
     2641 atttgactgg aaaaagtgtg catttacaag attatccaca atataaagaa agttttatta
     2701 atcaagcatt ggaagatgaa atgcataccg ttataaaaat tgtagaatta tctagacagg
     2761 ctcgcaaaaa tgcagattta aaaattaagc aacctttatc gaaaatggtg attaaaccta
     2821 atagtcaatt aaacttaagt tttttaccta attactattc aataataaaa gacgaattaa
     2881 atataaaaaa cattgaatta actgataata ttaatgacta tattacctat gagcttaaat
     2941 tgaatttttc ttctgtggga ccaaaactag ggaacaaaac gaaaaatatt caaacattga
     3001 tagactccct atcagagtat gataaaaaaa gtttaattga gtctaataac ttcaaaagtt
     3061 tatcttctga tgctgagtta actaaggatg attttataat taaaacctta cctaaggata
     3121 gttatcaact cagtgaagat aatgactgcg ttatattatt agataaaaat ttatctcctg
     3181 aattaattcg cgaaggacat gctagagagc tcattagatt aattcaacaa ttaagaaaaa
     3241 agaaaaattt accaataaat caacgtattg atatttatat cggtgtaact ggggaattat
     3301 tagaatcaat aaaaaccaat aaaaatatgt ttaaagaaaa tttgctgatt aaaaatatac
     3361 acttaaatgt tatagatgaa tatgaaaata ctattcattt taataataaa gaaataaaaa
     3421 tttccttatt atattaaaca aacatggcca ctctatttta gtagagtggc catgagttat
     3481 caaagccaag caaaaggtga taaactatgt ataattttga ttattctaaa ttaccaatta
     3541 aaaatattca aaaaatattt cctattgcag ggggatatgt gaatctatct ttttcagttg
     3601 atgcttccaa caaaaaatat tttttaaagc tacaacctaa tactaaatca aatttttttg
     3661 attatgagtt atccagttta aaagaattaa ctgataaaaa tattcctgtt cctcaaatta
     3721 ttaataaagg agaattagac aataattctt ttttactgtt agagttcatt gaa
//
LOCUS       DAHHSC010000213         3760 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.215, whole genome shotgun sequence.
ACCESSION   DAHHSC010000213 DAHHSC010000000
VERSION     DAHHSC010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3760)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3760)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3760
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.215"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            complement(9..242)
                     /gene="nrdH"
                     /locus_tag="NBI57_002345"
     CDS             complement(9..242)
                     /gene="nrdH"
                     /locus_tag="NBI57_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HCD1823273.1"
                     /translation="MSEIIVYTQNDCPPCTFVKNYLNEHQIDFEERNINNQQYRNEMI
                     DFDAFSTPFILLNGNPMYHVDLDEINKVLNIQD"
     gene            440..1801
                     /locus_tag="NBI57_002346"
     CDS             440..1801
                     /locus_tag="NBI57_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HCD1823274.1"
                     /translation="MDTVEISRFLTAMTLAVHIIFATIGVGMPLMFAIAEFLGIRKND
                     LQYIAMAKRWAKAYTITVAVGVVTGTIIGLQLSLIWPTFMEMGGHVIALPLFMETFAF
                     FFEAIFLSIYLYTWDRFKNKWTHFLISIPVIIGGSFSAFFITSVNSFMNTPAGFELKN
                     GKMVNVQPIEAMFNPSFIVRSFHVITTAGMTMAFVIASIAAFKLLRNRQPKDTVYHKK
                     ALKMSMIVGFFSTLLSMLAGDLSAKFLHKFQPEKLAAYEWHFDTSSHAKLLLFGVLDE
                     KTQQVKGAIELPGLLSFLADNSVKTKVQGLNDFPKSLHPPMIVHYFFDLMVTMGILCF
                     VISGVYVLTLMFKKLRKFSTHKWMLYGILLTGPASMLAIEFGWFLTEMGRQPWIVRGY
                     MRVAEAATQAGGITFVTILFGILYIILMYTCAYVLIRMFKNKPAYEDVNRLAKKQGGE
                     IEK"
     gene            1798..2817
                     /locus_tag="NBI57_002347"
     CDS             1798..2817
                     /locus_tag="NBI57_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HCD1823275.1"
                     /translation="MIYAFIGITVLWLFLFCYIIIASIDFGAGFFALHSKLTGDEKKI
                     NHLISRYLNPVWEVTNVFFVFFFVGFVGFFPESIKYLGTVLLIPGSIALIMISLRNSF
                     YAFENYGQDTKLAWMIMYGVSGLLIPASLSTALTITEGGYINVRNNVIDLDWVQLLLS
                     PFAWSVVFLAIISVLYISSGFLTYYAKKANDEPAYNLTRQWHIFLGPPMIIICLFVFL
                     SLRIQNSEHFYSAVFDYWWMFAISFLFFALASLLTFFKKKHGLAFVFVILQMMFAFFG
                     YGISKLPYLLYPFVKITDAYVNPEMGWTLVIVFILGLLLLLPSLILLLRLFVFDKEYV
                     EGKKS"
     gene            2950..3612
                     /locus_tag="NBI57_002348"
     CDS             2950..3612
                     /locus_tag="NBI57_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HCD1823276.1"
                     /translation="MGKEYVVIGLGRFGGSIVRELNALDMDVMAIDHDENRVNEYSDI
                     ATHAVVADTTDEAVMKSLGIRNFDHVIVAIGENIQSSTLTTLILKELGVKKVTAKAQN
                     DYHAKILNKIGADTVVHPERDMGRRIAHNVASASVLDYLELADEHSIVELKATEKMAG
                     QSIIDLDIRAQYGINIIAIKRGKEFIISPNPNINLEIGDILIMIGHDNDLNRFEKNIA
                     TR"
BASE COUNT         1189 a          522 c          698 g         1351 t
ORIGIN      
        1 gatgaaaatt aatcttggat atttaatact ttgttgattt catcaagatc aacatggtac
       61 attggattgc cattcaacaa aataaacgga gttgaaaaag catcaaaatc tatcatttcg
      121 tttcgatatt gttgattgtt gatatttctc tcttcaaaat caatttgatg ctcatttaga
      181 taatttttta caaatgtaca aggtggacaa tcattctgcg tataaacgat tatttctgac
      241 atgtatcatg ctccttcgtg ctttattcta atgtaatata cacaattata cattgaaatt
      301 caaatttgtg aacacattgt gaactgacat aaattataca caatttcata gcgcataatg
      361 ttttaaaaat acacattata gcaattataa ttaagttcat tgtgttgtca tcaaaaaaag
      421 aaaaaggtga tgtttttaaa tggatacagt tgaaatcagt cggtttttga cagctatgac
      481 tttagcagtt catatcattt ttgcaacgat tggtgttggt atgcctttaa tgttcgcaat
      541 tgcagaattt ttaggtattc gcaaaaatga tcttcaatat atagctatgg ccaaaagatg
      601 ggctaaagct tatacaatta ctgtagcagt gggagttgtt acaggtacaa ttataggact
      661 tcaattatca ttgatttggc ctacatttat ggaaatgggt ggacacgtta ttgcacttcc
      721 attatttatg gaaacatttg cgttcttctt tgaagctatt ttcttaagta tatatttata
      781 tacttgggat cgttttaaaa ataaatggac acatttctta attagtatac cagtaattat
      841 tggtggctct ttctcagcct tcttcattac ttcagtgaat tcatttatga atacgcctgc
      901 aggttttgag ttgaagaatg gaaagatggt caatgttcaa cctatagaag cgatgtttaa
      961 cccatcgttt atagttcgat catttcacgt aattactaca gccggtatga cgatggcatt
     1021 tgttattgca tcaatagcag cttttaaatt attgcgtaat cgtcaaccta aagatactgt
     1081 ctaccataag aaagctttga aaatgtctat gattgttgga ttcttttcaa ctttactttc
     1141 tatgttggca ggggatttat ctgcaaaatt tttgcataaa ttccaacctg aaaaattagc
     1201 agcttatgaa tggcatttcg atacatcttc ccatgctaaa ttattattat ttggtgtgtt
     1261 agatgaaaag actcagcaag ttaaaggtgc gattgaatta cctggactat tgagtttctt
     1321 agcagataat agtgtcaaaa ctaaagtgca agggttaaat gattttccaa aaagtttaca
     1381 tccgcctatg attgtccatt atttctttga tttaatggta acgatgggaa ttttatgttt
     1441 tgtcatttca ggtgtttatg ttttaacttt aatgtttaaa aagcttagaa agttttctac
     1501 tcataaatgg atgctttacg gaatattatt aacaggacca gcttcaatgc tagctataga
     1561 atttggatgg ttcttaacag agatgggtag acagccttgg attgttcgtg gttatatgcg
     1621 cgtggcagaa gcagcaacac aagcaggcgg aataaccttc gttacaattt tatttggcat
     1681 attgtacatc attttaatgt atacatgtgc atacgtatta attcgtatgt ttaaaaataa
     1741 accggcgtat gaagatgtaa atcgtttagc caagaagcaa ggaggagaaa tagaaaaatg
     1801 atttatgcat ttataggtat aacagtgtta tggttgtttt tattttgcta tatcattatt
     1861 gcttctatag attttggggc aggtttcttc gcattgcatt caaagttaac tggtgatgaa
     1921 aagaaaatta atcacttaat ttcacgttat ttgaacccag tttgggaagt tacgaatgta
     1981 ttctttgttt tcttcttcgt aggattcgta ggtttctttc cagagtcaat caaatattta
     2041 ggcacggtat tgttaatacc aggttcaata gcactgatta tgatatcatt gagaaacagt
     2101 ttttatgcat ttgaaaatta tggtcaagat acaaaattag catggatgat catgtatggg
     2161 gtaagtggat tattaattcc agcttcatta tctactgctt taactattac agaaggtggc
     2221 tatattaatg ttcgaaacaa tgttatcgat ctagattggg tgcagttact attaagccca
     2281 tttgcttggt ctgtagtatt cttggcaatt atttcagttt tatatatttc atcaggattt
     2341 ttgacatatt atgctaaaaa agcaaatgac gaaccagcat ataatttaac aagacaatgg
     2401 cacatatttt tagggccacc gatgattatc atttgtttat tcgtatttct atcattacgc
     2461 attcaaaatt ctgaacattt ttattcagct gtttttgact actggtggat gtttgctata
     2521 agtttcttat tctttgcatt agcttcattg ttaacattct ttaagaaaaa acatggtttg
     2581 gcttttgtat ttgttatttt acaaatgatg ttcgcgttct ttggctatgg aattagtaaa
     2641 ttgccatacc ttttatatcc gtttgtaaaa attacagatg catatgttaa tccagaaatg
     2701 ggctggacat tagtgattgt ctttatttta ggtttacttt tattacttcc atcgttaata
     2761 ttattattaa gattatttgt tttcgacaaa gaatatgttg aaggaaagaa atcataagtt
     2821 gagacgatac cccaactttc atttacttgc attactgcat ttagataatt tagttggggt
     2881 actcatttat aataatttta aatttatatt ttcaatgaat tgatgaaaat catttaatgg
     2941 agggcgagta tgggtaaaga atatgtagtc atcggtctag gtcgttttgg aggtagtatc
     3001 gttcgtgagt tgaatgcatt agacatggat gtaatggcca tcgaccatga tgaaaataga
     3061 gtgaatgaat atagtgatat cgctactcat gcggttgttg cagacacaac agatgaagca
     3121 gttatgaaaa gtttaggtat ccgtaacttt gatcatgtca ttgtggcaat tggtgaaaat
     3181 attcaatcaa gtacgttgac gactttaatt ttaaaagagt taggtgtaaa aaaagtaact
     3241 gctaaagcac aaaatgatta tcatgcaaag attttaaata aaattggagc agatacggtt
     3301 gtgcaccctg agcgtgatat gggtagacgt attgcgcata atgttgcgag tgcaagtgta
     3361 cttgattatc ttgagttggc agacgagcat tctattgtag aattgaaagc aactgaaaag
     3421 atggcggggc agtctatcat tgatttagat ataagagcac aatatggaat taacattatt
     3481 gcaattaaac gaggcaaaga gttcatcatt tcaccaaatc caaatattaa tttagaaata
     3541 ggtgatattt taatcatgat tggacatgat aatgatttaa atcgctttga aaaaaatatt
     3601 gcgacgagat aatcgggagt gggacagaaa tgatattttc tcaaaattta tttcgtcgtc
     3661 ccaccccggc aaggttgact agaattgaaa aaagcttgtt acaagcgcat tttcgttcag
     3721 tcaactactg ccaatataac tttgtagagc attgaacatt
//
LOCUS       DAHHSC010000214         3684 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.216, whole genome shotgun sequence.
ACCESSION   DAHHSC010000214 DAHHSC010000000
VERSION     DAHHSC010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3684)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3684)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3684
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.216"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            complement(1..142)
                     /locus_tag="NBI57_002349"
                     /pseudo
     CDS             complement(1..142)
                     /locus_tag="NBI57_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500888.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(231..1295)
                     /locus_tag="NBI57_002350"
     CDS             complement(231..1295)
                     /locus_tag="NBI57_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017804887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-loop NTPase"
                     /protein_id="HCD1823277.1"
                     /translation="MLTVDQVKELVGEIKDPIIDVPLKETEGIVEVSIKEEKEHVSVK
                     LAMAQLGGAPQLDLQMAVVNALKENGAKTVGIRFETLPEEKVNQFKPKEENKPKTIEG
                     LLSQNNPVEFIAIASGKGGVGKSTVAVNLAVALAREGKKVGLVDADIYGFSVPDMMGI
                     DEKPGIKGKEVIPVERHGVKVISMAFFVEENAPVIWRGPMLGKMLTNFFTEVKWGDIE
                     YLILDLPPGTGDVALDVHTMLPSSKEIIVTTPHPTAAFVAARAGAMAKHTDHSILGVI
                     ENMSYFESKETGNKEYVFGKGGGTKLADELNTQLLGELPLEQPSWNPKDFAPSIYQSD
                     DRLGKIYSSIAQKVIAATNK"
     gene            complement(1443..2885)
                     /gene="lmrS"
                     /locus_tag="NBI57_002351"
     CDS             complement(1443..2885)
                     /gene="lmrS"
                     /locus_tag="NBI57_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter LmrS"
                     /protein_id="HCD1823278.1"
                     /translation="MAKVELTTRRRNFIVAVMLISAFVAILNQTLLNTALPSIMRELN
                     INESTSQWLVTGFMLVNGVMIPLTAYLMDRIKTRPLYLAAMGTFLLGSIVAALAPNFG
                     VLMLARVIQAMGAGVLMPLMQFTLFTLFSKEHRGFAMGLAGLVIQFAPAIGPTVTGLI
                     IDQASWRVPFIIIVGIALVAFVFGLVSISSYNEVKYTKLDKRSVMYSTIGFGLMLYAF
                     SSAGDLGFTSPIVIGALIISMVIIYLFIRRQFNITNVLLNLRVFKNRTFALCTISSMI
                     IMMSMVGPALLIPLYVQNSLSLSALLSGLVIMPGAIINGIMSVFTGKFYDKYGPRPLI
                     YTGFTILTITTIMLCFLHTDTSYTYLIVVYAIRMFSVSLLMMPINTTGINSLRNEEIS
                     HGTAIMNFGRVMAGSLGTALMVTLMSFGAKIFLSTSPSHLTATEIKQQSIAIGVDISF
                     AFVAVLVMAAYVIALFIREPKEIESNRRKF"
     gene            complement(3208..3681)
                     /gene="sepA"
                     /locus_tag="NBI57_002352"
     CDS             complement(3208..3681)
                     /gene="sepA"
                     /locus_tag="NBI57_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter SepA"
                     /protein_id="HCD1823279.1"
                     /translation="MIVNYLKHKFYNLLTTMIVLFIFVLSGAIFLTFLGFGLYGLSRI
                     LIYFRLGDFTYNRSMYDNLLYYGSYIIFGYFIIFAVEHLMDYFRKMLPENAYFRGATF
                     HLISYTVATTLFYFIIHLNYVYINIDFWVIMVIIGFLYVCKLQFYPESKNLNNRK"
BASE COUNT         1287 a          715 c          476 g         1206 t
ORIGIN      
        1 ttgaaattct tctttaaaaa caactgcgtc gtttttgacg actttatcat attatcaact
       61 ttggaattta aagtcaataa cttttttaaa actttttgtg ttcacaaccc gcttcttttt
      121 caacgcgttt attgcttaac acaagaactt attttaccag cattccaaaa caaatcaaca
      181 taaaaacgta caaaataaaa gtaattttgt acgtttagca tatattatac ctatttattt
      241 gtagcagcta taactttttg tgcaatcgag ctataaattt tacctagacg atcatctgat
      301 tgatatattg acggtgcaaa atcttttgga ttccaagatg gttgctctaa aggtaattcc
      361 ccaagtaatt gagtattaag ttcatcagct aacttagtac cgccaccttt gccaaagaca
      421 tattctttat tacccgtctc tttactttca aaataactca tgttttcaat tacgccaaga
      481 atagaatgat ccgtatgttt tgccatcgca cctgcgcgag ctgcaacaaa tgctgctgta
      541 ggatgaggtg tcgttacaat aatttcctta cttgaaggta acatcgtatg aacatctaaa
      601 gctacatctc ctgttccagg tggaagatcg agtattaaat attcaatgtc tccccattta
      661 acttctgtaa agaaattcgt caacatttta cctaacattg gccctctcca tataactggc
      721 gcattttctt ccacaaaaaa ggccattgat ataactttaa cgccatgacg ttcaactgga
      781 attacttcct tccctttaat tccaggcttt tcatcaatac ccatcatatc tggtacacta
      841 aatccatata tatcggcatc tactaatccg acttttttcc cttcacgagc taaggcaacg
      901 gctaaattta ctgcaacagt agatttaccg acaccacctt taccggaggc tattgcaata
      961 aattcgactg gattattttg agataatagg ccttctatcg ttttaggttt attttcttct
     1021 tttggtttaa attgatttac tttttcttcc ggcaatgttt caaatcgtat accgaccgtt
     1081 ttcgcaccgt tttcttttaa tgcattaaca acagccatct gtaaatctaa ttgcggtgca
     1141 ccacctaatt gtgccattgc taatttaaca ctcacatgtt ctttttcttc cttaatagaa
     1201 acttcaacaa taccttctgt ttcttttaaa ggcacatcta taataggatc tttaatttct
     1261 cctaccaatt ctttcacttg atctaccgtt aacaaagcaa atccccctta atcattttaa
     1321 gacatctaca tcatagcaaa taacaatttt tgcttagtct atcactaaaa gtataaaact
     1381 ttttattaaa taaaaaaagc agttagccta ttattttaaa tagaccaact actataatta
     1441 ttttaaaatt tccttctatt actttctatt tctttaggtt ctcttataaa aagtgctatc
     1501 acataagctg ccataacaag cacagctaca aaagcaaatg agatatccac ccctatagca
     1561 atggattgct gtttaatttc agttgcagtt aaatgcgatg gcgatgtaga taaaaatatt
     1621 tttgcaccaa aactcattaa tgtaaccatt aaagctgtgc ctagtgaacc agccattaca
     1681 cgaccaaagt tcataatagc cgtgccatgt gagatttctt catttctcaa agaattaatt
     1741 cctgtagtat ttatcggcat catgagtaaa gaaactgaaa acattctaat tgcatatacg
     1801 actattaaat acgtataaga tgtgtctgta tgcaagaaac acaacataat tgtagtaatt
     1861 gttaaaattg taaaaccagt ataaatcaat ggtctaggac catacttatc ataaaattta
     1921 cctgtaaaaa ctgacataat accatttatt attgcaccag gcatgataac aagtcctgat
     1981 aacaaggcag ataaagataa actattttga acatatagcg gtataagcag cgcaggtcca
     2041 accattgaca tcattataat cattgaacta atcgtacata atgcaaatgt tctattttta
     2101 aaaaccctta aatttaaaag tacattagta atattaaatt gtctacgtat aaataaatag
     2161 ataataacca tacttattat caacgcacct attactattg gacttgtaaa tcctaaatct
     2221 cctgcactac taaatgcgta tagcattaac ccgaatccaa tagttgaata cattactgag
     2281 cgcttatcta atttcgtata tttcacttca ttgtaacttg agattgaaac taagccgaaa
     2341 acaaaggcaa ctaaagcaat tcctacaatt ataataaatg gtactcgcca actcgcttga
     2401 tcaataatta atcctgtaac tgtagggcct attgctggtg caaattgaat tactaaacct
     2461 gctagtccca ttgcaaaacc tcgatgttct ttactgaaca atgtaaataa cgtaaattgc
     2521 attaagggca taagtacgcc tgcacccatc gcttgaatta cacgtgctaa cattaaaact
     2581 ccaaaattcg gagctaaggc tgcaacaata gaacctaata aaaatgtccc catcgccgct
     2641 aagtataaag gtctagtttt aattctatcc attagatatg ccgtcagagg tatcatgacg
     2701 ccattaacaa gcataaaccc agtaactagc cattgcgatg tactttcatt aatatttaat
     2761 tctctcatta tactaggtaa cgctgtattt aataatgttt gatttaaaat agctacaaac
     2821 gcactaatca acataacagc aactatgaag tttcgtctcc gggttgttaa ttcaacttta
     2881 gccatcataa ttcctccttt ttaaaacaga tatacacttg tccctttaat acatatagcc
     2941 attaacataa tgggagtagt actcaaatga taaagaagca ctacataata aatcattatt
     3001 ggttcttaca cattaggcac agctaatgtg tacttaaaaa actaacaata taacattgta
     3061 gggcctaata atttatttta tttctcagac tcatacaatg gttatttaac ttatttacca
     3121 caaattgagc actaagactc actattatcg attcttctat tgaacaaaaa gaagttggaa
     3181 cacatatgtg ccctcaactt cagatatcta ttttctatta tttaaatttt tactttcagg
     3241 gtaaaattga agcttacaaa catataagaa ccctataata accatgatga cccaaaaatc
     3301 gatattgata taaacataat ttaaatgaat gatgaaataa aataacgttg ttgcaactgt
     3361 gtaagaaatc aaatgaaaag tcgcgcctct aaaatatgca ttttcaggca acatttttct
     3421 aaaataatcc attaaatgct cgactgcaaa tatgatgaag tacccaaaga ttatataact
     3481 gccataatat aataggttat cgtacatact tctattatat gtaaagtcac ccaacctaaa
     3541 ataaataagt attctactta atccatataa accaaaccct agaaatgtta aaaaaatcgc
     3601 accagaaagt acaaaaataa agagaacaat cattgtagtt aacaagttat aaaacttatg
     3661 tttaagataa ttcacaatca ttgg
//
LOCUS       DAHHSC010000215         3636 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.217, whole genome shotgun sequence.
ACCESSION   DAHHSC010000215 DAHHSC010000000
VERSION     DAHHSC010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3636)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3636)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3636
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.217"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            9..806
                     /gene="fdhD"
                     /locus_tag="NBI57_002353"
     CDS             9..806
                     /gene="fdhD"
                     /locus_tag="NBI57_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HCD1823280.1"
                     /translation="MNKDVSLGQPIVRYEDGKLFNTTDQYVTEFPLTIMVNGEEFATV
                     ICSPTNLEELVIGFLASEGAILKRDELKSVLIDDSKGFAHVELNKDLGDRFQYSTKRM
                     IASCCGKSREFYFQNDAAIAKTSMSKITLTPIQIINMMTRLQSASHIYQETGGLHNAA
                     ISDGLTFFVHRQDIGRHNALDKLYGFCIQRHITVRDKVLIFSGRISSEILIKAAKIGV
                     GVILSKSAPTTLAVTLANDLNITAVGFIRNGGFNIYSHPERIIDSEQ"
     gene            complement(978..1748)
                     /locus_tag="NBI57_002354"
     CDS             complement(978..1748)
                     /locus_tag="NBI57_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCD1823281.1"
                     /translation="MASISMSDIYCNGTIFENDDEQLIYLTPSFPQRYTSNTWIYKKT
                     PTQERWLKDLERQHQLHTNQGSNHYAFSFPENEQLDNHWMAMFKDMNFELGIMELYAI
                     ESDALANLPRNSDVEIAVVDESHIDAYLKVAYQFSLPFGKDYADAHEEMVREHYQKDV
                     IKRLVAYLNNEPIGVVDVIESENYIELDGFGVLEQFRHQGIGSTIQSLIGEYAISKNH
                     KPIILVTDGEDTAKDMYAKQGYVYQSFCYQILKEDIGN"
     gene            complement(1825..2379)
                     /locus_tag="NBI57_002355"
     CDS             complement(1825..2379)
                     /locus_tag="NBI57_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HCD1823282.1"
                     /translation="MTTKQLVYTALMTAIIAILGMVPVIPLPFSSVPIVLQNIGIFLA
                     GVILGRKYGTLSVIVFLLLVVAGLPLLSGGRGGIGVFAGPSAGFLLLYPVVAFMIGAI
                     RDRFINEINFWILFVGILVFGVIALDVIGTLIMGMIINIPFTKAISISLAYLPGDILK
                     AIVASLIGTALLNHSQFRQIMGLK"
     gene            complement(2500..3441)
                     /locus_tag="NBI57_002356"
     CDS             complement(2500..3441)
                     /locus_tag="NBI57_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HCD1823283.1"
                     /translation="MTKKVYFNHDGGVDDLVSLFLLLQMENVQLIGVSTIGADCYLEP
                     SLSASVKIINRFSNEDIQVAPSYERGKNPFPKEWRMHAFFMDALPILNEPVKHVASNV
                     SDKEAFEDIIQTLKRQSEKVTLLFTGPLTDLAKALQKDSSIVQYIEKLVWMGGTFLPK
                     GNVEEPEHDGSAEWNAYWDPEAVKIVFDSDIEIDMVALESTNQVPLTLDVRQRWANER
                     QYTGIDFLGVSYAAVPPLTHFITNSTYFLWDVLTTAYIGNKDLVHSIEKKVDVISYGP
                     SQGKTFECKDGRKINVINHVDNNAFFDYITALAKKVN"
BASE COUNT         1201 a          674 c          523 g         1238 t
ORIGIN      
        1 gttagataat gaacaaagat gtatctttag gtcaaccaat tgttagatat gaagatggaa
       61 aattattcaa tacaacagat caatatgtga cagaatttcc acttacaatt atggtgaatg
      121 gtgaagaatt tgcgactgtt atatgcagtc caacaaacct tgaggagtta gtcattggat
      181 ttctggcttc ggaaggtgcc attttaaagc gagatgaatt aaaatctgtg ctgattgatg
      241 atagtaaagg ttttgcccat gttgaactta ataaagattt aggcgatcgc tttcaatatt
      301 ccacaaaacg aatgattgca tcttgttgtg gaaagagtcg tgagttttat tttcaaaatg
      361 atgctgctat cgctaaaact tctatgtcta aaatcacttt aactccaata caaattatta
      421 acatgatgac tcgattacaa agtgcaagtc atatttacca agaaacaggt ggcttacata
      481 atgctgctat aagcgatggt ttgacatttt ttgtacatcg acaagacatt ggtagacata
      541 acgctctaga taaattatat ggattttgta tccagcgaca tattactgtc cgagataaag
      601 tattaatttt tagtggacgt atttcatcag aaattttaat taaagccgcg aaaatcggtg
      661 ttggcgtcat tttatctaaa tcagctccga caacgcttgc tgtaacctta gctaatgacc
      721 taaatattac ggcagtaggt tttattcgaa atggtggctt taatatatat agtcatccag
      781 aacgtatcat cgactctgaa caataacata ttcattatag tgtgtcataa tgaacgatat
      841 tctacatgaa tcaacaattt aaacgcacta tttttataag tataagtagc tcaaaacaat
      901 ttaaaaagca gtaagttgat ttctactcga aaccagctta ctgctttttt attctaatac
      961 aaacctatca tcaatcgtta atttccaata tcttctttta atatttgata acaaaacgat
     1021 tgatagacat aaccttgctt tgcatacata tcttttgctg tatcttcacc atctgtaact
     1081 aatatgattg gtttgtgatt ttttgatatg gcgtattcac ctatcaacga ttgaattgta
     1141 gatccaattc cttggtgtcg aaattgttct aatacaccaa atccatctaa ttcaatgtaa
     1201 ttttcacttt caatgacatc tacaacgcca ataggttcat tatttaaata agctactaag
     1261 cgtttaatca catctttttg ataatgttcc cttaccattt cttcatgtgc atctgcatag
     1321 tcttttccaa atggcagact aaactgatat gcaactttta aataggcatc tatatgcgac
     1381 tcgtcaacga cggcaatttc aacgtcagag ttacgcggca aattagcaag cgcatcactt
     1441 tctatggcat acaattccat aatacctagt tcaaaattca tatctttaaa catagccatc
     1501 caatgattat caagttgttc attttccggg aaactaaacg cataatgatt tgaaccttga
     1561 tttgtatgta attgatgttg acgttctaag tctttcagcc atcgctcttg ggtaggcgtc
     1621 tttttatata tccatgtgtt gcttgtgtat cgttgtggaa aagaaggcgt taaataaatt
     1681 aactgctcgt cgtcattttc aaatatagtg ccgttacaat atatatctga catacttata
     1741 ctagccattg acacctactc ctttcacttt tataaatttc aaagcaactt attcaattac
     1801 tttattatct taacttaaat atgattattt taatcccata atttgacgaa actgcgagtg
     1861 attaagtaaa gctgtaccaa tcaaacttgc tacaattgct tttaatatat caccaggcaa
     1921 ataagctaat gaaattgaaa tagctttcgt aaatggtatg ttaataatca tgcccataat
     1981 caatgtacca ataacatcta atgctataac accaaaaact aaaataccaa cgaataaaat
     2041 ccagaaatta atttcattga tgaatctatc acgaatcgca ccaatcatga atgctacaac
     2101 tggatataat agtaaaaacc ctgctgaagg acctgcgaat acaccgatgc caccgcgacc
     2161 acctgataac aatggcaagc cagcaactac taataataaa aagacgataa cacttaatgt
     2221 gccatattta cgtcctaaaa tcacacctgc taagaaaata ccaatgtttt gaagtacaat
     2281 tggtactgaa gaaaatggta gtggaattac cggtaccatt cctaaaatag cgataatcgc
     2341 tgtcattaaa gctgtatata ctaactgttt tgttgtcatt ttttacttcc tttcgtattt
     2401 catgaccatt attatagcac cattgtaaac tttaagaaat gaaaagttta caataagttg
     2461 ttctatataa attaaaacct taattattct acacagctgt taatttactt ttttagcaag
     2521 tgcagttata taatcaaaaa atgcgttgtt atctacatga tttatgacat taattttgcg
     2581 cccatcttta cactcaaatg tcttaccttg acttggtcca taacttatta catcgacttt
     2641 tttctcaatt gaatgaacca agtccttgtt accaatataa gcagtcgtta aaacatccca
     2701 taaaaagtaa gtagaatttg ttataaagtg tgttaatggt ggtaccgctg cataacttac
     2761 tcctaaaaaa tcaatgcccg tatattggcg ttcattcgcc catctttgtc taacatctaa
     2821 cgttagcggc acttgattcg tactttctaa agcaaccata tcaatctcta tatcgctatc
     2881 aaaaacaatt ttaaccgctt ctggatccca atatgcattc cattctgcag aaccatcatg
     2941 ctcaggttct tcaacatttc cttttggtaa aaaggtgcca cccatccaaa ctaatttttc
     3001 tatatactga acgatagatg aatctttttg tagtgctttt gctaaatctg taagcgggcc
     3061 tgtaaataat aatgttactt tttctgattg tctctttaaa gtttgaataa tgtcttcaaa
     3121 ggcttctttg tcgctcacat ttgaagcaac atgtttgact ggctcattta aaattggcaa
     3181 tgcgtccata aaaaaggcat gcatacgcca ttctttagga aatggatttt ttcctcgttc
     3241 atatgatggc gcaacttgaa tatcttcatt tgaaaaacga ttaataattt ttactgatgc
     3301 gctcaaagat ggctctaaat aacaatcagc accaattgta ctgaccccta tcaattgaac
     3361 gttttccatt tgtaataata aaaatagaga tactaaatca tctacaccac catcatggtt
     3421 aaaataaact ttcttagtca ctcgaaatcc cccttacatt tcattgtcca ttaacaatat
     3481 tatgttttta taaaacgttg ttatatatct ttctacaaaa tcataacatt ctgttgtaat
     3541 ccttttaaac ctcttctttt attgatagcc attccctgat atcccttcca taaagtatgc
     3601 actaatataa ttatgatata acttatcaat aaagag
//
LOCUS       DAHHSC010000216         3542 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.219, whole genome shotgun sequence.
ACCESSION   DAHHSC010000216 DAHHSC010000000
VERSION     DAHHSC010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3542)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3542)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3542
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.219"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            complement(<1..971)
                     /locus_tag="NBI57_002357"
     CDS             complement(<1..971)
                     /locus_tag="NBI57_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HCD1823284.1"
                     /translation="MSNTQKKNVPELRFPGFEGEWEEKQLGDLTDRVIRKNKNLESKK
                     PLTISGQLGLIDQTEYFSKSVSSKNLENYTLIKNGEFAYNKSYSNGYPLGAIKRLTRY
                     DSGVLSSLYICFSIKSEMSKDFMEAYFDSTHWYREVSGIAVEGARNHGLLNVSVNDFF
                     TILIKYPSLEEQQKIGKFFSKLDRQIELEEQKLELLQQQKKGYMQKIFSQELRFKDEN
                     GEDYPDWENSKIEKYLKERNERSDKGQMLSVTINSGIIKFSELDRKDNSSKDKSNYKV
                     VRKNDIAYNSMRMWQGASGKSNYNGIVSPAYTVLYPTQNTSSLFIGY"
     gene            complement(964..2520)
                     /locus_tag="NBI57_002358"
     CDS             complement(964..2520)
                     /locus_tag="NBI57_002358"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000028656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HCD1823285.1"
                     /translation="MSITEKQRQQQAELHKKLWSIANDLRGNMDASEFRNYILGLIFY
                     RFLSEKAEQEYADALSGEDITYQEAWADEEYREDLKAELIDQVGYFIEPQDLFSAMIR
                     EIETQDFDIEHLATAIRKVETSTLGEESENDFIGLFSDMDLSSTRLGNNVKERTALIS
                     KVMVNLDDLPFVHSDMEIDMLGDAYEFLIGRFAATAGKKAGEFYTPQQVSKILAKIVT
                     DGKDKLRHVYDPTCGSGSLLLRVGKETQVYRYFGQERNNTTYNLARMNMLLHDVRYEN
                     FDIRNDDTLENPAFLGNTFDAVIANPPYSAKWTADSKFENDERFSGYGKLAPKSKADF
                     AFIQHMVHYLDDEGTMAVVLPHGVLFRGAAEGVIRRYLIEEKNYLEAVIGLPANIFYG
                     TSIPTCILVFKKCRQQDDNVLFIDASNDFEKGKNQNHLSDAQVERIIDTYKRKETIDK
                     YSYSATLQEIADNDYNLNIPRYVDTFEEEAPIDLDQVQQDLKNIDKEIAEIEQEINAY
                     LKELGVLKDE"
     gene            2598..2699
                     /locus_tag="NBI57_002359"
     CDS             2598..2699
                     /locus_tag="NBI57_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1823286.1"
                     /translation="MENFDVGGGIFIFMEIGIGFMTHKQKRLNDDID"
     gene            complement(2784..3467)
                     /locus_tag="NBI57_002360"
     CDS             complement(2784..3467)
                     /locus_tag="NBI57_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superantigen-like protein SSL10"
                     /protein_id="HCD1823287.1"
                     /translation="MKFTALAKATLALGILTTGTLTTEVHSGHAKQNQKSVNKHDKEA
                     LYRYYTGKTMEMKNISALKHGKNNLRFKFRGIKIQVLLPGNDKSKFQQRSYEGLDVFF
                     VQEKRDKHDIFYTVGGVIQNNKTSGVVSAPILNISKEKGEDAFVKGYPYYIKKEKITL
                     KELDYKLRKHLIEKYGLYKTISKDGRVKISLKDGSFYNLDLRSKLKFKYMGEVIESKQ
                     IKDIEVNLK"
BASE COUNT          984 a          683 c          519 g         1356 t
ORIGIN      
        1 ttatatccaa taaataatga gctagtattt tgtgttggat aaagcacagt atatgcaggg
       61 ctaacaatcc cattataatt tgatttacca ctagcccctt gccacattct catagaatta
      121 tatgcaatat catttttcct aactacttta taattacttt tatctttact tgaattatct
      181 tttctatcca attcactaaa ttttataatg ccactattta tagttactga aagcatttgc
      241 cctttgtcag aacgttcgtt tctctctttt aaatattttt ctattttgct attttcccaa
      301 tctggataat cttcaccatt ctcatctttg aatcgcagtt cctgtgagaa aattttctgc
      361 atatagcctt ttttctgttg ttgaagtaat tcaagctttt gttcttctaa ttcaatttgt
      421 cggtcgagtt tgctgaagaa cttgcctatt ttttgctgtt cttctaaact tggatattta
      481 attagaatag taaaaaaatc attcacagaa acatttaata atccgtgatt tcttgcaccc
      541 tcaactgcaa ttccagaaac ttctctatac cagtgtgtcg aatcaaaata tgcttccatg
      601 aagtctttag acatttcact tttaatagaa aaacaaatat acaaagagga caatacacca
      661 ctatcatatc tagttaatct tttaatagcc cctaatgggt atccattaga ataacttttg
      721 ttatacgcga attctccatt ctttattagt gtataatttt ctagattttt cgacgaaact
      781 gatttactaa aatattctgt ttgatcaatt aaacctaact gtccggatat tgttaaaggc
      841 tttttcgatt ctaagttttt atttttccta attactctat ctgtaagatc ccccaactgc
      901 ttctcttccc attcgccttc aaaccctggg aatctcaact ctggcacatt tttcttttgt
      961 gtattactca tctttcaaca ccccaagttc tttcaggtat gcattgattt cttgttcaat
     1021 ttctgcgatt tctttgtcga tatttttcaa atcttgttgg acttgatcta aatcaattgg
     1081 cgcttcttct tcgaatgtat cgacatacct cggtatgttt aggttgtaat cgttatcggc
     1141 aatctcttgt aatgtcgcac tgtaactgta tttatcaatt gtttctttac gcttgtatgt
     1201 gtcaataata cgttcgactt gggcatcgct taaatggttt tgattttttc ctttttcaaa
     1261 atcattggat gcatcgataa ataatacgtt gtcgtcttgt tggcgacatt ttttaaatac
     1321 taagatacat gttggaatac ttgtcccata gaaaatattc gctggtaagc caatcacggc
     1381 ttctaagtag ttcttttctt caattaaata acgacgaatg acgccttctg cggcaccacg
     1441 gaataagaca ccatgtggga gtacaactgc catggtacct tcatcgtcta ggtaatgtac
     1501 catatgttga ataaaggcaa agtctgcttt ggattttggc gcaagcttgc cgtaaccact
     1561 gaagcgttca tcattttcaa attttgaatc tgcagtccat ttcgcactgt atggtgggtt
     1621 cgctataacc gcatcaaatg tattgcctaa gaaagctgga ttttccaacg tatcatcatt
     1681 acggatatca aagttttcat agcgcacatc atgtaataac atgttcatgc gcgctaagtt
     1741 gtaagtggta ttgttacgtt cttgaccgaa ataacgatac acttgcgttt ctttaccaac
     1801 acgtaacaat aatgaacctg aaccacatgt tgggtcatac acgtgacgta atttatcttt
     1861 accgtctgtg acaatcttcg ccagtatctt agatacttgt tgtggtgtat agaactcgcc
     1921 tgctttttta cctgctgtcg ccgcaaagcg cccgataaga aattcgtatg catcacctaa
     1981 catatcaatt tccatatcac tgtgaacgaa tggtaagtcg tcaaggttaa ccataacttt
     2041 ggaaattagt gcagtacgtt ctttgacatt gttacctagt cgcgttgaac ttaagtccat
     2101 atcgctgaac agtccgataa agtcattttc actttcttca cctagtgttg atgtttcaac
     2161 tttacgaatc gccgttgcga gatgttcgat atcgaaatct tgcgtttcaa tttcacgaat
     2221 catcgcactg aataaatctt gtggttcaat gaagtaaccg acttgatcaa ttaattcagc
     2281 ttttaagtct tcacgatatt cttcatctgc ccatgcttct tgatacgtaa tatcttcacc
     2341 tgacaaggca tctgcatatt cttgttccgc tttttcagat aagaagcgat agaaaatcaa
     2401 gcctaaaatg taattacgga attcactcgc atccatattc cctcttaaat cattcgcaat
     2461 cgaccataat tttttatgta attcagcttg ttgctgacgt tgtttttcag taatagacat
     2521 gtgattcctc cgcctttgaa taagtaattt atctctttgt gtaatagatt tattataaca
     2581 tttggttatg ttgcgatgtg gagaattttg atgttggtgg tggaattttt atattcatgg
     2641 agattgggat aggattcatg acacacaaac aaaaacggct taacgacgat atcgattgat
     2701 gcatcctttt tgtataagct atgcgcacca atttacattt caccgttaag ccgcttcaat
     2761 tactttttat tattcgtaat tatttacttt aagttaactt caatatcttt aatttgtttg
     2821 ctttctatga cttcccccat atatttaaat tttaatttag atcttaaatc aaggttataa
     2881 aaactgccat ctttcaagct aattttgacc ctaccatctt ttgagattgt tttataaagt
     2941 ccgtattttt caattagatg ctttctcaac ttataatcca gctcttttag tgttattttt
     3001 tcttttttaa tgtaataagg gtaacctttc acaaaagcat cttcaccctt ttcttttgaa
     3061 atatttaata ttggtgcact gacaactcca gatgttttat tattctgtat tacaccacca
     3121 acagtataaa atatatcgtg cttatctctt ttttcttgaa caaaaaacac atctaacccc
     3181 tcataactac gctgttgaaa tttactttta tcatttccag gcagtaaaac ttgaatctta
     3241 atacctctaa acttaaaacg caagttattt ttaccatgtt tcaaagcact aatatttttc
     3301 atttccatag tctttccagt gtagtatcgg tatagtgcct ccttgtcatg tttatttact
     3361 gacttttgat tttgttttgc atgacctgaa tgaacttctg ttgttaaagt tcctgttgtt
     3421 aaaattccta aagctaatgt tgcttttgct aatgctgtaa atttcatatt tatttgctcc
     3481 aatcttaata tattggattg ttttcattac gtaatttgaa tcatacattt ttattataac
     3541 gt
//
LOCUS       DAHHSC010000217         3476 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.220, whole genome shotgun sequence.
ACCESSION   DAHHSC010000217 DAHHSC010000000
VERSION     DAHHSC010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3476)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3476)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3476
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.220"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            complement(170..343)
                     /locus_tag="NBI57_002361"
     CDS             complement(170..343)
                     /locus_tag="NBI57_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NETI motif-containing protein"
                     /protein_id="HCD1823288.1"
                     /translation="MKFKVNENETIADCLSRMKMQGYMPVKRIEKPVFQEQKDGTVEV
                     SHQEIVFVGKKIQ"
     gene            complement(324..926)
                     /locus_tag="NBI57_002362"
     CDS             complement(324..926)
                     /locus_tag="NBI57_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HCD1823289.1"
                     /translation="MSFVTENPWLMVLTIFIINVCYVTFLTMRTILTLKGYRYIAASV
                     SFLEVLVYIVGLGLVMSNLDHIQNIIAYAFGFSIGIIVGMKIEEKLALGYTVVNVTSA
                     EYELDLPNELRNLGYGVTHYAAFGRDGSRMVMQILTPRKYERKLMDTIKNLDPKAFII
                     AYEPRNIHGGFWTKGIRRRKLKDYEPEELESVVEHEIQSK"
     gene            complement(1197..2018)
                     /gene="nadE"
                     /locus_tag="NBI57_002363"
     CDS             complement(1197..2018)
                     /gene="nadE"
                     /locus_tag="NBI57_002363"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HCD1823290.1"
                     /translation="MSKLQDVIVQEMKVKKRIDSAEEIMELKQFIKNYVQSHSFIKSL
                     VLGISGGQDSTLVGKLVQMSVNELREEGIDCTFIAVKLPYGVQKDADEVDQALRFIEP
                     DEIVTVNIKPAVDQSVQSLKEAGIVLTDFQKGNEKARERMKVQFSIASNRQGIVVGTD
                     HSAENITGFYTKYGDGAADIAPIFGLNKRQGRQLLAYLGAPKELYEKTPTADLEDDKP
                     QLPDEDALGVTYEAIDNYLEGKPVTPEEQKVIENHYIRNAHKRELAYTRYTWPKS"
     gene            complement(2011..>3476)
                     /locus_tag="NBI57_002364"
     CDS             complement(2011..>3476)
                     /locus_tag="NBI57_002364"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500623.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HCD1823291.1"
                     /translation="QLEDDSLMLHNDLYQINMAESYWNDNIHEKMAVFDLYFRKMPFN
                     SGYAVFNGLKRVIDFIEHFGFSESDLEYLKSIGYKDDFLSYLKDLKFTGSIRSMQEGE
                     LCFGNEPLLRVEAPLIQAQLIETILLNIVNFHTLITTKASRIRQIASNDKLMEFGTRR
                     AQEIDAALWGARAAYIGGFDSTSNVRAGKLFGIPVSGTHAHAFVQTYGDEYVAFKKYA
                     ERHKNCVFLVDTFHTLKSGVPNAIKVAKELGDKINFVGIRLDSGDIAYLSKEARRMLD
                     EAGFTETKIIASNDLDEETITSLKAQGAKVDSWGVGTKLITGYDQPALGAVYKLVAIE
                     NEEGSYSDRIKLSNNAEKVTTPGKKNVYRIINKKTGKAEGDYITLENENPYDEQPLKL
                     FHPVHTYKMKFIKSFEAIDLHHNIYENGKLVYQMPTEDESREYLAQGLQSIWDENKRF
                     LNPQEYPVDLSKACWDNKHKRIFEVAEHVKEMEEDNE"
BASE COUNT         1077 a          683 c          457 g         1259 t
ORIGIN      
        1 aaaatattaa gaacccccag catatgcttt atatagcttg ctgggggttt ctcatacaat
       61 atggatcgag catcttgcat gtataaacaa ataggctatt caaagtaaaa ttactcaaac
      121 tttgaatagc ctttcctctt caaatagtct ttatttaaat tggattatgt tattggattt
      181 tcttacctac aaaaacgatt tcttgatgtg atacttcaac cgtgccatct ttttgctctt
      241 gaaacacggg tttttcaata cgttttaccg gcatataccc ttgcattttc attcgtgaca
      301 aacaatcagc aattgtttca ttctcattta ctttgaattt catgttctac tacactttcc
      361 agttcttctg gttcataatc tttaagcttt ctacgacgaa tgcctttagt ccagaatcca
      421 ccatgtatgt ttcgaggttc atacgcaatg ataaatgctt tcggatctaa attttttatc
      481 gtatccatca atttacgttc atattttctt ggtgttaaaa tttgcatcac catacgacta
      541 ccatctctac caaacgcagc atagtgcgta acgccatatc ctaaatttcg aagttcattc
      601 ggtaaatcta actcatattc tgctgaagtt acatttacaa ctgtataacc taatgccagt
      661 ttttcttcta ttttcatacc aacaatgata cctattgaaa aaccaaatgc gtaggcaata
      721 atattttgaa tatggtctaa attagacata accaaaccta aaccaacgat ataaactaat
      781 acttctaaaa aactaactga tgcagcaata taacgataac ctttcaacgt taaaattgtt
      841 cgcatcgtta aaaacgttac ataacaaacg ttaatgataa atatagttag taccattaac
      901 catggatttt ctgttacgaa tgacataagg cgccccactc tctttcaatt acactttaaa
      961 taccaaactg cttcaaccta accttaatga atgcatatta atataatata gtacgaaaac
     1021 tctcataaat aaatagcgaa tttgtcataa ataaaaattt attctcaaat gaacatactt
     1081 actttagttc atacactttc aatttataca ctgaagtagt ctagctgaat gtggtttaat
     1141 gttattaatt ctaactcata tttaatttaa gtcacacgtt agaagaaaaa attaaattag
     1201 gattttggcc acgtgtatct tgtatatgca agttcacgtt tgtgtgcatt tcgtatataa
     1261 tgattttcaa ttactttttg ttcttctggc gtaactggct taccttctaa ataattatca
     1321 atcgcctcat aagttacacc taatgcatct tcatctggaa gctgtggttt atcatcttct
     1381 aaatcagcag ttggcgtttt ttcatataat tcctttggcg caccaagata cgctaataat
     1441 tgacgacctt gtcgtttatt caaaccaaat ataggtgcga tatctgcagc accatcaccg
     1501 tacttcgtat aaaacccagt tatattttca gctgaatgat ctgttcctac tacaatacct
     1561 tgtcggtttg aagcaattga aaattgtact ttcatacgtt cacgcgcttt ttcatttcct
     1621 ttttggaaat ctgtaagaac aataccagct tcttttaatg attgcacact ttgatcaact
     1681 gcaggcttaa tattgactgt tactatttca tctggttcaa tgaatcgcaa agcttggtca
     1741 acttcatcag catctttttg aactccataa ggtaatttaa ctgcaataaa cgtacaatca
     1801 atgccttctt cacgtaattc gttaacagac atttgtacta gttttccaac taatgtagaa
     1861 tcttgtcctc ctgaaatacc taacactaaa gattttataa atgaatgtga ttgtacataa
     1921 ttttttataa attgctttaa ttccataatt tcttcagcac tatcgatacg ctttttcact
     1981 ttcatttctt gtacaataac gtcttgtaat ttactcatta tcttcttcca tctccttaac
     2041 gtgttccgca acttcaaaaa tacgtttatg tttattatcc caacatgcct tgcttaaatc
     2101 gactggatat tcttgtggat taaggaaacg cttattttca tcccaaatag attgtaatcc
     2161 ttgtgctaaa tattcacgtg attcatcttc tgttggcatt tgatatacta atttaccatt
     2221 ttcataaata ttatgatgca aatcaatggc ttcgaaagat tttataaatt tcattttata
     2281 agtatgcact ggatggaata attttaaagg ttgttcatcg tatggatttt cattttccaa
     2341 agtaatataa tcgccttctg ccttacctgt tttcttgttt ataatgcgat atacattttt
     2401 cttacctggc gtcgtaacct tttcagcgtt atttgataat ttaatacgat cactatatga
     2461 accctcttca ttttcaatag ctacaagttt atatactgca cctaatgctg gttgatcgta
     2521 tcctgtaatc agctttgtac caacgcccca agaatctact tttgcacctt gtgctttcaa
     2581 actcgtaatc gtttcttcat ccaaatcatt agacgcgata attttagttt cagtaaatcc
     2641 tgcttcatca agcatacgtc ttgcctcttt agataaataa gcgatatctc cagaatccaa
     2701 tcgaatacct acaaagttaa ttttgtcacc taattctttt gcaactttta ttgcatttgg
     2761 cacgccagat tttaaagtat ggaatgtatc tactaggaac acacaatttt tatgtctttc
     2821 agcatatttt ttgaaggcaa catattcatc tccataagtt tggacaaatg cgtgtgcatg
     2881 tgtaccagac acaggtatac caaataattt acctgcccta acattacttg tagaatcaaa
     2941 gcccccgatg taagcagctc tagcgcccca caatgctgca tcaatttctt gcgcacgacg
     3001 tgtaccaaac tccattaatt tatcatttga tgcaatttga cgaattctac tagcctttgt
     3061 tgtaattagt gtatggaaat ttacaatgtt taataaaatt gtttctatta attgcgcttg
     3121 aatcaatggt gcttctacgc gtaacaatgg ttcgttacca aagcataatt cgccttcttg
     3181 catcgaacga atgcttcctg tgaattttaa atcttttaaa tatgataaga aatcatcctt
     3241 gtagccaata gactttaaat attccaaatc agattctgaa aatccaaaat gttctataaa
     3301 atcaatgacg cgttttaaac cattaaaaac agcatagcca ctattaaatg gcatttttct
     3361 aaaatacaaa tcaaatacag ccattttttc atgaatatta tcattccaat aactttcagc
     3421 catatttatt tgatataagt cattatgtaa cattaaactg tcgtcttcta attggt
//
LOCUS       DAHHSC010000218         3450 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.221, whole genome shotgun sequence.
ACCESSION   DAHHSC010000218 DAHHSC010000000
VERSION     DAHHSC010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3450)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3450)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3450
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.221"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            <1..2058
                     /locus_tag="NBI57_002365"
     CDS             <1..2058
                     /locus_tag="NBI57_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HCD1823292.1"
                     /translation="EKSNDEIVQNTINKRINFIFGVIVFIFAVLVLRLGYLQIAQGSH
                     YKQIIKNDENITVNESVPRGRILDRNGKVLVDNASKMAITYTRGRKTTQSEMLDTAEK
                     LSKLIKMDTKKITERDKKDFWIQLHPKKAKAMMTKEQAMLADGSIKQDQYDKQLLSKI
                     GKSQLDELSSKDLQVLAIFREMNAGTVLDPQMIKNEDVSEKEYAAVSQQLSKLPGVNT
                     SMDWDRKYPYGDTLRGIFGDVSTPAEGIPKELTEHYLSKGYSRNDRVGKSYLEYQYED
                     VLRGKKKEMKYTTDKSGKVTSSEVLNPGARGQDLKLTIDIDLQKEVEALLDKQIKKLR
                     SQGAKDMDNAMMVVQNPKNGDILALAGKQINKSGKMTDYDIGTFTSQFAVGSSVKGGT
                     LLAGYQNKAIKVGETMVDEPLHFQGGLTKRSYFNKNGHVSINDKQALMHSSNVYMFKT
                     ALKLAGDPYYSGMALPSDISSPAQKLRRGLNQVGLGVKTGIDLPNETRGQIEPLTNNP
                     GNYLDLSIGQYDTYTPLQLSQYVSTIANDGYRIQPHIGLTIHESTNKDEVGPLKKKIN
                     GTVLNKVNNTEKEIKQIQEGFKMAFNDKDGTGYVSFKDTVVPTAGKTGTAEVFQNGEP
                     RVNSTYIGYAPIDDPKLAFSIVYTNQPVPPPWLTGGDLGRDVINYYFKQLGKDDKNKD
                     KDK"
     gene            2171..2320
                     /gene="rpmG"
                     /locus_tag="NBI57_002366"
     CDS             2171..2320
                     /gene="rpmG"
                     /locus_tag="NBI57_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001265708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HCD1823293.1"
                     /translation="MRVNVTLACTECGDRNYITTKNKRNNPERVEMKKFCSRENKQTL
                     HRETK"
     gene            2529..3068
                     /locus_tag="NBI57_002367"
     CDS             2529..3068
                     /locus_tag="NBI57_002367"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HCD1823294.1"
                     /translation="MTKNEIRKYILHKMKNFNKAEKRKADTWLRNQFFATEEYKEANA
                     IALVLSFNHEVDTFSIIEQALMDHKRIFVPKMDYLNHQMTFKEIFNLKDIDVDNKGIY
                     YPTSKGETTNNLDLIVVPGVGFQDDGYRIGYGGGYYDRFLANYQTKTISLLYDFQITS
                     FEPESFDQPVDKLIIYQSA"
     gene            3080..>3450
                     /locus_tag="NBI57_002368"
     CDS             3080..>3450
                     /locus_tag="NBI57_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001652331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HCD1823295.1"
                     /translation="MNIDKQFWKTIYYWIRYLNFDIVSREKDDQEIWLAHKRKKQVVI
                     FKQHIKSTQEIRFDKAKVLEHKDEIANFISFEPQSFEFYYFTESEFSEEQLNEVSPIR
                     IKFNVIRHTKDLIKHMPNIFLA"
BASE COUNT         1365 a          470 c          621 g          994 t
ORIGIN      
        1 gaaaaatcaa atgatgaaat cgttcaaaat acaattaaca agagaattaa ctttatattt
       61 ggtgtgattg tatttatttt tgcagtacta gtactacgtt taggttattt acaaatcgca
      121 caaggctcac attataaaca aattataaaa aatgatgaaa acattacagt gaatgagtct
      181 gtgccaagag gtcgtatttt agacagaaat gggaaagttt tagttgataa tgcttctaaa
      241 atggctatta catatactag gggtcgaaaa acaacacaat cggaaatgtt ggatacggct
      301 gaaaagttat caaagctaat caagatggat actaagaaaa ttacagaacg tgataagaaa
      361 gatttctgga ttcagttgca tcctaaaaaa gcaaaagcaa tgatgacaaa agaacaagct
      421 atgttagcag atggaagtat taaacaagat caatatgata aacaactgtt atcgaaaatc
      481 ggaaaatcac aattagatga attgtcttct aaagatttac aagttttagc tatttttcga
      541 gagatgaatg caggaacagt tttagatcca caaatgataa aaaatgaaga tgtcagtgaa
      601 aaagagtatg cagcagtttc tcagcaactt tccaaattac caggtgttaa cacgtctatg
      661 gattgggata gaaaatatcc atatggcgat actttaagag gtatattcgg agatgtatcg
      721 acacctgctg aaggtattcc aaaagaattg acagaacatt acttatccaa aggatattca
      781 cgcaatgatc gtgttggaaa atcttaccta gaatatcaat atgaagatgt attgcgtggt
      841 aagaagaaag aaatgaaata cacaacggac aaatctggta aagttacatc ttcagaagtg
      901 ttaaatcctg gcgctcgcgg tcaagatttg aaattaacga tcgatataga tcttcaaaaa
      961 gaagtagaag cattattaga taaacaaatt aagaagcttc gcagccaagg tgcaaaagat
     1021 atggataatg ctatgatggt tgtacaaaat cctaaaaatg gagacattct tgcgcttgcc
     1081 ggaaagcaga ttaataagag tggtaaaatg actgattatg acattggtac gtttacttct
     1141 caatttgcgg ttggatcttc tgtaaaaggt ggaacattat tagctggtta tcagaataaa
     1201 gctatcaaag ttggagaaac aatggtcgat gaaccattac atttccaagg tggtttgaca
     1261 aaacgatcat acttcaataa aaacgggcat gtatctatta atgataagca agctttgatg
     1321 cattcatcaa acgtatatat gtttaaaaca gcattaaaat tagcgggaga cccttattat
     1381 tctggtatgg ctttaccttc agatataagt tcacctgccc aaaagcttag aagaggatta
     1441 aatcaagtag gcttaggtgt gaaaacaggg atagatttac caaatgaaac aagaggtcaa
     1501 atcgaaccat taacaaataa tccaggtaat tatctagatt tatcaattgg tcaatatgat
     1561 acctatacac cattacaatt atcacaatat gtttcaacta tagcgaatga tggttataga
     1621 atacagccac acattggatt aacgattcat gaatcaacta ataaagatga ggttggtcca
     1681 ctcaagaaga aaattaatgg cactgtcttg aacaaggtta ataatactga aaaggaaatc
     1741 aaacaaattc aagaaggatt caaaatggca tttaatgata aagatggtac tggatatgtt
     1801 agttttaaag atacagtagt acctactgct ggtaaaacgg gtaccgctga agtgttccaa
     1861 aacggagagc caagagttaa ctctacttat ataggatacg cgccaattga tgatccaaaa
     1921 ttagcgtttt caattgtata tacaaatcag cctgtaccac caccatggtt aacaggtgga
     1981 gacttaggta gagatgtaat taactactac tttaagcagt taggtaaaga tgataaaaat
     2041 aaagacaaag acaaataaaa tttaacctga cgattgtgta gcgcatggtt gtaaaatttt
     2101 aactttgcaa atattataga tgttggtata ataataaagt cgtatttaga aatgataagg
     2161 aggaataacc atgcgcgtaa acgtaacttt agcttgtacg gaatgtggtg acagaaacta
     2221 cattacaact aagaacaaaa gaaataatcc agaacgtgtt gaaatgaaga aattctgttc
     2281 acgtgaaaac aaacaaactt tacaccgtga aacaaaataa tcattaaaat taaatttaaa
     2341 ttaacaataa aaaaggtgaa atctcaaaat aagaggtttc acctttttaa ttatgcacac
     2401 gtatgaaatg ataaaagatt ttcaattgaa ataataaatt ttagaattgt cgcataattc
     2461 atcatgacaa cataatgaca tgtattgtta ttaacgatat aatagaattg aataaagtgg
     2521 gtgataatgt gactaaaaat gagattagaa aatacatttt acataaaatg aagaatttta
     2581 ataaagctga aaagcgaaaa gcagacacat ggttaagaaa tcaatttttt gcaactgaag
     2641 aatacaaaga agcaaacgca attgcgctag ttctttcttt taatcatgaa gtagatactt
     2701 tttctattat tgaacaagcc ttaatggatc ataaacgtat ttttgtaccg aaaatggatt
     2761 atttaaatca tcaaatgact tttaaagaaa tatttaatct caaagatatt gatgtcgata
     2821 ataaggggat ttactatcca acttcaaaag gtgaaacaac gaataaccta gatttaattg
     2881 ttgttcctgg tgttggattt caagacgatg gatatagaat tgggtatggt ggtggctatt
     2941 acgacaggtt tttagctaat tatcagacaa agacaataag cttattatac gattttcaaa
     3001 taacatcatt tgaaccggaa tcattcgatc aaccagtcga taaattgatt atatatcaat
     3061 cagcatagtg gaggactaaa tgaacataga caaacaattt tggaaaacaa tatattattg
     3121 gattaggtac ttgaattttg atattgtcag tagggaaaaa gatgaccaag aaatttggtt
     3181 agcccataaa agaaaaaaac aagtagtcat ttttaaacaa catatcaagt ctacccaaga
     3241 aatacgtttt gacaaagcga aagtgcttga acacaaagat gaaatagcaa attttatttc
     3301 tttcgaacca caaagttttg aattttatta ttttacagaa tcggaatttt cagaagaaca
     3361 attaaatgaa gtttcgccaa ttagaattaa attcaatgtt ataagacaca caaaagattt
     3421 gataaagcat atgccgaata tatttttggc
//
LOCUS       DAHHSC010000219         3415 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.222, whole genome shotgun sequence.
ACCESSION   DAHHSC010000219 DAHHSC010000000
VERSION     DAHHSC010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3415)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3415
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.222"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            131..502
                     /locus_tag="NBI57_002369"
     CDS             131..502
                     /locus_tag="NBI57_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3899 domain-containing protein"
                     /protein_id="HCD1823296.1"
                     /translation="MLKKWLGMALITPILTFIIWVFNSHTIITYLNILFYVSLIIFIS
                     IFLILLVQEGIFDATSYGFRRLKYQMSSSKKKKSISDDPFFNPQEVKKEHYFVSTWII
                     PLLLINILYFIMTIVLSLILV"
     gene            complement(557..1801)
                     /gene="fabF"
                     /locus_tag="NBI57_002370"
     CDS             complement(557..1801)
                     /gene="fabF"
                     /locus_tag="NBI57_002370"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HCD1823297.1"
                     /translation="MSQNKRVVITGMGALSPIGNDVKTTWENALKGVNGIDKITRIDT
                     EPYSVHLAGELKNFNIEDHIDKKEARRMDRFTQYAIVAAREAVKDAQLDINDNTADRI
                     GVWIGSGIGGMETFEIAHKQLMDKGPRRVSPFFVPMLIPDMATGQVSIDLGAKGPNGA
                     TVTACATGTNSIGEAFKIVQRGDADAMITGGTEAPITHMAIAGFSASRALSTNDDIET
                     ACRPFQEGRDGFVMGEGAGILVIESLESAQARGANIYAEIVGYGTTGDAYHITAPAPE
                     GEGGSRAMQAAMDDAGIEPKDVQYLNAHGTSTPVGDLNEVKAIKNTFGEAAKHLKVSS
                     TKSMTGHLLGATGGIEAIFSALSIKDSKVAPTIHAVTPDPECDLDIVPNEAQDLDITY
                     AMSNSLGFGGHNAVLVFKKFEA"
     gene            complement(1813..2754)
                     /locus_tag="NBI57_002371"
     CDS             complement(1813..2754)
                     /locus_tag="NBI57_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HCD1823298.1"
                     /translation="MNVGIKGFGAYAPEKIIDNAYFEQFLDTSDEWISKMTGIKERHW
                     ADDDQDTSDLAYEASVKAIADAGIQPEDIDMIIVATATGDMPFPTVANMLQERLGTGK
                     VASMDQLAACSGFMYSMITAKQYVQSGDYHNILVVGADKLSKITDLTDRSTAVLFGDG
                     AGAVIIGEVSEGRGIISYEMGSDGTGGKHLYLDKDTGKLKMNGREVFKFAVRIMGDAS
                     TRVVEKANLTSDDIDLFIPHQANIRIMESARERLGISKDKMSVSVNKYGNTSAASIPL
                     SIDQELKNGKLKDDDTIVLVGFGGGLTWGAMTIKWGK"
     gene            3049..3234
                     /locus_tag="NBI57_002372"
     CDS             3049..3234
                     /locus_tag="NBI57_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzD family protein"
                     /protein_id="HCD1823299.1"
                     /translation="MKHLVTFFWALLLMQMVNFVLNSLVGGDSLNVVNPIIMAVLFTI
                     FTAIFAAVIKPPKDSSQ"
BASE COUNT         1061 a          653 c          508 g         1193 t
ORIGIN      
        1 tgctttaaac taatgaagaa tgattgattt tagaatttca ataaagctgc agaaattaaa
       61 aaatcattcc tcaatgtctc attttaatgt aaactatgcg taagagtttt ctataagtgg
      121 gagtgatcaa atgttgaaaa aatggcttgg aatggcacta ataacgccaa tattaacgtt
      181 tattatatgg gtttttaata gccatactat cattacatat ttaaatatat tgttttatgt
      241 ttcattaatt atttttatta gcatattttt gattttactt gtacaagaag gcatttttga
      301 tgcgacaagt tatggtttta gaagattaaa gtatcaaatg tcatcttcga aaaagaagaa
      361 gtctatctct gatgatccat ttttcaaccc acaagaggta aaaaaagagc attactttgt
      421 ttctacatgg ataatcccct tattattaat aaatattcta tattttatta tgacgattgt
      481 gctgtcgctc atcttagtat aaagaaacag aaaaaatctt cagtaactaa aacaacgtta
      541 ctgaagattt ttataattat gcttcaaatt tcttgaatac taatactgcg ttatgtccac
      601 cgaatcctaa gctattactc attgcataag taatatcaag gtcttgcgct tcatttggaa
      661 caatatccaa atcacattct gggtctggtg ttaccgcatg tattgtcggt gcgactttag
      721 agtctttaat tgaaagcgct gagaagattg cttcaattcc acctgttgca ccaagtaagt
      781 gaccagtcat tgattttgtt gagctaactt ttaagtgttt agctgcttca ccaaatgtat
      841 ttttaatagc tttaacttca tttaagtcac caacaggagt acttgtacca tgggcattta
      901 agtattgtac atctttaggt tcaataccag catcatccat agctgcttgc attgctctag
      961 aaccgccttc accttctgga gctggcgctg taatatgata agcatcacct gtagtaccat
     1021 agccaactat ctcagcataa atattggcac ctcgagcttg tgctgattct aaagattcga
     1081 ttactaaaat accagcacct tcacccataa caaaaccgtc tctaccttct tggaatggac
     1141 gacatgctgt ttcaatgtca tcatttgtag aaagcgctcg acttgcactg aaacctgcaa
     1201 ttgccatatg agtgattgga gcttccgtac caccagtaat cattgcatct gcatcaccgc
     1261 gttgcacaat tttaaatgct tctccgattg agtttgtacc tgttgcacat gctgtaactg
     1321 ttgcaccatt tggtcctttt gcacctaagt caattgatac ttgcccagtt gccatatcag
     1381 gaattaacat tggtacgaaa aatggactca cacgtcttgg gcctttatcc attaattgtt
     1441 tatgtgcaat ttcaaatgtt tccataccac cgataccaga accaatccat acaccgattc
     1501 gatctgcagt attatcattg atatctaatt gcgcatcttt aacagcctct ctagctgcta
     1561 caattgcata ttgagtaaat ctatccatac gacgcgcttc ttttttgtcg atatgatctt
     1621 caatattaaa gtttttaagt tctcctgcta agtgaacgct ataaggttca gtatcgatac
     1681 gtgtaatttt atcgatacca tttacgcctt ttagagcatt ctcccatgtt gttttgacat
     1741 cattaccgat tggagaaagg gctcccatac ctgtaataac tactctttta ttttgactca
     1801 ttcgttatcc tcctattttc cccattttat tgtcattgcg ccccaagtta ggccgccacc
     1861 gaatccgaca agaacaattg tatcatcatc tttgagttta ccatttttta attcttgatc
     1921 gatacttaaa ggtattgacg cagctgaagt atttccatat ttatttacag aaacactcat
     1981 tttgtctttt gaaataccta agcgttctct agctgattcc ataattctaa tattagcttg
     2041 atgaggaata aataaatcta tatcatctga tgttaaattc gctttttcaa ctacacgtgt
     2101 tgatgcatca cccataattc taacagcaaa tttaaatact tctcgaccat tcattttcag
     2161 tttaccagta tctttatcta aatataaatg tttaccacca gtgccatcag aacccatttc
     2221 ataacttata atacctctgc cttctgaaac ttcaccgatg ataaccgcac ctgcaccatc
     2281 tccaaataga actgcagtag aacggtcagt taaatctgtt attttagata atttatctgc
     2341 accgacaact aaaatattat gataatctcc agattgaaca tattgtttag ctgtaatcat
     2401 tgaatacata aatccagaac atgctgcaag ttgatccata gaggcaactt tgcccgtccc
     2461 taaacgttct tgcaacatat ttgcgacagt tggaaatggc atatctccag ttgctgtggc
     2521 aacaattatc atatctatat cttcaggctg aataccagcg tcagcgattg cttttacact
     2581 tgcttcatat gctaaatctg aagtatcttg atcgtcatct gcccaatgtc tttctttaat
     2641 tccagtcatc ttagaaatcc attcatcaga tgtatctaaa aattgctcaa aataggcatt
     2701 gtcaataatc ttttctggtg catatgcacc aaaaccttta atacccacgt tcatagttga
     2761 acaccttctt aataaaattt ttaataccag gtattaattt aacatagatt actttttaaa
     2821 ctcaagtcag aggtaggaat attgactagt taataaaatt ttaatgcttt attatcgtca
     2881 aatttcagta tgaaattggt tttaatgcgc ttaaatttga atagccattc agataaaatc
     2941 atttatttaa actgtataat atggtcaaaa tatatgagat gttactatac aaatttctaa
     3001 ctttcactta aaataaaata tatataatta aagtggaggt agaatgacat gaaacacctc
     3061 gtaacattct tttgggcatt attattaatg caaatggtta actttgtact taatagccta
     3121 gtaggtggag acagcttaaa tgtagtgaat ccaattatca tggcagtatt gttcacgatt
     3181 ttcacagcaa tttttgctgc agtaattaaa ccgccgaaag attcatcaca ataactattt
     3241 agtataaaat gaaatatgtc tttcttatta ttgaagctgc ccagataaag gcggctttta
     3301 tttttggcca ttaatgtttt gtattttgaa tattttaaag ctcaataacc tctaacccat
     3361 cacttttaaa tttagaaaaa gccttaatac aaagatgtca tttacgagta atctt
//
LOCUS       DAHHSC010000220         3409 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.223, whole genome shotgun sequence.
ACCESSION   DAHHSC010000220 DAHHSC010000000
VERSION     DAHHSC010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3409)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3409)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3409
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.223"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            85..879
                     /locus_tag="NBI57_002373"
     CDS             85..879
                     /locus_tag="NBI57_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein"
                     /protein_id="HCD1823300.1"
                     /translation="MIHSKKLTLGICLVLLIILIVGYVIMTKTNGRNAQIKDTFNQTL
                     KLYPTKNLDDLYDKEGFRDQEFKKGDKGTWIVNSEMVIEPKGKDMETRGMVLYINRNT
                     RTTKGYYFISEMTDDSNGRPKDDEKRYPVKMEHNKIIPTKPLPNDKLRKEIENFKFFV
                     QYGDFKDINDYKDGDISYNPNVPSYSAKYQLKNDDYNVKQLRKRYNIPTNKAPKLLIK
                     GDGDLKGSSVGSKNLEFTFVENKEENIYFTDSVQYTSSEDTSYESN"
     gene            complement(883..1005)
                     /locus_tag="NBI57_002374"
     CDS             complement(883..1005)
                     /locus_tag="NBI57_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1823301.1"
                     /translation="MSYIIKFTFKFFEMATYISQLTLDIELVSSELLVILPGFI"
     gene            1440..1631
                     /locus_tag="NBI57_002375"
     CDS             1440..1631
                     /locus_tag="NBI57_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1823302.1"
                     /translation="MRKEIEALIFSDVSSYDIYVNTGVNQGLIGDIKDGYLTIDSMPY
                     IDAERLYHFAMERKSLVTN"
     gene            complement(1770..1955)
                     /locus_tag="NBI57_002376"
     CDS             complement(1770..1955)
                     /locus_tag="NBI57_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1823303.1"
                     /translation="MLYILITALNVILNKLKMKDSNIISFIYSRHDRILYVNLVGNRF
                     KNNISMTKATSNIGRGF"
     gene            complement(1891..2172)
                     /locus_tag="NBI57_002377"
     CDS             complement(1891..2172)
                     /locus_tag="NBI57_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HCD1823304.1"
                     /translation="MTYHERVLALRAESKRTAFDFRFEDLFSKEEWLSMSLAERQKAE
                     KAFRHEVKNMDDVRMPFSSVHDAQVKLYNVVYSYNGIKRNFKQVENEGF"
     gene            2461..3156
                     /locus_tag="NBI57_002378"
     CDS             2461..3156
                     /locus_tag="NBI57_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCD1823305.1"
                     /translation="MTVLTDKIAVVTGAGSGIGEAIATLLHEEGAKVVLAGRNKDKLQ
                     NVANQLAQDSVKVVPTDVTNKEEVDELMKIAQQTFGGLDIVINSAGQMLSSKITDYQV
                     DEWDSMIDVNIKGTLYTAQAALPTMLEQSSGHLINIASISGFEVTKSSTIYSATKAAV
                     HTITQGLEKELAKTGVKVTSISPGMVDTAITAAYNPSDRKKLDPQDIAEAVLYALTQP
                     KHVNVNEITVRPV"
BASE COUNT         1271 a          480 c          604 g         1054 t
ORIGIN      
        1 tcataaagtg ttcaaaataa tttttcgctt tagttgtata aattaaagat tgagtcaata
       61 ttaaaaatat aaggtggaga aatgatgatt cattcaaaga aattaacgct cggtatatgc
      121 ttggtattac tcattatatt gattgtaggt tatgtcatta tgacaaaaac aaatggtcga
      181 aacgcccaaa ttaaagacac atttaatcaa acattaaaat tatatccaac caaaaatctc
      241 gatgatttgt acgataaaga aggctttcga gatcaagaat ttaaaaaggg tgataaaggt
      301 acttggatag ttaattctga aatggtaatc gagccaaaag gtaaggatat ggaaacgaga
      361 ggaatggtgc tctatatcaa tcgcaatact agaaccacaa aagggtatta ttttataagt
      421 gaaatgacag atgacagtaa cggcagacca aaggatgatg aaaaaaggta tccggtaaaa
      481 atggaacata ataaaatcat accaacgaag ccactaccga atgacaagtt aagaaaagaa
      541 atcgaaaact ttaaattctt tgtacaatac ggtgacttta aagatattaa tgattataaa
      601 gatggtgaca tttcatataa tcctaatgtt ccaagttatt ccgcaaaata tcaattgaaa
      661 aacgatgact acaatgtgaa gcaacttcga aaaagatata atattccaac caacaaagct
      721 ccgaaattat taataaaagg tgatggtgat ttaaaaggct catctgtagg gtcaaaaaat
      781 ctagaattta cctttgtaga aaataaagaa gaaaacattt attttacaga ttctgtacaa
      841 tatacttcaa gtgaggatac aagttatgag tcaaactgaa tatcaaataa aacctggtaa
      901 tataacaagt aactctgaag aaacaagttc gatatctaaa gtgagctgtg aaatataggt
      961 agccatttca aaaaatttaa aggtgaattt gataatgtag ctcaaggaga ttgggttaaa
     1021 aaggcgaaga atgaactgga tgatattagt aagaaattaa aaaatattca aagaacggaa
     1081 gtttaatatt ctagccgatt cctgaaacta agacagcatg cgttcattca tgccattatt
     1141 aatataagca ccgcaacaaa aaagcttcta atgtgataca ggaacctcat attccgtatc
     1201 atgttagaag cttttaatgt ctaaagaaca tctacatttt atcatatttt ctgacttatt
     1261 aaacttttat ataattaact atttcttaat tttccaaaat agtgataaat ttgtgaaata
     1321 catcacaaac ctctttattt atttggaaat tcatgtaata ttagacttgt aagaagttaa
     1381 taaatagaga gagacgagag agtttatata aataatatat aaacattgga gtgatgatta
     1441 tgagaaaaga gattgaagcg cttattttct cagacgtaag tagctatgat atttacgtga
     1501 acactggtgt aaatcaagga ttaattggtg acatcaaaga tggttaccta actattgatt
     1561 ctatgcctta cattgatgct gagcgtttgt atcactttgc tatggaacgt aaatcgttag
     1621 tcactaacta gttcttattg ccaatgatta ctacccctag tcggcggcaa ttgaagtgtg
     1681 attgatgtaa cttgccctcg ttggtgagca attgagggca gatcccttta attaagtaaa
     1741 ccctaactcc ccacaaatct ggaacgatac taaaagccac gtcctatatt ggatgtggct
     1801 ttagtcatac ttatattatt tttaaaacga ttacctacaa gatttacata taaaattcta
     1861 tcatgtctgc tatatataaa cgaaatgata ttagaatcct tcattttcaa cttgtttaaa
     1921 attacgttta atgccgttat aagaatatac aacattatat aattttactt gggcgtcatg
     1981 gacacttgag aagggcattc ttacatcgtc catattttta acttcgtgtc gaaatgcttt
     2041 ttcagctttt tgtctttctg caagagacat acttagccat tcttctttgc taaataaatc
     2101 ttcgaatcga aaatcaaatg cggttctttt actttctgct cttaatgcta aaacacgctc
     2161 atgatatgtc acgtttaatt cctccctcta atcattcaat cgaatgataa atcacagtat
     2221 taactttagt actaatataa tgtttttaca ataaagaagt gacgattttt caacattttt
     2281 aaaaataaaa aatataagca acgattaatt gtatatttcg aatggcttgt aatgtaattt
     2341 atctatgaaa atgaagcgag ttattatgac cgttatatga attgtgatac gctttttaaa
     2401 agtaatatgt atttatgatt taccctgaaa aaataaaaag aatcaaagga gtcgatcatt
     2461 atgacagtat taacagataa aatagcagta gttacaggtg caggtagtgg tattggagaa
     2521 gcaattgcaa cattactaca tgaagaaggg gcgaaagttg tcttagccgg tagaaataaa
     2581 gataaactac aaaatgtggc gaatcaatta gctcaagata gtgtgaaggt agtgccaaca
     2641 gatgtaacga ataaagaaga agtcgatgaa ttgatgaaaa ttgcacaaca aacattcggt
     2701 ggtttggata ttgttatcaa tagtgcggga caaatgttat cgtctaagat tactgattat
     2761 caagtagatg agtgggatag tatgattgat gtgaatatca aaggcacttt atatacggca
     2821 caggctgcat taccaactat gttagaacaa tcaagtggac atcttattaa cattgcatct
     2881 atttctggct ttgaagtaac gaaaagtagt acgatttata gtgcgacgaa agcagcagtt
     2941 cacactatta ctcaaggatt agaaaaagag ttggcaaaga caggcgttaa agtaacaagc
     3001 atttctccag gaatggtaga tacagcaata acagccgcat ataatccaag tgatcgtaaa
     3061 aagcttgatc cacaagatat tgcagaagcg gtattgtatg cattaacaca accaaagcat
     3121 gttaacgtga atgaaattac agtgcgtcct gtataaaaag gtgtttgagg tgtgggagat
     3181 agacatttaa tgtaatagct atcgattttg ttaatgttag taaggcaata atgacatgag
     3241 ggcataatag gcaattattc cttgtacaat tctcagtaat tggtgatttt aacaatcaag
     3301 ttaatgaagg cgacgatgtt agtgttatgg tttataatct ataatgttcg taattacaga
     3361 ttacaaaaaa atttcatttt ccaccacatt ttaggagtta ctttaatga
//
LOCUS       DAHHSC010000221         3376 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.224, whole genome shotgun sequence.
ACCESSION   DAHHSC010000221 DAHHSC010000000
VERSION     DAHHSC010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3376)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3376)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3376
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.224"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            complement(34..2076)
                     /locus_tag="NBI57_002379"
     CDS             complement(34..2076)
                     /locus_tag="NBI57_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002465267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HCD1823306.1"
                     /translation="MEIFETILIFIAVVILSSFVHTFIPKVPLAFIQIFLGMLLFITP
                     IPVQFNFDSELFMVTMIAPLLFVEGVNVSRVHLRKYIKPVMMMALGLVITTVIGVGLF
                     IHWIWPDLPIGAAFAIAAILCPTDAVAVQAITKGKVLPKGAMTILEGESLLNDAAGII
                     SFKIAVGVLVTGAFSLVDAVQLFLIASIGGAVVGLLIGMALVRFRLTLMRRGYENINM
                     FTIIQLLTPFVTYLIAELFHASGIIAAVVAGLVHGFERDRIMQVRTQLQMSYNHTWNI
                     LGYVLNGFVFSILGFLVPEVIIKIIKTEPHNLIFLIGITIVVALAVYLFRFVWVYVLY
                     PYFYLAISPFQKMMTKNDDDNPTTEKPPKRSLYALIMTLCGVHGTISLAIALTLPYFL
                     AGHHAFTYRNDLLFIASGMVIISLVVAQVLLPLLTKPAPKTVIGNMSFKVARIYILEQ
                     VIDYLNQKSTFETSFKYGNVIKEYHDKLAFLKTVEKDDENSKELERLQKIAFNVETKT
                     LESLVDEGQITNSVLENYMRYAERTQVYRQASLIRRMIVLLRGALLKRRVQTRVNSAS
                     SLSVTDNLMELNKINKLVHYNVVSRLSKETTKDNTLEIGMVCDGYLMRIENLTPSNFF
                     NSASEDTITKIKLNALREQRRILRELIDTDEVSEGTALKLREAINYDEMVIVDSMT"
     gene            complement(2416..2853)
                     /gene="mnhG2"
                     /locus_tag="NBI57_002380"
     CDS             complement(2416..2853)
                     /gene="mnhG2"
                     /locus_tag="NBI57_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001566533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh2 subunit G"
                     /protein_id="HCD1823307.1"
                     /translation="MEITKEIFSLIAAVMLLLGSFIALISAIGIVKFQDVFLRSHAAT
                     KSSTLSVLLTLIGVLIYFIVNTGFFSVRLLLSLVFINLTSPVGMHLVARAAYRNGAYM
                     YRKNDAHTHASILLSSNEQNSTEALQLRAKKREEHRKKWYQND"
     gene            complement(2828..3130)
                     /gene="mnhF2"
                     /locus_tag="NBI57_002381"
     CDS             complement(2828..3130)
                     /gene="mnhF2"
                     /locus_tag="NBI57_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000616642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh2 subunit F"
                     /protein_id="HCD1823308.1"
                     /translation="MIQTITHIMIISSLIIFGIALIICLFRLIKGPTTADRVVTFDTT
                     SAVVMSIVGVLSVLMGTVSFLDSIMLIAIISFVSSVSISRFIGGGHVFNGNNKRNL"
     gene            complement(3127..>3376)
                     /gene="mnhE2"
                     /locus_tag="NBI57_002382"
     CDS             complement(3127..>3376)
                     /gene="mnhE2"
                     /locus_tag="NBI57_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh2 subunit E"
                     /protein_id="HCD1823309.1"
                     /translation="YILFKTKDMNPGLLSYETRLTSDWAITFLTILIIITPGSTVIRI
                     SQDSKKFFIHSIDVSEKEKDSLLRSIKHYEDLILEVSR"
BASE COUNT         1195 a          600 c          458 g         1123 t
ORIGIN      
        1 tttttcatca atccctttta gcataattag gaactacgtc atactatcta caataaccat
       61 ttcatcataa ttgatggctt ctcttagttt taacgctgta ccttctgata cttcatctgt
      121 atcaatcaac tcacgtaaaa tgcgacgttg ttctctcaat gcatttaatt taattttagt
      181 aatcgtatct tcacttgctg agttgaagaa atttgatggt gttaagtttt caattcgcat
      241 taaataaccg tcacaaacca ttccaatttc aagtgtatta tcttttgttg tttccttaga
      301 caaacgacta accacattat aatggactaa tttattaatt ttatttaatt ccattaagtt
      361 atccgtaaca ctaagtgaag atgcggagtt cactctcgtt tgtactcttc gttttaataa
      421 agcacctcgt aataatacaa tcattcttct tattaatgat gcttgtctat atacctgtgt
      481 tctttcagca taacgcatat agttttcaag tacactattc gttatttgtc cttcatctac
      541 taaagactct aatgtttttg tttctacatt aaaagcaatt ttttgtagac gttctaattc
      601 tttagagttt tcatcatctt tctctacagt ttttaaaaat gctaatttat catgatattc
      661 tttaatcacg ttaccatatt taaaacttgt ttcgaaagta gatttttgat ttagataatc
      721 aataacttgt tctaatatat aaattctagc aactttaaac gacatattgc caattactgt
      781 tttaggtgca ggtttcgtta ataatggcaa taatacttgc gcaactacca aactaataat
      841 aaccatacca gatgcaataa ataataagtc gtttctatac gtaaaagcat gatgccctgc
      901 taaaaaataa ggtaacgtta atgcgattgc taaagaaatt gttccatgca caccacataa
      961 cgtcataatt aaagcgtata aacttcgctt tggtggtttc tcagtcgttg gattatcatc
     1021 atcattttta gtcatcattt tttggaatgg actgatggct aaataaaaat aaggatataa
     1081 gacataaacc caaacaaatc taaatagata gacagctaaa gcaacaacaa tagtgatgcc
     1141 tattaaaaag attaaattgt gcggttctgt tttgataatt ttaataataa cttcaggtac
     1201 taaaaatcct aatattgaaa aaacaaagcc atttaaaaca taacctagta tattccatgt
     1261 atgattgtaa ctcatttgca gttgtgtacg tacttgcata attctgtcac gttcgaaacc
     1321 atgtacaagt cctgcaacta ctgctgcaat gattcctgat gcgtgaaaca attcagcaat
     1381 taaatacgta acaaatggtg ttaacaattg aataattgta aacatattaa tgttttcata
     1441 tcctcgacgc atcaatgtta atcggaacct tactaatgcc atacctataa gtaaaccaac
     1501 cactgcgcca ccaattgatg caattaaaaa caactgaaca gcatcaacaa gtgaaaaagc
     1561 acctgtaact aatactccaa cagctatttt aaatgaaata ataccagcag catcattcaa
     1621 taatgactca ccttcaagaa ttgtcattgc tccttttggc aagacctttc ctttagtgat
     1681 tgcttgcact gctactgcat cagtaggaca aagaatggca gcaattgcaa atgctgctcc
     1741 aataggtaaa tctggccaaa tccaatgaat aaataaacct acacctatca cagtagtaat
     1801 gactaatcct aatgccatca tcatcactgg cttaatatat ttccttaaat ggactctaga
     1861 aacattaaca ccttctacaa ataacaaagg cgcaatcatt gttaccataa acaattcaga
     1921 atcaaaatta aattgaacag ggattggggt aataaatagt aacatgccca agaaaatttg
     1981 tataaatgct aggggtactt taggtatgaa agtatggaca aacgaactta gtatcacaac
     2041 agctataaat ataagaattg tttcaaatat ttccaaactt tcacctctct aaaaagtatt
     2101 gtttaattga aaattaagta tcacatctcg ttgtaattat actttagagg ataaattgag
     2161 ttagcgacca caaaagcact ttaatataga tatatgtcta cgattgcagt acttaaattt
     2221 gcaattattt aattttattt tatcactaat tgtttgtata aataaacaac ttgctttcac
     2281 ataacaacat taacttataa tacaaaaaat gagcacctta aaatcgacta accaatttca
     2341 aagtactctt ttaatgatta attttgaaaa cagattttca aaagcattgt tatgcttaac
     2401 aatttagcca acacttcaat cgttttgata ccatttctta cgatgctctt ctcgtttttt
     2461 agcacgtaat tgtaatgctt ctgtagagtt ttgttcattt gaacttaata atattgatgc
     2521 atgtgtgtga gcatcatttt ttcgatacat ataagcgccg ttgcgataag cagcgcgagc
     2581 gactaagtgc atgccgactg gtgaagttaa attaataaaa acaagtgaca gtaataaacg
     2641 cacactgaaa aatcctgtat tcacaataaa ataaattaat acaccgatta aagttaataa
     2701 cacggataaa gttgaacttt ttgtcgcagc gtgacttctt aagaaaacat cttggaattt
     2761 cacgatacct attgcactaa taagagcaat aaaactacct aacaacaaca tcacagcagc
     2821 aataagacta aagatttctt ttgttatttc cattaaacac atgcccccca ccaataaagc
     2881 gtgatattga aacagaactt acaaaagata taatggcaat gagcatgatt gaatctaaga
     2941 aagaaacggt gcccataagt acacttaaca cacccacaat tgacattacg acagcacttg
     3001 ttgtatcaaa tgtaacgaca cgatctgctg ttgtaggtcc cttgattaat ctaaataaac
     3061 agatgattaa tgcaattcca aaaataatga gtgaactaat aatcataata tgtgttattg
     3121 tttgtatcat cgcgacacct ccaatattaa gtcttcataa tgcttaatac ttcttaacaa
     3181 actatctttt tctttttctg acacgtcgat actatgaata aaaaactttt tagagtcttg
     3241 agaaattcgt attactgtag accctggagt tataataatt aaaattgtta aaaatgttat
     3301 tgcccaatca cttgttagtc ttgtttcata tgaaagtaat ccagggttca tatcttttgt
     3361 tttaaaaaga atataa
//
LOCUS       DAHHSC010000222         3374 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.225, whole genome shotgun sequence.
ACCESSION   DAHHSC010000222 DAHHSC010000000
VERSION     DAHHSC010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3374
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.225"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            121..1422
                     /locus_tag="NBI57_002383"
     CDS             121..1422
                     /locus_tag="NBI57_002383"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HCD1823310.1"
                     /translation="MTATWEKKEGNEGLLTVTVPAEKVNKALDQAFKKVVKQINVPGF
                     RKGKVPRPIFEQRFGVEALYQDAIDILLPDAYGEAIDETDIKPVAQPEVSVTQIEKGK
                     DFIFEATVTVEPEVKLGDYKGLEIEKQETELSDDELQEAIDHSLGHLAEMVVKEDGVV
                     ENGDTVNIDFSGSVDGEEFEGGQAEGYDLEIGSGSFIPGFEEQLEGMKVDEEKDVVVT
                     FPEEYHAEELAGKEATFKTKVNEIKFKEVPELTDEIANELDAEANTVDEYKENLRKRL
                     AEQKATDAENVEKEEAITKATDNTTIDIPEAMVNTELDRMVSEFAQRIQQQGLDLQTY
                     FQISGQDETQLREQMKDDAEQRVKTNLTLTAIAEAEKIEATDEDIDKELEKMSKQFNI
                     SVEDIKNTLGNTDIIKNDVRIQKVIDLLRDNAKFVEGTKED"
     gene            1573..2835
                     /gene="clpX"
                     /locus_tag="NBI57_002384"
     CDS             1573..2835
                     /gene="clpX"
                     /locus_tag="NBI57_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001830765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HCD1823311.1"
                     /translation="MFKFNEDEENLKCSFCGKDQDQVKKLVAGSGVYICNECIELCSE
                     IVEEELAQNTSEAMTELPTPKEIMDHLNEYVIGQEKAKKSLAVAVYNHYKRIQQLGPK
                     EDDVELQKSNIALIGPTGSGKTLLAQTLAKTLNVPFAIADATSLTEAGYVGDDVENIL
                     LRLIQAADFDIDKAEKGIIYVDEIDKIARKSENTSITRDVSGEGVQQALLKILEGTTA
                     SVPPQGGRKHPNQEMIQIDTTNILFILGGAFDGIEEVIKRRLGEKVIGFSSNEADKYD
                     EQALLAQIRPEDLQAYGLIPEFIGRVPIVANLETLDVTALKNILTQPKNALVKQYTKM
                     LELDDVDLEFTEEALSAISEKAIERKTGARGLRSIIEESLIDIMFDVPSNENVTKVVI
                     TAQTINEETEPELYDAEGNLINNSKTSA"
     gene            2989..>3374
                     /locus_tag="NBI57_002385"
     CDS             2989..>3374
                     /locus_tag="NBI57_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HCD1823312.1"
                     /translation="MKVNPNNIELIISAVKEEQYPETELSEVALSGRSNVGKSTFINS
                     MIGRKNMARTSQQPGKTQTLNFYNIDEQLIFVDVPGYGYAKVSKTQREKFGKMIEEYI
                     TKRENLQLVIQLVDLRHDPTQDDILM"
BASE COUNT         1328 a          446 c          656 g          944 t
ORIGIN      
        1 gaaaaaagtt gatttacaaa tatataaacg ttgtgatttc aatgtttgta tagggaatag
       61 acctatgata taatagggaa gttgaaaaaa taaattagtt aatcatggag gtatatatac
      121 atgacagcaa cttgggaaaa aaaggaaggt aacgaaggtt tattaactgt tactgttcct
      181 gcagaaaaag taaacaaagc gttagatcaa gcattcaaaa aagtggttaa acaaattaac
      241 gtacctggat tccgtaaagg taaagtgcca cgtccaattt ttgaacaacg ctttggtgta
      301 gaagcattat atcaagatgc tatcgacatt ttattaccag atgcttatgg tgaagcaatt
      361 gacgaaactg atattaaacc agttgcacaa ccagaagtaa gtgttactca aattgaaaaa
      421 ggtaaagatt tcatttttga agcaacagtt acagttgagc cagaagttaa attaggagac
      481 tataaaggtc ttgaaattga aaaacaagaa actgaattat ctgatgatga gttacaagaa
      541 gcgattgacc acagcttagg acatttagct gaaatggtag ttaaagaaga tggtgttgtt
      601 gaaaatggcg acacagttaa cattgacttt agtggttcag ttgacggaga agaattcgaa
      661 ggtggacaag ctgaaggtta cgatttagaa atcggttcag gttcattcat acctggtttc
      721 gaagagcaat tagaaggtat gaaagttgac gaagaaaaag atgttgtcgt aacattccca
      781 gaagaatacc atgctgaaga attagctggt aaagaagcaa ctttcaaaac aaaagttaac
      841 gaaattaaat ttaaagaagt tccagaatta acagatgaaa ttgctaatga attagatgca
      901 gaagcaaata cagtagacga gtacaaagaa aacttacgta aacgtttagc tgaacaaaaa
      961 gctacagatg ctgaaaatgt tgaaaaagaa gaagcgatta caaaagctac tgataataca
     1021 acaatcgata ttcctgaagc aatggttaat actgaattag atcgtatggt gtctgaattt
     1081 gcacaaagaa ttcaacaaca aggtttagat ttacaaacgt acttccaaat ctcaggtcaa
     1141 gatgaaactc aattaagaga gcaaatgaaa gacgatgcag aacaacgtgt taaaactaac
     1201 ttaactttaa ctgcgatcgc tgaagctgaa aaaatcgaag ctactgatga agatatcgat
     1261 aaagaattag aaaaaatgag taaacaattt aatatctcag ttgaagatat caaaaatact
     1321 ttaggtaata ctgatatcat taaaaatgat gttcgtatcc aaaaagttat cgatttatta
     1381 agagataacg caaagttcgt tgaaggaact aaagaagatt aatcttcatt aaatattaaa
     1441 ttacaaaaat gagtagcaga tgcatagctt atgtatctgc tactattctt taagcaaaaa
     1501 gtttgtatgt taatatgttg cattgtaata tccaatctag tatagtcttt aacgaatagg
     1561 ggtgtaaaaa gaatgtttaa attcaatgaa gatgaagaaa atttgaaatg ctctttctgc
     1621 ggaaaagacc aagatcaagt aaaaaaactt gtagcaggaa gtggtgtata tatttgtaat
     1681 gagtgtattg aattatgctc agaaatcgtc gaagaagaat tagctcaaaa cacttctgaa
     1741 gcgatgacag aattacctac tcctaaagaa attatggatc atttaaacga atatgttatt
     1801 ggtcaagaaa aagctaaaaa atctttagct gtagctgttt ataaccacta taagcgtatt
     1861 caacaattag gaccaaaaga agatgatgtt gaattacaaa aaagtaacat tgcattaatt
     1921 gggccaacag gtagtggtaa aacattatta gctcaaacat tagccaagac gttgaatgta
     1981 ccatttgcaa ttgcagatgc gacaagttta actgaagctg gttatgtagg cgatgatgtt
     2041 gaaaatatct tgttgagatt aattcaagca gctgactttg acattgataa agccgaaaaa
     2101 ggtattattt atgtagatga aattgataaa attgcacgta aatctgaaaa cacatctata
     2161 acacgtgacg tttcaggtga aggtgttcaa caagcattgc ttaaaatctt agaaggtacg
     2221 actgcaagtg ttccgccaca aggtggacgc aaacatccaa accaagaaat gattcaaatt
     2281 gatacaacaa atatcttatt tattcttggt ggtgcctttg atggtattga agaagtgatt
     2341 aagcgccgtc ttggtgaaaa agttattggt ttctcaagca atgaagctga taaatatgac
     2401 gaacaagcat tattagcaca aattcgccca gaagatttgc aagcctatgg tttgattcct
     2461 gaatttatcg gacgtgtgcc aattgtagct aatttagaaa cattagatgt aactgcgttg
     2521 aaaaacatct taacgcaacc taaaaatgca cttgtgaaac aatatactaa aatgctggaa
     2581 ttagatgatg tggatttaga gttcactgaa gaagctttat cagcaattag tgaaaaagca
     2641 attgaaagaa aaacaggtgc gcgtggttta cgttcaatca tagaagaatc gttaatcgat
     2701 attatgtttg atgtgccttc taacgaaaat gtaacgaagg tagttattac agcacaaaca
     2761 attaatgaag aaactgaacc agaactatac gacgcagaag gcaatttaat taataatagt
     2821 aaaacatcag cttaatcatt gatgtgttat aaaagtgaaa agctccaatc aaagttgacg
     2881 ctcaaatagt ttaactttga tcggagcttt tttataatgt ataccgaaag gttcaaatat
     2941 tatataatgg tagagttgaa agagaatata aaaaagtgag gatataaaat gaaagttaat
     3001 cctaataata ttgaattaat cattagtgca gtaaaagaag aacaatatcc agaaacagaa
     3061 ttgtctgaag ttgcactgag cggtcgatct aatgtaggta agtctacatt tatcaatagt
     3121 atgattggca gaaaaaatat ggcacgtaca tcacagcaac ccggcaaaac gcaaacgtta
     3181 aatttttata atatagatga acaacttatt tttgtggatg ttccagggta tggatatgct
     3241 aaagtaagta aaacacaacg tgaaaaattt gggaaaatga ttgaggaata tataactaag
     3301 agagagaatt tgcaattagt tattcaatta gttgatttaa gacatgatcc aacacaagat
     3361 gatatcttaa tgta
//
LOCUS       DAHHSC010000223         3370 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.226, whole genome shotgun sequence.
ACCESSION   DAHHSC010000223 DAHHSC010000000
VERSION     DAHHSC010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3370)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3370)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3370
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.226"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            complement(239..1075)
                     /locus_tag="NBI57_002386"
     CDS             complement(239..1075)
                     /locus_tag="NBI57_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014532577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HCD1823313.1"
                     /translation="MDLASKYFNGVNWRYIDHSSGLEPMQSFAFDDTFCESVGKDISD
                     NVVRTWIHQHTVILGIHDSRLPFLKDGIDYLTNEIGYNAIVRNSGGLGVVLDQGVLNI
                     SLMFKGQTETTIDEAFTVMYLLISKMFEDENVDIDTMEIEHSYCPGKFDLSIDGKKFA
                     GISQRRVRGGIAVQIYLCVEGSGSERALMMQTFYEHALKGEVTKFKYPEIEPSCMASL
                     ETLLNKTITVQDVMFLLLYAIKDLGGVLNMTPITQEEWQRYDTYFDKMIERNKKMIDQ
                     MQ"
     gene            complement(1078..2064)
                     /gene="pta"
                     /locus_tag="NBI57_002387"
     CDS             complement(1078..2064)
                     /gene="pta"
                     /locus_tag="NBI57_002387"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HCD1823314.1"
                     /translation="MADLLNVLKDKLSGKNVKIVLPEGEDERVLTAATQLQATDYVTP
                     IVLGDETKVQSLAQKLDLDISNIELINPATSELKAELVQSFVERRKGKATEEQAQELL
                     NNVNYFGTMLVYAGKADGLVSGAAHSTGDTVRPALQIIKTKPGVSRTSGIFFMIKGDE
                     QYIFGDCAINPELDSQGLAEIAVESAKSALSFGMDPKVAMLSFSTKGSAKSDDVTKVQ
                     EAVKLAQQKAEEEKLEAIIDGEFQFDAAIVPGVAEKKAPGAKLQGDANVFVFPSLEAG
                     NIGYKIAQRLGGYDAVGPVLQGLNSPVNDLSRGCSIEDVYNLSIITAAQALQ"
     gene            2232..2984
                     /locus_tag="NBI57_002388"
     CDS             2232..2984
                     /locus_tag="NBI57_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-dependent peroxidase"
                     /protein_id="HCD1823315.1"
                     /translation="MSQAAETLDGWYSLHLFYAVDWASLRIVPKDERDALVTEFQSFL
                     ENTATVRSSKSGDQAIYNITGQKADLLLWFLRPEMKSLNHIENEFNKLRIADFLIPTY
                     SYVSVIELSNYLAGKSDEDPYENPHIKARLYPELPHSDYICFYPMNKRRNETYNWYML
                     TMEERQKLMYDHGMIGRKYAGKIKQFITGSVGFDDFEWGVTLFSDDVLQFKKIVYEMR
                     FDETTARYGEFGSFFVGHLINTNEFDQFFAIS"
BASE COUNT         1073 a          614 c          495 g         1188 t
ORIGIN      
        1 tcaatagaag gtcgtttctt tcatagtcat acgcattata tataccctat tctcaatcta
       61 tttaatacgt aaaacatgaa attttcttat taaatttatt atttccatca tatcattact
      121 tttaatttaa tgatgttcaa tttaaatatt aggtcaataa catatttatg ctttttatgg
      181 atactttcaa aaataacagc cccaaacgat aacttgaaag gggctgttaa atatttaact
      241 attgcatttg gtctatcatt ttcttgtttc tttcaatcat tttatcaaaa tacgtatcgt
      301 atctttgcca ttcttcttga gtaatgggcg tcatatttaa tacaccgcca agatctttga
      361 ttgcatataa tagtaaaaac attacatctt gaacagtaat cgttttgtta agcaatgtct
      421 ctaatgaggc catacaagat ggttcaattt caggatattt aaatttagtc acttcacctt
      481 ttaaagcatg ttcataaaat gtttgcatca tcaatgcacg ttctgaacca gagccttcaa
      541 cacaaagata aatttgtaca gcaataccgc ctctaactct tcgttgcgat atgcctgcaa
      601 atttcttacc atcgatactt aagtcaaatt ttcctgggca ataagaatgt tcaatttcca
      661 tcgtatcaat atcaacattc tcatcttcga acattttgct aattaagagg tacatcacag
      721 taaacgcttc atcaatcgtt gtttctgttt gtcctttgaa catcagcgat atatttaata
      781 caccttgatc tagaacgaca cctaagccac cagaatttct aacaatggca ttataaccaa
      841 tctcattcgt taaataatca atgccatctt ttaaaaacgg caatcttgaa tcatgaatac
      901 caagaataac agtatgttga tgaatccaag tacgcacaac attatctgat atatctttgc
      961 ccacactttc gcaaaatgta tcatcgaatg cgaaagattg cataggttct aatccagaag
     1021 aatggtcgat atatcgccag ttgacgccat taaaatattt actcgctaaa tccatcgtta
     1081 ttgtaaggct tgcgctgctg taataattga aagattgtat acatcttcaa ttgagcagcc
     1141 acgtgataag tcatttactg gagaatttaa accttgtaat actggaccaa ctgcatcata
     1201 tccacctaaa cgttgtgcaa ttttgtaacc aatattacca gcttctaaac ttgggaatac
     1261 aaagacattt gcatcacctt gtaatttagc acctggcgct tttttctcag caacacctgg
     1321 tacaatcgca gcatcaaatt ggaattcgcc atcaatgatt gcttctaatt tttcttcttc
     1381 agctttttgt tgtgctaatt tgacagcttc ttgaactttt gtcacgtcgt ctgatttagc
     1441 agaccctttt gttgaaaagc ttaacattgc aacttttgga tccatgccaa agcttaatgc
     1501 tgattttgca ctttctactg caatttctgc aagtccttgt gaatcaagtt ctggattgat
     1561 tgcacaatca ccaaagatgt attgttcatc acctttaatc ataaagaaga tacctgatgt
     1621 tcttgataca cctggtttcg ttttgatgat ttgtaaagct ggacgcacag tgtctcctgt
     1681 tgaatgtgct gcaccactaa ctaaaccatc tgctttacca gcataaacaa gcattgtacc
     1741 gaagtagttc acattgttta ataattcttg tgcttgttct tcagtcgctt tacctttacg
     1801 tcgttcaaca aatgattgaa ctaattcagc tttcaattca cttgtcgcag gattaattaa
     1861 ttcaatatta gaaatatcaa gatcaagttt ttgcgctaaa gattgaacct tagtctcatc
     1921 acctaacacg attggtgtaa cataatctgt tgcttgtaat tgtgttgcag ctgttagaac
     1981 acgttcgtcc tctccttcag gtaatacgat tttaacgttt ttaccagaaa gtttgtcttt
     2041 taatacattt aataaatcag ccataatgtc ctcctgtaat ataaatctta ttaatcattc
     2101 acggtataat tatacgccat tttatttcat aatgccacgt caccaatttg taatcataat
     2161 tttatcgtta cccctttgct ttatgataaa atttataaag aactgatgat ttttgaaaag
     2221 gagcgataaa catgagtcaa gcagccgaaa cattagatgg ttggtatagt ctacatttat
     2281 tttatgcagt tgattgggca tcattacgta tagttccaaa ggacgaacgc gatgcacttg
     2341 tcactgaatt tcaatcattt ttagaaaata cagcaactgt aagatcatca aaatctggtg
     2401 atcaagctat ttataatata actggtcaaa aagcagattt gttattatgg ttcttacgtc
     2461 ctgaaatgaa gtctttaaat catattgaaa atgaatttaa caaattgcgc attgctgact
     2521 tcctaatccc tacatattca tatgtatcag tcattgaatt gagcaattat ttagctggta
     2581 aatctgatga agatccttat gagaaccctc atatcaaagc aagattatac ccagaattac
     2641 cacattctga ttatatttgt ttctatccaa tgaacaaacg tcgtaatgaa acttataact
     2701 ggtacatgtt aactatggaa gaacgccaaa aattaatgta tgaccatggt atgattggta
     2761 gaaaatatgc tggcaaaatc aaacaattta ttactggttc tgtagggttt gatgatttcg
     2821 aatggggcgt aacattgttc tcagatgacg tattacaatt caaaaaaatt gtatacgaaa
     2881 tgcgctttga tgaaacaaca gcacgatacg gtgaattcgg tagtttcttt gtaggacatc
     2941 ttattaacac aaacgaattc gatcaattct ttgcgatttc ttaatacatt ggtacgttta
     3001 taaattaata aaaaaattcc aagcttatcg gtttaagctt ggaatttttc gtttatcttc
     3061 agtatattcc cgtatacata agacgtgatt tggtaaatag ttgaaatctg tatgtttaaa
     3121 cttatatata tgtgctaatg tattatcaat aacaaagtac actttgctca tagcaaagtg
     3181 tacccgagta gtcttccttg ggagaacttt aactactatc actacatata aacgttaacc
     3241 tcaatagaaa ttatacagtc gctactctat acaatttttg taatggttaa ctaatattat
     3301 tttaacctat ttgaaatatt tgaaacatat ttttgtcgaa tttttttcaa taattttccc
     3361 tttttatact
//
LOCUS       DAHHSC010000224         3346 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.227, whole genome shotgun sequence.
ACCESSION   DAHHSC010000224 DAHHSC010000000
VERSION     DAHHSC010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3346)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3346)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3346
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.227"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            <1..218
                     /locus_tag="NBI57_002389"
     CDS             <1..218
                     /locus_tag="NBI57_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HCD1823316.1"
                     /translation="MISGVNIPGYFSTIAAILLLGGIQLISIGVVGEYIGRIYYEVKA
                     RPKYIIQATNLSSIENDEKDTHKVYSK"
     gene            complement(290..1345)
                     /gene="saeS"
                     /locus_tag="NBI57_002390"
     CDS             complement(290..1345)
                     /gene="saeS"
                     /locus_tag="NBI57_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     SaeS"
                     /protein_id="HCD1823317.1"
                     /translation="MVLSIRSQIIIGVVSSILLTSTILAIAYILMWFNGHMTLTLTLT
                     TIITSCLTLLICSIFINPLIQKIKQFNIKTKQFANGNYASNDKTFNSPKEIYELNQSF
                     NKMASEITQQMNQIKSEQQEKTELIQNLAHDLKTPLASIISYSEGLRDGIITKDHEIK
                     ESYDILIKQANRLSTLFDDMTHIITLNTGKTYPPELIQLDQLLVSILQPYEQRIKHEN
                     RTLEVNFCSEIDAFYQYRTPLERILTNLLDNALKFSNVGSRIDINISENKDQDTIDIA
                     ISDEGIGIIPELQERIFERTFRVENSRNTKTGGSGLGLYIANELAQQNNAKISVSSDI
                     DVGTTMTVTLHKLDITS"
     gene            complement(1345..2031)
                     /locus_tag="NBI57_002391"
     CDS             complement(1345..2031)
                     /locus_tag="NBI57_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCD1823318.1"
                     /translation="MTHLLIVDDEQDIVDICQTYFEYEGYKVTTTTSGKEAISLLSND
                     IDIMVLDIMMPEVNGYDIVKEMKRQKLDIPFIYLTAKTQEHDTIYALTLGADDYVKKP
                     FSPRELVLRINNLLTRMKKYHHQPVEQLSFDELTLINLSKVVTVNGHEVPMRIKEFEL
                     LWYLASRENEVISKSELLEKVWGYDYYEDANTVNVHIHRIREKLEKESFTTYTITTVW
                     GLGYKFERSR"
     gene            complement(2006..2479)
                     /locus_tag="NBI57_002392"
     CDS             complement(2006..2479)
                     /locus_tag="NBI57_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HCD1823319.1"
                     /translation="MNKLLLLVTFIIRVGSGIVMLMQGYEKLTGGFTLKGLVPVIANN
                     TDSPEWYKWFFANIVAHTTSLFDIVVPLGEIAIGLGLIFGVFAYAASFFGAFVMINYI
                     LADMIFTYPLQLTFFILLLMSHSLLKQISLKEIINYFRGRKNRGEKIDDPLTDRG"
     gene            complement(2822..3262)
                     /locus_tag="NBI57_002393"
     CDS             complement(2822..3262)
                     /locus_tag="NBI57_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DM13 domain-containing protein"
                     /protein_id="HCD1823320.1"
                     /translation="MNTKYFLAVGAVASVLTLGACGNSNSQDQGNKTEQKTKSEDSNV
                     KTDKTKHLTGTFSSKNGETVEGKAEIKNGKLMLTNYKSSKGPDLYVYLTKNGDIKNGK
                     EIAMVDYDKEKQTFDLKNVDLSKYDEVTIYCKKAHVIFGGAKLK"
BASE COUNT         1042 a          513 c          526 g         1265 t
ORIGIN      
        1 ttatgatatc tggtgttaat attccaggat atttttcaac gattgcagct attttattat
       61 taggcggcat acagttaatt tcaattggtg ttgtaggtga atatattggc aggatatatt
      121 atgaagttaa ggcacgtcct aaatatatta ttcaagctac aaatctttca agtattgaaa
      181 atgatgagaa ggatacccat aaagtttatt ctaaataaac aaaaaaagaa gccctcatta
      241 atgggagctt ctttttagtc tttgcatttt attttataaa taaatcggat tatgacgtaa
      301 tgtctaattt gtgtaatgtt acagtcatcg tagttcctac atctatatca ctgcttacac
      361 tgatttttgc gttattttgt tgcgcgagtt cattagctat atataagcct aatccagaac
      421 cacccgtttt tgtattacga gagttttcta ctctgaatgt acgttcgaat atacgttctt
      481 gtagttctgg tataatgcca ataccttcat cgctaatagc aatgtcgata gtatcttgat
      541 ctttgttttc actaatatta atatcaatgc gactaccaac atttgaaaat tttagcgcat
      601 tatcaagtaa gtttgttaaa atacgctcaa gtggcgttcg atattgataa aatgcatcaa
      661 tttcgctaca gaaattcact tctaatgtgc ggttttcatg tttgatacgt tgctcatatg
      721 gttgcaatat tgatacaagt aattggtcta gttgtattaa ttctggggga tatgttttac
      781 ctgtatttaa agtgataata tgagtcatat catcaaataa tgttgataat ctgtttgctt
      841 gtttaattaa tatgtcgtat gactctttaa tctcatgatc ctttgtgatt ataccatcac
      901 gtagtccttc agaatatgaa ataatgcttg ctaaaggtgt ttttaaatca tgggctaagt
      961 tttgaatcag ttctgttttt tcttgttgtt cggatttaat ttgattcatt tgttgcgtaa
     1021 tttcagaagc cattttatta aaagattgat ttaattcata aatttctttt ggtgaattaa
     1081 acgttttatc attgcttgcg taatttccgt tagcaaattg cttagttttt atattaaact
     1141 gcttaatttt ttgtataagt ggattaataa aaatactaca tattaataag gttaaacagc
     1201 ttgtaattat tgtcgttaag gtcaaagtta gtgtcatatg gccgttaaac cacattaaaa
     1261 tatatgcaat tgctaaaata gttgaagtta atagtatact cgatacgacg ccaataatga
     1321 tttgacttct aattgataac accattatcg gctcctttca aatttatatc ctaatcccca
     1381 tacagttgtg atggtatatg ttgtaaagct ctctttttct aatttttctc taatacggtg
     1441 tatatggaca ttcacggtat tagcatcttc gtaatagtca tatccccaaa ctttttcaag
     1501 taattctgat ttagaaataa cttcattttc tctagaagct aaataccaca ataactcaaa
     1561 ttccttaata cgcataggga cttcgtgacc atttacagtc acaactttac ttaagttaat
     1621 aagtgttaat tcatcaaacg acagttgttc aactggttga tgatggtatt tcttcattct
     1681 tgtaagtaaa ttattaatac gtaaaacgag ttcccttgga ctaaatggtt ttttgacata
     1741 gtcatctgca cctaaagtta aggcgtaaat ggtatcatgt tcttgtgttt tggcagttaa
     1801 atagataaag gggatatcta atttttgcct tttcatttct ttgacaatgt cgtaaccatt
     1861 aacttctggc atcatgatat caagtaccat gatatcaata tcatttgata gtaaagaaat
     1921 tgcttcttta ccgctagttg tcgttgttac tttgtaacct tcatattcaa aataggtttg
     1981 acaaatgtct acaatgtctt gttcatcatc cacgatcagt aagtgggtca tctatttttt
     2041 cacctctgtt cttacgacct ctaaagtaat taatgatttc tttaagtgaa atctgtttta
     2101 acaatgagtg actcattagt aaaaggataa agaaagttaa ttgaagagga tacgtaaata
     2161 tcatatctgc taagatataa tttatcataa caaaggctcc aaagaaacta gcagcatatg
     2221 caaaaactcc aaaaattaaa cctaatccaa ttgcaatctc tccgagtggg acaacaatat
     2281 caaataatga cgtcgtatgt gcaactatat ttgcgaaaaa ccacttatac cactctggtg
     2341 aatcagtatt gttagcgatg actggtacta aacctttcag cgtaaatccg cccgttaatt
     2401 tttcgtagcc ttgcattaac ataacaatac ctgaacccac acgaatgata aatgtaacga
     2461 gtagcaataa tttattcatg atgttcacat tctttctatt tattgtgtgt aatttatata
     2521 aacataagat taagcaacat aatgcgattt gtagtgttat gtgaacagga agtgtttgag
     2581 ctcatgggtt gcttaagcta aacaaacctc aagctctaaa aaaattttac ttatgtgctc
     2641 tacaatctta tgggatttaa tagttgtata tatattgaaa aaaggaaagt atgatttcta
     2701 caagctaggg gggctgtgaa atcatacgct ttccattgag taatgagtgt aatgtatatt
     2761 tttaaaattt gtaatgtata gtaatagtat gttgtatata aaagtttaac tcagaaattg
     2821 attattttaa tttagcgccg ccgaagatga cgtgagcttt tttacaatag attgttactt
     2881 catcatattt gcttaaatct acatttttaa gatcaaatgt ttgtttttct ttatcgtagt
     2941 caaccattgc gatttcttta ccgtttttaa tgtcgccatt ttttgttagg tagacgtata
     3001 aatctggacc ttttgatgat ttgtagttag taagcattaa tttaccattt ttaatctcag
     3061 ctttaccttc aacagtttca ccgtttttag aactgaatgt acctgttagg tgttttgttt
     3121 tatcagtttt aacattgcta tcttctgact ttgttttttg ttcagttttg ttaccttgat
     3181 cttgtgaatt agaattacca caagcgccta aagttaatac agaggcaaca gcaccaactg
     3241 ctaaaaaata ttttgtattc atgctaactc ctcatttctt caatttgata agttaagttt
     3301 aaaatgaagg caaataatat gccattaact aattcttaac ttcgtt
//
LOCUS       DAHHSC010000225         3238 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.228, whole genome shotgun sequence.
ACCESSION   DAHHSC010000225 DAHHSC010000000
VERSION     DAHHSC010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3238)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3238)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3238
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.228"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            175..1350
                     /locus_tag="NBI57_002394"
     CDS             175..1350
                     /locus_tag="NBI57_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diglucosyl diacylglycerol synthase"
                     /protein_id="HCD1823321.1"
                     /translation="MVTQNKKILIITGSFGNGHMQVTQSIVNQLNDMNLDHLSVIEHD
                     LFMEAHPILTSICKKWYINSFKYFRNMYKGFYYSRPDKLDKCFYKYYGLNKLINLLIK
                     EKPDLILLTFPTPVMSVLTEQFNINIPVATVMTDYRLHKNWITPYSTRYYVATKETKQ
                     DFIDVGIDPSTVKVTGIPIDNKFETPINQKQWLIDNNLDPDKQTILMSAGAFGVSKGF
                     DTMITDILAKSANAQVVMICGKSKELKRSLIAKFKSNENVLILGYTKHMNEWMASSQL
                     MITKPGGITITEGFARCIPMIFLNPAPGQELENALYFEEKGFGKIADTPEEAIKIVAS
                     LTNGNEQLTNMISTMEQDKIKYATQTICRDLLDLIGHSSQPQEIYGKVPLYARFFVK"
     gene            1328..2518
                     /locus_tag="NBI57_002395"
     CDS             1328..2518
                     /locus_tag="NBI57_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001154225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCD1823322.1"
                     /translation="MQDSSLNNYANHKNFILMLIILFLMEFARGMYILSYINFLPTVT
                     SIAVAITSLAFSIHFIADASTNFVIGFLLKKFGTKIVLTTGFILAFTSLFLVIWFPAS
                     PFVIIFSAMMLGIAVSPIWVIMLSSVEEDKRGKQMGYVYFSWLLGLLVGMVFMNLLIK
                     VHPTRFAFMMSLVVLIAWILYYFVDVKLTNYNTRPVKAQLRQIVDVTKRHLLLFPGIL
                     LQGAAIAALVPILPTYATKVINVSTIEYTVAIIIGGIGCAVSMLFLSKLIDNRSRNFM
                     YGVILSGFILYMILIFTLSMIVNIHIVWIIALAIGLMYGILLPAWNTFMARFIKSDEQ
                     EETWGVFNSIQGFGSMIGPLFGGLITQFTNDLNNTFYFSALIFLVLAVFYGSYFIANR
                     EKAK"
     gene            complement(2631..3140)
                     /locus_tag="NBI57_002396"
     CDS             complement(2631..3140)
                     /locus_tag="NBI57_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjcG family protein"
                     /protein_id="HCD1823323.1"
                     /translation="MILGLALIPSKSFQEAVDSYRKRYDKQYSRIKPHVTIKAPFEIE
                     DGDLDSVIEQVRARINGIPAVEVHATKASSFKPTNNVIYFKVAKTDDLEELFNRFNGE
                     DFYGEAEHVFVPHFTIAQGLSSQEFEDIFGQVALAGVDHKEIIDELTLLRFDDDEDKW
                     KVIETFKLA"
BASE COUNT         1074 a          518 c          510 g         1136 t
ORIGIN      
        1 aataaaacat agaacaataa attgctttat aaaataacgt tttattttgt atcaattcat
       61 ctattgatta aaattgctat aaatgtaacg actttgcgtc tttatttctt ttataatgcc
      121 gtaaaataag ttacacttaa ataaaatttt caaactaacg gagggtggct attaatggtt
      181 actcaaaata aaaagatatt gattattact ggctcattcg gtaacggtca tatgcaagtt
      241 acacagagta tcgttaatca acttaatgat atgaatctag accatttaag cgtcattgag
      301 cacgatttat ttatggaagc tcatccaatt ttgacttcta tttgtaaaaa atggtatatc
      361 aatagcttta aatattttag aaatatgtac aaagggtttt attacagccg cccagataaa
      421 ctagacaaat gtttttacaa atactatgga cttaataagt taattaattt attgataaaa
      481 gaaaagccag atttaatatt attaacgttt cctacaccag ttatgtcggt actaactgag
      541 caatttaaca ttaatattcc agttgctaca gtgatgacag actatcgctt acataaaaac
      601 tggattacgc cgtattcaac aagatattat gtggcaacaa aagaaacgaa acaagacttc
      661 atagacgtag gtattgatcc ttcaacagtt aaagtgacag gtattcctat tgataacaaa
      721 tttgaaacgc ctattaatca aaagcagtgg ttaatagaca acaacttaga tccagataag
      781 caaactattt taatgtcagc tggtgcattt ggtgtatcta aaggttttga cacgatgatt
      841 actgatatat tagcgaaaag tgcaaatgca caagtagtta tgatttgtgg taagagcaaa
      901 gagctaaagc gttctttaat agctaagttt aaatctaacg agaatgtatt gattctaggt
      961 tataccaaac acatgaatga atggatggca tcaagtcaac ttatgattac gaaacctggt
     1021 ggtatcacaa taactgaagg tttcgcccgt tgtattccaa tgattttcct aaatcctgca
     1081 cctggtcaag agcttgaaaa tgccctttac tttgaagaaa aaggttttgg taaaatcgct
     1141 gatactccag aagaagcaat aaaaattgta gcaagtttaa cgaacggtaa tgagcaatta
     1201 actaacatga ttagtacaat ggaacaagat aaaattaagt atgctacaca gacaatatgt
     1261 cgagacttac ttgatttaat cggtcattca tcacaaccac aagagattta tggaaaggtt
     1321 cctttatatg caagattctt cgttaaataa ttacgctaat cataaaaatt tcattttaat
     1381 gcttattatc ttatttttaa tggaatttgc gagaggcatg tatatcctaa gctatattaa
     1441 tttcttgcca acagttacat ccattgctgt agcaataacg tctctagcat tttcaataca
     1501 ttttattgct gatgcatcta caaactttgt tataggattt ttactgaaaa aattcggcac
     1561 aaaaatcgtt ttaacgactg gatttatttt agcatttaca agtttatttt tagttatttg
     1621 gtttccagca tcaccttttg taatcatatt tagtgctatg atgctcggta ttgcagttag
     1681 tcctatttgg gtcattatgt tatccagtgt tgaagaagat aaacgtggca aacaaatggg
     1741 ctatgtttat ttttcatggt tgttaggcct attggtaggt atggttttta tgaatctact
     1801 tatcaaagtg cacccaacac gctttgcctt catgatgtct ttagtcgttt taattgcatg
     1861 gatattatat tattttgtcg atgtaaagtt aacaaattat aatactcgtc cagttaaagc
     1921 tcaattgaga caaattgttg atgtgacaaa gcgccattta ttgttgttcc ctggtattct
     1981 tttacaaggt gccgcaattg ctgccctagt tcctatatta ccaacatatg ctactaaggt
     2041 tattaacgta agcacaattg aatatactgt tgcaatcatt attggtggta tcggctgtgc
     2101 agtttcgatg ctatttttat cgaaactaat agataaccgc agcagaaact ttatgtatgg
     2161 tgttattcta agcggattta ttttatacat gatattaatt tttactctat ctatgattgt
     2221 taatattcac atcgtgtgga ttatcgcttt agctatcggt ctaatgtatg gcatcttatt
     2281 accagcatgg aataccttta tggcacgatt tatcaaatca gatgaacaag aagaaacttg
     2341 gggtgttttt aatagtatcc aaggattcgg ctcaatgata gggccattat ttggcggatt
     2401 aattacacaa tttaccaacg atttaaacaa tacattttat ttctcggcat taatcttttt
     2461 agtcttagct gtattttatg gaagctactt tatagcaaat agagaaaaag ctaagtaagg
     2521 ttaaaacgta ttttaaaata aagaaaatgg tcatttttat tacattcaat aaaaatgacc
     2581 attttcttat taattcaaac atactacgat taacaatact attatgttac ttaagctaat
     2641 ttaaacgttt caataacttt ccatttatct tcgtcatcgt caaaacgtaa caaagttaat
     2701 tcatcgataa tttctttatg gtctacccca gctaatgcta cttgaccaaa aatatcttcg
     2761 aattcttggc tagatagtcc ttgtgctatt gtaaagtgtg gcacaaaaac atgttcagct
     2821 tctccataga aatcttctcc attaaagcga ttaaacaatt cttctaagtc gtccgtcttc
     2881 gcaactttaa agtaaatcac attgttcgtt ggtttgaagc tagaagcttt tgtagcatga
     2941 acttctactg ctggtatacc attaatacga gctctaacct gttcaatgac agaatctaaa
     3001 tcaccatctt caatttcaaa tggcgcttta attgtcacat gtggtttaat tcgtgaatac
     3061 tgtttatcat atcttttacg gtaagaatcc accgcttctt gaaatgactt tgatggaatt
     3121 aatgctaatc ctaaaatcat ctgaattcct cctttgttta acctattgct attatatcaa
     3181 atttctttga aaaccgttac attaaaaata attcattata catcttttgc aatttcga
//
LOCUS       DAHHSC010000226         3224 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.230, whole genome shotgun sequence.
ACCESSION   DAHHSC010000226 DAHHSC010000000
VERSION     DAHHSC010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3224)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3224)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3224
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.230"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            154..504
                     /locus_tag="NBI57_002397"
     CDS             154..504
                     /locus_tag="NBI57_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YppE family protein"
                     /protein_id="HCD1823324.1"
                     /translation="MNDVVESLIYEVNNMQQNFENVKSQQQDHDFYQTVKPYTEHIDS
                     MLNEIKLHREFIIEVPYMNSRKFELLIANIEQLSVECHFKRTSRKLFIEKLKSVQYDL
                     QNILDGVTKEGTYG"
     gene            497..1060
                     /locus_tag="NBI57_002398"
     CDS             497..1060
                     /locus_tag="NBI57_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HCD1823325.1"
                     /translation="MVKTVYVTGYKSFELNIFKDDAPEVHYLKQFIKHKIEQLLDEGL
                     EWVLIQGQMGIELWTAEVVIELQRTYDSLKFAVITPFQGHTEKWNEHNQSKYANIIKH
                     ADYVDSIFHTSYQGPFQFKQADQFMLEHSDQTLLIYDEEQEASPKFFKQMLVDFMDKT
                     NYTCDIVTFDELTAFINDLQWSEDQSF"
     gene            1074..1418
                     /gene="gpsB"
                     /locus_tag="NBI57_002399"
     CDS             1074..1418
                     /gene="gpsB"
                     /locus_tag="NBI57_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HCD1823326.1"
                     /translation="MSDVSLKLSAKDIYEKDFEKTMARGYRREEVDAFLDDIIADYQK
                     MADMNNEVVKLSEENHKLKKELEELRLRVATSRPQDNKSFSSNNTTTNTSSNNVDILK
                     RISNLEKAVFGK"
     gene            1481..1860
                     /gene="rnpB"
                     /locus_tag="NBI57_002400"
     ncRNA           1481..1860
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="NBI57_002400"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            2069..3214
                     /locus_tag="NBI57_002401"
     CDS             2069..3214
                     /locus_tag="NBI57_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HCD1823327.1"
                     /translation="MFQLLAVCPMGLEAVVAREIQELGYETNVENGRIFFEGDASAIV
                     KANLWLRTADRIKIVVGRFNATTFDELFEQTKALPWESIIDKEGNFPVQGRSVKSTLH
                     SVPDCQAITKKAIVERLRRAYNEKGWLNESGAKYPVEVAILKDNVLLTIDTSGSGLNR
                     RGYRLAQGEAPIKETLAASLIRLANWKGDTPLIDPFCGSGTIAIEACLIAQNIAPGFN
                     REFVSEQWNIMPANIYDDYRDEADKMADYDKEIEVYASDIDPEMVEIAKRNAEEIGLS
                     DIIKFSVKDVNTLTIDTEEPVALIGNPPYGERIGDREEVEEMYRYIGKLMKQHPFLST
                     YILTSNKEFEYLVNRKATKRRKLFNGYIECTYYQYWGKKTERKTIEN"
BASE COUNT         1206 a          433 c          619 g          966 t
ORIGIN      
        1 gataaaattt tgtatttata taggaagttt ttagatttac ttgatttaac agataacaca
       61 tttgaaaaaa tagttaaatc aaattatata gagtgttaaa acatgacgaa tacagtatat
      121 tgtgataaat taaaaaatgt aggaggcgtc ctcatgaatg atgtagtcga atcactaatt
      181 tatgaagtta acaacatgca acaaaatttt gaaaatgtga aatcacaaca acaagatcat
      241 gatttttacc aaactgtaaa gccatatact gaacatattg atagcatgct caatgagatc
      301 aaattacatc gtgaatttat tatagaagta ccttatatga attcaaggaa atttgagcta
      361 ctgattgcta acattgaaca actttctgtc gaatgtcatt ttaagcgaac aagtcgaaag
      421 ttatttatag aaaagcttaa aagtgtccaa tatgatttac aaaatatatt agatggcgta
      481 acaaaagagg gtacttatgg ttaaaacagt ttatgtaaca ggttacaaat cattcgaatt
      541 aaacattttt aaagatgacg cacctgaagt acattattta aaacaattta taaaacataa
      601 aattgaacaa ctgttggatg aaggattaga atgggtgtta atacaagggc aaatgggtat
      661 agaattatgg actgcagaag ttgttattga attacaacga acatatgatt cgttaaagtt
      721 tgctgtaatt acaccgttcc aaggacatac agaaaagtgg aatgaacata atcaaagtaa
      781 gtatgccaac ataattaagc atgcagatta tgttgatagt atttttcata cgtcgtatca
      841 aggtcctttt caatttaaac aagcagatca atttatgctt gaacattcgg atcaaacatt
      901 gctcatttat gatgaggaac aagaggcaag tcctaagttc ttcaagcaga tgttagttga
      961 ttttatggat aaaacaaact atacttgtga tattgtgacg ttcgatgaat taacagcatt
     1021 catcaatgac ttacagtggt ctgaagatca aagtttctaa tgaggtggaa aaaatgtcag
     1081 atgtttcatt gaaattatca gcaaaagata tttatgaaaa agattttgaa aaaacgatgg
     1141 ctcgtggcta tagaagagaa gaagtagatg catttttaga tgacattatt gctgattatc
     1201 aaaaaatggc cgatatgaat aatgaagttg taaaattatc agaagagaat cataaactta
     1261 aaaaagaatt agaagaatta agactacgtg tagcaacatc aagacctcag gacaataaaa
     1321 gtttttcttc gaataataca acaacaaata catcttcaaa taatgtagat attttaaaac
     1381 gtatttcaaa cttagaaaaa gctgtatttg gtaaataatt actaaataca aaagtttaac
     1441 tgtcttacta ataatgacta tgttataatt ttaaaagtga tattttgggt aatcgctata
     1501 ttatatagag gaaagtccat gctcacacag tctgagatga ttgtagtgtt cgtgcttgat
     1561 gaaacaataa atcaaggcat taatttgacg gcaatgaaat atcctaagtc tttcgatatg
     1621 gatagagtaa tttgaaagtg ccacagtgac gtagctttta tagaaatata aaaggtggaa
     1681 cgcggtaaac ccctcgagtg agcaatccaa atttggtagg agcacttgtt taacggaatt
     1741 caacgtataa acgagacaca cttcgcgaaa tgaagtggtg tagacagatg gttatcacct
     1801 gagtaccagt gtgactagtg cacgtgatga gtacgatgga acagaacatg gcttatagaa
     1861 atatcactac tagtttagct ctcctagatg atggagagct tttttcatga aaagaacact
     1921 taaaattaac gccttgtctt gatataatga cactgccttg ttttaaaata gtaagcggat
     1981 gcgttaatgt atcagcgatt aaatttgttg gaaatgtata aaaaacacaa gctaagaata
     2041 aaatacctgt ataaaaggag aatcatatat gtttcaatta cttgcagttt gtccgatggg
     2101 attagaagct gttgttgcta gggaaattca agaattaggc tatgaaacaa atgttgaaaa
     2161 tggtcgtata ttttttgaag gagacgcaag tgcaattgta aaggcaaatt tatggttgcg
     2221 cacagcagac cgaatcaaaa ttgttgttgg acgttttaac gcaacaacgt ttgacgaatt
     2281 attcgaacaa accaaagcgc tcccttggga atctataatt gataaagaag gtaacttccc
     2341 agttcaaggt agaagcgtta aatcaacact acatagtgtg ccagattgtc aggcgataac
     2401 taagaaagct attgttgaac gattacgtcg cgcatataac gaaaaaggtt ggttaaatga
     2461 atcaggtgcc aaataccctg ttgaagttgc tattttaaaa gataatgtat tattgactat
     2521 cgacacatca ggttctggtt tgaacagacg tggttataga ttagcacaag gtgaagcacc
     2581 aattaaagaa acgttggcag caagtttaat ccgtcttgca aactggaaag gtgatacacc
     2641 tttaattgat ccattttgcg gttcgggtac aatagctata gaagcttgtt taattgctca
     2701 aaatatcgca cctggtttta atcgcgagtt cgtatcagag caatggaaca tcatgccagc
     2761 aaatatttat gatgattacc gtgatgaagc ggataagatg gctgattatg ataaagaaat
     2821 cgaagtatat gcttctgata tcgatccaga aatggtagag attgctaagc gtaacgctga
     2881 agaaattggg ttgtctgata ttattaaatt tagtgtaaaa gatgtcaata cattaacaat
     2941 tgatacagaa gaaccggtgg cgttaattgg aaatcctcca tatggtgaac gtattggtga
     3001 tcgtgaagaa gttgaagaaa tgtaccgtta tattggtaaa ctaatgaaac aacatccatt
     3061 tttatctaca tacattttaa caagtaataa agaatttgaa tatttagtaa atcgtaaagc
     3121 aacgaagcga cgtaagttat ttaatggata tatagaatgt acttattatc aatactgggg
     3181 taaaaagaca gaacgtaaaa ctattgaaaa ctaaagtgtt tcta
//
LOCUS       DAHHSC010000227         3189 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.231, whole genome shotgun sequence.
ACCESSION   DAHHSC010000227 DAHHSC010000000
VERSION     DAHHSC010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3189)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3189)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3189
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.231"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            164..2206
                     /gene="lip1"
                     /locus_tag="NBI57_002402"
     CDS             164..2206
                     /gene="lip1"
                     /locus_tag="NBI57_002402"
                     /EC_number="3.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK domain-containing triacylglycerol lipase
                     Lip1"
                     /protein_id="HCD1823328.1"
                     /translation="MKSQNKYSIRKFSVGASSILIATLLFLSGGQAQAAEKQVNMGNS
                     QEDTVTAQSIGDQQTRENANYQRENGVDEQQHTENLTKNLHNDKTISEENHRKTDDLN
                     KDQLKDDKNSSLNNKNIQRDTTKNNNANPSDVNQGLEQAINDGKQSKVASQQQSKEVD
                     NSQDSNANNNLPSQSLTKEAPSLNKSDQTSQREIVNETEIEKVQPQQNNQANDKITNH
                     NFNNEQEVKPQKDEKTLSVSDLKNNQKSPVEPTKDNDKKNGLNLLKSSAVATLPNKGT
                     KELTAKAKDDQTNKVAKQGQYKNQDPIVLVHGFNGFTDDINPSVLAHYWGGNKMNIRQ
                     DLEENGYKAYEASISAFGSNYDRAVELYYYIKGGRVDYGAAHAAKYGHERYGKTYEGI
                     YKDWKPGQKVHLVGHSMGGQTIRQLEELLRNGNREEIEYQKKHGGEISPLFKGNNDNM
                     ISSITTLGTPHNGTHASDLAGNEALVRQIVFDIGKMFGNKNSRVDFGLAQWGLKQKPN
                     ESYIDYVKRVKQSNLWKSKDNGFYDLTREGATDLNRKTSLNPNIVYKTYTGEATHKAL
                     NSDRQKADLNMFFPFVITGNLIGKATEKEWRENDGLVSVISSQHPFNQAYTNATDKIQ
                     KGIWQVTPTKHDWDHVDFVGQDSSDTVRTREELQDFWHHLADDLVKTEKVTDTKQA"
     gene            complement(2541..>3189)
                     /gene="icaC"
                     /locus_tag="NBI57_002403"
     CDS             complement(2541..>3189)
                     /gene="icaC"
                     /locus_tag="NBI57_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="polysaccharide intercellular adhesin
                     biosynthesis/export protein IcaC"
                     /protein_id="HCD1823329.1"
                     /translation="YIIFKINYNLFNSKILLLLSFILQQSFLYYFTNNTAFHDTVLHY
                     YPLSENTIIFGWIFYFFLGAYMGYNYERVLNFLERYLVIMIVLAVATYFVFIALANGD
                     YWNVTSFSYSLTPYNSIMFIVILGICTHFKTMLFNTIQMISAFSFFIYLLHPIILDSL
                     FAYTNIFEDNTMVFLAISLLFILGLCIGVGMILREFYIFRFIIGKQPYKLNINAY"
BASE COUNT         1297 a          470 c          560 g          862 t
ORIGIN      
        1 gagaatatat tttttaaata caaatgtaaa cgctttctcg tcaaattaaa caatagaaag
       61 gatggtcatt atgagtgctt ggttaagtaa attatttgag tttattcctc gaataattat
      121 caatttgttt atctaaacta aaaaaataga ggtgctgaca atgatgaaaa gtcaaaataa
      181 gtatagtatt cgtaaattta gtgtaggtgc atcttccatt ttaatagcta cattactatt
      241 tttaagtggt ggacaagcac aagcagctga gaagcaagtg aatatgggaa attcacagga
      301 ggatacagtt acagcacaat ctattgggga tcaacaaact agggaaaatg ctaattatca
      361 acgtgaaaac ggtgttgacg aacagcaaca tactgaaaat ttaactaaga acttgcataa
      421 tgataaaaca atatcagaag aaaatcatcg taaaacagat gatttgaata aagatcaact
      481 aaaggatgat aaaaattcat cgcttaataa taaaaatatt caacgtgata caacaaaaaa
      541 taacaatgct aatcctagcg atgtaaatca agggttagaa caggctatta atgatggcaa
      601 acaaagtaaa gtggcgtcac agcaacagtc aaaagaggta gataatagtc aagattcaaa
      661 cgctaataac aatctacctt cacaaagttt aacaaaagaa gcaccatcat taaataagtc
      721 agatcaaaca agtcaacgag aaattgttaa tgagacagaa atagagaaag tacaaccaca
      781 acaaaataat caagcgaatg ataaaattac taaccacaat tttaacaatg aacaagaagt
      841 gaaacctcaa aaagacgaaa aaacactatc agtttcagat ttaaaaaaca atcaaaaatc
      901 accagtagaa ccaacaaaag acaatgacaa gaaaaatgga ttaaatttat taaaaagtag
      961 tgcagtagcc acgttaccga ataaggggac aaaggaactt actgcaaaag cgaaagatga
     1021 tcaaacgaat aaagttgcca aacaagggca gtataaaaat caagatccta tcgttttagt
     1081 gcatggtttc aatgggttta cagatgatat taatccttca gtgttagctc attattgggg
     1141 cggtaataaa atgaacattc gccaagattt agaagaaaat ggttacaaag cttatgaagc
     1201 aagtataagt gcttttggaa gtaactatga ccgcgcagtt gaactttatt attatatcaa
     1261 aggcggtcgt gtagattatg gtgcagcaca tgcagcaaaa tatggacatg aacgttatgg
     1321 aaaaacatac gaaggaattt acaaagactg gaaaccagga cagaaggtac acctagttgg
     1381 acatagtatg ggcggtcaaa cgatacgtca actagaagaa ttactgcgta atggtaatcg
     1441 tgaagaaata gagtatcaaa agaaacatgg tggcgaaatt tctccactat tcaaaggtaa
     1501 taatgacaac atgatttcat caattactac tttaggaaca ccacataatg gtacacacgc
     1561 atcagattta gctggtaatg aagctttagt gagacaaatc gtatttgata tcggtaaaat
     1621 gtttggtaat aaaaattcaa gagtagactt cgggttggct caatggggtc taaaacagaa
     1681 gccaaatgaa tcatatattg attatgtcaa acgcgttaaa caatctaatt tatggaaatc
     1741 aaaagataat ggattttacg atctgacgcg tgagggtgca acagatttaa atcgtaaaac
     1801 gtcgttgaac cctaacattg tgtataaaac atacactggt gaagcaacgc acaaagcatt
     1861 aaatagcgat agacaaaaag cagacttaaa tatgtttttc ccatttgtga ttactggtaa
     1921 cttaatcggt aaagcaactg aaaaagaatg gcgagaaaac gatggtttag tatccgttat
     1981 ttcttctcaa catccattta atcaagctta tacaaatgcg acggataaaa ttcaaaaagg
     2041 catttggcaa gtgacgccta caaaacatga ttgggatcat gttgactttg taggacaaga
     2101 cagttctgat acagtgcgca caagagaaga attacaagat ttttggcatc atttagcaga
     2161 cgatttagtg aaaactgaaa aggtgactga tactaagcaa gcataattta taaagtaaag
     2221 ggaggtatat acaatgacag cagacttctt tcaattaatc ggatcattat ttagaattct
     2281 aaaagaatta ttcaagtaat atcttggcgg ggccccaaca aaaagaattt cgaaaagaaa
     2341 ttctacaaac aatgcaagtt ggggtgggac gacgaaataa attttgcgaa aatatcattt
     2401 ctgtcccact cccattggca tttacgaagt ttaaatgtgc aattagaata tatgtataac
     2461 attattaaac acgcggtaaa acgaagtcag tcaattcaaa ctgatttcgc ccaccgcgtg
     2521 tttttaacat agcttaataa ttaataagca ttaatgttca atttatatgg ttgttttcca
     2581 ataataaacc taaagatata gaattcacgc aatatcatac cgacacctat acataaccct
     2641 aaaatgaata gtagtgatat cgctagaaag accattgtat tatcctcaaa tatatttgta
     2701 tatgcaaaca atgagtctag aatgattgga tgtaataaat aaataaagaa tgagaaagca
     2761 ctaatcattt gaatcgtatt aaataacatt gttttaaaat gcgtgcaaat acccaagata
     2821 acaataaaca taatactatt atatggtgtt aatgaatatg aaaagctggt aacgttccaa
     2881 tagtctccat ttgctaacgc aataaacaca aaataagtag ctacagctaa tacaatcata
     2941 ataactaaat aacgttctaa gaaatttaat acacgttcgt agttataacc catatatgca
     3001 cctaaaaaga aataaaaaat ccatccgaat attatagtgt tttcacttaa tggataatag
     3061 tgtagcacgg tatcgtgaaa cgctgtgttg ttcgtaaagt aatataaaaa tgattgctgt
     3121 aaaataaaag ataacaataa taatatttta ctgttgaata ggttatagtt aattttaaaa
     3181 atgatataa
//
LOCUS       DAHHSC010000228         3184 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.232, whole genome shotgun sequence.
ACCESSION   DAHHSC010000228 DAHHSC010000000
VERSION     DAHHSC010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3184)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3184)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3184
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.232"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            188..2338
                     /locus_tag="NBI57_002404"
     CDS             188..2338
                     /locus_tag="NBI57_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HCD1823330.1"
                     /translation="MDNQLINSIIEKYQFSKKQIEAVLTLLEEKNTVPFIARYRKEQT
                     GGLDEVQIKQIDDEYQYMVNLQKRKEEVIKNIEQQGLLTEELKKDILKQNKLQRVEDL
                     YRPFKQKKKTRATEAKRKGLEPLAIWMKARKHEVSIEEKAQQFINEEVQSVEDAIKGA
                     QDIIAEQISDNPKYRTKILKDMYHQGVLTTSKKKNAEDEKGIFEMYYAYSEPIKRIAN
                     HRVLAVNRGEKEKVLSVKFEFDTTSVEDFIARQEINHNNVNRSYILEAIKDSLKRLIV
                     PSIEREIHADLTEKAENHAIDVFSENLRNLLLQPPMKGKQILGVDPAFRTGCKLAVIN
                     PFGTFIAKGVIYPHPPVSKKEAAEKDFVQMVKAYDVQLIAIGNGTASRETEQFVADLI
                     KKHQLPVQFIIVNEAGASVYSASEIARDEFPDFQVEERSAVSIGRRVQDPLSELVKID
                     PKSIGVGQYQHDVNQKALENALTFVVETAVNQVGVDVNTASSSLLQYVSGLSSQIAKN
                     IIAYREENGAIKHNKELSKIKRLGAKTFEQSIGFLRIVDGSEPLDNTSIHPESYKVTY
                     QLLDKLGFGGNDLGSDALKAKLNSLDMDELAIELQVGVPTLEDIIKSLKAPNRDPRDE
                     FDTPILKSDVLSIEDLKEGMKLSGTVRNVVDFGAFVDIGVKQDGLVHVSKLSKKFVKN
                     PMDIVSVGDIVDVWVYSIDKNKDKVSLTMIDPHE"
     gene            2331..2786
                     /locus_tag="NBI57_002405"
     CDS             2331..2786
                     /locus_tag="NBI57_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HCD1823331.1"
                     /translation="MNNDKLQRMVENLSEEKFGRTFRHCAYFNKRLRTTGGRYLLKSH
                     DIEINPKQYEHYGEDAVVKIILHELCHYHLHIAGKGYQHKDQDFKRLSQQVGAPRFCN
                     SIESYQQRANYEYYCTKCHAKYIRIRKVDTNRMRCGHCNGKLRMKRQLK"
BASE COUNT         1216 a          405 c          613 g          950 t
ORIGIN      
        1 tgaaatttag aaatgatggt gtgtcatttt caataatctt agtgcgtttt aaaatatagt
       61 atgacctaat aattcgtttt aaatgttttg gaagtgaaaa ttacattaag tatcatacct
      121 taatagaagt attttagaat atgttaaaat aaatgagtaa agttaagaaa aagtgtgggt
      181 taagtaaatg gacaatcaat tgattaattc aatcatagag aaatatcaat ttagtaaaaa
      241 acaaattgaa gcagtattaa cactgctaga agaaaaaaat acagtaccat ttattgcgag
      301 gtatcgaaaa gagcaaactg gtggactaga tgaagttcaa ataaagcaaa ttgatgacga
      361 ataccaatat atggtcaatt tacaaaaacg taaagaagaa gttatcaaaa atatagaaca
      421 gcaaggatta cttactgagg aattaaagaa ggatatttta aaacagaaca aattacaacg
      481 tgttgaagac ctatataggc cttttaaaca aaagaaaaag acaagggcaa ctgaggcgaa
      541 acgtaaaggg ttagagccat tagcgatatg gatgaaggca cgtaaacatg aagtctcaat
      601 tgaagaaaaa gcacaacaat ttataaatga agaagtgcaa tcggttgaag atgctatcaa
      661 aggtgcacaa gatattattg cggaacaaat ttcagataat cctaaatata gaacaaaaat
      721 tttaaaagat atgtatcatc aaggtgtgtt aactacatct aaaaagaaaa atgctgaaga
      781 tgaaaaaggt atttttgaaa tgtactatgc atatagtgag ccaattaaac gcattgctaa
      841 tcatagagtt ttagctgtta atcgtggtga aaaagagaaa gtattatctg taaagtttga
      901 attcgatacg acatcagtag aggatttcat tgcacgtcaa gaaatcaatc ataataatgt
      961 aaatcgcagt tatattttag aggcgattaa agatagcttg aaacgcttaa ttgtcccttc
     1021 gatagagcgt gaaatccatg ctgatttaac tgaaaaagct gaaaatcatg caatagatgt
     1081 ttttagtgaa aacttaagaa atctattact gcaacctcca atgaaaggta aacaaatatt
     1141 aggcgtagat ccagcattta gaacaggttg taaattagca gtcattaacc cattcggtac
     1201 ttttatagca aaaggtgtga tttatccgca tccaccagtt tctaaaaaag aggcagcaga
     1261 gaaggatttt gtacaaatgg ttaaagcgta tgatgtgcaa ttaattgcaa ttggcaatgg
     1321 tactgcaagt cgtgaaacag aacaatttgt tgcagattta attaaaaagc atcagttgcc
     1381 agtacaattc atcattgtca atgaagcggg cgcttcagta tactcagcat cagaaattgc
     1441 tagagatgaa tttcctgatt ttcaagtgga agaaagaagt gccgtatcaa ttggacgacg
     1501 cgttcaagat ccattaagtg aattagttaa aatcgatcct aaatctattg gtgtcggaca
     1561 ataccagcat gatgttaatc aaaaagcact agaaaatgca ttaactttcg ttgttgaaac
     1621 agcagttaac caagtaggtg ttgatgtcaa tacagcatca tcatcattat tacaatatgt
     1681 gtctggtcta agttcgcaaa ttgctaaaaa tataattgca tatagagaag aaaatggcgc
     1741 aataaagcat aataaagagt taagcaagat taagcgtttg ggtgctaaaa cttttgagca
     1801 gagtatagga tttttaagaa tagtagacgg ttcagagcca ttggataata catcgattca
     1861 tcccgaaagc tataaagtaa cgtatcagtt attagataaa ctcggatttg gtggcaatga
     1921 tttaggtagt gatgcattaa aagctaaatt aaattcattg gatatggatg aactggcgat
     1981 tgaattacaa gtcggtgtac ctacattaga ggatattatt aaatccttga aagcacctaa
     2041 tcgagaccca agggacgagt ttgatacacc aatccttaaa tccgatgtgc tatcaattga
     2101 agatttaaaa gaaggaatga aattgagtgg cacagttaga aatgtagtag attttggagc
     2161 atttgtagat ataggtgtta aacaagatgg acttgtccat gtttcgaaac tttctaagaa
     2221 gttcgtaaaa aatccaatgg atattgtcag tgttggtgat atcgttgacg tttgggttta
     2281 cagtattgac aaaaataaag acaaagtatc attaacgatg attgatccac atgaataatg
     2341 acaaacttca gcgaatggtt gagaaccttt cggaagaaaa atttggacgt acgtttcgac
     2401 attgtgcata ttttaataaa aggttacgta caacaggcgg acgttatctt ttaaagtccc
     2461 atgatattga aattaatcca aaacaatatg agcattatgg agaagatgct gttgtaaaaa
     2521 ttatactgca cgaattgtgt cattatcact tgcatattgc tggtaaaggt tatcaacata
     2581 aagatcaaga ttttaaacga ttgagccaac aggttggagc gcctagattt tgtaacagca
     2641 ttgaaagcta tcaacaacgg gcgaattatg aatattattg tactaaatgt catgcaaaat
     2701 atatacgtat ccgtaaagtt gatacgaatc gtatgcgttg cggacattgc aatggaaaat
     2761 taagaatgaa aagacaattg aaataagtac aatacaaata gagctaattc gtgtatatgt
     2821 aagattattt atgagttgta tggaatttct atagaattta tcagtgagtt attttgattg
     2881 gtaaacatag taattgaata agaagaaatt gtgtttataa aatgtttaaa gccgagattg
     2941 tcgtaattta atacttcgca atctcggctt ttctacttat gatataggta atggcataaa
     3001 ttgtgacttg ataggatgaa attaaattga ttagattaaa aatcttgacg tattttagta
     3061 ataaataata tataaacttt acccaattaa agctttaaag tagcacaaaa gataattata
     3121 catttttaat gacattttga ttgaactttt ctattgactg tacagcttat agttagtatg
     3181 atta
//
LOCUS       DAHHSC010000229         3161 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.233, whole genome shotgun sequence.
ACCESSION   DAHHSC010000229 DAHHSC010000000
VERSION     DAHHSC010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3161)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3161)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3161
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.233"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            63..296
                     /locus_tag="NBI57_002406"
     CDS             63..296
                     /locus_tag="NBI57_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 domain-containing protein"
                     /protein_id="HCD1823332.1"
                     /translation="MDYIGQYAVIHLVLHVVCICIAYWALQSIRLDQFFKKGYATQLQ
                     VCMIFVAILLGTAVSNFIVDLLQYSTQVKYLIK"
     gene            407..1672
                     /gene="murA"
                     /locus_tag="NBI57_002407"
     CDS             407..1672
                     /gene="murA"
                     /locus_tag="NBI57_002407"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HCD1823333.1"
                     /translation="MDKIVIKGGNKLTGEVKVEGAKNAVLPILTASLLASDKPSKLVN
                     VPALSDVETINNVLTTLNADVTYKKDENAVVVDATKTLNEEAPYEYVSKMRASILVMG
                     PLLARLGHAIVALPGGCAIGSRPIEQHIKGFEALGAEIHLENGNIYANAKDGLKGTSI
                     HLDFPSVGATQNIIMAASLAKGKTLIENAAKEPEIVDLANYINEMGGRITGAGTDTIT
                     INGVESLHGVEHAIIPDRIEAGTLLIAGAITRGDIFVRGAIKEHMASLVYKLEEMGVE
                     LDYQEDGIRVRAEGELQPVDIKTLPHPGFPTDMQSQMMALLLTANGHKVVTETVFENR
                     FMHVAEFKRMNANINVEGRSAKLEGKSQLQGAQVKATDLRAAAALILAGLVADGKTSV
                     TELTHLDRGYVDLHGKLKQLGADIERIND"
     gene            1706..2146
                     /gene="fabZ"
                     /locus_tag="NBI57_002408"
     CDS             1706..2146
                     /gene="fabZ"
                     /locus_tag="NBI57_002408"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HCD1823334.1"
                     /translation="METIFDYNQIKQIIPHRQPFLLIDKVVEYEEGQRCVAIKQVSGN
                     EPFFQGHFPEYAVMPGVLITEALAQTGAVAILNSEENKGKIALFAGIDKCRFKRQVVP
                     GDTLTLEVEITKIKGPIGKGNAKATVDGQLACSCELTFAIQDVK"
     gene            complement(2205..2645)
                     /locus_tag="NBI57_002409"
     CDS             complement(2205..2645)
                     /locus_tag="NBI57_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000846746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YwpF-like family protein"
                     /protein_id="HCD1823335.1"
                     /translation="MKTFKAVRFQIVNEHGRIIEYELEDGVIINKEESGTGWLLEIVI
                     SNEHYETFKEYQDNEQLLDIRVVITRPANDPALFESTVKSIKNFKTTMSIVFECHIYT
                     LRQQYAESLLEQLIDDGLSGEELKKSFNRMMQSKPKLKDEKLEE"
     gene            2839..>3161
                     /locus_tag="NBI57_002410"
     CDS             2839..>3161
                     /locus_tag="NBI57_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HCD1823336.1"
                     /translation="MLNKIVIVGRLTKDAQIFEKEDRKIATFCVATHRNYKDENGEIV
                     CDYLFCKAFGKLASNIEKYTNQGTLVGITGQMRSRKYDKDGQTHFVTELYVETIKFMS
                     PKSQN"
BASE COUNT         1123 a          446 c          575 g         1017 t
ORIGIN      
        1 tttgtatcat tatttaaatt tttcatgtac aatgtaatac agtaatctta tgaggtgata
       61 aaatggatta tatcggacaa tatgcagtta tccatttagt gttacatgtt gtatgtattt
      121 gtattgccta ttgggcttta caatcaatta gattagatca attttttaaa aaaggatacg
      181 ccactcaatt acaagtgtgt atgatatttg ttgctatttt attaggcact gcagtaagca
      241 attttattgt agatttgtta caatactcga cgcaggtaaa atatttaata aaataagtct
      301 aactctatga tttgtaatca aaactagata taattaaata atgacttaaa ataattttaa
      361 aatagggaaa tgtaaagtaa taggagttct aagtggagga tttacgatgg ataaaatagt
      421 aatcaaaggt ggaaataaat taacgggtga agttaaagta gaaggtgcta aaaatgcagt
      481 attaccaata ttgacagcat ctttattagc ttctgataaa ccgagtaaat tagttaatgt
      541 tccagcttta agtgatgtag aaacaataaa taatgtatta acaactttaa atgctgacgt
      601 tacatacaaa aaggacgaaa atgctgttgt cgttgatgca acaaagactc taaatgaaga
      661 ggcaccatat gaatatgtta gtaaaatgcg tgcaagtatt ttagttatgg gacctctttt
      721 agcaagacta ggacatgcta ttgttgcatt gcctggtggt tgtgcaattg gaagtagacc
      781 gattgagcaa cacattaaag gttttgaagc tttaggcgca gaaattcatc ttgaaaatgg
      841 taatatttat gctaatgcta aagatggatt aaaaggtaca tcaattcatt tagattttcc
      901 aagtgtagga gcaacacaaa atattattat ggcagcatca ttagctaagg gtaagacttt
      961 aattgaaaat gcagctaaag aacctgaaat tgtcgattta gcaaactaca ttaatgaaat
     1021 gggtggtaga attactggtg ctggtacaga cacaattaca atcaatggtg tagaatcatt
     1081 acatggtgta gaacatgcta tcattccaga tagaattgaa gcaggcacat tactaatcgc
     1141 tggtgctata acgcgtggtg atatttttgt acgtggtgca atcaaagaac atatggcgag
     1201 tttagtctat aaactagaag aaatgggcgt tgaattggac tatcaagaag atggtattcg
     1261 tgtacgtgct gaaggggaat tacaacctgt agacatcaaa acactaccac atcctggatt
     1321 cccgactgat atgcaatcac aaatgatggc attgttatta acggcaaatg gtcataaagt
     1381 cgtaaccgaa actgtttttg aaaaccgttt tatgcatgtt gcagagttca aacgtatgaa
     1441 tgctaatatc aatgtagaag gtcgtagtgc taaacttgaa ggtaaaagtc aattgcaagg
     1501 tgcacaagtt aaagcgactg atttaagagc agcagcagcc ttaattttag ctggattagt
     1561 tgctgatggt aaaacaagcg ttactgaatt aacgcaccta gatagaggct atgttgactt
     1621 acacggtaaa ttgaagcaat taggtgcaga cattgaacgt attaacgatt aattcagtaa
     1681 attaatataa tggaggattt caaccatgga aacaattttt gattataacc aaattaaaca
     1741 aattatacct cacagacagc catttttatt aattgataaa gtagttgaat atgaagaagg
     1801 tcaacgttgt gtggctatta aacaagtatc aggaaacgaa ccattctttc aagggcattt
     1861 tcctgagtat gcggtaatgc caggcgtatt aattactgaa gcgttagctc aaacaggtgc
     1921 ggtagctatt ttaaatagtg aagaaaataa aggtaaaatc gctttatttg ctggtattga
     1981 taaatgtcgt tttaaacgtc aagtagtacc tggtgatact ttaacgttgg aagtagaaat
     2041 cactaaaatt aaaggaccaa tcggtaaagg taatgctaaa gctactgtcg atggtcaact
     2101 tgcttgtagt tgtgaactta catttgcaat tcaagatgta aaataaaaca aaaaaaacat
     2161 tcaaagattt aatgtgttgg cataatcttt gaatgttttt tattttactc ttctaatttt
     2221 tcatccttta actttggttt agactgcatc attcgattaa atgatttttt taattcttca
     2281 ccagataatc catcatcaat aagttgttct aataaacttt cagcatactg ttgacgtaaa
     2341 gtgtaaatat gacattcaaa tacgatagac attgttgttt taaaattttt aatacttttc
     2401 acagtagatt caaataaagc tggatcattg gctggacgcg tgatgacgac acgaatatcc
     2461 aataactgtt cattgtcttg atattcctta aatgtttcat aatgctcatt agaaatgaca
     2521 atttcaagca accaacccgt gccactttcc tctttattaa ttattacgcc atcttctaat
     2581 tcatattcta taattctgcc gtgttcattc acaatttgaa accttactgc tttaaaagtt
     2641 ttcatattcc cacatcccat tagtttaata tttatgattt ttgaattatt tagaagattt
     2701 aaggtcattt catgactaat ttctttaaat ataacaaata acgagatgct atagctatta
     2761 attatataaa atttgccatt ttgaccattt ttaagctagt aattaagata taggcatagg
     2821 aggtgaatca cacactacat gctaaataaa atcgtaattg tcgggagact gacgaaagac
     2881 gcacaaatat ttgaaaagga ggatagaaaa attgcaacgt tttgtgttgc aacgcaccga
     2941 aattataaag atgaaaatgg agaaatcgtc tgtgattact tattctgtaa agcatttggc
     3001 aagttagctt ctaatataga aaaatatact aatcaaggta cattggttgg tataactggt
     3061 caaatgagat caagaaagta tgataaagac ggacaaacac actttgtcac tgaattatat
     3121 gttgaaacaa taaaatttat gtcccctaaa tcccaaaata a
//
LOCUS       DAHHSC010000230         3159 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.234, whole genome shotgun sequence.
ACCESSION   DAHHSC010000230 DAHHSC010000000
VERSION     DAHHSC010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3159)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3159)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3159
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.234"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            352..1035
                     /locus_tag="NBI57_002411"
     CDS             352..1035
                     /locus_tag="NBI57_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superantigen-like protein SSL11"
                     /protein_id="HCD1823337.1"
                     /translation="MKLKNIAKASLALGILTTGMITTTAQPVKASTLEVRSQATQDLS
                     EYYKGRGFELTNVTGYKYGNKVTFIDNSQQIDVTLTGNEKLTVKDDDEVSNVDVFVVR
                     EGSDKSAITTSIGGITKTNGTQHKDTVQNVNLSVSKSTGQHTTSVTSEYYSIYKEEIS
                     LKELDFKLRKHLIDKHDLYKTEPKDSKIRITMKNGGYYTFELNKKLQPHRMGDTIDSR
                     NIEKIEVNL"
     gene            1057..2544
                     /locus_tag="NBI57_002412"
     CDS             1057..2544
                     /locus_tag="NBI57_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKLRK protein"
                     /protein_id="HCD1823338.1"
                     /translation="MRENFKLRKMKVGLVSVAITMLYIMTNGQAEASETNQKVSTNQE
                     SKAVSQTEQNSKETKAAESSKNFVNLDPIKPGAQKVTGTTLPNHIILLNIDGKSADSV
                     EGGYGDFITANDKGEFEYPLNNRKIVHNQEIEVSSSSPDLGDDEEDEEVEESSTEKAG
                     AEEESTDAKATYTTPRYEKAYEIPKEQLKEKDGHHQVFIEPITEGSGIIKGHTSVKGK
                     VALSINNKFINFEERAKGGISKEDTKASSDGVWMPINEKGYFDFDFKKNPFDNLELKK
                     NDEISLTFAPDDEDEALKSLIFKTKVTSLEDIDKAETKYDHTKVEKVKVLKDVKEDIH
                     VDEIYGSLYHTEKGKGILDKQGTKEITGKTKFANAVVKVYSDLGDAQLFPDIQVDENG
                     KFSFDAEKAGFRLQNGETLNFAVVKPITGDLLHQGFVSKYIDVYESPEEKKEREFEEK
                     LENTPAYHKLHGDKIVGYDVQGNPSTWFYPLGEKKVERTTPKLEK"
     gene            complement(2653..2961)
                     /gene="spn"
                     /locus_tag="NBI57_002413"
     CDS             complement(2653..2961)
                     /gene="spn"
                     /locus_tag="NBI57_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="myeloperoxidase inhibitor SPIN"
                     /protein_id="HCD1823339.1"
                     /translation="MKFKKVLVATAMVGVLATGVVGYGNQADAKVYSQNGLVLHDDAN
                     FLEHELSYIDVLLDKNADQATKDNLRSYFADKGLHSIKDIINKAKQDGFDVSKYEHVK
                     "
BASE COUNT         1248 a          399 c          556 g          956 t
ORIGIN      
        1 aaatttgtat aatttaacat aaaagttgta aaagtaaagt gaattaaaaa cgaacattaa
       61 atttaggcac tgtgaaagcg cagtgtcttt tttgtgtcga aattgtgtac agaataagta
      121 gttaaataaa gattaagttg agataaagtg ttattcgtaa ataaaagaga gtagatcgat
      181 aggaattgaa tgatattagt taactattta ttaaattact taataatgat taatttttag
      241 ttaaagtaag tttaatgtga agcacgacca ttgctcatta taatgaatga ggattgttcg
      301 tattgcgtaa tagaataaat caaatagact aaaaattggg agcatagaat tatgaaatta
      361 aaaaatattg ctaaagcaag tttagcacta gggattttaa caacaggtat gattacaact
      421 actgctcagc cagtaaaagc aagtacatta gaggttagat cacaagctac tcaagacttg
      481 agtgaatatt ataaagggag aggatttgaa ttaacaaatg taacaggata taaatacgga
      541 aataaagtta catttataga taattctcaa caaattgatg ttacattgac aggaaatgaa
      601 aaattaactg ttaaagatga tgacgaagtt tctaatgttg acgtgtttgt agtaagagaa
      661 ggtagtgaca aatcagctat cacaacatcg attggtggaa ttacaaagac aaatgggact
      721 caacataaag atactgttca aaacgttaat ttgtcagttt ctaagagtac aggtcaacac
      781 actacttctg tgacttcaga atactatagc atctataaag aagaaatctc attaaaagaa
      841 cttgatttta aattaagaaa gcatttaatt gataaacatg atctttataa gacagaacct
      901 aaagacagta aaattagaat tactatgaaa aacggtggct attatacatt tgaattaaat
      961 aaaaaattac agcctcatcg catgggtgat acgattgata gtagaaatat agagaaaatt
     1021 gaagtgaatt tataataata ttcgagggag tatatcatga gagaaaattt taaattacgt
     1081 aaaatgaaag tcggtttagt atcagttgca attactatgt tatatatcat gacaaacgga
     1141 caagctgaag catctgaaac taatcagaag gtaagtacaa atcaagaatc aaaagctgtt
     1201 tcgcagactg aacaaaattc caaagaaaca aaagcggcag agtcttctaa aaactttgta
     1261 aatttagatc ctatcaaacc aggagctcaa aaagtaacag gtactacatt accgaatcat
     1321 attattttac ttaatataga tggtaaaagt gctgattcag ttgaaggagg atatggtgat
     1381 tttataactg caaatgacaa aggagagttt gagtatcctt taaataatcg taaaattgtc
     1441 cataaccaag aaattgaggt atcatcatca agccctgatt taggtgacga tgaagaggat
     1501 gaagaagtag aagaatcttc aactgaaaaa gctggtgctg aggaagaaag tacagatgct
     1561 aaagctacat atacaacacc acgatatgaa aaagcgtatg aaataccgaa agaacaacta
     1621 aaagaaaaag atggacacca ccaagttttc atcgaaccaa ttactgaagg ttcaggtatt
     1681 attaaaggtc atacgtctgt aaaaggtaaa gttgctctat ctattaataa taaatttatt
     1741 aattttgaag aaagagctaa aggtggaatt agtaaagaag atactaaagc tagttccgat
     1801 ggtgtttgga tgcctattaa tgaaaaagga tattttgatt ttgatttcaa aaaaaatcct
     1861 tttgataact tagagttaaa gaaaaatgat gaaatctcat taacatttgc acctgatgat
     1921 gaagatgaag cattgaagtc attaattttc aaaactaaag taacgagctt agaagatatc
     1981 gataaagcag aaacaaaata tgaccatact aaagtggaaa aagtaaaagt attgaaagat
     2041 gttaaagaag atatacatgt agatgaaatt tacggaagct tatatcatac agaaaaaggt
     2101 aaaggtattc ttgataaaca aggaactaaa gagattacag ggaagactaa attcgcgaat
     2161 gcagtagtga aagtatattc tgacttaggt gatgcgcaat tgttccctga tattcaagta
     2221 gatgaaaatg gtaaatttag ctttgatgct gaaaaagctg gtttcagatt gcaaaatgga
     2281 gaaacattga attttgcagt agttaaacct attactggtg acctattaca tcaaggattt
     2341 gtctctaaat atattgatgt atatgaatct ccggaagaaa agaaagaacg tgaatttgaa
     2401 gaaaaacttg aaaacacgcc tgcatatcat aaattacatg gtgataaaat tgtaggatat
     2461 gatgttcaag gtaatccatc aacttggttc tatccattag gtgaaaagaa agttgaacgt
     2521 acaacaccta aattagaaaa ataattaata aaataaaaca gcttaatgat gtaatgaaat
     2581 tagtgagtta atcactgact tcaacgtcat tgagctgttt tttatttgct tcgttacaaa
     2641 gcattattga atttatttta cgtgttcata ttttgaaaca tcaaagccgt cttgcttagc
     2701 tttgttgata atgtctttga ttgaatgtag tcctttatcg gcgaagtatg atcttaagtt
     2761 gtcttttgta gcttggtcag cattcttatc taataacaca tcgatatagc ttaattcatg
     2821 ttctaagaag tttgcgtcat catgtagtac gagtccattt tgagaataaa ctttcgcatc
     2881 tgcttgatta ccatatccaa caacgccagt tgctaataca cctaccattg ccgtagctac
     2941 taaaaccttt ttaaatttca tatctatcac tcctctaaaa attgtaactc tatcataaca
     3001 ctgaatatta agaaaattac gtttattaag tcgatttaat aatttttaat aaatagttaa
     3061 actgacaaat attgcttaaa tgcaattaat ctttaaaacg gtgtttatgg atttttccta
     3121 ctaaaacctt gatttcaaaa agggtttaaa tcaaatgaa
//
LOCUS       DAHHSC010000231         3153 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.235, whole genome shotgun sequence.
ACCESSION   DAHHSC010000231 DAHHSC010000000
VERSION     DAHHSC010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3153)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3153)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3153
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.235"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            <1..954
                     /locus_tag="NBI57_002414"
     CDS             <1..954
                     /locus_tag="NBI57_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000554504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCD1823340.1"
                     /translation="RYANMSQLDLMIDLGAYRKDRSSKDKQYKMTTACLLFFGKYNAI
                     SDRFPGFQLDYFKKTNYLDTDWKDRISSGDLGNEDLNVYSFFEKVLIKLTDNIEESFS
                     LNDGLTRQNYARDLKVAIREALVNTLMHAYYDTKQSIKIVNCEDFIEFYNPGNMRINK
                     EDFIHGGHSKDRNSILSTLFRRVGYSEKAGSGGPRIFDVVNRHKLKTPEIELTDMDTN
                     VVLWKQDLMKEFEKYPELDKKVIKYIIDYGSISKGEALKMENMTEYQFRNILKKLKDD
                     NLIKKEGEGPATKYVLIESKEADILRTKKVIKSLESFFRNK"
     gene            complement(1327..2645)
                     /gene="cstB"
                     /locus_tag="NBI57_002415"
                     /pseudo
     CDS             complement(1327..2645)
                     /gene="cstB"
                     /locus_tag="NBI57_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002488497.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="persulfide dioxygenase-sulfurtransferase CstB"
     gene            2701..2838
                     /locus_tag="NBI57_002416"
     CDS             2701..2838
                     /locus_tag="NBI57_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001791883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1823341.1"
                     /translation="MELKLLNKLDDDQLDLEFLQDNGLKGNVQGPMTLKEPLKSYIQK
                     S"
     gene            2835..>3153
                     /locus_tag="NBI57_002417"
     CDS             2835..>3153
                     /locus_tag="NBI57_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001549968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HCD1823342.1"
                     /translation="MKTNILNLDNELENTEYVEGKPYVQYGIKYERNQALRNEAIKIH
                     GTTCKVCGFDFKAKYGDLGEGFIEIHHLKPMFSIKREIKVNPQKDLVPLCSNCHKMIH
                     RNTK"
BASE COUNT         1175 a          406 c          543 g         1029 t
ORIGIN      
        1 agatatgcga atatgagcca actggattta atgatagatt taggagcata tagaaaagat
       61 agaagttcga aagacaaaca gtataaaatg actacagcat gtttattatt ctttggtaag
      121 tataatgcga ttagtgatag attcccagga tttcaattag attattttaa gaaaacaaat
      181 tacctagata ctgattggaa agatagaata tcaagtggag atttaggtaa tgaagattta
      241 aacgtgtata gtttttttga aaaagtattg ataaaattaa ctgataacat tgaggaatca
      301 tttagcctaa atgatggttt gactagacaa aattatgcaa gagatttaaa agtagcaatt
      361 cgcgaagcac tggttaatac attaatgcat gcgtattatg atactaagca aagtattaaa
      421 atagttaatt gtgaagattt tatagagttt tataatccgg gtaatatgag aataaataaa
      481 gaagatttta ttcatggagg gcattcaaag gacagaaata gtatattatc gacgcttttc
      541 agaagagtag gatattcaga aaaagctgga tctggaggac caaggatatt cgatgtagtt
      601 aatagacata agcttaaaac gcctgaaata gaattaacgg acatggacac taatgtagta
      661 ctttggaaac aagatttaat gaaggagttt gaaaaatatc ctgagttaga caaaaaagta
      721 ataaagtata ttattgacta tggatcaata agtaagggtg aagccttaaa aatggaaaat
      781 atgacagaat atcagtttag aaatatttta aaaaaactaa aagatgataa cttgataaaa
      841 aaagaaggtg aaggtccggc tactaaatat gtgttaatag aatcaaaaga agctgatata
      901 ttgcgaacta aaaaagtaat taaaagttta gagtctttct ttaggaataa ataaaaaaac
      961 aagagatagg tgcgaagtgt ttggattata caaatgcttc gcatcttata tttattaata
     1021 aatatcatag aaaaccgtat cattaaccga tacgcagaga tgcggttttt tagacacttc
     1081 ataaagggat tttgaacgta tcagaacata tgaggtttat aggaattgct gttatgtttt
     1141 ttgatcacat caataaacaa aaaaggtatg tactatgtaa aatatttatt aaatgatata
     1201 aagcgagggt atataaatga tttttaaata gatatttatc caataatata aaaaggaact
     1261 ataagctata tctaaaagct atatctaact acttatagtc cttcttcatt agtataaata
     1321 taattattaa gaaaggtata tatctttgta accttcgttt acattaataa tgttgtgata
     1381 acctttgtgt tctaaaatac caatagctat agaacttcta atgccagact gacagtctac
     1441 ataaataaca tcgtttctat tgaaaggtaa atctgtttct aaaagtttgc cgtgtggcac
     1501 atgaaccgct tgggataagt ggccattatt ccattcatta tcattacgta catctaagac
     1561 atgtgcttca ttaccagtta tgtctttact atgaacagat tgtgtttgaa tttgagcttg
     1621 tggtaactga tatccagaca cattatcata tccaataagt tgtaaagtat gttatgtgtt
     1681 gcttttgaaa caaggtgata gtctccaatc aagttaattt cttgattata gtttagatac
     1741 cagccaattt gattgatgaa atttttatca tatggaatat tgattgtacc ttcaatatgt
     1801 ccaccatgat aagcctcctt actgcggaga tcaaaagtta atctgtttgt acttgtagct
     1861 ggataaaccg tataaggttg atataaattc ataccgaatt gattaatttt tttcatttgt
     1921 gcaaaatgat gtggtggtgc aggttggtca gaaatgagtt tatcgataaa ggtagcttca
     1981 ttattttcag aaaaagccca gttcgtttgt ttttcatagc caagcgtaga tgttggaata
     2041 gcacctaaag atttaccaca aggactacca gcgccatgac caggccaaat ttgaatgtag
     2101 tctggcaagt ctttaatact ttcaatagat ttaaacattt gttttgcgcc tatttcagat
     2161 aatccttcta ctttaacagc tttttctagt aaatcaggtc taccgatatc tcctacaaaa
     2221 ataaaatcac cactgaatag tcccattgga acttgtgctc cagcaccttc gtcagtaagt
     2281 aaaaaactta tactttctgg cgtgtgacca ggtgtatgaa gcacttttaa ttttatattt
     2341 cctacataaa tatcatcatt atgttgaaca aaatgagtgt ggttaggcat atttttataa
     2401 cctaacatgt catcactttc gcccgataca taaatactag catttaactt tatagcaaca
     2461 tctctaattc ctgaaacaaa atttgcatgt atatgtgttt cagctgcatg agtaatggtt
     2521 aaactctctt catcggcaac tcgaatatat gaagataagt cacaaatagg atcaatgatc
     2581 atggcttctc cagttttttg acaaccgatt aaataagatg cttgagataa atgtttatca
     2641 taaattgatg atagtcaatt tgttatcaag ggtgataatt atattaacta tggtaattac
     2701 atggaattaa agttattaaa caaacttgat gatgaccaat tagatttaga atttttacaa
     2761 gataatgggt taaaaggtaa tgttcaaggt cctatgactt taaaggaacc tttaaaaagt
     2821 tatatacaaa agtcttgaaa acgaatattt taaatttaga taatgagtta gaaaacactg
     2881 aatatgtcga aggtaaacca tatgtacagt acggtattaa atatgaaaga aatcaggcat
     2941 taagaaatga agctattaaa attcatggaa ctacatgtaa agtatgtgga tttgatttta
     3001 aagctaagta tggcgattta ggtgagggtt ttattgaaat tcatcattta aaaccaatgt
     3061 tttcaataaa aagagaaata aaagtaaatc cacaaaaaga tttagtccca ctatgttcta
     3121 attgccataa aatgattcat agaaatacta aaa
//
LOCUS       DAHHSC010000232         3148 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.236, whole genome shotgun sequence.
ACCESSION   DAHHSC010000232 DAHHSC010000000
VERSION     DAHHSC010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3148)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3148)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3148
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.236"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            211..966
                     /locus_tag="NBI57_002418"
     CDS             211..966
                     /locus_tag="NBI57_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent oxidoreductase"
                     /protein_id="HCD1823343.1"
                     /translation="MSDYVYNLVKKHHSVRKFKNKPLSEDVVKKLVEAGQSASTSSFL
                     QAYSIIGIDDEKIKENLREVSGQPYVVENGYLFVFVIDYYRHHLVDQHAETDMENAYG
                     STEGLLVGAIDAALVAENIAVTAEDMGYGIVFLGSLRNDVERVREILDLPDYVFPVFG
                     MAVGEPADDENGAAKPRLPFDHVFHHNKYHADKETQYAQMADYDQTISEYYDQRTNGN
                     RKETWSQQIEMFLGNKARLDMLEQLQKSGLIQR"
     gene            complement(1046..2434)
                     /locus_tag="NBI57_002419"
     CDS             complement(1046..2434)
                     /locus_tag="NBI57_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine transporter"
                     /protein_id="HCD1823344.1"
                     /translation="MNAFLTLINIIVLVIFIVILHMMARKHISFAKRVFTALGIGIVF
                     GVLLHLVYGTHSNVITSTSDWFNIVGQGYVALLQMIVMPLIFISIVAAFTKIQIGEKF
                     AKIGSLIFIFLIGTVTIAAIVGVVYALVFGLDASTINLGNAEQARGSEIAKQAKDLTA
                     HTLPQQILELLPKNPFLDFTGQRATSTIAVVIFASFIGFAYLRVARKQPDHGELLKRA
                     IDAIYSLVMAIVTFVLRLTPYGVLAIMANTLSTSDFGAIWTLGKFLIASYAALITMYI
                     IHLIILSLLGISPIRYVKKTLEVLIFAFTSRSSAGALPLNVQTQTRRLGVPEGIANFA
                     ATFGLSIGQNGCAGIYPAMLAIMVAPVANVEIDLQFIVTLIAVVIISSFGVAGVGGGA
                     TFASILVLSTLNLPVALAGVLISVEPLIDMGRTALNVNDSMLAGTGTAKLTKHWDKDT
                     FESNDNAALTSH"
     gene            2519..2617
                     /locus_tag="NBI57_002420"
     CDS             2519..2617
                     /locus_tag="NBI57_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1823345.1"
                     /translation="MQLNVNMMKFDRWHKGICKQIVLNLVEKGEKG"
BASE COUNT         1099 a          518 c          629 g          902 t
ORIGIN      
        1 aagtcaacct tattgataat ggtgtgagaa tatttgttcg agatggatga aggtgattag
       61 tgagaaactg gaattttcaa gtacgagaca atatttttaa aagttcaatt attaacttat
      121 aagcaaataa ttgctataaa aaagtttgga cgtgtacaat tgcaatatga agatttttaa
      181 ttaactgtaa agtatcgagg agtgggtaac gtgtcagatt atgtatataa tcttgtgaaa
      241 aagcatcatt ctgttagaaa atttaagaat aaacctttaa gtgaagacgt tgttaagaaa
      301 ttggtagaag ctggacaaag cgcttcgacg tcaagtttcc tacaagcata ctcaattatt
      361 ggtatcgacg atgagaagat taaagaaaat ttacgagaag tttctggaca accttatgtt
      421 gtagaaaatg gctatttatt cgtctttgtt attgattatt atcgtcatca tttagttgat
      481 caacatgctg aaactgatat ggaaaatgca tatggttcaa cggaaggttt gctagtaggt
      541 gcaatcgatg cagcattagt tgctgaaaat attgcggtaa ctgctgaaga tatggggtat
      601 ggcattgtct ttttaggatc attaagaaat gatgttgaac gagttcgaga aattttagac
      661 ttacctgact atgtcttccc ggtatttggt atggcagtag gggaacccgc agatgatgaa
      721 aatggtgcag ccaagccacg cttaccattt gaccatgtct tccatcataa taagtatcat
      781 gctgataagg aaacacagta tgcacaaatg gcagattacg accagacaat cagcgagtac
      841 tatgatcaac gtacaaacgg gaatcgcaaa gaaacatggt cgcagcaaat tgagatgttc
      901 ctaggaaaca aagcaagatt agatatgtta gaacaattgc aaaaatcagg cttaatacag
      961 cgatagcaag ataccaaaat aacccgcccc ctctagctta aaatgataag tatagctaga
     1021 gggggcgggt atttcttgca atgaattagt gtgaagttaa tgcagcatta tcatttgatt
     1081 cgaaagtatc tttgtcccaa tgttttgtta acttggcggt acctgtacca gctagcattg
     1141 aatcgttcac gtttaatgct gttctaccca tgtcaatcaa tggttcaacg gagatgagca
     1201 cgccggctaa agcgactggc aagtttaacg ttgacaacac caatatggat gcaaatgtag
     1261 ccccgccacc gacgccagca acgccgaatg aactaataat cacgacagcg attaacgtta
     1321 caataaattg taaatcaatt tctacattag cgacgggtgc gaccataatt gcaagcatgg
     1381 cagggtaaat gcctgcacaa ccattttgtc caatcgacaa tccaaatgtc gcagcgaaat
     1441 tggcaatacc ttctggcacg cctagacgtc ttgtttgtgt ttgtacattc aatggtaagg
     1501 cacccgcgct tgagcgtgat gtgaatgcaa agattaatac ttccaaagtc tttttaacat
     1561 agcgaattgg gctaatacct aacaggctta aaataattaa gtgaatgata tacatcgtaa
     1621 ttaatgcagc gtacgatgcg attaagaatt ttcctaaagt ccaaatggcg ccaaagtcac
     1681 ttgtcgataa tgtgttggcc ataattgcta atacaccgta tggcgttaaa cgtaagacga
     1741 acgtcacaat cgccattact agtgaataga tagcgtcaat cgcacgctta agcaattcac
     1801 catgatcagg ttgtttgcgt gctacgcgta aataagcaaa tcctataaac gaagcaaata
     1861 tcacgacagc aatcgtggaa gttgcacgtt gtccagtgaa atctaagaat ggatttttag
     1921 gcaataattc caaaatttgt tgtggtaacg tatgtgctgt taaatctttc gcttgtttag
     1981 caatttcgct tccacgtgct tgttcagcgt taccaagatt aattgttgat gcatctaaac
     2041 caaacaccaa ggcatacaca acaccaacaa ttgcagcaat ggtgacagtg ccaattaaaa
     2101 agataaaaat gagactacca attttagcaa acttttctcc gatttgaatt ttagtgaatg
     2161 cagcgacaat agaaatgaaa attaaaggca tcacaatcat ttgcaacaat gcaacgtaac
     2221 cttgtccgac aatgttgaac cagtcacttg ttgatgtaat aacattcgaa tgtgttccat
     2281 agacaagatg taataacacg ccaaatacaa tgccgatacc taaagctgta aacacacgtt
     2341 tcgcaaaaga tatatgtttg cgagccatca tgtgcaatat tacgatgaaa atcaccaata
     2401 caataatatt aatcagtgta agaaaagcat tcatgaacgt cactcctcaa atttttgaat
     2461 ataattccga ctagtatgct aagaataata ttaattttaa aatatttcaa gagggcattt
     2521 gcaattaaat gtaaacatga tgaaattcga ccgttggcat aagggaattt gtaaacaaat
     2581 cgttttgaat ttagtagaga aaggtgaaaa gggttaatag tgttatgacc tagtcgatta
     2641 aagttgatat ttgaaaatga agcagggcat taaatgcaat aaattaaata agttgtcatt
     2701 acagcgttaa taatagaaat gattttaaca ggaaaaaagt gatgaatatt tggaaaagat
     2761 atatatcgtg cactgtcata agagataaga tatgagaaaa caaatgcatc cttgaatcga
     2821 gatgtggcta tgacggtttg ataaaaattg ctaacctcat atttattgaa taaaaagata
     2881 ataaatgatc aaccttgaaa agacgcttcg aacaaacacg ttgtaaaaaa ttctgtcgaa
     2941 tcaaaattag catcttttga gggttcgtgt gtgcaggtgg tagttcaaaa gctaccgtaa
     3001 acaaatattt ttgtttgaga aaaaagacgt ttgtagtaaa tgtgaatatg agtgtgccct
     3061 tgtgtgattc gtcatttact aacacagcca aacatttaaa ttgaatgggc ttattaagat
     3121 gaatctattt aggaatacac aggagcca
//
LOCUS       DAHHSC010000233         3147 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.237, whole genome shotgun sequence.
ACCESSION   DAHHSC010000233 DAHHSC010000000
VERSION     DAHHSC010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3147)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3147)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3147
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.237"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            85..387
                     /locus_tag="NBI57_002421"
     CDS             85..387
                     /locus_tag="NBI57_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1823346.1"
                     /translation="MSRSKKYFYLSSLMIILSFFFNTNNVFLSGFFNSFIKLILFCSV
                     INSIVLILSIIFADRSIKSLKPDADWIRIASKSLPWIILIVILVHIFSIVRTFGFI"
     gene            643..1008
                     /gene="crcB"
                     /locus_tag="NBI57_002422"
     CDS             643..1008
                     /gene="crcB"
                     /locus_tag="NBI57_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HCD1823347.1"
                     /translation="MQYVYIFIGGALGALLRYLISFLNTDGGFPIGTLIANLTGAFVM
                     GLLTALTIAFFSNHPTLKKAITTGFLGALTTFSTFQLELIHMFDHQQFITLLLYAVTS
                     YVFGILLCYVGIKLGGGLS"
     gene            1005..1358
                     /locus_tag="NBI57_002423"
     CDS             1005..1358
                     /locus_tag="NBI57_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CrcB family protein"
                     /protein_id="HCD1823348.1"
                     /translation="MISIILVMIGGGFGAIARSAITDYFNHKFTSKLPIATLIVNLVG
                     SFLIGLTIGLSISISWFPAFFVTGFLGGLTTFSTLAKELTLMMTPKFDINLFLNYSLL
                     QFIIGFIACYIGYHI"
     gene            1757..3147
                     /locus_tag="NBI57_002424"
                     /pseudo
     CDS             1757..3147
                     /locus_tag="NBI57_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000277741.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182 family transposase"
BASE COUNT         1097 a          478 c          472 g         1100 t
ORIGIN      
        1 attgtgtcta caagttaata aaactattgt tttatgattg tttatgatac gatatgtaaa
       61 tgataatcat ccaggaggtc tagcatgtct cgttcaaaaa aatactttta cttatctagc
      121 ttaatgatta ttttaagctt tttctttaat acaaataacg ttttcctaag tggatttttt
      181 aattctttta ttaaattaat acttttctgc agtgttatta actcaattgt actaattttg
      241 tctataattt ttgcagatcg ttcaattaaa tcactaaagc ctgatgcaga ttggattaga
      301 attgcgagta aaagtttgcc ttggattatt ctaattgtta ttttagtaca tatcttttca
      361 attgttcgta cattcggttt tatttaaaaa agataaaatg tcaatgtagc gttaatacaa
      421 aacaatacaa tgtatcatgc tataatgagt aaaacaattt gatgacgttg ttgcgtataa
      481 aaaatattag attttcgaaa tcataactat gcatctaatc gctatagtta tacaacaaga
      541 tataacatat aatgaggttt gataatgcat cgacaatttt tgtcgtcgtg ttgccaaaac
      601 ctctttttta aattcaaact acttcttttc gaggtgaacc aaatgcaata tgtatatatt
      661 tttatcggtg gtgctttagg cgctttatta cgttacctca tttcttttct gaatactgac
      721 ggaggttttc caatcggaac actgatagcc aatttgactg gtgcctttgt aatgggattg
      781 ctaacagcct taacaattgc atttttttca aaccatccga ccctaaaaaa agctattacg
      841 actggttttc ttggtgcttt aacgactttt tcaacatttc aattagaatt aatacatatg
      901 tttgatcatc aacaatttat aactttacta ctatatgctg taacaagtta tgtctttggt
      961 attttgttat gttacgtcgg tataaaacta ggtggtggtt tatcatgata tcaatcattt
     1021 tagtcatgat tggcggcggt tttggcgcaa ttgctagaag tgccattact gattatttta
     1081 atcataaatt tacttcaaag ttacctatcg caacattgat agtaaatcta gttggtagtt
     1141 ttttaattgg attaactata ggcctatcaa tttcaatctc atggttccct gcgttctttg
     1201 tcaccggttt tttaggtggc ttaacaactt tctcaacgtt agccaaggaa cttacattaa
     1261 tgatgacacc aaaatttgat attaaccttt ttctcaatta ttcactttta caattcatca
     1321 ttggatttat agcttgttat attggctatc atatttaaaa ataaaatgct tcattcagca
     1381 aataggtaaa ttacgacacc ttcctgaacg aagcattttt taattttcat gcaaattttt
     1441 taacaccata taatgcctat caaaataaaa caatctttgt tgccgtttaa ataatgtgaa
     1501 tgtcaataaa ttctccaaac tagtcgaaaa taaaacaggc tgggacataa atccctaaaa
     1561 aaacagcagt aagataattt tcaattagaa aatatcttac tgctgttctc tatttataca
     1621 atacttcgta ttgaatggct tcgctttcct agggtgccgt ctcagcctcg gtcttcgact
     1681 ggcactgctc cctcaggagt ctcgccatta atactacgta ttaacatgta attttacttt
     1741 tacatacttt aaaaaaataa gacactttgc ccaacttaca ctaccaatag aaacttctgt
     1801 tagaattcct taaaatgata tttcgcgata tgtaaatgaa attgttgaaa cgatacctga
     1861 tagcgaattc gatgaattca gacatcatcg tggcgcaaca tcctatcatc caaaaatgat
     1921 gttaaaaatc atcttatatg catatactca atctgtattt tctggtcgta gaatagagaa
     1981 attacttcat gacagtattc gaatgatgtg gttagctcaa gatcaaacac cttcttataa
     2041 aactattaat cgttttagag tgaatcctaa tactgatgcg ttaattgaat ctttatttat
     2101 tcagttccat agtcaatgtt taaagcaaaa tcttattgat aataattcaa tttttattga
     2161 tggtacataa gtagaagcta atgccaatag atatacattt gtgtggaaga aaagtattca
     2221 aaatcacgag aagaaataga tttaattggt agtcatttag ataaagaaat cgaagattta
     2281 aatcattcta tttagaacga agattgtgct caaattagaa aacagacccg taaaaaagaa
     2341 ctgagattaa gaagttcaaa aagaaatttg atgattattc cgaaagaaaa aataaatatg
     2401 aagaacaaaa atcgattctt aaagatagaa atagcttttc taaaactgat catgatgcaa
     2461 cttttatgag aatgaaggaa gaccatatga aaaatggcca acttaagcca ggatacaatt
     2521 tacaaatagc gacaaattct caattttttt atcctatgac ctatttcaaa ccccaacaga
     2581 tactagaact ttaattccat ttttaacaat gattcaaaat accttcggtt atttaccaga
     2641 gtatattgta gctgatgcag gttatgatag tgagcaaaac tatatggcta ttatagatga
     2701 ttttaataaa acgccactta ttacgtatgg catgtttatt aaagataaaa ctagaaagtt
     2761 taaaagtgac atttttaaca ctcaaaattg gaaatatgac gaacttaatg atgaatttat
     2821 atgtcctaat aacaaaagaa taggttttaa aagatatgca taccgtaatg atagatatgg
     2881 ttttaaacgt gacttcaagc tatatgaatg cgatgactgt tcagcatgtt ctttgaggca
     2941 acaatgcatg aaaccaaatt cgaaatccaa taagaaaatc atgaagaatt ataattggga
     3001 atactttaaa gcccaaatta atcaaaagct ttctgaacca gaaacgaaaa aaatctatag
     3061 tcaaagaaaa attgatgtag agcttgtttt tggatttatg aaggctattt tgggtttcac
     3121 tcgaatgtca gttcgaggaa taaataa
//
LOCUS       DAHHSC010000234         3127 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.238, whole genome shotgun sequence.
ACCESSION   DAHHSC010000234 DAHHSC010000000
VERSION     DAHHSC010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3127)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3127)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3127
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.238"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            complement(<1..689)
                     /locus_tag="NBI57_002425"
     CDS             complement(<1..689)
                     /locus_tag="NBI57_002425"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HCD1823349.1"
                     /translation="MSKYSQDVLQLLYKNKPNYISGQSIAESLNISRTAVKKVIDQLK
                     LEGCKIDSVNHKGHLLQQLPDIWYQGIIDQYTKSSALFDFSEVYDSIDSTQLAAKKSL
                     VGNQSSFFILSDEQTKGRGRFNRHWSSSKGQGLWMSVVLRPNVAFSMISKFNLFIALG
                     IRDAIQHFSQDEVKVKWPNDIYIDNGKVCGFLTEMVANNDGIEAIICGIGINLTQQLE
                     NFDESIRHRAT"
     gene            complement(676..1878)
                     /locus_tag="NBI57_002426"
     CDS             complement(676..1878)
                     /locus_tag="NBI57_002426"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499992.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HCD1823350.1"
                     /translation="MDKSLFEQARPILEQIQDNGFEAYYVGGSVRDYVMGRNIHDIDI
                     TTSATPDEIESIFSHTIPVGKEHGTINVVFNDENYEVTTFRAEEDYVDHRRPSGVTFV
                     RDLYEDLQRRDFTMNAIAMDTAYKLYDYFDGQQDINNRIIRTVGIAEERFQEDALRMI
                     RCLRFQSQLSFDIAMETFEAMRTQMADIKFLSIERIVIELTKLMRGINVEESFNHLKS
                     LKAFNYMPYFEQLDMNQINVTEPIDLELLIAIVSVKFDINYSLKPLKLSNRQVKDINQ
                     YIQIMNALPSIITKEQLKMFVYDYDTNLIKNVMVAADVLKANDIQGHEPLIVNLQTID
                     ETLHRLPMHNRKDMMVNGGVLMAHLNAKSGPWLKDVLRQIEIAIVTGKVSNEETEILK
                     WVDNHVKI"
     gene            complement(1883..3025)
                     /gene="bshA"
                     /locus_tag="NBI57_002427"
     CDS             complement(1883..3025)
                     /gene="bshA"
                     /locus_tag="NBI57_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-alpha-D-glucosaminyl L-malate synthase
                     BshA"
                     /protein_id="HCD1823351.1"
                     /translation="MKIGITCYPSMGGSGIIATELGIKLAERGHEVHFITSNIPFRIR
                     KPLPNMIFHQVEVNQYAVFQYPPYDITLSTKIAEVIKEYDLDLLHMHYAVPHAICGIL
                     AREMSGKDIKIMTTLHGTDITVLGYDHSLQGAIKFGIEKSDIVTSVSKSLAQETHEII
                     ETNKEIIPIYNFVRENEFPTKHNTALKSQFGIAPDEKVLIHVSNFRQVKRIDTIIETF
                     AKVREKIPSKLILLGDGPELVPMRQLTKELNVEEDVLFLGKQDCVSEFYQLSDLVLLL
                     SEKESFGLTLLEAMKTGVVPIGSNAGGIKEVIKHGETGFVVDVGDCDSASDYAIRLLE
                     DKALYNKLQKNMLADIAERFGSELITDQYEYYYQKMLNEHNKSKGE"
BASE COUNT          956 a          600 c          405 g         1166 t
ORIGIN      
        1 cttgttgctc tatgtctaat actttcatca aagttttcta gttgttgcgt caaattaata
       61 cctataccac atattattgc ttctatacca tcattattag caaccatttc agttaagaaa
      121 ccacacactt taccattatc aatatatata tcattcggcc atttcacttt gacttcatct
      181 tgactaaaat gttgaatcgc atctcttatc cctaatgcaa taaataaatt aaatttagat
      241 atcattgaga atgcaacgtt aggtcttaac acgacagaca tccaaagtcc ttgccctttt
      301 gaagaactcc aatgtctatt aaatcgccca cgacctttcg tttgttcatc actcaagata
      361 aaaaatgaag attgatttcc aacaagtgac tttttcgcag caagttgtgt agaatctatt
      421 gaatcgtata cttcactaaa atcaaacaaa gcagaacttt ttgtatattg gtctattata
      481 ccctgatacc aaatatctgg gagttgttgt aataaatgcc ctttatgatt tactgaatca
      541 attttacatc cctctaactt taattggtca atcacctttt ttactgcagt gcgtgaaata
      601 ttaagtgatt ccgcaatgct ttgtccagat atataattcg gtttattttt atagagtaat
      661 tgaagtacat cttgactata ttttgacatg attatccacc catttcaaaa tttcagtttc
      721 ttcgttgctt actttacctg ttacaatcgc tatctcaatt tgtcttagca catcttttaa
      781 ccacggacca cttttggcat ttaaatgtgc cataagtaca ccgccattaa ccatcatgtc
      841 ttttctatta tgcataggta aacgatgtaa tgtttcatca atcgtttgaa ggttaacgat
      901 taatggttca tgtccttgaa tatcatttgc ctttaacacg tctgctgcaa ccattacatt
      961 tttaatgaga ttcgtatcat aatcataaac aaacattttt aattgttctt ttgtaataat
     1021 acttggtaat gcattcataa tttgaatata ttgattgata tcttttactt gtcggttact
     1081 tagctttaaa ggcttcaatg agtaattaat atcaaattta actgacacta tagcaatcaa
     1141 caattctaaa tcaattggtt cagttacatt aatttgattc atatcaagtt gttcgaaata
     1201 tggcatataa ttaaatgctt tcagcgattt taaatgatta aaactctctt caacattaat
     1261 acctcgcatt aatttagtta gttcaatcac tatacgctca attgataaaa atttaatatc
     1321 tgccatttgt gtacgcatcg cttcaaatgt ttccattgca atatcaaatg ataattgtga
     1381 ctggaatctt aaacatcgaa tcatacgtaa agcatcttct tggaatcgtt cctcagctat
     1441 acctacagtt cttattattc gattattaat atcttgttga ccatcaaaat aatcatacaa
     1501 tttgtatgct gtatccattg ctatcgcatt catcgtgaaa tctcttcgtt gcaaatcttc
     1561 gtataaatcc cgaacaaatg taacaccact tggtctacgg tgatcgacat aatcttcttc
     1621 agcccggaat gttgtcactt cataattttc atcattaaaa actacattta tggtgccatg
     1681 ttctttacct acaggtatcg tatgactaaa gatagattct atttcatccg gcgttgcact
     1741 tgttgtgata tctatatcat gaatatttct tcccatgaca taatctctta cagagccacc
     1801 tacataatat gcttcaaaac cattgtcttg aatttgttct aatataggcc ttgcctgttc
     1861 aaataatgat ttatccatat tattactcgc ctttactttt gttatgctca tttagcattt
     1921 tttgataata atactcatat tgatctgtaa taagttctga tccaaaacgt tcagcaatat
     1981 ctgctagcat gtttttctga agtttgttgt ataacgcctt atcttcaagt aaacggatag
     2041 catagtcact cgctgaatca caatcaccca catctacaac aaatccagtt tcaccatgtt
     2101 taataacctc tttaattcca ccggcatttg aaccaattgg aacgacgcct gttttcatag
     2161 cctcaagtaa agttagtcca aagctttctt tttcacttaa taataatact aagtcagata
     2221 attgataaaa ttcacttaca caatcttgtt tccctaaaaa taaaacatcc tcttctacgt
     2281 ttaactcttt cgtcaattga cgcattggca ctaattcagg accatctcca agtaaaatta
     2341 atttactagg tatcttttca cgtacttttg caaatgtttc tataatagta tctatgcgtt
     2401 ttacttgtct aaaattcgat acatgtatta acactttttc atctggtgct ataccaaatt
     2461 gtgattttaa tgctgtgtta tgtttagttg gaaactcatt ttcacgtaca aaattataaa
     2521 tcggtataat ttctttgtta gtttcgataa tttcatgtgt ttcttgcgct aaagatttac
     2581 tcacacttgt cacaatatca cttttttcaa tgccaaattt aattgcacct tggagtgaat
     2641 gatcatagcc caaaacagta atatcagtac cgtgtagcgt tgtcataatt tttatatctt
     2701 tacctgacat ctcacgagct aaaattccac aaattgcatg aggtacagca tagtgcatat
     2761 gcaacaaatc aagatcatat tctttaataa cttcagcgat tttagtactt aacgtaatat
     2821 catacggtgg atattgaaat actgcatatt gattcacttc aacttgatga aaaatcatat
     2881 tcggcaatgg ttttcttatt ctaaacggga tatttgaagt gataaaatgt acttcgtgac
     2941 ctcgctctgc taatttaatt cctaattctg tggcaataat tccagaacca cccatggacg
     3001 ggtaacatgt tatacctatc ttcattcgct tgcccatcct ttctttctat ttctctttct
     3061 attattctcg atgcgtagat aattgtttaa atttaagttt atagtaatgt tgagtttata
     3121 atttcaa
//
LOCUS       DAHHSC010000235         3100 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.239, whole genome shotgun sequence.
ACCESSION   DAHHSC010000235 DAHHSC010000000
VERSION     DAHHSC010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3100)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3100)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3100
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.239"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            74..2977
                     /gene="sdrC"
                     /locus_tag="NBI57_002428"
     CDS             74..2977
                     /gene="sdrC"
                     /locus_tag="NBI57_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MSCRAMM family adhesin SdrC"
                     /protein_id="HCD1823352.1"
                     /translation="MNNKKTATNRKGMIPNRLNKFSIRKYSVGTASILVGTTLIFGLS
                     GHEAKAAEHTNGELNQSKNETTAPSENKTTEKVDSRQLKDNTQTATADQPKVTMSDSA
                     TVKETSSNMQSPQNATASQSTTQTSNVTTNDKSSTTYSNETDKSNLTQAKNVSTTPKT
                     TTIKQRALNRMAVNTVAAPQQGTNVNDKVHFTNIDIAIDKGHVNKTTGNTEFWATSSD
                     VLKLKANYTIDDSVKEGDTFTFKYGQYFRPGSVRLPSQTQNLYNAQGNIIAKGIYDSK
                     TNTTTYTFTNYVDQYTNVSGSFEQVAFAKRENATTDKTAYKMEVTLGNDTYSKDVIVD
                     YGNQKGQQLISSTNYINNEDLSRNMTVYVNQPKKTYTKETFVTNLTGYKFNPDAKNFK
                     IYEVTDQNQFVDSFTPDTSKLKDVTGQFDVIYSNDNKTATVDLLNGQSSSDKQYIIQQ
                     VAYPDNSSTDNGKIDYTLETQNGKSSWSNSYSNVNGSSTANGDQKKYNLGDYVWEDTN
                     KDGKQDANEKGIKGVYVILKDSNGKELDRTTTDENGKYQFTGLSNGTYSVEFSTPAGY
                     TPTTANAGTDDAVDSDGLTTTGVIKDADNMTLDSGFYKTPKYSLGDYVWYDSNKDGKQ
                     DSTEKGIKGVKVTLQNEKGEVIGTTETDENGKYRFDNLDSGKYKVIFEKPAGLTQTGT
                     NTTEDDKDADGGEVDVTITDHDDFTLDNGYYEEETSDSDSDSDSDSDSDSDSDSDSDS
                     DSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSESDSDSDSDSDSDSDSDSDSDSDSDS
                     DSDSDSDSDSESDSDSDSDSDSDSDSDNDSDSDSDSDSDSDSDSDSDSDSDSDSDSDS
                     DSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDAGKHTPT
                     KPMSTVKDQHKTAKALPETGSENNNSNNGTLFGGLFAALGSLLLFGRRKKQNK"
BASE COUNT         1219 a          499 c          575 g          807 t
ORIGIN      
        1 tagtaatgca gaaatttata tatgcaaaat attatattta tcaaattttg atattttaaa
       61 ggagtattat taaatgaata ataaaaagac agcaacaaat agaaaaggca tgataccaaa
      121 tcgattaaac aaattttcga taagaaagta ttctgtaggt actgcttcaa ttttagtagg
      181 gacaacattg atttttgggt taagtggtca tgaagctaaa gcggcagaac atacgaatgg
      241 agaattaaat caatcaaaaa atgaaacgac agccccaagt gagaataaaa caactgaaaa
      301 agttgatagt cgtcaactaa aagacaatac gcaaactgca actgcagatc agcctaaagt
      361 gacaatgagt gatagtgcaa cagttaaaga aactagtagt aacatgcaat caccacaaaa
      421 cgctacagct agtcaatcta ctacacaaac tagcaatgta acaacaaatg ataaatcatc
      481 aactacatat agtaatgaaa ctgataaaag taatttaaca caagcaaaaa acgtttcaac
      541 tacacctaaa acaacgacta ttaaacaaag agctttaaat cgcatggcag tgaatactgt
      601 tgcagctcca caacaaggaa caaatgttaa tgataaagta cattttacga acattgatat
      661 tgcgattgat aaaggacatg ttaataaaac aacaggaaat actgaatttt gggcaacttc
      721 aagtgatgtt ttaaaattaa aagcgaatta cacaatcgat gattctgtta aagagggcga
      781 tacatttact tttaaatatg gtcaatattt ccgtccaggt tctgtaagat taccttcaca
      841 aactcaaaat ttatataatg cccaaggtaa tattattgca aaaggtattt acgatagtaa
      901 aacaaataca acaacgtata cttttacgaa ttatgtagat caatacacaa atgttagcgg
      961 tagctttgaa caagtcgcat ttgcgaaacg tgaaaatgca acaactgata aaactgctta
     1021 taaaatggaa gtaactttag gtaatgatac atatagtaaa gatgtcattg tcgattatgg
     1081 taatcaaaaa ggtcaacaac ttatttcgag tacaaattat attaataatg aagatttgtc
     1141 acgtaatatg actgtttatg taaatcaacc taaaaagacc tatacaaaag aaacatttgt
     1201 aacaaattta actggttata aatttaatcc agatgctaaa aacttcaaaa tttacgaagt
     1261 gacagatcaa aatcaatttg tggatagttt caccccagat acttcaaaac ttaaagatgt
     1321 tactggtcaa ttcgatgtta tttatagtaa tgataataag acggcgacag tagatttatt
     1381 gaatggtcaa tctagtagtg ataaacagta catcattcaa caagttgctt atccagataa
     1441 tagttcaaca gataatggga aaattgatta tactttagaa acacaaaatg gaaaaagtag
     1501 ttggtcaaac agttattcaa atgtgaatgg ctcatcaact gcaaatggcg accaaaagaa
     1561 atataatcta ggtgactatg tatgggaaga tacaaataaa gatggtaaac aagatgccaa
     1621 tgaaaaaggg attaaaggtg tttatgtcat tcttaaagat agtaacggta aagaattaga
     1681 tcgtacgaca acagatgaaa atggtaaata tcagttcact ggtttaagca atggaactta
     1741 tagtgtagag ttttcaacac cagccggtta tacaccgaca actgcaaatg caggtacaga
     1801 tgatgctgta gattctgatg gactaactac aacaggtgtc attaaagacg ctgacaacat
     1861 gacattagat agtggattct acaaaacacc aaaatatagt ttaggtgatt atgtttggta
     1921 cgacagtaat aaagatggta aacaagattc gactgaaaaa ggaattaaag gtgttaaagt
     1981 tactttgcaa aacgaaaaag gcgaagtaat tggtacaact gaaacagatg aaaatggtaa
     2041 ataccgcttt gataatttag atagtggtaa atacaaagtt atctttgaaa agcctgctgg
     2101 tttaactcaa acaggtacaa atacaactga agatgataaa gatgccgatg gtggcgaagt
     2161 tgatgtaaca attacggatc atgatgattt cacacttgat aatggctact acgaagaaga
     2221 aacatcagat agtgactcag attcggacag cgattcagac tcagatagcg actcagattc
     2281 agatagtgac tcagactcag atagcgactc agactcagat agcgactcag acagcgactc
     2341 agactcagat agtgattcag attcggacag cgactcagat tcagacagcg aatcagattc
     2401 ggatagcgac tcagactcag atagcgactc agattcagac agcgactcag attcagacag
     2461 tgactcagac tcagacagcg actcagattc agacagcgaa tcagattcgg atagcgactc
     2521 agattcagat agcgattcgg actcagacaa cgactcagat tctgacagcg attcagactc
     2581 agatagcgac tcagattcag acagcgactc agactcagat agcgattcag attcagacag
     2641 cgactcagat tcagacagcg attcagattc agatagcgat tcagattcag acagcgactc
     2701 agattcagat agcgactcag actcagacag cgattcagac tcagatagcg actcagacag
     2761 cgattcagat tcggatagcg attcagattc agatgcaggt aaacatactc cgactaaacc
     2821 aatgagtacg gttaaagatc agcataaaac agctaaagca ttaccagaaa caggtagtga
     2881 aaataataat tcaaataatg gcacattatt cggtggatta ttcgcggcat taggatcatt
     2941 attgttattc ggtcgtcgta aaaaacaaaa taaataatat gattaactta accaggtcca
     3001 tgtggcctgg ttttttctgt ttagaaattc ccagatataa aacaaaaaat ctttaaggaa
     3061 tagatgcaat ttaatttgtt acagaaatgt aattgtctat
//
LOCUS       DAHHSC010000236         3094 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.240, whole genome shotgun sequence.
ACCESSION   DAHHSC010000236 DAHHSC010000000
VERSION     DAHHSC010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3094)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3094)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3094
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.240"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            88..705
                     /gene="cadD"
                     /locus_tag="NBI57_002429"
     CDS             88..705
                     /gene="cadD"
                     /locus_tag="NBI57_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017465246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium resistance transporter CadD"
                     /protein_id="HCD1823353.1"
                     /translation="MIQTVVAAAVLYIATAVDLLVILLIFFARAKTRKEYRDIYVGQY
                     LGSIILILVSLFLAFVLNYVPEKWILGLLGLIPIYLGIKVAIYDDCEGEKRAKKELNE
                     KGLSKLVGTVAIVTIASCGADNIGLFVPYFVTLSVTNLLLTLFVFLILIFFLVFTAQK
                     LANIPGIGEIVEKFSRWIMAIIYIALGLFIIIENDTIQTILGFIF"
     gene            724..1071
                     /locus_tag="NBI57_002430"
     CDS             724..1071
                     /locus_tag="NBI57_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020366422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCD1823354.1"
                     /translation="MSYENTCDVICVHEDKVNNALSFLEDDKSKKLLNILEKICDEKK
                     LKIILSLIKEDELCVCDISLILKMSVASTSHHLRLLYKNEVLDFYKDGKMAYYFIKDD
                     EIREFFSKNHEGF"
     gene            1253..1393
                     /locus_tag="NBI57_002431"
     CDS             1253..1393
                     /locus_tag="NBI57_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HCD1823355.1"
                     /translation="MQYNTTRSITENQDNKTLKDMTKSGKQRPWREKKIDNVRFCCKV
                     KK"
     gene            complement(1428..1529)
                     /locus_tag="NBI57_002432"
                     /pseudo
     CDS             complement(1428..1529)
                     /locus_tag="NBI57_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105942.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
BASE COUNT         1127 a          356 c          477 g         1134 t
ORIGIN      
        1 caataaagca attggaataa agcacctatg attatttaaa aatataagtt ataatacatt
       61 caaaaatatt tttgaatggg gtgtattatg attcaaacgg ttgttgctgc tgctgttctt
      121 tatattgcta cagcagttga tttattagta attttattaa tattttttgc tagagcaaag
      181 actagaaaag aatatagaga tatttatgtt ggtcaatatt tagggtctat tattttaata
      241 ttagttagtt tgtttttagc ttttgtatta aattatgttc cagaaaagtg gatattaggt
      301 ttattgggtt taataccaat ttatcttgga attaaagtgg ctatttatga tgattgtgaa
      361 ggagaaaaga gagctaaaaa agaattgaat gaaaaaggat tgtctaaatt agttggtacg
      421 gttgcaattg ttacgatagc aagttgtggc gccgataata ttggtttatt tgttccgtat
      481 tttgtgacat taagtgttac taatttatta cttactctgt ttgtcttttt aattttaatt
      541 ttcttcttgg tgtttactgc acaaaaatta gctaatatcc caggaattgg agaaattgtt
      601 gagaaattta gtcgttggat tatggctatc atttatatag ctttaggttt atttattatt
      661 attgaaaatg acactattca aacaatttta ggatttatat tttaatttag ggtgtgattt
      721 tatatgagtt atgaaaatac ttgtgatgtg atctgtgtac atgaggataa agttaacaat
      781 gctttaagtt ttttagaaga tgataaatct aagaaattac ttaacatttt agaaaaaatt
      841 tgtgatgaga agaaattgaa aatcatatta tctttgatta aagaagatga gttgtgtgtt
      901 tgtgatattt ctttgatatt gaaaatgagt gttgcttcaa cttcacatca tttaaggctt
      961 ttatataaaa atgaggtact tgatttttat aaagatggaa agatggcata ttattttatt
     1021 aaagacgatg aaataagaga gtttttctct aaaaatcatg agggtttttg aaacgagttt
     1081 cttcttgtct tgatactata tagaaataac gctcttttct aaaatccttt tgaagcgttg
     1141 atatgacggc gtttcatact gttttgcttg gggaaaataa aatttgatat aaaaaaactc
     1201 cagtaatatg attgaggtgt ctaatctaaa tcaatactgg agttcttttt atatgcaata
     1261 taatactact agaagtataa ccgaaaatca agataataaa acgttaaaag atatgacgaa
     1321 aagtgggaaa caacgcccat ggagagaaaa gaaaatagat aatgtaaggt tctgttgcaa
     1381 agttaaaaaa taagtgtcta accttatgtt taaagtaata ctttgtatta actagctagc
     1441 atgatgctaa ttacacggca tggcgaaaac ccgtagatct gaagagatct gcggttcttt
     1501 ttatatagtt cataaataca ttcaatgccc gtgtatttta ttccgattgc tttattgtcg
     1561 ttgagcctcg gaacccttaa caatcccaaa acttgtcgaa tggtcggctt aatagctcac
     1621 gctatgccga cattcgtctc caagtttagt taaggcttct tctcaacgca caataaattt
     1681 tctcggcata aatgcgtcgt ctaacttttt ttataatgtt tatcaaacca cgatgaaaaa
     1741 aatataaaaa agactgtata aagaataaac caagtatcta aatgattttg attcatttgc
     1801 ttataaataa aaaacaataa aaaaatacca agaataattt gtaaaataaa taaagacctc
     1861 ttcaaaaaac cactcctttt aaaaaacttt atctcaggac ttattcaaat tataaacacc
     1921 tttattttga attttaagag ggtattttaa agatttagag gtaaatctta tagttttcta
     1981 cttaaaaatc tatacaagct cttaaaacgc aaatatgagc caaataaata tatggtaatt
     2041 tcacaaacaa aaatttcagc aaaaccagtg actaattttt taacactgcc catttaaaat
     2101 gcaaattcgg aacgatttat tgctaatttt taaatttgca tgtaaatggg cagtacgtaa
     2161 aaatcgcccg tcctggtgtc gaaaaaacga acagaaaaaa taaaattaaa atatagtaat
     2221 ctttttattt tttacaggta ggtttcccca taatttagta ctgcatcaga aatttatctg
     2281 tacttagaaa tgacgacgta ttttttctat acgtttatga catttattct acattgatcc
     2341 aaatcataaa aatgtgttta aaatgtccta aaaaggtttt aaaaggacga tttttttata
     2401 ttacgtattt ttgtactcaa aattagagaa tgctctaaat gacctcttaa aacgcaaaaa
     2461 aaaatatata tcaaaaagac atgtattttt aaaaaaattg atgtgtatga tgcatgtctt
     2521 ttgtgtatgt tttatataca taaagtatat gtatacgtaa cccgtatgta tacataacgt
     2581 tatggttttt atacatatga gttaaaaaag tgatgtcact ttttttctag gaaaaataac
     2641 tcggcactgc cgagttcacc acgttatggg tctgccaaaa tataaaagtg gtggcacttt
     2701 cccttatgct catgacttta tgtataaaag ttagaggtac ttttatgccg agaaaacttt
     2761 ttgagagatt aattttttta tttttttatt ttttactttt attttcttca aatattttgt
     2821 aaatctcttt atgagtaaat aaaagataga agcataaaga ataaatgaaa atttgaaaat
     2881 ataaatttat atgaatgttg tagaaaatct tcaaaattat taagtttaat acgatcatat
     2941 tagcagttat aaagaatatg aataagataa tattattaaa tttctttttg tattttatat
     3001 gtaaaaatat tataaaaagt tcaagaaaaa taaatattag ataagttact ataaagaata
     3061 atgcaatata gccaatgtta taaaacatat tatt
//
LOCUS       DAHHSC010000237         3080 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.241, whole genome shotgun sequence.
ACCESSION   DAHHSC010000237 DAHHSC010000000
VERSION     DAHHSC010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3080)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3080)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3080
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.241"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            complement(309..971)
                     /locus_tag="NBI57_002433"
                     /pseudo
     CDS             complement(309..971)
                     /locus_tag="NBI57_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500433.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
     gene            complement(964..2520)
                     /locus_tag="NBI57_002434"
     CDS             complement(964..2520)
                     /locus_tag="NBI57_002434"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000028656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HCD1823356.1"
                     /translation="MSITEKQRQQQAELHKKLWSIANDLRGNMDASEFRNYILGLIFY
                     RFLSEKAEQEYADALSGEDITYQEAWADEEYREDLKAELIDQVGYFIEPQDLFSAMIR
                     EIETQDFDIEHLATAIRKVETSTLGEESENDFIGLFSDMDLSSTRLGNNVKERTALIS
                     KVMVNLDDLPFVHSDMEIDMLGDAYEFLIGRFAATAGKKAGEFYTPQQVSKILAKIVT
                     DGKDKLRHVYDPTCGSGSLLLRVGKETQVYRYFGQERNNTTYNLARMNMLLHDVRYEN
                     FEIRNDDTLENPAFLGNTFDAVIANPPYSAKWTADSKFENDERFSGYGKLAPKSKADF
                     AFIQHMVHYLDDEGTMAVVLPHGVLFRGAAEGVIRRYLIEEKNYLEAVIGLPANIFYG
                     TSIPTCILVFKKCRQQDDNVLFIDASNDFEKGKNQNHLSDAQVERIIDTYKRKATIDK
                     YSYSATLQEIADNDYNLNIPRYVDTFEEEAPIDLDQVQQDLKNIDKEIAEIEQEINAY
                     LKELGVLKDE"
     gene            complement(2684..2818)
                     /locus_tag="NBI57_002435"
     CDS             complement(2684..2818)
                     /locus_tag="NBI57_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1823357.1"
                     /translation="MHYLICIKANQRLIFTNSIVDWFYLCMNEQLFDIIKNINDFESI
                     "
     gene            complement(2881..>3080)
                     /gene="splF"
                     /locus_tag="NBI57_002436"
     CDS             complement(2881..>3080)
                     /gene="splF"
                     /locus_tag="NBI57_002436"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="serine protease SplF"
                     /protein_id="HCD1823358.1"
                     /translation="SVNGNIVSSDAIIQPGSSGSPILNSKHEAIGVIYAGNKPSGEST
                     RGFAVYFSPEIKKFIADNLDK"
BASE COUNT          859 a          566 c          488 g         1167 t
ORIGIN      
        1 tctatccctt taaagacagg gtaatattta ctaataatac ctacatcaat cattagattt
       61 aaattaaact taaaaattcc atcgtcgctt cttgaacggt atgttatatg atttttaggg
      121 agtatgaaat acccaatatt actctctgca aaagttttcc tatctttata gtagtctgac
      181 tgaagtatta aaccttgtct tgacgatgtt aaaacaggat attcattttc attaatagtt
      241 cttttattac taaattctaa tacatctttt aattgctttt tgttccatcc tttatagtaa
      301 ttaccttctt catctttgaa tcgtaattct tgtgagaaga ttttctgtat atagcatttt
      361 ttctgttgtt gaagtaattc aagtttttgt tcttctaatt caatttgttg gtcaagtttg
      421 ctgaagaatt gtcctatttt ttgttgttct tcaaatatag ttggggtgaa gatttttaaa
      481 ttagcaattt ctttgaagtt tagtccttct cgactacctc cactttgtgc aaggaaaatt
      541 tttcttttac cttttcttga taatagatac tgtccaaaaa aattataata atactctttt
      601 tttaatctaa taatacatac atgttgattt aaattagcat gcgtttcaac tatcgaatta
      661 atggctgttc tacctattga tgctcctgta atatttaaaa gaacatcacc atagtacgtt
      721 ctactatttt tcatctcatc atctatatct ttactaatat aaactaagtc attaagattt
      781 aatttaccat ttctaatatt ttgactcctt aaaaatggta tgcctttgtt tgtatagttt
      841 tcacttccac ctttgggagt ctttccacta cctattttgg tagtaagatt ccctaacttc
      901 ttctcttccc attcgccctc aaatcctggg aatctcaact ctggcacatt tttcgtttgt
      961 gtattactca tctttcaaca ccccaagttc tttcaggtat gcattgattt cttgttcaat
     1021 ttctgcgatt tctttgtcga tatttttcaa atcttgttgg acttgatcta aatcaattgg
     1081 tgcttcttct tcgaatgtat caacatatcg cggtatgttt aagttgtaat cgttatcggc
     1141 gatctcttgt aatgtcgcgc tgtagctata tttatcaatc gttgctttac gcttatatgt
     1201 gtctataata cgttcgactt gggcatcgct taaatggttt tgattttttc ctttttcaaa
     1261 atcattggat gcatcgataa atagtacgtt gtcgtcttgt tggcgacatt ttttaaatac
     1321 taaaatacat gttggaatac ttgtcccata gaaaatattg gctggtaacc caatcacggc
     1381 ttctaagtag ttcttttctt ctattaaata gcgacgaatc acaccttctg cggcaccacg
     1441 gaataataca ccatgtggga gtacgactgc catggtacct tcatcgtcta ggtaatgtac
     1501 catgtgttga ataaaggcaa agtctgcttt ggattttggc gcaagcttgc cgtaaccact
     1561 gaatcgttca tcattttcaa attttgaatc tgctgtccat ttcgcactgt atggtgggtt
     1621 cgcaataacc gcatcaaatg tattgcctaa aaaggctgga ttttccaatg tgtcatcatt
     1681 acggatctcg aagttctcat aacgcacatc atgtaataac atattcatgc gtgctaagtt
     1741 gtatgtagta ttgttacgtt cttgtccgaa ataacgatac acttgcgttt ctttaccaac
     1801 acgtaacaac aatgaaccgg aaccacatgt tgggtcgtac acgtgacgta atttatcttt
     1861 accgtctgtg acaatcttcg ccagtatctt agatacttgt tgtggtgtat agaactcgcc
     1921 tgctttttta cccgctgtcg ccgcaaagcg cccgattagg aattcatatg catcacctaa
     1981 catatcaatt tccatgtcac tgtgaacgaa tggtaagtcg tcaagattaa ccatgacttt
     2041 agagattaaa gcagtacgtt ctttgacatt gttacctagt cgcgttgaac tcaaatccat
     2101 atcgctgaac agcccgataa agtcattttc actttcttca cctaatgtag atgtttcaac
     2161 tttgcgaatc gccgtcgcta gatgttctat atcgaaatct tgcgtttcaa tttcacgaat
     2221 catcgcactg aataaatctt gtggctcaat gaagtaaccg acttgatcaa ttaattctgc
     2281 ttttaagtct tcacgatatt cttcatctgc ccatgcttct tgatacgtga tgtcttcacc
     2341 tgacaaggca tctgcatatt cttgttcggc tttttcagat aagaagcgat agaaaatcaa
     2401 gcctaaaatg taattacgga attcactcgc atccatgttc cctcttaaat cattcgcaat
     2461 cgaccataat tttttatgta attcagcttg ttgctgacgt tgtttttcag taatagacat
     2521 gtgattcctc cgcctttgca taagtaattt atctctttgt gtaatagatt tattataaca
     2581 tttggttatg ttgcgatgtt gataatttgg atgttggtgg tggaaatttt gagttttagt
     2641 ggcgcaattg ttattgaaaa attttataga aatgttgtag ctttcaaatg ctttcaaaat
     2701 catttatatt cttaatgatg tcaaaaagtt gttcattcat acataaataa aaccaatcaa
     2761 caattgagtt ggtgaaaatc aatcgttgat tggctttgat gcatattaaa taatgcaata
     2821 tattaataaa tgttagttat agtatatttt gtcaggattg ggtgaatgtc taagttttaa
     2881 ttatttatct aaattatctg caatgaattt cttaatttca ggagagaaat aaacagcaaa
     2941 tcctcttgtg ctttcacctg atggcttatt accggcatag attacaccaa tagcttcgtg
     3001 tttactattt aatataggtg aaccagagct accaggctga ataattgcat ccgatgacac
     3061 tatattccca ttcactgata
//
LOCUS       DAHHSC010000238         3042 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.242, whole genome shotgun sequence.
ACCESSION   DAHHSC010000238 DAHHSC010000000
VERSION     DAHHSC010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3042)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3042)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3042
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.242"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            <1..730
                     /locus_tag="NBI57_002437"
     CDS             <1..730
                     /locus_tag="NBI57_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001585007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tandem-type lipoprotein"
                     /protein_id="HCD1823359.1"
                     /translation="CIIMTKINSRNAQIKDTFNQTLNVYPTKNLDDFYDKEGFRDQEF
                     DKRDKGTWIINSGMYIQLKGGALKSRAMVLYINRNTRTAKGYFLISETTEDKKGYVHN
                     KDKKYPVKMERNRIIPTKPITDEKLKKEIENFKFFVQYGNFKDFKDYKDGDISYNPNV
                     PSYSAKYQLNNDDYNVQQLRKRYDISTKRAPELKLRGSGDLKGSSVGSKELEFNFVRN
                     KEENVYFSDGINFKPTEEMNHEQN"
     gene            717..2042
                     /locus_tag="NBI57_002438"
     CDS             717..2042
                     /locus_tag="NBI57_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001619610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1823360.1"
                     /translation="MNKTKYQINSDNIKSNSEETSAISSISYEIENANNNDLNNASIQ
                     TQIEILKSQNSFPKNLSYLKSYTDPKTGTTTSAFLNKDTGKVTLGMTGTNVHKDAILK
                     QTFGVPSYQGYIDASETLKDIGADVNIGLHSVTDKDPHYKNTQDFIKNIKKDYDIDII
                     TGHSLGGRDAMILGMSNDIKHIVVYNPAPLAIKDVSGLYADQEELKKLIEKYDGHIVR
                     FVSDEDELDAGVRNHLYETAGEKIVLKNGEGHAMSGILMSRTQAIILAELNKVKGYQD
                     ENNKALKSVRKQTRHRLHKVETLRANWIQTTGGSLSSSQQQLLEALTALTIAEGLNQL
                     VNEESQHLKKMYHAMAHKFGDNWKKAQEVGNEIGEKLTSEEVIDELRKGGAYESKLET
                     DPKRKIDDKIKKLNDVYKNCNGYIAKIKQSIEAIVSNDQMLASQIDGMM"
     gene            2042..2368
                     /locus_tag="NBI57_002439"
     CDS             2042..2368
                     /locus_tag="NBI57_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1823361.1"
                     /translation="MFTTYKNINELENAYDEERKQLNDAFNQIDELRHQTRKKCEQMY
                     DHFLYLKHKMNYSEDAMIRMTRIIESFDRETNQRIRHHEMKLEDYKDELRREYLKQSD
                     RIEGDE"
     gene            2371..2658
                     /locus_tag="NBI57_002440"
     CDS             2371..2658
                     /locus_tag="NBI57_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1823362.1"
                     /translation="MGLDFSGLPDLAVLEQMKEKEQISEVIAPEHVRMHHDHQNKLKS
                     DEKILLDQMVSHFKNFEDDFKNAAQGAWVKNATDELKDISNDLEKIQDIKV"
BASE COUNT         1250 a          404 c          548 g          840 t
ORIGIN      
        1 ttgtatcatt atgacaaaaa taaatagtcg aaatgcccaa attaaagaca catttaatca
       61 aacattaaat gtgtatccaa ccaagaatct tgatgatttt tacgataagg aaggctttcg
      121 agatcaagaa tttgataaaa gagataaagg gacttggatt attaattctg gaatgtacat
      181 ccaattaaaa ggaggagcct taaaatccag agcgatggta ctttacatca atcgtaacac
      241 tagaacagct aagggatatt ttttaataag tgagacaaca gaagacaaaa aaggatacgt
      301 tcataataaa gataaaaaat accctgtcaa gatggaacgt aatcgaataa ttccaacgaa
      361 gcccataacg gacgagaagt taaaaaaaga gattgaaaac tttaagttct ttgtacaata
      421 tgggaatttt aaagatttta aagattataa agatggggat atttcataca atcctaacgt
      481 accaagttat tccgcaaagt atcaattgaa taatgatgat tataatgtcc aacaattaag
      541 aaaacgatat gatatatcaa ccaaaagggc acctgaatta aaattgagag gatctggcga
      601 tttaaaaggc tcatccgtag gttctaaaga actagaattt aactttgtaa gaaataaaga
      661 ggaaaatgtg tatttttctg atggtattaa tttcaaacct actgaggaaa tgaatcatga
      721 acaaaactaa atatcaaata aattctgaca atataaagag taactctgaa gaaacaagcg
      781 caatatctag tataagttat gaaatagaaa acgcaaataa taacgattta aataatgcaa
      841 gtattcaaac gcaaatagaa atattaaaat cacaaaatag tttccctaaa aatctttcat
      901 atcttaaaag ttatacggac cccaaaacag gtacgactac aagcgccttt ttaaataaag
      961 acactggcaa agttacttta ggtatgacag gtactaatgt acacaaagac gcaatattaa
     1021 aacaaacatt tggtgttcct tcttatcaag gatatataga tgcgagtgaa acgctaaagg
     1081 atattggggc cgatgtcaat attggccttc attccgtcac agataaagat ccacattata
     1141 aaaataccca agactttatc aaaaatatca aaaaagacta tgatattgat attattaccg
     1201 gacattcgct gggcggtaga gatgcgatga ttttaggtat gagtaatgat attaaacata
     1261 tcgttgtgta taatccagct ccattagcga tcaaagatgt gagcggcctt tatgccgatc
     1321 aagaagaact taaaaaattg attgaaaagt acgatggtca cattgtaaga tttgtgtctg
     1381 atgaagacga attagatgca ggtgtccgca atcatttata tgaaactgct ggagaaaaaa
     1441 tagtacttaa aaatggagaa ggccatgcaa tgagtggtat tttaatgagc agaacacagg
     1501 ctataatctt agctgaatta aacaaagtta aaggctacca agacgaaaat aataaagcat
     1561 taaaatccgt tcgtaaacaa acgaggcata gattacataa agtagagacg ttaagagcga
     1621 attggattca aacaacgggt ggatcactct cttcctccca acaacaatta ttagaagctt
     1681 taacagcact aaccattgcc gaaggcttaa atcaattagt gaatgaagaa agccaacatt
     1741 tgaaaaaaat gtatcacgcg atggcacata aatttggaga caactggaaa aaagcgcaag
     1801 aagttggaaa tgaaattggt gaaaaattaa cctctgaaga ggttatagat gaattaagaa
     1861 aaggtggcgc gtatgaaagt aaacttgaaa cagatcccaa aagaaaaatt gatgataaga
     1921 taaagaaatt aaatgatgtt tataaaaatt gtaatggcta tatcgcaaaa attaaacaga
     1981 gtatcgaagc aattgtttct aatgaccaaa tgttagcgag ccagattgat gggatgatgt
     2041 aatgtttact acgtataaaa atattaatga acttgaaaat gcctatgatg aagaaagaaa
     2101 acaattgaat gatgcattca atcaaattga tgaattaaga catcaaacac gcaagaaatg
     2161 tgaacaaatg tatgatcatt tcttatatct caaacataaa atgaattata gtgaagacgc
     2221 tatgatcagg atgacacgta ttatagaatc tttcgataga gaaacgaatc aacgtatccg
     2281 acatcacgaa atgaaattag aagattataa agatgagtta agaagagaat atctaaaaca
     2341 atctgacaga attgaaggag atgaataagc gtgggacttg attttagtgg tttaccagat
     2401 ttagcagtat tggaacaaat gaaggaaaaa gaacagatta gtgaggttat tgcgcctgaa
     2461 catgttcgta tgcatcatga tcatcaaaat aagctgaaaa gtgatgagaa aatattactt
     2521 gaccaaatgg taagtcattt caaaaacttt gaagatgatt ttaaaaatgc ggcacaaggg
     2581 gcttgggtga aaaatgccac agacgaatta aaagatatta gtaatgattt agaaaaaatt
     2641 caagatatta aagtataaaa aggtattaag aaacacaata agtatataat ccatttaata
     2701 ataaataaat tatatagttc ataatctcga ctataagtgg ctattagcca ttatttttcg
     2761 ggatctatgt caaatcggac taatgaattc aataatggaa gttaagcaac caatctttgt
     2821 ttaacttctt ttttattttt tggaaaataa agttttgaac ataataaaat ttgattatgt
     2881 tttaacgaat tttgatgttt cttgaactat atatcatcta gtcgtcattt acaattggta
     2941 aatatgactt caaactgtat gaaggtgatg gcgattaaaa ggctcatccg taggttctaa
     3001 agaactagaa tttaacttta taagaaataa agaagaaaat gt
//
LOCUS       DAHHSC010000239         2939 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.243, whole genome shotgun sequence.
ACCESSION   DAHHSC010000239 DAHHSC010000000
VERSION     DAHHSC010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2939)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2939)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2939
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.243"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            6..2279
                     /gene="recJ"
                     /locus_tag="NBI57_002441"
     CDS             6..2279
                     /gene="recJ"
                     /locus_tag="NBI57_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HCD1823363.1"
                     /translation="MIKPKYKWKLTKPAEYISDELTSKLKLTPIVKKILESKSIIDEQ
                     AIESIISDTDVNHDALQLSDMTKAIERIKRAIANDEKILVYGDYDADGVTSTTILVTT
                     LQLLGAQVGWHIPNRFTEGYGPNELAFRNAHDEGITLIITVDNGIQGHNEIKMVQDLG
                     VDVIVTDHHEIGSTLPEAYAIVHPMHPSFNYPFQQLCGAGVAYKLAQALIENVPDYFK
                     ALVAIGTIADLVSLTDENRSLVKQGLKVLNDQCPTSVKALLKEAGYNDNIDEETIGFI
                     IGPRLNAVGRLDDASLACELLMTDDEEEAAFLAEQVEHFNRERKDIVATITEEAMAMA
                     ETKVKKGDLFLLLAKENWHEGVLGIVASKIVETFALPTLILNIDREQNHAKGSARSID
                     QVSMFEILSAHQELIAKFGGHHMAAGMTMDIENIESLAEGLNKWMKELSKTTSLDPVK
                     PVDVLLTENDITIKNIRDMNRLRPFGTDFSRPIFEMDDLSVSSVKAIGQQKNHLKLTL
                     GESNIAALFWQNGHLEPELQDEQPINILGSVQINEWNGNQSPQLIIQDIAMNEQQILD
                     YRSKRKSLPFTENDENIVVLIHPKSDKVNANEYYYGEEIKQQTDKVVLRDLPTSMEDL
                     SNSLQQLQFSQLYIVLQHNHSIYFDGIPNMDIFKKCYKALITKQETNIQKEGMLLCQH
                     LSVKPDTLKFMLKVFLDLKFVTQEDGLIRINQQPDKRSIDSSKVYQLRQQRMDVEKQL
                     LYQDFSEIKNWIKSQLS"
     gene            2301..2819
                     /locus_tag="NBI57_002442"
     CDS             2301..2819
                     /locus_tag="NBI57_002442"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009559512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HCD1823364.1"
                     /translation="MDLKQYVSEVQDWPKPGVSFKDITTIMDNGEAYGYATDKIVEYA
                     KDRDVDIVVGPEARGFIIGCPVAYSMGIGFAPVRKEGKLPREVIRYEYDLEYGTNVLT
                     MHKDAIKPGQRVLITDDLLATGGTIEAAIKLVEKLGGIVVGIAFIIELKYLNGIEKIK
                     DYDVMSLISYDE"
BASE COUNT         1105 a          400 c          549 g          885 t
ORIGIN      
        1 tatatatgat taaaccgaag tataagtgga aattaactaa accagctgaa tacataagtg
       61 atgaattaac atcaaaatta aagttaacgc caatcgtaaa aaaaatatta gaaagtaaat
      121 cgattattga tgaacaagca attgaatcca tcatttcaga cacagatgtc aatcatgatg
      181 cattgcaatt gagcgatatg actaaggcta ttgaaagaat taaaagagcg atagcgaatg
      241 atgagaaaat cctagtttat ggtgattacg atgccgacgg tgttacctca actacaattt
      301 tagtgacaac attacaactt ttaggtgcac aagtaggatg gcatattcct aaccgcttta
      361 ccgaaggata tggaccaaac gaactagcat ttagaaatgc tcatgatgaa ggcataacat
      421 taatcataac tgttgataat ggcatacaag gtcataatga aattaaaatg gttcaagatt
      481 taggagttga tgtcattgta actgatcatc atgaaattgg tagtacatta ccagaagcat
      541 atgcaatcgt acatccaatg catccatcgt ttaattatcc gttccaacaa ttgtgtggtg
      601 caggtgttgc atacaaatta gcacaagcat tgattgaaaa tgtacctgat tattttaaag
      661 cattagttgc gataggtacc attgcggatt tagtatcctt aactgatgaa aatcgatcat
      721 tagtgaaaca aggattaaaa gtcttaaatg atcaatgtcc gacctctgtt aaagcattgc
      781 taaaagaagc gggatacaat gacaatattg atgaagaaac aattggtttt attattggac
      841 ctagacttaa tgctgtaggt cgtttagatg acgcatcatt agcttgcgaa ttgttgatga
      901 ctgacgacga agaagaagct gcatttttag cagaacaagt tgaacatttt aaccgtgaaa
      961 gaaaagatat tgtagcaact atcacagaag aagcaatggc tatggcagaa acgaaagtta
     1021 aaaagggaga tttattttta cttttagcaa aagaaaattg gcatgaaggt gtcttaggta
     1081 ttgttgcttc gaaaattgtc gaaacatttg ctttgccaac attaatttta aatattgata
     1141 gggagcaaaa tcatgcaaaa ggttccgcca gatcaattga tcaagtgtcg atgtttgaaa
     1201 tactatcagc acatcaagag ttaatcgcta aatttggcgg ccatcatatg gctgcaggta
     1261 tgacgatgga tattgaaaat atagaatcgt tagctgaagg tttaaataaa tggatgaaag
     1321 aattatctaa aacaacttcg ttagatcccg taaagccagt agacgtatta ttaacagaaa
     1381 atgatataac gattaaaaat attcgtgata tgaatcgttt aagacccttc ggaacagatt
     1441 tttctagacc tatttttgaa atggatgatt tatcagtttc ttcagttaag gcgatcggtc
     1501 aacaaaaaaa tcatctaaaa ttgacacttg gtgaaagtaa tatcgccgcg ctattttggc
     1561 aaaatggaca cttagaacct gagttacaag atgaacagcc aattaatata ttaggatctg
     1621 ttcaaatcaa cgaatggaat ggtaatcaat caccgcaatt aattattcaa gatattgcga
     1681 tgaatgaaca gcaaatatta gattatagaa gtaagcgaaa aagtttacct tttacagaaa
     1741 atgatgaaaa tattgtcgtg cttattcatc ctaaaagtga taaagtaaat gcgaatgaat
     1801 attattatgg tgaagaaatt aaacaacaaa ctgataaagt agtattaaga gatttaccaa
     1861 cgtcaatgga agacttgtct aattccttgc aacaactgca attttctcaa ctttatatag
     1921 ttttgcaaca taatcattcg atttacttcg atggtatacc taatatggat atttttaaaa
     1981 agtgttataa agcattaata actaaacaag aaacaaatat ccagaaagag ggtatgttat
     2041 tgtgtcaaca tttaagtgtg aaaccagata cacttaaatt catgttgaaa gttttcttag
     2101 acttaaaatt tgtaacacaa gaagatggtt taattcgaat caatcaacaa cctgataaaa
     2161 gatcgattga ttccagcaaa gtatatcaat taagacaaca acgtatggat gttgaaaagc
     2221 aattattata tcaagatttt tcagaaataa aaaattggat aaagtcacaa ttgtcgtgag
     2281 caatttagga ggaaatatta atggatttaa agcaatacgt atcagaagtt caagattggc
     2341 cgaaaccagg tgttagtttc aaggatatta ctacaattat ggataatggt gaagcatatg
     2401 gctatgcaac agataaaatt gtagaatacg caaaagacag agatgttgat atcgttgtag
     2461 gacctgaagc gcgtggcttt atcattggct gtcctgtagc ttattcaatg gggattggct
     2521 ttgcacctgt tagaaaagaa gggaaattac ctcgtgaagt cattcgttat gagtatgacc
     2581 tagaatatgg tacaaatgtt ttaacaatgc acaaagatgc aattaaacca ggtcaacgtg
     2641 tgttaattac agatgattta ttagctactg gtggtacgat tgaagcagca ataaaattag
     2701 ttgaaaaatt aggcggtatc gtagtaggta ttgcatttat aattgaattg aaatatttaa
     2761 atggtattga aaaaattaaa gattacgatg ttatgagttt aatctcatac gacgaataat
     2821 aaataatata attttatcaa atgaaatcct tcatcaaatg tataagaacc aatgacttaa
     2881 ttaaaaaagt tgtttaagtt ttcttaacat gagatgttag gattttttat ttactgaaa
//
LOCUS       DAHHSC010000240         2917 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.244, whole genome shotgun sequence.
ACCESSION   DAHHSC010000240 DAHHSC010000000
VERSION     DAHHSC010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2917)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2917)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2917
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.244"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            30..884
                     /locus_tag="NBI57_002443"
     CDS             30..884
                     /locus_tag="NBI57_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosaminidase"
                     /protein_id="HCD1823365.1"
                     /translation="MNKHKKGSIFGIIGLVVIFAVVSFLFFSMISDQIFFKHVKSDIK
                     IEKLNVTLNDAAKKQINNYTSQQVSNKKNDAWRDASATEIKSAMDSGTFIDNEKQKYQ
                     FLDLSKYQGIDKNRIKRMLVDRPTLLKHTDDFLKAAKDKHVNEVYLISHALLETGAVK
                     SELANGVEIDGKKYYNFYGVGALDKDPIKTGAEYAKKHGWDTPEKAISGGADFIHKHF
                     LSSTDQNTLYSMRWNPKNPGEHQYATDIKWAESNATIIADFYKNMKTEGKYFKYFVYK
                     DDSKHLNK"
     gene            complement(971..2263)
                     /gene="arsB"
                     /locus_tag="NBI57_002444"
     CDS             complement(971..2263)
                     /gene="arsB"
                     /locus_tag="NBI57_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite efflux transporter membrane subunit
                     ArsB"
                     /protein_id="HCD1823366.1"
                     /translation="MMTTLATLIFLVTLLFVLWQPKGLDIGITALTGAFIAVITGVVS
                     FSDVFEVTGIVWNATLTFVSVILISLILDKVGLFEWSAIHMLHASKGNGLKMFVYIIL
                     LGAIVAAFFANDGAALILTPIVLAMVKNIGFSKRAIFPFIIASGFIADTTSLPLIVSN
                     LVNIISADYFHVGFVRYFSRMIIPNLFSLLASIIVLWLYFRKAIPKTFDDNNIKHPKD
                     AINDLKLFKISWIVLVILLFGYLISEFTKIPVSIFTGIIAFIFLMLARKSNAVNIKQV
                     IKGAPWNIVLFSIGMYIVVFGLRNAGITLILAKILEYISNYGLFSTILGMGFISAFLS
                     SIMNNMPTVLIDAIAIGQSNVHGMLKEGLIYANVIGSDLGPKITPIGSLATLLWLHVL
                     TQKDVKISWGTYFKTGIIITIPVLFITLIGLYLTLIIF"
     gene            complement(2263..2577)
                     /locus_tag="NBI57_002445"
     CDS             complement(2263..2577)
                     /locus_tag="NBI57_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HCD1823367.1"
                     /translation="MTYKELATFLKVLSDSSRLEILDLLSCGELCACDLLAHFQFSQP
                     TLSYHLKALVKTNLVTTRKIGNKHLYQLNHNIFESVINNLSKVHTSNQRCFCHNLKTG
                     EC"
BASE COUNT         1107 a          431 c          471 g          908 t
ORIGIN      
        1 ataacaacaa tgacctaaga ggtgtggata tgaataaaca caagaaaggt tctatttttg
       61 gaataatagg acttgttgtc atatttgctg ttgtctcatt tttatttttc tcaatgatat
      121 ccgatcagat atttttcaaa catgttaaat ccgacattaa gattgaaaag ttaaatgtta
      181 cattaaacga tgcagcaaag aaacaaataa ataattatac gagtcaacag gtatcaaata
      241 aaaagaatga tgcatggaga gatgcatctg caactgaaat taaaagtgca atggatagcg
      301 gtacttttat cgataatgaa aagcaaaaat atcaattttt agatttatca aagtatcaag
      361 ggattgataa aaatagaatt aaacgtatgt tagtagatag accaacgtta ttgaaacata
      421 cggatgattt cttaaaagct gctaaagata agcacgttaa cgaagtttat ttaatttcac
      481 atgcattatt agaaactggc gcagttaaaa gtgaattagc taatggagtc gaaattgatg
      541 gcaaaaaata ctacaatttc tatggagtag gagcccttga taaagaccca attaaaacag
      601 gtgcagaata tgctaaaaag catggttggg atacacctga aaaagctatt tcaggcggtg
      661 ctgatttcat tcataagcac ttcttatcaa gcacagatca aaatacattg tatagtatga
      721 gatggaatcc aaaaaatcca ggagaacatc aatatgctac tgatattaag tgggcagaaa
      781 gtaatgcaac aattatcgct gacttttata agaatatgaa gactgaagga aaatacttca
      841 aatactttgt gtataaagat gacagtaaac atttgaataa gtaatttgat agagttaagt
      901 tatgatataa cttgctcaag tataaaaaga cgctttctat cgcaataaat agaaagcgtc
      961 tttttatttc ttaaaatatg ataagtgtta gatacaaccc tatgagggtt ataaatagta
     1021 ctggaattgt aatgatgata ccagttttaa agtatgtgcc ccaagaaatc ttaacatctt
     1081 tttgtgttaa gacgtgtaac cacagtaatg tagctaaaga gcctatcggt gtaatttttg
     1141 gacctaaatc agaaccgata acattcgcat aaattaggcc ttcttttaac atgccatgga
     1201 catttgattg accaatagca atcgcatcta ttaaaactgt aggcatatta ttcattattg
     1261 atgataaaaa cgctgaaatg aagcccattc ccaaaatggt gctaaataga ccgtaattgg
     1321 aaatatattc taatatttta gccaatatta aagtaatgcc agcatttctt aagccgaata
     1381 cgacgatata cataccaatt gaaaataata ctatattcca aggtgcgccc ttaatgactt
     1441 gcttaatatt tacagcattt gatttacgag ccaacattag aaaaataaaa gcaatgattc
     1501 cagtgaaaat tgataccgga attttagtaa attcactgat tagatagccg aaaagtaata
     1561 taactagaac aatccatgaa attttaaata gctttaaatc attaatggca tctttaggat
     1621 gctttatatt attatcatca aacgttttag gtatcgcctt tctaaaatat aaccacaata
     1681 ctataatact tgctaaaagc gagaataaat taggtataat cattctacta aaatatcgaa
     1741 cgaatcctac atgaaaataa tcagcagata taatattcac tagattgctc acgatcaaag
     1801 gtaaagaagt tgtgtcagct ataaaaccac tcgcaataat aaaagggaat atggcccgct
     1861 tactaaaacc tatattttta accatcgcta atacaatagg cgttaagatt aacgctgcgc
     1921 catcatttgc gaaaaatgca gcaacaatgg cacccaataa tatgatataa acgaacattt
     1981 ttaaaccatt tccttttgaa gcatgaagca tgtgaatagc tgaccattcg aataatccaa
     2041 ctttatctaa tattaatgaa ataagaatga ctgagacaaa agtcaaagta gcattccaaa
     2101 caatacctgt tacttcgaaa acatcggaaa aacttacaac accagtaata acagcaataa
     2161 aggcaccagt taatgcggta atgccaatat ctaggccttt aggttgccat aatacaaata
     2221 ataaagttac tagaaaaatg agtgtcgcta aagttgtcat cattagcatt caccagtctt
     2281 aaggttatga caaaaacatc gttggttaga ggtatgaacc ttagacaagt tattaattac
     2341 ggactcaaaa atattatgat taagctggta taaatgttta tttccgattt ttcgtgtcgt
     2401 aactaagttg gtttttacta atgctttcaa atgatagcta agtgtgggtt gagagaattg
     2461 aaaatgtgct aacaaatcac aagcgcataa ctctccacaa gaaagtaaat ctagtatttc
     2521 taatctgctt gaatctgata aaacttttaa aaatgttgct agttctttat acgtcataac
     2581 atacctccta gacgttaaat agattatcat ctatatagat gaatgtctat gttcctttgg
     2641 tatattacac gatatgacta tgtaatttga atttggtttt agtattaaaa gggtattaaa
     2701 gataaattat agatattgat tttgcaaaat atactctttg ttttgcattg aaaaaggtga
     2761 ttagccatat cttgagtgta tggccaatca ccttagttga atttattttg taatcaaggg
     2821 tattgattaa gaagctttgt ttttacgact aaaaatcata aatagaatta ctaatgagat
     2881 taatatcttt gctataaatt tcaatttaat tcacctc
//
LOCUS       DAHHSC010000241         2906 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.245, whole genome shotgun sequence.
ACCESSION   DAHHSC010000241 DAHHSC010000000
VERSION     DAHHSC010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2906)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2906)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2906
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.245"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            complement(179..2788)
                     /gene="adhE"
                     /locus_tag="NBI57_002446"
                     /gene_synonym="adhC"
     CDS             complement(179..2788)
                     /gene="adhE"
                     /locus_tag="NBI57_002446"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HCD1823368.1"
                     /translation="MLTIPEKENRGSKEQEVAIMIDALADKGKKALEALSKKSQEEID
                     HIVHQMSLAAVDQHMVLAKLAHEETGRGIYEDKAIKNLYASEYIWNSIKDNKTVGIIG
                     EDKEKGLTYVAEPIGVICGVTPTTNPTSTTIFKAMIAIKTGNPIIFAFHPSAQESSKR
                     AAEVVLEAAMKAGAPKDIIQWIEVPSIEATKQLMNHKGIALVLATGGSGMVKSAYSTG
                     KPALGVGPGNVPSYIEKTAHIKRAVNDIIGSKTFDNGMICASEQVVVIDKEIYKDVTN
                     EFKAHQAYFVKKDELQRLENAIMNEQKTGIKPDIVGKSAVEIAELAGIPVPENTKLII
                     AEISGVGSDYPLSREKLSPVLALVKAQSTKQAFQICEDTLHFGGLGHTAVIHTEDETL
                     QKDFGLRMKACRVLVNTPSAVGGIGDMYNELIPSLTLGCGSYGRNSISHNVSATDLLN
                     IKTIAKRRNNTQIFKVPAQIYFEENAIMSLTTMDKIEKVMIVCDPGMVEFGYTKTVEN
                     VLRQRTEQPQIKIFSEVEPNPSTNTVYKGLEMMVDFQPDTIIALGGGSAMDAAKAMWM
                     FFEHPETSFFGAKQKFLDIGKRTYKIGMPENATFICIPTTSGTGSEVTPFAVITDSET
                     NVKYPLADFALTPDVAIIDPQFVMSVPKSVTADTGMDVLTHAMESYVSVMASDYTRGL
                     SLQAIKLTFEYLKSSVEKGDKVSREKMHNASTLAGMAFANAFLGIAHSIAHKIGGEYG
                     IPHGRANAILLPHIIRYNAKDPQKHALFPKYEFFRADTDYADIAKFLGLKGNTTEALV
                     ESLAKAVYELGQSVGIEMNLKSQGVSEEELNESIDRMAELAFEDQCTTANPKEALISE
                     IKDIIQTSYDYKQ"
BASE COUNT          825 a          556 c          457 g         1068 t
ORIGIN      
        1 aaatgagtat caatcttaat attttcttta tttaaaaaaa tgattgtcta gtttgtatct
       61 ctgaagattt ggcaataaat aaaagccgat aaccgtaaaa tgattatcga cttaaagttt
      121 atgtggcatt ttttactttt gtaatttcag gtgagttata tgattattat cagatagatt
      181 attgcttata atcatatgat gtttgaatga tatctttgat ttcactgatt agtgcttctt
      241 taggattagc agttgtacat tgatcttcaa atgcgagctc tgccattcta tcaattgatt
      301 catttaattc ttcttcagac acaccttgtg atttcaaatt catttcaatt ccgactgatt
      361 gacctaattc gtagacagct ttagctaatg attctacgag tgcttctgtc gtattccctt
      421 ttaatcctaa gaatttggca atatctgcat aatctgtatc tgctctgaag aactcatatt
      481 tagggaataa tgcatgtttt tgcgggtctt tggcattata acggataata tgcggtagta
      541 atatcgcatt cgctctacca tgcggaatac catattcgcc accaatttta tgtgcaattg
      601 agtgtgcaat gcctaagaat gcatttgcaa atgccatacc agccaaagtt gatgcgttat
      661 gcattttctc tcttgaaact ttatcaccct tttcaacaga tgattttaaa tattcgaacg
      721 tcaatttaat cgcttgtaga ctcaaacctc ttgtatagtc tgaagccatt acagatacat
      781 atgattccat tgcatgcgtt agtacatcca ttcctgtatc tgctgtaacg ctttttggca
      841 cactcatcac aaattgaggg tcaataattg caacgtcagg tgttaaagca aaatcagcca
      901 acggatattt tacatttgtt tcactatctg tgataactgc aaatggtgtt acttctgaac
      961 ctgtacctga tgtcgtaggg atacaaatga acgtcgcatt ttcaggcatg cctattttat
     1021 aagtacgttt accgatgtct aggaactttt gtttagcacc gaagaatgat gtctcagggt
     1081 gttcaaagaa catccacatt gcttttgcag catccatcgc tgaaccacca ccaagtgcaa
     1141 tgattgtatc tggttggaaa tcaaccatca tttccagacc tttatatact gtattagttg
     1201 atgggttcgg ttcgacttcg ctaaatattt taatttgagg ctgttccgtt ctttgtctta
     1261 atacattctc aactgttttt gtataaccga attctaccat accagggtca cagacaatca
     1321 tcactttttc aatcttgtcc attgttgtta gactcatgat tgcattttct tcaaaataaa
     1381 tttgagcagg caccttgaaa atttgagtat tattacgtcg tttagcaatc gttttaatgt
     1441 ttaataaatc tgtcgcacta acattatgtg aaattgagtt tctaccgtac gaaccacaac
     1501 ctaatgttaa agacggaatc aattcgttat acatatcacc aatacctcca accgctgatg
     1561 gtgtatttac aagtacacga caagctttca ttcttagtcc aaaatctttt tgtaatgttt
     1621 catcttctgt atggataacg gctgtgtgtc ctaatccacc aaaatgtagt gtgtcttcac
     1681 aaatttgaaa tgcttgtttt gtagattggg cttttactaa ggctaatact ggagataatt
     1741 tttcacgaga taacggatag tctgaaccta caccgctaat ttcggctatg ataagttttg
     1801 tattttcggg gacaggtata cctgctaatt cagctatttc aactgcagat ttaccgacaa
     1861 tatcaggctt aatacctgtt ttttgttcat tcataattgc attttctaag cgttgtaatt
     1921 catctttttt aacaaagtat gcttgatgtg ctttaaattc attagtaaca tctttataaa
     1981 tttctttatc aatgactaca acttgttcag aagcacaaat cataccatta tcaaatgttt
     2041 ttgaaccaat gatatcattt actgcacgtt taatgtgtgc tgttttttca atgtaagacg
     2101 gcacgttacc tggtcccaca cctaatgccg gtttgccagt tgaatatgca gacttaacca
     2161 tgcccgaacc acctgttgct agaactaatg caataccttt gtgattcatt aattgttttg
     2221 ttgcttcgat agaaggcact tcaatccact gaataatatc tttaggtgca cctgccttca
     2281 ttgccgcttc taatacaact tctgctgcac gcttcgacga ttcttgtgca cttggatgga
     2341 atgcaaaaat gattggattt cctgtcttaa ttgcaatcat cgctttaaaa atagttgtcg
     2401 acgtaggatt tgttgttggc gtaacaccac aaataacacc aattggttcc gctacatacg
     2461 ttaatccttt ttctttatct tcaccaataa tccctactgt cttattgtct tttattgaat
     2521 tccatatata ttcagaagcg tataaatttt taatcgcttt atcttcgtat atacctcttc
     2581 cagtttcttc atgtgctaat tttgctagca ccatatgttg atcaacagct gctaagctca
     2641 tttgatgaac aatatgatca atttcttctt gtgacttttt agataatgct tctaatgctt
     2701 ttttcccttt gtcagctaga gcatcaatca taattgccac ttcttgttct ttcgatccac
     2761 gattttcttt ttcaggtata gttaacatat acaaccactc ctttatactt tgtgaattat
     2821 ttcacaaaca ttatagtaca tgtctctcag gatataaaga aaattctata caaaaaagtt
     2881 taatttcgaa tattatttga acaaat
//
LOCUS       DAHHSC010000242         2894 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.246, whole genome shotgun sequence.
ACCESSION   DAHHSC010000242 DAHHSC010000000
VERSION     DAHHSC010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2894)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2894)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2894
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.246"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            complement(11..709)
                     /locus_tag="NBI57_002447"
     CDS             complement(11..709)
                     /locus_tag="NBI57_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron transporter"
                     /protein_id="HCD1823369.1"
                     /translation="MSVGILIFVISVIISIITTMRENSHKDRQNQKPPQKTSTDNEPK
                     KGGFFEEIERTFKEISEELNEEEKKSSKRKYDDTLPPLFDELPKEEPKSKPVVEPMAP
                     KKQPETKPMTEKPITVPKAEPVEQKHRPSRQDNSDEIRRQLEKSLRDDIKTIRTDIDR
                     EKEKQIAKMEKRARDIIEDKYLSERTKRLKLKQLLNSQNVEKDLTKSAFQFDKDEVIN
                     GMIWSEILAKPKQL"
     gene            complement(726..1715)
                     /gene="floA"
                     /locus_tag="NBI57_002448"
     CDS             complement(726..1715)
                     /gene="floA"
                     /locus_tag="NBI57_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500186.1"
                     /note="flotillin-like protein involved in membrane lipid
                     rafts; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin-like protein FloA"
                     /protein_id="HCD1823370.1"
                     /translation="MFSLSFIVIAVIIIVALLILFSFVPIGLWISALAAGVHVGIGTL
                     VGMRLRRVSPRKVIAPLIKAHKAGLALTTNQLESHYLAGGNVDRVVDANIAAQRADID
                     LPFERAAAIDLAGRDVLEAVQMSVNPKVIETPFIAGVAMNGIEVKAKARITVRANIAR
                     LVGGAGEETIIARVGEGIVSTIGSSKHHTEVLENPDNISKTVLSKGLDSGTAFEILSI
                     DIADVDISKNIGADLQTEQALADKNIAQAKAEERRAMAVATEQEMKARVQEMHAKVVE
                     AESEVPLAMAEALRSGNISVKDYYNLKNIEADTGMRNAINKRTDQSDDESPEH"
     gene            complement(1733..2416)
                     /locus_tag="NBI57_002449"
     CDS             complement(1733..2416)
                     /locus_tag="NBI57_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HCD1823371.1"
                     /translation="MTTILESTAGDTWVEQVSNIIVQPIFTLILTCLTFLGFVYQLYS
                     KKINAAGIIATLSLLILFLGFLIQGNVNMHSILIFSIGVILVVIELFVVGAVIGIIGM
                     ILITISITTLGDNLLFMLANVIVALILTIVEWVILVKIFNRKIPFLDKVILKDSTNSE
                     SGYNSHDNRSHLVGKTAQTVTDLRPAGIIFCENERIDAVSDGNFILRNKTVKILEVEG
                     TRVVVREVD"
     gene            complement(2660..2836)
                     /locus_tag="NBI57_002450"
     CDS             complement(2660..2836)
                     /locus_tag="NBI57_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016998564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HCD1823372.1"
                     /translation="MSKTVVRKNESLEDALRRFKRSVSKSGTIQEVRKREFYEKPSVK
                     RKKKSEAARKRKFK"
BASE COUNT          880 a          552 c          417 g         1045 t
ORIGIN      
        1 tcaaaaaatt ttataattgt tttggtttag ctaaaatttc tgaccatatc ataccattga
       61 ttacttcatc tttatcaaat tggaacgctg atttagtcaa atctttttcg acattttgag
      121 aattaagcaa ctgctttaat ttcaaacgtt ttgtacgttc agataagtat ttatcctcaa
      181 taatatctct agcacgtttt tccattttag caatttgctt ttctttttct ctatcaatgt
      241 cagtacgaat cgttttaata tcatctctaa gtgatttttc taattgacgt ctaatttcgt
      301 cagaattatc ttgtctagaa ggtctatgtt tctgctccac cggttctgct ttaggcactg
      361 taattggttt ctctgtcatc ggttttgttt ctggttgttt tttaggtgcc ataggttcta
      421 caacaggttt cgatttaggc tcttcctttg gaagttcatc gaataaaggt ggtaacgtat
      481 catcatattt tcgtttcgat gatttctttt cttcttcatt taattcttca cttatttctt
      541 taaacgttcg ctcaatttct tcaaaaaagc cacctttttt tggttcatta tcggtagatg
      601 ttttttgagg tggcttttga ttttgtctat ctttatgact attttcgcgc atagtagtaa
      661 tgatagaaat gatcactgat atgacaaaaa ttagaatacc gacactcatt taatcacctc
      721 tcgacttaat gttcaggtga ctcatcatca ctttgatcag ttcgtttatt aattgcattt
      781 ctcatgcctg tatcagcttc gatatttttc aaattataat aatctttaac actgatatta
      841 cctgaacgta atgcttcagc catagctaat ggtacttcag attcggcttc aactacttta
      901 gcatgcattt cttgtacacg cgctttcatt tcttgctcag ttgctacagc catagctcta
      961 cgttcttcag cttttgcttg tgcaatattt ttgtctgcta atgcttgttc agtttgtaag
     1021 tctgcaccaa tatttttact aatatcaacg tcagcaatat caattgataa aatttcaaat
     1081 gcagtacctg aatctaaacc tttgcttaaa actgttttag aaatattatc tgggttttca
     1141 agtacttctg tatgatgctt actagaacca attgttgaaa cgataccttc accaacacgt
     1201 gcgatgattg tttcttcacc tgcaccacca acaagtcgag caatattagc tctaactgtg
     1261 atacgagctt tggctttcac ttcaataccg ttcattgcta cacctgcgat aaatggtgtt
     1321 tcaatgactt taggattaac agacatttga accgcttcta atacgtcacg tcctgcaagg
     1381 tcaattgcag cagcacgttc gaaaggaaga tcaatgtcag cacgttgtgc agcaatatta
     1441 gcatcaacaa ctctgtcaac atttcctcct gctagataat gcgattctaa ttggtttgtt
     1501 gttaatgcta gtcctgcttt gtgcgcttta attaatggcg ctataacttt tcttggagat
     1561 acacgacgta aacgcatacc aaccaatgta cctataccaa catgaacgcc agctgctaac
     1621 gctgaaatcc acaaaccaat cggtacaaat gagaataaaa taagtaatgc aactattata
     1681 ataactgcta ttacgataaa acttaaacta aacatggtat cgctcctttt aattaatcta
     1741 cttccctcac aactactctt gttccttcaa cttcaaggat tttcaccgtt ttattgcgta
     1801 aaataaagtt gccatctgaa acagcatcaa tacgttcatt ttcacaaaaa ataatccctg
     1861 caggtcgaag atctgtaact gtttgagcag tctttcctac gaggtgcgag cggttatcat
     1921 gagaattgta acctgactca gaattagttg aatcttttaa gataacctta tccaaaaacg
     1981 gaatctttct gttgaaaatc ttcactaata tcacccattc tacaatcgtt aaaatcaagg
     2041 caacgataac attcgcaagc ataaatagca aattatcacc gagcgttgtt atgcttatag
     2101 ttatcagtat catgccaata ataccaatta ctgcaccaac tacaaataat tcaattacaa
     2161 ctaatataac gccaattgag aatattaaga tagaatgcat attgacattt ccttggatta
     2221 gaaatcccaa aaataaaata agtaatgata atgtggcgat aataccagct gcattgattt
     2281 ttttagagta aagttgatat acaaatccta agaatgtcaa acaggttaat attaacgtaa
     2341 aaataggttg aacaattata ttactaactt gttcaaccca agtatctcca gccgttgatt
     2401 ccaagatagt tgtcatttgt aaaaaattat tataactcac aatttcacct cgccctcaca
     2461 ttaattatac atgaaattga atatacttta tagttataag aacctataga atatcaattt
     2521 gattatttat aaactatata acttttttta attaaaaatt gacaacaaaa aaacatcaca
     2581 tattagtgtc agacactaac ttgtgatgtt tattttatat aattaatatt cgtgttgagg
     2641 gagtcaacag aggtattaat tatttgaatt tacgtttacg tgcagcttct gatttctttt
     2701 tacgttttac gcttggtttt tcgtaaaatt cacgtttacg tacttcttgg attgttccac
     2761 ttttagaaac tgaacgttta aatctacgta acgcatcttc aagtgattca tttttacgta
     2821 ctactgtttt agacatctgt atttccctcc ctccaaatat caacggaact ttatagtcaa
     2881 ttgcacagta aaac
//
LOCUS       DAHHSC010000243         2893 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.247, whole genome shotgun sequence.
ACCESSION   DAHHSC010000243 DAHHSC010000000
VERSION     DAHHSC010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2893)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2893)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2893
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.247"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            complement(<1..430)
                     /gene="deoC"
                     /locus_tag="NBI57_002451"
     CDS             complement(<1..430)
                     /gene="deoC"
                     /locus_tag="NBI57_002451"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HCD1823373.1"
                     /translation="MNSAKLIDHTLLKPESTRTQIDQIIDEAKAYHFKSVCVNPTHVK
                     YAAERLADSEVLVCTVIGFPLGASTTATKAFETEDAIQNGADEIDMVINIGALKDGRF
                     DDVQQDIEAVVKAAKGHTVKVIIETVLLDHDEIVKASELTK"
     gene            744..1454
                     /gene="deoD"
                     /locus_tag="NBI57_002452"
     CDS             744..1454
                     /gene="deoD"
                     /locus_tag="NBI57_002452"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HCD1823374.1"
                     /translation="MTKGTPHIQPNGVKIAKTVLMPGDPLRAKYIADNFLENVEQFND
                     VRNMFGYTGTYKGKEVSVMGSGMGIPSIGIYSYELYNFFDVDTIIRIGSCGALQENVN
                     LYDVIIAQAASTNSNYVDQYNIPGHFAPIADFELVTKAKNVADQIGATTHVGNVLSSD
                     TFYNADPTFNDAWKKMGILGIEMESAGLYLNAIHAGKKALGIFTVSDHILRDEATTPE
                     ERQNSFTQMMEIALEIAE"
     gene            complement(1575..2018)
                     /locus_tag="NBI57_002453"
     CDS             complement(1575..2018)
                     /locus_tag="NBI57_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HCD1823375.1"
                     /translation="MSNQQDVVKELNQQVANWTVAYTKLHNFHWYVKGPNFFSLHVKF
                     EELYNEASQYVDELAERILAVGGNPVGTLTECLEQSIVKEAAKGYSAEQMVEELSQDF
                     TNISKQLENAIEIAGNAGDDVSEDMFIGMQTSVDKHNWMFKSYLS"
     gene            complement(2218..2631)
                     /locus_tag="NBI57_002454"
     CDS             complement(2218..2631)
                     /locus_tag="NBI57_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol-disulfide oxidoreductase DCC family
                     protein"
                     /protein_id="HCD1823376.1"
                     /translation="MPIVYYDGNCIYCYNYVIWLIQHDLPRNYQFATLKGEVGQQFFK
                     QHPEAANKNSVILQKGDRIYFESQAIIKLITALPNRTKLLGVGLWLVPKPVRNFGYRM
                     FANNRNRMWKTTWHQPNDYEKSFFLDDNAKVKLTD"
BASE COUNT          890 a          503 c          424 g         1076 t
ORIGIN      
        1 cttttgttaa ttcactcgct tttacgattt cgtcatggtc caacaatacc gtctcaataa
       61 tcacttttac tgtgtgacct ttcgcagctt tcaccactgc ttcaatgtct tgttgtacat
      121 catcaaaacg tccatctttt aatgcgccga tgttgatgac catgtcaatt tcatctgcac
      181 cattttgaat cgcatcttct gtttcaaatg ctttcgtcgc agttgtcgat gcacctaatg
      241 ggaatcctat taccgtacaa actaacacct ctgaatcagc tagtcgctct gctgcatatt
      301 taacatgcgt tggattcaca catacagatt taaaatggta tgctttcgct tcatcgatga
      361 tttgatcgat ttgcgtacgt gttgactcag gcttcaataa agtgtgatca atcaattttg
      421 cactattcat ttctatctcc tcctttatgg ttgattataa aaatacggtt gtaaattagt
      481 tgattgagcg tcaggttcat ttaaatatca ggttagatgt tcgcttttta tgtaaccgca
      541 tacatatact attacattaa ttcatttccc ataaacaaac aatacaattg aacgtgatat
      601 cttcattatg aacgatgact tgacaacaag ctaatcagga ttatattttt ataattcttt
      661 aattctatag tacaaaaatt cgcaaaaaag ggaaacaaat gttatcttaa aattattaat
      721 gaatattaag gagaagataa caaatgacaa aaggtacacc acatattcaa ccaaatggag
      781 taaaaattgc taaaacagta ttaatgcctg gcgatccgct acgcgcaaaa tatattgctg
      841 ataatttttt agaaaatgtt gaacaattta acgatgtacg taacatgttt ggttacactg
      901 gtacatataa aggtaaagaa gtttctgtaa tgggttctgg tatgggtatt ccaagtattg
      961 gtatttactc atatgagtta tacaacttct ttgatgtaga tacaatcatt cgtatcggtt
     1021 cttgtggcgc attacaagaa aatgttaact tatacgatgt tattattgca caagctgcat
     1081 caactaattc aaattatgta gatcaataca atattccagg tcatttcgcg cctatcgctg
     1141 acttcgagtt agtaactaaa gctaaaaatg tcgctgacca aatcggtgct actacacacg
     1201 taggtaacgt attatcttct gatacatttt acaatgccga tccaacattc aatgatgctt
     1261 ggaaaaaaat gggtatttta ggtatcgaaa tggaatcagc tggtttatat ttaaatgcga
     1321 ttcatgctgg taaaaaagca cttggtattt tcacagtaag tgatcatatt ttacgtgacg
     1381 aagctactac acctgaagaa cgtcaaaatt catttacaca aatgatggaa atcgctttag
     1441 aaatcgcaga gtaacttatt taaattgact ttaattgctc tttaacaatg cgattaaact
     1501 caaaaagcca acacattctg ggcgtatccc catttatgtg ttggctttta tttatattat
     1561 tacttatctg tagattagct taagtaagat ttaaacatcc aattatgttt atctactgat
     1621 gtttgcatac ctataaacat atcttctgat acatcatcgc cagcattacc agcaatttcg
     1681 attgcgtttt ctaattgttt tgagatattt gtgaagtctt gtgataattc ttcaaccatt
     1741 tgttctgcag agtaaccttt cgcagcttct ttaacaattg attgctctaa gcattcagtt
     1801 aatgtaccta cagggtttcc tcctaccgct aaaattcttt cagctaattc gtctacatat
     1861 tggcttgctt cattatataa ttcttcaaat ttaacgtgta atgagaagaa gttaggtcct
     1921 ttcacatacc agtggaaatt atgtagcttt gtgtaagcta ctgtccagtt tgctacttgt
     1981 tgattcaatt cttttacaac atcttgttga ttactcatat ttaatacact ccttaaaatt
     2041 gtctacgtct tgcttgttta tattaattat tatattataa tgattctaat ctgtaaagtg
     2101 tttttactct cttttttata attattctaa tctaaatgta aggtgttccg ctttttaaat
     2161 gtctaaattt taattcatca tctagtatat ccattatcaa ttagcaagta tgttttatca
     2221 atcagtaagt tttactttcg cattatcatc taaaaagaac gatttttcat aatcatttgg
     2281 ttggtgccat gttgttttcc acattctatt tctattatta gcaaacattc gataaccgaa
     2341 attacggact ggtttaggta caagccataa acctacgccc aataatttag ttcgattagg
     2401 tagtgctgtg attagtttaa taatcgcctg cgattcaaaa tatatacgat cacctttctg
     2461 taaaatcaca ctatttttat ttgccgcttc tggatgttgt ttaaaaaatt gttgtccaac
     2521 ttcacctttt aacgtggcaa attgataatt tcttggtagg tcatgttgaa ttaaccaaat
     2581 gacatagtta taacaatata tacagtttcc atcataatat acgattggca taatttattc
     2641 gctcctttaa tagtacttat agtcattttt cctaatttaa aaacatttaa acaaggttgt
     2701 cacttgataa gtcctcgcga cacgaaattg ccataaaatt tatttttcag ttttatattt
     2761 attgtataat gtatttttta tttactttaa atttctcaat ctacagtgta atcttatttc
     2821 ttattattaa cttagcgttt ggtcattcgt cttattcaac atacgactgg aaaactcata
     2881 taaagtttta att
//
LOCUS       DAHHSC010000244         2837 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.248, whole genome shotgun sequence.
ACCESSION   DAHHSC010000244 DAHHSC010000000
VERSION     DAHHSC010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2837)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2837)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2837
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.248"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            complement(22..1311)
                     /locus_tag="NBI57_002455"
     CDS             complement(22..1311)
                     /locus_tag="NBI57_002455"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HCD1823377.1"
                     /translation="MNFSESERLQQLSNEYILGGVNSPSRSYKAVGGGAPVVMKEGHG
                     AYLYDVDGNKFIDYLQAYGPIIAGHAHPHITKAIQEQAAKGVLFGTPTELEIEFSKKL
                     RDAIPSLEKIRFVNSGTEAVMTTIRVARAYTKRNKIIKFAGSYHGHSDLVLVAAGSGP
                     SQLGSPDSAGVPESVAREVITVPFNDINAYKEAIEFWGDEIAAVLVEPIVGNFGMVMP
                     QPGFLEEVNEISHNNGTLVIYDEVITAFRFHYGAAQDLLGVIPDLTAFGKIVGGGLPI
                     GGYGGRQDIMEQVAPLGPAYQAGTMAGNPLSMKAGIALLEVLEQDGVYEKLDSLGQQL
                     EEGLLKLIEKHNITATINRIYGSLTLYFTDEKVTHYDQVEHSDGEAFGKFFKLMLNQG
                     INLAPSKFEAWFLTTEHTEEDIQQTLKAADYAFSQMK"
     gene            1392..1847
                     /gene="bcp"
                     /locus_tag="NBI57_002456"
     CDS             1392..1847
                     /gene="bcp"
                     /locus_tag="NBI57_002456"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HCD1823378.1"
                     /translation="MLQKGEQFPIFKLENQDGTVITNDTLKGKKAIIYFYPRDNTPTC
                     TTEACDFRDNLEMFNDLDVAVYGISGDSKKKHQNFIEKHGLNFDLLVDEDFKLAKETG
                     VYQLKKSFGKESMGIVRTTFIIDEQGKVLDVIEKVKVKTQIEELKNILG"
     gene            1853..2803
                     /locus_tag="NBI57_002457"
     CDS             1853..2803
                     /locus_tag="NBI57_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HCD1823379.1"
                     /translation="MKVVGLNRMREVETELQQRFSDLDFKFYKKASEIPESDLADLDI
                     LVGYDGGINEAFLQRCPNLKWIAWFATGVNTLPLDYIADHGILLTNGKGVQAKQLSEY
                     ILAFILDDYKKMKLSYDNQRQHIYDSKITGKRLSGQTVLFLGTGAIATRTAKLAKAFN
                     MNLIGLSKSGQNKDEFDEIYTIESLESTLPNADIIINALPETQETIHLLKKKHFELMK
                     DEALFINIGRGSIVKEALLIEVLKSKVIRHAYLDVFENEPLKPNHELYELENVTITAH
                     ITGNDYEAKYDLLDIFKNNLVNFLNKNGLIENEVDAKKGY"
BASE COUNT         1005 a          440 c          487 g          905 t
ORIGIN      
        1 aaaaatatac ttccttaaaa gttatttcat ttgactaaaa gcatagtctg cagcttttaa
       61 agtttgttga atatcttctt ctgtatgttc agttgttaag aaccaagctt caaacttaga
      121 aggtgctaaa ttgatacctt gatttaacat taatttgaaa aatttaccga acgcttcgcc
      181 gtcagaatgt tcaacttgat cataatgtgt gactttttca tctgtaaagt acaatgttaa
      241 agatccataa atacgattaa ttgtagctgt gatattatgt ttttcgatta atttaagtaa
      301 accttcttct agttgttggc ctaagctgtc taatttttca taaacaccgt cttgttctag
      361 tacttcgagt aatgcaatac ctgctttcat agataacggg ttaccagcca ttgtaccagc
      421 ttgatatgca ggtcctagag gtgctacttg ttccataata tcttgacgtc caccatagcc
      481 tccaattggt aaaccaccgc caacaatttt accaaatgca gttaaatcag ggataacacc
      541 taataaatct tgagcggcac cgtaatggaa acggaatgca gtaattactt catcataaat
      601 cactagtgtc ccattgttat gcgaaatttc attaacctct tctaaaaatc caggttgagg
      661 cattaccatt ccaaagttac caacaattgg ttctactaat actgcggcaa tttcatcacc
      721 ccaaaattca attgcttctt tatatgcgtt aatatcattg aaaggtacag taatgacttc
      781 acgtgcgacg ctttctggaa caccagctga gtctggagaa ccgagctgag atgggccact
      841 acctgctgca accaatacta aatcagaatg gccatgataa gatccagcaa attttataat
      901 tttatttctt ttagtatatg cacgtgcaac acgaattgtt gtcatgactg cttctgttcc
      961 agaatttaca aagcgaattt tctcaagaga tggaattgca tcacgtaatt ttttgctgaa
     1021 ttcaatttct agttcagtcg gtgtaccaaa taaaacacct ttggctgctt gttcttgaat
     1081 tgctttagta atatgaggat gtgcatgccc cgcaataatt ggaccgtatg cttgaaggta
     1141 atcaataaat ttattgccat cgacatcata taaatatgca ccgtgtcctt ctttcataac
     1201 aacaggtgca ccgcctccta cagctttata agaacgagaa ggggaattga caccgcctag
     1261 aatatattcg tttgaaagtt gttgtaaacg ttcactttca ctaaaattca tttatatcaa
     1321 cctcttttaa tttaatattt tcatctaata tcgtatcata aaattattat aatgaagaaa
     1381 aaggtgatta tatgttgcaa aaaggagaac aatttccaat atttaaatta gaaaatcaag
     1441 acggaactgt cattacaaat gatacattaa aaggtaaaaa ggcgattata tatttttatc
     1501 ctagagataa tacacctact tgtaccacag aagcttgtga ctttagagac aatttagaaa
     1561 tgttcaatga tttagatgtt gcagtatatg gtataagcgg tgattcaaag aaaaaacacc
     1621 aaaattttat tgagaaacac ggattgaatt tcgatttatt agtagatgaa gattttaaat
     1681 tagctaaaga aacaggcgta tatcagttaa aaaaatcatt tggcaaagaa agtatgggta
     1741 ttgtaagaac aacttttata atagatgaac aaggtaaagt attagatgtt atcgagaagg
     1801 ttaaggttaa aacacaaata gaagaactta aaaacatttt ggggtgacat atatgaaagt
     1861 tgttgggtta aatcgtatgc gtgaagttga aactgaatta caacaacgct tttcagattt
     1921 agattttaaa ttttataaaa aagcatcaga aatacctgag agtgacttgg ctgatttaga
     1981 tatattagtt ggttatgatg gcggtatcaa tgaggcattt ttacaacgtt gcccgaattt
     2041 aaaatggatt gcatggtttg caacgggtgt aaatacatta ccgttagatt atattgcaga
     2101 tcacggcata cttttaacta atggaaaagg tgttcaagct aaacaattat ctgaatacat
     2161 tttagctttc attttagatg attataaaaa gatgaaacta tcatatgata accaaagaca
     2221 acatatatat gattcgaaaa taactggtaa acgcctatca ggacaaacag ttttattttt
     2281 aggtacaggt gcaattgcta ctagaactgc gaagttagca aaggctttta atatgaattt
     2341 aattggtctg agcaagtcag gtcagaacaa agatgagttt gacgagatat atactatcga
     2401 atcacttgaa agcacattac caaatgctga cattattata aatgctttac cagaaacgca
     2461 agaaacgatt catttactaa agaaaaaaca ttttgaatta atgaaagatg aagcactttt
     2521 tataaatata ggacgaggta gcatagttaa agaagcgctc ttaatagaag tattaaaaag
     2581 taaagttatt cgacatgcat atttagatgt gtttgaaaat gaacctttga aacctaatca
     2641 tgaattatat gaattggaaa atgtaactat aacagcgcat ataactggta atgattatga
     2701 ggcaaagtat gacttattag atatttttaa aaacaatcta gttaattttc tcaataagaa
     2761 tggtctaatt gagaatgaag ttgatgctaa aaaaggctat taaatgaaat catcatgtaa
     2821 atattgacac gcgcgca
//
LOCUS       DAHHSC010000245         2810 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.249, whole genome shotgun sequence.
ACCESSION   DAHHSC010000245 DAHHSC010000000
VERSION     DAHHSC010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2810)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2810)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2810
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.249"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            93..1226
                     /locus_tag="NBI57_002458"
     CDS             93..1226
                     /locus_tag="NBI57_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HCD1823380.1"
                     /translation="MINEQRLLNTFLELVQIDSETGNESTIQPILKEKFIALGLDVKE
                     DEAAKHPKLGANNLVCTMNSTIEEGEVPKLYLTSHMDTVVPAINVKPIVKDDGYIYSD
                     GTTILGADDKAGLAAMLEVLQVIKEQQIPHGQIQFVITVGEESGLIGAKELNSELLDA
                     DFGYAIDASADVGTTVVGAPTQMLISAKIIGKTAHASTPKEGVSAINIAAKAISRMKL
                     GQVDEITTANIGKFHGGSATNIVADEVILEAEARSHDPERIKTQVKHMTDVFETTASE
                     LGGKAEVTVEQSYPGFKINDNEAVVKIAQESARNLGLSANTIISGGGSDGSIINTFGI
                     PSVILGVGYEKIHTTNERMPIKSLNLLASQVLEIIKIVARQSK"
     gene            1294..2700
                     /gene="gndA"
                     /locus_tag="NBI57_002459"
     CDS             1294..2700
                     /gene="gndA"
                     /locus_tag="NBI57_002459"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HCD1823381.1"
                     /translation="MTQQIGVIGLAVMGKNLAWNIESRGYSVSVFNRSSEKTDLMVEE
                     SKGKNIHPTYSLEEFVNSLEKPRKILLMVQAGKATDATIDSLLPLLDDGDILIDGGNT
                     NYQDTIRRNKALAQSAINFIGMGVSGGEIGALTGPSLMPGGQEEAYNKVADILDAIAA
                     KAKDGASCVTYIGPNGAGHYVKMVHNGIEYADMQLIAESYAMMKELLGMSHEDIAQTF
                     KDWNAGELESYLIEITGDIFMKLDENKEALVEKILDTAGQKGTGKWTSINALELGIPL
                     TIITESVFARFISSIKEERVNASKELNGPKASFDGDKKDFLEKIRKALYMSKICSYAQ
                     GFAQMRKASEDNEWNLKLGDLAMIWREGCIIRAQFLQKIKDAYDNNPGLQNLLLDPYF
                     KNIVTEYQDALRDVVATGVQNGVPTPGFSSSINYYDSYRAADLPANLIQAQRDYFGAH
                     TYERKDKEGVFHTQWIEE"
BASE COUNT          991 a          390 c          572 g          857 t
ORIGIN      
        1 taaatctgtg taacgtcaat tactgtaaca ttttatttca tcaattttta ccgtttggcg
       61 tacttgaaaa ttttgataaa aggggttcca ttatgattaa tgagcaaaga ttacttaata
      121 cgtttttaga acttgtacaa atcgattctg agacagggaa tgaatcaaca atccaaccta
      181 ttttaaaaga aaaattcata gcattagggt tagatgttaa agaagatgaa gcggctaaac
      241 atcctaaatt aggtgccaat aatctagtgt gtacaatgaa tagtactatc gaagaaggcg
      301 aagtacctaa attatatttg acgagccata tggatactgt tgtacccgca attaatgtaa
      361 agccaattgt aaaagatgac ggctacatat actctgatgg tacgactatt ctaggtgcag
      421 atgataaagc aggattagca gcgatgcttg aagttttgca agtgataaag gaacaacaaa
      481 tcccacatgg acaaattcaa tttgtgatta ctgttgggga agaatcaggt ttaattggtg
      541 ctaaagaatt aaattcagag ttgttggacg cggactttgg ttatgctatt gatgcaagtg
      601 ctgatgtcgg tactacggtt gtaggtgcac cgacgcaaat gttaatttca gctaaaatta
      661 ttggcaaaac agctcatgca agtacgccaa aagagggtgt tagtgctatt aatattgcgg
      721 ctaaagcaat tagtcgaatg aaattaggac aagttgatga aataactact gccaatattg
      781 gtaagtttca tggcggttca gctacgaata ttgttgcaga tgaagtcatt ttagaagcag
      841 aggcacgttc tcacgatcca gaaagaataa aaacacaagt caaacatatg actgatgtgt
      901 ttgaaactac tgcatcagaa ttaggtggga aagcagaagt tacagtggag caaagttatc
      961 caggatttaa aattaatgat aatgaagcgg ttgttaaaat tgcacaagaa agtgctcgta
     1021 atttaggatt atctgcaaat acaattattt ctggtggcgg ttcagacggc agtattatta
     1081 atacatttgg tattccttca gtcattttag gagtgggtta tgaaaaaatc catacaacaa
     1141 atgagagaat gccgattaaa tcattgaact tgcttgcaag tcaagtgtta gaaataatca
     1201 aaatagttgc gcgacaatcg aaatgattta gtgtagtgta tgtctatttt gtgatacaat
     1261 actaatgaaa aatcaacaag tgaggtaagt aatatgacac aacaaattgg agttattggt
     1321 ttagccgtta tgggtaaaaa cctagcttgg aatattgaat cacgcggata tagtgtatct
     1381 gtatttaacc gctcaagtga aaaaactgat ttaatggttg aagaatcaaa agggaaaaat
     1441 attcatccaa cgtattcatt ggaagaattt gttaattctt tagaaaaacc acgtaaaatt
     1501 ttattaatgg ttcaagcagg taaagctacg gatgcaacaa ttgatagttt gttaccttta
     1561 ttagatgatg gagatatttt aattgatggt ggtaatacaa actatcaaga tactatcaga
     1621 cgtaataaag cattagcaca aagtgcaatc aactttattg gtatgggcgt ttctggtggt
     1681 gaaattggag cgttaactgg tccgtcatta atgccgggtg gacaagaaga agcttataac
     1741 aaagttgcag atattcttga tgcaattgct gctaaggcaa aagacggtgc ttcatgtgtt
     1801 acttatattg gaccaaatgg tgctggacac tatgtgaaaa tggtacataa tggtattgaa
     1861 tatgctgaca tgcaattaat tgcagaaagt tatgctatga tgaaagaatt attaggtatg
     1921 tctcatgaag acattgctca aacgttcaaa gactggaatg ctggtgaatt agaaagttat
     1981 ttaattgaaa tcactggtga tattttcatg aaattagatg aaaataaaga agcattagtt
     2041 gaaaaaattc ttgatactgc aggtcaaaaa ggtacaggaa aatggacttc aattaatgca
     2101 ttagaattag gtattccatt aacaatcatt actgaatctg tatttgcacg ttttatctct
     2161 tcaatcaaag aagagcgcgt aaatgcttct aaagaattaa atggtccaaa agcatcattt
     2221 gatggtgaca agaaagactt tttagaaaaa attcgtaaag cattatatat gagtaaaatt
     2281 tgttcttatg cacaaggttt cgcgcaaatg cgtaaagcaa gtgaagataa tgaatggaat
     2341 ttaaaacttg gtgatttagc tatgatttgg agagaaggtt gcattatacg tgcacaattc
     2401 ttacaaaaga tcaaagatgc ttacgataat aatccaggat tacaaaactt attgctagat
     2461 ccttacttta aaaacatcgt tactgaatat caagatgcgt tacgtgatgt tgtcgcaact
     2521 ggtgttcaaa atggtgtacc aacacctggt ttctcatcaa gtattaatta ctatgatagt
     2581 taccgtgcag ctgatttacc tgcaaactta attcaagcac aacgtgacta cttcggtgct
     2641 cacacgtacg aaagaaaaga taaagaaggc gtattccata cacaatggat tgaagaataa
     2701 tattgaccat tgagcctgag accttatgaa tttaggtaat gttaaagaga caatttctat
     2761 tatttatttg atagaaattg tctctttcct tatgtattta tcgaaatttt
//
LOCUS       DAHHSC010000246         2808 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.250, whole genome shotgun sequence.
ACCESSION   DAHHSC010000246 DAHHSC010000000
VERSION     DAHHSC010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2808)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2808)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2808
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.250"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            46..2577
                     /gene="secA"
                     /locus_tag="NBI57_002460"
     CDS             46..2577
                     /gene="secA"
                     /locus_tag="NBI57_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HCD1823382.1"
                     /translation="MGFLSKILDGNNKEIKQLGKLADKVIALEEKTAILTDEEIRNKT
                     KQFQTELADIDNVKKQNDYLDKILPEAYALVREGSKRVFNMTPYKVQIMGGIAIHKGD
                     IAEMRTGEGKTLTATMPTYLNALAGRGVHVITVNEYLSSVQSEEMAELYNFLGLTVGL
                     NLNSKTTEEKREAYAQDITYSTNNELGFDYLRDNMVNYSEDRVMRPLHFAIIDEVDSI
                     LIDEARTPLIISGEAEKSTSLYTQANVFAKMLKQDEDYKYDEKTKAVHLTEQGADKAE
                     RMFKVENLYDVQNVDVISHINTALRAHVTLQRDVDYMVVDGEVLIVDQFTGRTMPGRR
                     FSEGLHQAIEAKEGVQIQNESKTMASITFQNYFRMYNKLAGMTGTAKTEEEEFRNIYN
                     MTVTQIPTNKPVQRNDKSDLIYISQKGKFDAVVEDVVEKHKAGQPVLLGTVAVETSEY
                     ISNLLKKRGIRHDVLNAKNHEREAEIVAGAGQKGAVTIATNMAGRGTDIKLGEGVEEL
                     GGLAVIGTERHESRRIDDQLRGRSGRQGDKGDSRFYLSLQDELMIRFGSERLQKMMSR
                     LGLDDSTPIESKMVSRAVESAQKRVEGNNFDARKRILEYDEVLRKQREIIYNERNSII
                     DEEDSSQVVDAMLRSTLQRSINYYINTADDEPEYQPFIDYINDIFLQEGDITEDDIKG
                     KDAEDIFEVVWAKIEAAYQSQKDILEEQMNEFERMILLRSIDSHWTDHIDTMDQLRQG
                     IHLRSYAQQNPLRDYQNEGHELFDIMMQNIEEDTCKFILKSVVQVEDNIEREKTTEFG
                     EAKHVSAEDGKEKVKPKPIVKGDQVGRNDDCPCGSGKKFKNCHGK"
     gene            2619..2723
                     /locus_tag="NBI57_002461"
     CDS             2619..2723
                     /locus_tag="NBI57_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1823383.1"
                     /translation="MLWEESFYFTSVKYFKKCEEVVKRCYVLSFKKSV"
BASE COUNT         1036 a          405 c          561 g          806 t
ORIGIN      
        1 tattgttgta ggccaaacag ttttttagct aaaggagcga acgaaatggg atttttatca
       61 aaaattcttg atggcaataa taaagaaatt aaacagttag gtaaacttgc tgataaagta
      121 atcgctttag aagaaaaaac ggcaatttta actgatgaag aaattcgtaa taaaacgaaa
      181 caattccaaa cagaattagc tgacattgat aatgtcaaaa agcaaaatga ttatttagat
      241 aaaattttac cagaagcata tgcacttgtt agagaaggct ctaaacgtgt attcaatatg
      301 acaccatata aagttcaaat tatgggtggt attgcaattc ataaaggtga tatcgctgag
      361 atgagaacag gtgaaggtaa aacattaaca gcgacaatgc caacatactt aaatgcatta
      421 gctggtagag gtgttcacgt tattacagtc aatgaatact tatcaagtgt tcaaagtgaa
      481 gaaatggctg agttatataa cttcttaggt ttgactgtcg gattaaactt aaacagtaag
      541 acgacagaag aaaaacgtga agcatacgca caagacatta cttacagtac taataatgag
      601 ctaggttttg attacttacg agataacatg gtgaattatt ctgaagatag agtaatgcgt
      661 ccattacatt ttgcaatcat tgatgaggtt gactcaattt taatcgacga ggcacgtacg
      721 ccattaatta tttctggtga agctgaaaag tcaacgtcac tttatacaca agcaaatgtt
      781 tttgcgaaaa tgttaaaaca ggacgaagat tataaatacg atgaaaaaac gaaagccgta
      841 catttaacag aacaaggtgc ggataaagct gaacgtatgt tcaaagttga aaacttatat
      901 gatgtacaaa atgttgatgt tattagtcat atcaacacag ctttacgtgc gcacgttaca
      961 ttacaacgtg acgtagacta tatggttgtt gatggcgaag tactaattgt cgatcaattt
     1021 acaggacgta caatgccagg ccgtcgtttc tcagaaggtt tacaccaagc tattgaagcg
     1081 aaggaaggcg ttcaaattca aaatgaatct aaaactatgg cgtctattac attccaaaac
     1141 tatttcagaa tgtacaataa acttgcgggt atgacaggta cagctaaaac tgaagaagaa
     1201 gaatttagaa atatttataa catgacagta actcaaattc cgacaaataa acctgtgcaa
     1261 cgtaacgata agtctgattt aatttacatt agccaaaaag gtaaatttga tgcagtagta
     1321 gaagatgttg ttgaaaaaca caaggcaggg caaccagtgc tattaggtac tgttgcagtt
     1381 gagacttctg aatatatttc aaatttactt aaaaaacgtg gtatccgtca tgatgtgtta
     1441 aatgcgaaaa atcatgaacg tgaagctgaa attgttgcag gcgctggaca aaaaggtgcc
     1501 gttactattg ccactaacat ggctggtcgt ggtacagata tcaaattagg tgaaggcgta
     1561 gaggaattag gcggtttagc agtaataggt acagaacgac atgaatctcg tcgtattgat
     1621 gaccagttac gtggtcgttc tggacgtcaa ggtgataaag gggatagtcg cttctattta
     1681 tcattacaag atgaattaat gattcgtttt ggttctgaac gtttacagaa aatgatgagc
     1741 cgactaggtt tagatgactc tacaccaatt gaatcaaaaa tggtatcaag agctgtagaa
     1801 tcagcacaaa aacgtgtaga aggtaataac ttcgacgcgc gtaaacgtat cttagaatac
     1861 gatgaagtat tacgtaaaca acgtgaaatt atctataacg aaagaaatag tattattgat
     1921 gaagaagaca gctctcaagt tgtagatgca atgctacgtt caacgttaca acgtagtatc
     1981 aattactata ttaatacagc agatgacgag cctgaatatc aaccattcat cgactacatt
     2041 aatgacattt tcttacaaga aggtgacatt acagaggatg atatcaaagg taaagatgct
     2101 gaagatattt tcgaagtcgt ttgggctaag attgaagcag catatcaaag tcaaaaagat
     2161 atcttagaag aacaaatgaa tgagtttgag cgtatgattt tacttcgttc tattgatagc
     2221 cattggactg atcatatcga cacaatggat caattacgtc aaggtattca cttacgttct
     2281 tatgcacagc aaaatccatt acgtgactat caaaatgaag gtcatgaatt atttgatatc
     2341 atgatgcaaa atatcgaaga agatacttgt aaattcattt taaaatctgt agtacaagtt
     2401 gaagataata ttgaacgtga aaaaacaaca gagtttggtg aagcgaagca cgtttcagct
     2461 gaagatggta aagaaaaagt gaaaccgaaa ccaatcgtta aaggcgatca agttggtcgt
     2521 aacgatgatt gtccatgtgg tagtggtaaa aaattcaaaa attgccatgg aaaataaatg
     2581 atataaaata actccttcca attaaacacc tatagtttgt gttatgggag gagtcttttt
     2641 attttacaag cgttaaatac tttaaaaaat gtgaagaagt tgttaaacgt tgttatgtac
     2701 ttagttttaa aaaatcggtt taggcatatg tcgatgataa atgtactgat ttttaacaat
     2761 aaaagcataa actaattgtc agtgtgctta tatttcttaa cattgtta
//
LOCUS       DAHHSC010000247         2771 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.251, whole genome shotgun sequence.
ACCESSION   DAHHSC010000247 DAHHSC010000000
VERSION     DAHHSC010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2771)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2771)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2771
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.251"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            366..875
                     /locus_tag="NBI57_002462"
     CDS             366..875
                     /locus_tag="NBI57_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCD1823384.1"
                     /translation="MIRLGKMSDLDQILNLVEEAKELMKEHDNEQWDDQYPLLEHFEE
                     DIAKDYLYVLEENDKIYGFIVVDQDQAEWYDDIDWPVNREGAFVIHRLTGSKEYKGAA
                     TELFNYVIDVVKARGAEVILTDTFALNKPAQGLFAKFGFHKVGEQLMEYPPYDKGEPF
                     YAYYKNLKE"
     gene            887..1957
                     /locus_tag="NBI57_002463"
     CDS             887..1957
                     /locus_tag="NBI57_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HCD1823385.1"
                     /translation="MTKIAFTGGGTVGHVSVNLSLIPTALSQGYEALYIGSKNGIERE
                     MIESQLPEIKYYPISSGKLRRYISLENAKDVFKVLKGILDARKVLKKEKPDLLFSKGG
                     FVSVPVVIAAKSLNIPTIIHESDLTPGLANKIALKFAKKIYTTFEETLNYLPKEKADF
                     IGATIREDLKNGNAHNGYQLTGFNENKKVLLVMGGSLGSKKLNSIIRENLDALLQQYQ
                     VIHLTGKGLKDAQVKKSGYIQYEFVKEDLTDLLAITDTVISRAGSNAIYEFLTLRIPM
                     LLVPLGLDQSRGDQIDNANHFADKGYAKTIDEEQLTAQILLQELNEMEQERTRIINNM
                     KSYEQSYTKEALFDKMIKDALN"
     gene            1974..2588
                     /locus_tag="NBI57_002464"
     CDS             1974..2588
                     /locus_tag="NBI57_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HCD1823386.1"
                     /translation="MSQWKRISLLIVFALVFGIIAFFHESRLGKWIDNEVYEFIYSSE
                     SFITTSIMLGATKVGEVWAMLCISLLLVAYLMLKRHKIEALFFALTMALSGILNPALK
                     NIFDRERPTLLRLIDITGFSFPSGHAMGSTAYFGSGIYLLNRLNQGNSKGILIGLCAA
                     MILLISISRVYLGVHYPTDIIAGIIGGVFCIILSTLLLRNKLIN"
BASE COUNT         1010 a          359 c          501 g          901 t
ORIGIN      
        1 gttaatgaca cgcttcctaa atttgcaaag atagcaattt aggaggcgtg tttattttta
       61 ttgacgtcta accctaaaag atataaatta gacatttaca aatgatgtaa ataacgcaat
      121 ttctatcatc gctgttaaca attcatgctt taatatacta tgagcatata ctttttaaat
      181 agtattattc actagtttta acaatcgatt aattggtata tgatactttt attggttatt
      241 ttcatcccat agtgtgataa ttactatttt tcattcataa taaaggttta aagcatgtta
      301 atagtgtgta agattaacat gtactgaaaa acatgtttaa cataacgata taaggagtga
      361 cgtacatgat ccgtctaggt aaaatgtcag atttagatca aatcttaaat ctagtagaag
      421 aagcaaaaga attaatgaaa gaacacgaca acgagcaatg ggacgatcag tacccacttt
      481 tagaacattt tgaagaagat attgctaaag attatttgta cgtattagag gaaaatgaca
      541 aaatttatgg ctttattgtt gtcgaccaag accaagcaga atggtatgat gacattgact
      601 ggccagtaaa tagagaaggc gcctttgtta ttcatcgatt aactggttcg aaagaatata
      661 aaggagctgc tacagaatta ttcaattatg ttattgatgt agttaaagca cgtggtgcag
      721 aagttatttt aacggacacc tttgcgttaa acaaacctgc acaaggttta tttgcgaaat
      781 ttggatttca taaggtcggt gaacaattaa tggaatatcc gccatatgat aaaggtgaac
      841 cattttatgc atattataaa aatttaaaag aatagaggta atattaatga cgaaaatcgc
      901 atttaccgga gggggaacag ttggacacgt atcagtaaat ttaagtttaa ttccaactgc
      961 attatcacaa ggttatgaag cgctttatat tggttctaaa aatggtattg aaagagaaat
     1021 gattgaatca caactaccag aaattaagta ttatcctatt tcgagtggta aattaagaag
     1081 atatatttct ttagaaaatg ccaaagacgt atttaaagta ttgaaaggta ttcttgatgc
     1141 tcgtaaagtt ttgaaaaaag aaaaacctga tctattattt tcaaaaggtg gatttgtatc
     1201 tgtgcctgtt gttattgcag ccaaatcatt aaatatacca actattattc atgaatctga
     1261 cttaacacca ggattagcga ataagatagc acttaaattt gccaagaaaa tatatacaac
     1321 atttgaagaa acgctaaact acttacctaa agagaaagct gattttattg gagcaacaat
     1381 tcgagaagat ttaaaaaatg gtaatgcaca taatggttat caattaacag gctttaatga
     1441 aaataaaaaa gttttacttg ttatgggtgg aagcttagga agtaaaaaat taaatagcat
     1501 tattcgcgaa aacttagatg cattattaca acaatatcaa gtgatacatt taactggtaa
     1561 aggattaaaa gatgctcaag ttaaaaaatc aggatatata caatatgaat ttgttaaaga
     1621 ggatttaaca gatttattag caattacgga tacagtaata agtagagctg gatcaaatgc
     1681 gatttatgag ttcttaacat tacgtatacc aatgttatta gtaccattag gtttagatca
     1741 atcccgaggc gaccaaattg acaatgcaaa tcattttgct gataaaggtt atgctaaaac
     1801 gattgatgaa gaacaattaa cagcacaaat tttattacaa gaactaaatg aaatggaaca
     1861 ggaaagaact cgaattatca ataatatgaa atcgtatgaa caaagttata cgaaagaagc
     1921 tttatttgat aagatgatta aagacgcatt gaattaatgg ggggtaatgc tttatgagtc
     1981 aatggaaacg tatctctttg ctcatcgttt ttgcattggt ttttggaatt atcgcgtttt
     2041 tccacgaatc aagactaggg aaatggattg ataatgaagt ttatgaattt atatattcat
     2101 cagagagctt tattacgaca tctatcatgc ttggggctac taaagtaggt gaagtctggg
     2161 caatgttatg tatttcatta cttcttgtgg catatctcat gttaaagcgc cacaaaattg
     2221 aagcattatt ttttgcatta acaatggcac tatctggaat tttgaatcca gcattaaaaa
     2281 atatattcga tagagaaaga cctacattgc tgcgtttaat tgatataaca ggatttagtt
     2341 ttcctagcgg tcatgctatg ggatcaactg catattttgg aagtggtatc tatctattaa
     2401 atcgattaaa tcaaggtaat tcaaaaggta ttcttatagg gttatgtgca gctatgattt
     2461 tattgatttc catatcacgt gtatatctag gtgtacatta tccaacagat attattgccg
     2521 gcattattgg tggcgtattt tgcattattt tatcaacgtt attacttaga aataaattaa
     2581 taaattaaat agtaaaaaaa caaaagcagt aaacctaaag tgtcgtaagg gtttactgct
     2641 tttataaaac gttgttataa cgtatattgt cttttacggg catataaaag gggaatattt
     2701 gaaaatgacc aatccaacaa gaacgaaacg ttgtgggggg gatgttctat gtggtattga
     2761 taatcatttt c
//
LOCUS       DAHHSC010000248         2767 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.252, whole genome shotgun sequence.
ACCESSION   DAHHSC010000248 DAHHSC010000000
VERSION     DAHHSC010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2767)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2767)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2767
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.252"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            complement(136..1395)
                     /gene="femB"
                     /locus_tag="NBI57_002465"
     CDS             complement(136..1395)
                     /gene="femB"
                     /locus_tag="NBI57_002465"
                     /EC_number="2.3.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine glycyltransferase FemB"
                     /protein_id="HCD1823387.1"
                     /translation="MKFTELTVTEFDNFVQNPSLESHYFQVKENIVTRENDGFEVVLL
                     GIKDDNNKVIAASLFSKIPTMGSYVYYSNRGPVMDFSDLGLVDYYLKELDKYLQQHQC
                     LYVKLDPYWLYHLYDKDIVPFEGREKNDALVNLFKSHGYEHHGFTTEYDTSSQVRWMG
                     VLNLEGKTPETLKKTFDSQRKRNINKAINYGVKVRFLERDEFNLFLDLYRETEERAGF
                     VSKTDDYFYNFIDTYGDKVLVPLAYIDLDEYVLKLQQELNDKENRRDQMMAKENKSDK
                     QMKKIAELDKQIDHDQHELLNASELSKTDGPILNLASGVYFANAYEVNYFSGGSSEKY
                     NQFMGPYMMHWFMINYCFDNGYDRYNFYGLSGDFTENSEDYGVYRFKRGFNVQIEELI
                     GDFYKPIHKVKYWLFTTLDKLRKKLKK"
     gene            complement(1414..2676)
                     /gene="femA"
                     /locus_tag="NBI57_002466"
     CDS             complement(1414..2676)
                     /gene="femA"
                     /locus_tag="NBI57_002466"
                     /EC_number="2.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine glycyltransferase FemA"
                     /protein_id="HCD1823388.1"
                     /translation="MKFTNLTAKEFGAFTDSMPYSHFTQTVGHYELKLAEGYETHLVG
                     IKNNNNEVIAACLLTAVPVMKVFKYFYSNRGPVIDYENQELVHFFFNELSKYVKKHRC
                     LYLHIDPYLPYQYLNHDGEITGNAGNDWFFDKMSNLGFEHTGFHKGFDPVLQIRYHSV
                     LDLKDKTADDIIKNMDGLRKRNTKKVKKNGVKVRYLSEEELPIFRSFMEDTSESKAFA
                     DRDDKFYYNRLKYYKDRVLVPLAYINFDEYIKELNEERDILNKDLNKALKDIEKRPEN
                     KKAHNKRDNLQQQLDANEQKIEEGKRLQEEHGNELPISAGFFFINPFEVVYYAGGTSN
                     AFRHFAGSYAVQWEMINYALNHGIDRYNFYGVSGKFTEDAEDAGVVKFKKGYNAEIIE
                     YVGDFIKPINKPVYAAYTALKKVKDRIF"
BASE COUNT          883 a          503 c          363 g         1018 t
ORIGIN      
        1 tcattataat gaaaaacatt agattgacaa atacatacta gtttttctta gtgtgaaata
       61 attaaaattt cgctaaaaaa agcataaaaa agagcctata gtatcaataa ctataagccc
      121 taacatcatt tacatctatt tctttaattt tttacgtaat ttatccaatg ttgtgaacaa
      181 ccaatatttc actttatgaa ttggtttata gaaatcccct attaattctt cgatttgtac
      241 attaaatcca cgtttaaagc ggtatacgcc ataatcttca ctgttttccg taaaatcacc
      301 tgataaacca tagaaattat aacgatcata gccattatcg aagcaatagt taatcataaa
      361 ccaatgcatc atgtatggtc ccataaattg attatatttt tctgatgaac caccagagaa
      421 ataattcact tcatatgcat ttgcaaaata aacgccagaa gcaaggttta gaattgggcc
      481 gtccgttttg ctcaattcac ttgcattcaa taattcatgc tgatcatgat caatttgctt
      541 atctaattct gcaattttct tcatttgttt atctgatttg ttttctttcg ccatcatttg
      601 atcacgacga ttttctttgt cattcaattc ctgttgcaac tttaacacat attcatcaag
      661 gtcaatatat gctaatggta ctaatacttt atctccatat gtgtcaataa agttataaaa
      721 ataatcatct gtttttgaca caaatccagc acgctcttca gtttcacgat ataaatctaa
      781 gaaaagattg aactcatcac gttcaaggaa tctgacttta acaccatagt ttatcgcttt
      841 attaatatta cgtttacgtt gactatcaaa tgtctttttc aatgtttcgg gtgttttacc
      901 ttcaaggttt aatacgccca tccatcgtac ttggctcgat gtatcatact cagttgtaaa
      961 gccatgatgc tcgtaaccat gtgatttaaa caagtttact aaggcatcat ttttctcgcg
     1021 accttcaaat ggcacgatat ctttatcata tagatgatat aaccaatacg gatctaattt
     1081 aacatataaa cattgatgtt gctgtaaata tttatctaac tcttttaaat aataatcaac
     1141 taatcctaaa tctgaaaaat ccattactgg accacgattc gaatagtaaa cataacttcc
     1201 catagtagga attttagaga aaaggcttgc tgcaattact ttgttattgt cgtctttaat
     1261 acctaataaa actacttcaa agccatcatt ctcacgggta actatatttt cttttacttg
     1321 gaaataatga ctttccaatg atggattttg tacaaagttg tcaaattcgg taacagttaa
     1381 ctctgtaaat ttcatgtttt gataattccc ttcctaaaaa attctgtctt taactttttt
     1441 aagtgcggta tatgctgcgt aaacaggttt attaattggt ttaataaagt caccaacata
     1501 ttcaataatt tcagcattgt aacctttttt gaatttaact acaccagcat cttcagcatc
     1561 ttctgtaaat ttaccactaa caccatagaa attataacgg tcaatgccat gatttaatgc
     1621 ataattaatc atttcccatt gcactgcata acttccggca aaatgacgga atgcatttga
     1681 tgtaccacca gcataataaa caacttcaaa tgggttgata aagaagaaac cagcagagat
     1741 aggtaattca ttaccatgtt cttcttgtag acgtttacct tcttcaatct tttgctcatt
     1801 agcatcaagt tgttgttgta agttatctcg cttgttgtgt gcttttttat tttcaggacg
     1861 tttttcaata tcctttaacg ctttatttaa atctttattt aaaatatcac gctcttcgtt
     1921 tagttcttta atatattcat caaagttgat atacgctaaa ggtactaaca cacggtcttt
     1981 gtaatatttt aagcgattgt agtaaaattt gtcatcacga tcagcaaaag cttttgattc
     2041 tgacgtatct tccataaatg atctaaaaat tggcagttct tcttcagata aatatcttac
     2101 tttaacacca ttctttttaa cttttttcgt gtttcttttt ctaagtccat ccatattttt
     2161 aatgatgtca tctgctgttt tatcttttaa atctaacact gagtgataac gaatttgtag
     2221 cacaggatca aatcctttat ggaatccagt atgttcaaat cctaagttac tcattttatc
     2281 aaagaaccaa tcattaccag cattacctgt aatctcgcca tcatgattca agtattgata
     2341 tggtaaatat ggatcgatat gtaggtatag acaacgatgt tttttaacat attttgataa
     2401 ttcattaaag aaaaagtgta cgagttcttg attttcataa tcgatcactg gaccgcgatt
     2461 tgaataaaaa tacttgaaca ctttcataac aggtacagca gtaagtaagc aagctgcaat
     2521 gacctcgtta ttattgtttt ttattcccac taaatgtgtt tcataacctt cagcaagctt
     2581 taactcatag tggccaacag tttgcgtgaa atgactgtat ggcatgctat ctgtaaaggc
     2641 accaaactct ttagctgtta aatttgtaaa cttcattatc attactccta tttgtctctc
     2701 gttaattaat ttcatttccg tatttgcagt ttttctattt cccctctgca aatgtcaaaa
     2761 ataataa
//
LOCUS       DAHHSC010000249         2764 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.253, whole genome shotgun sequence.
ACCESSION   DAHHSC010000249 DAHHSC010000000
VERSION     DAHHSC010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2764)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2764)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2764
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.253"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            <1..594
                     /locus_tag="NBI57_002467"
     CDS             <1..594
                     /locus_tag="NBI57_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000284214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEA family DNA-binding protein"
                     /protein_id="HCD1823389.1"
                     /translation="LIGFIFWRQDDYTSRNFENKDTALKQSTSENNSLSKLEDVQVKD
                     GDNSKNKGPVYVDVKGAVKHPNVYKMTSKDRVVDLLDKAQLLDDADVSRINLSEKLTD
                     QKMIFIPHKGQKNVEPQIEVNSVHVKNGNTNNTKVNLNTASVSELMSVPGVGQAKANA
                     IVEYRNQQGAFQEIDDLKKVKGFGSKTFDKLKSYFTI"
     gene            686..1147
                     /locus_tag="NBI57_002468"
     CDS             686..1147
                     /locus_tag="NBI57_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000439692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComE operon protein 2"
                     /protein_id="HCD1823390.1"
                     /translation="MERIKWEEYFMAQSHLLALRSTCQRLSVGATIVKDNRIIAGGYN
                     GSVASEVHCIDEGCLIEDGHCIRTIHAEMNALLQCAKQGVSTEGATIYVTHFPCLNCT
                     KSIIQAGIKRIYYAEDYHNHEYATKLLKQSGIEFKKIPFSPEYVAKYLTKG"
     gene            1152..>2764
                     /locus_tag="NBI57_002469"
     CDS             1152..>2764
                     /locus_tag="NBI57_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001837660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HCD1823391.1"
                     /translation="MLYVALSMIVGVLWNSSKVLSTFLFILLLYITYRKNKIVYAPIS
                     LFLIIFSSWYLHYSQQAIFNYINYIERNSQFNERAQVIQIQRQGSDTYKGRLSLKNEI
                     YPFFLTDKKNFDLKKIESRNCIVKGQFKVNENKFVTLKLQSIVVQSCLESNRSNLIEK
                     HKQFIMNRIYDSGIKFPDRIMALITGDVKEINEQFKERVKEIGIYHLLAVSGSHIAAI
                     VFLIYQPLKRLNLPLFVIKGITIIVLTLYAQYTNYAPSAVRAIIMITLVLLITKQIKI
                     KGIQLLAFAFIIMFILNPLVVYDIGFQFSFIISFFIMLLFPFLQQLSKLQSLFIITFI
                     AQLASFIVAIPNFHQLQWVGFLSNLIFVPYYSIILFPLSILFFITSHFIVGLTPLNYL
                     VDLSFNFHDWLLDLFTRIKQSHFSVPKFNDWIFIIFIISVYYIFWLLAKRKYILVTFW
                     TIIILTLLITFPTNSHHKITMLNVGQGDSILYEGGKNQNVLIDTGGKVIDDTKQPSYS
                     ISKYHILPTLNERGINELEYLILTHPHND"
BASE COUNT          969 a          371 c          441 g          983 t
ORIGIN      
        1 ttaattggtt ttatattctg gagacaagat gattatactt caagaaattt tgaaaataaa
       61 gatactgctc tgaaacaaag cactagtgaa aataatagtt tgtccaaatt agaagatgtc
      121 caggtcaaag atggagataa ttccaaaaat aaaggtcctg tatatgtcga tgtaaaaggt
      181 gctgttaaac atcctaatgt ttataaaatg acatctaagg atagagtagt tgatttactt
      241 gataaagcac aattattgga tgatgcagat gtaagtcgaa ttaatttgtc tgaaaaatta
      301 acagatcaaa aaatgatttt catacctcat aaaggacaaa agaatgttga accacaaatt
      361 gaagtaaaca gtgtgcacgt aaaaaatggg aacacaaata atactaaagt aaatttaaat
      421 accgcatctg tatcagaatt gatgtctgtt cctggagtag ggcaagctaa agctaatgca
      481 attgttgaat atcgcaacca acaaggtgca tttcaagaaa ttgacgattt gaaaaaagta
      541 aaaggttttg gaagtaaaac ttttgataaa ctgaaatctt atttcacgat ataatggatt
      601 attttaattg caaattttat tttttgtgat taatcttata ttattagaaa atgttactaa
      661 attttaagta tggaaggtga acatcttgga aagaatcaaa tgggaagaat attttatggc
      721 acaaagtcat ttgctagcat tacgttcaac ttgtcaaaga ttatctgtag gtgcaacgat
      781 tgttaaggat aatcgtatta ttgctggtgg ttataatggc tctgtagcta gcgaggtgca
      841 ttgtatagat gaaggatgtt taattgaaga tggacattgt atcagaacga tacatgcaga
      901 aatgaatgct ttattacaat gtgcaaaaca aggtgtatct actgaaggtg caacaatcta
      961 tgttactcat tttccatgcc taaattgtac aaagtcaatt attcaagcag gtataaagcg
     1021 tatctactat gcagaagatt atcataacca tgaatatgca actaaattac tcaaacaatc
     1081 tggtattgaa tttaaaaaaa ttccattttc accagaatat gttgctaaat atctgactaa
     1141 aggttaatgt tttgctgtat gtcgcgttat caatgattgt aggagtgctt tggaattcta
     1201 gcaaagtgct ctctacattt cttttcattt tacttttgta tattacttat cgtaaaaata
     1261 aaatcgttta tgcccctatt tctctctttt taatcatttt ctcctcatgg tatttacatt
     1321 attcacaaca agcaatattt aattatatca attatattga acgtaattct cagtttaatg
     1381 agcgtgctca agtaatccaa attcaacgtc aaggtagtga cacatataaa ggtaggttga
     1441 gtttaaaaaa tgaaatatat cctttctttt taacagataa aaagaatttt gatttaaaga
     1501 aaattgaaag tcgtaattgt attgttaaag gacaattcaa agttaatgaa aataagtttg
     1561 taactcttaa attacaaagt atagttgtac aaagctgcct agaatcgaac cggtctaatt
     1621 taattgagaa acataaacag tttataatga atcgaattta tgattcgggt attaagtttc
     1681 cggatcgtat tatggcattg attactggtg acgtaaaaga aattaatgag caatttaagg
     1741 aacgtgttaa agagataggt atatatcatt tgctggcagt tagtggctcg catatagctg
     1801 caattgtatt cttaatttac caacctttaa aacgattaaa tttaccttta tttgtcatta
     1861 aaggaattac aatcattgta ttaactttat atgctcaata cacaaattat gcacctagtg
     1921 ctgtaagagc tataataatg ataactcttg tactgcttat tactaagcaa attaaaataa
     1981 agggtattca gctattagca tttgcattta taattatgtt tattttaaac ccactagttg
     2041 tttatgatat tggatttcaa ttttcattca tcatttcatt ttttattatg ctactttttc
     2101 cttttttaca gcaattgtca aagttacaat cattattcat aattacgttt attgcacaat
     2161 tagcttcatt tatcgttgcc attccaaact ttcatcaact tcaatgggtg ggatttttat
     2221 ctaatttgat ttttgtaccg tactattcga ttatattgtt tccgctatct attttattct
     2281 ttattacaag tcattttatt gtgggattaa cgccgctaaa ttacttggtt gacctaagtt
     2341 ttaattttca tgactggtta ctagacctat tcacaagaat taagcaatca catttttctg
     2401 ttcccaagtt taatgattgg atatttataa tatttataat ttctgtttat tacatatttt
     2461 ggttattggc taaacgtaaa tatatattgg ttacgttttg gactataatt attctgacat
     2521 tattaataac gtttccaaca aattcacatc acaaaattac aatgttaaat gtggggcagg
     2581 gagacagtat tttatatgaa ggcggtaaga accaaaatgt cttgattgat acaggtggga
     2641 aagtgattga tgatactaaa caacctagtt attcaatttc taaatatcat attttaccaa
     2701 cgctaaatga aagagggata aatgaattag agtatctaat tttaacacat ccacacaatg
     2761 acca
//
LOCUS       DAHHSC010000250         2753 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.254, whole genome shotgun sequence.
ACCESSION   DAHHSC010000250 DAHHSC010000000
VERSION     DAHHSC010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2753)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2753)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2753
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.254"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            complement(<1..265)
                     /locus_tag="NBI57_002470"
     CDS             complement(<1..265)
                     /locus_tag="NBI57_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3055 domain-containing protein"
                     /protein_id="HCD1823392.1"
                     /translation="MIDMYLYDDNEESQVQFVGFVGEHSRYDLMLVHTNRHYGKTLVL
                     NMQTNKFGIIGTDDLKEEGYIAHILGVNAEEGDEITEYLNEVIH"
     gene            363..797
                     /locus_tag="NBI57_002471"
     CDS             363..797
                     /locus_tag="NBI57_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF86 domain-containing protein"
                     /protein_id="HCD1823393.1"
                     /translation="MYFVDKDKLTQKLAYLQALTDDYHESKHNHYAFERIAQMLIESS
                     VDIGNMIIDAFILRDPGNYKDVIDILELENVITKETQQEINKTVDIRKQFTYDYTALD
                     IKVIMPMFDDALPYYKQFITEVTIFLHQENVPVTAFGKGENQ"
     gene            797..1576
                     /locus_tag="NBI57_002472"
     CDS             797..1576
                     /locus_tag="NBI57_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HCD1823394.1"
                     /translation="MKQYKAYLIDLDGTMYMGTDEIDGAKQFIDYLNVKGIPHLYVTN
                     NSTKTPEQVTEKLREMHIDAKPEEVVTSALATADYISEQSPGASVYMLGGSGLNTALT
                     EAGLVIKNDEHVDYVVIGLDEQVTYEKLAIATLGVRNGATFISTNPDVSIPKERGLLP
                     GNGAITSVVSVSTGVSPQFIGKPEPIIMVKALEILGLDKSEVAMVGDLYDTDIMSGIN
                     VGMDTIHVQTGVSTLEDVQNKNVPPTYSFKDLNEAIAELEK"
     gene            1609..2568
                     /locus_tag="NBI57_002473"
     CDS             1609..2568
                     /locus_tag="NBI57_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000239136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycerate dehydrogenase"
                     /protein_id="HCD1823395.1"
                     /translation="MVKIVVSRKIPDKFYQQLSKLGDVVMWQKSLVPMPKDQFVTALR
                     DADACFITLSEQIDAEILAQSPNLKVIANMAVGYDNIDVESATANNVVVTNTPNVLTE
                     TTAELGFTLMLAIARRIVEAEKYVEADAWQSWGPYLLSGKDVFNSTIGIYGMGDIGKA
                     FARRLQGFNTNILYHNRSRHKDAEADFNATYVSFETLLAESDFIICTAPLTKETHHKF
                     NAEAFEQMKNDAIFINIGRGQIVDETALIDALDNKEILACGLDVLANEPIDHTHPLMG
                     RDNVLITPHIGSASVTTRDNMIQLCINNIEAVMTNQVPHTPVN"
BASE COUNT         1000 a          391 c          506 g          856 t
ORIGIN      
        1 aatgaatgac ttcatttaaa tactcagtaa tttcatcgcc ttcttcagca tttacaccta
       61 aaatatgagc gatatagcct tcttctttta aatcatcagt accgataata ccgaatttat
      121 ttgtttgcat attaagtacg agtgtcttac cataatgtct atttgtatgg actaacatca
      181 aatcatatcg actatgctcg ccaacaaaac caacaaactg aacttgactc tcttcgttgt
      241 catcatataa atacatatca atcattttgt agcgactcct tttaaaagta gtaaagttag
      301 tataacgaca aatgaagtat actgcaaaat tatgataata tataagcgag aggtgacaag
      361 gaatgtattt tgtagacaaa gataaactaa ctcagaaatt agcctattta caagcattaa
      421 ctgatgatta tcatgagagc aagcacaatc attatgcatt tgaacgcatt gctcaaatgt
      481 tgatagaatc atcggtagat atagggaata tgattatcga tgcatttatt ttaagggatc
      541 ctggtaatta taaagatgtg attgatatat tagaactaga aaatgttatt actaaagaaa
      601 cacagcagga gattaataaa actgtcgata ttcgtaaaca atttacatat gattacacag
      661 ccttagatat taaggttatc atgccaatgt ttgatgacgc attaccttat tacaaacaat
      721 ttattacaga agtaacgata tttttacatc aagaaaatgt accagtaaca gcttttggta
      781 aaggagaaaa tcaataatga aacagtataa agcgtattta atcgatttag atggcacaat
      841 gtatatggga acagatgaga ttgatggagc aaaacaattc atcgattatt taaatgtaaa
      901 aggcattcct catttatacg taactaataa ttcaacaaaa acacctgagc aagtaactga
      961 aaaattacgt gaaatgcaca ttgatgctaa accagaagag gttgtaacgt cagcgttagc
     1021 cactgctgat tatatttcag aacaatcacc aggagcatca gtatatatgt taggtgggag
     1081 tggtttaaat actgcgttaa ccgaagcggg acttgtcatt aaaaatgacg agcatgttga
     1141 ttatgtagtt attggacttg acgaacaagt tacatatgaa aagcttgcga ttgcaacgtt
     1201 aggtgtaaga aatggtgcaa catttatttc tacaaatcct gatgtatcaa ttcctaaaga
     1261 gcgtggttta ttacctggta atggtgctat tacaagtgtt gtaagtgtat cgacaggtgt
     1321 atcgccacaa tttattggta aaccagaacc gattattatg gttaaagcat tagaaatttt
     1381 aggattagat aaatccgaag ttgctatggt aggcgatttg tacgataccg atattatgtc
     1441 tggtattaac gtaggtatgg atacgattca tgtacaaaca ggtgtatcta cgttagaaga
     1501 tgtgcaaaat aaaaatgtgc caccaacgta ttcttttaaa gatttaaatg aagcaatagc
     1561 tgaattagaa aaatagatat agtcatttta taaagtaggt gaattgattt ggtaaaaata
     1621 gttgtttcga ggaaaattcc agataaattt tatcaacaat taagtaaact tggtgacgtt
     1681 gttatgtggc aaaaatcatt agtgcctatg cctaaagatc aatttgtgac agcacttcgt
     1741 gacgcagatg cttgttttat tacattaagt gaacagatcg atgcagaaat tttagcacaa
     1801 tcaccaaatt taaaagtaat tgcgaatatg gctgtaggat atgacaacat cgatgttgaa
     1861 agtgcaacag cgaataacgt ggttgtcacg aatacaccaa atgtacttac tgaaacaact
     1921 gcagaattag gatttacatt aatgcttgct atagcacgcc gtattgtaga agctgaaaaa
     1981 tatgtagaag cagatgcatg gcaaagctgg ggtccttatt tattgtcagg taaagatgtc
     2041 ttcaattcaa ctattggaat atatggtatg ggagatattg gtaaagcttt tgcaagaagg
     2101 ttgcaagggt ttaatactaa tattctttat cataatcgat caagacataa agatgcagag
     2161 gcggacttta atgcaacata tgtttctttt gaaacgttgt tagcagaaag tgattttatc
     2221 atctgtacag cgccacttac aaaagaaaca catcataaat ttaatgctga agcatttgaa
     2281 caaatgaaaa atgatgcaat ttttattaat atcggtagag ggcaaattgt agatgaaaca
     2341 gcattaatcg atgcactaga caataaagaa attttagcat gtggtttaga tgtattagca
     2401 aatgaaccga ttgatcatac acatccatta atgggacgtg ataatgttct gattacacca
     2461 cacattggta gcgcatcagt aacaacacgg gacaatatga ttcaattatg tattaataat
     2521 atagaagcgg ttatgacaaa tcaggtacca catactccag taaattgaaa ataagtatgg
     2581 tttttaatca ttggtaaaac aagcaaagca attgttaaat atgttatgct tgacttgcga
     2641 atgtatatat ttgcgctgat gataattcaa taacattata aactcgtcat attaatgtga
     2701 tgagtttatt tgattttgac gtatataaca aaattgttat atagactgta att
//
LOCUS       DAHHSC010000251         2734 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.255, whole genome shotgun sequence.
ACCESSION   DAHHSC010000251 DAHHSC010000000
VERSION     DAHHSC010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2734)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2734)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2734
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.255"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            53..760
                     /gene="splA"
                     /locus_tag="NBI57_002474"
     CDS             53..760
                     /gene="splA"
                     /locus_tag="NBI57_002474"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease SplA"
                     /protein_id="HCD1823396.1"
                     /translation="MNKNVMVKGLTALTILTSLGFAENISNQPHSIAKAEKNVKEITD
                     ATKAPYNSVVAFAGGTGVVVGKNTIVTNKHIAKSNDIFKNRVAAHYSSKGKGGGNYDV
                     KDIVEYPGKEDLAIVHVHETSTEGLNFNKNVSYTKFAEGAKAKDRISVIGYPKGAQTK
                     YKMFESTGTINHISGTFIEFDAYAQPGNSGSPVLNSKHELIGILYAGSGKDESEKNFG
                     VYFTPQLKEFIQNNIEK"
     gene            885..1607
                     /gene="splB"
                     /locus_tag="NBI57_002475"
     CDS             885..1607
                     /gene="splB"
                     /locus_tag="NBI57_002475"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease SplB"
                     /protein_id="HCD1823397.1"
                     /translation="MNKNVVIKSLATLTILTSVTGIGTTLVEEVQQTAKAENNVTKIQ
                     DTNIFPYTGVVAFKSATGFVVGKNTILTNKHVSKNYKVGDRITAHPNSDKGNGGIYSI
                     KKIINYPGKEDVSVIQVEERAIERGPKGFNFNDNVTPFKYAAGAKAGERIKVIGYPHP
                     YKNKYVLYESTGPVMSVEGSSIVYSAHTESGNSGSPVLNSNNELVGIHFASDVKNDDN
                     RNAYGVYFTPEIKKFIAENIDK"
     gene            1665..2384
                     /gene="splC"
                     /locus_tag="NBI57_002476"
     CDS             1665..2384
                     /gene="splC"
                     /locus_tag="NBI57_002476"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease SplC"
                     /protein_id="HCD1823398.1"
                     /translation="MNKNIVIKSMAALAILTSVTGINAAVVEETQQIANAEKNVTQVK
                     DTNNFPYNGVVSFKDATGFVIGKNTIITNKHVSKDYKVGDRITAHPNGDKGNGGIYKI
                     KSISDYPGDEDISVMNIEEQAVERGPKGFNFNENVQAFNFAKDAKVDDKIKVIGYPLP
                     AQNSFKQFESTGTIKRIKDNILNFDAYIEPGNSGSPVLNSNNEVIGVVYGGIGKIGSE
                     YNGAVYFTPQIKDFIQKHIEQ"
     gene            2505..>2734
                     /gene="splD"
                     /locus_tag="NBI57_002477"
     CDS             2505..>2734
                     /gene="splD"
                     /locus_tag="NBI57_002477"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease SplD"
                     /protein_id="HCD1823399.1"
                     /translation="MNKNIIIKSIAALTILTSITGVGTTVVDGIQQTAKAENSVKLIT
                     NTNVAPYSGVTWMGAGTGFVVGNHTIITNKHVT"
BASE COUNT         1076 a          381 c          503 g          774 t
ORIGIN      
        1 atgtgtatat agatacatta actataaaaa taaatttgga aggaggtaaa atatgaataa
       61 aaatgtaatg gttaaaggtt taactgcttt aactatttta acatctcttg gtttcgctga
      121 aaatatttct aatcagcctc attcaattgc caaagcagaa aagaatgtca aagaaattac
      181 cgatgcaact aaggcaccat acaattcagt ggtagcattt gcgggtggta ctggtgtagt
      241 tgttggtaaa aatacaatcg taactaacaa acatatcgct aaaagtaatg atatttttaa
      301 aaatagagta gcagcacatt attcgagtaa aggtaaaggc ggaggaaact acgacgttaa
      361 agacattgta gaatatcctg gaaaagaaga ccttgcgata gttcatgttc atgaaacaag
      421 tacagaaggt ttgaatttta ataagaacgt tagttataca aaatttgcag aaggagcaaa
      481 agcgaaagat agaatttctg ttattggtta tccaaagggt gcacaaacaa aatataaaat
      541 gtttgaatcg acaggaacga ttaaccatat cagtggaacg tttatagaat ttgatgcgta
      601 tgcacaacca ggtaattcag gatctcctgt attgaattct aaacatgaac tgattggtat
      661 tttatatgca ggtagtggaa aagatgaatc tgaaaagaat ttcggtgttt atttcacacc
      721 acaattaaaa gaatttattc aaaataatat tgaaaaataa gtatcatcaa ttcattcgtg
      781 aagttgattt tttaaagagt gattaagaaa acggttacat aaccaactaa atatactgaa
      841 ttatgtatct agatatcaaa ataattaaaa aagaggaact taaaatgaac aaaaacgtag
      901 tcatcaagag tttagcaact ttaacaattt taacatctgt aacaggtatt ggaacaacat
      961 tggttgagga agttcaacaa actgccaaag cagaaaataa tgtcacaaaa attcaagata
     1021 ctaatatttt tccatatact ggtgtagttg cttttaaaag tgcaactgga tttgtagttg
     1081 gaaagaatac tattttaaca aataaacatg tgtcgaaaaa ttacaaagtg ggcgatcgta
     1141 ttactgcaca tccaaatagt gataaaggta atggtggtat ttattcgatt aaaaagatta
     1201 ttaattatcc aggtaaagaa gatgtatcag tcattcaagt tgaagagcgt gcaatagaac
     1261 gtggaccaaa aggctttaat tttaatgata atgtaacgcc attcaaatat gcggcagggg
     1321 ctaaagctgg tgagcgaatt aaagtgattg gttatccaca cccatacaaa aataaatatg
     1381 ttttatatga gtcaactggc cctgtgatgt cagtagaagg tagcagtatt gtatattcag
     1441 cgcatactga aagcggaaac tctggatcac ctgtattaaa cagcaacaac gaattagttg
     1501 gtattcattt tgcttctgat gtaaaaaatg atgataacag aaatgcatat ggcgtctact
     1561 ttacaccaga aattaaaaaa ttcattgcag aaaacataga taaataaaca aattgacttt
     1621 aaacgagcgt tgcaacatat ctcgaattgt aaaggagctt gaaaatgaat aaaaatatag
     1681 tcattaaaag catggcagca ttagccattc taacctcagt aactggaata aatgctgcag
     1741 tcgttgaaga gacacaacaa atagcaaatg cagagaagaa tgttacgcaa gttaaagata
     1801 caaataattt tccatataat ggcgtcgttt catttaagga tgcgacaggt tttgtaattg
     1861 gaaaaaatac aattatcacc aataaacatg tatcaaaaga ttataaagtt ggcgatagaa
     1921 ttactgccca tccaaacggt gacaaaggaa atggtggtat atataaaatt aaaagcattt
     1981 ctgattatcc gggtgatgaa gacatctctg tcatgaatat tgaagaacaa gcagttgaac
     2041 gtggaccaaa aggctttaat tttaatgaaa atgtccaagc attcaatttt gcgaaagatg
     2101 ctaaagttga tgacaaaatt aaagttattg gttacccatt acctgctcaa aatagtttta
     2161 aacagtttga atctacagga actataaaaa gaatcaaaga caatatttta aattttgatg
     2221 catacattga acccgggaat tcaggatcac cagttctaaa ttctaacaat gaggtcatag
     2281 gtgtggtgta tggcggtatt ggaaaaattg gttctgaata taatggtgcc gtatacttta
     2341 cgcctcaaat caaagatttt attcaaaagc acattgaaca ataaacaaat ttaaatatac
     2401 accatgagca tgtgttcaat aattttaatg aaaaacatcg gtcgaatata acataaaaaa
     2461 acgtctatat caaaagcatc atgaataaac agaggagcac aaaaatgaat aaaaatataa
     2521 tcatcaaaag tattgcggca ttgacgattt taacatcaat aactggtgtc ggcacaacag
     2581 tggttgatgg tattcaacaa acagccaaag cagaaaatag tgtgaaatta attaccaaca
     2641 cgaatgttgc accatacagt ggtgttacat ggatgggcgc tggaacagga tttgtagttg
     2701 ggaatcatac aatcattacc aataaacatg ttac
//
LOCUS       DAHHSC010000252         2709 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.256, whole genome shotgun sequence.
ACCESSION   DAHHSC010000252 DAHHSC010000000
VERSION     DAHHSC010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2709)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2709)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2709
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.256"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            214..717
                     /locus_tag="NBI57_002478"
     CDS             214..717
                     /locus_tag="NBI57_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000746375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCD1823400.1"
                     /translation="MKKRKKRKELDYKTRLAIAKYLNDGLNIIQISKILGRDDAVLYR
                     EVNKRSVDGVYNPLLAEEDAIKSRSISDEQVAKRRRLNDETKAYIEEKLSLKWSPRQI
                     ASQIEKDTGHYISYPTIYRYIRNGLVKVDVKRDMRQAGKKYNKSSEKRGKLDVGARVI
                     KYRLKKY"
     gene            911..1234
                     /locus_tag="NBI57_002479"
     CDS             911..1234
                     /locus_tag="NBI57_002479"
                     /inference="COORDINATES: protein motif:HMM:NF033563.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HCD1823401.1"
                     /translation="MDRGKEFALFEQIEDELNCSIYFSDLGCPYQRGSIENVNGLLRQ
                     YYPKGTDFANITQKSLDEAVKQINTRPRMIFDYKSSEEMLKYHVSTQNCEPILNDCVR
                     HEPVN"
     gene            complement(1456..1719)
                     /locus_tag="NBI57_002480"
                     /pseudo
     CDS             complement(1456..1719)
                     /locus_tag="NBI57_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499489.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA"
     gene            complement(1840..2559)
                     /gene="mecA"
                     /locus_tag="NBI57_002481"
     CDS             complement(1840..2559)
                     /gene="mecA"
                     /locus_tag="NBI57_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HCD1823402.1"
                     /translation="MRIERVDDTTVKLFITYSDIEARGFSREDLWTNRKRGEEFFWSM
                     MDEINEEEDFVVEGPLWIQVHAFEKGVEVTISKSKNEDMMNMSDDDATDQFDEQVQEL
                     LAQTLEGEDQLEELFEQRTKEKEAQGSKRQKSSARKNTRTIIVKFNDLEDVINYAYHS
                     NPITTEFEDLLYMVDGTYYYAVHFDSHVDQEVINDSYSQLLEFAYPTDRTEVYLNDYA
                     KIIMSHNVTAQVRRYFPETTE"
BASE COUNT          958 a          409 c          408 g          934 t
ORIGIN      
        1 aagaatgaga caacagataa atatgtaaaa gttgaagata tgaatgaaaa agaatatcaa
       61 agtaatcata aaccagttta tgaagaaaat aatatgaaaa aataaagaat caactcccta
      121 tacgctttag cggtggtatc gggagttgat gaacatatat cctcttataa cttattttat
      181 aaataagctt tttaaaagta aaggaggata tacatgaaaa aaagaaaaaa acgtaaagaa
      241 ttagattata aaacacgtct agctatcgca aagtatttaa atgatggttt aaacatcatt
      301 cagatttcta agattttagg tcgtgatgat gcggttcttt atagagaagt aaataagcgt
      361 tctgttgatg gtgtttataa tcctttatta gctgaggaag atgctataaa atcgcgtagt
      421 atttcagatg aacaagttgc taaacgtaga cgtttgaatg atgaaacaaa agcatatatt
      481 gaagaaaaac tttcattaaa atggtctcct agacagatag caagtcaaat tgaaaaagat
      541 acagggcatt atataagtta tcctactata tatagatata ttcgcaatgg attggtaaaa
      601 gttgatgtta agcgtgatat gagacaagca ggcaaaaaat ataataagtc ttctgaaaag
      661 cgtggcaaat tagacgtagg ggctcgtgtt ataaagtatc ggctcaaaaa gtattaataa
      721 ccgtgaagaa tttggacatt atgaaattga tactgtttgg agtgttagac catcaacata
      781 ttgcttgtta actattattg aaagaaaaac aagatattta tatgctagta gattaaatac
      841 acgtaaatcc aatgtagttt gtaattagat tataaatatt atgaaaccat tattaccaaa
      901 aagcataact atggatagag gtaaggagtt cgctctattt gaacaaatag aagatgaatt
      961 gaattgtagt atttatttta gtgatttggg ttgtccatat caacgtggtt ccattgaaaa
     1021 tgtaaatgga cttttaagac agtattatcc taaaggtacg gattttgcaa atataacaca
     1081 aaagtcatta gatgaagctg ttaaacaaat taatacacgg cctcgaatga tatttgacta
     1141 taaatcgagt gaggaaatgt taaagtacca tgtaagcaca caaaattgtg aacctatatt
     1201 aaatgactgt gtgaggcacg aacccgtcaa ttaatatagg ttaatctaag cgaatggttt
     1261 acaatgagcg cggaaggaca ggacgaataa attttgtata taaaatgcat aatctaaaaa
     1321 gaagcatact atttaaatct gtgatttttg acagatatta ctagtaaaat gcttcttttt
     1381 cttcttctgt actcttttag taatatttat taaaaaaaat taagaaattc tcatttttct
     1441 attgacacac tgcctttctt ttaaaaatgg ctctatctct actttacatc cttgttgctg
     1501 tatcatctgt gctaaaatca atttactttt ataatgttta atcgtttcat tattaaaaca
     1561 tttttgttcc gctaaatgtt tatgtgcaaa atgactaatt actttgagcc cacgctttaa
     1621 aataacttgc ttgccacaca caggacaaaa atattgtgtc tttctcaatg cagtagttgc
     1681 taacacacgt tccttttctt catttaaagc tactaacata tcttcacctc aaaatattac
     1741 acgtaagttt aactactatt actttttcag cttttgaaat caataaaaaa agagactcca
     1801 aagagtctcc tttcatagtg cgttgcttaa ttaatatcgt tattcagttg tctctggaaa
     1861 ataacgtcga acttgagctg ttacgttatg actcataatt attttagcat agtcatttaa
     1921 ataaacttct gttctgtctg ttggatatgc aaattcaagc aattgactgt aactatcatt
     1981 aatgacttct tgatcaacat gactatcaaa atgtacagca taataataag taccatcaac
     2041 catatataac aaatcttcaa actctgtagt tattggattg ctatgatatg cataattaat
     2101 aacatcttct aaatcgttaa atttcacaat gattgttctt gtatttttac gtgctgaaga
     2161 cttttgacgt ttagaacctt gagcttcttt ttcttttgtt cgttgctcga ataattcttc
     2221 taattgatct tcaccttcta atgtttgagc taacaattct tgaacttgtt catcaaattg
     2281 atcagttgca tcatcatcag acatattcat catatcttca tttttagatt tagaaattgt
     2341 gacttcgaca cctttttcaa aggcatgtac ttgaatccat aatggacctt ctacaacaaa
     2401 atcttcttct tcgttaattt catccatcat tgaccaaaag aattcttcgc cacgtttgcg
     2461 atttgtccat aaatcttcac gactaaatcc acgggcctcg atatcgctat atgttataaa
     2521 caattttaca gttgtatcat ctactcgttc tattctcata tcatctcact ccttacagtc
     2581 gatgaatagt atccacattg tattaaagca tcaaacaaaa tacaatttat ttgtttgatt
     2641 tctttctcta aaatattttg gtaacttata ataacatatt cgttgtagca aaactattta
     2701 aatgattta
//
LOCUS       DAHHSC010000253         2703 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.257, whole genome shotgun sequence.
ACCESSION   DAHHSC010000253 DAHHSC010000000
VERSION     DAHHSC010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2703)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2703)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2703
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.257"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            <1..327
                     /locus_tag="NBI57_002482"
     CDS             <1..327
                     /locus_tag="NBI57_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01741 family protein"
                     /protein_id="HCD1823403.1"
                     /translation="YYTDIPKEYNISVQVFDDLWMDLYDLFEELRDLFKEEDLEPWTS
                     CEFDFTREGKLKVSFDYIDWINTEFDQLGRENYYMYKKFGVIPEMEYEMEEVKQIEQY
                     IKEQEE"
     gene            530..1213
                     /locus_tag="NBI57_002483"
     CDS             530..1213
                     /locus_tag="NBI57_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5079 family protein"
                     /protein_id="HCD1823404.1"
                     /translation="MTTKENIDTLRKPGAQALSLISLFLILFSCLTFFFGLDYERFPN
                     YLKITTIIELIIIVISLLQWIRFIDFEKESARKYKKIYARFLVIINVLTTITVVFALC
                     NLYYFAAVQNHYDLFNYWLMGTISIIISYLLLVIGGMFTLLKLPKVTKRWGGKTKTHF
                     GLLLTALSSFIYIEKIIEYILIPNVVESKFIIIVSMLVIAGAQFVAFQFIMQYSRFYI
                     FELNTEDDD"
     gene            1359..1970
                     /locus_tag="NBI57_002484"
     CDS             1359..1970
                     /locus_tag="NBI57_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5080 family protein"
                     /protein_id="HCD1823405.1"
                     /translation="MEFLLLIVVAGLYYIIYLTAVMYSEKIVVLPIIIYAILFVIIGI
                     TYIFIGDSYDQLTNFNVILYMGSLFYAWMAIRNLWNRPLLLKYKNITDSSSGIVNKSE
                     YNSVESLRINIEIAKYKGIISLIVAIVLTVLMTLKSTPQITAETRDLSISFFILSLFI
                     IIIFAVWDLFIRVRKGAFAFVVIRPILFSCWIFILNMILSRLL"
     gene            2105..2323
                     /locus_tag="NBI57_002485"
     CDS             2105..2323
                     /locus_tag="NBI57_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1823406.1"
                     /translation="MENQKQGNGLKIATWVFIVLTVVTPLFGIGSIVCSINYKKYDAE
                     KGSKLLQIAIIVTIIAFVLNLLAYLGLR"
     gene            2455..>2703
                     /locus_tag="NBI57_002486"
     CDS             2455..>2703
                     /locus_tag="NBI57_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000142138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01741 family protein"
                     /protein_id="HCD1823407.1"
                     /translation="MTFEEKLSQMYNEIANKISSMIPVEWEKVYTMAYIDDEGGEVFY
                     YYTEPGSNELYYYTSVLNKYDISESEFMDSAYELYKQFQ"
BASE COUNT          991 a          276 c          473 g          963 t
ORIGIN      
        1 tattacaccg atatacctaa ggagtataac atttctgtgc aagtatttga tgatttatgg
       61 atggatttat atgatttgtt tgaggagtta agagatttat ttaaagaaga agatttagaa
      121 ccatggacat catgtgaatt tgactttaca agagagggta aattgaaagt atcatttgat
      181 tatatagatt ggataaatac agagtttgat caattgggcc gtgaaaatta ttatatgtac
      241 aaaaaatttg gggttatacc agaaatggaa tatgaaatgg aggaggttaa acaaatcgag
      301 caatatatta aagagcaaga agaatagaca atcttaatgt gagactaaac aataaagctt
      361 tgtttagtct tttttggcgg ttaagtaaaa agcaatagat accgtaaagt tgatgctcgt
      421 caaataataa tataaagata gttttatgct ttgtatagat aaaaacctaa gtacttataa
      481 ctttaaatac gtaatatgct atttaaaaga taatgtatag gagacagata tgactactaa
      541 agaaaatatt gatactcttc gaaagccagg tgcacaagcc ttaagtttaa tatcattgtt
      601 tttgatactt ttttcatgtc taactttctt ttttggttta gattatgaaa ggtttccaaa
      661 ctatttaaag ataacgacaa ttatagaatt aataattatt gtaattagtt tacttcaatg
      721 gattaggttt atagatttcg aaaaagaaag tgcacggaaa tataaaaaaa tatatgctcg
      781 atttttagtt attataaatg tgttaactac tatcactgta gtatttgcac tgtgtaacct
      841 ttattatttt gcagctgtac aaaaccatta tgatttattc aattattggt taatgggtac
      901 gatttcaatc ataattagct atttattatt agtaattggc ggaatgttta cgttgttaaa
      961 attacctaaa gtaacaaaac gctggggtgg taaaactaaa acacatttcg gtttattatt
     1021 aactgcgttg agctcattta tatatattga aaaaattatc gaatatatat tgattcctaa
     1081 tgtcgtagaa tccaagttta taattattgt gagcatgttg gttatcgctg gagcacaatt
     1141 tgtggcattt caatttatta tgcaatacag tagattctat atttttgaat taaacacaga
     1201 agatgatgac taaatgattt tgtaaatact gtgaagtata cttaaattaa gaaaatcaac
     1261 ttcaacgtat atcaccatat tattttatac tcaatgatgt gaatggattt aagtggattg
     1321 atattaaagt aaaagttatg gaatattgag gtgcgaatat ggagttctta ttattaattg
     1381 tcgtagccgg actgtattat attatatatt taactgctgt gatgtattct gaaaaaatag
     1441 tagtattgcc tataatcatc tatgccattt tgtttgtaat aattggtatc acttatatct
     1501 ttataggcga cagctatgat caattaacaa atttcaatgt gattttgtat atggggagtt
     1561 tgttttatgc atggatggct attagaaatc tttggaacag accactatta ctaaaatata
     1621 agaacattac agatagttca agtggaatag ttaataaatc tgaatacaat tcagttgaaa
     1681 gcttacgtat aaatattgaa atagctaagt ataaagggat tatttctttg atagtagcta
     1741 tagtactaac ggtattaatg acattaaagt caacacctca aatcactgca gaaacacgtg
     1801 acttaagtat ctcatttttc atactcagct tatttatcat tattatattt gcagtttggg
     1861 atttgtttat tagagttaga aaaggagcgt ttgcttttgt tgtaataagg ccaatattat
     1921 tcagttgttg gatatttatt ttgaatatga ttttatcaag attattataa ttgtattaca
     1981 tataggtgtg ggtaaatttt atttatgaaa tgatgaaaaa gtatatacta ttaatatctt
     2041 ttttactata attataaata gttaaataaa ttgaaagaca aaataatgag taggagttaa
     2101 tgagatggaa aaccaaaaac aaggcaatgg cttaaaaatt gcaacatggg tatttattgt
     2161 attaacagta gttacaccgc tatttggtat tggaagtatt gtttgtagta ttaattataa
     2221 aaaatacgat gctgaaaaag gttcgaagtt attgcaaatt gcaattatcg taacaataat
     2281 tgcttttgtt ttaaatttat tagcatattt aggtttaaga taagtaaaac gaatttgaag
     2341 aagcattaag cgacatttgg gtgttgttta atgcttttta tttaacggaa ataagtgtag
     2401 agaataaatt aatagtttaa tcaagagata tttcgaacac ataggggcga taacatgact
     2461 ttcgaagaaa aattaagtca aatgtacaac gagattgcga ataagattag tagcatgata
     2521 ccagtagagt gggaaaaagt atatacaatg gcttatatag atgatgaagg tggagaagtg
     2581 ttctactatt acacagaacc tggaagcaat gaattatact actatactag tgtattaaat
     2641 aaatatgata tatcggaatc agaatttatg gactcagcgt atgagttgta taaacaattt
     2701 caa
//
LOCUS       DAHHSC010000254         2662 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.258, whole genome shotgun sequence.
ACCESSION   DAHHSC010000254 DAHHSC010000000
VERSION     DAHHSC010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2662)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2662)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2662
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.258"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            277..1578
                     /locus_tag="NBI57_002487"
     CDS             277..1578
                     /locus_tag="NBI57_002487"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HCD1823408.1"
                     /translation="MRMIDIIEKKRDGHTLTTEEINFFIDGYVKGDIPDYQASSLAMA
                     IYFQDMNDDERAALTMAMVNSGDMIDLSDIKGVKVDKHSTGGVGDTTTLVLAPLVAAV
                     DVPVAKMSGRGLGHTGGTIDKLEAIDGFHVEIDEATFVKLVNENKVAVVGQSGNLTPA
                     DKKLYALRDVTGTVNSIPLIASSIMSKKIAAGADAIVLDVKTGSGAFMKTLEDAEALA
                     HAMVRIGNNVGRNTMAIISDMNQPLGRAIGNALELQEAIDTLKGQGPKDLTELVLTLG
                     SQMVVLANKAETLEEARALLIEAINSGAALEKFKTFIKNQGGDETVIDHPERLPQAQY
                     QIEYKAKKSGYVTELVSNDIGVASMMLGAGRLTKEDDIDLAVGIVLNKKIGDKVEEGE
                     SLLTIHSNRQDVDDVVKKLDSSITIADHVVSPTLIHKIITE"
     gene            1592..1942
                     /locus_tag="NBI57_002488"
     CDS             1592..1942
                     /locus_tag="NBI57_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1823409.1"
                     /translation="MNILRYLLGIGFSVIGVLHFTRERQFRNIIPKCLPLRKTAVLVT
                     GIFEILFGLALLIKRPSQCLKNTINLFLLAVFPANIYMAVKKLPLGDKQLPTWQLYLR
                     LPMQFVLMGLIKKL"
     gene            complement(2094..2564)
                     /locus_tag="NBI57_002489"
     CDS             complement(2094..2564)
                     /locus_tag="NBI57_002489"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HCD1823410.1"
                     /translation="MTKMNVESFNLDHTKVVAPFIRLAGTMEGLNGDVIHKYDIRFKQ
                     PNKEHMDMPGLHSLEHLMAENIRNHSDKVVDLSPMGCQTGFYVSFINHDNYDDVLNIV
                     EATLNDVLNATEVPACNEVQCGWAASHSLEGAKTIAQAFLDKRNEWHDVFGTGK"
BASE COUNT          909 a          388 c          508 g          857 t
ORIGIN      
        1 ttataaaaat atgtaagcat gggacatcgt aaccatatat aagtatgtcg atatcagctt
       61 taagacatca taaatgccat gatgattcta agataattta atagggactt taacgttata
      121 accaatctat ttaagaataa taacatcgca ttgaaattgt gatattaaaa atgaaatcga
      181 tagcttgcta taataatttt cgcttgtgct tttaaaaatg gactagagaa agcgcagatg
      241 ctttgtccta agctttttaa gaaaaggatg ataaaaatga gaatgataga cattattgag
      301 aaaaagcgtg acggtcatac actaacaacg gaagaaatta atttctttat tgacggctat
      361 gttaaagggg atattcctga ttaccaagca tcaagtttag caatggcgat ttatttccaa
      421 gatatgaatg atgatgagcg tgcagcatta acgatggcta tggttaattc tggtgatatg
      481 atagatttga gtgatattaa aggtgtcaaa gtagataagc actcaacagg tggtgtagga
      541 gatacaacta cattggtttt agcaccatta gtagcagctg tagatgttcc tgttgcaaaa
      601 atgagtgggc gtggattagg tcatacaggt ggtacgattg ataaattaga agcaattgat
      661 ggttttcatg ttgaaataga tgaagcaaca tttgtgaaat tggttaatga aaataaagtg
      721 gcagttgtag gacaatcagg aaatttaact cctgcagaca aaaaattata tgccttaagg
      781 gatgttactg gtactgtcaa ttcaatacca ttaattgcct cttcaattat gagtaaaaag
      841 attgctgctg gtgcagatgc aattgtatta gatgtaaaaa ctggtagcgg tgcatttatg
      901 aaaacattag aagatgctga agcattagcg cacgcaatgg tacgtattgg taataatgtg
      961 ggacgtaata cgatggcgat tatttctgat atgaatcagc cacttggacg tgcgattggc
     1021 aatgcgcttg agttacaaga agcaattgat acgttgaaag gacaaggtcc gaaagattta
     1081 acagaacttg tattaacatt aggttctcaa atggttgtac ttgcaaacaa agctgaaacg
     1141 cttgaagaag cgagagcgtt gctaattgag gcaattaatt caggtgcagc attagaaaaa
     1201 ttcaaaacat ttattaaaaa ccaaggtggc gacgaaactg ttattgacca tccagagcgt
     1261 ttgccacaag ctcaatatca aattgaatat aaagctaaaa aatcaggtta tgtgactgaa
     1321 ttagtctcta acgatatagg tgtcgcttcg atgatgttag gagcgggacg tttaacaaaa
     1381 gaggatgata ttgatttagc ggttggtatt gttttaaata aaaaaattgg tgataaagta
     1441 gaagaaggag aatcattatt aacgattcac agtaatcgcc aagatgtaga cgatgttgtt
     1501 aaaaaattag attcaagcat aacaattgct gatcatgttg tatcacctac attaattcat
     1561 aaaatcatta cagagtagga gttgaagctg attgaacata ctaagatatt tattaggaat
     1621 aggttttagt gtaataggtg tattgcattt tacacgagaa cgacaattta gaaatatcat
     1681 accgaaatgt ttgccacttc gaaaaacagc ggttcttgtt acaggaatct ttgagatatt
     1741 atttggactt gcacttttaa ttaaaagacc atctcaatgc ttgaaaaata caatcaatct
     1801 attcttgcta gctgtattcc cagctaatat ctatatggca gttaaaaagt tacctttagg
     1861 agataaacaa ttgccaacat ggcaactata tttaaggtta cctatgcagt tcgtgttaat
     1921 gggactaatt aagaaattat agtggttctc aaataaatcg gacaaatgtt aaagatttta
     1981 ctttttcatt tgttcgattt tttgactttg aagcataata aaaagcagtt ggacaatggc
     2041 gattgtaaca tcattgttcg actgcttttc tgattaactt gattgactaa gatttatttt
     2101 cctgtaccga aaacatcatg ccattcgttt cgtttgtcta gaaatgcttg agcgatagtt
     2161 ttagcacctt ctaatgaatg acttgctgcc cagccacatt gtacttcatt acaagcaggc
     2221 acttcagtag catttagcac atcatttaaa gttgcttcaa caatatttaa tacatcatca
     2281 taattatcat gattaataaa tgatacatag aaaccagttt ggcaacccat aggacttaaa
     2341 tcaacaactt tgtcactatg atttctaata ttttcagcca ttaaatgttc taatgaatgt
     2401 agtcctggca tatccatatg ttctttgttt ggttgtttga aacgaatgtc gtatttgtga
     2461 atgacatctc cgtttaatcc ttccatcgtt cccgctaatc taataaatgg ggcaaccact
     2521 ttagtatgat ctaaattgaa actttcaaca ttcatttttg tcattttgaa tttcctccta
     2581 tttactactc aaaactataa aacgcttatg atttgttgca atataaaact taagtttgtt
     2641 gtaagttgtg tttctaacaa ta
//
LOCUS       DAHHSC010000255         2631 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.259, whole genome shotgun sequence.
ACCESSION   DAHHSC010000255 DAHHSC010000000
VERSION     DAHHSC010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2631)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2631)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2631
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.259"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            313..981
                     /gene="queC"
                     /locus_tag="NBI57_002490"
     CDS             313..981
                     /gene="queC"
                     /locus_tag="NBI57_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HCD1823411.1"
                     /translation="MESVLNNEKAIVVFSGGQDSTTCLFYAKKHFKEVELVTFNYGQR
                     HDTEIEVAKQIAQDQGMKHHVLDMSLLSQLTPNALTQHDMEITNNEDGIPNTFVPARN
                     LLFLSFAGALAYQIGAKHIITGVCETDFSGYPDCRDSFIKSMNVTLSLAMDKDFVIHT
                     PLMWLNKAETWKLSDELEVLDYIRTKTLTCYNGIIGDGCGECPACHLRQRGLNQYLES
                     KGAL"
     gene            983..1402
                     /gene="queD"
                     /locus_tag="NBI57_002491"
     CDS             983..1402
                     /gene="queD"
                     /locus_tag="NBI57_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HCD1823412.1"
                     /translation="MLQQIYPSTTHPYQFELNKDFNFSAAHHIPCEEAGICQNVHGHT
                     YFVNLTIVGDKLDDTGFLVNFSHLKKMIHGKFDHQLLNNLPAFKNKIPSTEIVAETIY
                     QIVKENLASLEHQPKCIQVFVRETPTSYVVFRPKEQV"
     gene            1406..2119
                     /gene="queE"
                     /locus_tag="NBI57_002492"
     CDS             1406..2119
                     /gene="queE"
                     /locus_tag="NBI57_002492"
                     /EC_number="4.3.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HCD1823413.1"
                     /translation="MAKIPVLEIFGPTIQGEGRVIGRKTMFVRTAGCDYRCSWCDSAF
                     TWDGSAKGDIKLMTAEEIYDELKRIGGDLFNHVTISGGNPALIKGIQELVDLFQDKGI
                     FSALETQGSKFQPWMTQIDDLTISPKPPSSTMTPDLKKLDEVITQCVPSSLNLKVVVF
                     DDKDYDFAKMIHHRYPDIPFYLQVGNPYLSDSVDNHTEKLLERYEQLVDLVMQSNDMN
                     HVYVLPQLHTLLWSNKKGV"
     gene            2218..>2631
                     /locus_tag="NBI57_002493"
     CDS             2218..>2631
                     /locus_tag="NBI57_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1823414.1"
                     /translation="MILYILINIAIVVLITGFNLYRHQMQHLSLSAMLLSITINAFIN
                     TFIIDKYNFITLCTITMFIIWTILQFYIDKKLKPVYITDQKFIAIILTIVVSLTQRVT
                     DFSSTQSIYMSIPFLAPAIFIIGGIMLFISTFNSLD"
BASE COUNT          922 a          418 c          426 g          865 t
ORIGIN      
        1 tgtattggat gtagctaaaa ctttaaaagc attgtcatta tcctgacctt gattttaact
       61 aatatatggt atattctatt catcgtacat aaatgaatat cagaggtttc tagctgaaac
      121 cctctataaa aaactagaca ttgaaatttc aaacattcgt tggtaaacct ttaatatata
      181 ttataggagc aacccatttc tttcgtctat cttttatgga gataggcttt ttttatttta
      241 taaattaaaa acgtgcttat caatgccatt aacctcttta ttaatattat taatgatagg
      301 agcaattaca aaatggaaag tgtattaaat aatgaaaaag ccatcgttgt ctttagtggt
      361 ggtcaagaca gtacaacatg tctcttttat gcaaaaaaac atttcaaaga agttgaactc
      421 gtaacgttta attatggcca aagacatgat actgaaattg aagtcgcaaa acaaattgca
      481 caagatcaag gaatgaaaca tcacgtttta gatatgtcat tattatcaca acttactcca
      541 aacgcattaa cacaacatga tatggaaatt actaataatg aagatggtat acctaataca
      601 tttgttccag ctagaaattt acttttcttg tcgtttgcag gcgctctagc ttatcaaatt
      661 ggggctaagc atattattac aggcgtatgt gaaacagact tttcaggcta cccagactgt
      721 cgcgatagtt ttataaaatc aatgaacgta acattaagcc tagctatgga caaagatttt
      781 gtcattcata ctcctttaat gtggttaaac aaagcagaaa cgtggaaatt aagtgatgaa
      841 ctcgaagttt tagattatat tcgtacaaaa acattaacat gctataacgg tatcattggg
      901 gatggctgtg gtgaatgtcc agcttgtcat ttacgtcaac gtggactaaa tcaatatctt
      961 gaaagtaaag gagcgcttta aaatgttaca acaaatctat cctagtacaa cgcatccata
     1021 tcaattcgaa ttaaataaag attttaattt ttcggctgca catcacattc cttgtgaaga
     1081 agcaggtatt tgtcaaaatg tccatggtca tacttacttt gttaatttaa caattgtcgg
     1141 tgataaacta gatgacactg gcttcttagt gaactttagc catttgaaaa agatgataca
     1201 cggtaaattt gaccatcaac tgttaaataa cttacctgct tttaaaaaca aaatcccttc
     1261 aactgaaatc gtagcggaaa caatttatca aattgttaaa gaaaatttgg catcgctcga
     1321 acaccaacca aaatgtattc aagtatttgt aagagaaaca ccaacaagtt atgttgtatt
     1381 tagaccaaag gaacaggtgt aactaatggc taaaattcct gtactcgaaa tattcggtcc
     1441 aaccattcag ggtgaaggtc gagtaatcgg tagaaaaacg atgtttgtga gaacagctgg
     1501 atgtgattat cgctgtagct ggtgtgattc tgcatttact tgggatggta gtgctaaagg
     1561 cgatataaaa ctcatgaccg ctgaagaaat ttatgatgaa ttaaaacgaa ttggtggcga
     1621 tttatttaat cacgttacaa tttctggtgg taacccagca ttaattaaag gtatccaaga
     1681 gttagttgac ttatttcaag ataaaggtat tttcagcgca ctggagacac aaggcagtaa
     1741 attccaacct tggatgacac aaattgatga tttaacgatt agtccaaaac caccaagttc
     1801 aactatgaca cctgatttaa aaaagttaga tgaagtgatt acacaatgcg taccttcctc
     1861 attaaactta aaagtagttg tattcgacga taaagattat gattttgcca aaatgataca
     1921 ccaccgttat ccagatattc ccttctattt acaagttggt aacccatatt tatcagacag
     1981 cgtagataat cataccgaaa agttgttaga acgctacgag cagttggtgg atttagttat
     2041 gcaaagtaat gatatgaatc acgtttatgt gttaccacaa cttcatacat tactttggag
     2101 taataaaaaa ggtgtataaa ggataactta ttttactttt tacaattcat atctaatgaa
     2161 taacaatata ttaattgata taatagttga ttgaagtata acgaacagga gtccatcatg
     2221 attttatata tattaattaa tattgctatc gtcgtactta taactggatt caatttatat
     2281 aggcatcaaa tgcaacattt atcattaagt gcaatgttgt tatctattac gataaacgcc
     2341 tttataaata cattcataat tgataaatat aactttatta ctctgtgtac tataacgatg
     2401 tttatcattt ggacgatact acagttttac attgataaaa aattaaaacc tgtatacatt
     2461 acagaccaaa aatttattgc tatcatatta acgattgttg tcagtttaac gcaacgtgtt
     2521 accgactttt caagtacaca atcaatatat atgtctattc catttttagc ccctgcaatt
     2581 ttcatcattg gtggtattat gttgtttatt tcaacattta atagcttaga t
//
LOCUS       DAHHSC010000256         2586 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.260, whole genome shotgun sequence.
ACCESSION   DAHHSC010000256 DAHHSC010000000
VERSION     DAHHSC010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2586)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2586)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2586
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.260"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            complement(94..654)
                     /gene="icaR"
                     /locus_tag="NBI57_002494"
     CDS             complement(94..654)
                     /gene="icaR"
                     /locus_tag="NBI57_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ica operon transcriptional regulator IcaR"
                     /protein_id="HCD1823415.1"
                     /translation="MKDKIIDNAITLFSEKGYDGTTLDDIAKSVNIKKASLYYHFDSK
                     KSIYEQSVKCCFDYLNNIIMMNQNKSNYSIDALYQFLFEFIFDIEERYIRMYVQLSNT
                     PEEFSGNIYGQIQDLNQSLSKEIAKFYDESKIKMTKEDFQNLILLFLESWYLKASFSQ
                     KFGAVEESKSQFKDEVYSLLNIFLKK"
     gene            818..2056
                     /gene="pgaC"
                     /locus_tag="NBI57_002495"
     CDS             818..2056
                     /gene="pgaC"
                     /locus_tag="NBI57_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6 N-acetyl-D-glucosamine synthase"
                     /protein_id="HCD1823416.1"
                     /translation="MQFFNFLLFYPVFMSIYWIVGSIYFYFTREIRYSLNKKPDINVD
                     ELEGITFLLACYNESETIEDTLSNVLALKYEKKEIIIINDGSSDNTAELIYKIKENND
                     FIFVDLQENRGKANALNQGIKQASYDYVMCLDADTIVDQDAPYYMIENFKHDPKLGAV
                     TGNPRIRNKSSILGKIQTIEYASLIGCIKRSQTLAGAVNTISGVFTLFKKSAVVDVGY
                     WDTDMITEDIAVSWKLHLRGYRIKYEPLAMCWMLVPETLGGLWKQRVRWAQGGHEVLL
                     RDFFSTMKTKRFPLYILMFEQIISILWVYIVLLYLGYLFITANFLDYTFMTYSFSIFL
                     LSSFTMTFINVIQFTVALFIDSRYEKKNMAGLIFVSWYPTVYWIINAAVVLVAFPKAL
                     KRKKGGYATWSSPDRGNTQR"
     gene            2020..2325
                     /gene="icaD"
                     /locus_tag="NBI57_002496"
     CDS             2020..2325
                     /gene="icaD"
                     /locus_tag="NBI57_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intracellular adhesion protein D"
                     /protein_id="HCD1823417.1"
                     /translation="MVKPRQREYPTLKSSLNIVRETALIAISCVFWIYCLVVLLVYIG
                     TIFEIHDESINTIRVALNIENTEILDIFETMGIFAIIIFVFFTISILIQKWQRGRES"
     gene            2322..>2586
                     /locus_tag="NBI57_002497"
     CDS             2322..>2586
                     /locus_tag="NBI57_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intercellular adhesin biosynthesis
                     polysaccharide N-deacetylase"
                     /protein_id="HCD1823418.1"
                     /translation="MKYRKLIILVLSILIILPVSTLDGHHIANADDDSPKKLKYKENS
                     ALALNYHRVRKANFLNNFIYFFSSSKEIKNYSVSQSQFESQIKW"
BASE COUNT          887 a          387 c          404 g          908 t
ORIGIN      
        1 gatttgtatt gtcaacttta tatagattta aaaaaataat ctcatgtctt tttttacaaa
       61 aataagttaa ttattacaaa ctagtaacaa aaattatttc ttcaaaaata tatttagtag
      121 cgaatacact tcatctttga attgactttt actttcttcc actgctccaa atttttgcga
      181 aaaggatgct ttcaaatacc aactttcaag aaacagcaat attaaattct gaaagtcttc
      241 ttttgtcatc tttatctttg attcatcata gaattttgct atctctttac ttaatgattg
      301 atttaaatct tgtatttgtc cgtaaatatt tccagaaaat tcctcaggcg tattagataa
      361 ttgaacgtac attctaatat acctttcttc gatgtcgaaa ataaactcaa ataagaattg
      421 atataaagca tcaattgaat agttcgattt attttgattc atcataataa tattattaag
      481 gtaatcaaaa cagcatttaa cactttgttc gtaaatactt tttttcgagt caaaatggta
      541 atataaactc gctttcttta tatttacact tttagctata tcatcaagtg ttgtaccgtc
      601 ataccccttc tctgaaaata aggttattgc gttatcaata atcttatcct tcaattttta
      661 taacccccta ctgaaaatta atcacactat gttacaggaa aattaagttg caattacaaa
      721 tatttccgtt taattataac aacaatctat tgcaaattaa aatactatca attaccatat
      781 ggcttacaac ctaactaacg aaaggtaggt aaagaaattg caatttttta actttttgct
      841 tttttatcct gtatttatgt ctatttactg gattgtcggt tcaatttatt tctattttac
      901 cagagaaatt agatattcat tgaacaagaa gcctgacata aatgtggatg aattagaagg
      961 cattacattt ttacttgcct gttataacga aagtgaaacg attgaagata cgttgtctaa
     1021 tgttcttgca ctcaaatacg agaagaaaga aattattatc attaatgatg gaagttcaga
     1081 taatacagca gaactcatct ataaaatcaa agaaaataat gactttattt tcgtcgattt
     1141 acaagaaaac agaggtaaag ccaacgcact caatcaaggc attaaacagg cttcatatga
     1201 ttatgtaatg tgcttggatg cagatactat cgttgatcaa gatgcaccat attatatgat
     1261 tgagaatttc aaacatgatc caaaacttgg tgcagttaca ggtaatccta gaattcgaaa
     1321 taagagttct attttaggta aaattcaaac gatagaatat gcaagtttaa ttggctgtat
     1381 taagcgaagt cagacacttg ctggcgcagt caatactatt tcgggtgtct tcactctatt
     1441 taaaaaaagt gcagttgtcg acgttggcta ctgggatact gatatgatta ccgaagatat
     1501 tgcagtttct tggaaattgc atttacgtgg atatcgtatt aagtatgaac cgcttgccat
     1561 gtgttggatg ttggttccag aaacattggg aggtctttgg aagcaacgcg tgagatgggc
     1621 tcaaggggga cacgaagtat tactacgaga cttttttagc acaatgaaaa cgaaaaggtt
     1681 tcctttatat attttgatgt ttgagcaaat catctcaatt ttatgggtat atatagtgct
     1741 tctatattta ggctatttgt tcataacagc aaacttctta gactatacat ttatgacata
     1801 tagtttttca atatttctac tatcatcatt tactatgact tttataaacg ttattcaatt
     1861 tacagtcgca ctctttattg atagtcgcta cgagaaaaag aatatggctg gactcatatt
     1921 tgtaagttgg tatccgacag tatactggat tattaacgca gcagtagttc ttgtcgcatt
     1981 tccaaaagca ttaaaacgta agaaaggtgg ttacgcaaca tggtcaagcc cagacagagg
     2041 gaatacccaa cgctaaaatc atcgctaaat attgtaagag aaacagcact tatcgctata
     2101 tcgtgtgtct tttggatata ttgtttagtt gttctactcg tttatattgg tactatattt
     2161 gaaattcatg acgaaagtat caatacaata cgtgttgctt taaacattga aaatactgaa
     2221 attttagata tatttgaaac tatgggcatt ttcgcgatta tcatttttgt attttttaca
     2281 attagcatat tgattcaaaa atggcagaga ggaagagaat cgtgaagtat agaaaattaa
     2341 taattttagt gttgagtatc ttgatcatat tgcctgtaag cacactggat ggtcatcata
     2401 ttgcaaatgc agatgacgat tcacctaaaa aactgaaata taaagaaaat agtgctctgg
     2461 cattaaatta tcaccgtgta agaaaagcga attttctgaa taattttatt tacttctttt
     2521 ctagtagtaa agaaattaaa aattatagtg ttagtcaatc acaatttgaa tctcaaataa
     2581 aatggc
//
LOCUS       DAHHSC010000257         2580 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.261, whole genome shotgun sequence.
ACCESSION   DAHHSC010000257 DAHHSC010000000
VERSION     DAHHSC010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2580)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2580)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2580
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.261"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            101..385
                     /gene="groES"
                     /locus_tag="NBI57_002498"
     CDS             101..385
                     /gene="groES"
                     /locus_tag="NBI57_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HCD1823419.1"
                     /translation="MLKPIGNRVIIEKKEQEQTTKSGIVLTDSAKEKSNEGVIVAVGT
                     GRLLNDGTRVTPEVKEGDRVVFQQYAGTEVKRDNETYLVLNEEDILAVIE"
     gene            461..2077
                     /gene="groL"
                     /locus_tag="NBI57_002499"
     CDS             461..2077
                     /gene="groL"
                     /locus_tag="NBI57_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500736.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HCD1823420.1"
                     /translation="MVKQLKFSEDARQAMLRGVDQLANAVKVTIGPKGRNVVLDKEFT
                     APLITNDGVTIAKEIELEDPYENMGAKLVQEVANKTNEIAGDGTTTATVLAQAMIQEG
                     LKNVTSGANPVGLRQGIDKAVKVAVEALHENSQKVENKNEIAQVGAISAADEEIGRYI
                     SEAMEKVGNDGVITIEESNGLNTELEVVEGMQFDRGYQSPYMVTDSDKMVAELERPYI
                     LVTDKKISSFQDILPLLEQVVQSNRPILIVADEVEGDALTNIVLNRMRGTFTAVAVKA
                     PGFGDRRKAMLEDLAILTGAQVITDDLGLDLKDASIDMLGTASKVEVTKDNTTVVDGD
                     GDENSIDARVSQLKSQIEETESDFDREKLQERLAKLAGGVAVIKVGAASETELKERKL
                     RIEDALNSTRAAVEEGIVAGGGTALVNVYQKVSEIEAEGDIETGVNIVLKALTAPVRQ
                     IAENAGLEGSVIVERLKNAEPGVGFNAATNEWVNMLEAGIVDPTKVTRSALQHAASVA
                     AMFLTTEAVVASIPEKNNDQPNMGGMPGMM"
BASE COUNT          935 a          342 c          548 g          755 t
ORIGIN      
        1 ttatgacttg aaattttgac caaatttgat tattataaat gtatgttagc actctttaat
       61 gttaagtgct aaactttagg ttttttaagg aggaacaatc atgctaaaac caattggaaa
      121 tcgtgtgatt attgagaaaa aagaacaaga acaaacaact aaaagtggta ttgttttaac
      181 tgatagtgct aaagaaaaat caaacgaagg cgttatcgtt gcagtaggaa ctggacgtct
      241 attaaatgat ggtacaagag tgactcctga agtgaaagaa ggggaccgtg tcgtgttcca
      301 acaatatgct ggtacagaag ttaaacgaga taatgaaaca tatctagtat taaatgaaga
      361 agatatttta gcggtaattg aataatataa aattaaattc atagataaat tgtaaagaac
      421 gaaaatgaaa tatgactaaa caaatggagg tttatcattt atggttaaac aattgaaatt
      481 ctctgaagat gcacgtcaag caatgttacg tggtgtagac caattagcaa atgcagttaa
      541 agtaacgatt ggtcctaaag gacgtaatgt tgtattagat aaagagttta cagcaccttt
      601 aattacgaac gatggtgtaa cgattgctaa agaaatcgaa ttagaagatc catatgaaaa
      661 tatgggggct aaactagttc aagaagttgc aaataagaca aatgaaattg ctggtgacgg
      721 tacgacaact gcaacagtat tagctcaagc aatgattcaa gaaggcttga aaaatgttac
      781 aagtggtgcg aacccagttg gtttacgtca aggtatcgac aaagcagtta aagttgctgt
      841 agaagcatta catgaaaatt ctcaaaaagt tgaaaataaa aatgaaatag cgcaagtagg
      901 tgcgatttca gcagcagatg aagaaattgg acgttatatt tctgaagcta tggaaaaagt
      961 aggtaacgat ggtgtcatta caattgaaga atcaaatgga ctaaacactg aactagaagt
     1021 ggttgaaggt atgcaatttg atcgtggtta tcaatcacca tatatggtta ctgattcaga
     1081 taaaatggtt gctgaattag aacgcccata cattttagta acagataaga aaatctcgtc
     1141 tttccaagat atcttacctt tattagaaca agtggttcaa tctaatcgtc caatcttaat
     1201 tgtagctgat gaagttgaag gcgatgcatt aacaaatatc gtgctaaacc gaatgcgtgg
     1261 cacatttaca gctgttgcag taaaggcgcc tggtttcggt gatcgtcgta aagcaatgtt
     1321 agaagattta gctattttaa ctggtgcgca agtgattact gatgatttag gcttagattt
     1381 aaaagatgca tcaattgata tgttaggtac tgcaagtaaa gtagaagtaa ctaaagataa
     1441 taccactgtt gttgatggtg acggtgacga aaacagcatt gatgcacgtg taagccaatt
     1501 gaaatctcaa attgaagaaa ctgaatctga ctttgatcgt gaaaaattac aagagcgctt
     1561 agctaaatta gcaggtggtg ttgcagttat taaagtaggt gcagcaagtg aaacagagct
     1621 taaagaacgt aaattacgta ttgaagatgc attaaattct acacgtgcag cagttgaaga
     1681 aggtattgtt gcaggtggtg gtactgcatt agtaaatgtt taccaaaaag taagtgaaat
     1741 tgaagcagaa ggtgacattg aaacaggtgt aaatattgta cttaaagcac taactgcacc
     1801 agttcgtcaa attgctgaaa atgcaggatt agaaggttct gttattgtag aacgtttgaa
     1861 aaacgcagag ccgggtgttg gttttaacgc tgctacaaac gagtgggtta atatgttaga
     1921 agcaggtatc gttgatccaa ctaaagtaac acgctcagca ttacaacatg ctgcaagtgt
     1981 tgcagcaatg ttcttaacga ctgaagcggt tgtagcatca attccagaaa aaaataatga
     2041 ccaacctaac atgggtggca tgccgggaat gatgtaaaac agtttctagt tttgactaac
     2101 tagaacattc catgatagac gagtatgatt cgttccaaat aaatattaga gcgtatgaaa
     2161 atattttaat ttaagacacc ttccattagt tgactaaact tatgagaggc gtctttttta
     2221 tgtgctaatt tgtaattgag attcataatg gtatttaaat cattatggtg cgggagttat
     2281 ggtattgcca taatatgaga gtgcatccac ttctataagt aatgcatatt gcgagtgcag
     2341 gaatgaatga gtgcttgatt aaaatcctta tgggtggttg acataattaa aagaaaccac
     2401 atttaaaatt tcttaatcac aagcggttaa ctaggtatag tttagttttg agtaaatctt
     2461 tttaaaggtg tacttgtgca ttttacttaa ttaaagagat aagacattta atgggcctaa
     2521 aataaataaa aaacaaaaaa ctacctgttt aggtagtttt ttaaatgtaa tagattaaaa
//
LOCUS       DAHHSC010000258         2575 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.262, whole genome shotgun sequence.
ACCESSION   DAHHSC010000258 DAHHSC010000000
VERSION     DAHHSC010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2575)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2575)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2575
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.262"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            complement(346..1329)
                     /gene="lukD"
                     /locus_tag="NBI57_002500"
     CDS             complement(346..1329)
                     /gene="lukD"
                     /locus_tag="NBI57_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bi-component leukocidin LukED subunit D"
                     /protein_id="HCD1823421.1"
                     /translation="MKMKKLVKSSVASSIALLLLSNTVDAAQHITPVSEKKVDDKITL
                     YKTTATSDNDKLNISQILTFNFIKDKSYDKDTLVLKAAGNINSGYKKPNPKDYNYSQF
                     YWGGKYNVSVSSESNDAVNVVDYAPKNQNEEFQVQQTLGYSYGGDINISNGLSGGLNG
                     SKSFSETINYKQESYRTTIDRKTNHKSIGWGVEAHKIMNNGWGPYGRDSYDPTYGNEL
                     FLGGRQSSSNAGQNFLPTHQMPLLARGNFNPEFISVLSHKQNDTKKSKIKVTYQREMD
                     RYTNQWNRLHWIGNNYKNQNTVTFTSTYEVDWQNHTVKLIGTDSKETNPGV"
     gene            complement(1331..2266)
                     /gene="lukE"
                     /locus_tag="NBI57_002501"
     CDS             complement(1331..2266)
                     /gene="lukE"
                     /locus_tag="NBI57_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_114304764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bi-component leukocidin LukED subunit E"
                     /protein_id="HCD1823422.1"
                     /translation="MFKKKMLAATLSVGLIAPLASPIQESRANTNIENIGDGAEVIKR
                     TEDVSSKKWGVTQNVQFDFVKDKKYNKDALIVKMQGFINSRTSFSDVKGSGYELTKRM
                     IWPFQYNIGLTTKDPNVSLINYLPKNKIETTDVGQTLGYNIGGNFQSAPSIGGNGSFN
                     YSKTISYTQKSYVSEVDKQNSKSVKWGVKANEFVTPDGKKSAHDRYLFVQSPNGPTGS
                     AREYFAPDNQLPPLVQSGFNPSFITTLSHEKGSSDTSEFEISYGRNLDITYATLFPRT
                     GIYAERKHNAFVNRNFVVRYEVNWKTHEIKVKGHN"
BASE COUNT          805 a          435 c          340 g          995 t
ORIGIN      
        1 cgataatagt ttctatttca attaaatcac cgatacttaa aatgataaat ttaccaatac
       61 caagtttgta atgaactttc accgtcatac aatcatagat gctatatcta caaaacgaac
      121 cacgccgatt aagacgtggt tccatttttt taataatttt ttatatgttg gaagtttcta
      181 aataattcta ctttcgtaaa tttagcaccc tgctatttag caaagtaatc tacatttccc
      241 taactaaaaa ttatcatgtg tgtaaataaa aattatgtta gttctttaat tccgtttgat
      301 aattgaaata attgcacttt gttgattatt taatcatata caattctata ctccaggatt
      361 agtttcttta gaatccgtac cgattaattt aacagtatgg ttttgccagt caacttcata
      421 agtagatgta aacgttactg tattttgatt tttgtagtta ttaccaatcc agtgtagtcg
      481 attccattga ttagtatatc tatccatttc tctttggtaa gttactttga ttttagattt
      541 ttttgtatca ttttgtttat gagaaagtac gcttataaat tctgggttaa agttaccacg
      601 cgccaataaa ggcatttgat gtgttggcaa gaaattttgt ccagcatttg aactactttg
      661 tctaccacct aaaaacagtt cattaccata tgttgggtca taactatctc taccatatgg
      721 tccccaacca ttattcataa ttttgtgcgc ctcaacaccc cagccaattg atttatgatt
      781 tgtttttcta tcaatcgtag ttctgtaact ttcttgttta taatttattg tttctgaaaa
      841 tgattttgat ccattcaatc cacctgataa gccattagat atattaatat ctccgccata
      901 agaataacct aatgtttgtt gaacttggaa ttcttcattt tgatttttag gtgcatagtc
      961 aacaacattt acagcatcat ttgattctga actaaccgaa acattatact taccgcccca
     1021 ataaaactgt gagtaattgt aatcttttgg attaggcttt ttataacctg aattaatgtt
     1081 tccggctgcc ttaagtacta atgtatcttt gtcataactt ttatccttaa tgaaattaaa
     1141 cgttaaaatt tgagaaatat tcaatttatc attatcagat gttgctgttg ttttgtataa
     1201 agtgattttg tcatctactt ttttctcgct tacaggtgtg atatgttgag ctgcatcaac
     1261 tgtattcgat agcaaaagca gtgcaattga tgaagcaact gatgatttga ctaatttttt
     1321 cattttcata ttaattatgt cctttcactt taatttcgtg tgttttccaa ttaacttcat
     1381 atctaactac aaagtttcta tttacaaatg cattatgctt tctttctgcg taaataccag
     1441 ttctagggaa taaagtcgca tatgtaatat ctaagtttct accatatgaa atttcaaatt
     1501 cactcgtatc acttgaacct ttttcatgtg atagtgtagt gataaatgat ggattaaagc
     1561 cactttgaac taaaggtggc aattgattat caggagcaaa atattctctt gctgaacctg
     1621 ttggaccatt tggactttgt acgaataaat atctatcatg cgcagatttt ttaccatcag
     1681 gcgtaacaaa ttcgtttgct ttaacacccc atttaactga ttttgagttt tgcttgtcta
     1741 cttcactgac ataactcttt tgggtataac taattgtttt agaataatta aatgagccat
     1801 tgccacctat agatggtgct gactggaaat tacctccaat gttatatcct aatgtttgac
     1861 caacatcagt agtttctatt ttgtttttag gaaggtaatt gattaagcta acatttggat
     1921 ctttagtcgt cagtcctata ttatattgga atggccaaat cattcgttta gtcaattcat
     1981 atccactacc cttcacatct gaaaatgaag ttctggaatt aataaaacct tgcattttaa
     2041 caattaaagc atctttgtta tattttttat cttttacaaa gtcgaattga acattttgag
     2101 taacgcccca tttcttacta cttacatcct ccgtacgttt gattacttca gcaccatcac
     2161 caatattctc tatattagta ttagctctag attcttgaat cggagatgct aaaggcgcaa
     2221 tcagtcctac tgacaaagtt gcagctaaca tttttttctt aaacataagt ttcactttct
     2281 ttctatataa atttttaata caaatttatt ctagattatt tcacttattt ataaaattaa
     2341 ctgttcatta aaaaaataaa atgctataaa atattaaaaa tgatacaatt tattcattta
     2401 tgatgctgaa aaccggtcat tttggcgcaa ccttcattac atttaaatag ttccctttat
     2461 actcgaacaa tatgataaaa ttgccgatgc tcaacttttt cgctataaaa atcaaaacga
     2521 actttaatca aatcttaaaa aataatgttt gtattatcaa aaataatcta taatt
//
LOCUS       DAHHSC010000259         2558 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.263, whole genome shotgun sequence.
ACCESSION   DAHHSC010000259 DAHHSC010000000
VERSION     DAHHSC010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2558)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2558)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2558
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.263"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            207..527
                     /locus_tag="NBI57_002502"
     CDS             207..527
                     /locus_tag="NBI57_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000805733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF961 family protein"
                     /protein_id="HCD1823423.1"
                     /translation="MKPVLDIKKTLGQLNFLGVDEKYKYENGERTDKTVYAYKLASQE
                     QGEQITVKTPNKVELDYLQECELVEPDVKMYVQMSGDFGTIAYSWNAEDIKVVGSNSA
                     LKQK"
     gene            541..843
                     /locus_tag="NBI57_002503"
     CDS             541..843
                     /locus_tag="NBI57_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000386891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1823424.1"
                     /translation="MDYYTADRLYRYTNSSNLSEPILNYVASRINWGDKVSLMTLAKE
                     IQSKFNDSYVKENTVKGRPKIYADLCLLCMSLSEAGHGRMLQVNLEDCIYIGDIDV"
     gene            1018..2109
                     /locus_tag="NBI57_002504"
     CDS             1018..2109
                     /locus_tag="NBI57_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000172946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HCD1823425.1"
                     /translation="MTLKNQCTPLTNRGVERTNKSAVEAVVDWVQVTFHITPISAVIE
                     DVIGLPITLFKKRNSGIYFYNRGYEFSNIKLYYSSDDESMGIHLQLTGTGCREFEHHL
                     QQLNKTWQDFFDKCLSVNANFTRIDIAIDDYKTYLKVPLLIKKAEKAECVSRFRAGSA
                     INGFNLSDGRSKGATFYIGSKQSNLYCRFYEKNYEQAFKRHCDVEDIGLWNRYEIQMR
                     KAYAVNCAKVLSRTDNISEIVKSILHNNLRFISPPKDGNDKNRKRWPLYRPWALFIKD
                     TEKLNLTTRPTLKSIEDNLDWLCKQVATTLDTVLTAESMAQSEGLLTDTDFLDKILAH
                     SQFNDEHTNRINHYLEALKQKKHLSKDKC"
     gene            2170..>2558
                     /locus_tag="NBI57_002505"
     CDS             2170..>2558
                     /locus_tag="NBI57_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000691999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HCD1823426.1"
                     /translation="MKIISKYILTRFSKNGKHFDIPKEQRRVIPKFKNRRKMIVICFY
                     SLLAILLILLLASFIKATRANNDSKEAVNKTNMIQKKYEDNANTVQYSPKLKLYADKF
                     IDTYMNISKDSKELESREKELLKYFPS"
BASE COUNT          934 a          315 c          495 g          814 t
ORIGIN      
        1 agatgtatca tatttcaatt gaagacttag aattttttga ggtgatgaac cgataggttt
       61 gattaaaaat atgataggtc aggagaagca tagacactcc attatcaaaa atgcagtggt
      121 acggatttgc gggagttatt cctattcgtg cgacccttta gaagtcgttt aattttatta
      181 aaaattttgt aaaggatgga tatattatga aaccagtatt ggatattaaa aaaactttag
      241 gacaattaaa ctttttaggt gtagatgaaa aatataaata tgaaaatgga gagcgtactg
      301 ataaaactgt ttatgcatat aaattagcga gtcaagaaca aggcgaacaa atcactgtta
      361 aaacgcctaa taaagtggaa ttagactatt tacaagaatg cgaattagtt gaaccagacg
      421 ttaaaatgta tgttcaaatg tcaggcgatt ttggaactat cgcatatagc tggaatgcag
      481 aagatattaa agtagttggt tctaatagtg cacttaaaca aaaataatta ggtgatgtaa
      541 atggattatt atacagcaga tagattatat cgctacacaa atagttcaaa tttgagtgaa
      601 cctattttaa actatgttgc tagtcgtata aattggggcg ataaagtttc attaatgact
      661 ttagctaaag aaatacagtc taaatttaat gatagttacg ttaaagaaaa tacggtaaaa
      721 ggtcggccta aaatttacgc tgacctttgt ctattgtgta tgagcttgag tgaagcagga
      781 catggcagaa tgctacaagt taatttggaa gattgtatat atattggcga tattgatgtt
      841 taacatatag tattagtgat ttaacatgac cgtgtagctt ttagcgtatg caggcgcaga
      901 aatgcaagca cggagtgcgt taagcatttg ggcggagcct gcgacaaata cgcgttaaaa
      961 aagcgagaac ggtcatgtat caaatcgctt gcgatttgat aggaggcata aataaatatg
     1021 actttaaaaa accagtgtac cccccttact aatagggggg tagagcgtac aaataaaagt
     1081 gccgtggagg cagttgttga ctgggttcag gtcactttcc atataacccc tatttcagca
     1141 gtaattgagg acgttatcgg tttgcctata acgttgttca aaaaacgaaa tagtggcatt
     1201 tacttttata atcgcggtta tgaattttca aacattaaat tgtattattc aagtgatgat
     1261 gaatcaatgg gcattcactt acagttaact ggtacaggtt gtagagagtt tgaacatcat
     1321 ttacagcagt tgaataaaac ttggcaagat ttttttgaca aatgtttatc agttaatgca
     1381 aattttacaa gaattgatat tgcaattgat gattataaaa catatttaaa agtgccttta
     1441 ttaattaaaa aggctgaaaa agctgagtgt gtttcacgtt ttagagctgg aagcgcaata
     1501 aacggtttta atttgtcaga cggaagaagt aaaggcgcta ctttttatat tggttctaaa
     1561 caaagcaatt tatattgtcg tttttatgaa aagaattatg aacaagcgtt taaacgtcat
     1621 tgtgatgtcg aagacattgg gctatggaat cgttatgaaa tacaaatgcg aaaggcatat
     1681 gcagttaatt gtgcgaaagt tttaagtaga acagataata taagtgaaat tgtaaagtct
     1741 atattgcata ataatttgcg ttttatctct cctcctaaag atggaaatga taaaaatcgt
     1801 aagcggtggc cattatatag accttgggcg ttatttatta aagatactga aaaattgaat
     1861 ttaactacta gaccaacatt aaagtccatt gaggataatc ttgattggtt atgtaaacaa
     1921 gttgctacta ctttagatac agtgttaact gccgaaagta tggcgcaatc tgaggggttg
     1981 cttacagata ctgatttttt agataaaatt ttagcgcatt cacaatttaa tgatgagcat
     2041 acgaatagaa ttaatcatta tttggaggct ttaaaacaaa aaaagcactt atcaaaagat
     2101 aagtgttaaa aaaataactg ggatgtgatt tttattaaaa atttcgtaaa ggattttaga
     2161 aaatcgttta tgaaaataat aagcaagtac attttaacac gattttctaa aaacggaaag
     2221 cattttgata ttcctaaaga acaacgccga gtaattccaa aatttaaaaa tcgtcgtaag
     2281 atgattgtaa tttgttttta ctcattatta gctattttgt taattctttt attagcttct
     2341 tttattaaag ctacaagagc aaataatgac agtaaagaag cagttaataa aacgaatatg
     2401 attcaaaaaa agtatgaaga taatgcgaat acagttcagt atagtcctaa attaaagtta
     2461 tatgctgata agtttattga tacgtatatg aatatctcga aagattcaaa agaattggaa
     2521 tctagagaaa aagaattgtt gaaatatttt ccatcaga
//
LOCUS       DAHHSC010000260         2554 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.264, whole genome shotgun sequence.
ACCESSION   DAHHSC010000260 DAHHSC010000000
VERSION     DAHHSC010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2554)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2554)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2554
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.264"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            <1..722
                     /locus_tag="NBI57_002506"
     CDS             <1..722
                     /locus_tag="NBI57_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HCD1823427.1"
                     /translation="SFEAFKQLFNKDVHFNTFEVNTSLQSAKNTSVFIPSDVASYQYK
                     NIDEYVASIVSYIIEYTTITSSKCLVLFTSYKMMHMVQDMLNELPEFEDYVVLTQQQN
                     QNYKIVQQFNNFDKAILLGTSTFFEGFDFQANGIKCVMIAKLPFMNKHNAKYWLMDSE
                     FTSTFKEYVLPDAVTRFRQGLGRLIRNENDRGIIVSFDDRLINSNYKNFFEQTLENYR
                     QKKGDIQQFGKLLRQIQKKKK"
     gene            1044..2336
                     /gene="asnS"
                     /locus_tag="NBI57_002507"
     CDS             1044..2336
                     /gene="asnS"
                     /locus_tag="NBI57_002507"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HCD1823428.1"
                     /translation="MKTTIKQAKDHLNQDVTIGAWLTNKRSSGKIAFLQLRDGTGFMQ
                     GVVVKSEVDEEVFKLAKEIAQESSLYVTGTITEDNRSDLGYEMQVKSIEVISEAHDYP
                     ITPKNHGTEFLMDHRHLWLRSKKQHAVMKIRNEVIRATYEFFNKDGFTKVDPPILTAS
                     APEGTSELFHTKYFDQDAFLSQSGQLYLEAAAMAHGKVFSFGPTFRAEKSKTRRHLIE
                     FWMIEGEMAFTNHAESLEIQEQYVTHVVKSVLENCKLELKILERDTSKLEKVATPFPR
                     ISYDDAIEFLKAEGFDDIEWGEDFGAPHETAIANHYDLPVFITNYPTKIKPFYMQPNP
                     ENEETVLCADLIAPEGYGEIIGGSERVDDLELLEQRVKEHGLDEEAYSYYLDLRRYGS
                     VPHCGFGLGLERTVAWISGVEHVRETAPFPRLLNRLYP"
BASE COUNT          931 a          354 c          457 g          812 t
ORIGIN      
        1 attcgtttga agcttttaaa cagttattca acaaagatgt tcactttaat acatttgaag
       61 ttaacacgtc gttacaaagt gcaaaaaata caagtgtatt tataccgagt gatgtagcat
      121 cttatcaata taaaaatata gatgagtatg tagcatcgat tgtaagctat attattgaat
      181 atacgactat aacgtcatca aaatgtttag tcttatttac gagttataaa atgatgcata
      241 tggtacaaga tatgctaaat gaattgccag aatttgaaga ttatgttgta ttaacacaac
      301 agcagaacca aaattataaa atagtacaac agtttaataa ttttgataag gccatactgc
      361 ttggtacgtc aacatttttt gaaggttttg attttcaagc aaatggtatt aaatgtgtga
      421 tgatagcgaa gttaccgttt atgaacaagc ataatgcaaa atattggctt atggattctg
      481 aattcacttc aactttcaag gaatatgtat tacctgatgc agttacacgt tttagacaag
      541 gacttggaag attaattaga aatgaaaatg atcgcggcat aatagtttca tttgatgatc
      601 gactcataaa cagtaattat aaaaactttt ttgaacaaac acttgaaaat tatcgtcaga
      661 aaaaaggcga cattcaacag tttggaaaat tattaagaca aattcaaaaa aagaaaaagt
      721 gaaatacaaa gtaaaattct tgtgacatga catgtgctat gttctgttac aagaattttt
      781 gtttggtcgt caaaatttga gttttataat taaacttgaa tgttagcaca aaaaacgacc
      841 ttcatactaa taatattgag gctttattat atattttgtc tgaaactgat gattgtattg
      901 tcttaatata aattaatgtt caaatctgtg cgtaaatcat taccatttac acgttatctt
      961 ttagtctagt tctagaactt gaaagcggat tttgttaaaa tgaatgagta ttgtataacg
     1021 aaaaaataaa taggagaatg gttatgaaaa caacgattaa acaagcgaaa gatcatttaa
     1081 accaagacgt tacaattggt gcttggttaa caaataaacg ttcaagtggt aaaatcgcct
     1141 ttttacaatt acgtgatgga acaggcttta tgcaaggcgt agtagttaaa tcagaagttg
     1201 atgaagaggt attcaaactt gcgaaagaaa ttgctcaaga atcatctcta tacgttacag
     1261 gcacaattac agaagataat cgttctgact taggatacga aatgcaagtg aaatcaattg
     1321 aagttatttc agaagcgcat gactatccga ttacacctaa aaatcatggt acagaattct
     1381 taatggatca ccgtcattta tggttacgtt ctaaaaaaca acatgctgta atgaaaatta
     1441 gaaatgaagt tattcgtgca acgtatgaat ttttcaacaa agatggattt acaaaggttg
     1501 atccaccaat tttgacagca agtgcaccag aaggtacaag tgaattattc catactaaat
     1561 actttgatca agatgcgttt ttatctcaaa gtggtcagtt atacttagaa gctgcagcaa
     1621 tggcacacgg aaaagtattt tcatttggtc caactttcag agctgaaaaa tcaaaaacac
     1681 gtagacactt gatcgagttc tggatgattg aaggggaaat ggctttcaca aatcatgctg
     1741 aaagtttaga aattcaagaa caatatgtaa cacatgtagt aaaatcagtt ttagaaaatt
     1801 gtaaactaga gttgaaaatt ttagagcgtg atacatcaaa acttgaaaaa gttgcgacac
     1861 cattccctag aatttcatat gatgatgcaa ttgaattctt aaaagcagaa ggctttgatg
     1921 atattgaatg gggtgaagat tttggtgcgc cacatgaaac tgccattgct aatcattatg
     1981 atttaccggt gtttattact aattatccaa ctaaaattaa gcctttctat atgcaaccaa
     2041 atcctgagaa tgaagaaact gtcttatgtg cagacttaat tgcacctgaa ggatacggtg
     2101 aaattatcgg tggatctgaa cgtgtggatg acttagaatt gttagaacaa cgcgttaaag
     2161 aacatggatt agacgaagaa gcatatagtt actacttaga cttacgtcgt tatggtagtg
     2221 tgccacactg tggatttggt ttaggtttag agcgcacagt agcatggatt tctggtgttg
     2281 aacacgttcg tgaaactgcg ccattcccaa gattattaaa ccgtttatat ccataagttt
     2341 taggatcgtt acttaaaatt tatattatga taataaaaag aatcgtccag cctatattaa
     2401 tagctgggcg atttatgctt gcaaaatgaa gattgataac ggcttatatt aatcatgaac
     2461 aaacttttgt ttagtaacta tatttggaaa ataaatagtt catctgatta tccataaagt
     2521 tcattgttaa aatctagttt taaaaaatac tgtt
//
LOCUS       DAHHSC010000261         2553 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.265, whole genome shotgun sequence.
ACCESSION   DAHHSC010000261 DAHHSC010000000
VERSION     DAHHSC010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2553)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2553)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2553
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.265"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            <1..440
                     /locus_tag="NBI57_002508"
     CDS             <1..440
                     /locus_tag="NBI57_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HCD1823429.1"
                     /translation="IYDEYRSYLIEELDDCLTIQKNNDTAYYDVLEAINDLSNDSLCV
                     LNHLYINEGQEETFEQKFLQRNKHLKNVDGFKALRFLRPRTAGRHYIIITLWENRQAF
                     YHWQNSAEYKHTHKHRGTSKGADVKIINRELSYNIRIELADMV"
     gene            634..2232
                     /locus_tag="NBI57_002509"
     CDS             634..2232
                     /locus_tag="NBI57_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HCD1823430.1"
                     /translation="MQKSALFAPEKYNIISEIEKYSHIPDKKAILYHNTEGEDISVTY
                     QQLIEQSNKVGNVLASHGLSKGDKVLIMMPRSIATYELYIAALKLGVAIIPCSEMLRT
                     KDLQYRITHGEINAVIALEPFTVEFEKIKEYDALTKFVIAGQKDGWISLEAEKENASN
                     KLEMADTTRDDMAILSYTSGTTGNPKAVTHCHGWGFAHLQMAPKHWLCINEDDLVWAT
                     AAPGWQKWVWSPFLSVLGSGATAFVFNGRFSPETYLELLQKYQINVLCCTPTEYRMMA
                     KLQNLNDYDLTHLHSAVSAGEPLNREVVEQFKKYFNLTVRDGYGQTESTLLIGFLKDT
                     PQRIGSMGKGIPGSSVTVVDDEGNSVPANTKGNIAVPLDLPALFKGYYKEPERTAKAQ
                     TGKYYITGDLAHVDDDGYFWFEGRRDDIIISSGYTIGPFEVEDALTNHPAVKECAVVA
                     KPHDIRGNIVKAFVILQDHTAGDDTLVKELQQFVKNEVAPYKYPREIEFVDDLPKTNS
                     GKIRRVELRDSEIEKWQQQKDSNQ"
     gene            complement(2292..>2553)
                     /locus_tag="NBI57_002510"
     CDS             complement(2292..>2553)
                     /locus_tag="NBI57_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1823431.1"
                     /translation="EIQYLHPRKTLQLPLILKIILNLALIGIVIIVLTHTEFQLNAGD
                     SNVFLLNWDIRGLTSYIFSFLILVALVSKLILIFITRRQRTS"
BASE COUNT          918 a          386 c          497 g          752 t
ORIGIN      
        1 acatttatga tgaatataga agttatttaa tagaagaact ggatgattgt ctcacaatac
       61 aaaaaaataa tgacactgca tattacgatg tgctagaagc gataaatgat ttatcaaatg
      121 attcattatg tgtgttaaat catttatata ttaacgaagg acaagaagaa acattcgagc
      181 aaaaattttt gcaacgtaat aagcatttaa aaaacgttga tgggtttaaa gcattaaggt
      241 ttttaagacc tagaacagct ggtagacact atatcattat tacgctgtgg gaaaataggc
      301 aagcatttta tcattggcaa aattcagctg agtacaagca tacacacaag catcgtggca
      361 cttcgaaagg tgcagatgta aaaataatta atcgtgaatt atcatacaat attcgtattg
      421 aattagctga tatggtctag cccgagatat caaccctcaa tcatttgaaa gtccttgaat
      481 gctacgtcat ggcaaagtgt agtgccgagg actttggtat ttaaccctat ttaattgaaa
      541 attctgtaaa gataggcgta tgatatatat aattcattta taaaattaca atgttcatca
      601 cagggaaagt ggatgtatga aaggaagtta gttatgcaaa agtcagctct attcgcgcct
      661 gaaaaatata acattatttc ggaaatcgaa aaatatagcc acataccaga taaaaaggca
      721 attttatatc ataatactga aggcgaagat atatcagtca cttaccaaca gctcattgaa
      781 cagtctaata aagtaggtaa tgttttagca tcgcacggtt tatctaaagg cgataaagta
      841 ctcattatga tgccacgatc tattgcgacg tatgaattat atattgctgc tttaaaatta
      901 ggtgttgcaa tcattccatg ttctgaaatg ctaagaacta aagatttaca ataccgtatc
      961 acgcacggtg aaataaatgc agttattgca ctagagccat tcacagttga atttgaaaaa
     1021 attaaagaat atgatgcgtt aactaaattt gttattgccg gtcaaaaaga tggatggatt
     1081 tcacttgaag ctgaaaaaga aaacgcaagt aacaaactag aaatggcaga tacgacgcgt
     1141 gatgatatgg cgattctgtc atatacatct ggtacaactg gtaatcctaa agccgttaca
     1201 cattgtcatg gatggggatt tgcacattta caaatggcac caaaacattg gttatgtatt
     1261 aatgaagatg acttagtttg ggcaacagca gcaccaggtt ggcaaaaatg ggtatggagt
     1321 cctttcttat ctgtattagg ttcaggtgca actgcttttg tatttaatgg aaggttcagt
     1381 cctgaaacat acttagaatt gcttcaaaaa tatcaaatta atgttttatg ttgtacaccg
     1441 acagaatatc gtatgatggc taaattacag aacttaaatg attatgactt aacgcattta
     1501 catagtgccg tttctgcagg tgagccacta aatcgagaag tagttgaaca attcaagaaa
     1561 tactttaatt taactgtaag agatggatat ggccagactg aaagtacctt gcttataggc
     1621 tttttaaaag atacaccaca gaggattgga tcaatgggta aaggcattcc aggtagttcg
     1681 gtcactgtag tagatgatga ggggaatagc gtacctgcta atacgaaagg gaatattgca
     1741 gtaccacttg atttgccagc acttttcaaa gggtattata aagagccaga acgtacggcg
     1801 aaagcgcaaa caggtaaata ctacatcact ggtgacttag ctcatgtaga tgatgatggt
     1861 tatttctggt ttgaaggacg tcgagatgac attattatta gttcaggcta tacgattggg
     1921 ccttttgaag ttgaagatgc gttaacgaat catccagccg ttaaagaatg tgctgtagta
     1981 gcaaaacctc atgatatacg aggaaatatt gttaaagcat tcgttatttt acaggaccat
     2041 actgcgggtg atgatacgtt agttaaagaa ctacaacaat ttgttaaaaa tgaagttgca
     2101 ccatataaat atccgagaga gattgaattc gttgacgact taccaaaaac aaactcaggt
     2161 aaaattcgtc gtgttgaact acgagattct gaaatagaaa aatggcaaca acaaaaagat
     2221 agcaatcaat aaacatagca aagcagtcca atgcgtaagg tatgatgata cgcactggac
     2281 tgcttgttta tttatgatgt tctttgtcgt ctagttataa atattaaaat tagtttagaa
     2341 acaagcgcaa ccagtattaa gaatgagaaa atataagatg ttaaacctcg aatatcccag
     2401 tttaataaaa acacattact atcaccggca ttaagttgaa actcagtatg tgttagaacg
     2461 ataattacaa tacctataag tgcaagattc aaaataattt tcaatattaa aggtaattgt
     2521 aatgtttttc ttggatgcag atattgaatc tcc
//
LOCUS       DAHHSC010000262         2501 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.266, whole genome shotgun sequence.
ACCESSION   DAHHSC010000262 DAHHSC010000000
VERSION     DAHHSC010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2501)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2501)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2501
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.266"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            4..138
                     /locus_tag="NBI57_002511"
     CDS             4..138
                     /locus_tag="NBI57_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="delta-lysin family phenol-soluble modulin"
                     /protein_id="HCD1823432.1"
                     /translation="MSCLILRIFILIKEGVISMAQDIISTIGDLVKWIIDTVNKFTKK
                     "
     gene            complement(738..1523)
                     /locus_tag="NBI57_002512"
     CDS             complement(738..1523)
                     /locus_tag="NBI57_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen family hydrolase"
                     /protein_id="HCD1823433.1"
                     /translation="MKVQIYQLPIIFGDSSKNETQITQWFEKNMNAEVDVVVLPEMWN
                     NGYDLEHLNEKADNNLGQSFSFIKHLAEKYKVDIVAGSVSNIRNYQIFNTAFSVNKSG
                     QLINEYDKVHLVPMLREHEFLTAGENVAEPFQLSDGTYVTQLICYDLRFPELLRYPAR
                     SGAKIAFYVAQWPMSRLQHWHSLLKARAIENNMFVIGTNSTGFDGNTEYAGHSIVINP
                     NGDLVGELNESADILTVDLNLNEVEQQRENIPVFKSIKLDLYK"
     gene            complement(1884..2480)
                     /locus_tag="NBI57_002513"
     CDS             complement(1884..2480)
                     /locus_tag="NBI57_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002436950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HCD1823434.1"
                     /translation="MTFDNAMEERRSIYNLKDSISISDDELESMIAHAVKHVPSSFNS
                     QSTRIVLLLNDNNNKFWDNTKAILKEVMGENRDFEPTEQKIDNFKHSYGTILFYEDQD
                     VVSGLQEQMPNYYDNFAIWSTQTNAMHQFAIWTALATKGIGASLQHYNPLVDEMTSKE
                     FNIPKSWKLIAQMPFGDIREAAGEKTFNPVEDRFVIKK"
BASE COUNT          841 a          407 c          362 g          891 t
ORIGIN      
        1 ttgatgagtt gtttaatttt aagaattttt atcttaatta aggaaggagt gatttcaatg
       61 gcacaagata tcatttcaac aatcggtgac ttagtaaaat ggattatcga cacagtgaac
      121 aaattcacta aaaaataaga tgaataatta attactttca ttgtaaattt gttatcttcg
      181 tatagtacta aaagtatgag ttattaagcc atcccaactt aataaccatg taaaattagc
      241 aagtgagtaa catttgctag tagagttagt ttccttggac tcagtgctat gtatttttct
      301 taattatcat tacagataat tatttctagc atgtaagcta tcgtaaacaa catcgattta
      361 tcattatttg ataaataaaa tttttttcat aattaataac atccccaaaa atagattgaa
      421 aaaataactg taaaacattc ccttaataat aagtatggtc gtgagcccct cccaagctcg
      481 cggccttttt tattgtaata aagaagggga gagttaatcc tcattatgag acccaccgtt
      541 aaaatatatg aataagtcta atgttggaaa aggtcaaaaa attaatcaat ttaattaaga
      601 aaatcattca tttgcaaagg gcgaaatgga ttcttactga gttatctatt ataaaaaaat
      661 aaacatagac ttatgaaaaa tctctcataa atctatgttt agtcatgaca tgtgttaaat
      721 attatttcgg gcgcttctta tttatacaaa tctaatttaa tacttttaaa tacaggtata
      781 ttttcgcgtt gctgttctac ttcatttaag tttaaatcta cagtcaaaat atctgcggat
      841 tcatttaatt ctccaactaa atctccattt gggtttataa ctatcgaatg accagcatat
      901 tctgtgttac catcgaatcc agtgctatta gttccaatga caaacatatt attttcaatt
      961 gcacgtgcct ttagtaatga atgccaatgt tgaagacgtg acataggcca ttgcgccaca
     1021 taaaatgcaa ttttagcacc actacgagca ggatatctta ataattctgg aaaacgtaaa
     1081 tcataacaga taagttgggt cacataagta ccgtcagaca attgaaaggg ttcagctacg
     1141 ttttcgccag cggttaaaaa ttcatgctct cttaacatag gaactaaatg aactttgtcg
     1201 tattcattaa tcagctggcc acttttattc acactaaaag ctgtattaaa tatttgatag
     1261 tttctaatgt tagaaactga cccagctacg atatcgactt tatatttttc agctaaatgt
     1321 ttaataaatg aaaaactttg tcctagatta ttatctgctt tttcatttaa atgctctaaa
     1381 tcatagccat tattccacat ttcaggtaaa acgactacat ctacttcagc attcatattt
     1441 ttttcgaacc attgcgttat ttgagtttca tttttagaac tatctccaaa aataatcggt
     1501 aattgataaa tttggacttt cataacatcg catccttgat agatcttata tataacttac
     1561 taaaagttat gttgaaacgc aaaaaacgag cccaagacat aaaatcaaag tcctaggctc
     1621 tacaatgtta tattgacagt agttgatggg gccccaacat agagaaattg gaacaccaat
     1681 ttctacagac aatgcaagtt ggggtgggct ctaacataaa gaaatacttt ttctttagaa
     1741 attagtattt cttatacatg agttttactc atgtattcct attcttaagt gcacattagc
     1801 agcggctaat gtgtaagaac tactacataa tgaataacta atgattcttt atcatttctg
     1861 tcccattctt aacaatatat tgattatttt tttattacga aacgatcttc cactggatta
     1921 aatgtttttt cgccagcagc ttcacgaata tcaccaaatg gcatttgagc aataagtttc
     1981 caacttttag gaatattaaa ttcttttgaa gtcatctcat cgacaagtgg attatagtgt
     2041 tgtaatgaag cacctatgcc tttagtagct aatgcagtcc aaattgcaaa ttgatgcatg
     2101 gcatttgttt gagttgacca tattgcaaaa ttatcatagt agtttggcat ttgttcttgt
     2161 aaaccactta caacatcttg atcttcataa aacaaaattg taccgtatga atgtttgaag
     2221 ttatcaattt tttgttcagt tggctcgaaa tcacgattct ctcccatgac ttcttttaaa
     2281 attgcttttg tgttatccca aaatttatta ttgttgtcat ttaacaagag aacaattcta
     2341 gttgattgag aattaaatga tgaaggaaca tgtttaactg catgtgcaat cattgattct
     2401 aattcgtcat cgctaattga tatcgaatct ttcaaattat atattgaacg tctttcttcc
     2461 attgcattgt caaaagtcat tgctttttta tcttttttaa a
//
LOCUS       DAHHSC010000263         2465 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.267, whole genome shotgun sequence.
ACCESSION   DAHHSC010000263 DAHHSC010000000
VERSION     DAHHSC010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2465)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2465)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2465
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.267"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            complement(<1..487)
                     /locus_tag="NBI57_002514"
     CDS             complement(<1..487)
                     /locus_tag="NBI57_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HCD1823435.1"
                     /translation="MTGKTHASCGMLVGALTTQYFQTDIFSSVTVIILATLASLLPDI
                     CHTQSKIGRRFKVISFFVRLIFGHRTFTHSILFIAIIAFLLQIIQTPNYYMAAIIIGL
                     VSHVILDMITPRGVKLFYPLPFNVKTPIQFKTGGLVDLSLATALMVGTVYVLFQPFIN
                     DI"
     gene            664..924
                     /locus_tag="NBI57_002515"
     CDS             664..924
                     /locus_tag="NBI57_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1823436.1"
                     /translation="MAMTVKKDNNEVRIQWRVADIKIPTSEIKNITQDQDIHAVPKLD
                     SKDVSRIGSTFGKTNRVIIDTEDHEYIIYTQNDQKVYNELTK"
     gene            complement(1056..2426)
                     /locus_tag="NBI57_002516"
     CDS             complement(1056..2426)
                     /locus_tag="NBI57_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001670170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiH family protein"
                     /protein_id="HCD1823437.1"
                     /translation="MDSFSRKEIVIGRLKFITMSLIGILLFLVPIPVEQDGQKQTTLP
                     VAFLAGVLKDVLGGVMPFLIVTIITLSGIITLICSTILKDKLKPDGLMNNAFNVRIGW
                     LILRILAVVFAWMTFLRIGSKVIYSDETGGLLFSSLLPTLVAVFLFAALFLPLLMEYG
                     LLEMLGPIFRPVMRPLFTLPGRSTVDNLASFIGDGTVGVLITSRQYGEGYYSRREATV
                     ISTTFSVVSITFAIVVAETVHMQNQFFAFYLSVIVSCLVAAVIMPRIWPLNKIPDEYA
                     KEVPESARTEALPEGKTALRHGFDTATEVGIKAPGVIDFFKSGLKTVIDMWFVILPVV
                     MSIGTIATIIANYTPFFVILGKPFVPFLELMQIPEAAQASQTIIIGFADMFLPSILIE
                     GVQNDITRFVIGALSISQLIYLSEVGGVILGSKIPVSIGKLFMIFLIRTIITLPIISL
                     MAHLLL"
BASE COUNT          908 a          464 c          367 g          726 t
ORIGIN      
        1 ttatatcatt gatgaaaggc tgaaataaaa cataaactgt tccgaccatt aacgctgtag
       61 ctaaagataa gtctacaagt ccacctgttt taaattgaat cggtgtcttc acattaaacg
      121 gtaacggata aaataatttc acgcctcttg gtgttatcat atctagtata acatgtgaca
      181 ctaacccaat tataattgct gccatataat aattaggcgt ctgaataatt tgcaataaaa
      241 atgctatgat tgcgataaat aatattgaat gcgtaaaggt tcgatgtcca aagatcaatc
      301 tcacaaaaaa actaattacc ttaaaccttc tgccaatctt actttgagtg tgacatatat
      361 cgggtaataa gctagctaga gttgctagaa tgataaccgt aaccgacgaa aaaatatccg
      421 tttgaaaata ttgtgttgtt agcgctccaa cgagcatgcc acatgaagca tgtgttttac
      481 ctgtcatatt tgttctcctt taatactcac attttaccac atccctaaca aaaacacgaa
      541 catattttcg ggttaaaatt cattagtatg acacaattta aaaaagtatc acataactct
      601 tgaaaacgat tacaaaatcg tttatgatgt atttacaaaa tatttaaagg atgtgtttga
      661 ataatggcaa tgacagtaaa gaaagataat aatgaagtgc gtattcaatg gagagttgct
      721 gatatcaaaa ttcctacaag tgaaattaaa aatattacac aagaccaaga tattcatgca
      781 gttcctaaat tagacagcaa agatgtatct agaatcggct caacgtttgg taaaacgaat
      841 cgcgttatta tcgatactga agaccacgaa tacattattt atactcaaaa tgatcaaaaa
      901 gtttacaatg aattaactaa ataaattgta taaaaaaatc attcatggtg agggcttcat
      961 gaatgatttt tttaattgat tcaacaccca gcataaacaa atacaaaagg acaactgttc
     1021 tcataatttt aacagttgtc ctttttcaca tatatttata acaaaagatg tgccatcaaa
     1081 gaaataattg gtagtgtaat gattgttcta atcaagaaaa tcataaacaa tttgccgatg
     1141 cttacaggaa tcttcgaacc aagtatgacg ccacctactt cagacaagta tattaactgc
     1201 gatatactaa gtgccccaat aacaaaacga gttatatcat tttgtacacc ttcaattaat
     1261 atagaaggta aaaacatatc ggcaaaaccg ataataatcg tttgagaagc ctgtgccgct
     1321 tcaggtattt gcattaactc taaaaatgga acaaaaggtt tacccaatat gacaaaaaag
     1381 ggcgtgtagt tcgcaataat ggtagcaata gtaccaatac tcatcactac aggcaaaata
     1441 acaaaccaca tatcaatgac tgtttttaat cctgacttaa aaaagtcaat aacgcccggt
     1501 gctttaatac caacttctgt tgcagtatca aagccatgtc tcaatgccgt ctttccttct
     1561 ggcaatgcct cagtacgcgc actttcaggt acctccttag catactcatc aggaatttta
     1621 tttaaaggcc aaattcttgg cataatgact gctgcaacga ggcaggatac tatcactgat
     1681 aaatagaaag caaaaaattg attttgcatg tgcactgttt cagcaactac aattgcaaag
     1741 gtgatagaaa ctacactaaa tgtcgttgaa ataactgttg cctcacgacg agaataatat
     1801 ccttcaccat attgtctact tgtaattaag acaccaacag ttccgtctcc aataaatgat
     1861 gctaaattat ctaccgtcga acgtcctggc aatgtaaata aaggtctcat aaccggtcta
     1921 aaaataggac ccaacatctc taacaaaccg tattccatta atagcggtaa aaatagagct
     1981 gcaaataaga atactgccac taatgttggc aataaacttg agaacaataa tccacccgtt
     2041 tcatctgagt aaataacctt tgaaccaatt cgtaaaaatg tcatccatgc aaaaacaact
     2101 gctaatattc gtaaaattaa ccaaccaatt ctaacgttaa aagcattgtt cattagcccg
     2161 tcaggtttca atttatcttt taaaatagtt gaacaaatca gagttatgat acccgataaa
     2221 gtaattatcg tcacaattaa aaatggcatt acgccaccta atacatcttt aagcacgcct
     2281 gctaaaaatg ctacgggcaa cgttgtttgc ttctgtccat cttgttcgac tggaattggt
     2341 actagaaata ataagatacc aattaaagac atcgtaataa acttaagtct cccaataact
     2401 atctctttcc ttgaaaagct atccataaaa tcaatccatt tctctatgta ttcgttttaa
     2461 gtata
//
LOCUS       DAHHSC010000264         2455 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.268, whole genome shotgun sequence.
ACCESSION   DAHHSC010000264 DAHHSC010000000
VERSION     DAHHSC010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2455)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2455)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2455
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.268"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            <1..164
                     /locus_tag="NBI57_002517"
     CDS             <1..164
                     /locus_tag="NBI57_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="superantigen-like protein"
                     /protein_id="HCD1823438.1"
                     /translation="LYNGEFNKGQIKITADGNNYTIDLSKKLKLTDTNRYVKNPKNAQ
                     IEVILEKSN"
     gene            259..984
                     /locus_tag="NBI57_002518"
     CDS             259..984
                     /locus_tag="NBI57_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superantigen-like protein SSL13"
                     /protein_id="HCD1823439.1"
                     /translation="MNNNITKKIILSTTLLLLGTAFTQFPNTPINSSSEAKAYYINQN
                     ETNVNELTKYYSQKYLTFSNSTLWQKDNGTIHATLLQFSWYSHIQVYGPESWGNINQL
                     RNKSVDIFGIKDQETIDSFALSQETFTGGVTPAATSNDKHYKLNVTYKDKAETFTGGF
                     PVYEGNKPVLTLKELDFRIRQTLIKSKKLYNNSYNKGQIKITGTDNNYTIDLSKRLPS
                     TDANRYVKKPQNAKIEVILEKSN"
     gene            1092..1808
                     /locus_tag="NBI57_002519"
     CDS             1092..1808
                     /locus_tag="NBI57_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superantigen-like protein SSL12"
                     /protein_id="HCD1823440.1"
                     /translation="MSKNITKNIILTTTLLLLGTVLPQNQKPVFSFYSEAKAYSIGQD
                     ETNINELIKYYTQPHFSFSNKWLYQYDNGNIYVELKRYSWSAHISLWGAESWGNINQL
                     KDRYVDVFGLKDKDTDQLWWSYRETFTGGVTPAAKPSDKTYNLFVQYKDKLQTIIGAH
                     KIYQGNKPVLTLKEIDFRAREALIKNKILYTENRNKGKLKITGGGNNYTIDLSKRLHS
                     DLANVYVKNPNKITVDVLFD"
     gene            complement(2298..>2455)
                     /locus_tag="NBI57_002520"
     CDS             complement(2298..>2455)
                     /locus_tag="NBI57_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1823441.1"
                     /translation="FESESGYKISKNSGVPYQTVQDLRNGKTKLEDARFRTIIKLYSY
                     YVSLKEH"
BASE COUNT          910 a          380 c          357 g          808 t
ORIGIN      
        1 agttatataa cggagaattt aataaaggtc aaattaagat aactgctgat ggaaataatt
       61 acacgattga tttaagtaaa aagttaaaat taactgacac aaaccgttat gttaaaaatc
      121 ctaaaaatgc acaaattgaa gtcatactcg aaaaatctaa ctaacctatt accttttgta
      181 aatgcggata atttcaatta tctaattaac ccctttttat aattaaacat tccaacaata
      241 ctcaaaggag aaattcgaat gaacaataac atcacgaaaa aaattatttt atcaacaaca
      301 ttgttactat taggtacagc atttacacaa tttcctaata cacctatcaa ttcttcatct
      361 gaagcgaaag cttattatat aaatcaaaac gaaactaacg ttaatgagtt aactaaatat
      421 tactcgcaaa aatatttaac cttctctaac agtacgttat ggcaaaaaga taacggtacg
      481 attcatgcaa cgttgttaca gttttcttgg tatagtcata ttcaagttta tggacctgaa
      541 agttggggca atatcaacca attaagaaat aaaagcgttg atatttttgg cataaaagac
      601 caagaaacca ttgattcttt tgcattatct caagaaacgt ttactggtgg tgttactcct
      661 gcagcaacat ctaacgataa acactataaa ctgaatgtaa catataaaga taaagcagaa
      721 acgtttactg gcggatttcc agtttatgaa ggcaataagc ctgttttaac tttaaaagaa
      781 ttagattttc gtattcgtca aacattaatt aaaagtaaaa aattatataa taattcttat
      841 aataaaggac aaattaaaat aacaggtaca gacaataact acacaataga tttaagtaaa
      901 aggttgccat caactgatgc aaatagatat gttaaaaaac ctcaaaatgc aaaaattgaa
      961 gttatcctcg aaaaatcaaa ctaacaataa taatggagtt aataaaaata atcgcaaata
     1021 ctatattgac ttcgctcaca tttaaatttc ttattcctcg tatcatgatt cctctgaaag
     1081 gagatgttct aatgagtaag aacatcacga aaaatataat tttaacgaca acattattac
     1141 tattaggtac tgtattacct caaaatcaaa aaccagtatt tagtttttac tctgaagcta
     1201 aagcttatag cattggtcaa gatgaaacta acatcaatga attaattaaa tattacacac
     1261 agcctcattt ttcattttca aataaatggc tatatcaata tgataatgga aacatttatg
     1321 ttgaacttaa gagatattca tggtcagcac atatatcttt atggggcgct gaaagttggg
     1381 gaaatattaa tcagttaaaa gatcgttacg tagatgtgtt tggactaaaa gacaaagata
     1441 ctgatcagtt atggtggtct tatagagaga catttacagg tggcgttaca ccagccgcaa
     1501 aaccttctga taaaacttat aatctttttg tgcaatacaa agataaacta caaacgatta
     1561 ttggtgcgca taaaatatac caaggcaata aaccagtatt aacattgaaa gaaatcgatt
     1621 tccgtgcacg agaagcgtta ataaaaaata aaatattata taccgaaaat cgtaataaag
     1681 gtaagcttaa gatcaccggt ggcggtaata actacactat tgatttaagc aaaagattac
     1741 attcagatct agcaaatgtt tatgttaaaa atcctaataa aataactgtt gacgtcctct
     1801 ttgattagta tatgaaggtg acttatactt catgcacttt aattccaaat cagattattt
     1861 aaatgataat ttttaaagtg tatgatgtat ataataggtg aaattttcta tatatttaaa
     1921 tggaattggg agtaggaatg tgacagaaat ggtattttat aaaatttatt tcgttgtcac
     1981 tccccaactt gcattgtctg tagaatttct ttttgaattt ctctatgttg gggccccgcc
     2041 aacttgcatt gtctgtagaa tttctttttg aaattctctg tgttggggcc catccccaac
     2101 ttgcacatta ttgtaagctg acttttcgtc agcttctgtg ttggggcccc aaaaatgctt
     2161 gttacatggt cattttcatt cggtcaacta ctaccaatat aatattgtag agcctaagac
     2221 attgatttat tatgtcttag gctctattcc ttcatttaat gattcaatta ttatagcaat
     2281 actttattgt cccatgatta atgttctttt aatgagacat agtaactata aagtttaata
     2341 atcgttctaa atctagcatc ttctagtttg gttttcccat ttcttaaatc ttgtactgtt
     2401 tgatatggaa ctcctgaatt tttcgaaatt ttatagcctg attcagactc aaaca
//
LOCUS       DAHHSC010000265         2429 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.269, whole genome shotgun sequence.
ACCESSION   DAHHSC010000265 DAHHSC010000000
VERSION     DAHHSC010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2429)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2429)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2429
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.269"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            <1..105
                     /locus_tag="NBI57_002521"
     CDS             <1..105
                     /locus_tag="NBI57_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heptaprenyl pyrophosphate synthase subunit A"
                     /protein_id="HCD1823442.1"
                     /translation="MSDYYPSYFKKYNQSEVKHYLHDIQKSYLKSRGN"
     gene            108..833
                     /locus_tag="NBI57_002522"
     CDS             108..833
                     /locus_tag="NBI57_002522"
                     /EC_number="2.1.1.163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000774689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="demethylmenaquinone methyltransferase"
                     /protein_id="HCD1823443.1"
                     /translation="MADNKANKEQVHRVFQNISKKYDRLNNIISFEQHKVWRKRVMKD
                     MGVRKGTKALDVCCGTGDWTIALSKAVGPTGEVTGIDFSENMLEVGKEKTASMENVKL
                     VHGDAMELPFEDNSFDYVTIGFGLRNVPDYLVALKEMNRVLKPGGMVVCLETSQPTLP
                     VFKQMYALYFKFVMPIFGKLFAKSKEEYEWLQQSTFNFPGKEELKRMFEEAGFINVRV
                     RSFTGGVAAMHLGYKEKDNTKGD"
     gene            835..1794
                     /locus_tag="NBI57_002523"
     CDS             835..1794
                     /locus_tag="NBI57_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002463052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HCD1823444.1"
                     /translation="MPKLNMNNEIKKVEQRLEKAIKSKDSVLEQASLHLLSSGGKRVR
                     PAFVILSSQFGKDEQTSEQTYQVAVALELIHMATLVHDDVIDKSDKRRGKLTISKKWD
                     QTTAILTGNFLLALGLEHLMAVKDNRVHQLISESIVDVCRGELFQFQDQFNSQQTIIN
                     YLRRINRKTALLIQISTEVGAITSQSDKETVRKLKMIGHYIGMSFQIIDDVLDFTSTE
                     KKLGKPVGSDLLNGHITLPILLEMRKNPDFKLKIEQLRRDSERKEFEECIQIIRKSDS
                     IDEAKAVSSKYLSKALNLISELPDGHPKSLLLSLTKKMGSRNT"
     gene            1886..2335
                     /gene="ndk"
                     /locus_tag="NBI57_002524"
     CDS             1886..2335
                     /gene="ndk"
                     /locus_tag="NBI57_002524"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002463054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HCD1823445.1"
                     /translation="MERTFLMIKPDAVQRNLIGEVISRIERKGLKLVGGKLMQVPMEL
                     AETHYGEHQGKPFYNDLISFITSAPVFAMVVEGEDAVNVSRHIIGSTNPSEASPGSIR
                     GDLGLTVGRNIIHGSDSLESAEREINLWFNENEITSYASPRDAWLYE"
BASE COUNT          893 a          336 c          482 g          718 t
ORIGIN      
        1 atgtctgatt actatccatc atacttcaag aaatataatc aaagtgaagt aaagcattac
       61 ttacacgata tccaaaaaag ttatttaaaa agtagaggta attaagcatg gctgacaata
      121 aagcaaataa agagcaagta catcgcgttt ttcaaaatat ttcaaaaaaa tatgatagat
      181 taaataatat tattagtttt gagcagcata aagtatggag aaaacgcgtc atgaaagaca
      241 tgggtgttag aaaagggacg aaagcattag atgtttgttg tggtactggt gattggacaa
      301 tcgcattaag caaagctgta ggaccaacag gtgaagttac tggtattgac tttagtgaga
      361 atatgttaga agtaggaaaa gaaaaaactg cttcaatgga aaatgttaaa cttgttcatg
      421 gtgatgcaat ggaattgccg tttgaagaca attcttttga ttatgtaaca attgggtttg
      481 gattaagaaa tgtgccagac tatttagtcg cgttaaaaga aatgaataga gtacttaaac
      541 caggtggtat ggtggtatgt cttgaaacga gccaaccaac tttgccagtc tttaaacaaa
      601 tgtatgcact atactttaaa tttgttatgc ctatttttgg aaaattattt gcaaaatcaa
      661 aagaagaata tgaatggtta cagcaatcta cgtttaattt tcctggaaaa gaagagttga
      721 agcgcatgtt tgaagaagca ggtttcataa atgtaagagt acgtagtttt acagggggcg
      781 ttgctgcaat gcaccttggc tataaagaaa aagataatac caaaggtgat taacgtgcca
      841 aagttaaaca tgaacaatga aattaagaaa gtggaacaac gacttgaaaa ggcaataaaa
      901 agtaaagatt ctgtattaga acaggcatca ttacacttat tgtcttctgg tggtaaaaga
      961 gtacgcccag catttgttat tctgagtagc caatttggca aagatgaaca aacgtctgaa
     1021 caaacgtatc aagttgcagt cgcattagag ttaattcata tggcaacact tgttcatgat
     1081 gacgttattg ataaaagcga caagcgtcga ggcaagttaa ccatatcaaa gaaatgggat
     1141 cagacaactg ctattttaac tgggaatttt ttattggcat taggacttga acacttaatg
     1201 gccgttaaag ataatcgtgt acatcaattg atatctgaat ctatcgttga tgtttgtaga
     1261 ggggaacttt tccaatttca agaccaattt aacagtcaac agacaattat taattattta
     1321 cgacgtatca atcgcaaaac agcactgtta attcaaatat caactgaagt tggtgcaatt
     1381 acttctcaat ctgataaaga gactgtacga aaattgaaaa tgattggtca ttatataggt
     1441 atgagcttcc aaatcattga tgatgtatta gacttcacaa gtaccgaaaa gaaattaggt
     1501 aagccggtcg gaagtgattt gcttaatggt catattacgt taccgatttt attagaaatg
     1561 cgtaaaaatc cagacttcaa attgaaaatc gaacagttac gtcgtgatag tgaacgcaaa
     1621 gaatttgaag aatgtatcca aatcattaga aaatctgaca gcatcgatga ggctaaggca
     1681 gtaagttcga agtatttaag taaagctttg aatttgattt ctgagttacc agatggacat
     1741 ccgaaatcac tacttttaag tttgacgaaa aaaatggggt caagaaacac atagtattta
     1801 tgaaaagtat tgaaagcgct ttaccaacct gttaatatat aatagtaata tacataaacc
     1861 tattaaacac agggggatac ataaagtgga acgtacattt ttaatgatta aaccagacgc
     1921 agtacaaaga aatctaattg gtgaagtaat ttcaagaatt gaaagaaaag gactaaaact
     1981 tgtcggtggt aaattaatgc aagtaccaat ggaacttgct gaaacacatt atggtgaaca
     2041 ccaaggcaaa ccgttttata atgatttaat ttcatttatt acatcagcac cagtgttcgc
     2101 aatggtagtt gaaggtgaag atgcagttaa tgtatctaga catattattg gcagcaccaa
     2161 tccttcagaa gcttcaccag gatcaattag aggtgattta ggtttaactg ttggtagaaa
     2221 tatcattcac ggttcagatt cattagagtc tgctgaacgt gaaattaatc tatggtttaa
     2281 tgaaaatgaa attactagct atgcttcacc acgtgatgca tggttatatg aataaaatat
     2341 aaactgtaaa cctttacgat ttatttataa aggtagaaag ggttttgtta tgtggttagt
     2401 cattatgatt atacataaca aggcccgtt
//
LOCUS       DAHHSC010000266         2421 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.270, whole genome shotgun sequence.
ACCESSION   DAHHSC010000266 DAHHSC010000000
VERSION     DAHHSC010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2421)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2421)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2421
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.270"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            175..1398
                     /locus_tag="NBI57_002525"
     CDS             175..1398
                     /locus_tag="NBI57_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HCD1823446.1"
                     /translation="MTTFSEKEKIQLLADIVELQTENNNEIDVCNYLKDLFDKYDIKS
                     EILKVNEHRANIVAEIGNGSPILALSGHMDVVDAGNQDNWTYPPFQLTEKAGKLYGRG
                     TTDMKGGLMALVITLIELKEQNQLPQGTIRLLATAGEEKEQEGAKLLADKGYLDDVDG
                     LIIAEPTGSGIYYAHKGSMSCKVTATGKAVHSSVPFIGDNAIDTLLEFYNQFKEKYSE
                     LKKHDTKHELDVAPMFKSLIGKEISEEDANYASGLTAVCSIINGGKQFNSVPDEASLE
                     FNVRPVPEYDNDFIESFFQNIINDVDSNKLSLDIPSNHRPVTSDKNSKLITTIKDVAS
                     SYVEQDEIFVSALVGATDASSFLGDNKDNVDLAIFGPGNPLMAHQIDEYIEKDMYLKY
                     IDIFKEASIQYLKEK"
     gene            1842..>2421
                     /locus_tag="NBI57_002526"
     CDS             1842..>2421
                     /locus_tag="NBI57_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HCD1823447.1"
                     /translation="MNKVHKPLYFYLMLFFSTTIIGALLLYLPFTGKKPISFLDALFI
                     ASSAFTVTGLSPVDIGSQFNILGEIVILLLIQIGGLGIVTVTLLTLVFLNRKISMKNR
                     FLIMVTWNIDEPGGVIKLIKHLAIYSLVTELIGMICLCLSFIPKFGIGKGLFLSLFTS
                     VSAFNNAGFALFKNNLIDYSSDPIVIITISILI"
BASE COUNT          829 a          369 c          409 g          814 t
ORIGIN      
        1 tactataaaa ttaagcatac ccgatatcag tgttaataag atcgttgata ctagacagtt
       61 aatttcatag aacgaaatca aataacacgc tactttctgc attttaaatt atgtttaaga
      121 ataaaaatta tgtttatata aatatatata ctactttgaa aaggtgtgag cttaatgaca
      181 acttttagtg aaaaagaaaa aattcaatta ctagcagata ttgttgaact acaaactgaa
      241 aataataatg aaatagacgt ttgtaattat ttaaaagatt tattcgacaa gtacgatatt
      301 aaatctgaaa ttttgaaagt taatgaacac cgcgccaata tcgttgcaga aatcggtaac
      361 ggctcaccta tactcgcatt gagtggtcat atggatgttg ttgatgcagg aaatcaagat
      421 aattggactt atcccccttt tcaactcaca gaaaaagctg gcaaattata cggtcgaggc
      481 acaacggata tgaaaggcgg tctgatggct ttggtcatca cactcattga attaaaagaa
      541 caaaatcaat tgcctcaagg aacgataaga ttactagcta ctgctggcga agagaaagaa
      601 caagaaggtg ccaaattatt ggctgataaa ggctatttag acgatgtaga tggcttaatt
      661 attgctgaac caactggatc tggaatttat tatgcacata aagggtctat gtcatgtaaa
      721 gtaactgcaa ctggcaaagc tgtccatagc tcagttccat ttattggtga caatgcaatt
      781 gatacactgc ttgaatttta taatcaattt aaagaaaaat attcagagct taaaaaacat
      841 gatactaaac atgaattaga tgttgcaccc atgttcaaat cattgatagg aaaagaaatt
      901 tctgaagagg atgcaaatta tgcatctggt cttacagctg tatgttcgat tataaatggc
      961 ggtaaacaat ttaactctgt accagatgaa gcttcacttg aatttaacgt aagaccagtt
     1021 cctgagtatg ataacgactt tatagaatcg tttttccaaa atatcattaa tgatgtggat
     1081 agcaataagc tttcactcga tattccaagc aatcaccgac ctgtaacaag cgataaaaat
     1141 agcaaattaa ttactacgat taaagatgta gcttctagtt atgtagaaca agacgaaata
     1201 tttgtttcag cgcttgtagg tgcaacagat gcctctagtt tcttaggaga taataaggac
     1261 aatgttgatt tagccatttt tggaccaggt aatccattaa tggcacatca aatcgatgaa
     1321 tatattgaaa aagatatgta cctgaaatat attgatattt ttaaagaggc ttccattcaa
     1381 tatttaaaag agaaataaga acgatgctgt cagctgccct attcgcgtgc tggcagtttt
     1441 ttattgcgaa ttttaagttg atagaaatag cgatgaatat tgtttgactg taaatattca
     1501 aatatcaaca agcacatttt cattgattaa gtgatgtaaa actgaaatta tggtggtgat
     1561 ttgtcataca tatattgcat tgtctattga aattgggaat ccaatttctc tttgttgagg
     1621 cccccacccc aacttacatt gtttgtagaa tttcttttcg aaattctctg tgttggggcc
     1681 cccaccccaa cttgcattgt ctgttgaaat tgggaatcca atttctctgt gttggggccc
     1741 ctgactagaa ttgaaaagta atacacatca gtaacatttc tattttcatt tatgatatta
     1801 tctaattatt aatttataat ataaaggagt atgcaattaa aatgaacaaa gtccacaaac
     1861 ctttatattt ttatttaatg cttttcttct ctacaactat cataggtgct cttttattat
     1921 atttaccttt cactggtaaa aagccaatat cttttttaga tgccctattt atagcttcaa
     1981 gtgcatttac agttactggc ctatccccag ttgatatagg atcacagttt aatatacttg
     2041 gtgaaatagt aatactatta ttaattcaaa taggtggtct gggtatcgtg accgtaaccc
     2101 tattgacact agtattttta aatagaaaga tatcaatgaa aaatagattc ttgattatgg
     2161 ttacatggaa tattgacgaa cctggtggtg ttattaagct aattaaacac ttggctattt
     2221 atagtttagt cactgaatta attggtatga tttgtttgtg tttatctttt ataccaaaat
     2281 ttggtatagg caaaggttta tttttaagct tattcacatc agtatcagct tttaataatg
     2341 ctggatttgc cctttttaag aataacttaa tagattattc tagtgatcca attgtcatta
     2401 ttacaatctc aatactcata a
//
LOCUS       DAHHSC010000267         2370 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.271, whole genome shotgun sequence.
ACCESSION   DAHHSC010000267 DAHHSC010000000
VERSION     DAHHSC010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2370)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2370)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2370
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.271"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            99..1481
                     /locus_tag="NBI57_002527"
     CDS             99..1481
                     /locus_tag="NBI57_002527"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HCD1823448.1"
                     /translation="MKVSKFGGSSVSNATQIKKVLNIVNSDPERKIVIVSAPGKRHDN
                     DIKTTDLLIRLYEKVINHLDYHDKKREIIQRYDDIVKELQMDESILRTIDVTLEHYIN
                     QLKNEPKRLLDALLSCGEDFNAQLIALYNNSQGIPTKYISPKEAGIFVTDVPQEAQIL
                     DSAYSEIKKLRDYDEKLIIPGFFGVSHEGYIVTFPRGGSDITGAIISSGVRATIYENF
                     TDVSGIYKANPNIIKDPELIEEITYREMRELSYAGFSVFHDEALQPLYKDRIPVVIKN
                     TNRPQDKGTFIVHDREINAKNVISGISCDKDFTVINIKKYLMNRQVGFTRKILGVLED
                     NNISFDHMPSGIDTISIVMRSKQIQNKETKVLNEIRQKCDVDELSIEHDLAILMIVGE
                     GMNKVIGTANKITHALAESKINLKMINQGASEISMMFGIHVDDAEKAVLSTYEFCYHG
                     VALKNLCDKN"
     gene            complement(1535..2110)
                     /locus_tag="NBI57_002528"
     CDS             complement(1535..2110)
                     /locus_tag="NBI57_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1823449.1"
                     /translation="MNAKEQLVDNLMKTSSQLFKFHGEVAMQLFLNDELKLPSIVEIC
                     VERKRLSDIVKVIPQSYALLYIDKQDQAIAKEDLSLSKIAKVYVQYDDTTIMSIFVYD
                     VVNDEWIFRLDPNIRIPKSNIYFHSLNWDVDYIKPEIVLMYDLMQHHQYHHYSNYKRV
                     IDALSYYQFFILKFVVGEQRIKDAIQRTINN"
BASE COUNT          858 a          349 c          368 g          795 t
ORIGIN      
        1 gtaaagttcg taatattcaa aatataaata cataatatca ttcttactta caaaactgaa
       61 tacaattttc tgatttttta gaaaggttgg catgcactat gaaagtttcc aaattcggtg
      121 gtagctccgt ctccaatgct actcaaatta aaaaggtttt aaatattgtt aattctgatc
      181 cagagcgaaa gattgttatc gtttctgctc caggtaaaag acatgataat gatattaaaa
      241 caactgattt gttaatcaga ttatatgaaa aggtcattaa tcatcttgat tatcatgata
      301 aaaaaagaga aattattcag cgttatgatg atattgtaaa agaattgcaa atggatgaaa
      361 gtattttacg gacgatagat gtgactttgg aacattatat taatcaatta aaaaatgaac
      421 caaagagact attagatgca ttactttctt gtggtgaaga ttttaatgcg caattgatag
      481 ctttatataa taatagtcaa ggtataccaa caaaatatat atcaccgaag gaagcgggta
      541 tttttgttac tgacgtccca caagaagcac aaatattaga tagtgcttac tctgaaatta
      601 aaaaattaag agattatgat gaaaaactaa ttatcccagg attttttggc gtatctcatg
      661 aaggatatat agttacattt ccacgtggcg gatcagatat aactggcgcc atcatatcaa
      721 gtggtgttag agccacaatt tatgaaaatt tcaccgatgt gtctggtatt tataaagcta
      781 atccaaatat catcaaagat cctgaattaa tcgaagaaat cacttatcga gaaatgcgtg
      841 aattatctta tgctggtttt agtgttttcc atgatgaagc cttacaacca ctttataaag
      901 atcgcatccc tgtggtgatt aaaaatacga accgccctca agataagggt acatttatag
      961 tgcatgatag agaaataaat gcgaaaaatg tgattagcgg tataagttgt gataaagatt
     1021 ttactgtcat taatatcaaa aagtatttaa tgaatagaca agtcggcttt actagaaaga
     1081 tattaggtgt cttagaggat aataatattt catttgatca tatgccttca ggtattgata
     1141 ctatcagtat cgttatgcgt tcaaaacaaa ttcaaaataa agaaacaaaa gtattaaatg
     1201 aaattcgtca aaaatgtgat gttgatgagc taagtattga gcatgattta gcaatattaa
     1261 tgattgtcgg cgaaggtatg aataaagtca tcggtactgc aaataaaatt acacatgcct
     1321 tagctgaatc aaaaattaac ttaaaaatga ttaaccaagg tgcttcggaa atctcgatga
     1381 tgtttggaat tcatgtagat gatgctgaaa aagctgtatt atctacatat gagttttgct
     1441 atcacggggt tgcattaaaa aacttgtgtg ataaaaacta atataaaagc aattcgtaac
     1501 acatgtattc aactgcgtta tgaattgctt tttcttaatt atttattgtt ctctggattg
     1561 catccttaat acgttgctca cctactacaa attttaaaat aaaaaattga tagtagctta
     1621 atgcatctat gactcgttta taattggaat aatgatgata ctgatggtgt tgcattagat
     1681 catacattag aacgatctcc ggtttaatat aatccacatc ccaatttaaa ctatggaagt
     1741 atatgttact cttaggtata cgtatattcg gatccaatct aaaaatccat tcatcgttta
     1801 ctacatcata aacgaaaata ctcattattg ttgtatcatc atattgcaca taaacttttg
     1861 caatttttga aagtgataaa tcttctttag ctattgcttg atcttgctta tctatgtata
     1921 gtaacgcata tgattgcgga ataactttca caatatcact taaacgctta cgttccacgc
     1981 atatttcaac aatagaaggt aattttaatt catcatttaa gaaaagctgc atggcaactt
     2041 caccgtgaaa tttaaataat tgcgatgatg ttttcattaa attgtccact aattgttctt
     2101 tagcattcat ttcattcact ctcaaataca cctcctaagc aagcgctttc acaaatatca
     2161 ttatacattt taaaattaat gaattcaatc ttcaaaatac tatgagtgac ctaattctgt
     2221 catttaaatg accgttataa cataaattcg acataaactt gaattcacga atctttcacg
     2281 tcatattcat tgaacatcaa tatctgtcag tttataattt gattataaca atgatgaact
     2341 aaaatcaact aataaaaatg tgggaaattg
//
LOCUS       DAHHSC010000268         2340 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.272, whole genome shotgun sequence.
ACCESSION   DAHHSC010000268 DAHHSC010000000
VERSION     DAHHSC010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2340)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2340)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2340
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.272"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            <1..794
                     /locus_tag="NBI57_002529"
     CDS             <1..794
                     /locus_tag="NBI57_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HCD1823450.1"
                     /translation="SKRLIVPYMIFFAFFSIYYFLTGKSDELQLDPFNPVFALWFLIT
                     LFFFHVILVIVRRFNPYKVLSVSIIISIGAGFSDNIDSYLSISRTIVFFPIFYLGYIF
                     TKKHTAIFKNKKLIPVSIITFILFFIIYVIHPINADWLLGSSPYTSLENEGQSIFSPF
                     KRLILYGIILIAMTAFLNLMSTKKKLYTYIGSRTLYVYLLHGLIIGIVRGFEWYPFDN
                     PISLMTYLYLISISVIIVYVLSTNFVCKWTNPIINLQRPSQFKDS"
     gene            990..2144
                     /locus_tag="NBI57_002530"
     CDS             990..2144
                     /locus_tag="NBI57_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HCD1823451.1"
                     /translation="MKLSLNSNSKYLRAPSIRQFSNRMNNLDDCVNLTIGQPDFPMPD
                     VVKKAYIDAINNDKTSYSHNKGLLETREAISQYFKNRYHFSYDPEEIIVTNGASEAID
                     TTLRSIIDPGDEIIIPGPIYAGYIPLIEVLGGKPIYIDTTATQFKITPDALESHISPK
                     TKAVLLNYPTNPTGVVLKRNEVLNIVNVLKKYPIFIISDEIYAENTFSGKHVSFAEFE
                     DIRDQLILIGGLSKSHSATGIRIGFLLGPQYLIDKLTFMHAYNCICANVPAQIACITA
                     LNEGLEAPKYMNEAYVERRNYLVSELTKLGFEITAQPEGAFYIFPSIKHITDDDFEFC
                     VDLLESTHLAIVPGSSFTEFGKGFVRISYAYEMDVLKEGMKRLAKYLNTK"
BASE COUNT          806 a          351 c          335 g          848 t
ORIGIN      
        1 tttcaaaacg tttgatagta ccttatatga tattttttgc attcttttca atatattatt
       61 tcttaacagg taaaagtgac gaattacaac ttgatccttt taacccagta tttgcgttat
      121 ggtttttgat cacgctattt ttctttcatg tcattttagt aatagttaga agatttaatc
      181 catataaagt gctatcagtt tcaattatta tatctattgg agcaggtttt tcagataaca
      241 tcgatagcta cttgagcatt tcaaggacaa tcgtattttt cccaatattt tacctaggtt
      301 acattttcac caaaaaacat acggctattt ttaaaaataa aaaactaata ccagtatcta
      361 tcattacctt tattttgttt tttatcattt acgtgattca tcctatcaat gcagattggt
      421 tattgggaag ttcaccatat acttcacttg aaaatgaagg acagagtata ttcagtcctt
      481 ttaaacgact tatattatat ggaattatat taatcgcaat gactgcgttt ctaaatttaa
      541 tgtcaacaaa gaaaaaatta tatacttata ttggtagtcg tacgctatac gtatatttat
      601 tacatggttt aattattggc attgttagag gatttgaatg gtacccattc gataatccta
      661 tatcattaat gacttatctt tatttaataa gtatatctgt aatcatcgta tatgtattat
      721 ctacaaactt tgtttgtaaa tggacaaatc caataattaa tttacaaaga ccatcacagt
      781 ttaaagattc ataattttat agtgtctaag tacattaact gtaaccactt tattcatttt
      841 gaaaagtagt tactacagtg tatttagaca tttttcgttt ttcaaataaa gcttaagtaa
      901 tttacccttt tttggtttaa aagtatgtta tgatattatt aatttctaaa tttttagaca
      961 atatttgatt tatatttgag gtgtcacaaa tgaaactttc tttaaattct aattctaaat
     1021 atttaagagc accaagtatt cgccaatttt caaatcgtat gaataatttg gatgattgtg
     1081 ttaatttaac tattggccaa ccagacttcc cgatgcctga tgttgtgaaa aaggcatata
     1141 ttgatgctat taacaatgac aagacatcat actctcacaa taaagggtta ttagaaactc
     1201 gcgaagcaat tagtcaatac ttcaaaaatc gttatcattt ttcctatgat cctgaagaaa
     1261 ttattgtgac aaatggagca agtgaagcaa tagacacaac gttacgttct atcatagatc
     1321 ctggagatga aattataata ccgggaccaa tttatgcagg ctacatacca ctcatcgaag
     1381 tactaggtgg taaaccaatt tatattgata caacagcaac acaattcaaa attacacctg
     1441 atgcattaga aagtcatatt tctccaaaga caaaagctgt cttgttaaat tatccgacta
     1501 atccaactgg cgtagtttta aaaagaaatg aagttttaaa tatcgtaaat gtattaaaaa
     1561 aatatccgat atttattatt agcgatgaga tttatgctga aaatacattt agtggtaaac
     1621 atgtatcctt cgctgaattt gaagacattc gtgatcaatt aatattaatt ggtggtttaa
     1681 gtaaatcaca ctcagcaacc ggaataagaa ttggttttct attagggcct caatatttga
     1741 ttgataaatt aacatttatg cacgcctata attgtatttg tgccaatgtt ccagctcaaa
     1801 tagcatgtat aacagcactt aacgaaggat tagaagctcc taaatatatg aacgaagctt
     1861 atgtagaacg aagaaattat ttagtatctg aattaactaa attaggtttt gagattaccg
     1921 ctcaacctga aggcgcgttt tatattttcc caagtattaa acatatcacc gatgacgatt
     1981 tcgaattttg tgtcgattta cttgaatcaa cacatttagc aatcgttcca ggctcatcct
     2041 ttacagaatt cggtaaagga tttgtaagga tttcctatgc ttatgaaatg gatgttttaa
     2101 aagaaggtat gaagagactt gctaaatatt taaatactaa ataaaaatga tatgttgaac
     2161 tggacgtcgt gaactaaatt catagtacgt tcagtttttt atattctata agttttaaat
     2221 aatactctat agagactgta aatactaccg agttatattc ctctccttta aacacctcac
     2281 tcaaaatgat tctatcaaaa ggttttaata tgctaactta taattgttct tcgaaactta
//
LOCUS       DAHHSC010000269         2294 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.273, whole genome shotgun sequence.
ACCESSION   DAHHSC010000269 DAHHSC010000000
VERSION     DAHHSC010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2294)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2294)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2294
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.273"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            complement(128..454)
                     /locus_tag="NBI57_002531"
     CDS             complement(128..454)
                     /locus_tag="NBI57_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HCD1823452.1"
                     /translation="MILDNVNPNDLFPTEKKGPSVLGIIEYQVQGENEFEGAFIATNE
                     RLIMNVDMNGQFYYRSISYNEIEKIDYDGQTIMFKFNIGNVPMHDIKSDNVEMFVEYV
                     KQHMIV"
     gene            complement(518..1261)
                     /locus_tag="NBI57_002532"
     CDS             complement(518..1261)
                     /locus_tag="NBI57_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SarA family transcriptional regulator"
                     /protein_id="HCD1823453.1"
                     /translation="MSKFKVKRLTAHILMLKVINDMLKYSFHLTLTQVKLLNLLVKYD
                     NSDSNSASIDRLLKMKEIQSKMALLQMLSYLNNHQWLLKGRDQRDQRKLTISINKMHI
                     DKIEYMNNELSDYIERYFGQYTFDFSCDYVSYLLSSQELFRNLKCYLNMCQLTLEELY
                     VLGILNLHKGQLTVKELQGEFHHPIFAVSPILKCLILKGLVKKERCELDERRVIVTIK
                     REKFSKVTMLIQACYNYLEKGIQVKLNNK"
     gene            complement(1287..>2294)
                     /locus_tag="NBI57_002533"
     CDS             complement(1287..>2294)
                     /locus_tag="NBI57_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HCD1823454.1"
                     /translation="QIIQRLGVNLENLHIIVTIDTSNELSIEQITLLLQKIVNYNVNV
                     VFRIKHEYKELLTSTERGKIEKLVATIFNMTFESNRCKIAFLINDLNTTAIQKLKRLI
                     DNYIGVFELIYHLEPKELNTHYDKEIDHLIDYFILPLRQIEQVSIACSKIICDVDLIN
                     ENNDLKEKITMSDFNKVTELLTNYTKFRGLIMHMSIISEASKEINLTRLSIFIFIINI
                     LNQLRGIVIYQNDTMIVTKFKHEVQCVICLPAALLNENSDAIRLSCKGLQQFSHAKTK
                     QIVLSIDKDNHTSENGVLVNDIMQQHSYWKANFIERNIYDHSLMVSSNSIVHLKYHLE
                     I"
BASE COUNT          777 a          388 c          252 g          877 t
ORIGIN      
        1 gatggcttgc tcacaaaatt tataaaatat aaaaaaagaa gagctttaaa tcataacgca
       61 tgccatttat ctttaaatgc aatgagttat cattcaaact cttcttatca acctacatac
      121 attgacatta tactatcata tgttgtttta catattcaac aaacatttca acgttatcac
      181 ttttaatatc atgcattggg acattaccaa tattaaattt gaacattata gtttgaccat
      241 cataatctat cttctctatt tcattataac taatacttct ataataaaat tggccattca
      301 tatctacatt cataattaat ctttcatttg ttgctataaa tgccccttca aattcattct
      361 caccttgaac ttgatactca attattccta gcactgacgg accctttttt tcagttggaa
      421 ataaatcatt cggattcaca ttatctaaaa tcataatatc cctcccactt aaaactataa
      481 actattcttc ataggtatat gaaatacaag tgattaacta tttattatta agcttaactt
      541 gtattccctt ttcaagataa ttataacatg cttgaataag catagtgact ttagaaaact
      601 tttctcgctt gattgttaca ataacacgtc tttcatctaa ttcacatctc tcttttttaa
      661 ctaatccctt caaaattaaa cactttaaaa tcgggctaac cgcaaatatc gggtgatgga
      721 attcaccctg taattcttta accgtcaatt gacctttatg cagatttaat attcctaaaa
      781 cgtataactc ttctaatgtt aattgacaca tatttaaata acactttaga tttcgaaata
      841 attcttgtga cgacaataaa tacgatacat agtcacaact aaaatcaaat gtatattgac
      901 caaaataacg ttcaatatag tcactaagct cattattcat atattctatt ttatctatat
      961 gcattttatt aatcgaaata gtcaatttac gttgatccct ttgatctctt cctttcaata
     1021 gccattgatg attgtttaaa taacttaaca tttgaagaag tgccatctta gattggattt
     1081 ctttcatttt taataaccta tctattgaag cgctattgga atcagaatta tcatatttaa
     1141 ctaatagatt taatagtttc acttgagtca aagtgagatg aaatgaatat ttcaacatat
     1201 cattaatgac tttcaacatt agtatgtgtg cagttaaacg cttaacttta aatttagaca
     1261 tctttaaaac ctctcttaaa ccatgcctat atctcaagat gatatttcaa atgaacaata
     1321 ctattgcttg agaccattaa tgaatgatca taaatatttc tttctataaa attagctttc
     1381 caataactgt gttgttgcat aatatcattc acaagtacac cattttcgga agtatgatta
     1441 tctttatcta tacttaaaac aatttgttta gttttagcat ggctaaattg ttgaagaccc
     1501 ttacacgata aacgtatagc gtctgaattc tcatttaaca atgcggctgg caaacaaatg
     1561 acacattgta cttcatgttt gaacttcgtt acaatcatcg tgtcattttg ataaataaca
     1621 atccctcgta attgattaag tatatttatg ataaaaatga atattgataa tctagtcaaa
     1681 tttatttctt tgctagcttc acttataatt gacatgtgca taataagtcc tcgaaatttt
     1741 gtataatttg tcaaaagttc agtaacctta ttaaaatcag acattgtgat tttttctttt
     1801 aagtcattat tctcattaat caaatctaca tcacatataa ttttagaaca tgcaatagac
     1861 acctgttcta tttgtcttag cggtaagata aaatagtcta ttagatgatc aatttcttta
     1921 tcatagtgcg tattcaactc ttttggttcc agatgatata ttaattcaaa tactcctata
     1981 taattatcta ttaagcgttt cagtttttga attgcagtag tatttaagtc gttaattaaa
     2041 aatgcaatct tacatctatt actctcaaat gtcatattaa aaattgtggc tactagcttt
     2101 tctatcttgc ctctttcagt actcgttaac aattccttat attcatgttt aatacgaaat
     2161 actacattta cattgtaatt cactatcttt tgaagtaata aagtgatttg ttcaatcgat
     2221 agctcattgc ttgtgtcgat tgtaacaatt atatgcaagt tttcaagatt aacacctaat
     2281 ctttgaatga tttg
//
LOCUS       DAHHSC010000270         2277 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.274, whole genome shotgun sequence.
ACCESSION   DAHHSC010000270 DAHHSC010000000
VERSION     DAHHSC010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2277)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2277)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2277
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.274"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            16..381
                     /locus_tag="NBI57_002534"
     CDS             16..381
                     /locus_tag="NBI57_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3139 domain-containing protein"
                     /protein_id="HCD1823455.1"
                     /translation="MKKKKNAIKVIIILFISLIVAVAFFFGLKTYQGHKNIQLIDSYL
                     EEKNLKDKIKSEKTEYSAKKGLFYKEVTFKDEPGVTYVVQPISTNKGLFVEGFDTETK
                     KSLKTAKHKYFNQNYKPSK"
     gene            563..1840
                     /locus_tag="NBI57_002535"
     CDS             563..1840
                     /locus_tag="NBI57_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:dicarboxylase symporter family
                     transporter"
                     /protein_id="HCD1823456.1"
                     /translation="MALFKRKISLPMQVVIALVLGVVVGLLLYGQENVANYIKPFGDV
                     FLNLIKMIVIPVVFCSLALSISNVGESKTVGRYGWKTILYFEIITTIAIGLGIIFGNL
                     FKPGAGLDPTKLPKGDISKYQSTAHAAEQSTYGNHFIDTIVHIIPTNFFEALNKGELL
                     PIIFFAVFFGLGLAAVGKKAEPVKEFLSGSLEAVFWMINKILKLAPLGVFAFICTTII
                     TFGASALLPLLKLVLVVVFAMVFFVFAILGLVAWMCGINIMNIIRILKSELLLAFSTS
                     SSEAVLPVMMKKMENFGSPKEITSFVIPIGYTFNLDGSALYQSIAALFVAQMYGMHLT
                     LSEQIVLMLTLMITSKGMAAVPGTSIVVLLTTLGAMGLPAQGLALIIGVDRILDMVRT
                     CVNVIGNALSTIVIAKWENVYDKAKGQEYLKSI"
     gene            complement(2097..>2277)
                     /locus_tag="NBI57_002536"
     CDS             complement(2097..>2277)
                     /locus_tag="NBI57_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4889 domain-containing protein"
                     /protein_id="HCD1823457.1"
                     /translation="KNVKLPSDSDVKVKKGDFVIVYKLADSDKIVKVKKVDHDDVPHG
                     LMMKIHDMGKMHMKH"
BASE COUNT          776 a          326 c          398 g          777 t
ORIGIN      
        1 aaaaggagtt acataatgaa gaagaaaaaa aacgcgatta aagttatcat tatattattt
       61 atttcattaa ttgttgcagt agcatttttc tttggtctga aaacatatca aggccataag
      121 aatattcaac taattgacag ttacttggaa gagaaaaatc taaaagataa aattaaatca
      181 gaaaaaacag agtatagtgc taaaaaaggt ctcttctata aggaagttac gtttaaagat
      241 gaacctggtg taacttatgt tgtgcaacca atcagtacta ataaaggatt atttgttgaa
      301 ggctttgata cagagactaa gaaaagtttg aaaactgcaa agcataaata tttcaatcaa
      361 aattataaac catcaaaata gtgaaacgtt aaactggtga aacagtttag tgccttcaat
      421 tttaattatt tgaggtaaac agtttttgta tggaaattag tgatttttga catctataaa
      481 agataaatat tttaattttt tatagataat tatcaatttt ctgatataat tgagtgatgt
      541 cagatgaagg ggagtttgta ctatggctct attcaagaga aaaattagcc tacctatgca
      601 agttgttatt gcattagtgt taggtgttgt cgtaggactt ttattatatg gacaagaaaa
      661 cgtagcaaat tacattaaac catttggtga cgtattttta aatttaatta aaatgatcgt
      721 tataccagtt gtattttgct cactagcgct ttctatttcg aacgttgggg aatcgaaaac
      781 tgtagggcgt tatggctgga aaacaatttt atactttgaa attattacaa caatcgcaat
      841 aggtttaggg attatcttcg gtaacctatt taaaccaggt gctggattag acccaacaaa
      901 attacctaaa ggtgatattt ctaaatatca atcaactgca catgcagcag aacaatctac
      961 atatggaaat cattttattg ataccattgt acatattatt ccgacaaact tttttgaagc
     1021 tttaaataag ggtgaattat tacctattat cttcttcgca gtattctttg gattaggatt
     1081 agctgctgta ggtaaaaaag cagaaccagt taaagaattt ttaagcggat cgcttgaagc
     1141 tgtgttctgg atgattaata aaattttaaa attagcacca cttggagtgt ttgcattcat
     1201 ttgtactaca attattacat ttggtgcatc cgcattatta ccactattaa aattagtatt
     1261 agttgttgtc tttgcaatgg tgttctttgt attcgctata ctaggactag ttgcatggat
     1321 gtgtggtatt aatatcatga atattattag aatcttgaaa agtgaattgc ttttagcatt
     1381 ttctacatca agttcggaag ctgtacttcc tgtaatgatg aagaaaatgg aaaacttcgg
     1441 ttctccaaaa gaaattactt cttttgttat accaattggt tatacgttta acttagacgg
     1501 atcagcactt tatcaatcta ttgcagcatt attcgttgca cagatgtatg gaatgcactt
     1561 aacattatca gagcaaattg tgttgatgtt aacattaatg attacgtcta aaggtatggc
     1621 ggcagtacca ggaacttcaa tcgttgtttt attaacaaca ttaggtgcca tgggcttacc
     1681 ggcacaaggt ttagcattaa ttattggtgt tgaccgtatc ttagatatgg tacgtacatg
     1741 tgtaaacgtt attggtaacg cattatcaac aatcgttata gctaaatggg aaaacgtata
     1801 tgacaaagca aaaggtcaag aatatttaaa atcaatttaa aaaatactat ctgacattta
     1861 aagcccctta caacctttgg ttgttagggg cttttatgca tgcgcaaaag cgcaggcgaa
     1921 ccgaagcgca gtgacctcag gttgttaggg gcttttatgc atgcgcaaaa gcgcaggcga
     1981 accgaaaata gactgacaca gtaattctta aaaagcagca gtaagataat tttcaattag
     2041 aaaacatctt actgctgctt ttaatatctt ctatattact caaaattaaa gcttaattag
     2101 tgtttcatgt gcattttgcc catgtcatga attttcatca ttaaaccgtg agggacgtca
     2161 tcatggtcaa cttttttaac tttaacaatt ttatctgaat ctgctaattt ataaacaatt
     2221 acaaaatcac cttttttaac tttaacatct gaatctgaag gtaatttaac atttttt
//
LOCUS       DAHHSC010000271         2227 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.275, whole genome shotgun sequence.
ACCESSION   DAHHSC010000271 DAHHSC010000000
VERSION     DAHHSC010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2227)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2227)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2227
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.275"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            78..1067
                     /gene="trpS"
                     /locus_tag="NBI57_002537"
     CDS             78..1067
                     /gene="trpS"
                     /locus_tag="NBI57_002537"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HCD1823458.1"
                     /translation="METLFSGIQPSGIPTIGNYIGALKQFVDVQNDYDCYFCIVDQHA
                     ITMPQDRLKLRKQTRQLAAIYLASGIDPDKATLFIQSEVPAHVQAGWMLTTIASVGEL
                     ERMTQYKDKAQKAVEGIPAGLLTYPPLMAADIVLYNTNIVPVGDDQKQHIELTRNLVD
                     RFNSRYNDVLVKPEIRMPKVGGRVMSLQDPTRKMSKSDDNAKNFISLLDEPNVAAKKI
                     KSAVTDSDGIIKFDRDNKPGITNLISIYAGLTDMPIKDIEAKYEGEGYGKFKGDLAEI
                     VKAFLVEFQEKYESFYNSDKLDDILDQGRDKAHKVSFKTVKKMEKAMGLGRKR"
     gene            complement(1109..1990)
                     /locus_tag="NBI57_002538"
     CDS             complement(1109..1990)
                     /locus_tag="NBI57_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCD1823459.1"
                     /translation="MQNKSKSPFKIALSRFIHNKIAMLSIIFLLIITIVSIIAPLIAP
                     FPVNQQDLLNIKGEMTAQNILGTDSGGRDNFSRLLYAGRISLSIGITSTIGMLLIGIT
                     VGVISGYFGGIVDTLLMRITEFVMLFPFLIFAIVLNAALGDKIKNPYGSAIILVLVII
                     VLSWGGIARLVRGKVLQEKENEYFLAAKSIGTPTYKIILKHLLPNILSVVIVQATLLF
                     AGMIVVESGLSFLGFGISKAIPSWGNMLSDAQEGDVISGKPWIWMPPAIMITLTILSI
                     NFVGEGLKDAFNPRGRR"
     gene            complement(2002..>2227)
                     /locus_tag="NBI57_002539"
     CDS             complement(2002..>2227)
                     /locus_tag="NBI57_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HCD1823460.1"
                     /translation="TFLGADIVSILGGAVITETIFSYNGIGKLFLESVIGQDYPLMMA
                     LTLFFSFLGLLGNLISDITYGFIDPRIRSN"
BASE COUNT          825 a          382 c          339 g          681 t
ORIGIN      
        1 ctaacacatc ttacattttt aagaaatact gttataatga taattattaa aatattacta
       61 agaaagtagg catttaaatg gagacattat tttcaggcat ccaacctagt ggaattccta
      121 ctattggaaa ttatattggc gcactaaaac aatttgttga tgtgcaaaat gactatgatt
      181 gttatttctg tatcgtagat caacatgcaa ttacaatgcc acaagatcgt ttaaaattac
      241 gtaaacagac cagacaatta gcagcgattt atttagcttc tggtatagat ccagacaaag
      301 caacattgtt catacaatct gaagtccctg cacacgtaca agcaggatgg atgttaacta
      361 cgattgcttc tgttggagaa ttagagcgta tgacgcaata caaagataaa gctcagaaag
      421 cagttgaagg tataccagct ggtctattaa catatccacc tttaatggca gctgatattg
      481 ttctttacaa tactaatatc gttccagttg gagatgacca aaagcagcat atcgaattga
      541 ctcgtaacct tgtagataga tttaatagtc gctataatga tgtgcttgtg aaacctgaaa
      601 ttcgtatgcc taaagttggt ggacgtgtca tgagtttaca agacccaaca agaaaaatga
      661 gtaagagtga tgataatgct aaaaacttca tttcattatt ggacgagccg aatgttgcag
      721 ctaaaaaaat taaaagcgca gtaactgatt cagatggtat tattaaattt gatcgtgaca
      781 acaagccagg tataacaaat ttaatttcaa tatacgctgg attaaccgac atgccaatta
      841 aagatattga ggcaaaatat gaaggggaag gttatggtaa atttaaaggt gaccttgctg
      901 aaatagttaa agcattttta gtagaattcc aagaaaaata cgaaagtttc tataactcag
      961 ataaacttga tgatatttta gatcaaggta gagataaagc acacaaagtt tcatttaaaa
     1021 ctgtcaaaaa aatggaaaaa gcgatgggat taggacgtaa gagataaaat aaagaggtac
     1081 tagaattaac tagtgcctct tttattattt aacgtctacc tctaggatta aaagcatctt
     1141 taagcccttc ccctacaaag tttatactta atatagttaa tgtaatcatt atagcaggtg
     1201 gcatccatat ccacggttta ccacttataa catccccttc ttgagcatca ctcaacatat
     1261 taccccaaga tggtattgct ttactaattc cgaatcctaa aaagctcaat cctgattcca
     1321 ctacaatcat accggcaaat aacaatgttg cttgtacgat aactacactt aatatattcg
     1381 gcaaaagatg tttcaaaata attttatatg tgggtgtacc aattgatttt gctgccaaaa
     1441 agtattcatt ttctttttct tgaagtactt taccacgaac aagtcttgca atacctcccc
     1501 aacttaatac gataataact agaacaagaa ttatggcaga tccataagga tttttaattt
     1561 tatctccaag tgcagcattt aatacaattg caaatattaa aaatggaaat aacataacaa
     1621 attcggttat tctcattaat aatgtatcaa caatacctcc aaaataacca gaaatcactc
     1681 caactgtaat tccaatcaaa agcattccta ttgtagatgt aattccaatg gataatgaaa
     1741 tacgacctgc atataacaaa cgactaaagt tatccctacc accagagtct gtaccaagaa
     1801 tgttttgtgc tgtcatttca ccttttatat ttaataaatc ttgttggttc actggaaaag
     1861 gagctattaa tggcgctata attgatacaa tagttatgat taataaaaaa ataatcgata
     1921 acattgcaat tttattatga ataaatctag acaatgcaat tttaaaaggc gattttgact
     1981 tattttgcat tccaatcccc cctagttact tctaattctt ggatctataa atccataagt
     2041 aatatcagaa atcaaattac ccaataaacc taaaaatgag aaaaataacg ttaatgccat
     2101 cattaatgga tagtcttgac ctattaccga ttctaaaaat aatttaccga taccgttata
     2161 tgaaaagata gtctcagtaa tcacagctcc acctaaaata cttacaatat cagcacctaa
     2221 aaatgta
//
LOCUS       DAHHSC010000272         2153 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.276, whole genome shotgun sequence.
ACCESSION   DAHHSC010000272 DAHHSC010000000
VERSION     DAHHSC010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2153)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2153)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2153
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.276"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            complement(<1..586)
                     /locus_tag="NBI57_002540"
     CDS             complement(<1..586)
                     /locus_tag="NBI57_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1823461.1"
                     /translation="MQHIIKTALQQTFNYKTNKSIYNILVGKKSHQTFFDACSQQQLS
                     LYHSLPLLKYPSFELFLEKINEFNAEMEIMLHPRYTFESMGQTFQAIQLLVQTMSNTK
                     QHVFHFVPISQNNKIQETVKIVYNYIKENQLQIDFENELHNLFKAITLKGPCYLHYYL
                     QGYDEPMYTRQQVSLIEKLSQQQLFEYEMNNLVTM"
     gene            694..942
                     /locus_tag="NBI57_002541"
     CDS             694..942
                     /locus_tag="NBI57_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002449743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HCD1823462.1"
                     /translation="MAKYTIVDMDTCIACGACGAAAPDIYDYDDEGIAFVILDDNQGT
                     AEVPEELYEDMEDAIDGCPTDSIKIADESFDGDALKFE"
     gene            complement(1048..1593)
                     /locus_tag="NBI57_002542"
     CDS             complement(1048..1593)
                     /locus_tag="NBI57_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HCD1823463.1"
                     /translation="MQQNKRLITISMLSAIAFVLTFIKFPIPFLPPYLTLDFSDVPSL
                     LATFTFGPVAGIVVALVKNLLNYLFSMGDPVGPFANFLAGASFLLTAYAIYKNKRSTK
                     SLITGLIIATIVMTIVLSILNYFVLLPLYGMIFNLADIANNLKVIIVSGIIPFNIIKG
                     IVISIVFILLYRRLANFLKRI"
     regulatory      complement(1655..1789)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(1995..>2153)
                     /locus_tag="NBI57_002543"
     CDS             complement(1995..>2153)
                     /locus_tag="NBI57_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000913244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1672 family protein"
                     /protein_id="HCD1823464.1"
                     /translation="SEVLKKKKNIPHDLNVSLQISNKYINTKRPNYSKKEVIEVGVFN
                     HEKANTND"
BASE COUNT          699 a          319 c          356 g          779 t
ORIGIN      
        1 tcattgtcac taaattattc atttcgtatt caaacaattg ctgttgagat agtttttcta
       61 ttaaactaac ttgctgtctc gtatacattg gttcatcata gccttgcaaa taataatgta
      121 agtaacatgg cccttttaaa gttattgctt taaacaagtt atgtagttca ttttcaaagt
      181 caatttgtaa ttggttttct ttaatgtagt tatagacaat ctttacagtt tcttgtatct
      241 tattattttg agagattggt acaaaatgaa aaacatgttg tttggtatta gacatggttt
      301 gcactaatag ttgaattgct tgaaatgttt gccccatgct ttcaaatgta tatctaggat
      361 gcaacatgat ttccatttca gcattaaatt cattgatttt ttctaaaaat agctcaaaag
      421 acggatattt taatagtggt aaactgtgat ataatgacaa ctgttgttga ctacaagcgt
      481 caaaaaaggt ttggtgagat ttcttaccaa ctaagatatt gtaaatactt ttatttgttt
      541 tatagttaaa tgtttgttgt aatgctgttt ttataatgtg ttgcaaattt aaccctcact
      601 ctgtaaaacg gataatttga aaataaaaat gatgctgtct aaaattttag tcacattact
      661 attttagcat ttttatcaaa ggaggcgaag acattggcaa aatatacaat cgttgatatg
      721 gatacttgta ttgcatgtgg tgcatgcggt gcagcagcgc cagatatata tgattacgac
      781 gacgaaggta ttgctttcgt aatccttgac gataaccaag gtactgcaga agtacctgag
      841 gaattatatg aagatatgga agatgcaatt gatggatgcc ctacagattc tattaaaatt
      901 gcagacgaat catttgatgg ggacgcttta aaatttgaat aattgatttt ttagacaaat
      961 gttctataga aaaagcaggc aagctatcgt ttattgatag tcacctgctt tttattatta
     1021 tgtgtatatt attttgcttt aatttgatta aattcttttc aagaaattcg caagccttct
     1081 atatagtaaa ataaatacaa tagaaatgac gataccttta ataatattga atggtataat
     1141 tcctgataca atgattactt taagattatt tgcgatatca gctaagttaa atatcatacc
     1201 gtacaaaggt aatagaacga aatagttcaa aatactcaac acgatagtca taacgattgt
     1261 tgcaatgatt aatccagtaa tcaaagattt tgttgaacgt ttatttttat agatggcgta
     1321 agcagttaat aagaaacttg cgcctgctaa aaagttagca aacggcccaa ctggatcgcc
     1381 catactaaat aagtagttca gcaagttttt aaccagtgca actacgatac cggcaactgg
     1441 tccaaacgta aatgtagcta gtagtgacgg tacatcacta aaatctaaag ttaagtatgg
     1501 tggcaaaaat ggtataggaa acttgataaa agttaacaca aacgcaatcg cgctcaacat
     1561 acttattgtg ataagacgtt tattttgttg catattcaat tttctccttc cattcatctc
     1621 gtaagaatag gaggattatt gcacaacaaa aaacctccat cttctcccat ccagactata
     1681 ctgtcggctc tagaatctca ctagatcagc cactaatatg atacatacta gcaggtcgca
     1741 ggctttattt actgccggtt gggaattaca cccgaccccg aagatgaatt ttacattatt
     1801 aaatttaatt ctcattaatt ttatactata tatcacaaaa cctcaataac tatgctttaa
     1861 ttagatcata gttacattat tggagagtga tacattggtg atttgagttc attggttgga
     1921 taggtttgat tggtttgtgg ttttaactgt tgttgagatg gcttttttga acctaccttt
     1981 ttattgttat tagtttaatc attcgtgtta gctttttcat gattaaatac acctacttct
     2041 attacttcct tcttactata gttaggtctt tttgtattta tatatttatt agaaatttga
     2101 agagaaacat tcaaatcatg aggaatattc tttttctttt ttagtacttc aga
//
LOCUS       DAHHSC010000273         2150 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.277, whole genome shotgun sequence.
ACCESSION   DAHHSC010000273 DAHHSC010000000
VERSION     DAHHSC010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2150)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2150)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2150
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.277"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            <1..659
                     /locus_tag="NBI57_002544"
     CDS             <1..659
                     /locus_tag="NBI57_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1823465.1"
                     /translation="GVSEEMMKHLKVVKQNIERIKNQNDIYGRQIAEIRSIMKQQDAT
                     ILDGNFQINCSGENMVQGLVIPSNYLGRKMKILKDHIDDGIKKIADYVQSIYDEYASK
                     IVDVIKYLINTIPKIRKNLRHAIEMLNVKKKEFLSLIPNVTCNYIKTKLEELDNTLGK
                     WEPFLNDLKAVSPILDNHLDDIVKNMKPLIVQMLFEPSHYDDMFISRKALTPVFSSVL
                     "
     gene            complement(702..1907)
                     /locus_tag="NBI57_002545"
     CDS             complement(702..1907)
                     /locus_tag="NBI57_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCD1823466.1"
                     /translation="MWIEKFKNKNNETKYRYYEKYKDPYTDKWKRVSVVLNKNTKQSQ
                     KEAMFRLEEKIKEKLNNKSSSELKTLTFHALLDEWLEYHIKTSGSKLTTLNNIKIRIK
                     NIKRYCSENLLLNKLDTKYMQIFINKLSDIYSQNQVTRQLGDMKGAIKYAVKFYNYPN
                     EYLLTNVKIPKRRKTIEDIEKDESKMYNYLEMNQVLQIRDHILNDNKLHKRNRILIAS
                     ILKVQALTGMRIGELQALQEKDIDLLNKTINITGTIHRIKYEEGFGYKDTTKTISSKR
                     SISINSRTVEIFKKIILENKMLKRWNSSYVDRGFIFTTKKGNPLCNNQIAGVLKKTTK
                     ALNMNKKVTTHTFRHTHITLLVEMNVSLKAIMKRVGHVDEKTTIRIYTHVTEKMDREL
                     TQKLENIPS"
     gene            2033..>2150
                     /locus_tag="NBI57_002546"
     CDS             2033..>2150
                     /locus_tag="NBI57_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HCD1823467.1"
                     /translation="MTQFLGALLLTGVLGYIPYKYLTMIGLVSEKNKVINTPV"
BASE COUNT          690 a          287 c          339 g          834 t
ORIGIN      
        1 atggagtatc tgaagaaatg atgaaacatt tgaaagtagt gaaacagaat atagagcgaa
       61 taaaaaatca aaatgatatt tatggtaggc aaattgcaga aattagaagt attatgaaac
      121 aacaagatgc aacaatttta gatgggaatt tccaaattaa ttgtagcggc gaaaatatgg
      181 tacagggtct agttatacct tctaattatt taggaagaaa aatgaaaata ttaaaagacc
      241 atatcgatga tggtattaaa aaaatagcag actatgttca aagtatatat gatgaatatg
      301 catcgaaaat tgttgatgta ataaaatatt tgattaatac aattcccaaa atacgtaaga
      361 atttaagaca tgcaattgaa atgttaaatg taaaaaagaa agaatttttg tccctgattc
      421 ctaatgtaac ttgtaattat attaaaacta aattagaaga attagataat actttaggca
      481 aatgggagcc atttcttaat gatttaaaag cagtgtcacc aattttagat aaccatttag
      541 atgatattgt taagaacatg aagcctttga ttgtacagat gttatttgaa ccatcacatt
      601 atgatgatat gtttatttca agaaaagctt taacgccagt gttctcaagc gttttataaa
      661 gcttgtaaaa aatataaggg caaaaaaagg gcggatttaa gctaacttgg aatgttttcg
      721 agtttttgag ttagttctct atccattttt tcagttacat gagtatatat gcgaatggtt
      781 gttttttcat ctacatgtcc tacccttttc ataattgctt ttaaagaaac attcatttct
      841 actaataaag ttatgtgtgt atgtctaaat gtgtgcgtgg taactttctt attcatattt
      901 aaagcttttg tagttttctt aagcacaccg gcgatttgat tattacataa aggattccct
      961 ttttttgttg tgaatatgaa ccctctgtca acatagctcg aattccatct tttcaacatt
     1021 ttgttttcca gtattatctt tttaaaaatt tctacggttc tagaattgat gctgatactt
     1081 ctttttgaac ttatagtctt tgtagtgtct ttgtatccga atccttcctc gtatttaatg
     1141 cggtgaattg tacctgttat attgatagtt ttgtttaata aatctatatc tttttcctgc
     1201 agtgcttgta gttctcctat gcgcatacca gttaaagcct gtacttttaa gatgctggca
     1261 attaaaatgc gatttcgctt gtgtaactta ttatcattta gtatatgatc acgtatctgt
     1321 aggacttggt tcatttctaa atagttgtac attttagatt catctttttc gatatcctct
     1381 attgtttttc ttcttttagg aattttgaca ttagttaaca aatattcatt tggataattg
     1441 taaaatttaa ctgcatattt aatagctcct ttcatatctc cgagttgacg ggttacttga
     1501 ttttgagaat agatatctga taatttatta ataaatatct gcatatattt tgtatcaagt
     1561 ttgtttaaaa gcaagttctc agagcagtat cgtttaatgt ttttaattct tatttttata
     1621 ttattaagag tagtcaactt tgaacctgat gtttttatat gatattcaag ccattcatct
     1681 aatagcgcgt gaaaagtcaa agtttttaat tcgcttgacg acttgttgtt cagtttttct
     1741 tttatttttt cttctaaacg aaacattgct tctttttgag attgttttgt attcttgttc
     1801 aacacaacac ttacgcgctt ccatttatct gtgtatggat ctttatactt ctcgtagtat
     1861 ctgtatttag tttcgttatt tttgttttta aatttttcaa tccacatgtt tatacctcct
     1921 gcaagaacgt atgttctata aaatattaaa aaataataag ggtagtcggg ctacccgtaa
     1981 tttagtacta ggtactaaat gtgttataat aaaataaaaa gtaggtgata agatgactca
     2041 atttctaggg gcgcttcttc ttacaggagt tttaggttac ataccatata aatatctaac
     2101 aatgataggt ttagttagtg aaaaaaacaa ggttatcaat actcctgtat
//
LOCUS       DAHHSC010000274         2142 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.278, whole genome shotgun sequence.
ACCESSION   DAHHSC010000274 DAHHSC010000000
VERSION     DAHHSC010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2142)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2142)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2142
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.278"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            <1..121
                     /locus_tag="NBI57_002547"
     CDS             <1..121
                     /locus_tag="NBI57_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1823468.1"
                     /translation="NDMMNLVINQIVIKLNYRDDWNEFIDIYSNTILLGLTIN"
     gene            150..368
                     /locus_tag="NBI57_002548"
     CDS             150..368
                     /locus_tag="NBI57_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-dependent RNA polymerase auxiliary subunit
                     epsilon family protein"
                     /protein_id="HCD1823469.1"
                     /translation="MAVFKVFYQHNRDEVIVRENTQSLYVEAQTEEQVRRYLKDRNFN
                     IEFITKLEGAHLDYEKENSEHFNVEIAK"
     gene            368..2065
                     /locus_tag="NBI57_002549"
     CDS             368..2065
                     /locus_tag="NBI57_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HCD1823470.1"
                     /translation="MKQLHPNEVGVYALGGLGEIGKNTYAVEYKDEIVIIDAGIKFPD
                     DNLLGIDYVIPDYTYLVQNQDKIVGLFITHGHEDHIGGVPFLLKQLNIPIYGGPLALG
                     LIRNKLEEHHLLRTAKLNEINEDSVIKSKHFTISFYLTTHSIPETYGVIVDTPEGKVV
                     HTGDFKFDFTPVGKPANIAKMAQLGEEGVLCLLSDSTNSLVPDFTLSEREVGQNVDKI
                     FRNCKGRIIFATFASNIYRVQQAVEAAIKNNRKIVTFGRSMENNIKIGMELGYIKAPP
                     ETFIEPNKINTVPKHELLILCTGSQGEPMAALSRIANGTHKQIKIIPEDTVVFSSSPI
                     PGNTKSINRTINSLYKAGADVIHSKISNIHTSGHGSQGDQQLMLRLIKPKYFLPIHGE
                     YRMLKAHGETGVECGVEEDNVFIFDIGDVLALTHDSARKAGRIPSGNVLVDGSGIGDI
                     GNVVIRDRKLLSEEGLVIVVVSIDFNTNKLLSGPDIISRGFVYMRESGQLIYDAQRKI
                     KTDVISKLNQNKDIQWHQIKSSIIETLQPYLFEKTARKPMILPVIMKVNEQKESNNK"
BASE COUNT          757 a          336 c          370 g          679 t
ORIGIN      
        1 gaacgacatg atgaacctag ttattaacca aatcgttatt aagttaaatt atagagatga
       61 ttggaatgaa tttatcgata tatactccaa tacgatttta ctagggttaa caataaatta
      121 aacaaacatt cttaggagga atttttaaca tggcagtatt taaagttttt tatcaacata
      181 acagagacga ggtaattgtg cgtgaaaata cacaatcact ttatgttgaa gctcaaacag
      241 aagaacaagt acgtcgttac ttgaaagatc gtaattttaa tatcgaattt atcactaaat
      301 tagagggcgc acatttagat tacgaaaaag aaaactcaga acactttaat gtggagattg
      361 ctaaataatg aaacaattac atccaaatga agtaggtgta tatgcacttg gaggtctagg
      421 tgaaatcggt aaaaatactt atgcagttga gtataaagac gaaattgtca ttatcgatgc
      481 aggtatcaaa ttccctgatg ataacttatt agggattgat tatgttatac ctgactacac
      541 atatctagtt caaaaccaag ataaaattgt tggcctattt ataacacatg gtcacgaaga
      601 ccatataggc ggtgtgccct tcctattaaa acaacttaat atacctattt atggtggtcc
      661 tttagcatta ggtttaatcc gtaataaact tgaagaacat catttattac gtactgctaa
      721 actaaatgaa atcaatgagg acagtgtgat taaatctaag cactttacga tttctttcta
      781 cttaactacg catagtattc ctgaaactta tggcgtcatc gtagatacac ctgaaggaaa
      841 agtagttcat accggtgact ttaaatttga ttttacacct gtaggcaaac cagcaaacat
      901 tgctaaaatg gctcaattag gcgaagaagg cgttctatgt ttactttcag actcaacaaa
      961 ttcacttgtg cctgatttta ctttaagcga acgtgaagtt ggtcaaaacg tagataagat
     1021 cttccgtaat tgtaaaggtc gtattatatt tgctaccttc gcttctaata tttaccgagt
     1081 tcaacaagca gttgaagctg ctatcaaaaa taaccgtaaa attgttacgt tcggtcgttc
     1141 gatggaaaac aatattaaaa tcggtatgga acttggttat attaaagcac cacctgaaac
     1201 atttattgaa cctaataaaa ttaataccgt accgaagcat gagttattga tactatgtac
     1261 tggttcacaa ggtgaaccaa tggcagcatt atctagaatt gctaatggta ctcataagca
     1321 aattaaaatt atacctgaag ataccgttgt atttagttca tcacctatcc caggtaatac
     1381 aaaaagtatt aacagaacta ttaattcctt gtataaagct ggtgcagatg ttatccatag
     1441 caagatttct aacatccata cttcagggca tggttctcaa ggtgatcaac aattaatgct
     1501 tcgattaatc aagccgaaat atttcttacc tattcatggt gaataccgta tgttaaaagc
     1561 acatggtgag actggtgttg aatgcggcgt tgaagaagat aatgtcttca tctttgatat
     1621 tggagatgtc ttagctttaa cacacgattc agcacgtaaa gctggtcgca ttccatctgg
     1681 taatgtactt gttgatggta gtggtatcgg tgatatcggt aatgttgtaa taagagaccg
     1741 taagctatta tctgaagaag gtttagttat cgttgttgtt agtattgatt ttaatacaaa
     1801 taaattactt tctggtccag acattatttc tcgaggattt gtatatatga gggaatcagg
     1861 tcaattaatt tatgatgcac aacgcaaaat caaaactgat gttattagta agttaaatca
     1921 aaataaagat attcaatggc atcagattaa atcttctatc attgaaacat tacaacctta
     1981 tttatttgaa aaaacagcta gaaaaccaat gattttacca gtcattatga aggtaaacga
     2041 acaaaaagaa tcaaacaata aataatcaaa aagctactaa ctttgaagtg aagttttaat
     2101 taaactcacc acccattgtt agtagctttt tctttatata tg
//
LOCUS       DAHHSC010000275         2130 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.279, whole genome shotgun sequence.
ACCESSION   DAHHSC010000275 DAHHSC010000000
VERSION     DAHHSC010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2130)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2130)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2130
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.279"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            121..1161
                     /locus_tag="NBI57_002550"
     CDS             121..1161
                     /locus_tag="NBI57_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="linear amide C-N hydrolase"
                     /protein_id="HCD1823471.1"
                     /translation="MQQVISDIMTFRGSHFELGVKTGKWLQQTPLLKNREKEWKKRVP
                     RFDIDVNETYQIFQTYAPQIWEELMGLQSILKMPTRQIILNFGHYRFTDLKESGCTVF
                     QGKDFMVRNYDYHPATYDGRYLLYQPTDSGLAQIGPVSRVTGRMDGMNESGLTMGYNF
                     MHRKKPANGFVCYMIGRLILENCRNVTEAIQLLKEIPHRSSFSYILMDKSLNHAIVEV
                     TPRSIDVRYDNICTNHFEILTHENRNYTKESKERLARTISQTNDNLDMTTAFKLFNNP
                     QYEIYSKLFKSWSGTIHTSMYQPETLTAYFTLGENAPPEIIDFKSWLDGQDLNITHFT
                     GNIDTDLTFANK"
     gene            complement(1218..2060)
                     /locus_tag="NBI57_002551"
     CDS             complement(1218..2060)
                     /locus_tag="NBI57_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat protein"
                     /protein_id="HCD1823472.1"
                     /translation="MKKFFFIGLLVFVVFFTAATIIWFSYDKNKYGTKQYDKTFKDDA
                     FDNVSINLDSTELRIKRGNQFRVKYDGDNDILINIVDKTLKISDKRSKTRGYAIDMNP
                     FHENKKTLTIEMPDKMIKRLNLSSGAGSVRISDVDLENTSIQSINGEVVIKNSNLDAL
                     DSKTNNSSTYISKSNIKNSNIKVVIGTLQIDKSQIKQSIFLNDHGDIEFKNMPSKVDA
                     KASTKQGDIRFKYDSKPEDTILKLNPGTGDSVVKNKTFTNGKVGKSDNVLEFYTIDGN
                     IKVE"
BASE COUNT          741 a          365 c          295 g          729 t
ORIGIN      
        1 cctcaaacaa ttttatattt catttagtaa aatataactt tatttaacat gacattcaat
       61 atacatacga ttactattta tttacaatat attacaacta aaaagaaagg ggccgatgat
      121 atgcaacaag tgatatcaga tattatgact tttagaggtt cacatttcga gttaggtgtt
      181 aaaactggta aatggcttca acaaacacct cttctaaaaa atcgagaaaa agaatggaaa
      241 aaacgtgtcc cacgtttcga tatagatgta aatgaaactt atcaaatatt ccaaacttac
      301 gcacctcaaa tatgggaaga attaatggga ctacaaagta tactcaaaat gccaacacgc
      361 caaattattt taaactttgg acattatcgt tttaccgatt taaaagaaag tggttgcacg
      421 gtctttcaag gcaaagactt catggttaga aattatgatt accaccctgc aacttatgat
      481 ggtcgctact tactatatca acctacagat agcggtttag ctcaaattgg tcctgtatca
      541 agggtaactg gaagaatgga tggtatgaat gagtcaggat taactatggg ctataacttt
      601 atgcaccgga agaaaccagc aaatggtttt gtatgttata tgattggtcg tctaattctt
      661 gaaaattgcc gaaatgtaac agaagcaata caattattga aagaaatacc acacagaagc
      721 tcatttagct acatactgat ggacaaatca ttgaaccatg caattgttga agtgacaccg
      781 agatcaatag atgttcgtta tgataatata tgtacaaatc attttgaaat attaacacat
      841 gaaaatcgta attatacaaa agaatctaaa gaaagactgg cgcgtaccat ttcacaaact
      901 aatgataatt tagatatgac tacagcattt aagttattca ataatcctca gtatgaaatt
      961 tacagtaaac tattcaaaag ctggtctggc accatccata catcaatgta tcaacctgaa
     1021 acattaactg catattttac attgggagaa aatgcgccac cagaaataat agatttcaaa
     1081 tcatggttag atggtcaaga ccttaatatc actcacttta ctggcaatat agataccgac
     1141 ttaacttttg ccaacaaata aataacaccg atattagaga aattatgctt cctaatatcg
     1201 gtgcttacat cctttattta ttcaactttg atattaccat caatcgtata aaattctaaa
     1261 acattgtcgc ttttcccaac tttaccatta gtaaatgttt tatttttaac tacgctatca
     1321 cccgttcccg gatttagctt tagtatagtg tcttcaggtt tactatcata cttaaaacga
     1381 atatctcctt gtttagtaga agcttttgca tctacttttg atggcatgtt tttaaattca
     1441 atgtcaccat gatcgtttaa aaatatggat tgtttaattt gactcttgtc gatttgtagc
     1501 gtaccaataa caactttaat attgctattt ttaatgttac ttttactaat ataagtagaa
     1561 ctattatttg tctttgaatc tagtgcgtca agatttgagt ttttaataac tacttcaccg
     1621 ttaatgcttt gaatacttgt gttctctaaa tcaacatcac tgattctaac acttcctgct
     1681 ccagatgata gatttaaacg tttaatcatt ttatcaggca tttcaatcgt taacgttttc
     1741 ttattctcat gaaaaggatt catatcaatt gcatatcctc ttgtcttaga ccttttatca
     1801 ctaatcttca acgtcttatc tactatatta attaatatat cattgtcacc atcatattta
     1861 actctaaatt gattcccccg ttttatacga agttctgtac tatccaaatt tatagataca
     1921 ttgtcaaaag catcgtcttt gaatgtttta tcatattgtt tagtaccata tttgttttta
     1981 tcatagctga accaaataat ggttgctgct gtaaaaaaga caacaaacac taaaagccca
     2041 ataaaaaaga atttcttcat tttctaacgc ttcctttgac agttttaata taccaaagta
     2101 aatattttaa gattaaacga taaatgtatt
//
LOCUS       DAHHSC010000276         2111 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.280, whole genome shotgun sequence.
ACCESSION   DAHHSC010000276 DAHHSC010000000
VERSION     DAHHSC010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2111)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2111)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2111
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.280"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            223..2037
                     /gene="pepF"
                     /locus_tag="NBI57_002552"
     CDS             223..2037
                     /gene="pepF"
                     /locus_tag="NBI57_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HCD1823473.1"
                     /translation="MSQGLPLREDVPVSETWDLVDLFKDDQQYYESIDALVQQANQFH
                     HTYATTLNSIEQINTALAELENILIALDRLSNYAELRLSVDTSNIEAQVLSAKLSTTY
                     GKIVSQLSFVESEILELPEEILQQLEESCPYQHYIKQLIKQKPFQLSASVEQVLATLS
                     PTLNSPYDLYGTTKMLDITFDSFEHDGTTYPVDYATFENDYEDNKDPEFRRKSFKSFS
                     DGIRKYQHTTAATYNMQVQQEKIEADLRGFESVIDYLLHSQEVTRDMFDRQIDMIMRD
                     LAPVMQKYAKLLQRIHGLDNMRFEDLKISVDPDYEPEISIEDSKNYIFGALSVLGDDY
                     TNMLREAYDQRWIDFAQNKGKDTGAFCASPYFTHSYVFISWTGKMAEAFVLAHELGHA
                     GHFTLAQKHQPYLESEASMYFVEAPSTMNEMLMANYLFNTSDNPRFKRWVIGSILSRT
                     YYHNMVTHLLEAAYQREVYHKVDQGESLNAPTLNEIMLNVYKQFFGDAVDMTEGAELT
                     WMRQPHYYMGLYSYTYSAGLTIGTVVSQKIKNEGQPAVDAWLETLKKGGSVSPVELAN
                     IAGVDITTEQPLKSTIQYISDLVDEVEKLTDEIEQANN"
BASE COUNT          752 a          330 c          356 g          673 t
ORIGIN      
        1 catatcgaat aatgtcatat tcattctcct tttatttcat tactttcctt gtatacattt
       61 tattataaat ttttaaaaac ttaaacaata gcagtcagtt tcaagcaata ttctatctac
      121 tattagaaaa atcattgttc cttgcgacat ggaaatcgta acattatcgt ttaggagaca
      181 aaattatgta taatgaatgt attataccaa aggagtgatt atatgtctca aggtttacct
      241 ttaagagaag atgttcctgt ttcagaaaca tgggatttag tagacttatt taaagatgat
      301 caacaatatt atgaaagtat tgacgctcta gtacaacaag caaatcaatt tcatcataca
      361 tatgcaacaa cattaaattc aatcgaacaa attaatactg ctttagctga attagaaaat
      421 attttaattg ccttagatcg cttaagtaat tatgcagaac tacgtttaag tgtagatact
      481 agtaatatcg aggcacaagt attgagcgct aaattatcta ctacatacgg taaaattgtt
      541 agccaattat catttgtaga gtcagaaata cttgaattac cagaagaaat acttcaacaa
      601 ttagaagaat catgtccata tcaacactat attaaacagt taataaaaca aaagccattc
      661 caattatctg cgtcggtaga acaagtatta gcaactttat cacctacgct aaacagtcct
      721 tacgatttat acggcacgac aaaaatgcta gatattacat tcgattcatt tgaacatgat
      781 ggtacaacgt accctgtcga ctatgctacg tttgaaaatg attatgaaga taataaagat
      841 cctgagttta gacgtaaaag tttcaaatcg tttagcgatg ggattcgaaa atatcagcat
      901 actaccgcgg ctacatataa tatgcaagta caacaagaaa aaattgaagc tgatttacgt
      961 ggatttgaat cagtcatcga ttatttatta catagtcaag aagtaacgcg tgatatgttt
     1021 gaccgtcaaa tcgatatgat tatgcgtgac ttggcaccag ttatgcagaa atatgctaaa
     1081 cttttacaac gtattcacgg attagataac atgcgttttg aagacttgaa gatttctgta
     1141 gaccctgatt atgaaccaga gatttcaatt gaagactcaa aaaattatat tttcggtgcg
     1201 ttaagtgttt taggtgatga ctatacaaac atgttacgtg aagcatacga tcagcgatgg
     1261 attgattttg cacaaaataa aggtaaagat acaggcgcat tttgtgcaag tccatacttt
     1321 acacattcat atgtgtttat ttcttggact ggtaaaatgg ctgaagcatt tgtcttagca
     1381 catgaattag gtcatgcagg tcattttaca ttagctcaaa aacatcaacc atatcttgaa
     1441 tcagaagcat caatgtactt tgttgaagcc ccttctacaa tgaatgaaat gttgatggcc
     1501 aattatttat ttaacacaag tgataatcca agatttaagc gttgggttat tggctcaatt
     1561 ttatctagaa catattatca taatatggtt acccatttat tagaagctgc ttatcaacgt
     1621 gaagtgtatc acaaagtaga tcaaggtgaa tctttaaatg cgccgacatt aaatgaaata
     1681 atgctaaatg tttataaaca attttttgga gatgcagtag acatgactga gggtgctgaa
     1741 ttaacatgga tgcgtcaacc tcattactat atgggattat attcgtatac gtattctgct
     1801 ggcttaacaa tcggaactgt cgtttctcaa aagattaaaa atgaaggcca accagctgtt
     1861 gatgcttggt tagaaacatt gaaaaaaggt ggtagtgtat cacctgtcga acttgcaaac
     1921 attgcaggtg tagacattac tacagaacag ccacttaaat ctacaattca atatatttct
     1981 gatttagtcg atgaagttga aaaattaaca gatgaaattg agcaagcaaa taactaagat
     2041 gcgagacaat ttatagtaga acaatataaa ccaagatttt cacattacta attgtgagaa
     2101 tcttggtttt t
//
LOCUS       DAHHSC010000277         2095 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.281, whole genome shotgun sequence.
ACCESSION   DAHHSC010000277 DAHHSC010000000
VERSION     DAHHSC010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2095)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2095)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2095
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.281"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            complement(96..929)
                     /locus_tag="NBI57_002553"
     CDS             complement(96..929)
                     /locus_tag="NBI57_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HCD1823474.1"
                     /translation="MEVKTFYNGNTMPQIGLGTFRVENDENCMESVKYAIEQGYRSID
                     TAKVYGNEEQVGAGIRAGLESTGIAREDLFITSKLYFEDFGRENVAAAYEASLSRLGL
                     KYLDLYLVHWPGTNEAVMVDTWKGMEDLYKNNKAKNIGVSNFEPEHLEALLAQVSIKP
                     VINQVEYHPYLTQHKLKLYLAAQHIVMESWSPLMNAQILNDETIKDIAQELGKSPAQV
                     VLRWNVQHGVVIIPKSVTPNRISENFQIFDFELSDEQMTRIDGLNQDKRIGPDPKTFE
                     G"
     gene            complement(1141..2049)
                     /locus_tag="NBI57_002554"
     CDS             complement(1141..2049)
                     /locus_tag="NBI57_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NERD domain-containing protein"
                     /protein_id="HCD1823475.1"
                     /translation="MDLSSPVVIGLIIAVVIALIFFVLFLVALGSKKKVKRQTEEKYE
                     QQEQNIKKSHEEALEKERIQNKKTITKQQEDYNHMVSTKDREIDALKLFSKNHSEYVT
                     DMRLIGIRERLVKEKRIRPEDMHIMANIFLPKDGFNNIERISHLVLTRTGLYIIDSQL
                     LKGHVYNGISGGQFKDLPPMEQVFDTLDLDKSRPQTIVMDQNDDKRSLSFVNYSDQIE
                     AIKQLAEDLQKELGAKYTPTSILYFNPKNEGDVTISNYNQNSAVKVLVGAEQLDEFFN
                     KFVFHGRIQYNVEDLQQMMDKIESFN"
BASE COUNT          663 a          398 c          268 g          766 t
ORIGIN      
        1 attaatagaa atcaactttt ttacattgcc taagaactta atgtcccaag ccctgacaat
       61 tcatctaacc ttttcatcag ttgagtgatt ttaatctatc cttcaaaagt ttttggatca
      121 ggtccaattc tcttatcttg atttaaacca tcaattcgcg tcatttgttc atctgataat
      181 tcgaaatcaa atatttggaa gttttcagag attctgtttg gtgttaccga tttagggatt
      241 ataaccacac catgctgcac attccatctt aaaacaactt gggcaggtga ctttcctaat
      301 tcttgagcaa tgtctttaat tgtctcatca tttaaaattt gtgcattcat caatggtgac
      361 caagattcca tcacgatatg ttgtgctgcc aaatataatt tcaatttatg ttgcgttaaa
      421 tatggatgat attcaacttg attaattaca ggtttaattg acacttgtgc caacaaagct
      481 tccaaatgtt caggttcaaa attgctgaca cctatatttt tagctttatt atttttatat
      541 aaatcttcca tacctttcca tgtatcaacc attacggctt cgttcgtgcc tggccaatgt
      601 actagataca aatctaagta ttttaaacct aatctagata aactagcttc gtaagcagct
      661 gctacatttt cacgaccgaa atcctcaaaa tataattttg aagtaataaa taagtcttct
      721 ctagcaatac cagttgactc caatccggca cgaatgccag cacctacttg ttcttcattc
      781 ccataaactt ttgcggtatc aatactacga tatccttgtt caatggcata cttaacactt
      841 tccatgcaat tttcatcatt ttccacacga aatgtcccta aaccaatttg tggcatcgtg
      901 tttccattat aaaatgtttt aacctccata aatatcgcct cacctttttg atgtattata
      961 ccctgttatc ataacaaatc tgagttgaat acatgagaaa aaacacttag agcaatcaac
     1021 cactaaaatt ctagtaatat ctctcaaaca ttaatcaaat tgtaaaagca attatgttta
     1081 atttatgaca aactaaaaaa gccgaagtca caacatatag tcatcacttc agcctaacat
     1141 ttaattgaat gattcaattt tatccatcat ttgttgtaag tcttccacgt tgtattgaat
     1201 acgaccatgg aatacaaatt tgttaaagaa ctcgtctaat tgttcagcac cgacaagcac
     1261 tttgacagca ctattttgat tataatttga aatcgttaca tcgccttcat ttttaggatt
     1321 aaagtataaa attgaagttg gcgtatattt ggcacctaat tctttttgta agtcttcagc
     1381 caattgttta atcgcctcaa tttgatctga ataatttaca aaagataatg aacgtttgtc
     1441 atcattttga tccatcacaa tagtttgtgg tctagattta tctaaatcca atgtatcaaa
     1501 tacttgttcc attggtggta aatctttaaa ttgaccgcca ctaataccat tataaacatg
     1561 accttttaac aattgagaat caataatata aagaccagtt cttgttaata ctaaatgact
     1621 aattcgttca atattattaa agccatcctt tggtaaaaag atatttgcca taatgtgcat
     1681 atcttctggt cgaattcgtt tttctttaac taatctttca cgaataccaa ttaatctcat
     1741 gtccgttaca tattcactat gatttttcga gaacaatttt aatgcgtcaa tctcacgatc
     1801 ttttgtacta accatgtgat tataatcttc ttgttgtttt gtaattgtct ttttattttg
     1861 aatacgctct ttctctaaag cttcttcatg agacttttta atgttttgtt cttgttgttc
     1921 atacttttct tctgtttgtc gcttaacttt tttcttacta cctaaggcaa ctaagaaaag
     1981 gacaaaaaag attaatgcaa tgactactgc aataatgagt ccaatgacta ccggtgaaga
     2041 taaatccatc acaacaacgc tcctttttaa tatatgaata actttaatta taata
//
LOCUS       DAHHSC010000278         2095 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.282, whole genome shotgun sequence.
ACCESSION   DAHHSC010000278 DAHHSC010000000
VERSION     DAHHSC010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2095)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2095)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2095
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.282"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            complement(210..731)
                     /locus_tag="NBI57_002555"
     CDS             complement(210..731)
                     /locus_tag="NBI57_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001627793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1823476.1"
                     /translation="MKRKVLVLTMGVICATQLWHSNHANALVTESVETNFVVKDSDNK
                     NILQTHTEITTEEKFSIVEKSQLNKLKALSNDNYIEYDLHTNQTGIKKGWLYGYSEID
                     SSHFTDRDKRVIRRDHVKEAQSLINDYKDTQSYEDLAKATAKVSTLSQSHQNYLNKQI
                     DKVNNKIEKTEKR"
     gene            complement(1066..2088)
                     /gene="emp"
                     /locus_tag="NBI57_002556"
     CDS             complement(1066..2088)
                     /gene="emp"
                     /locus_tag="NBI57_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular matrix protein-binding adhesin
                     Emp"
                     /protein_id="HCD1823477.1"
                     /translation="MKKKLLVLTMSTLFATQLINSNHAKASVTESVDKKFVVPESGIN
                     KIIPAYDEFKNSPKVNVSNLTDNKNFVVSEDKLNKIVDSSAASKIVDKNFAVPESKLG
                     NIVPEYKEINNRVNVATNNPASQQVDKHFVAKGPEVNRFITQNKVNHHFITTQTHYKK
                     VITSYKSTHVHKHVNHAKDSINKHFIVKPSESPRYTHPSQSLIIKHHFAVPGYHAHKF
                     VTPGHASIKINHFCVVPQINSFKVIPPYGHNSHRMHVPSFQNNTTATHQNAKVNKAYD
                     YKYFYSYKVVKGVKKYFSFSQSNGYKIGKPSLNIKNVNYQYAVPSYSPTHYVPEFKGS
                     LPAPRV"
BASE COUNT          627 a          299 c          327 g          842 t
ORIGIN      
        1 ctatatttta ggtgtcagaa cttatatatt tagccttatt tgtaaaatta aaaagcctac
       61 aacatgaatc gttgcagact atcatatcga gacaaagagg tttattagcg tctctatatc
      121 tatgcggaat ttgaaaagat tatttattat atttacgggg ataaattgtc atttaaaaac
      181 gagatgtata acttataatt tactttggat tagcgttttt cagttttctc tattttatta
      241 ttcactttat caatttgttt atttaaataa ttttggtgag actgactaag tgtacttact
      301 tttgcagttg ccttagcgag atcttcatag ctttgtgtat ctttataatc gttaattaag
      361 ctttgtgctt ctttaacatg atcacgtcta ataacgcgtt tgtcacggtc tgtgaaatgt
      421 gatgagtcaa tttcactata accatataac caaccttttt taatacctgt ttgattagta
      481 tgtaaatcat attctatgta gttgtcatta gatagtgctt ttaatttatt taattgactc
      541 ttttctacta ttgaaaattt ctcctcagta gtaatttcag tatgggtttg taaaatattt
      601 ttattatccg aatcttttac aacaaaatta gtttcaacac tctctgtcac taatgcgttt
      661 gcgtgattag aatgccataa ttgagttgca caaattacac ccattgttag tactagtact
      721 ttccttttca tttatttatg ctcccttatt tgctttgatt gtaaaaccga ctttttcacc
      781 ctgttttctt cgccattgaa aattatatac caatatttta gaaacaataa ttaacttgcg
      841 atgaacaaac tattaacaat aatcttgagt cttatattta tcttaattaa taatatattt
      901 atttaagatt atgttacttc caactttcaa agtagaaaaa cggtataatt tgttgatggg
      961 tgtttattga taaactgcaa aaaatacagc ctacaaccac atagattgta gactatattt
     1021 aaaataatag gtatttatca tatctcgtaa acttagtgcc aatttttata ctcgtggtgc
     1081 tggtaagcta cccttaaatt caggaacgta gtgtgtaggg ctataacttg gaacagcata
     1141 ttgataattt acatttttga tatttaatga tggtttccca attttataac catttgattg
     1201 tgaaaatgag aaatatttct tcacaccttt aactacttta taagaataga agtatttata
     1261 gtcatatgct ttatttactt tagcattttg atgtgttgct gttgtgttat tttggaaact
     1321 tggtacatgc atacgatgtg aattgtgacc atatggtgga attaccttga aactatttat
     1381 ttgtggcaca acacaaaagt gattaatttt aatgctagca tgccctggtg taacaaattt
     1441 atgcgcgtga tatccaggaa ctgcaaaatg atgcttgata attaaagatt gagatggatg
     1501 tgtatatcta ggcgattctg atggtttaac aataaagtgt ttattaatag aatcctttgc
     1561 atgatttaca tgtttatgta catgtgttga tttgtatgaa gtaataactt tcttgtagtg
     1621 ggtttgcgta gtaatgaagt ggtggtttac tttgttttgc gtaataaatc tatttacttc
     1681 tgggccttta gcaacaaaat gcttatcaac ttgttgtgaa gctggattgt ttgttgctac
     1741 attcacgcga ttattgattt ctttgtactc tggtacaatg tttcctaact ttgattctgg
     1801 tacggcaaag tttttatcta caattttact agctgccgat gaatctacaa tcttattcaa
     1861 tttgtcttca gaaactacaa agtttttatt gtcagttaaa ttactaacat ttacttttgg
     1921 cgaattctta aattcatcgt aagctggaat aattttatta attcctgatt ctggaactac
     1981 aaattttttg tcaacactct ctgtcactga tgctttagcg tgatttgaat tgataagttg
     2041 tgtagcaaat agcgtgctca tagttaaaac taataatttc tttttcatct gttat
//
LOCUS       DAHHSC010000279         2064 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.283, whole genome shotgun sequence.
ACCESSION   DAHHSC010000279 DAHHSC010000000
VERSION     DAHHSC010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2064)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2064)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2064
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.283"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            <1..888
                     /gene="graS"
                     /locus_tag="NBI57_002557"
                     /gene_synonym="apsS"
     CDS             <1..888
                     /gene="graS"
                     /locus_tag="NBI57_002557"
                     /gene_synonym="apsS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase GraS/ApsS"
                     /protein_id="HCD1823478.1"
                     /translation="SLTMIFLILTYFKEVKLYKHFDKDKEIEEIKHKDLAETPFQRHT
                     VDYLYRQISAHKEKVVEQQLQLNMHEQTITEFVHDIKTPVTAMKLLIDQEKNQERKQA
                     LLYEWSRINSMLDTQLYITRLESQRKDMYFDYVSLKRMVIDEIQLTRHISQVKGIGFD
                     VDFKVDDYVYTDTKWCRMIIRQILSNALKYSENFNIEIGTELNDQHVSLYIKDYGRGI
                     SKKDMPRIFERGFTSTANRNETTSSGMGLYLVNSVKDQLGIHLQVTSTVGKGTTVRLI
                     FPLQNEIVERMSEVTNLSF"
     gene            1032..1793
                     /gene="vraF"
                     /locus_tag="NBI57_002558"
     CDS             1032..1793
                     /gene="vraF"
                     /locus_tag="NBI57_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein VraF"
                     /protein_id="HCD1823479.1"
                     /translation="MAILEVKQLTKIYGTKKMAQEVLRDINMSIEEGEFIAIMGPSGS
                     GKTTLLNVLSSIDYISQGSITLKGKKLEKLSNKELSDIRKHDIGFIFQEYNLLHTLTV
                     KENIMLPLTVQKLDKEHMLNRYEKVAEALNILDISDKYPSELSGGQRQRTSAARAFIT
                     LPSIIFADEPTGALDSKSTQDLLKRLTRMNEAFKSTIIMVTHDPVAASYANRVVMLKD
                     GQIFTELYQGDDDKHTFFKEIIRVQSVLGGVNYDL"
     gene            1783..>2064
                     /locus_tag="NBI57_002559"
     CDS             1783..>2064
                     /locus_tag="NBI57_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCD1823480.1"
                     /translation="MTFNEIIFKNFRQNLSHYAIYLFSLITSVVLYFSFVALKYAHKL
                     NMTESYPIIKEGSQVGSYFLFFIIIAFLLYANVLFIKRRSYELALYQTLG"
BASE COUNT          773 a          274 c          367 g          650 t
ORIGIN      
        1 agtttaacaa tgatttttct tatattgaca tattttaaag aagtaaaatt atataagcat
       61 tttgacaaag ataaagaaat agaagaaatt aaacataaag atttagcgga aacgccattt
      121 caacgtcata cagttgatta tttatatcgt caaatctcag cgcacaaaga aaaggttgtt
      181 gagcaacagt tacaattgaa catgcatgaa caaaccatta cagaatttgt gcacgacata
      241 aaaacacctg tgacagccat gaaattatta attgatcaag aaaaaaatca agaaagaaaa
      301 caggcattac tatatgaatg gtctcgtata aactcgatgc tggatacaca gctgtatatt
      361 actagattag aatctcaacg caaagatatg tattttgatt acgtgtcact taaacgcatg
      421 gtcattgatg aaatacaatt aacaagacat attagtcagg ttaaaggtat tggttttgat
      481 gttgacttta aagtggatga ttatgtttat acagatacaa aatggtgtcg tatgattatt
      541 agacagattt tgtcaaacgc attgaaatat agtgagaatt ttaatattga aattgggaca
      601 gaattaaatg atcaacatgt ttcgttatat attaaagact atggcagagg tattagtaaa
      661 aaagatatgc cgcgaatatt tgaacgagga tttacgtcaa cggctaacag aaatgaaacg
      721 acgtcttcag gtatgggtct atatttagta aatagtgtaa aggatcaatt aggtattcac
      781 ctgcaagtca cgtcgactgt tggtaagggg acaactgtca gattgatttt cccattacaa
      841 aatgaaattg ttgaacgcat gtcggaagtg acaaatttgt cattttaaac atgcgttttg
      901 ttacttagaa ttgatacatc aatgcagctt caacgttata ataagataga tgttagtcat
      961 atgttaaatt gaagatacaa gtgccaaagc ctaaaggaaa tgaagttaag ataaattata
     1021 ggagtgttaa agtggcaatt ttagaagtaa aacaattaac aaaaatatat ggaactaaaa
     1081 aaatggcaca agaagtgttg cgagatatca atatgtctat tgaagaaggc gagtttattg
     1141 ctattatggg tccctctgga tctgggaaaa cgacattatt aaatgtttta agttcaattg
     1201 attatatttc acaaggttct attacattaa aaggaaaaaa attagaaaag ctttcaaaca
     1261 aggaattatc tgatatacgc aagcatgata ttggttttat ttttcaagag tataatttac
     1321 tgcatacatt gactgttaaa gaaaacataa tgttaccact aacggttcag aagttagata
     1381 aagaacatat gttaaatcgt tatgaaaaag tagcagaagc attaaatata ttggatatta
     1441 gtgataaata cccttctgaa ttgtctggtg gacaaagaca acgaacatct gctgcaagag
     1501 cgtttattac attaccttct attatatttg ctgacgaacc aacaggtgca ctggattcta
     1561 aaagtactca agatttatta aaacgattaa caagaatgaa tgaagcattt aagtctacaa
     1621 ttattatggt aacgcatgat cctgttgcag caagttatgc caatcgagta gtgatgctaa
     1681 aagatggtca aattttcact gaattatacc aaggggatga cgataaacat acctttttca
     1741 aagaaataat acgtgtacaa agtgttttag gtggcgttaa ttatgacctt taacgagata
     1801 atatttaaaa atttccgtca aaatttatca cattatgcca tctatctttt ttcattaatt
     1861 acgagtgtag tattgtattt tagctttgta gcattaaaat acgcgcataa actaaacatg
     1921 acagagtcat atccaattat taaggaaggc tcacaagtcg gaagctactt tctatttttc
     1981 atcataattg catttttgtt atatgccaat gtgttattta ttaaacgacg aagttatgag
     2041 cttgcattat atcaaacatt aggt
//
LOCUS       DAHHSC010000280         2030 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.284, whole genome shotgun sequence.
ACCESSION   DAHHSC010000280 DAHHSC010000000
VERSION     DAHHSC010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2030)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2030)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2030
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.284"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            1..702
                     /locus_tag="NBI57_002560"
     CDS             1..702
                     /locus_tag="NBI57_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002464795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HCD1823481.1"
                     /translation="MLSQFLPLLIVKKLPFVQYSGIELTKAVIYIQLVLFLIAATTII
                     LINLKIKNPTKLELEVKEPKKYIIPWALLGFALVMIYQMVVSIVLTQIYGGQQVSPNT
                     EKLIIIARKIPIFIFFVSIIGPLLEEYVFRKVIFGELFNAIKGNRIVAFIIATTVSSL
                     IFALAHNDFKFIPVYFGMGVIFSLAYVWTKRLAVPIIIHMLQNGFVVIFQLLNPEALK
                     KATEQANFIYHIFIP"
     gene            complement(727..1986)
                     /locus_tag="NBI57_002561"
     CDS             complement(727..1986)
                     /locus_tag="NBI57_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1823482.1"
                     /translation="MSYHWFKKMLLSTSILILSSSSLGLATHTVEAKDNLNGEKPTTN
                     LNHNITSPSVNSEMNNNETGTPHESNQTGNEGTGSNSRDANPDSNNVKPDSNNQNPST
                     DSKPDPNNQNPSPNPKPDPDNPKPKPDPKPDPDKPKPNLDPKPDPDNPKPKPDPKPDP
                     DKPKPNPDPKPDPDKPKPNPNPKPDPNKPNPNPSPDPDQPGDSNHSGGSKNGGTWNPN
                     ASDGSNQGQWQPNGNQGNSQNPTGNDFVSQRFLALANGAYKYNPYILNQINKLGKDYG
                     EVTDEDIYNIIRKQNFSGNAYLNGLQQQSNYFRFQYFNPLKSERYYRNLDEQVLALIT
                     GEIGSMPDLKKPEDKPDSKQRSFEPHEKDDFTVVKKQEDNKKSASTAYSKSWLAIVCS
                     MMVVFSIMLFLFVKRNKKKNKNESQRR"
BASE COUNT          559 a          329 c          354 g          788 t
ORIGIN      
        1 atattgtctc aatttttacc gcttctcatt gtaaaaaaat taccatttgt acaatatagt
       61 ggcatagaac tgactaaagc agtcatttac atacaacttg ttctattttt aatcgccgcc
      121 acgacgatta ttttaattaa tttaaaaatt aaaaatccaa caaaattaga attagaagtt
      181 aaagaaccta aaaaatatat cattccatgg gcattgcttg gatttgcatt ggtaatgatt
      241 tatcaaatgg tagtgagcat tgtattaacg caaatttatg gtggacaaca agtaagtcct
      301 aatacagaaa agctaattat tattgctcga aaaataccta tatttatctt ctttgtatct
      361 attatcggtc ctttattaga agaatacgta ttcagaaaag taatctttgg agaattattt
      421 aatgcgatta aaggtaatcg tatcgtagca tttattattg ctacaacagt aagttcatta
      481 atatttgcat tagcacataa tgatttcaaa tttattccag tttattttgg tatgggcgtc
      541 attttttcat tagcatatgt atggacaaaa cggcttgctg ttccaattat tatccatatg
      601 ttacaaaacg gatttgtcgt tatattccaa ttacttaatc ctgaagcttt gaaaaaagcc
      661 acggaacaag cgaattttat atatcacatt tttattccat aatataaaaa acgaatcaca
      721 cacggattat cgtcgctgtg attcgttttt atttttcttt ttatttcgct ttacaaataa
      781 gaatagcatg attgaaaata ccaccatcat agaacataca attgctagcc aacttttact
      841 atatgcagtt gacgcacttt tcttattatc ttcttgtttt ttaactactg taaaatcgtc
      901 tttttcatgc ggttcaaatg agcgttgttt tgaatccggc ttatcttcgg gctttttcaa
      961 atctggcatt gatccaattt caccagtaat taatgcgagt acttgttcat ctaaattacg
     1021 atagtacctt tctgatttca atggattgaa atattggaat ctaaagtaat tcgattgctg
     1081 ttgtaatcca tttaaatatg catttccgct gaaattttgt tttcgaataa tattataaat
     1141 gtcttcatca gtaacttctc cataatcttt gcccaactta ttaatttgat ttaaaatata
     1201 cggattatac ttgtaagccc catttgccaa ggctaaaaat cgttgggata caaaatcatt
     1261 accagtagga ttttgtgagt ttccttgatt cccatttggt tgccattgac cttgattaga
     1321 tccatctgaa gcatttgggt tccatgttcc cccatttttc gagccaccag aatgatttga
     1381 atccccaggt tgatcgggat ctggtgacgg gttaggattt ggcttattag ggtctggttt
     1441 tggattcgga tttggctttg gtttatctgg atctggtttt ggatccggat ttggctttgg
     1501 tttatctggg tctggttttg gatctggttt tggtttcggg ttatctggat ctggttttgg
     1561 atccagattt ggctttggtt tatctgggtc tggttttgga tccggttttg gtttcgggtt
     1621 atctggatct ggttttggat tcggacttgg gttttggtta tttgggtctg gttttgaatc
     1681 tgtacttggg ttttggttgt ttgagtctgg cttcacatta ttcgaatcag gattagcatc
     1741 acgactattc gaacctgttc cttcattacc cgtttgattt gattcgtgag gtgtcccagt
     1801 ctcattatta ttcatttcac tatttactga tggtgaagtt atattatgat tcaaattagt
     1861 agttggtttt tctccattta agttatcctt tgcttcaact gtgtgcgttg caagccctaa
     1921 actactacta cttaaaatta aaatacttgt tgaaagtaac attttcttaa accaatgata
     1981 tgacatttca cgagacacct ccctttttca tgtaagtatt aatctaagta
//
LOCUS       DAHHSC010000281         2025 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.285, whole genome shotgun sequence.
ACCESSION   DAHHSC010000281 DAHHSC010000000
VERSION     DAHHSC010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2025)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2025)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2025
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.285"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            complement(115..759)
                     /locus_tag="NBI57_002562"
     CDS             complement(115..759)
                     /locus_tag="NBI57_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HCD1823483.1"
                     /translation="MSQNTNHSYYHQNQHAQSISKVWLYFMYYWIIFGIGCYLGQFLP
                     LSWRQPLSFGLLIIILATLVFERARRFGLIISHIYAVVIGLLSYATFTTYLQNLGPDI
                     FYKNIALAIFAFIAFGIIGYFFVGDASSIGKYLFVTLITLIIASLIGIFLQNPIFYTI
                     ITVVSLLLFLLYTLYDFNRLKRGDYSPREMGFNLFINLLNIIKDILYLANMFRR"
     gene            1120..1917
                     /locus_tag="NBI57_002563"
     CDS             1120..1917
                     /locus_tag="NBI57_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HCD1823484.1"
                     /translation="MKKLAFAITATSGAAAFLTHHDAQASTQHTVQSGESLWSIAQKY
                     NTSVESIKQNNQLDNNLVFPGQVISVGGSDAQNTSNTSPQAGSASSHTVQAGESLNII
                     ASRYGVSVDQLMAANNLRGYLIMPNQTLQIPNGGSGGTTPTATTGSNGNASSFNHQNL
                     YTAGQCTWYVFDRRAQAGSPISTYWSDAKYWAGNAANDGYQVNNTPSVGSIMQSTPGP
                     YGHVAYVERVNGDGSILISEMNYTYGPYNMNYRTIPASEVSSYAFIH"
BASE COUNT          753 a          340 c          282 g          650 t
ORIGIN      
        1 tcagtaaaat ctctatctaa atttctaatt tatgacaatc gctccaattt aaggttgata
       61 ttcattattg attattccaa aactttatac aaatttcttt attaatttaa ctgtttatct
      121 tctgaacata ttagcaaggt aaagtatatc cttaataata ttcaacaaat taataaatag
      181 attaaatccc atttctcttg gtgaatagtc acctcttttt aaacgattaa aatcatacaa
      241 agtataaagt agaaataaca acagactaac gacggtaata atagtgtaaa aaataggatt
      301 ttgaagaaaa ataccaatta gactcgcaat aattaatgtt attaatgtaa cgaataaata
      361 tttgcctata ctcgatgcat ctccaacgaa gaaataacca ataataccaa atgctataaa
      421 tgcaaaaatt gctaatgcga tatttttata gaaaatatct ggtcctaaat tttgtaaata
      481 cgtggtaaac gttgcgtatg acaataagcc aatcactaca gcgtaaatat gtgaaataat
      541 taaaccgaac cgtctcgctc tttcaaaaac aagtgttgct aaaataataa tcagtaatcc
      601 aaatgacaag ggttgtcgcc aacttaatgg taaaaactga cctagatagc atcctatgcc
      661 aaatataatc caataataca taaaataaag ccacacttta cttattgatt gagcatgctg
      721 gttttgatga taatatgaat gatttgtatt ttgcgacaaa ataattcctc cttctaccgt
      781 cgttttatgc gtcaaatttt acagtaaatt ttgcttcatc aaaagaaata acctttaaca
      841 agtatagtaa ttttacattt tacaatgtta caaaatataa ttttttataa ttagttaaaa
      901 tcactaaaac gcttttatac actatcaaat cagcatttat aaaaatatga accgatatcc
      961 taaaatgtta ataatattac aagataataa caaaccacac aaagctactt atttttgata
     1021 atatggaaat cgtaatataa aacgaaaact taatttacta tataaattgt cttaataatt
     1081 tttaaaagta gtaaaactta attttaagga ggagtccctt tgaaaaaatt agcatttgca
     1141 ataacagcaa catctggtgc agctgcattt ttaacgcatc atgatgcaca agcttctaca
     1201 caacatacag tacaatctgg tgaatcatta tggagtattg ctcaaaaata caacacttca
     1261 gtagagagta ttaaacaaaa taaccaatta gataacaact tggtattccc tggtcaagtt
     1321 atctcagtag gtggaagtga tgcacaaaat acgtcaaaca cttctccaca agctggttca
     1381 gcatcatctc atactgtaca agctggtgaa tcattaaata tcattgctag cagatatggt
     1441 gtttcagttg atcaattaat ggcagccaat aacttacgtg gttatttaat tatgcctaac
     1501 caaacattac aaattcctaa tggtggatca ggtggtacaa caccaacagc tacaacaggt
     1561 agcaatggca atgcatcatc ttttaatcac caaaatttat acactgctgg tcaatgtaca
     1621 tggtacgtat ttgaccgtcg tgctcaagct ggtagtccaa ttagcacata ttggtcagac
     1681 gctaagtatt gggctggtaa cgcagctaat gatggttacc aagtaaacaa cacaccatca
     1741 gttggttcaa ttatgcaaag cacacctggt ccatatggtc atgttgctta tgttgaacgt
     1801 gtcaatggtg atggtagtat cttgatttct gaaatgaatt acacatatgg tccatacaat
     1861 atgaactacc gtacaattcc agcttcagaa gtttctagct atgcattcat ccattaatta
     1921 aataaattgt actgatatat actagcaatt cacatcatgt gagattgcta gttttttatt
     1981 tttgaaaaaa attttcattt tggtacaaaa aattatctca ccctt
//
LOCUS       DAHHSC010000282         2024 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.286, whole genome shotgun sequence.
ACCESSION   DAHHSC010000282 DAHHSC010000000
VERSION     DAHHSC010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2024)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2024)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2024
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.286"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            complement(191..1198)
                     /locus_tag="NBI57_002564"
     CDS             complement(191..1198)
                     /locus_tag="NBI57_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HCD1823485.1"
                     /translation="MSYIIIVTIAVVIFSLIFDFINGFHDTANAVATAVSTRALTPKT
                     AILMAAVMNFIGALTFTGVAGTITKDIVDPFKLENGLVVVLAAILAAIIWNLATWFYG
                     IPSSSSHALIGSIAGAAIASEGSFGVLHYQGFTKIIIVLIVSPIIAFCVGFLMYSIFK
                     VIFKNANLTRANRNFRFFQIFTAALQSFSHGTNDAQKSMGIITLALIVANVQNDGSVE
                     PQLWVKFACATAMGLGTAIGGWKIIKTVGGNIMKIRPANGAAADLSSALTIFVASSLH
                     FPLSTTHVVSSSILGVGASNRAKGVKWSTAQRMIITWVITLPISALLAGLLFYILNLF
                     F"
     gene            complement(1214..1831)
                     /locus_tag="NBI57_002565"
     CDS             complement(1214..1831)
                     /locus_tag="NBI57_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 domain-containing protein"
                     /protein_id="HCD1823486.1"
                     /translation="MFSKKKDKFMVQLEEMVFNLDRAAIEFGKMDFNTHLDLKAYSDN
                     IKTYESHGDELVHQVITDLNQTFITPIEREDILSLCDAIDDVLDAIEETAAMFEMYSI
                     EYTDEYMAEFVDNIQKAVAEMKLAVGLLVDKKLSHMRIHSINIKEFETNCDGILRQSI
                     KHIFNSETDPITLIKIKDIYESMEEIADKCQIVANNFETIIMKNS"
BASE COUNT          686 a          364 c          289 g          685 t
ORIGIN      
        1 cttatatttt aatgacctaa ctgtttatgt tccatggaat aaatttataa cttagccccc
       61 taattaaaat gctttgtctt ggtcattcta cgtaaattct ataaaatatg ttatctactt
      121 acataaaaaa atgaacttca ataccaccat atgtttgtga tactgaagtt cagtttagtt
      181 ttattttcaa ttagaaaaat aagttaagta tatagaatag taaacctgct aacaatgctg
      241 aaataggtaa tgtaatcacc catgtaatga tcattcgttg cgcagtgctc cattttacac
      301 ctttagctcg gttagaagca ccaacaccta agattgatga tgacacaacg tgagttgttg
      361 ataatgggaa atgtagcgat gatgcaacaa aaattgttaa tgcagatgat aaatcggccg
      421 cagcaccatt tgctggacgt attttcataa tattaccacc tacagttttg ataattttcc
      481 agccaccaat tgcagtacca agccccattg ctgtcgcaca ggcaaatttt acccataact
      541 gtggttcaac actgccatca ttctgtacat tagcgacaat caatgccaac gtaataatac
      601 ccattgattt ttgcgcatca ttcgtaccgt gagagaatga ttgtaacgct gccgtgaaaa
      661 tttggaaaaa tctaaagtta cgattcgctc ttgttaaatt tgcattttta aagataactt
      721 taaaaattga atacatcaag aaaccaacac aaaatgcgat tatcggtgaa acgattaata
      781 caataataat ttttgtgaaa ccttggtaat gtaacactcc aaatgagcct tcagatgcga
      841 ttgctgcacc cgcaattgaa cctataagtg catgtgaaga cgaacttgga attccgtaaa
      901 accaagtagc taaattccaa ataatagccg caagtattgc agctaacaca acaactaatc
      961 cattttccaa tttaaatgga tcgacaatgt ctttagtaat ggtgcctgca acgcccgtaa
     1021 atgttaaagc acctataaag ttcatcactg ctgccattaa aattgccgtt ttaggcgtta
     1081 acgctctagt agatacagca gtagctactg cattggctgt atcatggaat ccattgataa
     1141 agtcaaatat cagcgagaaa ataactacag ctatagtgac gatgattata tatgacataa
     1201 atatatactc cccttagcta tttttcataa taatagtttc aaaattattt gctacgattt
     1261 gacatttatc agcaatttct tccatgcttt cataaatatc ttttatttta attaatgtga
     1321 ttggatctgt ttcgctattg aaaatatgtt taattgactg tcttaaaata ccatcacagt
     1381 ttgtttcaaa ttctttaata ttaattgaat gaatacgcat atgtgataat tttttatcga
     1441 ctaataagcc gacagcaagt ttcatttctg caactgcttt ttgaatgtta tcaacaaact
     1501 cagccatata ttcatctgtg tattcgattg aatacatttc aaacatagct gccgtttctt
     1561 caattgcatc taaaacatca tcaattgcat cacataatga taaaatatct tcacgttcaa
     1621 ttggtgtgat aaatgtttga tttaaatcag taattacttg atgtactaat tcgtcaccat
     1681 gtgactcata agttttaatg ttgtctgagt atgcttttaa atctaaatgt gtattgaaat
     1741 ccattttacc gaattcaata gcagcacgat ccagattgaa aaccatctct tctaattgaa
     1801 ccataaactt atcttttttc ttactaaaca tttaaaatcc tccatttaag cgattgtcac
     1861 caatcacatt cagttataat ttgtttcaaa ttaagacaag tgaatttaca aactaatgat
     1921 acaaatttgt tattatcaat cgtcagtata attttagtgt actgatatta atttcaaaaa
     1981 tgcctcacag taaacaattt actgtatttg cccttatatg aaaa
//
LOCUS       DAHHSC010000283         2011 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.287, whole genome shotgun sequence.
ACCESSION   DAHHSC010000283 DAHHSC010000000
VERSION     DAHHSC010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2011)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2011)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2011
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.287"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            120..551
                     /locus_tag="NBI57_002566"
                     /pseudo
     CDS             120..551
                     /locus_tag="NBI57_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001086052.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            588..1376
                     /locus_tag="NBI57_002567"
     CDS             588..1376
                     /locus_tag="NBI57_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1828 domain-containing protein"
                     /protein_id="HCD1823487.1"
                     /translation="MTTFDAKKLKKEYLDWYNQILEFSNLSNNSVKIDTPFKDNSLDN
                     LKIYALYDQSRDMITLTDDGYTIFGLENNGVSINKFKKRKKIFEEHLSAYGIKYNDKT
                     HEIFVQTNFKNFNKSKHNLLQCLIFVNDMYLLSNPKSQNIFTEDVANKLDEHNIYYGR
                     DIPIIGSSGVVHNFDFFISAKKNQKEKFINVFSNPNNSMIIKSKITDAMQAKKIKRHR
                     QNEFIFILNDSKKEINEHNKNLLHENYISTIDYSELDEKIGLLI"
BASE COUNT          819 a          254 c          280 g          658 t
ORIGIN      
        1 tactaaattg atgatataca cttttggttg tgtatgcatc tcacctatct ttttttgctt
       61 ttttcaaata agaaagtatt aaaaattaat gcctatacaa cgttaaagga ggaacagaca
      121 tggctaaact tgatttaaat agtcttgacg atgagcacgt aaaattatta ataaatgaat
      181 taaaatatcc agaaactcat atcgatgtaa atgaataaaa aacaatagtt gctagtcgaa
      241 taaatgaaag gcaagaaata ataagtttta agttaggaat aaagtacttc ttaacaataa
      301 aaagggataa catagaaaaa gataggtttt caatttcaat cattttcaaa gatacctatc
      361 acaccctagt tagaatagat attaacggtg gtactcacga taatccagat ggaacaatcg
      421 ctccgaaaag tcatattcac atatataatg ataagtgtga taaaaaggat aggtttgctt
      481 atgaaattaa cttgaaagat ttccccgaca tctataactt gtataatgtg tatatgtcgt
      541 ttttagagta aaaaaatata aaagaccttg aataaaggag ggataatatg actacgtttg
      601 atgctaaaaa attaaaaaaa gaatatcttg attggtataa tcagatttta gagttttcta
      661 atttatcaaa caattcagta aaaatagata ccccttttaa agataattct ttagataatt
      721 taaaaatata cgctttatac gatcagtcca gagacatgat tacactgact gatgacggct
      781 atactatatt tggtttagaa aataacggtg tttctataaa taaatttaaa aaacgtaaaa
      841 agatttttga agagcacctt tcagcttacg gtattaaata taacgataaa actcacgaaa
      901 tttttgttca aactaacttt aaaaatttta ataaatcgaa acataattta ttacagtgcc
      961 ttatatttgt taatgatatg tacttacttt ctaatcctaa gtcacagaac atatttacag
     1021 aagatgttgc aaacaaattg gatgaacata acatttatta cggaagagat atacctatta
     1081 taggaagcag tggtgttgtt cataatttcg acttttttat tagcgctaag aaaaatcaaa
     1141 aagaaaaatt tatcaatgtt ttttctaacc ctaataattc tatgattatt aagtcgaaaa
     1201 taacggatgc tatgcaagca aaaaaaataa aaagacacag gcaaaatgag tttattttta
     1261 ttttaaatga ctcaaaaaaa gaaataaatg aacataataa aaatcttctt catgaaaact
     1321 atattagtac aatagattat agcgaattag atgaaaagat aggtttattg atttaatatt
     1381 tatagacgtg ataatatcaa tgtttatatt aaatgaaacg aatttgaaaa tttcgaaaca
     1441 gcttaagata gcaaattgaa tagtcttata gataatgcag aatcgtctac acatagttaa
     1501 acaaattatt tgagaataat tattgttaaa ctatgtggat aggcgatttt attttgacaa
     1561 gagtcagaag aacatatatt aaggatttca aattacaaat ggctaaactt tatgaaaatg
     1621 gtatgactag aatcgaaatt atacgcgaat acgatttaac actctcaatc ttctcgaact
     1681 gaataaatac tgggtcattc aatcattaag acaacttaat aagtgatgat atatgttcat
     1741 accattttgc aaatataacc agatactctc aacaaaatgt agagttcaaa attaaaaatg
     1801 tcaacattat agtaaactaa catagtcccc tgttcatatt cttcaatctt taaatcttat
     1861 cgaacaaata aaagcagata attttttaat ttgaacgttt tattttagaa caaaaaagag
     1921 caaggttaac gagtcagaaa atctttttct tgatacgtat atgacacttg ctcttttaag
     1981 aatctgaata tttcacgcta tttccattaa a
//
LOCUS       DAHHSC010000284         2007 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.288, whole genome shotgun sequence.
ACCESSION   DAHHSC010000284 DAHHSC010000000
VERSION     DAHHSC010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2007)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2007)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2007
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.288"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            <1..555
                     /locus_tag="NBI57_002568"
     CDS             <1..555
                     /locus_tag="NBI57_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HCD1823488.1"
                     /translation="IFHLILFIMWGEHQEYWYLYTGIMLIAGISYVFYQRDIESKRLL
                     TSIGVGIITAIILIMLQLLFSLITSNLSYSSLIKELARTGVNWKWQMLVTLLFVIPCH
                     ELYMRTVLQKELTHFSIPKWIAILITAICSSSLFIYLDNWWIVTFIFVAQVILSLSYE
                     YTRRIATTSVAQIVAIILLLIFNA"
     gene            787..932
                     /locus_tag="NBI57_002569"
                     /pseudo
     CDS             787..932
                     /locus_tag="NBI57_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500662.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="putative holin-like toxin"
     gene            1471..1572
                     /locus_tag="NBI57_002570"
     CDS             1471..1572
                     /locus_tag="NBI57_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1823489.1"
                     /translation="MKFVANKLTLTIIRKFCINNEKEIWIKYEEVFL"
     gene            1582..1854
                     /locus_tag="NBI57_002571"
     CDS             1582..1854
                     /locus_tag="NBI57_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001790097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactococcin 972 family bacteriocin"
                     /protein_id="HCD1823490.1"
                     /translation="MFGTILYLTLALGLSTAAYASTEYAEGGTWSHGVGSKYVWSYYY
                     HGHKGHGATAIGKYRSFSGYTRAGVKAKASATKHNCWVNRAYYNIY"
BASE COUNT          697 a          254 c          328 g          728 t
ORIGIN      
        1 atatttcatc tcatattatt tattatgtgg ggcgaacacc aagaatactg gtatttatat
       61 acaggtataa tgctaattgc tggtatcagt tatgtatttt atcaaagaga tattgaatct
      121 aagcggttgc ttacatcaat tggtgttggt attattacgg caattatttt aattatgctt
      181 caacttttat tctcacttat aacttctaat ttaagttata gttcattaat taaagaatta
      241 gcaagaacag gtgtcaattg gaagtggcaa atgttagtaa ctttactttt tgtcattcca
      301 tgtcacgagt tatatatgag aactgtttta caaaaagaat taacacattt ttcaataccg
      361 aaatggatag ctatattaat aactgcaatt tgttctagtt cattatttat ttatttagat
      421 aattggtgga tagtaacatt tatatttgtt gcacaagtga tattatcatt aagttatgag
      481 tatactcgac gcatagcaac aacatccgtt gcgcaaattg ttgctattat attgttatta
      541 atttttaatg cttaaataag cttttcttag acgtctgtgt tctaagaaaa gctttttgct
      601 ttagaaaagc aatacgaaga ttcaaggcat gctttattat agtgtataca tttttaacgg
      661 caagcagtta ttattgaggt tatattttag tcagttttta ttactaaata ttttataaaa
      721 atagtataat attttgtggg caagttgaat tgatggtggc tatctgagta aaggggggtg
      781 gtacctatgg cattacttaa cacttttgaa aggaaaagcc tattgtgata tctattgcaa
      841 acgcattaca tttaatgtta agtttcggta tgtttatcgt cactttcatt ggtatagtag
      901 tagcaataat aaatttaagc aataaaaaat aaccatcatt tcaaaacttt gacatagaat
      961 gatggttaaa gaaaatctca tttagtcacc gtctttttaa cgggctcatt aggcgacatg
     1021 ttcctgcatg tcgtcttttt acatttttat agtaacatat cttttattta attgtctatt
     1081 tttattagat gtgtaacaaa attttgaagt attttccgta tattgcctgc taaacatgtc
     1141 agatttgaaa ggaaaatcat actgtggtag aaatcaatta tgattatatg gtagtactta
     1201 taaataagtg atttctattg aatatgtaat gaagttcata tattaatata actttcattg
     1261 atatagaagt cgcaatgatt aatttaaaca ataaaaaaca accatcattt caaaactttg
     1321 acaacgaatg atggttaaag aaaatctcat ttagtcaccg tctttttaac gggttcaata
     1381 ggggacatgt tcctgcatgt cgtctattta tgcactgcgc aatttaacaa taatacaatt
     1441 atactttgat gtccatttaa cataaagtgg ttgaaatttg tagcaaataa attaaccttg
     1501 actattatca gaaaattctg tataaataac gaaaaagaga tatggattaa atatgaagag
     1561 gtgtttttat agaagaataa aatttttggc actatattat atttaacttt agcacttgga
     1621 ttatcaacag cagcttatgc atctacagaa tacgcagaag gaggcacttg gagtcacggt
     1681 gtcggcagta agtatgtttg gtcttattat tatcatggtc ataaaggaca tggtgcaaca
     1741 gctattggaa aatatagatc atttagtggt tatacaagag ctggtgtaaa agcaaaagca
     1801 tcagctacta aacataattg ctgggtcaat agagcgtatt ataacattta ttaaatacta
     1861 aaaaatcata gaaaataaac aacgcaagag gtacaacatg aaatggttca aattaatact
     1921 tgatgttaca accttcattc tgattgctat attgttattt gtttatacat ataaagaaaa
     1981 cgaagaaata ttgcctgata ctaaata
//
LOCUS       DAHHSC010000285         1988 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.289, whole genome shotgun sequence.
ACCESSION   DAHHSC010000285 DAHHSC010000000
VERSION     DAHHSC010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1988)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1988)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1988
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.289"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            <1..1943
                     /gene="sfaD"
                     /locus_tag="NBI57_002572"
                     /gene_synonym="sfnaD"
     CDS             <1..1943
                     /gene="sfaD"
                     /locus_tag="NBI57_002572"
                     /gene_synonym="sfnaD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="D-ornithine--citrate ligase SfaD"
                     /protein_id="HCD1823491.1"
                     /translation="KFNKEEQETYLFLQQHNSDWANIFKEMILQGRDKVTQRLVTSMH
                     RENLVKARTQSKKILSRDLIMLDISTTHILEIQFPQAKQTLYAPITGEHAFDRIDVEG
                     PFYIKDDITNTITRVHHPNEILECILIEAPDLKNAASDQFQQDLINSATNMTFAISYQ
                     ALSMQHDSAPLFNIIENSEDSYLRSEQAVIEGHPLHPGAKLRKGLNALQTFLYSSEFN
                     QPIKLKIVLIHSKLSRTMSLSKDYDTTVHQLFPDLIKQLENEFTPNFNFNDYHIMIVH
                     PWQLDDVLHSDYQAEVDKELIIEAKHTLDYYAGLSFRTLVPKYPAMSPHIKLSTNVHI
                     TGEIRTLSEQTTHNGPLMTRILNDILEKDVIFKSYASTIIDEVAGIHFYNEQDEVDYQ
                     TERSEQLGTLFRKNIYQMIPQEVTPMIPSSLVATYPFNNESPIVTLIKRYQSAASLSD
                     FESSAKSWIETYSKALLGLVIPLVTKYGIALEAHLQNAIATFRKDGLLDTMYIRDFEG
                     LRIDKAQLNEMGYSTSHFHEKSRILTDSKTSVFNKAFYSTVQNHLGELILTISKASND
                     SNLERHMWYIVRDVLDNIFDQLVLSTHKSNQVNENRINEIKDTMFAPFIDYKCVTTMR
                     LEDEAHHYTYIKVNNPLYRENN"
BASE COUNT          729 a          337 c          308 g          614 t
ORIGIN      
        1 taaaatttaa caaagaagaa caagaaacat atttattttt acaacaacat aatagcgatt
       61 gggctaatat ttttaaagag atgattttac aaggaagaga taaagtcact cagagattag
      121 tcacatcaat gcatcgcgaa aacttagtta aagcgcgcac tcaaagtaag aaaatattga
      181 gtcgtgattt gattatgtta gatatctcaa cgactcatat actagaaatc cagttcccac
      241 aagctaagca aacattatat gctcctatca ctggagaaca cgcctttgat cgaattgatg
      301 ttgaaggccc tttctacatt aaagatgaca ttacaaatac gattacacga gtacaccatc
      361 caaatgaaat tcttgagtgt attttaattg aagcaccaga tttaaaaaat gctgctagtg
      421 atcaatttca gcaagattta ataaacagtg caactaatat gacatttgca attagctatc
      481 aagcactgtc aatgcaacat gattctgcac ctttatttaa tattatcgaa aatagcgaag
      541 atagttactt gcgttcagag caagccgtta ttgaaggtca tccgctacat cctggggcta
      601 aattacgtaa aggtttaaat gcattacaaa cattcctata ttcttcagaa tttaatcaac
      661 ctattaagtt gaaaatagta ctgattcaca gtaaattaag tcgtactatg tcactgtcta
      721 aagattatga tacaactgtt catcagctat ttccggactt aattaaacaa ttagaaaatg
      781 aatttacgcc aaactttaac tttaatgatt atcacattat gattgtacat ccatggcaat
      841 tagatgacgt attacattct gattatcaag cagaagtaga taaagaatta atcattgaag
      901 caaaacatac gttggattat tatgccggtt tatctttcag aacattagtt cctaaatacc
      961 cagctatgtc gccacacatc aaattgtcga caaacgttca tattacaggt gaaattcgta
     1021 cattatcaga acaaacgacg cacaatggtc cactaatgac acgtatttta aatgatattt
     1081 tggagaaaga tgtcatattt aagtcctatg catcaacaat tattgatgaa gttgctggta
     1141 ttcattttta taatgagcaa gacgaagtag actatcaaac tgaacgaagt gaacaattag
     1201 gtacattatt tagaaaaaac atataccaaa tgattccaca agaagtcaca ccaatgattc
     1261 cttcaagctt agttgcaacc tacccattta acaatgaatc acctatcgtg acattgatta
     1321 aacgttatca atcagctgca tcattgtctg attttgaatc atcggcgaaa tcgtggattg
     1381 aaacttatag taaagcattg cttggtctcg taatcccact agttactaaa tatggcattg
     1441 cattagaggc tcacttgcaa aatgctattg caacatttcg gaaagatggt ctattggaca
     1501 cgatgtatat tagagacttt gaaggtttaa gaattgataa agcacaacta aatgaaatgg
     1561 gatacagtac atctcatttc catgaaaaat caagaattct tactgattca aaaacgtctg
     1621 tgtttaacaa agccttttat tctaccgttc aaaaccattt aggcgaatta attcttacaa
     1681 tctctaaagc atcaaatgat tcaaacttag aaagacatat gtggtatatc gttcgtgatg
     1741 tactagataa catttttgat caacttgtac tttcgacaca caaatctaat caagtaaatg
     1801 agaatcgcat aaatgaaatt aaagatacca tgttcgcgcc atttatagat tataagtgtg
     1861 ttacaacaat gcgtcttgaa gatgaggcac atcattatac ttatattaaa gttaataatc
     1921 ctttgtatcg agaaaataat taaaattata gaattttatt aatcgtgagc tcaaggatta
     1981 cattaact
//
LOCUS       DAHHSC010000286         1974 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.290, whole genome shotgun sequence.
ACCESSION   DAHHSC010000286 DAHHSC010000000
VERSION     DAHHSC010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1974)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1974)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1974
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.290"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            95..337
                     /locus_tag="NBI57_002573"
     CDS             95..337
                     /locus_tag="NBI57_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011275855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HCD1823492.1"
                     /translation="MATWLAIIFIVAALILGLIGGFLLARKYMMDYLKKNPPINEEML
                     RMMMMQMGQKPSQKKINQMMTMMNKNMDQNMKSAKK"
     gene            324..422
                     /locus_tag="NBI57_002574"
     CDS             324..422
                     /locus_tag="NBI57_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1823493.1"
                     /translation="MRKSKFAIDRGYFPDMEMASFYNQINKNKYVY"
     gene            516..983
                     /locus_tag="NBI57_002575"
     CDS             516..983
                     /locus_tag="NBI57_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001566616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CcdC family protein"
                     /protein_id="HCD1823494.1"
                     /translation="MLYLIFSIIVALFMGTIVIVIRMKAQNYPVNEKKIVLPPFFMAT
                     GALMYVVPYFRLTGSEMLEAFIIGLLFSTVLIWTSRFEVKGTEIYMKRSKAFPVILIS
                     LLIIRTVMKIFISNEIDPGELGGMFFLLAFCMIVPWRAAMLYKYKKLKKTLIN"
     gene            1107..>1974
                     /locus_tag="NBI57_002576"
     CDS             1107..>1974
                     /locus_tag="NBI57_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HCD1823495.1"
                     /translation="MKIIHTADWHLGKILNGKQLLEDQAYILDMFVEKMKEEEPDIIV
                     IAGDLYDTTYPSKDAIMLLEQAIGKLNLELRIPIIMISGNHDGKERLNYGASWFEHNQ
                     LFIRTDFTSINSPIEINGVNFYTLPYATVSEMKHYFEDDTIETHQQGITRCIETIAPE
                     IDEDAVNILISHLTVQGGKTSDSERPLTIGTVESVQKGVFDIFDYVMLGHLHHPFSIE
                     DDKIKYSGSLLQYSFSEAGQAKGYRRLTINDGIINDVFIPLKPLRQLEIISGEYNDVI
                     NEKVHVKNKDNYL"
BASE COUNT          724 a          251 c          363 g          636 t
ORIGIN      
        1 tagtatcgat attaaaatac ttgaataaac tagttcttga aataatgtga tgaatttagt
       61 aaaattcagt aggataaaga aagcgggtga aacaatggca acttggttag caattatttt
      121 tatagtagct gcattaattt taggtttaat tggaggtttc cttttagcta gaaaatatat
      181 gatggactac ttgaagaaaa acccaccaat caacgaagaa atgcttcgta tgatgatgat
      241 gcaaatgggt caaaaacctt ctcagaagaa aattaatcaa atgatgacga tgatgaataa
      301 aaatatggat caaaatatga agagtgcgaa aaagtaaatt cgcaattgat agaggctatt
      361 ttccagatat ggaaatggcc tctttttata atcaaattaa taaaaacaaa tatgtttatt
      421 aaaattaaag ttaacaaaat gacgaataga ctgagaaatg ctataattca ttttgtatga
      481 tttacagaga gtttatttaa cgagaaggtg tctgcgtgct ctatttaata ttttcaatca
      541 ttgtagcttt atttatggga actatagtta tagttattcg tatgaaagct caaaattatc
      601 cggtaaatga gaaaaaaata gttttgccac cgttttttat ggcgaccggt gcattgatgt
      661 acgtcgttcc atattttagg ctaacaggat cggaaatgct agaagccttt ataattggtt
      721 tgcttttttc tacagttcta atttggactt ctcgatttga agtcaaaggt acagaaattt
      781 atatgaaacg atctaaagca tttccagtta ttttgatttc attacttatc attcgtactg
      841 tgatgaaaat attcattagt aatgaaatag atcctggaga attaggcggc atgttctttt
      901 tattagcatt ctgtatgatt gttccttgga gagcagcaat gctatataaa tacaaaaaac
      961 taaagaaaac attaatcaat taattacttt taaaaccact tgtgatcgac ttctaaatca
     1021 gtcaatgagt ggttttaatt ttacttgaaa aggtgaaatg attatattaa aataaaagcg
     1081 aacaaatgtt ctatatggag gcagtaatga aaattataca tacagcagac tggcacttag
     1141 ggaaaatatt aaatggcaaa cagcttttag aagatcaagc gtatatttta gatatgttcg
     1201 tagaaaaaat gaaagaagaa gaacctgata tcattgtgat agctggagat ttatatgaca
     1261 caacatatcc aagtaaagat gcaatcatgt tattagaaca agcgattgga aagttaaatt
     1321 tagaactgcg tataccaata attatgatta gtggaaatca cgatggtaaa gagaggttaa
     1381 actatggggc gagttggttt gaacataatc agttatttat aagaacagat tttacatcga
     1441 ttaattcacc aatagagata aatggggtta atttttatac actcccttat gctactgtga
     1501 gcgaaatgaa acactacttt gaagatgaca ccattgaaac acatcaacag ggaattacgc
     1561 gctgtattga aacaatagca ccggaaattg atgaagatgc cgtcaatatt ttaattagtc
     1621 atctgactgt tcaaggtgga aagacatctg attctgaaag accattaact attggaacgg
     1681 ttgaatcagt tcagaaaggt gtttttgata tatttgatta tgtcatgcta ggtcacttgc
     1741 atcatccatt tagtatagaa gacgacaaaa ttaaatatag tggctcctta ttgcagtatt
     1801 cattttcgga agcgggtcaa gctaaagggt atagacgttt aacaattaat gatggcatta
     1861 ttaacgatgt atttattcct cttaagccac ttagacaatt ggaaattatc tcaggcgaat
     1921 ataatgatgt tattaatgaa aaagttcatg tgaaaaataa agataattat ttac
//
LOCUS       DAHHSC010000287         1969 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.291, whole genome shotgun sequence.
ACCESSION   DAHHSC010000287 DAHHSC010000000
VERSION     DAHHSC010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1969)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1969)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1969
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.291"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            complement(51..734)
                     /locus_tag="NBI57_002577"
     CDS             complement(51..734)
                     /locus_tag="NBI57_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HCD1823496.1"
                     /translation="MSKSTKKSNDTIVETFKDIIPLSFGEEIGNAASHGVAALLTLLV
                     LPYAAVHSYIAHGTLASVSMSIYVISIFMMFISSTIYHSMQNETPHKYILRIIDHSMI
                     YVAISGTYTPILLTVVGGWIGWTVFILLWGTTLWGILYKSIAVKVNQKLSLIVYLVMG
                     WVGIILLPIIFIRTSWIFILFIFLGGVSYTIGAWFYAQKNRPYFHMIWHIFIVIASLL
                     HLIAILYFM"
     gene            complement(753..1940)
                     /locus_tag="NBI57_002578"
     CDS             complement(753..1940)
                     /locus_tag="NBI57_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDPGP type 1 family protein"
                     /protein_id="HCD1823497.1"
                     /translation="MLDKNQLAKYKQDHLCEYEKIMSNNEKEALEEKVASLDLDFIAK
                     LYNDLYINKKTIDDVSAVSEVKYDIKSQMSDDEIKRLEEQGLQAIKEGQFAVLLMAGG
                     QGTRLGYKGPKGSFEIEGVSLFELQANQLKTLNHQSGHTIQWYIMTSDINHEETLAYF
                     EAHSYFGYDQEAIHFFKQDNIVALSEEGKLILNQQGRIMETPNGNGGVFKSLDKAGYL
                     EEMSNNGVKYIFLNNIDNVLVKVLDPLFAGFTVEHDYDITSKTIQPKPGESVGRLVNV
                     DCKDTVLEYSELDPEVANQFNNANIGIHAFKLGFILNAVNRELPYHLAIKNLKQLDEN
                     FGVIEQPTLKFELFYFDIFTYGTSFVTLQVPREEEFSPLKNKEGKDSVATATEDLRRM
                     GLI"
BASE COUNT          669 a          374 c          254 g          672 t
ORIGIN      
        1 atgatttatg taataaaaaa agcggagttt cccccagctt ttctaaacga ctacataaaa
       61 tataagatcg caattaaatg caatagtgat gctattacaa taaaaatatg ccaaatcata
      121 tgaaaatatg gtctattctt ttgtgcataa aaccatgcac caattgtata agacacacca
      181 cctaagaaaa tgaataatat gaatatccat gatgtgcgaa taaaaataat tggtaacaag
      241 ataataccta cccagcccat cactaaatag acaattaagc ttaacttttg gttaactttg
      301 acagctatgg atttatataa aatcccccaa agtgtcgttc cccataataa aataaatact
      361 gtccaaccta tccaaccacc tacaactgtt aacaaaatag gcgtatatgt accagaaatt
      421 gctacgtaaa tcatactgtg atcgataatc cttaaaatat atttatgagg tgtttcattt
      481 tgcatagaat gataaatagt agatgaaata aacatcataa agatagaaat aacataaata
      541 gacatactca cagacgcaag cgtaccatgt gcaatataac tgtgcacagc cgcatagggt
      601 aacactaata atgttaacaa agctgcaaca ccatgagatg ccgcattacc aatttcttct
      661 ccaaatgata aagggatgat atccttaaat gtttctacga ttgtgtcatt acttttttta
      721 gttgatttgg acatcatagt caccgcctat cattaaatta aacccattct acgtaaatct
      781 tctgttgcag ttgcaacact atctttacct tctttatttt taagaggtga aaattcttct
      841 tctctaggaa cttgtaacgt gacaaaacta gttccataag taaatatgtc aaaatagaac
      901 aattcgaatt ttaaagttgg ttgttcaata accccaaagt tctcatctaa ttgtttcaaa
      961 ttcttaatag ctaaatgata aggtaactca cgatttacag cgtttaaaat aaaaccaagt
     1021 ttaaatgcat gaataccgat attagcattg ttaaattgat ttgcaacttc tggatctaat
     1081 tctgaatact ccagtactgt atctttacaa tcgacattaa ctaaacgtcc aacactttct
     1141 cctggttttg gttgaatcgt tttagaagta atatcgtaat catgttctac cgtaaatcct
     1201 gcaaatagtg gatccaacac ttttactaaa acattgtcga tattattcaa gaaaatatac
     1261 ttaacgccat tattagacat ttcttctaag tatcctgcct tgtctaaaga tttaaacacg
     1321 ccaccattac cattcggcgt ttccataata cgaccctgtt gatttaaaat taacttccct
     1381 tcttcactaa gcgccacaat attatcttgt ttaaagaaat ggattgcttc ttgatcatat
     1441 ccaaaataac tatgtgcttc gaaataagct aaagtttctt catgattgat atcacttgtc
     1501 ataatatacc attgaatcgt atgtccagat tgatgattca atgtttttaa ttggttagct
     1561 tgaagttcaa ataaacttac accttcaatt tcaaacgaac ctttagggcc cttgtaacca
     1621 agtcttgttc cttgaccacc tgccatcaaa agtactgcaa attgtccttc tttaatagct
     1681 tgaagtcctt gttcctctaa acgcttaatc tcatcatcac tcatttgtga tttaatatca
     1741 tatttcactt cagaaactgc agatacatca tctattgttt tcttattgat gtataaatcg
     1801 ttatataatt tggcaataaa atctaaatct aatgacgcta ccttctcttc aagtgcttct
     1861 ttttcattgt tactcattat tttttcatat tcacacaaat gatcttgttt atatttagct
     1921 aattgatttt tatctagcat tttatgcctc ctgctgagtt tgtaacctt
//
LOCUS       DAHHSC010000288         1953 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.292, whole genome shotgun sequence.
ACCESSION   DAHHSC010000288 DAHHSC010000000
VERSION     DAHHSC010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1953)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1953)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1953
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.292"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            <1..178
                     /locus_tag="NBI57_002579"
     CDS             <1..178
                     /locus_tag="NBI57_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HCD1823498.1"
                     /translation="EIREEYIAASNIWNRTLLFTENDKQFKGQAIDLDYDGYLIVRDE
                     AGESHRLISADIDF"
     gene            202..>1953
                     /locus_tag="NBI57_002580"
     CDS             202..>1953
                     /locus_tag="NBI57_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HCD1823499.1"
                     /translation="MGMATYAVVDLETTGNQLDFDDIIQIGITFVRNNQIIDTYHSMI
                     RTNLEIPPFIQALTSIEENMLQQAPYFNQVAQEIYDKIKDCIFVAHNVDFDLNFIKKA
                     FKDCNIQYRPKKVIDTLEIFKIAFPTDKSYQLSELAEAHGITLANAHRADEDAATTAK
                     LMILAFEKFEKLPLDTLKQLYYLSKQLKYDLYDIFFEMVRQYDAKPLDKSYEKFEQII
                     YRKQVDFKKPTTNYNGSLKSLYSKAVDQLGLTYRPQQLYLAETILDQLMHSEKAMIEA
                     SLGSGKSLAYLLAALMYNIETGKHVMISTNTKLLQSQLLEKDIPAMNEALNFKINALL
                     IKSKSDYISLGLISQILKDDTSNYEVNILKMQLLIWITETPSGDIQELNLKGGQKMYF
                     DQKIETYVPARHDVHYYNFIKRNAQNIQIGITNHAHLIHSDVENSIYQLFDDCIVDEA
                     HRLPDYALNQVTNELSYADIKYQLGLIGKNENEKLLKAIDQLEKQRILEKLDIAPIDI
                     FGLKASMNEIHELNEQLFSTIFTIINDSDVYDDDIHRFHNVFTFETKDILKDLHAIID
                     KLNKTLEIFNGISHKTVK"
BASE COUNT          725 a          264 c          347 g          617 t
ORIGIN      
        1 tgaaattcgt gaagaatata ttgcagcttc taatatttgg aatagaacgt tgctatttac
       61 agaaaatgat aaacagttta aaggacaagc aattgattta gattacgatg gctatctaat
      121 tgttagagat gaagcgggtg aatcacaccg tttaattagt gcagatatag atttttaaca
      181 ctaaagcaag gagagatagc tatgggtatg gcaacctatg ccgttgtgga tttggaaaca
      241 acaggcaacc aattagattt tgacgatatc attcaaattg gtataacgtt tgtgcgcaat
      301 aaccaaatta ttgatacata tcattctatg attagaacaa atttagagat tccgccgttc
      361 attcaagcgt taacatccat tgaagaaaat atgctacaac aagcgccata ttttaatcaa
      421 gttgcacaag aaatctacga taagataaaa gattgcattt ttgtcgctca taatgtagat
      481 tttgatttga attttattaa aaaggcattt aaagattgta atattcaata tcgtcccaaa
      541 aaagttatag atacactaga aatctttaaa atagcttttc ctacagataa aagctatcaa
      601 ttaagtgaat tagcagaggc acatggtatt accttagcaa atgcccatcg agctgacgaa
      661 gatgccgcta ctactgctaa attgatgata ttagcttttg aaaaatttga aaagttaccg
      721 ttagatactt tgaagcaatt atattattta agtaaacagt tgaaatacga tttgtatgat
      781 attttctttg agatggttag gcaatatgat gcaaagccat tggataaatc gtacgaaaag
      841 ttcgaacaga ttatctatcg taagcaagtt gattttaaaa agccgacaac aaattataat
      901 ggcagcttga agtctttata tagcaaagct gtagatcaac ttggcttaac atatcgaccg
      961 caacaattat atttagcgga aactatatta gatcagctca tgcatagtga aaaagcaatg
     1021 attgaagcat cactaggcag tggtaaatca ttagcatatt tacttgcggc cttgatgtat
     1081 aatattgaaa ctgggaagca tgtcatgatt tctaccaata ctaaattact gcaaagtcaa
     1141 ttgttagaaa aagatattcc agcaatgaat gaagcgttaa attttaaaat taatgcacta
     1201 ttgattaaaa gtaaaagtga ttacatttca ttgggactta ttagtcaaat tttaaaagat
     1261 gacacaagta attacgaagt gaatatcttg aaaatgcaat tgttaatttg gattacagag
     1321 acgccgtccg gagatataca ggaattaaat ttaaaaggtg gtcaaaagat gtattttgac
     1381 cagaaaattg aaacatatgt accggcacgt cacgatgttc attattataa ttttattaag
     1441 cgaaatgcac agaacattca aattggtatt acaaatcatg cacatttaat tcattctgat
     1501 gttgaaaatt caatatatca attgtttgat gattgtatag tggatgaagc acatcgctta
     1561 ccagactatg ccttaaatca agtgacaaat gagttgagtt atgctgatat taaatatcag
     1621 cttggtttga ttggtaaaaa cgaaaatgaa aaattgttaa aggcaatcga tcaactggaa
     1681 aagcagcgaa ttttggaaaa attagatatt gcaccaattg atatttttgg cttgaaggct
     1741 agtatgaatg agattcatga gttaaatgag caattgttct caacgatatt tacgattatc
     1801 aatgattctg atgtttatga tgatgatatt catcgcttcc ataatgtatt tacttttgaa
     1861 acaaaagaca ttttgaagga tttacatgcg attatcgata agttaaataa aacgctagaa
     1921 atattcaatg gcattagtca taaaacagtt aaa
//
LOCUS       DAHHSC010000289         1944 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.293, whole genome shotgun sequence.
ACCESSION   DAHHSC010000289 DAHHSC010000000
VERSION     DAHHSC010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1944)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1944)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1944
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.293"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            complement(<1..333)
                     /locus_tag="NBI57_002581"
     CDS             complement(<1..333)
                     /locus_tag="NBI57_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactam sensor/signal transducer BlaR1"
                     /protein_id="HCD1823500.1"
                     /translation="MAKLLITSVVSFCFIFLLLVFFKYILKRYFNYSLNYKVWYLTVL
                     AGLIPFIPIKFSFIKLNNVNNQAPTVESKSHDLNHNINTTKPVQEFTTDIHKFNWDSI
                     DNICTVVWI"
     gene            440..1285
                     /gene="blaZ"
                     /locus_tag="NBI57_002582"
     CDS             440..1285
                     /gene="blaZ"
                     /locus_tag="NBI57_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000733274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-hydrolyzing class A beta-lactamase
                     BlaZ"
                     /protein_id="HCD1823501.1"
                     /translation="MKKLIFLIAIALVLSACNSNSPHAKKLNDLEKKYNAHIGVYALD
                     TKSGKEVKFNSDKRFAYASTSKAINSAILLEQIPYNKLNKKIHINKDDIVAYSPILEK
                     YVGKDITLKELIEASMTYSDNTANNKIIKEIGGIKKVKQRLKELGDKVTNPVRYEIEL
                     NYYSPKSKKDTSTPAAFGKTLNKLIANGKLSKENKKFLLDLMLNNKSGDTLIKDGVSK
                     DCKVADKSGQAITYASRNDVAFVYPKGQSEPIVLVIFTNKDNKSDKPNDKLISETAKS
                     VMKEF"
     gene            complement(1639..>1944)
                     /locus_tag="NBI57_002583"
     CDS             complement(1639..>1944)
                     /locus_tag="NBI57_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002455976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1823502.1"
                     /translation="IKKIDNLFKNSKDDYPATVGVMSQNLWYFRYFIYYLIKENVISK
                     KEINSYCMSQKYGSNQKGYKSDLNWNYINSKDNVDEFFSELLEEKVPLIDLNYVNLI"
BASE COUNT          757 a          240 c          258 g          689 t
ORIGIN      
        1 tatccaaaca actgtgcaga tattatcaat tgaatcccaa ttaaacttat ggatatctgt
       61 tgtgaactct tgaacaggtt tggtggtatt tatgttatgg ttcaagtcgt gtgacttact
      121 ttcaactgtg ggcgcttgat tattcacatt attcaatttt ataaaagaga atttaattgg
      181 gataaaagga attaatccag caagcacagt tagataccaa actttataat ttaaactata
      241 attaaaatag cgttttaata tgtacttaaa aaatactaac aatagaaata taaaacaaaa
      301 gcttactacg ctcgttatta gtaatttagc cattttgaca ccttctttca aatatttata
      361 ataaacaatt gacaccgata ttacaattgt aatattattt atttataaaa attacaattg
      421 taatattgga gggtttattt tgaaaaagtt aatattttta attgcaattg ctttagtttt
      481 aagtgcatgt aattcaaaca gtccacatgc caaaaagtta aatgatttag aaaaaaaata
      541 taatgctcat attggtgttt atgctttaga tactaaaagt ggtaaggaag taaaatttaa
      601 ttcagataag agatttgcct atgcttcgac ttcaaaagcg ataaatagtg ctattttgtt
      661 agaacaaata ccttataata agttaaataa aaaaatacat attaacaaag atgatatagt
      721 tgcttattct cctattttag aaaaatatgt aggaaaagat atcactttaa aagaacttat
      781 tgaggcttca atgacatata gtgataatac agcaaacaat aaaattataa aagaaatcgg
      841 tgggatcaaa aaagttaaac aacgtctaaa agaactagga gataaagtaa caaatccagt
      901 tagatatgag atagaattaa attactattc accaaagagc aaaaaagata cttcaacacc
      961 tgctgctttc ggtaagactt taaataaact tatcgcaaat ggaaaattaa gcaaagaaaa
     1021 caaaaaattc ttacttgatt taatgttaaa taataaaagc ggagatactt taattaaaga
     1081 tggtgtttca aaagactgta aggtggctga taaaagtggt caagcaataa catatgcttc
     1141 tagaaatgat gttgcttttg tttatcctaa gggccaatct gaacctattg ttttagtcat
     1201 ttttacgaat aaagacaata aaagtgataa gccaaatgat aagttgataa gtgaaaccgc
     1261 caagagtgta atgaaggaat tttaatattc taaatgcata ataaatactg atatcatctt
     1321 atagtttgta ttatcgttga catctataat tttgatatca aaaactgatt ttccctctat
     1381 tattttcgag atttattttc ttaattctct ttaacaaact agaaatattg tatatacaaa
     1441 aaatcataaa taatagatga atagtttaat tctaggtatt catcaatcga aaaagtaacg
     1501 tatcttattt aaagtgcgtt gcttttttct catttataag gttaaataat tctcatatat
     1561 caagcaaagt gacaaacaac ttattttgtg tgattagggt gtacttaaat aacacaaaag
     1621 taggtttaat ataattaatt atattaaatt tacataattt aaatctatga gtggtacttt
     1681 ttcttctaat aattcactaa aaaattcatc cacattatct tttgaattaa tataattcca
     1741 gtttaaatcg ctcttatatc ctttctgatt tgaaccatat ttttgactca tgcaataact
     1801 attaatttct ttttttgaaa tcacattttc ttttattaaa tagtaaataa agtatctgaa
     1861 ataccataaa ttttgggaca ttacacctac tgttgcagga taatcatcct tagaattttt
     1921 aaatagatta tcaatttttt taat
//
LOCUS       DAHHSC010000290         1930 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.294, whole genome shotgun sequence.
ACCESSION   DAHHSC010000290 DAHHSC010000000
VERSION     DAHHSC010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1930)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1930)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1930
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.294"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            203..1762
                     /gene="rny"
                     /locus_tag="NBI57_002584"
     CDS             203..1762
                     /gene="rny"
                     /locus_tag="NBI57_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002459258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HCD1823503.1"
                     /translation="MNLLSLLLILLGIILGVVGGYVVARNLLLQKQSQARQTAEDIVN
                     QAHKEADNIKKEKLLEAKEENQILREQTEAELRERRSELQRQETRLLQKEENLERKSD
                     LLDKKDEILEQKESKIEEKQQQVDAKESSVQTLIMKHEQELERISGLTQEEAINEQLQ
                     RVEEELSQDIAVLVKEKEKEAKEKVDKTAKELLATAVQRLAADHTSESTVSVVNLPND
                     EMKGRIIGREGRNIRTLETLTGIDLIIDDTPEAVILSGFDPIRREIARTALVNLVSDG
                     RIHPGRIEDMVEKARKEVDDIIREAGEQATFEVNAHNMHPDLVKIVGRLNYRTSYGQN
                     VLKHSIEVAHLASMLAAELGEDETLAKRAGLLHDVGKAIDHEVEGSHVEIGVELAKKY
                     GENETVINAIHSHHGDVEPTSIISILVAAADALSAARPGARKETLENYIRRLERLETL
                     SESYDGVEKAFAIQAGREIRVIVSPEEIDDLKSYRLARDIKNQIEDELQYPGHIKVTV
                     VRETRAVEYAK"
BASE COUNT          742 a          276 c          387 g          525 t
ORIGIN      
        1 cattatcaat agttttataa tcgagcttca aaactttaga aaatagtaga aatagcattc
       61 aatatagtgc aaaagtgcaa attgataact tgacacttat ctcctataaa ccgtacaatt
      121 aatttgtatg atttatatat aatttcataa agtcatattg aatttcatat aaagagcaaa
      181 ccctagaaaa ggaggtgttt gtgtgaattt attaagcctc ctactcattt tgctggggat
      241 cattctagga gttgttggag ggtatgttgt tgcccgaaat ttgttgcttc aaaagcaatc
      301 acaagctaga caaactgccg aagatattgt aaatcaagca cataaagaag ctgacaatat
      361 caaaaaagag aaattacttg aggcaaaaga agaaaaccaa atcctaagag aacaaactga
      421 agcagaacta cgagaaagac gtagcgaact tcaaagacaa gaaacccgac ttcttcaaaa
      481 agaagaaaac ttagagcgca aatctgatct attagataaa aaagatgaga ttttagagca
      541 aaaagaatca aaaattgaag aaaaacaaca acaagtagat gcaaaagaga gtagtgttca
      601 aacgttaata atgaagcatg aacaagaatt agaacgcatc tccggtctca ctcaagaaga
      661 agctattaat gagcaacttc aaagagtaga ggaagaactg tcacaagata ttgcagtact
      721 tgttaaagaa aaagaaaaag aagctaaaga aaaagttgat aaaacagcaa aagaattatt
      781 agctacagca gtacaaagat tagcagcaga tcacacaagt gaatcaacgg tatcagtagt
      841 taacttacct aatgatgaga tgaaaggtcg aatcattgga cgagaaggac gaaacatccg
      901 cacacttgaa actttaactg gcattgattt aattattgat gacacaccag aagcggttat
      961 attatctggt tttgatccaa taagaagaga aattgctaga acagcacttg ttaacttagt
     1021 atctgatgga cgtattcatc caggtagaat tgaagatatg gtcgaaaaag ctagaaaaga
     1081 agtagacgat attattagag aagcaggtga acaagctaca tttgaagtga acgcacataa
     1141 tatgcatcct gacttagtaa aaattgtagg gcgtttaaac tatcgtacga gttacggtca
     1201 aaatgtactt aaacattcaa ttgaagttgc gcatcttgct agtatgttag ctgctgagct
     1261 aggcgaagat gagacattag cgaaacgagc tggactttta catgatgttg gtaaagcaat
     1321 tgatcatgaa gtagaaggta gtcatgttga aatcggtgta gaattagcga aaaaatatgg
     1381 tgaaaatgaa acagttatta atgcaatcca ttctcaccat ggtgatgttg aacctacatc
     1441 tattatatct atccttgttg ctgctgcaga tgcattgtct gcggctcgtc caggtgcaag
     1501 aaaagaaaca ttagagaatt atattcgtcg attagaacgt ttagaaacgt tatcagaaag
     1561 ttatgatggt gtagaaaaag catttgcgat tcaggcaggt agagaaatcc gagtgattgt
     1621 atctcctgaa gaaattgatg atttaaaatc ttatcgattg gctagagata ttaaaaatca
     1681 gattgaagat gaattacaat atcctggtca tatcaaggtg acagttgttc gagagactag
     1741 agcagtagaa tatgcgaaat aatttttgtc tccctcacaa attagtgagg gagctttttt
     1801 aagttgtagt ctcaatctaa ttagacagca ctttatcggt aataactata gtaaacagtg
     1861 gttatttgaa agtaagacgg accttatatt aaataagaag ttattgcttt taataaaaat
     1921 gttttaggct
//
LOCUS       DAHHSC010000291         1928 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.296, whole genome shotgun sequence.
ACCESSION   DAHHSC010000291 DAHHSC010000000
VERSION     DAHHSC010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1928)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1928)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1928
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.296"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            98..811
                     /locus_tag="NBI57_002585"
     CDS             98..811
                     /locus_tag="NBI57_002585"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HCD1823504.1"
                     /translation="MAKLNVEVFADGADIEEMKAAYKNKQVDGFTTNPSLMAKAGVTD
                     YKAFAEEAVKEIPDASISFEVFADDLETMEKEAAILKQYGENVFVKIPIVNTKGESTI
                     PLIKKLSADNVRLNVTAVYTIEQVKEITEAVTEGVPTYVSVFAGRIADTGVDPLPLMK
                     EAVKVTHSKDGVKLLWASCRELFNVIQADEIGADIITCPADVVKKVNTNLGRDINELS
                     VDTVKGFAKDIQSSGLSIL"
     gene            complement(1251..1886)
                     /gene="sdpA"
                     /locus_tag="NBI57_002586"
     CDS             complement(1251..1886)
                     /gene="sdpA"
                     /locus_tag="NBI57_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001168910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease SdpA"
                     /protein_id="HCD1823505.1"
                     /translation="MQKFKDFFYDDLSVTRGNYFLTLMAAFFITIILFIGIVVSEVHL
                     LYSMLIVLVGLILLRLFKINLFSFKKLTLSQVIYIIGGALLIYGLDNLYLYFHDVPAN
                     ELQLEQEIRNTPFYISIFTVTIIPAIVEEIVFRGMIIRVIFRKHLFLGLLVSSLVFAS
                     LHESDTWIGYLPYLYSGLIFGLIYIKTKRLEVVIFMHFLNNLLALLFIIWG"
BASE COUNT          753 a          256 c          317 g          602 t
ORIGIN      
        1 taacaaagaa ataaaaatca ttttttacaa atggttgtaa gaaaaagaca tgcagatgtt
       61 gttaaaattt taataagaat caaggaggct atattatatg gctaaactaa atgtagaagt
      121 atttgcggac ggtgcagata ttgaagaaat gaaagcagct tataaaaaca aacaagtgga
      181 tggttttaca acaaatccta gtttaatggc gaaagcgggc gtaacagatt acaaagcttt
      241 tgctgaagaa gctgtgaaag aaattccaga tgcttcaatt tcatttgaag tatttgcaga
      301 cgatttagaa actatggaaa aagaagcagc aattttaaaa caatatggcg aaaatgtatt
      361 tgttaaaatt cctattgtaa atacaaaagg tgaatcaacg attcctttaa ttaaaaaact
      421 ttcagctgac aatgtgagat taaacgttac ggctgtttac acaattgaac aagttaaaga
      481 aataactgaa gcagtaactg aaggtgtgcc aacatatgtt tcagtatttg caggacgtat
      541 tgcagataca ggcgtagatc cattaccatt aatgaaagag gctgtaaaag ttacgcatag
      601 taaagacggc gttaaattat tatgggcaag ttgccgcgaa ttattcaatg tgattcaagc
      661 tgatgaaatt ggtgcagata ttattacatg ccctgcagat gttgtgaaaa aagtgaatac
      721 aaacttaggt cgcgatatta acgaattatc agtagataca gttaaaggct ttgcgaaaga
      781 tattcaatct tcaggtcttt ctattctata atttataaac atatttaaac cctcataggc
      841 attgtgctta tgggggtttt tccatcatag ttattaatga agaagaagtc aaaattcagc
      901 taagtaaaag cccgagttat aaaaaagtct aaattgattc aacaggagta gaaaaggaaa
      961 ttagtctcgc atattttaga aaatgaataa aaacaattac tatatgcaac tcaagaatta
     1021 aatattattt aagccacata cttattagag tacaaaatat acaagttgta cttatatcta
     1081 ggtatgtgac ttttgatttt aagcgtaaag caaaagtttt tatgtttgta ttagttctaa
     1141 attattttgc acttgatgac acgtattttt gagtttaagt ggtgactcaa gttcaataaa
     1201 ataaatatca gatagataat ttgaaaataa tatgaaaggt tatctcaaaa ttatccccat
     1261 attataaaga gcagagctaa caagttattt aagaagtgca tgaatattac cacttctaat
     1321 cgttttgttt ttatataaat tagaccaaaa atcaaaccag aatataagta aggtaaataa
     1381 ccaatccaag tgtcagattc gtgtaatgat gcaaaaacta aactagacac aagtaaccct
     1441 aaaaataagt gttttctgaa tataaccctt attatcatac cgcgaaaaac aatttcttcc
     1501 acaatagcgg ggatgatggt aacagtgaaa atagaaatat agaatggtgt atttcgtatt
     1561 tcttgctcta attgtagttc atttgccggt acgtcatgaa aatataaata aagattatct
     1621 aacccataaa ttaatagtgc accgcctata atataaataa cttgagacaa tgttaatttt
     1681 ttaaaagaga ataaattgat tttgaatagc ctcaatagaa ttaaacctac taatacaatt
     1741 agcatgctat aaagtaaatg tacttcactg acaactatgc cgataaataa aatgatagta
     1801 ataaaaaatg ctgccattaa agttaaaaaa taatttcctc gtgtaaccga taaatcatcg
     1861 taaaaaaagt ctttgaattt ttgcatgcta aatcctttct tttcattttt taaaattatt
     1921 ttgaatgc
//
LOCUS       DAHHSC010000292         1925 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.297, whole genome shotgun sequence.
ACCESSION   DAHHSC010000292 DAHHSC010000000
VERSION     DAHHSC010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1925)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1925)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1925
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.297"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            186..962
                     /gene="seg"
                     /locus_tag="NBI57_002587"
     CDS             186..962
                     /gene="seg"
                     /locus_tag="NBI57_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_031927817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphylococcal enterotoxin type G"
                     /protein_id="HCD1823506.1"
                     /translation="MKKLSTVIIILILEIVFHNMNYVNAQPDPKLDELNKVSDYKNNK
                     GTMGNVMNLYTSPPVEGRGVINSRQFLSHDLIFPIEYKSYNEVKTELENTELANNYKD
                     KKVDIFGVPYFYTCIIPKSEPDINQNFGGCCMYGGLTFNSSENERDKLITVQVTIDNR
                     QSLGFTITTNKNMVTIQELDYKARHWLTKEKKLYEFDGSAFESGYIKFTEKNNTSFWF
                     DLFPKKELVPFVPYKFLNIYGDNKVVDSKSIKMEVFLNTH"
     gene            complement(1514..>1925)
                     /locus_tag="NBI57_002588"
     CDS             complement(1514..>1925)
                     /locus_tag="NBI57_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1829 domain-containing protein"
                     /protein_id="HCD1823507.1"
                     /translation="DLTLTTKSNVINIFYEEVFEFLYDQKIRGLAQVSVSGESGLKYS
                     IDYIVSETKSQPEKLVNFTNNLDFNKITNEAYIYRDIKPNRPSRNKLEPIMLIIVNDV
                     DHPINERAQTVAEHENLEILKWSNKSKIIETLTS"
BASE COUNT          677 a          222 c          299 g          727 t
ORIGIN      
        1 gaatacggat tcaaaattat ttgttgtaat tagtaataat gataaataga attttaattt
       61 attctgctag tgaattttta gttttaaaac aatgctatcg acacactaca acctgaacta
      121 tctataagcg tgaatttata ataaggttca ttgtcaaata gactgaataa gttagaggag
      181 gttttatgaa gaaattatct actgtaatta ttattttgat tctagaaata gtttttcata
      241 atatgaatta tgtgaatgct caacccgatc ctaaattaga cgaactaaat aaagtaagtg
      301 attataaaaa taataaggga actatgggta atgtaatgaa tctttatacg tctccacctg
      361 ttgaaggaag aggagttatt aattctagac agtttttatc tcatgattta atttttccaa
      421 ttgagtataa gagttataat gaggttaaaa ctgaattaga aaatacagaa ttagctaaca
      481 attataaaga taaaaaagta gacatttttg gcgttccata tttttataca tgtataatac
      541 ctaaatctga accggatata aaccaaaatt ttggaggttg ttgtatgtat ggtggtctta
      601 catttaatag ttcagaaaat gaaagagata aattaattac tgtacaggta acaatcgaca
      661 atagacaatc acttggattt acaataacta caaataagaa tatggttact attcaggaac
      721 tagattacaa agcaagacac tggctcacta aagaaaaaaa gctatacgag tttgatggtt
      781 ctgcatttga atctggatat ataaaattta ctgaaaagaa caatacaagt ttttggtttg
      841 acttatttcc taaaaaagaa ctagtacctt ttgttccata taagttttta aatatttacg
      901 gagataataa agttgttgat tctaagagta ttaaaatgga agtatttctt aatactcact
      961 gataagtata tttgatatca ctatacaatc ataaagtgcg tttttcgaac ttttgttcta
     1021 agtgttttat agattcatgt aaatagttag tttattgccg gttaaattat ttaatttttc
     1081 taaatgattt attctaaata tattttatgt caatagcggt ttaaaaatgc tgttttatgg
     1141 cggtttgaaa atgacgtgct tttaaatgta tttttacttt tataatcttt tagtcgacat
     1201 gattctccag tggaaatgat aatttaataa cgtacattta tgattgttta agaatgtaca
     1261 attttttaac actctagagg taaatctatc taggtaatcc gaattctccg ttaatttaac
     1321 ggaaatacaa tgaattgtta gttttcttaa aagagcaagt gtcaaatacg tatcaagaaa
     1381 aagtttttct gactcgttga ccttgttctt tcttgttcta aaataaaacg tgcaaataaa
     1441 aaagttatct gcttttctat ttgcacgata tggtttggaa attaaagaat ataaataagg
     1501 gactactgct aatttatgag gttaaagttt caatgatttt tgatttatta gaccatttta
     1561 atatctcaag gttttcatgt tctgctacag tttgtgctct ttcatttatc ggatgatcta
     1621 catcatttac aattatcaac atgataggtt ctaatttgtt tctggatggt ctgtttggtt
     1681 tgatatcacg gtaaatataa gcttcattag ttattttgtt gaaatctaag ttgtttgtaa
     1741 agttaactag tttttctggt tgtgattttg tttcagaaac aatataatca atagaatatt
     1801 ttaaacctga ttctccagaa acagaaactt gtgctaaacc tcttattttt tggtcatata
     1861 agaattcaaa tacttcttca tagaatatgt ttataacgtt agatttagta gttaaagtaa
     1921 ggtca
//
LOCUS       DAHHSC010000293         1917 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.298, whole genome shotgun sequence.
ACCESSION   DAHHSC010000293 DAHHSC010000000
VERSION     DAHHSC010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1917)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1917)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1917
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.298"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            complement(<1..823)
                     /locus_tag="NBI57_002589"
     CDS             complement(<1..823)
                     /locus_tag="NBI57_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCD1823508.1"
                     /translation="MNLDWYYTFVILAKTLNYRLASEEINLTIPSIHKQIKNLEQHLN
                     VKLFETYKNQIILTEDGHTFLPIAQSFIEQYESGIKHIQLKKTMFQSKLNVVVSSYIA
                     TFIMPKFLKSFFNEHPFIDVSLHVKNENIEKDINNHTYDIGISRNQPKLREVHSEKVC
                     EGKIVLIAPNKENNHLLTEASLFEKYKIISDNHPEYWSSLKNNILNIYGKAQFLSIND
                     VHTSIKLIEMNQGISFLPIYITTDSDYNISVINTKILQAPISFTYIYSKKESPEIL"
     gene            933..>1917
                     /locus_tag="NBI57_002590"
     CDS             933..>1917
                     /locus_tag="NBI57_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HCD1823509.1"
                     /translation="MIRIQDNTIRDGMQQSNVRKSLIIKKEVLKQINKLNINSVEVGM
                     CTTIEDEFNIHQFRDILSPEKELVVLTRLNEKEIKKIVKLKIHNLVVKILLPISDLHI
                     KEKLNFSNKYYIQKIKDCLDILKKDKKGVDICFEDATRTSREKLKEYMEIISKYQVRT
                     VTFADTVGCSTPLEYGDIFNYFVKKYSNIIFSAHCHNDLGLATANTLAAILNGAKQIE
                     TTFLGIGERAGNAPIEEIITILTKKQIESTEFTLPDVYKTSINISKILDFQISENKPI
                     IGENIFKHESGIHQDGTKKNINMYQYLVPSDLGLKNSQVVQFPISNISSKKI"
BASE COUNT          727 a          227 c          293 g          670 t
ORIGIN      
        1 ccaatatttc tgggctttct tttttgctgt atatatatgt aaatgaaata ggcgcttgta
       61 aaattttcgt gttaataacc gaaatattgt aatcactatc tgttgttata taaataggca
      121 ggaatgatat accttgattc atttcgatta atttaattga agtatgcaca tcattgatag
      181 atagaaattg tgcttttcca taaatattta aaatattatt tttaagtgat gaccaatatt
      241 ctggatggtt atcacttatt attttgtatt tttcaaataa agatgcctca gttagaagat
      301 gattattctc tttattggga gcaatcaata caattttacc ttcgcatact ttttcagaat
      361 gaacttctct tagtttaggt tgatttctgc taatcccgat gtcatacgta tgattattaa
      421 tatctttttc aatattttca tttttgacat ggagagaaac atcgataaac ggatgctcgt
      481 taaagaaaga ttttaaaaat ttgggcataa tgaatgtcgc gatatatgaa gacacgacaa
      541 catttaattt cgattgaaac atcgtttttt taagttggat atgtttgatg ccactttcat
      601 attgttcaat aaaactttgc gcaattggaa gaaatgtatg accatcttca gtcaagataa
      661 tttgattttt ataagtttca aatagtttca cattgagatg ctgttctaaa tttttaattt
      721 gcttatgtat agaaggtata gtgagattaa tttcttcact agctaatcga tagtttaacg
      781 tcttcgctaa aatgacaaat gtatagtacc aatccaaatt catgaactta cctctttcaa
      841 aaaagttaat aattcattat ataatattta atttttattg cataaaaata atattaatat
      901 tatatcagag gttatcgagg gggtgaagta atatgattcg aattcaagat aatacaataa
      961 gggatggtat gcaacaaagt aatgttcgaa aaagtctaat tataaaaaaa gaagtattga
     1021 aacaaattaa caagttaaat ataaattctg ttgaagtagg catgtgtaca actatcgagg
     1081 atgaatttaa tattcatcaa ttcagagaca ttttaagtcc tgaaaaagaa ttagtagtat
     1141 tgactaggct taatgaaaaa gaaataaaaa aaatagtcaa attgaaaatt cataatttag
     1201 tggtaaaaat actattgcca atatctgact tgcatataaa agaaaagctt aatttttcaa
     1261 ataaatatta tattcagaaa atcaaagact gcttggatat attaaagaaa gataaaaaag
     1321 gagtagatat ttgttttgaa gatgcaacaa ggacttctag agaaaaattg aaagaataca
     1381 tggaaattat ttcaaaatat caagttagaa cagttacatt tgcggacact gtaggatgtt
     1441 cgacaccatt agaatacgga gatattttta attactttgt aaaaaaatat tctaacataa
     1501 ttttttctgc tcattgtcat aacgatctag ggttggctac tgcaaataca ttagctgcaa
     1561 ttttaaatgg tgcaaagcaa atagaaacta catttttggg aattggtgag agagcgggta
     1621 atgctcctat tgaggaaata attactattt tgacaaaaaa acaaatagaa agtacggaat
     1681 tcactttacc cgacgtatat aaaactagta ttaatatttc taaaattctc gattttcaaa
     1741 tatcagaaaa caaacctata attggtgaaa atatatttaa acatgaatca ggaattcatc
     1801 aagatggtac taaaaaaaat ataaatatgt atcaatattt agttcctagt gatttaggat
     1861 tgaaaaattc acaagttgtt cagtttccaa taagtaatat ttctagtaag aaaatct
//
LOCUS       DAHHSC010000294         1904 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.299, whole genome shotgun sequence.
ACCESSION   DAHHSC010000294 DAHHSC010000000
VERSION     DAHHSC010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1904)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1904)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1904
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.299"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            complement(4..1170)
                     /gene="norA"
                     /locus_tag="NBI57_002591"
     CDS             complement(4..1170)
                     /gene="norA"
                     /locus_tag="NBI57_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter NorA"
                     /protein_id="HCD1823510.1"
                     /translation="MNKQIFVLYFNIFLIFLGIGLVIPVLPVYLKDLGLTGSDLGLLV
                     AAFALSQMIISPFGGTLADKLGKKLIICIGLILFSVSEFMFAVGHNFSVLMLSRVIGG
                     MSAGMVMPGVTGLIADISPSHQKAKNFGYMSAIINSGFILGPGIGGFMAEVSHRMPFY
                     FAGALGILAFIMSIVLIHDPKKSTTSGFQKLEPQLLTKINWKVFITPVILTLVLSFGL
                     SAFETLYSLYTADKVNYSPKDISIAITGGGIFGALFQIYFFDKFMKYFSELTFIAWSL
                     LYSVVVLILLVFANGYWSIMLISFVVFIGFDMIRPAITNYFSNIAGERQGFAGGLNST
                     FTSMGNFIGPLIAGALFDVHIEAPIYMAIGVSLAGVVIVLIEKQHRAKLKEQNM"
     gene            1379..1801
                     /locus_tag="NBI57_002592"
     CDS             1379..1801
                     /locus_tag="NBI57_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HCD1823511.1"
                     /translation="MKLQKSNHTILLVLEKGEDIVECITTFADDQDLTFTSVSGIGAC
                     DDVVLKFFNLTTKQYEEKHITEPLELTSLLGNISRLDNGHFAHLHATFGTQSYETFSG
                     HLAKAIVSATAEIILTVTDLDIQRSFKDAVGLNLLDPQ"
BASE COUNT          682 a          354 c          263 g          605 t
ORIGIN      
        1 atgctacata ttttgttctt tcaattttgc tctatgttgc ttttcaatta aaacaataac
       61 aacacctgct aatgaaacac ctatagccat ataaattggt gcttcaatgt gtacatcaaa
      121 taacgcacct gcgattaaag gacctatgaa attacccata ctagtgaatg tcgagttcaa
      181 tccgcctgca aagccttgcc tttctccagc aatattagaa aaataatttg taatggctgg
      241 tcgtatcata tcaaaaccta tgaagacaac aaaactgatt aacattattg accaatagcc
      301 attagcaaaa actaataata ttaagacaac aactgaatat aataatgacc aagctataaa
      361 tgttaactct gagaaatact tcataaattt atcgaagaaa tagatttgga aaagtgcccc
      421 aaatataccg ccacccgtaa tagcaatcga aatatcttta ggtgaataat ttaccttgtc
      481 agctgtgtat agtgaataca atgtttcaaa tgcagataaa ccaaacgata atacaagtgt
      541 taaaataact ggtgtaataa acactttcca gttaattttc gttagcaatt gtggctctaa
      601 cttttggaaa ccacttgtcg tagacttttt cggatcgtga atcaatacaa ttgacattat
      661 aaatgctaga atacctaatg ctcctgcaaa gtaaaatggc atacgatgtg aaacttctgc
      721 cataaatcca ccaatccctg gtcctaaaat gaatccagaa ttgataatcg ctgacatgta
      781 gccaaagttt tttgcttttt gatggcttgg tgaaatgtca gctattaaac ctgtcacacc
      841 aggcattacc ataccagcac tcataccacc aatcactctc gataacatca ataccgaaaa
      901 attgtggcca actgcaaaca taaattctga cactgaaaac aaaattaatc ctatacatat
      961 aattaatttc ttccctaatt tgtcagctag cgtaccacca aacggcgata taatcatttg
     1021 agataacgca aaagcagcaa ctagtaatcc taaatcacta ccagttaatc ccaaatcttt
     1081 taaataaaca ggcaagactg gtattactaa accgatacct aaaaaaatca agaaaatatt
     1141 aaaatataag acaaaaatct gtttattcat atgctcacct cttttttctc catgtcatgc
     1201 ttaaagctgt tattcttctt ttccacgaca gattgcaatt catgctaaat attcgtaaaa
     1261 agtttctata ttgtattaca acattgctac atttgacaat attttttctt tgtaattatc
     1321 actattttcc atttaattgt ataattaatg acatattaaa ataaagggga ttgatcatat
     1381 gaaattgcaa aaaagtaatc atactatact actagtgttg gaaaaaggag aagatatcgt
     1441 tgaatgtata acaacctttg cagatgacca agacttaaca tttacatctg tcagcggcat
     1501 tggtgcatgt gatgacgttg tattaaaatt tttcaattta acaacgaaac aatacgaaga
     1561 aaaacatatt actgagccac tggaattgac gagcttatta ggtaatattt ctcgtctaga
     1621 caatggtcat tttgcacatt tgcatgctac tttcggtact caatcatatg aaacttttag
     1681 cggacatctc gctaaagcaa ttgtttctgc aactgctgaa atcattctta ctgtaactga
     1741 tttggacatt caacgttcat tcaaagatgc agtcggttta aatttacttg atcctcaata
     1801 gcactttact ttttagttga atcaaatttt attacaagtt atcataagtg cttaatcata
     1861 aatgcattct cgtttattaa tatacttcaa cgagttaaat taaa
//
LOCUS       DAHHSC010000295         1874 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.300, whole genome shotgun sequence.
ACCESSION   DAHHSC010000295 DAHHSC010000000
VERSION     DAHHSC010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1874)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1874)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1874
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.300"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            <1..>1874
                     /locus_tag="NBI57_002593"
     CDS             <1..>1874
                     /locus_tag="NBI57_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HCD1823512.1"
                     /translation="KVLSTIIDELQKCETFYFSVAFITESGLASLKAQLLDLSNKGVK
                     GKILTSNYLGFNSPKMYGELLKLKNVEVRLTDIAGFHAKGYIFEHKDYSSMVIGSSNL
                     TSNALKVNYEHNVLLSTMKNGDLVDSVKNEFELLWQKSTPLTEQWINSYKESFEYRSL
                     EKLAEVEQTQMLLADKVKKSVEIVPNLMQAEALRSLKAIRDKTKDKALIISATGTGKT
                     ILCALDVREVNPNKFLFIVHNEGILNRAKEEFKKVLPIKNDSDFGLLTGKHRDVDAKY
                     LFATIQTLSRDDNFKQFDENEFDYIVFDEAHRSAASTYQRVFNYFKPKFMLGMTATPE
                     RSDELSIFELFDYNIAYEIRLQAALESDILCPFHYFGVTDYVHQGIKEDDVTKLRYLT
                     SDERVNYIIQKTDYYGYSGEILQGLIFVSSKKEAYDLADKLSSKGIKSVALTGDDSVN
                     YRQIVIEKLKEGKINYIITVDLFNEGIDIPEVNQVVMLRPTESSIIFIQQLGRGLRKS
                     ANKEYVTVIDFIGNYKTNYLIPIALSGDQSQNKDNYKKFLTNNDSINGVSTINFEEVA
                     KKQIYNSLDAVSLNQNKLILKAYEEVENRLGHMPLLMDFIQQHSIDPSVIFSKFSNYY
                     "
BASE COUNT          718 a          208 c          334 g          614 t
ORIGIN      
        1 aaaaagtcct ttcaacaatt atagatgaac ttcaaaaatg tgaaacgttc tatttttctg
       61 tagcttttat aacagaaagc ggattagcca gcttaaaggc tcaactatta gatttaagca
      121 ataagggtgt taaagggaaa atattaacat ctaattactt aggatttaat agtcctaaaa
      181 tgtatggaga attacttaaa ttaaaaaatg tagaggtaag attaactgat attgctggat
      241 tccatgccaa aggatatatt tttgaacata aggattatag ttctatggta ataggaagct
      301 ctaatttaac atctaatgcg ttaaaggtta attatgagca taatgtttta ctgtctacta
      361 tgaaaaatgg tgatttagtt gatagtgtaa aaaatgaatt tgaattgtta tggcaaaaga
      421 gtaccccact gactgagcaa tggattaatt catataaaga atcatttgag taccgttcct
      481 tagaaaaatt agcggaggtt gaacaaactc aaatgctgct agctgataag gtgaaaaaat
      541 cagtggaaat tgtacccaat ttaatgcaag cagaagcatt aaggtcatta aaagctatta
      601 gggataaaac caaagataag gcgttaataa tatctgcaac tggtacaggt aaaacgattt
      661 tatgtgcatt agatgttaga gaagtaaacc ctaataaatt tttatttatt gttcataatg
      721 aggggatttt aaatagagct aaggaagaat ttaaaaaagt attacctata aaaaatgata
      781 gtgattttgg attgttgaca ggaaaacata gagatgtcga tgccaaatat ttatttgcaa
      841 ccattcaaac attatctaga gatgataatt ttaagcaatt tgatgaaaat gaatttgatt
      901 atattgtttt tgatgaagca catcgttctg ctgcatctac ttatcaacga gtatttaact
      961 acttcaaacc taagttcatg ctgggaatga ctgctacacc agaaagatca gatgaattaa
     1021 gtatatttga actgtttgat tataatattg catatgaaat aaggttacaa gcagcgttag
     1081 aaagtgatat tttatgtcca tttcattatt ttggtgtgac tgattatgta catcaaggta
     1141 ttaaagaaga tgatgtaact aaactgagat atttaacttc tgatgaaaga gttaattata
     1201 ttattcaaaa gacagattac tatggatatt caggtgaaat tttacaagga ttaatttttg
     1261 tgagtagcaa aaaagaagct tatgatttag cggataaact aagttcaaaa gggattaaaa
     1321 gtgttgcttt aacaggagat gatagtgtta attatcgcca aattgtaata gaaaaattaa
     1381 aagaaggtaa gattaactat attataactg tagatctttt taacgaaggt attgatattc
     1441 cagaagtcaa tcaagttgta atgttgagac caacagagtc tagtattata ttcattcagc
     1501 aacttggtag aggattaaga aaaagtgcaa ataaagagta tgttactgta attgatttta
     1561 ttggtaatta taagaccaat tatttaattc cgattgcgct ttctggggat caatcgcaaa
     1621 ataaagataa ttataagaag tttttaacga ataacgattc gattaatgga gtatctacaa
     1681 ttaattttga agaagttgct aaaaaacaga tttacaattc attggatgca gtatcattga
     1741 atcaaaataa attaatatta aaagcttatg aagaagttga aaatagattg ggacacatgc
     1801 cgttactaat ggattttata caacaacatt ctatagatcc aagcgttata ttttctaaat
     1861 ttagtaatta ttac
//
LOCUS       DAHHSC010000296         1851 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.301, whole genome shotgun sequence.
ACCESSION   DAHHSC010000296 DAHHSC010000000
VERSION     DAHHSC010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1851)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1851)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1851
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.301"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            284..1804
                     /locus_tag="NBI57_002594"
     CDS             284..1804
                     /locus_tag="NBI57_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HCD1823513.1"
                     /translation="MQNFKELGISDNTVQSLESMGFKEPTPIQKDSIPYALQGIDILG
                     QAQTGTGKTGAFGIPLIEKVVGKQGVQSLILAPTRELAMQVAEQLREFSRGQGVQVVT
                     VFGGMPIERQIKALKKGPQIVVGTPGRVIDHLNRRTLKTDGIHTLILDEADEMMNMGF
                     IDDMRFIMDKIPAVQRQTMLFSATMPKAIQALVQQFMKSPKIIKTMNNEMSDPQIEEF
                     YTIVKELEKFDTFTNFLDVHQPELAIVFGRTKRRVDELTSALISKGYKAEGLHGDITQ
                     AKRLEVLKKFKNDQINILVATDVAARGLDISGVSHVYNFDIPQDTESYTHRIGRTGRA
                     GKEGIAVTFVNPIEMDYIRQIEDANGRKMSALRPPHRKEVLQAREDDIKEKVENWMSK
                     ESESRLKRISTELLNEYNDVDLVAALLQELVEANDEVEVQLTFEKPLSRKGRNGKPSG
                     SRNRNSKRGNPKFDSKSKRSKGYSSKKKSTKKFDRKEKSSGGSRPMKGRTFADHQK"
BASE COUNT          677 a          274 c          364 g          536 t
ORIGIN      
        1 catgtgtttc agtaaaattt gttgtggaat gttaacgata tacgtatttt ataaaaaatt
       61 ttttataatg attattcgaa tgatgcgtaa cgcttacatc ttatctaatg ctagcttttt
      121 gacaaaaata tgacaatcaa ttaatgtgat tctaataaat attcgcaaat tgctttattg
      181 cgattaaatt tttttggtgg tactatatag aagttgatga aatattaatg aacttatatg
      241 caaaagtata ttgagaaata aacaggtaaa aaggagaatt attttgcaaa attttaaaga
      301 actagggatt tcggataata cggttcagtc acttgaatca atgggattta aagagccgac
      361 acctatccaa aaagacagta tcccttatgc gttacaagga attgatatcc ttgggcaagc
      421 tcaaaccggt acaggtaaaa caggagcatt cggtattcct ttaattgaga aagtagtagg
      481 gaaacaaggg gttcaatcgt tgattttagc acctacaaga gaattggcaa tgcaggtagc
      541 tgaacaatta agagaattta gccgtggaca aggtgtccaa gttgttactg tattcggtgg
      601 tatgcctatc gaacgccaaa ttaaagcctt gaaaaaaggc ccacaaatcg tagtcggaac
      661 acctgggcgt gttatcgacc atttaaatcg tcgcacatta aaaacggacg gaattcatac
      721 tttgatttta gatgaagctg atgaaatgat gaatatggga ttcatcgatg atatgagatt
      781 tattatggat aaaattccag cagtacaacg tcaaacaatg ttgttctcag ctacaatgcc
      841 taaagcaatc caagctttag tacaacaatt tatgaaatca ccaaaaatca ttaagacaat
      901 gaataatgaa atgtctgatc cacaaatcga agaattctat acaattgtta aagaattaga
      961 gaaatttgat acatttacaa atttcctaga tgttcatcaa cctgaattag caatcgtatt
     1021 cggacgtaca aaacgtcgtg ttgatgaatt aacaagtgct ttgatttcta aaggatataa
     1081 agctgaaggc ttacatggtg atattacaca agcgaaacgt ttagaagtat taaagaaatt
     1141 taaaaatgac caaattaata ttttagtcgc tactgatgta gcagcaagag gactagatat
     1201 ttctggtgtg agtcatgttt ataactttga tatacctcaa gatactgaaa gctatacaca
     1261 ccgtattggt cgtacgggtc gtgctggtaa agaaggtatc gctgtaacgt ttgttaatcc
     1321 aatcgaaatg gattatatca gacaaattga agatgcaaac ggtagaaaaa tgagtgcact
     1381 tcgtccacct catcgtaaag aagtacttca agcacgtgaa gatgacatca aagaaaaagt
     1441 tgaaaactgg atgtctaaag agtcagaatc acgcttgaaa cgcatttcta cagagttgtt
     1501 aaatgaatat aacgatgttg atttagttgc tgcactttta caagagttag tagaagcgaa
     1561 cgatgaagtt gaagttcaat taacttttga aaaaccatta tctcgcaaag gccgtaatgg
     1621 taaaccaagc ggttctcgta acagaaatag taagcgtggt aatcctaaat ttgacagtaa
     1681 gagtaaacgt tcaaaaggat actcaagtaa gaagaaaagt acaaaaaaat tcgaccgtaa
     1741 agagaagagc agcggtggaa gcagacctat gaaaggtcgc acatttgctg accatcaaaa
     1801 ataatttata gattaagagc ttaaagatgt aatgtcttga gctctttttt g
//
LOCUS       DAHHSC010000297         1851 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.302, whole genome shotgun sequence.
ACCESSION   DAHHSC010000297 DAHHSC010000000
VERSION     DAHHSC010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1851)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1851)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1851
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.302"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            complement(194..1123)
                     /locus_tag="NBI57_002595"
     CDS             complement(194..1123)
                     /locus_tag="NBI57_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001574632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HCD1823514.1"
                     /translation="MLLFYIIGVLVGMLIPIQTSVNSRLSLYTKSPFYTSFISFSVGT
                     ICLIILNIIINPEVFTIHFYNNQSFNYTWVVGGLLGVSFLTGNLLLLPKLGATLTVIA
                     TVAGQIIMGVIIDTFGLFGATIHDFNLIKAIGVLLLIVGIVIMNQFNKNNLLLTDQKY
                     LLFWLLLGFIFGFFPPIQTTINSALASHTHSPAFASLVSFTIGSITLLILTAIFNSSL
                     KLKTSHLKFGKLKPIYFTGGILGMAFVTANIILMPHMGAALTTLIGMFGQILMGILID
                     HFGLFGSPKIAMTSRKTIGLLCILTGIILLRLF"
     gene            complement(1318..1542)
                     /locus_tag="NBI57_002596"
     CDS             complement(1318..1542)
                     /locus_tag="NBI57_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499185.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2922 domain-containing protein"
                     /protein_id="HCD1823515.1"
                     /translation="MTKTLELSFKSSLDKPVKLQLPQLNNVVTEQVARDSMNALVDLN
                     IIKSNSGTITKVHSAQIIDKTTTVLFEDKK"
     gene            complement(1559..1762)
                     /locus_tag="NBI57_002597"
     CDS             complement(1559..1762)
                     /locus_tag="NBI57_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001837138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1823516.1"
                     /translation="MSKINHITIVLSFTKVDANGKQTEFKRRFANINPDASNDQIKTF
                     SKLIERLTGETYNNIELIKSLSI"
BASE COUNT          693 a          260 c          276 g          622 t
ORIGIN      
        1 gtgtaaatta ttaaaaaata acattaattt aggtcttaag aatgagttga ctatcgaatt
       61 tgctaaatta aaatttgttg tgtcaaagta tactacccaa atcaacgctt ttctaaagat
      121 ttttagacca caaaaaaata gcttatcact tttaaaaata attacaagtt aaaatgataa
      181 gctaaaagtt aatttaaaat aatcttaaaa gtataatgcc tgtcaaaata cataatagac
      241 caatagtttt tctggatgtc attgctattt taggtgaacc aaataatcca aagtgatcta
      301 tcaatatgcc cattagaatc tggccaaaca tcccaataag tgttgttaat gctgcaccca
      361 tatgaggcat taagataatg ttagctgtta caaaagccat accaagtata ccgccagtaa
      421 aatagatagg ctttaattta ccgaatttta aatgacttgt ttttagtttt aaagaactat
      481 taaaaatagc ggttaaaatc aatagcgtta ttgacccaat tgtaaatgat actaatgatg
      541 caaaggctgg tgaatgagta tgacttgcta aagcactatt aattgtcgtt tgaataggtg
      601 gaaagaaacc aaaaataaat cctaagagaa gccaaaacag taaatacttt tgatcagtta
      661 gtaataaatt attcttgtta aattgattca ttatgacgat gccgacaatg agtaacaata
      721 ctccaattgc tttaattaaa ttaaaatcat gaattgtagc gccaaataag ccaaatgtat
      781 caataatgac acccataata atttgacccg caactgttgc aattacagtt aatgttgcac
      841 ctaattttgg caataacaat aaattgccag ttaaaaagct aaccccaagc aaaccaccga
      901 ctacccatgt gtagttaaat gattgattat tgtaaaagtg aatagtaaat acttctggat
      961 tgataatgat atttaaaata attaaacaaa ttgttccaac tgaaaatgaa atgaatgaag
     1021 tatagaaagg cgatttagta tacagtgata gtcttgaatt gacagatgtt tggataggaa
     1081 taagcattcc aactaggaca ccaatgatat agaaaagaag catatgaata aagtctcact
     1141 ttccaaagtt aataataaaa tcatatgaat acattatcat tatagagaaa atatacaact
     1201 catttacaat aatgttgaaa aaaacaggac attatcataa caaaatcacc ctagacaatc
     1261 atgatgaaag tctagggtgt tagtatttat actaaattaa ttgcgctttc tttatatcta
     1321 tttcttatct tcaaataaaa cagttgttgt tttatcaata atttgagcag aatgtacttt
     1381 ggtgatagta ccactatttg actttataat atttaaatct actaatgcat tcatactatc
     1441 acgtgcaact tgctcggtca ctacgttgtt taattgtggt aattgcagtt ttacaggctt
     1501 gtctagtgaa gatttaaaac ttaattcaag tgttttagtc atgaatatgt cctcctgatt
     1561 aaattgataa agatttgatg agttcgatat tgttatatgt ttctccagta agtcgctcaa
     1621 taagtttgct gaatgtttta atttggtcgt ttgatgcatc agggttaatg ttagcgaatc
     1681 gacgcttaaa ttctgtttgt ttgccgttag cgtctacttt agtaaatgat aatacaatag
     1741 tgatgtggtt tattttactc atattttaaa acctcctttc acactatata tcgaaataaa
     1801 ataataaaat ggctaatttt attttctatg tttaaaatct ataaaaaagg c
//
LOCUS       DAHHSC010000298         1838 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.303, whole genome shotgun sequence.
ACCESSION   DAHHSC010000298 DAHHSC010000000
VERSION     DAHHSC010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1838)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1838)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1838
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.303"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            32..820
                     /locus_tag="NBI57_002598"
     CDS             32..820
                     /locus_tag="NBI57_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCD1823517.1"
                     /translation="MNKVTINPQIQLTYQIEGKGDPIILLHGLDGNLAGFEDLQHQLA
                     SSYKVLTYDLRGHGKSSKSESYDLNDHVEDLKILMEKLNIHEAHILGHDLGGVVAKLF
                     TDKYAYRVKSLTTIASKKDDLIHSFTQLLIQYQDDIAGFNKSEAYILLFSKLFRNQEK
                     TMKWYQKQRIYSIKSEDDSAVAIRSLILHKDEPMYLKKRTCVPTLLINGEHDPLIKDK
                     NHFKLEAHFLNVTKKIFEHSGHAPHIEEPEAFMNYYLNFLKSVS"
     gene            complement(1072..1446)
                     /locus_tag="NBI57_002599"
     CDS             complement(1072..1446)
                     /locus_tag="NBI57_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SarA family transcriptional regulator"
                     /protein_id="HCD1823518.1"
                     /translation="MAITKINDCFELLSMVTYADKLKSLIKKEFSISFEEFAVLTYIS
                     ENKEKEYYLKDIINHLNYKQPQVVKAVKILSQEDYFDKKRNEHDERTVLILVNAQQRK
                     KIESLLSRVNKRITEANNEIEL"
BASE COUNT          646 a          240 c          281 g          671 t
ORIGIN      
        1 gggtatatag tttattgagg gaggtgtcac aatgaataaa gtcacaatta atcctcaaat
       61 ccaattaact tatcaaattg aaggtaaagg ggatcctata atattacttc atggattgga
      121 tggtaattta gctggatttg aagatttgca acatcaacta gcatcatcat ataaagtact
      181 tacttacgat ttaagaggtc atggcaagtc ttctaaaagt gaatcatacg atttaaacga
      241 tcacgttgag gatttaaaaa ttctaatgga gaagttaaat attcatgagg cacatattct
      301 aggacatgat ttaggtgggg tagttgctaa gttatttaca gataaatatg cttatcgtgt
      361 aaaatcatta actaccattg catcgaagaa agatgactta atacacagct ttactcaatt
      421 gttaatacaa tatcaagatg atatagcggg ttttaataag tctgaagcgt atattctttt
      481 attttctaaa ttgtttagaa atcaagagaa gacgatgaaa tggtatcaaa aacaaagaat
      541 atatagcatt aagtctgagg atgatagtgc ggtggcaatt cgttcattaa ttttgcataa
      601 agatgaacct atgtatttaa aaaaacgtac atgtgtacct actttgttaa ttaatgggga
      661 acatgatcct ttgattaaag ataagaatca ttttaaattg gaagcgcatt ttttaaatgt
      721 tacgaaaaaa atcttcgaac attcaggaca tgcaccgcat attgaagaac cagaagcatt
      781 tatgaattat tatttaaatt ttttaaaaag cgtatcataa tatgtgatat ataaacctag
      841 ggcataaagt ccttaggcaa tgtgaaaaag ctgattacta ttcattattt gatagaaatc
      901 agcttttttt gaaatgtatt tgatatatac tgctcgttat gcggctatct tccttatatt
      961 aagtgccatt agtgcaaaac ctcttaacaa ttaggtaaaa aaagacataa aaaaaggaag
     1021 tttaatagaa tgtatcatct atcaaacttc accaaattgc gctaaacaaa actatagttc
     1081 aatttcgttg tttgcttcag tgattcgttt atttactcga ctcaataatg attcgatttt
     1141 tttacgttgt tgtgcattaa caagaattaa tacagttctt tcatcatgct cattacgttt
     1201 tttatcgaag taatcttctt gagataaaat tttaactgct ttaacaactt gtggttgttt
     1261 gtagtttaaa tgattaataa tatctttaag atagtattct ttctctttgt tttcgctgat
     1321 gtatgtcaat acagcgaatt cttcaaagct aattgaaaat tcctttttaa ttaaactttt
     1381 taatttgtca gcataagtga ccattgataa caactcaaag caatcattga tttttgtaat
     1441 tgccatgttt aaaacctccc tatttgatgc atcttgctcg atacatttgc ccgataatat
     1501 attgatatct aatctttatt tattatagat atgttagtca taattttgca ttaaataagt
     1561 tttattaaat atatttaatg ctctattatt tagttaatta taactaatta aaaatgagaa
     1621 gtaaacaaaa aagtgtttat aaaacaaatt attcattaga cacagtgatt gtatttctgg
     1681 gttagcattt ggtttagtca aaaatatcag cattttttat aatttacctt aatttaatcg
     1741 ccaatacaag gtaatttatt taatgaaacg atttagcgca attaaatgtt tcgatattca
     1801 atgtgtgtct aaagagattc tttgttatag cttaactc
//
LOCUS       DAHHSC010000299         1826 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.304, whole genome shotgun sequence.
ACCESSION   DAHHSC010000299 DAHHSC010000000
VERSION     DAHHSC010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1826)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1826)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1826
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.304"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            <1..902
                     /locus_tag="NBI57_002600"
     CDS             <1..902
                     /locus_tag="NBI57_002600"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HCD1823519.1"
                     /translation="ESYFLFYINRPSIIVGKNQNTIEEVNQTYIDAHNIDVVRRISGG
                     GAVYHDTGNLNFSFITDDDGNSFHNFQKFTEPIVQALQSLGVNAELTGRNDIQVGQAK
                     ISGNAMVKVKNRMFSHGTLMLNSDLDEVQNALKVNPAKIKSKGIKSVRKRVANIQEFL
                     NDPLEIEEFKKIILKTIFGETEVEEYKLTDEDWENIEKLSNDKYRTWAWNYGRNPKYN
                     FEREEKFEKGFVQIKFDVKRGKIEHAKIFGDFFGVGDVTDLENALVGCLHDFEHIEEA
                     LSEYDLYHYFGDIDRHELIRLMS"
     gene            complement(983..1201)
                     /locus_tag="NBI57_002601"
     CDS             complement(983..1201)
                     /locus_tag="NBI57_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IDEAL domain-containing protein"
                     /protein_id="HCD1823520.1"
                     /translation="MKYNTNVKHTTLEAFVTTVNDLGIELIINEALREVRKRQLIELI
                     DDALVNKDEAAFNQYTAEYKNLEAFLGE"
     gene            1411..>1826
                     /locus_tag="NBI57_002602"
     CDS             1411..>1826
                     /locus_tag="NBI57_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001619687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComK"
                     /protein_id="HCD1823521.1"
                     /translation="MYSQNIYVIRKGDMVIRPAFDDDDQRNGSEIIRFDKTRIQNPFK
                     VQKIIERSCKFYGNTYLGKKAETNRITGISSKPPILLTPLFPTYFFPTHSDRQNENIW
                     LNMHYIESIKELKNRKCKVTFINNESIILHVSYHSL"
BASE COUNT          667 a          250 c          295 g          614 t
ORIGIN      
        1 aagaaagtta ctttttattt tacataaata gaccatctat cattgttgga aagaatcaaa
       61 atacgataga ggaagtaaat cagacatata tagatgctca taacatcgat gtagttagaa
      121 gaatttctgg tggtggcgct gtttatcatg atactggcaa tttaaacttt agttttataa
      181 cagatgatga tggtaacagt ttccacaatt tccaaaaatt cactgaacca attgttcaag
      241 cattacaatc tttaggcgta aatgctgaat taactggtcg taacgatatt caagttggac
      301 aagcgaaaat ctcagggaat gctatggtta aagttaaaaa tagaatgttt agtcatggta
      361 cgttgatgtt aaatagtgat ttagacgaag ttcagaatgc actaaaagtt aatccagcta
      421 agattaaatc aaaaggtatt aaatctgttc gtaaacgagt agcaaacatt caagaatttt
      481 taaatgaccc tttagaaatt gaagaattta aaaaaatcat cttgaaaact atctttggag
      541 aaactgaagt agaagaatat aaattaacgg atgaagattg ggaaaatatt gaaaagttaa
      601 gtaacgacaa atatagaact tgggcatgga attatggtag aaaccctaaa tataactttg
      661 aacgcgagga aaaatttgaa aaaggatttg tacaaattaa gtttgatgtt aaacgaggta
      721 aaatcgaaca tgcaaaaatt ttcggtgatt tctttggtgt cggagatgtc actgatcttg
      781 aaaatgcatt agttggctgt ctacacgact ttgagcacat agaagaagca ctatcagaat
      841 atgatttata tcattatttc ggtgacatag atagacatga attaattaga ttaatgtcat
      901 aaacgattaa aggactattc ttacatgtat gagatgttaa gaatagtcct tttatgattg
      961 cgttatggaa ttcgaatcaa tgttattcac cgagaaatgc ctccaaattt ttgtattctg
     1021 ccgtatattg attaaatgct gcttcatctt tattgacgag tgcgtcatct ataagttcta
     1081 tgagctgtcg ttttcttacc tctcgaagtg cttcattgat aattaattca atacccaaat
     1141 cattgacagt tgtgacaaac gcttctaaag ttgtatgttt aacattagta ttgtatttca
     1201 tagaaaccac tacctttctt taatttataa taagtataca tttttctaat tttgaaatca
     1261 atgaatattc tgactattag ttaaaaatta ttgtacattt tcaccaaaat aataacttga
     1321 caactcagaa ttttattata aaatatcttt atatcattaa ttgtattttt atattaatgg
     1381 tttaaaactt aataaagcga ggttttgctt atgtattctc aaaatattta tgtgatacgc
     1441 aaaggagaca tggttattcg accagcattt gatgatgacg atcaaagaaa cggtagtgaa
     1501 ataattcggt ttgacaaaac gcgtattcaa aatcctttta aagtccagaa aatcattgaa
     1561 cgctcttgca aattttatgg taatacttat cttggcaaga aagcagagac aaaccgcatt
     1621 actggcattt ctagtaaacc acctatttta ctaacaccat tatttccaac ttattttttc
     1681 ccaacacatt ctgacagaca aaatgaaaat atttggttaa atatgcatta tatcgaaagt
     1741 attaaagaat taaaaaatcg taaatgtaaa gtgacattta ttaataatga atctatcatt
     1801 cttcatgttt cataccacag tttatg
//
LOCUS       DAHHSC010000300         1816 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.305, whole genome shotgun sequence.
ACCESSION   DAHHSC010000300 DAHHSC010000000
VERSION     DAHHSC010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1816)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1816)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1816
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.305"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            388..630
                     /locus_tag="NBI57_002603"
     CDS             388..630
                     /locus_tag="NBI57_002603"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HCD1823522.1"
                     /translation="MKGYQQMSPKGTTRIETKTGHQAQFDWKEGINFKTNDNQIVSLN
                     IGVLLLSYSRFVIMKVTMNKSSDVLFNLFTQAFELM"
     gene            795..950
                     /locus_tag="NBI57_002604"
     CDS             795..950
                     /locus_tag="NBI57_002604"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1823523.1"
                     /translation="MNVRNSYKVKHKYLKVNRSNMITYKSNQYSASAEYCGKTVEVQV
                     YDQKQHV"
     gene            1114..1816
                     /locus_tag="NBI57_002605"
                     /pseudo
     CDS             1114..1816
                     /locus_tag="NBI57_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013726225.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
BASE COUNT          709 a          241 c          294 g          572 t
ORIGIN      
        1 ttattgaggc gaacaacaat gaaattatct ttagacataa atatggattt ttaagttatt
       61 actcttacag atttaccaaa attaaaaatt gttaagcaga acttaatcat gaaataaaca
      121 aaagtgaaat agcaagacat atgggggatg atagaagaac agttgataaa taattaaatg
      181 gttttgagcc gaccaagaag aggaaccgtc agtctattat tgacaagtat tatccaatca
      241 tccaaaagtt actttcacac tagtgaataa tagttctact ataatggtgt attttgacaa
      301 tatttgaagg ttaaacttag attaacctgt gtctattctg catttagagc ttatgtcctt
      361 aagcatgttg tatttaatcg ttattttatg aaaggttatc aacagatgtc gcctaaaggt
      421 acaacgagaa tcgagacaaa aaccggtcat caagcacagt ttgactggaa agaaggcatt
      481 aactttaaaa cgaatgataa tcaaatagtt tcactaaata taggcgtgtt actactttct
      541 tactcccgtt ttgtaatcat gaaagtgaca atgaataaat cgagtgatgt tttatttaat
      601 ttatttacac aagcgtttga gctaatgtga gatgtgcctg atgtacttgt aactgatagt
      661 attatattta gaagaggttc ctaagtttat ttctaatctt aaagagcgac tgaactactc
      721 agttcatgtg agtacaggta aaatgcctat tattgcctta gaaaaagaaa aagttcttac
      781 aatcactacc gaatgtgaat gtaagaaact catataaagt gaaacataaa tatttgaaag
      841 tcaaccgctc taatatgatt acatataagt ccaatcaata ttcagcatca gctgaatatt
      901 gtgggaaaac agttgaagtt caagtttatg atcaaaaaca acacgtttaa tataagacta
      961 aattgatttg tgaacatctc atcacacaac gtaaattaaa ttatcaaaaa gaacattatt
     1021 tagaatcact gccatcctct tttgggaata gagaaaagga tgatattaat cagattacga
     1081 tagataattt aaatgcgatt ggatagtttt atgatgaata attcaacgaa ttataagcgc
     1141 ttaaaagcaa atttagaata tttgaaaatg aatcagatga ttaaccatct tgatgatgtt
     1201 atcgatttta taaacacaac ctgtcattca tagacacatt aattaaatta agcgattaca
     1261 aaatcaaaat taaggagaaa aatttgattg aatcgatggt aaaggttatg gcgttaccgt
     1321 ttaaaaagaa atatcagcct ttgactttaa ttttcaaccg agcatcaatc aacaagatgt
     1381 tttggatttt acggatttac gttttatagg aaaatatgaa aacatagtct ttttagactc
     1441 aaatgtcgtt agtaaaaata gaataagtac ttactttatt aagtgtcatc atttgaaaga
     1501 gaatttaaga aaagcaaaac ttgagaacag cttagaaaat agattgaagc actatagcaa
     1561 gtataagtta ttaatcattg atgagattgg gtacttacct attggaagtg aagctgccaa
     1621 aatattcttc caaattaata gatttaaggt atgagaagaa aagcacaata tttaccacaa
     1681 atatcaattt caacttataa aatgaaatat ttgatgatcc caaaatcgca aatgcgattt
     1741 tggataggat attgcatcat tcaagtgtag taaaaatcac aggaaaatcg tatagattaa
     1801 aaaatcattc acctaa
//
LOCUS       DAHHSC010000301         1810 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.306, whole genome shotgun sequence.
ACCESSION   DAHHSC010000301 DAHHSC010000000
VERSION     DAHHSC010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1810)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1810)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1810
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.306"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            <1..543
                     /locus_tag="NBI57_002606"
     CDS             <1..543
                     /locus_tag="NBI57_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001646493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1672 family protein"
                     /protein_id="HCD1823524.1"
                     /translation="SNDNGDDMSMMVGTVLSGFEYRAQKEKYDNLYKFFKENEKKYQY
                     TGFTKEAINKTQNVGYQNEYFYITYLSRNLKEYRKYYEPLIHKNDKEFKEGMQRARKE
                     LDYTANSNTVATLFSTNDKKNRKEKINNVIDLSEKIERTKDMPIKNTITTQLGNKLIG
                     TKKARFDDKKVVSFGAFEDE"
     gene            536..>1810
                     /locus_tag="NBI57_002607"
     CDS             536..>1810
                     /locus_tag="NBI57_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1823525.1"
                     /translation="MNKINDRDLTELSSYWVYQDINKDNDFTVNGKRFKQVDEYNDNG
                     NKNKKGASDLKIYELLDEKGKPTGEQTMIYQGTSNEAINPNNPLKSLDIGDDWLQNAK
                     LMDNSNKSTDYLKQSDEFADLYRDKLNDANKLSKYNFTQKYGVSPNNYKNKTIVADGG
                     NSEGGAGAKYQGAKHPNEKVVATDPAMVPYAAWQKFARPRFDNMISFNSTNDLLTWLQ
                     DPFIKDMPGKRVNISDGVPRLDALIDSHVGYKRKLNRKDNTYDTVPLIKIKSVKDTEI
                     KNGKKVKKTINITLDMDGRIPINVWTGDSIARSGRGTLIKLNLENLDALSKLITGETS
                     GMLAECVIFLNESFNISENENKNFADRKQQLSEGFKDKINLFQLEEMERTLISKINSL
                     EEVADETIESISAVKHLLPDFALDALKERINEL"
BASE COUNT          769 a          209 c          339 g          493 t
ORIGIN      
        1 agtaatgaca atggtgatga tatgagtatg atggtgggta cagtgctgag tggctttgaa
       61 tatcgagcgc aaaaagaaaa gtatgataac ttatataaat tcttcaaaga aaatgaaaag
      121 aaatatcaat atacaggctt tacaaaagag gcaattaaca agacacaaaa cgttggatat
      181 caaaatgaat acttttatat cacatattta tcaagaaact taaaagaata tcgtaaatat
      241 tacgaaccgt tgattcataa aaatgataaa gaatttaaag agggtatgca acgagctaga
      301 aaagagttag attatactgc taatagtaat acagtagcaa cgttgtttag tacgaatgat
      361 aaaaaaaata gaaaagaaaa gataaataat gtaatagatt tatccgagaa aattgaaaga
      421 acaaaagata tgccaatcaa gaatactata actactcaat taggaaataa acttattggc
      481 acaaaaaaag ctcgttttga tgataagaaa gtagtgtcgt ttggagcatt tgaagatgaa
      541 taaaattaat gatagagact taacagaatt gagtagttac tgggtttatc aagacatcaa
      601 taaagataat gattttacag ttaacggaaa aagatttaag caggttgatg aatataatga
      661 taatggaaat aaaaataaaa aaggtgcctc agatttaaaa atttatgaat tgttggatga
      721 aaaaggaaaa ccaactggtg aacaaaccat gatttatcaa ggaacatcta atgaggcaat
      781 aaaccctaat aatccattaa aatctttaga tatcggagat gattggttac aaaatgcgaa
      841 attaatggat aatagtaata agtcaacgga ttatcttaag caatcagacg aatttgcaga
      901 tttatataga gacaaactaa atgacgctaa taaattaagt aagtataact ttacacaaaa
      961 atatggtgtt agtccaaata attacaaaaa caaaaccatt gtggcggatg gcggtaattc
     1021 ggaaggcggt gcaggagcaa aatatcaagg agcgaaacat ccaaatgaaa aagttgttgc
     1081 tactgaccca gcaatggtac cttatgctgc ttggcagaaa tttgctagac cacgctttga
     1141 taatatgatt agttttaata gtaccaacga tttattaaca tggttacaag atccattcat
     1201 caaagatatg ccaggaaaac gcgttaacat tagtgatggt gtacccaggt tagatgcttt
     1261 aatagacagc catgtaggtt ataaaaggaa gttaaataga aaagacaaca catacgatac
     1321 tgtaccacta atcaaaatta agtcggtaaa agatacagaa attaaaaatg gaaaaaaagt
     1381 aaaaaagact attaacataa cattagatat ggatgggcga attccgataa atgtttggac
     1441 aggggattcg attgcacgtt ctggaagagg aactttaatt aaacttaatt tagaaaatct
     1501 tgatgcgttg agtaaactga ttactggtga aactagtggt atgttagcag aatgcgtaat
     1561 ctttttaaat gaaagtttta acatctcaga aaatgaaaat aaaaattttg cagatagaaa
     1621 gcaacaatta tcagaaggat ttaaggataa gattaactta tttcaattag aagaaatgga
     1681 aagaacttta attagtaaaa taaactcact tgaagaagtt gcagatgaaa caatagaaag
     1741 tattagtgct gttaaacact tattacctga ttttgcattg gatgcattaa aagaaagaat
     1801 taatgagttg
//
LOCUS       DAHHSC010000302         1785 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.308, whole genome shotgun sequence.
ACCESSION   DAHHSC010000302 DAHHSC010000000
VERSION     DAHHSC010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1785)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1785)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1785
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.308"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            57..1625
                     /locus_tag="NBI57_002608"
     CDS             57..1625
                     /locus_tag="NBI57_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine:cation symporter family protein"
                     /protein_id="HCD1823526.1"
                     /translation="MIEKLVTFLNEVVWSKPLVYGLLITGVLFTLRMRFFQVRHFKEM
                     IRLMFQGEKSPNGISSFQAIAMSLAGRVGTGNIVGVSTAIFIGGPGAVFWMWITAFLG
                     ASSAFIESTLGQIFKRVENNEYRGGPAYYIEYGIGGKFGKIYGIIFAIVTIISVGLLL
                     PGVQSNAIASSMHNAIHVPQWLMGAIVVVILGLIIFGGVRSIANVATAVVPFMAIIYI
                     LMAVIIICINIQEVPALFALIFKSAFGLQSAFGGIVGAMIEIGVKRGLYSNEAGQGTG
                     PHAAAAAEVSHPSKQGLVQAFSVYIDTLFVCTATALIILISGTYNVTDGTVNANGTPH
                     LIKDGGIYVENATGKDYSGTAMYAQAGIDKAFHGSGYQFDPTFSGVGSYFIAFALFFF
                     AFTTILSYYYITETNVAYLTRNQNNQVSSIFINIARVIILFATFYGAVKTADVAWAFG
                     DLGVGLMAWLNIIAIWILHKPAVNALKDYEIQKKRLGNGYNAVYQPDPNKLPNAVFWL
                     KTYPERLKQARAKK"
BASE COUNT          526 a          252 c          367 g          640 t
ORIGIN      
        1 agtgtctcgt gcttaatcgt ataatatttt tatgtttaaa caataggggg aatcttatga
       61 ttgaaaaatt agtaaccttt ttaaatgagg ttgtttggag taagccatta gtttatggtt
      121 tgctaattac tggtgtgcta tttacattgc gtatgcgatt ttttcaagtt agacatttta
      181 aagaaatgat tcgattaatg tttcaaggag agaagtctcc taatggtatt tcaagttttc
      241 aagcgatagc catgtcttta gcaggcaggg ttggtacagg taatattgtc ggtgtatcta
      301 ctgcaatatt tataggagga cctggtgcag tattttggat gtggattact gcgtttttag
      361 gtgcaagtag tgcttttatt gaatctacac ttggtcaaat attcaagaga gttgaaaata
      421 atgaataccg tggtggacca gcgtattata ttgaatatgg tattggtggt aaatttggta
      481 aaatttacgg aattatcttt gctattgtta cgattatctc agtaggtcta ttgcttcctg
      541 gtgtgcaatc taacgctata gcaagttcta tgcataatgc gattcatgtt ccacaatggt
      601 taatgggtgc tattgttgta gttattttgg gattaattat ttttggtggt gtacgtagta
      661 ttgccaatgt tgcaacagcc gttgtaccat ttatggcaat tatttacata ctgatggctg
      721 tcattatcat ttgtatcaat atacaagaag tgccagcgtt atttgcatta attttcaaat
      781 cagcatttgg attacaatct gcttttggtg gtatcgttgg cgcaatgata gagattggtg
      841 ttaaacgtgg attatactca aatgaggctg gtcaaggtac aggtccacac gcagcagcgg
      901 cagcagaagt atcacatcca agtaaacaag gtctagtaca agcattttca gtttatattg
      961 atacattatt tgtatgtact gcaactgctc tgattatact tatttctggt acatataatg
     1021 tgactgatgg tacggttaat gcgaatggca caccgcattt aattaaagat ggcggtattt
     1081 atgttgaaaa tgcaacaggt aaagattatt caggtactgc gatgtatgca caagccggca
     1141 ttgataaagc gttccatggc agtggttatc aatttgatcc tactttctct ggcgtaggtt
     1201 cgtactttat tgcatttgct ttattcttct ttgcatttac tacaattttg tcgtactact
     1261 acattacaga aacaaatgtt gcttatttaa cgcgtaatca aaataatcaa gtttcatcga
     1321 tatttattaa tattgctcgt gtgattattt tgttcgctac attttacggt gcagttaaaa
     1381 cagctgatgt agcatgggca ttcggtgatt taggtgtagg tctaatggct tggttaaata
     1441 tcattgcgat ttggatttta cataagcctg ccgtaaatgc tttaaaagat tatgaaattc
     1501 aaaagaaacg tttaggaaac ggttataatg cagtttatca acctgatccg aataaattac
     1561 ctaatgctgt cttttggttg aagacatatc cagaacgttt aaaacaagca cgtgccaaaa
     1621 agtaatctac ttttgtttat agtatatgta gtgatcattt gataaaaaaa gaaacagcat
     1681 tgataatttt aggtgctcag aaatttgaat tttaaaaata tagtgtctct tggtacaata
     1741 acaatacaac tactaggggg cactttttta tgtcagaatt taaaa
//
LOCUS       DAHHSC010000303         1768 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.309, whole genome shotgun sequence.
ACCESSION   DAHHSC010000303 DAHHSC010000000
VERSION     DAHHSC010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1768)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1768)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1768
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.309"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            89..1384
                     /gene="purB"
                     /locus_tag="NBI57_002609"
     CDS             89..1384
                     /gene="purB"
                     /locus_tag="NBI57_002609"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HCD1823527.1"
                     /translation="MIERYSREEMSNIWTDQNRYEAWLEVEILACEAWSELGHIPKAD
                     VQKIRQNAKVNVERAQEIEQETRHDVVAFTRQVSETLGEERKWVHYGLTSTDVVDTAL
                     SFVIKQANDIIEKDLERFIDVLAEKAKNYKYTLMMGRTHGVHAEPTTFGVKMALWYTE
                     MQRNLQRFKQVREEIEVGKMSGAVGTFANIPPEIESYVCKHLGIGTAPVSTQTLQRDR
                     HAYYIATLALIATSLEKFAVEIRNLQKTETREVEEAFAKGQKGSSAMPHKRNPIGSEN
                     ITGISRVIRGYITTAYENVPLWHERDISHSSAERIMLPDVTIALDYALNRFTNIVDRL
                     TVFEDNMRNNIDKTFGLIFSQRVLLALINKGMVREEAYDKVQPKAMISWETKTPFREL
                     IEQDESITSVLTKEELDECFDPKHHLNQVDTIFERAGLA"
     gene            1493..>1768
                     /locus_tag="NBI57_002610"
     CDS             1493..>1768
                     /locus_tag="NBI57_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1823528.1"
                     /translation="MQIEKLRGAALDELFDAILTLENREECYQFFDDLCTVNEIQSLS
                     QRLQVAKMIKQGYTYATIEQESGASTATISRVKRSLQWGNDAYTMILD"
BASE COUNT          655 a          252 c          342 g          519 t
ORIGIN      
        1 tagagttaaa aacgaacttt ttgtttattt ttattaaaaa tgttctgttt ttgaaaattc
       61 gagtatacat acgataggag ttaaaaaaat gattgaacgc tattctagag aagaaatgtc
      121 taatatttgg actgatcaaa atcgctatga agcatggtta gaagtggaaa ttttagcatg
      181 tgaagcatgg agtgaattag gacatatacc gaaagctgac gtacaaaaaa ttcgtcaaaa
      241 cgcaaaggta aatgtcgaac gtgcacaaga aattgaacaa gaaacgcgcc atgatgttgt
      301 agcctttact agacaagttt ctgaaacgct aggtgaagaa cgtaagtggg tacattatgg
      361 tttaacttct actgatgttg tagatacagc tttaagtttc gttattaaac aagcaaatga
      421 cattattgaa aaagatttag aaagatttat cgacgtttta gctgaaaaag caaaaaatta
      481 taaatataca ttgatgatgg gacgtacaca tggagtgcat gcagaaccaa ctacatttgg
      541 tgtcaaaatg gcattgtggt acacggaaat gcaacgtaac ttacaacgtt tcaaacaagt
      601 aagagaagaa attgaagtag ggaaaatgag tggtgcagta ggtacttttg ctaacattcc
      661 tcctgaaata gaaagttatg tgtgtaaaca tttaggtatt ggcacagcgc ctgtatcaac
      721 gcaaacattg caacgcgatc gacatgcata ctatattgcg acattagcat taatcgcgac
      781 gtcactagaa aaatttgccg ttgaaattcg taaccttcaa aaaaccgaaa caagagaagt
      841 tgaagaagca tttgcaaaag gccaaaaagg ttcatctgca atgcctcata aacgaaatcc
      901 aattggttct gaaaatatca caggtatttc aagagtcatt cgtggttata ttactacagc
      961 ttatgagaat gtgccattat ggcatgaaag agatatttct cattcttctg ctgagcgtat
     1021 tatgttacca gatgttacaa tagcactaga ttatgcattg aatcgtttca caaatattgt
     1081 ggaccgttta actgtatttg aagataatat gcgtaataac atagataaaa catttggtct
     1141 gattttctca caacgtgtct tattagcatt aattaataaa ggtatggttc gtgaagaagc
     1201 atacgacaaa gtacaaccaa aagcgatgat atcttgggaa acgaaaacac ctttccgtga
     1261 attaatcgaa caagatgagt ctattacaag tgttttaaca aaagaagaat tagatgaatg
     1321 tttcgatcct aaacatcatt tgaatcaagt tgacactatt ttcgaacgcg ctggattagc
     1381 ataaaattaa ataaagtaac gattcagatt taataaaata taaatcgcta cactttaata
     1441 gattaatgcg ttaaaatgac ataaagaata aaattatagg ggttgtttcg atatgcaaat
     1501 tgaaaaatta cgaggtgcag cgttagatga attgtttgat gcaatactaa cgttagaaaa
     1561 tagagaagaa tgttaccaat ttttcgatga tttgtgtact gtaaatgaaa ttcaatcact
     1621 gtctcaaaga ttacaagttg ctaaaatgat taagcaaggt tatacctatg caacgattga
     1681 acaagaatct ggagcatcga ctgcaacgat ttctagagtg aaacgttcat tacaatgggg
     1741 taatgatgct tatacaatga ttttagat
//
LOCUS       DAHHSC010000304         1760 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.310, whole genome shotgun sequence.
ACCESSION   DAHHSC010000304 DAHHSC010000000
VERSION     DAHHSC010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1760)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1760)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1760
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.310"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            <1..1373
                     /locus_tag="NBI57_002611"
     CDS             <1..1373
                     /locus_tag="NBI57_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="class D beta-lactamase"
                     /protein_id="HCD1823529.1"
                     /translation="YLKKQSLYLNENEKNKIDTILFNHQYKKNIVIRKAETIQSPITF
                     WYGKYIILIPSSYFKSVIDKRLKYIILHEYAHAKNRDTLHLIIFNIFSIVMSYNPLIH
                     IVKRKIIHDNEVEADRFVLNNINKNEFKTYAESIMDSVLNIPFFNKNILSHSFNGKKS
                     LLKRRLINIKEANLKKQSKLIPIFICIFTFLLMVIQSQFLMGQSITDYNYKKPLQNDH
                     QILDESKNFGSNSGSFVMYSMKKDKYYIYNEKESRKRYSPDSTYKIYLAMFGLDHHII
                     SDKNSRMSWNHKHYPFESWNKEQDLNTAMQNSVNWYFERISNQIPKNYTAAQLKQLNY
                     GNENLGSYKSYWMEDSLKISNLEQVIVFKNMMEQNNHFSKKAKNQLSSSLLIKKNEKY
                     ELYGKTGTGIVNGKYNNGWFVGYVITNHDKYYFATHLSDGKPSGKNAELISEKILKEM
                     GVLNGQ"
     gene            1363..1743
                     /gene="blaI"
                     /locus_tag="NBI57_002612"
     CDS             1363..1743
                     /gene="blaI"
                     /locus_tag="NBI57_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillinase repressor BlaI"
                     /protein_id="HCD1823530.1"
                     /translation="MANKQVEISMAEWDVMNIIWDKKSVSANEIVVEIQKYKEVSDKT
                     IRTLITRLYKKEIIKRYKSENIYFYSSNIKEDDIKMKTAKTFLNKLYGGDMKSLVLNF
                     AKNEELNNKEIEELRDILNDISKK"
BASE COUNT          746 a          194 c          265 g          555 t
ORIGIN      
        1 aatatttaaa gaaacagtca ctttatctaa acgaaaatga aaaaaataaa atagatacga
       61 tacttttcaa ccatcaatat aaaaaaaata ttgtgattcg aaaagcagag actattcaat
      121 ctccaataac tttttggtat gggaaatata ttattttgat tcctagttca tattttaaaa
      181 gtgtaattga taaaagacta aaatatataa tactgcatga atatgctcat gctaaaaata
      241 gagatacttt acatttaatt atctttaata tcttctctat tgttatgagt tacaatccat
      301 taatacatat tgtaaaaaga aagataattc atgacaatga agtagaagcc gatagatttg
      361 ttcttaataa tattaacaaa aatgaattta aaacttatgc ggaatctatt atggactccg
      421 tattaaatat tccatttttt aataaaaata tattaagcca ttcatttaat ggtaaaaagt
      481 cattactcaa aagaagatta attaatataa aagaagccaa tttgaaaaaa cagtccaaat
      541 taattccaat ttttatttgt atttttactt ttttgctcat ggtaattcaa agccagtttt
      601 tgatggggca atccataact gattataatt ataaaaagcc cttacagaac gatcaccaaa
      661 ttttagatga aagtaaaaat tttggttcaa atagtggatc tttcgtcatg tacagcatga
      721 agaaagacaa atactatatt tataatgaaa aagaaagtag aaaaagatat tctcctgatt
      781 caacttataa aatttattta gcgatgtttg gacttgacca tcatatcata agtgataaaa
      841 attcacgtat gtcatggaat cataaacatt atcctttcga gtcttggaat aaggaacaag
      901 atttaaatac agcaatgcaa aattcagtta attggtactt cgaacgtatt agtaatcaaa
      961 taccaaagaa ctatactgcg gctcaactca agcaattaaa ttatggtaat gaaaacttgg
     1021 gaagttataa aagctattgg atggaagata gtttgaaaat atctaatctt gaacaagtaa
     1081 tagtttttaa aaatatgatg gaacaaaata accattttag taaaaaagca aagaatcaat
     1141 tatcttcttc attattgatt aagaaaaatg aaaagtatga actgtatggg aaaacaggta
     1201 caggtatagt aaacgggaag tataataatg ggtggtttgt aggttacgta attacaaatc
     1261 atgataagta ttattttgct acacatttat cagatggaaa gccgtctggg aaaaatgctg
     1321 aattaatcag tgaaaaaata ttaaaagaaa tgggtgtttt aaatggccaa taagcaagtt
     1381 gaaatatcta tggctgaatg ggatgttatg aatataatat gggataaaaa atcagtatca
     1441 gctaatgaaa ttgtagttga aattcaaaaa tataaagaag ttagcgataa aacgattaga
     1501 acattaatca caagactata taaaaaagag attataaaac gatacaaatc agagaatatt
     1561 tatttttact catcaaatat taaagaagac gatattaaaa tgaaaactgc taaaaccttt
     1621 cttaataaac tgtatggagg ggacatgaaa agtttagtgc tgaattttgc gaaaaatgaa
     1681 gaattaaata acaaagaaat tgaagaattg cgagacattt taaatgatat tagtaaaaag
     1741 taattagaga tatgtttcaa
//
LOCUS       DAHHSC010000305         1741 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.311, whole genome shotgun sequence.
ACCESSION   DAHHSC010000305 DAHHSC010000000
VERSION     DAHHSC010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1741)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1741)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1741
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.311"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            <1..552
                     /locus_tag="NBI57_002613"
     CDS             <1..552
                     /locus_tag="NBI57_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCD1823531.1"
                     /translation="ITLCGFVICYGLIFGLFYLLSGSRADYLIVTIVISAIISIFVII
                     LSIVPVIVLASDLFKERISKGVILIVLAIIALVLCNFVSAILWFVSAISILGRKKLVA
                     AADTTTIQKSKGNANQASHKDTCKKELDSQDMMEHPEVKNPTTKNLEGFNEEIHKDEA
                     TTKVVSDNTEPPIESKGHVSKKD"
     gene            764..1540
                     /locus_tag="NBI57_002614"
     CDS             764..1540
                     /locus_tag="NBI57_002614"
                     /EC_number="1.1.1.304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(S)-acetoin forming diacetyl reductase"
                     /protein_id="HCD1823532.1"
                     /translation="MTNNKVALVTGGAQGIGFKIAERLVEDGFKVAVVDFNEEGAKAA
                     ALKLSSDGTKAIAIKADVSNRDDVFNAVRQTAAQFGDFHVMVNNAGLGPTTPIDTITE
                     EQFKTVYGVNVAGVLWGIQAAHEQFKKFNHGGKIINATSQAGVEGNPGLSLYCSTKFA
                     VRGLTQVAAQDLASEGITVNAFAPGIVQTPMMESIAVATAEEAGKPEAWGWEQFTSQI
                     ALGRVSQPEDVSNVVSFLAGKDSDYITGQTIIVDGGMRFR"
BASE COUNT          615 a          272 c          347 g          507 t
ORIGIN      
        1 ataactttat gtgggtttgt aatttgctat ggactaattt tcggcctttt ctatttatta
       61 tcaggtagca gagctgatta tttaatagta acaatagtta tatcggcaat aatttctata
      121 tttgtaatta tactttcaat cgtacctgtc atcgtattgg catctgactt atttaaagaa
      181 aggatttcaa aaggtgtcat attaattgta ttggctatta ttgctttagt attatgcaac
      241 tttgtatctg caatactctg gtttgtttca gccatatcta ttttaggtag aaaaaaatta
      301 gtagctgcag cagatactac cactattcaa aaaagtaaag ggaacgcaaa tcaagcatca
      361 cataaagaca cgtgtaaaaa ggaacttgat agtcaagaca tgatggaaca tcctgaggtt
      421 aaaaatccca cgactaaaaa ccttgaagga tttaacgaag aaatacataa agatgaagct
      481 acaactaaag ttgtcagtga taacacggaa ccgccgattg aatcaaaagg ccatgtctcg
      541 aaaaaagatt gatgacaaac taatcgagag acttaaaaaa ataatattca acataagaac
      601 ttttaaaacg acatttaaac gcattgccaa tcactaatgg tagtgcgttt aactatacct
      661 taaatatctg aatattttgt taaatggagc tacctttgtt gtactattca aatgaagagg
      721 agtaaaatgt aattaaagga aagaaatttg aggagtgatc tttatgacaa acaacaaagt
      781 agcattagta actggcggag cacaagggat tggttttaaa attgcagaac gtttagtgga
      841 agatggtttc aaagtagcag ttgttgattt caatgaagaa ggggcaaaag cagctgcact
      901 taaattatca agtgatggta caaaagctat tgctatcaaa gcagatgtat caaaccgtga
      961 tgatgtattt aacgcagtaa gacaaactgc cgcgcaattt ggcgatttcc atgtcatggt
     1021 taacaatgcc ggccttggac caacaacacc aatcgataca attactgaag aacagtttaa
     1081 aacagtatat ggcgtgaacg ttgcaggtgt gctatggggt attcaagccg cacatgaaca
     1141 atttaaaaaa ttcaatcatg gcggtaaaat tatcaatgca acatctcaag caggcgttga
     1201 gggtaaccca ggcttgtctt tatattgcag tacaaaattc gcagtgcgag gtttaacaca
     1261 agtagccgca caagatttag cgtctgaagg tattactgtg aatgcattcg cacctggtat
     1321 cgttcaaaca ccaatgatgg aaagtatcgc agtggcaaca gccgaagaag caggtaaacc
     1381 tgaagcatgg ggttgggaac aatttacaag tcagattgct ttgggcagag tttctcaacc
     1441 agaagatgtt tcaaatgtag tgagcttctt agctggtaaa gactctgatt acattactgg
     1501 acaaacaatt attgtagatg gtggtatgag attccgttaa taatcatgca ttagcgataa
     1561 ataaatcctt attgttaagt ttaatcactt agcagtaagg attttttagt gcacttagaa
     1621 gggattgtat tggtagaaaa ttaataagcg aagttcttaa atgagttatg atgtcacagt
     1681 ctaatgcatc agttgaaagc attactagta ttaacacacc caagatatta taaaacatca
     1741 c
//
LOCUS       DAHHSC010000306         1737 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.312, whole genome shotgun sequence.
ACCESSION   DAHHSC010000306 DAHHSC010000000
VERSION     DAHHSC010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1737)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1737)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1737
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.312"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            186..1646
                     /locus_tag="NBI57_002615"
     CDS             186..1646
                     /locus_tag="NBI57_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine:cation symporter family protein"
                     /protein_id="HCD1823533.1"
                     /translation="MKDFDSLIPGWFKEFVHVGTDLIWSQYLIGLLLTAGFFFTISSK
                     FVQLRMLPEMFRALVERPETLEDGKKGISPFQAFAISAGSRVGTGNIAGVATAIVLGG
                     PGAVFWMWVIAFIGAASAFIEATLAQVYKVHDKDGGFRGGPAYYITKGLNQKWLGIVF
                     AILITITFAFVFNTVQSNTIAESLNTQYNISPVITGIILAIVTAIIIFGGVRSIATLS
                     SLIVPIMAIIYIGMVLVILLFNLDQIVPMIGTIIKSAFGIEQVTGGAVGAAVLQGIKR
                     GLFSNEAGMGSAPNAAATAAVPHPVKQGLIQSLGVFFDTMLVCTATAIMILLYSGLKF
                     GDNAPQGVAVTQSALNEHLGSAGGIFLTIAVTLFAFSSVVGNYYYGQSNIEFLSTNRV
                     ILFIFRCLVVVLVFVGAVVKTETVWNTADLFMGLMAIVNIISIIGLSNVAFALMKDYQ
                     KQKKEGKNPVFKPENLEINLFGISAWGANKYKNSDK"
BASE COUNT          518 a          243 c          354 g          622 t
ORIGIN      
        1 agggatttat attaaatgct gaccaagtac ttatcgttaa attagcgata gactttgacc
       61 aaactaatat aaatctcttg tattgtataa gaaatgatga tgtatctcta tatttactga
      121 agtttcaatt cggtctaatt ttatagaagg tgcatgattc atgtagagga tgcgaggggt
      181 ttattttgaa agatttcgat agtttaattc ctggatggtt taaagaattt gtccatgttg
      241 gtaccgattt aatatggtct caatatttaa ttggtctatt attgacagct ggattcttct
      301 ttacaattag ttctaaattc gttcaattac gaatgttacc tgaaatgttt agagctttag
      361 tagaacgtcc agaaacttta gaagatggta agaagggtat ttcgccattc caagcatttg
      421 cgattagtgc tggttcgaga gttggtactg gtaatattgc tggtgttgcg actgcgattg
      481 ttttaggcgg tccaggtgca gtgttttgga tgtgggttat tgcatttata ggtgcagcga
      541 gtgcatttat agaagcgact ttggctcagg tttataaagt acatgataaa gatggtggat
      601 tccgtggtgg tccagcttac tatattacta aaggtttaaa tcaaaaatgg ctaggtatcg
      661 tatttgcgat tttaattaca attacatttg catttgtatt taacacagtg caatctaata
      721 caattgcgga gtcgttaaat acgcaatata atattagtcc agtaatcaca ggtattattt
      781 tagcaatcgt aacagctatt attatatttg gtggtgtacg tagtattgct acgttatctt
      841 cgttaattgt accgattatg gctatcattt acattggtat ggttttagta atattgctat
      901 ttaatttaga tcaaattgtt cctatgatag gtacgattat taaaagtgca tttggtatcg
      961 aacaagtaac tggtggcgct gtaggtgctg cggttcttca aggtatcaaa cgtggtttat
     1021 tctctaacga agctggtatg ggttctgcgc cgaatgcagc ggcaactgct gccgtaccac
     1081 accctgttaa gcaaggttta atccaatcat taggtgtgtt ctttgataca atgttggttt
     1141 gtacagcaac tgcaatcatg attttactat attcaggatt gaaatttggt gataacgcac
     1201 ctcaaggtgt tgcagttact caatcagcac ttaatgagca tttaggttct gctggaggta
     1261 ttttcttaac aatagcagtt acactgtttg cattttcatc tgttgtaggt aattactatt
     1321 acggtcaatc taatattgaa tttttatcaa caaatcgtgt gatattattt atctttagat
     1381 gtcttgttgt agtacttgtc tttgtcggtg cagttgtaaa aacagaaaca gtatggaata
     1441 cggcagactt atttatgggc ttaatggcaa ttgtaaacat tatttccatt ataggactgt
     1501 ccaatgtagc ttttgcattg atgaaagatt atcaaaagca gaaaaaagaa ggcaagaacc
     1561 ctgtctttaa acctgaaaac ttagaaatta acttatttgg aattagtgct tggggcgcta
     1621 acaaatataa gaactctgat aaataaatat ttttaatgta acgattcatt atataaaaat
     1681 caaataaacc ctgaggtttt caaattaaag cgaaaatctc agggttttaa atttgta
//
LOCUS       DAHHSC010000307         1710 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.313, whole genome shotgun sequence.
ACCESSION   DAHHSC010000307 DAHHSC010000000
VERSION     DAHHSC010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1710)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1710)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1710
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.313"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            55..381
                     /locus_tag="NBI57_002616"
     CDS             55..381
                     /locus_tag="NBI57_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019468012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor antagonist"
                     /protein_id="HCD1823534.1"
                     /translation="MNLNIETTTQDKFYEVKVGGELDVYTVPELEEVLTPMRQDGTRD
                     IYVNLENVSYMDSTGLGLFVGTLKALNQNDKELYILGVSDRIGRLFEITGLKDLMHVN
                     EGTEVE"
     gene            383..862
                     /gene="rsbW"
                     /locus_tag="NBI57_002617"
     CDS             383..862
                     /gene="rsbW"
                     /locus_tag="NBI57_002617"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001190828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma B factor RsbW"
                     /protein_id="HCD1823535.1"
                     /translation="MQSKEDFIEMRVPASAEYVSLIRLTLSGVFSRAGATYDDIEDAK
                     IAVSEAVTNAVKHAYKENNNVGIINIYFEILEDKIKIVISDKGDSFDYETTKSKIGPY
                     DKDENIDFLREGGLGLFLIESLMDEVTVYKESGVTISMTKYIKKEQVRNNGERVEIS"
     gene            837..1607
                     /gene="sigB"
                     /locus_tag="NBI57_002618"
     CDS             837..1607
                     /gene="sigB"
                     /locus_tag="NBI57_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor SigB"
                     /protein_id="HCD1823536.1"
                     /translation="MAKESKSANEVSPEQINQWIKEHQENKNTDAQDKLVKHYQKLIE
                     SLAYKYSKGQSHHEDLVQVGMVGLIGAINRFDMSFERKFEAFLVPTVIGEIKRYLRDK
                     TWSVHVPRRIKEIGPRIKKVSDELTAELERSPSISEIANRLEVSEEEVLEAMEMGQSY
                     NALSVDHSIEADKDGSTVTLLDIMGQQDDHYDLTEKRMILEKILPILSDREREIIQCT
                     FIEGLSQKETGERIGLSQMHVSRLQRTAIKKLQEAAHK"
BASE COUNT          647 a          213 c          342 g          508 t
ORIGIN      
        1 gatacagcgc ttaaaatgaa gataaatatt tttaataagt aggagtgtaa tgaaatgaat
       61 cttaatatag aaacaaccac tcaagataaa ttttacgaag ttaaagtcgg tggagaatta
      121 gatgtttata ctgtgcctga attagaagag gttttaacac ctatgagaca agatggaact
      181 cgtgatattt atgttaattt agaaaatgtg agttatatgg attcgacagg tttaggttta
      241 ttcgtaggta cattaaaagc attaaaccaa aatgataaag aactatacat tttaggtgtg
      301 tcagatcgta tcggtagact atttgaaatt actggtctta aggatttaat gcatgttaat
      361 gaaggaacgg aggtcgaata acatgcaatc taaagaagat tttatcgaaa tgcgcgtgcc
      421 agcatcggca gagtatgtaa gtttaattcg tttaacactt tctggcgttt tttcgagagc
      481 tggtgctaca tatgatgata ttgaagatgc caagattgca gttagtgaag ctgtgacaaa
      541 tgcagttaaa catgcataca aagaaaataa caatgtgggc attattaaca tatattttga
      601 aattttagaa gataaaatta aaattgttat ttctgataaa ggtgacagtt ttgattatga
      661 aacaactaaa tcaaaaatag gtccttacga taaagacgaa aatatagact ttttacgcga
      721 aggtggccta ggtttatttt taatcgaatc tttaatggat gaagtcacag tatataaaga
      781 atctggtgtg acaatcagta tgactaagta tataaaaaaa gagcaggtgc gaaataatgg
      841 cgaaagagtc gaaatcagct aatgaagttt cacctgagca aattaaccaa tggattaaag
      901 aacaccaaga aaataagaat acagatgcac aggataagtt agttaaacat taccaaaaac
      961 taattgagtc attggcatat aaatattcta aaggacaatc acatcacgaa gatttagttc
     1021 aagttggtat ggttggttta ataggtgcca taaatagatt cgatatgtcc tttgaacgga
     1081 agtttgaagc ctttttagta cctactgtaa tcggtgaaat caaaagatat ctacgagata
     1141 aaacttggag tgtacatgtt ccgagacgta ttaaagaaat tgggccaaga atcaaaaaag
     1201 tgagcgatga actaaccgct gaattagagc gttcaccttc tatcagtgaa atagctaatc
     1261 gattagaagt ctcagaagaa gaagtgttag aagcaatgga aatgggacaa agttataatg
     1321 cgttaagtgt tgatcattcc attgaagctg ataaagatgg ttcaactgtt acgctattag
     1381 atattatggg gcaacaagat gaccattatg acttaactga aaaacgtatg attttagaaa
     1441 aaatattacc tatattatct gatcgcgaac gagaaatcat acaatgtacg tttattgaag
     1501 gattgagtca aaaagagaca ggtgagcgta tcggtttaag tcaaatgcat gtatcacgac
     1561 ttcagagaac ggcaattaag aaattacaag aagcagcaca taaatagaat ttgcttatta
     1621 atgatacgtt ttataatgaa aaatccatat aattatccct tgattattaa attgaaatcg
     1681 aggggtattt ttaatttaat taagattttc
//
LOCUS       DAHHSC010000308         1684 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.314, whole genome shotgun sequence.
ACCESSION   DAHHSC010000308 DAHHSC010000000
VERSION     DAHHSC010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1684)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1684)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1684
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.314"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            complement(89..1570)
                     /gene="cls"
                     /locus_tag="NBI57_002619"
     CDS             complement(89..1570)
                     /gene="cls"
                     /locus_tag="NBI57_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HCD1823537.1"
                     /translation="MRYTFSNDLGTLFTIILAIGFIINLVLAFIIIFLERNRRTASST
                     WAWLFVLFVLPLIGFILYLFFGRTVSARKLNKNNGNVLTDFDGLLKQQIESFDKGNYG
                     TDNKQVQKHHDLVRMLLMDQDGFLTENNKVDHFIDGNDLYDQVLKDIKNAKEYIHLEY
                     YTFALDGLGKRILHALEEKLKQGLEVKILYDDVGSKNVKMANFDHFKSLGGEVEAFFA
                     SKLPLLNFRMNNRNHRKIIVIDGQLGYVGGFNIGDEYLGLGKLGYWRDTHLRIQGDAV
                     DALQLRFILDWNSQAHRPQFEYDVKYFPKKNGPLGNSPIQIAASGPASDWHQIEYGYT
                     KMIMSAKKSVYLQSPYFIPDNSYINAIKIAAKSGVDVHLMIPCKPDHPLVYWATFSNA
                     SDLLSSGVKIYTYENGFIHSKMCLIDDEIVSVGTANMDFRSFELNFEVNAFVYDENLA
                     KDLRVAYEHDITKSKQLTKESYANRPLSVKFKESLAKLVSPIL"
BASE COUNT          576 a          301 c          220 g          587 t
ORIGIN      
        1 ttattaggtt tacataatat gtttatcgtt attttaattt tgggcaaaat aaaaagagcc
       61 tctataatcg agactcctta caaataaatt ataaaattgg cgaaactaat tttgctaacg
      121 attctttgaa tttaacagac agcggtctat tggcatatga ttctttggtt agttgttttg
      181 attttgtaat atcatgttca taagccaccc ttaaatcctt agcaagattt tcatcatata
      241 caaaggcatt tacttcaaaa tttaattcaa aacttctaaa gtccatattt gctgtgccca
      301 ctgatacgat ttcatcatca attaagcaca ttttagaatg tataaatcca ttttcatacg
      361 tataaatttt aacaccactt gataataagt cagaggcatt tgaaaatgtc gcccaataaa
      421 ctaaaggatg atctggctta catggaatca ttagatgaac atctacacct gatttagctg
      481 caattttaat ggcatttata tatgagttat ccggaatgaa ataaggcgat tgcaaatata
      541 ccgacttctt agcgctcata atcatttttg tataaccata ttcaatttga tgccagtcac
      601 tagctgggcc acttgcagct atttgaattg gtgaattgcc caatggtccg ttctttttag
      661 ggaaatactt aacatcatat tcaaactgtg gacggtgcgc ttgcgaattc cagtctaaaa
      721 taaatcgcaa ctgcagtgca tcaaccgcat ccccttgtat acgtaaatgc gtatctctcc
      781 aataccctag tttacctaat cctagatatt catcaccaat gttaaatcct ccgacataac
      841 ctagttgacc atcgattacg atgatttttc tatgatttct gttattcata cggaaattta
      901 ataagggtaa tttagaagca aaaaatgctt caacttcgcc acctaatgat ttaaagtgat
      961 caaaatttgc catcttaaca tttttagatc caacatcatc atataatatt tttacttcta
     1021 gaccttgttt caatttttct tctaaagcat gtaaaattct tttacctaaa ccatctaaag
     1081 cgaaagtata gtactctaaa tggatatatt cttttgcatt tttaatatct tttaaaactt
     1141 gatcatataa atcatttcca tcaatgaaat gatcaacttt attattttca gttaaaaaac
     1201 catcttgatc catcaaaagc atacgtacta aatcatgatg tttttgaact tgtttgttat
     1261 cagtaccata attaccttta tcaaagcttt ctatttgttg ttttaaaagt ccatcgaaat
     1321 ccgttaacac gttaccattg tttttattca atttacgtgc cgaaacggtt ctaccaaaaa
     1381 acaagtaaag aataaaacca atcaatggta agacaaaaag tacaaatagc catgcccaag
     1441 ttgaactcgc tgtacgtcta tttctttcta aaaagataat aataaaagcc aatactaaat
     1501 taatgatgaa tccaatggct aaaataatgg taaaaagtgt tcctaaatca tttgaaaatg
     1561 tatatcgcat agtcttttct cctataaaga aaggcacaaa aaacattttg cacctttcac
     1621 atcatattat ttattcacag ataaagttaa aattgcattg aattcttctt cattatttgg
     1681 gaat
//
LOCUS       DAHHSC010000309         1650 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.316, whole genome shotgun sequence.
ACCESSION   DAHHSC010000309 DAHHSC010000000
VERSION     DAHHSC010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1650)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1650)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1650
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.316"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            44..1186
                     /gene="scpA"
                     /locus_tag="NBI57_002620"
     CDS             44..1186
                     /gene="scpA"
                     /locus_tag="NBI57_002620"
                     /EC_number="3.4.22.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine protease staphopain A"
                     /protein_id="HCD1823538.1"
                     /translation="MKRNFPKLIALSLIFSLSVTPIANAESNSNIKAKDKKHVQVNVE
                     DKSVPTDVRNLAQKDYLSYVTSLDKIYNKEKASYTLGEPFKIYKFNKKSDGNYYFPVL
                     NTEGNIDYIVTISPKITKYSSSSSKYTINVSPFLSKVLNQYKDQQITILTNSKGYYVV
                     TQNHKAKLVLKTPRLEDKKLKKTESIPTGNNVTQLKQKASVTMPTSQFKSNNYTYNEQ
                     YINKLENFKIRETQGNNGWCAGYTMSALLNATYNTNKYHAEAVMRFLHPNLQGQRFQF
                     TGLTPREMIYFGQTQGRSPQLLNRMTTYNEVDNLTKNNKGIAVLGSRVESRNGMHAGH
                     AMAVVGNAKLDNGQEVIIIWNPWDNGFMTQDAKNNVIPVSNGDHYR"
     gene            1241..1567
                     /locus_tag="NBI57_002621"
     CDS             1241..1567
                     /locus_tag="NBI57_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphostatin A"
                     /protein_id="HCD1823539.1"
                     /translation="MEQIELFSIDKFKCNSEAKYYLNIIEGEWHPQDLNDSPLKFILS
                     TSDDSDYICKYINTEHKQLTLYNKNNSSIVIEIFIPNDNKILLTIMNTEALGTSPRMT
                     FIKHKS"
BASE COUNT          652 a          232 c          247 g          519 t
ORIGIN      
        1 aatttaatat aattatttta taataaaata aaggagtttt tatatgaaaa gaaactttcc
       61 aaaattaata gctttaagtt taatattttc tttaagtgtt acccctattg caaacgctga
      121 gagcaattca aatatcaaag caaaagataa gaagcatgtg caagttaatg tcgaggacaa
      181 gagtgttcct actgacgtac gtaatttagc gcaaaaagac tatctttctt atgttacttc
      241 acttgataaa atctataata aagagaaagc ctcgtataca cttggtgaac cttttaaaat
      301 ttataaattc aacaaaaaga gtgatggtaa ttattatttt ccagttttga acactgaagg
      361 taatatcgat tatatagtta caatttcacc aaaaatcact aagtattcta gttcatcttc
      421 taaatacaca atcaatgttt ctccattttt atctaaagtc ttaaatcaat ataaagatca
      481 acaaattact attttgacaa actcaaaagg ttactatgtg gttactcaaa atcataaagc
      541 taaactagta ctaaaaacac cacgtctaga agataaaaag ttaaaaaaga ctgagagtat
      601 acctactggc aataatgtaa cgcaactaaa gcaaaaagct tctgtcacta tgccaactag
      661 tcaatttaaa agtaataatt atacttacaa cgagcaatat ataaataaac ttgagaattt
      721 caagataaga gaaactcaag gtaacaatgg ttggtgtgca ggctatacga tgtctgcatt
      781 actaaatgcg acttataata ctaacaaata tcatgcagaa gctgtgatga gatttttaca
      841 cccaaactta caaggacaac gattccaatt tactggttta acgccaagag agatgattta
      901 ttttggacaa actcagggaa gaagtccaca acttcttaat agaatgacta catataatga
      961 agttgacaat cttactaaaa ataataaagg aattgctgtt ttaggtagta gagtagaatc
     1021 aagaaatggt atgcacgcag ggcatgccat ggcagttgtt gggaacgcta aattagataa
     1081 tggacaagag gttataatta tatggaatcc atgggataat ggttttatga ctcaagatgc
     1141 taaaaacaat gttattccag tatccaatgg cgaccattat cgatagtatt catctattta
     1201 tggttattaa aatttgtatt ttagaaaggt aaataatatt atggaacaaa ttgaattatt
     1261 tagtattgat aaattcaaat gtaattcaga ggctaagtat tatcttaata ttattgaggg
     1321 agaatggcat cctcaagatt taaatgatag tcctttaaaa tttattctca gtacctcaga
     1381 cgattctgat tacatttgca aatatataaa tacagaacac aaacaactca cattatataa
     1441 taaaaataat agctcaattg ttattgaaat atttatacca aatgataata aaatactact
     1501 aacaattatg aacacagaag ctttgggaac ttctcctaga atgactttca ttaagcataa
     1561 gtcataagac tatctagata tgattaagta aactattaat cttattaaag gtctctagga
     1621 agaaaaaatt tcatgcgatg agattgtttt
//
LOCUS       DAHHSC010000310         1632 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.317, whole genome shotgun sequence.
ACCESSION   DAHHSC010000310 DAHHSC010000000
VERSION     DAHHSC010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1632)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1632)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1632
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.317"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            <1..1117
                     /locus_tag="NBI57_002622"
     CDS             <1..1117
                     /locus_tag="NBI57_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="bacteriocin-associated integral membrane family
                     protein"
                     /protein_id="HCD1823540.1"
                     /translation="KTIRFINTVCKSILLVLIASVMIENTSVIKDLNKIKETEKYWNV
                     LDDYYTIEFAPYHETKQSLIDNMLRSEQLVKASEAENNAILFKPKGDSVDNDNFSPDE
                     GNVILVNNQFWSIYYKQFQPDIPIKNQKNNVEVIIPQKFHAMRNEINQAYHSWFEFVQ
                     NKNNKENKLSIQFINKNDYRIFSFDARDSRHLSFIEAPIIVNVQASDLSNDFYYAMIS
                     QGGYLFKNYDALVKNIENYHLDGEISGITNYKDSVMEMYHENNLKLTVLNFSQIIIAI
                     ILIIIILFDVKYYFEQHRKLLVIKKLYGYSTLRANYQYLLINNIVVVFIGILTNVILH
                     SQYIMMIFATILVVQILLQICSLYYHGRRFNEVIKEF"
     gene            1120..1440
                     /locus_tag="NBI57_002623"
     CDS             1120..1440
                     /locus_tag="NBI57_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HCD1823541.1"
                     /translation="MKFIIAILLGLIVSITIAFTIIHHPILDRFNPFLKTEYSYAKVP
                     KGTQQYVNITAYSERGEKLDYKLTFNGFSPSRTYVEIKHKGQYVISITYVEKEDIPKE
                     VRQE"
     gene            1437..>1632
                     /locus_tag="NBI57_002624"
     CDS             1437..>1632
                     /locus_tag="NBI57_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCD1823542.1"
                     /translation="MIELKDLTIQKGNIHILKKLNLKFQCGKSYALIGKSGCGKSTLL
                     NTIAGLEKTGKQYVYFNGQLE"
BASE COUNT          626 a          206 c          263 g          537 t
ORIGIN      
        1 taaaacaatt cgttttatta atacagtttg taaaagtatt ctcttagtac taatagctag
       61 tgtaatgata gaaaatacta gtgttattaa agatttgaat aaaataaagg aaactgagaa
      121 atattggaat gtattagatg attattacac gattgaattt gcaccttatc acgaaacgaa
      181 acaaagtttg attgataata tgctgcgatc agaacaatta gtaaaggcta gtgaagcaga
      241 aaataatgcg attttattca aaccaaaggg tgactccgtt gacaatgaca acttttcgcc
      301 tgatgagggg aatgtaatat tagtaaataa tcagttctgg tcgatttatt acaagcagtt
      361 tcaacctgat attccgataa aaaatcaaaa aaataatgtc gaagtaatta ttccacaaaa
      421 gtttcatgca atgcgtaatg aaatcaatca agcatatcat tcatggtttg aatttgtaca
      481 aaataaaaat aataaagaga ataagttatc tatacagttt atcaacaaaa atgattatcg
      541 aattttttca tttgatgcac gagatagtcg ccatttgtca tttatagagg cgccaatcat
      601 tgtgaatgtt caggcatcag atttatcgaa tgatttttat tatgccatga tcagtcaagg
      661 cgggtattta ttcaaaaatt atgacgcgct agtaaaaaat attgaaaatt atcatcttga
      721 tggggaaatc agtggaataa ccaattataa agatagcgtg atggaaatgt atcatgaaaa
      781 caatttgaaa ttaacagtac tcaacttttc acaaatcatt atcgcaatca ttttaataat
      841 tattatttta tttgatgtga aatattattt tgaacagcat cgaaaattac tcgtaatcaa
      901 aaagctatat ggttattcaa cattaagagc caattaccaa tacttattaa taaataatat
      961 agttgttgtt tttattggaa tattgacgaa tgtaatttta cattctcagt atataatgat
     1021 gatatttgca acgattcttg tcgttcaaat attattgcag atttgcagcc tatactatca
     1081 tggccgacgt tttaatgaag ttatcaagga gttttaacta tgaaatttat cattgcaata
     1141 ttattaggat taatcgtatc cattactatt gcttttacaa tcatacatca tcctatactt
     1201 gatcgtttta atcctttctt aaaaacggag tatagttatg ccaaagtgcc aaaaggtacg
     1261 caacaatatg ttaatattac ggcttatagt gaaagagggg aaaagcttga ttataaatta
     1321 acatttaatg gattttcacc tagtagaacg tatgtcgaaa taaagcataa agggcaatat
     1381 gtcatatcga tcacatatgt tgaaaaagag gatataccaa aagaggtaag acaagaatga
     1441 tagaattaaa agatttaacc atacaaaaag gtaatataca tattttgaag aaacttaatt
     1501 tgaaatttca atgtggaaaa tcatatgcac ttattggtaa aagtggatgc ggtaaatcta
     1561 cattattaaa tactattgct ggacttgaaa aaacggggaa acaatatgtc tattttaatg
     1621 gtcaattaga ac
//
LOCUS       DAHHSC010000311         1600 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.318, whole genome shotgun sequence.
ACCESSION   DAHHSC010000311 DAHHSC010000000
VERSION     DAHHSC010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1600)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1600)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1600
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.318"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            complement(234..1442)
                     /locus_tag="NBI57_002625"
     CDS             complement(234..1442)
                     /locus_tag="NBI57_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HCD1823543.1"
                     /translation="MNENEKNIRKNFLKFTESRYMKFVGGNDLVFSLIALVLLGIVIF
                     IFEKVSYVFDPFIIVFKTIAAPIIVSLILFYLFNPIVNMMERYRIPRVAGISIIYLAV
                     VGVITLIVNLLIPIIGSQVDSLVKNSPQYLEKLINSIDKIANNTFFSSYYSQINDWLN
                     SLPKKIPSMLSEFTDGFGSKIATFAETIANIGVVIVTTPFVLFFMLKDGHHFKEFSTN
                     IMPPKFRKDFHDLLEKMSVQVGSYIQGQIIVSFCIGILLFIGYSVIGLKYSLVLASIA
                     AVTSVVPYLGPTIAISPAIVIAAITSPWMLLKLAVVWTLVQFVEGHFISPNIMGKTLK
                     IHPLTIIFILLCAGKLLGIVGVILGIPGYAILKVLVTHLFQLFKRRYNRFYGNDVGEY
                     DIKESNKIVE"
BASE COUNT          604 a          275 c          206 g          515 t
ORIGIN      
        1 tgagtgcttc ttaatatatt aaaaatacca cttattttta ttttcgacat aataatgaag
       61 tactaatcaa gtggcaaata ctcgtttcat tatgcatgtg ctttataaac ggctaaactt
      121 tcgtaaaaat ttataagcta aacatgtgct gataatcata aatgtaactg cccaacatta
      181 taagcaccat aaaaaatacc aacaaacata tagtcattgg tattttttat tacttattca
      241 actattttat tactttcttt aatatcatat tcacctacat cattaccata gaaacgattg
      301 tatcgacgtt taaataattg gaataaatga gtaactaata cttttaaaat agcatatccc
      361 ggaataccta aaataacgcc tacaatacca agcaatttgc ctgcacacag taaaatgaaa
      421 atgattgtaa gtggatgaat cttaagtgtt ttacccatga tatttggtga aatgaagtgc
      481 ccttcaacaa attgtactaa agtccatact actgctaatt ttaagagcat ccacggcgat
      541 gttatagcag ctattacaat agctggagaa atcgctatag taggccctaa atatggtaca
      601 acacttgtaa ctgccgcaat actagctaat actaagctat atttcaaccc gataaccgaa
      661 taaccgataa acaacagtat accgatacag aatgaaacga taatttgtcc ttgaatgtat
      721 gaaccaactt gaacactcat tttttcaagt agatcatgaa aatcttttcg gaatttcggt
      781 ggcataatat tcgttgaaaa ttctttgaag tgatgtccat ctttaagcat aaagaatagt
      841 acaaatggtg ttgtgacaat cacaacgcca atattagcaa tcgtttctgc aaacgttgca
      901 attttagacc caaagccatc tgtaaattca cttaacatag atggtatttt cttaggtaaa
      961 gaatttaacc aatcattaat ttgactataa tacgaagaga aaaacgtatt atttgctatt
     1021 ttatcaatag aattaattaa tttttctaga tattgcggtg aatttttaac taaactatct
     1081 acttgcgaac caataatagg tatcaataaa ttaacaatta acgtaataac acctactaca
     1141 gctagataaa taatagaaat acctgcaact cttggtattc tataacgttc catcatattt
     1201 acgattgggt taaatagata gaatagaatt aaagagacga tgataggtgc tgctatcgtc
     1261 ttaaaaacga tgataaaagg atcaaaaaca tatgatactt tttcgaaaat aaaaataaca
     1321 atacccaaca atactagcgc tattaatgag aagactaaat cattcccacc aacaaacttc
     1381 atataccgtg attcggtaaa ttttaaaaaa ttctttctta tattcttttc attttcattc
     1441 aattatagat tcaccaccac ttaaaatatt cataagatgt tgtataaaaa cagtccactt
     1501 atgtaaaatt actaacatca atgataccat acaaataccc tttacagtaa cgaaacgccc
     1561 gtgtaataca cttaatttag gattgattaa tcctttcttc
//
LOCUS       DAHHSC010000312         1589 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.319, whole genome shotgun sequence.
ACCESSION   DAHHSC010000312 DAHHSC010000000
VERSION     DAHHSC010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1589)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1589)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1589
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.319"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            <1..1504
                     /gene="vraG"
                     /locus_tag="NBI57_002626"
     CDS             <1..1504
                     /gene="vraG"
                     /locus_tag="NBI57_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease VraG"
                     /protein_id="HCD1823544.1"
                     /translation="LLMIVFTLLGIKEKVPIIFSLRAVFETLMLIGVAYFLTSAQNFI
                     LVFKQSISQMSKNNQVKETNHNKITFEEVVLGILGIVLITTGYYLSLNIVQYYDSIGI
                     LMFILLSTVIGAYLFFKSSVSLVFKMVKKFRKGVISVNDVMFSSSIMYRIKKNAFSLT
                     VMAIISAITVSVLCFAAISRASLSSEIKYTAPHDVTIKDQQKANQLASELNNQKIPHF
                     YNYKEVIHTKLYKDNLFDVKAKEPYNVTITSDKYIPNTDLKRGQADLFVAEGSIKDLV
                     KHKKHGKAIIGTKKHHVNIKLRKDINKIYFMTDVDLGGPTFVLNDKDYQEIRKYTKAK
                     HIVSQFGFDLKHKKDALALEKVKNKVDKSIKTRSEAISSISSLTGILLFVTSFLGITF
                     LIAVCCIIYIKQIDETEDELENYSILRKLGFTQKDMARGLKFKIMFNFGLPLVIALSH
                     AYFTSLAYMKLMGTTNQIPVFIVMGLYICMYAVFAVTAYNHSKRTIRHSI"
BASE COUNT          585 a          196 c          265 g          543 t
ORIGIN      
        1 gttattaatg attgtcttta cattattagg aattaaagaa aaggttccaa ttatttttag
       61 tttgagggcg gtatttgaaa cattaatgtt aatcggtgtc gcttattttt taacctctgc
      121 tcaaaatttt atattagtgt tcaaacaatc tatttcacag atgtcaaaga ataaccaggt
      181 taaagaaaca aatcataata aaattacatt tgaagaggtt gttttaggca tcttaggtat
      241 agtattgatt accacaggat actatctatc tttgaacatt gttcaatatt atgattctat
      301 cggtatactt atgtttattt tattgtcaac tgtgattggg gcatacttat tttttaaaag
      361 ctctgtttct ctagttttta aaatggtgaa gaagtttaga aaaggtgtta taagtgtaaa
      421 tgatgtcatg ttctcatcat ctattatgta tcgtattaag aaaaatgctt tttcacttac
      481 ggtcatggca atcatttcag cgattactgt ttcagttctt tgctttgctg ctataagtag
      541 agcgtcctta tcaagtgaaa taaaatatac tgcaccacac gacgttacaa ttaaagacca
      601 acaaaaagct aatcaattag caagtgaatt aaacaatcaa aaaattcctc atttttataa
      661 ttataaagaa gtaattcata cgaaattgta taaagataat ttatttgatg taaaagcgaa
      721 agaaccatac aatgtaacaa ttactagtga taaatatatc cctaatactg atttgaaacg
      781 tggacaagct gatttgtttg tagcggaagg ttctatcaaa gatttagtga aacataagaa
      841 gcatggtaag gcaattatag gaacgaaaaa acatcatgtt aatattaagt tacggaaaga
      901 tattaataaa atctatttta tgacagatgt tgatttaggt ggaccaacgt ttgtcttaaa
      961 tgacaaagac tatcaagaaa taagaaagta tacaaaagca aagcatatcg tctctcaatt
     1021 tggattcgat ttgaaacata aaaaagatgc tttagcatta gaaaaagtga aaaataaagt
     1081 tgataaatct attaaaacaa gaagtgaagc gataagctca atatcaagtt taaccggaat
     1141 attattattt gtaacatcat ttttaggtat tacattcttg attgctgtat gttgcattat
     1201 atacattaag caaatagatg aaaccgaaga tgagttagag aattatagta tattgagaaa
     1261 gcttggattt acacaaaaag atatggcaag gggactaaag tttaaaatta tgtttaattt
     1321 tgggttacct ttagttattg cactatcaca tgcatatttt acatcattag catatatgaa
     1381 attaatgggt acaacgaatc aaataccggt tttcatagta atgggattat acatttgtat
     1441 gtatgctgtt tttgcagtga cggcttataa tcattccaag cgaacaatta gacattccat
     1501 ataaaatata cagatggctt tcagtagagt agtggattcg aattcacgaa ctatactgga
     1561 agctttttat tataaatgaa gagaagtta
//
LOCUS       DAHHSC010000313         1585 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.320, whole genome shotgun sequence.
ACCESSION   DAHHSC010000313 DAHHSC010000000
VERSION     DAHHSC010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1585)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1585)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1585
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.320"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            complement(347..1540)
                     /gene="fmtA"
                     /locus_tag="NBI57_002627"
     CDS             complement(347..1540)
                     /gene="fmtA"
                     /locus_tag="NBI57_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala esterase FmtA"
                     /protein_id="HCD1823545.1"
                     /translation="MKFNKVKLVIHACVLLFIIISIALIFHRLQTKTHSIDPIHKETK
                     LSDNEKYLVDRNKEKVAPSKLKEVYNSKDPKYKKIDKYLQSSLFNGSVAIYENGKLKM
                     SKGYGYQDFEKGIKNTPNTMFLIGSAQKFSTGLLLKQLEEEHKININDPVSKYLPWFK
                     TSKPIPLKDLMLHQSGLYKYKSSKDYKNLDQAVKAIQKRGIDPKKYKKHMYNDGNYLV
                     LAKVIEEVTGKSYAENYYTKIGDPLKLQHTAFYDEQPFKKYLAKGYAYNSTGLSFLRP
                     NILDQYYGAGNLYMTPTDMGKLITQIQQYKLFSPKITNPLLHEFGTKQYPDEYRYGFY
                     AKPTLNRLNGGFFGQVFTVYYNDKYVVVLALNVKGNNEVRIKHIYNDILKQNKPYNTK
                     GVIVQ"
BASE COUNT          500 a          258 c          193 g          634 t
ORIGIN      
        1 gataatctca atgttgttca cgtcaataaa attatcccta ggtttttaaa aattgtacat
       61 gtttaaacaa tcaaaagtgt acattattaa attatcattt ccagttagat ttagaaaaca
      121 ttcacaccac gcatggacca acgtattcgt cttcattcat tttagtctga tgccaactca
      181 ccaaatgatt tcccctttta tcagttaaca catattttta aactcatcct tcttcaacct
      241 tatactttaa actttttacc actataaaaa aaaagatata actcgtatac tggtcagata
      301 aggtcgatgc cttaatgcca tatacatgtt atatcttcta tattaattat tgaacaataa
      361 cacccttcgt attgtaaggt ttattttgtt ttaaaatatc attataaata tgtttgattc
      421 gaacttcatt gtttcctttt acatttaatg caagtacaac tacatactta tcattatagt
      481 aaacagtaaa gacttgtcca aagaatcccc cgttaagtct attcaatgtt ggcttagcat
      541 agaaaccata tcgatattca tctggatact gtttcgtacc aaactcatgt aataatggat
      601 tggttatttt aggactgaat aatttatatt gttgtatttg agtaattaat ttacccatat
      661 ctgttggtgt catatataaa ttacctgcac catagtattg gtccaaaata ttaggtctta
      721 agaatgaaag tcctgtacta ttataagcat aaccttttgc tagatatttt ttaaaaggtt
      781 gttcatcata aaatgctgtg tgctgaagtt ttaaaggatc tcctattttt gtataataat
      841 tctcagcata agatttacct gtaacttctt caataacttt cgcaagtact aaataattcc
      901 catcgttata catatgcttt ttgtatttct taggatcaat accacgtttt tgaatcgctt
      961 taactgcttg atctaaattt ttataatctt ttgaggattt atatttatat aatccacttt
     1021 gatgcaacat taaatctttc aatgggatag gcttagatgt tttaaaccat ggaaggtatt
     1081 tacttactgg atcattgata tttattttat gttcttcttc taactgtttt aacagtaacc
     1141 ctgttgaaaa tttttgagct gaacctatta aaaacatcgt attcggtgtg tttttaatac
     1201 ctttttcaaa atcttgatat ccataacctt tactcatttt caatttgcca ttttcatata
     1261 tagctactga accgttaaat aatgaacttt gtaaatactt gtcaattttc ttatatttag
     1321 gatccttgct attatatacc tcttttagtt tagacggcgc aaccttttcc ttattacgat
     1381 ccactaaata tttttcattg tctgataatt ttgtttcctt atgtattggg tctatagaat
     1441 gtgtcttcgt ctgtaatcga tgaaaaatta acgctataga aataatgata aataatagta
     1501 cacacgcatg tataactagt tttactttat taaatttcat ttgataattg cctcacaatg
     1561 tattcatttt atatatttaa atcgt
//
LOCUS       DAHHSC010000314         1562 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.321, whole genome shotgun sequence.
ACCESSION   DAHHSC010000314 DAHHSC010000000
VERSION     DAHHSC010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1562)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1562)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1562
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.321"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            63..1289
                     /gene="ftsW"
                     /locus_tag="NBI57_002628"
     CDS             63..1289
                     /gene="ftsW"
                     /locus_tag="NBI57_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsW"
                     /protein_id="HCD1823546.1"
                     /translation="MKNFRSILRYIGKTSKFIDYPLLVTYIVLSLIGLVMVYSASMVP
                     ATKGTLTGGIDVPGTYFYNRQLAYVIMSFIIVFFIAFLMNVKLLSNIKVQKGMIITIV
                     SLLLLTLVIGKDINGSKSWINLGFMNLQASELLKIAIILYIPFMISKKMPRVLSKPKL
                     ILSPIVLALGCTFLVFLQKDVGQTLLILIILVAIIFYSGIGVNKVLRFGIPAVLGFLV
                     VFVIALMAGWLPSYLTARFSTLTDPFQFESGTGYHISNSLLAIGNGGVFGKGLGNSAM
                     KLGYLPEPHTDFIFAIICEELGLIGGLLVITLEFFIVYRAFQFANKTSSYFYKLVCVG
                     IATYFGSQTFVNIGGISATIPLTGVPLPFISFGGSSMISLSIAMGLLLIVGKQIKVDQ
                     QRKKQQQKVDIRRQFN"
BASE COUNT          541 a          199 c          274 g          548 t
ORIGIN      
        1 aaaattaaag tataaaacga aatcatttga agtataaatt gtagaattgg atgactgaat
       61 atatgaagaa ttttagaagt attttacggt atattggtaa aacctcaaag tttattgatt
      121 atccgttatt agttacatat attgtattga gtttaattgg attagtgatg gtatatagtg
      181 caagtatggt tccagcaact aaaggcacat tgactggtgg tatcgatgtt ccaggaacgt
      241 atttttacaa ccgacaatta gcatatgtca taatgagttt tataattgta ttttttattg
      301 catttttaat gaatgttaaa ttactgagta atattaaagt gcaaaaaggt atgattataa
      361 ctatcgtctc actattatta ctgacgttag taataggtaa agatattaat ggttctaaaa
      421 gttggataaa cttaggattt atgaacttac aggcatctga gttattaaaa attgcaatca
      481 tattatatat cccatttatg atcagtaaaa aaatgcctag agtattaagt aaaccaaaat
      541 taattttaag tcctattgta ttagcattag gttgtacgtt tttagttttc ttacaaaaag
      601 acgtagggca aacattacta atattaatta ttttagttgc gattattttt tattcaggaa
      661 ttggggtaaa caaagtccta agatttggta taccagcagt gctaggattc ttagtagtat
      721 ttgtcattgc attaatggct ggttggttac caagctattt aactgccaga tttagtacgc
      781 taacagatcc attccaattc gaatcaggaa ctggatacca tatttccaat tcattgcttg
      841 cgataggtaa cggtggcgta tttggaaaag gattaggaaa tagtgcaatg aaattgggct
      901 atttaccaga accacataca gattttattt ttgcaattat ttgcgaagaa ttaggtttaa
      961 tcggaggatt gctagttatt actttagagt tctttattgt atatcgtgcc ttccagtttg
     1021 caaataaaac atcatcatat ttttataaac ttgtgtgtgt tgggattgcc acatactttg
     1081 gaagtcaaac gtttgtaaac attggcggta tttcggcaac aattccatta actggtgtgc
     1141 cattgccatt tatcagcttt ggtggatcat caatgattag tttaagtatt gctatgggat
     1201 tacttctgat tgtaggtaaa caaatcaaag tagaccagca acgaaagaaa caacaacaaa
     1261 aagttgatat aagaagacaa tttaattaaa aaaatactag ccaatattta gtacgagatg
     1321 acttctgtca ttaagcaatg ctaaacattg gctatttata tactctttgt caaattggac
     1381 tgatgagttc aataacggaa gttaggcaac caaacaacgc ttaacttcct ttttgttttg
     1441 atattacgag attactcatg attatcaaat tttataaaca aaacaacgta taattataac
     1501 taaattttat gactaaaaat tactaaatgt ggtggcgaaa ttagaatcat gatgataaga
     1561 ta
//
LOCUS       DAHHSC010000315         1550 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.322, whole genome shotgun sequence.
ACCESSION   DAHHSC010000315 DAHHSC010000000
VERSION     DAHHSC010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1550)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1550)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1550
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.322"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            complement(<1..419)
                     /locus_tag="NBI57_002629"
     CDS             complement(<1..419)
                     /locus_tag="NBI57_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002455976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1823547.1"
                     /translation="MKSKKRMERLYDDLFSTNFLINYGYFDITPKNNQDLTELNSYGK
                     SDAFYLKIFNMSKLAEYITYNPFYIKKFFNDRNIRSTEPIIFTIPKNNTSRREYKIPN
                     IYSYLNLMFFMQENKKEFKEVFLTNKFSTSKFFSLPD"
     gene            complement(960..1532)
                     /locus_tag="NBI57_002630"
     CDS             complement(960..1532)
                     /locus_tag="NBI57_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000244613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1823548.1"
                     /translation="MVLTSTALLFSSFEPVVHAAENKEVVKTVENDNVEFNELLEGQE
                     YIWEVLSRDDEGFKMFLDEQRAFNPNFDNDVLLAKQEGALNSSVFANNSMTLNSNKPT
                     PRGPIGATLKAIKALSPSLRHGGTAISWIIKPLSRKHAVLVKKYSRKMSNAIDRLDKS
                     TKSAVENAFVKAGIPRSDAKTLTYIVFLVI"
BASE COUNT          565 a          236 c          180 g          569 t
ORIGIN      
        1 aaatctggaa gtgaaaaaaa tttagatgtt gaaaatttat tggttagaaa aacttcttta
       61 aattcttttt tattttcttg catgaaaaac atcaaattta aatagctata aatattagga
      121 atcttatatt ctcttcttga tgtattattt ttcggaatag taaatattat tggttcagta
      181 ctacgtatgt ttctatcatt aaaaaatttt ttaatgtaaa atggattata agtaatatat
      241 tcagctaatt tactcatatt aaatattttc aaataaaatg catctgattt accgtagctg
      301 tttaattctg ttaaatcttg attattttta ggagtaatat caaaataacc ataattaatt
      361 aaaaaattag tactaaataa atcatcgtac aatctttcca ttcttttttt actcttcata
      421 gcctttctcc tacctttgca tttctctact aaccagtgtt ataatttata taacgaaaat
      481 gattaacaat taatgcacct cctctcttcg tcgattgacg cctgagagat aggcgactct
      541 attattatac catctgtgaa tatacaagca tatctacttc cgataagtcg tcttatgtta
      601 tataacttcg ttttttatag tatcaagaca aaagaagaaa cgcagttcaa aatcatcacc
      661 taaattcatt aatctaaaga aaaaacggtg taataaatag tgaatattat agtctattaa
      721 agaatagatt taaaaacact ctaaaaggtg cattaaagat ttgtaaaact ttaatatatc
      781 ttttttaggt gaaaacttat gataattgta ggttctctca ttatctatga ttattaattt
      841 taactgtatc tagaaaatca tcaccccaat cacaaattga aaatactact ttatcaagat
      901 aagaaaaaaa aaacttgccc taagatttta taaaatctta gggcaagtca gctcaatatc
      961 taaataacta aaaatacaat ataagttaaa gttttcgcat cacttcttgg tatacctgct
     1021 ttaacaaaag cattttcaac agctgatttc gtagacttat ctaacctatc aattgcatta
     1081 gacatctttc ttgaatattt ttttactaga acagcatgtt ttctacttaa tggtttaatg
     1141 atccaagata tagcagttcc accatgtctc aaagaaggtg atagagcctt aatagctttt
     1201 aaagttgcac cgataggacc tctaggagta ggcttatttg agtttaaagt catagaatta
     1261 ttagcaaata ctgaagaatt taaagcacct tcttgttttg ctagtaaaac atcattatca
     1321 aaattaggat taaaagctct ttgctcatct agaaacattt taaatccttc atcatctcta
     1381 gataaaactt cccaaatata ttcttgtcct tctaacaatt cattgaattc tacattatca
     1441 ttttctactg ttttaacaac ttccttattt tctgcagcat gaactacagg ttcaaaacta
     1501 gaaaataata atgcagtaga tgttagtacc aaagtatttt taataaaaaa
//
LOCUS       DAHHSC010000316         1527 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.323, whole genome shotgun sequence.
ACCESSION   DAHHSC010000316 DAHHSC010000000
VERSION     DAHHSC010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1527)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1527)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1527
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.323"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            46..519
                     /locus_tag="NBI57_002631"
     CDS             46..519
                     /locus_tag="NBI57_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HCD1823549.1"
                     /translation="MGLLDIASIRSIERGFNYYQSECVINLKSFSETQHEAEVKGSGN
                     KVYRCYIDMEHPRKSKCNCPHADGRRVICKHMIALLFTASPEAANKHIMMLNEVEEDY
                     QLRRNMWIDSLKEMINDMSEEELRDAYLNMLIEHGEMAELFGLDEEEEMFEDEFY"
     gene            complement(567..1334)
                     /locus_tag="NBI57_002632"
     CDS             complement(567..1334)
                     /locus_tag="NBI57_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HCD1823550.1"
                     /translation="MRFVFDIDGTLCFDGRLIDQTIIDTLLQLQHDGHELIFASARPI
                     RDLLPVLPSVFHQHTLIGANGAMISQQSKISVIKPIHTDTYHHILKIIQKYELDYIID
                     DDWNYAAQLDAENAIFERLDPHKLASCIDVANIDTPIKIILLNIDPAQITTILDELNK
                     YHQELEMIHHSNEYNIDITAQNINKYTALQYIFDADVKYIAFGNDHNDIFMLQHASSG
                     YIIGPSEAYTHAILKLDKIKHINNNTQAICKVLKSYK"
BASE COUNT          525 a          206 c          270 g          526 t
ORIGIN      
        1 tttattttct ttatttaaaa ataataataa aaaggagtat cattaatggg attacttgat
       61 attgcaagta ttcgttctat agaaagaggc tttaattatt atcaaagtga atgcgtcatt
      121 aacttaaaat cattttcaga aacgcagcat gaggctgaag taaagggcag tggcaacaaa
      181 gtatatcgtt gttatattga tatggaacat cctagaaaat ccaaatgtaa ttgtcctcat
      241 gctgatggaa gacgagtgat atgtaaacat atgattgcat tactctttac agctagtcca
      301 gaagcagcaa ataaacatat aatgatgtta aacgaagttg aagaagacta tcaattacgc
      361 agaaatatgt ggattgatag tcttaaagaa atgattaatg atatgagtga agaagaactc
      421 cgcgatgcat atttaaacat gttaattgaa catggagaaa tggcagaatt atttggatta
      481 gatgaagagg aagaaatgtt cgaggacgaa ttttattaaa atagccccta tcgattgata
      541 atgattatca tttgataggg gtgcttttat ttatatgatt ttaagacttt gcaaatagct
      601 tgtgtattat tattgatgtg tttgatttta tcaagtttca atattgcgtg tgtgtatgct
      661 tctgatggtc ctataatata gccactacta gcatgttgta acatgaaaat atcattgtgg
      721 tcattaccaa atgctatata tttaacatct gcatcaaata tatattgtaa tgcagtatat
      781 ttattaatat tttgcgctgt tatatcaatg ttatactcat ttgaatggtg aatcatttcc
      841 aattcttgat ggtatttatt tagctcgtct aatatagttg taatttgtgc cgggtctata
      901 tttaataaaa tgatcttgat tggtgtgtcg atatttgcaa catcaataca actggccagc
      961 ttatgtggat ctaaacgctc aaaaatcgcg ttctcagcgt caagttgtgc agcataattc
     1021 caatcatcat caataatata atctaactca tacttttgaa ttatttttaa gatatggtga
     1081 tatgtatctg tatgaattgg tttgataaca gaaatctttg attgctgtga aatcatagca
     1141 ccatttgcgc caattaatgt gtgctgatga aatactgatg gtaaaactgg caacaaatca
     1201 cgaatcggac gtgctgatgc aaatataagt tcatgaccat catgttgtaa ttgtaacaat
     1261 gtgtcaataa tagtctggtc aattaatcgg ccgtcaaaac aaagcgtacc atcaatatca
     1321 aagacaaatc tcatcaaatc actccaaaca atataatact atgattatag cataagtaag
     1381 ttatgtgacg atattagata atgattatga tgtagggtac cttttgcgtc acacacatta
     1441 cttcctataa tatatattat gtaaactaga atgtgaaatt ccataagggg acttatataa
     1501 cttcgatttt gtataagtcg ttttgat
//
LOCUS       DAHHSC010000317         1504 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.324, whole genome shotgun sequence.
ACCESSION   DAHHSC010000317 DAHHSC010000000
VERSION     DAHHSC010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1504
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.324"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            61..597
                     /gene="amaP"
                     /locus_tag="NBI57_002633"
     CDS             61..597
                     /gene="amaP"
                     /locus_tag="NBI57_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HCD1823551.1"
                     /translation="MKRLKNFILGLLIVVIVGFLLFMYIQDGRITEYQDYFLQFEWFQ
                     PLLISLAALLILIGLILVFSIFKPTHRKPGLYKDFDDGHVYVSRKAVEKTAFDTVAKY
                     DQVRQPNVVAKLYNKKNKSYIDIKTDFFVPNNVQVQSLTEAIRSDIKKNVEYFTEMPV
                     RKLEVNVRDQKTSGPRVL"
     gene            610..849
                     /locus_tag="NBI57_002634"
     CDS             610..849
                     /locus_tag="NBI57_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HCD1823552.1"
                     /translation="MANNHNQNGQDSTQQVINFLKVFKWRIVGFLAFLLIAILFLTLG
                     FWKTVLIIVLCLIGVGIGYMKDRKQDFMNFLNRWS"
     gene            912..1421
                     /locus_tag="NBI57_002635"
     CDS             912..1421
                     /locus_tag="NBI57_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HCD1823553.1"
                     /translation="MTVDNNKAKQAYDNQTGVNEKEREERQKQQEQNQEPQFKNKLTF
                     SDEVVEKIAGIAAREVKGILDMKGGLTDTFTNAFSSGNNVTQGVSVEVGEKQAAVDLK
                     VILEYGESAPKIFRKVTELVKEQVKYITGLDVVEVNMQVDDVMTQKEWKQKHEKNNEN
                     NNQERQGLQ"
BASE COUNT          572 a          202 c          266 g          464 t
ORIGIN      
        1 tgatgggtac atatttaata tgtttttaaa ttaactttaa ctaaggaggt gtcgttaggg
       61 gtgaaacgac ttaaaaactt tatccttggc ttattaatag tagtaatcgt ggggttcctg
      121 ttattcatgt atatccaaga tggacgtata actgaatatc aagactactt ccttcaattt
      181 gaatggttcc aaccattact tatttcactt gctgcactat tgatattaat aggtcttata
      241 ttagtattta gtatcttcaa acctacgcat cgaaaacctg gattatacaa agacttcgat
      301 gatggtcatg tttacgtatc tcgtaaagct gttgaaaaga cggcattcga tacggttgca
      361 aaatatgatc aagtaagaca accaaatgta gttgcgaaac tatataacaa aaaaaataaa
      421 tcttatatcg acattaaaac tgactttttc gtaccaaata atgttcaagt tcaatcttta
      481 actgaagcaa ttcgttcgga tattaaaaag aatgtagaat atttcacaga aatgccagta
      541 cgtaagttag aagttaatgt gagagatcag aaaacttctg gtccacgagt gttgtaaggg
      601 aggtatatca tggctaacaa tcataaccaa aacggacaag actctacaca acaagtgatt
      661 aattttttaa aagtatttaa atggagaatc gttggtttcc tagcgtttct attaatcgcg
      721 atattattct taacgttagg tttttggaaa acagtactta tcatcgtttt atgtttaatt
      781 ggtgtaggta ttgggtatat gaaagaccgt aagcaagatt ttatgaattt tttaaatcga
      841 tggagttaat tcatcaattt agaatttaat taaaaaaata taaaaattaa caagtaaaag
      901 gagaatctat tatgactgta gataacaata aagcaaaaca agcatacgac aatcaaactg
      961 gtgttaacga aaaagaaaga gaagagcgtc aaaaacaaca agaacaaaat caagagcctc
     1021 aattcaaaaa taaattaact ttctctgatg aagttgttga aaaaattgct ggtatcgctg
     1081 cacgtgaagt taaaggtatc ttagacatga aaggtggctt aactgataca ttcactaatg
     1141 cattctcaag tggaaataac gttactcaag gtgtatctgt tgaagttggt gaaaaacaag
     1201 ctgctgtaga cttaaaagta attttagaat atggtgaatc agcaccaaaa atcttccgta
     1261 aagtaactga attagttaaa gaacaagtta aatatattac tggtttagat gttgttgaag
     1321 ttaacatgca agttgatgat gtaatgactc aaaaagaatg gaaacaaaaa cacgaaaaaa
     1381 ataatgaaaa caataaccaa gaaagacaag gtttacaata agttattcga taaaaaagct
     1441 cggcacacca ttttagtgcc gagctttttt gtgattttta ataatttgaa tatggaaaat
     1501 gtat
//
LOCUS       DAHHSC010000318         1491 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.325, whole genome shotgun sequence.
ACCESSION   DAHHSC010000318 DAHHSC010000000
VERSION     DAHHSC010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1491)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1491)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1491
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.325"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            4..474
                     /gene="sigS"
                     /locus_tag="NBI57_002636"
     CDS             4..474
                     /gene="sigS"
                     /locus_tag="NBI57_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor SigS"
                     /protein_id="HCD1823554.1"
                     /translation="MKFNDVYNKHHKIIHHLLKKYNISYNYDEYYQLLLIKMWQLSQI
                     YKPSSKQSLSSFLFTRLNYYLIDLFRQQNQLKDVILCENNSPTLTEQPTYFNEHDLRL
                     QDIFKLLNHRERLWLKLYLEGYKQFEIAEIMSLSLSTIKLIKMSVKRKCQHNFN"
     gene            587..1030
                     /locus_tag="NBI57_002637"
     CDS             587..1030
                     /locus_tag="NBI57_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComK"
                     /protein_id="HCD1823555.1"
                     /translation="MQDNSTKYLLYIQTATSNHLETNCVFLHCDYILKVPINKLVSYY
                     AKLHLSSQSVLIETAKNILNINKLVPIYINPKTILFPLKHKRAPIQIYINAHYIVGMT
                     AIENSTLIHFQEGIQLEVDEPFSLVSKKCHESLALKHFIENTISN"
     gene            complement(1017..1460)
                     /locus_tag="NBI57_002638"
     CDS             complement(1017..1460)
                     /locus_tag="NBI57_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1823556.1"
                     /translation="MNKASFDKKVKKQLWFLNKKEKQALDQRLSSISDDDSVNLNKPV
                     TFANAYLRQNVFRNKETKSYSMFVTLVVMMFAYVALLGLFLFGLITSLSGVQFFVSPK
                     VDLSTTVVILTIIGAILLMIVSIYFIKIVTSYFTKKLLEIKFNSK"
BASE COUNT          560 a          249 c          169 g          513 t
ORIGIN      
        1 tacttgaaat ttaatgacgt atacaacaaa caccacaaaa tcatacacca tcttttaaaa
       61 aaatataata ttagctataa ttatgatgag tattatcaac tactcttgat aaaaatgtgg
      121 caattgagtc agatatataa accctcaagc aagcaatctt tatcctcttt tttattcact
      181 cgattaaatt attaccttat cgatttattc agacaacaaa atcaattaaa agatgtcatt
      241 ttatgtgaga ataattcacc aacattaact gaacaaccaa catactttaa tgaacatgac
      301 cttcgtttac aagatatctt caagctttta aatcatagag aaagactatg gctcaaacta
      361 taccttgaag gatacaagca atttgaaatt gctgaaatca tgtcattatc gctttcaacg
      421 attaaattaa ttaagatgtc cgttaagcgt aaatgccaac ataattttaa ttagtttatt
      481 gtgaaatttc ggtcgatgtg ttagtgatgt tgaaacaccc taacatcttc atataaattt
      541 attttaacca ttaaattaac aacttattat acgaggagta ctatatttgc aagacaattc
      601 tactaaatat ctactttata tccaaactgc tacttcaaac catctcgaaa caaattgtgt
      661 ctttttacat tgcgattaca ttcttaaagt tccaattaac aaactcgttt catattatgc
      721 gaaattgcat ttatcatcac aaagtgtgct aattgagact gcaaaaaaca tactaaatat
      781 taataaactg gttcctattt acatcaaccc aaaaactata ctttttccgt taaaacataa
      841 acgtgcaccg atacaaattt atatcaatgc acattatatt gttggtatga ctgccataga
      901 gaattcaaca ttaatacatt ttcaagaagg cattcagcta gaagttgatg agccattttc
      961 tctagtttca aaaaaatgtc atgaaagttt agctttgaag cacttcattg aaaatactat
     1021 ttcgaattaa atttaatttc taaaagtttc ttagtaaagt atgatgtcac aatttttata
     1081 aaatatatgc taacaatcat taataaaatc gctccaataa ttgttaaaat aacaacagtt
     1141 gttgataaat ctaccttagg actaacgaaa aattgtacgc cagatagact tgtaattagc
     1201 ccaaataaaa acaagcctaa taaagcaaca taagcaaaca tcatcactac taatgtaaca
     1261 aacatactat aactttttgt ttctttattt cgaaatacat tttgtcttaa gtaagcatta
     1321 gcaaaggtaa caggcttatt taagttaacg ctgtcatcat cagatataga agataatcgt
     1381 tgatctaatg cttgtttctc cttcttgttc aaaaaccata attgcttttt aactttttta
     1441 tcaaatgatg ctttattcat aattaaggat ttctctcctt tataatattg a
//
LOCUS       DAHHSC010000319         1466 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.326, whole genome shotgun sequence.
ACCESSION   DAHHSC010000319 DAHHSC010000000
VERSION     DAHHSC010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1466)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1466)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1466
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.326"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            76..>1466
                     /gene="sasG"
                     /locus_tag="NBI57_002639"
     CDS             76..>1466
                     /gene="sasG"
                     /locus_tag="NBI57_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored surface protein SasG"
                     /protein_id="HCD1823557.1"
                     /translation="MRDKKGPVNKRVDFLSNKLNKYSIRKFTVGTASILIGSLMYLGT
                     QQEAEAAENNIENPTTLKDNVQSKEVKIEEVTNKDTAPQGVEAKSEVTSNKDTIEHEA
                     SVKAEDISKKEDTPKEVANVAEVQPKSSVTHNAEAPKVRKARSVDEGSFDITRDSKNV
                     VESTPITIQGKEHFEGYGSVDIQKNPTDLGVSEVTRFNVGNESNGLIGALQLKNKIDF
                     SKDFNFKVRVANNHQSNTTGADGWGFLFSKGNAEEYLTNGGILGDKGLVNSGGFKIDT
                     GYIYTSSMDKTEKQAGQGYRGYGAFVKNDSSGNSQMVGENIDKSKTNFLNYADNSTNT
                     SDGKFHGQRLNDVILTYVASTGKMRAEYAGKTWETSITDLGLSKNQAYNFLITSSQRW
                     GLNQGINANGWMRTDLKGSEFTFTPEAPKTITELEKKVEEIPFKKERKFNPDLAPGTE
                     KVTREGQKGEKTIT"
BASE COUNT          598 a          194 c          295 g          379 t
ORIGIN      
        1 ctcctattgt atttgtaaaa ttacatgtaa attcactgta agtaaagtgg aaaatatgga
       61 aaaaggagta tgcaaatgag agataagaaa ggaccggtaa ataaaagagt agattttcta
      121 tcaaataaat tgaataaata ttcaataaga aaatttacag ttggaacagc atctatttta
      181 attggctcac taatgtattt gggaactcaa caagaagcag aagcagctga aaacaatatt
      241 gagaatccaa ctacattaaa agataatgtc caatcaaaag aagtgaagat tgaagaagta
      301 acaaacaaag acactgcacc acaaggtgta gaagctaaat ctgaagtaac ttcaaacaaa
      361 gacacaatcg aacatgaagc atcagtaaaa gctgaagata tatcaaaaaa ggaggataca
      421 ccaaaagaag tagctaatgt tgctgaagtt cagccgaaat cgtcagtcac tcataacgca
      481 gaggcaccta aggttagaaa agctcgttct gttgatgaag gctcttttga tattacaaga
      541 gattctaaaa atgtagttga atctacccca attacaattc aaggtaaaga acattttgaa
      601 ggttacggaa gtgttgatat acaaaaaaac ccaacagatt taggggtatc agaggtaacc
      661 aggtttaatg ttggtaatga aagtaatggt ttgataggag ctttacaatt aaaaaataaa
      721 atagatttta gtaaggattt caattttaaa gttagagtgg caaataacca tcaatcaaat
      781 accacaggtg ctgatggttg ggggttctta tttagtaaag gaaatgcaga agaatattta
      841 actaatggtg gaatccttgg ggataaaggt ctggtaaatt caggcggatt taaaattgat
      901 actggataca tttatacaag ttccatggac aaaactgaaa agcaagctgg acaaggttat
      961 agaggatacg gagcttttgt gaaaaatgac agttctggta attcacaaat ggttggagaa
     1021 aatattgata aatcaaaaac taatttttta aactatgcgg acaattcaac taatacatca
     1081 gatggaaagt ttcatgggca acgtttaaat gatgtcatct taacttatgt tgcttcaact
     1141 ggtaaaatga gagcagaata tgctggtaaa acttgggaga cttcaataac agatttaggt
     1201 ttatctaaaa atcaggcata taatttctta attacatcta gtcaaagatg gggccttaat
     1261 caagggataa atgcaaatgg ctggatgaga actgacttga aaggttcaga gtttactttt
     1321 acaccagaag cgccaaaaac aataacagaa ttagaaaaaa aagttgaaga gattccattc
     1381 aagaaagaac gtaaatttaa tccggattta gcaccaggga cagaaaaagt aacaagagaa
     1441 ggacaaaaag gtgagaagac aataac
//
LOCUS       DAHHSC010000320         1455 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.327, whole genome shotgun sequence.
ACCESSION   DAHHSC010000320 DAHHSC010000000
VERSION     DAHHSC010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1455)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1455)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1455
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.327"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            145..873
                     /locus_tag="NBI57_002640"
     CDS             145..873
                     /locus_tag="NBI57_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5067 domain-containing protein"
                     /protein_id="HCD1823558.1"
                     /translation="MKKVMGILLASTLILGACGHHQDSAKKESASHKKKENDNEELNE
                     ELKEFKSKKNMDIKIKGDTIVSDKFEAKIKEPFIINEKDEKKKYIAFKMEITAKKDDK
                     DLNPSSISHDYINITQDDKNTVNKLRDGYLLSDKKYKDWTEHNQDQIKKGKTAQAMFI
                     YELRGDGNINLNVHKYSEDKTVDSKSFKFSKLKTEDFSHRAETREEVEKKEKEFEEEY
                     KKEQEREKEKEKQKDDDHSSLDEV"
     gene            964..1211
                     /locus_tag="NBI57_002641"
                     /pseudo
     CDS             964..1211
                     /locus_tag="NBI57_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015728980.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1227..>1455
                     /locus_tag="NBI57_002642"
     CDS             1227..>1455
                     /locus_tag="NBI57_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1823559.1"
                     /translation="MNNEELEMRLLLMKQSIEQLQEELAPNLKTRDLVLLRYMYSYKE
                     INMLDSYLFQLATNKEQITKKQFKTKLENIRE"
BASE COUNT          661 a          158 c          239 g          397 t
ORIGIN      
        1 aacaatacat aggatcatta ttacaagaat tatttaatat catcttagaa tctcacaaaa
       61 tatcaacttt gtttaattac tcaaaatgat aagtcattta gagtttttaa aataaatttt
      121 tgcgaaataa aggagacatg ttaaatgaaa aaagtaatgg ggatattatt agcaagtaca
      181 cttatcttag gtgcttgtgg acatcatcaa gatagtgcaa aaaaagagag cgctagtcac
      241 aaaaagaaag aaaatgacaa tgaagaatta aatgaagaac ttaaagaatt taaaagcaaa
      301 aaaaatatgg atataaaaat taaaggcgat actattgtta gtgacaaatt tgaagctaaa
      361 ataaaagaac cgtttatcat caatgaaaaa gatgagaaaa agaaatatat cgcttttaaa
      421 atggaaatta ctgctaaaaa agacgataaa gatttaaatc catcttctat ttctcatgac
      481 tatattaata tcactcaaga tgataaaaat acagtaaata aattaagaga tggttatctt
      541 ttaagtgata aaaaatataa agattggaca gaacataacc aagatcaaat taaaaaaggc
      601 aaaactgcac aagccatgtt tatctatgag ttaagaggtg atggaaatat taatttaaat
      661 gtccataaat actcagaaga taaaacagtt gattctaaat cattcaaatt tagtaaactt
      721 aaaaccgaag atttttctca tagagcggaa acaagagaag aagtagaaaa gaaagaaaaa
      781 gaatttgaag aagagtacaa aaaagaacaa gaacgagaga aagaaaaaga aaagcaaaaa
      841 gatgacgacc acagtagttt agatgaagta taaatttggt ttgttgattg tcttacattc
      901 agttatttaa tttttagaga aactgactta tttatagctt agccaacatg atactataaa
      961 actatgatta aaaataaaaa atctgtgttt aatggtgtta aagaaatcag tccccgtgcc
     1021 ggaaaatcat aaaaaaaaga tgggataaat ttgtaaatcc cgcattgaat aatgcattaa
     1081 aacaagaaga ggctacatgg ggaaatgtac aaggtcaagt ttcacaagca ttgatgggaa
     1141 ctggtattaa agattctact gcaagaagca tagggttttg ggtttcccaa gttggtcaag
     1201 cattaatata aaaagagtgt gataaaatga ataatgaaga attagaaatg agattgttat
     1261 taatgaaaca atcaatagaa caattacaag aagaactagc gcctaactta aaaactagag
     1321 atttagtgtt attaagatat atgtattcat ataaagaaat taatatgcta gattcttact
     1381 tgttccaatt ggctacaaat aaagagcaaa taacaaaaaa acaatttaaa acaaaattgg
     1441 aaaacattag agaag
//
LOCUS       DAHHSC010000321         1442 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.328, whole genome shotgun sequence.
ACCESSION   DAHHSC010000321 DAHHSC010000000
VERSION     DAHHSC010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1442)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1442)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1442
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.328"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            complement(76..1425)
                     /gene="brnQ"
                     /locus_tag="NBI57_002643"
     CDS             complement(76..1425)
                     /gene="brnQ"
                     /locus_tag="NBI57_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HCD1823560.1"
                     /translation="MKKKLTFKENMFIGSMLFGLFFGAGNLIFPIHLGQAAGSNVFIA
                     NLGFLITAIGLPFLGIIAIGISKTSGLFEIASRVNKTYAYIFTIALYLVIGPFFALPR
                     LATTSFEIAFSPFLSPKQITLYLFIFSFVFFVIAWFFARKPSRILEYIGKFLNPVFLV
                     LLAIILLFAFIHPLGGISDAPISKQYQSHALFNGFLDGYNTLDALASLAFGIIIVATI
                     KKLGIENPTDIAKETIKSGTISIIMMGIIYTLLAIMGTLSIGHFKLSENGGIALAQIT
                     QYYLGNYGIVLLSLIVMVACLKTAIGLITAFSETFEHLFPKMNYLAIATVVSFISFLF
                     ANVGLTKIIMYSVPVLMFLYPLAIALIVLTLFSSKFHHSKIIYQCTIFFTMIAALVDG
                     LKASPEFISSTSFSQTLINFSQKYLPLSDIGMGWVVLSLIGFIIGFIIYKIKHRKIP"
BASE COUNT          538 a          225 c          249 g          430 t
ORIGIN      
        1 ttgcagttta atgtagtatg aataaaaaat gccacaagca tatgtttaac atatgactgt
       61 ggcatagtat tatgcttatg gaattttacg atgcttaatt ttataaataa tgaagccgat
      121 aatgaaacca atcaaactga gaacaaccca gcccatacca atgtctgata atggtaaata
      181 tttttggctg aaattaatca aagtttgtga gaatgatgtg cttgaaatga actctggact
      241 agcttttaat ccatctacta atgcagcaat cattgtaaag aaaatggtac attgataaat
      301 aatttttgaa tgatggaatt tgctactaaa taatgttagt acaatcaagg caattgctaa
      361 tggatataag aacattaaca ctgggactga gtacataata atcttagtta agccaacatt
      421 cgcgaataag aacgaaataa agcttacaac tgttgcaatc gctaggtaat tcattttagg
      481 gaaaaggtgt tcgaatgttt ctgaaaatgc cgtaatcaaa ccgatggctg tttttaaaca
      541 agcaaccata acgataagtg acaacaggac gataccgtag ttacctaagt agtattgcgt
      601 aatttgcgct aaggcaatac caccattttc actaagtttg aaatgaccaa tacttaatgt
      661 acccatgatt gctagtaggg tataaataat ccccatcata atgatactga tagtaccaga
      721 cttaattgtt tctttagcga tatcagttgg attttcgata cctaactttt taatcgttgc
      781 aacaatgatg ataccaaatg ccaatgacgc tagcgcatct aaggtattgt atccatctaa
      841 aaagccgtta aataaggcat gtgattgata ttgtttacta ataggtgcat cagatatgcc
      901 acctaatgga tggataaaag caaataataa aataattgct aataatacta agaataccgg
      961 atttaaaaat ttaccgatat attctaaaat tcttgatggc tttctcgcaa aaaaccatgc
     1021 aatcacaaag aagacgaagc taaaaataaa taaatataaa gtgatttgct ttggtgataa
     1081 aaatggcgaa aatgcaattt caaatgatgt cgttgccagt ctaggtaagg cgaaaaatgg
     1141 tccgataact agatataagg caatcgtgaa aatgtaagca tatgttttat taacacgcga
     1201 tgcaatttca aataaaccag atgtctttga aatgccaata gcaatgatac ctagaaatgg
     1261 taagccaatt gctgtaatta aaaatcctaa gttagcgata aaaacgttag aaccagcagc
     1321 ttgacccaag tgtattggga agataagatt gccggcacca aagaataaac caaataacat
     1381 agaacctata aacatgtttt ctttaaatgt tagtttcttc ttcatatatg tatttgttct
     1441 cc
//
LOCUS       DAHHSC010000322         1425 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.329, whole genome shotgun sequence.
ACCESSION   DAHHSC010000322 DAHHSC010000000
VERSION     DAHHSC010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1425)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1425)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1425
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.329"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            <1..889
                     /locus_tag="NBI57_002644"
     CDS             <1..889
                     /locus_tag="NBI57_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000825515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="iron-hydroxamate ABC transporter
                     substrate-binding protein"
                     /protein_id="HCD1823561.1"
                     /translation="CSLFILTACSTSVDKTSDSTKTIDYKIENGKTLKVPEKPKRVAV
                     LTGFYVGDFIKLGIKPIAVSDITKDSSILKPYLKGVDYIGENDVEKVAKAKPDLIVVD
                     AMDKNIKKYQKIAPTVPYTYNKYNHKEILKEIGNLTNNEDKAKKWIEEWEDKTRKDKK
                     EIQSKIGQATASVFEPDEKQIYIYNSTWGRGLDIVHDAFGMPMTKQYKDKLQEDKKGY
                     ASISKENISKYAGDYIFLSKPSYGKFDFEKTHTWQNIEAVKKGHVISYKAEDYWFTDP
                     ITLEHLRSKLKKEILNKSH"
     gene            947..>1425
                     /locus_tag="NBI57_002645"
     CDS             947..>1425
                     /locus_tag="NBI57_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCD1823562.1"
                     /translation="MKRLDGIPESMLIPLIARAKEYEYEKPIIKDALSKKIFDGLDDI
                     YKNVTCDDMSQIGISIRSVIIDSVTKRLIKDNKNLIVINIGCGLDTRFQRFNNEKISW
                     IDLDVPESIEIRKTFFKETDSYKMIAKSMLDYSWIEDVINYKFFNSKSNILFIFEGV"
BASE COUNT          602 a          149 c          242 g          432 t
ORIGIN      
        1 atgtagttta tttattttga ctgcgtgttc aacatctgtc gataaaacaa gtgactcgac
       61 aaagactata gactataaaa ttgaaaatgg taaaacactg aaagtaccag agaaacctaa
      121 aagagttgct gtattaactg gattttatgt tggtgatttt ataaagttag gaatcaaacc
      181 aattgctgtt tcagatataa ctaaagattc ttcaatttta aaaccttatt taaaaggggt
      241 tgattatatt ggagaaaatg atgttgaaaa agttgctaaa gcaaaacccg atttaatcgt
      301 tgtagatgct atggataaaa atattaaaaa ataccaaaaa atagcgccaa cagtcccata
      361 tacatataat aaatacaatc ataaagaaat attaaaagaa ataggcaatt tgactaataa
      421 tgaggataaa gcaaaaaaat ggattgaaga gtgggaagat aaaactagga aagataaaaa
      481 agaaattcaa agtaaaattg gtcaagcaac agcatctgtg tttgaaccag atgaaaagca
      541 aatatacata tataactcta catggggtcg tggtttggat attgttcatg atgcattcgg
      601 tatgccaatg acaaagcaat ataaagataa attacaagaa gataaaaaag gttatgcttc
      661 gatttcaaaa gaaaatatta gtaaatatgc tggtgattat atatttttaa gtaagccttc
      721 atacgggaaa tttgattttg aaaaaacaca tacatggcag aatattgaag ctgtaaaaaa
      781 aggacatgta atttcatata aagcagaaga ttattggttc acagatccta ttacattaga
      841 acatttgaga agtaaattaa aaaaagaaat tttaaataaa tctcactaga aataagttat
      901 aaaaatttct aatgtattaa taataatatt tttacggagt gattaaatga aacgactaga
      961 tggaattccc gaatcaatgt taattccttt gatagctcga gcaaaagagt acgaatacga
     1021 aaaaccaata ataaaagacg cactatctaa aaaaatattt gatggtttag atgatatata
     1081 caaaaatgtt acatgtgatg acatgtctca aattggaatt agtatacgtt ctgtgataat
     1141 agatagtgtt actaaaaggc ttatcaagga taataaaaat ttgattgtga tcaatatagg
     1201 ttgtggctta gatacaaggt ttcaaagatt taataacgaa aaaatatcgt ggatagattt
     1261 agatgtacct gaatcaatag aaatacgaaa aacatttttt aaagaaacag atagttataa
     1321 gatgatagct aaatcaatgc tggattacag ttggattgaa gatgttataa attataaatt
     1381 ttttaatagt aagtcaaata tattgtttat ctttgaaggt gtatt
//
LOCUS       DAHHSC010000323         1423 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.330, whole genome shotgun sequence.
ACCESSION   DAHHSC010000323 DAHHSC010000000
VERSION     DAHHSC010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1423)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1423)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1423
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.330"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            complement(1..714)
                     /locus_tag="NBI57_002646"
                     /pseudo
     CDS             complement(1..714)
                     /locus_tag="NBI57_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000503564.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="exotoxin"
BASE COUNT          474 a          257 c          181 g          511 t
ORIGIN      
        1 ctattgatag ttttattatc agcatacatt tttaatacct cataaccata ctcaccattt
       61 aaattataaa aatcatattc tacattttgt tcatcatcat tatgatattt aacataccct
      121 ttaacaatgt cgccaccttg ttcgtacaac ttatacttct cattcaaata atttcttaat
      181 tgaacatcta attcttgaat ggtaacaatt ttcttttttc gtttttactg cagttagttc
      241 tatttctgtt tgatgtccgt caatccataa attagaaggt atattcttat aatcatctta
      301 tttattattg tcacttaaag taacaccacc ataactacat tgcgttttat tggttgctcc
      361 cccatgacat tcatagctat agcttccagc atatatatcg atattcttat ttataaattt
      421 ctttgaaagt gcctcatttt cgaattcaac tttaaaatcc tttttcgaag caacatttac
      481 gaacatgtca tgaaataaca aatcatgctt caacagtcta tcgtttgtgt ttttattttc
      541 aagctgagtt ggattgatga atgacatctt agcattactt agacgctttg aatcaaactt
      601 acttttatga tgtaagtctg aatattcgat tgcactcgct gaatttgtaa aaattccttc
      661 taacaataat attaaaataa atcctgttaa atatttaact tcaaactccc ccaaaacaat
      721 tattaattta catttgagag cgagacataa atgataaaca ggctgggaca taaatcaatg
      781 ttctatgctc tacgaagtta tattggcagt agttgactga acgaaaatgc acttgtaaca
      841 agcttttttc aattctagtc aggggcccca acacagagaa tttcgaaaag aaattctaca
      901 ggcaatgcaa gttggggcgg ggccccaaca tagaagctgg cggaaagtca gcttacaata
      961 atgtgcaagt tgggtgggac gacgataaag aaatactttt tctatagaaa ttagtatttc
     1021 ttatacatga gttttactca tgtattccta tttttaagta cacattagct gtggctaatg
     1081 tgtaagaacc actacataat aaatcattag tggctcttta tcatttctgt cccactccct
     1141 tgagcctaca attttgatta tgtatcacgt tcataatact ttccaatttg aaataccctg
     1201 ttcgcaatta ttagttgagt tatatcaacc aatacagtgt aaacataaaa attccaaacc
     1261 gtttaacagt atatcagaaa aaatgataat catttctttt atttttatta aatttcataa
     1321 tgattcatat aatattcgca ttaaaacatc acaatactga tacacaacaa aaagagcagt
     1381 acatttgtca tgcaccactc tcaacaaata tatttttcaa tga
//
LOCUS       DAHHSC010000324         1422 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.331, whole genome shotgun sequence.
ACCESSION   DAHHSC010000324 DAHHSC010000000
VERSION     DAHHSC010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1422)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1422)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1422
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.331"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            24..1370
                     /locus_tag="NBI57_002647"
     CDS             24..1370
                     /locus_tag="NBI57_002647"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HCD1823563.1"
                     /translation="MHFIAISINHRTADVALREQVAFRDDALRIAHEDLYETKSILEN
                     VILSTCNRTEVYAVVDQIHTGRYYIQRFLARAFGFEVDDIKAMSEVKVGDEAVEHLLR
                     VTSGLDSIVLGETQILGQIRDAFFLAQSTGTTGTIFNHLFKQAITFAKRAHNETDIAD
                     NAVSVSYAAVELAKKVFGKLKSKQAIIIGAGEMSELSLLNLLGSGITDITVVNRTIEN
                     AMKLAAKHQVKYDELSSLPNLLESADIVISSTSAQSYIITNEMIERIAENRKQDSLVL
                     IDIAVPRDIEPGISAITNIFNYDVDDLKGLVDANLRERQLAAATISEQIPAEIHAHNE
                     WISMLGVVPVIRALREKAMAIQAETMDSIDRKLPGLSERERKIISKHTKSIINQMLKD
                     PIKQAKELSSDKKSNEKLELFQNIFDIEAECPHEQAKQQKESKVKEISARRIFSFE"
BASE COUNT          522 a          196 c          283 g          421 t
ORIGIN      
        1 tagtatatat gggggcgttt caaatgcatt ttattgcaat tagtataaat catcgcacag
       61 ctgatgttgc actaagagag caagttgctt ttagagatga tgccttacga attgcccatg
      121 aagatttata tgaaactaaa tctattttag aaaatgtcat attatcaaca tgtaatcgaa
      181 ctgaagtata tgctgttgtt gatcaaattc acacaggtcg ttactatatt caacgatttc
      241 tagctcgtgc atttggattt gaagtagatg atattaaagc aatgtcagaa gtaaaagtgg
      301 gggacgaagc agtagaacat ttattgcgtg tcacttctgg tttagattca atcgtacttg
      361 gagaaactca aattttaggt caaataagag atgcattttt cttagcgcaa agcacaggta
      421 cgacaggaac aatttttaat catctattta aacaggcaat tacttttgca aaaagagcac
      481 ataatgaaac agatatagct gataatgctg taagtgtgtc ttatgctgcg gtcgagttgg
      541 cgaaaaaagt atttggcaaa ttgaaaagta agcaagctat cattattggt gcaggggaaa
      601 tgagtgaatt atcactatta aatcttcttg gttctggaat tactgatatt acagtagtaa
      661 atagaacaat tgaaaatgct atgaaattag cagcaaagca tcaagtgaaa tatgatgaac
      721 tatcatcatt accaaattta cttgaaagtg cagatattgt gattagttca acgagtgcac
      781 aatcttatat cattacaaat gaaatgatag aaagaattgc agaaaataga aagcaagatt
      841 cactagtatt gattgatatt gcagttcctc gagatattga acctggtatt agtgccatca
      901 caaacatctt taattatgat gttgatgact taaaaggttt agttgatgca aacttacgtg
      961 agcgacaatt agcggctgca acaatttcgg aacaaattcc tgcagaaata catgcacaca
     1021 atgagtggat tagtatgttg ggtgttgttc cagtgattag agctttacgt gaaaaagcta
     1081 tggcaattca agcagaaacg atggacagta ttgatcgtaa attgccaggg ttaagcgaaa
     1141 gagaacgtaa aattatatct aaacatacaa aaagtatcat caatcaaatg ttgaaagatc
     1201 ctattaaaca ggccaaagaa ttaagtagtg ataagaaaag taatgaaaaa ttagagctat
     1261 ttcaaaatat atttgatatc gaagctgaat gtcctcatga gcaagcgaag caacaaaaag
     1321 aaagcaaggt caaggaaatt tcagcgcgac gtatctttag ttttgaataa gcatattaat
     1381 atggtgataa tatgcaagaa aacctgttta ttcgattcaa tg
//
LOCUS       DAHHSC010000325         1379 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.332, whole genome shotgun sequence.
ACCESSION   DAHHSC010000325 DAHHSC010000000
VERSION     DAHHSC010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1379)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1379)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1379
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.332"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            4..198
                     /locus_tag="NBI57_002648"
     CDS             4..198
                     /locus_tag="NBI57_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HCD1823564.1"
                     /translation="MADESKFEQAKGNVKETVGNVTDNKNLENKGKEDKASGKAKEFV
                     ENAKEKATDFIDKVKGNKGE"
     gene            complement(432..1283)
                     /locus_tag="NBI57_002649"
     CDS             complement(432..1283)
                     /locus_tag="NBI57_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HCD1823565.1"
                     /translation="MQQFKWINILKGFAMGTSDLVPGVSGGTIALLLGIYNQFIASIS
                     GIFSRRFWPSFTFLIPIIIGMLLAMGSLSNLFNYLLSQHHIPTMFFFGGLIIGIVPYL
                     LKISNYKTSFTTKHYMMVIAGIAILIVITLMNNGDKHAGETLTLSTGLIIKYFIAGMC
                     ASSAMLLPGISGSFMLLVFGVYGTVMLAISEVVKLNFTGLPILLAVGFGVLAGFIISS
                     KIIQYFLTHHKLMTFALIIGFVVGSLFAVFPGLPTNIVMWFVSLVVFIIGFIVSLTLG
                     RITAENE"
BASE COUNT          560 a          214 c          215 g          390 t
ORIGIN      
        1 attatggcag acgaaagtaa atttgaacaa gcaaaaggta atgttaaaga aacagtaggt
       61 aatgttactg ataataaaaa tttagaaaac aaaggtaaag aagataaagc ttctggtaaa
      121 gcgaaagaat tcgttgaaaa tgcaaaagaa aaagcaactg attttattga taaagtaaaa
      181 ggtaacaaag gcgagtaatt ttactttttt attcctaata cactgcacat gccacttagt
      241 cggtataacc atcggctagg tggtttttgt attaaaaaag tggataccaa aatttattaa
      301 taattatttt aatgttagaa aaaaactaaa taaaaactcg ctaatgatat ccaataatat
      361 gtatacaaaa cgagacatat attgcatatg attaacgaga tactgaatat attttatcac
      421 ccctaaaatg attattcatt ttcagcggta attcgaccta aagtcaaact tacaataaaa
      481 ccgatgataa atactactaa tgaaacgaac cacatcacga tattagttgg taaacctgga
      541 aatactgcaa agagggagcc aacaacaaaa ccaatgatta atgcaaaagt cattagttta
      601 tgatgtgtta ggaaatactg gataattttg cttgaaataa tgaatccagc aagcacccca
      661 aatccgactg caagtagtat aggaagaccg gtaaagttaa gtttaacaac ttcagatatt
      721 gctagcatga ccgtaccata gacgccaaat actaataaca taaatgatcc tgaaatacct
      781 gggagtaaca tagcgctaga tgcacacata cctgcaataa agtatttaat aataagacca
      841 gttgatagag taagcgtttc tccagcatgt ttatcaccat tattcattaa tgtaataaca
      901 attaagatag cgataccagc tataaccatc atgtaatgtt tagttgtaaa tgacgtttta
      961 tagttagaaa ttttcaataa atatggaacg ataccaatga ttaatccacc aaagaaaaac
     1021 atagttggaa tatggtgttg gcttaataaa taattaaaaa gattacttag tgatcccatt
     1081 gccagtaaca ttccaattat aatggggatt aaaaatgtaa aacttggcca aaaacgtcgt
     1141 gagaatatgc cgctaattga agcgataaat tgattgtaaa tacctaacaa taatgcgata
     1201 gtcccaccgc taacaccagg taccaagtca ctcgttccca tagcaaaacc ttttagaata
     1261 ttaatccatt taaattgttg catgaataac tcctttcaaa cgattggaat aaaatcataa
     1321 atagcatcat accatattac aaatgtccta gtgaaatgat aacatatttt aaattcata
//
LOCUS       DAHHSC010000326         1373 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.333, whole genome shotgun sequence.
ACCESSION   DAHHSC010000326 DAHHSC010000000
VERSION     DAHHSC010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1373)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1373)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1373
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.333"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            1..1373
                     /gene="rsp"
                     /locus_tag="NBI57_002650"
                     /pseudo
     CDS             1..1373
                     /gene="rsp"
                     /locus_tag="NBI57_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501126.1"
                     /note="internal stop; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="AraC family transcriptional regulator Rsp"
BASE COUNT          501 a          223 c          179 g          470 t
ORIGIN      
        1 taccaaatat tgctttaagc aatccggtgt tcatagactg tgtaaactat attcatgaca
       61 atattgatgc gcacttgtct ttaaaagata tagcgatgca ttgcaacatc tctgaatcat
      121 attgttctaa tctttttgtt cgatatttga gtatgaattt taaagattat tttacaagta
      181 ttaaacttgt taacgccatc aatttattac tttctacaaa acattctatt acaactgttt
      241 ctgaattggc cggttttaac agtcatacaa attttgcaaa tcaatttaag aattacttgc
      301 attttagtcc taaacagttc cgttcactcg tttccaagat tactgaacca ccacaaatac
      361 acttccaaca agataacgta tcacaattca ctgagttaat ttcaacaatt gatttaacag
      421 ctcaactggc tacaaataca acagatattc atatagatga ttttaatccg aaagatcgaa
      481 gtcagcgtgc caaagtattt gtttgattta gtaatttcaa tgaactattt caatttattt
      541 tcaatgaata ttacgatatt aactttgagc acttaccaaa acctgtcgtt ttcattgacg
      601 atattcacga tattgagatt agccaaacga attacaacct gttaaataga tgttttgaaa
      661 agcttttcga aaaaaatata ggtttagcaa tcgctattaa atcaacacaa caattcgaaa
      721 cgatgaagca gttaatacta acatttttac aaggcaatca agactataag acaagcaaga
      781 aattagttaa atttatgctc gtattttgct ctaattctat gacagcagaa gaaattcatt
      841 tatgtcatct taaaattaaa aacaaaaata aagaaattaa atatagtgtg acagtcgatg
      901 gctttttaga aacttattca accgtggaac aagtttatga tgtcatgcag cgtcttaaat
      961 tccattatta ctttattgat attgaaaatt caaaaactgc aacacatctt atcactaaaa
     1021 accaacacta tcatcaaact gacactcatt ttgaacagta taagaaattt attttagatt
     1081 caggtatatc atcaacacaa tttgtatata ataacctgtc tgtaagcgga tttaaatata
     1141 ctaatgatgg taagaatcca attcaattat ctgacatagt gtatcactta atcgcattat
     1201 tacgatatgg cggtggcatt agctatcaat tattagatga ccattcaaat tatatttcct
     1261 tgtacaacaa atatggtagc cccctaccat taatgcattt atataaaatg tttagacctt
     1321 ttgttaatga agatattgaa attacaaata attatgtatt gagtcgtaaa gat
//
LOCUS       DAHHSC010000327         1368 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.334, whole genome shotgun sequence.
ACCESSION   DAHHSC010000327 DAHHSC010000000
VERSION     DAHHSC010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1368)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1368)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1368
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.334"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            complement(39..>1368)
                     /locus_tag="NBI57_002651"
     CDS             complement(39..>1368)
                     /locus_tag="NBI57_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCD1823566.1"
                     /translation="IITVIALILIMFMSAIESSIISLALPTIKQDLNAGNLISLIFTA
                     YFIALVIANPIVGELLSRFKIIYVAIAGLLLFSIGSFMCGLSTNFTMLIISRVIQGFG
                     SGVLMSLSQIVPKLAFEIPLRYKIMGIVGSVWGISSIIGPLLGGGILEFATWHWLFYI
                     NIPIAIIAIILVIWTFHFPEEETVAKSKFDTKGLTLFYVFIGLIMFALLNQQLLLLNF
                     LSFILAIVVAMCLFKVEKHVSSPFLPVVEFNRSITLVFITDLLTAICLMGFNLYISVY
                     LQEQLGLSPLQSGLVIFPLSVAWITLNFNLHRIEAKLSRKVIYLLSFTLLLVSSIIIS
                     FGIKLPVLIAFVLILAGLSFGYIYTKDSVIVQEETSPLQMKKMMSFYGLTKNLGASIG
                     STIMGYLYAIQSGIFGPNLHNVLSAVAVISIGLIVLWVVFFKEQSSQSKE"
BASE COUNT          535 a          237 c          173 g          423 t
ORIGIN      
        1 cttactttaa ttacactaca tcaattttaa tcataattct attcttttga ttgagatgac
       61 tgttctttaa aaaatacgac ccataaaaca ataagcccaa tgctaattac agcaacagca
      121 cttaacacat tgtgtaagtt tggaccaaag attcctgatt gtatcgcata aagatatccc
      181 attattgttg aacctattga tgccccaaga tttttagtta atccataaaa tgacatcatt
      241 ttcttcattt gtaatgggct agtttcctct tggacaatta cactatcttt cgtataaata
      301 tatccaaaac ttaatcctgc caaaattaac acaaatgcta taagtaccgg caatttaata
      361 ccaaatgaaa tgataatact acttactagt aacaatgtaa atgatagtaa gtaaataact
      421 ttccttgata gttttgcttc aattcgatgt aaattaaaat tcaatgtaat ccaagctaca
      481 gataaaggaa aaataaccaa tccactttgc aatggagata atcctagttg ttcttgaagg
      541 tagactgaaa tatataaatt gaatcccatt aaacaaatag ctgttagaag gtccgttata
      601 aaaactaaag taatcgaacg attaaattca accacaggta aaaatggtga agaaacatgt
      661 ttttctactt taaataaaca cattgccaca acgatagcta agataaaact caagaaattt
      721 aaaagtaata gctgttggtt taataaagca aacataatca atccaataaa tacataaaat
      781 agcgtgagcc ctttagtatc aaattttgat ttagcaactg tttcttcctc tggaaaatga
      841 aatgtccata taactaatat aatggcaatg atcgcaatcg gtatatttat gtagaataac
      901 caatgccatg ttgcaaattc taatatacca ccacctagaa gcggtccaat gatactcgaa
      961 ataccccaaa cactgccaac tatgcccata attttatatc gtaacggaat ttcaaatgct
     1021 aactttggaa caatttgcga taatgacatc aatacacctg atccaaaacc ttgtatcacg
     1081 cgtgatataa ttagcatagt aaaattagta cttaaaccac acataaaact acctatacta
     1141 aataaaagta atcctgcaat tgccacataa ataattttaa atctcgaaag taattctccc
     1201 actataggat tggcaattac taatgcgata aaatacgctg taaaaattaa tgaaattaaa
     1261 tttcctgcat ttaaatcttg tttaattgta ggtaatgcaa gcgaaataat agatgattca
     1321 atcgctgaca taaacataat taatattaaa gctataacag ttatgatt
//
LOCUS       DAHHSC010000328         1335 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.335, whole genome shotgun sequence.
ACCESSION   DAHHSC010000328 DAHHSC010000000
VERSION     DAHHSC010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1335)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1335)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1335
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.335"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            complement(120..1145)
                     /locus_tag="NBI57_002652"
     CDS             complement(120..1145)
                     /locus_tag="NBI57_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low specificity L-threonine aldolase"
                     /protein_id="HCD1823567.1"
                     /translation="MISFENDYLEGAHEKVLKRLVDTNLVQASGYGFDQFTAQAIEKI
                     KDTIDCPNATIRFLVGGTQTNQVVINSMLESYEGVISADTGHVAVHEGGAIEYSGHKV
                     ITIPSKEGKVSASDVETYMETFKSDFKKDHMVFPGMVYISHPTEYGTLYSKSELEELC
                     KVCKQYQLPLFMDGARLGYGLMSDQSDMTIKDIAKYCDVFYIGGTKIGALCGEAIVFT
                     KNNEPKQFTTRIKHHGALLAKGRLTGIQFLELFTDNLYFNISRHAIEMANKMKDGFKN
                     KGYRLYFDSPTNQQFFILSNEKIAELEQKVKFAVWEKYDDQHRVVRFATSWATTEENL
                     NKLLELI"
BASE COUNT          434 a          246 c          174 g          481 t
ORIGIN      
        1 taatgtaatt ttatatcatc aacggcttca aatgagtagt ttcatataaa tcaataaaca
       61 caactactca actgatctta attaataaat gatttgtcag atgacacata acagcattat
      121 tagattaatt ctaatagttt gtttaaattt tcttcggttg tcgcccagct ggttgcgaat
      181 ctaacaacac gatgttgatc atcgtatttt tcccaaacag caaatttaac tttttgttct
      241 aactctgcta ttttctcgtt acttaaaata aaaaattgtt gattggttgg agaatcaaag
      301 taaagacgat agcctttatt tttaaacccg tctttcatct tatttgccat ttcgatagca
      361 tgtctgctta tattaaaata taaattgtcc gtaaataatt ctaaaaattg tatgcctgtt
      421 aaccgtcctt ttgctaaaag ggcaccgtga tgtttgattc gagtggtaaa ttgtttcggt
      481 tcattatttt tcgtaaaaac aatggcttcc ccgcataatg cacctatctt cgtaccacct
      541 atataaaata catcacaata tttagcgatg tctttaatag tcatatctga ttgatcactc
      601 atcaatccat accctaatcg tgcaccatcc ataaataatg gaagctgata ttgcttacat
      661 actttgcata actcttccaa ttctgattta gagtataaag tgccatattc tgtaggatga
      721 gaaatatata ccattcctgg gaataccata tggtcctttt taaaatcact tttaaatgtc
      781 tccatgtaag tttcaacatc tgaagcacta acttttcctt ccttagaggg tatagtaatt
      841 actttatgtc cactatattc aattgcacca ccctcatgca cagcaacatg accagtgtct
      901 gctgaaatga ccccttcgta actttctaac attgaattaa taacaacctg attggtttgt
      961 gttccaccta ctaaaaaacg aattgtagca tttggacaat caattgtatc tttaatcttt
     1021 tcaattgcct gagctgtgaa ttgatcaaag ccatatcccg aagcttgtac aagatttgta
     1081 tctactaatc gttttaatac tttttcatga gcaccttcta aataatcatt ttcaaatgag
     1141 atcactacat ttccccctaa aactaatatc agcattttaa tatgataaac taatttcaaa
     1201 actagttcga tatttaaaat gtattgtgaa ttgttaaagt ttgtatcgca ttatcgcgaa
     1261 gttgaataaa tatattatac atcaaacaat acattaagaa aattaattaa tttttaatca
     1321 tagcgacttt tcatg
//
LOCUS       DAHHSC010000329         1314 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.336, whole genome shotgun sequence.
ACCESSION   DAHHSC010000329 DAHHSC010000000
VERSION     DAHHSC010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1314)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1314)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1314
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.336"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            complement(14..106)
                     /locus_tag="NBI57_002653"
     tRNA            complement(14..106)
                     /locus_tag="NBI57_002653"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(68..70),aa:Ser,seq:tga)
     gene            complement(145..220)
                     /locus_tag="NBI57_002654"
     tRNA            complement(145..220)
                     /locus_tag="NBI57_002654"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(185..187),aa:Asp,seq:gtc)
     gene            complement(231..320)
                     /locus_tag="NBI57_002655"
     tRNA            complement(231..320)
                     /locus_tag="NBI57_002655"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(282..284),aa:Ser,seq:tga)
     gene            complement(330..403)
                     /locus_tag="NBI57_002656"
     tRNA            complement(330..403)
                     /locus_tag="NBI57_002656"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(367..369),aa:Ile,seq:cat)
     gene            complement(432..505)
                     /locus_tag="NBI57_002657"
     tRNA            complement(432..505)
                     /locus_tag="NBI57_002657"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(469..471),aa:Met,seq:cat)
     gene            complement(527..602)
                     /locus_tag="NBI57_002658"
     tRNA            complement(527..602)
                     /locus_tag="NBI57_002658"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(567..569),aa:Ala,seq:tgc)
     gene            complement(620..693)
                     /locus_tag="NBI57_002659"
     tRNA            complement(620..693)
                     /locus_tag="NBI57_002659"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(657..659),aa:Pro,seq:tgg)
     gene            complement(703..776)
                     /locus_tag="NBI57_002660"
     tRNA            complement(703..776)
                     /locus_tag="NBI57_002660"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(740..742),aa:Arg,seq:acg)
     gene            complement(795..883)
                     /locus_tag="NBI57_002661"
     tRNA            complement(795..883)
                     /locus_tag="NBI57_002661"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(847..849),aa:Leu,seq:taa)
     gene            complement(894..968)
                     /locus_tag="NBI57_002662"
     tRNA            complement(894..968)
                     /locus_tag="NBI57_002662"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(934..936),aa:Gly,seq:gcc)
     gene            complement(972..1053)
                     /locus_tag="NBI57_002663"
     tRNA            complement(972..1053)
                     /locus_tag="NBI57_002663"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1017..1019),aa:Leu,seq:tag)
     gene            complement(1057..1132)
                     /locus_tag="NBI57_002664"
     tRNA            complement(1057..1132)
                     /locus_tag="NBI57_002664"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1097..1099),aa:Lys,seq:ttt)
     gene            complement(1138..1213)
                     /locus_tag="NBI57_002665"
     tRNA            complement(1138..1213)
                     /locus_tag="NBI57_002665"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1178..1180),aa:Thr,seq:tgt)
     gene            complement(1230..1305)
                     /locus_tag="NBI57_002666"
     tRNA            complement(1230..1305)
                     /locus_tag="NBI57_002666"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1270..1272),aa:Val,seq:tac)
BASE COUNT          352 a          354 c          313 g          295 t
ORIGIN      
        1 taataaaaaa taatggcgga ggaagaggga ttcgaacccc cgcggcccgt taaggccctg
       61 tcggttttca agaccgatcc cttcagccgg acttgggtat tcctccatta ttataggtaa
      121 atcgctatta attataaaat taaatggcgg tctcgacggg aatcgaaccc gcgatctcct
      181 gcgtgacagg caggcgtgtt aaccgctaca ctacgagacc attagtaaaa cggaggaaga
      241 gggattcgaa cccccgcgag ccgttaagcc cctgtcggtt ttcaagaccg atcccttcag
      301 ccggacttgg gtattcctcc aaaattatat ggaccttgca ggactcgaac ctgcgaccga
      361 acggttatga gccgttagct ctaaccaact gagctaaagg tcctaaatat aattttacaa
      421 ctaataaata gtggcggtgg aggggatcga acccccgacc tcacgggtat gaaccgtacg
      481 ctctagccag ctgagctaca ccgccttata tagtttgtaa ataatatggt ggagactagc
      541 gggatcgaac cgctgacctc ctgcgtgcaa agcaggcgct ctcccagctg agctaagccc
      601 ccataataat tacagtatat cgggaagaca ggattcgaac ctgcgacccc ttggtcccaa
      661 accaagtgct ctaccaagct gagctacttc ccgtataatt aacgcgcccg ataggagtcg
      721 aacccataac ctcttgatcc gtagtcaaac gctctatcca attgagctac gggcgcatat
      781 gtttttattg aaaatggtgc cgaggaccgg aatcgaaccg gtacggtgat cactcaccgc
      841 aggattttaa gtcctgtgcg tctgccagtt ccgccacccc ggcactataa aaatggagca
      901 gaagacggga ttcgaacccg cgaccccaac cttggcaagg ttgtattcta ccgctgaact
      961 acttctgcat atgcgggtga agggagtcga acccccacgc cgtaaggcgc tagatcctaa
     1021 gtctagtgcg tctgccaatt ccgccacacc cgcaaatggt gagccataga ggattcgaac
     1081 ctctgaccct ctgattaaaa gtcagatgct ctaccaactg agctaatggc tcttccatgg
     1141 tgccggccag aggacttgaa cccccaacct actgattaca agtcagttgc tctaccaatt
     1201 gagctaggcc ggcaatatgt aagaataaat ggtggagaat gacgggttcg aaccgccgac
     1261 cctctgcttg taaggcagat gctctcccag ctgagctaat tctccgattt aaaa
//
LOCUS       DAHHSC010000330         1312 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.337, whole genome shotgun sequence.
ACCESSION   DAHHSC010000330 DAHHSC010000000
VERSION     DAHHSC010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1312)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1312)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1312
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.337"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            <1..718
                     /locus_tag="NBI57_002667"
     CDS             <1..718
                     /locus_tag="NBI57_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HCD1823568.1"
                     /translation="LYNNYFVAMMVYYVVISLIMLDNFKKMKNREYQKEIAEKNRHIN
                     TLIAEQERHRIGQDLHDTLGHVFASLSLKSELAYKLIDTDVEKVKAELLAINKLSRES
                     LNKVREIIDDVKLPSFIEEIDSIRKVLKDADIDFTFENKELAQVLSPTKQSMLVMITR
                     EAINNVIKHANASKVHGKLKTVNNHKLLLMIEDDGKGIDSDCEVKSISQRVQHLNGTL
                     AVDSTNGTKIIIEISTGGIA"
     gene            715..>1312
                     /locus_tag="NBI57_002668"
     CDS             715..>1312
                     /locus_tag="NBI57_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCD1823569.1"
                     /translation="MTSLIIAEDQNMLRQAMVQLIKLHGDFEILADTDNGLDAMKLIE
                     EYNPNVVILDIEMPGMTGLEVLAEIRKKHLNIKVIIVTTFKRPGYFEKAVVNDVDAYV
                     LKERSIEELVETINKVNNGEKEYSATLMTSFFVDKNPLTPKEQIVLREIGNGLSSKEI
                     SEKLFLTDGTVRNYTSVIIDKLFADNRFDAWKKANEKGW"
BASE COUNT          529 a          141 c          238 g          404 t
ORIGIN      
        1 attgtataac aactattttg ttgcaatgat ggtttattat gtcgttatat cgttaataat
       61 gctagataat tttaaaaaaa tgaaaaaccg tgaatatcaa aaagaaatag cagaaaaaaa
      121 tagacatatt aatacattaa ttgctgaaca agagcgacat agaattggtc aagacttaca
      181 tgatacgtta gggcatgtgt ttgcaagttt atcattaaaa tcagaattag cttataaact
      241 aatagatact gatgtagaaa aagtaaaagc tgaattatta gcaattaata aattatctcg
      301 tgaatcattg aacaaagttc gagaaattat tgatgatgta aaattaccat catttattga
      361 agagattgat agtatacgta aagttttaaa agatgctgat attgatttta catttgaaaa
      421 taaagaatta gcgcaagtat taagtcctac taaacaatct atgttagtta tgattacgcg
      481 tgaagcgata aataatgtta ttaaacatgc aaatgcttca aaagttcatg gtaaattaaa
      541 aactgtaaac aatcataaat tactgcttat gattgaagat gatggcaaag gtatcgatag
      601 tgattgtgag gtgaaaagta tttcacagcg tgtacaacat ttaaatggaa ctttagcagt
      661 cgactcaaca aatggaacta aaataatcat tgaaatctca acaggaggaa tagcatgaca
      721 tctttaatta ttgcagaaga tcaaaatatg ttacgacagg caatggttca attaattaaa
      781 ctacatggtg attttgaaat tttagcagat actgataatg gtctcgatgc aatgaaactt
      841 attgaagaat ataatcctaa cgttgttatt ttagatatag aaatgccagg catgactgga
      901 cttgaagttt tagcggaaat tagaaaaaag catttgaata ttaaagtgat tattgtaaca
      961 acttttaaaa gaccgggata ctttgaaaaa gcagttgtga atgatgtgga tgcatatgtt
     1021 ttaaaagaac gttctataga agaattggtg gaaaccatta ataaagtaaa taacggagag
     1081 aaagaatata gcgccacatt gatgacttca ttttttgtag ataaaaaccc attaacgccc
     1141 aaagaacaaa ttgtattaag ggaaattggc aatggtttaa gtagtaaaga aataagtgaa
     1201 aaattatttt tgacagatgg aacagttaga aattatacat ctgttataat tgataaatta
     1261 tttgcagata atcgttttga tgcttggaaa aaggcaaatg aaaaaggctg ga
//
LOCUS       DAHHSC010000331         1301 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.338, whole genome shotgun sequence.
ACCESSION   DAHHSC010000331 DAHHSC010000000
VERSION     DAHHSC010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1301)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1301)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1301
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.338"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            complement(184..1275)
                     /locus_tag="NBI57_002669"
     CDS             complement(184..1275)
                     /locus_tag="NBI57_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-deoxy-7-phosphoheptulonate
                     synthase/chorismate mutase"
                     /protein_id="HCD1823570.1"
                     /translation="MSNKLESYRSEIVSLNHQILDLLSKRGELAQKIGEEKLKQGTRI
                     YDPQREKEMLNDLIDSNKGPFNDNTIKQLFKEIFKASTDLQKSENEKHLYVSRKLKPE
                     DTIVTFDNGGIIGDGNKSFVFGPCSVESFEQVEAVAKNLHAKGEKFIRGGAFKPRTSP
                     YDFQGLGVEGLKILKQIKDKYDLNVVSEIVNPNDFEVADEYLDVFQIGARNMQNFELL
                     KEAGRTKKPILLKRGLSATIEEFVYAAEYIASQGNQNIILCERGIRTYEKATRNTLDI
                     SAVPILKQGTHLPVMVDVTHSTGRKDIMLPTAKAALAVGADGVMAEVHPDPSVALSDA
                     GQQMDLDEFQAFYDELKPLADLYNAKKLK"
BASE COUNT          393 a          241 c          190 g          477 t
ORIGIN      
        1 aagtgtcgaa gtattataac aatcgttaag tgcagtatac ctttatttct atacaaaaaa
       61 taatagccac tctaaaagta atatactctt agaatggctg taagtttaaa attgtcgatt
      121 caaagataat attctacttc aacatgacat attagttaag tagtctatag tttccttgga
      181 atattatttt aactttttag cgttatataa atcagctaaa ggctttaatt catcataaaa
      241 tgcttggaat tcatctaaat ccatttgttg acccgcatca ctaagtgcaa cagatggatc
      301 tggatgcacc tcagccataa ctccatcagc accaactgct aatgctgctt tcgcagttgg
      361 taacatgata tctttacgac ctgtactatg cgtaacatct accatgactg gtaagtgtgt
      421 accttgtttt aaaattggta ctgctgaaat atctaaagtg ttacgtgtcg ccttttcata
      481 agttcggatt ccacgttcac ataaaataat gttttgatta ccttgtgaag caatgtattc
      541 agctgcataa acaaactctt cgattgtagc agataaacca cgttttaata gaataggctt
      601 tttcgtacgg ccagcttctt ttaataactc gaagttttgc atattacgtg caccaatttg
      661 gaatacgtct aaatactcat cagccacttc aaaatcattt ggatttacga tttcgctgac
      721 aacatttaaa tcatatttat ctttaatctg tttaagtatt ttaagtcctt caacacctag
      781 gccttggaaa tcatatggtg atgtacgtgg tttaaatgca ccgccacgaa taaatttttc
      841 acctttagca tgtaagtttt tagcaacagc ttcaacttgt tcaaatgatt caactgaaca
      901 tggcccaaat acaaatgatt tattgccgtc tccaataatg cccccattat caaatgttac
      961 aatcgtatct tcaggtttca acttacgtga tacgtataag tgtttttcat tttcagattt
     1021 ttgtaaatct gtagaggctt taaaaatttc tttaaataat tgcttaattg tattatcgtt
     1081 aaatggtcct ttgttgctat caattaagtc gttaagcatt tctttttcgc gttgtggatc
     1141 atagatacgt gtaccttgtt ttaatttttc ctccccaatt ttttgtgcta gttcaccacg
     1201 tttagataat aaatctaaaa tttgatgatt cagtgagaca atctcacttc tgtatgattc
     1261 taatttatta ctcatcctta ctcacctcta cgaattattt c
//
LOCUS       DAHHSC010000332         1281 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.339, whole genome shotgun sequence.
ACCESSION   DAHHSC010000332 DAHHSC010000000
VERSION     DAHHSC010000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1281)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1281)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1281
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.339"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            100..720
                     /locus_tag="NBI57_002670"
     CDS             100..720
                     /locus_tag="NBI57_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000673114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1823571.1"
                     /translation="MKFTEVEVIEHLVKAYKEAGKPTYPHENLYRGRNHSISGIGEDL
                     LGAYLISRLEGVQIFIDQPLSMIDKSLSTRYPDLLICEDNEIKNILEVKMDLGYQRKD
                     FIDYCRKKEEWISNIVGKQCVLSRKREDKIPMNIADDIKFHVVIYSENNGPKRFDEEI
                     MPIVNETCPHIEVYVLTSGQHPNLVNVNLEGININKDEFEILVNAL"
BASE COUNT          450 a          151 c          226 g          454 t
ORIGIN      
        1 ctcttaattt attaggaggt tttttgtatt tatgctttca aatgtgtgat atactttgtt
       61 tgttgaaata tagagtatct atagataggg tgattgagta tgaaattcac tgaagtggaa
      121 gttatcgaac atcttgtaaa ggcatataaa gaagcaggaa agcctactta tcctcatgaa
      181 aatttatatc gaggacgtaa tcatagtatt tcaggtattg gagaagactt gctgggtgct
      241 tatttgatta gtagattgga aggtgtccaa atatttattg atcagccttt atctatgatt
      301 gataaatctt taagtacaag atatccggat ttattaattt gtgaagataa tgaaattaaa
      361 aatatactag aagttaaaat ggacttagga tatcaaagaa aagattttat agattattgc
      421 cgaaagaaag aagaatggat ttcaaatatc gtaggaaaac agtgtgtatt gtctagaaag
      481 agagaagaca aaattcctat gaatatagct gatgatatta aatttcatgt tgtgatttac
      541 agtgaaaaca atggaccgaa gcggtttgat gaagaaatca tgcctatcgt taatgaaaca
      601 tgtccacata ttgaagtata tgtcctaaca agcggtcaac accctaattt agtaaatgtt
      661 aatcttgaag gtattaatat taataaagat gaatttgaaa tattagtaaa tgcgttataa
      721 aaaaatagag catcctccac gttatggagg tgctctgttt tttattgaaa agtatcaagt
      781 taattaattt aatatgctta ataagttcta ccttgacctt tttctctagc ttctgttcga
      841 tctcttatgt actcagtaca tactggattt tctgttaatt catcaactgt attagtttaa
      901 tcagaagacg tgtctacttt gtaagcttct aagaacttat tatatgatat agcgtttgag
      961 ttttgttgtt cttctataaa tttctgtagt tatttttcaa aaaccgcatc attaactgat
     1021 aagcagaggc gtatcataag taaaactaaa aaattctgta tgaggagata ataatttgga
     1081 gggtgttaaa tggtggacat taaatccacg ttcattcaat atataagata tatcacgata
     1141 attgcgcata taacttaagt agtagctaac agttgaaatt aggccctatc aaattggttt
     1201 atatctaaaa tgattaatat agaatgcttc tttttgtcct tattaaatta taaaagtaac
     1261 tttgcaatag aaacagttat t
//
LOCUS       DAHHSC010000333         1270 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.340, whole genome shotgun sequence.
ACCESSION   DAHHSC010000333 DAHHSC010000000
VERSION     DAHHSC010000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1270)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1270)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1270
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.340"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            <1..1112
                     /locus_tag="NBI57_002671"
     CDS             <1..1112
                     /locus_tag="NBI57_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001628779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="coagulase"
                     /protein_id="HCD1823572.1"
                     /translation="DTLKKNKQEFLKDIEEIQLKNSDLKDFNNTEQHNADVEINNLEN
                     KVLMVGYTFYNTNKDEVEELYSELDLIVGEVQDKSDKKRAVNQRMLNRKKEDLEFIID
                     KFFKKIQQERPESIPALTSEKNHNQTMALKLKADTEAAKNDVSKRSKRSLNTQNNKST
                     TQEISEEQKAEYQRKSEALKERFINRQKSKNESVVSLIDDEDDNENDRQLVVSAPSKK
                     PTTPTTYTETTTQVPMPTVERQTQQQIVYKTPKPLAGLNGESHDFTTTHQSPTTSNHT
                     HNNVVEFEETSALPGRKSGSLVGISQIDSSHLTEREKRVIKREHVREAQKLVDNYKDT
                     HSYKDRLNAQQKVNTLSEGHQKRFNKQINKVYNGK"
BASE COUNT          562 a          181 c          204 g          323 t
ORIGIN      
        1 ttgatacatt aaagaaaaat aaacaagagt ttttaaaaga tattgaagaa atacaactga
       61 aaaatagtga tttaaaggac tttaacaata cagagcaaca taatgccgac gtagaaataa
      121 acaatttaga aaataaagta ttaatggtag ggtatacatt ctataataca aataaggacg
      181 aagttgaaga attatatagt gagttagatt tgattgttgg agaagttcaa gataagtcgg
      241 ataaaaaaag agcagtaaat caaaggatgt taaatagaaa aaaagaggat ttagaattta
      301 ttatagataa attttttaaa aaaattcaac aagaacgtcc agagagtata ccagcattaa
      361 ctagtgaaaa aaatcataat cagactatgg cattaaagtt aaaagcagat acagaagctg
      421 ctaaaaatga cgtatcaaaa agaagtaaaa gaagtttaaa tactcaaaat aataaatcta
      481 caacacaaga aatttctgaa gaacaaaaag ctgaatatca aagaaagtca gaggcattaa
      541 aagaaagatt tataaacaga caaaaatcta aaaatgagtc tgtggtttca ctaatcgatg
      601 acgaagacga caacgaaaac gacaggcaac ttgtggtttc tgcgccatca aagaaaccaa
      661 caacaccgac tacatatact gaaacaacga ctcaggtacc aatgcctaca gttgagcgtc
      721 aaactcagca acaaatcgtt tacaaaacac caaaaccatt agctggatta aatggtgaaa
      781 gtcatgattt cacaacaacg catcaatcac caacaacttc aaatcatacg cataataatg
      841 ttgttgaatt tgaagaaacg tctgctttac ctggtagaaa atcaggatca ctggttggta
      901 taagtcaaat tgattcttct catctaactg aacgtgagaa gcgtgtaatc aagcgtgaac
      961 acgttagaga agctcaaaag ttagttgata attataaaga tacacatagt tataaagacc
     1021 gattaaatgc acaacaaaaa gtaaatactt taagtgaagg tcatcaaaaa cgttttaata
     1081 aacaaatcaa taaagtatac aatggcaaat aatattaatg catggctgca aagcaaataa
     1141 tgagtttgtc gtaaaaataa caacatttta aactagcaat aaataatatc aaagtcatca
     1201 tttcaatgat gcaatctagt atagtccaca ttctaaacag gtgtggacta ttactttttt
     1261 cactttatat
//
LOCUS       DAHHSC010000334         1264 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.341, whole genome shotgun sequence.
ACCESSION   DAHHSC010000334 DAHHSC010000000
VERSION     DAHHSC010000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1264)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1264)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1264
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.341"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            115..888
                     /gene="lpl1"
                     /locus_tag="NBI57_002672"
     CDS             115..888
                     /gene="lpl1"
                     /locus_tag="NBI57_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_047335196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein Lpl1"
                     /protein_id="HCD1823573.1"
                     /translation="MRYLKRLSWYISILILIVVIAGCGKGNETKEGSKEEQIKKSFAK
                     TLDMYPIKNLEDLYDKEGYRDSEFKKSDKGTWTIYTDFAKSNKPGELDDEGMVLNLDR
                     NTRTAKGHYFVTTFYRNGKLPDEKNYKIEMKNNKIILLDEVKDDKLKQKIENFKFFGQ
                     YANLKELRKYNNGDVSINENVPSYDVEYKMSNKDEIVKELRSRYNISTEKSPILKMHI
                     DGDLKGSSVGYRKLEIDFSKRENSKLSVIEFLSYKPAKK"
     gene            919..>1264
                     /locus_tag="NBI57_002673"
     CDS             919..>1264
                     /locus_tag="NBI57_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001269759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein"
                     /protein_id="HCD1823574.1"
                     /translation="MGYLKKLALFISVIILGIFIIGCDSSSDTAEKAKEDSKEEQIKK
                     SFAKTLDMYPIKNLEDLYDKEGYRDGEFKKGDKGTWTLLTSFSKSNKPGEIDDEGMVL
                     YLNRNTKKATGYY"
BASE COUNT          539 a          125 c          244 g          356 t
ORIGIN      
        1 taaattgatt gtatatgtat attttggtaa cgtaaaagag aaatatacaa aataattaat
       61 tatttatatg aaaagagaat ataaatgaag tataaaacaa agagacgtga aacgatgcga
      121 tatttaaaaa gactttcatg gtacataagc atcttaattt taatagttgt tatagctggt
      181 tgtggcaaag gtaatgaaac aaaagaaggt tcaaaagaag aacaaatcaa aaagagcttt
      241 gcgaaaacgt tagatatgta tccaatcaaa aatctcgagg atttatacga caaagaagga
      301 tatcgagata gcgaatttaa aaaaagtgat aaagggacat ggacgatata cacagatttc
      361 gctaaaagta ataaaccggg tgaattggat gatgaaggta tggttttaaa tctggatagg
      421 aatactagaa ccgctaaagg ccattatttt gttactacat tttaccggaa tggtaaacta
      481 ccagatgaaa agaattataa aattgaaatg aaaaataata agattatttt attagatgaa
      541 gtaaaagatg ataagctcaa gcagaaaata gaaaacttta aatttttcgg tcaatatgca
      601 aatcttaaag aattgagaaa atataataat ggtgatgttt caattaatga aaatgttcct
      661 agttatgatg ttgaatacaa aatgagcaat aaagatgaaa tagttaagga attaagaagt
      721 cgttataata tttcgactga aaaatcacct atattaaaaa tgcatattga tggggacctg
      781 aaaggcagtt ctgtaggtta tagaaagtta gaaattgact tttcaaaacg tgaaaacagc
      841 aaattatcag tcattgaatt tttaagttat aaaccagcga aaaaatagta ttgataagga
      901 atattaggat gtgaaaaaat gggatatcta aaaaagcttg cactgttcat aagtgttatt
      961 attttgggca tttttataat aggttgtgat agttcaagcg atactgcgga aaaagcaaaa
     1021 gaagattcaa aagaagaaca aatcaaaaag agctttgcga aaacgttaga tatgtatcca
     1081 attaagaatc tcgaggattt atacgacaaa gaaggatata gagatggtga atttaaaaag
     1141 ggcgataagg ggacttggac tttactcaca agtttttcaa aaagtaacaa accgggtgaa
     1201 atagatgacg aaggcatggt tttatatcta aatagaaata ccaaaaaggc aacaggttat
     1261 tatt
//
LOCUS       DAHHSC010000335         1260 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.342, whole genome shotgun sequence.
ACCESSION   DAHHSC010000335 DAHHSC010000000
VERSION     DAHHSC010000335.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1260)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1260)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1260
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.342"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            112..1155
                     /gene="recA"
                     /locus_tag="NBI57_002674"
     CDS             112..1155
                     /gene="recA"
                     /locus_tag="NBI57_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HCD1823575.1"
                     /translation="MDNDRQKALDTVIKNMEKSFGKGAVMKLGDNIGRRVSTTSTGSV
                     TLDNALGVGGYPKGRIIEIYGPESSGKTTVALHAIAEVQSNGGVAAFIDAEHALDPEY
                     AQALGVDIDNLYLSQPDHGEQGLEIAEAFVRSGAVDIVVVDSVAALTPKAEIEGEMGD
                     THVGLQARLMSQALRKLSGAISKSNTTAIFINQIREKVGVMFGNPETTPGGRALKFYS
                     SVRLEVRRAEQLKQGQEIVGNRTKIKVVKNKVAPPFRVAEVDIMYGQGISKEGELIDL
                     GVENDIVDKSGAWYSYNGERMGQGKENVKMYLKENPQIKEEIDRKLREKLGISDGDVE
                     ETEDAPKSLFDEE"
BASE COUNT          447 a          175 c          267 g          371 t
ORIGIN      
        1 caagatttta tacgaacaaa tattcgcaaa acacttgtat tttattttga atccttgtat
       61 agtattgata agataattaa aagatagcaa tttcaattag gaggtctcgc tttggataac
      121 gatcgtcaaa aagctttaga tacagtaatt aaaaatatgg agaaatcttt cggtaaaggt
      181 gccgtaatga agttgggtga caatataggt cgccgagttt caactacatc aactggttca
      241 gttacattag ataatgcgct aggtgtaggt ggctatccta aaggacgaat tattgaaatt
      301 tatggtcctg aaagttctgg taagacaaca gtagcgcttc acgctattgc tgaagtacaa
      361 agtaatggcg gggtggcagc atttatcgat gctgaacatg ctttagatcc agaatatgct
      421 caagcattag gcgtagatat cgataattta tatttatcgc aaccggatca tggtgaacaa
      481 ggtcttgaaa tcgccgaagc atttgttaga agtggtgcag ttgatattgt agttgtagac
      541 tcagttgctg ctttaacacc taaagctgaa attgaaggag aaatgggaga cactcacgtt
      601 ggtttacaag ctcgtttaat gtcacaagcg ttacgtaaac tttcaggtgc tatttctaaa
      661 tcaaatacaa ctgctatttt catcaaccaa attcgtgaaa aagttggtgt tatgttcggt
      721 aatccagaga ctacaccagg tggacgtgca ttaaaattct atagttcagt aagactagaa
      781 gtacgtcgtg cagaacagct taaacaagga caagaaattg taggtaatag aactaaaatt
      841 aaagtcgtta aaaataaagt ggcaccacca tttagagtag ctgaagttga tattatgtat
      901 ggacaaggta tttctaaaga gggtgaactt attgatttag gtgttgaaaa cgacatcgtt
      961 gataaatcag gagcatggta ttcttacaat ggcgaacgaa tgggtcaagg taaggaaaat
     1021 gttaaaatgt acttgaaaga aaatccacaa attaaagaag aaattgatcg taaattgaga
     1081 gaaaaattag gtatatctga tggtgatgtt gaagaaacag aagatgcacc aaagtcatta
     1141 tttgacgaag aatagtacac aaatttatat ctatagttaa acttagcaaa tatccttata
     1201 ggattgattg aaagtgatat tcatctcata aagctagaat aatatctaac tttatgggat
//
LOCUS       DAHHSC010000336         1257 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.343, whole genome shotgun sequence.
ACCESSION   DAHHSC010000336 DAHHSC010000000
VERSION     DAHHSC010000336.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1257)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1257)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1257
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.343"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            101..502
                     /locus_tag="NBI57_002675"
     CDS             101..502
                     /locus_tag="NBI57_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000739557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formyl peptide receptor-like 1 inhibitory
                     protein"
                     /protein_id="HCD1823576.1"
                     /translation="MKKNITKTIIASTVIAAGLLTQTNDAKAFFSYEWKGLEIAKNLA
                     DQAKKDDERIDKLMKESDKNLTPYKAETVNDLYLIVKKLSQGDVKKAVVRIKDGGPRD
                     YYTFDLTRPLEENRKNIKVVKNGEIDSIYWD"
     gene            462..554
                     /locus_tag="NBI57_002676"
                     /pseudo
     CDS             462..554
                     /locus_tag="NBI57_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499655.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(871..1200)
                     /gene="ecb"
                     /locus_tag="NBI57_002677"
     CDS             complement(871..1200)
                     /gene="ecb"
                     /locus_tag="NBI57_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="complement convertase inhibitor Ecb"
                     /protein_id="HCD1823577.1"
                     /translation="MKKNFIGKSILSIAAISLTVSTFAGESHAQTKNVEAAKKYDQYQ
                     TNFKKQVNKKVVDAQKAVNLFKRTRTVATHRKAQRAVNLIHFQHSYEKKKLQRQIDLV
                     LKYNTLK"
BASE COUNT          437 a          187 c          183 g          450 t
ORIGIN      
        1 aattaatgaa gtgttaactg gtaaatgcat gaaaccgttt gtaaaatagc agtgtactcg
       61 cattagtaag tacaaattta ttttacaggg agaacttatt atgaaaaaaa atatcacaaa
      121 aactattatc gcatctacag taatcgctgc aggtctttta actcaaacta atgatgctaa
      181 agctttcttt agttatgaat ggaaaggttt agaaatcgca aaaaatttag cagatcaagc
      241 taaaaaggac gatgaacgta ttgataagtt aatgaaagag tctgataaaa atctaactcc
      301 ttacaaagct gaaactgtta atgatctgta ccttattgtt aaaaaattaa gccaaggtga
      361 tgtgaagaaa gcagttgtca gaattaaaga tggtggtcct agagattact atacttttga
      421 cttaactcgt cctttagaag agaacagaaa aaatattaaa gttgttaaaa acggtgaaat
      481 cgactcgatt tattgggatt aattaacata ctgaattaaa tagttgtacg ccaaacgtta
      541 gaaaacaatg ctaacgtatt ggcgtgcttt tttattttaa gtaacttcca atttatttag
      601 catctttacg actgtttaat aaagcacgta tgattaacac ggttgccact atatccgtta
      661 caatttttat gattgttaat acattcgatt tccttttcac aatagccacc tcgcttgttc
      721 aaaacataaa caactattgc attcactttt aagtaattat ttatatgatt tatcccaata
      781 agctcaccat tcaaataaac acaatattat aaaataatta tacctttgga tatagcaaaa
      841 agccacactc tatagcatgg cttctatcaa ttattttaaa gtattatatt ttaaaactag
      901 atcgatttgt ctttgtaatt ttttcttttc atagctgtgt tggaaatgaa ttaaattaac
      961 agctctttgt gctttacggt gtgttgcaac agttcttgta cgtttgaaca agtttacagc
     1021 tttttgtgca tccacaactt ttttatttac ttgtttttta aagtttgttt gatactgatc
     1081 atatttttta gcagcttcaa cgtttttagt ttgtgcatga gattcaccgg caaacgttga
     1141 taccgttaaa ctaatagcag ctatgcttaa aattgatttc ccaataaaat tctttttcat
     1201 aattaataac acctatcctc aaatatttat tttgtatata tttataataa tagacag
//
LOCUS       DAHHSC010000337         1247 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.344, whole genome shotgun sequence.
ACCESSION   DAHHSC010000337 DAHHSC010000000
VERSION     DAHHSC010000337.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1247)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1247)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1247
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.344"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            <1..865
                     /locus_tag="NBI57_002678"
     CDS             <1..865
                     /locus_tag="NBI57_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HCD1823578.1"
                     /translation="HNIMLNVKLGLNITCLFEKPSVFKSLVSQIKRLKFDSLIIDNAN
                     LSSPYLMGESDELLLKNILHFKNLKQVINELDIEQEKLIFLNVENHKLLNNKERDLSN
                     SAPLIYKTLSALYHNFDGFGLNIFDNHHTFNAMHLYDKNGFKTTLGLILEKFIEYVSK
                     PKYENSYYSIFDIENYYCLVIYDWRVIESETIMSNFEDSQVYINFKNNVLNDKYLIVI
                     ETLDENSGNINHLISKELRDKYEWNPSLLSKIDNYLKPAIEIKEHNFSDNSLNINVTF
                     NALYIIKIGKK"
     gene            1010..>1247
                     /gene="sarX"
                     /locus_tag="NBI57_002679"
     CDS             1010..>1247
                     /gene="sarX"
                     /locus_tag="NBI57_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001090984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator SarX"
                     /protein_id="HCD1823579.1"
                     /translation="MNTEKLETLLGFYKQYKALSEYIDKKYKLSLNDLAVLDLTMKHC
                     KDEKVLMQSFLKTAMDELDLSRTKLLVSIRRLIEK"
BASE COUNT          502 a          135 c          193 g          417 t
ORIGIN      
        1 acataacatc atgttgaatg tgaaattagg attgaacatt acctgtttat ttgaaaaacc
       61 ttcagttttt aaatcactag tatcacaaat aaagcgactt aaattcgatt cgttaataat
      121 agataatgca aatttaagta gcccttattt gatgggggaa agtgatgagt tactattgaa
      181 aaatattttg cattttaaaa atttaaaaca agtaattaat gaattggata ttgaacaaga
      241 aaagcttatt tttctaaatg ttgaaaatca taaactgctt aataataaag aacgagattt
      301 aagtaatagt gctccattaa tttataagac attaagtgcg ctgtatcaca actttgatgg
      361 ctttggatta aacatttttg ataatcatca tacatttaat gcgatgcatc tatatgataa
      421 aaatggattt aaaacaacac taggtcttat attggaaaaa tttatcgaat atgtctcgaa
      481 accaaaatac gaaaacagtt attattctat ttttgatata gagaattatt attgcctggt
      541 tatttatgat tggcgagtga ttgagagcga gacaattatg agcaattttg aggatagtca
      601 agtttatata aatttcaaaa ataatgtttt aaacgataaa tatctaattg taatagaaac
      661 attggacgaa aatagtggca acattaatca tttgatttct aaagaattaa gagataaata
      721 tgaatggaac cctagtttac tatctaaaat tgacaactac cttaaaccag caatcgagat
      781 taaagagcat aattttagtg ataattcctt gaatattaac gttactttta atgcgttata
      841 cataattaaa ataggaaaaa aataacacct tgatatgtat tgcaaaattt aatttgcatt
      901 tgttggtaat tttgacaacg tattaaaaac aaatgagaaa gacaatggca aaaaatttaa
      961 gataataatg acagaagcat tgtctttata tatttggggg tgcaacattt tgaatactga
     1021 gaaattagaa acattgcttg gcttctataa acaatataaa gcattatctg aatatattga
     1081 taaaaaatat aagttgtcgc taaatgattt agcagtctta gatttaacga tgaagcattg
     1141 caaagatgaa aaagtactta tgcaatcatt tttaaaaact gcaatggatg agctagattt
     1201 aagtaggaca aaattattag tttctataag aagactaatt gaaaaag
//
LOCUS       DAHHSC010000338         1247 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.345, whole genome shotgun sequence.
ACCESSION   DAHHSC010000338 DAHHSC010000000
VERSION     DAHHSC010000338.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1247)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1247)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1247
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.345"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            <1..536
                     /locus_tag="NBI57_002680"
     CDS             <1..536
                     /locus_tag="NBI57_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016170789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4888 domain-containing protein"
                     /protein_id="HCD1823580.1"
                     /translation="LTTLGTGLHSSYLGLNTNKVVKTAKAEEKMTNGQLWKKVKDSLI
                     DSNIISGNENEEITVTYVNKTGYSSSVSAYGNNNDDFSSTPSNFSKLKEIDLKKDNVP
                     SDDFNTTVSGEDSWKTLTSKLKEKGLVTDGQTVTIHCNDKSDNTKSSVSGKVGADLTS
                     GNGTTFKKRFIDKITID"
     gene            complement(1009..>1247)
                     /locus_tag="NBI57_002681"
     CDS             complement(1009..>1247)
                     /locus_tag="NBI57_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="BsaG protein"
                     /protein_id="HCD1823581.1"
                     /translation="IVVGVLMFIFNVYFGLEVLGDHSWFYLPITYATCYVYMFIEGSI
                     PVTLTLAIYIIITLLLAVLLFKGFNKWSGRTIKD"
BASE COUNT          479 a          212 c          163 g          393 t
ORIGIN      
        1 tattaacaac attaggaaca ggtttacatt cttcatactt aggattaaat acaaataaag
       61 ttgttaaaac agcaaaagca gaagaaaaaa tgacaaatgg tcaattgtgg aaaaaagtta
      121 aagattcatt aattgattca aatattatta gtggtaacga aaatgaagag attacagtaa
      181 catatgttaa taaaaccgga tattcttcaa gtgtttctgc ctatggtaat aataatgacg
      241 atttttcaag tactccaagt aatttttcta aacttaagga aattgacttg aaaaaagata
      301 atgtaccgtc agatgatttt aatactactg ttagtggtga agatagttgg aaaacactta
      361 catctaaatt aaaggaaaaa ggtttggtta cagatggaca aacagtaact attcattgta
      421 atgataagtc tgacaataca aaatcatctg tttcaggtaa agtaggagca gatctgacaa
      481 gtggcaatgg aactacattc aaaaaacgct ttattgataa aatcacaatt gattaaacct
      541 taaactcaaa tttccttaat tcataaaacc ttaataacta accctttctt acaaaccacc
      601 tagcccataa tatattaaaa cgtcattttt atttcaatta gcggaaaata aaaaaaccat
      661 tccgcattaa ctttggcaag ttggaatggt taaaaaatat tttagaagcc agcgtttggc
      721 ttgctttgac gacatgttcg cgcatgtcgt cttattttta catttatata ataacacgca
      781 attcaataaa atgtctaatc aataaacctc aattacattc aaattcaaaa actttgcacc
      841 taatattcat cgcttaaata aacattacgt ttaattaacc agacattgtc aatcgcgtct
      901 aatacccctg ctgtccgaat acaaaattat tttgtattca tatttatctg ttctctttcc
      961 tcccttttaa acgcaactaa atttctttta ccattagcgc cccaatcttt aatctttaat
     1021 tgttctaccg ctccatttat taaatccttt aaagagtaaa actgctaata gcaacgtgat
     1081 aataatatag atcgccaatg ttaatgtaac tggtatactc ccttcgataa acatataaac
     1141 gtaacacgta gcatatgtga ttggtaaata gaaccacgaa tgatctccaa gcacttctaa
     1201 tccaaaataa acgttaaaaa taaacattaa aactccgaca acaatag
//
LOCUS       DAHHSC010000339         1244 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.346, whole genome shotgun sequence.
ACCESSION   DAHHSC010000339 DAHHSC010000000
VERSION     DAHHSC010000339.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1244)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1244)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1244
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.346"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            71..532
                     /locus_tag="NBI57_002682"
     CDS             71..532
                     /locus_tag="NBI57_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001668280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01741 family protein"
                     /protein_id="HCD1823582.1"
                     /translation="MTFEEKLSQMYNEIANKISSMIPVEWEKVYAMAYVNERSGEVFY
                     NYTEPRSDELFYYTSVLNKYNISRSEFMDSVYELYKQFDKLRDLFKEEGLEPWTSCEF
                     DFTRDGKLNVSFDYIDWANSEFGQMGREHYYMYKKFGIWPEKEYAINWVKK"
     gene            581..1066
                     /locus_tag="NBI57_002683"
     CDS             581..1066
                     /locus_tag="NBI57_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01741 family protein"
                     /protein_id="HCD1823583.1"
                     /translation="MNFEEKLSQMYNEIANEISGMIPVEWEQVFTIAYVTDQAGEVIF
                     NYTKPGSDELNYYTYIPREYNVSEKVFYDLWTDLYRLFKKLRNAFKEDLEPWTSCEFD
                     FTREGNLKVSFDYIDWIKLGFGPSGKENYYMYKKFGVLPETEYEMEEIREVEKYVKDQ
                     E"
BASE COUNT          500 a          125 c          252 g          367 t
ORIGIN      
        1 atggaagaaa ttaaagaaat cgagcaatat attaaagagc aagatgaagc tgaactatag
       61 gggcgataat atgactttcg aagaaaaact aagtcaaatg tacaatgaaa ttgcaaataa
      121 aattagcagt atgataccgg tagaatggga aaaggtatat gcaatggctt atgtaaatga
      181 aagaagtgga gaagtgttct acaattatac tgaaccaaga agtgatgaat tattttacta
      241 tacgagcgtg ttaaataaat ataatatatc aagatcagaa tttatggact cagtatatga
      301 attatataag caatttgata aactaagaga tttatttaaa gaagaaggac tcgaaccatg
      361 gacatcatgt gaatttgact ttacaagaga cggcaaattg aatgtttcat ttgattatat
      421 tgattgggcg aattcagagt ttggacaaat gggaagagaa cattattaca tgtataaaaa
      481 atttggaatt tggcctgaaa aagaatatgc cataaattgg gtaaaaaaat aaaagattat
      541 gttaaagagc aagatgaagc tgaactatag gggcgataac atgaattttg aagaaaaatt
      601 aagtcaaatg tacaacgaga ttgcgaatga gattagtggg atgataccag tagaatggga
      661 gcaagtattt acaatagctt atgtaactga tcaagctggg gaagtcattt ttaattatac
      721 taaaccaggt agtgatgaat tgaattatta cacatatatc cctagagagt ataatgtctc
      781 tgaaaaagta ttttatgatt tgtggacgga tttatataga ttgtttaaga agttaagaaa
      841 cgcatttaaa gaagatttag aaccatggac atcatgtgaa tttgatttta caagagaggg
      901 taatttgaaa gtatcgtttg attatataga ttggattaaa ttaggttttg gtccatcagg
      961 aaaggaaaac tactatatgt ataaaaagtt tggtgtttta ccagaaacgg aatatgaaat
     1021 ggaagaaatt cgagaagtag agaagtatgt taaagaccaa gagtagcata tatgttatag
     1081 aaggctgtgc aaaatcaccc tcgttttaca tttgactcaa agaagaaggt aaacgataag
     1141 attatttgca acttaaaaag tttattagct tctcggtatc catacatcat atgtataaat
     1201 gagtttaact aattaataaa tcacgatata aatttaaaat tgcc
//
LOCUS       DAHHSC010000340         1226 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.347, whole genome shotgun sequence.
ACCESSION   DAHHSC010000340 DAHHSC010000000
VERSION     DAHHSC010000340.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1226)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1226)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1226
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.347"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            complement(<1..218)
                     /locus_tag="NBI57_002684"
     CDS             complement(<1..218)
                     /locus_tag="NBI57_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1823584.1"
                     /translation="MSEWHINNQSTKPFLIQQAEKQLSIVKPLPNGSFQILTEINLEN
                     GNISNIDHSLLVSVDPKALEISIFDAVN"
     gene            complement(280..564)
                     /locus_tag="NBI57_002685"
     CDS             complement(280..564)
                     /locus_tag="NBI57_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1823585.1"
                     /translation="MNELTKEQKYTIAKFYKLYIERSNNGETETVANFFGDAKDAREN
                     YFCDRDYQDFLTNCQILIQNKYLTDEILDDNIYNISILNKTFIEFEQNFG"
     gene            complement(652..1221)
                     /locus_tag="NBI57_002686"
     CDS             complement(652..1221)
                     /locus_tag="NBI57_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1823586.1"
                     /translation="MNYIKRTIILLILFVVVSPINSPKTIADNKYSEIQDDKFQLQPG
                     DIIVTKGPVMWGFFGHCSIAIDDKTILQIEGPGDKPTTQSFESFKYNYASGKNDWMKV
                     YRCSYPGAGKKAAEWVKKNYENTNHRYLVTLNLNSKKFTYCTKIIYQAYKFGVSEKSV
                     KSYGLHIISPYAIKDNFIDPYKLRLVKAY"
BASE COUNT          394 a          185 c          179 g          468 t
ORIGIN      
        1 taatttacag cgtcaaatat acttatttct aatgctttgg ggtctactga aacaagtaaa
       61 gaatgatcga tgttactaat attgccattc tccaaattta tttctgtgag tatttggaag
      121 ctaccattag gcaatggttt aacaatagac aattgctttt ccgcttgttg tattaaaaaa
      181 ggttttgtag attgattatt aatatgccat tcactcatgt atgtttttca ctcctgcttt
      241 aaaatagggt taaaaagttt atagttgaga cattcatgtt caaccaaaat tttgttcaaa
      301 ttcaataaat gtcttgttta aaatagaaat attgtaaatg ttatcgtcca aaatttcatc
      361 agttaagtat ttgttttgaa ttaaaatttg gcagttagtt aagaagtctt gataatcacg
      421 atcgcaaaaa tagttttcac gtgcatcttt agcatcgcca aaaaagttag cgactgtttc
      481 tgtttctcca ttattcgaac gttcaatata taatttgtaa aatttagcta ttgtatactt
      541 ttgttcttta gttagttcat tcaaaatatt gggcctcctg aaatatcatt tgtaatctat
      601 acccaattta ttgcaaaaca caaactaatt taactatttg atgaaactgt gttaataagc
      661 ttttacaagc cttagtttgt atggatctat aaaattatct ttaattgcat agggtgaaat
      721 aatatgtagt ccataacttt taactgattt ttcacttaca ccaaatttat aagcttggta
      781 gataatttta gtacaatacg taaatttttt gctgttcaaa tttaatgtaa ctagataacg
      841 atgatttgta ttttcatagt ttttcttaac ccattcagcc gcttttttac ctgcaccagg
      901 atagctgcaa cgataaactt tcatccaatc atttttgcca cttgcataat tatatttaaa
      961 agattcgaag gattgtgtag ttggtttgtc gccaggcccc tcaatttgta aaatcgtttt
     1021 atcatcaatc gcgatactac aatgaccaaa aaatccccac atgacagggc cttttgtaac
     1081 aataatatca ccaggttgta attggaattt gtcatcttga atttctgaat acttattatc
     1141 tgcaattgtt tttggtgagt ttatagggga tacgacaacg aataatataa gtaaaattat
     1201 cgttcgttta atatagttca cttaaa
//
LOCUS       DAHHSC010000341         1225 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.348, whole genome shotgun sequence.
ACCESSION   DAHHSC010000341 DAHHSC010000000
VERSION     DAHHSC010000341.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1225)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1225)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1225
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.348"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            60..1121
                     /locus_tag="NBI57_002687"
     CDS             60..1121
                     /locus_tag="NBI57_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIC"
                     /protein_id="HCD1823587.1"
                     /translation="MSNANNADNKQFFSKILNSIGAGVVIALVPNALLGEVLKIFKSG
                     NEILELTYQLVILIQSFMAFIIGVLAAHQFKFNGAGAAIVGTSAMIGSGAVVYSNNSF
                     MLKGIGDIINTSLVVIIACLIYMVLQNKLGSFELIILPVLVPIVSGGIGLITLPYIRK
                     ITQAIGNVIHSFTDLNPLLMSILISVAFSLLMVTPISLVAIATAISLNGLGSGAANLG
                     IVAACVTFLFGSLRVNSIGVNAVLLIGAAKMMIPVYLKNLIISIPLTINGIITGIIAY
                     VLQVKGTPLSAGFGYTGLVGPINAFNRMSGDPTMNIILLALGYFVIPFVSAFIVHELC
                     KKFIPIYSNDIYKFEVPKQ"
BASE COUNT          385 a          150 c          223 g          467 t
ORIGIN      
        1 attgttaaag ttaattttct ataatatgat gagtttaatg ttcaaattgg aggtcgttta
       61 tgagtaacgc aaataatgct gataataaac agtttttcag taagatttta aattctattg
      121 gtgctggtgt tgttattgca cttgttccga atgctttatt aggtgaagta ttaaaaatat
      181 ttaaaagtgg taatgaaatt ttagaattaa cgtatcagtt agtgatacta attcaatcat
      241 ttatggcatt tattattggg gttttagctg ctcatcaatt taaatttaat ggcgcaggtg
      301 ctgctatagt aggtacatca gctatgattg gttcaggtgc tgttgtatat agcaataata
      361 gttttatgtt aaaaggtata ggtgacatta taaatacgag tttggttgtt attattgcat
      421 gtcttatata tatggtatta caaaacaaac ttggatcttt tgaattaatt atattaccag
      481 ttttagtacc tatcgttagt gggggtatcg gtctaatcac attaccttac attcgaaaaa
      541 ttactcaagc aattggaaat gtaattcatt cttttacaga cttaaatccg ttgttaatgt
      601 ctatacttat tagtgtggca ttttcattat taatggtaac accaatatcg ttagtagcga
      661 ttgcaactgc gattagttta aacggactag gaagtggagc agcaaaccta ggtatagtgg
      721 ctgcctgtgt tactttctta tttggctcat tgcgtgtcaa ttctattggt gtaaatgcag
      781 ttttactcat aggagctgca aaaatgatga tcccagttta cttgaaaaat ttaattattt
      841 cgataccatt gactataaat ggaataatta ctggaatcat tgcttacgtt cttcaggtta
      901 aaggaacacc attgtctgct ggatttggat atacaggatt agttggccca ataaatgcat
      961 ttaaccgaat gtcaggtgat ccaacaatga atattatttt attagcactt ggttattttg
     1021 tcataccatt tgtatcggca tttattgttc atgaattgtg taaaaaattc attcctatat
     1081 attctaatga tatttataaa tttgaagttc caaaacaata attgatagct tgatgacaag
     1141 aataagtttt aatatatgat aaatataggc gttactgtca ataagatatt gatagtagcg
     1201 cttttttgat tgattttaaa atgtg
//
LOCUS       DAHHSC010000342         1225 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.349, whole genome shotgun sequence.
ACCESSION   DAHHSC010000342 DAHHSC010000000
VERSION     DAHHSC010000342.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1225)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1225)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1225
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.349"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            <1..336
                     /locus_tag="NBI57_002688"
     CDS             <1..336
                     /locus_tag="NBI57_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HCD1823588.1"
                     /translation="DIFVEEDKYINAKYIQQVMDENYPGISFDTIYRNLHLFKDLGII
                     ENTELDGEMKFRIACTNHHHHHFICEKCGDTKVIDYCPIDQIKLSLPGVNIHKHKLEV
                     YGVCESCQD"
     gene            612..1211
                     /locus_tag="NBI57_002689"
     CDS             612..1211
                     /locus_tag="NBI57_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HCD1823589.1"
                     /translation="MAFELPKLPYAFDALEPHFDKETMEIHHDRHHNTYVTKLNAAVE
                     GTDLESKSIEEIVANLDSVPANIQTAVRNNGGGHLNHSLFWELLSPNSEEKGTVVEKI
                     KEQWGSLEEFKKEFADKAAARFGSGWAWLVVNNGQLEIVTTPNQDNPLTEGKTPILGL
                     DVWEHAYYLKYQNKRPDYIGAFWNVVNWEKVDELYNATK"
BASE COUNT          452 a          168 c          216 g          389 t
ORIGIN      
        1 gatatttttg tcgaagaaga taagtatata aacgcaaagt atatacaaca agttatggat
       61 gaaaattatc ctggaatttc attcgacaca atatatagaa acctgcactt atttaaagat
      121 ttagggatta ttgaaaatac agaacttgat ggtgaaatga agtttagaat cgcttgtaca
      181 aaccatcatc atcatcattt tatctgtgaa aagtgtggag atacaaaggt aatagattat
      241 tgtccaatag atcagataaa gttatcacta cctggtgtta atattcacaa acacaaactt
      301 gaagtttatg gtgtatgtga gtcttgccaa gattaatata aagaaatgag atttatgcac
      361 atttggtcag atgtatgcat aaatctcatt ttttaaatta tagaagataa aggttcattt
      421 catactccgt tcgtatgatt ttatggcaaa tctgttaact ttttatatac aaaatgttta
      481 attatttttg atttgagtta tattaaatga gtagagaaaa taataagtag tagaatttaa
      541 gcaaattctt tgttgaacca gtcactgctt gttattattt acttacagac attttaggag
      601 gatgattatt tatggctttt gaattaccaa aattaccata cgcatttgat gcattagaac
      661 cacattttga caaagaaact atggaaattc accatgacag acatcataac acgtatgtta
      721 cgaaattaaa tgctgcagta gaaggtacag atttagaatc taaatctatt gaagaaattg
      781 ttgctaattt agacagtgta ccagctaaca tccaaactgc tgtacgtaat aatggcggtg
      841 gacatttaaa ccattcatta ttctgggagt tactttcacc aaactcagaa gaaaaaggta
      901 ctgtagtaga aaaaattaaa gaacaatggg gttctttaga agaatttaaa aaagaatttg
      961 ctgacaaagc agctgcacgc tttggttcag gttgggcttg gttagtcgta aacaatggcc
     1021 agttagaaat tgtgactaca ccaaaccaag ataatccatt aactgagggt aaaacaccta
     1081 ttttaggttt agacgtatgg gaacacgctt attacctaaa atatcaaaac aaacgccctg
     1141 actacattgg cgcattttgg aatgtagtta actgggaaaa agttgacgaa ttatataatg
     1201 caacaaaata aagtatgatt acttg
//
LOCUS       DAHHSC010000343         1172 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.350, whole genome shotgun sequence.
ACCESSION   DAHHSC010000343 DAHHSC010000000
VERSION     DAHHSC010000343.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1172)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1172)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1172
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.350"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            complement(209..1102)
                     /locus_tag="NBI57_002690"
     CDS             complement(209..1102)
                     /locus_tag="NBI57_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HCD1823590.1"
                     /translation="MKKTILLTMTTLTLFSMSPNSAQAYTNDSKTLEEAKRAHPNAQF
                     KVNKDTGAYTYTYDKNNTPNNNHQNQSRTNDNHQHANQRDFNNNQYHSSLSGQYTHIN
                     DAIDSHTPPQTSPSNPLTPAIPNVEDNDDELNNAFSKDNKGLITGIDLDELYDELQIA
                     EFNDKAKTADGKPLALGNGKIIDQPLITSKNNLYTAGQCTWYVFDKRAKDGHTISTFW
                     GDAKNWAGQASSNGFKVDRHPTRGSILQTVNGPFGHVAYVEKVNIDGSILISEMNWIG
                     EYIVSSRTISASEVSSYNYIH"
BASE COUNT          330 a          183 c          220 g          439 t
ORIGIN      
        1 tgaaaatcta ataaactaca aaaaatagtt tgattacata atgattcttg aaaaatgtcg
       61 gttaacttta aggtgacaag ttattgcaat tttgaaggta aacatcatat ttaataatcg
      121 aataacatac aaaataaagc gaccaatttt gcagtgttac gaattgtaaa ctgcgaactg
      181 gtcgcttttt attgatgtca tgattaattt aatggatgta attatatgat gaaacttctg
      241 aagcagagat ggttcttgat gaaacgatat attcaccaat ccagttcatt tctgaaatta
      301 gaatacttcc atcaatatta actttttcaa cgtaggctac atgaccaaat ggaccattta
      361 ctgtttgtaa aattgatcct cgtgttgggt gtctatctac tttgaagcca ttgcttgaag
      421 cttggcctgc ccagttttta gcatctcccc aaaatgtact aatcgtgtgt ccatctttgg
      481 cacgtttatc aaagacatac catgtacatt gtccagcagt atataagttg ttcttacttg
      541 tgataagagg ctgatcaatg attttaccgt tacctaatgc taaaggttta ccgtcagcag
      601 tctttgcttt gtcattaaat tcggcgattt gtaattcgtc atacaattcg tctaaatcga
      661 tgcctgtaat aagccctttg ttatcttttg aaaaagcgtt atttaattca tcgtcattgt
      721 cttcgacatt cggtattgct ggtgtcaaag gattgcttgg tgacgtttga ggcggtgtgt
      781 gtgaatcaat tgcgtcatta atgtgcgtat actgaccact taatgaagaa tggtactgat
      841 tgttgttaaa atcacgttga tttgcgtgtt gatgattgtc gtttgtacgt gactggtttt
      901 gatgattgtt gtttggcgtg ttgtttttgt catatgtata agtatacgcg ccggtgtctt
      961 tattcacttt gaactgtgcg tttgggtgtg ctctctttgc ttcttctaat gttttgctat
     1021 cattcgtata tgcttgagcc gagttaggcg acatactaaa taaagtaaga gttgtcatcg
     1081 tcagtaaaat tgttttcttc ataataacca tttaatcctt tatgtattta atttaatttt
     1141 agtatacaca tttatattac aaaaatgaat gg
//
LOCUS       DAHHSC010000344         1165 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.351, whole genome shotgun sequence.
ACCESSION   DAHHSC010000344 DAHHSC010000000
VERSION     DAHHSC010000344.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1165)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1165)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1165
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.351"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            <1..219
                     /locus_tag="NBI57_002691"
     CDS             <1..219
                     /locus_tag="NBI57_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002505096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HCD1823591.1"
                     /translation="EKPKIKNYISGGCYIFNKEIIKKVPKNKNLKMTDFLEKLINDNI
                     SISSYVHNGVWIDAGTWEDLKKAKSIFL"
     gene            231..>1165
                     /locus_tag="NBI57_002692"
     CDS             231..>1165
                     /locus_tag="NBI57_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000263482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HCD1823592.1"
                     /translation="MWDIEVSSSDISQKDIEIVCETMKSNWLSMGPKVKEFESMWAKE
                     LGINYISAVSSGTAALHLACLCCEFTYGDEVIVPALTFVATANAVKYTGATPIISDIE
                     SKDNLILSIEEIRKNITSKTKGIIIVHYAGYTYNIEEISKLAKEYNLYLIEDAAHAIT
                     SSYNNKSLGTYGDLACFSFYPNKNITTGEGGVIATNNKDFHEKIRSLRTHGMTTETWN
                     RYNSSISTYDVNNLGYNYRMDDLRASLGISQFKNMKRNKIHRKNLVNKYRELLLGYKE
                     ITIPFLTNNTDSSNYIFVIFVKNPILKEKMITDLR"
BASE COUNT          469 a          138 c          182 g          376 t
ORIGIN      
        1 gaaaaaccta aaataaaaaa ctatatcagt ggtggatgct acatctttaa taaagaaata
       61 ataaaaaaag tacctaaaaa taaaaatctt aaaatgactg attttttaga aaaattaatt
      121 aacgataata tttctatttc ttcttatgtt cataatggag tttggataga cgcaggaact
      181 tgggaagatt taaaaaaagc taaatccata tttttatagg aggatatatt atgtgggaca
      241 ttgaagtttc atcatcagat ataagtcaga aagatattga aattgtatgt gaaactatga
      301 aatctaattg gttaagtatg ggaccaaaag taaaagaatt tgaaagcatg tgggcaaaag
      361 aacttggtat aaattacata tctgctgtat ctagtggaac tgcagcatta catttagctt
      421 gtttatgttg cgagttcact tatggagatg aagttattgt ccccgcatta acatttgtag
      481 ctacagcaaa cgctgtaaaa tataccgggg caactcctat aatatctgat atagaatcta
      541 aggataattt aatattgtca attgaagaaa tacgaaaaaa tataacatcc aaaactaaag
      601 gaattattat cgttcattac gctggatata catacaatat tgaagaaatt tctaaattag
      661 caaaagaata caatctatat ttaattgaag atgctgcaca tgctattact agtagttaca
      721 acaataaatc tttaggtact tatggagatc tagcttgttt tagtttttat cctaataaaa
      781 atataacaac aggtgaaggc ggagtaattg ctactaacaa taaggatttt catgaaaaaa
      841 tccgttcttt aagaacacat ggtatgacta cagagacatg gaatcgatat aattccagta
      901 tttctacata tgatgtaaat aacttaggtt acaactatag aatggatgat ctacgtgctt
      961 ctttaggtat ttctcaattt aaaaatatga aaagaaacaa aatacacaga aagaatttag
     1021 taaacaaata tagagaatta ttgttgggtt ataaagaaat aactattcca tttctaacaa
     1081 ataatactga tagttctaat tatatttttg ttatatttgt aaaaaatcct attttgaaag
     1141 aaaaaatgat tactgatctt agaaa
//
LOCUS       DAHHSC010000345         1165 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.352, whole genome shotgun sequence.
ACCESSION   DAHHSC010000345 DAHHSC010000000
VERSION     DAHHSC010000345.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1165)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1165)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1165
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.352"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            complement(<1..372)
                     /locus_tag="NBI57_002693"
     CDS             complement(<1..372)
                     /locus_tag="NBI57_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1823593.1"
                     /translation="METINNLAMATFQLVILFTVAKFISFVKFNLRDYFIIVGIIIPT
                     MFLYYFYGSRAVLIPTFSSIIFLFFKLKYYAIVTILVTMIIMYLSNFATVGLFLTLRK
                     YTTDPAILLPLYILSFSSVSLL"
     gene            complement(402..545)
                     /locus_tag="NBI57_002694"
     CDS             complement(402..545)
                     /locus_tag="NBI57_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001094921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic lactone autoinducer peptide"
                     /protein_id="HCD1823594.1"
                     /translation="MNTLVNMFFDFIIKLAKAIGIVGGVNACSSLFDEPKVPAELTNL
                     YDK"
     gene            complement(548..1111)
                     /locus_tag="NBI57_002695"
     CDS             complement(548..1111)
                     /locus_tag="NBI57_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator ArgB-like protein"
                     /protein_id="HCD1823595.1"
                     /translation="MNYFDNKIDQFATYLQKRNNLDHIQFLQVRLGMQIIVGNFFKIL
                     VTYSISIFLSVFLFTLVTHLSYMLIRYNAHGAHAKSSILCYIQSILTFVFVPYFLINI
                     DINFTYLLALSIIGLISVVIYAPAATKKQPIPIKLVKRKKYLSIIMYLLVLILSLIIH
                     PFYAQFMLLGILVESITLLPIFFPKED"
BASE COUNT          467 a          155 c          156 g          387 t
ORIGIN      
        1 taataatgaa actgatgaaa aagaaagtat atataatggt aagagtattg caggatcagt
       61 agtgtatttt cttaaagtta aaaacaaacc tactgttgca aaattactca aatacattat
      121 gatcatggtc actaaaattg ttactattgc atagtacttt aatttaaaga ataaaaatat
      181 gatagatgaa aaagttggaa tcaatacggc tctacttcca tagaagtaat acaaaaacat
      241 ggttggaatt ataattccaa ctataataaa ataatccctt aaattaaatt taacaaagga
      301 aatgaatttg gctactgtaa aaagtattac taattgaaaa gtggccatag ctaagttgtt
      361 tattgtttcc atatgctaca cactttctaa aataactttt actatttgtc gtataaattc
      421 gttaattcag cgggtacttt aggttcatca aataaactgc tgcaagcgtt tacgccaccg
      481 acaattccga ttgctttagc taatttaatt ataaaatcaa aaaacatatt aacaagtgta
      541 ttcatgatta atcctcctta gggaaaaaaa taggtaataa tgtaatagat tccactaaaa
      601 ttcctaataa cataaattga gcataaaatg gatgtattat taaggataga atcaaaacta
      661 ataaatacat aattatagat aaatactttt tacgttttac taattttatt ggtataggtt
      721 gctttttcgt tgctgcaggt gcataaataa caacggaaat caatcctata atagataagg
      781 ccagtaaata agtaaaatta atgtctatat taattagaaa ataaggtaca aatacaaatg
      841 ttaaaatact ttgtatgtaa caaagtatcg aagactttgc atgagcacca tgtgcattat
      901 agcgtattaa catataggat aagtgtgtaa ctaaggtaaa caaaaataca cttaagaata
      961 tagaaatact atatgttaca agtattttaa aaaaattacc aactataatt tgcatcccta
     1021 aacgtacttg caaaaattga atatgatcta agttatttct cttttgaaga tacgtggcaa
     1081 actggtcaat tttgttatca aaataattca attttacacc actctcctca ctgttattat
     1141 acgatttagt acaatctttt atcat
//
LOCUS       DAHHSC010000346         1152 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.353, whole genome shotgun sequence.
ACCESSION   DAHHSC010000346 DAHHSC010000000
VERSION     DAHHSC010000346.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1152)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1152)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1152
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.353"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            252..>1152
                     /locus_tag="NBI57_002696"
     CDS             252..>1152
                     /locus_tag="NBI57_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001623710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAP domain-containing protein"
                     /protein_id="HCD1823596.1"
                     /translation="MKFKSLITTTLALGVIASTGANFNTNEASAAAKPLDKSSSTLHH
                     GHSNIQIPYTITVNGTSQNILSSLTFNKNQNISYKDIENKVKSVLYFNRGISDIDLRL
                     SKQAEYTVHFKNGTKRVIDLKSGIYTADLINTSDIKAISVNVDTKKQPKDKAKANVQV
                     PYTITVNGTSQNILSNLTFNKNQNISYKDLEGKVKSVLESNRGITDVDLRLSKQAKYT
                     VNFKNGTKKVIDLKSGIYTANLINSSDIKSININVDTKKHIENKAKRNYQVPYSINLN
                     GTSTNILSNLSFSNKPWTNYKNLT"
BASE COUNT          503 a          151 c          157 g          341 t
ORIGIN      
        1 aaatataatc ggaattaaga aatgaagatt gttatgacat ttaagtttga aacttggtat
       61 aaacaaaatc aaacaacatt ttaggtattg taaattagtt agttaatcaa aattaaaaga
      121 acttttaaca gtgttaaaaa attattgtta aagataattt attattaata ttcagttaat
      181 tcaaaaaata gagaaagtct ggctataata aagttgcaat cacgaattac ataaaaaagg
      241 agagataatt tatgaaattt aagtcattga ttacaacaac attagcatta ggcgttatag
      301 catcaacagg agcaaacttt aatactaacg aagcatctgc cgcagctaag ccattagata
      361 aatcatcaag tacattacac catggacatt ctaacatcca gattccatat acaattactg
      421 tgaacggtac aagccaaaac attttatcaa gcttaacatt taataagaat caaaatatta
      481 gttataaaga tatagagaat aaagttaaat cagttttata ctttaataga ggtattagtg
      541 atatcgattt aagactttca aagcaagcgg aatatacggt tcattttaaa aatggaacaa
      601 aaagagttat cgatttgaaa tcaggtatct acacagctga cttaatcaat acaagtgaca
      661 ttaaagctat cagtgttaac gtagatacta aaaagcaacc taaagataaa gctaaagcaa
      721 atgttcaagt gccatataca atcacagtga acggcacaag ccaaaacatt ttatcaaacc
      781 taacatttaa taaaaatcaa aatattagtt acaaagattt agagggtaaa gttaaatcag
      841 ttttagaatc aaatagaggt attactgatg ttgatttaag actttcgaag caagcgaaat
      901 atacagttaa ttttaaaaat ggaacgaaga aagttatcga tttgaaatca ggtatttaca
      961 cagcgaattt aatcaattca agtgatatta aaagtatcaa tattaacgta gatacaaaaa
     1021 aacatatcga aaataaagct aaaagaaact atcaagttcc atattcaatt aatctaaatg
     1081 gtacatctac aaacatttta tcgaatcttt cattttcaaa taaaccttgg acaaattaca
     1141 aaaatttaac ta
//
LOCUS       DAHHSC010000347         1151 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.354, whole genome shotgun sequence.
ACCESSION   DAHHSC010000347 DAHHSC010000000
VERSION     DAHHSC010000347.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1151)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1151)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1151
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.354"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            78..328
                     /locus_tag="NBI57_002697"
                     /pseudo
     CDS             78..328
                     /locus_tag="NBI57_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016187510.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sphingomyelin phosphodiesterase"
     gene            650..829
                     /locus_tag="NBI57_002698"
     CDS             650..829
                     /locus_tag="NBI57_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1823597.1"
                     /translation="MKFKTTKECKSNNIFKRSQEINNRESEKGCLWGISMLILLFLLI
                     LFGITACSSSIHFIN"
BASE COUNT          428 a          133 c          157 g          433 t
ORIGIN      
        1 tcgatttaat aattaattta aatttagtta atcaattttg catctatttt gttgtaagct
       61 atataaaagg agtgataatg atggtaaaaa aaacaaaatc caattcacta aaaaaagttg
      121 caacacttgc attagcaaat ttattattag ttggtgcact tactgacaat agtgccaaag
      181 ccgaatctaa gaaagatgat actgatttga agttagttag tcataacgtt tatatgttat
      241 cgaccgtttt gtatccaaac tggagacttt taacataaaa ttacttatca ttcaaaaagt
      301 aaaacagcat aatatcaagg tttataactt tatcattatc aataatacct catataaaat
      361 aaaattttag ggacttttta gggactttaa atttaaaatt acaagtttaa tagaaacatc
      421 aaaataatca catgtttgtg tggaatgtac accccaaaag ctagactgaa aaatctattt
      481 tttgaggtgt atttttatag gtaaatataa taaattagag tagacaactc agaattccaa
      541 ttttataata atattgcttg acatatcaaa ctagatagta ctattttgaa tatattatta
      601 taatcaaaaa ttcattgtaa acttttagac aaaaggaagt aataaaaacg tgaaatttaa
      661 aacaactaaa gaatgtaaaa gtaataatat ctttaaaaga agtcaagaaa ttaataatag
      721 agaaagtgaa aagggttgtt tatggggcat tagcatgttg attctacttt tcttattgat
      781 tctgtttgga ataactgctt gttcatcaag cattcacttt attaattaga tttttttact
      841 tggaggtatc atgtgaagaa ccatacaaat ataattaata tcttattagt tatagtcaac
      901 tcattaactc attttctaac tctaaacacc tcatttttta atagttcagc atcggatttc
      961 tgttttatca taggggctat atttttcttg atcggaattt ttgttgcaat atacggtatg
     1021 aagcgagcaa catattggtt aaacttattg attttattta ccaatatttt ttattttcta
     1081 cacttctgtg ttttactttt gttaaaatat ataggattta aattatttat ttatttatga
     1141 agggtgtgta t
//
LOCUS       DAHHSC010000348         1145 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.355, whole genome shotgun sequence.
ACCESSION   DAHHSC010000348 DAHHSC010000000
VERSION     DAHHSC010000348.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1145)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1145)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1145
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.355"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            <1..292
                     /locus_tag="NBI57_002699"
     CDS             <1..292
                     /locus_tag="NBI57_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HCD1823598.1"
                     /translation="KINDRYMSDVKQDFIFHNELPQDSLMIIKTLNHEHGSIQHLLPI
                     SDQLVYIEKEILDELDKTNYPKTELAVQEETGRTFELKLNHDEVKYICFKPS"
     gene            363..785
                     /locus_tag="NBI57_002700"
     CDS             363..785
                     /locus_tag="NBI57_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HCD1823599.1"
                     /translation="MSNSQAIQAIENVLATSKVGVLSTAYNNKPNSRYMVFYNDGLTL
                     YTKTNIHSAKVKEIKDNPAAYVLLGYNDTTNRSFVEMEATIEVVTDQKVIDWLWETQD
                     KSFFSSKEDPELCVLKVTPQSVKLMNDKSLDTPIKIDL"
BASE COUNT          428 a          157 c          182 g          378 t
ORIGIN      
        1 taaaattaat gatcgatata tgtcagacgt aaaacaagat ttcattttcc ataatgaatt
       61 acctcaagac tctttgatga ttattaaaac attgaatcat gaacatggtt caattcaaca
      121 tttgcttcca ataagcgatc aacttgttta tatagaaaaa gaaattttag atgaattaga
      181 caaaacgaat taccctaaaa cggagcttgc agttcaagaa gaaactggtc gtacatttga
      241 actcaagtta aatcacgacg aggttaaata tatttgcttt aaaccaagct aaatactaac
      301 agtcctcttg tgtttagttt cttacgttaa aggctattta tatcataagg agatgatatg
      361 taatgagtaa ctcacaagca attcaagcaa ttgaaaacgt gttagcaacg tcaaaagttg
      421 gtgtattatc aactgcatat aataacaaac ctaatagtag atatatggtc ttttataatg
      481 atggtcttac tttatatact aaaacgaata tccattctgc taaggtcaaa gaaattaaag
      541 ataatccagc agcatatgtt ttgttaggct ataacgacac aactaatcgt agtttcgttg
      601 aaatggaagc cacgatagaa gtagttactg atcaaaaagt aatcgattgg ttatgggaga
      661 ctcaagataa aagctttttc agttcaaaag aagacccaga actttgtgtt ttaaaggtga
      721 caccacaatc tgttaaattg atgaatgaca aatcattaga tacacctatc aaaatcgatt
      781 tataacacaa agtatatata ggaaataact tttatgaatt ctagatataa caatgttaaa
      841 tacttaaaat aactcgctat aattaaagtg tttaatatgt ttacaatcca atttgcaatg
      901 tattgttttt agacacactt tattcacatt taacgtgatt agggtgtgtc tttttaaatt
      961 gattttatgt aatataaata ctgcatttgc aaactgttgc acttttaggt ataacagaat
     1021 taactacatt taaggagatt gatgaaccat gaaaaagaaa aaaggttttg gtcttggtat
     1081 tagtttaatc gccatcatgt taattgtatg tattgtatta gtaatcatga tgatgactgg
     1141 cggaa
//
LOCUS       DAHHSC010000349         1133 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.356, whole genome shotgun sequence.
ACCESSION   DAHHSC010000349 DAHHSC010000000
VERSION     DAHHSC010000349.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1133)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1133)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1133
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.356"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            91..1050
                     /gene="hyl"
                     /locus_tag="NBI57_002701"
     CDS             91..1050
                     /gene="hyl"
                     /locus_tag="NBI57_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hemolysin"
                     /protein_id="HCD1823600.1"
                     /translation="MKTRIVSSVTTTLLLGSILMNPVANAADSDINIKTGTTDIGSNT
                     TVKTGDLVTYDKENGMHKKVFYSFIDDKNHNKKLLVIRTKGTIAGQYRVYSEEGANKS
                     GLAWPSAFKVQLQLPDNEVAQISDYYPRNSIDTKEYMSTLTYGFNGNVTGDDTGKIGG
                     LIGANVSIGHTLKYVQPDFKTILESPTDKKVGWKVIFNNMVNQNWGPYDRDSWNPVYG
                     NQLFMKTRNGSMKAAENFLDPNKASSLLSSGFSPDFATVITMDRKASKQQTNIDVIYE
                     RVRDDYQLHWTSTNWKGTNTKDKWTDRSSERYKIDWEKEEMTN"
BASE COUNT          431 a          169 c          200 g          333 t
ORIGIN      
        1 atatttgata tgtctcaact gcaatattct aaattgacat attgattatt gtttcctcaa
       61 atcgttaaaa aaaatagaag gatgatgaaa atgaaaacac gtatagtcag ctcagtaaca
      121 acaacactat tgctaggttc catattaatg aatcctgtcg ctaatgccgc agattctgat
      181 attaatatta aaaccggtac tacagatatt ggaagcaata ctacagtaaa aacaggtgat
      241 ttagtcactt atgataaaga aaatggcatg cacaaaaaag tattttatag ttttatcgat
      301 gataaaaatc acaataaaaa actgctagtt attagaacga aaggtaccat tgctggtcaa
      361 tatagagttt atagcgaaga aggtgctaac aaaagtggtt tagcctggcc ttcagccttt
      421 aaggtacagt tgcaactacc tgataatgaa gtagctcaaa tatctgatta ctatccaaga
      481 aattcgattg atacaaaaga gtatatgagt actttaactt atggattcaa cggtaatgtt
      541 actggtgatg atacaggaaa aattggcggc cttattggtg caaatgtttc gattggtcat
      601 acactgaaat atgttcaacc tgatttcaaa acaattttag agagcccaac tgataaaaaa
      661 gtaggctgga aagtgatatt taacaatatg gtgaatcaaa attggggacc atatgataga
      721 gattcttgga acccggtata tggcaatcaa cttttcatga aaactagaaa tggttctatg
      781 aaagcagcag agaacttcct tgatcctaac aaagcaagtt ctctattatc ttcagggttt
      841 tcaccagact tcgctacagt tattactatg gatagaaaag catccaaaca acaaacaaat
      901 atagatgtaa tatacgaacg agttcgtgat gactaccaat tgcattggac ttcaacaaat
      961 tggaaaggta ccaatactaa agataaatgg acagatcgtt cttcagaaag atataaaatc
     1021 gattgggaaa aagaagaaat gacaaattaa tgtaaattat ttgttcatgt acaaataaat
     1081 ataatttata actttagccg ataacttcag aaatgatgtt tttcggctaa ttt
//
LOCUS       DAHHSC010000350         1093 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.357, whole genome shotgun sequence.
ACCESSION   DAHHSC010000350 DAHHSC010000000
VERSION     DAHHSC010000350.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1093)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1093)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1093
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.357"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            complement(62..580)
                     /locus_tag="NBI57_002702"
     CDS             complement(62..580)
                     /locus_tag="NBI57_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GrpB family protein"
                     /protein_id="HCD1823601.1"
                     /translation="MYSNVQPFINSDLTNCFQSQYTEIESLLFNLLDSPVKYTKHIGG
                     THHFKYSTEPILDILVGVENLHDITSLDEKRLNYVGFYRLHHKYTKKVMMAKFNNFHE
                     LKQQVRLHIIQMNTQTFESYLALDYLLSTNDDIALQFNTKKHQILQHSLTIRQYEMQK
                     QQYFEKLCKKYL"
     gene            700..>1093
                     /locus_tag="NBI57_002703"
     CDS             700..>1093
                     /locus_tag="NBI57_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EMYY motif lipoprotein"
                     /protein_id="HCD1823602.1"
                     /translation="MKKIVIIAVLAILFVVISACGNKEKEAQHQFTKQFKDVEQKQKE
                     LQHVMDNIHLKEIDHLSKTDTTDKNSKEFKALQEDVKNHLIPKFEAYYKSAKNLPDDT
                     MKVKKLKKEYMTLANEKEDAIYQLKKFIG"
BASE COUNT          420 a          124 c          171 g          378 t
ORIGIN      
        1 tgcgaaaatt gaaaagaaag tattagaatt agtataatta taaaatacgg cattattgtc
       61 gttataagta ttttttacat agtttttcaa agtattgttg cttttgcatc tcatattgtc
      121 taattgttaa gctatgttgc aatatttggt gcttttttgt attgaattgc aaagcaatat
      181 catcattagt tgataagagg taatcaagtg caagataaga ttcaaatgtt tgggtattca
      241 tttgaatgat atgtagacgc acctgttgtt ttagttcatg aaaattgtta aacttcgcca
      301 tcataacttt cttagtatat ttatgatgca aacgataaaa ccctacataa tttaagcgtt
      361 tttcatctaa ggatgtaata tcatgcaaat tttctacacc tactaaaata tctaaaattg
      421 gctctgttga atatttaaaa tgatgcgtac cgccaatatg ttttgtatat tttactgggc
      481 tgtctaagag gttgaataat aatgattcaa tttcagtgta ttgtgattga aaacaattag
      541 ttaaatcact attaatgaat ggttgaacat ttgaatacat gataaactcc tttgatattg
      601 aaaattaatt taatcacgat aaagtctgga atactataac ataattcatt ttcataataa
      661 acatgttttt gtataatgaa tctgttaagg agtgcaatca tgaaaaaaat tgttattatc
      721 gctgttttag cgattttatt tgtagtaata agtgcttgtg gtaataaaga aaaagaggca
      781 caacatcaat ttactaagca atttaaagat gttgagcaaa aacaaaaaga attacaacat
      841 gtcatggata atatacattt gaaagaaatt gatcatctaa gtaaaactga tacaactgat
      901 aaaaatagta aagaatttaa ggcactacaa gaagatgtta aaaaccatct catacctaaa
      961 tttgaagcat attataagtc agcaaaaaat ttgcctgatg atacaatgaa agttaagaaa
     1021 ttaaaaaaag aatatatgac gcttgcaaat gagaaggagg atgcgatata tcaattaaaa
     1081 aaattcatag gtt
//
LOCUS       DAHHSC010000351         1085 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.358, whole genome shotgun sequence.
ACCESSION   DAHHSC010000351 DAHHSC010000000
VERSION     DAHHSC010000351.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1085)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1085)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1085
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.358"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            35..>1085
                     /locus_tag="NBI57_002704"
     CDS             35..>1085
                     /locus_tag="NBI57_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCD1823603.1"
                     /translation="MAKSCLHILTNNEYATTRCQDGIVLFWPIDGEIELQKFRKSKII
                     EDDIYIINHLDVFSIKNNKKTIMLYLSSDWFAELGFTFFNYHYTAKLIKSSYNLKCLL
                     LKLTYRYLDNQPLNDADIRKLQDIIKIIAKEASMDKKIAQNQYRYAYYGDLRDELEYI
                     YQNVNQRLTLKSVADKLFVSKSNLSSQFHLLMGMGFKKYIDTLKIGKSIEILLTTDST
                     ISNISEHLGFSSSSTYSKMFKSYMDITPNEYRNLSKYNKCLMLKPEPLVGKMVQEVKE
                     IILNYIEHYKNHLTDVIHIDEDKFETPKLFQTVIQINTYTEMKLVFLEGIFKTLLNKN
                     SQVVFFIMPSILKSKN"
BASE COUNT          426 a          141 c          165 g          353 t
ORIGIN      
        1 aaagtggtac ttaaatatag aaagaggtaa agttatggca aaatcatgct tgcatatact
       61 tactaataat gaatatgcga caacgcgttg ccaagatggc atagtcttat tttggccaat
      121 tgacggggaa atcgaactac aaaaatttcg taaaagtaaa ataattgaag atgatatata
      181 tattattaat catctggatg tatttagtat taagaataat aaaaaaacga tcatgttgta
      241 tttgagtagc gattggtttg cggaattagg ctttactttc tttaattacc actatacagc
      301 aaagttgatt aaatcatcct ataatttgaa atgtctacta ttaaaattga cttatcgata
      361 tcttgataat caacctctca atgacgctga tattagaaaa ttacaggata ttattaaaat
      421 cattgcaaaa gaagcaagta tggataaaaa gattgcgcaa aatcaatatc gatatgccta
      481 ttatggtgat ttacgtgacg agctcgaata tatttatcaa aatgtaaatc aacgattgac
      541 attaaaaagt gtcgctgata aattatttgt ctcaaagtca aatttgtcat cacaattcca
      601 cttacttatg ggcatgggtt ttaaaaaata tattgatact ttgaaaattg gtaaatcgat
      661 tgaaattcta cttactactg atagtactat tagcaacata agtgagcatt taggttttag
      721 tagtagctcc acttactcta aaatgtttaa aagttatatg gatataacac cgaatgaata
      781 tcgtaattta tcaaaatata ataaatgttt aatgctaaag ccagaaccac tagtaggcaa
      841 aatggtgcaa gaagtaaaag aaatcatatt gaattatatt gaacattata aaaaccacct
      901 aactgatgtt atacatattg atgaagacaa atttgaaaca cctaaattgt ttcaaacggt
      961 tattcaaata aatacttata cagaaatgaa attagttttc ttagaaggaa tctttaaaac
     1021 cttattgaat aagaacagtc aagttgtctt tttcatcatg ccatcgattc taaaaagtaa
     1081 aaata
//
LOCUS       DAHHSC010000352         1081 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.359, whole genome shotgun sequence.
ACCESSION   DAHHSC010000352 DAHHSC010000000
VERSION     DAHHSC010000352.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1081)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1081)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1081
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.359"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            complement(87..1073)
                     /locus_tag="NBI57_002705"
     CDS             complement(87..1073)
                     /locus_tag="NBI57_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylinositol-specific phospholipase C"
                     /protein_id="HCD1823604.1"
                     /translation="MKKCIKTLFLSIILVVMGGWYHSAHASDSLSKSPENWMSKLDES
                     KHLTEINMPGSHDSGSFTLTDPVKSVWAKTQGKDYLTQMKSGVRFFDIRGRASADNMI
                     SVHHGMVYLHHELGKFLDDAKYYLSAYPNETIVMSMKKDYDSDSKVTKTFEEIFREYY
                     YNNPQYQNLFYTGSNANPTLKETKGKIVLFNRMGGTYIKSGYGADTSGIQWADNATFE
                     TKINNGSLNLKVQDEYKDYYDKKVEAVKNLLAKAKTDSNKDNVYVNFLSVASGGSAFN
                     STYNYASHINPEIAKTIKENGKARTGWLIVDYAGYPWPGYDDIVSEIIDSNK"
BASE COUNT          333 a          222 c          131 g          395 t
ORIGIN      
        1 ttacttattt tcaaaataaa aacgagtagt gaaaataatt agaatctaac tattttcata
       61 ctcgtcttaa tatcattatt gaatccttat ttattactat ctataatttc acttacgata
      121 tcatcatatc caggccacgg atatcctgca tagtcaacaa tcagccaacc agttctagct
      181 ttcccgtttt ctttaatcgt ttttgcaatt tcaggattta tatgtgatgc atagttataa
      241 gtactattaa atgcgctacc tccagacgct acactcaaga aattcacata tacattgtct
      301 ttgttactat ccgttttagc tttagccaat aaatttttaa cagcttcaac ttttttatca
      361 tagtaatctt tatactcatc ttgtactttt aaatttaagc taccattatt aattttcgtt
      421 tcaaatgtcg cattgtctgc ccattgaata cctgacgtgt cagcaccata accactcttt
      481 atgtatgtac cccccattct attgaatagg acaattttac ctttcgtttc ttttaaagta
      541 ggattcgcat tacttcctgt gtaaaaaaga ttctgatatt gcgggttatt ataataatat
      601 tctctaaaaa tctcttcaaa tgtcttcgta actttagaat cgctatcgta gtcctttttc
      661 atagacatca caattgtttc gtttggataa gcactcaagt aatatttagc atcatctaaa
      721 aattttccta gttcatgatg caaataaacc atgccgtgat gaactgaaat catattatca
      781 gcacttgctc tacctctaat atcaaaaaac ctgactcccg acttcatttg agtaaggtaa
      841 tctttgcctt gagtctttgc ccaaaccgat tttaccggat cagttaaagt gaatgagcca
      901 ctatcatgtg agcctggcat attaatctca gttaaatgtt tgctttcatc aagtttactc
      961 atccaatttt ctggactttt actcaacgaa tctgacgcat gtgctgaatg ataccaacca
     1021 cccatcacta ctaaaatgat acttaaaaac aaagtcttaa tacacttttt cataatatca
     1081 a
//
LOCUS       DAHHSC010000353         1077 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.360, whole genome shotgun sequence.
ACCESSION   DAHHSC010000353 DAHHSC010000000
VERSION     DAHHSC010000353.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1077)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1077)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1077
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.360"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            <1..228
                     /locus_tag="NBI57_002706"
     CDS             <1..228
                     /locus_tag="NBI57_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1823605.1"
                     /translation="EDEQVIIYEYIPEDDISNGKGSVTFNKKDAEVIDFSLSEIENEE
                     YFMLYRNKSFSVVRDFIEKQEFPENYKIAWY"
     gene            304..507
                     /locus_tag="NBI57_002707"
                     /pseudo
     CDS             304..507
                     /locus_tag="NBI57_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002463904.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
     gene            592..944
                     /locus_tag="NBI57_002708"
                     /pseudo
     CDS             592..944
                     /locus_tag="NBI57_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000891995.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            951..>1077
                     /locus_tag="NBI57_002709"
     CDS             951..>1077
                     /locus_tag="NBI57_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LSM domain protein"
                     /protein_id="HCD1823606.1"
                     /translation="MNLQSYIGKLVKLTLTNNKILIGKVIDFDDKVDNFDGYNSIE"
BASE COUNT          452 a          111 c          185 g          329 t
ORIGIN      
        1 gaagatgaac aggttataat atatgaatat atacctgaag atgatataag taacggtaaa
       61 ggttcagtaa cttttaataa aaaagatgca gaggttatag atttctcatt atctgaaata
      121 gaaaatgaag aatattttat gttatatcgt aataagtctt tttctgtagt aagagacttt
      181 atcgagaagc aagaatttcc cgaaaattat aaaatagcat ggtattaaag catcctttct
      241 acacagataa agagaaagcg atgctatttt tatatgcttt tttcattatt cgttgaaaaa
      301 gaagagtcac aaaagttaac atatttaaaa gaacttggtg aagatgacga atataaatat
      361 gttgccaaaa tagacagtaa aacatctaaa ttatgtcatt cactcaacgg aaaaatattt
      421 atagttaaag atatgatacc aggtgtgaat gcgccaccta tgcatccttg gtgtagaagt
      481 accacagtgc cacatgttgg caattggtga gacaagttca ttaaagagcg tgaaggtaag
      541 tatcaagtag aagtaaaaga agcaaaatta caagaaaaag ctaaaaaaga aatgcttgat
      601 atgataagta atggtaaaat aaagatgcaa ttgaataatg aaaaacaaca gcgtcatatt
      661 ttaaacacaa atgaatataa taacaagaaa aataattcat ctttcttacc cagttatatt
      721 acaatagatt ctaatgaaat tgaaaaggtg actaaaaaag aatttattga ttttccagtt
      781 ttgtttgatg atgaagggaa tttcagaaaa aaacaagtta tagattacaa aaagataata
      841 ggtaaatctt atgtgaatgg taaatatatg ttgaaacgaa attaggtaaa gttcattatt
      901 ctaaaactgg ttttcatgta gttccgtata ttgaaaagga gtgattaaat atgaatttac
      961 agtcatacat aggcaaatta gtcaaattaa cgcttactaa taataaaata ttaattggaa
     1021 aagtgataga ctttgatgat aaagttgata attttgatgg ttataattcg atagaaa
//
LOCUS       DAHHSC010000354         1076 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.361, whole genome shotgun sequence.
ACCESSION   DAHHSC010000354 DAHHSC010000000
VERSION     DAHHSC010000354.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1076)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1076)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1076
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.361"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            52..576
                     /locus_tag="NBI57_002710"
     CDS             52..576
                     /locus_tag="NBI57_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001641610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1823607.1"
                     /translation="MLYLNKYKERVTSVRIQNRWVVFILFLICSFGVLIGLYQYRHTK
                     TVDLSNLEINDIKLNEKFDKKGYEVNKKIKFDRFKFYNSKAHPDLTVKVREKDNIVKG
                     IILVRDEKIHTNFDGGIGSPINNAIENLGFGYKRTKVGNDFSSVKYIDRDNHLKLNLL
                     YQDLEIKRIEFFSK"
     gene            636..>1076
                     /locus_tag="NBI57_002711"
     CDS             636..>1076
                     /locus_tag="NBI57_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001669025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HCD1823608.1"
                     /translation="MNKITYLNELETALDALPRHLRDQKMYEYERYFYEQELNGVDEG
                     EILKKLKDPQDVAAETKARSVIDYAESKPTFENISRAVAASLSLGILSIFVILIPVSI
                     VGLFVLALFLISLLLLFCPIILLASAISRGIVDSISNVFFAISYS"
BASE COUNT          389 a          122 c          189 g          376 t
ORIGIN      
        1 aattgcaaat tctttgttaa tttttgttaa ggatgaaacg ggaggcacct tatgctatat
       61 ttaaataagt acaaagaaag ggtgacatca gtgcgtattc aaaatcgctg ggttgtgttt
      121 atattatttt taatctgttc ttttggtgta ttaattggtt tatatcaata tcgtcatacg
      181 aaaactgtgg atttgtctaa tcttgaaata aacgatatta aattaaatga gaagtttgat
      241 aaaaaaggct atgaagtaaa taaaaaaatt aagtttgatc gttttaaatt ttataacagt
      301 aaagctcacc ctgatcttac cgttaaagtg agagaaaagg ataacatcgt taaggggata
      361 atattagtaa gagatgaaaa gatacatact aattttgatg ggggaattgg ttcgccgata
      421 aataacgcga ttgaaaatct tggattcgga tataaaagaa caaaagttgg caatgatttc
      481 tcatcggtaa agtatattga tagagataac catttaaaat taaacttact ttatcaagat
      541 ttagaaatta aacgtattga attttttagt aaatagcttt aggtcttaaa gttataaaaa
      601 acgaataaat aattttattg ggatgagtga cctatatgaa caagattact tatttaaatg
      661 aattagaaac agcacttgat gcgctaccta ggcatttaag agatcaaaaa atgtatgaat
      721 atgaacgtta tttttatgag caagaactta atggcgttga tgaaggggaa attttaaaga
      781 agttaaaaga cccacaagat gttgcagctg aaacaaaagc tagaagtgtt attgattatg
      841 ctgaatctaa accaacattt gaaaatattt caagagctgt tgctgcttca ttaagtttag
      901 gcattctatc tatttttgtc atccttatac cagtatctat agttggatta tttgtattag
      961 cattattttt aatatcactt ttgctgctgt tttgtccaat tattttatta gcatcagcaa
     1021 tatccagagg aattgtagac tcaattagta atgtattttt tgccatatca tattca
//
LOCUS       DAHHSC010000355         1066 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.362, whole genome shotgun sequence.
ACCESSION   DAHHSC010000355 DAHHSC010000000
VERSION     DAHHSC010000355.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1066)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1066)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1066
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.362"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            <1..743
                     /locus_tag="NBI57_002712"
     CDS             <1..743
                     /locus_tag="NBI57_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002458658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HCD1823609.1"
                     /translation="NLTVNVPFEELKELSNYYSHDKKLFIQDLEKFYDKVFSLTYREE
                     TENVIRKFILFTKVEIYKDKEYVAIGVNPDLEHIINSLTSNFTKFELREMTHLKSTYS
                     KHMFRILKQYKHTGYVKIKIDDFRERLDIPNSYRMTNINQKVLAPIIKELGFIFNNLN
                     INKIKAKKGRKIEWLEFTFDAEKRIHSKRQPQMVNIGKSRQYISREKTPKWLEERTYE
                     KQTQNEYDPQLEKEREAFLKQLQTDWEE"
BASE COUNT          424 a          143 c          173 g          326 t
ORIGIN      
        1 gcaatttgac tgttaatgtg ccatttgaag aattaaaaga attaagtaat tactatagtc
       61 atgataaaaa gctttttatt caagatttag aaaaattcta tgataaagtt ttctctttaa
      121 catatagaga agaaactgag aatgttatta gaaaatttat tctttttact aaagtagaaa
      181 tttataaaga taaagagtat gtggctattg gtgttaatcc agatttagaa catattatta
      241 attcattaac tagtaacttc actaaatttg aacttagaga aatgacacac cttaaatcta
      301 catattctaa gcatatgttc agaatactta agcagtataa acatactggc tatgtaaaaa
      361 ttaaaattga tgattttaga gaacggctag atattcctaa tagttaccgt atgacaaata
      421 ttaatcaaaa agtattagcc cctatcatta aagaattagg cttcattttt aataatctta
      481 atatcaataa aataaaggca aaaaaaggac gtaaaattga atggttagag tttacttttg
      541 atgctgagaa acgtattcac agtaaacgac agccacaaat ggtgaatata ggtaagtcac
      601 gacaatatat cagtcgtgag aaaacaccaa aatggttaga agaacgcaca tatgaaaaac
      661 aaacacaaaa tgagtatgat ccacaattag agaaagaacg agaagccttt ttaaaacagc
      721 tccaaaccga ttgggaagaa taataaaagt agatgaattt ttacaaatag tatctgctga
      781 tgtatactga aggtaacaag tgaagtatgg ttcgccatat gtcaaaaccc cactgccgac
      841 caaagcaaag tggggttttt tatttatctt tttaggttat ctgtaatgta gttcgcaact
      901 acaccagcaa taacagataa gataaatgtt ccaacaagtg agagtatcgt taacgccata
      961 tgcacacctc ctttgtgcac ggaagtgcct ttctattata aattattaat agaaaggatg
     1021 atatgatgaa acggattaaa ttaaatttct ttgatttaga tgaaat
//
LOCUS       DAHHSC010000356         1062 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.363, whole genome shotgun sequence.
ACCESSION   DAHHSC010000356 DAHHSC010000000
VERSION     DAHHSC010000356.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1062)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1062)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1062
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.363"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            <1..183
                     /locus_tag="NBI57_002713"
     CDS             <1..183
                     /locus_tag="NBI57_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1823610.1"
                     /translation="NKLDSMLVNKINILYSDNEGDGTMTFVTGTEYFKWYFPEYPDKI
                     KTLKIEDVKKHTFTDK"
BASE COUNT          396 a          151 c          175 g          340 t
ORIGIN      
        1 aataaattag attctatgtt agttaataaa ataaacatac tatactcaga taatgaaggt
       61 gatggaacaa tgacatttgt tactggcaca gaatatttta aatggtattt tcctgaatat
      121 ccagacaaaa taaaaacctt gaaaatagaa gatgtaaaaa aacatacgtt tacagataaa
      181 taaatataaa ttatacgctg agaaacgtcc tgtgttgcag taagggataa tattgtgtaa
      241 ctagatatgt ttatcgtaaa tgtggatccg tgaaatatga ctttaaacat cgctggtcaa
      301 tctatctttg cgattggtcg tagattaaaa ccatatgaaa caaaatgact tagtgcatgg
      361 ggaatttagg ctatggcttg aaaattggat taagctaatt atcagctaat aaatttatga
      421 aaatagctga aaatccagaa ttaaatgtcc caccagtgtg atatatgggc gcaagtattt
      481 tatactaata agcaactctt ttcgaaccag aacgcaccaa agaacacata acatcaaacg
      541 gcgaaactaa aatgccgtac taaatgttta tgaatttaac ggaagaactc aattttgagc
      601 cttgtaaaat tacataaatt agttatataa atgctattta tgggtattat ataaacggga
      661 ggggcaacgt tattacttgc ctattagaac atggaatggt tctgccccac ctaatcaggt
      721 attaggtgac ttatggggag aaatcagtta gaatgacata ttcatgtcta tttaagcagg
      781 tgcgttacac acctgctttc tatttacatt taaagataaa atgtgctatt attttactag
      841 aactttttaa catttctctc aagatttaaa tgtagataac aggcaggtac ttcggtactt
      901 gcctattttt ttattcacaa acatctatat caaaaagctt tcatgtgata ataaaaattc
      961 tctcacacgt taaaaacaat gtataagtta attatggctt aacgaaatac attcattcca
     1021 tcgagtagga taggtataag tattgactat tatttttaaa aa
//
LOCUS       DAHHSC010000357         1055 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.364, whole genome shotgun sequence.
ACCESSION   DAHHSC010000357 DAHHSC010000000
VERSION     DAHHSC010000357.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1055)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1055)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1055
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.364"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            complement(90..173)
                     /locus_tag="NBI57_002714"
     tRNA            complement(90..173)
                     /locus_tag="NBI57_002714"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(137..139),aa:Leu,seq:caa)
     gene            complement(212..286)
                     /locus_tag="NBI57_002715"
     tRNA            complement(212..286)
                     /locus_tag="NBI57_002715"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(251..253),aa:Gly,seq:tcc)
     gene            complement(390..464)
                     /locus_tag="NBI57_002716"
     tRNA            complement(390..464)
                     /locus_tag="NBI57_002716"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(429..431),aa:Gly,seq:tcc)
     gene            complement(472..545)
                     /locus_tag="NBI57_002717"
     tRNA            complement(472..545)
                     /locus_tag="NBI57_002717"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(511..513),aa:Cys,seq:gca)
     gene            complement(551..622)
                     /locus_tag="NBI57_002718"
     tRNA            complement(551..622)
                     /locus_tag="NBI57_002718"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(588..590),aa:Gln,seq:ttg)
     gene            complement(634..706)
                     /locus_tag="NBI57_002719"
     tRNA            complement(634..706)
                     /locus_tag="NBI57_002719"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(671..673),aa:His,seq:gtg)
     gene            complement(709..782)
                     /locus_tag="NBI57_002720"
     tRNA            complement(709..782)
                     /locus_tag="NBI57_002720"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(748..750),aa:Trp,seq:cca)
     gene            complement(798..878)
                     /locus_tag="NBI57_002721"
     tRNA            complement(798..878)
                     /locus_tag="NBI57_002721"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(842..844),aa:Tyr,seq:gta)
     gene            complement(884..959)
                     /locus_tag="NBI57_002722"
     tRNA            complement(884..959)
                     /locus_tag="NBI57_002722"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(924..926),aa:Thr,seq:tgt)
     gene            complement(967..1039)
                     /locus_tag="NBI57_002723"
     tRNA            complement(967..1039)
                     /locus_tag="NBI57_002723"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1004..1006),aa:Phe,seq:gaa)
BASE COUNT          314 a          245 c          239 g          257 t
ORIGIN      
        1 ttttatttgt attgtataga gagaaataaa aagaaacctt gttttacaag gtttctaata
       61 cgttatgtta tgtaaataac agttaattat accggtggtc ggggtcgaac cgacactcca
      121 caagtggaac gggattttga gtcccgcgcg tctgccaatt ccgccacacc ggcttaatgg
      181 taaacaaaaa acttcccttt ggaagcaatt atggagcgga agataggatt tacacctata
      241 cctcgttccg ggaaggaacg tgttctaaaa gttgaactac tcccgcaaat attaaattat
      301 ggagcggaag ataggattta cacctatacc tcattccagg aaggaatgta ttctaagagt
      361 tgaaatactc ccgcattatt attaaattat ggagcggaag ataggatttg cacctatacc
      421 tcgttccggg aaggaacgtg ttctaaaagt tgaactactc ccgcataaac ctggaggcgg
      481 caaccggatt tgaaccggtg ataaaggttt tgcagacctc tgccttacca cttggctatg
      541 ccgccaataa ctgggctagc tggattcgaa ccaacgagtg acggagtcaa agtccgttgc
      601 cttaccgctt ggctatagcc cattaataat aagggcggct gaaggggatc gaaccctcga
      661 atgtcggaac cacaatccga tgtgttaacc acttcaccac agccgccatg gcaggggcag
      721 taggaatcga acccacacca aaggttttgg agacctctat tctaccgttg aactatgccc
      781 ctattaaaaa taataaatgg aggggggcag attcgaactg ccgaacccga aggagcggat
      841 ttacagtccg ccgcgtttag ccacttcgct acccctccat aaatggtgcc ggccagagga
      901 cttgaacccc caacctactg attacaagtc agttgctcta ccaattgagc taggccggct
      961 aagaaatggt tcaggacaga gtcgaactgc cgacacatgg agcttcaatc cattgctcta
     1021 ccaactgagc tactgaacca taataaaaat gtaat
//
LOCUS       DAHHSC010000358         1043 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.365, whole genome shotgun sequence.
ACCESSION   DAHHSC010000358 DAHHSC010000000
VERSION     DAHHSC010000358.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1043)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1043)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1043
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.365"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            <1..264
                     /gene="lpl8"
                     /locus_tag="NBI57_002724"
     CDS             <1..264
                     /gene="lpl8"
                     /locus_tag="NBI57_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001797296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein Lpl8"
                     /protein_id="HCD1823611.1"
                     /translation="SINENVPSYDAKFKMSNKDENVKQLRSRYNIPTDKAPVLKMHID
                     GDLKGSSVGYKKLEIDFSKGEKSDLSVIDSLNFQPAKVDEDDE"
     gene            283..>1043
                     /locus_tag="NBI57_002725"
     CDS             283..>1043
                     /locus_tag="NBI57_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein"
                     /protein_id="HCD1823612.1"
                     /translation="MKSIKRIGLCISLLILIIFATSCGSNKITGDSKEEQIKKSFAKS
                     LDKYPTENLEEFYDKEGYRDGEFEKDDKGTWLIRSEMKIQLKGENLESRGAVIEINRN
                     TRTAKGNYIVREVVEDSDGMTHNHTKRYPVKMENNKMIPLKSIDDEKVKKEIEEFKFF
                     VQYGNFKELENYKEDEVSYNPEVPIYSAQYQLKNSDYNVEQLRKRYNIPTQKAPKLLL
                     KGSGNLKGSSVGYKNIEFTFVENKEENIYFTDSVY"
BASE COUNT          433 a          111 c          209 g          290 t
ORIGIN      
        1 tcaattaatg agaatgttcc aagttatgac gcaaaattta aaatgagcaa taaagatgaa
       61 aatgttaagc aattaagaag tcgttataat attcctactg ataaagcacc ggtattaaaa
      121 atgcatattg atggtgattt gaaaggaagt tctgtgggtt ataaaaagtt ggaaattgac
      181 ttttcaaaag gtgaaaaaag cgatttgtca gtaatagatt ctttaaattt ccagccggcg
      241 aaggtggatg aagatgatga atgatgagga tggtgtgtaa caatgaagtc tataaaaagg
      301 attggattgt gcataagtct gttgatttta atcatttttg caacatcttg tggtagtaat
      361 aagattactg gagactcaaa agaagaacaa atcaaaaaga gttttgcgaa atctttagac
      421 aagtatccta ctgaaaatct agaagagttt tatgataaag aaggatatag agatggagaa
      481 tttgaaaaag atgataaagg gacttggcta attagatctg aaatgaaaat ccaattaaaa
      541 ggagaaaatc tggaatctag gggagcagtt atagaaatta acagaaatac tagaacggct
      601 aaagggaatt atattgttag agaagttgtt gaagatagcg atggaatgac acacaatcat
      661 acaaaaaggt atcctgtgaa aatggaaaat aataaaatga ttccattaaa atcaattgat
      721 gatgaaaaag taaaaaaaga aattgaagaa tttaaattct ttgtacaata cgggaatttc
      781 aaagaattgg aaaactataa agaggacgaa gtgtcatata acccagaagt accaatatac
      841 tctgcacaat atcaattgaa aaacagtgat tataatgtag agcaattacg taagcgatat
      901 aatatcccga cgcaaaaagc gcccaaatta ttattgaaag gctcaggtaa tttaaaaggt
      961 tcatcagtcg gatataaaaa tattgaattt acctttgttg aaaataagga agaaaatatt
     1021 tacttcacag atagtgtcta ctt
//
LOCUS       DAHHSC010000359         1020 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.366, whole genome shotgun sequence.
ACCESSION   DAHHSC010000359 DAHHSC010000000
VERSION     DAHHSC010000359.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1020)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1020)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1020
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.366"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            complement(106..903)
                     /locus_tag="NBI57_002726"
     CDS             complement(106..903)
                     /locus_tag="NBI57_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCD1823613.1"
                     /translation="MYHNSNANFVNGITLNVRDKNELKPFYEDVLGLNIINETLTSIQ
                     YEVGQNNHVITLVELQNGREPLMSEAGLFHIAIKLPQISDLANLLIHLSEYDIPVNGG
                     IQPASLSLFFEDPEGNGFKFYVDKDEAQWTRQNDLVKIDIRPLNVPRLVSHATKLLWL
                     GIPDDAIIGALHIKTIHLSEVKEYYLDYFGLEQSAYMDDYSIFLASNGYYQHLAMNDW
                     VSATKRVENFDTYGLAIVDFHYPETTHLNLQGPDGIYYRFNHIEVED"
BASE COUNT          337 a          191 c          131 g          361 t
ORIGIN      
        1 ctttttacat tgcctaagaa cttaatgtcc cagcctcctt cataacttgt ataaaaagat
       61 cattaaaaac gattcattat atggttcgtc cattcaaagt atatactaat cttcaacttc
      121 gatatgatta aagcgataat agataccatc cggaccttgt aaatttaaat gtgttgtttc
      181 aggataatga aagtcaacaa ttgctaatcc atacgtatca aaattttcta cacgtttcgt
      241 tgctgatacc caatcattca tggccaaatg ttgataatag ccattcgatg ctaaaaatat
      301 tgaataatca tccatatatg ccgattgctc taatccaaaa taatcgaggt agtactcttt
      361 tacctctgat aaatgaattg tcttaatatg caatgcacct ataatagcgt catctggaat
      421 acctaaccat aacaattttg ttgcatgact cactaatctc ggtacattta atggtctaat
      481 atcaattttt actaaatcat tttgcctcgt ccattgcgct tcgtctttat caacataaaa
      541 tttaaaaccg tttccttctg ggtcttcaaa aaataatgat aacgaagcag gctgtatacc
      601 tccgttaact ggaatatcat attcgcttaa atgaattagt aaattagcta aatcactaat
      661 ttgaggtagt ttaattgcga tatgaaacag tcccgcttcg gacattaaag gttcacgtcc
      721 attttgtaat tcaacaagtg taatgacatg attattttga cctacttcat attgtatcga
      781 tgttaatgtc tcatttataa tatttaatcc taatacgtcc tcataaaatg gctttaattc
      841 attcttatct ctcacattta aagtgatacc atttacaaag tttgcgttac tgttatgata
      901 cattcaatta actcctatca aattcaatat ctgtgcttac ttttacttaa aattatcgcg
      961 aacataattg agaatagcaa ctataacgca tttataagct tcagagaatt tgtaccattc
//
LOCUS       DAHHSC010000360         1005 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.367, whole genome shotgun sequence.
ACCESSION   DAHHSC010000360 DAHHSC010000000
VERSION     DAHHSC010000360.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1005)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1005)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1005
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.367"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            complement(185..540)
                     /locus_tag="NBI57_002727"
                     /pseudo
     CDS             complement(185..540)
                     /locus_tag="NBI57_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000662030.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
     gene            complement(845..933)
                     /locus_tag="NBI57_002728"
                     /pseudo
     CDS             complement(845..933)
                     /locus_tag="NBI57_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498638.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          315 a          183 c          147 g          360 t
ORIGIN      
        1 aaattagtag agtggtctct ctatttaaaa gatcaaagaa ttaatccaat tatttatatg
       61 gaaacgccaa ctgaagatag aaattaaact tttattttaa atcaccctag ttcataccta
      121 aaagactttc acacaaacaa acaaaggagg aaacttaaat tcctcctttc ccttattact
      181 catactataa ttcaatttta acgtcttcgt ccatttgggc ttcaaattca tctagtaatg
      241 ctcgtgcttc tgcaattgat tgtgtgttca tcaattgatg tcgaagttcg ctagcgctct
      301 tatgccacgc acatagattt taaagaatct acgcaaactc ttgaattgtc gtatttcatc
      361 tttttcatat ttgttaaaca atgataaatg caatctcaac agatctaata gttccttgct
      421 tgtgtgttcg cgtggttctt tttcaaaagc gaatggatta tggaaaatgc cgctaccaat
      481 catgacgcca tcaatgccat atttttctgc aagttcaagt cctgtttttc tatcgggaat
      541 atgggagtgg gacagaaatg atattttccc aaaatttatt tcgtcgtccc actccaactt
      601 gcacattatt gtaagctgac tttccgccag ctactatgtt ggggcccctg actagaattg
      661 aaaaaacttg ttacaagtgc attttcgttc agtcaactac tgccaatata actttgtaga
      721 gcatagaaca ttgatttatg tcccaacctg caatttcgtc acgtaaattt ttaatagctt
      781 cgattaattc ccactgtgca tctactttac taatgtgttt gataaaaact taaataattt
      841 taattcagtc atcagtggca ttaaatcttc tatcattttt agttatagtt gataaattta
      901 tatttataag catatatatg gatatttcat caagaatttt taatcatata aatccgaact
      961 gcaaacatat ttgttgaaat aagaggtatt atttttcggg aaatt
//
LOCUS       DAHHSC010000361          999 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.368, whole genome shotgun sequence.
ACCESSION   DAHHSC010000361 DAHHSC010000000
VERSION     DAHHSC010000361.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 999)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 999)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..999
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.368"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
BASE COUNT          342 a          138 c          160 g          359 t
ORIGIN      
        1 acaaatatct tgtgtttttt caattttaac attttacaca caactgatta gtcaatttaa
       61 acgttgatat gacaatgctt atagcgagtt atacatgaat agataaacgc tttaatgaac
      121 tcccgccgtc tccatatttg tagcctacaa cctttgtgga tgtaggcttt tttattatgt
      181 gtttttcagg ggataatgca ttgcagaatt tgttgtgagt attgatatag cagtgtttgt
      241 ataggtgttt atttgatgga ggaaagagta ataagtgatt atgaattagt ttttgagata
      301 taaggggaca gtgatgtgtg tcaaataagt gtcaaaaaag ttggattctg agttttacat
      361 tcaacattgt tcatgaagaa acttctttat acgcaaaaaa ttctccatgt tatatatgtc
      421 aatataaaaa tgtgaatcgt ctacacttaa ttggataaat ggctactgaa aaagaacttt
      481 tcatttttgt tacgtcacta agtgggtgta gttataaaga gatgagccga gttttgatat
      541 tttcattaga atcaatatgc ctattaacac aatcagcaat agttgacgag acggaaataa
      601 aagaagtcgt agttaagaaa tgcatttcac aacataccat tgtagccatt tttattgttt
      661 tggatgataa actctttttg gaatttttag tttttataat ttgcaactac actacttctt
      721 ttactaatat taatgtctaa gtaatcgata aaaaattctc cattgaataa atgagaagtt
      781 aaaaacttta cttaaccttt ctcattgcat tttcctattc acgattttaa gaacccaaca
      841 tactacaaac gaattttaaa aggcgagagt aaagcttact tgtttattat acagatttaa
      901 aatccaagag tcagaacaga ctactcctct ttataactat aaaaaatagc tatgaaaaaa
      961 tctatcgtca tagattcctt catagctaat cttagtatg
//
LOCUS       DAHHSC010000362          982 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.369, whole genome shotgun sequence.
ACCESSION   DAHHSC010000362 DAHHSC010000000
VERSION     DAHHSC010000362.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 982)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 982)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..982
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.369"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            <1..283
                     /locus_tag="NBI57_002729"
     CDS             <1..283
                     /locus_tag="NBI57_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1433 domain-containing protein"
                     /protein_id="HCD1823614.1"
                     /translation="TLYLNHNTKEPNTIKTVHFTSLKRGPMGDAVIEGYINENKEDDF
                     VAYGSPEHNYQFGGSLIKSKNLSTLLKPVHQTKSPDEIKKELESKKNDR"
     gene            550..960
                     /locus_tag="NBI57_002730"
     CDS             550..960
                     /locus_tag="NBI57_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1433 domain-containing protein"
                     /protein_id="HCD1823615.1"
                     /translation="MSKKKILILTSIMLIILISVASIYFKMKYDEKEKQKSIYYKEQQ
                     ARITLYLKHNTIEPNTIKSVHFTKLETSPMGSAVIEGYINENKEDDFVAYASPENNFQ
                     FVGDLIKSERLSELLKPAHQLKSPDDIKKELNKK"
BASE COUNT          396 a          136 c          145 g          305 t
ORIGIN      
        1 tacactctat cttaatcata atactaaaga acccaatacc atcaaaactg tacatttcac
       61 aagtttaaaa agaggaccta tgggcgatgc agtaattgaa ggttacataa atgaaaataa
      121 agaagatgat tttgttgcat atgggtctcc agaacataat tatcaatttg gtggaagttt
      181 aatcaaaagt aaaaatttaa gcacgttatt aaaaccagta catcaaacca aatcacctga
      241 tgagataaaa aaggaattag agtctaaaaa gaacgaccgc taaaactatg cggccgtttt
      301 aattttgttt tcaagttaat catacttaca tataaaaatg aaaagttgaa tttttagtac
      361 aatttccact gtatttatct gttaaccact ttatacccgc aatatttcgt tgatcttata
      421 taaacacttt tggctgcttt tccttgacct agattaaaat tatagatttt ctttgtaaga
      481 gtgtgttttg tagtatcata aatatgtaaa ctgtataaaa ttccaccaca ttttaggagc
      541 tggtgataga tgtcgaaaaa gaaaatttta atattaacta gtattatgtt aatcatatta
      601 attagtgttg caagtatcta ttttaaaatg aaatatgacg aaaaagaaaa acaaaaatca
      661 atttattata aagaacaaca agcgcgcatt acactttatc ttaagcataa tactatagaa
      721 ccgaacacaa tcaaatctgt acatttcaca aaattggaaa caagtcctat gggaagtgct
      781 gtgattgaag gttacataaa tgaaaataaa gaagatgatt ttgttgccta tgcatcacct
      841 gaaaataact ttcaatttgt aggtgattta attaaaagtg aaagattaag tgagttacta
      901 aaaccagcgc atcaattaaa atcaccagat gatataaaaa aagaactaaa taaaaagtag
      961 agtcactaaa gttaggagtt ac
//
LOCUS       DAHHSC010000363          974 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.370, whole genome shotgun sequence.
ACCESSION   DAHHSC010000363 DAHHSC010000000
VERSION     DAHHSC010000363.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 974)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 974)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..974
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.370"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            complement(<1..>974)
                     /locus_tag="NBI57_002731"
     rRNA            complement(<1..>974)
                     /locus_tag="NBI57_002731"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          228 a          296 c          183 g          267 t
ORIGIN      
        1 cctaatactc agccttactg cttagacgtg caatccaatc gcacgcttcg cctatcctac
       61 tgcgtccccc catcgattaa aacgattata ggtggtacag gaatatcaac ctgttatcca
      121 tcgcctacgc ctgtcggcct cagcttagga cccgactaac ccagagcgga cgagccttcc
      181 tctggaaacc ttagtcaatc ggtggacggg attctcaccc gtctttcgct actcacaccg
      241 gcattctcac ttctaagcgc tccacatgtc cttacgatca tgcttcaacg cccttagaac
      301 gctctcctac cattgtccaa aggacaatcc acagcttcgg taatatgttt agccccggta
      361 cattttcggc gcagtgtcac tcgactagtg agctattacg cactctttaa atgatggctg
      421 cttctaagcc aacatcctag ttgtctgggc aacgccacat ccttttccac ttaacatata
      481 ttttgggacc ttagctggtg gtctgggctg tttccctttc gaacacggac cttatcaccc
      541 atgttctgac tcccaagtta aattaattgg cattcggagt ttgtctgaat tcggtaaccc
      601 gagaggggcc cctcgtccaa acagtgctct acctccaata atcatcactt gaggctagcc
      661 ctaaagctat ttcggagaga accagctatc tccaggttcg attggaattt ctccgctacc
      721 ctcagttcat ccgctcactt ttcaacgtaa gtcggttcgg tcctccattc agtgttacct
      781 gaacttcaac ctgaccaagg gtagatcacc tggtttcggg tctacgacca aatactaaac
      841 gccctattca gactcgcttt cgctacggct ccacatttac tgcttaacct tgcatcaaat
      901 cgtaactcgc cggttcattc tacaaaaggc acgccatcac ccattaacgg gctctgacta
      961 cttgtaagca cacg
//
LOCUS       DAHHSC010000364          974 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.371, whole genome shotgun sequence.
ACCESSION   DAHHSC010000364 DAHHSC010000000
VERSION     DAHHSC010000364.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 974)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 974)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..974
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.371"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            <1..147
                     /locus_tag="NBI57_002732"
     CDS             <1..147
                     /locus_tag="NBI57_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000790884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HCD1823616.1"
                     /translation="EYQRWIDIGIQTINKLLQWKEEQNLQNEEYGFSVSSDKEQHIFS
                     TYHT"
     gene            429..>974
                     /locus_tag="NBI57_002733"
     CDS             429..>974
                     /locus_tag="NBI57_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016170730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1823617.1"
                     /translation="MKKSIYILPLAYIVIESPLQIGNVILTPSLKVIESGHFHTDFSL
                     ISMFNKKEFLSILSTSQLKEDALVIFKDDFFEPTTVDQNYYFINKLSQKADRTLDIIR
                     INENNFGVPEQLPGIPGITSNGWQVAEQLTDHKLRTINGKVMFMIREGIGLYPIDLSS
                     NIYEDKLYNCLYVTSNEINLLF"
BASE COUNT          376 a          137 c          141 g          320 t
ORIGIN      
        1 gagtatcaaa gatggataga tattggtatt caaactataa ataaactttt acaatggaaa
       61 gaagaacaaa atctacaaaa tgaagaatat ggattttcag tctctagtga taaagagcaa
      121 catatctttt caacatacca tacatagtag actaaatgaa gtacatgcgt gtatcgatgc
      181 ctaatgacga attagaggta ttctttaaat gatattcaaa tcttcataag atacacgttc
      241 atagcttcag gcatactcgt gcttcactat tattcgaatc aggtgcaaca acttaaagaa
      301 gtaaaactag attaggtcat agtgatataa agacgactat ggacatttat aaacacatta
      361 caaaatcatc tagagaaaaa ttggcagatg aatttcaaaa acatattaat ttttaggagg
      421 attttataat gaaaaagagt atttatatct taccattagc ttatatagtt attgagtcac
      481 ctttacaaat tggcaatgtt atcttaaccc catctttaaa agtaattgaa tcaggtcatt
      541 ttcatacaga cttttcacta atatcaatgt ttaacaaaaa agagttttta tctattttat
      601 ctacttcgca attaaaagaa gatgcactag ttatttttaa ggacgatttc tttgaaccta
      661 ccacggtaga ccaaaattat tattttatta ataagttaag tcaaaaagcc gacagaactc
      721 tagatattat aagaataaac gaaaataatt ttggtgttcc tgaacaatta ccaggtatac
      781 caggtattac ctcaaatggt tggcaagtag ctgaacaatt gactgaccac aaacttagaa
      841 caattaatgg aaaagtcatg tttatgattc gagaaggaat aggattatat ccaattgact
      901 tatcttctaa tatttatgaa gataaattat ataattgtct ttatgttact tcaaatgaaa
      961 ttaatttact tttc
//
LOCUS       DAHHSC010000365          973 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.372, whole genome shotgun sequence.
ACCESSION   DAHHSC010000365 DAHHSC010000000
VERSION     DAHHSC010000365.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 973)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 973)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..973
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.372"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            698..946
                     /locus_tag="NBI57_002734"
     CDS             698..946
                     /locus_tag="NBI57_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001797059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HCD1823618.1"
                     /translation="MRAFTEANKKSKYQEQTNNARKSEASNCPICNTDLQYDHQTHIW
                     NYKDMVGDHIIPWSKGGKTERGNLQMLYKHHNSLKSNY"
BASE COUNT          421 a          109 c          154 g          289 t
ORIGIN      
        1 attgacattt acatccaact aaatgtagac gattcacaga caaccgatat aattaatttg
       61 tccatccatt ttaggtggca ttttatcatg aggagtatag ttttgattgc aaaaaaacaa
      121 atattagaac atttatcgtg aagaaaaaat gatatacttg aagaaaagta taaatataaa
      181 aagtgaagat atttataaga ggagaaagct aatggctaat acaaaacaaa tccattaaat
      241 caacaagaac ttagaaatgc aatctagtca ggtccattta taacaaaagc aaagtatgaa
      301 ttttctaatt ctatgaatgc taatcagcaa aaaatggcaa agttatatta aaggaattcc
      361 aaatagataa gaaatattag aagaagctct aaatggggga agtaaaggtg atattaatgg
      421 atacatgtca agacatcata atgatataaa tgatttaaaa acatacttta actcagttat
      481 agattggata tcgagcattt ttattaatgt agaaaaagaa atgcggggat tagaatagag
      541 aagtttatat gaaacatatc ataacaactc ttaaaatcct tcagtattag aatataaact
      601 tttaagttta tatgaagata gtgatgtttc aaggaagcta ggaatttttg agtatttatt
      661 aagcggagaa aaattagaat ttataaaata actatcaatt agagctttca ctgaagcgaa
      721 taaaaagtct aaatatcaag agcaaactaa taatgctcga aaaagtgaag cttcaaattg
      781 tcctatttgt aatacagatt tacaatatga tcaccaaaca catatatgga attataaaga
      841 tatggtagga gatcatatta taccatggag taaaggtgga aaaacagagc gtggaaactt
      901 acagatgctt tataaacatc ataatagtct aaaatccaat tactagtaat aaattaatta
      961 tatattatat tcg
//
LOCUS       DAHHSC010000366          962 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.373, whole genome shotgun sequence.
ACCESSION   DAHHSC010000366 DAHHSC010000000
VERSION     DAHHSC010000366.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 962)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 962)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..962
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.373"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            74..>962
                     /locus_tag="NBI57_002735"
     CDS             74..>962
                     /locus_tag="NBI57_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCD1823619.1"
                     /translation="MTDNYSLHIYKSMKMSSVKEANLKIIYWLQGTGVVSINLQQYDV
                     KRNDVTVIMLNDLYQIDGHDDSVCCVVEVSAKTFLKFMNANYMMRGKILTNEVASTFR
                     ILIKYLIYSKIKQQSYNANDKIINYMCVELMSLNTYDGSRHLVAEEVHDYLTNNHHKK
                     INRKDVINQVSITNKALSDMFKATPYSNFIQYLNHIRLEHCLIDILTSKKPIEEIASS
                     HGFNHYSRFIHLFKETYGNTPKLIRKRYSPTSHSINHSQLVEIDKNIIELFDNTNQSS
                     SSMREIDIPFEPTKRSQMYQ"
BASE COUNT          371 a          116 c          158 g          317 t
ORIGIN      
        1 ttagatgttg acaattgatt tgagaaagac aaacaggagt aaatcaatat atttgtatat
       61 gtgaggatgt aagatgacag ataattattc actacatatt tataaaagta tgaaaatgag
      121 tagtgtgaag gaagcgaact taaaaattat atattggcta caaggtacag gtgtagtttc
      181 aatcaatcta caacaatatg atgtaaaaag aaatgatgtc accgttatta tgttgaatga
      241 tttgtatcaa attgatggtc atgatgatag tgtatgttgt gttgttgaag taagtgcaaa
      301 gacatttttg aagtttatga atgcaaacta tatgatgcgt ggcaagatat taacaaatga
      361 ggttgctagc acttttagaa tcttgattaa gtatttaatc tacagcaaaa ttaaacaaca
      421 gtcttataat gctaatgata agattattaa ttacatgtgt gttgaattaa tgtcattaaa
      481 tacatacgat ggtagtcgtc atttagtagc cgaagaagtg cacgattatt taacaaacaa
      541 ccaccataaa aagataaata ggaaagatgt cattaatcaa gttagtatta caaacaaagc
      601 tttaagtgac atgtttaaag cgacacctta tagtaatttt attcaatatt taaatcatat
      661 tagactggag cattgcttaa ttgatatttt aacttctaag aagcctattg aagaaatcgc
      721 aagtagtcat ggttttaatc attattctcg ttttatacac ctctttaaag aaacgtatgg
      781 taacactcct aaattaatta gaaaaagata tagtccaaca tctcattcaa ttaaccattc
      841 ccaattagtt gaaattgata aaaatataat tgaattattt gataatacta atcaaagttc
      901 ttcaagtatg cgcgagattg atataccttt tgaacctaca aaaaggagtc aaatgtatca
      961 ac
//
LOCUS       DAHHSC010000367          934 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.374, whole genome shotgun sequence.
ACCESSION   DAHHSC010000367 DAHHSC010000000
VERSION     DAHHSC010000367.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 934)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 934)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..934
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.374"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            20..874
                     /locus_tag="NBI57_002736"
     CDS             20..874
                     /locus_tag="NBI57_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1823620.1"
                     /translation="MSFYVVLIIIIVALIGILVLNQRYSNSKIDTEVYARKQLIKKNK
                     ALSAENAELRSQMLSSNNDVGHHAYKNAKRELRKILDSYLENGKLKYYDIIVTSNLAT
                     KHPFFEYARSFDFIIVSDIGLINVDVKSWGEKTFYHFDVPDEHDTEMSNSNIEKVVGH
                     YISQQYHDQFNSSRKSIYTFTETVQPNRVIYDFYDYDPYQLAANNAKALKDHIEQNFN
                     FKVQSTGVIYFSDGTVNIIQGSEERDKYVDTVSTKSSLRRIISEAIELSKHPLNKEQV
                     DQITAIFK"
BASE COUNT          357 a          127 c          148 g          302 t
ORIGIN      
        1 aaaaaggatt ggtgaaaaaa tgtcttttta tgttgtactt atcattatca ttgtagcatt
       61 aattggtata ttagttttaa atcaaaggta tagtaatagt aaaatagata ccgaagttta
      121 tgcaagaaag caacttatta aaaagaataa agcattaagt gcagaaaatg ctgaattaag
      181 aagtcaaatg ctgagttcta ataatgatgt tggacaccat gcatacaaaa atgctaaaag
      241 agaattacgc aagattttag atagctatct cgaaaatggt aaattaaaat attacgatat
      301 tatcgttaca agcaatttag ctactaaaca tccgtttttc gagtatgcac gttcatttga
      361 ttttatcatt gtttcagata tcggtttaat aaacgttgat gtaaaaagtt ggggtgaaaa
      421 aacattttat cactttgatg tacctgatga gcatgataca gaaatgagca atagtaatat
      481 tgaaaaagtt gtcggtcatt acattagcca gcaatatcat gatcagttta attcatcaag
      541 aaagtctatc tatactttta cagaaacagt tcaaccaaat cgtgttattt atgattttta
      601 tgattatgat ccatatcaat tagcagcaaa taatgctaaa gcattaaagg atcatattga
      661 acaaaacttt aactttaaag tccaaagtac cggtgttatt tactttagtg atggtacagt
      721 taatatcatt caaggttctg aagaaagaga taaatatgtt gataccgttt caacaaaatc
      781 ttcattacga cgtattatta gcgaagccat agagctatct aaacaccctt taaataaaga
      841 acaggttgat caaatcacag ctatattcaa gtaaaatcaa aggcataata atccattacg
      901 ctcgatacgc actatggtta ttatgccttt aaat
//
LOCUS       DAHHSC010000368          932 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.375, whole genome shotgun sequence.
ACCESSION   DAHHSC010000368 DAHHSC010000000
VERSION     DAHHSC010000368.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 932)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 932)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..932
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.375"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            <1..565
                     /gene="icaB"
                     /locus_tag="NBI57_002737"
     CDS             <1..565
                     /gene="icaB"
                     /locus_tag="NBI57_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="intercellular adhesin biosynthesis
                     polysaccharide N-deacetylase"
                     /protein_id="HCD1823621.1"
                     /translation="YYKKKGKFPKRSVWINFDDMDETIYENAYPILKKYKIPATGFII
                     TGHVGEENFHNLDMISKKELKEMYKTGLWEFETHTHDLHNLSKNNKSKLMKASEATII
                     KDLNKSEKYLTKNFKKSQKTTAYPYGLMNDDKLPVIKKAGLKYGFSLEEKAVTPNSND
                     YYIPRILISDDAFEHLIKRWDGFHEKD"
     gene            552..>932
                     /locus_tag="NBI57_002738"
     CDS             552..>932
                     /locus_tag="NBI57_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HCD1823622.1"
                     /translation="MKKIRLELVYLRAIICAIIIITHLLTQITLKHENMEGGSLVLQF
                     YIRNIVIFGTPCFIILSQLLTTLNYQKVTYRYLTTRVKYILIPYILMGLFYSYSESLL
                     TDSSFNKQFIENVLLGQWYGYFIVV"
BASE COUNT          359 a          133 c          142 g          298 t
ORIGIN      
        1 atattacaag aaaaaaggta agtttccaaa acgaagtgta tggattaact ttgatgatat
       61 ggatgaaact atttatgaaa atgcttatcc aatcttaaaa aaatataaaa taccggcaac
      121 tgggtttatt atcacaggtc atgttgggga agaaaacttt cacaacctcg atatgattag
      181 taaaaaagaa ctaaaagaaa tgtataaaac tgggttatgg gaatttgaaa cacataccca
      241 cgatttgcat aacttatcta aaaataataa gtcaaaatta atgaaagctt ctgaagctac
      301 aatcataaaa gatttaaaca aaagtgaaaa atatctaact aaaaacttta aaaagtcgca
      361 gaaaactaca gcctatcctt atggcttgat gaatgacgat aaattaccgg taatcaaaaa
      421 agctgggtta aaatacggtt tttcattaga ggaaaaagca gtcactccga actccaatga
      481 ttattacatc cctagaatat taattagtga tgatgctttt gagcatttaa ttaagagatg
      541 ggacggattc catgaaaaag attagacttg aactcgtata tttacgtgct attatatgtg
      601 caattattat tatcacacat ttacttacac aaattacttt aaaacatgaa aatatggagg
      661 gaggatcctt agtattacaa ttttacattc gtaatattgt gatttttggt acaccttgct
      721 ttattatctt gtcacagtta ctgacaacct tgaattacca aaaagtcacc tatagatact
      781 taactacacg cgtaaaatat atacttattc cttacatatt aatgggattg ttttacagtt
      841 atagtgaatc attattaaca gattcaagtt tcaataaaca attcattgaa aatgtcctat
      901 taggtcaatg gtatggctat tttatcgttg tt
//
LOCUS       DAHHSC010000369          927 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.376, whole genome shotgun sequence.
ACCESSION   DAHHSC010000369 DAHHSC010000000
VERSION     DAHHSC010000369.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 927)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 927)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..927
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.376"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            <1..593
                     /locus_tag="NBI57_002739"
     CDS             <1..593
                     /locus_tag="NBI57_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCD1823623.1"
                     /translation="FYKEYMYSMTVYSLLSFSLLTFPLDIINEKQNEWRQRLMVTPFT
                     FTSYYISKVVKTMLQFAIAILVIFMVGHFYKGVAMSAVQWLESGIFLWLGASLLITFG
                     ILFSLLNDIQKTSALANIVTIGLAVLGGLWFPINTFPNWLQHVAHVLPSYHLRKLGVD
                     IASNHHINLISFAIILLYVLGSIIAVYCISHFKRAE"
     gene            597..>927
                     /locus_tag="NBI57_002740"
     CDS             597..>927
                     /locus_tag="NBI57_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HCD1823624.1"
                     /translation="MKFLKDTSIAEISSILYLIFPIAGIFFNEVYGPKWLYIISVIVF
                     SLSYLILVIVNNRLNTLMFYILLIIHYFIICYFVFSVHPMLSLFFFYSAFAVPFTFKN
                     NVKKTATN"
BASE COUNT          287 a          107 c          145 g          388 t
ORIGIN      
        1 aattttataa agagtatatg tatagtatga cggtttatag tttgttaagt tttagtttac
       61 taacttttcc attagatatt attaatgaaa aacaaaatga atggcgccaa agattaatgg
      121 taacaccatt tacttttact agttattata tttcaaaagt agtgaaaact atgctgcaat
      181 ttgcaatagc gatattagtt atttttatgg ttggacattt ttataaaggt gttgcaatga
      241 gtgcagttca atggttagag tcaggaatat ttttatggtt aggtgcgtct ctattaataa
      301 cttttggcat attattttct ttgttaaatg atattcaaaa aacaagtgct ttagctaata
      361 tcgtaacaat tggtttagca gtattaggtg gattgtggtt tccgataaac acatttccaa
      421 attggcttca acatgttgct catgttttac cgagctatca tttgcgtaaa ctaggtgtag
      481 atattgcttc aaatcatcat atcaatttaa tatcatttgc tataatactc ttgtatgttt
      541 tagggagtat aatagcagta tattgtatta gtcattttaa aagggcggaa taaaatatga
      601 aatttttaaa agatacttca attgctgaaa tatcgtctat actttatctg atttttccta
      661 ttgccggtat attttttaat gaagtatatg gtcccaaatg gttgtatatt atatcagtca
      721 ttgtcttttc gttgtcgtat cttatattag ttatagtaaa taatagactt aatacattaa
      781 tgttttacat tttgttgatt attcattatt ttattatttg ttattttgtt ttcagtgtac
      841 atccaatgct aagtttgttt ttcttttata gtgcttttgc cgttccattt acttttaaaa
      901 ataatgttaa aaaaacggca actaatc
//
LOCUS       DAHHSC010000370          925 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.377, whole genome shotgun sequence.
ACCESSION   DAHHSC010000370 DAHHSC010000000
VERSION     DAHHSC010000370.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 925)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 925)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..925
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.377"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            complement(6..539)
                     /locus_tag="NBI57_002741"
     CDS             complement(6..539)
                     /locus_tag="NBI57_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HCD1823625.1"
                     /translation="MKSNKSLAMIVVAIIIVGVLAFQFMNHTGPFKKGTNHETVQDLN
                     GKDKVHVQRVVDGDTFIANQNGKEIKVRLIGVDTPETVKPNTPVQPFGKEASNYSKKT
                     LTNQDVYLEYDKEKQDRYGRTLAYVWISKDRMYNKELVEKGLAREKYFSPNGKYRNVF
                     IEAQNKAKQQKLNIWSK"
     gene            678..866
                     /locus_tag="NBI57_002742"
     CDS             678..866
                     /locus_tag="NBI57_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LapA family protein"
                     /protein_id="HCD1823626.1"
                     /translation="MNISNENTVTLIAVIIAIIIGIFLQIFFKLPLIVTAVLSILLGI
                     FVGFIVYLIVSFYNKRKN"
BASE COUNT          285 a          160 c          112 g          368 t
ORIGIN      
        1 tttaattatt tactccaaat atttaatttc tgttgtttag ctttattttg tgcttctata
       61 aatacatttc tatatttgcc atttggtgaa aaatacttct ctctagcaag tcccttttcc
      121 actaattcct tattgtacat acgatcttta cttatccata catacgccaa tgttctacca
      181 tagcgatctt gtttttcttt atcatattct aaataaacat cttgatttgt taatgtcttc
      241 ttactataat ttgatgcttc tttgccaaat ggttgtacag gcgtattcgg tttcaccgtt
      301 tctggcgtat caacccctat aagcctaact ttaatttctt taccattttg atttgcaata
      361 aatgtatcac catccacaac tctttgaaca tgtactttat ctttaccatt taaatcttgt
      421 acagtttcat gattcgtccc ctttttgaaa ggacccgtat gattcataaa ttgaaatgct
      481 aatacaccta caataatgat ggctaccaca atcatagcaa gcgatttatt tgacttcata
      541 ttgccaccta cttctctttt ttcattccga ttaatcatat aaaagttaag aaatatcgtc
      601 aatagccata tctacatatt tcaatatttt ggaaagctat agcatgatat aatgttattg
      661 ttaaggagtg aaaataaatg aacatttcaa atgaaaatac agtcacatta attgcagtaa
      721 ttatcgcaat tatcattggt attttcctac aaatattttt caaattacca ttaatagtga
      781 cggcagtact atctatttta ttgggtatct ttgtaggctt tatcgtttac ttgattgttt
      841 cgttttataa taagcgaaaa aattagttaa caaatctaaa ttgcgagtac caaaataaaa
      901 gtattgagta agattttgat tattg
//
LOCUS       DAHHSC010000371          918 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.378, whole genome shotgun sequence.
ACCESSION   DAHHSC010000371 DAHHSC010000000
VERSION     DAHHSC010000371.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 918)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 918)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..918
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.378"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            complement(239..790)
                     /locus_tag="NBI57_002743"
     CDS             complement(239..790)
                     /locus_tag="NBI57_002743"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HCD1823627.1"
                     /translation="MLTMKDIIRDGHPTLRQKAAELELPLTKEEKETLIAMREFLVNS
                     QDEEIAKRYGLRSGVGLAAPQINISKRMIAVLIPDDGSGKSYDYMLVNPKIVSHSVQE
                     AYLPTGEGCLSVDDNVAGLVHRHNRITIKAKDIEGNDIQLRLKGYPAIVFQHEIDHLN
                     GVMFYDHIDKNHPLQPHTDAVEV"
BASE COUNT          301 a          136 c          147 g          334 t
ORIGIN      
        1 atctaagtat ttcgaataaa tgtgcagttc tatttattaa aataaatgta gagattataa
       61 attaatgtta agaaagaatt aaaggatttt atatgaaacg gtaaaattta aatgagggga
      121 taaaaagttt ggtagaaagg tgaatatatt taactgttgt ttattcgaaa aagttgtaca
      181 agaaaaagct taggacatca tatttatcct aagctttgat aattagtaaa aatgtgcttt
      241 aaacttctac agcatctgta tgtggttgta atgggtgatt tttgtcaatg tgatcataga
      301 acattacacc atttaaatgg tcaatttcat gttggaaaac aattgctgga tatcctttta
      361 gtcgtaattg tatatcatta ccttcgatgt ctttggcttt aattgtaatt ctattatgac
      421 ggtgaactag accagcaaca ttatcatcga cactaaggca accttcacca gttggtaaat
      481 aagcttcttg aacgctatga cttacaattt ttgggttcac aagcatatag tcataagatt
      541 tgccactgcc atcatctggt attaaaacag caatcatacg tttagaaata ttaatttgag
      601 gtgcagccaa accaacgcct gaacgtaaac catatcgttt cgcgatttcc tcatcttgac
      661 tatttactaa aaactctctc atggcgatta atgtttcttt ttcttcttta gttaatggta
      721 attctaactc agctgctttt tgacgcaaag ttggatgacc atctctaatg atgtctttca
      781 ttgttaacat atattgcacc ttccttattt taatttgttt tagttgaatg acagtaaaaa
      841 ggttgttaag atactcatac atttttatgt gtaaatatct acaaagttaa ccaactactg
      901 ccaatgttta ttttagat
//
LOCUS       DAHHSC010000372          914 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.379, whole genome shotgun sequence.
ACCESSION   DAHHSC010000372 DAHHSC010000000
VERSION     DAHHSC010000372.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 914)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 914)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..914
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.379"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            complement(<1..863)
                     /locus_tag="NBI57_002744"
     CDS             complement(<1..863)
                     /locus_tag="NBI57_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001591193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCD1823628.1"
                     /translation="MSYTSVLFNKNFTFYLIGIAFSNMGSAFTTFVFPLMILKLTGSA
                     FQVGIVSALSFIPYAILGLPAGALIDRLNKKTIMKCADIIRFVSYLSIPVLSFFNMLS
                     IFQIYVVAIISGIGLVFHSISEVSIIPSIVKEEDLASANSYIYATQNVSEFLGPIIGG
                     LLYTYMGYSILIFIDSMTFLLSFFSLILIKIETKSIFNQEKLSGKNFLSDVKVGFDYL
                     LSNSTIRVMAVVVSLSNLIISPYYIYIVMFVKEDMNQSSQALGLVLGISSLGALIGSL
                     SASFLLKLFNFG"
BASE COUNT          370 a          155 c          109 g          280 t
ORIGIN      
        1 ccgaaattaa ataatttcaa gagaaaactt gcagataatg aaccgattag cgcacctaat
       61 gatgagatac ctaaaactaa ccccaatgct tgagatgatt gattcatgtc ttcctttaca
      121 aacattacta tataaatata ataaggtgag ataattagat tggataaact tacaacaaca
      181 gccattactc taatagttga attagataat agataatcaa atccaacttt aacatcgctc
      241 aaaaaatttt ttccactcaa cttttcttga ttgaatatac tctttgtttc aattttaatt
      301 aatatcaaac tgaaaaatga gagtaaaaaa gtcatagaat ctataaaaat caatattgaa
      361 tatcccatat atgtataaag taaacctcca attatagggc ccaaaaattc cgaaacattt
      421 tgagtagcgt atatataact gtttgcgctc gccaaatctt cttcttttac aatacttggg
      481 attatcgaaa cctcagaaat actgtgaaat actaatccaa tacctgaaat gattgcaact
      541 acataaattt gaaagatgct taacatattg aaaaaactta atactggtat acttaaatat
      601 gaaacgaacc taattatatc agcacatttc attatggtct tcttattcaa tctgtcaatt
      661 aaagcaccag ctggcaatcc taaaattgca taaggtataa aacttagtgc agaaactatg
      721 ccaacttgaa atgcagatcc agtaagcttt aaaatcatta atggaaaaac aaacgtagta
      781 aaagcactgc ccatgttaga aaaagctatc cctattaaat aaaaagtaaa gttcttatta
      841 aacaatacag aagtatatga cattctatcc ctcttattca tcaaataata aaacgtaatc
      901 tatttatcga aata
//
LOCUS       DAHHSC010000373          908 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.380, whole genome shotgun sequence.
ACCESSION   DAHHSC010000373 DAHHSC010000000
VERSION     DAHHSC010000373.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 908)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 908)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..908
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.380"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            <1..696
                     /gene="sarS"
                     /locus_tag="NBI57_002745"
     CDS             <1..696
                     /gene="sarS"
                     /locus_tag="NBI57_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator SarS"
                     /protein_id="HCD1823629.1"
                     /translation="FRFKKKVKPEVDMTIKEFILLTYLFHQQENTLPFKKIVSDLCYK
                     QSDLVQHIKVLVKHSYISKVRSKIDERNTYISISEEQREKIAERVTLFDQIIKQFNLA
                     DQSESQMIPKDSKEFLNLMMYTMYFKNIIKKHLTLSFVEFTILAIITSQNKNIVLLKD
                     LIETIHHKYPQTVRALNNLKKQGYLIKERSTEDERKILIHMDDAQQDHAEQLLAQVNQ
                     LLADKDHLHLVFE"
BASE COUNT          357 a          145 c          109 g          297 t
ORIGIN      
        1 tttcgtttta agaaaaaagt caagcctgaa gtcgatatga ctataaaaga atttatatta
       61 ctgacttatt tatttcatca gcaagaaaac acacttccat ttaagaagat tgtttcagat
      121 ttatgttata aacaatcgga tttagtacag catataaaag tacttgtgaa acattcatat
      181 attagtaaag ttcgaagtaa aattgatgag cgtaatactt acatttcaat atctgaagaa
      241 caacgagaga aaattgcaga acgtgttaca ttgtttgatc aaatcattaa acaatttaac
      301 cttgcagatc aaagtgaatc acagatgata ccaaaagata gtaaagaatt tctaaacttg
      361 atgatgtata caatgtattt caagaatatt atcaaaaaac atctaacatt aagttttgta
      421 gaattcacaa ttctagctat tatcacttct caaaataaaa acatcgttct tcttaaagat
      481 ttaattgaaa caatccacca taaataccct caaactgtta gagctctcaa taatttaaaa
      541 aagcaaggct atctaataaa agaacgctca actgaagatg aaagaaaaat tttaattcat
      601 atggatgacg cgcagcaaga ccatgctgaa caattattag ctcaagtgaa tcaattatta
      661 gcagataaag atcatttaca tcttgttttt gaataatatc tctattacgc aagtgtgctg
      721 tattctaaag tgcacttgtg ttttctattt tttaataaaa cctcagcaca taatgaacaa
      781 ctttctattt tctatatcac ttaaaaccat ttccgaaatt aaacctcagc acattcaaag
      841 ccccacttta ttcttaaaaa tattttttaa ctcatatgta ttaaaccgct ttcattataa
      901 aaaatatc
//
LOCUS       DAHHSC010000374          892 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.381, whole genome shotgun sequence.
ACCESSION   DAHHSC010000374 DAHHSC010000000
VERSION     DAHHSC010000374.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 892)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 892)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..892
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.381"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            complement(<1..305)
                     /locus_tag="NBI57_002746"
     CDS             complement(<1..305)
                     /locus_tag="NBI57_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1828 domain-containing protein"
                     /protein_id="HCD1823630.1"
                     /translation="MKTIEERMNEYFNWLKQNYIFKELDSSTEITTPFKNHLNDFIRI
                     YADTLPNNEICLSDDGLTINELEMLGIDINTKTRTKLIQNILNQFNLKLVDKEITADV
                     "
     gene            complement(326..562)
                     /locus_tag="NBI57_002747"
     CDS             complement(326..562)
                     /locus_tag="NBI57_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000617704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1823631.1"
                     /translation="MIRFDVNGSDHANPPNNERIPTPHIHIYTEEYNNGGIAIPLKDI
                     EDLELTDEIIESLDFFMKYTNIKHDNVIKEPRLL"
     gene            complement(640..>892)
                     /locus_tag="NBI57_002748"
     CDS             complement(640..>892)
                     /locus_tag="NBI57_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001253430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1823632.1"
                     /translation="NQQQSNKNNYTVIENGNTVTEIYNGQSHTTTNNPIREEYVEGET
                     DPIYKREYKHFYTPEEAQRAQENSDQILREMGLEPERYE"
BASE COUNT          249 a          137 c          119 g          387 t
ORIGIN      
        1 acatccgctg taatttcttt atcaactaat tttaaattaa attgatttaa aatattctgt
       61 ataagtttag ttctcgtttt tgtgtttata tcaataccca acatttctaa ttcattaatc
      121 gtcaatccgt catctgatag acaaatttca ttattaggta gtgtatcagc atatattcta
      181 ataaaatcat tcaaatggtt cttaaaggga gtagtaattt ctgtagaact atctaattct
      241 ttgaagatat aattttgctt taaccagttg aaatattcgt tcattctttc ttcgatagtc
      301 ttcactaagt atccctccat ttcttttaca gtaatcttgg ttcttttata acattatcgt
      361 gttttatatt agtatacttc ataaagaagt ctaatgattc tataatttca tctgttaatt
      421 ctaaatcttc aatatctttt aatgggatcg caataccacc attattatat tcttcagtat
      481 atatgtgtat atgtggcgta ggtattcttt cattatttgg tggatttgca tgatcactac
      541 cattaacatc gaacctaatc atattagaaa tataattatt acttcttagc aaaaaaataa
      601 taagggtagg ggggctaccc gaaattattt agtttgattt tattcatatc tttctggttc
      661 gagacccatt tctcttaaaa tttggtcgct attttcttgt gctctttgag cttcttcagg
      721 tgtataaaaa tgcttatact ctcttttata tatcgggtct gtttctccct ctacatattc
      781 ttctctaata gggttatttg tagttgtgtg tgattgtcca ttataaattt ctgtaactgt
      841 attcccgttt tctataactg tgtaattatt tttattgctt tgttgttgat tg
//
LOCUS       DAHHSC010000375          886 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.382, whole genome shotgun sequence.
ACCESSION   DAHHSC010000375 DAHHSC010000000
VERSION     DAHHSC010000375.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 886)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 886)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..886
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.382"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            25..885
                     /locus_tag="NBI57_002749"
     CDS             25..885
                     /locus_tag="NBI57_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCD1823633.1"
                     /translation="MAKELCFEGITLKAFDEQYRSAINDFDLNERQQIYSSLPKEVID
                     DAINDVDRIANVAINDKNEVVGFFVLHRYYQHEGYDTPENVVYIRSLSINEKYQGFGY
                     GTKIMMSLPQYVQGVFPDFNHLYLVVDAENDNAWNLYERAGFMHTATKEEGPIGKERL
                     YYLDLDSKHVSSLKLEEESRSEVTNVHIINLMIDGQKVGFIALEQIGERMNIAAIEVD
                     KSYRFNGIGSSALRQLPTYLRKNYDNLNVITMILFGENNDFKPLCLNSNFVEIEQTDD
                     YVVFEKYLNY"
BASE COUNT          313 a          116 c          170 g          287 t
ORIGIN      
        1 tataaaaaga taggggttaa atatatggct aaagaacttt gttttgaagg tatcacttta
       61 aaagcatttg atgaacaata tcgttcagca attaatgatt ttgacttgaa tgaaagacaa
      121 caaatatatt catctttacc taaagaagtt attgatgatg caattaatga tgttgatagg
      181 attgctaacg tagcaataaa cgataaaaat gaagtggtgg gcttttttgt attacatcgt
      241 tactatcagc atgaaggtta tgatacacct gaaaatgtcg tttatattcg ttcattatcg
      301 attaatgaaa aatatcaagg ttttggatat ggcacgaaaa taatgatgtc attgccgcaa
      361 tatgttcaag gtgtatttcc tgattttaat catctatatc tagtagtaga tgcggaaaat
      421 gacaatgctt ggaacctata cgaacgtgct ggattcatgc atacagcgac taaagaagaa
      481 ggaccaattg gcaaagaacg actatattac ttggacttag attcaaaaca tgtttcgtca
      541 ttaaagcttg aagaagaaag tcgttcagaa gtgaccaatg tacatatcat taatttaatg
      601 attgatggcc aaaaggttgg ctttatcgca ttggagcaga ttggtgaacg catgaacatt
      661 gctgctattg aagtggataa atcatatcgc tttaatggta ttggttcaag tgcgctgcga
      721 caattgccaa cttacttaag aaaaaactat gacaacctta atgtgattac gatgattctg
      781 tttggagaga ataatgattt taaaccatta tgtttaaata gtaatttcgt tgaaatcgaa
      841 caaactgatg attatgtcgt ttttgaaaaa tatttaaatt actaac
//
LOCUS       DAHHSC010000376          872 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.383, whole genome shotgun sequence.
ACCESSION   DAHHSC010000376 DAHHSC010000000
VERSION     DAHHSC010000376.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 872)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 872)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..872
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.383"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            complement(107..622)
                     /locus_tag="NBI57_002750"
     CDS             complement(107..622)
                     /locus_tag="NBI57_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HCD1823634.1"
                     /translation="MKINTEFKGNNIPYEYAAGADISDLINGNPIKSFPFEITELPKD
                     TKYLAWSLIDYDAIPVCGFAWIHWSVANVSITGDSISIKADLSRTKGDYVQGKNSFTS
                     GLLAEDFSEIENHYVGPTPPDKDHQYELTVYALDHSLNLKNGFYLNEFLKEVNQHKID
                     QTSINLIGRKI"
     gene            complement(600..>872)
                     /locus_tag="NBI57_002751"
     CDS             complement(600..>872)
                     /locus_tag="NBI57_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001610400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1823635.1"
                     /translation="ISMIFLGISSGLTRPQTINKLSSSSNLRVMLNYAETLYFIFNIA
                     FLLIGGYLYSIGTIQYLMLFMSLLTFIYLLTLFYLRRDQHENQHRI"
BASE COUNT          298 a          137 c          110 g          327 t
ORIGIN      
        1 attacttctt tgcattatag cgaacgccgt acctaatata taaattaaat ctcttgtttc
       61 tttgtacttt taattgaaca attttatatt gatgagatat ttagcattaa atttttcttc
      121 ctataaggtt aatacttgtt tgatcaattt tatgttgatt tacttctttt aaaaattcat
      181 tcaagtagaa cccattcttc aaatttaaag aatgatctaa cgcataaact gttaattcat
      241 attgatgatc tttatcaggt ggtgttggtc ctacatagtg attttctatt tctgaaaaat
      301 cttcagccaa caacccacta gtaaagctat ttttaccttg tacatagtca ccctttgttc
      361 ttgataaatc tgcttttata gaaattgaat caccagtgat acttacatta gcaacgctcc
      421 aatgaatcca tgcaaatccg caaactggta ttgcatcata atctatcaga gaccatgcaa
      481 gatatttagt atcttttgga agttctgtta tttcaaacgg gaatgattta attggattgc
      541 catttattaa atcacttata tctgcgcctg ccgcatattc atatggtata ttatttcctt
      601 taaattctgt gttgattttc atgttgatct ctccttaaat aaaataatgt tagtaaatat
      661 ataaacgtca ataatgacat aaataacatt aaatattgaa tagttccaat tgaatataga
      721 taaccaccaa taagtaaaaa tgcgatatta aaaataaaat ataacgtttc tgcataatta
      781 agcatcactc ttaagttact actgctagaa agtttattta tagtttgtgg acgagttaac
      841 cctgaagaaa tacctaaaaa tatcatagaa at
//
LOCUS       DAHHSC010000377          868 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.384, whole genome shotgun sequence.
ACCESSION   DAHHSC010000377 DAHHSC010000000
VERSION     DAHHSC010000377.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 868)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 868)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..868
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.384"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            42..728
                     /locus_tag="NBI57_002752"
     CDS             42..728
                     /locus_tag="NBI57_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HCD1823636.1"
                     /translation="MTEYLLSAGICMAIVSILLIGMAISNVSKGQYAKRFFFFATSCL
                     VLTLVVVSSLSSSANASQTDNGVNRSGSEDPTVYSATSTKKLHKEPATLIKAIDGDTV
                     KLMYKGQPMTFRLLLVDTPETKHPKKGVEKYGPEASAFTKKMVENANKIEVEFDKGQR
                     TDKYGRGLAYIYADGKMVNEALVRQGLAKVAYVYKPNNTHEQLLRKSEAQAKKEKLNI
                     WSEDNADSGQ"
BASE COUNT          337 a          113 c          164 g          254 t
ORIGIN      
        1 gtttaatatt aaaattaact aaaaagaaag aggtgttagt tatgacagaa tacttattaa
       61 gtgctggcat atgtatggca attgtttcaa tattacttat agggatggct atcagtaatg
      121 tttcgaaagg gcaatacgca aagaggtttt tctttttcgc tactagttgc ttagtgttaa
      181 ctttagttgt agtttcaagt ctaagtagct cagcaaatgc atcacaaaca gataacggcg
      241 taaatagaag tggttctgaa gatccaacag tatatagtgc aacttcaact aaaaaattac
      301 ataaagaacc tgcgacatta attaaagcga ttgatggtga tacggttaaa ttaatgtaca
      361 aaggtcaacc aatgacattc agactattat tagttgatac acctgaaaca aagcatccta
      421 aaaaaggtgt agagaaatat ggccctgaag caagtgcatt tacgaaaaaa atggtagaaa
      481 atgcaaataa aattgaagtc gagtttgaca aaggtcaaag aactgataaa tatggacgtg
      541 gcttagcgta tatttatgct gatggaaaaa tggtaaacga agctttagtt cgtcaaggct
      601 tggctaaagt tgcttatgtt tataaaccta acaatacaca tgaacaactt ttaagaaaaa
      661 gtgaagcaca agcgaaaaaa gagaaattaa atatttggag cgaagacaac gctgattcag
      721 gtcaataatg ctcattgtaa aagtgtcact gctgctagtg gcccttttat aatttttaga
      781 tcacgatatg atttattatc aattcagaat taaaaaagta aatagtatca aaagcaagtg
      841 tatttaatat tagaaaataa aaatttta
//
LOCUS       DAHHSC010000378          855 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.385, whole genome shotgun sequence.
ACCESSION   DAHHSC010000378 DAHHSC010000000
VERSION     DAHHSC010000378.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 855)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 855)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..855
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.385"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            <1..468
                     /gene="splD"
                     /locus_tag="NBI57_002753"
     CDS             <1..468
                     /gene="splD"
                     /locus_tag="NBI57_002753"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease SplD"
                     /protein_id="HCD1823637.1"
                     /translation="EIKAHPNGFYNNGGGLYKVTKIVDYPGKEDIAVVQVEEKSTQPK
                     GRKFKDFTSKFNIASEAKENEPISVIGYPNPNGNKLQMYESTGKVLSVNGNIVTSDAV
                     VQPGSSGSPILNSKREAIGVMYASDKPTGESTRSFAVYFSPEIKKFIADNLDK"
     gene            complement(417..524)
                     /locus_tag="NBI57_002754"
     CDS             complement(417..524)
                     /locus_tag="NBI57_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1823638.1"
                     /translation="MLILLLISKNHLSMYGWMIYLSKLSAINFLISGEK"
     gene            626..>855
                     /gene="splF"
                     /locus_tag="NBI57_002755"
     CDS             626..>855
                     /gene="splF"
                     /locus_tag="NBI57_002755"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease SplF"
                     /protein_id="HCD1823639.1"
                     /translation="MNKNIIIKSIAALTILTSVTGVGTTMVEGIQQTAKAENTVKQIT
                     NTNVAPYSGVTWMGAGTGFVVGNHTIITNKHVT"
BASE COUNT          339 a          129 c          142 g          245 t
ORIGIN      
        1 gaaatcaaag cacatcctaa tggtttttat aataacggtg gtggacttta taaagttact
       61 aagattgtag attatcctgg taaagaagat attgcggttg tacaagttga agaaaaatca
      121 acgcaaccaa aaggtagaaa attcaaagat ttcactagca aatttaatat agcatcagaa
      181 gctaaagaaa atgaacctat atcagtcatt ggttatccaa atcctaatgg aaataaacta
      241 caaatgtatg aatcaactgg taaagtacta tcagtgaatg gaaatatagt gacatctgat
      301 gcggttgtcc aacctggcag ctctggttca cctatattaa atagtaagcg agaagcaatt
      361 ggtgttatgt atgctagtga taaaccaaca ggtgaaagta caaggtcatt tgctgtttat
      421 ttctctcctg aaattaagaa atttattgca gataatttag ataaataaat catccatcca
      481 tacattgata aatgattttt agaaattaac aacaaaatca acaattttaa acatctctgt
      541 gattctattt attcgaaatg atttaaaaaa taaaacttca aaaacctaac cttatattta
      601 tacgaatact tagaggagca caaaaatgaa taaaaatata atcatcaaaa gtattgcagc
      661 attgacgatt ttaacatcag tgactggcgt cggcacaaca atggttgaag gtattcaaca
      721 aacagccaaa gccgaaaata ctgttaaaca aattacaaat acaaatgttg caccatacag
      781 tggtgttaca tggatgggcg ctggaacagg atttgtagtt ggaaatcata caatcattac
      841 caataaacat gttac
//
LOCUS       DAHHSC010000379          851 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.386, whole genome shotgun sequence.
ACCESSION   DAHHSC010000379 DAHHSC010000000
VERSION     DAHHSC010000379.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 851)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 851)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..851
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.386"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            <1..389
                     /gene="holA"
                     /locus_tag="NBI57_002756"
     CDS             <1..389
                     /gene="holA"
                     /locus_tag="NBI57_002756"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HCD1823640.1"
                     /translation="QIINRSLEQNVFLLTEYIQKRKKEQAIHLVKDLITMKEEPIKLL
                     ALITSNYRLFYQCKILSQKGYSGQQIAKTIGVHPYRVKLALGQVRHYQLDELLNIVDA
                     CAETDYKLKSSYMDKQLILELFILSL"
     gene            complement(434..685)
                     /locus_tag="NBI57_002757"
     CDS             complement(434..685)
                     /locus_tag="NBI57_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HCD1823641.1"
                     /translation="MANIKSAIKRVKTTEKAEARNISQKSAMRTAVKNAKTAVSNNAD
                     NKNELVSLAVKLVDKAAQSNLIHSNKADRIKSQLMTANK"
BASE COUNT          301 a          112 c          129 g          309 t
ORIGIN      
        1 accaaattat taatagaagt ttagagcaaa atgtattttt actgactgaa tacattcaga
       61 aaagaaagaa agaacaagca attcatttag taaaagattt aataactatg aaagaagaac
      121 caattaaatt acttgcacta attacaagta attaccgatt attttatcaa tgtaagattc
      181 tgagtcaaaa aggatatagt ggacagcaaa ttgctaaaac aataggagtg catccataca
      241 gagtaaaatt agcgttagga caagtaagac attatcaact tgatgaatta ttgaatattg
      301 tagatgcttg tgcggaaact gattataaac ttaaatcatc atatatggat aaacagttaa
      361 tactggaatt attcattcta tctttataaa aaataaaagt tcaagcatgc gcttgaactt
      421 ttatttaaaa agattattta tttgcagtca ttaattgtga tttaatacgg tcagctttgt
      481 ttgaatgtat taaattactt tgagcagctt tgtctactaa cttaactgct aagcttacta
      541 attcattttt attatcagcg ttatttgaaa cagctgtttt agcgttttta actgctgtac
      601 gcattgcact cttttgtgaa atgttgcgtg cttcagcttt ttcagttgtt tttacacgtt
      661 taattgcaga tttgatattt gccatttctg tcacctccta aaagtgagtt catcttatca
      721 aaattttatt tgattacaac aagaaatatt ttaacaaaag tctaaccgtt gtgcaatcat
      781 ttatttgatg gggttagctt aaaattttgc agatttaata aattgtgttg gaaacacaat
      841 caatacgttt t
//
LOCUS       DAHHSC010000380          833 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.387, whole genome shotgun sequence.
ACCESSION   DAHHSC010000380 DAHHSC010000000
VERSION     DAHHSC010000380.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 833)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 833)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..833
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.387"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
BASE COUNT          363 a          149 c           96 g          225 t
ORIGIN      
        1 aatacatagc cattttgaac taacttgcta atcttttatt gatattcaac tatgttataa
       61 aaaaagcaaa ttgaccaaaa tggaagtttg aatcaaaaaa cattaaatgt acccatgact
      121 taaattttat cgccattatt taagtttgga catacacttt tgtcatcaaa cgctgaaaat
      181 tagtacgatt gaaatcactt cttgttataa aaattcttag tagccctgtt acaaaatatt
      241 cgctacaaat tattcaaatc aacaacaata accatcaact aagaaatttt aaacaatcta
      301 ctaaaaaata aagcgtttaa aagttctgtt tagaaaacat caagataagt ataaacatct
      361 agaaaagtga acatctaaga aaatccatct agttattgcc gtaacaatta gaataatcat
      421 ttgaaaaagt gcattagaca acgaaattca ccatcaagat aatgaagcaa caagaaacag
      481 cacacatcaa tgaattaaca ccttagattc atcatgagaa aatgcatggc acaactagat
      541 ttaccatcaa gataattctt aagcagcgaa gaattaacat ctagaaatgc ataaacatct
      601 tagattttac atcaagagat gcgataacta taaacaacta gaaacgtcac caagatagtg
      661 caacaacaaa gaatcagcat cttgaaatcg cactaacatc taagaatcaa catcaagtat
      721 tatattaaca tcatagataa cttcatcaag acaggcatcc acatctaaga aactcatcta
      781 gaatagctaa cacctaacat caaagaatat tcatcaagaa cagtatctaa cag
//
LOCUS       DAHHSC010000381          821 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.388, whole genome shotgun sequence.
ACCESSION   DAHHSC010000381 DAHHSC010000000
VERSION     DAHHSC010000381.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 821)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 821)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..821
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.388"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            98..>821
                     /locus_tag="NBI57_002758"
     CDS             98..>821
                     /locus_tag="NBI57_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1672 family protein"
                     /protein_id="HCD1823642.1"
                     /translation="MLKKAKLILIAALVLSGCSTIENESKKDRNTETNTETKSVPEEM
                     EASKYVGQGFQPPAEKDAIEFAKKHKDKIAKRGEQFFMDNFGLKVKATNVVGSGDGVE
                     VYVHCDDHDIVFNASIPFDKSIIDSDSSLRSEDKGDDMSTLVGTVLSGFEYRAHKEEL
                     DNLTEVLKEYKSKYKYTGYTENAIMKTQNSGFRNEYYYLTAIPYTLDEYKRYFQPLIK
                     EDDKSFRDGMRNSKKQLKDKSRP"
BASE COUNT          333 a          100 c          165 g          223 t
ORIGIN      
        1 atttatatat aggtgaacta aaagatgcaa ataaattaag tgagtataac tatatgcaaa
       61 atgaaactta taaaagcgca taaatgggtg atgagatatg cttaaaaaag caaaattaat
      121 cttaatagca gcactagtac tgtcagggtg ctccacgatt gaaaatgaat caaaaaaaga
      181 cagaaataca gaaacaaata cagaaacgaa atctgtacca gaagaaatgg aagcttcaaa
      241 atatgtaggc caaggattcc aaccgcctgc agaaaaagat gcgattgaat ttgcgaagaa
      301 gcataaagat aaaattgcta aacgtggcga acaatttttt atggataact tcggtctaaa
      361 agttaaagct acgaatgttg taggtagtgg cgacggcgta gaagtatacg tgcattgtga
      421 tgaccacgat atcgtattta atgcgagtat tccatttgac aaatcaatta ttgacagtga
      481 tagctcatta agaagtgagg ataaaggcga tgatatgagt actttagttg gtacagtgtt
      541 gagtggtttt gaatatcgag cacataagga agagttggat aatttaacag aagttttaaa
      601 agaatataaa agtaaatata aatatactgg ttatactgaa aatgcaatta tgaaaacgca
      661 gaatagtggt tttagaaatg agtattatta tttgactgca attccatata ctttagatga
      721 atataaaagg tattttcaac ccttaatcaa agaggatgat aagtctttta gggacggtat
      781 gagaaactca aaaaaacaac ttaaagataa atcacgtcca t
//
LOCUS       DAHHSC010000382          805 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.389, whole genome shotgun sequence.
ACCESSION   DAHHSC010000382 DAHHSC010000000
VERSION     DAHHSC010000382.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 805)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 805)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..805
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.389"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            <1..766
                     /locus_tag="NBI57_002759"
     CDS             <1..766
                     /locus_tag="NBI57_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3427 domain-containing protein"
                     /protein_id="HCD1823643.1"
                     /translation="LVLEELLKNELTYDELKNKMLNEVKDITEDDIDTSLRILDFSFY
                     NAGIEKIYGSPIIECNERMIRLSDAFTNALSNQTFKIFLEDLIELSKYNNEKYQKGKN
                     GLILYNKYSREDFSKIFNWNKNGSSVIMGYMIRSQEMPIFITYDKHEDISDSTKYEDE
                     FLSQDELKWFTKSNRTLKSKEVQKILSHRAKGIKMYIFVQKKDDDGIYFYYLGTAGYI
                     EGSEKQDKMPNGSNVVTMDLALDKAVRDDIYRYITN"
BASE COUNT          353 a           72 c          134 g          246 t
ORIGIN      
        1 tttagtttta gaagaacttt taaaaaatga attaacatat gatgaattaa aaaataaaat
       61 gttgaacgaa gttaaggata taacagaaga tgatatagat acttcattaa gaattttaga
      121 tttttcattt tacaacgcag gaattgaaaa aatctatgga agtcccatta tagaatgtaa
      181 tgaaagaatg ataagactaa gcgatgcatt tactaatgct ctaagtaatc aaacatttaa
      241 aatattttta gaagatctta ttgaattatc aaagtataac aatgaaaaat accaaaaagg
      301 taaaaatgga ctgattttat ataataaata ttcaagagaa gattttagca aaatatttaa
      361 ttggaataaa aatggttcgt ctgtaatcat gggatacatg ataagaagcc aagaaatgcc
      421 aatatttatc acttatgata agcatgagga tataagtgat agtacaaaat atgaagatga
      481 atttttaagt caagatgaat taaaatggtt tactaaatct aatagaacgc taaaatcaaa
      541 agaagttcag aaaattttgt cccatagagc aaaaggaatt aaaatgtata tttttgtaca
      601 gaaaaaagat gatgatggta tatattttta ttatttagga actgctggat atatagaagg
      661 ttcagagaag caagataaaa tgccgaatgg ttcaaacgta gtaacgatgg atttagcact
      721 cgataaagct gtaagagatg atatatatcg ctatataaca aattaatgta aaccgaaaaa
      781 tgaatgttag taaagatgta tttaa
//
LOCUS       DAHHSC010000383          803 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.390, whole genome shotgun sequence.
ACCESSION   DAHHSC010000383 DAHHSC010000000
VERSION     DAHHSC010000383.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 803)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 803)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..803
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.390"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            <1..374
                     /locus_tag="NBI57_002760"
     CDS             <1..374
                     /locus_tag="NBI57_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCD1823644.1"
                     /translation="VNENLDIGLAYKKISKKEKEQLKIRYIEQFGLSNSLKRKVHTLS
                     GGEQQRVALIRMMLKDPIVMLADEPTGALDPKTGQMIIQSLFDLVDENKVLILATHDM
                     AIANQCDEIIDLEQYSKVASM"
     gene            complement(462..752)
                     /locus_tag="NBI57_002761"
     CDS             complement(462..752)
                     /locus_tag="NBI57_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1823645.1"
                     /translation="MFYACHFKMKIGGVILTRTYNIIGILSCLISFIIMALPMIWYTA
                     SALWFFPGAIMILLLSLVIVFCYIKTKNQLHLLLIVLNIIILLFFSLPLLLS"
BASE COUNT          339 a           98 c          132 g          234 t
ORIGIN      
        1 cagtaaatga aaatttagat attggattag catataaaaa aataagtaag aaagaaaaag
       61 aacaattaaa gatacgttat atagaacagt ttggtctgtc aaacagttta aaaagaaaag
      121 ttcatacgct aagtggaggt gaacaacaac gtgtcgcttt aattagaatg atgttaaaag
      181 atccgattgt tatgttagct gatgaaccaa cgggtgcgtt agatcctaaa acaggacaga
      241 tgattattca atcattattt gatttggtcg atgaaaataa agtgctgatt ttagcaacac
      301 atgatatggc tattgcaaat caatgtgacg aaataataga tttagaacag tatagtaaag
      361 tagcatctat gtgatatcca aaagataaaa ttaaaagcca atcaatggga aatgttgact
      421 tcaacactcc gtttgattgg cttttataat gtgttactaa gttatgaaag taacaacggt
      481 aaactgaaaa aaagcaatat aataatattt aatacgatta gtaataaatg taattgattt
      541 ttcgttttaa tatagcaaaa aacaatgact aaagataaca acaatatcat gattgcacct
      601 gggaagaacc ataatgctga agctgtgtac caaatcattg gtaacgccat aataataaaa
      661 gatataagac aagaaaggat accaataata ttataagttc tagtcaaaat tacacctcca
      721 attttcattt taaaatgaca tgcgtaaaac aatcatttta aatacatcat acaataaaat
      781 taaattgtgc ggtagaagaa ttt
//
LOCUS       DAHHSC010000384          802 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.391, whole genome shotgun sequence.
ACCESSION   DAHHSC010000384 DAHHSC010000000
VERSION     DAHHSC010000384.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 802)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 802)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..802
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.391"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            <1..525
                     /locus_tag="NBI57_002762"
     CDS             <1..525
                     /locus_tag="NBI57_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002955256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1672 family protein"
                     /protein_id="HCD1823646.1"
                     /translation="SNDNGDDMSMMVGTVLSGFEYRAQKEKYDNLYKFLKENEKKYQY
                     TGFTKEAINKTQNVGYQNEYFYITYLSRNLKEYRKYYEPLIHKKDKEFKEGMQRARKE
                     LNYTANTDVVSTLFSTKKNFTKDNTVDDVIELSNKLKDKPNMPQKSQVTIQLGKPSIN
                     TKKPFYDDINPIEG"
     gene            537..802
                     /locus_tag="NBI57_002763"
                     /pseudo
     CDS             537..802
                     /locus_tag="NBI57_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829176.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          362 a           88 c          131 g          221 t
ORIGIN      
        1 agtaatgaca acggtgatga tatgagtatg atggtgggta cagtgctgag tggctttgaa
       61 tatcgagcgc aaaaagaaaa gtatgataat ttatataaat ttttaaaaga aaatgaaaag
      121 aaatatcaat atacaggttt tactaaagaa gcaattaaca agacgcaaaa cgtcgggtat
      181 caaaatgaat atttttatat tacatattta tcaagaaatt taaaagaata tcgtaaatat
      241 tacgaaccat tgattcataa aaaggataaa gaatttaaag aaggtatgca acgagctaga
      301 aaagagctaa actatactgc taatactgat gttgtttcta cacttttttc tactaagaaa
      361 aactttacta aagacaatac agtagatgat gtgattgaat tgagtaataa attaaaagat
      421 aaacccaata tgcctcaaaa atctcaagtt actattcaat taggaaaacc aagtataaat
      481 actaaaaagc cgttttatga cgatataaat ccaattgaag gatgagtgaa tataaagtga
      541 ttaaaataaa tgatagagac atagcagaat tgagcagata ttgagtttat caagatataa
      601 atgaaaaaag attttaaagt aaatggaaaa cggttcaaac aaatagatta ttttaatgat
      661 aaaaataacg ataatatgaa agatgcagtt gatataaaaa cctacgagct acttaatgat
      721 cagggagatc caactggtca aaagacaata atatatcaag gaacatctaa agaggcaatt
      781 aatccaaata atccattaaa at
//
LOCUS       DAHHSC010000385          799 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.392, whole genome shotgun sequence.
ACCESSION   DAHHSC010000385 DAHHSC010000000
VERSION     DAHHSC010000385.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 799)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 799)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..799
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.392"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            <1..>799
                     /locus_tag="NBI57_002764"
     CDS             <1..>799
                     /locus_tag="NBI57_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HCD1823647.1"
                     /translation="DLLIDELQSTNEIENVFSTKQEIAHALNNQASEFLKFRGLVDQY
                     KEIELNKKIKVDNVRDIRAIYDKLVSNEINEQDKLDGELFRKNFVGVHDGSTNKYIHV
                     GLQPETKIVEYIGEMLTFLKYFDAPQPFKIMASHYLFEYIHPFYDGNGRVGRFIIAKL
                     LSDYYDNYTALTFSYVINRNKSKYYKAFMTASNHLNCGDLTEFIDTMLELLIAGQERI
                     LDELIPKMDATEKLTLYLTSHYKQIDYEFLYLLSMDKLFGNKRNRLTL"
BASE COUNT          304 a           90 c          141 g          264 t
ORIGIN      
        1 gatttattaa tcgatgaatt acaaagtact aatgaaattg aaaatgtatt tagtactaaa
       61 caagagattg cacatgcgtt aaataaccaa gcatcagaat ttcttaagtt cagaggcctc
      121 gtggatcaat ataaagagat agaacttaat aaaaaaatta aagttgataa tgtaagagac
      181 attagagcga tttatgataa attagtttca aatgaaatta acgaacaaga taagttagat
      241 ggagagctat ttcgtaaaaa ttttgtcggt gtgcatgatg ggtcaacgaa taaatatata
      301 catgttgggt tacaacctga aaccaaaatt gttgaatata taggtgaaat gctaacattt
      361 ttaaaatatt ttgatgcgcc tcagccgttc aaaatcatgg ctagtcatta tctgtttgaa
      421 tatatacatc ctttttatga tggcaatggt agagttggaa gatttataat tgccaagctc
      481 ttgagtgatt attatgataa ttatactgct ttgacatttt catatgttat aaatagaaat
      541 aaatcgaaat attacaaggc gtttatgact gcttcaaatc atttgaattg tggtgatttg
      601 acggaattta ttgacacaat gcttgagcta ttgatagcag gacaagaaag aatacttgat
      661 gagttaatac caaaaatgga tgcgactgaa aaattaacgc tttatttaac tagtcattat
      721 aaacagatag attatgaatt tttgtatctc ctttcaatgg ataaattgtt tggaaataaa
      781 agaaatagat taacattaa
//
LOCUS       DAHHSC010000386          795 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.393, whole genome shotgun sequence.
ACCESSION   DAHHSC010000386 DAHHSC010000000
VERSION     DAHHSC010000386.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 795)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 795)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..795
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.393"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            complement(23..373)
                     /gene="scn"
                     /locus_tag="NBI57_002765"
     CDS             complement(23..373)
                     /gene="scn"
                     /locus_tag="NBI57_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="complement inhibitor SCIN-A"
                     /protein_id="HCD1823648.1"
                     /translation="MKIRKSILAGTLAIVLASPLVTNLDKNEAQASTSLPTSNEYQNE
                     KLANELKSLLDELNVNELATGSLNTYYKRTIKISGLKAMYALKSKDFKKMSEAKYQLQ
                     KIYNEIDEALKSKY"
BASE COUNT          286 a          118 c          101 g          290 t
ORIGIN      
        1 cacccgtaaa agggtggttt ttttaatatt tactttttag tgcttcgtca atttcgttat
       61 aaatcttttg aagttgatat tttgcttctg acattttctt aaagtctttt gacttaagag
      121 catacattgc ttttagacct gaaattttta tagttcgctt ataataagtg tttaaacttc
      181 cagtagctaa ttcattaaca tttagttcat ctaataacga ttttaattca ttagctaact
      241 tttcgttttg atattcattc gatgttggca agcttgtgct agcttgtgcc tcatttttat
      301 ctagattagt tactagtggt gatgctaaaa cgattgctaa agttcccgca agtatagatt
      361 ttctaatttt cattttttat tctccttagt aattagtact aatttatttt aatgactaac
      421 taaaaaaaag tacattaatt attcattaac tatatagatc ttcttattaa atgaaaatta
      481 ataaattatc aaattgacat accttattgt atttatagaa aaacaaaaaa aggtaagcac
      541 cgaaatgctt acctacttcc cataaacaat ataacacata tacattgatt tggaaagcgc
      601 aaaaataaat ttaataaact agcccgaaaa ggagcaatac ataaaaaatg aaaggcgctc
      661 cttgaaaacg cccaaggtaa tattaacata aaatggctac tattgcatta tctaatttta
      721 tttaattaaa acaaatatat agcacaaaaa aactagcccg aaggctagct tatgaataga
      781 tgaaaatttg aacac
//
LOCUS       DAHHSC010000387          793 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.394, whole genome shotgun sequence.
ACCESSION   DAHHSC010000387 DAHHSC010000000
VERSION     DAHHSC010000387.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 793)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 793)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..793
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.394"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            complement(119..226)
                     /locus_tag="NBI57_002766"
     CDS             complement(119..226)
                     /locus_tag="NBI57_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1823649.1"
                     /translation="MVLLAFVTTIHDTDDGRFFNEHKHANNKLLKHSLI"
     gene            complement(263..586)
                     /gene="isdG"
                     /locus_tag="NBI57_002767"
     CDS             complement(263..586)
                     /gene="isdG"
                     /locus_tag="NBI57_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphylobilin-forming heme oxygenase IsdG"
                     /protein_id="HCD1823650.1"
                     /translation="MKFMAENRLTLTKGTAKDIIERFYTRHGIETLEGFDGMFVTQTL
                     EQEDFDEVKILTVWKSKQAFTDWLKSDVFKAAHKHVRSKNEDESSPIINNKVITYDIG
                     YSYMK"
     gene            complement(605..>793)
                     /locus_tag="NBI57_002768"
     CDS             complement(605..>793)
                     /locus_tag="NBI57_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sortase"
                     /protein_id="HCD1823651.1"
                     /translation="RTDFENDQDYQQFLDETKRKSVINSDVNVTVKDKIMTLSTCEDA
                     YSETTKRIVVVAKIIKVS"
BASE COUNT          244 a          147 c           81 g          321 t
ORIGIN      
        1 aaatttcttt tatcgatact actactataa atcatacgcc ccaaaatatc attattaatt
       61 cttttcttct tcaaaataaa tcaaaatgat ataattgatg attattttca aagcacattc
      121 aaatcaaact atgttttagc aatttgttgt tagcatgttt gtgttcatta aaaaaacgac
      181 catcatcggt atcatgtatg gtcgttacaa aagctaacaa taccaattgt cataacaagt
      241 actgcaacct ctttaaattc aattatttca tgtaactata gcctatatca tatgtaatta
      301 ctttgttatt gataatcggg ctactttcat cttcattttt acttctaaca tgtttatgcg
      361 ctgctttaaa gacatcagat tttaaccaat ccgtaaaagc ttgctttgat ttccaaactg
      421 ttaaaatttt cacttcatca aaatcttctt gttctaaagt ttgtgtaaca aacatgccat
      481 caaagccttc taatgtttca atcccatgtc tcgtgtaaaa tcgttctata atatcttttg
      541 ctgttccttt tgttaacgtc agcctatttt ctgccataaa tttcataatt atcctctttt
      601 ctgtttaact taccttaatt atttttgcga caacaacaat tcttttcgtt gtttcactat
      661 atgcatcttc gcacgttgat aaagtcatta ttttatcttt taccgttaca ttaacatctg
      721 aattaattac agatttacgt tttgtctcat ctaaaaattg ttgataatct tgatcatttt
      781 caaaatctgt acg
//
LOCUS       DAHHSC010000388          789 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.395, whole genome shotgun sequence.
ACCESSION   DAHHSC010000388 DAHHSC010000000
VERSION     DAHHSC010000388.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 789)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 789)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..789
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.395"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            complement(161..687)
                     /gene="isaB"
                     /locus_tag="NBI57_002769"
                     /pseudo
     CDS             complement(161..687)
                     /gene="isaB"
                     /locus_tag="NBI57_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501423.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="immunodominant staphylococcal antigen IsaB"
BASE COUNT          247 a          140 c           86 g          316 t
ORIGIN      
        1 tgcttgaaat atgtgagata actatttaaa taatcatgat caaataaaca tttttatcaa
       61 tttcaaacaa ctatgtaaga cgcaataatc gttattattt aataaaaaag actagaatgc
      121 ggtatctctc ctcacactct agccttaata tgccattatt ttatttactt gttttgtatg
      181 gtgtatgtcc aactgttact tcgacaactc tattatgatc aacgacaaac cagatgccta
      241 atgtcttttt agcattttga tagtaaaaaa taccgctatc agcttccttt gttttaccat
      301 tttctttttt caagtccttg ccataagctt tttggatatc ttttaatgaa atattatttt
      361 taactacaaa ttgcaattga cttgcttcgt ttccttttgc agaaacacct ttaaaagttt
      421 gatcatattt ctctactttt ttatcgtatt cgttttagct aatttaatac cattgaattt
      481 cacattatca tacttaatcg cgttaataaa attcttatcc agaataaaat tagcattatt
      541 accaatataa ccattataag tataatatgg ggttattgct gcctgtgcct gtttacttgt
      601 tggcgccgaa ttttcaacaa ctgttactcc aatcagtgtg cccaatgcta atgttgctgc
      661 tacacaaact ttactggttt tattcataat tatctctctt ttccatacaa atattttatt
      721 ccttacactt ttattataac tttttcagcc ataaaaatac taatgattat atttataaat
      781 acataacag
//
LOCUS       DAHHSC010000389          767 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.396, whole genome shotgun sequence.
ACCESSION   DAHHSC010000389 DAHHSC010000000
VERSION     DAHHSC010000389.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 767)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 767)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..767
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.396"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            complement(<1..733)
                     /locus_tag="NBI57_002770"
     CDS             complement(<1..733)
                     /locus_tag="NBI57_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein"
                     /protein_id="HCD1823652.1"
                     /translation="MIHSRKLRLWLYLVLLAVFIGACGMKKEESSKDKQIKENFNKTL
                     SLYPTKNLEDFYDKEGFRDEEFEKGDKGTWIIHSKMTIETNGKNMESRGLVLYVDRNT
                     RTTKGEFIVRELWEDKKGYSRSKEKEYPVKMEHNKIIPTKPIADDKLRKEIEDFKFFV
                     QYGDFKDINDYKDGDISYNPNVPSYSAKYQLSNNDYNVKQLRKRYDIPTKKAPKLLLK
                     GDGDLKGSSIGHKNLEFIFIENKEEN"
BASE COUNT          206 a          149 c           85 g          327 t
ORIGIN      
        1 tgttttcttc tttattttct ataaatataa actctaaatt tttgtgtcct atagatgatc
       61 cttttaaatc accatcacct tttaacaata gtttcggagc tttcttagtt ggtatatcat
      121 acctctttct aagttgcttc acattgtagt cattgttact caattgatat tttgcggaat
      181 aacttggaac attaggatta tatgaaatgt caccatcttt ataatcatta atatctttaa
      241 agtcaccgta ttgtacaaaa aacttaaagt cttcaatttc ttttcttaat ttatcatcag
      301 ctatcggttt agttgggata attttattat gttccatttt cactggatat tctttttctt
      361 tactacgtga gtatcctttt ttatcttccc ataattctct cacaataaat tcaccttttg
      421 tcgttctggt attgcggtct acatacagga ccaagcctct ggattccata ttctttccat
      481 ttgtttctat tgtcatttta gagtgaatta tccaagtccc tttatctcct ttttcaaact
      541 cttcatctcg aaaaccttct ttatcataaa agtcttcaag atttttagtt ggatataagc
      601 tcaacgtttt gttgaagttt tctttaattt gtttatcctt actgctttct tcctttttca
      661 ttccgcaagc gcctataaaa acagctaata atactaaata cagccaaagc ctcaactttc
      721 ttgaatgaat catcatttca catccccatt tttatttttg atgcaaa
//
LOCUS       DAHHSC010000390          749 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.397, whole genome shotgun sequence.
ACCESSION   DAHHSC010000390 DAHHSC010000000
VERSION     DAHHSC010000390.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 749)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 749)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..749
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.397"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            <1..>749
                     /locus_tag="NBI57_002771"
     CDS             <1..>749
                     /locus_tag="NBI57_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1823653.1"
                     /translation="MYFIITLILMTQVYGFFAVIFIIPFTIHIGYIVYKDELNQENVK
                     NHFMWIIVSYGISYLITQIALYGRIDANEIESIDILSVNAFFIIMWLLGQMAIWNFLF
                     LRRALPLTKQELGEEEPELSRTSKGNVTNQTKIHLKQLQDKTTEYARKTRRSVDLDKI
                     RAKRDKFKKKVNDIIDIQEDDIPDWMRKPKWVKPMYVELFCGVVIFLFTFLEFNNRNA
                     LFVSGDWKLSQTQYVIEWVTLLILLFIIIAY"
BASE COUNT          268 a           91 c          129 g          261 t
ORIGIN      
        1 atgtacttta ttattactct tatattaatg actcaagttt atggattctt tgcagtgatt
       61 ttcattattc catttacaat tcatatagga tatatcgtct ataaagatga attgaatcag
      121 gaaaatgtaa aaaatcattt catgtggata attgtgagtt atggtataag ttacttaatt
      181 acacaaattg cattgtatgg cagaattgat gctaatgaaa tagagtcaat tgatatctta
      241 agtgtcaatg ctttctttat aattatgtgg ttacttggtc aaatggctat ttggaatttc
      301 ttgttcttgc gccgagcttt acctttaaca aagcaagaat taggtgaaga ggagccagaa
      361 ttatcaagaa caagtaaagg gaatgtcacg aatcaaacta aaattcactt gaaacaactc
      421 caagataaga ctacagaata tgcacgtaag acaagaagaa gtgtcgattt agataaaatt
      481 agagctaaaa gagataaatt caaaaagaaa gttaatgata ttatcgatat tcaagaagac
      541 gatattcctg attggatgag aaaaccgaaa tgggttaaac caatgtatgt cgaactattt
      601 tgtggtgtcg tcatcttttt attcacattt ttagaattta ataatcgtaa tgcattattt
      661 gtatctggtg attggaaatt atcacagaca caatatgtta ttgaatgggt tacattatta
      721 attctgttat tcattattat cgcatatat
//
LOCUS       DAHHSC010000391          748 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.398, whole genome shotgun sequence.
ACCESSION   DAHHSC010000391 DAHHSC010000000
VERSION     DAHHSC010000391.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 748)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 748)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..748
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.398"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            174..569
                     /gene="spxA"
                     /locus_tag="NBI57_002772"
     CDS             174..569
                     /gene="spxA"
                     /locus_tag="NBI57_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HCD1823654.1"
                     /translation="MVTLFTSPSCTSCRKAKAWLQEHDIPYTERNIFSEHLTIDEIKQ
                     ILKMTEDGTDEIISTRSKTYQKLNVDIDSLPLQDLYSIIQDNPGLLRRPIILDNKRLQ
                     VGYNEDEIRRFLPRKVRTFQLQEAQRMVD"
BASE COUNT          269 a          100 c          110 g          269 t
ORIGIN      
        1 tttataagtg tagaaactta tctgaaaaat taggtttttc tcatttaatt ttaatgttag
       61 atagttataa taaatagtaa cgattaagaa ttttagaaaa aaatgttttt tcttgatcta
      121 taactaaatt ttaagcaatg aattttattc attcaaatag gagagtgaga tgtatggtaa
      181 cattatttac ttcaccaagt tgcacatctt gccgtaaagc gaaagcatgg ttacaagaac
      241 atgacattcc gtatacggag cgtaatattt tttctgaaca tttaacaatt gatgaaatta
      301 agcaaatatt aaaaatgact gaagacggta ctgatgaaat catttctaca cgttctaaaa
      361 cataccaaaa attaaatgtt gatattgatt cactaccatt acaagactta tattcaatca
      421 ttcaagataa tcctggctta ttacgtcgtc caattatttt agataataaa cgactacaag
      481 ttggttataa tgaggacgag attcgacgtt tcttacctag aaaagttcgt acgttccaat
      541 tacaagaagc acaacgtatg gttgactaat aaccatttaa ctatgtgtca tactttacta
      601 aaagatatgt caagcaacac tatttctata gtgttgcttt tttggtttta attttacaat
      661 aattgcttag tatcttttaa atcaaatgtg caattgttaa tatagctcta tattagagtg
      721 ttttggatga aacatttgct atgataag
//
LOCUS       DAHHSC010000392          747 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.399, whole genome shotgun sequence.
ACCESSION   DAHHSC010000392 DAHHSC010000000
VERSION     DAHHSC010000392.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 747)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 747)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..747
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.399"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            <1..>747
                     /locus_tag="NBI57_002773"
     CDS             <1..>747
                     /locus_tag="NBI57_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HCD1823655.1"
                     /translation="TLNNNVVVCTNNDQEVILIGKGIGFNKKEGMALNDQTITIEKIY
                     KLESEQQKAHYKSLVEIADDNVLQVIIDSLNFISNTAMNVDSKQLVVSLTDHIIFAYK
                     RLKQNQVISNPFVMETMQLYSDAYHIAKQVIDQLNAALDVHFPEDEIGFIALHIASNT
                     EDLSMHEMTLINNVIKKGIDIIESDLVTTVDKESLQYQRFIRHVQFLIRRLRRKEYIH
                     AQDDFVSMIKNHYPICYNTAYKILTMIQKQF"
BASE COUNT          292 a           93 c          117 g          245 t
ORIGIN      
        1 acattgaaca acaatgtcgt agtatgtact aataatgatc aagaagttat tttaatcggt
       61 aaaggtattg gttttaacaa aaaagaggga atggcgttaa acgaccaaac tattacaata
      121 gagaaaattt ataaattaga aagtgagcaa caaaaagcac attataaaag tttagttgaa
      181 atcgctgatg ataatgtatt acaagtaatt attgattcgt tgaattttat ttctaatact
      241 gcgatgaatg ttgattcaaa acaacttgta gtttcattaa cggatcatat tatatttgct
      301 tataaacgct taaaacaaaa tcaagttatt agcaatccat ttgttatgga aactatgcag
      361 ttatatagtg atgcatatca tattgctaaa caggtgattg atcagttaaa tgcagcatta
      421 gatgtacatt ttcctgaaga tgagatagga tttattgcat tacatattgc atctaataca
      481 gaagatttat ctatgcatga gatgaccttg atcaataatg ttattaaaaa aggtatagat
      541 atcattgaat cagaccttgt gacaactgtt gataaggaat cattacaata ccaacgtttt
      601 ataaggcacg tacaattttt aattcgccga ttaagaagaa aagaatatat acatgcacaa
      661 gatgattttg tgtctatgat taaaaatcac tatccgattt gctataacac agcatataaa
      721 attttaacta tgatacaaaa acaattt
//
LOCUS       DAHHSC010000393          744 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.400, whole genome shotgun sequence.
ACCESSION   DAHHSC010000393 DAHHSC010000000
VERSION     DAHHSC010000393.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 744)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 744)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..744
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.400"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            <1..326
                     /locus_tag="NBI57_002774"
     CDS             <1..326
                     /locus_tag="NBI57_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001837660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HCD1823656.1"
                     /translation="LMGDASKNNESLLLKKYNLPEIDILKVGHHGSKTSSSKEFIEMI
                     KPKISLISSGKNNMYHLPNIEVVKRLQRIRSRIYNSQQNGQVTIDLDDNLKVDSNSYG
                     NASGL"
     gene            383..>744
                     /locus_tag="NBI57_002775"
     CDS             383..>744
                     /locus_tag="NBI57_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HCD1823657.1"
                     /translation="MSDNIVAIYGDVPELVEKQSAEIISQFLKSDRDDFNFVKYNLYE
                     TEIAPIVEETLTLPFFSDKKAILVKNAYIFTGEKAPKDMAHNVDQLIEFIEKYDGENL
                     IVFEIYQNKLDERKKLTKT"
BASE COUNT          301 a           82 c          134 g          227 t
ORIGIN      
        1 tattaatggg cgatgctagt aaaaataatg aatctttact actaaaaaaa tataacttgc
       61 cggagattga tattttaaaa gtaggacatc atgggagcaa gacaagtagt tctaaagaat
      121 ttatagagat gattaagcct aaaataagtt tgatttcttc tgggaagaac aatatgtatc
      181 atcttcctaa tatagaagtt gttaaacgat tgcaaaggat tcgcagtcgc atttacaata
      241 gtcaacaaaa cggtcaagtt acaattgact tagatgataa tttaaaagtt gattcaaact
      301 cttatggaaa tgcaagtggt ttatagatac agaaacatga tataatctat tgatgtgtca
      361 aacgaatgaa agggtgtaat gaatgagcga caatattgta gctatttatg gagatgtgcc
      421 tgaattggtt gaaaaacaaa gtgcagaaat catatcacaa tttttgaaaa gtgatagaga
      481 tgactttaac tttgtgaaat ataatttata cgaaacagag attgcaccaa ttgttgaaga
      541 aacattaaca ttgcctttct tttcagataa aaaagcaatt ttggttaaaa atgcatatat
      601 atttacaggt gaaaaagcgc caaaagatat ggctcataat gtagatcaat taatagaatt
      661 tattgaaaaa tatgatggcg aaaatttgat tgtctttgag atatatcaaa ataaacttga
      721 tgaaagaaaa aagttaacta aaac
//
LOCUS       DAHHSC010000394          736 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.401, whole genome shotgun sequence.
ACCESSION   DAHHSC010000394 DAHHSC010000000
VERSION     DAHHSC010000394.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 736)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 736)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..736
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.401"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            42..224
                     /locus_tag="NBI57_002776"
     CDS             42..224
                     /locus_tag="NBI57_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000694772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1823658.1"
                     /translation="MKIKNCKIKKETIVYEVLTSGNQPFTYELPKDLSSHNARKYLEF
                     ISQKLDGDKLTKEDSL"
     gene            324..>736
                     /locus_tag="NBI57_002777"
     CDS             324..>736
                     /locus_tag="NBI57_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001558608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HCD1823659.1"
                     /translation="MKKVSVIMPTFNNGEKLHRTISSVLNQTMKSTDYELIIIDDHSN
                     DNGETLNVIKKYKGLVRFKQLKKNSGNASVPRNTGLKMSKAEYVFFLDSDDLLHERAL
                     EDLYNYGKENNSDLIIGKYGVEGKGRSVPKAIFEKG"
BASE COUNT          314 a           80 c          128 g          214 t
ORIGIN      
        1 aaaagacgtc tatttcagca gtgtttgaaa ggaagtttat aatgaaaata aaaaattgca
       61 aaataaaaaa agaaactata gtatatgaag ttttaactag tggtaatcaa ccattcactt
      121 atgagttacc taaagattta tcgtcacata atgcgcgtaa atacttggaa tttatttcac
      181 aaaaattaga tggcgataag ttaaccaaag aagattcatt atgattttac taaacaaaaa
      241 aacgcctact agtgtgaaaa cgtattgatt aatagcgcct atgtggcgct ttaatatata
      301 aagtaagcaa aggagaaatg aaaatgaaaa aagtaagtgt tataatgcca acattcaata
      361 acggcgaaaa attacataga accattagtt ctgtattaaa tcaaacaatg aagagtactg
      421 attacgaatt aattattatt gatgatcatt caaatgacaa tggtgagact ttgaatgtta
      481 taaaaaaata taaaggatta gttcgtttta aacaattaaa aaagaatagt ggaaatgcta
      541 gcgtacctag aaatacaggc ttaaaaatga gcaaagctga atatgtattc tttttagatt
      601 ctgatgattt acttcacgaa agagcactag aagatttata taattacggc aaagaaaata
      661 atagcgattt aataattgga aaatatggag ttgaaggtaa aggaagaagt gttcctaaag
      721 ctatatttga gaaagg
//
LOCUS       DAHHSC010000395          734 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.402, whole genome shotgun sequence.
ACCESSION   DAHHSC010000395 DAHHSC010000000
VERSION     DAHHSC010000395.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 734)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 734)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..734
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.402"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            76..273
                     /locus_tag="NBI57_002778"
     CDS             76..273
                     /locus_tag="NBI57_002778"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1823660.1"
                     /translation="MQVGVGPQQREIGSPIPTDNASWRGPNIEKLDHQFQQTMQVGVG
                     PNTEAGEKSAYKNVQVGGAPT"
     gene            complement(536..721)
                     /locus_tag="NBI57_002779"
     CDS             complement(536..721)
                     /locus_tag="NBI57_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1823661.1"
                     /translation="MNKHINKKATKLLEDYKKVQQRKSEETDRGCLKSVIMLIVLFFL
                     IIFGIVACSTNVNFFFQ"
BASE COUNT          254 a          133 c          140 g          207 t
ORIGIN      
        1 ataagcttat aacaagtttt ttcaatactc gtggggcccc aacacagaga atttcgaaaa
       61 gaaattctac aaacaatgca agttggggtg gggccacaac agagagaaat aggatcacca
      121 attccaacag acaatgcaag ttggcggggc cccaacatag agaaattgga tcaccaattt
      181 caacagacaa tgcaagttgg ggtggggccc aacacagaag ctggcgaaaa gtcagcatac
      241 aaaaatgtgc aagttggcgg ggccccaaca tagaagctgg cgaaaagtca gcatacaaaa
      301 atgtgcaagt tggcggggcc ccaacataga agctggcgaa aagtcagcat acaaaaatgt
      361 gcaagttggg gtaggacatc gataaaaaaa tactttttct ttagaaatta gtatttctta
      421 tgcatgagtt ttactcatgt attcattttt taagtagaca ttagctacag ctaatgataa
      481 agaaccacta cataataaat cattagtggt tctttatcat ttctatctca ctcttttact
      541 ggaagaaaaa gtttacgttt gtagaacatg ccacaatacc aaaaataatt aagaaaaata
      601 agacgataag catgatgaca cttttcaaac aacctctatc agtttcttcc gattttcttt
      661 gttgaacctt tttataatct tcaagtagtt ttgtcgcttt tttatttata tgtttattca
      721 tgatgttgac tcct
//
LOCUS       DAHHSC010000396          731 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.403, whole genome shotgun sequence.
ACCESSION   DAHHSC010000396 DAHHSC010000000
VERSION     DAHHSC010000396.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 731)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 731)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..731
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.403"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            <1..392
                     /locus_tag="NBI57_002780"
     CDS             <1..392
                     /locus_tag="NBI57_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011382158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="stage II sporulation protein M"
                     /protein_id="HCD1823662.1"
                     /translation="IDLGKMIVAQDLNLQEIFFHNLIYFFSAIFGFLTLGLLNIGMLL
                     INSGMLGFFIAHGIYSNQLKSILLVLAPHAIFEILALLIASTFSLGFLSYLYQRMVKK
                     NKIKNKMFHNFVTTIIASLLEVYVTIN"
     gene            404..>731
                     /locus_tag="NBI57_002781"
     CDS             404..>731
                     /locus_tag="NBI57_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020808033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCD1823663.1"
                     /translation="MLVLNNLQKKFTNSKMVLNNVNAKFRSGSINCIVGVNGAGKTTL
                     LNIISSILMPTKGDVYLNSESIFENSTLKKEIFYTPVNPFFYENLSAKDNLYLICSLY
                     NRKIDQI"
BASE COUNT          273 a           82 c          109 g          267 t
ORIGIN      
        1 atattgattt aggtaaaatg atagttgctc aagatttaaa tttacaagag attttctttc
       61 ataatttaat ttatttcttc tctgcaatat ttggttttct gacacttggt ctgttaaata
      121 taggtatgtt actcataaat tctgggatgt taggcttttt tatagcacat ggaatctata
      181 gcaatcaatt aaaatctatt ctattagtat tagcgccaca cgctatattt gagattttag
      241 cacttttaat tgcttcaaca ttctcattag gttttttatc atacctttat caaagaatgg
      301 taaagaaaaa taaaataaaa aataagatgt tccataattt tgtaacaaca ataatcgcat
      361 cattattaga agtttatgta accataaatt aaggaagtgt atcatgctag ttttaaataa
      421 tcttcaaaaa aaatttacta attcaaaaat ggttttgaat aatgtgaatg ctaagtttag
      481 aagtgggagc atcaattgca tagtgggagt aaatggggca ggtaaaacga ctttgttaaa
      541 tattattagt agtatattga tgccaacaaa aggtgatgta tatttaaata gtgaaagtat
      601 ttttgaaaat agcacactaa aaaaagagat tttttacaca ccagtaaatc ccttttttta
      661 tgaaaattta agtgctaaag ataatttgta cttaatatgt agcctatata atcggaaaat
      721 agatcaaata a
//
LOCUS       DAHHSC010000397          715 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.404, whole genome shotgun sequence.
ACCESSION   DAHHSC010000397 DAHHSC010000000
VERSION     DAHHSC010000397.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 715)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 715)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..715
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.404"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            complement(<1..539)
                     /gene="sem"
                     /locus_tag="NBI57_002782"
     CDS             complement(<1..539)
                     /gene="sem"
                     /locus_tag="NBI57_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_058008129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphylococcal enterotoxin type M"
                     /protein_id="HCD1823664.1"
                     /translation="MKRILIIVVLLFCYSQNHIATADVGVLNLRNYYGSYPIEDHQSI
                     NPENNHLSHQLVFSMDNSTVTAEFKNVDDVKKFKNHAVDVYGLSYSGYCLKNKYIYGG
                     VTLAGDYLEKSRRIPINLWVNGEHQTISTDKVSTNKKLVTAQEIDTKLRRYLQEEYNI
                     YGFNDTNKGRNYGNKSKFSS"
BASE COUNT          235 a          115 c           77 g          288 t
ORIGIN      
        1 gaactaaatt ttgacttatt accataattt cttcctttat ttgtatcatt aaagccataa
       61 atattatatt cttcttgtag atatcttctt aatttagtat caatttcttg agctgttact
      121 aactttttat tagttgatac tttgtcagta gatatagttt gatgttctcc attaacccaa
      181 agattaatag gaatacgtct agatttctct aaataatcac ctgctaatgt aactccaccg
      241 tatatatatt tgtttttcaa acaatatcca ctataactta gaccatatac atctacagca
      301 tgatttttga atttctttac atcatcaacg ttcttaaatt cagctgttac tgtcgaatta
      361 tccatagaaa aaactaattg atgcgaaaga tgattatttt caggattaat actttggtgg
      421 tcttcaattg gatagctacc ataatagttc ctaagattca aaactccgac atcagcggtt
      481 gcgatatgat tttgcgaata gcaaaacaat aaaacaacaa tgataagtat tcttttcata
      541 ttttatttct cctatattta aacttctcta tctttattat cttttattgt aaaaataacc
      601 aattcttctt aaatcatcat gattctatcc cacttatata ttattactcg ttaatctggt
      661 tttgttatct atactaaaat agaaagcttt ttatagtaat aaagttagat ttgaa
//
LOCUS       DAHHSC010000398          712 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.405, whole genome shotgun sequence.
ACCESSION   DAHHSC010000398 DAHHSC010000000
VERSION     DAHHSC010000398.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 712)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..712
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.405"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            8..709
                     /gene="pmtD"
                     /locus_tag="NBI57_002783"
     CDS             8..709
                     /gene="pmtD"
                     /locus_tag="NBI57_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011275331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenol-soluble modulin export ABC transporter
                     permease subunit PmtD"
                     /protein_id="HCD1823665.1"
                     /translation="MFKSPLTYLAILVVSSLIATQSILMANSMDNPKHIIVYGSVFAA
                     AKWLLLIIGLMFVVKTITRDFSQGTIQLYMSKVKTRVGYIISKTISIILISILFALIH
                     YVILIVVQASSNGKNLAFSKYVDNLWFFLIFLLFFGLFLFLITLASQKTAMIFSLGVF
                     LVLIVPFIKPFITFIPRYGEKVLDAFDYIPFAYLTDKMISSNFDFSNWQWVISLGSIV
                     IFFILNILYVAKKDI"
BASE COUNT          224 a           93 c          103 g          292 t
ORIGIN      
        1 ttatagtata tttaaaagtc ctttaacata tttagcgata ctagtcgtat ctagtttgat
       61 tgcaactcaa agtatactta tggcaaattc gatggataac ccgaaacata ttattgtcta
      121 tggatctgta tttgctgcag caaaatggtt attgttaata attggattaa tgtttgttgt
      181 taagacaatt acgcgtgatt tttcacaagg tacaattcaa ctatatatga gtaaagttaa
      241 aacacgcgtt ggatacatta tttcgaaaac aatttcaatt attttaattt caatattatt
      301 tgcattaatt cattatgtga ttttgattgt tgtgcaggca tctagtaatg gaaaaaattt
      361 ggcgttttct aaatatgtag ataatttatg gttcttccta atctttttac tattctttgg
      421 cttgttttta ttcttaatca cacttgcatc acaaaaaaca gcaatgatat tttcattagg
      481 tgtattttta gtactcattg taccgtttat taaacctttt attacattta tcccaagata
      541 cggtgaaaaa gttttagatg cttttgatta tatccctttt gcttacttaa ctgataaaat
      601 gattagctct aactttgatt ttagcaattg gcaatgggta atttcattag gttctatagt
      661 gatattcttc attttgaata tcttatatgt cgctaaaaaa gacatttaat aa
//
LOCUS       DAHHSC010000399          707 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.406, whole genome shotgun sequence.
ACCESSION   DAHHSC010000399 DAHHSC010000000
VERSION     DAHHSC010000399.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 707)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 707)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..707
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.406"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            complement(<1..518)
                     /locus_tag="NBI57_002784"
     CDS             complement(<1..518)
                     /locus_tag="NBI57_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superantigen-like protein"
                     /protein_id="HCD1823666.1"
                     /translation="MKKNIMNKLVLSTALLLLGTTSTQLPKTPISFSSEAKAYNISEN
                     ETNINELIKYYTQPHFSLSGKWLWQKPNGSIHATLQTWVWYSHIQVFGSESWGNINQL
                     RNKYVDIFGTKDEDTVEGYWTYDETFTGGVTPAATSSDKPYRLFLKYSDKQQTIIGGH
                     EFYKGNKPVLTL"
BASE COUNT          229 a          111 c          111 g          256 t
ORIGIN      
        1 tttaaagtta atactggttt atttcctttg taaaattcat gtccaccgat gatagtttgt
       61 tgtttatcac tatattttaa aaatagtcta taaggtttat cagatgaagt agctgctggc
      121 gtaacaccac cagtaaatgt ttcatcataa gtccagtaac cttcaactgt gtcctcatct
      181 ttagttccaa atatatcaac gtatttattt cttaactgat taatgtttcc ccaactctcg
      241 gatccaaaca cttgaatatg actataccaa acccacgttt gcaatgttgc atgaatgcta
      301 ccattgggct tttgccatag ccattttcca gataatgaaa aatgcggctg agtgtaatat
      361 ttgattaact cattgatatt agtctcgttt tcactgatat tataggcttt tgcttcagat
      421 gaaaaactga ttggtgtttt aggaagttgt gttgatgtgg ttcctaaaag taacaatgct
      481 gttgataaaa ctaatttatt catgatgttc tttttcatat gaaaatctcc tttgcgtgaa
      541 ttacccaaag tatataagct attacaccga ttcggaatta aataaaagct aaaactatgt
      601 taaataaact taaacagtta gtagtgttat ttaagcaaaa cttatcattt ttaagttttg
      661 gacagaaaca gtacttaata aagtaggcgg gagttatata ttaaaaa
//
LOCUS       DAHHSC010000400          694 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.407, whole genome shotgun sequence.
ACCESSION   DAHHSC010000400 DAHHSC010000000
VERSION     DAHHSC010000400.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 694)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 694)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..694
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.407"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            complement(482..>694)
                     /locus_tag="NBI57_002785"
     CDS             complement(482..>694)
                     /locus_tag="NBI57_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000072626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HCD1823667.1"
                     /translation="KEYIKYATGTSQLVLSQKDLQNIKTKLPSYEEQQKIGDFFSEID
                     RLVEKQSSKVGRLKVRKKELLQKMFV"
BASE COUNT          221 a          101 c           80 g          292 t
ORIGIN      
        1 atccatattt atgtctaaag ataatctcaa tgttgttcac gtcaataaaa ttatccctag
       61 gtttttaaaa attgtacatg tttaaacaat caaaagtgca cattattaaa ttatcatttc
      121 cagttaaact gtcttgatga ttgaatgact cagtattttg gttttgtttt gtctaatttg
      181 agagagttaa tgatgttaga ttatattctc gtataatttc gtttctaggc ttaccatttt
      241 cataaagttt aattatttta atttaaatta tttactaaaa gctcttcagt ctcttgtcac
      301 aataaaatcg cctaacctct tagtttaaca atatctattc ccatagaatt tgtccaacta
      361 agtgtagccg attcactaaa ctccaaaaaa ataaatccca aattataata gtttcaatat
      421 taatatatta caatttattc atagaatttt tatcatttta attaataggc tgttatctgg
      481 attaaacaaa cattttttgt aatagttctt ttttacgtac ttttaatcgt ccgacttttg
      541 aagattgttt ttcaaccaat ctatctattt cactgaaaaa atcaccgatt ttttgttgtt
      601 cttcataaga tggcaattta gtctttatgt tttgcaagtc tttttgtgag agtaccaatt
      661 gtgatgtacc agttgcatat ttaatatatt cttt
//
LOCUS       DAHHSC010000401          687 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.408, whole genome shotgun sequence.
ACCESSION   DAHHSC010000401 DAHHSC010000000
VERSION     DAHHSC010000401.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 687)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 687)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..687
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.408"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            <1..>687
                     /locus_tag="NBI57_002786"
     CDS             <1..>687
                     /locus_tag="NBI57_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tandem-type lipoprotein"
                     /protein_id="HCD1823668.1"
                     /translation="KLMLGICLVLLIILIVGYVIMTKINSRSAQIKDTFNQTLKLYPT
                     KNLEDFYDKEGFRDQEFEKGDKGNWIVDSEMVIELKDKKMESRSMVLYINRNTRTTKG
                     NFIVRELWEDSKGYAQSKDTKYPVKMEHNRIIPTKPIADDKLRKEIENFKFFVQYGDF
                     KDINDYKDGDISYNPNVPSYSAKYQLKNDDYNVKQLRKRYNIPTNKAPKLLLKGDGDL
                     KGSSIGSKNL"
BASE COUNT          285 a           83 c          124 g          195 t
ORIGIN      
        1 agaaattaat gctcggtata tgtttggtat tactcattat attgattgta ggttatgtca
       61 ttatgacaaa aataaatagt cgaagcgccc aaattaaaga cacatttaat caaacattaa
      121 aattatatcc aaccaaaaat cttgaagact tttatgataa ggaaggcttt cgagatcaag
      181 agtttgaaaa gggcgacaaa ggcaattgga ttgttgattc tgagatggta attgagctga
      241 aggataaaaa aatggaatct agaagtatgg tgctctatat aaatcgtaat actagaacaa
      301 cgaaaggtaa ttttattgtt agagaattgt gggaagatag taaagggtat gcacaaagta
      361 aagatacaaa atatccagtg aaaatggaac ataatcgaat tatcccaacg aaaccgatag
      421 ctgatgataa attaagaaaa gaaatagaaa actttaaatt ctttgtacaa tacggtgact
      481 ttaaagatat taatgattat aaagatggtg acatttcata taatcctaat gttccaagtt
      541 attccgcaaa atatcaattg aaaaacgatg attacaatgt gaagcaactt cgaaaaagat
      601 ataatattcc aaccaacaaa gctccgaaac tattattgaa aggtgatggt gatttaaaag
      661 gttcatcaat aggatcaaaa aatctag
//
LOCUS       DAHHSC010000402          685 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.409, whole genome shotgun sequence.
ACCESSION   DAHHSC010000402 DAHHSC010000000
VERSION     DAHHSC010000402.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 685)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 685)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..685
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.409"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            <1..>685
                     /locus_tag="NBI57_002787"
     CDS             <1..>685
                     /locus_tag="NBI57_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001670134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1828 domain-containing protein"
                     /protein_id="HCD1823669.1"
                     /translation="ENETIEFYSPIVDYFGDSISVNISFSGDRYKLTDHGETLWNMEQ
                     LGIDLTSNKKQKKYQLLNNIVQNYSLVIENKHINFYTNRINLSQAIHDYVLAISEISY
                     LGILKKENIKSLFKDEVMQYFLSNRKIYPNIFPEFKVEGKSKLIHSFEAVFPGEATEY
                     VKTIKRIDKNNAKNVLFDWNDVEIHRNKNFDSNARLNIITEQQDDISEAVSTMLSQYN
                     VEVFSFENKK"
BASE COUNT          277 a           88 c          102 g          218 t
ORIGIN      
        1 gaaaatgaaa ctatagaatt ttattcacct atcgtcgatt attttggtga ttctatttct
       61 gtaaatatat ctttttcagg ggatcgatat aaactaactg atcatggtga aactttatgg
      121 aacatggaac aattaggtat agatttaact agtaataaaa aacaaaaaaa gtatcaactt
      181 ctcaataata ttgtacaaaa ctacagtcta gtcatcgaaa ataagcacat aaacttttat
      241 acaaatagaa taaacttatc tcaagctatt catgattatg ttttggctat ttccgagatc
      301 agttatttgg gaatattaaa gaaagaaaat attaagtcct tatttaaaga cgaagtgatg
      361 caatattttc tatcaaacag aaagatttat cctaatatat ttcccgaatt taaggtagag
      421 gggaaatcta agttaatcca tagtttcgaa gcagtatttc caggagaagc tacagagtat
      481 gttaaaacaa ttaaacgaat cgataaaaac aatgcaaaaa atgtcctttt cgattggaat
      541 gatgttgaaa tacatagaaa taaaaatttt gattctaatg ctagacttaa tatcattaca
      601 gaacaacaag atgatattag cgaagcagta tcaacaatgt tgtctcaata taatgtagag
      661 gtattttcat ttgaaaacaa aaaac
//
LOCUS       DAHHSC010000403          678 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.410, whole genome shotgun sequence.
ACCESSION   DAHHSC010000403 DAHHSC010000000
VERSION     DAHHSC010000403.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 678)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 678)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..678
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.410"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            complement(38..>678)
                     /locus_tag="NBI57_002788"
     rRNA            complement(38..>678)
                     /locus_tag="NBI57_002788"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          155 a          197 c          148 g          178 t
ORIGIN      
        1 gtaagtaaaa gtgattttgc ttcgcaaaac atttattttg attaagtctt cgatcgatta
       61 gtattcgtca gctccacatg tcaccatgct tccacctcga acctattaac ctcatcatct
      121 ttgagggatc ttataaccga agttgggaaa tctcatcttg aggggggctt catgcttaga
      181 tgctttcagc acttatcccg tccacacata gctacccagc tatgccgttg gcacgacaac
      241 tggtacacca gaggtatgtc catcccggtc ctctcgtact aaggacagct cctctcaaat
      301 ttcctacgcc cacgacggat agggaccgaa ctgtctcacg acgttctgaa cccagctcgc
      361 gtaccgcttt aatgggcgaa cagcccaacc cttgggaccg actacagccc caggatgcga
      421 tgagccgaca tcgaggtgcc aaacctcccc gtcgatgtga actcttgggg gagataagcc
      481 tgttatcccc ggggtagctt ttatccgttg agcgatggcc cttccatgcg gaaccaccgg
      541 atcactaagt ccgtctttcg accctgctcg acttgtaggt ctcgcagtca agctccctta
      601 tgcctttaca ctctatgaat gatttccaac cattctgagg gaacctttga gcgcctccgt
      661 taccttttag gaggcgac
//
LOCUS       DAHHSC010000404          667 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.412, whole genome shotgun sequence.
ACCESSION   DAHHSC010000404 DAHHSC010000000
VERSION     DAHHSC010000404.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 667)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 667)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..667
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.412"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            10..135
                     /locus_tag="NBI57_002789"
     CDS             10..135
                     /locus_tag="NBI57_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1823670.1"
                     /translation="MERKRKHFKKEFAKSNLSLNNEKWKFVCSKSKHFLKRAVKF"
     gene            complement(276..593)
                     /locus_tag="NBI57_002790"
     CDS             complement(276..593)
                     /locus_tag="NBI57_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5011 domain-containing protein"
                     /protein_id="HCD1823671.1"
                     /translation="MNKLLQSLSALGVSATLVTPNLNADATTNTTPQIKGANDIVIKK
                     GQDYNLLNGISAFDKEDGDLTDKIKVDGQIDTSKSGKYQIKYHVTDSDGAIKISTRYI
                     EVK"
BASE COUNT          224 a           92 c          110 g          241 t
ORIGIN      
        1 ggctagttca ttgaaaggaa gcgcaaacac tttaaaaagg aatttgccaa aagtaattta
       61 tcgttaaaca atgaaaagtg gaaatttgtt tgttcaaaat caaaacattt tttaaaaaga
      121 gcagttaagt tttaataatt tgataggtag tcctttggta tacagaaaaa atacaatatt
      181 taggttaaag ttgcagatga agttggaata acaaataaaa tggtagcaaa acatgttcgt
      241 ttgctaccat tttatttaca gtatagtgat gagggctatt taacctcaat atacctagtg
      301 gaaattttaa ttgcaccatc tgaatcagtg acatgatatt taatttgata tttaccagat
      361 ttagatgtat caatttggcc atcgacttta attttatcgg ttaaatctcc atcttcttta
      421 tcaaatgcac ttatgccgtt tagaaggtta taatcttgac ctttcttaat aacgatatca
      481 ttagcgcctt taatttgtgg tgtagtattc gtcgttgcat ctgcatttaa atttggtgtt
      541 actagtgtag cagaaacacc gagggctgat aatgactgta atagtttatt cataatgtaa
      601 cctccgaatg ataagtcatt tctgagttag ctttactata ccttaattta tattatttac
      661 acatcat
//
LOCUS       DAHHSC010000405          666 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.413, whole genome shotgun sequence.
ACCESSION   DAHHSC010000405 DAHHSC010000000
VERSION     DAHHSC010000405.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 666)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 666)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..666
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.413"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            <1..>666
                     /gene="pmtC"
                     /locus_tag="NBI57_002791"
     CDS             <1..>666
                     /gene="pmtC"
                     /locus_tag="NBI57_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phenol-soluble modulin export ABC transporter
                     ATP-binding protein PmtC"
                     /protein_id="HCD1823672.1"
                     /translation="VDIDNADNIGFLIEHPKLYDNKSGLYNLKLFAQVLGKGFDKAYT
                     DKIIDAFGMRPYIKKKVKKYSMGMKQKLAIAVSLMNKPKFLILDEPTNGMDPDGSIDV
                     LTTIKSLVNELDMRILISSHKLEDIELICDRAVFLRDGHFVQDVNMEVGVASDTTIVT
                     VDHKDFDRTEKYLAEHFQLQNVDKADGHLMINAQKNYQVILKALSELDIYPKYIETRK
                     SSL"
BASE COUNT          258 a           77 c          119 g          212 t
ORIGIN      
        1 aagtagatat tgataatgca gataatatcg gttttttaat tgagcatcct aaattatatg
       61 ataataaatc aggattgtat aacttgaaat tatttgcaca agtattaggt aagggttttg
      121 ataaagcata cacagacaaa attatagatg catttggtat gagaccttat attaaaaaga
      181 aagttaagaa atattcaatg gggatgaagc aaaagttagc aattgcagta tctttaatga
      241 ataaacctaa atttttaatc ttggatgagc ctacaaatgg tatggatcca gatggctcaa
      301 ttgatgtgct gactacaatt aagtctttag taaatgaact tgatatgaga attctaatat
      361 caagtcataa gttagaagat attgaattaa tttgtgatag agctgtattt ttaagagacg
      421 gacattttgt tcaagatgta aacatggagg taggtgttgc atctgacaca acgatagtta
      481 ctgttgatca taaagacttt gatagaactg aaaaatatct tgcagagcat ttccaattac
      541 aaaatgtcga caaagcagac ggacatttaa tgatcaatgc acaaaaaaat tatcaagtta
      601 tactaaaagc attatctgaa ttagatattt atccgaaata tattgaaaca cgtaaaagtt
      661 cattgc
//
LOCUS       DAHHSC010000406          666 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.414, whole genome shotgun sequence.
ACCESSION   DAHHSC010000406 DAHHSC010000000
VERSION     DAHHSC010000406.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 666)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 666)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..666
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.414"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            79..>666
                     /locus_tag="NBI57_002792"
     CDS             79..>666
                     /locus_tag="NBI57_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000411031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-directed DNA polymerase"
                     /protein_id="HCD1823673.1"
                     /translation="MEKIENEIVNKTYLAINSLEELRNMIGIKSDYFYKCLYVNDHFY
                     NVIKIPKRKKDEYRELMIPNMALKNIQRWILDNVLYRRQVHKCATGFVPRKSIVNNAI
                     PHVGQKYILKMDIENFFPSITFKQVRKIFSEMGYKFELATALANLCTVNNQLPQGAPT
                     SPYIANIIFYNIDKRIFSYCQKNNLRYTRYADDITI"
BASE COUNT          262 a           79 c          111 g          214 t
ORIGIN      
        1 taacgactaa ataatgtaat aattaaaaga agatggttta gcaagagttg ctcatttaga
       61 taaaaaatgg agaattaaat ggagaaaatt gagaatgaaa tagttaataa gacatatctt
      121 gcaattaatt ctttggaaga attaagaaat atgattggta ttaaatcaga ctatttttat
      181 aaatgtcttt atgtgaatga ccatttttat aatgttatca aaatacctaa acgaaaaaaa
      241 gatgaatata gagaattgat gattcctaat atggctctca aaaatatcca aaggtggatt
      301 ttagataatg tattgtacag aaggcaagtt cataaatgtg caactggttt tgttcctagg
      361 aaatcaatag tgaacaatgc aatcccacac gttgggcaga aatatatatt gaaaatggat
      421 atagaaaact tttttccgag tatcacattt aagcaggtta gaaaaatatt ttctgagatg
      481 ggctataaat ttgaacttgc gacagcactc gcaaatttgt gtacagtgaa taatcagttg
      541 cctcaaggtg caccaaccag tccatatata gctaatatca tcttttacaa tatagataaa
      601 agaatctttt cttattgtca aaaaaataat ttgagatata ctcgatatgc tgatgatatt
      661 actatt
//
LOCUS       DAHHSC010000407          653 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.415, whole genome shotgun sequence.
ACCESSION   DAHHSC010000407 DAHHSC010000000
VERSION     DAHHSC010000407.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 653)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 653)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..653
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.415"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            <1..>653
                     /locus_tag="NBI57_002793"
     CDS             <1..>653
                     /locus_tag="NBI57_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000803170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC family ATPase"
                     /protein_id="HCD1823674.1"
                     /translation="KTGSGKTMIFDAMTYALFGKASTEQREENDLRSHFADGKQPMSV
                     TFEFQLNHRIYKVHRQGPYIKEGNTTKTNAKFDVFEMVDGKYEIRESKVISGTQFIIE
                     LLGVNADQFRQLFILPQGEFKRFLISNSREKQGILRTLFDSEKFEAIREILKEELKKE
                     KAQIENRYQQIDLLWQEIESFDDDKIKGLLELATQQIDKLIENIPLLQARSKEILAFV
                     "
BASE COUNT          264 a           86 c          122 g          181 t
ORIGIN      
        1 aagactggat cgggtaaaac aatgattttt gatgcaatga cttatgcctt gtttggtaaa
       61 gcatcaactg aacaaagaga agaaaatgat ttgagaagtc atttcgctga tggtaaacag
      121 ccgatgtcag taacatttga atttcaatta aatcatcgaa tttataaagt gcatagacaa
      181 ggcccttata tcaaagaagg taatacaaca aaaacgaacg ctaaatttga tgtatttgag
      241 atggtggatg gcaagtatga aattagagaa agtaaagtaa tttcaggtac ccaattcatt
      301 attgaattat taggagtaaa tgcagatcaa ttccgacaat tgtttatttt gcctcaaggt
      361 gaattcaaac gctttttaat atcaaacagt cgtgaaaagc aagggatatt aagaacactg
      421 tttgacagtg aaaaatttga agctatacga gaaatattaa aagaagaact aaaaaaagaa
      481 aaagctcaaa tcgagaatag atatcaacaa attgaccttt tatggcaaga aattgaatca
      541 tttgatgatg acaaaataaa aggcttatta gagcttgcca ctcagcagat agacaaattg
      601 attgaaaata taccactttt acaagctagg tcgaaagaaa tactagcatt tgt
//
LOCUS       DAHHSC010000408          652 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.416, whole genome shotgun sequence.
ACCESSION   DAHHSC010000408 DAHHSC010000000
VERSION     DAHHSC010000408.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 652)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 652)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..652
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.416"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            <1..333
                     /locus_tag="NBI57_002794"
     CDS             <1..333
                     /locus_tag="NBI57_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HCD1823675.1"
                     /translation="GFQKIHMKISEINKIEVESVIPILKDKNVVFTGNFDTEKQDLMI
                     LTRKKGAYIRSDVTAKTDILVEGVQDDKYKDVNGLVSKQRKAREYVGNGAKIQFLNEE
                     DLINLIKE"
     gene            339..>652
                     /locus_tag="NBI57_002795"
     CDS             339..>652
                     /locus_tag="NBI57_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1823676.1"
                     /translation="MIKKIFTKKHVFLVIEDENHNHSDAVFGKSILLSIYVGVNKKTN
                     SKSGKFIYLDRSKRIVRQSDITKIESANENDVDFYNLLKKEKEIVYSKNIVDKYNLAN
                     YI"
BASE COUNT          290 a           66 c          104 g          192 t
ORIGIN      
        1 ggatttcaaa aaattcatat gaaaatatct gagattaata aaatagaagt ggaaagtgta
       61 atccctattt taaaagataa aaatgttgtt tttacaggta attttgacac tgaaaaacaa
      121 gatttaatga tattaactag aaagaaagga gcttatatca gaagtgacgt aactgcaaaa
      181 acagatattt tagtcgaagg tgttcaagat gataaatata aagatgtgaa cggactagtt
      241 tcaaaacaac gaaaagctcg agaatatgtt ggaaatggtg caaaaattca atttttaaat
      301 gaagaagact taataaattt aataaaggaa taataacgat gatcaaaaaa atttttacaa
      361 aaaaacatgt attcttagtt atagaagatg aaaaccataa tcacagcgat gctgtttttg
      421 gaaaaagtat attactttca atttacgtcg gtgtgaataa aaagactaat tctaaatcag
      481 ggaaatttat ataccttgac agatctaaaa gaatcgttag acaatctgat atcaccaaaa
      541 tagaatcagc taacgaaaat gatgtagatt tttataattt actgaagaaa gaaaaggaaa
      601 ttgtttattc caaaaatata gtagataaat acaatttagc gaactatata at
//
LOCUS       DAHHSC010000409          650 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.417, whole genome shotgun sequence.
ACCESSION   DAHHSC010000409 DAHHSC010000000
VERSION     DAHHSC010000409.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 650)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 650)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..650
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.417"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            <1..>650
                     /locus_tag="NBI57_002796"
     CDS             <1..>650
                     /locus_tag="NBI57_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001652331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HCD1823677.1"
                     /translation="KFSPATYTIIFVNVLIWLCMILYLNNFSDVKLLDVGGLVHFNVV
                     HGEWYRIVTSMFLHFSFEHILMNMLSLFIFGKIVEAIIGSWRMLTVYFIAGLFGNFVS
                     LSFNTTTISVGASGAIFGLIGSIFAMMYVSKTFNKKMLGQLLIALVILVGVSLFMSNI
                     NIVAHIGGFIGGLLITLIGYYYKVNRNIFWILLIGMLVIFIALQIRIFTIKEDNIY"
BASE COUNT          198 a           62 c          116 g          274 t
ORIGIN      
        1 aaattttcac cggcaacata cacaataata tttgtaaatg tcttaatatg gttatgtatg
       61 attttatatt taaataattt ttcggatgta aaattattag atgttggcgg gttagtgcat
      121 tttaatgtcg tacatggtga atggtatcga attgttacat cgatgttttt acattttagt
      181 tttgaacata tacttatgaa tatgctttca ttatttattt ttggtaaaat agtcgaagca
      241 attattggtt catggcggat gttaactgta tactttattg cagggttgtt tggaaacttt
      301 gtatcactat catttaatac gactacaatt tcagttgggg ctagtggtgc tatatttggt
      361 ctgattggat caatttttgc gatgatgtat gtttcaaaaa catttaacaa aaaaatgtta
      421 ggacagttat taattgcatt agtgatatta gttggtgttt ctctgtttat gtcaaatata
      481 aatattgtgg cgcatattgg aggattcatt ggtggtttat taataacttt aattggctat
      541 tactataaag tgaatcgtaa tattttttgg attttactaa ttggtatgct tgttatattt
      601 attgcacttc aaattagaat ttttacaatt aaagaagata atatttataa
//
LOCUS       DAHHSC010000410          643 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.418, whole genome shotgun sequence.
ACCESSION   DAHHSC010000410 DAHHSC010000000
VERSION     DAHHSC010000410.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 643)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 643)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..643
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.418"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            complement(<1..>643)
                     /locus_tag="NBI57_002797"
     CDS             complement(<1..>643)
                     /locus_tag="NBI57_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HCD1823678.1"
                     /translation="LPESYNQLVKYNVILCFDGLDFLRFGRIQRTYESLIKEARIDDA
                     IIVGFHYEDVDKRREEFHPQGSRSHLTIQSVGKEILPFIDSTFSTLKVGNARLLVGDS
                     LAGSIALLTALTYPTIFSRVAMLSPHSDDKVLDKLNQCANKEQLTIWHVIGLDEKDFT
                     LPTNGKRADFLTPNRELAEQIKKYNITYYYDEFEGGHQWKDWKPLLSDILLYF"
BASE COUNT          212 a          124 c           91 g          216 t
ORIGIN      
        1 aaaaaataca ataatatatc tgacagcaat ggtttccaat ctttccattg gtgaccacct
       61 tcaaattcat cgtaataata agttatatta tatttcttaa tttgttcagc taattctcta
      121 ttcggtgtta agaaatcggc acgcttacca tttgttggta aagtaaaatc tttttcatct
      181 agaccaatga catgccaaat tgtcaattgt tctttattag cacattgatt tagcttatct
      241 aatactttgt catctgaatg tggacttaac attgctacac gactaaaaat cgttggatag
      301 gtcaacgccg ttaataaggc aatactaccc gctaaactat cccctactaa taaccttgca
      361 ttacctactt tcagtgtaga aaacgtcgag tcaataaatg gcaatatttc tttaccgact
      421 gattgaatag ttaaatgaga acgacttcct tgtggatgaa attcctctct acgcttatca
      481 acgtcttcat aatggaatcc aacaatgatc gcatcatcaa tacgcgcttc tttgattaac
      541 gattcatatg tacgttgtat tctcccgaaa cgtaaaaaat ctaatccgtc aaagcaaaga
      601 atgacattat atttaacaag ttgattataa gattctggta aat
//
LOCUS       DAHHSC010000411          628 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.419, whole genome shotgun sequence.
ACCESSION   DAHHSC010000411 DAHHSC010000000
VERSION     DAHHSC010000411.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 628)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 628)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..628
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.419"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            <1..158
                     /locus_tag="NBI57_002798"
     CDS             <1..158
                     /locus_tag="NBI57_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_058008129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="exotoxin"
                     /protein_id="HCD1823679.1"
                     /translation="FHLNDGSSFSYDLFDTGTGQAESFLKIYNDNKTVETEKFHLDVE
                     ISYKDES"
     gene            193..>628
                     /gene="sei"
                     /locus_tag="NBI57_002799"
     CDS             193..>628
                     /gene="sei"
                     /locus_tag="NBI57_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_031927819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphylococcal enterotoxin type I"
                     /protein_id="HCD1823680.1"
                     /translation="MKKFKYSFILVFILLFNIKDLTYAQGDIGVGNLRNFYTKHDYID
                     LKGVTDKNLPIANQLEFSTGTNDLISESNNWDEISKFKGKKLDIFGIDYNGPCKSKYM
                     YGGATLSGQYLNSARKIPINLWVNGKHKTISTDKIATNKKLVT"
BASE COUNT          249 a           81 c           94 g          204 t
ORIGIN      
        1 tatttcattt gaatgatggt tcatcatttt cttatgactt atttgatact ggaacaggac
       61 aagctgaaag tttcttaaaa atatataatg acaacaaaac tgtcgaaact gaaaaattcc
      121 atttagatgt agaaatatct tataaggacg aaagttgaag tatttcaatc ataacttagt
      181 aaaggaaatg ccatgaaaaa atttaaatat agttttatat tagtttttat attacttttt
      241 aacattaaag atcttacgta tgctcaaggt gatattggtg taggtaactt aagaaatttc
      301 tatacaaaac atgattatat agatttaaaa ggcgtcacag ataaaaacct acctattgca
      361 aatcaactcg aattttcaac aggtaccaat gatttgatct cagaatctaa taattgggac
      421 gaaataagta aatttaaagg aaagaaactg gatatttttg gcattgatta taatggtcct
      481 tgtaaatcta aatacatgta tggaggggcc actttatcag gacaatactt aaattctgct
      541 agaaaaatcc ctattaatct ttgggttaat ggcaaacata aaacaatttc tactgacaaa
      601 atagcaacta ataaaaaact agtaacag
//
LOCUS       DAHHSC010000412          622 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.420, whole genome shotgun sequence.
ACCESSION   DAHHSC010000412 DAHHSC010000000
VERSION     DAHHSC010000412.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 622)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 622)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..622
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.420"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            complement(304..504)
                     /locus_tag="NBI57_002800"
     CDS             complement(304..504)
                     /locus_tag="NBI57_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HCD1823681.1"
                     /translation="MNNGTVKWFNAEKGFGFIEREDGSDVFVHFSAIAEDGYKSLEEG
                     QKVEFDIVEGDRGEQAANVVKM"
BASE COUNT          198 a          118 c           79 g          227 t
ORIGIN      
        1 attattgtta aacattttga gtgtttaaca ttacttcttg ttatcgccat atacagactt
       61 taattctcgt cattattaaa gttctgtatt ggcgcttttt ttattcaaaa tcaaaagtcg
      121 gacagatgaa aagtaaaaaa cttttccatc aattcgattt attatagaac cattatatat
      181 tgttagatct ccacaattta ttggacaaaa aaataacggc tagtgctttt accacaccaa
      241 ctgttatcga tttaaagcat aaaaaaacag ccctataata aggactgttt gaataagtta
      301 aaattacatt ttaactacgt ttgcagcttg ctcgccacgg tcgccttcaa cgatgtcgaa
      361 ttcaactttt tggccttctt ctaatgattt gtatccatct tcagcgattg ctgagaagtg
      421 tacgaatacg tcgctaccat cttctctttc gatgaaacca aaaccttttt ctgcattaaa
      481 ccatttaact gtaccgttat tcatattgaa tacctccgtc gtgcttttgc actaaatatt
      541 ttgtaacaaa ttcataaatg aaaaggagaa tattctatat aaataactca catttttctt
      601 cacgctcttt attacttaac tt
//
LOCUS       DAHHSC010000413          616 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.421, whole genome shotgun sequence.
ACCESSION   DAHHSC010000413 DAHHSC010000000
VERSION     DAHHSC010000413.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 616)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 616)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..616
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.421"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            complement(217..573)
                     /locus_tag="NBI57_002801"
     CDS             complement(217..573)
                     /locus_tag="NBI57_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HCD1823682.1"
                     /translation="MKLGMLLIRWMLATSFLVHGTLKFVDLDGTIHFLGSLGLPPTIA
                     VLMSIGEVVGGLALIIGFLTPYVNVFYICVMLGSIFTLKLTRGYVSGYEFEIIHIVMN
                     LACISSYSWKKWLQFY"
BASE COUNT          248 a          103 c           86 g          179 t
ORIGIN      
        1 aaatactaaa gcttgttaat aatgatgttt tagcagagga gtttggttcg aaagcgagag
       61 aaaacattat agaaaaatat tcaacggaat caatattaga aaaatggtta aatcttttca
      121 atagctaaat gaaaaaatcc taggtattca tgagtaccta ggatttttac gcatgatata
      181 tacaaaaaaa gagaggatag tcctctcaaa taatagttaa taaaattgta gccatttctt
      241 ccagctgtaa ctactaatac aagctaagtt cattacgata tgtatgattt caaattcata
      301 accacttaca tatcctcttg taagtttaag tgtgaagatt gagcctaaca taacacaaat
      361 ataaaaaaca ttaacataag gcgttaaaaa gccgataata agtgctaaac ctccaacaac
      421 ttcaccaatt gacatcaata ctgcgatggt aggtggtaaa cctaaagagc ctaagaagtg
      481 aattgttcca tctaaatcaa caaatttcaa agtaccatgc actaagaatg atgttgctaa
      541 catccatcta attaaaagca tacctaattt catcatgtca cctccaacat tcatattttt
      601 aaaacatagt ccaaat
//
LOCUS       DAHHSC010000414          614 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.422, whole genome shotgun sequence.
ACCESSION   DAHHSC010000414 DAHHSC010000000
VERSION     DAHHSC010000414.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 614)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 614)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..614
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.422"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            85..582
                     /gene="efb"
                     /locus_tag="NBI57_002802"
     CDS             85..582
                     /gene="efb"
                     /locus_tag="NBI57_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="complement convertase inhibitor Efb"
                     /protein_id="HCD1823683.1"
                     /translation="MKNKLIAKSLLTIAAIGITTTTIASTADASEGYGPREKKPVSIN
                     HNIVEYNDGTFKYQSRPKFNSTPKYIKFKHDYNILEFNDGTFEYGARPQFNKPAAKTD
                     ATIKKEQKLIQAQNLVREFEKTHTVSAHRKAQKAVNLVSFEYKVKKMVLQERIDNVLK
                     QGLVR"
BASE COUNT          265 a           82 c           95 g          172 t
ORIGIN      
        1 ttaattaata attagttaat ttcaaaagtt gtataaatag gataacttaa taaatgtaag
       61 ataataattt ggaggataat taacatgaaa aataaattga tagcaaaatc tttattaaca
      121 atagcggcaa ttggtattac tacaactaca attgcgtcaa cagcagatgc gagcgaagga
      181 tacggtccaa gagaaaagaa accagtgagt attaatcaca atatcgtaga gtacaatgat
      241 ggtactttta aatatcaatc tagaccaaaa tttaactcaa cacctaaata tattaaattc
      301 aaacatgact ataatatttt agaatttaac gatggtacat tcgaatatgg tgcacgtcca
      361 caatttaata aaccagcagc gaaaactgat gcaactatta aaaaagaaca aaaattgatt
      421 caagctcaaa atcttgtgag agaatttgaa aaaacacata ctgtcagtgc acacagaaaa
      481 gcacaaaagg cagtcaactt agtttcgttt gaatacaaag tgaagaaaat ggtcttacaa
      541 gagcgaattg ataatgtatt aaaacaagga ttagtgagat aatacttctg tcattatttt
      601 aagttcaaaa taat
//
LOCUS       DAHHSC010000415          601 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.424, whole genome shotgun sequence.
ACCESSION   DAHHSC010000415 DAHHSC010000000
VERSION     DAHHSC010000415.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 601)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 601)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..601
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.424"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            1..601
                     /locus_tag="NBI57_002803"
                     /pseudo
     CDS             1..601
                     /locus_tag="NBI57_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002455976.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-directed DNA polymerase"
BASE COUNT          256 a           60 c           89 g          196 t
ORIGIN      
        1 aagactttac tttacggtgg taatcatata ttaaacgttg atttatctaa tttttatcat
       61 tctctttata cacatagcat accttgggta ataatgggaa agaagaatgc taaaaaagaa
      121 agaaaggttt ttcaaatcaa ttagataaat taattacatc ctgtcaatat aatcaaactc
      181 atggaatacc tactggcaac atattaagtc gtattattag tgaattatat atgtgttata
      241 ttgattctga aatggaaaat aaagggtata gatatgcgag atatgtggat gacatatcat
      301 tctcatttaa ttttgaagaa gagaaagata aattttacag agattttaac aaactatgta
      361 tgaaatatga attaaaaatt aatgacaaga aaactgaagt aaatgatttt ccatatatac
      421 atccacaaaa caaagacttt atatttaatt atttcaaaaa ttattcctca aatagtaaag
      481 atgaaacttg gattataggt attaagaact ttattgattt atgtatagat gaagaaagaa
      541 aaggaaataa aggggcaata aaaagtattt tcccagtaat agaaaatact ttaaagaaga
      601 a
//
LOCUS       DAHHSC010000416          597 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.425, whole genome shotgun sequence.
ACCESSION   DAHHSC010000416 DAHHSC010000000
VERSION     DAHHSC010000416.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 597)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 597)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..597
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.425"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            complement(<1..>597)
                     /locus_tag="NBI57_002804"
     rRNA            complement(<1..>597)
                     /locus_tag="NBI57_002804"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          123 a          184 c          124 g          166 t
ORIGIN      
        1 tcgtttacgg cgtggactac cagggtatct aatcctgttt gatccccacg ctttcgcaca
       61 tcagcgtcag ttacagacca gaaagtcgcc ttcgccactg gtgttcctcc atatctctgc
      121 gcatttcacc gctacacatg gaattccact ttcctcttct gcactcaagt tttccagttt
      181 ccaatgaccc tccacggttg agccgtgggc tttcacatca gacttaaaaa accgcctacg
      241 cgcgctttac gcccaataat tccggataac gcttgccacc tacgtattac cgcggctgct
      301 ggcacgtagt tagccgtggc tttctgatta ggtaccgtca agatgtgcac agttacttac
      361 acatatgttc ttccctaata acagagtttt acgatccgaa gaccttcatc actcacgcgg
      421 cgttgctccg tcaggctttc gcccattgcg gaagattccc tactgctgcc tcccgtagga
      481 gtctggaccg tgtctcagtt ccagtgtggc cgatcaccct ctcaggtcgg ctatgcatcg
      541 ttgccttggt aagccgttac cttaccaact agctaatgca gcgcggatcc atctata
//
LOCUS       DAHHSC010000417          595 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.426, whole genome shotgun sequence.
ACCESSION   DAHHSC010000417 DAHHSC010000000
VERSION     DAHHSC010000417.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 595)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 595)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..595
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.426"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            <1..>595
                     /locus_tag="NBI57_002805"
     CDS             <1..>595
                     /locus_tag="NBI57_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HCD1823684.1"
                     /translation="ITFFLLEQFNTMQHMGLVEKIGNSFFQSVTTRTAGFNSIDIASI
                     NKSTALMLMLLMFIGGAPLSAAGGIKITTFAVAFIFVLNYIRKENNVSVFNKEISDKH
                     IKLSIVTINISFLFISIITFILSIINPNISLIKLLFEVVSAFGTVGLSMNLTTEYHGI
                     TKIIIIFVMLCGKVGLLTLLRTFIPPKSPKNYRYTKGQ"
BASE COUNT          211 a           92 c           82 g          210 t
ORIGIN      
        1 tattacattc tttttattag aacagtttaa tactatgcaa catatgggac tagttgaaaa
       61 aatcggaaat tcttttttcc aatcagtaac aacacgaaca gcgggtttta acagtattga
      121 tatagcaagc attaacaaat ctaccgcctt aatgttaatg ttacttatgt ttattggtgg
      181 tgcccctctc agtgcagctg gaggaattaa aataactact tttgcagtgg cgtttatatt
      241 tgttttaaat tatatacgta aagaaaataa tgtttcagta ttcaataaag aaatatctga
      301 caaacatata aaactatcta ttgttaccat taatatctca tttctattta tcagtatcat
      361 tacttttata ttatcgataa ttaatccgaa catatcatta atcaagttat tattcgaagt
      421 ggtttctgca ttcggaacag tcgggttaag tatgaacctt accacagaat atcatggtat
      481 tactaaaata attattatat tcgttatgct ttgcggtaaa gtaggactat taactttatt
      541 aagaacattt ataccaccaa aaagtcctaa aaattaccgc tacactaaag gacaa
//
LOCUS       DAHHSC010000418          588 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.427, whole genome shotgun sequence.
ACCESSION   DAHHSC010000418 DAHHSC010000000
VERSION     DAHHSC010000418.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 588)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 588)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..588
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.427"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            complement(249..449)
                     /gene="cspA"
                     /locus_tag="NBI57_002806"
     CDS             complement(249..449)
                     /gene="cspA"
                     /locus_tag="NBI57_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015900294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock protein CspA"
                     /protein_id="HCD1823685.1"
                     /translation="MKQGTVKWFNAEKGFGFIEVEGENDVFVHFSAINQDGYKSLEEG
                     QAVEFEVVEGDRGPQAANVVKL"
BASE COUNT          207 a          108 c           74 g          199 t
ORIGIN      
        1 atacaccgaa aactaataaa ttagcaacta aactaaggat aagatatttc ataaaatcac
       61 ctcgtcgttg tacaccataa ttatattata taaatataat tttctattgc aaacaatgtt
      121 ggtgatataa aaaaagagtt ctcaatttgg ctgagaaact catcgcgtta agtatttgta
      181 ataagcatat aaaaacacct tacttcttgg taaggtgtta gttaaaatca atgattcaaa
      241 tctaagaatt atagtttaac aacgtttgca gcttgtggac cgcggtcgcc ttcaactact
      301 tcaaactcaa cagcttgacc ttcttctaat gatttgtaac catcttggtt aattgctgaa
      361 aaatgtacga atacgtcatt ttctccttca acttcgataa agccgaatcc tttttcagcg
      421 ttaaaccatt taactgtacc ttgtttcata atctgaaacc tccaagacta aaattcattc
      481 aatatgctta ttcgcatatt acaaaaaata catgcaaatt tcactcaaat attctttgca
      541 atatggaata atcatctgct taacttgtat tatagtgcag ttattacc
//
LOCUS       DAHHSC010000419          576 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.428, whole genome shotgun sequence.
ACCESSION   DAHHSC010000419 DAHHSC010000000
VERSION     DAHHSC010000419.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 576)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 576)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..576
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.428"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            <1..246
                     /locus_tag="NBI57_002807"
     CDS             <1..246
                     /locus_tag="NBI57_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1823686.1"
                     /translation="LNAINIVITTTFVIFNILITYNKDLDDLCWLLPGIIICGVILIV
                     SFTIAMITKNWLSEILFFINIVLVLYYIYPIFYSFIG"
     gene            275..463
                     /locus_tag="NBI57_002808"
     CDS             275..463
                     /locus_tag="NBI57_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1823687.1"
                     /translation="MSLHFAILFWLALIFLVAATFILVLMKKTGKESKKESYLSFTVI
                     LYIFGFAILIYTFIFGVL"
BASE COUNT          173 a           81 c           74 g          248 t
ORIGIN      
        1 ttaaatgcta tcaatatagt tattactacc acttttgtta tctttaatat tttaatcaca
       61 tataataaag atttagatga tttatgttgg ctcctgcctg gtattatcat ttgtggtgtg
      121 atactcatcg tatcctttac cattgcaatg ataactaaaa actggttaag tgaaatatta
      181 ttttttataa atatcgtact cgttctctat tacatttatc ctatttttta tagttttata
      241 ggttaataat tacggtatat aaggagggtt tcacatgtct ttacattttg caattctgtt
      301 ttggctagca ttaattttct tagttgccgc tacgtttata ctcgtattaa tgaaaaaaac
      361 tggcaaagaa tctaaaaaag agtcctattt aagtttcact gtcattctct atatttttgg
      421 attcgctata ttaatataca catttatatt tggtgtgcta taaatgaaat tcattttata
      481 tccagtctga aacgtctcaa tgatgtttca gactgttttt tattcttaat agacatttca
      541 cactctcata ctttaatatt taagatatgt tttagt
//
LOCUS       DAHHSC010000420          574 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.429, whole genome shotgun sequence.
ACCESSION   DAHHSC010000420 DAHHSC010000000
VERSION     DAHHSC010000420.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 574)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 574)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..574
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.429"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            150..296
                     /locus_tag="NBI57_002809"
     CDS             150..296
                     /locus_tag="NBI57_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1823688.1"
                     /translation="MNGGENFMVDKNKKQETTRSNPLNKSFEKSGASEKLKSTLSEKA
                     KKKD"
     gene            complement(293..>574)
                     /locus_tag="NBI57_002810"
     CDS             complement(293..>574)
                     /locus_tag="NBI57_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HCD1823689.1"
                     /translation="KLDCISLLDKRDKLFNNNGKPVFIVIKDFENRIIEEGELKTYNS
                     AGSDFDLLEVERQDFKVSDLPSNDELYIKHTLVDLKQQIKLDLYLMNEY"
BASE COUNT          215 a           88 c           77 g          194 t
ORIGIN      
        1 gacatgaaaa aagcaatctt aactttaagt cttatattta ttacctacta cctcactttt
       61 aaatatatgt ggattaaaga attgaagtat taacagcttt ttatagccct ttaatataaa
      121 aatcaaaaaa acgcctacta gtgtagacgt tgaatggtgg tgagaatttt atggtagata
      181 aaaacaaaaa acaagaaact actcgtagta acccattaaa caaaagtttt gaaaagtcag
      241 gcgccagcga aaaattaaaa agcactttat cagaaaaagc taagaaaaaa gattagtatt
      301 cattcattaa atataaatcc aatttaattt gttgtttaag gtctacaagc gtatgtttaa
      361 tatacaattc atcgtttgac ggtaaatcag atactttgaa atcttgtcgc tcaacttcta
      421 gtaaatcgaa atcgctacca gctgaattat aggttttaag ttcaccctct tcaatgattc
      481 tgttttcaaa gtcttttata actataaata ctggtttacc gttgttatta aacaacttgt
      541 ctcttttgtc taataagctt atacaatcca attt
//
LOCUS       DAHHSC010000421          567 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.430, whole genome shotgun sequence.
ACCESSION   DAHHSC010000421 DAHHSC010000000
VERSION     DAHHSC010000421.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 567)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 567)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..567
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.430"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            99..533
                     /locus_tag="NBI57_002811"
     CDS             99..533
                     /locus_tag="NBI57_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000769690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAP domain-containing protein"
                     /protein_id="HCD1823690.1"
                     /translation="MKLKSFITVTLALGMIATTGATVTSNEVSAAEKDKLPATQKAKE
                     MQNVPYTIAVDGIMAFNQSYLNLPKDSQLSYLDLGNKVKALLYDERGVTPEKIRNAKS
                     AVYTITWKDGSKKEVDLKKDSYTANLFDSNSIKQIDINVKTK"
BASE COUNT          228 a           79 c           91 g          169 t
ORIGIN      
        1 acaagtaaac aatttacata cttataattt ttgaaacttt tagatttgtg ttatattgat
       61 tttgtaagat actttaactc acaaaggaga gagattatat gaaattaaaa tcatttataa
      121 ctgtaacttt ggcactgggc atgatcgcaa cgactggcgc tactgtgaca agtaatgagg
      181 tatctgcagc agaaaaggac aaactaccgg caactcaaaa agctaaagaa atgcaaaatg
      241 ttccatatac aattgcagta gatggcatta tggctttcaa tcaatcttac ttaaatttac
      301 caaaagatag ccaattatca tatttagatt taggaaataa agttaaagct ttattatatg
      361 atgaacgtgg tgtaacacct gagaagattc gaaatgcaaa atctgccgtt tacacgatta
      421 cttggaaaga tggtagtaaa aaagaagtgg atcttaagaa agatagctac acagcaaact
      481 tgtttgattc aaattcaatt aaacaaattg atattaatgt aaaaactaaa taaacactta
      541 aaattatcaa attcaacatg aagcatt
//
LOCUS       DAHHSC010000422          556 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.431, whole genome shotgun sequence.
ACCESSION   DAHHSC010000422 DAHHSC010000000
VERSION     DAHHSC010000422.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 556)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 556)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..556
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.431"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            <1..>556
                     /locus_tag="NBI57_002812"
     rRNA            <1..>556
                     /locus_tag="NBI57_002812"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          159 a          126 c          159 g          112 t
ORIGIN      
        1 cactgccgag aaaagcctct agatagaaaa taggtgcccg taccgcaaac cgacacaggt
       61 agtcaagatg agaattctaa ggtgagcgag cgaactctcg ttaaggaact cggcaaaatg
      121 accccgtaac ttcgggagaa ggggtgctct ttagggttaa cgcccagaag agccgcagtg
      181 aataggccca agcgactgtt tatcaaaaac acaggtctct gctaaaccgt aaggtgatgt
      241 ataggggctg acgcctgccc ggtgctggaa ggttaagagg agtggttagc ttctgcgaag
      301 ctacgaatcg aagccccagt aaacggcggc cgtaactata acggtcctaa ggtagcgaaa
      361 ttccttgtcg ggtaagttcc gacccgcacg aaaggcgtaa cgatttgggc actgtctcaa
      421 cgagagactc ggtgaaatca tagtacctgt gaagatgcag gttacccgcg acaggacgga
      481 aagaccccgt ggagctttac tgtagcctga tattgaaatt cggcacagct tgtacaggat
      541 aggtaggagc ctttga
//
LOCUS       DAHHSC010000423          551 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.432, whole genome shotgun sequence.
ACCESSION   DAHHSC010000423 DAHHSC010000000
VERSION     DAHHSC010000423.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 551)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 551)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..551
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.432"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            16..315
                     /locus_tag="NBI57_002813"
     CDS             16..315
                     /locus_tag="NBI57_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000844410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGE"
                     /protein_id="HCD1823691.1"
                     /translation="MKSYKCKGSFLIDSMAGFLLIGLITLLLIPMMNQMQASINHKLQ
                     SIDASKVILTTVSKINKEELKKGVTIGKYDIKQSDQQICAISKNTTSYQKTCIQY"
     gene            233..>551
                     /locus_tag="NBI57_002814"
     CDS             233..>551
                     /locus_tag="NBI57_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HCD1823692.1"
                     /translation="MILSKVTNKFVLFQKIPLLIKRHVYSINVKAFSLIEMLVAMMVI
                     SIILLIVPDLIRLSKTFLIESRELTTVDFEFFSRDILEDFKGVDKNDIEIRQQRIILH
                     KGEE"
BASE COUNT          215 a           63 c           96 g          177 t
ORIGIN      
        1 aaggaagaat tcgttatgaa aagctataag tgtaaaggat cattcttaat agatagtatg
       61 gctggatttt tgctaattgg attgattaca ttactattga taccaatgat gaatcaaatg
      121 caagcgagta taaaccataa actacaatca attgatgctt ctaaagtaat tttgacgact
      181 gtatctaaaa ttaataaaga agaacttaag aagggggtaa ctatagggaa atatgatatt
      241 aagcaaagtg accaacaaat ttgtgctatt tcaaaaaata ccacttctta tcaaaagaca
      301 tgtatacagt attaatgtca aagctttttc gcttattgaa atgttagtag cgatgatggt
      361 tataagtata attttactaa ttgttccaga cttaattaga cttagcaaaa cttttctaat
      421 tgaaagtagg gaattaacaa ctgtagattt cgaatttttc tcaagagata ttctagagga
      481 ttttaaaggt gtagataaaa acgatattga aattaggcaa cagcgtatca ttttacataa
      541 aggtgaagaa a
//
LOCUS       DAHHSC010000424          535 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.433, whole genome shotgun sequence.
ACCESSION   DAHHSC010000424 DAHHSC010000000
VERSION     DAHHSC010000424.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 535)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 535)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..535
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.433"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            <1..>535
                     /locus_tag="NBI57_002815"
     CDS             <1..>535
                     /locus_tag="NBI57_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA"
                     /protein_id="HCD1823693.1"
                     /translation="DILINNKYVIELQYSPIPYKQILQRTEGLKKMGYKVSWLLNDVD
                     YCHNKVKFNHFHSLFINPITRKLHTFNLEKKQIMMFQQIQYLGGHKYVAEKRNAKIIE
                     LFNEAPCDYHAVYKLSKFAINQYIKYCRWQNSVLEPTLSAMYQLQLTDQEVVYNYGYI
                     FPEQIYIENHPIEWQLQV"
BASE COUNT          198 a           68 c           86 g          183 t
ORIGIN      
        1 gatattttga ttaataataa atatgttatt gagctacagt attcgccaat tccttataaa
       61 cagattcttc aacgaacgga aggtttaaag aaaatgggat ataaagtaag ttggttatta
      121 aatgatgttg attattgtca taataaagtg aagttcaatc attttcatag tttgtttatt
      181 aatccaatca ctcgaaaact tcatacgttc aatttagaga aaaaacaaat aatgatgttt
      241 caacaaatac aatatttagg cgggcacaaa tatgtcgctg aaaaaaggaa tgccaaaatt
      301 attgagttgt ttaatgaggc gccttgtgat tatcatgctg tttataaatt atcaaagttc
      361 gcaattaatc aatatatcaa atattgtcgc tggcaaaatt ctgttttaga acccacttta
      421 agtgcaatgt atcaattaca gttaactgat caagaagtag tgtacaatta tggttatatt
      481 tttccagagc aaatttatat tgaaaatcat ccaattgagt ggcaattaca agttg
//
LOCUS       DAHHSC010000425          531 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.434, whole genome shotgun sequence.
ACCESSION   DAHHSC010000425 DAHHSC010000000
VERSION     DAHHSC010000425.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 531)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 531)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..531
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.434"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            <1..>531
                     /locus_tag="NBI57_002816"
     CDS             <1..>531
                     /locus_tag="NBI57_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCD1823694.1"
                     /translation="GNVNANSGEIFDKDKLLQSENRSIMFQKTMFPDQLKVIEIINLY
                     QSFYENPLPLEEIIELTKFDSSQLNQFVNKLSGGQQRLLDFVLSLIGQPQLILLDEPT
                     STMDIEIREYFWSIIENLKEDNRTILYTSHYIEEVERMSDKIILIENGEIILNDSTSH
                     IRTNQQSQITLSDEYI"
BASE COUNT          209 a           76 c           79 g          167 t
ORIGIN      
        1 tggtaatgtt aatgctaatt ctggtgagat atttgataaa gacaagttat tacaaagtga
       61 aaatcgcagt ataatgttcc aaaaaacgat gtttccagat caattaaaag ttattgagat
      121 tatcaactta tatcaatcat tttacgaaaa tccattacca ttggaagaaa taatagaact
      181 gacgaaattt gattctagtc aactgaacca atttgtaaat aaactttctg gtggtcaaca
      241 acgattactc gattttgtat tatctttaat cggacaacca caattgatct tattagatga
      301 accaacatcg actatggata tagaaattag agaatatttt tggtcaatta ttgaaaattt
      361 aaaagaagat aatcgaacga tactctatac atcgcactat attgaagaag tcgaacgtat
      421 gtcagacaaa attattctca ttgaaaatgg agaaataata cttaatgatt caacgtcaca
      481 tattagaacc aatcagcaat ctcagattac gttatccgat gaatatataa g
//
LOCUS       DAHHSC010000426          530 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.435, whole genome shotgun sequence.
ACCESSION   DAHHSC010000426 DAHHSC010000000
VERSION     DAHHSC010000426.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 530)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 530)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..530
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.435"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            complement(<1..>530)
                     /locus_tag="NBI57_002817"
     CDS             complement(<1..>530)
                     /locus_tag="NBI57_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF443 family protein"
                     /protein_id="HCD1823695.1"
                     /translation="STWISLFFPFINWFIPKRYVKISREEFENLNIVKPAKKNVFWPV
                     ASISTLFAVTLRKYTLLLDTQLDKKLVIAICCTTFIGILIFYVRLIKKSSLNIYNTKN
                     KRSKIILIPTLKNFCFTLFGYVFLIFWTVIFSYALLSMSYQNIIVYFAWITAIIGFFL
                     VNIALIIDKNIHVILK"
BASE COUNT          208 a           66 c           69 g          187 t
ORIGIN      
        1 ttttaagtat gacatgaatg tttttatcta taattaaagc tatattcact agaaaaaaac
       61 ctattattgc tgtaatccaa gcaaaatata ctattatgtt ttgataactc attgataata
      121 gagcatatga gaaaatcact gtccaaaaaa ttaaaaaaac atatccgaac aatgtgaaac
      181 aaaaattttt tagtgttggt attaagatga ttttcgacct tttattttta gtattataaa
      241 tatttaaaga tgatttttta attaggcgta cataaaatat taaaatccct ataaatgtgg
      301 tgcaacatat ggcaataact aattttttat caagttgagt gtcaagtaaa agcgtatact
      361 ttcttaatgt aactgcgaat aaagtagaga tactagcaac tggccaaaaa acattctttt
      421 tagcaggttt aacaatattt aaattttcaa actcttctct actaattttg acgtatcttt
      481 tggggatgaa ccaattaata aaaggaaaaa ataaactaat ccaagtgctt
//
LOCUS       DAHHSC010000427          528 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.436, whole genome shotgun sequence.
ACCESSION   DAHHSC010000427 DAHHSC010000000
VERSION     DAHHSC010000427.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 528)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 528)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..528
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.436"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            complement(75..>528)
                     /locus_tag="NBI57_002818"
     CDS             complement(75..>528)
                     /locus_tag="NBI57_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1823696.1"
                     /translation="VTNRKKPFLFLKTLFMGVVFIFIGYISLAISAVIIYGIIQFITI
                     DFGSFFLMGIILILISSIFQLFIVRLLFRKKNVDLTEVVVLEHLIQWFLVYFAIYQAV
                     NEKMDINDINIDNFQSVFFDVSNLNLVILPTLIISWVTIFNYRMRSYK"
BASE COUNT          216 a           83 c           64 g          165 t
ORIGIN      
        1 ttcaaaaata aaaccacaca acaaaaaaag acaccatttg aatgttggtg tctgatggta
       61 taatttcata gattttattt gtaacttctc attctatagt taaatattgt gacccagcta
      121 atgattaacg ttggtagaat tactaaattc aaattagaca cgtcaaaaaa gacagattgg
      181 aaattgtcga tattaatatc attaatgtcc attttttcat ttactgcttg atagatcgca
      241 aagtaaacta agaaccattg aattaaatgc tctaaaacga caacctctgt caaatcgaca
      301 ttcttttttc taaaaagtaa tctaactata aataattgga atatacttga aatcaagatc
      361 aatataatac ccattaagaa aaaactacca aaatcaattg tgataaattg aataatacca
      421 taaataatta cggcagatat tgccagtgaa atatatccta taaagataaa taccacaccc
      481 ataaagagtg tctttaagaa cagaaaaggc ttctttctat ttgtaacc
//
LOCUS       DAHHSC010000428          515 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.437, whole genome shotgun sequence.
ACCESSION   DAHHSC010000428 DAHHSC010000000
VERSION     DAHHSC010000428.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 515)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 515)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..515
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.437"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            <1..>515
                     /locus_tag="NBI57_002819"
     CDS             <1..>515
                     /locus_tag="NBI57_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HCD1823697.1"
                     /translation="NSQPLNEYIDGMKTNQDTVLSIYQSFELPEDDSRIKKIKEMNYS
                     KVLVITEDWCGDAMMNLPILKHISEALNLEVRVFHRDDDTKLIDQYLTNGKSRAIPIF
                     VFLNDQFEQETVWGPRANEVQKFVTDVRADKLPSKDHPDYADLEKETHLVISNRYKTD
                     ASFWKAVYNSIL"
BASE COUNT          200 a           87 c           86 g          142 t
ORIGIN      
        1 aatagccaac ccttaaatga atacattgat ggaatgaaga caaatcaaga taccgttctc
       61 agtatctatc aatcatttga gttaccagaa gatgattcac gcatcaaaaa aataaaagag
      121 atgaattatt ctaaagtatt agtaattact gaagactggt gtggagatgc aatgatgaat
      181 ctcccaattc taaaacacat cagtgaagca ttaaatttag aagtacgcgt gttccataga
      241 gatgatgata caaaactgat cgatcaatat ttaacaaatg gtaaatcacg tgcaattcct
      301 atcttcgtat tcttgaatga tcaattcgaa caagaaacag tttggggacc aagagcaaat
      361 gaagtccaaa aatttgtaac tgatgttcgt gctgataaat taccgagcaa agatcatcca
      421 gactatgcag atttagaaaa agaaacacac ttagttatat caaaccgcta taaaactgac
      481 gcttcatttt ggaaagcagt ttataattcc atcct
//
LOCUS       DAHHSC010000429          508 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.438, whole genome shotgun sequence.
ACCESSION   DAHHSC010000429 DAHHSC010000000
VERSION     DAHHSC010000429.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 508)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 508)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..508
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.438"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            complement(33..479)
                     /gene="perR"
                     /locus_tag="NBI57_002820"
     CDS             complement(33..479)
                     /gene="perR"
                     /locus_tag="NBI57_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide-responsive transcriptional repressor
                     PerR"
                     /protein_id="HCD1823698.1"
                     /translation="MSVEIESIEHELEESIASLRQAGIRITPQRQAILRYLISSHTHP
                     TADEIYQALSPDFPNISVATIYNNLRVFKDIGIVKELTYGDSSSRFDFNTHNHYHIIC
                     EQCGKIVDFQYPQLNEIERLAQHMTDFDVTHHRMEIYGVCKECQDK"
BASE COUNT          158 a           81 c           67 g          202 t
ORIGIN      
        1 cttttttaat ttgtcatact accaaagtta aattatttat cttggcattc tttacaaact
       61 ccataaattt ccattcgatg atgtgttacg tcaaagtcag tcatatgctg agctaatctt
      121 tcaatttcat ttaactgtgg atattgaaaa tcaacaatct taccacattg ttcacatata
      181 atatgataat gattatgtgt attaaagtcg aatcgacttg atgagtctcc atatgttaat
      241 tcttttacaa ttccaatatc tttaaacact cttaagttat tatatattgt cgcaacactt
      301 atatttggaa aatcaggtga aagtgcttga taaatttcat cagctgttgg atgagtatgt
      361 gaagaaatta aataacgtaa tattgcttgt ctttgaggtg taattcttat gcctgcttgt
      421 cgcaatgatg caattgattc ttctagttca tgttcaattg attctatttc aacactcatc
      481 tatatcacca tctttcttat ataataat
//
LOCUS       DAHHSC010000430          497 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.440, whole genome shotgun sequence.
ACCESSION   DAHHSC010000430 DAHHSC010000000
VERSION     DAHHSC010000430.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 497)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 497)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..497
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.440"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            <1..440
                     /locus_tag="NBI57_002821"
     CDS             <1..440
                     /locus_tag="NBI57_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="EMYY motif lipoprotein"
                     /protein_id="HCD1823699.1"
                     /translation="KQFEKNRYKVESEIKLADNKSEATNLTTKLEHNNKALRDTAKKN
                     LDDSKENEVKGAIKNHIMPMIEKQITDINQTNISDKHVNNARKNAIEMYYSLQNYYNT
                     RIETIKVSEKLSKVDVDKLPKKGIDITHGDKAFEKKLEKLEEK"
BASE COUNT          233 a           57 c           81 g          126 t
ORIGIN      
        1 cgaaacaatt tgaaaaaaat agatacaaag ttgaatcaga aattaaatta gctgataata
       61 aaagtgaagc aactaatctt acgacaaaat tagaacataa taataaagcg ttaagagata
      121 ctgcgaagaa gaacctagat gatagtaaag aaaatgaagt aaaaggcgcg attaaaaatc
      181 acattatgcc aatgattgaa aaacaaatta ccgatattaa ccaaactaat attagtgata
      241 agcatgttaa taatgcaagg aaaaacgcaa tagaaatgta ttacagtctg cagaactatt
      301 ataatacacg tattgaaaca ataaaggtta gtgagaagtt atcaaaagtc gatgtagata
      361 agttgccgaa aaagggtata gatataactc acggcgataa agcctttgaa aaaaagcttg
      421 aaaaattaga agaaaaataa ctataatcat ttttcaaagt taaaaatttt gaatttatgg
      481 ttaacatgtc aacttac
//
LOCUS       DAHHSC010000431          493 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.441, whole genome shotgun sequence.
ACCESSION   DAHHSC010000431 DAHHSC010000000
VERSION     DAHHSC010000431.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 493)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 493)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..493
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.441"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            <1..>493
                     /gene="seo"
                     /locus_tag="NBI57_002822"
     CDS             <1..>493
                     /gene="seo"
                     /locus_tag="NBI57_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphylococcal enterotoxin type O"
                     /protein_id="HCD1823700.1"
                     /translation="AYANEEDPKIESLCKKSSVDPIALHNINDDYINNRFTTVKSIVS
                     TTEKFLDFDLLFKSINWLDGISAEFKDLKVEFSSSAISKEFLGKTVDIYGVYYKAHCH
                     GEHQVDTACTYGGVTPHENNKLSEPKNIGVAVYKDNVNVNTFIVTTDKKKVTAQELDI
                     KVRT"
BASE COUNT          193 a           56 c           89 g          155 t
ORIGIN      
        1 gcatatgcaa atgaagaaga tcctaaaata gagagtttgt gtaagaagtc aagtgtagac
       61 cctattgctt tacataatat taatgatgat tatataaata atcgatttac gacagtaaaa
      121 tcaattgtat caactacaga aaaattctta gacttcgatt tattatttaa aagtattaat
      181 tggttagatg gaatatctgc tgaatttaaa gatttaaaag tggaatttag ctcatcagcg
      241 atttctaaag aatttctagg aaagactgtt gatatttatg gtgtttacta taaagcacat
      301 tgtcatggtg agcatcaagt ggatactgcc tgtacatatg gtggggtaac acctcatgaa
      361 aataataaat taagcgagcc taaaaatata ggagtagctg tgtataagga taatgtaaat
      421 gttaatacat ttatcgttac tacagataaa aagaaagtta ctgcacaaga acttgatatt
      481 aaagtaagaa caa
//
LOCUS       DAHHSC010000432          492 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.442, whole genome shotgun sequence.
ACCESSION   DAHHSC010000432 DAHHSC010000000
VERSION     DAHHSC010000432.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 492)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 492)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..492
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.442"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            156..>492
                     /locus_tag="NBI57_002823"
     CDS             156..>492
                     /locus_tag="NBI57_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001081004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCD1823701.1"
                     /translation="MNRLNTNYFDNFYIDWQNNEISENYVEFCQKQINNLSHIVPSLK
                     KEKRKIIDTCCGCGFHSKFLIDKGHHVISFDKSEEMLNFAYKKNGILGKRQSLDEEFP
                     ENHVDLILNL"
BASE COUNT          191 a           58 c           77 g          166 t
ORIGIN      
        1 tttagttatt ataaatcaaa aaacttatta ccaattgtag aggaggtttc taaaggttta
       61 gttacattac ccttacataa tcgtttatct atcaatgatg taaaaagggt ctgcactatt
      121 attagtaaaa gtatacagga atggagagag caataatgaa tcggctaaat acaaattact
      181 ttgataactt ttatattgat tggcagaaca atgaaatatc agaaaattat gttgaatttt
      241 gtcaaaagca aattaataac ttaagtcaca ttgttccttc attaaaaaaa gagaaaagaa
      301 aaatcatcga tacttgttgt ggctgtggtt ttcattctaa atttttgata gataaaggtc
      361 atcatgttat tagctttgac aaatctgagg aaatgttgaa ctttgcatat aaaaaaaatg
      421 gcattttagg taaaagacaa tctttagatg aagaattccc agaaaatcac gtcgacttaa
      481 ttcttaattt aa
//
LOCUS       DAHHSC010000433          488 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.443, whole genome shotgun sequence.
ACCESSION   DAHHSC010000433 DAHHSC010000000
VERSION     DAHHSC010000433.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 488)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 488)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..488
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.443"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            complement(21..413)
                     /locus_tag="NBI57_002824"
     CDS             complement(21..413)
                     /locus_tag="NBI57_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(deoxy)nucleoside triphosphate
                     pyrophosphohydrolase"
                     /protein_id="HCD1823702.1"
                     /translation="MKKVINVVGAIIFSDNKILCAQRSEEMSLPLMWEFPGGKIEKNE
                     TEKEALIREIREEMKCDLIVGDKVITTEHEYDFGIVKLTTYKCTLNKELPTLTEHKSI
                     EWLSINELDKLNWAPADIPAVNKIMTEG"
BASE COUNT          152 a           95 c           48 g          193 t
ORIGIN      
        1 catttagtaa tctactcata ttatccctca gtcataattt tattaacggc tggtatatcc
       61 gctggggccc aatttaattt atcaagttcg tttattgaca accattcaat actcttatgt
      121 tcagttaaag ttggtaactc tttgtttaaa gtacatttgt atgttgttaa cttaacaatg
      181 ccaaaatcat attcatgttc tgtagttatg actttgtctc caacaattag atcacatttc
      241 atttcttctc taatttctct aatcaaagct tctttttcag tttcattctt ttcaatctta
      301 ccgccaggaa attcccacat taaaggtaga ctcatttctt cacttctctg tgcacaaaga
      361 attttgttat cagaaaaaat aatagctcct actacattga ttactttttt cataagactc
      421 acccttcaat ttaaaatcat cttaattgtt attctatcaa aaattacaaa actatatata
      481 aatcaata
//
LOCUS       DAHHSC010000434          480 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.445, whole genome shotgun sequence.
ACCESSION   DAHHSC010000434 DAHHSC010000000
VERSION     DAHHSC010000434.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 480)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 480)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..480
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.445"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
BASE COUNT          158 a           90 c           56 g          176 t
ORIGIN      
        1 ctaccgttat gtataactta tttatatttt tgtacaagat ataatattgt ccaacttcaa
       61 atatccaaac ctattaataa taaaactaga taccatcgta ctctatcatg gctttcttat
      121 aatcgagtag aagcatcatc attacttgat tatttgctct ttacaacacc gagcgtgccc
      181 gtactcggta attcaatacc ttgcctaacc cgtcactgtg agttgggtta atgataataa
      241 agcccacacc ttttaaaaag atgtgggtaa tttatataat ttttatttac atttttaact
      301 tataaaaaaa agcgcctatg tcatgattta ccatcacata ggcgcttatc aataaattat
      361 tacttattac tttccatttc atctaattta tgcggatttc ctgtaattag atgacaactt
      421 attcttttca ggggaacatt acacttttat aatatgttca aagacaaact taaccattca
//
LOCUS       DAHHSC010000435          476 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.446, whole genome shotgun sequence.
ACCESSION   DAHHSC010000435 DAHHSC010000000
VERSION     DAHHSC010000435.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 476)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 476)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..476
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.446"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            <1..227
                     /locus_tag="NBI57_002825"
     CDS             <1..227
                     /locus_tag="NBI57_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000810447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1823703.1"
                     /translation="YDDITVNDIHVYFDTYDPKKDSYKVFVQFDERIETDGDDKIEHR
                     QTSAQLDLVRTAEGWRIDNLKRFNLKPLGR"
     gene            283..>476
                     /locus_tag="NBI57_002826"
     CDS             283..>476
                     /locus_tag="NBI57_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001255377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1823704.1"
                     /translation="MRRWFVLILGLVILLSACGQKYDKEIDAVLNSERKSMSESSFKK
                     PEKSNSDFKVYEDGKFITIS"
BASE COUNT          170 a           44 c           91 g          171 t
ORIGIN      
        1 tatatgatga tataacggtt aatgatattc atgtttattt tgatacttat gaccctaaaa
       61 aagatagtta taaagttttt gttcagttcg acgaacgtat tgaaacagat ggagatgata
      121 aaattgaaca tcgtcaaaca tctgcacaat tggatttagt tcgtacagct gaaggttggc
      181 gtattgataa tttaaaacgt tttaatttaa aacctctagg acgttagttt gtttcaagac
      241 gatagcaatg atataattta cgtaaaaagg agtgaattta tcatgaggcg atggtttgta
      301 ttaattttag gattagttat attattatct gcatgtggtc agaaatatga taaagagatt
      361 gatgcagttt taaattctga aagaaaaagt atgagtgagt cttcttttaa gaagcctgaa
      421 aaaagtaata gtgattttaa agtatatgaa gatggtaaat ttataactat ttcttt
//
LOCUS       DAHHSC010000436          465 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.447, whole genome shotgun sequence.
ACCESSION   DAHHSC010000436 DAHHSC010000000
VERSION     DAHHSC010000436.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 465)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 465)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..465
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.447"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            83..>465
                     /locus_tag="NBI57_002827"
     CDS             83..>465
                     /locus_tag="NBI57_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000554504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HCD1823705.1"
                     /translation="MHRESKYIEYKKSRKGLSNDIWSTYSAFANTEGGTIYLGIEEKK
                     IEDKKVFVSVGVEDPEKMIEDFWNALYGRSKVSQNILSNKDVKIVNIENKACIEIHVP
                     EAPYSKKPIYVDNKKDLVYKRVDDA"
BASE COUNT          184 a           38 c           99 g          144 t
ORIGIN      
        1 cgaaaatcgc gtgaaatact atggtagacg gtttgagtaa attaatggag tattttaata
       61 tttatggttt ggaggaattg cgttgcatag agaaagtaaa tatatagagt ataagaaatc
      121 acgaaaagga ttatctaatg atatttggtc tacgtatagt gcttttgcaa atactgaagg
      181 tggtactata tatttaggaa ttgaagaaaa aaagatcgag gacaaaaaag tctttgtttc
      241 agttggtgtt gaagatccag agaaaatgat tgaagatttt tggaatgcac tatatggaag
      301 aagtaaagtt agtcaaaata ttttatcaaa taaagatgtt aaaattgtta atattgaaaa
      361 taaagcgtgc attgaaattc atgtaccaga agcgccttat tcgaagaaac cgatatatgt
      421 agataataaa aaagatttag tatataaaag agttgatgat gctga
//
LOCUS       DAHHSC010000437          458 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.448, whole genome shotgun sequence.
ACCESSION   DAHHSC010000437 DAHHSC010000000
VERSION     DAHHSC010000437.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 458)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 458)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..458
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.448"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            291..395
                     /locus_tag="NBI57_002828"
     CDS             291..395
                     /locus_tag="NBI57_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1823706.1"
                     /translation="MFKVNYSILSYYPEYNIAVSWQRLREGKTIKNKI"
BASE COUNT          165 a           60 c           75 g          158 t
ORIGIN      
        1 atcgtcttga aatgctccct tcaaagtttt cattttttca atgtctactt cgaaggggca
       61 tttccattat attgttatag ctttttatat ttgtataatg aacatataag tttaagaaga
      121 tgagagtgaa ggaaataaaa aagctcaaat gtaccaaatt gttaatctta ataaatctct
      181 actttataaa gattgaatgg acattcgagc gttaatcagt caggagggaa tttcactcct
      241 acaatttaat aataatactt gcttcaacac tgtacaagga gtgatttgtt atgttcaaag
      301 tgaattattc gattttaagt tattatccag aatataatat cgcagtaagt tggcaacgtt
      361 taagagaagg aaaaacaata aaaaacaaga tttaatactg ctgcgtcatg aggcgcttga
      421 acattatttg atgaataagt ataatttcaa ctatgatt
//
LOCUS       DAHHSC010000438          455 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.449, whole genome shotgun sequence.
ACCESSION   DAHHSC010000438 DAHHSC010000000
VERSION     DAHHSC010000438.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 455)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 455)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..455
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.449"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            complement(<1..>455)
                     /locus_tag="NBI57_002829"
     CDS             complement(<1..>455)
                     /locus_tag="NBI57_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular biosynthesis protein"
                     /protein_id="HCD1823707.1"
                     /translation="YIALIQFLGLLIGLFLYYLIQNWIVCFITSELFATIYTLVKLRG
                     LTIGEYQSEDNNVVKDYVMLLSTNSLNNLNLYLDRLILLPIIGGTAVTISFLSTFIGK
                     MLATFLYPINNVVLSYISVNESDNIKKQYLKTNLFAIAALCLVMIICYP"
BASE COUNT          175 a           71 c           57 g          152 t
ORIGIN      
        1 attggataac atataatcat gactaaacat agtgcagcta tagcaaatag attagttttc
       61 aaatattgct tctttatatt gtcgctttca tttacagaaa tatatgaaag tactacatta
      121 ttaatcggat acagaaatgt agctaacatt ttcccaataa atgttgaaag aaatgatata
      181 gttacagctg ttccacctat aattggtaat aagattaatc tatctaagta gagattcaaa
      241 ttattaaggc tatttgtact cagtagcatc acataatctt tgaccacatt attatcttca
      301 ctttgatact cgcctatagt taatccccgt aatttaacca atgtatatat cgttgcaaac
      361 aattcactgg taataaaaca aacaatccag ttttggatta aataatatag aaatagtcct
      421 atcagcaaac ctaaaaattg aataagagca atata
//
LOCUS       DAHHSC010000439          454 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.450, whole genome shotgun sequence.
ACCESSION   DAHHSC010000439 DAHHSC010000000
VERSION     DAHHSC010000439.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 454)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 454)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..454
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.450"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            <1..>454
                     /locus_tag="NBI57_002830"
     CDS             <1..>454
                     /locus_tag="NBI57_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001623710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MAP domain-containing protein"
                     /protein_id="HCD1823708.1"
                     /translation="KKAYYTVYFKNGGKRILQLNSKNYTANLVHAKDVKRIEITVKTG
                     TKAKADRYVPYTIAVNGTSTPILSKLKISNKQLISYKYLNDKVKSVLKSERGISDLDL
                     KFAKQAKYTVYFKNGKKQVVNLKSDIFTPNLFSAKDIKKIDIDVKQYTK"
BASE COUNT          208 a           56 c           63 g          127 t
ORIGIN      
        1 taagaaagct tattatactg tttattttaa aaatggtggt aaaagaatct tacagttaaa
       61 ttcaaaaaat tacacagcaa acttagttca tgcgaaagat gttaagagaa ttgaaattac
      121 tgttaaaaca ggaactaaag cgaaagcaga cagatatgta ccatacacaa ttgcagtaaa
      181 tggcacatca acaccaattt tatcaaaact aaaaatttcg aataaacaat taattagtta
      241 caaatattta aacgacaaag tgaaatctgt attaaaaagt gaaagaggta tcagtgatct
      301 tgacttaaaa tttgcgaaac aagcaaaata tacagtatat ttcaaaaatg gaaagaaaca
      361 agtagtgaat ttaaaatcag acatctttac acctaattta tttagtgcca aagatattaa
      421 aaagattgat attgatgtaa aacaatacac taaa
//
LOCUS       DAHHSC010000440          454 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.451, whole genome shotgun sequence.
ACCESSION   DAHHSC010000440 DAHHSC010000000
VERSION     DAHHSC010000440.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 454)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 454)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..454
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.451"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            <1..237
                     /locus_tag="NBI57_002831"
     CDS             <1..237
                     /locus_tag="NBI57_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGC"
                     /protein_id="HCD1823709.1"
                     /translation="LLILIIPNIAKQTAHIQSTGCNAQVKMVNSQIEAYALKHNRNPS
                     SIEDLIADGFIKEAQKTCKSGETITISNGEAVAN"
     gene            215..>454
                     /locus_tag="NBI57_002832"
     CDS             215..>454
                     /locus_tag="NBI57_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HCD1823710.1"
                     /translation="MEKQLQIRKQSAFTMIEMLVVMMLISIFLLLTMTSKGLSNLRVI
                     DDEANIISFITELNYIKSQAIANQGYINVRFYENSD"
BASE COUNT          179 a           57 c           78 g          140 t
ORIGIN      
        1 ttattaattt taatcattcc aaatattgct aaacaaactg ctcacataca atcaacaggt
       61 tgtaatgcac aggtaaaaat ggttaatagt caaattgaag cgtatgcatt gaaacataat
      121 agaaatccat cgtctattga agacttaatt gcagatggtt ttataaaaga agcacaaaag
      181 acatgtaaat caggagagac aataacaatt agtaatggag aagcagttgc aaattagaaa
      241 gcagtcagca tttactatga ttgagatgct tgtggtaatg atgttaatca gtatatttct
      301 acttttgaca atgacatcta aaggattaag caatcttaga gtaatagatg atgaggcaaa
      361 tatcatttct tttattactg aattgaatta tattaagtcg caagctatag caaatcaagg
      421 atatatcaat gttagatttt atgaaaacag tgac
//
LOCUS       DAHHSC010000441          451 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.452, whole genome shotgun sequence.
ACCESSION   DAHHSC010000441 DAHHSC010000000
VERSION     DAHHSC010000441.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 451)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 451)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..451
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.452"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            complement(165..389)
                     /locus_tag="NBI57_002833"
     CDS             complement(165..389)
                     /locus_tag="NBI57_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1823711.1"
                     /translation="MDYAHLNLEHFFARNDDLDVIRDRADFVMINNFTNEMMYRDGQI
                     EGTIDLNQYYYKNRSNAASFIMMDYKKETK"
BASE COUNT          156 a           75 c           43 g          177 t
ORIGIN      
        1 aaataaacag taatgtaaat gaaacgtttt aaaccattta tcaacattac tttacgttga
       61 tctacgaatc ataaatgttt tatctttttg tataaccttt gaaaaccttt atatcaataa
      121 tcttattatt cacgtattaa aaaacaaaaa attcttttgt tcgtttactt agtttctttt
      181 ttataatcca tcataataaa acttgctgca tttgatctat ttttataata gtactgattt
      241 aaatcaatcg tgccttcaat ttgaccatca cgatacatca tttcattagt gaagttattt
      301 atcatcacga aatcagcgcg atctcttata acatctaaat cgtcgtttcg tgcaaaaaaa
      361 tgttctaaat ttaaatgtgc gtaatccatt gttacctccc gattacaaat tttgttctat
      421 ctactattta gcaaagtaat aatacatcat a
//
LOCUS       DAHHSC010000442          444 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.453, whole genome shotgun sequence.
ACCESSION   DAHHSC010000442 DAHHSC010000000
VERSION     DAHHSC010000442.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 444)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 444)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..444
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.453"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            <1..>444
                     /gene="sen"
                     /locus_tag="NBI57_002834"
     CDS             <1..>444
                     /gene="sen"
                     /locus_tag="NBI57_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001622180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="staphylococcal enterotoxin type N"
                     /protein_id="HCD1823712.1"
                     /translation="AEVDKKDLKKKSDLDSSKLFNLTSYYTDITWQLDESNKISTDQL
                     LNNTIILKNIDISVLKTSSLKVEFNSSDLANQFKGKNIDIYGLYFGNKCVGLTEEKTS
                     CLYGGVTIHDGNQLDEEKVIGVNVFKDGVQQEGFVIKTKKAKVTV"
BASE COUNT          191 a           45 c           76 g          132 t
ORIGIN      
        1 atgctgaagt agacaaaaaa gatttaaaga aaaaatctga tctagatagt agtaagttat
       61 ttaatttaac aagctattat actgatataa cgtggcaatt agacgagtca aataaaatta
      121 gtacagatca actactgaat aatactataa tattaaaaaa tattgatata tccgtactta
      181 aaacttctag tttgaaagtt gagtttaact catcagattt agcaaatcaa tttaaaggaa
      241 aaaatataga tatttatgga ctgtattttg gaaataaatg tgtaggctta actgaagaaa
      301 aaacatcatg cttatacgga ggagttacga tacatgatgg aaatcaatta gatgaagaga
      361 aagttatagg cgttaatgta tttaaagatg gtgtccaaca agaaggtttt gttataaaaa
      421 ccaaaaaggc taaagtaaca gtac
//
LOCUS       DAHHSC010000443          435 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.454, whole genome shotgun sequence.
ACCESSION   DAHHSC010000443 DAHHSC010000000
VERSION     DAHHSC010000443.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 435)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 435)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..435
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.454"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            complement(<1..378)
                     /gene="sarU"
                     /locus_tag="NBI57_002835"
     CDS             complement(<1..378)
                     /gene="sarU"
                     /locus_tag="NBI57_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator SarU"
                     /protein_id="HCD1823713.1"
                     /translation="MDYQTFEKVNKFINVEAYIFFLTQELKQQYKLSLKELLILAYFY
                     YKNEHSISLKEIIGDILYKQSDVVKNIKSLSKKGFINKSRNEADERRIFVSVTPIQRK
                     KIACVINELDKIIKGFNKERDYIK"
BASE COUNT          134 a           65 c           49 g          187 t
ORIGIN      
        1 ttttatgtag tctctttcct tattaaatcc tttaattatt ttatctaact cattaataac
       61 acaagcaatc tttttacgtt gtattggagt aactgaaaca aaaatacggc gttcatctgc
      121 ttcgtttcta gacttattta taaatccttt tttagatagt gacttaatgt tctttacaac
      181 atcagattgt ttgtaaagta tgtcaccaat gatttctttt agtgaaatac tgtgttcatt
      241 tttgtaataa aaatatgcta atatcaacaa ttcttttaat gataatttat attgttgctt
      301 taactcttga gtgagaaaaa atatgtacgc ttctacattt atgaatttat tgaccttttc
      361 gaaagtttga tagtccacct tacattctcc tactattttc tgatgtccca atactttata
      421 ttatttaaag taata
//
LOCUS       DAHHSC010000444          434 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.455, whole genome shotgun sequence.
ACCESSION   DAHHSC010000444 DAHHSC010000000
VERSION     DAHHSC010000444.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 434)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 434)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..434
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.455"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
BASE COUNT          180 a           68 c           53 g          133 t
ORIGIN      
        1 tgttgatgag aattttgaga tgtagataaa tatcaaacta gtctataggt aaaaatttat
       61 aattaatttg aaaagaatgt ctcaaataat gtttgaatta gcaggtgtca tactaagtaa
      121 tgttaagtaa acattttata ctttatcaga acaaaaaaga catgcatcaa catgtcagtg
      181 aaactaatcc atattatctt aacaacgaaa ttgaatcgaa cctgaaaaac gtatttacaa
      241 atcgcaacta ctctatccaa ggcgaaatcg caaaatctga tccccgaaat aacatatctc
      301 gaaaaaaatt ccataattca atcatcttca ataattaaca atacattact agttccaaat
      361 atactcatac atacatgttt cattgattga ctgcatattg tcataaataa taggggaact
      421 aatcgctaat aata
//
LOCUS       DAHHSC010000445          421 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.456, whole genome shotgun sequence.
ACCESSION   DAHHSC010000445 DAHHSC010000000
VERSION     DAHHSC010000445.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 421)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 421)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..421
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.456"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
BASE COUNT          172 a           42 c           74 g          133 t
ORIGIN      
        1 gtatatatgg agttctttct tttttacaag atgtatatgt ataaatggaa atgtttatta
       61 cattacataa aaacctttag gtgctcttag atggatgtta atcataaaaa aagagatttt
      121 gaaaaatata ggctattata atttagaatt acaaattata aatcttgcca gtggatatgg
      181 gagttcaaca tgtcacttac cccaatttgt ggaatgttaa ataactataa agaggaaagg
      241 agaaaaaatg ataagaatgg ttaaagaaaa agaacgaata gcaaaattaa atttcgaata
      301 gctgtgacga gcttaataat tcaagtactt aattttatca aatcgttctt ttaaaaaggt
      361 aaagggcaaa agacctttac cacattaaat agggaggtgg tatttatgac aagagaatta
      421 a
//
LOCUS       DAHHSC010000446          417 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.459, whole genome shotgun sequence.
ACCESSION   DAHHSC010000446 DAHHSC010000000
VERSION     DAHHSC010000446.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 417)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 417)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..417
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.459"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            complement(<1..>417)
                     /locus_tag="NBI57_002836"
     CDS             complement(<1..>417)
                     /locus_tag="NBI57_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TIGR04141 family sporadically distributed
                     protein"
                     /protein_id="HCD1823714.1"
                     /translation="INNLIDYDFGIDFAERAIKNEDIVNKNVNFFQQNRLKEIVNYRR
                     NSVDYVRPSESYISVQGHPQNPQIFGKTMTCGTSISLRVPNRKQQFIDKISVIIKEIN
                     AIINLPQKISEFPRIVTLKDLNKIEVLDTLLLKKLS"
BASE COUNT          130 a           64 c           50 g          173 t
ORIGIN      
        1 ttcgatagtt tttttagcaa taaagtatct aatacttcta ttttattcaa gtcttttaaa
       61 gttactattc taggaaattc actaattttt tgaggaagat taataatagc gtttatttct
      121 ttgattatca cactaatttt atctatgaat tgctgctttc tattcggtac acgcaatgaa
      181 atacttgtac cacaagtcat cgtttttcca aaaatttgag gattctgtgg atgtccttgg
      241 actgatatat aagattctga aggtctaacg taatctacac tattccttct ataattaaca
      301 atctctttaa gcctgttttg ttgaaaaaaa ttaacatttt tattaactat gtcttcattt
      361 ttgattgctc tttctgcaaa atcaattccg aagtcataat caatcaaatt atttata
//
LOCUS       DAHHSC010000447          416 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.460, whole genome shotgun sequence.
ACCESSION   DAHHSC010000447 DAHHSC010000000
VERSION     DAHHSC010000447.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 416)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 416)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..416
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.460"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            12..>416
                     /locus_tag="NBI57_002837"
     CDS             12..>416
                     /locus_tag="NBI57_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heptaprenyl diphosphate synthase component 1"
                     /protein_id="HCD1823715.1"
                     /translation="METTVSKLERQIEERLKGVSEYESININHRLGKLLDSYDIPDVA
                     KVACLTIDTSMRHLDDITYNHLSKHSILIGDLISAHFYTLLAEINDLSFQNEISKAIV
                     EINELKSSLHHQALNDYEISQAIVKIETLFPYI"
BASE COUNT          160 a           58 c           63 g          135 t
ORIGIN      
        1 gaggtgtaga catggaaaca actgttagca aattggaaag acaaatagaa gaaagattaa
       61 agggtgtatc agaatatgaa tccattaata ttaatcatcg gttaggtaag ttacttgatt
      121 catatgatat tccagatgtt gccaaagtag catgcctcac aattgacaca tcaatgagac
      181 accttgatga cattacatat aatcatttgt ctaaacattc cattttgatt ggagatttaa
      241 ttagtgcaca tttttatacg ctactagcag aaatcaatga tttatcattt caaaatgaaa
      301 ttagtaaagc aattgttgaa attaatgaat tgaaatcttc attacatcat caagctttaa
      361 atgattatga aatttctcaa gcaatagtaa aaattgaaac acttttccca tatatt
//
LOCUS       DAHHSC010000448          400 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.461, whole genome shotgun sequence.
ACCESSION   DAHHSC010000448 DAHHSC010000000
VERSION     DAHHSC010000448.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 400)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 400)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..400
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.461"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            complement(<1..>400)
                     /locus_tag="NBI57_002838"
     CDS             complement(<1..>400)
                     /locus_tag="NBI57_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TIGR04141 family sporadically distributed
                     protein"
                     /protein_id="HCD1823716.1"
                     /translation="ELSNMILLLDDMLDVNIYINSFKNNTLETFDATDPEVDYITEIG
                     DYLLKYDVNSINDVRIEVIDNLGHSNNMLLKTILHAEVEMEDGKKYLLQNGKWGYFNR
                     EFFDLLNDHLNEIEIRYNTLTPTGLVFKEG"
BASE COUNT          129 a           72 c           41 g          158 t
ORIGIN      
        1 tctccttctt taaatactaa acctgtggga gttagtgtgt tatacctaat ttctatctca
       61 ttcaaatgat cattcaaaag gtcaaaaaat tctctattaa aataacccca tttcccattt
      121 tgtaataaat atttctttcc atcttccatt tcaacttctg catgtagtat cgtttttagt
      181 agcatattat ttgaatgccc caaattatct atcacttcaa ttctgacatc atttatagaa
      241 ttaacgtcat attttaataa gtaatctcct atttcagtga tgtaatcaac ttcaggatca
      301 gtcgcatcaa atgtctctaa tgtattattt ttaaaagaat ttatatatat attgacgtcg
      361 agcatatcat ctaacaagag tatcatatta cttagttcta
//
LOCUS       DAHHSC010000449          392 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.463, whole genome shotgun sequence.
ACCESSION   DAHHSC010000449 DAHHSC010000000
VERSION     DAHHSC010000449.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 392)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 392)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..392
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.463"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
BASE COUNT          138 a           65 c           43 g          146 t
ORIGIN      
        1 ccgttaaacg gagaggatta cttcgtatac tgttataact gaaatcttct atatatcatt
       61 tcgtaatatc gctagcttca tctaaaaaat ttaatgctat gatttagccg aattaaaaac
      121 atatatatat ttactatgaa ctaaaatttc actttaaaaa taaaaaacca tgatttcgaa
      181 gtcttcataa actgaagctt ctactctcat ggcaatttat actatgatat tcaattataa
      241 tgcctagtct catagtaaac ggctactaca ttcgcatgta cttcgtttta ctgtccacgc
      301 atggtcgttg acttatttat attataatat aaatatttta tttttcaata cttaatttta
      361 aaaatcccct atgaaaatgt tgatgaacta ca
//
LOCUS       DAHHSC010000450          377 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.465, whole genome shotgun sequence.
ACCESSION   DAHHSC010000450 DAHHSC010000000
VERSION     DAHHSC010000450.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 377)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 377)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..377
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.465"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            <1..>377
                     /locus_tag="NBI57_002839"
     CDS             <1..>377
                     /locus_tag="NBI57_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1823717.1"
                     /translation="ITGMSLFYCLVIFPMTIVVYLRVSQKNYLRSNKIEMIMGTIIAI
                     ISLLLIILQAFNITWGVIPITNFGHQFFFFIGIILVIAGIFYKRLEFSGIGLLFCQKT
                     VDAMIHNPQSAQIFSLIIWILLVV"
BASE COUNT          116 a           50 c           59 g          152 t
ORIGIN      
        1 attactggaa tgagtctatt ttattgttta gtgatttttc caatgactat tgttgtatac
       61 ctaagggtgt cacaaaagaa ttacttgcgt agtaataaaa tagaaatgat tatgggtaca
      121 attatcgcta tcatatcctt gttattaatt atattacaag catttaatat tacttggggc
      181 gttataccaa ttacaaattt tggacatcaa tttttctttt tcattggtat tattttagta
      241 attgccggca tattttataa gcgacttgag ttttcgggaa tcggcttatt attttgtcaa
      301 aaaaccgtcg atgcaatgat tcataatcca caatcagccc agattttttc attaattata
      361 tggatattat tagtagt
//
LOCUS       DAHHSC010000451          371 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.466, whole genome shotgun sequence.
ACCESSION   DAHHSC010000451 DAHHSC010000000
VERSION     DAHHSC010000451.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 371)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 371)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..371
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.466"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            complement(80..313)
                     /locus_tag="NBI57_002840"
     CDS             complement(80..313)
                     /locus_tag="NBI57_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1823718.1"
                     /translation="MVFNDWEINVVYSGEHEVVTNEKSLFVIDEFYDVAIAIYYLDGK
                     LKVAHVNYGSDFTIDVANKVFELNIQNPNVDEH"
BASE COUNT          126 a           64 c           37 g          144 t
ORIGIN      
        1 attatctaat tttcatttgc tttacatgta aaaggctata tatagtatgc tctttatgat
       61 tctaaatgct ttttaatatt taatgctcat caacatttgg attttgaata ttcaattcaa
      121 aaactttatt agctacgtca attgtaaaat cagaaccata gttgacatga gctactttta
      181 attttccatc taaataatag attgcgattg caacatcgta aaattcgtca atgacaaata
      241 aactcttttc gtttgttaca acctcatgct ctcctgagta tacaacgtta atttcccaat
      301 cattaaaaac catttgttaa cctccttgaa catttaaatt gattcaactt aagtttaact
      361 tattcataca a
//
LOCUS       DAHHSC010000452          365 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.467, whole genome shotgun sequence.
ACCESSION   DAHHSC010000452 DAHHSC010000000
VERSION     DAHHSC010000452.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 365)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 365)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..365
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.467"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            57..>365
                     /gene="scb"
                     /locus_tag="NBI57_002841"
     CDS             57..>365
                     /gene="scb"
                     /locus_tag="NBI57_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="complement inhibitor SCIN-B"
                     /protein_id="HCD1823719.1"
                     /translation="MKFKKYILTGTLALLLSSTGIATIEGNKADASSLDKYLTESQFH
                     DKRIAEELRTLLNKSNVYALAAGSLNPYYKRTIMMNEYRAKAALKKNDFVSMADAKVA
                     L"
BASE COUNT          148 a           47 c           62 g          108 t
ORIGIN      
        1 actttcctaa aattgactat ataatcgtta agtatcaatt ttaaggagag tttacaatga
       61 aatttaaaaa atatatatta acaggaacat tagcattact tttatcatca actgggatag
      121 caactataga agggaataaa gcagatgcaa gtagtctgga caaatattta actgaaagtc
      181 agtttcatga taaacgcata gcagaagaat taagaacttt acttaacaaa tcgaatgtat
      241 atgcattagc tgcaggaagc ttaaatccat attataaacg tacgattatg atgaatgaat
      301 atagagctaa agcggcactt aagaaaaatg atttcgtatc aatggctgat gctaaagttg
      361 catta
//
LOCUS       DAHHSC010000453          363 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.468, whole genome shotgun sequence.
ACCESSION   DAHHSC010000453 DAHHSC010000000
VERSION     DAHHSC010000453.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 363)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 363)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..363
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.468"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
BASE COUNT          143 a           46 c           77 g           97 t
ORIGIN      
        1 ttaacacaaa gaaaatggct tgacgaagtg aaaacgtttg aatctgacga aacgagaaaa
       61 gagcgcaacg agtttagtag agctaaatga gtaagcgaga gccgaagaag aggaaagaag
      121 caagcgattg tcacaagtca agaaaggttc ttagcgagga tggtagccaa cttacgttcc
      181 gctagagtag aactggaaat gataatttaa taatgtacac tttcgattgt ctaagtatgt
      241 acaactttaa ttttgtgttt atataaattt aaaatgatat catcgaaaac aaaatattgt
      301 ataaatagag aagagcagta agacggtatc taattgaaaa tgatcttact gctcttttat
      361 ata
//
LOCUS       DAHHSC010000454          356 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.469, whole genome shotgun sequence.
ACCESSION   DAHHSC010000454 DAHHSC010000000
VERSION     DAHHSC010000454.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 356)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 356)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..356
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.469"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            <1..352
                     /locus_tag="NBI57_002842"
     CDS             <1..352
                     /locus_tag="NBI57_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020808033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCD1823720.1"
                     /translation="LNIPVYNFSSGMKQKLNFASMLLVDSNVLLLDEPFNALDHVAQK
                     NFTRILKGLVSKEKIIIFTSHLPNTILELSENIYLLKDGCFTDKRKAQSFNQTTLETW
                     LLESQKEVIPYEEK"
BASE COUNT          138 a           44 c           52 g          122 t
ORIGIN      
        1 tctaaatata cctgtatata atttttcaag tggtatgaag caaaaactaa attttgcttc
       61 tatgttatta gtagattcaa atgttttatt attagatgaa ccatttaatg cccttgacca
      121 tgttgctcaa aaaaatttca cacgaatttt aaagggatta gtttctaaag aaaagataat
      181 aattttcact agtcatttgc ctaatacaat tttagaactc tcagaaaata tttatttgtt
      241 aaaagatgga tgttttacag ataaacgaaa agcacagtca tttaatcaaa ccacgttaga
      301 aacatggttg ttagaaagtc aaaaagaggt gataccttat gaagaaaaat gatttg
//
LOCUS       DAHHSC010000455          355 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.470, whole genome shotgun sequence.
ACCESSION   DAHHSC010000455 DAHHSC010000000
VERSION     DAHHSC010000455.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..355
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.470"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            100..>355
                     /locus_tag="NBI57_002843"
     CDS             100..>355
                     /locus_tag="NBI57_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001628779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coagulase"
                     /protein_id="HCD1823721.1"
                     /translation="MKNKLLVLSLGALCVSQIWESNHASAVVSGEKNPYVSKALELKD
                     KSNKSNSYENYRDSLESLISSLSFADYEKYEEPEYEKAVKK"
BASE COUNT          141 a           27 c           75 g          112 t
ORIGIN      
        1 taattatttt aaaaaggtag atgttatata atttggcttg gcgaaaaaat agggtgtaag
       61 gtaggttgtt aattagggaa aattaaggag aaaatacagt tgaaaaataa attgctagtt
      121 ttatcattgg gagcattatg tgtatcacaa atttgggaaa gtaatcatgc gagtgcagtg
      181 gtttctgggg agaagaatcc atatgtatca aaagctttag aattgaaaga taaaagtaat
      241 aaatccaatt cttacgaaaa ttatagagat agtttagaaa gtttgatttc atcattatct
      301 tttgctgatt atgaaaaata tgaagagcca gaatatgaaa aggctgtaaa aaaat
//
LOCUS       DAHHSC010000456          349 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.472, whole genome shotgun sequence.
ACCESSION   DAHHSC010000456 DAHHSC010000000
VERSION     DAHHSC010000456.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 349)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 349)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..349
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.472"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            118..200
                     /locus_tag="NBI57_002844"
     tRNA            118..200
                     /locus_tag="NBI57_002844"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:151..153,aa:Leu,seq:gag)
     gene            222..295
                     /locus_tag="NBI57_002845"
     tRNA            222..295
                     /locus_tag="NBI57_002845"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:254..256,aa:Gly,seq:tcc)
BASE COUNT          120 a           60 c           69 g          100 t
ORIGIN      
        1 tgataacaca aattaattta ttgtaaaaaa acgattaaaa gttattgaca aatattttaa
       61 aacataatat aataaataac gttctgaaaa acgaagatgt tttaaatgcc tatatccgcg
      121 gtcgtggcgg aatggcagac gcgctaggtt gagggcctag tgggagaaat cccgtggagg
      181 ttcaagtcct ctcggccgca tcaaaattct taattaaata agcgggtgta gtttaatggc
      241 aaaacctcag ccttccaagc tgatgttgtg ggttcgattc ccatcacccg ctccatagat
      301 aattttaatg aatattgaaa actgaatgac aatatgtcaa cgttaattc
//
LOCUS       DAHHSC010000457          348 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.473, whole genome shotgun sequence.
ACCESSION   DAHHSC010000457 DAHHSC010000000
VERSION     DAHHSC010000457.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 348)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 348)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..348
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.473"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            <1..195
                     /locus_tag="NBI57_002846"
     CDS             <1..195
                     /locus_tag="NBI57_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01741 family protein"
                     /protein_id="HCD1823722.1"
                     /translation="DFTKEGELKVSFDYIDWINTEFDQLGRQNYYMYKKFGVIPEMEY
                     EMEEVKEIEQYIKEQEEAEQ"
     gene            206..>348
                     /locus_tag="NBI57_002847"
     CDS             206..>348
                     /locus_tag="NBI57_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF600 family protein"
                     /protein_id="HCD1823723.1"
                     /translation="MTFEEKISKLYNEIANEISSMIPVEWEKVYTMAYIDDGGGEVFF
                     NYT"
BASE COUNT          148 a           28 c           75 g           97 t
ORIGIN      
        1 gattttacaa aagaaggtga attaaaagtt tcatttgatt atatagattg gatcaataca
       61 gagtttgatc aattgggccg tcaaaattat tatatgtaca aaaaatttgg ggttatacca
      121 gaaatggaat atgaaatgga agaagttaaa gaaatcgaac aatatattaa agagcaagaa
      181 gaagctgaac aatagagatg ataacatgac tttcgaagag aaaataagca aattatataa
      241 tgagattgcg aatgagatta gcagtatgat accggtagag tgggaaaaag tatatacaat
      301 ggcttatata gatgatggag gaggtgaagt attctttaat tatactaa
//
LOCUS       DAHHSC010000458          308 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.477, whole genome shotgun sequence.
ACCESSION   DAHHSC010000458 DAHHSC010000000
VERSION     DAHHSC010000458.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 308)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 308)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..308
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.477"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            complement(<1..241)
                     /locus_tag="NBI57_002848"
     CDS             complement(<1..241)
                     /locus_tag="NBI57_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500656.1"
                     /note="secreted protein that specifically inhibits the
                     activation of neutrophils and monocytes by binding to the
                     formylated peptide receptor and the C5a receptor; blocks
                     neutrophil migration towards the infection site and
                     hinders the establishment of the initial defense against
                     the infection; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein"
                     /protein_id="HCD1823724.1"
                     /translation="MKKKLATTVLALSFLTAGISTHHHSAKAFTFEPFPTNEEIESNK
                     KMLEKEKAYKESFKNSGLPTTLGKLDERLRNYLEKG"
BASE COUNT           87 a           48 c           39 g          134 t
ORIGIN      
        1 tgcctttctc taaataattt ctcaaacgtt catctaattt tcctagcgtt gtaggaagac
       61 cactattttt aaatgattct ttataagctt tttctttctc taacattttc ttatttgatt
      121 ctatttcttc atttgtagga aacggttcaa aagtaaaagc tttcgctgaa tgatggtgtg
      181 tactgattcc tgccgttaaa aaacttaatg ctaaaactgt tgttgctaat ttctttttca
      241 taatgatgtt aattctcctt aaattctata tttaaattta tagttagttt tgcgaaattc
      301 ctaaaatg
//
LOCUS       DAHHSC010000459          301 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.478, whole genome shotgun sequence.
ACCESSION   DAHHSC010000459 DAHHSC010000000
VERSION     DAHHSC010000459.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 301)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 301)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..301
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.478"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            complement(<1..>301)
                     /locus_tag="NBI57_002849"
     CDS             complement(<1..>301)
                     /locus_tag="NBI57_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001624188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1823725.1"
                     /translation="SNKEEVNLHKPFFYISYSIWFFLGCLIYFSQMAYNQLANAVIFI
                     LILNTITRDSFSAGTDFFTKSLRFDSDRKSIGLYRMSNTDFKQIYDSKLSLIRFIG"
BASE COUNT          124 a           45 c           37 g           95 t
ORIGIN      
        1 ccaataaagc gaattagaga taatttagaa tcgtaaattt gtttaaaatc agtgtttgac
       61 atcctataca aaccgatact ttttctatct gaatcaaaac gtaatgattt agtaaaaaaa
      121 tccgtccccg cagaaaaact atcacgagtt atagtgttta atatcaaaat aaaaataact
      181 gcattagcta actgattata tgccatctgt gaaaaataga ttaaacaccc taaaaagaac
      241 catattgaat aactaatata gaagaaaggt ttatgtaaat taacttcttc tttgttactt
      301 a
//
LOCUS       DAHHSC010000460          297 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.479, whole genome shotgun sequence.
ACCESSION   DAHHSC010000460 DAHHSC010000000
VERSION     DAHHSC010000460.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 297)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 297)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..297
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.479"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            <1..>297
                     /locus_tag="NBI57_002850"
     CDS             <1..>297
                     /locus_tag="NBI57_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000226989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 family protein"
                     /protein_id="HCD1823726.1"
                     /translation="LVLFIPLLNWLTPKRYIKISQEELESLNTFKPAKNNAFWPALGS
                     SVLFSVTFRKYMPLFNVRLEKTIVIAIFFVVFLGILFFYLNLNRRLALSVFTMNK"
BASE COUNT          100 a           36 c           44 g          117 t
ORIGIN      
        1 ttagttttat tcattccatt gcttaattgg ttgacaccaa aaagatacat caagatcagt
       61 caagaagaat tagaaagttt aaatactttc aaacctgcta aaaacaatgc attttggcca
      121 gcattaggta gttcagtttt atttagtgtg acttttagaa agtatatgcc tttatttaat
      181 gttagattgg aaaaaacaat agtaatagcc atattttttg ttgtatttct tggtatttta
      241 tttttctacc taaatttaaa tcgtaggcta gcattaagtg tttttactat gaataaa
//
LOCUS       DAHHSC010000461          286 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.481, whole genome shotgun sequence.
ACCESSION   DAHHSC010000461 DAHHSC010000000
VERSION     DAHHSC010000461.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 286)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 286)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..286
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.481"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            80..256
                     /locus_tag="NBI57_002851"
     CDS             80..256
                     /locus_tag="NBI57_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkvS family protein"
                     /protein_id="HCD1823727.1"
                     /translation="MTVAEVGNIVEFMDGLRGRVEKINDNSVIVDLTIMENFNDLDLP
                     EKTVINHKRYKIVE"
BASE COUNT          105 a           22 c           55 g          104 t
ORIGIN      
        1 atttttgatt gttaatagtt tatttgttaa gtgaatttta gtacaatata atttagttat
       61 gatttaagga ggacttaaaa tgactgttgc agaagtgggt aacattgttg agtttatgga
      121 tggattaaga ggtcgtgttg aaaaaatcaa cgataactct gttattgttg acttaacaat
      181 tatggaaaat tttaatgacc ttgatttacc ggaaaaaact gttatcaatc ataaacgata
      241 taagattgtt gaataagaag gtaagttata atgaataaaa tctcga
//
LOCUS       DAHHSC010000462          271 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.484, whole genome shotgun sequence.
ACCESSION   DAHHSC010000462 DAHHSC010000000
VERSION     DAHHSC010000462.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 271)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 271)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..271
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.484"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            complement(<1..>271)
                     /locus_tag="NBI57_002852"
     CDS             complement(<1..>271)
                     /locus_tag="NBI57_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001624188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1823728.1"
                     /translation="LLINPMDKQLLYRSSLREVDIFNLIYASDVLKKFVGYFNLLIVG
                     MAISLYHISPFIFNLKVFCALIYFSTISYVIQIIFINIKVISTKKL"
BASE COUNT          115 a           36 c           29 g           91 t
ORIGIN      
        1 ataatttttt tgtgcttatg actttaatat ttataaaaat aatttgaatt acataagaga
       61 ttgttgaaaa gtatattagt gcacaaaaaa ccttgaggtt aaatataaaa ggactaatat
      121 gatataaact aatagccata ccaactatca acaaattaaa atatccaaca aactttttta
      181 aaacgtctga agcatatatc aagttaaaaa tgtcaacttc tctcaaacta gatctatata
      241 acagctgttt atccattgga ttgatcaata a
//
LOCUS       DAHHSC010000463          270 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.485, whole genome shotgun sequence.
ACCESSION   DAHHSC010000463 DAHHSC010000000
VERSION     DAHHSC010000463.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 270)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 270)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..270
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.485"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            complement(<1..>270)
                     /locus_tag="NBI57_002853"
     CDS             complement(<1..>270)
                     /locus_tag="NBI57_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan bridge formation glycyltransferase
                     FemA/FemB family protein"
                     /protein_id="HCD1823729.1"
                     /translation="VLDLAAALFICTDDEVYYLSSGSNPKYNQYMGAYHLQWHMIKYA
                     KSHNINRYNFYGITGVFSNEADDFGVQQFKKGFNAHVEELIGDFIK"
BASE COUNT           98 a           51 c           28 g           93 t
ORIGIN      
        1 tttgatgaaa tcaccaatta attcttcaac atgtgcatta aaaccctttt taaattgttg
       61 aacaccaaaa tcatccgcct cattactaaa gacgcctgtt attccataaa aattatacct
      121 attaatatta tgtgattttg catattttat catatgccat tgtagatgat atgcacccat
      181 atactgatta tatttcggat ttgatccact tgatagataa taaacttcat catcagtaca
      241 tataaataaa gcagcagcta aatccaaaac
//
LOCUS       DAHHSC010000464          265 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.488, whole genome shotgun sequence.
ACCESSION   DAHHSC010000464 DAHHSC010000000
VERSION     DAHHSC010000464.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 265)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 265)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..265
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.488"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            72..>265
                     /locus_tag="NBI57_002854"
     CDS             72..>265
                     /locus_tag="NBI57_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001253430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1823730.1"
                     /translation="MRRLLSLLLASTMILVACGNANNENKKKEDEKKSEVKKEATKNN
                     DKPKNEKKNQDINKNNNEQV"
BASE COUNT          134 a           28 c           43 g           60 t
ORIGIN      
        1 aaatctaaga atttagatat tgtttaattt taaaactatc gattcacata taaataaaag
       61 gagataacag catgaggaga ttactcagtc tattactagc tagtacaatg attttagtcg
      121 catgtgggaa tgctaacaat gaaaataaga aaaaggaaga cgaaaaaaaa tcagaagtaa
      181 aaaaagaagc tacgaaaaat aatgataaac caaagaacga aaagaagaat caagatataa
      241 ataaaaacaa taatgagcaa gttca
//
LOCUS       DAHHSC010000465          261 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.489, whole genome shotgun sequence.
ACCESSION   DAHHSC010000465 DAHHSC010000000
VERSION     DAHHSC010000465.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 261)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 261)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..261
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.489"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            <1..>261
                     /locus_tag="NBI57_002855"
     CDS             <1..>261
                     /locus_tag="NBI57_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000613248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP synthase"
                     /protein_id="HCD1823731.1"
                     /translation="LNIMTHINSQNNNFKRNPFINEEQLKSLLAIKKVDAHMSYDSNI
                     TSKALAEINKTQKRLVTKIDTLYNQSKKENEYVRLGEKISYTK"
BASE COUNT          127 a           34 c           35 g           65 t
ORIGIN      
        1 ttaaatatca tgacgcatat caatagtcaa aataataatt ttaaacgtaa tccatttata
       61 aatgaagaac aattgaaatc actactcgcg ataaagaaag tagatgcaca tatgagttat
      121 gattcaaata ttacttcaaa agcattggca gaaataaaca aaacgcaaaa aagattagtt
      181 acaaagatag acacactcta taatcagtca aaaaaagaaa atgaatatgt acggttagga
      241 gaaaaaatat cgtatacaaa a
//
LOCUS       DAHHSC010000466          260 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.491, whole genome shotgun sequence.
ACCESSION   DAHHSC010000466 DAHHSC010000000
VERSION     DAHHSC010000466.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 260)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 260)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..260
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.491"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            complement(6..>260)
                     /locus_tag="NBI57_002856"
     rRNA            complement(6..>260)
                     /locus_tag="NBI57_002856"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT           63 a           73 c           60 g           64 t
ORIGIN      
        1 cttagaaagg aggtgatcca gccgcacctt ccgatacggc taccttgtta cgacttcacc
       61 ccaatcattt gtcccacctt cgacggctag ctcctaaaag gttactccac cggcttcggg
      121 tgttacaaac tctcgtggtg tgacgggcgg tgtgtacaag acccgggaac gtattcaccg
      181 tagcatgctg atctacgatt actagcgatt ccagcttcat gtagtcgagt tgcagactac
      241 aatccgaact gagaacaact
//
LOCUS       DAHHSC010000467          253 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.492, whole genome shotgun sequence.
ACCESSION   DAHHSC010000467 DAHHSC010000000
VERSION     DAHHSC010000467.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 253)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.492"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            48..>253
                     /gene="seu"
                     /locus_tag="NBI57_002857"
     CDS             48..>253
                     /gene="seu"
                     /locus_tag="NBI57_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_031927818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphylococcal enterotoxin type C1/U"
                     /protein_id="HCD1823732.1"
                     /translation="MKLFAFIFICVKSCSLLFMLNGNPKPEQLNKASEFTGLMDNMRY
                     LYDDKHVSETNIKSQEKFLQHDLL"
BASE COUNT           96 a           31 c           38 g           88 t
ORIGIN      
        1 ctgtaataca tcaaatttat aaaaaattaa aatatggagt tgttgtaatg aagttatttg
       61 cctttatctt catatgtgtt aagtcttgca gcttactatt tatgttaaat ggcaatccta
      121 aaccagaaca attgaataaa gcgagtgaat tcactggtct aatggataat atgaggtatt
      181 tgtatgatga taaacacgta tcagaaacaa acattaaatc ccaagaaaag tttttacaac
      241 atgatttatt att
//
LOCUS       DAHHSC010000468          247 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.495, whole genome shotgun sequence.
ACCESSION   DAHHSC010000468 DAHHSC010000000
VERSION     DAHHSC010000468.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 247)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 247)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..247
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.495"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            <1..>247
                     /locus_tag="NBI57_002858"
     rRNA            <1..>247
                     /locus_tag="NBI57_002858"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT           77 a           47 c           69 g           54 t
ORIGIN      
        1 taagggcgca cggtggatgc cttggcacta gaagccgatg aaggacgtta ctaacgacga
       61 tatgctttgg ggagctgtaa gtaagctttg atccagagat ttccgaatgg ggaaacccag
      121 catgagttat gtcatgttat cgatatgtga atacatagca tatcagaagg cacacccgga
      181 gaactgaaac atcttagtac ccggaggaag agaaagaaaa ttcgattccc ttagtagcgg
      241 cgagcga
//
LOCUS       DAHHSC010000469          233 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.499, whole genome shotgun sequence.
ACCESSION   DAHHSC010000469 DAHHSC010000000
VERSION     DAHHSC010000469.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 233)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 233)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..233
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.499"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
BASE COUNT           78 a           47 c           28 g           80 t
ORIGIN      
        1 ttagacataa aatctaaaaa cagcagtaag atgatttatg attaaaaact atcttactgc
       61 tgttcacttt ttataatact tctgaatgtc ttcacttata cttctagtca cagatttaaa
      121 taatcaaaag tgcacattat taaaatatca atttcacact caatgcggct catcgcattc
      181 atttcttgtc tagcaacgtt ctactctagc ggaacgtaag ttggctacca tcc
//
LOCUS       DAHHSC010000470          230 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.500, whole genome shotgun sequence.
ACCESSION   DAHHSC010000470 DAHHSC010000000
VERSION     DAHHSC010000470.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 230)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 230)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..230
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.500"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
BASE COUNT           73 a           38 c           26 g           93 t
ORIGIN      
        1 gattttcgcg cgattactac gatgatttca aacgattcct ctgattattt cacgcgattt
       61 ccacaacatt tttcgtattt cagaacgatt aagactacat tatcctggct taatattaat
      121 taattttaat tatatctata aatattttca gtgaaaaact atttctttaa ggcatctaat
      181 gctttaaaaa acaacgatac attgtactca atattgttat gctttgtttt
//
LOCUS       DAHHSC010000471          216 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.505, whole genome shotgun sequence.
ACCESSION   DAHHSC010000471 DAHHSC010000000
VERSION     DAHHSC010000471.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 216)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 216)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..216
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.505"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
BASE COUNT           76 a           26 c           34 g           80 t
ORIGIN      
        1 ttttatagat ggtggggata ttaagttaaa aacttaaaat catcttatca taaataacaa
       61 tcttaagtta gcattcacga taatagtcat tgttaacatt agcatataag gtcatgtcac
      121 gttgaaacag aggttcctcg gcatttttga aaataatcat tagcttgctc actatataat
      181 ttgatgaata tatttcgtaa aagtgggtat ttattt
//
LOCUS       DAHHSC010000472          210 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.denovo.506, whole genome shotgun sequence.
ACCESSION   DAHHSC010000472 DAHHSC010000000
VERSION     DAHHSC010000472.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 210)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 210)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..210
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.denovo.506"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            1..129
                     /locus_tag="NBI57_002859"
                     /pseudo
     CDS             1..129
                     /locus_tag="NBI57_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499947.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT           80 a           28 c           32 g           70 t
ORIGIN      
        1 tacttccaag ttcaaaatcg cacattgaaa tgatgtgtga aaatgtttaa actacggtca
       61 ttttgtgaaa ataaagataa taactattca ttttacaata gtgaaaagtc agtatatgac
      121 aacaattaat attgcggtaa ggccttgtgt tacagtattc tatatttaag tactgcaatc
      181 agaattaaca gaatgccatt aactgattat
//
LOCUS       DAHHSC010000473        10340 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.guided.081, whole genome shotgun sequence.
ACCESSION   DAHHSC010000473 DAHHSC010000000
VERSION     DAHHSC010000473.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 10340)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10340)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10340
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.guided.081"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            82..1068
                     /locus_tag="NBI57_002860"
     CDS             82..1068
                     /locus_tag="NBI57_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001549960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactam sensor/signal transducer MecR1"
                     /protein_id="HCD1823733.1"
                     /translation="MLSSFLMLSIISSLLTICVIFLVRMLYIKYTQNIMSHKIWLLVL
                     VSTLIPLIPFYKISNFTFSKDMMNRNVSDTTSSVSHMLDGQQSSVTKDLAINVNQFET
                     SNITYMILLIWVFGSLLCLFYMIKAFRQIDVIKSSSLESSYLNERLKVCQSKMQFYKK
                     HITISYSSNIDNPMVFGLVKSQIVLPTVVVETMNDKEIEYIILHELSHVKSHDLIFNQ
                     LYVVFKMIFWFNPALYISKTMMDNDCEKVCDRNVLKILNRHEHIRYGESILKCSILKS
                     QHINNVAAQYLLGFNSNIKERVKYIALYDSMPKPNRNKRIVAYIVCSISSFT"
     gene            1040..1300
                     /locus_tag="NBI57_002861"
     CDS             1040..1300
                     /locus_tag="NBI57_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001549961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3387 domain-containing protein"
                     /protein_id="HCD1823734.1"
                     /translation="MYVVYRASHETAKEALGDKELRAIAHELTKTVKDNMSVDWSKRD
                     SAKAKMRVQVRRLLKKYGYPPDLQKMAVEQVVEQAELMASQQ"
     gene            complement(1291..2898)
                     /locus_tag="NBI57_002862"
                     /pseudo
     CDS             complement(1291..2898)
                     /locus_tag="NBI57_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011274780.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182 family transposase"
     gene            complement(2950..3456)
                     /locus_tag="NBI57_002863"
     CDS             complement(2950..3456)
                     /locus_tag="NBI57_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001092170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1643 domain-containing protein"
                     /protein_id="HCD1823735.1"
                     /translation="MNTIKSTIHTEAIFSDDEQHRYLLKKTWDEKKAACTVITMYPHL
                     DGVLSLDLTTVLILNQLANSERYGAVYLVNLFSNIRTPENLKHIKNPYDEHTDIHLMK
                     AISESDTVILAYGAYAKRPVVIDRVEQVMEMLKPHKKKVKKLINPVTNEIMHPLNPKA
                     RQKWILKS"
     gene            complement(3471..3782)
                     /locus_tag="NBI57_002864"
     CDS             complement(3471..3782)
                     /locus_tag="NBI57_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002505017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HCD1823736.1"
                     /translation="MKINRYITRGINESIPLDLQILLWHMVEKKDNQPHTDYLHIFKL
                     QEDENILSITHEQEQPTYKLEYHYTNYEKNQNALPKKVYVIREDDVDAFYYVMLLPEE
                     Y"
     gene            complement(3869..4219)
                     /locus_tag="NBI57_002865"
     CDS             complement(3869..4219)
                     /locus_tag="NBI57_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002502216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1823737.1"
                     /translation="MKTITQELKQYITHLFQLSNNEAWECEALEEAAENILPERFIND
                     SPLVHLTLETYTYYNDELHELSIYPFLMYANNQLISVGYLDHFDMDFLYLTDTQNIII
                     DERHLLKQGGQDHE"
     gene            complement(4741..6369)
                     /locus_tag="NBI57_002866"
     CDS             complement(4741..6369)
                     /locus_tag="NBI57_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001186610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCD1823738.1"
                     /translation="MQQLKTKRVGIYVRVSTEMQSTEGYSIDGQINQIKEYCDFHHFE
                     VKDIYADRGISGKSMNRPELQRMLKDAKEGNIDCVMVYKTNRLARNTSDLLKIVEDLH
                     KQNVEFFSLSERMEVNTSSGKLMLQILASFSEFERNNIVENVFMGQTRRAQEGYYQGN
                     LPLGYDKIPDSKHELMINQHEANIVKYIFECYAKGHGYRKIANALNHKGYVTKKGKPF
                     SISSITYILANPFYIGKIQFAKYKDWSEKRRKGLNDKPVIAEGKHSPIINQDLWDKVQ
                     MRKKQVSQKPQVHGKGTNLLTGIIHCPQCGAPMAASNTTNTLKDGTKKRIRYYSCSNF
                     RNKGSKVCSANSVRADVIEDYVMKQILEIVKSDKVIQRVVTHVNQENQVDGAALHHDI
                     AYKQQQYDEVQIKINNLIKTIEDNPDLTSVIRPSIQKYEKQLNDITNQINQLKNQQNE
                     DKTLFDAKEISKLLQHIFHDIKHIEKSRLKALYLSVIDRIDIKKDGNHKKQFYVTLKL
                     NNEIIKQLFNNKQLDEVHLSTSSLFLPQTLYLTI"
     gene            complement(6391..7740)
                     /locus_tag="NBI57_002867"
     CDS             complement(6391..7740)
                     /locus_tag="NBI57_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000815654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCD1823739.1"
                     /translation="MKQVIGYLRQSTMKQQSLAAQKQAIEAIAEKHHIQHINFYSDKQ
                     SGRKDNRSGYRQITQLIQQGQCDILCCYRLNRLHRNLKNALKLIKLCQTYHVHILSVH
                     DGYFDMDQAFDRLKLNIFISLAELESDNIGEQVRNGLQEKAKQGRLITTHAPFGYEYH
                     NGTFIINQNESPTVKAVFNYYIKGHGYKKIAQLLEEDNTYINRQPYQVRNIIINPNYC
                     GRVNNQYGQFDNMFPSIVSTSIYEQAQRLRLQKQTKQTPSDNQLKQKIKCPCCNATLT
                     NMTIRKKNHTLRYYVCPKNMNASRFVCDFKGINAQTLEDKVLEVCRDFYQNQRIYTKI
                     KSAIDKRIKRQRNIEKHHTLTQEQLIEKLAQGIIDAETFREQTQSLRQQPQRTTSING
                     HQIQHIIQNIIQKRFTLNILYPYIETIHITKDKNLIGIYFKNEPLNIVNQTMQSSIA"
     gene            complement(7974..9761)
                     /locus_tag="NBI57_002868"
     CDS             complement(7974..9761)
                     /locus_tag="NBI57_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001573627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF927 domain-containing protein"
                     /protein_id="HCD1823740.1"
                     /translation="MSYTLFEIGPFALDSFGWKETIPPKKDGDSEKVVRMASPIIVNA
                     RFSDPITGVEKLIITNNNGKKDIFESDILTTRNLPSLIKYGYSINEKYIRSLSYALQL
                     MRDRLPLSELYEGVGILETPFGYLISLDKVLKSIQFNQSSPSYPIVDSAYDLTPKGTF
                     DNWFNMYIDEVKGHLLLELAVIFGISALVTSFLKHKHEIEFAGILFSFTGQSSTGKST
                     AAALAVSVAGNPTKGNETLFRSWNATRNALEGYLSNNYGIPIVFDELSSTTLRDTTGL
                     LYSIAEGQGRQRSNVHGEVKTPKNWGTSVISTSEYSIFNDSAQNDGLRVRTIEINEQF
                     TTNATNADNIKKAVALNYGHVLPLVAKYLINREDEVIQWFYKEVDWFEAKLKDETNNT
                     GIRMFKRYAVITTSAKILGRVLSTDIDIANIRDYFIDYHTHTVSERSLADKAIDVIIQ
                     FVAQNRGKFSDEGALKNMFENYGLISLKDDHIEVKIIANVFKQMLNNHQFQDVNNVVN
                     ALRDKGFILADRGRQTTKRSVKDNSGKKQSLVFYHLKLDVEFASILGLTKDKSLLQNW
                     TPSNDNKAAKELFKSANEGIGPSGVHEDF"
     gene            complement(9761..10051)
                     /locus_tag="NBI57_002869"
     CDS             complement(9761..10051)
                     /locus_tag="NBI57_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001036438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1823741.1"
                     /translation="MNKKSKQQEKLYNFIIAKSFQQPVGSTFTYGELRKKYNVVCSTN
                     DQREVGRRFAYWIKYTPGLPFKIVGTKNGSLLYQKIGINPCNNSTPSKGGDC"
     gene            10190..>10340
                     /locus_tag="NBI57_002870"
     CDS             10190..>10340
                     /locus_tag="NBI57_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000613248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP synthase"
                     /protein_id="HCD1823742.1"
                     /translation="MIPTLEEIIDKYGNLVDYLKFDVTVEVYEDLLLLRSLSDLNEHQ
                     KMDRVS"
BASE COUNT         3062 a         1625 c         1775 g         3878 t
ORIGIN      
        1 agactacatt tgtagtatat tacaaatgta gtatttatgt caaaataatg ttataatttt
       61 tgtgatatgg aggtgtagaa ggtgttatca tcttttttaa tgttaagtat aatcagttca
      121 ttgctcacga tatgtgtaat ttttttagtg agaatgctct atataaaata tactcaaaat
      181 attatgtcac ataagatttg gttattagtg ctcgtctcca cgttaattcc attaatacca
      241 ttttacaaaa tatcgaattt tacattttca aaagatatga tgaatcgaaa tgtatctgac
      301 acgacttctt cggttagtca tatgttagat ggtcaacaat catctgttac gaaagactta
      361 gcaattaatg ttaatcagtt tgagacctca aatataacgt atatgattct tttgatatgg
      421 gtatttggta gtttgttgtg cttattttat atgattaagg cattccgaca aattgatgtt
      481 attaaaagtt cgtcattgga atcgtcatat cttaatgaac gacttaaagt atgtcaaagt
      541 aagatgcagt tctacaaaaa gcatataaca attagttata gttcaaacat tgataatccg
      601 atggtatttg gtttagtgaa atcccaaatt gtactaccaa ctgtcgtagt cgaaaccatg
      661 aatgacaaag aaattgaata tattattcta catgaactat cacatgtgaa aagtcatgac
      721 ttaatattca accagcttta tgttgttttt aaaatgatat tctggtttaa tcctgcacta
      781 tatataagta aaacaatgat ggacaatgac tgtgaaaaag tatgtgatag aaacgtttta
      841 aaaattttga atcgccatga acatatacgt tatggtgaat cgatattaaa atgctctatt
      901 ttaaaatctc agcacataaa taatgtggca gcacaatatt tactaggttt taattcaaat
      961 attaaagaac gtgttaagta tattgcactt tatgattcaa tgcctaaacc taatcgaaac
     1021 aagcgtattg ttgcgtatat tgtatgtagt atatcgagct tcacatgaaa cagctaaaga
     1081 agctttgggc gataaagagt taagagccat tgcacatgag ttaactaaaa cagttaagga
     1141 taacatgagt gttgattggt ctaaacgaga cagtgctaaa gctaaaatga gagttcaagt
     1201 tagacgccta ttaaagaaat atggctatcc accagatctt caaaaaatgg ctgtggaaca
     1261 agttgtagag caagcagaat taatggcaag tcagcaataa aaaaataaat cataatgagt
     1321 ccgggacata aagttcttgg ataagtgaaa aaagacaatt tctattgaaa taatatagaa
     1381 attgtctttt ttataaattt tttgattatt ttcagctcgt tgagctacta cttttcttat
     1441 attaagtgcc attaatacaa aaccaagttc tcttttgact ttattgagtc ctcggacaga
     1501 catccgagtg aaacccaaaa tagccttcat aaatccaaaa acaggttcca catcaatttt
     1561 tctttgactg tagatatttt ttgtttctgg ttctgaaagc tttttgttaa tttgggattt
     1621 aaaatattcc cagttataat tcttcattat ttttttgttt gtttttgaat tgaagttcat
     1681 acattgattt ttcagaggac attctgaaca atcatcacat tcatataatt tgaagtctcg
     1741 cttataacca tacttatcat gacgataggc atatctttta aaacctagcc gtttattatt
     1801 cggacaaatg aattcgtcat taatttcgtc atagttccaa ttttgagtat taaagatgtc
     1861 acttttatat tttttagttt tatcttttat aaacattcca tatgttatga gtggcgttcg
     1921 attaaagtca tctataattg ccttataatt tgattcacta ccataacctg catcagctac
     1981 aatatattca ggtaaatgac cgtaggtctc ttgaattgaa tttaaaaatg gaatcatcgt
     2041 tctagtatcc gttggatttt gatacacatt ataagataaa acaaattggg aatttgttgc
     2101 tatttgtaaa ttataccctg gcttaagttg tccatttttc atgtgatctt ctttcattct
     2161 cataaatgtc gcatcataat ctgtcttaga ataactattt ctatccttta aaatagattt
     2221 ttgaaattcg tatcgatact ttcgctcaaa ataatcattg atttgctttt tgtatttttt
     2281 gattttagtt cttttgagac gtatttgttt tcttgtttta gtacattttt cattgttgat
     2341 atgttggttt aaatcttcga tttctttatc taagtgacta ccaatcaaat ctatttcttc
     2401 ttttgttaat tcattatcat gatcttcttt aatttccggt atgattttat tggttaccaa
     2461 ttcatggtag agggctttag aatcctcatt catctttgat tcatggtttt gaatactctt
     2521 tttccataca aatgtatatc gattggcatt tgcttcaatt tttgtaccat caataaaaat
     2581 agctttatca tctataagat tttgttttac acactgactg taaaattgaa taaataaaga
     2641 ttctaataaa gcatctactt ttggatttac tctaaattga ttaattgttt tataagaagg
     2701 tttttgattt tgtgatagcc acatcattcg gatgctatca ttaagcattt tttctatttt
     2761 acgacctgag aatacagatt gtgtgtaggc atatagaatc acttttaaca tcattttagg
     2821 atggtacgaa gttgcaccac ggtgatgtct gaattcgtcg aattcattgt caggaattgt
     2881 ttcaacaata tcatttacag taaaacgatg ttgatttgtt ttgtttccat attgacctcc
     2941 atgtatttgc tatgatttca aaatccattt ttgacgtgcc ttagggttga gtggatgcat
     3001 aatttcattt gttactggat tgatgagctt ttttactttc tttttatgag gttttaacat
     3061 ttccatcact tgttcgacac ggtcgataac aactggtcgc ttcgcatagg caccataagc
     3121 aagaatcact gtgtcacttt cactaatcgc tttcatcaaa tgaatatcag tgtgctcatc
     3181 gtatggattt ttgatatgtt tgaggttttc gggtgttcta atattagaga atagatttac
     3241 aagatataca gcaccgtatc gttctgaatt ggctaattgg ttgaggataa gaacagttgt
     3301 gagatcgagt gataatacac cgtctaaatg aggatacatc gttatcactg tgcatgcagc
     3361 tttcttttca tcccatgttt tcttgagtaa atagcggtgc tgttcatcat cgctaaatat
     3421 ggcttctgtg tgtatcgtac ttttgattgt attcatcatc gtcacttcct ttagtattct
     3481 tctggtaaaa gcatcacata ataaaaagcg tccacgtcat cttcacgaat gacgtagact
     3541 ttcttaggta atgcattttg atttttttca tagtttgtat agtgatattc caatttgtat
     3601 gtgggttgtt cttgttcatg tgtgattgag agtatattct catcttcttg taatttaaaa
     3661 atgtgtaggt aatctgtatg aggctggtta tctttttttt ctaccatgtg ccaaagtaag
     3721 atttgaaggt ctagtggaat actttcatta attcctcttg tgatgtatcg attgattttc
     3781 atgctatttc cctcccttct gcttttcttt catgatgtcg atgatttcgt tgataactgt
     3841 gacggataat tgagcaacta tgatccaatt attcatggtc ctgacctcct tgttttagta
     3901 aatgacgttc atcaataatg atattttgag tatctgtaag gtacagaaag tccatgtcaa
     3961 aatggtctaa gtatccgaca ctgatgagtt ggttattggc atacattaga aatggataga
     4021 tacttagctc atgtagttca tcattatagt aggtataagt ctcgagtgtg agatgtacca
     4081 gtggagaatc attaataaaa cgttccggta gaatattttc tgctgcttcc tccagcgctt
     4141 cacattccca agcttcgtta ttagatagtt ggaataggtg ggttatatat tgtttgagtt
     4201 cttgagtgat ggttttcata ttattgcctc ctagatagtg taatagtgat gtagttcata
     4261 tacatcattg agataatata tatttgattt gtcatttatt acgaatcccg gtgggaataa
     4321 gagaaaattc catataaaaa cccgctacaa acgttggtat gccaaggaaa tcctgaaatt
     4381 ccgcctattt tgacaaacaa tcaactcatt atttataagt attgatgata gggttgtgtc
     4441 tctgcttcct tatatatatt atttatttat aaaaagtaac gggattttgg gattgtgctt
     4501 gcacaatcct tctgtttctt cgaatctgca aatcccaatc atttcccgat aaaaaatcat
     4561 tgtgggatgt tctttagcaa tttcaatata agcattgtat agttatgaaa aaattacgac
     4621 aataactgtt tcattagata agtgttattg aaattgataa agagcaattc ttgaaaatag
     4681 ttagataaaa taagcgaaag aatatagtga aaattattgt tataacaatg attctattag
     4741 ctaaatagta agatatagtg tttggggcaa aaataaagac gaagtgctga gatgcacttc
     4801 gtcgagttgt ttattattga aaagttgttt aatgatttcg ttattaagtt tgagtgtgac
     4861 atagaattgt tttttatgat taccatcttt tttaatatca atgcgatcaa tcactgatag
     4921 atacaatgct ttgagtcgag atttttctat gtgcttaata tcatgaaaga tgtgttgtaa
     4981 tagtttactg atttctttgg catcaaataa agtcttatct tcattttgtt gatttttgag
     5041 ttggttgatt tgattcgtaa tgtcattgag ttgcttttca tatttttgaa tacttggtct
     5101 gattactgat gttaagtccg gattatcctc gatggtttta atcaagttat ttattttgat
     5161 ttgtacttca tcatattgtt gttgcttata agcaatatcg tgatgaagtg cagcgccatc
     5221 aacttgattt tcttgattga cgtgtgttac tacgcgttga atgactttat cacttttgac
     5281 tatttcaagt atttgcttca tcacataatc ttcaatcaca tcagctctta cactgtttgc
     5341 cgaacatact ttggaaccct tgttccgaaa attactacat gaatagtaac gaatacgttt
     5401 cttagtcccg tctttaagag tattcgtggt attgcttgct gccataggtg cgccacattg
     5461 gggacagtga ataatgcctg taagcagatt cgttcctttg ccatggactt ggggtttttg
     5521 actgacttgt ttcttacgca tttgtacttt atcccataaa tcttgattaa taatggggga
     5581 atgcttacct tcagctatca ctggtttatc attcagccct ttacgacgtt tttcactcca
     5641 atctttgtat ttcgcaaatt gaattttgcc gatatagaaa gggttagcta agatgtatgt
     5701 gattgaacta atactgaaag gtttcccctt tttagtgaca tatcctttgt gattcaatgc
     5761 attggcaatt ttacgatagc catgtccttt ggcatagcac tcgaatatat attttacaat
     5821 attagcttca tgttggttaa tcattagctc gtgtttacta tctggtattt tgtcataacc
     5881 tagtggtaaa ttgccttgat aatagccttc ttgggcacgt ctcgtttgac ccataaatac
     5941 gttctcgaca atgttattac gttcgaattc tgagaaactc gcaagtatct gtaacatgag
     6001 tttaccagaa gaagtattga cttccatacg ctctgacaaa ctgaaaaatt cgacattttg
     6061 tttgtgtaaa tcttcgacaa ttttgagaag atcagatgta ttacgagcta atcggtttgt
     6121 tttgtatacc ataacacagt cgatattgcc ttcttttgca tctttcaaca tacgttggag
     6181 ctcaggtctg ttcatagatt tacctgaaat accacggtca gcgtatatat ctttaacttc
     6241 aaaatgatgg aagtcacagt attctttgat ttgattgatt tgtccgtcga tactataacc
     6301 ttctgtgctt tgcatttctg ttgatacacg tacatagata ccgacacgtt ttgttttaag
     6361 ttgttgcatt atgttacatc ctttcttcat ttatgcaatc gatgattgca tggtttgatt
     6421 gacaatattg agtggttcat ttttgaaata gattcctata agatttttat ctttcgtaat
     6481 gtgaatggtt tcaatatagg ggtacaatat gtttaacgtg aaacgttttt gaataatatt
     6541 ttgaatgatg tgttgtattt gatgcccatt gatagatgta gtgcgttgcg gttgttgacg
     6601 taatgattgt gtttgctctc tgaacgtttc tgcatcaatg atgccttgtg ccaacttttc
     6661 tatcagttgt tcttgagtca atgtgtgatg tttttctatg tttctttgtc ttttgatgcg
     6721 tttgtcaatc gcacttttaa tttttgtgta gatgcgttga ttttgataaa agtctcggca
     6781 cacttctaat actttatctt caagtgtttg tgcattgatg cctttaaaat cacagacaaa
     6841 gcgtgaagca ttcatgtttt taggacagac gtagtaacgt aatgtatgat tcttttttct
     6901 aatggtcata tttgtaagtg ttgcattaca acatgggcat ttgatttttt gtttgagttg
     6961 attatccgaa ggtgtctgtt tggtttgttt ttgcaatcga agtctctgcg cttgctcata
     7021 tatacttgtg gaaacaatag aaggaaacat attgtcgaat tggccatatt gattgttgac
     7081 acgaccacaa taattaggat tgatgataat gttacgaact tgatagggtt gtcgattgat
     7141 atatgtgtta tcttcttcta ataactgtgc aattttctta taaccatgac ctttaatgta
     7201 ataattgaat acagccttta ccgttggtga ctcattttga ttgatgatga atgttccgtt
     7261 gtgatattcg taaccaaaag gtgcatgtgt tgtaatcaat cgaccttgct ttgctttttc
     7321 ttgaagccca tttctgactt gttctccaat gttatccgat tcaagttcgg ctaaactgat
     7381 gaagatatta agcttgagtc ggtcgaaagc ttgatccata tcaaagtaac catcatgtac
     7441 gcttaagata tgaacatggt acgtttgaca taatttgatg agttttaatg catttttcag
     7501 attacgatgc aacctattaa gacgataaca gcataatatg tcacactgtc cttgttgaat
     7561 taattgtgtt atttgtcgat acccactacg attatctttg cgacctgatt gtttgtcgct
     7621 ataaaagttg atatgttgaa tatgatgttt ttcggctatt gcttcgatag cttgtttctg
     7681 tgctgcaaga gattgttgtt tcatcgtact ttgacgtaaa tagcctatga cttgtttcat
     7741 atcggctcct cctttcacag tgataatata tatttatgga tgaattgata tataagccca
     7801 acatcaatga gatgttgggc gtccatatta gtcatttgtt tgattgattt cttcaattac
     7861 caaatcggct aatatctcga taagttcatc catgtttttc actccgttat ttgttctatc
     7921 ttcaatacgt cgattattca gtttgatgct tcacagttgt atgataaaga caattagaaa
     7981 tcttcgtgaa ctcctgaagg gcctatccct tcattagcgg atttaaaaag ttctttcgca
     8041 gctttgttat catttgacgg tgtccaattt tgaagtaacg acttatcttt agttaatccg
     8101 aggatagatg caaactctac atctaatttt agatggtaaa atacaagtga ttgtttttta
     8161 ccgctattat ctttgacact tcttttagtt gtttggcgtc cacggtcagc taatatgaaa
     8221 cctttatctc ttaaggcgtt gacaacatta ttaacatctt gaaattgatg attgtttagc
     8281 atctgtttaa aaacgttcgc aattattttt acttcgatat ggtcatcttt taatgagatt
     8341 aatccatagt tctcaaacat atttttcaaa gcaccttcat ctgaaaactt acctctgttt
     8401 tgtgccacaa attgaatgat gacatcaata gctttatcag ctaatgagcg ttcagagact
     8461 gtatgagtat gataatcaat aaagtagtct cttatattag cgatatcaat atctgtagat
     8521 aaaacacgac ctaatatttt cgcagatgtt gtaatgactg cataacgctt aaacatacga
     8581 atacctgtat tgtttgtttc atctttcaat ttagcttcaa accaatctac ttccttgtaa
     8641 aaccattgaa taacttcatc ttcacgattt ataagatatt tagctactaa cggtaaaaca
     8701 tgaccatagt ttagtgctac agctttttta atattgtcag cattggtcgc atttgtagtg
     8761 aattgttcat taatctcgat ggttcttaca cgtaatccat cgttttgagc tgaatcatta
     8821 aaaatactgt attctgacgt tgaaatgaca gaagtacccc aattcttagg cgttttaact
     8881 tctccatgaa cgtttgaacg ttgacgacct tgaccttcag cgatggaata taacaaaccc
     8941 gtggtatctc taagtgtcgt agatgaaagc tcatcaaata ctataggaat gccataattg
     9001 ttactcaaat aaccttcaag tgcgtttcgt gtggcattcc aacttctaaa aagagtttca
     9061 ttacctttgg tagggttacc agcgactgat acagctaaag cagctgcggt tgacttaccg
     9121 gttgaggatt gacctgtaaa actaaagaga attcctgcaa attcgatttc atgtttatgc
     9181 ttcagaaaac ttgtcactaa ggcagaaatc ccaaatatga ctgccaattc taaaagaaga
     9241 tgacctttaa cctcgtcaat atacatgtta aaccaattat caaatgtacc tttaggagtt
     9301 aagtcataag cactatctac aatagggtat gaaggtgagg actgattaaa ttgaatagat
     9361 ttgagaactt tatctagcga tatcaagtag ccaaaaggtg tctctagtat acctacaccc
     9421 tcatataatt cagaaagtgg taatctatct cgcatcaatt gcaaggcata actcaatgat
     9481 ctaatatact tctcgttaat actgtagcca tatttaatta aagaaggtaa gtttcgtgtt
     9541 gttaagatat cagactcaaa aatgtctttt ttaccattgt tgttagtaat aatcaacttt
     9601 tcaacaccag taattggatc agaaaatctt gcattaacaa taatagggct agccattcta
     9661 accacttttt cactgtcacc atctttttta ggtggtatcg tttctttcca accgaaggaa
     9721 tctaaagcaa atgggccaat ttcaaacaat gtgtaactca ttagcaatca cctcctttcg
     9781 aaggcgtgct attattgcat gggtttatcc ctattttctg atataataaa ctgccatttt
     9841 ttgttcctac aattttaaat ggtaatcctg gcgtgtactt aatccagtaa gcaaaacgac
     9901 gtcctacttc acgttgatca ttcgtgctac aaaccacatt atatttcttt cttaattcac
     9961 cataagtgaa cgtacttcca actggttgtt gaaaagattt agcaattata aaattataaa
    10021 gcttttcttg ctgcttagat ttcttattca ttttaaaatt cctccaataa tttttaagga
    10081 gtcaatcaat atcatatagg ttaaactatc ttcaattgat tgctataata agcataaatg
    10141 aagcattatg attagacgcg gaacaaaaaa tggggagggg gggtatatat tgatacctac
    10201 gctagaagaa attattgata agtatggaaa cttagttgat tatttgaaat ttgatgtaac
    10261 agttgaggtt tatgaagatt tgttgttatt aagaagttta agcgatttaa atgaacatca
    10321 aaagatggat agagtttcat
//
LOCUS       DAHHSC010000474         3773 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.guided.214, whole genome shotgun sequence.
ACCESSION   DAHHSC010000474 DAHHSC010000000
VERSION     DAHHSC010000474.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3773)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3773)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3773
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.guided.214"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            322..3396
                     /gene="mupA"
                     /locus_tag="NBI57_002871"
     CDS             322..3396
                     /gene="mupA"
                     /locus_tag="NBI57_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mupirocin-resistant isoleucine--tRNA ligase
                     MupA"
                     /protein_id="HCD1823743.1"
                     /translation="MTKKYLNTQNEISAFWNTQKIFKKSIDNRKGQESFVFYDGPPTA
                     NGLPHAGHVLGRVIKDLVARLKTMQGFYVERKAGWDTHGLPVELEVEKKIGIKGKQDI
                     EKYGIENFINECKKSVFNYEKEWRDFSKDLGYWVDMDSPYITLENNYIESVWNILSTF
                     HKKGLLYKGHKVTPYCTHDQTALSSHEVAQGYKNVKDLSAVVKFQLTNSKDTYFLSWT
                     TTPWTLPANVALAINKDLNYSKIRVENEYYILATDLINSIITEKYEIIDTFSGSNLIN
                     LKYIPPFESDGLVNAYYVVDGEFVTNSEGTGIVHIAPAHGEDDYQLVLERDLDFLNVI
                     TREGVYNDRFPELVGNKAKNSDIEIIKLLSKKQLLYKKQKYEHNYPHCWRCGNPLIYY
                     AMEGWFIKTTNFKNEIINNNNNIEWFPSHIKEGRMGNFLENMVDWNIGRNRYWGTPLN
                     VWICNDCNHEYAPSSIKDLQNNSINKIDEDIELHRPYVDNITLSCPKCNGKMSRVEEV
                     IDVWFDSGSMPFAQHHYPFDNQKIFNQHFPADFIAEGVDQTRGWFYSLLVISTILKGK
                     SSYKRALSLGHILDSNGKKMSKSKGNVINPTELINKYGADSLRWALISDSAPWNNKRF
                     SENIVAQTKSKFIDTLDNIYKFYNMYNKIDHYNPNNEITKSRNTLDNWALSRLNTLIK
                     ESNIYVNNYDFTSAARLINEYTNTISNWYIRRSRGRFWEQGISNDKKDAYNTLYEILT
                     TLSRLVAPFVPFISEKIHYNLTGKSVHLQDYPQYKESFINQALEDEMHTVIKIVELSR
                     QARKNADLKIKQPLSKMVIKPNSQLNLSFLPNYYSIIKDELNIKNIELTDNINDYITY
                     ELKLNFSSVGPKLGNKTKNIQTLIDSLSEYDKKSLIESNNFKSLSSDAELTKDDFIIK
                     TLPKDSYQLSEDNDCVILLDKNLSPELIREGHARELIRLIQQLRKKKNLPINQRIDIY
                     IGVTGELLESIKTNKNMFKENLLIKNIHLNVIDEYENTIHFNNKEIKISLLY"
     gene            3466..3732
                     /locus_tag="NBI57_002872"
                     /pseudo
     CDS             3466..3732
                     /locus_tag="NBI57_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000282188.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
BASE COUNT         1499 a          472 c          587 g         1215 t
ORIGIN      
        1 taaaaatttt gaagaacaaa tgattttcaa cttcaaagat tacgaagtta atcaaactat
       61 taaaataaat aaaatttata atagtatagt ttcaacatgg gatgtttata aaaataacga
      121 atatagcaaa gacctttcaa gagaattaaa atttaaatta tatactacta atgatataat
      181 ttgcagtagt ttgtattata actttacttt atccaatata tctttctatg gagacatgaa
      241 aggaaatgaa tttcatgaaa attcaccaag actaatagta aaaataacga aataagtgat
      301 actctaggag gctgaaaagt tttgacaaag aaatatttaa acacccagaa tgaaatatca
      361 gcattttgga atactcaaaa gatatttaaa aaatcaattg acaatagaaa aggacaggaa
      421 agttttgttt tttatgacgg ccccccaact gcaaatggcc ttcctcatgc tggccatgtt
      481 cttggaagag taatcaagga tttagttgca agattaaaaa ctatgcaagg tttttatgta
      541 gaaagaaaag caggatggga tacccatggc ttaccagttg aattagaggt tgaaaaaaaa
      601 attggaatta aaggaaaaca agacattgaa aagtatggaa tagaaaattt tataaatgaa
      661 tgtaaaaaaa gtgtatttaa ttatgaaaaa gaatggcggg atttttctaa agatttagga
      721 tactgggttg acatggactc cccctatata actcttgaga ataattatat tgaaagtgta
      781 tggaatatat tatctacatt ccataaaaaa ggactattat ataagggaca taaggtgact
      841 ccttattgta cacatgatca aaccgcttta agttctcatg aagtagcgca aggctataaa
      901 aacgttaaag atttatcagc tgttgttaaa tttcaactta caaatagtaa agatacttat
      961 ttcttaagtt ggactaccac tccctggact ttgcctgcaa atgtagcatt agctataaat
     1021 aaagatctta attattcaaa aattcgggta gaaaatgagt attatatctt agctacagat
     1081 ctaattaatt ctataataac tgaaaaatac gaaattattg ataccttttc aggaagtaat
     1141 ttaattaatt taaaatacat tcctcctttt gaaagcgacg gtttagttaa tgcatattac
     1201 gttgttgatg gagaatttgt tactaactca gaaggaactg gtattgttca tatagcacca
     1261 gctcatgggg aagatgacta ccaattggtt ttagagcgtg atttggattt cttaaatgtt
     1321 ataacaagag aaggagtata taatgatagg ttccctgaat tagttggtaa taaagctaaa
     1381 aatagtgata tagaaatcat aaaattatta tccaaaaaac aacttttata taaaaaacaa
     1441 aaatatgagc ataattatcc tcattgttgg agatgtggta atcctttgat atattatgcg
     1501 atggagggtt ggtttattaa aacaactaat tttaagaatg aaattattaa caataataat
     1561 aatatagagt ggtttccttc tcatattaag gaagggagaa tgggaaattt cttagaaaat
     1621 atggttgatt ggaacattgg tagaaataga tattggggaa caccattaaa tgtatggatt
     1681 tgcaatgatt gtaatcacga atacgcacca agtagtatta aggatttaca aaataattcc
     1741 atcaataaaa ttgatgaaga tattgagttg catagacctt atgttgataa tatcactctt
     1801 agttgcccta agtgtaatgg gaaaatgtct cgagtagaag aagtaatcga tgtttggttt
     1861 gatagcggct ctatgccgtt tgctcagcat cattatcctt ttgataacca gaaaattttt
     1921 aatcaacact ttccagctga ttttattgca gaaggagttg atcaaacgag aggctggttt
     1981 tacagtttac tagtaatttc tactattcta aaaggaaaat cttcttataa acgtgcttta
     2041 tctttaggac atattctaga cagtaatggt aaaaaaatgt ctaaaagtaa aggaaacgtt
     2101 attaatccaa ctgaattaat taataagtac ggagccgatt ctttaagatg ggccttaatt
     2161 tcggatagtg ctccatggaa taacaaaaga ttctcagaaa atatagtagc tcagaccaaa
     2221 tcgaaattta tagatacgct tgataatatt tataaatttt ataatatgta taataaaata
     2281 gatcactata atcctaataa tgaaattaca aaaagtagaa atacattaga taattgggct
     2341 ctttctcgct taaacacctt aataaaagaa agtaatattt atgtaaataa ttacgatttc
     2401 acttccgcag ccagattaat taacgaatat accaatacaa taagtaattg gtatattcgg
     2461 agatcgagag gacgattttg ggaacaagga atttctaacg ataaaaaaga tgcgtacaat
     2521 acgctttatg aaattttaac aactttatca agactagtgg ctccatttgt tccatttata
     2581 tctgaaaaaa tccattataa tttgactgga aaaagtgtgc atttacaaga ttatccacaa
     2641 tataaagaaa gttttattaa tcaagcattg gaagatgaaa tgcataccgt tataaaaatt
     2701 gtagaattat ctagacaggc tcgcaaaaat gcagatttaa aaattaagca acctttatcg
     2761 aaaatggtga ttaaacctaa tagtcaatta aacttaagtt ttttacctaa ttactattca
     2821 ataataaaag acgaattaaa tataaaaaac attgaattaa ctgataatat taatgactat
     2881 attacctatg agcttaaatt gaatttttct tctgtgggac caaaactagg gaacaaaacg
     2941 aaaaatattc aaacattgat agactcccta tcagagtatg ataaaaaaag tttaattgag
     3001 tctaataact tcaaaagttt atcttctgat gctgagttaa ctaaggatga ttttataatt
     3061 aaaaccttac ctaaggatag ttatcaactc agtgaagata atgactgcgt tatattatta
     3121 gataaaaatt tatctcctga attaattcgc gaaggacatg ctagagagct cattagatta
     3181 attcaacaat taagaaaaaa gaaaaattta ccaataaatc aacgtattga tatttatatc
     3241 ggtgtaactg gggaattatt agaatcaata aaaaccaata aaaatatgtt taaagaaaat
     3301 ttgctgatta aaaatataca cttaaatgtt atagatgaat atgaaaatac tattcatttt
     3361 aataataaag aaataaaaat ttccttatta tattaaacaa acatggccac tctattttag
     3421 tagagtggcc atgagttatc aaagccaagc aaaaggtgat aaactatgta taattttgat
     3481 tattctaaat taccaattaa aaatattcaa aaaatatttc ctattgcagg gggatatgtg
     3541 aatctatctt tttcagttga tgcttccaac aaaaaatatt ttttaaagct acaacctaat
     3601 actaaatcaa atttttttga ttatgagtta tccagtttaa aagaattaac tgataaaaat
     3661 attcctgttc ctcaaattat taataaagga gaattagaca ataattcttt tttactgtta
     3721 gagttcattg aaaaggttct gttgcaaagt aaaaaaatat agctaaccac taa
//
LOCUS       DAHHSC010000475         3616 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.guided.218, whole genome shotgun sequence.
ACCESSION   DAHHSC010000475 DAHHSC010000000
VERSION     DAHHSC010000475.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3616)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3616)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3616
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.guided.218"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            <1..1867
                     /gene="mec"
                     /locus_tag="NBI57_002873"
     CDS             <1..1867
                     /gene="mec"
                     /locus_tag="NBI57_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001801873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PBP2a family beta-lactam-resistant peptidoglycan
                     transpeptidase"
                     /protein_id="HCD1823744.1"
                     /translation="DSSYISKSDNGEVEMTERPIKIYNSLGVKDINIQDRKIKKVSKN
                     KKRVDAQYKIKTNYGNIDRNVQFNFVKEDGMWKLDWDHSVIIPGMQKDQSIHIENLKS
                     ERGKILDRNNVELANTGTAYEIGIVPKNVSKKDYKAIAKELSISEDYIKQQMDQNWVQ
                     DDTFVPLKTVKKMDEYLSDFAKKFHLTTNETESRNYPLGKATSHLLGYVGPINSEELK
                     QKEYKGYKDDAVIGKKGLEKLYDKKLQHEDGYRVTIVDDNSNTIAHTLIEKKKKDGKD
                     IQLTIDAKVQKSIYNNMKNDYGSGTAIHPQTGELLALVSTPSYDVYPFMYGMSNEEYN
                     KLTEDKKEPLLNKFQITTSPGSTQKILTAMIGLNNKTLDDKTSYKIDGKGWQKDKSWG
                     GYNVTRYEVVNGNIDLKQAIESSDNIFFARVALELGSKKFEKGMKKLGVGEDIPSDYP
                     FYNAQISNKNLDNEILLADSGYGQGEILINPVQILSIYSALENNGNINAPHLLKDTKN
                     KVWKKNIISKENINLLTDGMQQVVNKTHKEDIYRSYANLIGKSGTAELKMKQGETGRQ
                     IGWFISYDKDNPNMMMAINVKDVQDKGMASYNAKISGKVYDELYENGNKKYDIDE"
     gene            complement(1913..2341)
                     /locus_tag="NBI57_002874"
     CDS             complement(1913..2341)
                     /locus_tag="NBI57_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000872606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HCD1823745.1"
                     /translation="MKYDDFIVGETFKTKSLHITEEEIIQFATTFDPQYMHIDKEKAE
                     QSRFKGIIASGMHTLSISFKLWVEEGKYGEEVVAGTQMNNVKFIKPVYPGNTLYVIAE
                     ITNKKSIKKENGLVTVSLSTYNENEEIVFKGEVTALINNS"
     gene            complement(2438..3181)
                     /locus_tag="NBI57_002875"
     CDS             complement(2438..3181)
                     /locus_tag="NBI57_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001830227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphoryl diester phosphodiesterase"
                     /protein_id="HCD1823746.1"
                     /translation="MLTVYGHRGLPSKAPENTIASFKAASEVEGINWLELDVAITKDE
                     QLIIIHDDYLERTTNMSGEITELNYDEIKDASAGSWFGEKFKDEHLPTFDDVVKIANE
                     YNMNLNVELKGITGPNGLALSKSMVKQVEEQLTNLNQNQEVLISSFNVVLVKLAEEIM
                     PQYNRAVIFHTTSFREDWRTLLDYCNAKIVNTEDAKLTKAKVKMVKEAGYELNVWTVN
                     KPARANQLANWGVDGIFTDNADKMVHLSQ"
BASE COUNT         1285 a          592 c          549 g         1190 t
ORIGIN      
        1 agatagcagt tatatttcta aaagcgataa tggtgaagta gaaatgactg aacgtccgat
       61 aaaaatatat aatagtttag gcgttaaaga tataaacatt caggatcgta aaataaaaaa
      121 agtatctaaa aataaaaaac gagtagatgc tcaatataaa attaaaacaa actacggtaa
      181 cattgatcgc aacgttcaat ttaattttgt taaagaagat ggtatgtgga agttagattg
      241 ggatcatagc gtcattattc caggaatgca gaaagaccaa agcatacata ttgaaaattt
      301 aaaatcagaa cgtggtaaaa ttttagaccg aaacaatgtg gaattggcca atacaggaac
      361 agcatatgag ataggcatcg ttccaaagaa tgtatctaaa aaagattata aagcaatcgc
      421 taaagaacta agtatttctg aagactatat caaacaacaa atggatcaaa attgggtaca
      481 agatgatacc ttcgttccac ttaaaaccgt taaaaaaatg gatgaatatt taagtgattt
      541 cgcaaaaaaa tttcatctta caactaatga aacagaaagt cgtaactatc ctctaggaaa
      601 agcgacttca catctattag gttatgttgg tcccattaac tctgaagaat taaaacaaaa
      661 agaatataaa ggctataaag atgatgcagt tattggtaaa aagggactcg aaaaacttta
      721 cgataaaaag ctccaacatg aagatggcta tcgtgtcaca atcgttgacg ataatagcaa
      781 tacaatcgca catacattaa tagagaaaaa gaaaaaagat ggcaaagata ttcaactaac
      841 tattgatgct aaagttcaaa agagtattta taacaacatg aaaaatgatt atggctcagg
      901 tactgctatc caccctcaaa caggtgaatt attagcactt gtaagcacac cttcatatga
      961 cgtctatcca tttatgtatg gcatgagtaa cgaagaatat aataaattaa ccgaagataa
     1021 aaaagaacct ctgctcaaca agttccagat tacaacttca ccaggttcaa ctcaaaaaat
     1081 attaacagca atgattgggt taaataacaa aacattagac gataaaacaa gttataaaat
     1141 cgatggtaaa ggttggcaaa aagataaatc ttggggtggt tacaacgtta caagatatga
     1201 agtggtaaat ggtaatatcg acttaaaaca agcaatagaa tcatcagata acattttctt
     1261 tgctagagta gcactcgaat taggcagtaa gaaatttgaa aaaggcatga aaaaactagg
     1321 tgttggtgaa gatataccaa gtgattatcc attttataat gctcaaattt caaacaaaaa
     1381 tttagataat gaaatattat tagctgattc aggttacgga caaggtgaaa tactgattaa
     1441 cccagtacag atcctttcaa tctatagcgc attagaaaat aatggcaata ttaacgcacc
     1501 tcacttatta aaagacacga aaaacaaagt ttggaagaaa aatattattt ccaaagaaaa
     1561 tatcaatcta ttaactgatg gtatgcaaca agtcgtaaat aaaacacata aagaagatat
     1621 ttatagatct tatgcaaact taattggcaa atccggtact gcagaactca aaatgaaaca
     1681 aggagaaact ggcagacaaa ttgggtggtt tatatcatat gataaagata atccaaacat
     1741 gatgatggct attaatgtta aagatgtaca agataaagga atggctagct acaatgccaa
     1801 aatctcaggt aaagtgtatg atgagctata tgagaacggt aataaaaaat acgatataga
     1861 tgaataacaa aacagtgaag caatccgtaa cgatggttgc ttcactgttt tattatgaat
     1921 tattaataag tgctgttact tctcccttaa atacaatttc ttcattttca ttgtatgttg
     1981 aaagtgacac tgtaacgagt ccattttctt tttttatgga tttcttattt gtaatttcag
     2041 cgataacgta caatgtatta cctgggtata caggtttaat aaatttaacg ttattcattt
     2101 gtgttcctgc tacaacttct tctccgtatt taccttcttc tacccataat ttaaatgata
     2161 ttgaaagtgt atgcatgcca gatgcaatga tacctttaaa tctactttgt tctgcttttt
     2221 ctttatctat atgcatatat tgaggatcaa aagttgttgc aaattggata atttcttctt
     2281 ctgtaatatg aaggcttttt gttttgaatg tttctcctac tataaaatca tcgtatttca
     2341 tatatgtctc tctttcttat tcaaattaat tttttagtat gtaacatgtt aaaggtaagt
     2401 ctaccgtcac tgaaacgtaa gactcacctc taactttcta ttgagacaaa tgcaccattt
     2461 tatctgcatt gtctgtaaag ataccatcaa ctccccaatt agcaagttgg tttgcacgtg
     2521 ctggtttgtt tacagtccat acgttcaatt cataacccgc ttcttttacc atttttactt
     2581 ttgctttagt aagtttggca tcttcagtgt ttactatttt agcattacag taatctaaaa
     2641 gtgttctcca gtcttcacga aacgaagttg tatggaatat aactgctctg ttatattgtg
     2701 gcatgatttc ttctgcaagt ttaacaagca caacattaaa gcttgaaatg agcacttctt
     2761 gattctgatt taagtttgtt aattgttctt ccacttgctt aaccatactt ttagaaagtg
     2821 ctagtccatt cggtccagta atacctttta attctacatt taaattcata ttatattcat
     2881 ttgctatttt tactacatca tcgaaagttg gcaaatgttc atctttgaat ttttcaccaa
     2941 accaagatcc tgcagaagca tctttaattt catcataatt caattcagtt atttccccgg
     3001 acatatttgt agtccgttct aaataatcat catgaatgat aatcagttgt tcatcttttg
     3061 taattgcaac atctaactcc aaccagttta taccttctac ttctgaagca gctttaaatg
     3121 atgcaattgt attttccgga gctttactag gtaatcctct atgtccatat acagttagca
     3181 tattacctct ccttgcattt ttattttttt aattaacgta actgtattat cacattaatc
     3241 gcacttttat ttccattaaa aagagatgaa tatcataaat aaagaagtcg atagattcgt
     3301 attgattatg gagttaatct acgtctcatc tcatttttaa aaaatcattt atgtcccaag
     3361 ctccattttg taatcaagtc tagtttttct gtacccctta tctgcaattt tacttaggat
     3421 tgcttttaac ttacccctta tcagcaattt tactgagaac tgcttttaac gcacctctta
     3481 tctgcaattt tgcctagaac tgcttttaac gtacctctta tctgcaattt tactgagaac
     3541 tgcttttaac ttacccctta tcagcaattt tgcatggaat tgcttttaac gtacctctta
     3601 tctgcaattt tactta
//
LOCUS       DAHHSC010000476         3228 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.guided.229, whole genome shotgun sequence.
ACCESSION   DAHHSC010000476 DAHHSC010000000
VERSION     DAHHSC010000476.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3228)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3228)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3228
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.guided.229"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            complement(30..785)
                     /locus_tag="NBI57_002876"
     CDS             complement(30..785)
                     /locus_tag="NBI57_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCD1823747.1"
                     /translation="MEVVYVTQTQPKFLTIYNTLYKEIQIGKYPSGHALPTEKELCAR
                     FDVSRMTLRQAIKLLAEDGIVESTRGKGHFVIPQTNAQHASSMDQFKHPLDQILLARV
                     SMSSINYRVDLESEYTNHLFANHPSAVIAMERYYQKKDNHSKQADAFCFTFIPLNVID
                     TFKVNTQNEDDMKTFVEQTIYSNAYQSDLKMSITKAPRFKNHSHVFDGDTHCWLIIET
                     LYAQTPYPIMINKWYIPQEISELTLTRIRQSDY"
     gene            1021..1728
                     /gene="deoD"
                     /locus_tag="NBI57_002877"
     CDS             1021..1728
                     /gene="deoD"
                     /locus_tag="NBI57_002877"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HCD1823748.1"
                     /translation="MKSTPHIKPMNDVEIAETVLLPGDPLRAKFIAETYLDDVEQFNT
                     VRNMFGFTGTYKGKKVSVMGSGMGMPSIGIYSYELIHTFGCKKLIRVGSCGAMQENID
                     LYDVIIAQGASTDSNYVQQYQLPGHFAPIASYQLLEKAVETARDKGVRHHVGNVLSSD
                     IFYNADTTASERWMRMGILGVEMESAALYMNAIYAGVEALGVFTVSDHLIHETSTTPE
                     ERERAFTDMIEIALSLV"
     gene            1735..3087
                     /gene="tet(38)"
                     /locus_tag="NBI57_002878"
     CDS             1735..3087
                     /gene="tet(38)"
                     /locus_tag="NBI57_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001100300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(38)"
                     /protein_id="HCD1823749.1"
                     /translation="MNVEYSKIKKAVPILLFLFVFSLVIDNSFKLISVAIADDLNISV
                     TTVSWQATLAGLVIGIGAVVYASLSDAISIRTLFIYGVILIIIGSIIGYIFQHQFPLL
                     LVGRIIQTAGLAAAETLYVIYVAKYLSKEDQKTYLGLSTSSYSLSLVIGTLSGGFIST
                     YLHWTNMFLIALIVVFTLPFLFKLLPKENNTNKAHLDFVGLILVATIATTVMLFITNF
                     NWLYMIGALIAIIVFALYIKNAQRPLVNKSFFQNKRYASFLFIVFVMYAIQLGYIFTF
                     PFIMEQIYHLQLDTTSLLLVPGYIVAVIVGALSGKIGEYLNSKQAIITAIILIALSLI
                     LPAFAVGNHISIFVISMIFFAGSFALMYAPLLNEAIKTIDLNMTGVAIGFYNLIINVA
                     VSVGIAIAAALIDFKALNFPGNDALSSHFGIILIILGLMSIVGLVLFVILNRWTQSEK
                     "
BASE COUNT         1024 a          488 c          576 g         1140 t
ORIGIN      
        1 atcaaaaaat ttcttatatt ttaataattt taataatccg attgtcttat acgtgtcagt
       61 gttaattcag atatttcctg tggaatatac cacttattaa tcataattgg ataaggtgtt
      121 tgtgcgtaca gtgtttcaat aatcagccaa caatgtgtat caccatcaaa cacgtgacta
      181 tgatttttga agcggggcgc tttggtaata gacattttta aatctgattg atatgcattg
      241 ctataaatcg tttgctcaac gaatgtcttc atgtcgtctt cgttttgtgt attcacttta
      301 aatgtgtcaa tgacatttaa cggtataaag gtaaagcaaa atgcatcagc ttgcttagaa
      361 tgattgtcct ttttttgata atagcgttcc attgcaatga cggcagaagg atggtttgca
      421 aacaaatgat ttgtatattc actttctaaa tcaacacgat aattaattga tgacatagat
      481 acgcgagcta gcaatatttg atcaagtgga tgcttaaatt gatccatact tgaagcgtgt
      541 tgggcatttg tttgtggaat aacaaagtgt cccttccctc ttgtactctc tacgatgcca
      601 tcttcggcta acaattttat agcttggcgc aaagtcatac gactgacatc aaagcgcgca
      661 caaagttcct tttcagtagg taatgcatgg ccactcggat attttcctat ttgaatttct
      721 ttatataacg tattataaat cgttaaaaat tttggttgtg tttgcgtcac gtagacaacc
      781 tccataaagt tacttaatca ctctcatcat acaataattt ttactcgaat tggaaaaatt
      841 ataaaaatta aatatagata ggctttgaaa attagtttta tacaaggtta gtagctgtaa
      901 ctgtaaaatg ttcttaatat tgtcaaaatg taatgcttga aagcgctttt aaaaaatatt
      961 attatataca tgattagaca aatagacaaa tcactataca aatattggga ggaatatttt
     1021 atgaaatcaa caccacacat taaaccaatg aatgacgtcg aaattgcaga aacggttcta
     1081 ttgccaggag atccgttaag agctaagttc attgcagaaa cttatttgga tgatgtggaa
     1141 cagttcaata cagtgcgaaa catgtttggt tttaccggaa catataaagg taaaaaagtt
     1201 tctgtcatgg gttcaggtat gggtatgcca tctattggca tttactctta tgaattaatt
     1261 catacatttg gttgtaaaaa attaattcgc gttggctctt gtggcgcgat gcaagaaaac
     1321 attgatttat atgatgtgat tattgcacaa ggtgcctcta ctgattcaaa ttacgttcaa
     1381 caatatcaat taccaggtca ttttgcgcca attgcttctt atcaattatt agaaaaagca
     1441 gttgaaacag cacgtgacaa aggtgtacgt catcatgtag gtaatgtgtt atcaagtgat
     1501 attttctata acgcggatac aacagcgagt gaacgttgga tgcgtatggg tattttaggt
     1561 gtagaaatgg aatcagctgc attatacatg aatgcaattt acgctggtgt cgaagcatta
     1621 ggtgtgttca cagtgagcga tcatttaatt catgaaacgt caacaacacc tgaggaaagg
     1681 gaacgtgcat ttacagatat gattgaaatt gcactgtcat tggtgtagat gattatgaat
     1741 gttgaatatt ctaaaataaa gaaagcagta cctattttat tattcttatt tgtattcagt
     1801 ttggttatag acaactcatt taaattgatt tctgtagcca ttgctgatga cttaaacata
     1861 tctgtaacga cagtaagttg gcaagcgaca ttagccggtt tagtaattgg tattggcgct
     1921 gtagtatacg cttcattatc tgatgccatt agtatacgca cactatttat ttatggcgtg
     1981 atattaatca ttatcggatc aattattggt tacattttcc aacatcaatt cccattactt
     2041 ttagttggac gtattattca aactgccggt ttagctgctg cagagacatt atatgtgata
     2101 tatgttgcaa agtatctttc taaagaggac cagaagactt accttggctt aagtacgagc
     2161 agttattcct tgtcattagt tatcggtaca ttatcaggtg gatttatttc tacgtattta
     2221 cactggacaa atatgttttt aattgcatta atcgtagtat ttacgttgcc attcctattt
     2281 aaattattac caaaagaaaa taatacgaat aaagctcatt tagattttgt tggcttaatt
     2341 ctagtggcaa ctattgctac aacagtcatg ctgtttatta cgaactttaa ttggttatat
     2401 atgattggtg ccttaattgc gattatcgtt tttgcgctat atattaaaaa tgcgcaacgt
     2461 ccattagtaa ataaatcatt tttccaaaat aaacgttatg cttcattttt atttatagta
     2521 tttgtaatgt atgctatcca attgggttat atttttacgt tcccattcat aatggagcaa
     2581 atttatcatc tgcaactaga cacaacatca ctgttattag taccgggtta tatagtagca
     2641 gtcattgttg gtgcattaag tggtaaaatc ggcgaatatc tgaattcaaa acaagcgatt
     2701 atcacagcaa ttattttaat agcactgagc ttgattttac ctgcatttgc agtaggtaat
     2761 cacatttcaa tcttcgtcat ttctatgata ttctttgcag gtagctttgc tttaatgtat
     2821 gcacctttac ttaacgaagc cattaaaaca atagatctta atatgacagg tgtggctatt
     2881 ggtttttata atttaattat taatgtggcg gtatctgtag gtattgcgat tgctgcggct
     2941 ctaatcgatt ttaaagcatt aaatttccca ggcaatgatg cattaagttc acatttcggt
     3001 attattttaa ttattttagg tttaatgagt attgtcggat tagttttatt cgtcatctta
     3061 aatcgttgga cacaatctga aaaataaata gatataaatt cgcgagatat attcgtattt
     3121 atagtaaaat taaataaaga gattatataa cacgaggagt agtaagtatg aaatttgaga
     3181 aatatataga tcacacttta ttgaagcctg agtcaacacg tacgcaaa
//
LOCUS       DAHHSC010000477         1928 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.guided.295, whole genome shotgun sequence.
ACCESSION   DAHHSC010000477 DAHHSC010000000
VERSION     DAHHSC010000477.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1928)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1928)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1928
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.guided.295"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            214..1170
                     /locus_tag="NBI57_002879"
     CDS             214..1170
                     /locus_tag="NBI57_002879"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HCD1823750.1"
                     /translation="MLNSFDAAYHSLCEEVLEIGNTRNDRTNTGTISKFGHQLRFDLS
                     KGFPLLTTKKVSFKLVATELLWFIKGDTNIQYLLKYNNNIWNEWAFENYIKSDEYNGP
                     DMTDFGHRALSDPEFNEQYKEQMKQFKQRILEDDTFAKQFGDLGNVYGKQWRDWVDKD
                     GNHFDQLKTVIEQIKHNPDSRRHIVSAWNPTEIDTMALPPCHTMFQFYVQDGKLSCQL
                     YQRSADIFLGVPFNIASYALLTHLIAKECGLEVGEFVHTFGDAHIYSNHIDAIQTQLA
                     RESFNPPTLKINSDKSIFDINYEDLEIVDYESHPAIKAPIAV"
     gene            1370..1849
                     /locus_tag="NBI57_002880"
     CDS             1370..1849
                     /locus_tag="NBI57_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001830952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HCD1823751.1"
                     /translation="MTLSILVAHDLQRVIGFENQLPWHLPNDLKHVKKLSTGHTLVMG
                     RKTFESIGKPLPNRRNVVLTSDTSFNVEGVDVIHSIEDIYQLPGHVFIFGGQTLFEEM
                     IDKVDDMYITVIEGKFRGDTFFPPYTFEDWEVASSVEGKLDEKNTIPHTFLHLIRKK"
BASE COUNT          717 a          289 c          327 g          595 t
ORIGIN      
        1 agttatagat ttgggtaaac aattaccaat tggaaacata tatcacgtta cgatgtggta
       61 ggtacttaat cagcatttta taaataaagt aacggaattc atgatattaa tatcatattc
      121 ctaaaatgag tgataacaaa atgctacata aaattaagtt atatcaaact aaatatacat
      181 actataaata atgaaaatga ggtgttatcg catatgttga attcatttga tgcagcatat
      241 cacagtcttt gtgaagaggt tttagaaata ggaaatacac gaaatgatcg cacaaataca
      301 ggtacgattt cgaaatttgg tcatcaactt cgctttgact tatctaaagg atttccacta
      361 ttaacgacaa agaaagtttc ttttaaatta gtagcaaccg aattattatg gttcattaaa
      421 ggagatacaa acatccaata cttattaaaa tataataata atatttggaa cgaatgggct
      481 tttgaaaatt atataaaatc agacgagtat aacggtccag atatgacaga tttcgggcat
      541 cgtgcattga gtgatcctga atttaacgaa caatataaag aacaaatgaa acaatttaag
      601 caacgtattc ttgaagatga tacttttgcg aagcaattcg gtgatttagg aaatgtttat
      661 ggtaaacaat ggcgagattg ggttgataaa gatggtaatc attttgatca acttaaaaca
      721 gtaattgaac aaattaagca taatccagat tcaagacgac acatcgtatc tgcatggaat
      781 ccaacagaaa ttgatacaat ggcacttccg ccttgtcata ccatgttcca gttttatgtc
      841 caagatggta agttaagttg ccagttatac caacgtagcg cagatatctt tttaggtgtg
      901 ccatttaata tcgcaagcta cgctttattg acacacctta ttgccaaaga atgtggactt
      961 gaagtaggag aatttgtgca tacatttgga gatgcacata tttattcaaa tcatattgat
     1021 gcgattcaaa cacaattagc acgtgaaagc ttcaatcctc caacattaaa aattaacagt
     1081 gacaagtcta ttttcgacat aaattatgaa gatttggaaa ttgttgacta tgaatcacat
     1141 ccagcaataa aagctccaat agcagtgtag tcattgcata gttagctaac catatagaca
     1201 tcaaaatgac atcatagtat tttcaagtgc aaaaaagtac ttttttgtgt taaacgtttt
     1261 cataaattat gcaaaatcat tatttctatc acactttatg ataaaaattg tgttaaatta
     1321 aagataactt agtaataaaa aatgaaatga tagaagaagg aggataatta tgactttatc
     1381 cattctagtt gcacatgact tgcaacgagt aattggtttt gaaaatcaat taccttggca
     1441 cctaccaaat gatttgaaac atgttaaaaa attatcaaca ggtcatactt tagtaatggg
     1501 tcgtaagaca tttgaatcga ttggtaaacc actaccgaat cgtcgaaatg ttgtacttac
     1561 ttcagataca agtttcaacg tagagggcgt tgatgtaatt cactctattg aagatattta
     1621 ccaactaccg ggccatgttt tcatatttgg agggcaaaca ttatttgaag aaatgattga
     1681 taaagtggac gacatgtata ttactgttat tgaaggtaaa ttccgtggtg atacgttctt
     1741 tccaccttat acatttgaag actgggaagt tgcctcttca gttgaaggta aactagatga
     1801 gaaaaataca attccacata cctttctaca tttaattcgt aaaaaataag ggggaaaacg
     1861 accatgacaa aacagattat agtaacagac tcaacatccg atttatctaa agaatactta
     1921 gaagcaaa
//
LOCUS       DAHHSC010000478         1803 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.guided.307, whole genome shotgun sequence.
ACCESSION   DAHHSC010000478 DAHHSC010000000
VERSION     DAHHSC010000478.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1803)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1803)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1803
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.guided.307"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            <1..1404
                     /locus_tag="NBI57_002881"
     CDS             <1..1404
                     /locus_tag="NBI57_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class D beta-lactamase"
                     /protein_id="HCD1823752.1"
                     /translation="FKFLKSLLYLKYLKKQSLYLNENEKNKIDTILFNHQYKKNIVIR
                     KAETIQSPITFWYGKYIILIPSSYFKSVIDKRLKYIILHEYAHAKNRDTLHLIIFNIF
                     SIVMSYNPLIHIVKRKIIHDNEVEADRFVLNNINKNEFKTYAESIMDSVLNIPFFNKN
                     ILSHSFNGKKSLLKRRLINIKEANLKKQSKLIPIFICIFTFLLMVIQSQFLMGQSITD
                     YNYKKPLQNDHQILDESKNFGSNSGSFVMYSMKKDKYYIYNEKESRKRYSPDSTYKIY
                     LAMFGLDHHIISDKNSRMSWNHKHYPFESWNKEQDLNTAMQNSVNWYFERISNQIPKN
                     YTAAQLKQLNYGNENLGSYKSYWMEDSLKISNLEQVIVFKNMMEQNNHFSKKAKNQLS
                     SSLLIKKNEKYELYGKTGTGIVNGKYNNGWFVGYVITNHDKYYFATHLSDGKPSGKNA
                     ELISEKILKEMGVLNGQ"
     gene            1394..1774
                     /gene="blaI"
                     /locus_tag="NBI57_002882"
     CDS             1394..1774
                     /gene="blaI"
                     /locus_tag="NBI57_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillinase repressor BlaI"
                     /protein_id="HCD1823753.1"
                     /translation="MANKQVEISMAEWDVMNIIWDKKSVSANEIVVEIQKYKEVSDKT
                     IRTLITRLYKKEIIKRYKSENIYFYSSNIKEDDIKMKTAKTFLNKLYGGDMKSLVLNF
                     AKNEELNNKEIEELRDILNDISKK"
BASE COUNT          764 a          198 c          266 g          575 t
ORIGIN      
        1 tttaaatttt tgaaatcatt attatatctt aaatatttaa agaaacagtc actttatcta
       61 aacgaaaatg aaaaaaataa aatagatacg atacttttca accatcaata taaaaaaaat
      121 attgtgattc gaaaagcaga gactattcaa tctccaataa ctttttggta tgggaaatat
      181 attattttga ttcctagttc atattttaaa agtgtaattg ataaaagact aaaatatata
      241 atactgcatg aatatgctca tgctaaaaat agagatactt tacatttaat tatctttaat
      301 atcttctcta ttgttatgag ttacaatcca ttaatacata ttgtaaaaag aaagataatt
      361 catgacaatg aagtagaagc cgatagattt gttcttaata atattaacaa aaatgaattt
      421 aaaacttatg cggaatctat tatggactcc gtattaaata ttccattttt taataaaaat
      481 atattaagcc attcatttaa tggtaaaaag tcattactca aaagaagatt aattaatata
      541 aaagaagcca atttgaaaaa acagtccaaa ttaattccaa tttttatttg tatttttact
      601 tttttgctca tggtaattca aagccagttt ttgatggggc aatccataac tgattataat
      661 tataaaaagc ccttacagaa cgatcaccaa attttagatg aaagtaaaaa ttttggttca
      721 aatagtggat ctttcgtcat gtacagcatg aagaaagaca aatactatat ttataatgaa
      781 aaagaaagta gaaaaagata ttctcctgat tcaacttata aaatttattt agcgatgttt
      841 ggacttgacc atcatatcat aagtgataaa aattcacgta tgtcatggaa tcataaacat
      901 tatcctttcg agtcttggaa taaggaacaa gatttaaata cagcaatgca aaattcagtt
      961 aattggtact tcgaacgtat tagtaatcaa ataccaaaga actatactgc ggctcaactc
     1021 aagcaattaa attatggtaa tgaaaacttg ggaagttata aaagctattg gatggaagat
     1081 agtttgaaaa tatctaatct tgaacaagta atagttttta aaaatatgat ggaacaaaat
     1141 aaccatttta gtaaaaaagc aaagaatcaa ttatcttctt cattattgat taagaaaaat
     1201 gaaaagtatg aactgtatgg gaaaacaggt acaggtatag taaacgggaa gtataataat
     1261 gggtggtttg taggttacgt aattacaaat catgataagt attattttgc tacacattta
     1321 tcagatggaa agccgtctgg gaaaaatgct gaattaatca gtgaaaaaat attaaaagaa
     1381 atgggtgttt taaatggcca ataagcaagt tgaaatatct atggctgaat gggatgttat
     1441 gaatataata tgggataaaa aatcagtatc agctaatgaa attgtagttg aaattcaaaa
     1501 atataaagaa gttagcgata aaacgattag aacattaatc acaagactat ataaaaaaga
     1561 gattataaaa cgatacaaat cagagaatat ttatttttac tcatcaaata ttaaagaaga
     1621 cgatattaaa atgaaaactg ctaaaacctt tcttaataaa ctgtatggag gggacatgaa
     1681 aagtttagtg ctgaattttg cgaaaaatga agaattaaat aacaaagaaa ttgaagaatt
     1741 gcgagacatt ttaaatgata ttagtaaaaa gtaattagag atatgtttca aacataatac
     1801 ata
//
LOCUS       DAHHSC010000479         1675 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.guided.315, whole genome shotgun sequence.
ACCESSION   DAHHSC010000479 DAHHSC010000000
VERSION     DAHHSC010000479.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1675)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1675)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1675
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.guided.315"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            complement(<1..358)
                     /locus_tag="NBI57_002883"
     CDS             complement(<1..358)
                     /locus_tag="NBI57_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactam sensor/signal transducer BlaR1"
                     /protein_id="HCD1823754.1"
                     /translation="MAKLLITSVVSFCFIFLLLVFFKYILKRYFNYSLNYKVWYLTVL
                     AGLIPFIPIKFSFIKLNNVNNQAPTVESKSHDLNHNINTTKPVQEFTTDIHKFNWDSI
                     DNICTVVWIVLVIILSF"
     gene            465..1310
                     /gene="blaZ"
                     /locus_tag="NBI57_002884"
     CDS             465..1310
                     /gene="blaZ"
                     /locus_tag="NBI57_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000733274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-hydrolyzing class A beta-lactamase
                     BlaZ"
                     /protein_id="HCD1823755.1"
                     /translation="MKKLIFLIAIALVLSACNSNSPHAKKLNDLEKKYNAHIGVYALD
                     TKSGKEVKFNSDKRFAYASTSKAINSAILLEQIPYNKLNKKIHINKDDIVAYSPILEK
                     YVGKDITLKELIEASMTYSDNTANNKIIKEIGGIKKVKQRLKELGDKVTNPVRYEIEL
                     NYYSPKSKKDTSTPAAFGKTLNKLIANGKLSKENKKFLLDLMLNNKSGDTLIKDGVSK
                     DCKVADKSGQAITYASRNDVAFVYPKGQSEPIVLVIFTNKDNKSDKPNDKLISETAKS
                     VMKEF"
BASE COUNT          673 a          200 c          234 g          568 t
ORIGIN      
        1 taaaacttaa aataataact aaaactatcc aaacaactgt gcagatatta tcaattgaat
       61 cccaattaaa cttatggata tctgttgtga actcttgaac aggtttggtg gtatttatgt
      121 tatggttcaa gtcgtgtgac ttactttcaa ctgtgggcgc ttgattattc acattattca
      181 attttataaa agagaattta attgggataa aaggaattaa tccagcaagc acagttagat
      241 accaaacttt ataatttaaa ctataattaa aatagcgttt taatatgtac ttaaaaaata
      301 ctaacaatag aaatataaaa caaaagctta ctacgctcgt tattagtaat ttagccattt
      361 tgacaccttc tttcaaatat ttataataaa caattgacac cgatattaca attgtaatat
      421 tatttattta taaaaattac aattgtaata ttggagggtt tattttgaaa aagttaatat
      481 ttttaattgc aattgcttta gttttaagtg catgtaattc aaacagtcca catgccaaaa
      541 agttaaatga tttagaaaaa aaatataatg ctcatattgg tgtttatgct ttagatacta
      601 aaagtggtaa ggaagtaaaa tttaattcag ataagagatt tgcctatgct tcgacttcaa
      661 aagcgataaa tagtgctatt ttgttagaac aaatacctta taataagtta aataaaaaaa
      721 tacatattaa caaagatgat atagttgctt attctcctat tttagaaaaa tatgtaggaa
      781 aagatatcac tttaaaagaa cttattgagg cttcaatgac atatagtgat aatacagcaa
      841 acaataaaat tataaaagaa atcggtggga tcaaaaaagt taaacaacgt ctaaaagaac
      901 taggagataa agtaacaaat ccagttagat atgagataga attaaattac tattcaccaa
      961 agagcaaaaa agatacttca acacctgctg ctttcggtaa gactttaaat aaacttatcg
     1021 caaatggaaa attaagcaaa gaaaacaaaa aattcttact tgatttaatg ttaaataata
     1081 aaagcggaga tactttaatt aaagatggtg tttcaaaaga ctgtaaggtg gctgataaaa
     1141 gtggtcaagc aataacatat gcttctagaa atgatgttgc ttttgtttat cctaagggcc
     1201 aatctgaacc tattgtttta gtcattttta cgaataaaga caataaaagt gataagccaa
     1261 atgataagtt gataagtgaa accgccaaga gtgtaatgaa ggaattttaa tattctaaat
     1321 gcataataaa tactgatatc atcttatagt ttgtattatc gttgacatct ataattttga
     1381 tatcaaaaac tgattttccc tctattattt tcgagattta ttttcttaat tctctttaac
     1441 aaactagaaa tattgtatat acaaaaaatc ataaataata gatgaatagt ttaattctag
     1501 gtattcatca atcgaaaaag taacgtatct tatttaaagt gcgttgcttt tttctcattt
     1561 ataaggttaa ataattctca tatatcaagc aaagtgacaa acaacttatt ttgtgtgatt
     1621 agggtgtact taaataacac aaaagtaggt ttaatataat taattatatt aaatt
//
LOCUS       DAHHSC010000480          608 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M99412
            SAMN28743617-rid14503783.guided.423, whole genome shotgun sequence.
ACCESSION   DAHHSC010000480 DAHHSC010000000
VERSION     DAHHSC010000480.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743617
            Sequence Read Archive: SRR19441838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 608)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 608)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316827.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316827.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:49:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,756
            CDSs (with protein)               :: 2,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 38 of 64
            Pseudo Genes (incomplete)         :: 19 of 64
            Pseudo Genes (internal stop)      :: 20 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..608
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743617-rid14503783.guided.423"
                     /isolate="M99412"
                     /isolation_source="Perianal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-09-17"
     gene            127..546
                     /locus_tag="NBI57_002885"
     CDS             127..546
                     /locus_tag="NBI57_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FosB1/FosB3 family fosfomycin resistance
                     bacillithiol transferase"
                     /protein_id="HCD1823756.1"
                     /translation="MLKSINHICFSVRNLNDSIHFYRDILLGKLLLTGKKTAYFELAG
                     LWIALNEEKDIPRNEIHFSYTHIAFTIDDSEFKYWHQRLKDNNVNILEGRVRDIRDRQ
                     SIYFTDPDGHKLELHTGTLENRLNYYKEAKPHMTFYK"
BASE COUNT          241 a           78 c           90 g          199 t
ORIGIN      
        1 tttaacttaa gttattagag cctcttatgc agttgctcag atcatcgtat aaaaattaat
       61 gacgtcattt caaaaatcga tacaaaaata atttattata aaaattctaa gaaagaagtg
      121 aagcagatgt taaaatctat taatcatata tgcttttcag tcagaaattt aaacgattca
      181 atacattttt atagagatat tttacttggg aaattgctat tgactggtaa aaaaactgct
      241 tattttgagc ttgcaggcct atggattgct ttaaatgaag aaaaagatat accacgtaat
      301 gaaattcact tttcatatac acatatagct ttcactatag atgacagcga atttaaatat
      361 tggcatcaga ggttaaaaga taataacgtg aatattttag aaggaagagt tagagatatt
      421 agagatagac aatcaattta ctttactgac cctgatggtc ataagctaga attacatact
      481 ggcacacttg agaacagatt aaattattat aaagaggcta aaccacatat gacattttac
      541 aaataaggcg tcattataaa aaggcctctt gaaatccgtt aaaattttaa ttaattatta
      601 tataataa
//
